children by his third bride; he was the last male Jagiellon in the direct line so the dynasty was threatened with extinction. He sought to remedy this by adultery with two of the most beautiful of his countrywomen, Barbara Giza and Anna Zajączkowska but was unable to impregnate either of them. The Sejm was willing to legitimize, and acknowledge as Sigismund's successor, any male heir who might be born to him; however, the King remained childless. The King's marriage was a matter of great political import to Protestants and Catholics alike. The Polish Protestants hoped that he would divorce and remarry and thus bring about a breach with Rome at the very crisis of the religious struggle in Poland. He was not free to remarry until Queen Catherine's death on 28 February 1572, but he followed her to the grave less than six months later. Health and final years Unlike his father, Sigismund Augustus was more frail and sickly. Shortly before turning 50, his health rapidly declined. Being involved in many affairs and holding a large number of mistresses, historians agree that the king had venereal disease which caused him to be infertile. At 16, he also contracted malaria which further contributed to his inability of producing any offspring. By 1558 Sigismund had gout and since 1568 he also suffered from kidney stones, which triggered immense pain. He employed numerous medics, healers or even quack doctors and imported expensive ointments from Italy. By the end of his life, the king was losing teeth and vigour, possibly due to tuberculosis. Antonio Maria Graziani recalls that Sigismund was unable to keep standing without a cane when greeting Cardinal Giovanni Francesco Commendone. During spring of 1572, Sigismund Augustus became feverish. Untreated tuberculosis made him feeble and impotent, but he was able to travel to his private retreat in Knyszyn. While at Knyszyn, he corresponded with his diplomats and nobles, highlighting that he was feeling well and hoped to recover. Great Marshal Jan Firlej denied these claims and reported that the king was bleeding severely due to consumption and was troubled by pain in the chest and lumbar. Sigismund died in Knyszyn on 7 July 1572 at 6 in the afternoon, surrounded by a group of senators and envoys. The official cause of death given by the medics was consumption. His body placed on a catafalque and remained at the nearby Tykocin Castle until