Anne and the king, who was overjoyed. The following day, Henry wore yellow, a symbol of joy and celebration in England but of mourning in Spain, from head to toe, and celebrated Catherine's death with festivities. With Catherine dead, Anne attempted to make peace with Mary. Mary rebuffed Anne's overtures, perhaps because of rumours circulating that Catherine had been poisoned by Anne or Henry. These began after the discovery during her embalming that Catherine's heart was blackened. Modern medical experts are in agreement that this was not the result of poisoning, but of cancer of the heart, an extremely rare condition which was not understood at the time. Queen Anne, pregnant again, was aware of the dangers if she failed to give birth to a son. With Catherine dead, Henry would be free to marry without any taint of illegality. At this time, Henry began paying court to one of Anne's maids-of-honour, Jane Seymour, and allegedly gave her a locket containing a portrait miniature of himself. While wearing this locket in the presence of Anne, Jane began opening and closing it. Anne responded by ripping the locket off Jane's neck with such force that her fingers bled. Later that month, the king was unhorsed in a tournament and knocked unconscious for two hours, a worrying incident that Anne believed led to her miscarriage five days later. Another possible cause of the miscarriage was an incident in which, upon entering a room, Anne saw Jane Seymour sitting on Henry's lap and flew into a rage. Whatever the cause, on the day that Catherine of Aragon was buried at Peterborough Abbey, Anne miscarried a baby which, according to the imperial ambassador Eustace Chapuys, she had borne for about three and a half months, and which "seemed to be a male child". Chapuys commented "She has miscarried of her saviour." In Chapuys' opinion, this loss was the beginning of the end of the royal marriage. Given Henry's desperate desire for a son, the sequence of Anne's pregnancies has attracted much interest. Mike Ashley speculated that Anne had two stillborn children after Elizabeth's birth and before the male child she miscarried in 1536. Most sources attest only to the birth of Elizabeth in September 1533, a possible miscarriage in the summer of 1534, and the miscarriage of a male child, of almost four months' gestation, in January 1536. As Anne recovered from her miscarriage,