immunoglobulin C2-set domains. Impact in humans Sialic acid loss Of the 510 identified hCONDELs, only one of these deletions has been shown to remove a 92 bp sequence that is part of a protein-coding region in the human sequence. The deletion that affects the protein coding region in humans results in a frameshift mutation in the CMAH gene which codes for the cytidine monophosphate-N-acetylneurminic acid hydroxylase-like protein, an enzyme involved in the production of N-glycolylneuraminic acid, one type of sialic acid. Sialic acid is known to play a crucial part in cell signaling pathways and interaction processes. The loss of this gene is evident in the undetectable levels of sialic acid in humans but highly present in mouse, pig, chimpanzee and other mammal tissues and may provide more insight into the historic background of human evolution. The mechanisms and time of occurrence of hCONDELs are not entirely understood but given that conserved non-coding sequences play a major developmental role through regulation of genes, their loss in regions of deletions, it is expected that their loss in hCONDELs will result in developmental consequences that can be observed in human-specific traits. In situ hybridization experiments done by Mclean et al. by fusion of mouse constructs fused to basal promoter with LacZ expression for hCONDELs near the androgen receptor (AR) locus and the growth arrest and DNA-damage-inducible protein GADD45 gamma (GADD45G) locus suggest a role in deletions that affect regulatory sequences in humans. Loss of whiskers and penile spine An hCONDEL located near the locus of the androgen receptor (AR) gene may be responsible for the loss of whiskers and penile spines in humans compared to its close relatives, including chimpanzees. The 60.7kb hCONDEL which is located near the AR locus has been found to be responsible for removing a 5 kb sequence that codes for an enhancer for the AR locus. Using the mouse construct with LacZ expression showed localization of this hCONDEL region (AR enhancer) to the mesenchyme of vibrissae follicles and the mesoderm cells of penile organs. Expansion of brain size Many hCONDELs are located around genes expressed during cortical neurogenesis. A 3,181 bp hCONDEL which is located near the GADD45G gene removes a forebrain-specific p300 enhancer binding site. The removal of this region, known to function as a suppressor, specifically increases the proliferation of the subventricular zone (SVZ) of the septum. The loss of this SVZ enhancer region in