In enzymology, a pyruvate oxidase (CoA-acetylating) () is an enzyme that catalyzes the chemical reaction pyruvate + CoA + O2 acetyl-CoA + CO2 + H2O2 The 3 substrates of this enzyme are pyruvate, CoA, and O2, whereas its 3 products are acetyl-CoA, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. The systematic name of this enzyme class is pyruvate:oxygen 2-oxidoreductase (CoA-acetylating). This enzyme participates in pyruvate metabolism. It employs one cofactor, FAD. References EC 1.2.3 Flavoproteins Enzymes of unknown structure