was involved in an experiment that became known as PaJaMo. The PaJaMo experiment, and later work with his student Monica Riley showed that protein synthesis from a gene could begin almost as soon as the gene entered an E.coli cell. Prior hypotheses around the translation of genetic information into proteins had focused on ribosomes, which turned over too slowly to enable the rapid synthesis seen in PaJaMo. On 15 April 1960, Jacob discussed the PaJaMo experiment's findings with Sydney Brenner and Francis Crick at King's College, Cambridge. This caused Brenner and Crick to formulate on the spot the hypothesis that yet another RNA species existed, messenger RNA. Feedback Inhibition With his student Richard Yates, Pardee discovered that biosynthesis of pyrimidine in Escherichia coli is subject to feedback inhibition. This represented an important step in understanding metabolic regulation. Feedback inhibition in glycolysis had been reported by Zacharias Dische in an almost unknown paper a decade earlier. The restriction point In the early 1970s Pardee identified that the cell cycle has a point in the 'G1 phase' where the cell, as it were, 'commits' to moving to the 'S phase'. Pardee published on this so-called 'restriction point', sometimes called the 'Pardee point', in 1974. Students Pardee's students included Allan Wilson, who gained his PhD at Berkeley under Pardee's supervision in 1961. Monica Riley was also a Ph.D. student with Pardee and contributed to his studies of mRNA. References External links The 'PaJaMo' paper from the Journal of Molecular Biology. The 1974 PNAS paper on the Restriction Point. Portrait of an Editorial Board Member: Arthur B. Pardee in the journal Cell Cycle (1:2, 100-100, March/April 2002). U.S. patents that list Arthur Pardee as an inventor. interview: Science in the Blood, BioTechniques, 2006, vol. 41, no. 6, p. 659. A landscape of pinnacles: The extraordinary career of Arthur Pardee Journal of Cellular Physiology. Arthur B. Pardee papers, 1949-2001. H MS c372. Harvard Medical Library, Francis A. Countway Library of Medicine, Boston, Mass. 1921 births 2019 deaths American biochemists Members of the United States National Academy of Sciences Founding members of the World Cultural Council University of California, Berkeley alumni California Institute of Technology alumni University of Wisconsin–Madison faculty University of California, Berkeley faculty Harvard Medical School faculty Members of the American Philosophical Society Members of the National Academy of Medicine