predicted to undergo N-terminus acetylation, glycation of several lysine residues, SUMOlation, a SUMO interaction at the N-terminus, S-palmitoylation, and extensive phosphorylation. Interacting Proteins BEND2 is found to interact with the following proteins through experimental yeast two-hybrid screens or pull down assays. BEN Domains (protein feature) BEND2 has two BEN domains at its C-terminus. BEN domains are found in a diverse array of proteins and are predicted to be important for chromatin remodeling as well as for the recruitment of chromatin-modifying factors utilized during the process of transcriptional regulation of gene expression. BEN domains are predicted to form four alpha helices that allow this domain to interact with its DNA target. Dai et al. 2013 showed that the Drosophila melanogaster Insensitive (Insv) gene and corresponding protein has no domains of known chemical function yet it contains a single BEN domain. They illustrated the activity of the Insv protein in transcriptional regulation of genes and obtained a crystal structure of two Insv BEN domains interacting with their DNA target site. Expression Tissue expression pattern The expression of the BEND2 gene is regulated and it is therefore not ubiquitously expressed in the human body. High expression occurs in the testis and in the bone marrow. The NCBI EST profile for this gene shows expression only in the testis and in the muscle. Transcriptional regulation of expression The promoter regulating expression of BEND2 (GXP_2567556) is 1255 base pairs in length and is located directly upstream of the BEND2 gene. It regulates transcription of all five transcriptional variants of BEND2. Genomatix's MatInspector program predicted 418 transcription factor binding sites within the BEND2 promoter, including for SRY, neurogenin, interferon regulatory factor-3 (IRF-3), Ikaros2, and TCF/LEF-1. Homology Paralogs The BEND2 protein has no known paralogs within the human genome. BEN-domain containing gene family The BEND2 gene belongs to a family of human genes known as "BEN-domain containing”. This includes BANP (BEND1), BEND3, BEND4, BEND5, BEND6, BEND7, NACC1 (BEND8), and NACC2 (BEND9). The loci for these genes are spread throughout the human genome. Each of these genes contains between one and four BEN domains. Except for at these motifs, the genes of the BEN family do not have similar sequences. Orthologs The BEND2 gene is conserved across evolutionary time as it has 114 known orthologs in a wide range of vertebrate species including mammals, birds, crocodilia, and amphibians. The BEND2 protein has 42 known orthologs. The C-terminus of