diff --git a/data/part_6/0056333083.json b/data/part_6/0056333083.json new file mode 100644 index 0000000000000000000000000000000000000000..33f82a352566c79036f93835b12c104b3fd20e61 --- /dev/null +++ b/data/part_6/0056333083.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"a752054e9fa5d9b21cec3c35fe7606ff","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/f822aafc-d013-42f9-81fa-50a9c57f5600/retrieve","id":"1759592236"},"keywords":[],"sieverID":"b2124891-86fc-4f6c-87f9-9a62592a1ec6","pagecount":"68","content":"Esta publicación no hubiera sido posible sin la participación de las familias que facilitaron la información de sus experiencias y recetas con el proyecto \"Papa, Familia y Clima\" del Ecuador.Agradecemos también el apoyo de las organizaciones de productores Guangaló Tierra Mía, Santa Teresita, La Florida, Agropapa, Chiquicahua, Tambaló, La Dolorosa, UODIC, Fuente de Vida Rumipamba, Ajospamba, Asopropangor y Conpapa. Finalmente, queremos reconocer la contribución de Elena de Jesús Quinga Toasa, que fue clave para el trabajo de investigación en campo.La presente publicación ha sido elaborada con el apoyo financiero de la Unión Europea. Su contenido es responsabilidad exclusiva del Proyecto Regional \"Papa, Familia y Clima. Biodiversidad y buenas prácticas de agricultura climáticamente inteligente para mejorar la resiliencia y productividad de la agricultura familiar en sistemas alimentarios andinos basados en papa\" y no necesariamente refleja los puntos de vista de la Unión Europea.El CIP agradece a los donantes y organizaciones que apoyan globalmente su trabajo, a través de sus contribuciones al Fondo Fiduciario del CGIAR: www.cgiar.org/fundersLos Andes son la región donde la agricultura se practica a la mayor altitud en nuestro planeta. Por ello, los eventos climáticos extremos afectan con una intensidad más pronunciada a la agricultura familiar, campesina e indígena altoandina en comparación con otras agroecologías.Como estrategia de supervivencia, muchos productores de papa han trasladado su producción a las partes más elevadas de las montañas para evitar el impacto de altas temperaturas en el rendimiento y calidad de sus cultivos. En promedio, la papa se cultiva hoy 350 metros más arriba con respecto a 1970. Esto ha generado más emisiones de carbono a la atmósfera, el cual había sido almacenado en los suelos altoandinos intactos por milenios. Así, en una especie de círculo vicioso, la agricultura andina se ubica como uno de los sectores más amenazantes y amenazados por el cambio climático.Para afrontar esta situación, entre junio de 2019 y septiembre de 2021, el Centro Internacional de la Papa (CIP) y el Instituto Interamericano de Cooperación para la Agricultura (IICA) desarrollaron, aplicaron y difundieron prácticas de agricultura climáticamente inteligente (ACI) y articulación a mercados diferenciados para mejorar la capacidad adaptativa de agricultores y actores públicos y privados de los sistemas agroalimentarios andinos basados en papa en Bolivia, Ecuador y Perú.Con una inversión de más de 1.2 millones de euros aportados por CIP, IICA y la Unión Europea, a través del Programa Euroclima+, Componente Producción Resiliente de Alimentos, ejecutado por GIZ y Expertise France, se implementó el proyecto regional \"Papa, Familia y Clima\", que impulsa la biodiversidad y buenas prácticas ACI para mejorar la resiliencia y productividad de la agricultura familiar en sistemas alimentarios andinos basados en papa, en las provincias ecuatorianas de Tungurahua y Chimborazo, en alianza con el Instituto Nacional de Investigaciones Agropecuarias (INIAP), el Ministerio de Agricultura y Ganadería (MAG), 12 organizaciones de productores y otros aliados.Esta publicación busca difundir, como síntesis de logros y lecciones del proyecto, las historias de vida de algunas familias que participaron, así como motivar el consumo de alimentos producidos de forma amigable con el medio ambiente, reconociendo el esfuerzo que implica para las familias campesinas cambiar sus prácticas para aumentar su resiliencia climática. Se llama Euclides por afición de su madre y Asdrúbal por gusto de su padre. Es un hombre de hablar agradable y fluido, apasionado por la agricultura; tiene en su alma ese carisma de aprender con facilidad todo lo que tiene que ver con la tierra. El día que conoció cómo atrapar al gusano blan-Euclides Ocaña, el terror de los gusanos La familia que le ganó la batalla a la plaga co (Premnotrypes vorax) que daña la papa todo cambió para él y su familia.Euclides Asdrúbal Ocaña nació hace 61 años, vive en la parroquia Rumipamba del cantón Quero y pertenece a la Asociación Tierra Mía. En su terreno nacen papas de colores, de PRÁCTICA ACI: Trampas para gusano blanco Euclides Ocaña comparte una tarde con su familia (esposa, hija y hermana), luego de una jornada en el campo.texturas y de sabores únicos. Su afición por este cultivo lo ha llevado, no solo a sembrar la conocida papa chola, sino otras variedades nativas que le han sorprendido por su sabor y su resistencia a la sequía. Él es socio del programa \"Papa, Familia y Clima\", que busca mejorar la resiliencia y productividad de la agricultura familiar.Un grupo de técnicos del CIP, MAG e INIAP, que forma parte de esta iniciativa, le enseñó que hay formas alternativas de atraer al gusano blanco hasta un lugar donde no pueda dañar al tubérculo. El día que se enteró de esto, no solo salvó a sus papas y todo el cultivo, sino que aprendió el comportamiento natural de esta plaga, lo que le permitió planificar sus siembras y usar menos agroquímicos.Sentado en su casa junto a una chimenea, cuenta que antes de aprender sobre la colocación de las trampas, solo conoció al gusano cuando era larva, porque lo veía al final del camino que había hecho, mientras se alimentaba de la papa. Hoy, sabe más de este insecto. Sus ojos se iluminan y relata: \"no solo conozco a la larva que se mete en la papa, sino también a la mamá y al papá, que es más chiquito. Los técnicos me enseñaron que al mes ponen hasta 300 huevos\".Ahora que sabe cómo es el ciclo de vida completo del gusano destructor, se ha dado el trabajo de tomar el tiempo en el que se alimenta de las hojas de papa. Sabe que lo visita a las seis de la tarde, con mucha hambre y sin ninguna contemplación, pero para ese momento Euclides ya tiene listas las herramientas de manejo integrado de plagas para atraparlo.En esa tarea también le ayuda su hija Azucena. Ella toma una botella plástica reciclable, hace dos orificios en la parte alta y adentro coloca una rama de la planta de papa humedecida en un compuesto orgánico, cuyo olor atrae al gusano; luego mete el recipiente en el suelo. La sustancia que usa para atraer al gusano es una mezcla de microorganismos y hierbas que ha sido fermentada previamente en un recipiente.Este no es el único truco que conoce; también tiene otras trampas que le permiten capturar vivo al enemigo para estimar cuándo podría convertirse en un problema grave y de esa forma aplicar insecticidas solo cuando sea absolutamente necesario. Él aprovecha para mostrar su trabajo a los vecinos y que ellos también cultiven sus papas sin molestos inquilinos. Así podrán hacer como Euclides, que cada miércoles vende sus papas en el mercado, confiado en que ningún gusano afectará los ingresos de su familia.Este es un plato típico muy consumido en el lugar. Es habitual en las ferias de los lunes y martes en Ambato, donde lo comercializan de 07:00 a 14:00. Incluso en el cantón Mocha existen carros repartidores que anuncian su venta a través de altoparlantes. La gente del lugar aprovecha para comprarlo durante el almuerzo o llaman por teléfono a los comerciantes para que les entreguen en sus hogares. Generalmente lo venden acompañado de un porción de tostado y un fresco. Cuando se enfríe, pele el grano y lave para retirar cualquier impureza. 4. Licúe el maní hasta el punto de lograr una consistencia granulada. 5. Licúe la cebolla paiteña pelada, lavada y partida en cuatro con media taza de agua. 6. Coloque al fuego la cebolla licuada con la manteca vegetal. Revuelva constantemente hasta que tenga una consistencia pastosa. Añada el maní y mezcle. 7. Coloque dos panes en la licuadora con un cuarto de taza de agua y añada a la mezcla principal. Ponga perejil y sal al gusto. 8. Una vez que esté listo el cuero, retire los excesos de grasa y corte en cuadritos. 9. Para servir: coloque las papas cocinadas, la salsa de maní y el cuero encima. Puede servir con queso alrededor. También se acostumbra acompañar con tostado. 10. Complemente este delicioso plato con ají y adorne con huevo cocinado. Agosto era el mes en el que las heladas llegaban normalmente a la parroquia San Fernando, en el cantón Ambato. De la intensidad del temporal y de los días de duración dependía la magnitud de los daños que podía ocasionar a los cultivos, que se transformaban de un verde intenso a negro. Hasta allí llegaba el deseo de una buena cosecha.Ese día la temperatura caía bajo cero. Y si alguien dejaba una tina de agua a la intemperie se podía apreciar claramente una capa de hielo, como un vidrio fino, que aparecía en la superficie.Ahora, por el cambio climático, no saben cuándo llegarán las heladas. La naturaleza ya no es predecible. Se volvió variable y eso les alerta e intriga.PRÁCTICA ACI: Barreras vivas para proteger los cultivos Alrededor de los cultivos de Raúl Bombón aparecen unas plantas diferentes, no son de papa. Son más altas y están colocadas en los alrededores, como si fueran una cerca. Son hileras de malva, que sirven como barreras vivas para proteger a los sembradíos del viento.Por encima de los 3.000 metros de altura, el aire sopla fuerte y la malva tiene la función de proteger a los cultivos, de evitar que la ventisca vuelva moradas las hojas de la papa y afecte al producto.Este tipo de acciones son parte de lo que se conoce como las buenas prácticas de agricultura climáticamente inteligente (ACI), promovidas por el proyecto \"Papa, Familia y Clima\".En San Isidro de Tambaló, sector donde vive Raúl con su esposa y sus seis hijos, miran a la agricultura de una manera diferente. Comprobaron que fertilizar con abono orgánico fortalece las plantas y mejora el sabor de las papas. \"La comida es más agradable, más arenosa y medio dulce, al momento de servir\", dice este hombre de 47 años, quien combina esta práctica con las barreras vivas de malva como una forma de enfrentar la variabilidad climática.Hace más de tres meses, los Bombón sembraron papa chaucha en su terreno. Desde su casa, Raúl observa que la planta está amarilla y eso significa que la papa está lista para dejar la tierra. Entonces, todos toman sus herramientas: azadones, palas, rastrillos y un balde rojo. Unos cavan, otros sacuden y desde el suelo salen unas papas rojas que contrastan con el negruzco de la tierra. Hoy fue un buen día para esta familia…En la comunidad de Tambaló, en el cantón Ambato, la familia Bombón cosecha la papa chaucha roja.La papa roja a la que no le dio el viento Llegó un poco después de la cosecha de papa. Caminaba sin nombre, su dueña no sabía si era macho o hembra y, cuando descifró este misterio gracias a una vecina, decidió llamarla Josefina. Lleva el nombre de una variedad de papa que se ha vuelto famosa en el lugar, porque es más resistente a los impactos del cambio climático. Algunos creen que la felina salió de la tierra, igual que la papa.Esta gatita ha sido una testigo silenciosa de cómo los abonos verdes incrementaron la productividad de la papa en la familia de Gloria Tibán.Para alimentar a su ganado, Gloria sembró avena y vicia, la primera una gramínea como el maíz, pero sin choclo, y la segunda una leguminosa de gran calidad forrajera. Pero, además de nutrir a los animales, son excelentes abonos verdes que mejoran la calidad de los suelos y permiten a productores como Gloria utilizar menos fertilizantes químicos. Los técnicos agrícolas del programa \"Papa, Familia y Clima\", le recomendaron a Gloria no quemar ni deshacerse de los residuos de la cosecha de avena y vicia, sino incorporarlos al suelo para aumentar el contenido de materia orgánica y mejorar la fertilidad de su terreno de forma natural y más amigable con el medio ambiente. Después de mezclar el suelo con los restos de vicia y avena, lo dejó reposar tres semanas antes de sembrar las semillas de papa.Junto a 11 compañeras de la Asociación de Mujeres Santa Teresita, sembró 5 quintales de papa de la variedad Josefina que le rindieron 44 quintales en la cosecha. Tenía temor de que la producción no fuera buena, pues en mayo y en junio les afectaron las heladas. Sin embargo, gracias a que las plantas crecieron más fuertes y vigorosas por el uso de abonos verdes, el intenso frío solamente dejó unas cuantas hojas quemadas, pero el tallo siguió recto e intacto.Esa es Josefina, la variedad de papa que cuando se combina con prácticas de agricultura climáticamente inteligente resiste mejor a los cambios climáticos, mientras que Josefina, la gata, recorre las plantaciones de papa y sale corriendo cuando llega la helada.PRÁCTICA ACI: Abonos verdes de avena más viciacontra el viento de manera natural.Autora: Gloria Tibán El Runaucho es un plato tradicional del cantón Tisaleo. Los habitantes del lugar refieren que su preparación es común para las grandes fiestas y celebraciones familiares, sobre todo bodas. Tradicionalmente se acostumbra que los priostes de las 40 horas, fiesta en honor al Santísimo Sacramento, ofrezcan este plato a sus más de 200 invitados en el día principal de la fiesta. Esta festividad se celebra antes de la Semana Santa y es un acontecimiento religioso de especial importancia, que convoca a una gran cantidad de personas que participan del arreglo de la iglesia, de oraciones y de la misa central.Ingredientes 1 libra de harina de arveja 2 pepas de ajo macho 3 libras de papa Josefina 1 cuy 1 zanahoria grande 2 ramas de orégano 2 ramas de cebolla blanca 1 cebolla paiteña 1 cucharada de manteca de chancho 1 hoja de apioSal al gusto 1. Hierva dos litros de agua, mientras tanto en una sartén coloque la cebolla blanca, el ajo y el orégano picados con la cucharada de manteca de chancho, sofría y añada al caldo, junto con la zanahoria picada finamente y las papas. 2. En un recipiente con agua fría disuelva la harina de arveja, hasta lograr una mezcla homogénea y ponga en el agua hirviendo. 3. Agregue sal al gusto y remueva constantemente para que no se pegue. 4. Preparación del cuy: Licúe 1 ajo, 1 cebolla paiteña, 1 cebolla blanca, orégano, apio, pimiento y sal al gusto. Condimente el cuy con este preparado, deje macerar por una hora y lleve al carbón para asarlo. 5. Sirva la sopa y coloque una presa de cuy encima. En la casa de Nelly Sánchez hay cinco piedras de moler antiguas. Calculan que la más desgastada puede tener más de 300 años. Nadie las usa, pero no se mueven del patio, son el recuerdo de sus antepasados y de la habilidad que tenían para convertir la cebada, el morocho o el maíz en harina.Esta familia se resiste a olvidar los saberes ancestrales de los Andes. Por eso el día en que Luis Ernesto Sánchez, esposo de Nelly, encontró por casualidad a un vecino que resguardaba la papa cacho, una variedad nativa de color negro que tiene la forma de cornamentas de toro, no dudó en recuperar esta semilla. En su familia la habían dejado de ver hace mucho tiempo, pero les traía recuerdos de su niñez y siempre añoraban su sabor. Igual nombran con nostalgia las papas tabaquera, jardinera, lima, que forman parte de las 350 variedades de papas nativas registradas en el Ecuador.Así, con las pocas semillas de papa cacho que su vecino, custodio de la agrobiodiversidad, les regaló, sembraron tres guachos, pero al principio cultivaron unos tubérculos muy pequeños. Porfiado en que no se podía perder el patrimonio natural y cultural del país, Luis Ernesto las sembró en otro lugar y allí crecieron grandes, relucientes, con una curva que hasta parecía sonrisa. Ese día empezaron a recuperar la semilla ancestral. De eso, hace dos años.Se dieron cuenta que tuvieron mejores resultados porque el segundo terreno estaba fertilizado orgánicamente con productos naturales.Este hogar también forma parte del programa \"Papa, Familia y Clima\". Y, además de revalorar a las papas nativas, también están aplicando otra práctica de agricultura climáticamente inteligente (ACI) que reaprendieron en las capacitaciones del CIP, MAG e INIAP: el uso de plantas repelentes para ahuyentar a las plagas de forma natural, sin la necesidad de aplicar tantos insecticidas químicos. \"Cuando íbamos a cosechar la planta, no había papas. Hoy, no nos ha ganado la plaga y estamos cuidando nuestro bolsillo y al medio ambiente\", cuenta Nelly Sánchez.Ella recorre sus cultivos y observa que están verdes y sanos. Alrededor de ellos, como si fuera una hilera, se observan tallos de cebolla blanca a cincuenta centímetros de distancia. Más allá aparece una barrera de apio, que también ahuyenta a mosquitos que tienen bien identificados los Sánchez-Sánchez.El día que perdieron sus cosechas tomaron una lupa y vieron a los insectos de cerca. Querían conocer a detalle a esta plaga para estar mejor preparados. Ahora, ya no les cogerá de sorpresa porque están armados con \"barreras vivas\" y bien capacitados.Plantas repelentes, las mejores amigas de la papa PRÁCTICA ACI: Siembra de plantas repelentes Autora: Nelly Sánchez Este es un potaje de autoría de la familia Sánchez, que buscó una forma novedosa de presentar la papa cacho. La madre de este hogar decidió armar una combinación que resalte el sabor de esta papa andina y consideró que la salsa de maní y la pata de res serían una buena opción. También recomienda que se acompañe el platillo con un delicioso jugo de mora, que es otro de los cultivos populares de la zona.Papa cacho en salsa de maní con pata de res PRÁCTICA ACI: Asociación de cultivosIntegrantes de la Asociación Carihuairazo de Chiquicahua posan en una hacienda antigua, que ahora es su sede.pero que acompaña siempre a los paisajes altoandinos.Los 20 quintales de papa se sembraron con prácticas de Agricultura Climáticamente Inteligente (ACI) promovidas por el proyecto \"Papa, Familia y Clima\". Antes de depositar las semillas en la tierra, llegó un camión lleno de abono orgánico que dejó el suelo como una cobija suave y esponjosa enriquecida con nutrientes naturales.Ese día, José Manuel Pilamunga se sintió muy complacido pues esta práctica pone en valor los saberes ancestrales de la agricultura andina y los fortalece con la ciencia del CIP y el INIAP. Lleva un poncho rojo que le llega más allá de la cintura. Casi no habla, solo asiente, pero cuando pronuncia una frase es precisa y reveladora.Al escuchar a los compañeros de la comunidad comentar sobre el cultivo con abonos orgánicos, alza un tanto la voz para recordar: \"Así mismo hacíamos antes y las papas nos duraban hasta un año, guardábamos en el soberado (bodega ubicada en la parte alta de la casa, antes del tejado). Con tanto químico la tierra se cansa, hay que volver a lo orgánico. Mi mamita, sin químico, vive hasta ahora y tiene casi 100 años\".Esta comunidad no solo volvió a utilizar fertilizantes orgánicos, sino a la práctica agroecológica de asociación de cultivos. En los terrenos de la comunidad se puede ver papa en una hilera y haba en la otra. Aprendieron la importancia de esta combinación para mejorar los suelos y obtener rendimientos estables en las capacitaciones de EURO-CLIMA+, que conjugaron los saberes locales con las últimas innovaciones basadas en la ciencia.Damián Punina, uno de los más jóvenes de la comunidad, entusiasta por implementar estas prácticas, quiere mirar a la tierra con los ojos de sus abuelos. Y dice que hay que entender que los cultivos se ayudan entre sí. \"Si se siembra papa y chocho no se perderá el lote de la siembra, porque el chocho es una planta agria que evita que lleguen las plagas, los moscos y protege a la papa de las heladas al ser una plata alta\".Los 123 socios de esta asociación esperan ansiosos la cosecha. Están contentos con el esfuerzo que hacen en la producción cada vez más amigable con el ambiente y con papas más saludables y nutritivas para la mesa de las familias.Cuando las mujeres del lugar dan a luz es habitual que se prepare este platillo, con el propósito que recuperen fuerzas durante los primeros 15 días. La receta inicialmente incluye solo cuy y cebolla; solo después de transcurrido este tiempo se puede añadir papas y otros elementos al preparado. El cuy es parte de la dieta central luego del parto pues existe la creencia que ayuda a producir leche, al igual que la papa que proporciona nutrientes. El sábado llegó con viento y baja temperatura a Mulanleo, en la parroquia Pilahuín, en Ambato. Allí el clima hace temblar de frío a los extraños. En ese lugar, las papas nativas tienen un espacio especial desde que los miembros de la Asociación Agropapa decidieron recuperar la agrobiodiversidad andina.Son papas deliciosas y adaptables a cualquier preparación. Se pueden cocinar, hacer puré o freír. Mientras más oscuro es su color de piel o pulpa, mayor es el contenido de antioxidantes.Por los alrededores se pueden ver plantaciones de Yana Shungo, que significa corazón negro. Son más largas que anchas, tienen ojos profundos y el color de la corteza es de un marrón intenso. Cuando se la corta, la pulpa es morada con un anillo de color crema.También está la Puca Shungo. Si se traduce del quichua al español significa corazón rojo, por el color predominante de la pulpa. El trío de estas papas nativas lo completa la Chumbi, de color rojizo.El día que salieron al mercado con estas papas y no hubo interés en los compradores, la creatividad de losagricultores se puso a prueba y buscaron la forma de volverlas atractivas. Así, nació \"Yapu Chips\", una golosina que combina estas variedades nativas en hojuelas altamente nutritivas. Ahora, ya tienen su planta de industrialización propia, que se levantó con el apoyo del CIP y el MAG. Luego, con el apoyo del IICA dentro del proyecto \"Papa, Familia y Clima\", se innovó la comercialización de la organización al diseñar la marca AGROPAPA, su logotipo y una mejor estrategia para la venta de las Yapu y de otros productos que tienenPRÁCTICA ACI: Innovaciones comerciales tritis, por las cualidades especiales que tienen las papas nativas.Paulina López, ingeniera agrónoma de profesión, vio la potencialidad de este producto en el tema culinario y decidió estudiar un curso de cocina andina para crear sus propias recetas, inspiradas en las papas de colores. Su familia, que forma parte de la Asociación Agropapa, es una apasionada de estas variedades únicas del Ecuador. Las comen de día, de noche, en sopa, en puré, fritas y hasta en llapingachos de colores.las familias de Agropapa. Ahora se los encuentran en varios mercados locales y regionales, y pronto esperan estar en las grandes ciudades del país.El ingenio no paró allí. También se creó el cóctel de papas nativas, una bebida hecha con una pequeña cantidad de licor y pulpa de guanábana y desde luego las deliciosas papas que le dan un atractivo color a estas bebidas. Es una mezcla que logra un sabor único y exótico. Aseguran que sirve no solo para amenizar los encuentros sociales, sino para curar la gas-La familia Montesdeoca-López exhibe todos los productos que fueron inspirados con las papas nativas.El llapingacho, palabra quichua que quiere decir papa aplastada, es un plato que aparece luego del terremoto de 1949, en Ambato. Generalmente, se prepara con papa chola y se añade achiote y queso. Sin embargo, en Mulanleo han propuesto una variación, la cual consiste en mantener los ingredientes habituales, pero usar papas nativas para su preparación, con lo que dan más vistosidad al plato, ya que cada tortilla tiene un color diferente que va desde el morado hasta el amarillo pálido. Además, aprovechan los cultivos de la zona y dan valor a las papas nativas. Las tres variedades de papa se cocinan por separado, con sal y cebolla según el tiempo de cocción de cada una. 2. Una vez cocidas, aplaste cada papa por separado con un poco de mantequilla. Haga tortillas y fría. 3. Cocine la remolacha y corte en cuadritos. Añada sal, aceite y limón. 4. Cocine el chorizo por unos tres minutos en agua hirviendo y luego proceda a freírlo. 5. Fría los huevos 6. Sirva las tortillas con el chorizo, el huevo y la ensalada de remolacha sobre una sábana de lechuga. Añada el tomate cortado en cuadros y el aguacate en tajadas y la cebolla colorada curtida. 7. Opcional: si desea puede acompañar el plato de alguna salsa. Para ello, pique cebolla blanca y haga un refrito con achiote, dos dientes de ajo y sal. Aparte, licúe un pan con una taza de leche y agregue al refrito. Hierva hasta que espese y añada al plato principal.La lucha contra el mosco que produce la enfermedad de la punta morada punta morada de la papa, que puede causar la pérdida total del cultivo en poco tiempo. Por si esto fuera poco, este insecto también puede transmitir otras enfermedades como \"cebra chip\" o \"papa rayada\".A esta plaga no le atraen solo las papas, tiene una fascinación por el color amarillo. Cuando lo ve, vuela hipnotizada hacia él. Es su perdición.Le conocen como Bactericera cockerelli. Tiene alas transparentes y el color de su cuerpo varía entre amarillo, café oscuro o negro. Vuela por el campo buscando cultivos de papa y otras solanáceas, como el tomate y los ajíes. Es un ser sediento que, cuando llega a la planta, extrae la savia provocando que una parte de sus hojas se torne violeta, como si tuviera una hemorragia. Es la temible PRÁCTICA ACI: Trampas para bactericera Hasta hace un tiempo era un enemigo invencible, cuya llegada producía que la planta de la papa no crezca y que los tubérculos nazcan en los tallos, en lugar de la tierra. Es decir, crecían papas aéreas, que no se desarrollaban.Afortunadamente, esa es una historia que ya no se repetirá en las plantaciones de las mujeres de la Asociación La Dolorosa. Interesadas en garantizar su cosecha, formaron parte de las capacitaciones del proyecto \"Papa, Familia y Clima\", que les mostró prácticas de manejo integrado de plagas, como el uso de trampas amarillas para Bactericera y el software ILCYM del CIP, que les permite identificar qué zonas son más propensas a los daños de esta plaga a medida que el clima va cambiando.Una de las más entusiastas es Yamari Flores. Ella viene de un sitio donde se consume más yuca o plátano. Oriunda de Shushufindi, en la Amazonía, llegó al cantón Tisaleo, parroquia La Matriz, hace un año y medio. Poco a poco la papa la sedujo. En su casa reservó un espacio de terreno para su primer sembrío, pero antes quiere aprender todo y saber cómo cuidar y defender a su cultivo.Casi son las seis de la tarde y un grupo de 7 mujeres extiende sobre los sembríos de papa un plástico amarillo untado con aceite. El trabajo da buenos resultados pues atrapan a muchos insectos. Luego los cuentan para estimar la cantidad de bichos por temporadas y de esa forma saber el momento preciso en que vale la pena implementar medidas de control de plagas, cuidando su bolsillo, el medio ambiente y, lo más importante, la salud de ellas, sus familias y de quienes consuman las papas que cosechen. Yamari se siente más preparada para sembrar y para sorprender a sus compañeras con una papa tan grande como una yuca.Un grupo de mujeres unta con aceite vegetal un plástico amarillo para atrapar al insecto que enferma a la papa.Esta es una combinación de granos, cuyo ingrediente principal son las papas nativas que se siembran en el cantón Tisaleo. Es común que los habitantes de este lugar lleven este plato al campo cuando realizan trabajos en conjunto, lo que popularmente se conoce como la minga. Se sirve de manera comunitaria, es decir se coloca un mantel en el suelo y se riega toda la comida que se va a compartir. A esta práctica se llama Pambamesa. Manuel Pucha, uno de los pobladores más antiguos de La Pradera en el cantón Colta, Chimborazo, estuvo presente el día en que la luz eléctrica llegó a la comunidad, hace casi 30 años.También fue testigo del momento en que niñas y niños dejaron de ir al pozo a cargar agua porque llegó el agua potable. De la misma manera, Manuel acompañó al proyecto \"Papa, Familia y Clima\" desde que inició actividades en La Pradera, participando activamente en las capacitaciones del CIP, MAG e INIAP para elaborar un abono orgánico con el cual las matas producen más y mejores papas.Cada vez que la comunidad se reúne para preparar este compuesto, conocido como compost, él llega con cuaderno y esfero en mano para anotar la receta exacta de la mezcla perfecta: melaza, ortiga, marco, chilca y estiércol. Observa con detalle la cantidad que se debe añadir de roca fosfórica, un elemento natural que nutre las plantas al aportarles calcio y fósforo.Con sus ojos expresivos, mira atento cuando Manuel Parco, líder de la comunidad, dirige la elaboración del fertilizante orgánico. Tiene muchas historias por escribir sobre el cultivo de papa, pero su cuaderno lo utiliza exclusivamente para registrar el trabajo que vienen realizando en cuanto a la agricultura climáticamente inteligente (ACI), que ayuda al manejo integral de El abono orgánico se hace en minga los sistemas agroalimentarios altoandinos para responder de forma eficaz al cambio climático. Su impresión es que los resultados de EUROCLIMA+ en su comunidad son buenos porque en la última cosecha obtuvieron 50 sacos de papas. Antes, solo conseguían 30.Allí todo se hace en comunidad. Las personas que integran la Asociación Comuna La Pradera siembran juntas, construyen caminos juntas y cosechan juntas los frutos de su trabajo. Se consideran como una gran familia y comparten historias en común, como la preparación de compost y biol.En un espacio de a p ro x i m a d a m e n t e veinte metros cuadrados se van mezclando uno a uno los ingredientes, que deberán reposar por dos meses antes que el abono esté listo para usarse en los terrenos de las 30 familias que viven en el lugar. El cultivo actual de papa ha soportado ya tres heladas, pero sigue en pie. La comunidad atribuye esta mejora en su resiliencia, a que ahora las plantas están más fuertes por el uso de biol y otras prácticas ACI, como el uso de semillas más resistentes a estos fenómenos.Si los efectos del cambio climático son impredecibles, la comunidad es ingeniosa… PRÁCTICA ACI: Elaboración de abonos orgánicos (compost y biol) La sopa de quinua con cuy es un platillo muy consumido en la comunidad La Pradera. Es una tradición heredada de generación en generación y se prepara con productos cosechados por los habitantes del lugar. Los pobladores refieren que el secreto del sabor es la cocción en leña y el correcto lavado del grano para quitar el sabor amargo. Esta sopa puede hacerse también con carne de cerdo, pero es más común el uso del cuy.Ingredientes 1 1 / 2 libra de quinua 2 libras de papa 1 cuy 1 rama de cebolla blanca 1 cebolla paiteña 1 zanahoria 2 ajos 1 rama de apio 2 ramas de cilantro 1 rama de perejil 1 taza de leche Sal y achiote al gusto 1. Lave bien la quinua hasta que el grano quede completamente limpio. 2. Coloque cinco litros de agua en una olla y cuando hierva ponga la quinua. 3. Cocine por media hora o hasta que el grano esté suave. Luego, añada las papas peladas, el cuy en presas y la zanahoria picada. 4. Agregue la cebolla blanca, la paiteña y el ajo, finamente picados. 5. Coloque el cilantro y el perejil picados. 6. Como paso final, ponga achiote y una taza de leche antes de servir.En la comunidad Lig-Lig hay una vistosa laguna a la que llegaban unas aves pequeñas de color café oscuro con pecho blanco. A su paso hacían un sonido como lig, lig, lig, lig... Ahora llegan pocas, de vez en cuando, pero dejaron el recuerdo de su nombre. Esta es la historia que narran en este lugar del cantón Colta en la provincia de Chimborazo.Allí se cuentan relatos que no se pueden creer, por lo impresionantes. Uno de ellos es el de una variedad de papa que parece chola, pero que se cosecha tres meses antes de lo acostumbrado.El personaje principal tiene el nombre de una mujer resiliente: la papa Josefina, que requiere menos agua para crecer, tolera algunas enfermedades como el tizón tardío o lancha, que al PRÁCTICA ACI: Uso de variedades adecuadas de papa consumirla nos aporta cantidades importantes de vitamina C, hierro y caroteno. De esto hay evidencias científicas en el INIAP y el CIP.Josefina es una variedad de papa que no le teme a las alturas, que entre los 2.700 y los 3.500 metros sobre el nivel del mar está lista para cosechar en 4 o 5 meses, aproximadamente. En la región andina del Ecuador soporta sequías, enfermedades y hasta las temibles heladas.Alicia Naula quedó impresionada cuando vio que de cada planta de la variedad Josefina salían entre 25 y 30 papas. En las mejores cosechas de otra variedad cultivaban solamente 15 tubérculos por mata, si es que los efectos del cambio climático y las plagas no acababan antes con el cultivo.La correcta elección de semillas, adaptadas al entorno, es lo que se conoce como uso adecuado de variedades de papa y que forma parte de las prácticas de Agricultura Climáticamente Inteligente (ACI) promovidas por el proyecto \"Papa, Familia y Clima\". Aplicar esta medida permitió que la comunidad, en su última cosecha, obtenga 150 sacos del producto, cuando antes sólo obtenían 60 sacos.Desde ese día, Josefina es una de las principales aliadas de las 33 familias de esta comunidad.Día especial en la comunidad Lig Lig. Los cultivos de papa Josefina están listos para la cosecha.Este platillo, cuyo ingrediente principal es el achiote, surge del ingenio de los abuelos y abuelas del lugar para lograr una combinación que potencie el sabor de la papa. Esto en vista de que eran escasos otros ingredientes para hacer salsas que acompañen al tubérculo, que se cultiva permanentemente en la zona y que es un elemento central en la dieta de las familias. El secreto para lograr un sabor diferente y un toque ahumado es la cocción en leña, así como la colocación de tallos de quinua y haba en el fuego. Esto otorga un sabor diferente, tanto a las papas como al cuy asado.Ingredientes 5 libras de papa 3 cucharaditas de achiote 2 ramas de cebolla 3 pepas de ajo 2 cuyes Sal al gusto 1. Ponga a cocinar la papa cuando hierva el agua. 2. Ase el ajo y la cebolla en el fogón y triture en la piedra de moler junto con el achiote, hasta que la mezcla se vuelva homogénea. 3. Añada tres cucharadas del caldo donde se cocinó la papa. 4. Coloque la mezcla en las papas cocinadas, recién sacadas del fuego. Es importante que se mantenga el calor de las papas para que el achiote se cocine ligeramente y se obtenga el sabor característico de este plato. 5. Para preparar el cuy: sazónelo con ajo y sal. En la barriga y en la boca del animal coloque ajo y cebolla. Ase en el carbón hasta el punto deseado. 6. Para servir: ponga las papas y encima el cuy. Puede acompañar con un vaso de chicha y con ají. Igual que un ejército, cada uno tiene habilidades especiales, que van desde la destreza para disolver los micronutrientes del suelo, para que la papa pueda asimilarlos fácilmente, hasta la gracia para encontrar a los insectos que causan daño a la planta y parasitarlos.En el teléfono celular de César Asaquibay, ingeniero agrónomo del Instituto Nacional de Investigaciones Agropecuarias (INIAP), hay evidencia de esto. Él tomó una foto a una de las contrincantes más feroces de la papa: la mamá del gusano blanco. La encontró cuando caminaba cerca de las papas, cubierta de microorganismos benéficos que simulaban ser bolas de algodón y que la destruyeron.En otra parcela del señor Sadva, los microorganismos benéficos perdieron la batalla, no por falta de fuerza ni de talento. Un terreno desfavorable produjo que la planta empiece a podrirse. Se tomaron varias muestras de este suelo para un análisis específico en laboratorio y ver cuál es la causa exacta. Mientras tanto, un nuevo batallón de microorganismos benéficos espera para entrar a combatir y recuperar el terreno.Seres microscópicos que mejoran el terreno PRÁCTICA ACI: Utilización de microorganismos para el suelo Es el plato preferido para servirse en grandes compromisos sociales. Los pobladores de Chimborazo refieren que su consumo se ha incrementado debido a la creencia de que su carne es beneficiosa para combatir el coronavirus. Es usual que se sirva en mercados y restaurantes.Ingredientes 1 cuy pelado 4 dientes de ajo machacados 1 cucharada de comino ½ cucharadita de achiote 2 kilos de papas peladas 2 cucharadas de aceite 2 ramitas de cebolla finamente cortadas 2 cebollas coloradas finamente picadas 1 pizca de orégano 1 pizca de pimienta molida 1 cucharada de cilantro ½ litro de leche ½ libra de maní 1 lechuga pequeña Sal al gusto 1. Cocine las papas 2. Muela en la piedra 2 ajos, 2 cebollas coloradas y 1 rama de cebolla blanca. Añada comino, achiote, pimienta molida y sal. Aliñe el cuy con este preparado y deje reposar por una hora. Ase en el fuego, o al horno si lo desea. 3. Tueste el maní, pele y retire las impurezas, licúe con media taza de leche y reserve. 4. Haga un refrito con 2 ajos, 1 cebolla colorada, 1 rama de cebolla blanca y cilantro finamente picados. Añada la mezcla de maní y hierva hasta que espese, coloque sal al gusto. Adicione una pizca de orégano al final. 5. Para servir, ponga la lechuga debajo de las papas y riegue la salsa de maní alrededor del tubérculo. Coloque el cuy encima de este preparado.En la vía Panamericana, en el sector Rumipamba del cantón Colta, el cruce de vehículos es muy cotidiano. En ese lugar se ubica una tienda comunal y una construcción rústica hecha de madera y paja, que se activa en tiempo de cosecha para vender las papas frescas. Así, la arenosa papa chaucha amarilla llega a varios destinos en los autos de los compradores: Guayaquil, Milagro, Machala y otros sitios.Cerca de ahí, bajando por caminos inclinados se llega a un gran terreno, cuya siembra pertenece a 12 familias de la Asociación Fuente de Vida Rumipamba. Bajo tierra, están creciendo dos variedades de papa: Josefina y Fripapa, con las cuales los agricultores están aprendiendo a usar un juego de ruedas diseñado por el CIP, que les ayuda a tomar decisiones informadas sobre si es necesario fumigar y cuándo hacerlo. De esta forma evitan el uso excesivo de agroquímicos, protegen el suelo y cuidan a sus plantas de la lancha, una enfermedad que afecta al follaje y destruye a la papa. Se presenta cuando el tiempo es húmedo, hay niebla, frío y luego temperaturas altas.El juego de ruedas es una herramienta circular que los miembros de la Asociación Fuente de Vida Rumipamba recibieron en el marco del programa \"Papa, Familia y Clima\", que llevan adelante el CIP y el IICA en alianza con el MAG y el INIAP y el apoyo de la Unión Europea.Elena Quinga, agrónoma del CIP, llega a sus visitas de acompañamiento técnico en campo -alegre y preparada, como siempre. En su mochila tiene todas estas herramientas. Son tan vistosas que parece que va a una celebración del Inti Raymi.Con su mano derecha sostiene una pila de tarrinas pequeñas transparentes, con bordes de colores: amarillo, rojo y verde, los mismos del semáforo. Las entregan a los agricultores para que las coloquen en la tierra y puedan medir la lluvia que cae, dato indispensable para mover las ruedas.De acuerdo con la variedad de papa, la cantidad de lluvia y la última fecha de fumigación, se obtienen datos que, al sumarlos, revelan cuándo se debe fumigar y qué tipo de producto usar, cuidando siempre la alternabilidad para que la tierra no se acostumbre y pierda efecto.De esta forma, las familias campesinas protegen con esmero la nueva papa que ofertarán al filo de la carretera, con la historia de que fue cosechada usando técnicas de la Agricultura Climáticamente Inteligente (ACI) y que, por lo tanto, son más saludables y amigables con el medio ambiente. Esta es una historia que apenas comienza…Nuevas herramientas para proteger los cultivos de papa PRÁCTICA ACI: Sistema de Apoyo a la Decision (SAD) para el control de lancha La última vez que José Guamán levantó su mirada para ver volar a un cóndor tenía 15 años, de eso ya hace seis décadas. Sentado frente al fuego, junto con su esposa Doralitza Fernández, se abriga con el calor que produce la leña y recuerda que en la parroquia Juan de Velasco Pangor, en el cantón Colta en Chimborazo, los veranos ya no son como antes. \"Parecen inviernos, ya casi ni las cobijas de lana de borrego nos abrigan en las noches\".El lugar donde nació ya no es el mismo de cuando era joven o niño. Con María Guamán, su hermana, recorría los caminos y al paso se encontraba sapos que se atravesaban en el trayecto, eran tantos que unos estaban sobre otros. Los habíaPRÁCTICA ACI: Aplicación de materia orgánica Materia orgánica para alimentar a la tierra de colores variados, verdes con blanco, tomates, negros, grises. El que más recuerdan es el de barriga verde.La variedad de sapos ha decaído. María Guamán, a sus 68 años, dice que ahora ya no los encuentra, que solo quedan en los pozos o en los bosques. A julio y agosto los conocían como los meses del viento y los esperaban preparados. Hoy esos meses sorprenden con cualquier fenómeno climático, a veces muy extremo.Por el lugar se siembran papas, ocas, habas, melloco, mashua, cebada y últimamente maíz. La gente de los alrededores pertenece a la Asociación Asopropangor y cuando se enteraron que el proyecto \"Papa, Familia y Clima\" promovía la Agricultura Climáticamente Inteligente (ACI) buscaron ser parte de él. Averiguaron, llamaron, buscaron y encontraron. Conocen de primera mano todas las prácticas ACI que les permiten mejorar la resiliencia y productividad de la agricultura familiar.La nostalgia por el clima que les permitía programar sus siembras y cosechas, el deseo de ahorrar en insumos y mejorar su economía familiar les inspira a practicar las enseñanzas de los técnicos del INIAP y del CIP. Por eso, para empezar su nuevo cultivo utilizando esquemas de fertilización inteligente, recogieron bastante estiércol de sus animales.José y Doralitza llevan esta materia orgánica a su terreno para enriquecer la tierra y empezar la siembra. Lo hacen seguros de obtener mejores resultados en sus cultivos, menores costos de producción y mayores ingresos para sus familias, al tiempo de conservar y mejorar el recurso más valioso para la producción de alimentos: el suelo. Doralitza Fernández coloca estiércol de animales en el suelo, para favorecer el crecimiento de la papa.Este plato tradicional de la parroquia se prepara con gallina de campo y es el banquete ideal cuando hay fiestas o llegan visitas especiales. Se acostumbra prepararlo también cuando los pobladores hacen mingas para sembrar papas u otro producto. Se lo considera un plato fuerte y suculento que puede preparse de manera individual o con la ayuda de la gente de la comunidad. Para dar vistosidad y sabor al plato, se lo acompaña con un picadillo de cebolla blanca, cilantro o perejil. Las papas que sorprendieron a Baltazara PRÁCTICA ACI: Labranza de conservación Gracias a su participación en el proyecto \"Papa, Familia y Clima, en la cosecha pasada, 12 familias de su comunidad lograron producir papas usando abonos orgánicos y otras técnicas de Agricultura Climáticamente Inteligente (ACI). Sembraron 10 quintales de semilla y obtuvieron 60 quintales de tubérculos. Ahora, van por la segunda siembra María Baltazara sostiene un canasto con papa chaucha amarilla, el producto de cultivo más común en Ajospamba.implementando y mejorando las prácticas ACI y ahorrando parte del dinero que gastaban en la compra de agroquímicos.María Baltazara Llongo recuerda lo que le enseñaron sus abuelos, a cultivar de manera natural. Así la papa es \"más rica y dura más\"; adicionalmente, no contamina el agua y los suelos.Sus amigos de la comunidad conversan con ella y le cuentan sobre la recuperación de esta forma ancestral de sembrar. Ella los mira, sonríe levemente con un gesto de satisfacción, mientras dice que \"sin papa no hay comida\".Esta comunidad cultiva en las laderas de las montañas de los Andes. Nelson Caizaghuano Shagñay está listo para la segunda siembra, utilizando el esquema conocido como labranza de conservación. Este tipo de agricultura consiste en mover lo menos posible los suelos para que no pierdan sus propiedades ni se erosionen, incorpora los residuos de la cosecha anterior u otros abonos verdes para aumentar su contenido de nutrientes y capacidad de absorber agua, y maneja la rotación de cultivos para aumentar la agrobiodiversidad: dejar \"descansar al suelo\" del mismo cultivo y minimizar la incidencia de plagas y enfermedades.Convencido de los beneficios de la agricultura de conservación, Nelson toma su azadón y empieza a preparar delicadamente el suelo, que se ve de un color negro intenso.Los platos elaborados con papa chaucha amarilla, o más conocida como limeña, son parte del consumo diario de la comunidad. Usan este producto para todo tipo de preparados como sopas, papas hervidas, fritas, tortillas y la conocida papa dormida, que consiste en cortar las papas y freírlas.Es usual que acompañen los platos con una bebida caliente dulce, que bien puede ser un morocho, arroz de cebada o colada de machica. Cocine la papa en leña por cinco minutos. Si lo hace en una cocina a gas, recuerde que esta papa es de rápida cocción y debe poner atención para que no se pase. 2. Corte la cebolla, el pimiento verde en pedazos largos y el cilantro. Sofría junto con la manteca de chancho por dos minutos. 3. Agregue la papa cocinada y revuelva para que se mezcle de manera uniforme. Añada sal al gusto. 4. Prepare la fritada de acuerdo a su costumbre. Una receta tradicional es cocinar la carne de cerdo en agua hirviendo con condimentos varios como ajo y comino. Luego fría con la grasa del cerdo en una paila o sartén 5. Al momento de servir, puede acompañar con quesillo y arroz de cebada de dulce con leche. ","tokenCount":"7679"} \ No newline at end of file diff --git a/data/part_6/0089031415.json b/data/part_6/0089031415.json new file mode 100644 index 0000000000000000000000000000000000000000..4bc9c1675b3bf691a7a45f9666a482be52705df8 --- /dev/null +++ b/data/part_6/0089031415.