that the mummies at Abusir el-Meleq have 6–15% maternal sub-Saharan DNA while modern Egyptians have a little more sub-Saharan ancestry, 15% to 20%, suggesting some degree of influx after the end of the empire. Other genetic studies show greater levels of sub-Saharan African ancestry in modern southern Egyptian populations and anticipate that mummies from southern Egypt would show greater levels of sub-Saharan African ancestry. Gourdine, Anselin and Keita criticised the methodology of the Scheunemann et al study and argued that the Sub-Saharan "genetic affinities" may be attributed to "early settlers" and "the relevant Sub-Saharan genetic markers" do not correspond with the geography of known trade routes". In 2022, Danielle Candelora noted several limitations with the 2017 Scheunemann et al. study such as its “untested sampling methods, small sample size and problematic comparative data” which she argued had been misused to legitimise racist conceptions of Ancient Egypt with “scientific evidence”. In 2018, the 4000-year-old mummified head of Djehutynakht, a governor in the Middle Kingdom of the 11th or 12th dynasty, was analyzed for mitochondrial DNA. The sequence of the mummy most closely resembles a U5a lineage from sample JK2903, a much more recent 2000-year-old skeleton from the Abusir el-Meleq site in Egypt, although no direct matches to the Djehutynakht sequence have been reported. Haplogroup U5 is also found in modern-day Berbers from the Siwa Oasis in Egypt. A 2008 article by C. Coudray, "The complex and diversified mitochondrial gene pool of Berber populations", recorded haplogroup U5 at 16.7% for the Siwa whereas haplogroup U6 is more common in other Berber populations to the west of Egypt. In 2018, the mummified remains of two high-status Egyptian relatives, Nakht-Ankh and Khnum-Nakht were analyzed DNA by a team of researchers from the University of Manchester. The results found differences in the Y chromosome SNPs of the two mummies which indicated different paternal lineages and concluded that Nakht-Ankh and Khnum-Nakht were half-brothers but Y chromosome sequences were not complete enough to determine paternal haplogroup. The SNP identities were consistent with mtDNA haplogroup M1a1 with 88.05–91.27% degree of confidence, thus confirming the African origins of the two individuals. A 2020 DNA study by Gad, Hawass et al., analysed mitochondrial and Y-chromosomal haplogroups from Tutankhamun's family members of the 18th Dynasty, using comprehensive control procedures to ensure quality results. They found that the Y-chromosome haplogroup of the family was R1b, which originated in Europe and which today