can only have happened once. According to some views, prokaryotes lack the cellular architecture to evolve into eukaryotes because a bacterium expanded up to eukaryotic proportions would have tens of thousands of times less energy available to power its metabolism. Two billion years ago, one simple cell incorporated itself into another, multiplied, and evolved into mitochondria that supplied the vast increase in available energy that enabled the evolution of complex eukaryotic life. If this incorporation occurred only once in four billion years or is otherwise unlikely, then life on most planets remains simple. An alternative view is that the evolution of mitochondria was environmentally triggered, and that mitochondria-containing organisms appeared soon after the first traces of atmospheric oxygen. The evolution and persistence of sexual reproduction is another mystery in biology. The purpose of sexual reproduction is unclear, as in many organisms it has a 50% cost (fitness disadvantage) in relation to asexual reproduction. Mating types (types of gametes, according to their compatibility) may have arisen as a result of anisogamy (gamete dimorphism), or the male and female sexes may have evolved before anisogamy. It is also unknown why most sexual organisms use a binary mating system, and why some organisms have gamete dimorphism. Charles Darwin was the first to suggest that sexual selection drives speciation; without it, complex life would probably not have evolved. The right time in evolutionary history While life on Earth is regarded to have spawned relatively early in the planet's history, the evolution from multicellular to intelligent organisms took around 800 million years. Civilizations on Earth have existed for about 12,000 years, and radio communication reaching space has existed for little more than 100 years. Relative to the age of the Solar System (~4.57 Ga) this is a short time, in which extreme climatic variations, super volcanoes, and large meteorite impacts were absent. These events would severely harm intelligent life, as well as life in general. For example, the Permian-Triassic mass extinction, caused by widespread and continuous volcanic eruptions in an area the size of Western Europe, led to the extinction of 95% of known species around 251.2 Ma ago. About 65 million years ago, the Chicxulub impact at the Cretaceous–Paleogene boundary (~65.5 Ma) on the Yucatán peninsula in Mexico led to a mass extinction of the most advanced species at that time. Rare Earth equation The following discussion is adapted from Cramer. The Rare Earth