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"32614ffa39e3ec1e0532c9d99af55e8b","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/f65ba52b-9c07-4ec3-bc34-14043bb4602a/retrieve","id":"-1094884193"},"keywords":[],"sieverID":"bd50c16e-a643-4709-bfbf-edbdadf9f054","pagecount":"13","content":"Over the last decades, sheep population in Tunisia has been increasing reaching over 6.5 million heads in 2016 (Ministry of agriculture, 2016) with the Barbarine sheep as the dominant breed. This fat tail breed has an adaptive capacity that allows animals to cope under harsh environmental and sub-optimal husbandry conditions (Ben Salem et al. 2011).In Tunisia, sheep population face many health challenges including parasitic infections such as toxoplasmosis (Gharbi et al. 2013), fasciolosis (Akkari et al., 2011), lungworms and gastrointestinal helminths (Akkari et al., 2012). Gastrointestinal nematode (GIN) infections affect the welfare and productivity of small ruminants and are responsible for huge economic losses (Waller, 1997a). These losses are consequent to decreases in weight, reduction in milk yield as well as wool and mortalities can occur (Soulsby, 1983). GIN infections are increased in regions where extensive grazing is practiced (Waller, 1997b). Haemonchus spp. is one of the major and the most prevalent abomasal nematodes of small ruminants (O'Connor et al., 2006) and its prevalence increases in countries of temperate regions (Van Dijk et al., 2008). Akkari et al. (2012) showed that, in North Tunisia, 45.5% of the parasite population were found in the abomasum, with the prevalence of Haemonchus contortus exceeding 35%. This parasite represents a major problem in most flocks as it has developed resistance against most available anthelmintic drugs.The existence of genetic variation amongst sheep and goats in resistance to GINs has been studied by many authors and it has been demonstrated in several situations that genetic improvement could offer a solution to control haemonchosis. Bishop (2011) describesresistance to infection as the host's ability to interact with parasite and control its lifecycle. In the case of nematode infections, probabilities of ingested larvae, parasite development within the host, parasite mortality, parasite fecundity and faecal egg count (FEC) are included in resistance.The study of nematode resistance is first based on phenotypic measurements. The main indicator used for resistance to GINs is FEC. Nematode resistance assessed by using FEC has a low to high heritability in small ruminants, ranging from 0.01 to 0.65 (Zvinorova et al., 2016).In addition to FEC, several indicator traits could be considered in resistance to nematodes. In fact, measurement of anemia can be used as indicator for resistance in animals infected with H. contortus. Anemia can be measured using packed cell volume (PCV) or Famacha score which are heritable in sheep (Baker et al., 2003;Mandonnet et al., 2006;Riley and Van Wyk, 2009).Quantitative trait loci (QTL) mapping can also be used to understand the resistance to parasites. This technique allows identification of candidate genomic regions. Microsatellite markers (Marshall et al., 2009), microarray and genome-wide association studies (Brown et al., 2013) were used in small ruminant breeds to identify genes implicated in the control of resistance. Some of the genes commonly implicated in immune response such as interferon gamma (Dervishi et al., 2011) and major histocompatibility complex loci (Hassan et al., 2011) were also shown to be involved in the genetic resistance to GIN. In addition, several QTL on different regions and chromosomes (OARs) have been reported by many authors (OAR1, 3, 6, 14, 20) (Beh et al., 2002;Dominik, 2005;Crawford et al., 2006;Davies et al., 2006;Matika et al., 2011;Salle et al., 2012). In few studies, some potential candidate genes were identified on OAR8 (Crawford et al., 2006), OAR13 (Beraldi et al., 2007), and OAR22 (Silva et al., 2012).In Africa, the resistance of the Red Massai breed has been demonstrated (Baker and Gray, 2004). In Tunisia, where GIN represent a huge problem, the genetic resistance to haemonchus contortus has never been studied. The aim of this work is to study the genetic resistance of the Barbarine sheep to Hemonchus contortus infestation. Phenotypic measurements (age, breed, origin, anthelmintic use, diarrhea, anemia, management type, hematological parameters, biochemical parameters, FEC and abundance of infestation) will be performed and will be compiled in a single phenotype database. Genome analysis will be carried out using the 600K SNP Chip which will provide a better resolution of the sheep genomic profiles.This study will be carried out in four districts (Tunis, Bizerte, Beja and Jendouba) in the North of Tunisia (Figure 1). The choice of the geographic area was based on a previous study (Akkari et al. 2012) targeting regions where gastrointestinal parasites, and particularly nematode infestation, represent one of the main constraints in small ruminants' production. Previous studies have shown that smallholder sheep farmers do not deworm their animals using anthelmintic-based products and because exposure is very high and sheep still manage to survive and produce in these environments. Therefore, the likelihood of finding individuals with some kind of resistance is relatively high.During August, September and October 2017, abomasa will be collected from 300 to 400 sheep, aged more than 6 months, from slaughterhouses located in the North of the country (Figure 1).Before slaughter, animals will be identified and blood samples collected in EDTA, heparin and dry tubes via jugular venipuncture. Fecal samples will also be collected from each animal.Information concerning age, sex and breed. The presence of diarrhea, anemia or other symptoms will be noted in the data sheet (Annex 1).Abomasa will be ligated at both ends then transported to the Laboratory of Parasitology at the National Veterinary School of Sidi Thabet (Tunisia) in cooler boxes. Larvae (L3) (both Haemonchus and other parasite species: Ostertargia and Trichostrongylus) will be collected from the abomasum. Parasites will be preserved in 70% ethanol until examination.The identification of gastrointestinal parasites will be made using a microscope equipped with eyepiece micrometer. Prevalence, intensity and abundance of infestation will be estimated as follows:Prevalence of infestation = 100 x number of infested sheep/number of examined sheep Infestation intensity = number of collected larvae/number of infested sheep Abundance of infestation = number of collected larvae/number of examined sheepThe following hematological parameters will be estimated for each animal: White blood cells (10 9 l -1 ), hematocrit (PCV) (%), red blood cell count (×10 12 m -1 ), hemoglobin (g d -1 ), Mean Corpuscular Volume (MCV) (fl), Mean Corpuscular Hemoglobin (MCH) (pg), Mean Corpuscular Hemoglobin Concentration (MCHC) (g dl -1 ), Red Blood Cell Distribution Width (RDW), Index of Red Blood Cells Distribution (IDR) (%), Platelets (10 9 l -1 ), Average Platelet Volume (VPM) (fl), Index of Platelets Distribution (IDP) and Plaquettocrite (Pct) (%) will be estimated using an Auto Hematology analyser BC-2800Vet® (ShenzenMindray Bio-Medical Electronics Co., Ltd, Hamburg, Germany).The presence and type of anemia will be recorded (Blood & Radostitis, 1989).Plasma will be recovered from the heparin tubes and used to estimate biochemical indicators: albumin (g/L) and total proteins (g/L) with a Random Access Clinical Autolyzer® (Dialab, Vienna, Austria).Fecal samples will be processed by flotation (McMaster Egg Counting Technique). Fecal Egg Count (FEC) will be performed on 5 g of feces using saturated solution and each egg counted representing 50 eggs. In order to identify eggs, coprocultures from each animal will be prepared and incubated at 22-25 •C for 7-10 days to provide third stage larvae (L3) which will be harvested by the Baermann technique. The composition of each coproculture will be examined by microscopically. The identification of fasciola and other gastro-intestinal parasites will be performed.Animal's DNA extraction DNA will be extracted from 300 µL of the blood of each sheep using the Wizard ® Genomic DNA purification kit (Promega, Madison, USA) according to the manufacturer's instructions and stored at -20°C for genome analysis.DNA will be extracted from the collected H. contortus and other worms. After washing in PBS, worms will be ground with a pestle in liquid nitrogen in 1.5 ml microcentrifuge tube. Proteinase K will be added and the mix will be incubated overnight at 56°C. DNA will then be extracted using Wizard ® Genomic DNA purification kit (Promega, Madison, USA) according to the manufacturer's instructions then stored at -20°C until used.In order to verify the presence of piroplasms (Babesia and Theileria) and if anemia is only caused by gastrointestinal parasitism, Giemsa-stained blood smears will be examined under a microscope with immersion oil at 1000 magnification. For each slide, 50 microscopic fields will be examined.In addition, Catch-all primers (RLB-F and RLB-R) which detect Theileria spp. Babesia spp.and Anaplasma/Ehrlichia spp. pathogens will be used. Reactions were performed in 25 µl volume containing 1 x PCR buffer, 1.5 mM MgCl2, 200 µM of each deoxyribonucleotide triphosphate, 0.125 µg of Taq hot start Ab, 0.1 U of Uracil DNA glycosylase, 25 pmol of each primer and 1.25 U of Super Taq DNA polymerase (Vivantis, Chino, CA, USA). Forty PCR cycles will be performed with a thermocycler (ESCO Swift MaxPro). Each cycle consist of a denaturing step of 1 min at 94°C, an annealing step of 1 min at 50°C, and an extension step of 1.5 min at 72°C. A final extension step of 10 min at 72°C will complete the program.This molecular technique will detect the presence of tick-borne pathogens in sampled animals.Blood, Feces and abomasa were collected from 304 animals belonging to mainly four breeds: Barbarine breed, the main sheep breed in the country which is at-tailed breed characterized by metabolic and digestive adaptations to the contrasting environmental conditions in Tunisia (Ben Salem et al. 2011), Queue fine de l'Ouest breed, Cross bred individuals between the two previous breeds and finally Noire de Thibar, mostly present in the North of the country. Age, sex and breed were recorded for each animal.All abomasa were opened along the greater curvature and the contents were washed into a bucket and then they were carefully examined for the presence of Haemonchus and other gastro intestinal worms. The counting of the parasites of each abomasum was realized, males were separated from females and will be used for morphological identification. All worms from each animal were preserved in 70% ethanol in order to be used for DNA extraction.The number of parasites in each abomasum of each animal was recorded in the database.Haemonchus parasites are already identified and the number of this species in each abomasum was recorded. For other gastrointestinal nematodes males are used for identification and the work is in progress.All hematological parameters (haematocrit (PCV), red blood cell count, haemoglobin, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, Red Blood Cell Distribution Width, Index of Red Blood Cells Distribution, Platelets, Average Platelet Volume, Index of Platelets Distribution and Plaquettocrite (Pct) were estimated for each animal using an Auto Haematology analyser BC-2800Vet® (ShenzenMindray Bio-Medical Electronics Co., Ltd, Hamburg, Germany) and were recorded in the database.Albumin (g/L) and total proteins (g/L) were estimated with a Random Access Clinical Autolyzer® (Dialab, Vienna, Austria) and recorded in the database.The coproscopic survey was realized for each feces sample and qualitative coprology allowed identification of gastro intestinal eggs and other eggs which are Trichures, Cocidies, Nematodirus, Moneizia and pulmonary larvae. Quantitative corology examination allowed the counting of these eggs and this information is uploaded in the database.After dissection of the abomasa, 240 samples were collected from normal and affected tissues. Tissue samples were fixed in 10% formalin and processed for a routine histological examination. Samples will be stained with hematoxylin Eosine and examined under microscope for the presence of specific lesions. A correlation will be established between the infestation pattern and the microscopic lesions.Animal's DNA extraction DNA from the blood of each animal was extracted using the Wizard ® Genomic DNA purification kit (Promega, Madison, USA) according to the manufacturer's instructions and the concentration was measured using a Spectrophotometer. DNA concentration was recorded in the database. DNA was stored at -20°C for genome analysis.Parasite's DNA extraction will be done at the end of the step of the morphological identification.Catch-all PCR using RLB-F and RLB-R primers which detect Theileria spp. Babesia spp are yet to be carried out.The database on phenotyping indigenous sheep breeds for gastro-intestinal parasites concerned 304 sheep from 4 different breeds and several locations, all in the North of Tunisia. Compilation of the database has now well progressed and should be finalized soon. In summary, phenotyping includes information related to each individual animal which are age, breed, origin, anthelmintic use, slaughter reason, feeding scheme, diarrhea, anemia, management type (grazing, grazing and supplementation, intensive), hematological parameters, biochemical parameters, fecal egg count, abundance of infestation and existence of parasite-induced lesions in the abomasa. DNA, for genome analysis using the 600K SNP Chip, was extracted from the blood of each sheep and first data on genotyping should be made available in 2018. ","tokenCount":"2051"} \ No newline at end of file diff --git a/data/part_6/0090347379.json b/data/part_6/0090347379.json new file mode 100644 index 0000000000000000000000000000000000000000..f95862f7ff79d37487ebc654f20b0e60db86be81 --- /dev/null +++ b/data/part_6/0090347379.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"d609e8298b2e3ecd1652ceb9572ef439","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/77413884-d12d-4e4e-a84b-73abc7479949/retrieve","id":"1060874602"},"keywords":[],"sieverID":"022c42ad-efc7-42e7-82a7-d83e2caf1b5c","pagecount":"26","content":"• Carbon Dioxide (CO2): CO2 is the most prevalent greenhouse gas emitted by human activities.-It is primarily released through the burning of fossil fuels such as coal, oil, and natural gas for energy production, transportation, and industrial processes. • Methane (CH4): Methane is a potent greenhouse gas with a higher warming potential than CO2 over a shorter time frame.-It is emitted from various sources, including livestock digestion, agricultural practices, natural gas production and distribution, and the decay of organic waste in landfills. • Nitrous Oxide (N2O): N2O is released from agricultural and industrial activities, as well as the burning of fossil fuels and solid waste. • The IPCC assesses GHG emission, atmospheric CO2 conc., develops emissions scenarios to explore different trajectories of future GHG emissions and their potential impacts on climate change.-Help policymakers and researchers understand the range of possible futures based on different socioeconomic and technological pathways.• Energy Sector: 35% to 75% of global CO2-equivalent emissions, includes Electricity and Heat Production, fossil Fuel Combustion (coal mining, oil and gas extraction)• Industry: Contributes to around 20% to 30% of global emissions. This sector encompasses emissions from manufacturing processes, cement production, and chemical industries.• Transportation: Road Vehicles: Contribute to around 14 to 24 % of global CO2-equivalent emissions.• Livestock Sector: Contributes to roughly 14% to 18% of global emissions. This includes emissions from enteric fermentation (digestive processes) in ruminant animals (such as cows and sheep), manure management, and other livestock-related activities.• Agriculture (Non-Livestock): Contributes to about 7% to 14% of global emissions. This sector includes emissions from rice cultivation, synthetic fertilizer use, crop residue burning, and other agricultural practices excluding livestock• Residential and Commercial Buildings: Contributes to approximately 6% to 17% of global emissions. Emissions arise from energy use for heating, cooling, lighting, and appliances in residential and commercial buildings.• Waste Management: Contributes to around 2% to 5% of global emissions. Emissions mainly come from the decomposition of organic waste in landfills and the release of methane gas.• Productivity:-Improved livestock breeds that are more productive and resilient to climate stresses -Implementing sustainable intensification practices • Adaptation:-Climate change is already impacting agricultural systems worldwide, and adaptation measures are necessary to build resilience and minimize the negative impacts -Developing climate-resilient livestock breeds that are better adapted to changing climatic conditions. -Enhancing early warning systems and climate information services to support farmers • Mitigation:-Improving livestock breeding/management practices to reduce enteric fermentation and manure-related methane emissions -Genetic selection for lower emission, feed efficiency and lower methane production per feed intake -Improved feeding practices to reduce methane emission ration of maternal permanent environmental effects to the total phenotypic variance; h 2 t total heritability; r am genetic correlation between direct and maternal additive heritability; σ am .covariance between direct and maternal additive genetic effect; CV A additive coefficient of variance; Log (L) log Likelihood. BW: birth weight, WW: weaning weight, 6MW: six months weight, YWT: yearling weight ADG1: average daily gain from birth to weaning, ADG2: average daily gain from weaning to six months, ADG3: daily gain from six months to yearling. ","tokenCount":"506"} \ No newline at end of file diff --git a/data/part_6/0091453669.json b/data/part_6/0091453669.json new file mode 100644 index 0000000000000000000000000000000000000000..d698939d2a7f49d60066a41e0865ea4fe8566c42 --- /dev/null +++ b/data/part_6/0091453669.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"a164e456313cc66ae3bc5533c21f6bcd","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/0b493ec0-642e-4752-91aa-ed67640e5b34/retrieve","id":"311764753"},"keywords":[],"sieverID":"c0ecec02-446c-49c2-a1ad-1fb9af28178d","pagecount":"2","content":"The hubs are demand driven units and provide intelligence and technical backstopping to farming communities, national and international research programs, development actors, seed companies and policy decision-makers, on resilient seed systems tools, methods, information sources, data management, policy design and compliance with applicable regulations and agreements. Functions include: facilitating establishment of new links among interested seed system actors; support research and training; develop and maintain a digital seed knowledge portal; develop harmonized principles/agreements and policies.• Guidance on enhancing institutional capacities and enabling conditions for scaling food and nutrition security under climate change is disseminated and policy dialogues held in selected countries","tokenCount":"101"} \ No newline at end of file diff --git a/data/part_6/0100820480.json b/data/part_6/0100820480.json new file mode 100644 index 0000000000000000000000000000000000000000..9344433cb4199a52ed4a4a08410dd3fb7d533318 --- /dev/null +++ b/data/part_6/0100820480.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"bbb3e1d6833ad0a202d337e74a1a228e","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/336d402e-a6aa-4911-8dee-cec8be6923db/retrieve","id":"-59294014"},"keywords":["Livestock production","silvopastoral systems","soil macrofauna","soil quality indicators"],"sieverID":"5e2943bf-6009-4fa1-bebf-e1ea3e3e4c4a","pagecount":"2","content":"Silvopastoral systems (SPS) are an environmentally and economically beneficial alternative to single grass systems for livestock production. The incorporation of trees, especially legumes, in pastures has been shown to have several positive effects on soil properties and nutrient cycling, while creating a favourable microclimate for the animals and increasing the productivity (i.e. milk and meat). The inclusion of legumes or legume-trees in the pastures systems leads to improved nutrient cycling and increased biological activity creating fertility islands within the SPS. Soil macrofauna has been considered as bioindicator of soil quality because of the direct effect on soil properties and on soil organic matter fragmentation and nutrient dynamics. The present study was performed in an experimental block designed (n=3) (silvo)pastoral trial located at CIAT (Colombia) and aimed to evaluate the effect of the inclusion of the herbaceous legume (Canavalia brasiliensis) and both herbaceous and shrub legume (Leucaena leucocephala) respect to the Brachiaria cv. Cayman monoculture on a set of biological and physical parameters. We measured the abundance and diversity of soil macrofauna, macroaggregate morphology and soil aggregation, as well as their spatial heterogeneity in relation to the trees in the SPS. Soil samples were collected at three different distances from the Leucaena doble-row as follows: i) between the rows, ii) at 1.5 m and iii) 5.5 m from the trees. Results obtained showed that the inclusion of legumes has a positive effect on soil macrofauna with the highest abundance found at 1.5 m distance from Leucaena. On the contrary, reduction of total abundance was found around the trees corresponding to higher soil compaction areas probably due to the animal grazing preference and search for shade. This phenomenon was reflected in higher proportion of physicogenic aggregates and lower amount of large water-stable macroaggregates. In addition to increased productivity of legume-based pastures and the potential to sustain higher densities of animals, our results highlight the importance of an integrated evaluation of spatial heterogeneity within SPS and discuss possible consequences for the management of trade-offs. Multivariate statistics of forthcoming data will reveal possible role of soil macrofauna as a reliable soil quality indicator.","tokenCount":"349"} \ No newline at end of file diff --git a/data/part_6/0101920677.json b/data/part_6/0101920677.json new file mode 100644 index 0000000000000000000000000000000000000000..004aefcb8487c522bd8cdaf96618a70f2b8f340f --- /dev/null +++ b/data/part_6/0101920677.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"6764f64e55c4d13d8fa4595d39dbf301","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/410a8b8a-01fd-4845-9f37-5456072e2ce4/retrieve","id":"1376636143"},"keywords":[],"sieverID":"2a759616-8acc-498f-9d55-73ef786377e2","pagecount":"4","content":"CEA) is the production of plants, fish, insects or animals inside structures, such as greenhouses and buildings, in controlled conditions. In a rapidly urbanizing world, CEA can contribute to sustainable development, e.g. through reduced use of land, water and inputs. There is a need for innovation in policy, technology and business practices to scale up CEA in the Global South sustainably and equitably.n Economic agencies should invest in development of supply chains to support CEA, including growing media, equipment and seeds, and postharvest infrastructure such as cold storage, throughfor example -business support and mentoring, business incubators and tax breaks.n Regional and national governments should form public-private partnerships (PPPs) for the development of regional CEA clusters or tech hubs, enabling growers to share experiences, innovations and information, leverage economies of scale, and market collectively.n National and local governments should acknowledge CEA as a viable form of agriculture and design policy innovations to promote the sector, including in agriculture development policy; land use and planning policy; economic development and employment plans; and import regulations.n Technology developers should dedicate R&D spend to trialing their inventions with growers in low and lower-middle income countries, to ensure they are optimized for these contexts and to provide access to new, environmentally safe, developments as early as possible.The challenge: CEA needs to be a force for sustainable and equitable development For CEA to be a viable option for people from less affluent backgrounds, financial institutions including banks, micro-finance institutions and parastatal agricultural finance agencies should invest in people as well as equipment by designing innovative debt financing models for entry-level, small-scale CEA practitioners. These may include:n Provision of equipment to set up operations, as well as provision of welfare and living costs over an initial period, so that new starters can cover everyday expenses n A payback period that is customized to CEA growing cycles with repayments beginning after the activity starts to be profitable n In cases of contract farming, three-party agreements between lenders, borrowers and buyers, with the latter guaranteeing a market for the borrowers' produce.Grant-making bodies, NGOs and commercial financial institutions that work in Africa and Asia should promote research and innovation through dedicated CEA agribusiness/agripreneur programs and incubators under their agricultural development programs. These may include preferential grant or loan schemes that are tailored to the needs of women, young people and applicants from disadvantaged social groups.Opening CEA to people from a range of backgrounds and socio-economic groups will promote poverty reduction and provision of viable livelihoods for people who currently lack economic opportunities.In addition, locally appropriate CEA techniques should be included in educational programs at all levels, from elementary school to agricultural universities. The installation of demonstration gardens could provide produce for the local community, as well as enable students to develop valuable STEM (science, technology, engineering and math) skills, and increase the pool of potential employees for CEA businesses as they scale up, expand or replicate in new locations.The self-organization of CEA practitioners into associations or cooperatives (local, regional or national), if necessary with help from development organizations and NGOs, can enable peer-to-peer support, facilitate valuechain development (ensuring availability of inputs and equipment), and allow practitioners to collectively identify their needs and lobby their governments to address them.It can also optimize their access to investors who are unable to deal with individuals.Organization may also be formal, through PPPs for the development of regional CEA clusters or tech hubs where growers can work collectively or in close proximity, sharing experiences and information (e.g. on optimal technologies or disease management), leveraging economies of scale on equipment and inputs, and marketing collectively.Clusters require significant investment in infrastructure (structures, water, etc.), innovative mechanisms to make public or private land available, and incentives for growers to move to the area (tax reduction for initial periods, business support, etc.).Another formal support mechanism is the provision of CEA training by agriculture departments, tailored to specific local needs, regularly updated to include emerging technologies so that the latest knowledge reaches people in low and lower-middle income countries.Agricultural extension services should ensure agents are knowledgeable in CEA techniques so they can identify problems post-setup and know how to help. New, innovative extension models may also be developed to facilitate knowledge exchange between early adopters and extension officers, as well as formalize direct peer-topeer exchange between early adopters and new starters.Collectivity and dedicated support mechanisms will benefit individual CEA practitioners by helping them to overcome operational hurdles and reducing the risk of failure. These mechanisms will stimulate development of the sector as a whole, from vertical farms in slums to hightech container or rooftop farming.At the local level, zoning ordinances and urban agriculture regulations should include specifications on CEA so that there is clarity on what is permitted and where. CEA may also be integrated into spatial design and building codes.At the national and regional levels, governments can create an enabling environment for CEA adoption and mainstreaming through policy innovations in several areas. For example:n Agricultural policy can advance mainstreaming of CEA, through funding provision and extension capacity n Food security and nutrition strategies can recognize the contribution of CEA, especially for ensuring local supply that is less vulnerable to disruptions and promoting year-round stable prices n Employment strategies may recognize and promote employment opportunities in CEA, including the need to develop suitable skillsets for all supply chain roles n Land use policy can acknowledge CEA as a legitimate activity, removing any barriers to land access accordingly.In addition, national governments should develop evidence-based industry standards and regulations, through cooperation between relevant government departments, the private sector and NGOs to ensure they are conducive, relevant and appropriate. These will enable farmers to plan their activities and support a good reputation for the sector. Early development of standards and regulations will pre-emptively discourage harmful or fraudulent practices and help to avoid excessive or punitive regulations in the future.Regulatory standards on the nutrients required in hydroponic growing should be used as a reference for customs inspections to avoid unwarranted import bans or tariff inconsistency. The removal of several regulatory barriers to CEA in a concerted, integrated way will create an enabling environment for practitioners to operate close to urban markets and access inputs, training, extension support and human resources.There is a need for ongoing research into CEA techniques to minimize energy consumption and costs, and reduce use of synthetic or environmentally unfriendly inputs, while optimizing efficiency. As optimal techniques will vary depending on local context, such research should be carried out by local and/or international universities and agricultural research centers in partnership with local CEA growers, and funded by public institutions.The inclusion of CEA in the official overseas trade and development programs of (high income) countries with strong CEA sectors is an innovation that would encourage private CEA companies and technology developers to invest in new (low and lower-middle income) markets, where their solutions can be adapted and adopted to suit the local contexts. This may include dedication of R&D spend to trials of new inventions by African and Asian practitioners to ensure they meet their needs and environmental regulations, and to provide access to new developments as early as possible (especially equipment to monitor or survey crops, and equipment for post-harvest processing and cold storage to reduce food waste and environmental footprint).Where equipment costs cannot be reduced to be immediately affordable by small-scale producers in Africa and Asia, technology companies could help by devising hire-purchase schemes that would enable operators of limited means to access equipment immediately.CEA is not a silver bullet for food security or agrifood system sustainability or equity. It is unlikely to replace open field agriculture, nor render urban areas self-sufficient in fresh produce, but as a form of urban farming it has potential to complement rural systems' ability to deliver fresh produce and niche commodities, for both low-end and high-end customers. With increased awareness, innovative forms of targeted investment, and supportive policies, the application of optimal, appropriate CEA techniques in each context can transform livelihoods and environmental outcomes and contribute to urban diets.Huge technological advances on how to grow food close to consumers, where land is in short supply and conditions are inhospitable, must be made available to communities that stand most to benefit from them.A small but growing number of entrepreneurs are taking up CEA in urban and peri-urban areas across Africa and Asia. These pioneers often learn techniques by watching YouTube videos and apply them using a trial-and-error approach. They are generous with their knowledge, running free or affordable training courses and building their own communities of practitioners from the ground up. These pioneers, their protégés, and the sector as a whole would benefit from the concerted efforts of multiple actors to remove entry barriers and ensure operational viability of CEA, and to promote CEA cultivation of local crops that are accessible and affordable to all. For more information, see the full report at: https://hdl.handle.net/10568/117234Supported by:","tokenCount":"1479"} \ No newline at end of file diff --git a/data/part_6/0108471149.json b/data/part_6/0108471149.json new file mode 100644 index 0000000000000000000000000000000000000000..73c6d7edbfd63c9e2f93f4724e5bc7e86ba47ebf --- /dev/null +++ b/data/part_6/0108471149.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"a3776bae3f7368d7c37f0e59a6352197","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/a2d454e7-ce96-47dd-8ed3-26b2238d0a91/retrieve","id":"2104252635"},"keywords":[],"sieverID":"6eec970b-e242-4bbc-9f67-40d1dc811f80","pagecount":"14","content":"Titles in this series aim to disseminate interim climate change, agriculture, and food security research and practices and stimulate feedback from the scientific community.Egerton University, a member university of the Regional Universities Forum for Capacity Building in Agriculture (RUFORUM), held an engagement meeting with Technical Vocational Education and Training (TVET) institutions on 6 th May 2022. The meeting was held under the auspices of the University-TVET forum. This forum sought to enhance the collaboration and working partnership between universities and TVET institutions to strengthen the education value chain. The University-TVET forum is part of an arrangement championed under the RUFORUM's Transforming African Agricultural Universities to meaningfully contribute to Africa's growth and development (TAGDev) programme.The Forum was convened with four objectives;To identify areas of comparative advantage for each ATVET institutionTo identify areas of complementation between Egerton University and TVET institutions •To strategize the modalities for capacity building and co-creation o • f curricula in climate-smart agriculture and entrepreneurship.To explore the participation of universities in the curriculum review and accreditation process to be undertaken by TVET institutions.The Forum was introduced to the Accelerating Impacts of CGIAR Climate Research for Africa (AICCRA) project (https://aiccra.cgiar.org/), a CGIAR initiative currently coordinated by the Alliance of Bioversity International and CIAT (https://alliancebioversityciat.org/). The AICCRA project seeks to build up the technical, institutional, and human capacity of targeted regional countries to strengthen resilience to climate change impacts by increasing the dissemination and uptake of CGIAR and partners' research. Through this initiative, the CGIAR will focus its interventions in Kenya, Ethiopia, Zambia, Senegal, Ghana, and Mali with a regional benefit through the RUFORUM for higher education institutions.RUFORUM is one of the implementing partners to the AICCRA project with a focus on higher education institutions for implementing climate-smart agriculture, including; designing and reviewing courses to integrate climate-smart agriculture and information services. RUFORUM, in collaboration with Egerton University, has strategically focused on engaging the ATVETs because of their central role in training the middle cadre agricultural workers that are central to the delivery of agricultural extension services. Secondly, ATVETs are critical players in influencing the adoption of various agricultural technologies, innovations, and management practices among farming communities.The four institutions that responded were Baraka Agricultural College started in 1974 as a farmers training place. It responded to the lack of skills as the white farmers were going back. The St. Franciscan Brothers were given complete authority. The Franciscan brothers were originally from Ireland, Piggery.The purpose of the training was to sell seeds to different farmers; this enabled the institution to be known by other farmers. During the recruitment, it provided the opportunity to get farmers from Baringo and Nakuru. Baringo is a bit dry, but some areas receive rain. They took the approach of engaging other agencies to help with recruitment. They focused on communities/persons that are marginalized. Identified the activities that the youth were involved in and looked out for the people with the motive.Six weeks of training worked on the expectations of the farmers. This enabled the focus of the first two weeks on the foundations of sustainable agriculture. The skills included: Understanding the resources and their sources, understanding the changing circumstances/conditions, handling the soils, including identifying good soils through soil sampling and management of soils. Another skill was terracing because of the hilly locations. Through this, they are able to help the communities.Knowledge of crops, with a focus on requirements for vegetable production; they were taught how to make compost for cropping so that they could begin to appreciate and use it. Other skills imparted were fruit identification against climatic conditions, seeds identification, sowing, potting, nursery management, managing fruits and diseases, harvesting, and post-harvest handling, especially processing farm produce in plenty. Milk and the production of yogurt are aimed at job creation. Fruit juice making to cater to the plenty of fruit harvest. Beekeeping skills include; siting of the apiary and the type of bee hives used.Selected enterprises based on time are dairy, poultry, and beekeeping. Further, it extended to feed formulation that applies to dairy, poultry and fish farming. Skills gained include Slaughtering. Feed conversation; silage making and haymaking, developing a formula, their own formula, and feeding regimes. Selection of different livestock enterprises based on market demand. Further, how to identify each animal based on need. Disease control and basics on treatment. Constructing houses for different livestock. Establishing different pastures and management. They were trained in the inspection of the meat. Land preparation for pastures, e.g., Brachiaria, etc. Breeding/reproduction skills were taught to get the trainees to appreciate basic issues such as; timely breeding records keeping, value addition, including how to produce cheese, value addition of carcass, fodder trees and intensification of fodder production. Heifer inspection, entrepreneurship, processing of honey and how to market. Entrepreneurship in terms of how to start their businesses, managing the business, cost-benefit advisory and then marketing.Income generating unit (generate income for the college's sustainability, units are resource units for trainees, brand the college through the products that the college produces). The farm is the main resource centre; upgrading of facilities used on the farm. Earlier, there was a curriculum developed through RUFORUM support focusing on piggery. For the curriculum to be offered, the facilities needed to be upgraded to a level acceptable to enable quality training; this has not yet been done. Similarly, the dairy unit needed an upgrade, e.g., silage slab, cow handling structure with slurry pit. Development of basic seed production facility because potato seed has a high demand that team is currently unable to meet the demand. The team further seeks to work on the tissue culture facility to multiply the basic seed. This will enable them to expand their production capacity from this year's 5 acres level. In addition, there was a need to enhance irrigation capacity within the college because the irrigation system was lacking. Accordingly, with support from the EU, the college has constructed a rainwater-harvesting pond, from which the water for irrigation will be obtained. The college has also moved towards operationalizing its green energy; this was deemed necessary by adopting solar for lighting and bio-digesters for biogas.The college undertakes an outreach program to contribute to increased food security, income, and environmental sustainability of rural communities. The college worked in three sub-counties. Activities focused on; water provision through the drilling and rehabilitation of boreholes and distributing water in a radius of 10 km and trained water community management committees. Capacity building in diversified food options among 650 households such that; 30% of these households have realized a reduction in household expenditure and 30% increase in household income. Further, the college has been involved in capacity building on nutrition and value addition. The outreach programme also trained smallholder farmers in entrepreneurship and savings. Capacity building on climate resilience within beekeeping as a flagship intervention around the Lakes and wildlife conservation areas (Lake Nakuru National Park), and the provision of energysaving devices such as the local cook stoves (jikos). Beyond agriculture and income, the outreach programme also involved public health education, sanitation and hygiene in parts of Kisii Ndogo, focusing on ending open free defecation. Other cross-cutting issues; in post-election leadership, conflict resolution, and peacebuilding.Avenues available for supporting Baraka college moving forward include; upgrading facilities, capacity building of staff from diploma to degree and degree to master, and capacity building in pedagogy to enhance teaching skills. Further support for curriculum development for levels 3, 4, and 6 and upgrading of science laboratory, including soil testing. They are establishing online learning systems since they are lacking and upgrading the online system and library.Baraka college has been engaged in CSA activities. The courses and activities that are CSA enabled include:• Organic farming; promoting practices such as controlled compositing, bio-fertilizers, biogas production • Tree planting; promoting action for carbon sequestration, and multipurpose trees for income enhancement • Apiculture-bee keeping; promoting environmental conversation/carbon sequestration, zero emissions production, and enhanced ecosystem services • Circular Organic waste management • Integrated pest management • Irrigation and water harvesting • Fish farming • Weather station for climate data collection • Value addition Some CSA-enabled courses include; Sustainable Agriculture for Rural Development (SARD), Apiculture, and modular training. Approach-short courses and outreach on apiculture, SARD, and Soil conservation. Baraka college further seeks to develop level 3, 4, and 6 courses.Baraka college team identified a number of gaps within its institution, including; limited digital competency for forecasting and early detection systems for weather/climate information and limited exploitation of CSA methods in animal production, e.g., vermiculture. Further, it was noted that CSA methods were not as effective as the conventional production approaches and were not competitive economically and practically. There were low adoption rates of CSAs because they are labor intensive and have low automation. There was a lack of premium pricing for products produced using CSA approaches and a limited innovation ecosystem around CSA.However, there were possible areas universities could work with TVETs/Baraka to address the gaps; they include;• Research and innovation • Capacity development in areas of weather and climate information • Funding for capacity development of staff and infrastructureThe Dairy Training Institute (DTI) has been engaged in CSA/I with several activities that included tree planting, circular economy through using fertilizers from animals, putting up solar panels, and engaging in value addition of produce.DTI has had several curricula that were CSA-enabled. These included:• Dairy production and processing (Diploma)• Dairy production and management (certificate) The meeting with ATVETs concluded with identifying a number of gaps that affected the pace at which CSA in their institutions is implemented as well as its adoption. These include:• Limited digital competency in forecasting and early detection systems for weather and climate information, lack of skills for accurately collecting, analyzing and interpreting climate data, and making use of an advanced source of climate information and services • Limited use of modern climate-smart agriculture practices such as vermiculture • Low adoption rates for CSA practices largely because they tend to be labor intensive and limited automation and have low returns on investment in large areas compared to the conventional approaches when deployed • A lack of a premium price on the market for products produced using CSA approachesThe ATVETs recommended that there were several areas of convergence for collaboration with universities in the field of climate-smart agriculture, including:• Capacity building of staff through the training of trainers in various aspects, including climate information services access, modern CSA practices, long and short-term specialized trainings for increasing the skills of staff, and skills in competitive proposals development for resource mobilization • Support for infrastructure improvement for advancing a circular economy. 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New Phytol. 91, 45-56 soil crusting, soil sealing, soil erosion, chicken manure, Inceptisols, tillage system decomposition, mineralization, quality factors, simulation, modelling soil organic matter, charge characteristics, multipurpose trees, CEC, decomposition fertilizer use, maize, missing nutrient trial, Olsen-P, on-farm level, particulate organic matter, pot experiment alley cropping, root abundance, root length density, root weight density, tap root profitability, manure storage, soil fertility, maize P use efficiency, rainfall, soil and crop management, Pearl millet, Cowpea, West Africa Soil physical characteristics, Oxisols, Infiltration, Organic matter, Rainfall acceptance, Lower and upper limits of available water agro-pastoral systems, crop rotation, soil degradation, soil improvement, soil physical vulnerability, tropical savanna Calliandra, fallows, Indigofera, slash and mulch, soil quality, Tithonia Oxisol, land-use system, sequential P fractionation, short term P dynamics, 33 P labeling, metallic (oxy)hydroxides, soil microbial biomass Acid soils, crop-pasture systems, crop rotations, soil P pools, vertical tillage soil fertility, legume cover crops, Mucuna, Canavalia, nutrient budgets farmyard manure, fertilizer, organic-mineral interactions, Organic Resource Database, percentage fertilizer equivalency values, plant materials, residual effects manure, decision guides, farmer perceptions soil organic matter, soil organic matter fractions, soil dispersion, and dispersing agent secondary forest","abandoned pasture","carbon sequestration","plant nutrient stocks","soil nutrient stocks","nutrient loss","Oxisol","succession","Amazon Amazon","Humid Tropics","Ferralsols","Leaching","Nutrient Cycling","Slash-and-Burn Africa, collective action, landscape, local knowledge systems, Latin America, participatory methodologies"],"sieverID":"ebb55e40-53d6-470f-9eb7-af3a06ad5f3d","pagecount":"654","content":"Pathways Towards Integration of Legumes into the Farming Systems of East African Highlands. T. Amede. (Draft Paper)………………………………………….. • Towards Addressing Land Degradation in Ethiopian Highlands: Opportunities and Challenges. T. Amede (Draft Paper)………………………………………….. • Phosphorus use efficiency as related to sources of P fertilizers, rainfall, soil and crop management in the West African Semi-Arid Tropics. Bationo A., and K. Anand Kumar. ………………………………………………………………………………… 6. Output 2: Improved soil management practices developed and disseminated. (Part 1, 1639 kb; Part 2, 494 kb) Papers • Use of deep-rooted tropical pastures to build-up an arable layer through improved soil properties of an Oxisol in the Eastern Plains (Llanos Orientales) of Colombia.• Yields in farmers fields increased.• Land degradation halted/reduced.• Yields per unit area and input increased.• Land use changed • Farmers surveys.• Regional/national production statistics.• Land use surveys (satellite imagery, rapid rural appraisal).