can distinguish different geometric structures on the same smooth manifold—that is, one can smoothly "flatten out" certain manifolds, but it might require distorting the space and affecting the curvature or volume. Geometric topology Geometric topology is a branch of topology that primarily focuses on low-dimensional manifolds (that is, spaces of dimensions 2, 3, and 4) and their interaction with geometry, but it also includes some higher-dimensional topology. Some examples of topics in geometric topology are orientability, handle decompositions, local flatness, crumpling and the planar and higher-dimensional Schönflies theorem. In high-dimensional topology, characteristic classes are a basic invariant, and surgery theory is a key theory. Low-dimensional topology is strongly geometric, as reflected in the uniformization theorem in 2 dimensions – every surface admits a constant curvature metric; geometrically, it has one of 3 possible geometries: positive curvature/spherical, zero curvature/flat, and negative curvature/hyperbolic – and the geometrization conjecture (now theorem) in 3 dimensions – every 3-manifold can be cut into pieces, each of which has one of eight possible geometries. 2-dimensional topology can be studied as complex geometry in one variable (Riemann surfaces are complex curves) – by the uniformization theorem every conformal class of metrics is equivalent to a unique complex one, and 4-dimensional topology can be studied from the point of view of complex geometry in two variables (complex surfaces), though not every 4-manifold admits a complex structure. Generalizations Occasionally, one needs to use the tools of topology but a "set of points" is not available. In pointless topology one considers instead the lattice of open sets as the basic notion of the theory, while Grothendieck topologies are structures defined on arbitrary categories that allow the definition of sheaves on those categories, and with that the definition of general cohomology theories. Applications Biology Topology has been used to study various biological systems including molecules and nanostructure (e.g., membraneous objects). In particular, circuit topology and knot theory have been extensively applied to classify and compare the topology of folded proteins and nucleic acids. Circuit topology classifies folded molecular chains based on the pairwise arrangement of their intra-chain contacts and chain crossings. Knot theory, a branch of topology, is used in biology to study the effects of certain enzymes on DNA. These enzymes cut, twist, and reconnect the DNA, causing knotting with observable effects such as slower electrophoresis. Topology is also used in evolutionary biology to represent the relationship between phenotype and