cause an accelerated and reversible effect on the synaptic transmission of shi expressing neurons. When shi was tested to cholinergic neurons, the flies showed a quick response to the temperature and were paralyzed within two minutes at 30 degrees, which was reversible. When shi was expressed in photoreceptor cell, fly larva showed temporary temperature-dependent blindness. This experiment shows that shi can be expressed in specific neurons to cause temperature-dependent alterations in behaviours. This research will further be helpful in studying the neuronal subsets in the behaviour of intact animals due to the reversible and controlled manner it is performed in. In more recent studies the Zebrafish ennui mutation was identified from mutagenesis identification for defects in early behaviour. Homozygous ennui embryo swam more slowly than the wild-type but gained normal swimming as it aged. When tested, the motor output of the central nervous system following mechano-sensory stimulation was normal in ennui, which means that the reaction-time and reaction-style were normal to the wild-types and the motoneuron were not affected. The synaptic current at the neuromuscular junction was significantly reduced in ennui which means that the neuromuscular junction was affected. The acetylcholine receptor was significantly reduced in the adult ennui in size as well as localization at the myotome segment borders of fast-twitch muscles. Genetic mosaic analysis revealed that ennui is necessary cell autonomously in muscle fibers for normal synaptic localization of acetylcholine receptors. Also, ennui is very important for agrin function. Ennui is very important in nerve-dependent acetylcholine clustering and the stability of axon growth. In mice, chemical mutagenesis is a phenotype-driven approach to map the mouse mutant catalogue. The usage of mice in behavioural mutation tests allows scientists to increase our understanding of the genetic basis of mammalian behaviour as well as applying this information to human neurological disorders and psychiatric disorders. SHIRPA is a hierarchal screening protocol that efficiently searches for mutations in muscle and lower motor neuron function, Spinocerebellar, sensory neuron function, neuropsychiatry function, and autonomic nervous system function. The mice are then further tested for defects in parameters that are associated with human psychiatric disorders by using two well-known behaviour tests. The mice are tested for locomotor activity (LMA) as well as prepulse inhibition (PPI). For LMA, mice are placed in cages with beam-splitting monitors that will measure the activity of the mice as well as calculate their habituation to that environment. LMA is recorded in