• Land survey data available To develop and disseminate to clients strategic principles, concepts, methods and management options for protecting and improving the health and fertility of soils through manipulation of biological processes and the efficient use of soil, water and nutrient resources in tropical agroecosystems.• Technologies for soil improvement/ management developed. • Limiting soil-plant-water processes identified.• Compatible plant components identified for low fertility soils in crop-livestock systems. • Guidelines, manuals and training materials for integrated soil fertility management produced.• Scientific publications other projects (e.g., PE-3, BP-1) • Field sites accessible Output 1. Biophysical and socioeconomic constraints to integrated soil fertility management (ISFM) identified and knowledge on soil processes improved.• Soil, water, nutrient and knowledge constraints to sustainable production defined, and the understanding of the role of soil biota, multipurpose germplasm, and organic and inorganic resources for sustainable management of land resources improved.• Annual Report/ publications • Reviews published • Documents of synthesized results• Detailed tables published in Annual Report. • Decision guides for ISFM developed.• Sufficient operational funds for soil and plant analyses. • Literature on constraints available • Farmers continue to participate.• Projects SN-2, PE-3 and PE-4 actively participate. • Collaboration of participatory research project (SN-3), RII and NARS. Output 2. Improved soil management practices developed and disseminated:• Relevant knowledge, methods and decision tools for improved soil management to combat soil degradation, increase agricultural productivity and maintain soil health provided to land users in the tropics.• Annual reports/ publications.• Management guidelines and decision trees published and available to farmers, NARs, NGOs.• Training manual for use with tools.• Maps published.• Simulation models used to assess alternative management of organic resources for ISFM • A policy brief for ISFM produced.• Sufficient operational funds available for chemical analyses. Output 1. Biophysical and socioeconomic constraints to integrated soil fertility management (ISFM) identified and knowledge on soil processes improved• Relationships of organic input quality to fertilizer equivalency values established • Quantification of lignin and polyphenols in different organic materials • Nutrient monitoring (NUTMON) approaches introduced at two sites in West Africa • Optimum management for combined use of organic and inorganic resources established • Green manures and grain legumes do not work everywhere but there are niches where they could do well on-farm. • Grain legumes have a much higher likelihood for adoption by farmers due to their multiple benefits and often high profit margins. • High legume biomass and the consequent high amounts of N incorporated in soil do not always translate to high cereal crop yields because the soil systems are leaky though this can be minimised by manipulating timing of incorporation of the green manures. • Building on previous progress the modelling work this year involved revising the APSIM SoilN and MANURE modules so that the three fpools that comprise FOM can have different C:N ratios. • Similarly, following the work in Year 2 a similar approach was taken for the release of plant available P from organic inputs, so that P release depends on the pools having different C:P ratios. In Year 3 changes were also made to the APSIM SoilP and Maize modules to modify the uptake of P and its partitioning within the crop. • A linked soil-crop simulation model, ruminant livestock simulation model, household model and linear program module developed • Farm level crop-livestock integration scenarios in four countries developed • Extension of the preliminary dataset testing fertilizer equivalency value -organic resource quality relationships with data from the AfNet 2001 meeting and West Africa revealed that the original hypothesis put forward by Palm et al. (2001) are valid; the N fertilizer equivalency values were found to be linearly related to the N content of the organic resources for resources with a N content above 2.4% and the slope of the relationship between both characteristics was substantially lower for materials containing a large amount of soluble polyhenols. • Resource flow maps, drawn in various sites across East Africa, confirm the very diverse range of soil management options implemented by small scale farmers and point towards various potential options to improve the use efficiency of add organic and mineral nutrient sources for various farmer wealth classes and overall biophysical and socio-economic conditions. • Community meetings generated a baseline of \"folk ecological\" knowledge in four communities of Western Kenya, along a gradient from high population density Vihiga district, through Busia, to lower population density Teso district. Culturally this gradient also extends from predominantly Luyia (Bantu) to Teso (Nilotic) speakers. Considerable common local knowledge was identified and characterised in local reports. Local soil taxonomies are quite detailed, describing soil quality as a function of topsoil colour and texture, location within the topography, and the presence or absence of signs of degradation (erosion, excessive weediness or stoniness). Since soil 'fertility' is perceived only through indirect means, such as the presence / absence of certain indicator plants or the vigour of crop growth, it is usually conceived of in very holistic terms (i.e.: fertility, weed and pest dynamics are strongly interrelated in local vocabulary). One of the key knowledge gaps identified is that, while many farmers recognise various crop leaf discolorations as signs of 'low fertility' there is not widespread understanding of there being multiple different nutrients in the soil which could be affecting crop performance. Many of the older women, who are frequently the farm managers, were not aware of the different nutrients provided by different commercially available fertilisers.• Seminars held to share findings between the sites led to community-level mapping of the soil types and transect ground-truthing exercises to further refine and verify the local soil classifications, as well as to discuss examples of various forms of soil alteration through management or neglect. Older participants revealed than many of the soil types seen today are degraded forms of older soil types, whereas younger farmers assumed the soils of today had also existed in the past and that 'a soil cannot change itself'. The locally recognised diversity of soils is greater than that depicted on scientific soil maps of the study regions. As a result, local farmers complained that experimental plots for new technologies are often not situated on enough of the local soil types for people to draw inferences about where (or if) they would be appropriate. • Key informants have been selected and interviewed to gather their knowledge of soil fertility processes, indicators of soil fertility status changes, and the evolution of their soil management practices. Many older farmers' felt that they cannot really apply their knowledge of how to match crops with suitable soils or agro-ecological niches because land sizes today are too small, demands for annual maize production are relentless, and access to different niches is limited now that the landscape is fully settled. One unexpected finding has been that local knowledge of soil fertility is not any less amongst younger farmers, in part because those who have stayed in the area are those who by intent (or lack of alternative options) have a commitment to agriculture. They also tended to have better understanding of soil nutrients and of potential new technologies. • Multiple studies of the distribution and extent of knowledge on local indicators of soil fertility status and changes conducted in Teso, Busia, Siaya, and Kakamega districts conducted. • Ethnobotanical study of plant species indicating soil fertility status and changes initiated in Meru South and adjacent districts of Central Kenya as companion study to work conducted in Latin America. (MSc student) • Evaluation of local decision-making related to concepts of 1) high vs. low quality residues and 2) soil nutrients, using community-based demonstration plots in Western Kenya. Initial round of plots completed and follow-up activities with farmers in progress. • Farmers, extension, and KARI-Kakamega field staff were trained in participatory monitoring and evaluation methods. Several forms of farmer recording keeping were introduced in 2001 to monitor and evaluate progress with the soil fertility management technologies. However, lack of funds has limited follow-up, which has lead to widely varying levels of farmer interest and disparate standards of data collection. • A baseline survey of soil fertility management practices and socio-economic conditions was completed and analysed for 314 farmers in the West Kenya site. The methodology was shared with the Ugandan and Tanzanian sites. These data will now be compiled and analysed along with comparable studies conducted at the other BMZ project sites in West Africa (Togo and Benin) to produce a scientific paper relating soil fertility management practices to the contrasting socioeconomic and agro-ecological conditions of the sites. • A formal economic survey of on-farm use of organic and inorganic resources has been designed for the BMZ sites in Kenya, Tanzania, Uganda, Benin, and Togo, and will be implemented at the end of 2002 / early 2003. • Public and private benefits and costs of different ISFM options evaluated using the policy analysis matrix (PAM) technique. This approach is particularly useful for examining the role of transaction costs and market failures in influencing profitability of new technologies. (MSc student) • Evaluating whether the soil fertility management and livelihood enhancement needs of different classes of farmers are being met with the ISFM options currently available to them, by contrasting the profitability of different options (using gross margin analysis). (MSc student) • Evidence for the external constraints (such as the mis-functioning of input and output markets) on adoption and use of ISFM options documented and explained. For example, the bumper harvest reported in Kenya and Uganda in the 2001 short-rain season led to sale prices of maize that were often below production costs. In such situations, farmers face the prospective of losing money if they sell their maize to generate cash, but there is also no incentive for them to invest in their agricultural enterprises given the policy environment they operate within. Clearly, innovations need to address food security and livelihood sustainability, not just increased production as a good in its own right.Policy interventions that would rationalise input and output markets, and buffer smallholders from their volatility, should have as their goal a) increasing farmers' opportunities to innovate, and b) making investments back into agriculture attractive. (Paper presented to ILRI-IFPRI conference on \"Policies for Land use management in highland East Africa\".) • Review of African smallholder experiences with integrated soil fertility management practices found growing use, both indigenously and through participation in agricultural projects. Patterns of use vary considerably across heterogeneous agro-ecological conditions, communities and households.The potential for integrated soil fertility management to expand markets for organic inputs, labour, credit, and fertilizer explored. Markets for organic markets are hampered by inherent constraints such as bulkiness and effects on fertilizer markets are conceivably important, although no good empirical evidence yet exists on these important points. (Paper submitted to Food Policy for special issue on \"Input use and input markets in sub-Saharan Africa\") • Proposal submitted to FASID (Foundation for Advanced Studies in International Development) to examine the links between improved agricultural technologies and practices and productivity change and poverty reduction in smallholder communities and households. Agricultural technologies considered will include crop, livestock, and natural resource management innovations. Technological change is taken to be improvements in productivity of existing resources and enterprises (e.g. adoption of input packages leading to higher yields of crops) as well as the shifts in the composition of resources or enterprises (e.g. adoption of higher value added crops). • The changing theoretical and methodological approaches of integrating social science into TSBF's research activities over the past decade were examined, and strategic lessons relevant to INRM research identified. The interdisciplinary \"experiment\" of TSBF has steadily taken shape as a shared language of understanding integrated soil fertility management. While individual disciplines still retain preferred modes of conducting fieldwork (i.e.: participant observation and community-based learning for \"social\" research, replicated trial plots for the \"biological\" research) a more \"balanced\" integration of these modes is evolving around activities of mutual interest and importance, such as those relating to decision support for farmers using organic resources. Since TSBF is working constantly through partnerships with national research and extension services, it has an important role in stimulating the growth of common bodies of knowledge and practice at the interface between research, extension, and farming. To do so requires strong champions for interdisciplinary, collaborative learning from both natural and social science backgrounds, the commitment of time and resources, and patience. • The proportion of legumes in the farming systems is very low, and integration of legumes into system is constrained mainly by socio-economic factors • Legumes with multiple benefits were accepted by farmers than legume cover crops • The biophysical indicators used by farmers for selection were firm root system, early soil cover, biomass yield, decomposition rate, soil moisture conservation, drought resistance and feed value as important criteria. • The socio-economic indicators that dictated integration of legumes into systems were depended on land productivity, farm size, land ownership, access to market and need for livestock feed. • A draft decision guide was developed by combining the biophysical and socioeconomic indicators • The CIAT-TSBF Working Group prepared a position paper on \"BNF: A key input to integrated soil fertility management in the tropics\" as part of the Pre-Proposal preparation for BNF Challenge Program. • Case studies in Latin America show that there is a consistent rational basis to the use of local indicators of soil quality and their relation to improved soil management.• Initial plant quality parameters that best correlated with decomposion were neutral detergent fibre (NDF) and in vitro dry matter digestibility (IVDMD) could be useful lab-tests during screening of plant materials as green manures. • Green manures that decomposed and released N slowly resulted in high N uptake when they were used at pre-sowing in a tropical volcanic-ash soil. • When Tithonia diversifolia is to be used as a fallow species, the use of plantlets as compared to the stake method of establishment was associated with better for nutrient acquisition and use efficiency. • Annual application of high amounts of chicken manure can lead to surface sealing and crusting in volcanic-ash inceptisols in Colombian hillsides which is reflected in reduced water infiltration and air permeability and high superficial values of shear strength. • Extension of the preliminary dataset testing fertilizer equivalency value -organic resource quality relationships with data from the AfNet 2001 meeting and West Africa revealed that the original hypothesis put forward by Palm et al. (2001) are valid; the N fertilizer equivalency values were found to be linearly related to the N content of the organic resources for resources with a N content above 2.4% and the slope of the relationship between both characteristics was substantially lower for materials containing a large amount of soluble polyhenols. • Resource flow maps, drawn in various sites across East Africa, confirm the very diverse range of soil management options implemented by small scale farmers and point towards various potential options to improve the use efficiency of add organic and mineral nutrient sources for various farmer wealth classes and overall biophysical and socio-economic conditions.Output 2: Improved soil management practices developed and disseminated• Biological analysis of ISFM options conducted in collaboration with System wide Livestock Programme (SLP) • Participatory economic analysis of current ISFM options conducted at benchmark sites • Hill placement of small quantities of fertilizers evaluated at four sites on-farm • Establishment of credit systems to increase farmers' access to external inputs at one site in the Sahel of West Africa. • The APSIM model over-predicts the effects of fertilizer N only for the organic-inorganic N combinations and under-predicts release of nutrients from cattle manure. • Manure decision guides have been developed and tested with farmers in Zimbabwe Current efforts are being made to evaluate the usefulness of these guides as communication tools to enhance uptake of soil management options. • Farmers' categorizations of manure quality correlate well with laboratory indices and can be linked to use strategies of different types of manure. • A District co-ordinated and run soil productivity enhancement program established in Tororo District.More than 3000 farmers accessing new and improved sources of information and technology options. Project funded successfully raised for 2003-4. • Improved dual-purpose legume and improved fodder germplasm evaluated in Uganda • Legume cover crops and biomass transfer species for maize production in Uganda evaluated • Economic analysis of Legume cover crops and biomass transfer species for maize production in Uganda conducted • Updated and new extension leaflets produced • Studies of social capital and dissemination pathways completed • Impact of policy on land management options investigated.• Various dual purpose grain legumes are found to perform very well in terms of BNF and biomass production in Western Kenya. A certain level of variation in access to low available soil P between the various accessions was also noted.• Significant rotational benefits were observed on maize after both herbaceous and dual purpose grain legumes, but most of the time only when P had been applied to the legumes. A minimal amount of N fertilizer applied to the cereal following a legume led to equal or higher yields compared to the maizemaize treatment receiving a recommended dose of N fertilizer. • Twenty farmers participated in resource flow mapping (RFM) exercises in Emuhaya sub-location, the AHI/BMZ site in Western Kenya. The objective was to characterise their soil fertility management practices for the 2000-2001 cropping seasons. The participant selection was stratified on the basis of their wealth ranking in PRA's conducted earlier. Partial nutrient balances are in the process of being calculated using NUTMON. Initial results (from both West Kenya and work in Uganda) suggest that wealth class per se is not a good predictor of how well the soil will be managed. Higher wealth class households may use more externally purchased inputs, but their overall nutrient balances are also frequently lower than less resource endowed households. • Following visits by Emuhaya farmers to other regions of Kenya, local initiative has led to the creation of three farmer field schools. These groups have a broader membership than the original farmer research groups, and have stimulated considerable interest in soil fertility management using high quality manure, marketable vegetable crops (particularly kales) and improved maize and bean germplasm. • A community resource centre begun with the Ministry of Agriculture and Livestock Development (MOALD) in Emuhaya currently lacks materials. Renovation is to start after the long rains are finished (July/August 2002) and at this time community involvement will help develop the centre in directions that meet local needs. It is proposed that decision aids and other potential extension tools generated through local research will be disseminated and tested through this centre, to better understand the potential channels of information sharing. Links are also being explored with local NGO's active in soil fertility management (SCODP) and input traders and stockists in the private sector.• Principles for conducting research to integrate local and scientific understanding of soil fertility processes are being compiled for development of a Field Manual to support trainers, farmer leaders, and scientists. • Draft publication on the role of social networks in the generation and sharing of agricultural information has been submitted to the International Institute for Environment and Development (IIED) for inclusion in their Gatekeeper Series. • A decision support plot was planted in March 2002 in Emuhaya to demonstrate concepts of both a) resource quality and b) nutrient deficiency. Follow up meetings at top-dressing have generated some interest with farmers who have not previously taken part in research activities. However, a better effort at labelling and visually explaining the demonstration site will improve its potential to communicate.• Collective activities at the harvest evaluated which organic material classes should be considered 'high' quality and identified additional local or exotic materials that could be collectively tested on the plot next season. Recommendations on new experimental designs and site locations were made and will be incorporated in next season's activities, which will also include several of the 'folk ecology' project sites. • Land degradation in East African Highlands is at an alarming stage, and yet soil conservation practices are not well accepted as there the technologies did not participate the communities in decision making. • Four major steps were outlined to reverse the trend of land degradation namely, participatory characterization of the determinants of land degradation, community-led soil-water conservation practices, intensification of the system through integrated soil fertility management, and enhanced collective action to address communal resources. • Deep-rooted tropical pastures can enhance soil quality by improving the size and stability of soil aggregates when compared with soils under monocroping.• Increasing intensity of production systems resulted in improved soil physical conditions but decreased soil organic matter and macrofauna populations with the exception of agropastoral systems evaluated where a general improvement was observed. • Improved fallows with species such as Tithonia diversifolia under slash and mulch management can contribute to the rapid restoration of soil fertility that has been exhausted by continuous cassava cultivation with little or no inputs. • Determined the influence of contrasting agropastoral systems and related P fertilizer inputs on size of P fractions in soil and their isotopic exchangeability and showed that organic P dynamics are important when soil Pi reserves are limited. • Showed that the use of vertical tillage and agropastoral treatments can contribute to the build-up of an arable layer in low fertility savanna soils of the Llanos of Colombia as indicated by improved soil physical properties and nutrient availability.Output 3: Ecosystem services enhanced through ISFM• The first Phase (2002)(2003)(2004) of the project on 'Conservation and sustainable management of belowground biodiversity' (BGBD) was endorsed by the Council and Chief Executive Officer of the GEF for $5 million. TSBF-CIAT is the Executing Agency on behalf of partners in seven countries ie. Mexico, Brazil, Cote d'Ivoire, Uganda, Kenya, India and Indonesia. A successful start-up workshop was held in Wageningen in August, hosted by BOT member Ken Giller • TSBF undertook the quantification of biological nitrogen fixation using isotope dilution technique and samples are sent to IAEA in Vienna for 15N analysis. • The chemical analysis of samples from the long-term trials are in progress.• Yield from the long-term trials can be increased up to five fold when organics and inorganics are used in combination in a legume cereal rotation system. • The significant effect in a cereal rotation is not only due to the nitrogen effect of the legume but more on the change in biological soil properties. • Food deficit in Africa is not only the function of food shortage but also quality.• The barley-based systems offer a considerable quantity of calorie and zinc, but deficit in vitamin A and calcium. • It was possible to suggest a balanced human nutrition by reallocation of the land through optimization models using the existing resources. • Tropical Secondary forest regrowth following pasture abandonment in Central Amazonia rapidly sequesters C in the soil where there is greatest potential for long-term C gains. • The slash-and-char technique that involves charcoal additions to the soil significantly increased biomass production of a rice crop in comparison to a control on a Xanthic Ferralsol from the Central Amazon and opens new possibilities to enhance C sequestration in soils in areas where burning is a common management practice. • Introduction of improved pastures with deep rooting abilities can convert savannas from a net source to a net sink of methane. Soils under gallery forests, covering 10% of the area of the Colombian savannas, are responsible for 48% of total methane sinks. For a 20-year time horizon, the global warming potential of the Colombian savannas region under current land use distribution constitutes a very small fraction of estimated global planetary radiative contribution. • Agroforestry systems can lead to net C accumulation in soils close to total C stocks in the primary forest; however, charcoal derived-C found to 1 m depth can account for as much as 15% of total soil C and needs to be quantified when comparing the effect of land use change on soil organic C. Tanzanian universities and NGO's. Economists are the largest social science constituency interested in AfNET, but the input of sociologists, geographers, and anthropologists will also help broaden the relevance of ISFM research in the region • A participatory methodology has been developed that facilitates consensus building about which soil related constraints should be tackled first. Consensus building is presented as an important step prior to collective action by farming communities resulting in the adoption of improved soil management strategies at the landscape scale. • 12 field days were organized in Cauca and 3 training courses were organized in the Colombian Llanos. • Prepared and/or published 34 articles for refereed journals, 3 books, 18 book chapters and 15 articles for conference proceedings most of which which are coauthored with other institutional partners. • 72 students are associated with the project (24 Ph.D. theses).• Established and maintained collaborative links with NARS and ARO partners.Output 1. Biophysical and socioeconomic constraints to integrated soil fertility management (ISFM) identified and knowledge on soil processes improvedInformation related to N fertilizer equivalency values obtained in W-Africa has been added to the information obtained in E and S Africa and was presented during the Nitrogen meeting in September 2001 in France. Trials on the 'optimum management of low quality organic resources' were established in several sites in East and West Africa. The major objective is to determine the immediate and residual response of various cereals (maize, rice, sorghum) to application of low to medium quality materials (manure, maize stover, rice straw, etc) combined or not with various rates of urea. Preliminary results of the first season show significant responses to N in most of the sites and limited impact of the organic resources. The immediate response of these materials was rather neutral in most places, except in the Sahel where strong responses were observed. Currently, the first residual year is being determined. The trials also look at the P supply potential of various organic resources. Moderate applications of manure were observed for supply all the P required by a maize crop in Nigerian and W Kenya. This observation could not be explained in terms of amount of P supplied alone; effects on the P sorption dynamics are likely to have contributed to the observed effect.Trials on the 'optimum N and P management in legume-cereal rotations' were established in several sites in East and West Africa. The trials look at the impact of grain and herbaceous legumes on soil fertility status. Treatments are a herbaceous legume (most of the time Mucuna) and a grain legume (mostly soybean or cowpea) followed by maize with and without application of mineral N. The legume is treated or not with TSP, and significant responses were observed to P application in most sites.. The impact of previous legumes and application of P to the legume on the need for N and P of a subsequent maize crop will be evaluated.Trials on organic/mineral interactions are being established in 2 sites in Ethiopia (Ginchi, Mekelle). Legume species used include local species and improved varieties of 'exotic' species. The work in Mekelle is part of the PhD thesis of Kiros Habtegebriel, in Ginchi of Balesh Tulema.The NUTMON approach was introduced at two sites in Sadore (Niger) and Samanko (Mali) to install capacity to monitor nutrient inputs and outputs from cropping systems. The packages consist of two data collection questionnaires, which were translated into French for ease of use and the NUTMON Toolbox model kit. At both sites, the work was undertaken in collaboration with ICRISAT with funding support from the system wide livestock project. Data wil be analysed in 2002.Following the installation of NUTMON toolbox, farmer input and output inventory survey data has been collected in Mali and Niger. These data are now being logged into the model together with monitoring season data that are being recorded during the current crop and livestock activity season. The initial farm NPK balance output will be available later in 2002. In July 2002 the ICRISAT laboratory supervisor based at Sadore in Niamey visited TSBF for training in resource quality analysis. Currently, several samples of manure and crop residue input materials used in the 2002 season network trials have been analysed for %N, % Lignin, and % Polyphenols.Several network experiments were established at benchmark locations in different agro-ecological zones of West and East Africa to look at N fertilizer equivalencies of organics. The input materials are low quality cattle manures and crop residues (rice and maize stover). The most important research highlight is that whereas the fertilizer equivalency values of low quality manure were very poor in the Sub-humid and humid zones, their values were very high (>250%) in the semi-arid zones. Indicating that the critical value for immobilization and mineralization is site specific.• Soil, water, nutrient and knowledge constraints to sustainable production defined and the understanding of the role of soil biota, multipurpose germplasm, and organic and inorganic resources for sustainable management of land resources improved.Most of the work focussed on integrating legumes into farming systems looking at where some of the legumes could work best and analyzing the reasons why the perform best under the sets of conditions. Legume work was established in the 2000/01 season to screen legumes in two areas, Murewa (annual rainfall 800-1000mm) and Shurugwi (annual rainfall 600mm). Results in the first season showed that the three green manures, Crotalaria grahamiana, Crotalaria juncea and Mucuna pruriens produced high biomass and added higher amounts of N to the soil compared with the grain legumes, Vigna unguiculata and Glycine max. Of the green manures, Mucuna pruriens was found to give the highest biomass and N addition to the soil on some of the sites while Crotalaria grahamiana gave higher biomass yield and N addition to the soil at some of the sites. Biomass yields were as high as 6000 kg ha -1 with N addition of up to 200 kg ha -1 . Vigna unguiculata gave higher biomass and grain yields than Glycine max with biomass as high as 2000 kg ha -1 and 1000 kg ha -1 of grain. In the second season, Crotalaria grahamiana had the highest maize yields of up to 2000 kg ha -1 even on the sites where Mucuna had higher biomass yields in the first season. Early incorporation gave higher yields than late incorporation. There were however no treatment differences in maize yields because of the drought that was experienced during the season causing low yields.Experiments were setup in the 2001/02 season to establish the biophysical boundary conditions under which different legumes perform in Malawi, Zambia and Zimbabwe (Murewa and Shurugwi). Biomass yields of the legumes were generally high in the clay soils than in the sandy soils. The correlation between clay content and biomass yield was however poor probably because of the drought experienced causing moisture to be the most limiting factor. Similar trends were observed for pH, CEC, %C and available P content with biomass yields. Generally, higher biomass yields were observed for green manures than grain legumes in Zambia and Zimbabwe. There was crop failure in southern Zambia while biomass yields of up to 16 000 kg ha -1 of Crotalaria juncea were observed in the northern parts of the country where rainfall was high. This experiment will be repeated on the sites where biomass yields were low and maize will be planted on the sites where legume biomass yields were greater than 2500 kg ha -1 .A PRA was conducted with farmers from Shurugwi to establish how smallholder farmers prioritise legumes in their farming system, establish factors determining the area allocated to legumes, farmer perceptions on legumes and green manures introduced through farmer participatory research trials and identify opportunities for increasing the role of legumes and green manures in soil fertility management. Farmers identified legumes as the second major crop in the smallholder farming system after maize. Farmers grow legumes mainly for cash and food. More than 90% of the farmers were aware of the potential of legumes especially groundnuts in improving soil fertility. Diseases and limited land available limit opportunities for expanding area under legumes. Green manures were identified as very important in reclaiming poor soils were maize yield responses, even with fertility inputs, is limited and as fallow crops. Farmers identified green manures with multiple uses as more appropriate and likely to be adopted. Multiple benefits and labour requirements were identified as the most important criteria for identifying green manures for adoption. Farmers ranked mucuna highly for its potential in improving soil fertility, controlling weed growth, controlling striga, its use as a coffee bean and that it is easier to incorporate compared to sunhemp and crotalaria. On grain legumes, groundnut was ranked first as it is the main cash crop for most smallholder farmers. Soyabeans appeared to have a much higher likelihood for instant adoption by most farmers due to its multiple benefits and high profit margins. A benefit cost analysis revealed that cowpeas had the most attractive gross margin per hectare compared to the green manure legumes. The value of the other cowpea benefit as a relish, though very important, was not included in computing the margins per hectare. The Net Present Values (NPV) for all the green manures were negative. Cowpea had a positive NPV. The biomass produced by the green manures may not have been large enough to raise the fertility status of the soils to achieve the desired yield levels for maize in the second year.• Soil, water, nutrient and knowledge constraints to sustainable production defined and the understanding of the role of soil biota, multipurpose germplasm, and organic and inorganic resources for sustainable management of land resources improved.Soils at our Central America reference sites in Honduras and Nicaragua appeared to be both N and P limited thus responding best to the combined application of N and P. One Post-Doctoral Fellow started studies in Nicaragua on \"farm resource and nutrient flows\" in the Wibuse watershed at the San Dionisio Reference Site in Nicaragua following training with TSBF colleagues in Africa.In the hillsides of the Cauca department-Colombia, we made progress in the identification of some biophysical mechanisms that are related to crust formation. We found that excessive application of chicken manure as an organic fertilizer on Andean volcanic ash soils leads to soil crusting and sealing due to physical dispersion, chemical dispersion, and the interaction of soil physical and chemical characteristics.For the Llanos of Colombia, field studies conducted at Carimagua and Matazul (Savannas) contributed to define lime and nutrient requirements for acid soil tolerant varieties of rice, maize, cowpea and soybeans in rotational production systems on heavy-textured Oxisols. Field and glasshouse studies on crop and forage components indicated that forage legumes are more efficient in acquiring P per unit root length. Comparative studies of a forage grass (Brachiaria dictyoneura CIAT 6133) and a legume (Arachis pintoi CIAT 17434) demonstrated that the legume could acquire P from relatively less available P forms from oxisols of Colombia.For the Llanos of Colombia….The increasing attention paid to local soil knowledge in recent years is the result of a greater recognition that the knowledge of people who have been interacting with their soils for long time can offer many insights about sustainable management of tropical soils. Case studies show that there is a consistent rational basis to the use of local indicators of soil quality. Biological indicators (native flora and soil fauna) were shown to be important local indicators of soil quality related to soil management. Although benefits of local knowledge include high local relevance and potential sensitivity to complex environmental interactions, without scientific input local definitions can sometimes be inaccurate to cope with environmental change. It is argued that a joint local/scientific approach, capitalizing on complementarities and synergies, would permit overcoming the limitations of site specificity and empirical nature and allow knowledge extrapolation through space and time.Field research in Cauca showed that decomposition and nutrient release rates by green manures of contrasting chemical composition or quality were significantly correlated with initial quality parameters often used by animal nutritionists in the lab like neutral detergent fiber (NDF) and in vitro dry matter digestibility (IVDMD). This observation highlights potential usefulness of these lab-based measures as screening methods for large numbers of potential green manure materials in relatively short time. Glasshouse studies showed that at pre-sowing surface application of low-quality green manures (i.e. Calliandra calothyrsus) and/or surface application of high quality green manures (i.e. Indigofera constricta) during periods of high crop demand could be seen as alternative nutrient sources for hillside farmers cropping volcanic-ash soils. We also investigated the effects of establishment in Tithonia diversifolia, from bare root seedlings (plantlets) and vegetative stem cuttings (stakes), because this plant has the ability to sequester nutrients from soil in its tissues, including P, and has been shown to be useful for cycling nutrients via biomass transfer and improved fallow. Nutrient uptake efficiency (μg of shoot nutrient uptake per m of root length) and use efficiency (g of shoot biomass produced per g of shoot nutrient uptake) for N, P, K, Ca and Mg were greater with plants established from plantlets than those established from stakes (is it right). Improved nutrient acquisition could be attributed to relief from P stress and possibly uptake of some essential micronutrients resulting from mycorrhizal association.Field research carried out at Carimagua showed that both native savanna and introduced pastures develop deep root systems compared to field crops such as maize. Studies on root distribution of maize showed that most of the roots are in top 20 cm of soil depth. Application of higher amounts of lime did not improve subsoil-rooting ability of maize but contributed to greater nutrient acquisition. Cultivation with disc harrow (8 passes) markedly improved maize growth and nutrient acquisition. We made progress in demonstrating the importance of deep-rooted tropical pastures to enhance soil quality by improving the size and stability of soil aggregates when compared with soils under monocropping. The concepts and strategies developed from this work are relevant to different areas of the Llanos for improving soil quality and agricultural productivity.Resource flow maps were drawn calculated for various farms belonging to various wealth classes in Lushoto (Tanzania), Western Kenya, Iganga (Uganda), Mekelle (N Ethiopia), Ginchi (W Ethiopia), Hirna (E Ethiopia), and Areka (S Ethiopia). Currently the partial nutrient balances are being calculated and evaluated with the NUTMON toolbox. The partial nutrient balance calculations need to be completed and the impact of wealth and overall economic environment evaluated. Idea is to get a paper out on this topic in the framework of the current projects. Follow-up NUTMON data processing meeting in Addis (planned somewhere in February 2003).Preliminary relationships between OM Q and SOM characteristics are being investigated in existing medium-to-long term trials (Meru, Kabete, Nyabeda) where organic resources of varying quality have been applied (all trials contain Tithonia and Calliandra applications). The delta 13C technique will be used. The SOM status (quantity and quality) of soils will be related to a set of specific soil properties essential for proper crop growth in an attempt to 'valorize' SOM. The use efficiency of mineral fertilizer is being determined using 15N labeled fertilizer, in a set of treatments of the trials (control, Calliandra, Tithonia) to determine relationships between SOM status and fertilizer use efficiency. The soil properties to include in the evaluation work will be decided upon and preliminary relationships between SOM status and these properties will be evaluated. The samples from the microplots will be analyzed before the end of the year and preliminary N recoveries calculated. These activities are implemented through MSc projects of M Kirunditu and B Waswa. As set of trials looking at relationships between organic matter quality, environment, and soil organic matter quantity/quality have been established in Embu and Machanga (Kenya) and or going to be established near Kumasi (Ghana) and in Zimbabwe. Inputs are: Tithonia or Crotalaria, Leucaena of Calliandra, maize stover, sawdust, and manure. The organic resources are applied sole and in presence of N fertilizer. The trials are expected to run for at least 5 years. Further back-stopping of the trials in Kenya, Ghana and Zimbabwe.Output 2: Improved soil management practices developed and disseminatedIn 2001 TSBF was partner in the project of improving crop-livestock systems in the dry Savannah of West Africa with funding from the System wide Livestock Programme (SLP). The activities of this project were established in different sites in Nigeria, Niger and Mali. Most of the activities of TSBF were undertaken in Niger to evaluate on-farm best bet integrated soil fertility management following a rainfall gradient from 400 mm to 800 mm. The best bet options identified are the use of small quantities of fertilizers (4 kg P/ ha) hill placed at planting time, the combination of organic amendments such as manure and crop residue with mineral fertilizers and the increase of cowpea in the cropping systems due to the very positive effect of rotations of cowpea with cereals. The effect of crop residue use as mulch is more critical in the drier zone than in the high rainfall zone. Although the rotation of cereal with cowpea can double the succeeding cereal yield and cowpea is an important cash crop, farmers are not enthusiastic to adopt this option. The adoption of the hill placement of small quantity of fertilizer can double crop yields.Trials looking at the impact of cut-and-carry systems on nutrient balance were established in 2 sites (a poor and a fertile soil) in Nyabeda, Western Kenya in August 2001. Treatments are Tithonia, Calliandra, and natural fallow. The aboveground biomass production in these treatments is to be cut continuously and applied to a maize and kale crop. As such, the nutrient status under the shrubs and its effect on the quality of the aboveground biomass can be evaluated, together with the response of two important crops to organic matter application. Tithonia biomass production was about double as much on the fertile compared to the poor soil. No other data are available yet as the trials are just recently established. Evaluation of the response of maize and kale to the application of Tithonia and Calliandra residues.Trials looking at the impact of grain and herbaceous legumes on soil fertility status were established in East and West Africa during the first or second season of 2001. Treatments are a herbaceous legume (most of the time Mucuna) and a grain legume (mostly soybean or cowpea) followed by maize with and without application of mineral N. The legume is treated or not with TSP, and significant responses were observed to P application in most sites.Various classes of legumes (herbaceous, fodder, tree, grain) are being screened in Ginchi (W Ethiopia) and Mekelle (N Ethiopia) for their potential to accumulate biomass and supply N to the soil. No data are available yet as the trials are just recently established.Demonstration trials to evaluate with farmers the organic resource quality concept were established in BMZ benchmark villages. No data are available yet as the trials were just recently established (April 2002).A PhD student at the economic department of Purdue university used the field data collected to write his PhD dissertation with John Sanders and thesis is already published.Collaborative research continue with ICRISAT and FAO in Niger on the removal of the barriers to the adoption of soil fertility restoration technologies through the introduction of the Warrantage Credit Facility in the Sudano Sahelian zone and a progress has been prepared. The Warrantage Credit Facility was initiated to remove barriers to the adoption of soil fertility restoration. It provides access to cash credit to enable farmers to purchase external inputs such as fertilizers, while using storage of crops to enable farmers to get higher prices during the period when market supply begins to decline. In Karabedji, a village of western Niger, fertilizer consumption increased 10 fold from 350 to 3600 kg due to the warrantage system.