and a scooping depression to the skull, inflicted by a bladed weapon, most probably a sword. Further, the bottom of the skull presented a gaping hole, where a halberd had cut away and entered it. Forensic pathologist Stuart Hamilton stated that this injury would have left the individual's brain visible, and most certainly would have been the cause of death. Jo Appleby, the osteo-archaeologist who excavated the skeleton, concurred and described the latter as "a mortal battlefield wound in the back of the skull". The base of the skull also presented another fatal wound in which a bladed weapon had been thrust into it, leaving behind a jagged hole. Closer examination of the interior of the skull revealed a mark opposite this wound, showing that the blade penetrated to a depth of . In total, the skeleton presented ten wounds: four minor injuries on the top of the skull, one dagger blow on the cheekbone, one cut on the lower jaw, two fatal injuries on the base of the skull, one cut on a rib bone, and one final wound on the pelvis, most probably inflicted after death. It is generally accepted that postmortem, Richard's naked body was tied to the back of a horse, with his arms slung over one side and his legs and buttocks over the other. This presented a tempting target for onlookers, and the angle of the blow on the pelvis suggests that one of them stabbed Richard's right buttock with substantial force, as the cut extends from the back all the way to the front of the pelvic bone and was most probably an act of humiliation. It is also possible that Richard and his corpse suffered other injuries which left no trace on the skeleton. British historian John Ashdown-Hill had used genealogical research in 2004 to trace matrilineal descendants of Anne of York, Duchess of Exeter, Richard's elder sister. A British-born woman who emigrated to Canada after the Second World War, Joy Ibsen (), was found to be a 16th-generation great-niece of the king in the same direct maternal line. Her mitochondrial DNA was tested and belongs to mitochondrial DNA haplogroup J, which by deduction, should also be the mitochondrial DNA haplogroup of Richard III. Joy Ibsen died in 2008. Her son Michael Ibsen gave a mouth-swab sample to the research team on 24 August 2012. His mitochondrial DNA passed down the direct