and pairs in amplexus were observed between April and August". The average number of eggs in a single clutch was observed to be about 400 for this specific population. Eggs take between four and 14 days to hatch, with an average of five days. According to the Animal Diversity Web at the University of Michigan Museum of Zoology, "Female size was positively correlated with clutch size, but after the initial clutch, the number of eggs nearly always decreased". Tadpoles are green with a yellow or white stripe extending from each nostril to the eye and may have mottled tail fins. American green tree frogs show only the parental investment of mating and egg-laying. Breeding is known to be strongly influenced by day length, temperature, and precipitation. While the influence of these factors with respect to breeding is not well understood, it is well documented, as the frogs generally breed following rainfall and males call more frequently as temperature and day length increase. Some evidence demonstrates that the length of the breeding season is correlated with latitude; seasonal length decreases as latitude increases due to temperature limitations. Mating calls To attract mates, the male American green tree frog uses a distinctive advertisement call which is noticeably different from its release or warning calls. Once a mate has been attracted, the pair begins amplexus in which the male frog grasps onto the female to initiate fertilization. The species is polygynous, with the male generally seeking to mate with as many females as it can attract. Eggs are attached to substrates such as emergent vegetation, and unlike other frog species, these egg masses are typically laid in permanent bodies of water rather than vernal pools. When male frogs aggregate, choruses will form and establish a cacophony of numerous unique advertisement calls. Consequently, male individuals experience intraspecific mating competition and often encounter immense pressure to produce unique call signals that are both attractive and audible to a limited number of available females. Such challenges are further complicated by the rapid fluctuation of males within a chorus, the potential risk of increased exposure to predators, and sexual selection of specific call signals through female choice. These factors give rise to a social plasticity in the calling behavior of the American green tree frog. In order to maintain competition, male individuals will either modify their signal features, such as the temporal and spectral properties of calls