The Spider-Slayers are a series of fictional robots appearing in American comic books published by Marvel Comics. Created by Spencer Smythe, they were designed specifically to hunt down, capture, or kill Spider-Man. A new generation of Spider-Slayers was later created by Spencer's son, Alistair. The Spider-Slayers have been featured in a number of Spider-Man adaptations outside of comics, including animated series and video games. Publication history The Spider-Slayers first appeared in The Amazing Spider-Man #25 (June 1965) and were created by Stan Lee and Steve Ditko. Fictional character biography See List of Spider-Slayers for a full breakdown of all versions of the Spider-Slayer. The first series of robots were originally designed and built by Prof. Spencer Smythe with the financial backing of J. Jonah Jameson, who piloted them for the pleasure of personally hunting the superhero he hated for capture and public unmasking. However, Spider-Man always managed to defeat each robot in turn. Smythe's continual efforts to perfect his machines backfired on him, leading to himself being fatally contaminated by radiation poisoning from the building materials of his creations. Blaming both Jameson and Spider-Man for this, he attempted, as a final act, to murder them both, but died just before Spider-Man thwarted the attempt. At one point, Jameson commissioned another scientist, Dr. Marla Madison, to create a new and improved Spider-Slayer. While that attempt failed, he eventually fell in love with her and married her. In The Amazing Spider-Man Annual #19 (1985), Smythe's son, Alistair, emerged as the new builder of the Spider-Slayers. He swore revenge on Spider-Man, repeatedly attacking the superhero with his own series of Slayers. Smythe later mutated into a humanoid Spider-Slayer, but remains a minor foe. The original Spider-Slayer was seen among the robots and machines in the Reanimator's collection. Wolverine later destroyed the Spider-Slayer when the Reanimator attempted to use it against him. It was later used by J. Jonah Jameson to attack the She-Hulk after she had married his son John, but it was destroyed again. In The Amazing Spider-Man #603, Jameson (now Mayor of New York) has some old Spider-Slayers sent to him from storage to better equip his "Anti-Spider Squad" to take down Spider-Man. The Spider-Slayer technology is combined with that of the Mandroid suits. However, the "Spider-Slayer Squad" wearing the suits quit their jobs after Spider-Man saves them and New York from a dirty bomb. Despite not being technically related to