and all axons fail to cross the midline. Functions Slits mediate cell communication in many diverse systems, regulating the guidance, cell migration and polarization of many different cell types. Axon guidance Slit-Robo interactions regulate axon guidance at the midline for commissural, retinal, olfactory, cortical, and precerebellar axons. Deletions of individual robos do not phenotypically match Slit mutants, indicating that Robos1-3 play distinct, complementary but not entirely overlapping roles in axon guidance. In Drosophila, Slit interactions with Robo1 and Robo2 function together in determining whether an axon will cross the midline, and both are necessary for proper crossing. Robo2 and Robo3 function together to specify the lateral position of the axon relative to the midline. The overlapping expression gradients of Robos along longitudinal tracts in the Central Nervous System (CNS) have been referred to as the "Robo-code," but it is unknown whether the formation of specific longitudinal tracts, mediated in this way by Robo, involves Slit signaling. It has been speculated that homophilic and heterophilic binding among Robos may be sufficient to mediate this effect. In vertebrates, Robo1 and Robo2 work together to mediate repulsion from Slit ligands expressed at the floor plate, while Robo3/Rig-1 has the opposite activity, and functions to promote attraction to the midline (most likely by inhibiting the other two Robo receptors, via an unknown mechanism). Mice lacking all three Robos or all three Slits exhibit a phenotype similar to the Drosophila Slit mutant. Axonal and dendritic branching Slit2 and Slit1 have been shown to function as potential positive regulators of axon collateral formation during establishment or remodeling of neural circuits. In fact Slit2-N, an N-terminal fragment of Slit2, has been shown to induce Dorsal Root Ganglion (DRG) elongation and branching, whereas full length Slit2 antagonizes this effect. In central trigeminal sensory axons, however, full length Slit2, through interactions with semaphorin receptor plexin-A4 regulates axonal branching. Interactions between Slit and Robo in this process are unclear, but DRG express Robo2 and trigeminal axons express Robo1-2. Slit-Robo interactions are highly implicated, however, in the dendritic development of cortical neurons in that exposure to Slit1 leads to increased dendritic outgrowth and branching while inhibition of Slit-robo interactions attenuates dendritic branching. Topographic projections Axonal targeting by Slit-Robo appears to play an important role in the organization of topographic projections of axons which correspond to somatosensory receptive fields. In the Drosophila visual system, Slit and Robo prevent mixing of lamaina and