physical barrier. Family groups exist within these wards. Most prairie dog family groups are made up of one adult breeding male, two to three adult females and one to two male offspring and one to two female offspring. Females remain in their natal groups for life and are thus the source of stability in the groups. Males leave their natal groups when they mature to find another family group to defend and breed in. Some family groups contain more breeding females than one male can control, so have more than one breeding adult male in them. Among these multiple-male groups, some may contain males that have friendly relationships, but the majority contain males that have largely antagonistic relationships. In the former, the males tend to be related, while in the latter, they tend not to be related. Two to three groups of females may be controlled by one male. However, among these female groups, there are no friendly relations. The average prairie dog territory takes up . Territories have well-established borders that coincide with physical barriers such as rocks and trees. The resident male of a territory defends it and antagonistic behavior will occur between two males of different families to defend their territories. These interactions may happen 20 times per day and last five minutes. When two prairie dogs encounter each other at the edges of their territories, they will start staring, make bluff charges, flare their tails, chatter their teeth, and sniff each other's perianal scent glands. When fighting, prairie dogs will bite, kick and ram each other. If their competitor is around their size or smaller, the females will participate in fighting. Otherwise, if a competitor is sighted, the females signal for the resident male. Reproduction and parenting Prairie dog copulation occurs in the burrows, and this reduces the risk of interruption by a competing male. They are also at less risk of predation. Behaviors that signal that a female is in estrus include underground consorting, self-licking of genitals, dust-bathing, and late entrances into the burrow at night. The licking of genitals may protect against sexually transmitted diseases and genital infections, while dust-bathing may protect against fleas and other parasites. Prairie dogs also have a mating call which consists of a set of 2 to 25 barks with a 3- to 15-second pause between each one. Females may try to increase their reproduction success by mating with