Effect Spectroscopy TRNOE, Transferred Nuclear Overhauser Effect DPFGSE-NOE, Double Pulsed Field Gradient Spin Echo NOE experiment NOESY is the determination of the relative orientations of atoms in a molecule, for example a protein or other large biological molecule, producing a three-dimensional structure. HOESY is NOESY cross-correlation between atoms of different elements. ROESY involves spin-locking the magnetization to prevent it from going to zero, applied for molecules for which regular NOESY is not applicable. TRNOE measures the NOE between two different molecules interacting in the same solution, as in a ligand binding to a protein. In a DPFGSE-NOE experiment, a transient experiment that allows for suppression of strong signals and thus detection of very small NOEs. Examples of nuclear Overhauser effect The figure (top) displays how Nuclear Overhauser Effect Spectroscopy can elucidate the structure of a switchable compound. In this example, the proton designated as {H} shows two different sets of NOEs depending on the isomerization state (cis or trans) of the switchable azo groups. In the trans state proton {H} is far from the phenyl group showing blue coloured NOEs; while the cis'' state holds proton {H} in the vicinity of the phenyl group resulting in the emergence of new NOEs (show in red). Another example (bottom) where application where the NOE is useful to assign resonances and determine configuration is polysaccharides. For instance, complex glucans possess a multitude of overlapping signals, especially in a proton spectrum. Therefore, it is advantageous to utilize 2D NMR experiments including NOESY for the assignment of signals. See, for example, NOE of carbohydrates. See also Dynamic nuclear polarization Magnetization Nuclear magnetic resonance Nuclear magnetic resonance spectroscopy Nuclear magnetic resonance spectroscopy of proteins Proton nuclear magnetic resonance Spin polarization Two-dimensional nuclear magnetic resonance spectroscopy References External links Hans J. Reich: The Nuclear Overhauser Effect Eugene E. Kwan: Lecture12: The Nuclear Overhauser Effect Beat Vögeli: Nuclear Overhauser Effect in NMR Structure and Dynamics Analysis Williams, Martin and Rovnyak Vol 2: R. R. Gil and A. Navarro-Vázquez: Chapter 1 Application of the Nuclear Overhauser Effect to the Structural Elucidation of Natural Products James Keeler: 8 Relaxation YouTube: James Keeler, Lecture 10, Relaxation II. 2013 Cambridge lecture on NOE Arthur G. Palmer, III: Relaxation and Dynamic Processes Nuclear magnetic resonance spectroscopy Nuclear magnetic resonance Chemical physics