halibut, sardines, beef, chicken, and egg. Vitamins Vitamin A Vitamin A deficiency can lead to rough coat, scaling of skin, and other dermatitis issues like alopecia. It is also essential for cells to properly proliferate keratinocytes, which are epithelial cells that produce keratin on the outermost layer of the skin for the cortisol cells of the hair follicle. A deficiency in vitamin A can cause the common symptoms of dermatitis (dry, scaling skin and dull coat). Vitamin B7 (Biotin) Vitamin B7, also known as biotin, is a water-soluble nutrient that is known to play a role associated with the maintenance and development of hair starting from the follicle. Although it has not been clinically shown to improve hair growth with supplementation alone, it has been shown to reverse deficiency in dogs born deficient. Symptoms of biotin deficiency include alopecia and achromotrichia. A clinical study of biotin showed the importance of biotin in coat pigmentation. There different stages of hair development, as shown in clinical studies using mice. Each stage of hair development has a different sensitivity to biotin present in the body. For example, the shaft development is not greatly affected by biotin access. During the last stage, the amount of biotin available for use by the body will alter the success of that development greatly. Throughout the hair development, most stages of growth are completed, but it was found that in the last stage, where biotin levels were insufficient, there was an incorrect keratinization of the root of the hair, causing the hair to fall out of the body. The supplementation of biotin cannot reverse affects caused by deficiency, but as soon as supplementation is given and biotin levels are restored to adequacy, the body begins to produce the usual hair growth and color it would before the deficiency took place. Essential fatty acids Polyunsaturated fatty acids found in the diet play a critical role in the maintenance of a healthy coat in dogs, and have even been shown to improve coat condition when supplemented in the diet. Furthermore, diets lacking essential fatty acids in their diet will manifest as unkept, matted coat. Omega fatty acids 3 and 6 are highly unsaturated fatty acids, making them especially metabolically active. The proper combination of these omega fatty acids is crucial to achieve optimal benefits. Proper omega 6:3 ratios have been shown to diminish allergy triggered immune responses, thus improving overall