1X PBS and a quick wash of dH2O. The AB9 cells were mounted with vectashield and were examined under Nikon Eclipse TE2000-U at 60X. Functional Assays in AB9 Cells Knocking down a protein mean that an organism's genes are reduced. To knockdown AB9 the first thing that is test is if electroporation is a reliable method for uptake of MO or antisense morpholinos. We begin by electrophoralting fluorescein labeled cx43 MO into AB9 cells, using fluorescein labeled standard control MO as the control. We observe that the efficiency of 50-70%. Next we test the MO uptake of AB9 led by protein knockdown. Lysates were prepared from Standard curve and cx43 Mo treated cells at 25 hpe. Next, we try to determine if we can block protein function by treating AB9 with pharmacological agents. The inhibitor in this case will be Hsp47 and we treat the cell with 100 um of the inhibitor, using DMSO control. References External links Cellosaurus entry for AB9 Fish cell lines Danios