confirmed anecdotal evidence that A. m. mellifera virgin Queens do not readily mate with non-A. m. mellifera drones, "The progeny of AMM queens was fathered almost exclusively by AMM drones. On the other hand, progeny of AMC queens was fathered by drones of both subspecies". Further research was conducted in western Ireland on Beara peninsula (as part of genetic research carried out throughout the island in 2017), which confirmed the 2013 Polish research in that the A. m. mellifera virgin Queens were not mating with either A. m. carnica or Buckfast drones, nor their hybrids. Several conjectures were presented as an explanation to this characteristic of A. m. mellifera, but no conclusion was reached. Significance The A. m. mellifera had become established from the Urals to northwestern Europe by the 1800's until the introduction of other bee subspecies, considered more suited to modern beekeeping, such as the A. m. carnica or the Buckfast bee, a breed of bee whose ancestry originally included the remnants of the old British black bee (a strain or phenotype of A. m. mellifera), which became extinct due to the Isle of Wight Disease. In the United States, research based on DNA sequencing analysis found DNA from the 'M' lineage of honey bees in the feral population of Arkansas, Louisiana, Mississippi, Oklahoma, and Missouri, believed in part to be the DNA from imported bees of over 100 years ago (DNA from the other bee lineages was also found in these feral populations, suggesting that they likely came from escaped swarms from apiaries at multiple unknown times in the past). Promotion and conservation areas Dedicated organizations have been attempting to establish exclusive conservation areas for A. m. mellifera, also breeding groups have been set up to "establish racial purity" of "native strains" and others running courses to train beekeepers in being able to calculate the "racial purity" of their bees through wing morphometry. Other organizations are attempting to establish that the A. m. mellifera in their local geographic region are a distinct "variety", some even claiming it is a separate subspecies, of the A. m. mellifera subspecies, but to date there is no published research to support this, however through morphometry and DNA analysis local geographic strains may be able to be identified, albeit not consistent across the geographic population, in which the strain's characteristics show less morphometric variation and therefore less environmental adaptability. With one group