The way that the spines are joined at the center of the cell varies and is one of the primary characteristics by which acantharians are classified. The skeletons are made up of either ten diametric or twenty radial spicules. Diametric spicules cross the center of the cell, whereas radial spicules terminate at the center of the cell where they either form a tight or flexible junction depending on species. Acantharians with diametric spicules or loosely attached radial spicules are able to rearrange or shed spicules and form cysts. Holacanthida – 10 diametric spicules, simply crossed, no central junction, capable of encystment Chaunacanthida – 20 radial spicules, loosely attached, capable of encystment Symphiacanthida – 20 radial spicules, tight central junction Arthracanthida – 20 radial spines, tight central junction The morphological classification system roughly agrees with phylogenetic trees based on the alignment of ribosomal RNA genes, although the groups are mostly polyphyletic. Holacanthida seems to have evolved first and includes molecular clades A, B, and D. Chaunacanthida evolved second and includes only one molecular clade, clade C. Arthracanthida and Symphacanthida, which have the most complex skeletons, evolved most recently and constitute molecular clades E and F. Symbiosis Many acantharians, including some in clade B (Holacanthida) and all in clades E & F (Symphiacanthida and Arthracanthida), host single-celled algae within their inner cytoplasm (endoplasm). By participating in this photosymbiosis, acantharians are essentially mixotrophs: they acquire energy through both heterotrophy and autotrophy. The relationship may make it possible for acantharians to be abundant in low-nutrient regions of the oceans and may also provide extra energy necessary to maintain their elaborate strontium sulfate skeletons. It is hypothesized that the acantharians provide the algae with nutrients (N & P) that they acquire by capturing and digesting prey in return for sugar that the algae produces during photosynthesis. It is not known, however, whether the algal symbionts benefit from the relationship or if they are simply being exploited and then digested by the acantharians. Symbiotic Holacanthida acantharians host diverse symbiont assemblages, including several genera of dinoflagellates (Pelagodinium, Heterocapsa, Scrippsiella, Azadinium) and a haptophyte (Chrysochromulina). Clade E & F acantharians have a more specific symbiosis and primarily host symbionts from the haptophyte genus Phaeocystis, although they sometimes also host Chrysochromulina symbionts. Clade F acantharians simultaneously host multiple species and strains of Phaeocystis and their internal symbiont community does not necessarily match the relative availability of potential symbionts in