coli O157:H7 strain. These cases were reported from October 15, 2021 through October 27, 2021 and an investigation was carried out by the Minnesota Department of Agriculture and FDA. It was concluded that packages of spinach collected from homes of infected people were contaminated with a strain of E. coli that matched the strain causing illness. This was determined by performing whole genome sequencing on the strain extracted from the spinach and comparing it to the strain taken from infected individuals. As of February 7th, 2022, the provinces of Alberta and Saskatchewan in Canada reported a collective fourteen confirmed cases of E. coli O157 strain illnesses. These were reported between December 2021 and January 2022, and the Public Health Agency of Canada (PHAC), the Canadian Food Inspection Agency (CFIA), and Health Canada were able to determine a specific brand of Original Kimchi to be the source of the organism. On January 28, 2022 and February 6, 2022, the CFIA issued a recall on Hankook Original Kimchi. Detection of Coliform Bacteria in Drinking Water PCR Amplification of the beta-galactosidase gene is used to detect coliforms in general, because all coliform organisms produce this compound. The amplification of the beta-D glucuronidase is used to detect E. coli, or the amplification of their verotoxin gene(s) to detect verotoxin-producing E. coli. Chemiluminescent in-situ hybridization Specific areas of the 16S rRNA in the Enterobacteriaceae genus are bound by oligonucleotide probes, which aids in monitoring the quality of drinking water. Specifically, E. coli is labelled with a soybean peroxidase-labeled peptide nucleic acid (PNA) probes that bind to a specific sequence in their 16S rRNA. When used in conjunction with a chemiluminescent substrate, light is produced where each colony of E. coli is located, indicating that they are present in the sample. Violet red bile agar The solid medium is used to grow lactose-fermenting coliforms and utilizes a neutral red pH indicator. Pink colonies appear when lactose is fermented and are surrounded by bile that has precipitated out. To confirm if these colonies are coliforms, they are transferred to brilliant green lactose bile (BGLB) and incubated. If gas is visible after incubation, it can be confirmed that the sample had coliforms present. Membrane filter method Test samples are filtered through standard filter paper and then transferred to M-endo or LES Endo Agar mediums. Colonies appear pinkish-red with green metallic sheen after 22-24 hours of incubation. These colonies