as In many cases, scientists may use both SSMD and average fold change for hit selection in HTS experiments. The dual-flashlight plot can display both average fold change and SSMD for all test compounds in an assay and help to integrate both of them to select hits in HTS experiments . The use of SSMD for hit selection in HTS experiments is illustrated step-by-step in See also Effect size high-throughput screening Z-factor Hit selection SMCV c+-probability Contrast variable Dual-flashlight plot Further reading Zhang XHD (2011) "Optimal High-Throughput Screening: Practical Experimental Design and Data Analysis for Genome-scale RNAi Research, Cambridge University Press" References Effect size Descriptive statistics