°C for 30 minutes or 72 °C for 15 seconds. Certain pathogens must be heated above boiling (e.g. botulismClostridium botulinum requires , most endospores require , and prions even higher). Higher temperatures may be achieved with a pressure cooker. Heat combined with ultraviolet light (UV), such as sodis method, reduces the necessary temperature and duration. Filtration Portable pump filters are commercially available with ceramic filters that filter 5,000 to 50,000 litres per cartridge, removing pathogens down to the 0.2–0.3 micrometer (µm) range. Some also utilize activated charcoal filtering. Most filters of this kind remove most bacteria and protozoa, such as Cryptosporidium and Giardia lamblia, but not viruses except for the very largest of 0.3 µm and larger diameters, so disinfection by chemicals or ultraviolet light is still required after filtration. It is worth noting that not all bacteria are removed by 0.2 µm pump filters; for example, strands of thread-like Leptospira spp. (which can cause leptospirosis) are thin enough to pass through a 0.2 µm filter. Effective chemical additives to address shortcomings in pump filters include chlorine, chlorine dioxide, iodine, and sodium hypochlorite (bleach). There have been polymer and ceramic filters on the market that incorporated iodine post-treatment in their filter elements to kill viruses and the smaller bacteria that cannot be filtered out, but most have disappeared due to the unpleasant taste imparted to the water, as well as possible adverse health effects when iodine is ingested over protracted periods. While the filtration elements may do an excellent job of removing most bacteria and fungi contaminants from drinking water when new, the elements themselves can become colonization sites. In recent years some filters have been enhanced by bonding silver metal nanoparticles to the ceramic element and/or to the activated charcoal to suppress growth of pathogens. Small, hand-pumped reverse osmosis filters were originally developed for the military in the late 1980s for use as survival equipment, for example, to be included with inflatable rafts on aircraft. Civilian versions are available. Instead of using the static pressure of a water supply line to force the water through the filter, pressure is provided by a hand-operated pump. These devices can generate drinkable water from seawater. The Portable Aqua Unit for Lifesaving (short PAUL) is a portable ultrafiltration-based membrane water filter for humanitarian aid. It allows the decentralized supply of clean water in emergency and disaster situations for about 400 persons per unit