• Relevant knowledge, methods and decision tools for improved soil management to combat soil degradation, increase agricultural productivity and maintain soil health provided to land users in the tropics APSIM was used to simulate manure technologies, improved manure storage and organic-inorganic N combination technologies. The model failed to simulate trends that were observed in the field for the improved manure storage systems. The model predicts high maize yields in the season of manure application for both high and low manures followed by yield decreases in the subsequent seasons, and this trend was true for high quality manure. The model under-predicts maize yields for high quality manure in the three seasons while for low quality manure the model over-predicts yields in the first season and under-predicts in the second and third seasons. In the field however, there were yields were low in the first season followed by yield increases in the second and third seasons for the low quality manure. For the organic-inorganic N combinations, APSIM over-predicts the effects of fertilizer N only. Manure decision guides have been developed and tested with farmers in Zimbabwe (see attached paper). Current efforts are being made to evaluate the usefulness of these guides as communication tools to enhance uptake of soil management options. There are, however, still a lot of issues that need to be covered to simplify the farmer decision guide and make it easier to use, for example determining the quality parameters and ranges for the different manures available to the farmers.A follow up PRA exercise was carried out to correlate the farmers' quality parameter and laboratory indices on manure quality three districts of Zimbabwe (see attached paper). This was followed by field trials with manures from different categories to test their effects on maize yield. Low maize yields were observed because of the drought, resulting in no treatment differences. Maize will be planted in the second season to test the residual effects of the manures. Use strategies were linked to manure quality however, there is need to explore these aspects further.Other farmer participatory experiments on manure and mineral fertilizer combinations, and soil moisture and nutrient conservation were not successiful. Differences in treatments on the trials were not observed due to the drought. Farmers expressed great interest in having these trials established again for the 2002/3 season.A logit model was used to analyse the determinants of adoption of pit storage system by smallholder farmers. The variables considered for the analysis were the age of the household head, number of cattle owned, educational status of the household, interaction of the household with extension agents, labour availability, experience of using manure and the total household income. Most of these factors were not statistically significant in explaining adoption of the pit storage system. The age of the household head and the number of cattle owned were significant in explaining the adoption of the pit storage system at 5%. Younger farmers were found to have a higher probability of adopting the pit storage system compared to the older farmers. This could be explained by the fact that young farmers have a lower risk aversion and that they are still able bodied and able to cope with the additional labour demands associated with pitting manure. Farmers with large heads of cattle had a lower probability of adopting pit storage system. Those farmers with larger heads of cattle were able to compensate for the poor quality of the manure by applying higher rates of manure per hectare. This could also be a reflection of the labour demands for storing large quantities of manure in a pit. The experience of using manure was statistically significant in explaining adoption at 10%. Farmers with experience using manure were in a better position to accurately assess the risk and returns of pit storing manure compared to heaping the manure.• Relevant knowledge, methods and decision tools for improved soil management to combat soil degradation, increase agricultural productivity and maintain soil health provided to land users in the tropicsThe concept of building an 'arable layer' has developed from the limited success of introducing intensive as well as no-till systems into acid-soil savannas in Colombia. In practice, this involves vertical tillage practices to overcome physical constraints, an efficient use of amendments and fertilizers to correct chemical constraints and imbalances, and the use of improved tropical forage grasses, green manures and other organic matter inputs such as crop residues, to improve the soil's \"bio-structure\" and biological activity. The use of deep-rooting plants in rotational systems to recover water and nutrients from subsoil is also envisaged in this scheme. Intensification of agricultural production on the acid-soil savannas of south America (mainly Oxisols) is constrained by the lack of diversity in acid (aluminum) tolerant crop germplasm, poor soil fertility and high vulnerability to soil physical, chemical and biological degradation. Out of a suite of croppings system options including monocropping, rotation with grain legumes, green manures and agropastoral systems compared with native savanna, only agropastoral systems (including maize/Panicum maximum+legume cocktail = Arachis pintoi, Centrosema acutifolium, Glycine wightii, Stylosanthes capitata) and rice/Brachiaria humidicola + legume cocktail,) were able to simultaneously improve the physical, chemical and biological properties of the soil.We investigated the effect of land-use systems and P fertilizer inputs on size of P fractions and their isotopic exchangeability. Differently managed Colombian Oxisols were labeled with carrier free 33 P and sequentially extracted after different incubation times. The recovery of 33 P in the two soils with annual fertilizer inputs and large positive input-output P balances indicated that resin-P i , Bic-P i and NaOH-P i contained most of the exchangeable P. The organic or more recalcitrant inorganic fractions contained almost no exchangeable P. In contrast, in soils with low or no P fertilization, more than 14% of added 33 P was recovered in NaOH-P o and HCl-P o fractions two weeks after labeling, showing that organic P is involved in short term P dynamics.In the Andean hillsides we have shown that an improved fallow with species such as Tithonia diversifolia in a slash and mulch system can contribute to the rapid restoration of soil fertility that has been exhausted after years of cropping with little or no inputs. Increased biomass production, greater accumulation and recycling of plant nutrients, especially phosphorus, with introduced fallow species are the reasons for the observed increases in soil fertility and biological activity. Tithonia has been shown to increase the pool of plant-available phosphorus.• The soils capacity to provide ecosystem services (global warming potential, water quality and supply, erosion control, nutrient cycling) and maintain soil biodiversity in the face of globl change in land use and climate enhancedIn a unique study for tropical savannas we have shown that the introduction of improved pasture species with deep rooting capacities can convert the agroecosystems of the savannas from a net source of global warming potential (total greenhouse gas emissions of carbon dioxide, nitrous oxide and methane) into a net negative potential or sink. The study is the first to collect data on all greenhouse gas emissions from different land management practices (cropping and pastures) and develop an overall global warming potential based on current and projected land use.• Research and training capacity of stakeholders in the tropics in the fields of soil biology, fertility and tropical agroecosystem management enhanced through the dissemnation of principles, concepts, methods and tools.A participatory approach in the form of a methodological guide has been developed and used in Latin America and the Caribbean (Honduras, Nicaragua, Colombia, Peru, Venezuela, Dominican Republic) and Africa (Uganda, Tanzania) in order to identify and classify local indicators of soil quality related to permanent and modifiable soil properties. This methodological tool aims to empower local communities to better manage their soil resource through better decision making and local monitoring of their environment. It is also designed to steer soil management towards developing practical solutions to identified soil constrains, as well as, to monitor the impact of management strategies implemented to address such constraints. The methodological approach presented here constitutes one tool to capture local demands and perceptions of soil constraints as an essential guide to relevant research and development activities. A considerable component of this approach involves the improvement of the communication between the technical officers and farmers and vice versa by jointly constructing an effective communication channel. The participatory process used is shown to have considerable potential in facilitating farmer consensus about which soil related constraints should be tackled first. Consensus building is presented as an important step prior to collective action by farming communities resulting in the adoption of improved soil management strategies at the landscape scale.• Optimum combination of organic and inorganic sources of nutrients In 2002, network experiments were conducted at 7 benchmark locations across 7 countries to investigate the nitrogen and phosphorus contribution of different low quality organic materials that are available for direct use by farmers. The sites include: Banizoumbou, Niger (Interaction of N, P and manure; Biological nitrogen fixation; Combining organic and inorganic plant nutrients for cowpea production); Maseno, Western Kenya; Kogoni, Mali; Farakou Ba, Kou Valley, Burkina Faso; Zaria, Nigeria; Kumasi, Ghana; Davie, Togo; Kabete, Kenya.• Fertilizer equivalencies of legume-cereal cropping For establishing equivalency of fertilizer value of legume-cereal cropping, experiments were established at Maseno in Western Kenya, Zaria in Nigeria, Kumasi in Ghana and Davie in Togo.Other aspects evaluated are Phosphorus (P) placement and P replenishment with Phosphate rock, Placement of phosphorus and manure, and farmer evaluation of soil fertility restoration technologies (Karabedji and Sadore). Abbreviations and acronyms: AABNF, african association for biological nitrogen fixation; AfNet, african network for soil biology and fertility; BNF, biological nitrogen fixation; CGIAR, consultative group on international agricultural research; CIAT, centro internacional de agricultura tropical; CNDC, combating nutrient depletion consortium; CP, challenge program; DSSAT, decision support system for agrotechnology transfer; ECABREN, eastern and central africa bean research network; FPR, farmer participatory research; FYM, farm yard manure; GIS, geographical information systems; ICARDA; ICRAF, international center for research on agroforestry; ICRISAT, international crops research institute for the semiarid tropics; IITA, international institute for tropical agriculture; INM, integrated nutrient management; INRM, integrated natural resource management; ISFM, integrated soil fertility management; MIS, integrated management of soils; NARS, national agricultural research systems; NGOs, nongovernamental organizations; OM, organic matter; ORD, organic resource database; PABRA, panafrican bean research alliance; PRGA, participatory research and gender analysis; Profrijol, Regional bean network for central America, Caribbean and Mexico; QTL, quantitative trait loci; SROs, specialized research organizations; SWNM, soil, water and nutrient management; TSBF, tropical soil biology and fertility; TSP, triple super phosphate; UNDP, united nations development program.It is widely recognized that biological nitrogen fixation (BNF) by the legume-rhizobium symbiosis is an important component of productivity in tropical agriculture, especially in farmland which is marginal either in terms of distance from the markets, or small farm size and poverty of the farmers. This position paper starts out by describing, the importance of BNF to tropical agriculture, the evolution of BNF paradigms, progress in creation of strategic alliances to combat soil fertility degradation, and past accomplishments of BNF-related research at CIAT-TSBF in collaboration with partners. Based on lessons learned, the paper suggests that BNF research should not be conducted in isolation but that a holisticmultidisciplinary-systems approach is needed to integrate BNF-efficient and stress adapted legumes into smallholder systems of the tropics. The paper proposes a number of research and development priorities for the BNF-CP to address for achieving improved contributions of BNF through integrated soil fertility management (ISFM) in the tropics. ISFM is the adoption of a holistic approach to soil fertility that embraces the full range of driving factors and consequences -biological, physical, chemical, social, economic and political -of soil degradation.BNF research on common bean and tropical forage legumes at CIAT started in the 1970s. CIAT maintains a collection of 5,628 Rhizobium strains. Lessons with BNF research in bean can be summarized as follows. BNF has not been a panacea, neither on the side of strain selection nor breeding of the host, but modest progress has been registered. On the one hand, even if response to inoculation is not dramatic, the technology is so inexpensive that any response at all is economically viable. On the other hand, the environment is at least as limiting on BNF as is the strain and the host. Therefore the benefits of BNF are best expressed in the context of an agronomic management system that addresses other components of the crop, especially phosphorus supply, drought stress and not infrequently starter N. Selection for BNF capacity under physiological stress has revealed genotypes (and possibly genetic systems) that are worth exploiting more fully and which could hold keys to broader progress. Research efforts on BNF in tropical forage legumes indicated that the main constraints to the widespread adoption of forage legumes include a lack of legume persistence, the presence of anti-quality factors such as tannins, variable Bradyrhizobium requirements and a lack of acceptability by farmers. The main problem identified is that there is not enough work done on participatory evaluation of legumes with farmers to identify their criteria for acceptability and feed this information forward into germplasm screening. What is needed here is better collaboration among stakeholders to really get legume adoption under way in the tropics.In other research programmes, it was confirmed that there is little scope for using legumes as an entry point to address soil fertility decline, but that there are various opportunities using multipurpose germplasm to indirectly improve the soil fertility status while providing the farmer with immediate products.TSBF researchers in collaboration with partners made substantial progress in creating an organic resource database and using it to construct a decision support system (DSS) for organic matter management based on organic resource quality. Analysis of organic resource data indicated a hierarchical set of critical values of nitrogen, lignin and polyphenol content for predicting the \"fertilizer equivalence\" of organic inputs. This decision tree provides farmers with guidelines for appropriate use of organic materials for soil fertility improvement. On-going TSBF network experiments are now addressing the organic/inorganic nutrient interactions to allow the refinement of the recommendations to farmers. TSBF and CIAT with a wide range of partners are also developing methods for disseminating ISFM options through processes of interactive learning and evaluation among farmers, extensionists and researchers.BNF-related research should proceed along the process-component-systems continuum and lead to demand-driven, on-farm problem-solving. Addressing farmers' problems in a systems context generates management options better suited to their local needs. Developing adoptable legume-BNF technologies to combat soil fertility degradation remains to be a major challenge. Research and development efforts are needed to integrate BNF efficient and stress-adapted grain and forage legume germplasm into production systems to intensify food and feed systems of the tropics. Several key interventions are needed to achieve greater impact of legume-BNF technologies to improve livelihoods of rural poor. These include: (a) integration of stress-adapted, BNF-efficient, grain and forage legume cultivars in rotational and mixed cropping systems, (b) development of management options aiming at optimal use of the legume-N in combination with strategic applications of mineral fertilizers to maximize Various BNF technologies addressinng the problems of food insecurity, poverty and land degradation can be identified with various potentials for BNF (Table 1). Legume-rhizobium symbiosis can sustain tropical agriculture at moderate levels of output, provided all environmental constraints to the proper functioning of the symbiosis have been alleviated (see later). Legumes can accumulate up to 300 kg N ha -1 in 100 to 150 days in the tropics (Table 1). Rhizobial inoculation in the tropics can enhance yield of grain legumes when phosphorus availability in soil is not a major limitation.Legume-cereal intercrops or rotations are widely practiced in the tropics to minimize the risk of crop failure and to provide households with improved diets. Traditionally, the main contribution of BNF in these systems is to improve household food security and human nutrition rather than improved soil fertility. Table 1, however, indicates various other niches for legumes in croppnig systems, each with their own specific contributions to improvement of food security, or land restoration.The African Association of biological nitrogen fixation (AABNF, 2001) summarized the first paradigm for BNF research of the 20 th century as \"the upper limits of BNF may be steadily increased by the collection and evaluation of ever-more effective N 2 -fixing micro organisms and their hosts because the distribution of this elite germless will necessarily accrue benefits following their introduction to production systems\". This paradigm during the 20 th century faced a major challenge that greater knowledge over time was not accompanied by improved BNF in the field. The widening gap between scientific advance of BNF and opportunities realized from their application is leading to the evolution of a new paradigm for BNF research. The 21 st Century Paradigm designed by AABNF for greater BNF impacts may be summarized as \"research in biological nitrogen fixation must be nested into larger understandings of system nitrogen dynamics and land management goals before the comparative benefits of N 2 -fixation may be realistically appraised and understood by society-as-a-whole\". It is critical to note that this assumption does not reduce the importance of nitrogen-fixing organisms and their products, but rather repositions them from a central auto ecological focus into a more integrated component of a larger, more complex task. The rationale behind this new paradigm is that it is not biologically-fixed nitrogen alone which sets the standard for successful contribution to social needs, but rather the products realized from more resilient and productive ecosystems that are strengthened through BNF.The former Tropical Soil Biology and Fertility Programme (TSBF), an international institution devoted to integrated soil fertility management (ISFM) research, has joined with the International Center for Tropical Agriculture to form the TSBF Institute of CIAT. This brings together TSBF's expertise in ISFM with that of CIAT in soils and land management as well as the complementary areas of germplasm improvement, pest management, GIS and participatory research. This merger builds on the strong collaboration between CIAT and TSBF in soil fertility research in East Africa that has developed within the CGIAR Systemwide Programme on Soil Water and Nutrient Management (SWNM) for which CIAT is the convening centre. ISFM is the adoption of a holistic approach to soil fertility that embraces the full range of driving factors and consequences -biological, physical, chemical, social, economic and political -of soil degradation. There is a strong emphasis in ISFM research on understanding and seeking to manage the processes that contribute to change. The emergence of this paradigm, very closely related to the wider concepts of Integrated Natural Resource Management (INRM), represents a very significant step beyond the earlier, narrower, nutrient replenishment approach to soil fertility enhancement. 1.4 Strategic alliance to combat soil fertility degradation through holistic approach (CIAT-TSBF-ICRAF)Soil fertility degradation has been described as one of the major constraints to food security in developing countries, particularly in Africa. Despite proposals for a diversity of solutions and the investment of time and resources by a wide range of institutions it continues to prove a substantially intransigent problem. The rural poor are often trapped in a vicious poverty cycle between land degradation, fuelled by the lack of relevant knowledge or appropriate technologies to generate adequate income and opportunities to overcome land degradation. Three international institutions, CIAT, TSBF and ICRAF, have joined together to form a strategic alliance, the goal of which is 'to improve rural livelihoods in Africa through sustainable integrated management of soil fertility' (Figure 1). The three partners have made significant contributions to combating soil fertility degradation over the past decade and have also a long record of collaboration through joint research projects. The alliance will go further however by building on existing networks and partnerships to implement a fully integrated programme of research and development activities. This triple alliance is regarded as the first step in a wider partnership consistent with the process of integration of international, and national, agricultural research activities. The ecoregional alliance, formed in 2000 by CIAT, ICARDA, ICRISAT and IITA, reinforces the regional and global dimension of the evolving research and development paradigm. The alliance represent a unique concentration of multidisciplinary expertise in legume research, with over 65 qualified scientists working on various aspects of legume production and utilization (genetic resources and breeding, agronomy and microbiology, plant protection, quality and post-harvest processing, and socio-economics). This ecoregional alliance sees achieving synergy in legume research as a key opportunity to make progress in improving food security, combating environmental degradation and alleviating poverty in developing countries. A BNF-CP would be an important axis of collaboration among the ecoregional alliance centers for all of whom legumes are a high priority. The BNF-CP would not, however, be the only area of collaboration on legumes research among the four centers. The ecoregional alliance will continue to explore other avenues for collaboration on legume genomics, adaptation to biotic and abiotic constraints, agroecosystem health, and rural innovation.1.6 Systemwide Program on Soil, Water and Nutrient Management (SWNM) SWNM is a systemwide global program of CGIAR created in 1996 to help multiple stakeholders rise to the challenge to reverse degradation of soils through the development of sustainable practices for managing soil, water, and nutrients. Operating through four complementary research consortia (combating nutrient depletion, optimising soil water use, managing sloping lands for erosion control, and integrated soil management), the SWNM program has developed a series of decision support tools and methodologies that are being tested across the different regions in Africa, Asia and Latin America covered by the program. SWNM program could serve as an important vehicle to test, promote and deliver BNFefficient legume technologies to improve rural livelihoods of farmers in the tropics.1.7 Systemwide Program on Participatory Research and Gender Analysis (PRGA)PRGA is a CGIAR systemwide program on participatory research and gender analysis for technology development and institutional innovation. The PRGA program develops and promotes methods and organizational approaches for gender-sensitive participatory research on plant breeding and on the management of crops and natural resources. PRGA is cosponsored by CIAT and three other CGIAR centers (ICARDA, CIMMYT and IRRI). A recent review carried out by the PRGA program found very little relevant experience in ISFM research with attention to gender-related needs or constraints (Kaaria and Ashby, 2001). This lack of a client-oriented, gender sensitive approach to the basic design of ISFM technologies has contributed not only to poor adoption but also to inequity. As a result the PRGA is currently supporting research to test novel approaches to pre-adaptive research for ISFM which are incorporating client-oriented participatory research methods, such as gender and stakeholder analysis, into very early stages of technology design. PRGA currently supports research on gender-differentiated approaches to developing technology for integrated nutrient management being conducted by CIAT's parricipatory research team. Linking BNF-CP with PRGA program could markedly enhance the ability to develop appropriate and adoptable legume technologies in the tropics. PRGA, together with ICRISAT, conducted a study on impact of participatory methods in the development and dissemination of legume soil fertility technologies and identified lessons that will be useful in BNF work (Snapp, 1998;1999a, b;Snapp et al., 2001;Johnson et al., 2001). TSBF is a partner in implementation of a subsequent project on the use of participatory approaches in research on natural resource management to improve rural livelhoods for women farmers in risky environments.BNF research at CIAT started in the 1970s. Several scientists including Peter Graham, Judy Kipe-Nolt, Douglas Beck (Beans) and Dick Date, Jack Halliday, Rosemary Bradley, Richard Thomas (Tropical Pastures) and others made significant contributions to developing practical ways to enhance BNF in legumes. CIAT maintains a collection of Rhizobium strains of 5,628 strains.BNF research in common bean (Phaseolus vulgaris L.) has spanned the range of strain selection, host improvement, and agronomic management, and recently QTL (quantitative trait loci) studies have been initiated (Graham, 1981;Graham and Temple, 1984;Kipe-Nolt and Giller, 1993;Kipe-Nolt et al., 1993). Thus, the case of bean illustrates both some of the successes and failures of BNF research. An important attribute of common bean, justifying its inclusion in low input systems, is the ability to fix atmospheric N and thereby reduce the depletion of soil resources. Beans in tropical environments are capable of fixing from 50 (CIAT, 1987) to 80 kg N ha -1 (Castellanos et al., 1996). Yet, actual N 2 fixation in bean cultivars is generally low when compared with many other grain legumes. Early research in the late 1970s indicated that this poor BNF is not due to an intrinsic inability of beans to nodulate because profuse nodulation can occur in controlled conditions in the greenhouse and in some soils. Although poor nodulation is frequently observed, soils in most bean growing areas contain large numbers of compatible and effective rhizobia. Selection of adapted Rhizobium strains for beans sown directly in pots of soils environmental constraints. CIAT has developed a group of 20 strains transformed with the gus gene while maintaining the symbiotic and competitive characteristics of the wild type. These genetically modified strains could serve as valuable tools to evaluate competition x environment interactions.Another valuable tool that was developed in 1990s was a series of non-nodulating lines. Mutagenesis was employed to create a mutant with a total lack of nodules. The non-nodulating gene in turn was backcrossed into a series of elite lines, to have at hand a ready tool for estimating the amount of nitrogen fixation in any given situation, by comparing non-nodulating and wild type paired lines.In summary, lessons with nitrogen fixation in bean can be summarized as follows. BNF has not been a panacea, neither on the side of strain selection nor breeding of the host, but modest progress has been registered. On the one hand, even if response to inoculation is not dramatic, the technology is so inexpensive that any response at all is economically viable. On the other hand, the environment is at least as limiting on BNF as is the strain and the host. Therefore the benefits of BNF are best expressed in the context of an agronomic management system that addresses other components of the crop, especially phosphorus, drought and not infrequently starter N. Selection for BNF capacity under physiological stress has revealed genotypes (and possibly genetic systems) that are worth exploiting more fully and which could hold keys to broader progress.BNF research on tropical forage legumes initiated in the late 1970s and continued throughout the 1980s and 1990s (Date and Halliday, 1979;Sylvester-Bradley et al., 1983, 1988, 1991;Sylvester-Bradley, 1984;Thomas, 1993Thomas, , 1995;;Thomas et al., 1997). Taking into account the wide range of forage legume genera being evaluated, about which very little information concerning BNF was available, the main priority was initially to determine need to inoculate. After improving the methodology for evaluation of need to inoculate, specifically by ensuring that the presence of mineral N was not interfering with the evaluations, by using different methods to immobilize mineral N, it was found that a surprisingly large proportion of the legumes showed responses to added N. This indicated that the naturally occurring rhizobial populations were inadequate, either numerically or in nitrogen fixing capacity under the given soil conditions. A program was developed whereby rhizobium strains which a) were able to compete with the native rhizobial population and b) would be effective on as wide a range of legume species as possible, were selected. A new method for strain selection, viz. the screening of large numbers of strains in undisturbed soil cores, was developed, and proved to be highly successful. Many statistically significant responses to rhizobial inoculation in the field were obtained.With funding from the UNDP, a network of scientists was established in the mid 1980s to evaluate legume-rhizobium symbioses in 14 countries of Latin America. The findings of this network were brought together at a workshop held at CIAT in 1987 where appropriate strain recommendations were made, and continue to be revised as a result of field evaluation by network members. The proceedings of this workshop were published in 3 volumes entitled \"The legume-rhizobium symbiosis, proceedings of a workshop on evaluation, selection and agronomic management\". A list of recommended Rhizobium strains for herbaceous and shrubby legumes is available. In addition, a manual of methods for legume-rhizobium studies plus an accompanying audio-visual package has been available for interested researchers in national programs.The marked responses to rhizobial incoulation observed in these trials led to the realization that a new way of inoculating the seeds of legumes was needed, so that the technology would be more available to farmers. In view of the fact that vaccines for both humans and animals are vital in tropical countries, and that the infrastructure for making them available is being developed in many areas, it was considered that this technology might also be useable for rhizobial strains. Traditional peat-based inocula need a large refrigerated storage space, and even if stored under refrigeration have a shelf life of only 6 months. Several different strains of rhizobia are needed for the different legume species being selected for pasturebased production systems, which complicates even further the possibility of supplying good quality traditional peat-based inoculants to farmers. CIAT therefore initiated a project to develop freeze-dried rhizobial inocula, also with funding from the UNDP. This project demonstrated that such inocula could survive for several years in vacuum-sealed vials, and that they can be suspended in water and applied to the seeds with high survival rates. This technology could well be a realistic alternative for supplying forage legume seeds and rhizobial inocula to farmers.Work on quantification of N 2 fixation using 15 N dilution studies was carried out in the late 1980s through a Swiss Development Corporation funded project (Cadisch et al., 1989(Cadisch et al., , 1993)). This project demonstrated the need to maintain adequate levels of both P and K for legume-based pastures that rely on biologically fixed N to supply the N requirement of the pasture.CIAT researchers were also the first to demonstrate fungal/bacterial inhibitory role of Bradyrhizobium strains isolated from tropical forage legumes (Kelemu et al., 1995). Screening of 15 strains of Bradyrhizobium from CIAT collection with in vitro tests showed that Bradyrhizobium can inhibit mycelial growth, reduce or prevent sclerotial formation, and inhibit sclerotial germination in Rhizoctonia solani. In addition, cell-free culture filtrates of three strains of Bradyrhizobium had inhibitory effects on the growth of the bacteria Escherichia coli and Xanthomonas compestris. The antifungal/antibacterial property may increase the competitiveness of Bradyrhizobium strains and enhance the chance of nodule occupancy and other beneficial responses with compatible forage legumes. Further research is justified to determine the impact of Bradyrhizobium strains on integrated disease and pest management in crop-livestock systems.As the objective of selection for improved N 2 fixation was mostly achieved, research in 1990s broadened from N 2 fixation per se to the role of the legume and N in productive and sustainable pasture and crop-pasture systems (Thomas, 1992;1995). This work showed that tropical forage legumes have the capacity to meet the requirements to balance the N cycle of grazed pastures. It also showed that the actual amounts required could depend on the rate of pasture utilization and the efficiency of recycling via litter, excreta and internal remobilisation. The efficiency of N 2 fixation (%of legume N derived from fixation) was found to be usually high in tropical pastures (> 80%) and is unlikely to be affected by inorganic soil N in the absence of N fertilizer application. This work resulted in a recommendation that an estimate of the amounts of N fixed by tropical forage legumes could be obtained from simple estimates of legume biomass provided tissue levels of P and K are adequate for plant growth.In an on-going and long-term crop-pasture rotations experiment in tropical savannas of Colombia, N dynamics were studied under cereal monocultures and rotations with greenmanure legumes with the objective to determine the use efficiency and fate of N derived from inorganic and organic sources (Friesen et al., 1998). Results indicated that N recovery by crops from residues was low (7-14%) while recovery from fertilizer was far greater (26-50% in biomass). Sequential measurements of soil profile mineral-N concentrations indicated a large accumulation of nitrate content to 1-m depth through the dry season and substantial nitrate movement through the soil profile during the wet season under both rotations and monocultures. Thus in a high leaching environments of humid tropics, poor N supplydemand synchrony can result in substantial leaching of nitrate below the crop rooting zone and eventual contamination of the ground water. Use of deep-rooted crop, forage and fallow components could minimize N losses from legume-based systems in the tropics.It was realized that the main constraints to the widespread adoption of forage legumes include a lack of legume persistence, the presence of anti-quality factors such as tannins, variable Bradyrhizobium requirements and lack of acceptability by farmers. But \"lack of legume persistence\" is not really a limitation if the seed is cheap enough. The legume seed can be broadcast into an already established pasture. Seed can cost as little as $4/kg and only 3 kg of Stylosanthes are needed per hectare. The problem is that there is not enough work done on participatory evaluation of legumes with farmers. What is needed here is better collaboration among stakeholders to really get legume adoption under way in the tropics.In areas where access to adequate quantities of mineral fertilizers is beyond the reach of low resource endowed farmers, organic sources of nutrients of animal and plant origins e.g. legumes will continue to be a critical source of nutrients (Palm et al., 1997). Organic materials influence nutrient availability (i) by nutrients added, (ii) through mineralization-immobilization patterns, (iii) as an energy source for microbial activities, (iv) as precursors to soil organic matter, and (v) by reducing the P sorption of the soil. The TSBF-SWNM (CNDC) organic resource database (ORD) with over 2000 data entries has been used to construct a decision support system (DSS) for organic matter management based on contents of nitrogen, polyphenol and lignin. Most studies indicated a linear response between N content and fertilizer equivalency values (FEQ) of the material with an increase of 8% FEQ for every increase of 0.1% N. In a recent study on evaluating FEQ of Tithonia diversifolia, Tephrosia, Sesbania and pigeon pea, yield increases up to 48% were recorded. This decision tree provides farmers with guidelines for appropriate use of organic materials for soil fertility improvement. On-going TSBF network experiments are now addressing the organic/inorganic nutrient interactions to allow the refinement of the recommendations to farmers. A systematic framework for investigating the combined use of organic and inorganic nutrient sources includes farm surveys, characterization of quality of organic materials, assessment of the FEQ value based on the quality of organics, and experimental designs for determining optimal combinations of nutrient sources. The desired outcome is tools that can be used by researchers, extensionists and farmers for assessing options of using scarce resource for maintaining soil fertility and improving crop yields (Palm et al., 1997). With the recent success of CIAT scientists with their partners in linking of the DSSAT crop models with the CENTURY soil organic matter model (Gijsman et al., 2002), the nutritive value of organic substrates for crop production can be analyzed under a range of climatic and soil conditions and for many different crops. The combined DSSAT-CENTURY also proved to be an excellent tool for evaluating the SOM pattern under low-input systems.A combination of resource flow mapping, ORD and FEQ has helped farmers to identify options for enhancing farm productivity and sustainability. Analysis of organic resource data indicated a hierarchical set of critical values of nitrogen, lignin and polyphenol content for predicting the \"fertilizer equivalence\" of organic inputs. TSBF and CIAT with a wide range of partners are also developing methods for disseminating ISFM options through processes of interactive learning and evaluation among farmers, extensionists and researchers.The potential for legumes is increasing for many smallholder farming systems in Africa as soil fertility declines and livestock management is intensified (Wortman and Kirungu, 2000). These two researchers summerized lessons from several cases where legumes have been promoted for soil improvement or forage. The cases included Mucuna in Benin, Sesbania and Tephrosia in Zambia, Calliandra in Kenya, improved fallows and green manures in Rwanda, Stylosanthes in west Africa, Tephrosia in eastern Uganda, best-bet niche options in central and eastern Uganda, and Lablab in western Kenya. These cases included those where the practice was well adopted by farmers, as well as cases of unconfirmed promise, and adoption failure.Over 15 years of work in West Africa with leguminous trees in alley cropping systems and Mucuna cover crops has led to a series of conclusions. First of all, such systems are technically sound and do maintain crop yields at substantially higher levels than traditional cropping systems. However, their adoption by farmers is relatively low or absent because (i) the appropriate niches for such systems were not properly identified (e.g., alley cropping must be targeted to high population density areas where firewood is needed and fertilizer is not easily available) and (ii) resource poor farmers require immediate benefits besides improved soil fertility.As a result of above developments and maybe due to the existence of crop improvement and resource management programs in the same institute, dual purpose grain and fodder legumes have been developed at IITA which improve the soil fertility status besides providing grains and fodder. Such legumes usually have a large proportion of N derived from the atmosphere, a low N harvest index and produce a substantial amount of above ground biomass. Residual effects on a cereal crop are often dramatic and fertilizer use to a subsequent cereal can be cut by 50% while still producing similar maize yields as a fully fertilized maize crop. Furthermore it was found that, e.g., soybean and cowpea could be false hosts for Striga hermonthica. One dual purpose soybean variety, TGX-1448-2E was specifically appreciated by farmers in Northern Nigeria, who commented that this variety yields more, produces more biomass than their own varieties. In addition, their succeeding maize/sorghum crops gave good yields with less N fertiliser than they would normally apply. The highest net benefits for the two seasons (1450 US$) were obtained with the rotation of TGX 1448-2E followed by the local variety Samsoy 2 (1000 US$). The lowest net benefits (600 US$) were obtained with lablab (Sanginga et al. 2001).From our past achievements, it is clear that BNF can contribute directly to the needs of a growing crop or can be added to the soil so contributing to its fertility. For sustainable agriculture in the tropics, there are two options: inorganic N fertilizers and BNF technologies that are less dependent on external purchased inputs. Approaches relying purely on external inputs are not often feasible, particularly for resource-poor farmers of the smallholder systems. In Africa, where the price of inorganic fertilizers is several times higher than world price, alternatives to inorganic fertilizers are especially important. A consensus has emerged that systems of ISFM are the only way forward, and it is in this context that we must consider the inputs from BNF (Figure 2).Decision by farmers to adopt ISFM is influenced by (and influences) a range of factors which can be grouped in 4 main dimensions, biophysical, economical, social, and policy (Kaaria and Ashby, 2001). The biophysical dimension influence on farmers include the basic characteristics of the BNF technologies as well as the overall quality of the resource base. The main economic factor that influences whether farmers practice ISFM is whether the economic benefits outweigh the costs, especially in the short run. ISFM/BNF technologies are often labor intensive and if labor costs are too high-or come at the wrong time of the year when farmers are busy with other activities--then farmers can not profitably adopt the technologies. Often labor-intensive practices like ISFM are only profitable when used with high value commercial crops. Social dimension also influence adoption and impact of ISFM. Where crop production responsibilities (and rights) are gender specific, ISFM technologies need to be consistent with these, e.g., appropriate for women work schedules or don't add additional labor for women when men get the benefits. Legumes can have important human health benefits, although care must be taken to assure that foods are properly prepared (e.g., mucuna) and culturally appropriate (if people won't eat them then may be can use as animal feed). Finally, a supportive policy environment is key to achieving widespread adoption. Fertilizer prices should be rational (not subsidized or taxed) and reflect real costs. This is the best way to ensure that farmers use the right combinations of organic and inorganic soil fertility management practices in their technologies. In addition, property tenure security is important to realize benefits of long-term investments, land ownership or long-term rental/use arrangements are important. Infrastructure investments such as roads and communications that open up marketing opportunities can help make adoption of ISFM profitable. Legume BNF can be a key input to ISFM. When legume BNF technologies are appropriately designed taking into consideration the incentives provided by each of these four dimensions, they could have positive impacts in each dimension as well. Legume-BNF technolologies can improve the sustainability of crop-livestock systems (biophysical), improve profitability, contribute to improved nutrition and gender equity (social). At the marco level, increased use of legume-BNF technologies could reduce use of costly imported inorganic fertilizers (policy).Most tropical soils have low inherent fertility and exhibit a variety of edaphic and climatic constraints including water stress, nutrient deficiency, low organic matter, and high erodibility. Inadequate soil and crop management has exacerbated these problems to an alarming extent. As a result of insufficient levels of nutrient replacement for that taken in harvest and other losses, high negative nutrient balances are commonly reported, particularly in sub-Saharan Africa.Intensification of agricultural production on smallholdings is required to meet the food and income needs of the poor, and this cannot occur without investment in soil fertility. Investing in soil fertility management is necessary to help households mitigate many of the characteristics of poverty, for example by improving the quantity and quality of food, income, and resilience of soil productive capacity. The effects of soil fertility degradation are not confined to the impact on agricultural production. The living system of the soil also provides a range of ecosystem services that are essential to the well-being of farmers and society as a whole.BNF-related research should proceed along the process-component-systems continuum and lead to demand-driven, on-farm problem-solving. Given the diversity of N 2 -fixing organisms, symbioses and habitats in which these organisms operate and the wide application and demand for fixed-nitrogen, BNF studies are by definition multi-disciplinary. Under the first paradigm for BNF research, microbiologists, plant physiologists and agronomists recognized the need for collaboration to respond to challenges posed by better management of nitrogen fixation, and now is the time to recognize the additional strengths derived from expanding this collaboration into wider interdisciplinarity as a means of better translating research findings into social benefits. Systems approach includes the involvement of stakeholders to finetune problem definition, the research itself, and the implementation of