Dark. Cloning The Australian Museum in Sydney began a cloning project in 1999. The goal was to use genetic material from specimens taken and preserved in the early 20th century to clone new individuals and restore the species from extinction. Several molecular biologists dismissed the project as a public relations stunt. In late 2002, the researchers had some success as they were able to extract replicable DNA from the specimens. On 15 February 2005, the museum announced that it was stopping the project. In May 2005, the project was restarted by a group of interested universities and a research institute. In August 2022, it was announced that the University of Melbourne will partner with Texas-based biotechnology company Colossal Biosciences to attempt to re-create the thylacine and return it to Tasmania. The university had recently sequenced the genome of a juvenile thylacine specimen and is establishing a thylacine genetic restoration laboratory. DNA sequencing A draft whole genome sequencing of the thylacine was produced by Feigin et al. (2017) using the DNA extracted from an ethanol-preserved pouch young specimen provided by Museums Victoria. The neonatal development of the thylacine was also reconstructed from preserved pouch young specimens from several museum collections. Researchers used the genome to study aspects of the thylacine's evolution and natural history, including the genetic basis of its convergence with canids, clarifying its evolutionary relationships with other marsupials and examining changes in its population size over time. The genomic basis of the convergent evolution between the thylacine and grey wolf was further investigated in 2019, with researchers identifying many non-coding genomic regions displaying accelerated rates of evolution, a test for genetic regions evolving under Positive Selection. In 2021, researchers further identified a link between the convergent skull shapes of the thylacine and wolf, and the previously identified genetic candidates. It was reported that specific groups of skull bones, which develop from a common population of stem cells called neural crest cells, showed strong similarity between the thylacine and wolf and corresponded with the underlying convergent genetic candidates which influence these cells during development. Cultural significance Official usage The thylacine has been used extensively as a symbol of Tasmania. The animal is featured on the official Tasmanian coat of arms. It is used in the official logos for the Tasmanian government and the City of Launceston. It is also used on the University of Tasmania's ceremonial mace and the badge