NO· |} Fatty acid β-hydroxylase P450BSβ from Bacillus subtilis (CYP152A1) and fatty acid α-hydroxylase P450SPα from Pseudomonas paucimobilis (CYP152B1) catalyse the hydroxylation reaction of long-chain fatty acids using hydrogen peroxide (H2O2) as an oxidant. These enzymes do not require any reduction system for catalysis. Allene oxide synthase (CYP74A; ), fatty acid hydroperoxide lyase (CYP74B), prostacyclin synthase (CYP8; ) and thromboxane synthase (CYP5; ) are examples of P450 enzymes that do not require a reductase or molecular oxygen for their catalytic activity. Substrates for all these enzymes are fatty acid derivatives containing partially reduced dioxygen (either hydroperoxy or epidioxy groups). References External links Directory of P450-containing Systems