of local warfare on the United States continued until the Anglo-American Jay Treaty went into effect. At the same time, the Spanish also sponsored war within the US by Indian proxies in its Southwest Territory ceded by France to Britain, then Britain to the Americans. Of the European powers with American colonies adjacent to the newly created United States, Spain was most threatened by American independence, and it was correspondingly the most hostile to it. Its territory adjacent to the US was relatively undefended, so Spanish policy developed a combination of initiatives. Spanish soft power diplomatically challenged the British territorial cession west to the Mississippi and the previous northern boundaries of Spanish Florida. It imposed a high tariff on American goods, then blocked American settler access to the port of New Orleans. Spanish hard power extended war alliances and arms to Southwestern Natives to resist American settlement. A former Continental Army General, James Wilkinson settled in Kentucky County, Virginia in 1784, and there he fostered settler secession from Virginia during the Spanish-allied Chickamauga Cherokee war. Beginning in 1787, he received pay as Spanish Agent 13, and subsequently expanded his efforts to persuade American settlers west of the Appalachians to secede from the United States, first in the Washington administration, and later again in the Jefferson administration. Casualties and losses The total loss of life throughout the conflict is largely unknown. As was typical in wars of the era, diseases such as smallpox claimed more lives than battle. Between 1775 and 1782, a smallpox epidemic broke out throughout North America, killing an estimated 130,000 among all its populations during those years. Historian Joseph Ellis suggests that Washington's decision to have his troops inoculated against the disease was one of his most important decisions. Up to 70,000 American Patriots died during active military service. Of these, approximately 6,800 were killed in battle, while at least 17,000 died from disease. The majority of the latter died while prisoners of war of the British, mostly in the prison ships in New York Harbor. The number of Patriots seriously wounded or disabled by the war has been estimated from 8,500 to 25,000. The French suffered 2,112 killed in combat in the United States. The Spanish lost a total of 124 killed and 247 wounded in West Florida. A British report in 1781 puts their total Army deaths at 6,046 in North America (1775–1779). Approximately 7,774