Holsteen was a 60-gun ship of the line in the Royal Dano-Norwegian Navy. She was commissioned in 1775 and the British Royal Navy captured her in the Battle at Copenhagen Roads on 2 April 1801. The British renamed the ship HMS Holstein, and later HMS Nassau. She participated in one major battle during the Gunboat War and was sold in 1814. Design Holsteen was the name ship of her class of three vessels. The Danish naval builder, Frederik Michael Krabbe, was the chief designer and builder for the Danish navy. She was a foot narrower than the otherwise identical Oldenborg-class vessels. Danish service In 1775 Holsteen fitted out during a voyage to Norway, where she was used as a command ship for the ships laid up in Trosvik (near the mouth of the Oslo Fjord), before she returned to Copenhagen in 1776. From 25 May 1776 to 16 July 1780 Holsteen sailed to Lisbon, the Gold Coast, and Cape Town. On her return in July 1780, she performed guardship duties in Øresund, off Copenhagen until 24 October 1780. From 1782 until 1783 Holsteen sailed with the Neutrality Squadron. She was a member of a squadron from June to October 1794 in Øresund and the following year in 1795, sailed with a squadron in Øresund and the North Sea. In 1801 she was equipped as a blockship and took part in the Battle of Copenhagen on 2 April 1801 where she took her place towards the northern end of the defensive line between Infødstretten and Søhesten. For a short time Holsteen served as the flagship. About 14:15 her captain was forced to strike to the British. British service On 12 April, the British sent Holstein back to Britain. She was the only one of the ships-of-the-line that the British chose to keep. All the rest they viewed as little more than floating batteries. She transported the wounded and sailed in company with and , which too carried wounded. She arrived at Yarmouth on 22 April and was laid up there in ordinary until July 1802. Then Holstein transferred to Chatham on 16 October 1802 and was laid up there. Between March and September 1805 Perry & Co., Blackwell, repaired her at a cost of £22,022. She was renamed Nassau and commissioned in September under Captain Robert Campbell, for the North Sea. On 18 April 1806, Nassau was in company with ,