Amazon, noting on his 1707 map that the Marañón "has its source on the southern shore of a lake that is called Lauricocha, near Huánuco." Fritz reasoned that the Marañón is the largest river branch one encounters when journeying upstream, and lies farther to the west than any other tributary of the Amazon. For most of the 18th–19th centuries and into the 20th century, the Marañón was generally considered the source of the Amazon. Scientific exploration Early scientific, zoological, and botanical exploration of the Amazon River and basin took place from the 18th century through the first half of the 19th century. Charles Marie de La Condamine explored the river in 1743. Alexander von Humboldt, 1799–1804 Johann Baptist von Spix and Carl Friedrich Philipp von Martius, 1817–1820 Georg von Langsdorff, 1826–1828 Henry Walter Bates and Alfred Russel Wallace, 1848–1859 Richard Spruce, 1849–1864 Post-colonial exploitation and settlement The Cabanagem revolt (1835–1840) was directed against the white ruling class. It is estimated that from 30% to 40% of the population of Grão-Pará, estimated at 100,000 people, died. The population of the Brazilian portion of the Amazon basin in 1850 was perhaps 300,000, of whom about 175,000 were Europeans and 25,000 were slaves. The Brazilian Amazon's principal commercial city, Pará (now Belém), had from 10,000 to 12,000 inhabitants, including slaves. The town of Manáos, now Manaus, at the mouth of the Rio Negro, had a population between 1,000 and 1,500. All the remaining villages, as far up as Tabatinga, on the Brazilian frontier of Peru, were relatively small. On 6 September 1850, Emperor Pedro II of Brazil sanctioned a law authorizing steam navigation on the Amazon and gave the Viscount of Mauá (Irineu Evangelista de Sousa) the task of putting it into effect. He organised the "Companhia de Navegação e Comércio do Amazonas" in Rio de Janeiro in 1852; in the following year it commenced operations with four small steamers, the Monarca ('Monarch'), the Cametá, the Marajó and the Rio Negro. At first, navigation was principally confined to the main river; and even in 1857 a modification of the government contract only obliged the company to a monthly service between Pará and Manaus, with steamers of 200 tons cargo capacity, a second line to make six round voyages a year between Manaus and Tabatinga, and a third, two trips a month between Pará and Cametá. This was the first step in opening up