biodegradable scaffolds, ceramics or metal alloys cannot be predicted but must be tested. To meet parameters positively influencing cell adhesion and communication, the technical concept is based on a Minusheet tissue carrier (Fig. 1). By the help of this tool cell adhesion and development of tissue can be tested with individually selected biomaterials. These experiments can be performed first under static (Fig. 2) and then under dynamic (Fig. 3) culture conditions [6]. In both cases a Minusheet tissue carrier prevents damage but supports development of contained cells or tissues during experimentation. To stay compatible with a conventional 24 well culture plate a selected biomaterial must be punched in a diameter of 13 mm. In this format many materials are also commercially available. Further materials can be applied in form of filters, foils, nets, fleeces and scaffolds (Fig. 1a). For an easy handling and to prevent damage during development the selected specimens are placed in the base part of a Minusheet tissue carrier (Fig. 1b). Pressing down a tension ring the biomaterial is held in position (Fig. 1c). After mounting a tissue carrier is enveloped in a bag and sterilized. Cell seeding on a tissue carrier For cell seeding the mounted tissue carrier is transferred by a forceps in a 24 well culture plate (Fig. 2). To concentrate cells on top of a tissue carrier culture medium is added to a level so that the selected biomaterial is just wetted. Then an aliquot of cells is transferred by a pipette to the surface of the mounted biomaterial. A standard culture protocol with a tissue carrier can be initiated by seeding cells onto the upper side. When a tissue carrier is turned, cells can also be seeded on the other side so that co-culture experiments with two different cell types become possible. Not only single cells but also a thin slice of tissue can be mounted between two pieces of a woven net within a Minusheet tissue carrier. Further flexible materials such as collagen sheets can be used in a tissue carrier like the skin of a drum. Last but not least excellent results were obtained by mounting a polyester fleece as an artificial interstitium for spatial parenchyma development [5,6,8]. It is obvious that for each specialized tissue very individual spatial environments within a tissue carrier can be created. Compatible perfusion culture containers It has been shown that the static environment within