the vast interior. The success of the venture called attention to the opportunities for economic exploitation of the Amazon, and a second company soon opened commerce on the Madeira, Purús, and Negro; a third established a line between Pará and Manaus, and a fourth found it profitable to navigate some of the smaller streams. In that same period, the Amazonas Company was increasing its fleet. Meanwhile, private individuals were building and running small steam craft of their own on the main river as well as on many of its tributaries. On 31 July 1867, the government of Brazil, constantly pressed by the maritime powers and by the countries encircling the upper Amazon basin, especially Peru, decreed the opening of the Amazon to all countries, but they limited this to certain defined points: Tabatinga – on the Amazon; Cametá – on the Tocantins; Santarém – on the Tapajós; Borba – on the Madeira, and Manaus – on the Rio Negro. The Brazilian decree took effect on 7 September 1867. Thanks in part to the mercantile development associated with steamboat navigation coupled with the internationally driven demand for natural rubber, the Peruvian city of Iquitos became a thriving, cosmopolitan center of commerce. Foreign companies settled in Iquitos, from where they controlled the extraction of rubber. In 1851 Iquitos had a population of 200, and by 1900 its population reached 20,000. In the 1860s, approximately 3,000 tons of rubber were being exported annually, and by 1911 annual exports had grown to 44,000 tons, representing 9.3% of Peru's exports. During the rubber boom it is estimated that diseases brought by immigrants, such as typhus and malaria, killed 40,000 native Amazonians. The first direct foreign trade with Manaus commenced around 1874. Local trade along the river was carried on by the English successors to the Amazonas Company—the Amazon Steam Navigation Company—as well as numerous small steamboats, belonging to companies and firms engaged in the rubber trade, navigating the Negro, Madeira, Purús, and many other tributaries, such as the Marañón, to ports as distant as Nauta, Peru. By the turn of the 20th century, the exports of the Amazon basin were India-rubber, cacao beans, Brazil nuts and a few other products of minor importance, such as pelts and exotic forest produce (resins, barks, woven hammocks, prized bird feathers, live animals) and extracted goods, such as lumber and gold. 20th-century development Since colonial times, the Portuguese portion