the reign of Henry II that Huguenot attempts at establishing a colony in Brazil were made, with the short-lived formation of . In June 1559, with war against the Habsburgs concluded, Henri established in letters patent his desire to task much of the Gendarmerie that had been involved in the foreign wars with the extirpation of domestic heresy. Italian War of 1551–1559 The Italian War of 1551–1559 began when Henry declared war on Holy Roman Emperor Charles V with the intent of recapturing Italy and ensuring French, rather than Habsburg, domination of European affairs. Persecution of Protestants at home did not prevent him from becoming allied with German Protestant princes at the Treaty of Chambord in 1552. Simultaneously, the continuation of his father's Franco-Ottoman alliance allowed him to invade the Rhineland while a Franco-Ottoman fleet defended southern France. Although an attempted 1553 invasion of Tuscany ended with defeat at Marciano, in return for his support in the Second Schmalkaldic War, Henry occupied the Three Bishoprics of Toul, Verdun and Metz, acquisitions secured with victory at Renty in 1554. After the abdication of Charles V in 1556, the Habsburg empire was split between his son Philip II of Spain and brother Emperor Ferdinand I. The focus of Henry's conflict with the Habsburgs shifted to Flanders, where Philip, in conjunction with Emmanuel Philibert, Duke of Savoy, defeated the French at the St Quentin. England's entry into the war later that year led to the French capture of Calais, and French armies plundered Spanish possessions in the Low Countries. However, in April 1559 lack of money and increasing domestic religious tensions led Henry to agree the Peace of Cateau-Cambrésis. The Peace was signed between Henry and Elizabeth I of England on 2 April and between Henry and Philip of Spain on 3 April 1559 at Le Cateau-Cambrésis. Under its terms, France restored Piedmont and Savoy to Emmanuel Philibert, but retained Saluzzo, Calais and the Three Bishoprics. The agreement was reinforced by a marriage between Henry's sister Margaret and Emmanuel Philibert, while his daughter Elisabeth of Valois became Philip's third wife. Henry raised the young Mary, Queen of Scots, at his court, hoping to establish a dynastic claim to the Kingdom of Scotland by marrying her to Dauphin Francis on 24 April 1558. Their son would have been King of France and King of Scotland, and also a claimant to the throne of England.