results. Stakeholders are farmers and citizens on farm and community levels, and policy makers and planners at higher level of aggregation.A comprehensive systems approach could be a necessary condition for the development of innovative, BNF-efficient, legume-based sustainable systems of the future. A programme of work must build on and use methods that have already proved successful and also develop and borrow others where significant gaps in understanding or application occur.The implementation of ISFM strategies on farms is likely to make the biggest contribution to agricultural sustainability in the tropics during the coming decade. When combined with robust, highly productive crop varieties, it is not uncommon for such systems to double yields in farmers' fields. The use of improved varieties is an integral part of the ISFM approach; ISFM is a specific strategy under the overall INRM research framework that aims at lifting the borders between crop improvement and natural resource management. A vital aspect of these strategies is the incorporation of farmers' indigenous knowledge at an early stage of systems development to enhance the adoption of ensuing technology.Considerable evidence exists that farmers have accumulated knowledge relevant for agronomic management (Carter and Murwira, 1995;Murage et al., 2000). Encouraging as this is, increasing land degradation, including often substantial soil fertility decline, suggests that locally devised methods, on their own, are no longer effective enough to cope with rapidly changing pressures on farmers (Johannes and Lewis, 1993;Pinstrup-Andersen and Pandya-Lorch, 1994;Murdoch and Clark, 1994).Farmers generally possess a vast body of knowledge about environmental resources in their farms but this knowledge is largely based on observable features (Talawar and Rhoades, 1998) rather than generalized knowledge. There is a general lack of process-based knowledge about agro-ecosystem function which is needed to cope with change, especially since much of it is unprecedented (i.e. climate change). This is in particular true for colonist farmers (Muchagata and Brown, 2000). In essence, lack of knowledge creates uncertainty that obstructs sound decision-making under conditions of change. This uncertainty about agro-ecosystem function prevents farmers from taking decisions that are too risky, and may have contributed to their reputation of being risk-averse. However, recent research points out that scientific knowledge can reduce farmers' decision-making uncertainty by enhancing local knowledge (Fujisaka, 1996). Some examples already exist that show how this can have positive synergistic effects for agro-ecosystem management (Steiner, 1998;Norton et al., 1998;Robertson et al., 2000).A holistic systems approach of ISFM is needed to address the smallholder to medium scale farming sector throughout the diverse agroecological zones of the tropics. This systems approach does not exclude process and molecular studies, but rather suggests that these tools be focused upon recognized constraints within farming systems. Research efforts on legume-BNF related aspects thereby become tools toward larger purpose, particularly in achieving food security and improving the diets of poor people in the tropics.Environmental factors affect BNF via growth and development of the host plant, the bacteria and also the process of interaction between the symbionts from the time of infection through the development of the nodules to the production and transport of products. The identification of the processes that are most sensitive to environmental constraints promises the greatest success in breeding programs or in an improvement of agronomic practices (Rao, 2001). The major environmental factors affecting BNF in the tropics are drought, soil acidity, soil nutrient deficiency and soil salinity. As substantial genetic variability in tolerance to most environmental constraints exists in both host legumes and rhizobial strains (Hungria and Vargas, 2000), there is potential for breeding and selection for improved genetic adaptation.Significant gains in impact can be achieved in the short to medium term by taking advantage of the huge legume and Rhizobium gene banks in participatory field evaluation and identification of stress-adapted legumes to specific ecological niches.Drought: It was recognized that drought affects BNF in legumes significantly. Decrease in soil moisture causes a rapid decline in the numbers of rhozobia in soil. However, Bradyrhizobium strains are more tolerant of desiccation than strains of Rhizobium over short periods (Bushby and Marshall, 1977). Rates of N 2 -fixation by legumes are more sensitive to reductions in soil moisture content than other processes such as photosynthesis, transpiration, leaf growth rates or nitrate assimilation (Serraj et al., 1999). Ureide-exporting legumes with determinate nodules appear to be more sensitive to drought than amide-exporting legumes (Serraj et al., 1999).Given the expansion of drought at an alarming state, especially in sub-Saharan Africa, and the need for incorporation of legumes into systems to improve soil fertility, there is a real need to improve the drought resistance of nitrogen fixing legumes. Although challenging, there is an opportunity to improve drought resistance using the existing genetic diversity and available tools in genetic engineering. CIAT has been working on development of drought resistant bean varieties, and identified resistant materials like BAT 477, to be used as genetic sources. A drought protocol was also recently developed for improvement of the genetic adaptation of beans in Africa (Amede et al., 2002). A possible strategy in the short-term could be improving water-holding capacity of tropical soils by increasing soil organic matter content and rate of water infiltration while reducing run-off and soil erosion. As most grain legumes in the tropics are grown as intercrops or relay crops, selecting best companion crops and adjusting the planting dates could minimize water stress effects on BNF.Soil acidity: Soil acidity is expanding in the humid and sub-humid tropics, mainly caused by improper land use and high rainfall intensity that encourage leaching of cations. Effects of soil acidity and the associated Al (aluminum) toxicity and P deficiency on BNF could be minimized through increasing the rhizosphere pH. One immediate option is liming but this is beyond the reach of resource-poor farmers, particularly in Africa. There is a consensus that continuous cultivation of legumes over longer time could lead to soil acidification. Therefore, crop rotation or intercropping legumes with cereals (maize-bean or sorghum-cow pea) is one sustainable strategy to improve BNF. Moreover, there are some tropical legumes that produce root exudates (mucilages & organic acids) that could minimize the effects of soil acidification through complexing Al ions. Other potential strategy is to identify legumes less sensitive to Al toxicity. Bean researchers at CIAT are breeding for improved Al resistance. ECABREN bean network in Africa has identified bean materials that are less sensitive to Al toxicity when grown under acidic soils of Democratic Republic of Congo. CIAT researchers in collaboration with NARS partners have selected a number of tropical forage legumes with very high adaptation to acid soils of the tropics (Rao, 2001).Soil nutrient deficiency: As mentioned earlier, the most limiting nutrient for BNF is known to be P, which becomes limiting in most tropical soils not only for legumes but also for all other crops. The P deficit in soils of the tropics is the result of combined effect of low inherent P content, very high P fixation, and limited application of soluble P (Rao et al., 1999). Some legumes (e.g. pigeonpea, chickpea) are much more efficient in utilizing P in P-fixing soils, mainly through release of organic acids that increase its availability. Moreover, ECABREN of CIAT identified bean materials that are performing well under low N, low P and low pH soils of Eastern & Central Africa, indicating genetic difference in nutrient use efficiency. Other institutes are working with P efficient cowpea and soybean (Sanginga et al., 2001).Soil salinity: Legumes that are grown in the drought-prone environments of sub-Saharan Africa, with saline or sodic soils, are commonly exposed to salt stress. Soil salinity could affect BNF through induction of water stress, pH effect, direct effect of Na ions or a combined effect. However, the rhizobia were found to be more tolerant than the host plant. Since the initial effect of slat stress is commonly expressed as water stress, improving the soil water availability would improve salt resistance of both grain and multipurpose legumes. Another strategy is integration of well-adapted N-fixing perennial legumes to reduce soil pH through acidification.As indicated before (section 2.1) one of the bred lines of beans, BAT 477, is not only BNFefficient but also well-adapted to major abiotic stress factors such as water stress and low P availability in soil. What is the probability that independent genes control tolerance of BNF to different stresses; that still other genes control BNF in stress-free environments; and these have come together in one genotype without any conscious selection? This is unlikely. Rather, the same genes probably confer high BNF under all these conditions. In this case, what mechanism could explain the tolerance of these genes to at least two stress factors? The genes of BAT 477 may be regulatory genes that are less sensitive to an internal stimulus that results in down-regulation and are thus less active in regulating BNF. Thus they confer high BNF under a wide range of conditions. It is significant that some QTL, which were tagged in BAT 477 under low P stress, also contributed to better BNF in the high P supply, suggesting that the corresponding alleles in DOR 364 (less adapted to low P supply) may not be expressed fully, even in optimal environments. Could gene regulation therefore limit BNF under optimal conditions? This hypothesis represents a different perspective on what restricts BNF in common bean. There is a need to investigate to what extent the poor BNF of common bean in fact reflects internal limitations of gene regulation.Legumes do occupy space and time in cropping systems and consequently, suitable temporal and spatial niches need to be identified within farming systems for widespread adoption by the farmer community. Temporal niches are defined by sequential or simultaneous occurrence of legumes while spatial niches are defined by the optimum location to plant legumes, based on farmers' production objectives. The latter often include under-utilized spaces on farm such as field boundaries, contour strips, or degraded fields. Snapp et al. (1998) identified six temporal niches for legumes. Spatial niches are also related to the existence of within-farm soil fertility gradients, created by inherent soil properties but more often by deliberate land management by the farmer. Such gradients are very often linked to farmers' wealth, and the overall socio-economic environment (e.g., access to input and output markets, credit schemes for intputs, etc.).Even nutrient use efficient and promiscuous legume germplasm requires proper crop management for optimal contributions of BNF. To alleviate P constraints to BNF, the simplest option is to apply soluble P fertilizer. In absence of such resource, another possible strategy is through application of rock phosphate. Preliminary evidence shows that certain legumes can immediately access P from unreactive rock phosphates where cereals do not have that ability (Vanlauwe et al., 2000a). Proper targeting of P in legume-cereal rotations has also been shown to significantly enhance the growth of maize after application of rock phosphate to herbaceous legumes (Vanlauwe et al., 2000b). A last alternative to alleviate P stress would be through application of farmyard manure which often contains considerable amounts of available P.Even for N, except for the most efficient N 2 fixing legumes, there is often a need to supply a starter N especially for those legumes growing in low fertility soils. In multiple cropping systems of the tropics, it is possibly only the homestead, the most fertile corner of the farm that may not require external P inputs and/or starter N because of continual application of farmyard manure and household residues.The efficient use of fixed N incorporated in the legume biomass is the net result of the dynamics of N in the system and is affected both by intrinsic characteristics of N sources (legume residues, N fertilizers) and N sinks (crop uptake, soil N pools), and by environmental factors (temperature, soil moisture, rainfall intensity and distribution, etc.) that govern process rates. The decomposition and N release rates of crop residues and green manures depend on their composition (ratio of C:N and content of lignin and polyphenols as well as soil temperature and moisture and the interaction of residues with soil (affected by management) (Palm et al., 2001). N derived from organic sources which is not taken up by the crops or incorporated in the soil organic matter pool may be lost from the system through volatilisation, denitrification, and leaching. Improving synchrony of crop demand with the rate of legume residue decomposition is therefore of fundamental importance for the efficient use of N from leguminous green manures, covers and residues.Within the INM framework, it is now recognized that both organic and mineral inputs are necessary to enhance crop yields without deteriorating the soil resource base. This recognition has a practical dimension because either of the two inputs are hardly ever available in sufficient quantities to the small scale farmer, but it also has an important resource management dimension as there is potential for added benefits created by positive interactions between both inputs when applied in combination. Such interactions can lead to improved use efficiency of the nutrients applied in organic or mineral form or both (Vanlauwe et al., 2001). Two sets of hypotheses can be formulated, based on whether interactions between fertilizer and OM are direct or indirect. For N fertilizer, the Direct Hypothesis may be formulated as: Temporary immobilization of applied fertilizer N may improve the synchrony between the supply of and demand for N and reduce losses to the environment. Obviously, residue quality aspects will strongly determine the validity of this hypothesis. The Indirect Hypothesis may be formulated for a certain plant nutrient X supplied as fertilizer as: Any organic matter-related improvement in soil conditions affecting plant growth (except the nutrient X) may lead to better plant growth and consequently enhanced efficiency of the applied nutrient X.Due to the complexity involved, the efficient use of participatory approaches in the early preadaptive stages of BNF research will ensure that BNF technologies are client-oriented and respond to the needs of farmers and other end-users. Farmer participatory research (FPR) is increasingly receiving considerable recognition in both international and national agricultural research and development organizations as an important strategic research issue, vital to achieving impacts that benefit poor people in marginal, diverse and complex environments. There is now a large body of literature that demonstrates considerable advantages and potentials of involving farmers in the research process. FPR can significantly improve the functional efficiency of formal research (better technologies, more widely adopted, more quickly and wide impacts), empower marginalized people and groups to strengthen their own decision making and research capacity to make effective demands on research and extension services and thus have payoffs both for farmers and for scientists.Legumes very often provide other benefits besides fixed N to the cropping system of which they are part. Although rotational effects of legumes on subsequent cereals have often been translated into N fertilizer replacement values, rotational benefits can not always be explained in terms of N addition to the system. Besides improving the soil physical structure, deep-rooting perennial species may recover nutrients from the subsoil and reverse top-soil degradation (e.g., reverse soil acidification caused by fertilizer use, Vanlauwe et al., 2001). Legumes have also been shown to alter pest and disease spectra and to reduce the Striga seedbank. All the above processes are alleviating a constraint to crop growth and may consequently lead to improved use efficiency of applied N fertilizer, following the indirect hypothesis (section 4.1.5.)Innovations can be considered as demand-driven or as supply-driven. It is fair to say that in the eyes of farmers BNF options may belong to the second category, or at best, are a mixture of both. Furthermore soil fertility decline as an ISFM issue is complex, difficult to prevent given farmers' situation, and easily to detect only when yields drop sharply. This infers that many ISFM innovations will be most effective as conservative or preventative innovations; adopting means often to sacrifice short-term profits for reducing a decline in returns in the future. These innovations have often slow rates of adoption. Simultaneously, farmers vary in their risk preferences of an innovation, and perceptions are affected by information introducing further heterogeneity due different sources of and exposure to information. Often farmers do not face the problem targeted by the innovation or the innovation simply does not work. In addition, farmers will not commit to adoption of an innovation without successfully trialling it. If smallscale trials are not possible or not enlightening for some reason, as frequently the case in heterogeneous and fragile environments that are target regions for BNF, the chances of widespread adoption are greatly diminished. Conducting a trial incurs costs of time, energy, finance and land that could be used productively for other purposes. Furthermore the fact that economic and environmental conditions are rapidly changing today makes the adaptation of present land use systems and the process of including BNF in ISFM largely a process of managing the uncertain.By taking a pro-poor approach, international agricultural research has developed the means to achieve large-scale impacts, responding to the demands of small-scale farmers for improved agricultural production and ecosystem services. Many ISFM options are locally profitable, even under intensely cultivated, land-scarce conditions. The knowledge-intensity and complexity of the ISFM approach, however, makes it difficult to translate local successes from one area to another, unless the factors favouring and constraining adoption are better understood. Increasing our understanding of where ISFM options are working, why, and for whom, will address the constraints limiting their wider use. The cost of not engaging in this research is likely to be enormous, in terms of greater poverty, stagnant and declining production, degraded ecosystem services, and the loss of intellectual property rights related to the local genetic resources of the soil.Facilitating widespread use and impact of ISFM to solve soil fertility problems in the tropics will thus require a tighter linkage and feedback between strategic and adaptive research activities. The iterative process of learning and problem solving builds on indigenous knowledge, improves imperfect technologies, and empowers farmers and institutions. Addressing farmers' problems in a systems context generates management options better suited to their local needs. It also produces policy options that are suited to local institutional realities.The paradigm of involving farmers in research is based on strong evidence (Pretty and Hine 2001) that enhancing farmers technical skills and research capabilities, and involving them as decision-makers in the technology development process results in innovations that are more responsive to their priorities, needs and constraints. It is now widely recognized that these farmer participatory research (FPR) approaches may have wider applications for improving rural livelihoods in complex and diverse low potential areas where a \"systems\" approach is critical for the analysis and improvement of the production systems (Okali et al. 1994).The active involvement of producers in the design of the ISFM system enables researchers and stakeholders to examine and understand the local farming systems and the larger context within which they exist, to incorporate local knowledge into technology innovation, and to develop locally appropriate solutions. A hallmark of FPR approaches is the link it establishes between the formal and local research systems (Ashby et al., 2000). This link enables farmers to express their technology needs and to help shape the technology developed through formal research. Participatory research decentralises control over the research agenda and permits much broader set of stakeholders to become involved in research, thereby addressing the differential needs of men and women for technical innovation.Finally, farmer participatory experimentation and learning approaches represent an investment in the human and social capital available to poor farming families that can be harnessed to provide a systematic feedback process on farmers demands and priorities to research providers. These approaches build farmers' capacity to learn about knowledge intensive processes, biological and ecological complexities (Pretty and Hine, 2001) and can create a sustained, collective capacity for innovation focused on improving livelihoods and the management of natural resources.Scientific and local knowledge can be analyzed in relation to prevailing uncertainties about the innovation using an approach to uncertainty suggested by Rowe (1994). Rowe explains how uncertainty extends through many parts of the decision problem by distinguishing temporal, metrical, structural and translational uncertainty. Temporal uncertainty is associated with fluctuations of processes over time. Metrical uncertainty is introduced by errors associated with the estimation of parameters in a spatially varying resource base. Structural uncertainty is related to the imperfection of the decision model itself. Translational uncertainty arises from contrasts between the perspectives of individuals involved in the decision process.For example: In deciding how to apply fertilizer, metrical uncertainty could be reduced by more precise definition of the relationship between inputs and response. Unlike farmers in highly intensive cropping systems, small-scale farmers in tropical systems do not have ready access to modern monitoring techniques. But they do possess long time series understanding of relations at on one location that has been generated through repeated observations. These accumulated observations can be related to relevant scientific soil parameters presented above, or their local counterparts, providing opportunities for the development of spatially explicit indicators.Temporal uncertainty could be reduced by specifying the phase of crop development for which such a relationship is valid. Farmers have already assembled plenty of experience doing this when deciding, for example, when to enter a fallowed plot into the productive system. Scientists can help to render farmers' experiences made in traditional systems transferable to new cropping circumstances by relating them to underlying processes. On this basis, for example, indicator plants can specifically be selected and grown in new cropping systems. Simple monitoring devices such as leaf colour meters provide more opportunities.Structural uncertainty could be reduced by defining more of the interactions of fertilizer applications with other variables, such as pest and weed infestation or rainfall, and translational uncertainty could be reduced by formulating the actions suggested to reduce the other types of uncertainty in terms which are relevant to the hillside farmers. Reducing structural and translational uncertainty is probably least amenable to formal scientific investigation. Structural uncertainty because of huge complexity of the interactions and the variation in the natural resource base in hillside environments, and translational uncertainty because of the little attention given by scientists to what matters for farmers. To reduce the former, scientists need to understand whether variation matters to farmers, and if so how much of it farmers are willing and able to manage. Relevant and informative trials are essential.If farmers had complete information innovations identified as being relevant would be implemented without delay. Information about complex farming systems and their externalities is however not complete. A pragmatic choice of whether or not to implement an innovation at farm level has to be made about whether or not it is sensible to manage variation more closely, which is based on the interrelated questions of whether as-yet unmanaged variation is significant, whether it is controllable and predictable? All three conditions of significance, control and predictability must be satisfied before improvement can occur.Significance: this is largely a question to be decided by individual farmers. But research has demonstrated that farmers are well aware of problems, and their natural tendency to experiment demonstrates their willingness to change.Control and prediction: in most farms there is uncontrolled variation that is usually of no benefit to farmers. Farmers have the capacity for field-by-field control, and some in-field control. However the capacity to control is limited by farmers' experiences based on long-term observations that usually do relate to traditional cropping systems and control by these means cannot directly be used for new innovations. Second, for control to be effective, the relationship between variation of the controllable inputs and output must also be known to some degree.The key to reducing uncertainty is on-farm trialling, preferably on the farmer's own property. For these reasons, rapid adoption of ISFM management options, involving combinations of unfamiliar and complex innovations that are difficult to trial, are unlikely to occur until they are considered relevant and essential by farmers. Furthermore, even if they are considered relevant and essential, appropriate designs of trialling have to be defined that overcome obstacles including:• Treatments often must be implemented in combinations which make it difficult to determine from field observations alone the individual impacts of each element of the combination. For a trial to be worthwhile, the results of the trial must be observable. • The effectiveness of some innovations may be very sensitive to temporal changes (e.g. weather conditions) or the quality of implementation. As a result trials give highly variable results from time to time.• Economic comparisons based on typical agronomic small-scale research trials can be very misleading. However, the larger the trial is, the less likely the farmer is to make the investment in trialling.Principles of ISFM could influence diverse stakeholders in the tropics to alter the ways they address soils and their management, at a variety of scales. Promotion of ISFM approaches will require increasing participation of national and international research and development organizations, networks, NGO's, and extension agencies working in the tropics. Significant adoption of a range of ISFM technologies has been documented across a number of countries in sub-Saharan Africa. These include (a) integrated nutrient management, (b) micro-dose use of fertilisers, (c) improved manure management practices, (d) inter-cropping systems, (e) integration of multipurpose legumes, (f) improved fallows, and (g) biomass transfer of high quality organic inputs. However, much of these adoption studies have focused on conventional factors influencing adoption of agricultural technologies. The complexity of ISFM technologies and processes require the identification farmers' decision-making processes, constraints and opportunities for the adoption of ISFM technologies, and the identification of farmers' criteria for acceptability of BNF technologies. This will require improving understanding of the complex linkages between livelihood assets and strategies and ISFM adoption, and the impacts of ISFM technologies on rural livelihoods. Measuring the impacts of ISFM is a complex task. We need to develop innovative methods that enable to track changes in the systems through the use of participatory monitoring and evaluation systems to learn from successes and failures.The capacity for ISFM research in the tropics is insufficient both in terms of the numbers of professional personnel and the essential laboratory facilities. ISFM is a knowledge intensive approach to soil management. Professional staff and students alike suffer from isolation and lack of access to up-todate educational opportunities. Networks run by SROs and CGIAR Centres, such as the TSBF African Network for Soil Biology and Fertility (AfNet) and MIS (Integrated Management of Soils) consortium in Central America provide a vehicle of opportunity to correct this situation. A substantial number of short term, degree-related, and on-the-job training activities, across the tropics could help spread ISFM approaches at all national levels, including university curricula. Some of the groundwork for scaling up and out has been laid through an emphasis on the synthesis of results and dissemination of information on the technologies and on developing partnerships between research, extension services and NGOs. TSBF-CIAT researchers have experience in developing and applying decision guides to assist extension staff and farmers in selecting among soil fertility options for different situations (Palm et al., 2001). The use of accessible, user-friendly GIS tools and geo-spatial datasets for the region can be used in the scaling process, by identifying recommendation areas for BNF technologies. Scaling up requires sustained capacity building to build the requisite skills among the NARS to ensure that the work is involving and reaching the intended beneficiaries. It also requires building local capacities and empowering rural communities to improve their technical skills and decision-making on soil fertility, in support of scaling up and sustaining impacts of ISFM technologies. Efforts to engage with policy makers and private sector input suppliers and dealers should also be strengthened.In this brief position paper we have argued that BNF is a key input to ISFM strategy to combat soil fertility degradation and for sustainable intensified agriculture in the tropics. The reasons for lack of success in solving the soil fertility problem lie substantially in the failure to deal with the issue in a sufficiently holistic way. Soil fertility decline is not a simple problem. In ecological parlance it is a 'slow variable', which interacts pervasively over time with a wide range of other biological and socio-economic constraints to sustainable agroecosystem management. It is not just a problem of nutrient deficiency but also of inappropriate germplasm and cropping system design, of interactions with pests and diseases, of the linkage between poverty and land degradation, of often perverse national and global policies with respect to incentives, and of institutional failures. Tackling soil fertility issues thus requires a long-term perspective and holistic multidisciplinary systems approach of integrated soil fertility management.Developing adoptable legume-BNF technologies to combat soil fertility degradation remains to be a major challenge. Research and development efforts are needed to integrate BNF efficient and stress adapted grain and multipurpose legume germplasm into production systems to intensify food and feed systems of the tropics. Several key interventions are needed to achieve greater impact of legume-BNF technologies to improve livelihoods of rural poor. These include (a) integration of stress-adapted and BNF efficient legume cultivars in rotational and mixed cropping systems, (b) strategic application of inorganic fertilizers and organic residues to facilitate efficient nutrient cycling and appropriate replenishment of soil organic matter, (c) adoptable strategies of soil and water conservation, (d) integrated pest/disease/weed management through the use of biotic stress resistant germplasm with minimum pesticide/herbicide applications, (e) marketing strategies that are economically efficient, and (f) development of an appropriate policy and institutional environment that provides incentives to farmers to adopt legume-based BNF technologies.Local knowledge related to agriculture can be defined as the indigenous skills, knowledge and technology accumulated by local people derived from their direct interaction with the environment (Altieri 1990). It is the result of an intuitive integration of local agroecosystem responses to climate and land use change through time (Barrios et al. 1994). Transfer of information from generation to generation undergoes successive refinement leading to a system of understanding of natural resources and relevant ecological processes (Pawluk et al. 1992). WinklerPrins (1999) has provided a recent review of the scope and nature of the existing literature about local soil knowledge and the emerging science of ethnopedology.There is increasing consensus about the need for enhanced understanding of local knowledge in planning and implementing development activities (CIRAN 1993). The slow rate of assimilation of new technology and new cropping systems has been often attributed to local inertia rather than the failures to take into account the local experience and needs (Warren 1991). According to Walker et al. (1995), increased application of indigenous knowledge to rural research and development can be attributed to the need to improve the targeting of research to address client needs and thus increase adoption of technological recommendations derived from research. Besides, ethical considerations related to participation and empowerment of local communities have gained considerable importance (Chambers 1983).The complementary role that indigenous knowledge plays to scientific knowledge in agriculture has been increasingly acknowledged (Sandor and Furbee 1996). Experimental research is an important way to improve the information upon which farmers make decisions. It is questionable, however, if relying on experimental scientific methodology alone is the most efficient way to fill gaps in current understanding about the sustainable management of agroecosystems. There has been limited success of imported concepts and scientific interpretation of tropical soils in bringing desired changes in tropical agriculture. This has led an increasing recognition that the knowledge of people who have been interacting with their soils for long time can offer many insights about managing tropical soils in a sustainable way (Hecht 1990).Nevertheless, although benefits of local knowledge include high local relevance and potential sensitivity to complex environmental interactions, without scientific input local definitions can sometimes be inaccurate and unable to cope with environmental change. It is thus argued that a joint local/scientific approach, capitalizing on complementarities and synergies, would permit overcoming the limitations of site specificity and empirical nature and allow knowledge extrapolation through space and time as suggested by Cook et al. (1998).The science of ethnopedology encompasses many aspects, including indigenous perceptions and explanations of soil properties and soil processes, soil classifications, soil management, and knowledge of soil-plant inter-relationships (Talawar 1996). This paper examines three case studies on local soil knowledge and management and the implications of these results on future research on integrated soil management in Latin America. Results from case studies to elicit local information using key-informants are reported for small farmers from Orinoco floodplains in Venezuela and from the Cabuyal river watershed in Cauca, Colombia. A participatory approach was used with farmers from the Tascalapa river watershed in Yoro/Sulaco, Honduras, in order to identify and classify local indicators of soil quality related to permanent and modifiable soil properties. Finally, the potential of the latter approach as a mechanism to facilitate collective action leading to integrated soil management is discussed.The local knowledge about soils and their management by Orinoco floodplain farmers was studied by Barrios et al. (1994). A case study approach with key-informants was used to highlight practices that lead floodplain farmers to high yields and economic success while improving or maintaining soil fertility (Anderson andIngram, 1989, Brown et al., 1994). In this highly unpredictable environment, the basic assumption is that farmer's indigenous knowledge is the result of an intuitive integration of their perception of changes in the agroecosystem as a result of climatic changes, the major driving force for decision making. The systematic assessment of local knowledge about soils and their management focused on criteria used for selection of new agricultural sites in this typically slash and burn agriculture, for soil classification and soil texture \"management\" and for managing inherent soil variability.In the Orinoco floodplains, when farmers are looking for new cropping land they make a first selection based on the type of vegetation growing on the soil. Therefore, traditional farmers use associations of native plants as indicators of soil quality. In order of importance, trees such as 'caujaro' (Cordia sp.), 'taparo' (Crescentia sp.) and 'yagrumo' (Cecropia sp.) and herbaceous species like 'gamelote' (Paspalum fasciculatum), 'paja de agua'(Paspalum repens), 'tarraya'(Glinus sp.) and 'borrajón' (Heliotropium indicum) were used as indicators of \"good soils\" (Table 1). Conversely, they also use native plants as indicators of where not to establish a cropping field. For instance, trees such as 'melero' (Combretum frangulaefolium) and 'toco' (Crataeva gynandra) as well as herbaceous species like 'yerbabuena' (Phyla betulaefolia) and the grasses 'pata colorada' and 'bochocha' were plants indicating \"bad soils\". It is not surprising that farmers use vegetation in their first evaluation of potential cropping sites since these integrate complex and often diffuse soil attributes.Once the agricultural plot has been selected a more detailed examination of the soil allows farmers to plan crop and soil management activities. While darker colored soils are generally recognized as better soils, local farmers identified soil texture as the most important measure on which to select crop and soil management practices. Farmers recognized the importance of fine texture sediment in floodplain soil fertility. Given the great uncertainty of sediment quality every year as influenced by flooding regimes, a traditional system to manage the quality of the incoming sediments was developed by floodplain farmers (Barrios et al., 1994). Vegetation barriers are allowed to grow or are planted by farmers around their agricultural plots in order to \"filter\" the coarse sediment and only allowing the finer sediment into the plots. Vegetation barriers are typically composed of trees like 'Jariso'(Ruprectia sp.), 'guayabo rebalsero' (Psidium ovatifolium) and grasses like 'gamelote'(P. fasciculatum) (Fig. 1). Soil heterogeneity is very conspicuous because of the uneven distribution of sediment throughout the floodplain. The use of different crops in areas with different soil texture by traditional farmers shows an optimization of soil resource use. This could be seen as a traditional basis for modern site-specific management. Local wisdom indicates that while certain crops only grow well in specific soil textures, e.g., watermelon in sandy soil, beans in clay soil and cotton in mixed soil, other crops such as maize and cowpea are ubiquitous and are found in all soil textures (Barrios 1997).Figure 1. Schematic diagram of vegetation barriers used by Orinoco floodplain farmers to manage the quality (particle size) of the incoming sediment into their agricultural plots (modified from Barrios et al., 1994).Studies on local knowledge about soils and their management were conducted within the Cabuyal watershed, Cauca department -Colombia using case study approaches with semi-structured questionnaires, participatory farm mappings of soil qualities and identification of local indicators used to discriminate among different soils (Trejo et al. 1999). Previous studies in the area by CIAT (Centro Internacional de Agricultura Tropical) during the last 15 years facilitated the identification of key informants from each village. Key informants were selected from eight villages in three altitudinal zones in the watershed (Salamanca 2000). High elevation villages (1700-2200 m.a.s.l.) included: El Cidral, La Esperanza, La Primavera and El Rosario, middle elevation villages (1450-1700 m.a.s.l.) La Campiña and El Porvenir, and low elevation villages (1175-1450 m.a.s.l.) included La Llanada and La Isla. In the predominantly young volcanic-ash soils, Oxic Dystropepts in the USDA soil classification system, 100% of farmers interviewed use soil color for classification and assessment of soil quality. Black colored soils are considered good for cropping and yellow and red soils are considered marginal. Black soils are often found in soils under forest, fallow or pastures. Increasing use of tillage has lead to increased rates of soil loss and thus the usually darker topsoil has given way to the red sub-soil where cultivation is now taking place in many agricultural plots.Native plants constitute another means by which Andean hillside farmers classify the soils in their farms (Barrios and Escobar, 1998). In Table 2 we find native plants used as indicators of soil quality by farmers in the Cabuyal river watershed. Fertile soils are characterized by trees like 'nacedero' (Trichanthera gigantea) and 'guamo' (Inga sp.) and herbaceous plants like 'papunga' (Bidens pilosa) and 'mariposo'(Clibadium surinamensis) while plants predominating in poor soils invariably include 'helecho marranero' (Pteridium arachnoideum) and 'paja garrapatera' (Andropogon bicornis).Farmers also identify ubiquitous species such as 'yaraguá' (Mellinis minutiflora) and 'caracola' (Koheleria lanata) which are then characterized by their vigor and leaf color. Darker green colored leaves are associated with more fertile soils while yellowish colors are indicative of poor soils. Soils are also classified by their structure into 'polvoso' or \"powdery\", that is, with no macroaggregates indicating degraded soils on the one hand, and 'granoso' or \"grain-like\" which indicates some level of aggregation associated with better soils. This is an important characteristic used by farmers to assess soil recuperation after degraded soils have been left uncultivated to \"rest\" or fallow. In these hillside soils, topographic position also plays an important role in local soil classification. Hill tops or 'cimas'are identified as containing poorer soils, while the quality of hillsides or 'lomas' depends on how steep is the slope is. The more fertile soils are concentrated in the flat areas or 'planadas', hollowed areas or 'huecadas' because of the accumulation of eroded soils lost from up the hill as well as riverine floodplains by deposition of nutrient rich sediments (Cerón 2000). Inherently infertile soils are named 'tierra brava' or \"angry soils\" which should be distinguished from 'tierra cansada' or \"tired soils\" which are soils degraded by inappropriate management. Farmers consider that while the former are likely to respond to fertilizer applications (i.e. chicken manure) the latter invariably needs a period of fallow phase to recover lost attributes.A participatory approach was used in Honduras to identify and classify local indicators of soil quality and details can be found in Trejo et al. (1999). In short, six communities were selected from the Tascalapa watershed, namely Santa Cruz, Mina Honda (higher zone), San Antonio, Jalapa and Luquigue (middle zone) and Pueblito (lower zone) to identify and classify local indicators of soil quality at a landscape scale. Brainstorming sessions with farmer groups from the six communities respectively were followed by a prioritization phase where farmers from each community were split in smaller groups in order to rank local soil quality indicators identified according to their relative importance using paper cards. The final list of local indicators, in order of importance, was then integrated with their corresponding technical indicator in plenary sessions and organized into indicators of permanent (Tables 3) and modifiable (Table 4) soil properties.Although some local indicators can be rather general like fertility, slope, productivity and age under fallow, other local indicators are more specific. For instance, plant species growing in fallows, soil depth, color, water holding capacity and predominant soil particle sizes provide indicators that can be easily integrated with technical indicators of soil quality.The classification of local indicators into permanent and modifiable factors provides a useful division that helps to focus on those where improved management could have the greatest impact. This strategy is particularly sound when there is considerable need to produce tangible results in a relatively short time in order to maintain farmer interest as well as to develop the credibility and trust needed for wider adoption of improved soil management practices.Key permanent soil properties captured by local indicators that are commonly perceived as important by farming communities included slope, soil depth, soil color, soil texture and soil structure. The importance of slope in this hillside environment is obvious as there is a maximum inclination under which agriculture can be practiced. Because of their topography, hillside soils are prone to erosive processes even under natural vegetation or appropriate management. These soils tend to be relatively shallow compared to valley soils and therefore local farmers identify a minimum soil depth required for crop root growth and development (i.e. 12 inches, half a cutlass). Soil color provides a good measure of inherent soil fertility where black soils are seen as good soils and other red and yellowish colors as bad soils. Nevertheless, despite being classified as a permanent property, local farmers recognize that management practices involving crop residue additions could darken light colored soils indicating improvement in their quality. Soil texture is considered important by local farmers because it affects soil water holding capacity as well as the resistance to tillage. Soil workability is also related to soil structure, as good soils are perceived as those that do not compact, and where soil aggregates can be broken by tillage.Modifiable soil properties of importance were perceived as those related to the lack or presence of burning, the type of native vegetation and the soil biological activity indicated by the presence of soil organisms (i.e. earthworms). The earliest farmers have used fire as an agricultural management