compatriots returning from the creek, picked them up, and continued into Port Ferdinando. The thirteen survivors were never seen again. Lost Colony Despite the desertion of the Lane colony, Raleigh was persuaded to make another attempt by Hakluyt, Harriot, and White. However, Roanoke Island would no longer be safe for English settlers, following the hostilities between Lane's men and the Secotan, and the death of Wingina. Hakluyt recommended Chesapeake Bay as the site for a new colony, in part because he believed the Pacific coast lay just beyond the explored areas of the Virginia territory. On January 7, 1587, Raleigh approved a corporate charter to found "the Cittie of Raleigh" with White as governor and twelve assistants. Approximately 115 people agreed to join the colony, including White's pregnant daughter Eleanor and her husband Ananias Dare. The colonists were largely middle-class Londoners, perhaps seeking to become landed gentry. Manteo and Towaye, who had left the Lane colony with Drake's fleet, were also brought along. This time, the party included women and children, but no organized military force. The expedition consisted of three ships: The flagship Lion, captained by White with Fernandes as master and pilot, along with a flyboat (under the command of Edward Spicer) and a full-rigged pinnace (commanded by Edward Stafford). The fleet departed on May 8. On July 22, the flagship and pinnace anchored at Croatoan Island. White planned to take forty men aboard the pinnace to Roanoke, where he would consult with the fifteen men stationed there by Grenville, before continuing on to Chesapeake Bay. Once he boarded the pinnace however, a "gentleman" on the flagship representing Fernandes ordered the sailors to leave the colonists on Roanoke. The following morning, White's party located the site of Lane's colony. The fort had been dismantled, while the houses stood vacant and overgrown with melons. There was no sign that Grenville's men had ever been there except for human bones that White believed were the remains of one of them, killed by Native Americans. Following the arrival of the flyboat on July 25, all of the colonists disembarked. Shortly thereafter, colonist George Howe was killed by a native while searching alone for crabs in Albemarle Sound. White dispatched Stafford to re-establish relations with the Croatan, with the help of Manteo. The Croatan described how a coalition of mainland tribes, led by Wanchese, had attacked Grenville's detachment. The colonists attempted to