worked on multiple projects at the same time. Florey could be strict with his own collaborators, but gave considerable latitude to those working on other aspects of a project. He did not hold team meetings, although he encouraged team members to discuss issues with himself and each other, and he dropped by each laboratory nearly every day to view progress and provide suggestions. The first such project was an investigation of lymphocytes. Florey developed a detailed project plan and deployed eight graduate researchers on it, including Sanders, Medawar and Taylor. Florey performed delicate surgery on rabbits to examine the effects of lymphocyte deprivation. The project, not completed for many years, resulted in several papers and advances in the understanding of the immune system. Meanwhile, Florey continued with his lysozyme project. Although the Medical Research Council had agreed to pay Roberts's salary, it baulked a providing money for a piece of apparatus that he required. Florey then turned to the Rockefeller Foundation for assistance, and was provided with US$1,250 (). Florey and Magraith harvested lysozyme from animals, and Roberts was able to purify it. Edward Abraham then managed to crystallize it in 1937. Chain and Epstein then studied it and determined that it was a polysaccharidase and, with Gardner's help, were able to determine its structure, and how it acted on polysaccharides. While lysozyme research was successful, it was not fruitful, because while it was lethal to certain bacteria, these were not bacteria that caused illness, and were therefore of negligible concern to medicine. Penicillin Development In the course of his work on lysozyme, Chain read papers on lysozyme in the British Journal of Experimental Pathology by Alexander Fleming in volumes 3 and 8, and by Florey in volume 11. While doing so he found Fleming's paper discussing the antibacterial effects of Penicillium notatum mould in volume 10. The erroneous impression given by Fleming that penicillin was a bacteriologic enzyme led Chain to consider that it would be similar to lysozyme. Money was short at the time; the office had an overdraft of £500 () and Florey had to forbid the purchase of any further equipment whatsoever.Chain and Florey decided to create a large research project on antibacterial substances produced by micro-organisms that could attract long-term funding. Three substances were initially chosen for investigation: Bacillus subtilis, Trueperella pyogenes and penicillin. Florey later said: Florey approached the Medical Research Council in September