have an extra carbon 7, carbon 8 epoxide group. The common example of these is crotocin. which also has an ester functional group at carbon 4. Type D trichothecenes have an additional ring between carbon 4 and carbon 15. These rings can have diverse additional functional groups. Common examples of these are roridin A and satratoxin H. Although the distinct functional groups of these classification types give each trichothecene unique chemical properties, their classification type does not explicitly indicate their relative toxicity. While Type D trichothecenes are thought to be the most toxic, Types A and B have relatively mixed toxicity. Alternative classifications The classification system described above is the most commonly used to group molecules of the trichothecene family. However, a variety of alternative classification systems also exist for these complex molecules. Trichothecenes can also be generally described as simple or macrocyclic. Simple trichothecenes include Types A, B, and C, whereas macrocyclic tricothecenes include Type D and are characterized by the presence of a carbon 4 - carbon 15 bridge. Additionally, J. F. Grove proposed a classification of tricothecenes into three groups that was also based upon the functional substitution patterns of the ring skeleton. Group 1 tricothecenes only have functional groups substituted on the third, fully saturated carbon ring. Group 2 tricothecenes contain additional functional groups on the core ring containing the 9, 10 carbon double bond. Finally, Group 3 trichothecenes contain a ketone functional group at carbon 8; this is the same criteria for Type B trichothecenes. Advances in the field of evolutionary genetics have also led to the proposal of trichothecene classification systems based on the pathway of their biosynthesis. Genes responsible for the biosynthesis of a mycotoxin are typically located in clusters; in Fusariumi these are known as TRI genes. TRI genes are each responsible for producing an enzyme that carries out a specific step in the biosynthesis of trichothecenes. Mutations in these genes can lead to the production of variant trichothecenes and therefore these molecules could be grouped on the basis of shared biosynthesis steps. For example, a shared step in the biosynthesis of trichothecenes is controlled by the gene TRI4. This enzyme product controls the addition of either three or four oxygens to trichodiene to form either isotrichodiol or isotrichotriol respectively. A variety of trichothecenes can then be synthesized from either of these intermediates and they could therefore be classified as either t-type