(diatonic semitone), A1 is the augmented unison (chromatic semitone), and S1, S2, S3, S4 are semitones as defined here. In the columns labeled "Interval 1" and "Interval 2", all intervals are presumed to be tuned in just intonation. Notice that the Pythagorean comma (PC) and the syntonic comma (SC) are basic intervals that can be used as yardsticks to define some of the other commas. For instance, the difference between them is a small comma called schisma. A schisma is not audible in many contexts, as its size is narrower than the smallest audible difference between tones (which is around six cents, also known as just-noticeable difference, or JND). Many other commas have been enumerated and named by microtonalists. The syntonic comma has a crucial role in the history of music. It is the amount by which some of the notes produced in Pythagorean tuning were flattened or sharpened to produce just minor and major thirds. In Pythagorean tuning, the only highly consonant intervals were the perfect fifth and its inversion, the perfect fourth. The Pythagorean major third (81:64) and minor third (32:27) were dissonant, and this prevented musicians from freely using triads and chords, forcing them to write music with relatively simple texture. In late Middle Ages, musicians realized that by slightly tempering the pitch of some notes, the Pythagorean thirds could be made consonant. For instance, if you decrease by a syntonic comma (81:80) the frequency of E, C–E (a major third) and E–G (a minor third) become just: C–E is flattened to a justly intonated ratio of and at the same time E–G is sharpened to the just ratio of This led to the creation of a new tuning system, known as quarter-comma meantone, which permitted the full development of music with complex texture, such as polyphonic music, or melodies with instrumental accompaniment. Since then, other tuning systems were developed, and the syntonic comma was used as a reference value to temper the perfect fifths throughout the family of syntonic temperaments, including meantone temperaments. Alternative definitions In quarter-comma meantone, and any kind of meantone temperament tuning system that tempers the fifth to a size smaller than 700 cents, the comma is a diminished second, which can be equivalently defined as the difference between: minor second and augmented unison (also known as diatonic and chromatic semitones), or major second and diminished third, or minor third and augmented second,