stain. A knot primer paint or solution (knotting), correctly applied during preparation, may do much to reduce this problem but it is difficult to control completely, especially when using mass-produced kiln-dried timber stocks. Heartwood and sapwood Heartwood (or duramen) is wood that as a result of a naturally occurring chemical transformation has become more resistant to decay. Heartwood formation is a genetically programmed process that occurs spontaneously. Some uncertainty exists as to whether the wood dies during heartwood formation, as it can still chemically react to decay organisms, but only once. The term heartwood derives solely from its position and not from any vital importance to the tree. This is evidenced by the fact that a tree can thrive with its heart completely decayed. Some species begin to form heartwood very early in life, so having only a thin layer of live sapwood, while in others the change comes slowly. Thin sapwood is characteristic of such species as chestnut, black locust, mulberry, osage-orange, and sassafras, while in maple, ash, hickory, hackberry, beech, and pine, thick sapwood is the rule. Some others never form heartwood. Heartwood is often visually distinct from the living sapwood, and can be distinguished in a cross-section where the boundary will tend to follow the growth rings. For example, it is sometimes much darker. However, other processes such as decay or insect invasion can also discolor wood, even in woody plants that do not form heartwood, which may lead to confusion. Sapwood (or alburnum) is the younger, outermost wood; in the growing tree it is living wood, and its principal functions are to conduct water from the roots to the leaves and to store up and give back according to the season the reserves prepared in the leaves. However, by the time they become competent to conduct water, all xylem tracheids and vessels have lost their cytoplasm and the cells are therefore functionally dead. All wood in a tree is first formed as sapwood. The more leaves a tree bears and the more vigorous its growth, the larger the volume of sapwood required. Hence trees making rapid growth in the open have thicker sapwood for their size than trees of the same species growing in dense forests. Sometimes trees (of species that do form heartwood) grown in the open may become of considerable size, or more in diameter, before any heartwood begins to form, for example, in