in the image. Noise from other fluorescence compounds can be effectively eliminated by using filters to capture the desired fluorescence wavelength, or by making sure the autofluorescence compounds are not present in the sample. Current and Future Work Modern fluorescence techniques attempt to incorporate methods to eliminate some blurring and noises. Optical aberrations are generally deterministic (it is constant throughout the image process and across different samples). Deterministic blurring can be eliminated by deconvoluting the signal and subtracting the known artifact. The deconvolution technique is simply using an inverse fourier transform to obtain the original fluorescence signal and remove the artifact. Nevertheless, deconvolution has only been shown to work if there is a strong fluorescence signal or when the noise is clearly identified. In addition, deconvolution performs poorly because it does not include statistical information and can not reduce non-deterministic noise such as poissonian noise. To obtain better image resolution and quality, researchers have used statistical techniques to model the probability where photons may be distributed on the detector. This technique, called the Maximum Likelihood method, is being further improved by algorithms to improve its performance speed. References External links Interactive Fluorescence Dye and Filter Database Carl Zeiss Interactive Fluorescence Dye and Filter Database. TIRF Microscopy: Introduction and Applications TIRF Tutorial from Microscopy U TIRF Microscopy: Overview TIRF Tutorial from Olympus Microscopy Resource Center Olympus TIRFM Microscopes commercial TIRF microscope systems Carl Zeiss Laser TIRF 3 commercial TIRF microscope systems Lightguide- and prism-based TIRF microscopy TIRF-Labs.com :Commercial TIRF Microscopy and Spectroscopy. Selecting TIRFM geometry for your application TIRF FLIM microscopy Lambert Instruments TIRF - FLIM microscopy Schwartz Research Group, CU-Boulder Single Molecule Imaging Research Group American inventions Cell imaging Scientific techniques Fluorescence techniques Laboratory equipment Microscopes Optical microscopy techniques