the mother of pearl which lines the shell. Natural (or wild) pearls, formed without human intervention, are very rare. Many hundreds of pearl oysters or mussels must be gathered and opened, and thus killed, to find even one wild pearl; for many centuries, this was the only way pearls were obtained, and why pearls fetched such extraordinary prices in the past. Cultured pearls are formed in pearl farms, using human intervention as well as natural processes. One family of nacreous pearl bivalves – the pearl oyster – lives in the sea, while the other – a very different group of bivalves – lives in freshwater; these are the river mussels such as the freshwater pearl mussel. Saltwater pearls can grow in several species of marine pearl oysters in the family Pteriidae. Freshwater pearls grow within certain (but by no means all) species of freshwater mussels in the order Unionida, the families Unionidae and Margaritiferidae. Physical properties The unique luster of pearls depends upon the reflection, refraction, and diffraction of light from the translucent layers. The thinner and more numerous the layers in the pearl, the finer the luster. The iridescence that pearls display is caused by the overlapping of successive layers, which breaks up light falling on the surface. In addition, pearls (especially cultured freshwater pearls) can be dyed yellow, green, blue, brown, pink, purple, or black. The most valuable pearls have a metallic, highly reflective luster. Because pearls are made primarily of calcium carbonate, they can be dissolved in vinegar. Calcium carbonate is susceptible to even a weak acid solution because the crystals react with the acetic acid in the vinegar to form calcium acetate and carbon dioxide. Freshwater and saltwater pearls Freshwater and saltwater pearls may sometimes look quite similar, but they come from different sources. Freshwater pearls form in various species of freshwater mussels, family Unionidae, which live in lakes, rivers, ponds and other bodies of fresh water. These freshwater pearl mussels occur not only in hotter climates, but also in colder more temperate areas such as Scotland (where they are protected under law). Most freshwater cultured pearls sold today come from China. Saltwater pearls grow within pearl oysters, family Pteriidae, which live in oceans. Saltwater pearl oysters are usually cultivated in protected lagoons or volcanic atolls. Creation The mollusk's mantle (protective membrane) deposits layers of calcium carbonate (CaCO3) in the form of the mineral aragonite or