LEAPER (Leveraging endogenous ADAR for programmable editing of RNA) is a genetic engineering technique in molecular biology by which RNA can be edited. The technique relies on engineered strands of RNA to recruit native ADAR enzymes to swap out different compounds in RNA. Developed by researchers at Peking University in 2019, the technique, some have claimed, is more efficient than the CRISPR gene editing technique. Initial studies have claimed that editing efiiciencies of up to 80% can be achieved. Synopsis As opposed to DNA gene editing techniques (e.g., using CRISPR-Cas proteins to make modifications directly to a defective gene), LEAPER targets editing messenger RNA (mRNA) for the same gene which is transcribed into a protein. Post-transcriptional RNA modification typically involves the strategy of converting adenosine-to-inosine (A-to-I) since inosine (I) demonstrably mimics guanosine (G) during translation into a protein. A-to-I editing is catalyzed by adenosine deaminase acting on RNA (ADAR) enzymes, whose substrates are double-stranded RNAs. Three human ADAR genes have been identified with ADAR1 (official symbol ADAR) and ADAR2 (ADARB1) proteins developed activity profiles. LEAPER achieves this targeted RNA editing through the use of short engineered ADAR-recruiting RNAs (). consist of endogenous ADAR1 proteins with several RNA binding domains (RBDs) fused with a peptide, CRISPR-Cas13b protein, and a guide RNA (gRNA) between 100 and 150 nt in length for high editing efficiency designed to recruit the chimeric ADAR protein to a target site. This results in a change in which protein is synthesized during translation. History The technique was discovered by a team of researchers at Peking University in Beijing, China. The discovery was announced in the journal Nature Biotechnology in July 2019. Applications Chinese researchers have utilized LEAPER to restore functional enzyme activity in cells from patients with Hurler syndrome. They have claimed that LEAPER could have the potential to treat almost half of all known hereditary disorders. Highly specific editing efficiencies of up to 80% can be achieved when LEAPER editing using arRNA151 is delivered via a plasmid or viral vector or as a synthetic oligonucleotide, though this efficiency varied significantly across cell types. Based on these preliminary results, LEAPER may have the most therapeutic promise with no production of functional protein but if a partial restoration of protein expression would provide therapeutic benefit. For example, in human cells with defective α-L-iduronidase (IDUA) expression in cells from patients with IDUA-defective Hurler syndrome, LEAPER resulted in a W53X truncation