of and approved the expedition, and had provided the fleet for the trip. Villegaignon secured his position by making an alliance with the Tamoio and Tupinambá Indians of the region, who were fighting the Portuguese. 1557 Calvinist arrival Unchallenged by the Portuguese, who initially took little notice of his landing, Villegaignon endeavoured to expand the colony by calling for more colonists in 1556. He sent one of his ships, the Grande Roberge, to Honfleur, entrusted with letters to King Henry II, Gaspard de Coligny, and according to some accounts, the Protestant leader John Calvin. After one ship was sent to France to ask for additional support, three ships were financed and prepared by the king of France and put under the command of Sieur De Bois le Comte, a nephew of Villegaignon. They were joined by 14 Calvinists from Geneva, led by Philippe de Corguilleray, including theologians Pierre Richier and Guillaume Chartrier. The new colonists, numbering around 300, included 5 young women to be wed, 10 boys to be trained as translators, as well as 14 Calvinists sent by Calvin, and also Jean de Léry, who would later write an account of the colony. They arrived in March 1557. The relief fleet was composed of: The Petite Roberge, with 80 soldiers and sailors was led by Vice Admiral Sieur De Bois le Comte. The Grande Roberge, with about 120 on board, captained by Sieur de Sainte-Marie dit l'Espine. The Rosée, with about 90 people, led by Captain Rosée. Doctrinal disputes arose between Villegaignon and the Calvinists, especially in relation to the Eucharist, and in October 1557 the Calvinists were banished from Coligny island as a result. They settled among the Tupinamba until January 1558, when some of them managed to return to France by ship together with Jean de Léry, and five others chose to return to Coligny island where three of them were drowned by Villegaignon for refusing to recant. Portuguese intervention In 1560 Mem de Sá, the new Governor-General of Brazil, received from the Portuguese government the command to expel the French. With a fleet of 26 warships and 2,000 soldiers, on 15 March 1560, he attacked and destroyed Fort Coligny within three days, but was unable to drive off their inhabitants and defenders, because they escaped to the mainland with the help of the Tupi Indians, where they continued to live and to work. Admiral Villegaignon had