concentrations during a manufacturing process Analyse many proteins at once Measure low abundant HCPs overshadowed by the high abundant target protein product Recently, the MS method has been further improved through the method SWATH LC-MS. SWATH is a data independent acquisition (DIA) form of mass spectrometry, where the mass range is partitioned in small mass windows, which is then analysed with tandem MS (MS/MS). The key advantages are the reproducibility for both individual HCP identification and absolute quantification by applying internal protein standards. Despite the solid improvements of this method of protein analysis, there are also limitations, the main of which is to take into account the properties of the sample matter, thus: sampling conditions, limited technical variability, and the number of missing values in the sample - lead to infelicity that can be challenged in statistical analysis by factoring in batch effects, sampling effects, and the increase in missing identifications. See also Biomolecular engineering Liquid chromatography–mass spectrometry Mass spectrometry Protein production Protein purification Recombinant DNA References Biotechnology