tool to recover nutrients held in the native vegetation biomass for the crops, to control pests and to dispose of perceived \"excess\" plant biomass in the fields (Sanchez, 1976). Despite the realization of the harm done by annual fires on the soil, the lack of farmer consensus that could lead to a concerted action appears to be an important limitation. The participatory methodologies presented here have the potential to facilitate consensus amongst the local farmer community on high priority problems and opportunities. In this capacity, their linkage to concrete plans of action, as explained by Thomas et al. (2000), suggests this approach as a way to promote collective action at a landscape scale. A similar rationale has been successfully used in Africa to stimulate the participatory learning and action research process by Defoer and Budelman (2000).It is important to note that the type of native vegetation present in a soil is a local indicator of soil quality (Table 5) that not only cuts across the communities studied in Honduras but also across the other two case studies reported in this article. This observation suggests that there may be an underlying fundamental ecological principle behind farmer observations in the three locations. It is proposed here that one such ecological principle is that of natural succession as suggested by Paniagua et al. (1999). Natural and agricultural ecosystems respond similarly to degradation or regenerative processes through natural succession.Table 3. Integration of local and technical indicators of soil quality related to permanent soil properties identified and ranked according to their importance by Honduran hillside farmers from different villages (adapted from Turcios et al., 1998 The most adapted plants and organisms in the soil gradually replace less adapted ones as continued selective pressures are exerted (i.e. during regeneration of soil fertility or soil degradation). Native plants and \"weeds\", as biological indicators, have the potential to capture subtle changes in soil quality because of their integrative nature. They reflect simultaneous changes in physical, chemical and biological characteristics of the soil. There is considerable scope, therefore, to further explore the use of local knowledge about native plants as indicators of soil quality and as a tool guiding soil management decisions. Farmers are often more enthusiastic to empirical approaches (i.e. local knowledge, on-farm experiments) than prescriptive approaches (i.e. scientific knowledge, recipes for soil management) (Cook et al., 1998). Figure 2 illustrates that while scientific information can be very precise its relevance can be relatively low. On the other hand, while local information can be relatively imprecise, yet, it can be very relevant. Although information should ideally be certain in both meaning and context, in reality this is not the case. Research efforts should further explore a suitable balance between precision and relevance as seen in the figure . The methodological approach proposed by Trejo et al. (1999) goes beyond the identification and classification of local indicators of soil quality. It rests on the hypothesis that in order for sustainable management of the soil resource to take place, it has to be a result of improved capacities of the local communities to better understand agroecosystem functioning. Improved capacities by technical officers (extension agents, NGO's, researchers) to understand the importance of local knowledge is also part of the methodology. Therefore, after identifying if there is poor or a lack of adequate communication between the technical officers and the local farm community as a major constraint to capacity building, the methodology proposed deals with ways of jointly generating a common knowledge that is well understood by both interest groups. The structure of the guide is shown in Fig. 3 shows the different sections of the methodological guide.Section 1, which provides a general overview of soil formation factors and processes, based on Jenny's seminal work (Jenny, 1941(Jenny, , 1980)), is presented in order to bring the trainees (e.g. technical officers) to a common starting point. Section 2 deals with participatory techniques that help gather, organize and classify local indicators of soil quality through consensus building. Section 3 attempts to find correspondence between local indicators and technical indicators. This is carried out in a plenary session exercise of integration where the most important local indicators of soil quality are analyzed in the context of technical knowledge and are classified into indicators of permanent or modifiable soil properties. The idea is to provide a guideline to focus efforts on soil properties where management can have an impact. An important part of this section is the Soils Fair for farmers that is organized by the trainees. The Fair aims to help farmers develop skills to characterize relevant physical, chemical and biological properties of their soils through simple methods that can then be related to their local knowledge about soil management.The result of this two-way exchange process has a positive impact on the technical knowledge by nurturing it with local perceptions and demands. The number of successful experiences in natural resource management in agroecosystems will likely increase because of the solid basis provided by local relevance. On the other hand, local knowledge will also be enriched because of greater possibilities for its wider comprehension, appreciation and use. Local communities will be empowered by the joint ownership of the technical-local soil knowledge base constructed during this process.The two-way improvement of communication channels will likely improve the communication of farmer's perceptions to extension agents and researchers as well as make recommendations by extension agents and NGOs better understood by the farmer community. Better communication opens opportunities for established and/or emerging local organizations to use the methodological approach for consensus building that precedes any collective actions for improved natural resource management through integrated soil management. Trejo et al. 1999). The considerable importance of local knowledge in guiding future research and development efforts towards a sustainable management of natural resources is highlighted in this study. The case studies presented showed that there is a consistent rational basis to the use of local indicators of soil quality. The use of key-informants was an effective method to elicit local information about soils and their management. In addition, participatory approaches involving group dynamics and consensus building are likely to be key to improve soil management beyond the farm-plot scale to the landscape scale through the required collective action process.Native plants as local indicators of soil quality were important local indicators of soil quality in all three case studies associated with modifiable soil properties. The use of indicator plants, belonging to the local knowledge base, when related to management actions could ease adoption of improved technologies. This approach would allow the use of plants as indicators of soil quality to which local farmers can relate more closely than to common agronomic measures such as phosphorus availability, organic matter content or pH value. Additional research could also include further integration of scientific spatial analysis (i.e. GIS, topographic modeling) with the spatial perception of natural resources by farmers aiming at improved implementation of site-specific management.Hillsides of tropical America cover about 96 million hectares (Jones, 1993) and have important roles as reserves of biodiversity and source of water for areas downslope (Whitmore, 1997). A high proportion of the Colombian Andean soils (i.e. 83% ) suffer from erosion problems (Amezquita et al., 1998). These soils, particularly the volcanic-ash soils, usually contain high levels of soil organic matter (SOM) but low availability of nutrients due to SOM protection by mineral particles which limits decomposition (Phiri et al., 2001). According to Shoji et al. (1993) plant growth in volcanic-ash soils is limited by the low availability of N and P together with low base saturation and deficiency of some micronutrients (Cu, Zn and Co).Use of green manures could reduce soil exposure to erosive processes, promote a greater nutrient cycling and improve the synchrony of nutrient release with crop demand. However, the potential benefit of green manures as a source of nutrients to crops can only be achieved if their decomposition and nutrient release patterns are known so that the synchrony of nutrient release with crop nutrient demand can be improved (Myers et al., 1994). Management options include the selection of plant materials with different chemical composition (quality) and by controlling the timing, quantity and form of application to the soil (Anderson and Ingram, 1993;Palm, 1995;Palm et al., 2001). Besides, the single or combined applications of plant parts used as green manures (i.e. leaves, stems) are likely to influence the decomposition and nutrient release rates to the soil (Lehmann et al., 1995;Handayanto et al., 1997).Several methods have been used to determine decomposition and nutrient release of plant materials in the field, and the litterbag technique is probably the most widely used because of its simplicity, replicability, and ability to selectively exclude classes of soil fauna (Vanlauwe et al., 1997a). However, while this method may underestimate dry matter and nutrient losses it is considered a great tool for treatment comparisons (Vanlauwe et al., 1997a and b). For this technique, standard quantities of litter are enclosed in nylon-mesh bags; litterbags are then incubated in the field, and weight and nutrient loss are monitored during several weeks by partial retrieval of litterbags.The quality of plant materials has been considered one of the most important factors that affect decomposition and nutrient release (Heal et al., 1997;Swift et al., 1979). High nutrient contents in plant materials have generally been correlated with high decomposition rates (Gupta and Singh, 1981). Other researchers have found that low lignin/N ratio (L/N) also leads to faster decomposition (Melillo et al., 1982). According to Palm and Sanchez (1990), polyphenol (PP) concentrations can influence decomposition and nutrient release rates in legume materials to a greater extent than lignin (L) or N content. Furthermore, Thomas and Asakawa (1993) reported that the C/N, L/N, PP/N and (L+PP)/N ratios were all inversely correlated with N release rates from herbaceous materials; while weight loss only correlated with the L/N and (L+PP)/N ratios. More recent studies also showed similar correlations between the (L+PP)/N ratio and decomposition and N release for several agroforestry species (Barrios et al., 1997;Lehmann et al., 1995;Mafongoya et al., 1998;Vanlauwe et al., 1997b). Tian et al. (1996), on the other hand, showed that decomposability of plant residues placing nylon-mesh bags inside the rumen of fistulated animals significantly correlated with that using litterbags in the field. Using a similar principle, another promising plant quality index is the in vitro dry matter digestibility (IVDMD) lab test used for animal feed (Harris, 1970). Although the decomposition processes in the rumen and the soil differ, they are sufficiently similar to be thought of as a potential method for comparative plant tissue studies (Chesson, 1997).In this study we determined the decomposition and release of N, P, K, Ca and Mg by 12 plant materials used by farmers in our study areas. These plant materials were surface applied to a soil in a tropical hillside agroecosystem, and we assessed the relationship of some common plant quality indices and IVDMD for such materials to their respective decomposition and nutrient release rates.The study was carried out at 'San Isidro' experimental farm located in Pescador, Cauca department, Colombia, at 2° 48' N, 76° 33' W and 1.500 masl. The area has a mean temperature of 19.3 o C and a mean annual rainfall of 1900 mm (bimodal). The experiment was conducted from April to August during the first cropping season of 1998 (Figure 1).The experimental plot had a slope of approximately 30%. The soils, derived from volcanic ashes, have been classified as Oxic Dystropepts (Inceptisols) in the USDA soil classification system (USDA, 1998). Soil characteristics include: pH (H 2 0): 5.1,50 9 kg -l C, 3 9 kg -l N, 4.6 mg kg -l soil of Bray-II P, and 1.1, 0.6, 2.5 and 0.9 cmol kg -1 soil of Al, K, Ca and Mg, respectively. Soil bulk density was 0.8 g cm -3 and allophane content ranged from 52 to 70 g kg -l (Phiri et al., 2001). Plant materials were selected from plants with known adaptation to the hillside environment and also with contrasting quality. Since two species and three varieties of Mucuna are utilized by farmers in our study areas and they show differences in agronomic behaviour it was considered important to evaluate their decomposition and nutrient release rates. Plant materials included: leaves, with petioles, of IITA-Benin (MPITm) and Indigofera constricta (INDm), in the proportion found at the time of pruning and collection. Stems and the mixture of leaves and stems were studied to relate to common farmer practice of mixed application. It also contributed to expand the quality spectrum of materials evaluated and to compare them with the leaves alone. Pruned materials were collected from herbaceous plants (Canavalia and 'Mucunas') and Tithonia at flowering, while materials from trees (Cratylia and Indigofera) were pruned 6 months after the last pruning. Harvest time for herbaceous materials followed farmer practices and for tree materials was associated with optimal pruning regime identified in previous unpublished studies.After collection, each plant material was air and oven dried (60 °C), thoroughly mixed and composited, and a sample was taken for chemical analyses. Then, 15 g of each plant material were placed inside litterbags (20x20 cm nylon bags, mesh size 1.5 mm), corresponding to an application rate of 3.75 Mg dry matter ha -l . Litterbags were placed on the soil surface between maize rows in a randomized complete block design with four replications. The maize crop did not receive any additional treatments besides residue quality. At 2, 4, 8, 12 and 20 weeks, one litterbag of each repetition and treatment was collected, manually cleaned, and washed with distilled water to remove soil particles. Remaining plant material was air and oven dried (60 °C) to constant weight before determining dry weight and nutrient contents. Subsamples of plant materials used in litter bags were analyzed for their in vitro dry matter digestibility (IVDMD), total carbon (C), nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), and contents of acid detergent fibre (ADF), neutral detergent fibre (NDF), hemicellulose (HEM), lignin (L), polyphenols (PP) and N fixed to ADF (N-ADF). In addition, the amount of plant material retrieved from litterbags at each sampling time was analyzed for total N, P, K, Ca, Mg and ash content.All plant material was ground and passed through a 1-mm mesh before analysis. C, N and P were determined colorimetrically with an autoanalyzer (Skalar Sun Plus, Breda, The Netherlands), and K, Ca and Mg with an atomic absorption spectrophotometer (Unicam 969, Reading, U.K.). ADF, NDF and lignin were determined using modified techniques of Van Soest and Vine (Harris, 1970) and total polyphenols with a modified Anderson and Ingram (1993) method that uses 70% methanol, 0.5% formic acid and 0.05% ascorbic acid as extractant (Telek, 1989), the Folin-Ciocalteu reagent and tannic acid as standard. HEM was calculated by substracting ADF from NDF. IVDMD was determined by the modified methodology of Tilley and Terry, that includes a 48-h incubation of plant materials with rumen microorganisms followed by acid/pepsine digestion (Harris, 1970). Ash contents were determined by heating at 550 °C for 2 h and these data were used to correct the weight of the plant material remaining for contamination with soil.Decomposition of plant materials and their N, P, K, Ca and Mg release were evaluated through assessment of dry weight and nutrient losses from the materials. The percent of dry weight remaining (DWR), and nutrients remaining (NR, PR, KR, CaR and MgR), for each experimental unit, was calculated as shown:where XR is the percent weight or nutrient remaining, Xt the weight or nutrient content at each sampling time and Xo the starting weight or nutrient values. Dry weight and nutrients remaining were subjected to analysis of variance (ANOVA) at each sampling time. Standard errors of the difference in means (SED ) were calculated from the ANOVA and reported with the data. Whenever necessary, variables were logtransformed to normalize data.In order to describe treatment trends, treatment means of dry weight and nutrients remaining were regressed over time using a single exponential decay model (Wieder and Lang, 1982). This model is described by the following equation:where XRt is the dry weight or nutrient remaining at time t and the slope k, the decomposition or nutrient release constant. This model has been recently used by Palm et al. (2001) in their Organic Resource Database (ORD) to allow comparisons of the derived rate constants, over similar evaluation times, for different species and experiments. Root square errors were used to assess fit of the model used.Correlation and linear regression analyses were carried out between chemical parameters of the plant materials used in litterbags and their decomposition and nutrient release rates. The amount of nutrients released by plant materials to the soil was calculated by substracting the nutrients remaining in the residues at the end of the field incubation from the total amount of nutrients initially applied. SAS (SAS Institute, 1989) was used for the statistical analysis. TTH showed the lowest C, NDF, HEM and lignin contents, and the highest IVDMD, K, Ca and Mg values. Conversely, INDs and MPITs had the lowest N and N-ADF contents, but the highest C/N, PP/N and (L+PP)/N ratios (Table I). These materials, along with CRA, were also characterized by having high ADF and NDF, low IVDMD and high L/N ratio. In addition, CRA had the highest lignin content and N/P ratio, but lowest PP, IVDMD and PP/N ratio, while MDEE had the highest N, P, HEM and PP contents, and the lowest ADF value and C/P ratio (Table 1).Large dry weight losses occurred in the first 2 weeks of the experiment but subsequently slowed down and remained relatively stable after week 12 (Figure 2a). A similar pattern was observed for nutrient release (Figures 2b-f), with the exception of Ca release in three of the treatments studied (Figure 2e). Significant differences (p<0.05) were found among treatments as shown by SED bars in Figure 2.Using the single exponential model to fit data was possible to find that the best fits were found for K release, while the worst fit was found for decomposition, as shown by the lowest and highest root square errors, respectively (Table 2) d -l ) and Mg (k Mg , d -l ) release rates and root square errors (Syx) obtained when fitting the treatment mean values of dry weight and nutrient remaining against time using the single exponential model (Wieder and Lang, 1982) Relationships between the quality of plant materials and their decomposition and nutrient release rates A significant positive correlation (P<0.05) was found between decomposition rates and N, K, Ca and Mg content, and IVDMD; while a negative correlation was found for ADF, NDF, lignin and the C/N, C/P, L/N and (L+PP)/N ratios (Table 3). The quality parameters showing the stronger relationships were NDF (r = -0.959, P<0.001) and IVDMD (r = 0.871, P<0.001). These relationships could be represented by linear regressions between these quality parameters and decomposition rates (Figure 3), where k D =0.057-0.0008*NDF (R 2 =0.92) or k D =-0.0381 +0.0009*IVDMD (R 2 =0.76). Some quality parameters of plant materials were also significantly correlated to nutrient release rates (Table 3). While N content and IVDMD showed a positive correlation with N release rates, ADF, NDF and lignin contents and the C/N, L/N and (L+PP)/N ratios were negatively correlated. The best indicators of this process were the L/N and (L+PP)/N ratios and IVDMD as indicated by their greater correlation coefficients. For P release, significant correlations were found for Ca and N-ADF contents and the C/N, N/P and PP/N ratios. While K release rates were only correlated with C and HEM contents, Ca release rates correlated with C, K, Ca, N-ADF and HEM contents and the N/P ratio. Mg release rates only correlated with C/P ratios Total release of nutrients, from plant materials after 20 weeks (Table 4), showed that higher amounts of N were released by MDEE, MPBR, IND and TTH (124-144 kg ha -l ), while MPITs and INDs showed the lowest N release (27.6 and 41.5 kg ha -l , respectively). The largest amount of P was released by MDEE and MPBR (11.4 and 8.9 kg ha -l , respectively), and the lowest by CRA and INDs (3.5 kg ha -l ). TTH, on the other hand, presented the highest release of K, Ca and Mg amounts among all treatments evaluated (129.3, 112.6 and 25.9 kg ha -l , respectively); while the lowest release of these nutrients was 48.3 kg ha -1 K in INDs, 4.4 kg ha -l Ca in MPITs and 10.6 kg ha -l Mg in CAN. Decomposition and nutrient release of plant materials generally followed an exponential trend. Differences among plant materials were related to tissue quality even among closely related species (i.e. Mucuna). Significant relationships were detected between the quality of plant materials and their respective decomposition and nutrient release rates (Table 3). NDF and IVDMD were the quality parameters most related to decomposition rates in this study (Table 3; Figure 3). Our results for NDF are consistent with results by Gupta and Singh (1981) showing that plant cell wall content is an important predictor of decomposition rates. On the other hand, although Tian et al. (1996) estimated the decomposability of plant residues by an 'in vivo' ruminant nylon-mesh bag assay, the use of IVDMD as an index related to decomposition in the field has not been reported elsewhere. The highly significant (P<0.00l) correlations obtained in this study between NDF and IVDMD, and plant decomposition suggests that lab-based NDF or IVDMD tests could be used as surrogates for decomposition of plant tissue. This finding could be of practical importance for screening of plant materials for different farm uses. Such tests can save time and reduce variability associated with decomposition studies in the field. Other indices studied which have already shown potential in the literature include N and lignin content, and the C/N, L/N and (L+PP)/N ratios because of their correlation with decomposition rates (see Mafongoya et al., 1998).The rates of nutrient release were also related to the chemical composition of the plant materials studied as shown in Table 3. Higher correlations were found for N release and the L/N and (L+PP)/N ratios, for P release and the C/N and N/P ratios, for K release and the C and HEM contents, for Ca release and Ca and HEM content, and for Mg release and the CIP ratio. Previous studies have shown that lower N release rates from plant materials were related to high L/N ratios (Singh et al., 1999;Thomas and Asakawa, 1993) and (L+PP)/N (Barrios et al., 1997;Handayanto et al., 1994;Lehmann et al., 1995;Thomas and Asakawa, 1993;Vanlauwe et al., 1997b) for several species. P mineralization rates from decomposing plant materials have been correlated to N/P ratios (Palm and Sanchez, 1990) and L/N and C/N ratios (Singh et al., 1999) and this may be related to different decomposer communities developing on plant materials of different quality. Ca release, on the other hand, has been related to cell wall constituents (Attiwill, 1976;Luna-Orea et al., 1996) and polyphenols (Lehmann et al., 1995). Mg release, also, has been related to cell wall constituents (Luna-Orea et al., 1996) and to initial Mg content in the tissues (Lehmann et al., 1995). Nevertheless, the high potential for K leaching from plant tissues is probably responsible for the limited reports in the literature of significant relationships between plant quality indices and K release as suggested by Tian et al. (1992).High initial nutrient contents in plant materials can be responsible for high decomposition and net nutrient release because of enhanced microbial growth and activity; however, considerable contents of structural polysaccharides like HEM and lignin, can reduce the effect of initial nutrient content because of physical protection of other cell constituents from microbial attack (Chesson, 1997;Mafongoya et al., 1998). Polyphenols in plant tissues can also reduce decomposition and nutrient release by binding of cell wall constituents and proteins (i.e. Vanlauwe et al., 1997b). The type of polyphenols and their relative content in plant tissues is also important to consider when studying N mineralization from plant materials because different polyphenols have different chemical activities. Earlier studies indicate that the method of drying of legume plant materials has an effect on type and concentration of polyphenols. It has been shown that ovendrying can reduce soluble polyphenol concentrations compared to airdrying (Mafongoya et al., 1997).Our total polyphenol values are generally higher than those reported for other tropical plant materials as reviewed by Mafongoya et al. (1998). Higher values may be a result of methodological differences. While higher tissue: solvent ratios may not extract all polyphenols (Constantinides and Fownes, 1994) our modified extraction method using a greater proportion of methanol (70% ) compared to the Anderson and Ingram (1993) standard methodology (50%), as well as the addition of formic and ascorbic acids as antioxidants may have led to higher total polyphenol values. In addition, plants growing in soils with poor N availability, as in the case of volcanic-ash soils, can result in higher concentrations of polyphenols than those growing in more fertile soils (Palm et al., 2001).Decomposition and nutrient release trends for each treatment (Table 2; Figure 2) suggest that these processes are related. Significant correlations (P<0.05) were found between decomposition and N release (r = 0.596), K and Ca release (r = 0.912) and K and Mg release (r = 0.636) (data not shown). Significant correlations were also found by Singh et al. (1999) between dry weight loss and N release rates. Lehmann et al. (1995), on the other hand, found significant correlations among dry matter, N and Ca losses, but no connection between Mg or K with N and dry matter losses.Although decomposition and nutrient release rates found in this study were sometimes higher than those reported by other researchers using similar methodology they fall within the range observed for tropical zones (Handayanto et al., 1994;Mafongoya et al., 1998;Mwiinga et al., 1994;Palm and Sanchez, 1990;Thomas and Asakawa, 1993;Tian et al., 1992). The high rates found in this study could be linked to the intrinsic characteristics of the materials used (species, age, quality, etc.) but also to the climatic conditions (i.e. high moisture and favourable temperature) during the first weeks of the experiment (Figure 1). It is well known that temperature and precipitation can influence the pattern and rate of decomposition of plant materials (Gupta and Singh, 1981).Nitrogen release rates in this study were higher than those of dry weight loss (Table 2), and this is consistent with previous studies by Mwiinga et al. (1994), Palm and Sanchez (1990), Schroth et al. (1992) and Tian et al. (1992). Phosphorus release rates were usually higher than decomposition rates with the exception of TTH and IND. This could be interpreted as potential for a more gradual P release to the soil from these plant materials. Although K and Mg release rates were higher than decomposition rates, Ca release rates were lower for CRA, MDEE and MPITs as they presented initial immobilisation. Ca immobilisation has been previosuly reported by other authors (Lehmann et al., 1995;Palm and Sanchez, 1990;Schroth et al., 1992) and generally explained by the accumulation of Ca by funghi on decomposing residues.Plant parts of the same species often show different patterns and rates of decomposition and nutrient release. In our study, plant leaves showed faster decomposition and N release rates than mixtures of leaves and stems, and these mixtures were faster than stems evaluated. In contrast, P release showed the opposite trend (Table 2). For K, Ca and Mg release, however, no consistent trends were found. These findings suggest that potential nutrient contributions by green manures would be overestimated when rates are based on leaves while their application in the field is generally as a mixture of leaves and stems. Interactions among plant parts could affect expected patterns of decomposition and nutrient release. According to Mafongoya et al. (1998), if decomposition and nutrient release patterns from a mixture of plant materials reflect the weighted averages of the individual components no interactions occurred; if this is not the case, interactions may have taken place. Interactions between stems and leaves have been reported before and explained as a result of high soluble C in the stems, which caused immobilization of N from leaf tissues (Quemada and Cabrera, 1995).Knowledge of decomposition patterns and rates for different plant materials available on-farm is important for decision making about their optimal use. However, the total amount of nutrients contained in plant materials is critical (Palm, 1995). Results in Table 4 show that the application of dry leaf materials from all species (except CRA) at a rate of 3.75 Mg ha -l can release more than 109 kg ha -l of N and 5 kg ha -l of P, and more than 50, 22 and 10 kg ha -l of K, Ca and Mg, respectively, after 20 weeks of surface application to the soil. Provided that an annual crop like maize can extract close to 80 kg ha -l of N, 18 kg ha -l of P, 66 kg ha -l of K and 15 kg ha -l of Ca and 10 kg ha -l of Mg from the soil (Palm, 1995) we could argue that these leaf materials can potentially supply a considerable proportion of nutrient demand by maize plants. Nevertheless, not all these nutrients would be available to the crop due to potential nutrient losses (denitrification, leaching, etc.), nutrient immobilization by the microbial biomass or simply by incorporation into recalcitrant soil organic matter pools (Vanlauwe et al., 1997a). Our results can be used as indicators of the potential amount and rate of nutrient supply by available options tested in order to improve the nutrient management efficiency of green manure systems in farmer fields. There is great interest in improving synchrony between nutrient release from plant materials and demand by the crop in order to minimize potential nutrient losses and increase nutrient recovery by the crop (Myers et al., 1994).The chemical characteristics of plant materials used as green manures play a fundamental role in the decomposition and nutrient release processes. The judicious management of organic nutrient resources as green manures is dependent on using the right amount and quality of plant material, at the right time.Results from this study are useful to tropical hillside farmers for management of on-farm organic resources based on the potential size of the nutrient additions provided by plant materials as well as timing of nutrient additions to meet crop demand. In order to avoid false expectations about the nutrient supplying capacity of plant materials these should closely represent farmer options. The usefulness of different plant quality indices was assessed as they related to decomposition (i.e. NDF, IVDMD) or nutrient release (i.e. (L+PP)/N for N release). Their utility for screening of potential green manure germoplasm was also discussed.N is usually the most limiting nutrient in tropical soils and considerable efforts have been made to develop alternative or complementary cost-effective practices to N fertilization (Sánchez 1981). Green manures (GM) are considered among these alternative management practices since they can lead to increased soil N availability (Giller and Wilson 1991). A predictive knowledge of GM mineralization patterns, however, is needed for improved nutrient use efficiency in agroecosystems (Constantinides and Fownes 1994;Kass et al. 1997;Giller and Cadisch 1997). While very fast N mineralization rates can be responsible for considerable N losses through leaching, denitrification or volatilization, when N mineralization is very slow little N availability can lead to limitations in crop growth (Myers et al. 1994).The best way to synchronize soil N availability to crop demand is by managing the quantity, quality, timing and placement of plant materials added to the soil (Palm 1995;Mafongoya et al. 1998). The chemical composition of plant materials used (or quality) is one of the most important factors that affect N mineralization rates (Swift et al. 1979;Heal et al. 1997). Plant materials poor in N have limited use in the short term (Constantinides and Fownes 1994) since low N content limits the growth of microorganisms involved in decomposition. The C/N ratio is a useful guide to predict N mineralization patterns. According to Frankenberger and Abdelmagid (1985) C/N ratios greater or equal to 19 limit N availability. Nevertheless, the detection of N mineralization in plant materials with C/N ratios >100 suggest that C compounds such as lignin (L), or polyphenols (PP) can be largely regulating this process (Thomas and Asakawa 1993). According to Palm and Sánchez (1991), the PP content in tropical legumes can play a greater role in N mineralization than N content or the L/N ratio. This is consistent with Tian et al. (1992), who showed that plant materials with low contents of N, L and PP decomposed and mineralized N rapidly. Furthermore, Handayanto et al. (1994Handayanto et al. ( , 1995) ) and Barrios et al. (1997) also found that the (L+PP)/N ratio significantly correlated with N mineralization.This study had the following objectives: (1) to evaluate the effect of foliage from nine GM on soil N availability in a tropical volcanic-ash soil, (2) to determine N uptake by an indicator crop (upland rice), and (3) to relate the quality of plant materials evaluated to both their N supplying capacity and the N uptake by the indicator crop.A glasshouse study was carried out at the Centro Internacional de Agricultura Tropical (CIAT) located at 3°30'N 76°21'W and 965 masl. Glasshouse mean temperature (21°C) and relative humidity (67%) were maintained constant during the whole period of study.GM were selected on the basis of their adaptation to the tropical hillside environment and to volcanic-ash soils, and their differences in plant chemical composition (plant tissue quality). These N concentrations in GM leaves ranged from 2.65% in CAL to 4.63% in MDEE (Table 1). CAL had the highest concentrations of C, acid detergent fiber (ADF) and PP, and highest C/N, L/N, PP/N and (L+PP)/N ratios; while TTH had the lowest contents of C, neutral detergent fiber (NDF), hemicellulose (HEM) and L, as well as the lowest C/N, L/N and (L+PP)/N ratios. IND also had low NDF and HEM contents. CRA had the highest NDF and L contents, and the lowest PP value and PP/N ratio.The volcanic-ash soil used in the experimental pots was collected from the top 20 cm of an Oxic Dystropept (USDA 1998) located in the San Isidro farm (Pescador, Cauca, Colombia) and later passed through a 2-mm mesh. Soil characteristics included: pH (H 2 0) 5.1, 50 g C kg -1 , 3 g N kg -1 , 12 mg NH 4 + -N kg -1 , 42 mg NO 3 --N kg -1 , and 1.1 and 2.5 cmol kg -1 for Al and Ca, respectively. Soil bulk density was 0.8 g cm -3 and P availability was low (4.6 mg Bray-P kg -1 ) as a result of a high allophane content (52-70 g kg -1 ) and high P sorbing capacity (Gijsman and Sanz 1998). Triple super phosphate was added to the soil at an equivalent rate of 50 kg P 2 O 5 ha -1 before establishing the experiment.The experiment was a randomized complete block design with four replicates. The leaves harvested from selected GM were thoroughly mixed and air and oven dried (55±5°C). Dry plant materials were then fragmented into small pieces (<1.5 cm long) and surface applied to 1.5 kg volcanic-ash soil contained in plastic pots at a rate of 100 kg N ha -1 . Three additional treatments were established: urea applications of 50 and 100 kg N ha -1 (FN50 and FN100, respectively) and an unfertilized treatment (FN0) as a control.Soils were capillary-wetted by placing pots on water-filled plastic saucers (Handayanto et al. 1994) so that volumetric moisture content was maintained close to 50%, while leaching was prevented. Fifteen days after plant materials were added five upland rice seeds (Oryza sativa L. var. Oryzica savana 10) were sown in each pot at 1 cm depth. Two weeks after germination rice plants were thinned to two plants per pot. All treatments were evaluated at 2, 4, 8, 12 and 20 weeks after initiating the experiment by carefully removing the remaining decomposing material and sampling the whole soil from the pots. Two samples (20 g) were taken for moisture determination (105°C until constant weight) and the extraction of inorganic N. Soil inorganic N was extracted by shaking the 20 g of soil in 100 ml of 2 M KCl on an endto-end shaker at 150 r.p.m. for 1 h, and filtering through Whatman no.1 filter paper, previously washed with deionized water and 2 M KCl. The resulting soil extracts were then analyzed colorimetrically with an autoanalyzer (Skalar Sun Plus) to determine NH 4 + -N and NO 3 --N contents, and expressed on a dry soil basis (CIAT 1993).At 20 weeks, the fresh weight of aboveground biomass (leaves+stems and panicles) and roots was determined and they were later air and oven dried (55±5°C) for dry weight determination and chemical analysis. Subsamples of each plant material evaluated were analyzed chemically for C, N, ADF, NDF, HEM, L and PP content. In addition, rice plant components sampled at 20 weeks (leaves+stems, panicles and roots) were analyzed for their N content. Dry plant tissues were ground and sieved (1 mm) before analysis. C and N were determined by colorimetry using an autoanalyzer (Skalar Sun Plus). ADF, NDF and L were determined using modified techniques of Van Soest and Vine (Harris 1970) and PP with a modified Anderson and Ingram (1993) method that uses 70% methanol, 0.5% formic acid and 0.05% ascorbic acid as extractant (Telek 1989), the Folin-Ciocalteu reagent and tannic acid as standard. Hemicellulose was calculated by the difference between NDF and ADF.Plant N uptake (milligrams) was calculated by multiplying tissue N contents by tissue dry weights. In order to assess N use efficiency as a function of GM and fertilizer treatments, plant N uptake was expressed as a percent of initial N applied (N recovery) using the following calculation: N recovery (%) = Plant N uptake in treatment -Plant N uptake in control x 100All variables evaluated in soil and rice were subjected to ANOVA. Whenever necessary, variables were root square-transformed to normalize data and homogenize variance. SEs of the difference in means (SED) were calculated from the ANOVA and reported with the data. Correlation analyses were conducted to assess the relationships between quality parameters of GM and soil available N and rice N uptake. SAS (SAS Institute 1989) was used for all statistical analysis.Although fertilized controls (FN100 and FN50) generally produced the highest values of soil available N, considerable quantities of inorganic N were also recovered from soil, after GM application (Fig. 1), suggesting the potential of these plant materials as biofertilizers, as discussed by Palm (1995), Kass et al. (1997), Mafongoya et al. (1998) and Aulakh et al. (2000). GM, however, differed in their impact on soil N availability. Plant materials like MPTL and IND showed high initial soil inorganic N, while MDEE, CRA and CAL had a reduced initial impact on soil N availability, presumably as a result of their higher rates of decomposition and N release (Table 2). A \"priming effect\" on soil organic matter (SOM) mineralization, as a result of N additions (as mineral or organic fertilizers), could also be occurring (Lovell and Hatch 1998). Nevertheless, SOM mineralization in volcanic-ash soils is lower than expected due to the protection of SOM particles in these soils (Gijsman and Sanz 1998).Table 2. Estimated decomposition and N release rates (d -1 ) for initial plant materials added to soil. Data for rate calculations obtained from a 20 weeks litterbag field experiment conducted in Pescador (Cauca). A single exponential model was used to fit the data. (Cobo et al., 2002) Soil NH 4 + levels significantly increased (P<0.01) at week 2, especially in FN100 (111.5 mg N kg -1 soil) and FN50 (81 mg N kg -1 soil), and diminished to almost zero by week 12. Among GM treatments, IND showed the highest value of soil NH 4 + (76.2 mg N kg -1 soil) and CAL the lowest (47.7 mg N kg -1 soil). Conversely, soil NO 3 values after 2 weeks were lower than starting values (i.e. 42 mg N kg -1 soil), except for FN100, but subsequently, at week 4, there was an overall increase in soil NO 3 -, especially in TTH (87.5 mg N kg -1 soil), so that by week 8 soil NO 3 values had surpassed those of NH 4 + . Following this peak, soil NO 3 values also decreased to values close to zero. Total inorganic N [(NH 4 + +NO 3 -)-N)] increased in all treatments during the first 2 weeks of the experiment (Fig. 1). This effect was significantly higher (P<0.01) in FN100 (157.1 mg N kg -1 soil) and FN50 (116 mg N kg -1 soil), while in FN0 we found the lowest value (75 mg N kg -1 soil). Soil inorganic N then followed a declining trend with some treatments showing slightly lower values than FN0 during certain periods (i.e. CAL,CRA and IND at week 4,and MDEE at 8 weeks). This reduction of inorganic N after 4 weeks probably could be the result of rice N uptake and soil N losses, as discussed by Aulakh et al. (2000). Potential soil N losses could be mainly attributed to denitrification since free drainage was prevented by the irrigation system used, and N volatilization is expected to be low in acid soils 1 (Fassbender and Bornemiza 1987). Denitrification may have occurred at microsites because of experimental soil moisture content (50%). On the other hand, the observation that some treatments had lower inorganic N values than FN0 at certain sampling dates suggests N immobilization by soil microorganisms. However, these events were transient and probably due to chemical changes of plant materials to critical levels over time (i.e. higher C/N and L/N ratios).At 20 weeks, plants in FN100, CAL, CAN, MPIT and CRA showed significantly (P<0.01) higher N content than FN0 (83 mg) (Fig. 2). N uptake by rice was highest in FN100 (140 mg), but it was not significantly different from that in CAL (128 mg), CAN (135 mg) and MPIT (114 mg). This observation indicates that these GM could have considerable potential as a source of N to crops in tropical volcanicash soils. N uptake in the other treatments was statistically similar to N uptake in FN50 (98 mg).A more detailed analysis showed significantly higher (P<0.05) leaves+stems and panicle N content in FN100, CAL and CAN than rice plants in FN0. FN100, CAL, CAN, MPIT and CRA also showed significantly higher (P<0.05) root N content than the control. A similar trend was found for plant dry weight. Both plant weight and N uptake were strongly correlated (data not shown).Expressing N uptake as a percent of initial N applied we observed that N recovery by rice plants ranged from 13.1% in MPTL up to about 60% in FN100. N recovery in IND was 20.1% while in CAL it was 47.4%. Plants in FN50 only recovered 15.6% of the N initially applied (Table 3). Likewise, Fox et al. (1990) report a range of 11.2% (Cassia rotundifolia) to 85.1% (Fertilized control) for N recovery by sorghum receiving different legume residues. Aulakh et al. (2000), in a field study using Vigna unguiculata and Sesbania aculeata as GM, reported N recoveries of 60-79% by rice, but 11-16% by wheat. Additionally, our values for C. calothyrsus (47.4%) were higher than those reported by Handayanto et al. (1995) in maize which ranged between 4.2% and 23.1%. The differences in N recovery Total values among studies are probably due to differences in the methodology used (i.e. soil and climate conditions, indicator crop, GM type, form and rate of application, N recovery procedures and evaluation period).Table 3. N recovery by rice from different green manures (GM) and fertilizer treatments after 20 weeks of evaluation. Data are the means of four repetitions. SED SE of the difference in means, FN50 urea application 50 kg N ha-1, FN100 urea application 100 kg N ha-1; for other abbreviations see Relationships among plant tissue quality, soil N availability, and rice N uptake Significant relationships were found between plant quality parameters, soil N availability and rice N uptake (Table 4). Fiber and L content, and C/N, L/N and (L+PP)/N ratios showed a negative relationship with soil NH 4 + -N and total inorganic N [(NH 4 + +NO 3 -)-N)] at 2 and 8 weeks respectively. On the other hand, N and ADF content, and C/N, PP/N and (L+PP)/N ratios correlated to rice N uptake at 20 weeks (Table 4). These results are in agreement with those found by Palm and Sánchez (1991) who observed that 1 and 8 weeks after application of legume leaves to soil, soil inorganic N was significantly correlated with the PP content and the PP/N ratio of the plant material, while the L/N ratio only correlated with soil inorganic N at week 8. On the other hand, Constantinides and Fownes (1994) suggested a significant relationship between N, L, PP, L/N, PP/N, (L+PP)/N and N mineralization from a 16-week incubation experiment using legume and non-legume leaves. Handayanto et al. (1995) also found a significant correlation between N mineralization rates of C. calothyrsus and Gliricidia sepium plant materials with their contents of N, PP, their polyphenol protein binding capacity, and the C/N, PP/N, L/N and (L+PP)/N ratios.Data for soil N availability, N uptake and GM quality, and relationships found suggest that fastdecomposing, high-quality plant materials (e.g. IND) generated high short-term soil N availability but low rice N uptake; while slow-decomposing, lower quality plant materials (e.g. CAL) had a longer-term impact, which resulted in greater N uptake by rice. Reduced performance of higher quality materials could be attributed to the limited synchrony between N mineralization from GM applied and crop uptake. This may be partly explained by the limited effective root system of rice plants before 4 weeks (Fernández et al. 1985), thus missing part of the observed flush of inorganic N at 2 weeks. Therefore, N losses would be expected in treatments generating short-term soil N availability. Pre-sowing surface application of lowquality plant materials (e.g. CAL) and/or surface application of high-quality plant materials (e.g. IND) during periods of high crop N demand (i.e. flowering) could be seen as alternatives for resource poor farmers cropping tropical volcanic ash soils. These practices would increase the agroecosystem