filled with plentiful vegetation. The species is found in natural and settled environments. The species commonly resides in cypress ponds, water lily prairies, and marshes. They are often found perched on twigs, low branches, and grasses. Conservation A growing number of American green tree frogs have experienced severe habitat loss primarily due to urbanization and destructive wildfires that can destroy forest canopy cover. Since most amphibians have narrow habitat tolerances and migration constraints, American green tree frogs urgently need alternative shelters for survival as forest canopies recover. In a study from Central Texas, scientists have tried to combat wildfire habitat loss by creating artificial shelters using PVC pipes. Population structure, speciation, and phylogeny One study finds that there are at least 31 tree frog species of the genus Hyla (or Dryophytes) in North America, Central America, and Eurasia. Examples include both the H. gratiosa and H. walkeri. While many tree frogs reside in the New World, a notable number of frogs inhabit the Eurasia continent and display unique biogeographic patterns based on an analysis of nuclear and mitochondrial DNA sequences. Home range and territoriality American green tree frogs will defend their mate calling sites against foreign rivals and invaders using aggressive interactions. Such behaviors include a combination of aggressive call signals and wrestling from males. Diet American green tree frogs are insectivores, primarily consuming flies, mosquitoes, grasshoppers, cockroaches, spiders, beetles, and other small insects such as crickets and ants. One study suggested frogs select prey not by their size, but according to their activity levels, with the most active prey being the most frequently eaten. The same study showed "nearly 90% of Hyla cinerea prey were actively pursued", with the other 10% being "insects walking or close enough to be snatched up by the frog's tongue". Behavior Because the species is small and easily frightened, they often does not do well with frequent handling. Some specimens do seem to tolerate it occasionally, so handling frequency should be determined on an individual basis. The American green tree frog tends to be nocturnal, so they will be most active once the lights are off. Males call most of the year, especially after being misted in their tank. Breeding Most American green tree frog females breed once per year, but some have multiple clutches in a single mating season. In a Florida population, "advertisement calls of males were documented between March and September