The point spread function (PSF) describes the response of a focused optical imaging system to a point source or point object. A more general term for the PSF is the system's impulse response; the PSF is the impulse response or impulse response function (IRF) of a focused optical imaging system. The PSF in many contexts can be thought of as the extended blob in an image that represents a single point object, that is considered as a spatial impulse. In functional terms, it is the spatial domain version (i.e., the inverse Fourier transform) of the optical transfer function (OTF) of an imaging system. It is a useful concept in Fourier optics, astronomical imaging, medical imaging, electron microscopy and other imaging techniques such as 3D microscopy (like in confocal laser scanning microscopy) and fluorescence microscopy. The degree of spreading (blurring) in the image of a point object for an imaging system is a measure of the quality of the imaging system. In non-coherent imaging systems, such as fluorescent microscopes, telescopes or optical microscopes, the image formation process is linear in the image intensity and described by a linear system theory. This means that when two objects A and B are imaged simultaneously by a non-coherent imaging system, the resulting image is equal to the sum of the independently imaged objects. In other words: the imaging of A is unaffected by the imaging of B and vice versa, owing to the non-interacting property of photons. In space-invariant systems, i.e. those in which the PSF is the same everywhere in the imaging space, the image of a complex object is then the convolution of that object and the PSF. The PSF can be derived from diffraction integrals. Introduction By virtue of the linearity property of optical non-coherent imaging systems, i.e., Image(Object1 + Object2) = Image(Object1) + Image(Object2) the image of an object in a microscope or telescope as a non-coherent imaging system can be computed by expressing the object-plane field as a weighted sum of 2D impulse functions, and then expressing the image plane field as a weighted sum of the images of these impulse functions. This is known as the superposition principle, valid for linear systems. The images of the individual object-plane impulse functions are called point spread functions (PSF), reflecting the fact that a mathematical point of light in the object plane is spread out to form a finite area in