Coiled Coil Domain Containing protein 42B, also known as CCDC42B, is a protein encoded by the protein-coding gene CCDC42B. Locus CCDC42B gene is located on the plus strand of chromosome12 at position 24.13 of the long arm. CCDC42B gene starts at 113,587,663 base pairs and end at 113,597,081 base pairs. Part of CCDC42B overlaps with DDX54 gene (113,594,978-113,623,284). The size of CCDC42B is 9,419 bases and its molecular weight is 35,914 Da. CCDC42B mRNA contains 1514 bp and located from 113,587,663 to 113,597,081. CCDC42B protein contains 308 AA and located from 113,587,663 to 113,595,484. The promoter region (GXP_642107) contains 859 bp is predicted to be located from 113,586,906 to 113,587,764. Human CCDC42B gene has three neighbor gene: DDX54, RASAL 1,and DTX1. DDX54 gene is member of DEAD protein family of Putative RNA helicases. The gene encodes DEAD box Protein which has a conserved motif of Asp-Glu-Ala-Asp (DEAD). The DEAD box protein family is associated with cellular processes that involve RNA secondary structure alteration such as RNA splicing, ribosome assembly, Initiation of translation, Nuclear and mitochondrial splicing, Spermatogenesis, embryogenesis, and cell growth and division. The RASAL 1 protein is member of GAP1 family that function in suppressing Ras function by inactivating GDP-bound form of Ras which permit the control of cellular proliferation and differentiation. DTX1 function as ubiquitin ligase protein by facilitating ubiquitination and allowing degradation of MEKK1. The ubiquitin ligase activity of DTX1 regulates the Notch Pathway, a signaling pathway that is associated with cell-cell communications that regulates cell-fate determination. Conservation The Basic Alignment Search Tool (BLAST) of human CCDC42B protein-to-protein database including Mammalia for closely related species, and excluded Mammalia for distantly related species resulted in several orthologs species with reasonable E-value, and high, medium and low coverage depending on the relatedness of orthologs to human CCDC42B. Higher conservation of CCDC42B gene resulted in several strict orthologs (mammalian) of percentage identity range of 95%-53%: rhesus monkey, whale, pig, cattle, and mouse. Lower conservation of CCDC42B gene in distant homologs (non-mammalian) of percentage identity range of 23%-40%: Drosophila, reptile, amphibians and fish. Paralogs CCDC42B gene has only one major paralogs CCDC42'(CCDC42A) Orthologs Human CCDC42B gene is found in ~58 orthologs species. CCDC42B higher conservation in many mammalian orthologs species compared to non-mammalian orthologs species. Higher conservation of CCDC42B gene in several strict orthologs (mammalian): chimpanzee, rhesus monkey, dog,cow, mouse, rat and chicken, and identities that range between 95%-69%. Lower conservation