nutrient use efficiency and synchrony by reducing potential nutrient losses and increasing N recovery by the crop.ICETEX and CIAT (SWNM project) for financial support during the present research that formed a part of his MSc thesis Table 4. Pearson correlation coefficients and associated probabilities (in parenthesis), according to linear correlation analysis between quality parameters of GM materials added to soil, soil N availability and rice N uptake (n=36). NS Not significant; for other abbreviations see + +NO 3 -)-N)] extracted from soil after 8 weeks of evaluationIn recent years, soil fertility has declined in large areas of the Colombian Andes due to intensive land use. Long-term fallows (6-12 years), needed for soil fertility replenishment, have also virtually disappeared due to increasing population and competing land-use demands. As land use pressures mount, there is a progressive shortening of the fallow period. Hence, the development of technologies that could enhance and accelerate fallow functions and provide a similar level of ecological benefits over a shorter time compared to the natural fallow are urgently needed (Phiri et al., 2001). Such technologies are most likely to be accomplished through the introduction of improved fallow species with fast growing, superior soil conserving and fertility-regenerating properties, and with the ability to control weeds. A useful fallow species must have the ability to sequester nutrients, including P, from soils that have high inherent P reserves but low P availability. Tithonia (Tithonia diversifolia (Hemsfey) A. Gray) is one such species that has been shown to be useful for cycling nutrients via biomass transfer (Nziguheba et al., 1998).Tithonia is a robust succulent non-N 2 -fixing perennial shrub of the family Asteraceae (compositae), which grows 1 to 3 m in height and bears several bright yellow flowers similar to those of the well-known sunflower plant (Helianthus annuus), but the flowers are smaller (about 3 cm in diameter).Tithonia is a native component of natural vegetation in the tropics and subtropics. It grows as a subclimax species that naturally occurs with disturbed soil conditions. Tithonia originated from Mexico, but is now widely distributed throughout the humid and sub-humid tropics in Central and South America, Asia and Africa (Sonke, 1997). It frequently grows wild in hedges, along roadsides, on wastelands and riverbanks, and is common in indigenous fallow systems in Southeast Asia (Jama et al., 2000). It produces large quantities of leaf biomass, and its hedges rapidly grow back after cutting and tolerate repeated pruning. Recently, there has been increasing awareness of the use of Tithonia diversifolia as an indigenous fallow species to improve soil fertility (Niang et al., 1996). Evidence indicates that this species has an ability to accumulate labile soil nutrients, which might otherwise be lost to runoff and leaching, and store them in its rapidly accumulating shoot biomass, which can then be used as a source of plant nutrients or biofertilizers (Nagarajah and Nizar, 1982;Gachengo, 1996;Niang et al., 1996;Jiri and Waddington, 1998;Phiri et al., 2001).Research done by institutions such as Kenya Agricultural Research Institute (KARI), Tropical Soil Biology and Fertility Programme (TSBF) and International Centre for Research in Agroforestry (ICRAF) in the highlands of western Kenya has dramatically raised awareness and expectations of Tithonia green biomass for soil fertility replenishment (Niang et al., 1996). There is also growing interest in the apparent ability of T. diversifolia, probably in association with arbuscular-mycorrhizae (AM), to mobilize and accumulate soil P. Release of P from Tithonia green biomass is rapid, and Tithonia supplies plant available P at least as effectively as an equivalent amount of P from soluble fertilizer (Nziguheba et al., 1998).Tithonia green biomass (green tender stems + green leaves),is relatively high in nutrients when compared to green biomass of other shrubs and trees (Jama et al., 2000). Nagarajah and Nizar (1982) reported nutrient concentration of Tithonia biomass in the ranges of 3.2 to 5.5 % N, 0.2 to 0.5 % P and 2.3 to 5.5 % K based on the analysis of 100 dry samples of green biomass in Sri Lanka. The mean values of nutrient concentration of green leaves of Tithonia collected in East Africa are 3.5 % N, 0.37 % P and 4.1 % K on a dry-weight basis (Jama et al., 2000). The concentration of N in Tithonia green biomass is comparable to that found in N 2 -fixing leguminous shrubs and trees, whereas the P and K concentrations are higher than those typically found in shrubs and trees (Jama et al., 2000;Phiri et al., 2001). Tithonia biomass is also high in nutrients other than N, P and K. Gachengo et al. (1999), for example, found 1.8 % Ca and 0.4 % Mg per unit dry weight of the green Tithonia biomass.Tithonia diversifolia is deliberately being introduced into mid-altitude hillside agriculture system in Colombia to enhance soil fertility (in a chemical, physical and/or biological sense) and to some extent to suppress weeds (Phiri et al., 2001). Compared with natural fallow, Tithonia markedly improved the availability of several essential nutrients, particularly P and K (Phiri et al., 2001). Jama et al. (2000) reported that the biomass production of Tithonia is influenced by establishment methods, frequency of cuttings, stand density and site conditions. To facilitate rapid establishment of Tithonia on a large scale, there is a need to investigate the effect of its establishment method on soil properties and plant growth attributes. Tithonia propagates from seeds that frequently germinate naturally under its canopy. Seedlings can be dug up and transplanted elsewhere. However, when Tithonia is established from seeds in the field, germination can be poor, especially if the seeds are sown deep or covered with a clayey soil (Jama et al., 2000). Under field conditions, Tithonia is more easily established from stem cuttings than from seeds (King'ara, 1998). The main objective of the present study was to determine the effect of method of establishment (vegetative stem cuttings versus bare-root seedlings-here called plantlets) of Tithonia diversifolia on shoot and root growth characteristics, AM association, nutrient acquisition and utilization, and P dynamics in soil.This study was carried out at CIAT's \"San Isidro\" experimental farm in Pescador located in the Andean hillsides of the Cauca Department of southwestern Colombia (2º 48′ N, 76º 33′ W) at 1505 m.a.s.l. The area has a mean temperature of 19.3 °C and a mean annual rainfall of 1900 mm (bimodal).The plots had a slope of approximately 30 %. The soils, derived from volcanic ashes, have been classified as Oxic Dystropepts (Soil Survey Staff, 1998), having the following characteristics: pH (H 2 O) 5.1; 50 mg g -1 C; 3 mg g -1 N; 4.6 mg kg -1 soil of Bray-P; and 1.1 and 2.5 cmol kg -1 soil for Al and Ca, respectively (Cobo et al., 2000). The soil has a medium to fine texture (45 % sand, 27 % silt and 38 % clay) (IGAC, 1979) of high fragility and low cohesion with shallow humic layers. Low soil P availability is presumably the result of high allophane content (52-70 g kg -1 ), which increases its P sorbing capacity (Gijsman and Sanz, 1998).The two treatments used were one-year-old Tithonia diversifolia (Hems.) Gray (1) bare root seedlings (plantlets) and (2) vegetative stem cuttings (stakes). Tithonia stakes, 20-40 cm long with 4 or 5 nodes, were cut from mature plants, planted at a slanting angle of 45-60 degrees with 1 or 2 nodes below the ground level to leave 2 or more nodes above the ground. The two propagating materials were planted at the same plant density (40 000 plants/ha at a staggered spacing of 50 cm x 50 cm) in 20 x 20 m plots. Three one cubic meter monoliths, each including one Tithonia plant, were randomly collected within each treatment plot. The experiment was laid down as a randomized complete block (RCB) design with establishment method as treatment.After one year of plant growth, a sample area of 1 m 2 was randomly selected within each plot and all the above ground biomass in this area was harvested. The biomass from the rest of the plot was harvested for the total biomass determination. The biomass from the sample area was separated into leaves, stems and the reproductive structures (flowers and seeds). The leaves were used for determination of leaf area index, and the leaves, stems and reproductive structures were analysed for N, P, K, Ca and Mg. An area of 0.5 x 0.5 m was selected within the sampling area, and all the soil from the 0-5, 5-10, 10-20, 20-40, 40-60 cm soil depths was collected for root and AM determinations. These samples were air-dried and visible plant roots were removed and then gently crushed to pass through a 2-mm sieve. The <2-mm fraction was used for subsequent chemical analysis.The leaf area (cm²) was determined by measuring fresh leaves with an LI 3000 Area Meter (LI-Cor Inc., Lincoln, NE). The leaf area index (LAI, m² of leaf area per m² of ground area) and the specific leaf area (SLA, m² of leaf area per kg of dried leaves) were calculated. Measurements of photosynthetic efficiency of intact leaves were made with a portable Plant Efficiency Analyzer (Hansatech, King's Lynn, UK). Leaves were dark adapted for 20 min using leaf clips before a 5-s light pulse (1500 μmol m -2 s -1 ) was supplied by an array of red light-emitting diodes The rapid turn-on of the light-emitting diodes allowed the accurate determination of Fo (minimal fluorescence intensity with all photosystem II reaction centers open while the photosynthetic membrane is in the non-energized state in the dark) and, hence, Fv (maximum variable fluorescence in the state when all non-photochemical processes are at a minimum, i.e., Fm-Fo) (Kooten and Snel, 1990;Sundby et al., 1993). The ratio of variable to maximal fluorescence (Fv/Fm = (Fm-Fo)/Fm) (Fm = fluorescence intensity with all photosystem II reaction centers closed) is a measure of the maximal photochemical efficiency of photosystem II.Root distribution was determined using soil coring method (Rao, 1998). For each replication, a total of 12 soil cores at different soil depths (0-10; 10-20; 20-40; and 40-80 cm) were collected 10 cm from the base of the plant across the row. After washing out the roots on a 1 mm sieve, the \"live\" roots were hand separated from organic material. Root length was measured with the Comair Root Length Scanner (Commonwealth Aircraft Corporation, Melbourne, Australia) and expressed in km of root length per m² of ground area. Root biomass was determined after drying the samples in an oven at 70 °C for 2 days. The specific root length was calculated in m of root length per g of dried roots. A number of other plant attributes were determined including nutrient status of plant parts, shoot nutrient uptake, nutrient uptake efficiency (μg of uptake in shoot biomass per m of root length), and nutrient use efficiency (g of shoot biomass production per g of total nutrient uptake) (Salinas and Saif, 1990;Rao et al., 1997).Mycorrhizal association was assessed by the number of spores per 100 g soil and AM root infection percentage in coarse and fine roots according to the method of Sieverding (1991). To separate spores from the soil, a 50 g sample of well-mixed soil, was suspended in water for 1 min (for sedimentation of coarse sand), and then the suspension was decanted over a series of soil sieves (a sieve with 0.350over one with 0.125over one with 0.045-mm mesh size). Suspending and decanting were repeated three times. Root material in the top sieve was carefully washed with water and then transferred with a little water to a petri dish. The contents of the medium sieve and of the finest sieve were separately transferred to 100-ml centrifuge tubes. In the tubes, the sievings were brought into suspension in 30 ml water and 30-40 ml of a sugar solution (70 g sugar dissolved in 100 ml water) was injected into the bottom of the tube with the aid of a 50-ml syringe so that a gradient was established in the centrifuge tube. The sample was centrifuged (with a centrifuge with swinging bucket and horizontal head) at 1000 revolutions per min. for 10 min. During this process soil particles settle on the bottom and spores remain on the surface of the sugar gradient. Spores were extracted with syringe from the gradient and placed in a clean sieve with 0.045-mm mesh opening; then the spores were washed with water for 2-3 min. before being transferred in water to a petri dish. Spores in the root fraction and the centrifuged samples were observed and counted under a stereomicroscope at 40x magnification.To determine AM fungal root infection, the soil was immersed in a tub of water and gently agitated to separate the roots from the soil. The roots were separated into coarse (> 2mm diameter) and fine roots. The roots were then washed with water using a hose over a 1-2 mm screen (to catch roots). The roots were then transferred to a flask and heated in 5% KOH at 90 °C for 15 min. The KOH was then rinsed off the roots with water on a fine sieve. Roots were stained in acidic glycerol/trypan blue for 15 min at 90 ºC and then destained and stored in 50 % acidic glycerol and subsequently used for determination of AM root infection using the modified grid intersect method (Newman, 1966).A sequential P fractionation as per the method of Tiessen and Moir (1993) was carried out on 0.5g sieved (<2-mm) soil samples. In brief, a sequence of extractants with increasing strength was applied to subdivide the total soil-P into inorganic (P i ) and organic (P o ) fractions. The following fractions were included: (1) resin P i extracted with anion exchange resin membranes (used in bicarbonate form) was used to extract freely exchangeable P i . The remaining P o in the extraction from of the resin extraction step (H 2 O-P o ) was digested with potassium persulfate (K 2 S 2 O 8 ) (Oberson et al., 1999). (2) Sodium bicarbonate (0.5 M NaHCO 3 , pH = 8.5) was then used to remove labile P i and P o sorbed to the soil surface, plus a small amount of microbial P (Bowman and Cole, 1978). (3) Sodium hydroxide (0.1 M NaOH) was used next to remove P i more strongly bound to Fe and Al compounds (Williams and Walker, 1969) and associated with humic compounds (Bowman and Cole, 1978). (4) HCl P i was obtained by extraction with 1.0 M HCl; (5) HCl hc-P and -P o were extracted with hot and concentrated HCl; and (6) residual P was obtained by digestion with perchloric acid (HClO 4 ). To determine total P in the NaHCO 3 and NaOH extracts, an aliquot of the extracts was digested with K 2 S 2 O 8 in H 2 SO 4 at >150 °C to oxidize organic matter (Bowman, 1989). Organic P was calculated as the difference between total P and P i in the NaHCO 3 and NaOH extracts, respectively. Inorganic P concentrations in all the digests and extracts were measured colorimetrically by the molybdate-ascorbic acid method (Murphy and Riley, 1962). All laboratory analyses were conducted in duplicate and all the data are expressed on an oven-dry weight basis.Analyses of variances were conducted (SAS/ STAT, 1990) to determine the significance of the effects of method of Tithonia establishment on soil properties and plant growth attributes. Planned F ratio was calculated as TMS/EMS, where TMS is the treatment mean square and EMS is the error mean square (Mead et al., 1993). Where significant differences occurred, least-significant-difference (LSD) analysis was performed to permit separation of means. Unless otherwise stated, mention of statistical significance refers to α = 0.05.Establishment of Tithonia by plantlets resulted in significantly greater mycorrhizal root infection (P=0.05) in both coarse and fine roots, as compaewd to Tithonia established by stakes and the differences were of 21 and 31%, respectively (Table 1). The corresponding difference in the number of spores per 100 g of soil was 30% but the difference between the two methods was not statistically significant (P=0.05). The higher AM infection of plants established with plantlets could have contributed to the greater acquisition of nutrients (Table 2) observed under this treatment. Increased mycorrhizal uptake of simple forms of organic P (P o ) (Jayachandran et al., 1992) and increased net release of P from organic matter due to uptake by mycorrhizal hyphae (Joner and Jakobsen, 1994) have been demonstrated. Although the source of soil P is envisaged to be the soil solution and to be the same for both roots and hyphae, the transfer across the symbiotic interface results in increased nutrient acquisition by the plant (Smith and Read, 1997). This is because mycorrhizal hyphae, due to their small size and spatial distribution compared to roots, are able to penetrate soil pores inaccessible to roots resulting in exploitation of a larger soil volume for nutrient acquisition, particularly of non-mobile nutrients such as P, Zn and Cu (Smith and Read, 1997). Rhodes and Gerdemann (1975) demonstrated the ability of the hyphae of mycorrhizal fungi to absorb 32 P from a distance as much as 7 cm away from the roots. The kinetics of P uptake into hyphae may differ from that of roots. The fungal membrane transport system seems to have a higher affinity for phosphorus (lower K m value) than roots (Cress et al., 1979), leading to more effective absorption from low concentrations in the soil solution and possibly lower threshold values below which uptake ceases (Smith and Read, 1997). The work with cassava by Yost and Fox (1979) illustrates this point. This species appears to have a very high P requirement, coupled with a very inefficient P uptake system in the absence of mycorrhizal colonization. Despite this, cassava is well known for its growth on soils of low fertility and its efficiency of uptake is markedly increased when roots are colonized by mycorrhizal fungi (Smith and Read, 1997). The increased efficiency of the plantlet to associate with mycorrhizae may be related to the initial physiological competence of the plantlet compared to the vegetative stem cutting (stake). Plantlets have all the basic components of a mature plant and are able to start photosynthesis shortly after transplanting.Plantlets are also likely to associate with AM faster than cuttings because they already have roots and produce photoassimilates that are an essential component for an effective plant-mycorrhizal symbiosis. This symbiosis is likely to proliferate rapidly once established. Meanwhile, the stakes have to initiate root and shoot growth before they can associate with AM resulting in a time lag for symbiosis to be established. How long this lag period lasts is unknown. Tithonia established by plantlets had a total shoot biomass of 16.5 t/ha, which was significantly higher (P<0.05) than the 7 t/ha under vegetative stem cutting (stake) establishment (Table 2). The total root length and root biomass were not significantly affected by the method of establishment, although, on average, plants established by plantlets had greater root biomass, root length and specific root length indicating that Tithonia under this method of establishment had developed a finer root system. It is generally observed that thicker roots may be more favorable for mycorrhizal association (St John, 1980). It appears that in the case of Tithonia both thick and fine roots were colonized by mycorrhizae. Tithonia plant established by using plantlets had significantly higher shoot uptake and use efficiency of N, P, K, Ca and Mg (Table 2). The higher values of these attributes in plants established using plantlets could be attributed to greater mycorrhizal (AM) colonization under this establishment method, which might have increased the effective volume for nutrient uptake. There is evidence that nitrogen (N) is taken up by AM hyphae from inorganic sources of ammonium (Ames et al., 1983). Any direct effect of AM on NO 3 -uptake is not known (Sieverding, 1991). Potassium (K) and Mg are often found in higher concentrations in mycorrhizal than non-mycorrhizal plants, although a direct transport of K and Mg in AM is not confirmed (Sieverding, 1991). Some experimental work suggests that in K-deficient soils the improved K uptake is related to the AM fungal species and that K may be transported by AM fungal hyphae (Sieverding and Toro, 1988). Calcium (Ca) transport in AM hyphae is not clearly confirmed; the Ca uptake is apparently affected by interaction with other elemental nutrients. However, it should also be noted that this improvement in nutrient acquisition could be as a result of relief from P stress and possibly from the uptake of some essential micronutrients. These processes will result in general improvement in growth, thus indirectly affecting the uptake of other nutrients. The differences between mycorrhizal and non-mycorrhizal plants usually disappear if the latter are supplied with a readily available P source (Bethlenfalvay and Newton, 1991;Azcón-Aguilar andBarea, 1992, Barea et al., 1992;Bethlenfalvay, 1992).Available P (Bray-II) in the 0-5 and 5-10 cm soil depth was significantly greater in plots where Tithonia was established by plantlets (Table 3). The plantlet method resulted in significantly higher Ca and Mg in the profile up to 20-cm soil depth (Table 3), and a lower content of exchangeable Al (results not shown for brevity). These differences may be related to the differences in mycorrhizal associations between the plantlet and stake establishment methods. Bowen (1980) and Jehne (1980) reported that AM might play an important role as transport paths for nutrient cycling processes. AM-root external mycelia presumably can efficiently and intensively extract nutrients from a greater soil volume and thus reduce the amount of solubilized or mineralized nutrients that are chemically fixed or leached. This function of AM fungi was concentrated in the 0-20 cm depth of the soil profile where most root growth occurred. Biologically available P (H 2 O-P o , resin-P i , and NaHCO 3 -P i and -P o ): The biologically available P consists of labile P and represents soil solution P, soluble phosphates originating from calcium phosphates, and weakly adsorbed P i on the surfaces of sesquioxides or carbonates (Mattingly, 1975). The resin P i and the NaHCO 3 -P i are considered readily available for plant uptake. At soil layers of 0-5, 5-10 and 10-20 cm, the resin P i was significantly higher under the plantlet establishment method (Table 4). The resin P decreased sharply with increasing soil depth and accounted for 0.4 % and 0.07 % of the total soil P at the 0-5 and 40-60 cm soil layers, respectively. The NaHCO 3 -P i was higher under the plantlet establishment method; however, the differences were not significant except at 10-20 cm soil depth. Similar to the resin P, the NaHCO 3 -P i decreased sharply with increasing soil depth and accounted for 4.5% and 0.4% of the total soil P at the 0-5 and 40-60 cm soil layers, respectively. The organic fractions of the bioavailable P include the H 2 O-P o and NaHCO 3 -P o , which is considered \"readily mineralizable\" and contributes to plant-available P (Fixen and Grove, 1990). This P o fraction includes nucleic acid-P, sugar-P, lipid-P, phytins, and other high-molecular-weight P compounds (Bowman and Cole, 1978). The H 2 O-P o contribution to the total soil P was very small and decreased steadily with depth. The plantlet establishment method had a higher H 2 O-P o at the 0-5 and 5-20 cm soil depths. The NaHCO 3 -P o was on average 4% of the soil total P. The method of establishment of Tithonia did not affect this fraction. The absence of an effect of the establishment method on NaHCO 3 -P o is consistent with results by Tiessen et al. (1992), who found that NaHCO 3 -P o was relatively constant in shifting cultivation systems on an Oxisol. The sum of all the fractions making up the bioavailable P (H 2 O-P o + resin-P i + Total NaHCO 3 P) was less variable and was about the same under the two establishment methods. It decreased with increasing soil depth and ranged from 56.4 (0-5 cm) to 18.0 µg/g (40-60 cm), which was between 4 to 9% of total P (Table 4; Fig. 1). Moderately resistant P (NaOH-extractable P): This fraction is thought to be associated with humic compounds, and amorphous and some crystalline Al and Fe phosphates (Bowman and Cole, 1978). The NaOH (0.1 M, pH = 8.5) used completely solubilize the synthetic iron, aluminum phosphate and any labile-P o (Anderson, 1964). A large proportion of P was recovered in this fraction, where the total NaOH (P t ) represented between 37 % and 18 % of the total soil P at the 0-5 and 40-60 soil layers, respectively (Fig 1). The plantlet establishment method resulted in high NaOH-P i , however, the results were not significant (Table 4). The effect of the establishment method was variable on the NaOH-P o fraction and did not follow any particular trend with increase in soil depth.The sparingly available P includes the 1M HCl, the hot-and-concentrated HCl (P i and P o ) and the Hedley et al. (1982) residual-P. The dilute HCl (1M) acid extractant is used to dissolve acid-soluble P, which consists of relatively insoluble Ca-phosphate minerals such as apatite (Williams et al., 1980). This fraction is clearly defined as Ca-associated P, since the Fe-or Al-associated P that might remain unextracted after the NaOH extraction is insoluble in acid. There was rarely any P o in this extract. On average the dilute HCl-Pi represent about 1 % of the total soil P and was only significantly affected by the establishment method at 5-10 and 10-20 cm soil layers. It increased sharply with increasing soil depth from the 5-10 to 40-60 cm soil layers. The hot concentrated HCl is useful for distinguishing P i and P o in very stable residue pools. The P o extracted at this step may also simply come from particulate organic matter that is not alkaline extractable, but it may be easily bioavailable. The plantlet establishment method resulted in a significantly higher HCl hc-P i at the 0-5, 5-10 and 10-20 cm soil layers (Table 4). The HCl hc-P o showed a tendency to be greater under the plantlet establishment method, but was only significantly different from the stake establishment method at 5-10 cm soil layer. The residual P is thought not to be available on a short time scale such as one or two crop cycles, but a small fraction of this pool may become available during long-term soil P transformations. The residual P represented a high proportion of the total P and was significantly affected by the establishment method at the 0-5 cm soil layer. This fraction decreased steadily with increasing soil depth.This study has shown that the better method of establishing Tithonia as a fallow species in volcanic-ash soil is the use of bare root seedlings (plantlets) in comparison to vegetative stem cuttings (stakes). Establishment by bare root seedling resulted in increased plant growth and nutrient acquisition, which are desirable plant attributes for fallow systems because of enhanced nutrient cycling.Ames R N, Reid C P P, Porter L K and Cambardella C 1983 Soil erosion is a major problem worldwide. Climatic impacts aside, the main reasons for soil erosion are both, inappropriate land-use and improper fertilizer management, (Lal and Stewart, 1990;Oldeman, 1990;El-Swaify, 1991) as well as socio-economic constraints (Steiner, 1994, Mueller-Saemann, 1998 et al.). In the process of acquiring a basic knowledge of soil degradation, efforts have focused on structural changes at the soil surface (Sumner and Miller, 1992;Sumner and Stewart, 1992;Bresson, 1995;Valentin and Bresson, 1998)). Recent observations indicate that the physical and chemical degradations of soils in the Andean zone are related to the phenomena of soil crusting and sealing.Soil crusts are thin layers of hardened soil on the surface, occurring on dry soils (Roth, 1992;Bresson, 1995). The term \"soil sealing\" is used to describe superficial impermeabilities mainly occurring in wet circumstances. Soil sealing occurs if dissolved aggregates infiltrate in the soil pores leading to compact soil horizons and thus reducing infiltration (Scheffer-Schachtschabel, 1998). Both phenomena negatively impact water infiltration, and reduce air permeability and seedlings' emergence (USDA, 1996, Bajracharya et al., 1996, Le Bissonnais, 1990). Due to the reduction of water infiltration, the surface runoff increases; resulting in enhanced soil erosion and reduced harvest yield.The soil crust development of Andean soils of volcanic origin is not yet well understood. Therefore, the aim of this work is to characterize the phenomenon of soil crusting on Andean Inceptisols. This project is supported by special project funds from the DAAD/Germany, the Eiselen Foundation/Germany, the BMZ/Germany and the University of Hohenheim/Germany.Field research was conducted at the Santander de Quilichao Research Station, Dep. Cauca of Colombia (3°6'N, 76° 31' W, 990 m.a.s.l). Trials had been installed on an amorphous, isohyperthermic oxic Dystropept (Inceptisol), developed from fluvially translocated partly weathered volcanic ashes. The field site has a bimodal rain distribution with two maximas in April-May and October-November, with a mean annual rainfall of 1799 mm, a rain intensity up to 330 mm/h and a mean annual temperature of 23.8°C. The measurements of soil crusting have been made on 27 Standard Erosion Experimental Plots. These plots, originally designed by the soil conservation team from the University of Hohenheim as completely randomized blocks in three repetitions, have been used since 1986 (Table 1). They were sampled at 0 to 5cm depth. The treatments from December 1999 are described in Table 2. Before planting, the experimental plots have been limed with dolomitic lime (500 kg/ha) and plots with mineral fertilizer have been fertilized with 300 kg/ha mineral fertilizer (10N-30P-10K). Chicken manure from a local poultry farm had the following nutrient content (N: 3.43%, P: 1.82%, K: 2.73%, Ca: 3.32%, Mg: 0.64%, Fe: 1364 ppm).To quantify and describe soil crusting and sealing, different measurement tools have been used in the field.After planting Cassava in December 1999, field measurements with a Pocket Penetrometer (Model DIK-5560) were carried out.Besides pentrometer measurement, a Hand Vane Tester (Model EL26-3345) was used to measure shear strength at the soil surface. Both tools were used weekly, each Penetrometer measurement 24 times and Torvane measurement 6 times per plot.To describe direct effects of soil crusting and sealing on infiltration, a mini-rainsimulator was used in the field. Infiltration was measured by irrigating a defined soil area (32,5cm x 40cm) with a special amount of rain (90mm/h). The construction of this mini-rainsimulator enabled to subsample runoff periodically (every 5 min). The difference between irrigated amount of rain water and run-off data is defined as infiltration.Cassava root yield in December 2000 was measured after harvest to determine the impact of soil compaction process. Penetrometer and Torvane Results of Penetrometer and Torvane measurement are presented in Figures 1. During the wet season, penetration resistance was similar in all treatments. At the beginning of the dry season in May/June, differences between treatments were noted. Notably, the Cassava + 8 t/ha chicken manure became a hard soil (penetration resistance 25,4 kPa, shear strength 67 kg/cm²). Over time the minimum tillage plot generally became harder than other plots, but the well-developed and stable aggregate structure prevented negative impact on water infiltration (see below). The high amount of chicken manure caused a dispersion of clays in the wet season and results in uniform clods after drying. It was noticed that the Cassava monoculture and Cassava intensive tillage tended to be extremely soft, thus building up a singlegrain structure also called pseudo-sand. Torvane measurement data tended to be similar to penetrometer measurement. Figure 1 indicates the increase in shear strength in the dry season especially within treatments of Cassava + 8 t/ha chicken manure.In general, all treatments except the Cassava intensive tillage treatment had a high shear strength from June-July and turned from 13 -22 kg/cm 2 in the wet season up to 43 -76 kg/cm 2 in the dry season.Results are presented in Figure 2. Cassava + 8t/ha Chicken manure had the lowest infiltration after 55 minutes with a final infiltration capacity of 36 mm/h. It has to be emphasized that Cassava min. tillage as well as Cassava rotation treatment had both an excellent infiltration capacity. Minimum tillage influenced the soil structure positively in the way that aggregation over a long time period is supported. This helped to build up a soil structure, as also the mulch at the surface led to a better infiltration.Results of harvest data are presented in Table 3. Overall, the best root yields were found in Cassava 4t/ha chicken manure and Cassava rotation. High Cassava root yields in these treatments are due to improved soil conditions such as moderate soil hardening, sufficient fertilization, enhanced soil aggregation and high water infiltration. In contrast, the lowest yields were found with Cassava monoculture and Cassava intensive tillage treatments. The Cassava monoculture treatment is characterized by a low nutrient content in the soil through insufficient fertilization over a long period of time. The single grain structure and low infiltration capacity contributed to low root yield. The Cassava intensive tillage treatment is characterized by a breakdown of the pore system. Thus, leading to a lack of infiltration and reduced yields. In both treatments, roots were very small and economically worthless. Cassava 8 t/ha chicken manure had high amounts of plant biomass but hard soil structure, preventing optimal development of Cassava roots. In Cassava minimum tillage treatment, root growth was limited to the area loosened before planting. Therefore yields in both treatments were lower than in Cassava rotation and Cassava 4 t/ha chicken manure In summary, penetration resistance and shear strength showed no risk of structural damage in the wet season. This worsened in the dry season when Chicken manure treatment turned into hard and impermeable soils. Although, the minimum tillage treatment had high penetration resistance and high shear strength values, this caused no deterioration because of a good aggregation status. This can clearly be seen in the results of infiltration measurement. Monoculture and intensive tillage had neither high penetration resistance nor high shear strength. In contrast, these treatments easily built up the so-called pseudo-sand that lead to high proportions of small aggregates, and thus to high amounts of soil erosion. The more modern techniques of Minimum tillage and Cassava rotation had the best and most sustainable status. Those treatments had a good aggregation, showed adequate infiltration rates and did not suffer from human induced fertilizer damage, e.g. soil hardening due to chicken manure or deterioration of soil matrix through intensive tillage. Chicken manure, especially 8 t/ha, had a severe impact on soil surface.Further research is needed to specify the reasons why chicken manure has such an influence on aggregates. It is unclear which dispersion agent might be that leads to aggregate dispersion. Furthermore, structural changes through intensive tillage or minimum tillage have to be looked at more closely in order to ascertain how severely aggregate breakdown affects plant growth on Inceptisols.Results from penetration and shear strength measurement showed the marked influence of chicken manure on soil structure. Chicken manure generally resulted in a deterioration of soil's structural status. A reduction of infiltration, especially in chicken manure plots, substantiates the hypothesis that inappropriate fertilizer management is one of the key factors in structural deterioration on Inceptisols. Dispersion of clays, generally cited as the main reason for soil sealing, is influenced by the impact of chicken manure. Further research will need to focus on the impact of fertilizers on the soil surface in order to design sustainable land-use systems for Andean hillside farming.Increasing understanding of local ecological knowledge and strengthening interactions with formal science strengthened. J.J. Ramisch and M. Misiko TSBF-CIAT, PO Box 30677, Nairobi, Kenya Rationale: The project is testing a community-based interactive learning approach, which aims to improve and sustain agricultural productivity by facilitating a common understanding between scientists, farmers and other stakeholders about how agro-ecosystems operate and how best to manage them.The major goal of the project is to develop innovative and interactive learning tools to facilitate the exchange of knowledge and skills between farmers, scientists and other agricultural knowledge brokers. The specific focus of the project is to broaden farmers' soil fertility management strategies by incorporating scientific insights of soil biology and fertility into their repertoire of folk knowledge and practical skills.A parallel goal is to strengthen the understanding of indigenous agro-ecological knowledge among scientists, extensionists and other stakeholders and to elucidate the local realities and complexities that determine farmers' decision making. This interactive and multidirectional communication process provides opportunities for both farmers and scientists to question and validate their knowledge. It also presents a mechanism for disseminating and sharing useful local knowledge between different groups of agricultural stakeholders.The major activities of the first year were largely exploratory in nature, covering three main areas: 1) community studies and learning activities (this Activity section), and 2) development of methodologies for the research and for farmers to share information with each other and with researchers, and 3) monitoring and documentation (for both, see Activity 2.2 \"Community-based learning and dissemination strategy developed\"). The coming year will see much more emphasis on communication strategies, building on existing knowledge, and broadening the scope of farmer-to-farmer exchanges.Community studies and learning activities: The four study sites all have some previous exposure to either TSBF or local NGO's that had worked on soil fertility management. They cover a range of agroecological conditions and ethnicities, and thereby present an interesting and representative diversity of communities in Western Kenya (Box 1).The project began with introductory, community discussions, which led into exploratory group work to assess the types and extent of knowledge and assumptions held locally about soil fertility and soil ecological processes. Once this baseline study of 'folk ecological' knowledge was completed, there were The introduction of the project centred on community interviews held in the four sites. These events, facilitated by a multi-disciplinary team had as their objectives:• Determining the local \"vocabulary\" used for discussing soil fertility • Identifying concepts locally related to soil fertility knowledge (classification, process, relationships) • Identifying the elements of locally understood \"common sense\" related to soil fertility • Identifying the individuals or groups who possess specialised knowledge of soil fertility and its management • Identifying the assumptions or \"rules\" of local soil fertility knowledge.Following the initial meetings, farmers and researchers alike were eager that findings be returned to initial groups for discussion and validation. The collective findings of the community interviews were synthesised and presented back to the communities in open seminar events, which led to follow up activities on locally important themes. In particular, transect walks and other ground-truthing activities helped both broaden the involvement of community members beyond the participants of the initial meetings and to build rapport with potential key informants with specialist knowledge.Key findings from the baseline study activities include:• Local soil types were readily identifiable. Local descriptions distinguished more soil types than were recognised as distinct soils by scientists. Soil maps are based on 'expert opinion' but do not reflect the high familiarity and local knowledge of farmers in daily contact with their land. Individual farmers also adapt common local names to the soils found on their own land. • Soil names reflected features of the surface layer: colour, texture, depth, fertility, erosion, first user or settler (i.e. history). Soil was understood holistically, as \"mother\", \"ourselves\", \"life\", or \"wealth\", and not just as a physical surface on which life is found. The soil was more commonly acknowledged as the source of life and wealth rather than alive or a type of wealth in its own right.• Farmers identified a diversity of directly observable, constituent parts of soil (living and nonliving), including minerals, sand, silt, decaying things, worms, insects, moisture, and temperature. The presence of invisible or microscopic aspects of the soil was observed indirectly, through the growth of specific wild plants, or through crop performance.• No single local terminology exists to describe soils' fertility status, and there was no significant gender difference in the use vocabulary or concepts. A linguistic difference was that the Teso word \"aboseteit\" referred to soil fertility and things that enhance it, while Luyia used a more general word \"obunulu\" to denote both a fertile soil and rich, fatty meat.• Multiple analogies were used when describing soil fertility, including paired opposites like \"healthy / sick or hungry\", \"strong / weak or tired\", \"young / old\", \"moist / dry\". The aspects considered important in describing fertility were texture (light, loose soils were preferred to heavier ones, which would stick on implements), colour (darker soils were considered more fertile), health or energy (as seen in crop performance, \"weak\", \"old\", or \"tired\" soils need to rest or to be fed).• Many locally known plants indicate high or low soil fertility. These indicator species, however, are not universal and their interpretation may vary. The presence of certain uncommon species may be enough to imply \"high\" fertility, while the relative performance of widespread species is often compared to give an indication of fertility. Generally, indicator species appear to reflect \"inherent\" soil properties more than trends of improvement or decline. Knowledge of plant indicators is both widely accepted and highly debated, and will be investigated further. In particular, the distribution and use of this knowledge is being more intensively studied by the Master's student Nelson Otwoma (see \"Training\" below).• Respondents assumed that without inputs soils become \"poor\" or \"worthless\". It was also widely believed that using inorganic fertilisers encourages crops to overexploit the soil's energy and can quickly \"exhaust\" or \"bleach\" the soil. Because organic inputs have longer residual effects than inorganic ones, respondents felt that both must be used in combination, or one will disrupt a desired balance of elements in the soil.• Applying \"farmyard manure\" and constructing terraces were the most common soil management interventions. There was extreme individual variation between farmers in terms of what materials were included in \"manure\" and the manner in which they were managed while decomposing or applied to cropland. Manure management will be a major topic for further investigation. Only farmers in Aludeka did not commonly use manure, since land is relatively abundant and trypanosomiasis limits cattle keeping.Major changes in managing soil fertility over the last fifty years include the introduction of inorganic fertiliser, construction of terraces, systematic use of livestock manure, fallow trees and compost. Traditional farming encompassed fallowing, shifting cultivation and slash-and-burn as major practices. Practices that were introduced by the government and had been or were being abandoned include crop rotation on an annual basis, since land is too limiting. The follow-up activities with key informants have particularly emphasised participant observation of management practices, which are notoriously difficult to discuss in the abstract and are more meaningfully observed on the ground.Traditionally, information was disseminated communally and government did not have a role in provision of services like agricultural extension. Farmers learned mainly through observation, apprenticeship and experience, resources were abundant and knowledge on the environment was extensive. Today, farmers have more knowledge on intensive agriculture but use of this knowledge is constrained by limited access to key resources, including land, biomass and livestock. Many farmers reported that reduced landholdings and the difficulty of acquiring new land limit their ability to fully exploit their traditional knowledge of soils and their management. As a result of land scarcity, there was little correspondence between soil type and crops grown, even when farmers stated that a given soil was not well suited to the crop being grown. Resource constraints will almost certainly limit the relevance and amount of traditional knowledge being passed on to later generations.Usually, information about meetings and other research events is given out to relatively few farmers. This information later reaches their friends and also neighbours and relatives. In addition, most of such events are held in the open where most passers-by see. Nevertheless, some farmers did not feel encouraged to attend these events. As an example, a woman who lives adjacent to a TSBF research plot in Emuhaya said: \"I would like to attend research events, …but I have no one to 'follow' (i.e. orientate her to them)\". She was aware that research on soil fertility had been continuing for long in her village. She also knew it would be beneficial but had never regarded herself to be part of the process.Specifically relating to dissemination of knowledge on soil fertility, there was a common feeling amongst participants in the four sites was that there was inadequate awareness creation on soil fertility research. Many farmers did not understand how they would participate or even directly gain. It is as a result of this that many farmers still expect money or other handouts from researchers. Farmers suggested some steps that could be useful in enhancing the spread of knowledge on soil fertility:• Experimental and demonstration plots should be soil-based; located in different soil types found in the study areas, which would assist farmers to relate the practices to their situation easily.Participants observed that some trial plots may have performed better than others due to differences in soils and that some preferred practises would be inapplicable in certain soils. At present, TSBF hires trial plots depending on their availability, adequacy of size and shape of trial plot, willingness of farmers to rent their plots out and to co-operate, security, accessibility, absence of such barriers as rocks and termite mounts, representativeness of agro-ecological zones. • Plots should bear well-labelled posters showing procedures on experiments and stating that it is pure \"trial\" and not something automatically beneficial or \"interesting\" to farmers. One farmer suggested that trial plots that are managed by the researcher should be hidden from busy roads so that they are not seen by passers-by especially when they perform poorly (as was the case with some plots in 2000).