as cholesterol, except for spongosterol, which is a saturated sterol and more different from cholesterol than the other zoosterols. In plants, the sterols are known as phytosterols. Windaus found that the most common phytosterols are sitosterols, which have the same formula as cholesterol. There are also saturated phytosterols that are mixed in with these unsaturated sitosterols in small quantities. There are also alcohol-like phytosterols, which contain one more hydroxyl group than sitosterols and a different number of carbons. Mycosterols are sterols which are found in fungi. Ergosterol is one significant mycosterol as it has three double bonds, in comparison to the one double bond in cholesterol. In his research, Windaus did not find sterols to exist in bacteria, which he found to be surprising. This research of the composition of sterols, along with their connection to vitamins, earned Windaus the Nobel Prize in Chemistry in 1928. Vitamin D2 and D3 Rickets, a bone disease resulting from vitamin D deficiency, was originally treated in the early 1900s through essential dietary factors such as whole milk or cod-liver oil. It was also postulated that increase sunlight was improving conditions, but was improperly concluded that cholesterol was the precursor activated via UV light. In testing this, investigators under Windaus’ instruction found that completely pure cholesterol - converted into its dibromide and recrystallized - had lost its antirachitic effects upon irradiation. It was then postulated that a different substance associated with ‘chemically pure’ cholesterol through all usual stages of purification is the precursor to vitamin D. The impurity was able to precipitate with digitonin, showing chemical properties of a steroid with three double bonds. The three UV absorption peaks (see image) of the active impurity was used to purify, and become highly concentrated through high-vacuum distillation and charcoal adsorption techniques. In evaluating a variety of sterols that showed antirachitic activity upon irradiation, done so in consultation with A.F. Hess, O. Rosenheim, and T.A. Webster, ergosterol (see image)- Ca27H42 – was found to be the only precursor of vitamin D, convertible under wavelength between 253 and 302 nm. Similarities of ergosterol to that of the active fraction from cholesterol included similar UV spectrums, rapid destruction by oxidation, and production of the same color reaction with sulfuric acid. This production of Vitamin D2, or calciferol (see image), was a complete cure to rachitis – 100,000 times more effective than fish liver oil – and Windaus and