below. Multipotentectomesenchymal Precursor Cells Multipotentectomesenchymal precursor cells also referred to as DFCs are found in the coronal part of the DF of impacted human third molar teeth contains. DFCs are considered multipotent, and they are especially precursors for cells of the tooth attachment apparatus Cells of this tissue structure express typical markers for PDL fibroblast, alveolar osteoblasts, and cementoblasts. When cultivated, DFCs have a morphology that is comparable to that of a fibroblast and express markers such as Nestin and STRO-1 which are typical markers for dental stem cells. These cells have are highly proliferate and proliferate typically at a higher rate than that of a proliferating bone marrow- derived Mesenchymal stem cell. Regulation of DFCs The initiation of differentiation is controlled by a number of different extracellular factors, such as growth factors, cell-cell contacts, extracellular matrix, and mechanical loading. These factors coordinate to induce or modulate the process of differentiation into particular functional body cell type. There have been some studies done recently about the differentiation of cultivated DFCs into biomineralising cells. These studies revealed new ways in which the cell differentiation mechanisms work. Moreover, information about genome-wide expression profiles was provided by proteomics and transcriptomics with DFCs. These help in showing more clearly the molecular mechanisms in cells. The extracellular signal regulated kinase (ERK) pathway was also revealed during the osteogenic differentiation of DFCs by these investigations. The proteomics and transcriptomics identified regulated transcription factors such as SP1 and TP53. These transcription factors were more precisely identified by bioinformatics after the analysis of the proteome. The role of these transcription factors regulate the cell proliferation and differentiation of DFCs. Human dental follicle cells are progenitor cells. Different studies suggested that osteogenic differentiation of DFCs is controlled by BMP2 and IGF2, which are growth factors. However, the influence of BMP2 and IGF2 on the differentiation of DFCs has not been analysed in too much depth. There was a study that examined DFCs after the induction of osteogenic differentiation with BMP2, IGF2 and a standard osteogenic differentiation medium (ODM) with dexamethasone. The alkaline phosphatase activity and the calcium accumulation showed osteogenic differentiation after all treatments, but with the most effective differentiation by ODM. Furthermore, markers of the process of osteoblast differentiation were much higher up-regulated in BMP2- or IGF2-treated cells than in ODM-treated cells. To find the reason between these differences, the genome-wide expression profiles were compared at an early