seems to increase in the obesity-resistant categories Promoter The promoter region for c8orf33 covers 1191 base pairs of DNA and contains over 700 potential factor binding sites. Fifteen transcription factors with highly conserved binding sites across multiple species’ promoter regions for c8orf33 were selected and shown (see Annotated Promoter Section). CDF1(Cycling DOF Factor 1) physically interacts with FKF1, CDF1 protein is more stable in FKF1 mutants. Another transcription factor, transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II preinitiation complex (PIC). Protein The Isoelectric point of the protein (UPF0488) is 9.16, given a detailed analysis of isoelectric point according to different scales for individual proteins. The Net Charge had been determined using the values available from the Lehninger's Biochemistry book. The precursor protein has a molecular weight of approximately 24.9925 kDa. This is slightly greater than the average pI of 6.81 for the human proteome. It contains repeats from 149 to 166, and 167 to 186. However, the repeats contain a high degree of degeneracy. UPF0488 is an alanine rich protein relative to other proteins and low in all other amino acids besides arginine, leucine, and proline. Homology and evolution The evolutionary lineage of UPF0488 can be traced as distant as invertebrates with a rate of evolution greater than that of fibrinogen. Graph shows divergence of UPF0488 in a given time scale compared to fibrinogen and cytochrome c. Analignment using the SDSC Biology Workbench gives a 27.7% match Danio rerio. The ALIGN calculates a global alignment of two sequences, giving a Global alignment score of 215. The mRNA of UPF0488 has a very high level of degeneracy across organisms. Sequences of very low identity to the human mRNA could only be identified in closely related organisms. However, the protein had far more distant relatives, including several invertebrates. Protein alignments for Homo sapiens UPF0488 was performed using the San Diego Workbench; these alignments were performed against several different taxa including vertebrates such as mammalia, reptilia, aves and invertebrates such as insecta. The protein sequences for UPf0488 are very highly conserved amongst close relatives of homo sapiens such as Gorilla Gorilla Gorilla (Gorilla). The similarity in protein sequence is inversely proportional to divergence (MYA) (table of homologs). Function C8orf33 activity was found to be associated with G protein-coupled receptor signaling pathway, neuroactive ligand-receptor interaction, calcium signaling pathway and the regulation