doxorubicin works by observing the genes linked to resistance by using fission yeast as a model system. Links between doxorubicin adverse side-effects and chromosome metabolism and membrane transport were seen. Metabolic models for drug targeting are now being used in biotechnology, and further advances are expected in the future using the fission yeast model system. Experimental approaches Fission yeast is easily accessible, easily grown and manipulated to make mutants, and able to be maintained at either a haploid or diploid state. S. pombe is normally a haploid cell but, when put under stressful conditions, usually nitrogen deficiency, two cells will conjugate to form a diploid that later form four spores within a tetrad ascus. This process is easily visible and observable under any microscope and allows us to look at meiosis in a simpler model system to see how this phenomenon operates. Virtually any genetics experiment or technique can, therefore, be applied to this model system such as: tetrad dissection, mutagens analysis, transformations, and microscopy techniques such as FRAP and FRET. New models, such as Tug-Of-War (gTOW), are also being used to analyze yeast robustness and observe gene expression. Making knock-in and knock-out genes is fairly easy and with the fission yeast's genome being sequenced this task is very accessible and well known. See also DNA damage (naturally occurring) DNA repair Yeast PomBase References External links PomBase - The Pombe Genome Database MicrobeWiki page on Schizosaccharomyces pombe Ascomycota Fungal models Yeasts Fungi described in 1893 ru:Делящиеся дрожжи