In enzymology, a phenylglyoxylate dehydrogenase (acylating; ) is an enzyme that catalyzes the chemical reaction phenylglyoxylate + NAD+ + CoA-SH benzoyl-S-CoA + CO2 + NADH The three substrates of this enzyme are phenylglyoxylate, NAD+, and CoA-SH, whereas its 3 products are benzoyl-S-CoA, CO2, and NADH. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is phenylglyoxylate:NAD+ oxidoreductase. It has 3 cofactors: FAD, Thiamin diphosphate, and Iron-sulfur. References EC 1.2.1 NADH-dependent enzymes Flavoproteins Thiamin diphosphate enzymes Iron-sulfur enzymes Enzymes of unknown structure