is a mesophile. The Oscillochloris (Class Chloroflexia) are also mesophilic. Despite this relationship, D. lykanthroporepellens is more closely related to the Dehalococcoides (class Dehalococcoidia) with 90% 16S rRNA gene sequence similarity. D. lykanthroporepellens also differ from other species in the phylum Chloroflexota in that they are not filamentous. Metabolism Dehalogenimonas lykanthroporepellens is a chemotrophic organism that uses H2 as an electron donor and polychlorinated aliphatic alkanes as an electron acceptor. These molecules include 1,2,3-trichloropropane, 1,2-dichloropropane, 1,1,2,2-tetrachloroethane, 1,1,2-trichloroethane, and 1,2-dichloroethane. However, there are several chlorinated alkanes that it cannot reduce, such as 1-chloropropane and 2-chloropropane. It uses these compounds as electron acceptors in dihaloelimination reactions. In dihaloelimination the electron donor (H2 in this case) is used to remove two halogens from adjacent carbons that are double bonded. 1,2,3-trichloropropane is reduced to allyl chloride by D. lykanthroporepellens, and further transformed abiotically to allyl alcohol in the presence of water (other abiotic reactions can occur). The carbon source has not been determined for this species but other organisms within Chloroflexota use CO2 as a carbon source. Genome Although two strains of D. lykanthroporepellens have been isolated and characterized, only the type strain BL-DC-9T has had the genome sequenced. Therefore, when referring to D. lykanthroporepellens in this section, all information is only verified for BL-DC-9T. D. lykanthroporepellens has a circular chromosome consisting of 1,686,510 bp and a G-C content, based on genomic analysis, of 55.04%. The genome was sequenced using both Illumina and 454 sequencing platforms, more specifically an Illumina shotgun library, a 454 draft library, and a paired end 454 library. Illumina sequence data was assembled and combined with assembled 454 data. Initial assembly contained 64 contigs (a set of overlapping DNA) in 1 scaffold (a set of overlapping contigs with known gap lengths). Genes were annotated using a combination of automated and manual curation. 1,771 genes were predicted, in which 1,720 were protein-coding genes and 51 were RNAs. Putative function was designated to nearly 70% of the protein-coding genes. Interest in D. lykanthroporepellens stems from its ability to degrade polychlorinated aliphatic alkanes into nonhazardous products. The catalysis of reductive dehalogenation of chlorinated compounds is dependent on the presence and expression of genes coding for reductive dehalogenase enzymes. These genes are organized in rdhAB operons, which encode the RdhA protein (reductive dehalogenase) and the RdhB protein (membrane anchor). D. lykanthroporepellens was shown to have several rdhA and rdhB genes in the chromosome. Furthermore,