Broadway Street Jane Doe, and Lyle Stevik cases. Amounts of available DNA too small for adequate testing, especially those involving difficult bone extractions. Multiple extractions may be required to obtain a suitable sample. Degraded DNA. This was a condition encountered in the Joseph Newton Chandler III case, as well as with Sue Ann Huskey and Tamara Tigard. Bacterial/human contamination reducing the amounts of Doe's DNA that can be used for analysis, a problem not usually discovered until sequencing is complete. James Freund and Pamela Buckley's DNA was contaminated with bacteria, and two Does from Washington had their investigations put on hold due to contamination. Exceedingly large family trees, which can cause investigations to take weeks or months. This was the case with Joseph Henry Loveless, Karen Knippers, and Kings County and Kern County (2011) Jane Doe and Richard Bunts/Bunce, born in 1793 New York. Richard's case was very difficult because of bacterial contamination which was on his body, for many years. In 2020, Margaret Press stated that the COVID-19 pandemic had hampered investigations, particularly those that had been solved and now needed law enforcement to make contact with the families. She stated that many agencies prefer to do these announcements in person but had not been able due to the pandemic and its associated travel restrictions. She added that volunteers and laboratories had also been affected by the pandemic. Cases 2018 identifications Marcia King, "Buckskin Girl" In 1981, three passerbys found a female murder victim in a ditch in Troy, Ohio. Because the victim was found wearing a distinctive buckskin coat, she was given the name "Buckskin Girl" as the investigation continued. For decades, authorities sought the woman's identity, but to no avail. At the 2017 American Academy of Forensic Sciences conference, Elizabeth Murray, an Ohio forensic anthropologist, met Colleen M. Fitzpatrick and Margaret Press, founders of the DDP, who discussed what genetic genealogy techniques could do for this case. The victim's body had long since been buried, but a vial of blood had been held in a lab for 37 years. The vial had not been refrigerated, however, resulting in the DNA becoming highly degraded, with only 50 to 75 percent of markers remaining. With the help of Greg Magoon, a senior researcher at Aerodyne Research, they were able to upload this DNA data to GEDmatch. From this point, the DDP was able to identify the "Buckskin Girl", based