• Technologies should be better adapted to farmer conditions. Participants in focus group discussions suggested that green manure species that mature within a shorter period and which can be inter-planted with crops and/or eaten would be preferred. Such technologies should be developed so that they can be broadcast in the farm, without necessarily having to be planted carefully in lines or rows. The main concern was that new technologies should not require rigorous skill and experience.• Group-based approaches, including collectively identified and run plots can be effective venues and tools for passing new technologies to farmers. In Emuhaya, several 'Farmer Field Schools' have emerged spontaneously to broaden community participation beyond the original, rather exclusive 'Adaptive Research Farmer Groups'. Local level meetings where farmers could exchange ideas have been tried in the past in other sites, but have not been sustained. It is necessary to involve many people in activities of dissemination. Awareness can be done through field days, demonstrations, visits or exposure tours to other areas.• It is widely felt that individualistic behaviour and the absence of 'traditional' practices that once united communities (beer brewing, labour sharing, etc.) undermine collective endeavours today. It is certainly true that few activities promote positive competition amongst farmers. Household differences and clan rivalries are also major sources of division, although most key informants felt that they could be overcome with good leadership.• Low interest in research work was partly attributed to poor leadership. Researchers, like local leaders were said to \"stand before farmers and address them\". The two were therefore similar. Just as local leaders never delivered on their promises, research was initially seen to be unproductive. For instance, a bean variety that is suitable for N Eastern Kenya was planted on one of the key informant's plot in Emuhaya. As with the poorly performing trial plots in 2000, this inadvertently created the impression that \"if a specialist's work failed, what is the point in learning how to copy it?\" • Farmers have 'tools' of measuring researchers. Those with meaningful intentions and hardworking are known and easily draw farmers' attention. Farmers should be consulted when deciding on ways of teaching.• Farmer research groups have limited participation of non-members through charging of subscriptions. Most farmers perceive subscription as extortion and expressed their objection that \"information from research bodies should not be passed through such groups\".To investigate the dynamics of how agro-ecological knowledge is generated and shared within a community, two master's level research activities are being conducted. The first project takes a more anthropological approach to understanding the role of local indicators of soil fertility change (particularly plant species and plant growth traits) and the degree to which different groups or individuals have come to recognise given indicators, or value the information that those indicators impart. The second study (still in preparation) will take a more ethnobotanical approach to understanding the distribution and relevance of indicator species, and will likely be situated in a contrasting environment.\"The role of indigenous knowledge in the management of soil fertility among smallholder farmers of Emuhaya division, Vihiga district.\"Justification: This study will add to the search for information on soil fertility management being pursued by many researchers and planners. Besides, there is a growing appreciation and recognition of the importance of local or indigenous knowledge in the sustainable use of natural resources. But the lack of information stands in the way of good understanding of these methods. By taking time and effort to document the systems, they become accessible to change agents and client groups (Brokensha et al. 1999: xv).The study does not, however, pretend that local knowledge and practices has the quick solution to the many problems facing farmers in the area of soil fertility management. Far from that, it recognizes the importance of integrated knowledge systems (modern and indigenous) and while focusing on the latter the study will pay attention to the former.The Folk Ecology Project (that provides a background for this study) needs specific information that can facilitate the integration of two knowledge systems (modern and indigenous), which eventually will enable scientific information to become a component of the larger pool of local knowledge to be more efficiently applied by the local people themselves particularly in the area of soil management.The Emuhaya division study site lies within a region, which has poor subsistence economy due to unreliable rainfall and highly fragile soils. Smallholder farmers in this region face the double tragedy of environmental degradation and increasing demand for food. While the extension workers and other agencies could be willing to assist, their efforts could be hampered by the prevailing low socio-economic status, especially among the small farmers. This, therefore, calls for the need to carry out a study, which could inform the donor community or, more importantly, the policy makers and communities themselves to enable them formulate a broad strategy within which resources can be more effectively focused.The findings of this study could, therefore, enable governments, policy-making bodies, nongovernmental organizations and donors to formulate and design strategies that can alleviate suffering emanating from soil nutrient depletion among smallholder farmers. Agricultural research institutions can also base on the findings to institute the intervention programmes that could improve the conditions of smallholder farmers so that they are not left vulnerable to adverse environmental effects. Extension workers can also use the report to enable them understand the indigenous knowledge perspective of soil fertility management practices.In addition, the findings are also potentially replicable. Brokensha et al (1999) argue that it is quite apparent that indigenous innovations, which are found to be effective in one part of the globe, can be equally effective when made available to populations in similar ecological conditions in other parts of the world. The documentation of the vast amount of unrecorded; often rapidly disappearing indigenous knowledge could provide the basis for many effective development interventions, if this knowledge could be shared. The general objective of the study is to describe indigenous knowledge of soils and how it relates to the management of soil fertility in the study area. Specific objectives include: i) To identity the local diagnostic criteria for differentiating soil types among smallholder farmers within the study area. ii)To identify local indicators for discerning soil nutrient depletion or loss among the study population. iii)To investigate the soil fertility management practices used by smallholder farmers in the study area.The field research phase of this study covered the long rains growing season of 2002, allowing the student to follow the on-farm activities and decision-making processes of key informants responding to various indicators of crop performance and soil fertility change. As such, it was expected to provide a useful window on an important aspect of local ecological knowledge and the extent to which it can (or does) inform local practice. Many of the older key informants, for example, have stressed that much of the knowledge they have acquired about changing agricultural conditions is no longer particularly relevant to their livelihoods for the simple reason that their land base is now so constrained that there are fewer opportunities to match crops to given micro-sites on farm. The adapted knowledge of younger farmers, however, indicates that local soil variability can still be profitably exploited with different management strategies, at least by some classes of motivated individuals.Somoni Franklin Mairura Kenyatta University, Kenya Rationale: Local plants as indicators of soil quality, like other biological indicators of soil quality, simultaneously reflect changes in the physical, chemical and biological characteristics of the soil. Because of their integrative nature they are often better early warning indicators than other conventional methods to detect changes in soil quality.Natural and agricultural systems respond in a similar way to degradation and regeneration processes through the ecological principle of succession. During succession, plants and soil organisms that are best adapted, gradually substitute those least adapted, because of the selection exerted by changes in soil characteristics (i.e. some plants can tolerate more degraded soils than others, etc.). If we are able to identify local plants used by farmers to characterize their soils across a region we may be able to organize this information and identify trends which can provide insights about their potential use in making decisions about land management.CIAT's work in Latin America has shown the important role played by local plants as indicators of soil quality (Barrios and Escobar, 1998). This document proposes a collaborative activity among CIAT, SWNM and AHI scientists to identify local plants used as indicators of soil quality in the Eastern Africa region using the AHI sites as a representative sample (i.e. Kenya, Tanzania, Uganda).This collaborative work will lead to the preparation of a table with local plants used as indicators of soil quality to be included as a contribution of AHI to Guide #1 \"Identifying and Classifying Local Indicators of Soil Quality (LISQ), Eastern Africa Edition ( 2001)\". Within this context, work will clearly identify the localities where the observations are being conducted, and will select key informants and elder representatives of the different farmer communities in the study area for group analysis (brainstorming) sessions. The following questions will guide the discussion for identifying and prioritizing local plants as indicators of soil quality from the local knowledge base: i) Are there any local plants (weeds, shrubs, trees) that only grow in fertile soils? ii)Are there any local plants (weeds, shrubs, trees) that only grow in poor soils? iii)Are there any local plants (weeds, shrubs, trees) that grow in all soils but that according to their growth, vigor and color can be used as indicator of the soil condition? iv)If you were buying a new plot which plants would you use to characterize the quality of such plot for agricultural purposes? v)After several seasons of cropping, you decide to leave your plot fallow by allowing natural regeneration of the native vegetation to take place. At what stage in that regeneration do you go back to cultivation? Are there any plants that indicate that your plot is ready for cultivation again? Information gathered will be organized and prioritized using pair-wise ranking in order to provide a list of most important to least important of all the plants used as indicators of soil quality.Evaluation of current ISFM options by participatory and formal economic methods JJ Ramisch and I Ekise TSBF-CIAT, PO Box 30677, Nairobi, Kenya Rationale: Declining soil fertility problem is the single greatest threat to food security and livelihoods in Western Kenya. Findings of most soil fertility research work in the region indicate that the soils of this region are generally deficient in Nitrogen and Phosphorus nutrients. This problem has been caused by high population density and poor farming methods. For instance in Emuhaya area, farmers continuously crop their fields with minimal use of inorganic or organic fertilizers. This type of farming can not be sustained in the long run and if not checked could lead to deterioration in the farming environment. Some of the indicators of a deteriorating environment are; sharp decline of crop harvests, high incidences of crop and animal pests and diseases, frequent famine, deteriorating farm incomes among others.Progress: A baseline survey of soil fertility management practices and socio-economic conditions was completed and analysed for 314 farmers in the West Kenya site. The methodology was shared with the Ugandan and Tanzanian sites. These data are being compiled and analysed along with comparable studies conducted at the other BMZ project sites in West Africa (Togo and Benin) to produce a scientific paper relating soil fertility management practices to the contrasting socio-economic and agro-ecological conditions of the sites.Farmers, extension, and KARI-Kakamega field staff were trained in participatory monitoring and evaluation methods. Several forms of farmer recording keeping were introduced in 2001 to monitor and evaluate progress with the soil fertility management technologies. However, lack of funds has limited follow-up, which has lead to widely varying levels of farmer interest and disparate standards of data collection.A good number of partners have since initiated trials in the region whose main goal was to enable farmers to produce agricultural products while reversing nutrient depletion on their soils. The purpose of this was to increase the farmer's capacity to develop, adapt and use integrated nutrient management strategies. The integrated soil fertility management options tried include; biomass transfer using Tithonia diversifolia, use of improved fallow plants (Mucuna, Crotolaria grahamiana, C. ochroleuca, C. paulina, Canavalia, Sesbania sesban etc), use of high quality compost, integration of inorganics and organics. The initial target number of farms was 60 located in 5 villages of Ebusiloli sub-location of Bunyore East location in Emuhaya division of Vihiga district. The work was implemented through the farmer research group framework, which focused the village as the unit of research work. Each village was organized into a research group with elected officials managing their respective groups. The specific objectives of this study are: i) To quantify the costs and benefits of the practiced ISFM technologies in order to show the profitability of each technology. ii)To conduct participatory ranking of the ISFM options based on farmers criteria and perceptions. iii)To identify the constraints facing the ISFM practitioners and possible solutions to overcome them in order to improve the adoption of technologies being practiced. iv)To build the capacity of the farmer field schools to innovate and share the results for collective action.The FFS Framework: The farmer field schools work together to implement the study. Suitable farms were identified and the owner contracted using the procedures of TSBF and ICRAF being currently used to implement other trials. The decision support systems (DSS) layout for the trial (see section 2.3) in Emuhaya was adopted. There is concern from the farmers that treatment plot sizes need to be increased for more visibility. They propose to have 10 m x 10 m plots. The farmer field schools propose to include the local (indigenous) plants and test them as well. The treatments will be randomly selected and established.Rationale: In sub-Saharan African countries like Kenya, small-scale farmers account for about 70% of the over all production and produce more than 75% of the total food crops. Soil erosion, depletion of ground cover due to overgrazing and nutrient depletion due to continuous cropping has lead to low living standards among the majority of the rural households. The depletion of the natural resources (land and forests) as a result of population pressure and continuous cropping in the study area does not augur well for the future generations who are expected to live on and derive their subsistence from such lands.To reverse the trend of rapid decline in the quality of soil and the physical environment, large investment in soil fertility technologies and soil conservation works is needed. This study seeks to justify and warrant such investment by providing quantitative and empirical evidence of the importance, appropriateness and economic competitiveness of agroforestry-based and other integrated soil fertility management (ISFM) technologies as strategies that are potentially capable of solving and alleviating productivity problems. The findings will be useful in terms of postulating suggestions to policy makers pertaining the incentives and institutions that can be put in place by the government and other stake holders to enhance the promotion and expansion of the emerging technologies of addressing soil nutrient depletion.Training and capacity building: Two master's level research projects are currently on-going. The first uses the policy analysis matrix (PAM) technique to evaluate the private and public benefits and costs of different ISFM options. This approach is particularly useful for examining the role of transaction costs and market failures in influencing profitability of new technologies. The second study determines whether the soil fertility management and livelihood enhancement needs of different classes of farmers are being met with the ISFM options currently available to them, by contrasting the profitability of different options (using gross margin analysis).The Competitiveness of Agroforestry-based and other Soil Fertility Enhancement Technologies for Smallholder Food Production in Western Kenya.Abstract: Most countries in sub-Saharan Africa have been faced with persistent food insecurity accompanied by low and declining agricultural production and productivity. Although in Kenya population growth has been on the decline, increased settlement on arable land has exerted pressure and heavy demands on natural resources especially land. As a consequence, continuous cropping has been very common among majority of the smallholder farmers leading to soil nutrient depletion. Many studies in Kenya have shown that soil fertility depletion among smallholder farms is responsible for the persistent food insecurity and declining per-capita food production.In order to address the soil fertility problem, researchers in International Centre for Research in Agroforestry (ICRAF) and Tropical Soil Biology and Fertility programme (TSBF) have been able to develop and promote agroforestry-based technologies. They include biomass transfer and improved fallows.Although these agro forestry based technologies together with Minjingu rock phosphate are being used by farmers in western Kenya, little is known about their economic competitiveness in terms of how efficient resources are being used to produce food under these soil fertility enhancement technologies. The proposed study is an attempt to bridge the above-mentioned gap in knowledge by providing a quantitative evidence of farm level profitability (both private and social) of food production under the above mentioned soil fertility replenishment technologies. The study will be carried out in Siaya and Vihiga districts, western Kenya.The Policy analysis methodology (PAM) will be used to analyse both primary and secondary data. A multi stage stratified sampling method will be used to select a total of one hundred and twenty farmers, sixty from each of the two districts. The selected farmers will be interviewed using structured questionnaires.A reconnaissance survey will be conducted in the area of study to identify the farmers to be interviewed. A questionnaire pre-test will be done on farmers in the area but the sample of the pre-test farmers will be outside the sampling frame.The over-all objective of the study will be to determine the competitiveness of both agro-forestry based soil enhancement technologies and use of Minjingu Rock Phosphates for smallholder food production. The specific objectives will include: i) To determine the financial profitability of food production under agroforestry-based technologies (improved fallows and biomass transfer) and Minjingu rock phosphate as alternative soil nutrient replenishment technologies. ii)To determine the social profitability of food production under agroforestry-based technologies and Minjingu rock phosphates as strategies of the soil fertility enhancement. iii)To compare the competitiveness of both inorganic fertilizers and agroforestry-based technologies for food production. iv)To compare the profitability of maize and horticultural production using agroforestry based technologies.There has been renewed attention on soil fertility replenishment in Sub-Saharan Africa as critical to the process of poverty alleviation, as symbolized clearly by the award of the 2002 World Food Prize to Pedro Sanchez, a pioneer in the field. Soil fertility is crucial because in Africa poverty is mainly a rural phenomenon. With 70% of the population in the rural areas and 60% of those living below the poverty line, a whopping 85% of the poor are found in rural areas (Mwabu and Thorbecke, 2001). Since over 95% of the rural population is engaged in agriculture to some degree, any short to medium term poverty reduction strategy that ignores agriculture is doomed to fail. In many places, the rural poor cannot expand land holdings. Per capita arable land in Sub-Saharan Africa has shrunk dramatically from .53 to .35 hectares between 1970from .53 to .35 hectares between and 2000from .53 to .35 hectares between (FAOSTAT, 2002)). Accelerated and sustainable agricultural intensification is required. Returns per unit land must increase in order to provide sufficient food for the (rural and urban) poor and output per worker must rise in order to lift the incomes of the poor. This has clearly not taken place as evidenced by the stagnant crop yields and per capita indices for agricultural and food production. For example, per capita agriculture, food, cereal, and livestock production indices are all below levels from 1990. While the first steps to reverse this trend are hotly debated, it is certain that increased agricultural productivity and improved rural livelihoods cannot occur without investment in soil fertility.There is no shortage of evidence showing the dismal state of Africa's soils. African soils exhibit a variety of constraints, among them: physical soil loss from erosion, nutrient deficiency, low organic matter, aluminum and iron toxicity, acidity, crusting, and moisture stress. Some of these constraints occur naturally in some tropical soils, but they are exacerbated by severe degradation processes. Degradation of some form is pervasive on the continent, with less than 20% of soils said to be unaffected by degradation (FAOSTAT, 2002) and about two-thirds of agricultural land to be degraded (Oldeman et al., 1991). About 85% of degradation is attributed to water and wind erosion, with the rest being mainly in situ chemical degradation (Oldeman et al., 1991).The lack of nutrient inputs among smallholder African farmers exacerbates the nutrient deficiency of soils. Fertilizer use was never high in Africa. Exchange rate devaluations and the termination of government fertilizer subsidy programs throughout the continent over the past fifteen years have sharply increased the real price of mineral fertilizers, putting them beyond the reach of most small farmers in Africa, at least at anything approaching recommended application levels. As a result, while the rest of the world averages 97 kilograms of fertilizer per hectare, in Africa, only 9 kilograms are applied to the average hectare of land (Gruhn et al, 2000). The rate is lowest in central Africa (2 kg per ha) and in the Sahel (5 kg per ha). Even when fertilizer is combined with other organic sources, studies throughout the continent have found high negative nutrient balances to occur in nearly all countries (Henao and Baanante, 2001). The estimated losses, due to erosion, leaching, and crop harvests are sometimes staggering, at over 60 -100 kg of N, P, and K per hectare each year in Western and Eastern Africa (e.g. Stoorvogel and Smaling, 1990;de Jager et al. 1998).Integrated soil fertility management (ISFM), developed more fully in section 3, is being widely studied and is rapidly becoming more accepted by development and extension programs in Sub-Saharan Africa, as well as, most importantly, by smallholder farmers in Sub-Saharan Africa. This paper begins with a brief setting of the context, demonstrating the key variations in agro-ecology, market opportunities, and farming systems in Africa and how these will condition the incentives for ISFM. The following section synthesizes evidence to date on the biological and financial impacts of organic nutrient practices and ISFM. Section 4 synthesizes available evidence on markets for organic nutrients, including supporting markets for seed, labor and credit. Section 5 provides a comprehensive summary of evidence on farmer investment in and management of organic nutrients and ISFM. Lastly, we conclude the paper with implications for research priorities, design and dissemination of ISFM, and policy reform.Sub-Saharan Africa is very heterogeneous in terms of soils, climate, agricultural potential, market access, and population density. These differences influence the types of organic nutrients that are technically feasible to produce, the types of crops that will benefit from such application, opportunity costs of land and labor, and cost of acquiring mineral fertilizers. In short, the incentives for producing and using specific types of nutrient inputs are highly variable across the continent.The physical and agroclimatic conditions in Sub-Saharan Africa are extremely diverse. Broad agro-ecological zones range from the semi-arid tropics, with around 400-800mm of rain per year, to humid highland regions that may average over 1,800mm of rain supporting two growing seasons. Soils are also quite distinct in texture, inherent soil physical, chemical, and biological health, potential for erosion and other forms of degradation. For example, crusting is a major problem in the semi-arid zone, aluminum toxicity in the humid lowlands, and erosion in the hilly highlands. In the more favorable zones, a wider range of organic based systems will be feasible, but they will also need to compete against a wider range of agricultural enterprises for land and labor. In the drier zones where growing plants becomes riskier and costlier, livestock assumes a more important role in the provision of organic nutrients.Soils are also highly varied within small geographic areas. They maybe affected by physical features such as topography or historical land use and vegetation cover. Thus, it is quite common to find relatively fertile soils where deposition has taken place due to erosion. Soil variation may also occur across or within farms due to management patterns. For example, greater soil fertility status has been found among wealthier than poorer households in western Kenya (Shepherd and Soule, 1998) and in plots near homesteads (Prudencio, 1993).Population densities vary noticeably within each of these zones, but generally, population pressure is highest in the more favorable agricultural zones. They are relatively lower in the semi-arid lands and minor portions of the humid lowlands (e.g. in forest margin areas) and subhumid zone (e.g. Zambia). They are highest in the highlands of East Africa, with densities of over 600/km 2 being very common. Densities of 250/km 2 or more are also found in some humid lowlands and sub-humid areas in West (e.g. Nigeria) and Southern Africa (e.g. Malawi). Such densities imply that average farmsizes among smallholder farmers will be 2 hectares or lower. Small farm sizes limit farmers' ability to find niches for the production of intermediate inputs for green manure or feed for livestock. Despite high population densities, the agricultural labor supply is not always plentiful in such areas, especially where school enrollment rates among children are high and non-farm income-earning opportunities are strong. Moreover, many very poor rural households are relatively labor scarce, exhibiting high shadow wage rates (Barrett and Clay forthcoming), limiting uptake of labor-using technologies, even among the poor in high population density areas.Market infrastructure development is similarly varied across the zones, but is not fully functional or efficient in any of the zones. There are low densities of main trunk roads with feeder roads that are of low quality and often seasonally impassible. The more densely populated areas enjoy somewhat better transportation opportunities, piggy-backing on public transport vehicles and greater densities of market centers. Despite a general tendency towards liberalization of both input and output markets throughout the continent, in some cases government parastatals still play an important role (e.g. coffee in Kenya, maize in Malawi). Further, liberalization has yielded spatially heterogeneous and generally mixed price and market access incentive effects due to changing risk characteristics, limited inter-seasonal credit availability, and meager private storage or transport capacity (Yanggen et al. 1998, Barrett and Carter 1999, Reardon et al. 1999).The Integrated Soil Fertility Management (ISFM) paradigm acknowledges the need for both organic and mineral inputs to sustain crop production without compromising on environmental issues (Buresh et al., 1997;Vanlauwe et al., 2002). The paradigm further acknowledges that plants also require a conducive physical, biological, and chemical environment, apart from nutrients, to grow optimally. Besides these organic and mineral inputs, the soil organic matter pool, which reflects past soil management strategies, is another substantial source of nutrients. Each of these sources contributes to crop production and the provision of environmental services individually, but more interestingly, these resources can be hypothesized to interact with each other and generate added benefits in terms of extra crop yield, improved soil fertility status, and/or reduced losses of nutrients to the environment.The earlier work on soil fertility management in SSA focused on the use of mineral inputs to sustain crop production. Numerous studies that have looked at crop responses to applied fertilizer report substantial increases in crop yield and financial returns (e.g. Yanggen et al., 1998;Snapp et al., 1999). National fertilizer recommendations exist for most countries, but actual application rates are nearly always much lower due to constraints of a socio-economic rather than a technical nature (see section 5). On the technical side, however, mineral inputs were further discredited due to the observed environmental degradation resulting from massive applications of fertilizers and pesticides in Asia and Latin America between the mid-1980's and early-1990's as a spin-off of the Green Revolution (Theng, 1991). As a result, soil fertility management strategies were refocused towards the use of organic amendments and considerable enthusiasm emerged around so-called \"agro-ecological\" approaches to agricultural development in the tropics (Uphoff 2001).Among the most commonly used or promising organically based soil nutrient practices are: animal manure, compost, incorporation of crop residues, natural fallowing, improved fallows, relay or intercropping, and biomass transfer. These are briefly described in table 1 below. While we focus on soil nutrient management practices, there are a host of other management practices that are vitally important to overall soil fertility, including soil conservation techniques, weed management practices, and cropping strategies themselves.Initially, organic resources were merely seen as sources of nutrients, mainly nitrogen (N), and a substantial amount of research was done on quantifying the availability N from organic resources as influenced by their resource quality and the physical environment (see Palm et al., 2001, for example). Various classes of organic resources were identified based on their short-term N supply, which in turn depends on nutrient acquisition methods, concentrations of nutrients in biomass, total biomass production, and decomposition characteristics (Vanlauwe and Sanginga, 1995;Vanlauwe et al., 1998). More recently, other contributions of organics have been emphasized in research, such as the provision of other macro and micro-nutrients, reduction of phosphorus sorption capacity, carbon/organic matter, reduction of soil borne pest and disease spectra in rotations, and improvement of soil moisture status. There are some key differences in the way that the organic systems contribute to soil fertility. Those systems that use nitrogen-fixing species are able to add nitrogen without withdrawing it from soils (either in situ or ex situ). Some can produce over 150 kg of nitrogen per hectare (e.g. a single season crotalaria fallow). Plant systems that are based on trees may further recycle deep nutrients (through roots) that would otherwise have been unavailable to annual crops. The different systems are not necessarily equally effective in providing nutrients. Organic sources will differ in terms of nutrient content, mineralization processes (in which the nutrients in the organic compounds can become available to the crop), and the provision of other soil fertility benefits (e.g. weed reduction). Aside from the organic source itself, management aspects can also affect the effectiveness of organics in increasing soil fertility. A key management distinction is the growing of legumes in situ (as opposed to transferring biomass from outside the plot) which can provide other benefits to crops through rotation affects (e.g. reducing the incidence of weed) and through water infiltration effects (from the root systems).Despite these positive aspects, organic nutrient systems are not able to sufficiently replenish soils by themselves. First, concentrations of phosphorus and potassium are very low in organic manures. Second, the efficiency with which N and other nutrients can be used by crops can be low. Other problems related to the sole use of organic inputs are low and/or imbalanced nutrient content, unfavorable biomass quality, limited land for production of organic material, or high labor demand for transporting bulky materials (Palm et al., 1997).It has been recently acknowledged that organic and mineral inputs cannot be substituted by one another and are both required for sustainable crop production (Buresh et al., 1997;Vanlauwe et al., 2002). This is due to (1) practical reasons -the amount of either fertilizer or organic resources alone would not be sufficient or organic resources were found unsuitable to alleviate certain constraints to crop growth, e.g., the lack of P in Nitisols with strong P sorption characteristics (Sanchez and Jama, 2002) and (2) the potential for added benefits created through positive interactions between organic and mineral inputs. Several attempts to quantify the size of added benefits and the mechanisms creating those have been made. Vanlauwe et al. (2002) reported that integration of maize stover increased the recovery of urea-N, most likely due to its temporary immobilization of urea-N. In a multilocational trial in West Africa, Vanlauwe et al. (2002) demonstrated added benefits from combined organic and mineral treatments through reduced moisture stress at critical growth phases of the crop. In a set of trials in sandy soils of Zimbabwe with various mixtures of cattle manure and ammonium nitrate, Nhamo (2001) observed added benefits ranging between 663 and 1188 kg maize grains per hectare. This synergy was attributed to the supply of cations contained in the manure.Although the above list of observed positive interactions between organic and mineral inputs is not exhaustive, very often these inputs are also demonstrated to have only additive effects. But because of declining marginal increases from one single type of input, the additive effects are often superior in terms of overall yields and net returns, as shown by Bationo et al. (1998) for millet in Niger and Rommelse (2000) on maize in Kenya. Fortunately, negative interactions are hardly ever observed, indicating that even without clearly under standing the mechanisms underlying positive interactions, applying organic resources in combination with mineral inputs has negligible downside risk and considerable upside potential, thereby constituting an appropriate fertility management principle.The ISFM paradigm has further broadened the scope for potential interventions in a number of ways. First, interactions between various crop growth factors were widened beyond nutrients. In Sahelian conditions, e.g., Zaongo et al., (1997) observed striking increases in water use efficiency for sorghum after application of fertilizer. Secondly, recognizing that soil fertility varies widely within a farm due to site-specific management by the farmer with drastic effects on crop yields, attempts are on-going to target resources, both of organic and mineral origin, to this within-farm variability in soil fertility status, rather than developing blanket recommendations. Bationo et al. (Unpublished data) showed a considerable improvement in P use efficiency from 47 to 79% when applying the P on a non-degraded homestead field rather than a degraded bush field. Vanlauwe et al. (Unpublished data) showed that N fertilizer use efficiency decreased from 45 to 30% when topsoil carbon contents increased from 0.3 to 0.8%. Thirdly, ISFM also highlights the need for improved germplasm. Improved crop germplasm does not only have a major role to play in improving nutrient acquisition but also in providing more organic inputs. Efforts have recently been made by various research centers to develop dual or multipurpose grain legume varieties (e.g. Sanginga et al., 2001).In summary, there is considerable evidence demonstrating the important contributions of organic matter to agricultural crop yields. There is more limited, but still significant evidence attesting to the positive impacts of integrating organic and mineral nutrient sources in the short and long term. One interesting caveat is that nearly all research on ISFM has taken place on cereal crops. Yet, as we shall see in section 5, much fertilizer use by smallholders in Africa is steered towards more high value crops. The effects of organics and ISFM on non-cereals remain under-researched.There are likewise several socio-economic sources of complementarity between organic and mineral inputs in soil fertility maintenance. Mineral fertilizer must be brought to the farm while organics can be home-grown, saving on transport costs and reducing uncertainties of market acquisition. The two approaches to soil fertility management require investment using different household resources, with fertilizer requiring financial capital and organics requiring labor and land (initial investment in livestock will require capital). The capital-intensive nature of fertilizer use is exacerbated by inflexible packaging arrangements creating a minimum $20 -$30 expenditure for a single bag. Several development projects and retailers sell fertilizer in smaller amounts, but farmers' lack of trust in shopkeepers seems to inhibit the growth of decentralized repackaging. Finally, in terms of quantities available, imported mineral fertilizers are in theory plentiful if the demand is there. On the other hand, production of organics is limited by available land and therefore supplying sufficient amounts for one's farm, let alone for sale in the market, can prove challenging.Macro or meso level factors may impinge on the ability of communities to access certain types of nutrients. For example, fertilizer has been absent from retailers in Uganda until recently and is more readily available in peri-urban areas than in remote areas, very few cattle keepers are found in Malawi, and many types of green manures cannot grow effectively in drier zones. But it is the heterogeneity among households more than variation between agroecological zones that explains most of the observed differentiation in the use of different soil fertility practices. Significant uptake of integrated organic and mineral practices for improving soil fertility has occurred throughout SSA, in the highlands of East Africa (Murithi, 1998;Gebremedhin and Swinton, 2002;Place et al., 2002a;Clay et al., 2002), the humid lowland zone (Tarawali et al., 2002), the sub-humid zone (Mekuria and Waddington, 2002;Kristjanson et al., 2002;Peters, 2002), and the semi-arid areas (Freeman and Coe, 2002;Shapiro and Sanders, 2002;Kelly et al., 2002). Studies also show significant payoffs from the integration of mineral and organic sources of nutrients across different ecozones (Place et al., 2002a;Kelly et al., 2002;Shapiro and Sanders, 2002;Freeman and Coe, 2002;Peters, 2002;Mekuria and Waddington, 2002).Several interrelated micro-level factors are at play in farmer input use patterns, including commercialization and access to land, labor, and capital. It is quite well documented that fertilizer use is strongly linked to commercialized production of cash crops (Kelly et al., 2002), ranging from parastatal run input-output supply programs to informal and opportunistic networks of peri-urban agriculture. There is some evidence to suggest in cash cropping systems organic inputs replace fertilizer when fertilizer supply becomes problematic (Bosma, et al., 1996;Mortimore, 1998) or that the availability of mineral fertilizers for use on cash crops facilitates a broadened use of organic materials on food crops (Raynaut, 1997).The relationship between commercialization and organic systems is also in general positive (Murithi, 1998;Kelly et al., 2002;Freeman and Coe, 2002), but there are obvious exceptions. Use of manure on cereal food crops is an old practice in the Sahel and southern Africa and continues today (Enyong et al, 1999;Williams, 1999;Ndlovu and Mugabe, 2002). Experimentation with new, plantbased, organic inputs often begins with their application on cereal crops, following traditional practices such as heaping or burning of familiar plant residues (Snapp et al., 1999). But animal manure is also commonly used on higher value commodities such as potato, coffee, and vegetables (Freeman and Coe, 2002;Shapiro and Sanders, 2002). And, as with manure, farmers have shifted promising innovations using new green organics systems (or integrations of organic and mineral fertilizers) onto higher value commodities such as vegetables (Place et al., 2002a). Furthermore, small farmers appear to consistently favor organics that serve as more than just a soil fertility amendment, offering food or animal feed that can be consumed or marketed as well, as with dual purpose legumes such as pigeon pea.This pattern underscores that the positive yield returns described in the previous section can make the use of organics remunerative even in semi-subsistence systems, including places where purchased fertilizers remain unattractive. This difference, the propensity for using organics to increase production in high-value systems and farmers' preference for dual-purpose varieties over those that serve as fertilityenhancing inputs only, highlight the value of cash liquidity in areas plagued by a dearth of inter-seasonal credit. Those who earn cash from crop sales, or can avoid spending cash on input purchases, can often afford to hire labor or to purchase food and thereby dedicate their labor to input production on their own farm instead of having to hire out their labor in order to earn wages. But when the labor demands of the low external input (LEI) technology are substantial, as in many biomass transfer systems or other LEI technologies, the foregone wage earnings can impede adoption among poorer farmers (Reardon et al. 1999, Moser andBarrett 2002).Land availability commonly constrains use of organic inputs produced on farm, like improved fallows (Place et al., 2002a). On the other hand it is not a major factor in biomass transfer systems, which are often focused on small plots of high value crops. The evidence on the effect of labor availability on adoption of organic inputs is mixed. While additional labor effort is often identified by farmers, they commonly find ways to reduce labor burdens to fit their needs through adaptation of extended technologies. For example, intercropping of pigeon pea with maize in Malawi saves labor (and land) compared to a sequential system (Waddington, 1999). Farmers in Western Kenya are also opting to use local plant species (such as Tithonia or Vernonia) identified as good nutrient sources as additions to existing composting systems, which use labor in small increments rather than as part of cut-and-carry systems which would demand major labor inputs at the time of crop planting (Misiko and Ramisch, unpublished data).A key motivation for the promotion of organic nutrient systems is that by requiring little capital, they might reach the poor better than commercially distributed fertilizer. This is critical, because many studies have found that the poor are unable to use mineral fertilizers and the consequences on soil fertility and farm incomes are enormous (Soule and Shepherd, 2000). This largely seems not to be true in the case of animal manure because incomes tend to be highly positively related to livestock ownership. Manure use therefore appears to increase with a household's wealth (Mekuria and Waddington, 2002). But poorer households are using agroforestry-based nutrient systems and compost in Western Kenya at the same proportion as wealthier ones (Place et al., 2002a). Moreover, participatory methods are involving the poor much more in technology design.Will the use of organics encourage greater use of mineral fertilizers? 1 A recent study of agroforestry improved fallow and biomass transfer systems in Western Kenya found that the systems were being used by 30 -45 percent of those households who were not using fertilizer or manure (Place et al., 2002c). However, the use of agroforestry has not yet spurred an increase in the use of fertilizer. On the other hand, Abdoulaye and Lowenberg-DeBoer (2000) analyze data from Niger to show that patterns of intensification exhibit a pattern of graduation from manure to mineral fertilizer use. Expansion of options is good for smallholders. However, there remain information gaps as to how much the different options are being perceived as complements or substitutes by farmers.Markets depend fundamentally on there being positive net returns to moving goods across space or time, through transport and storage, respectively. The development of markets (formal or informal) for organic inputs in Africa, as throughout the world, has been shaped and constrained by the extreme variability in the supply of organic resources and their relative 'bulkiness' (low nutrient value per unit mass). These factors conspire to limit trade in organic inputs, leading to extremely localised patterns of use.The supply of organic resources that are potentially important contributors to agriculturemanure, crop residues, and other plant biomass -is both seasonally and spatially variable. Spatial variability can be observed as gradients of input use at the farm scale, inter-household variability based on differential resource endowments, and variability at the landscape and higher levels due to agro-climatic differences. Seasonal variability affects the abundance of key materials: crop residues are available in vast quantities only at harvest or as thinnings before then, manure is more abundant during rainy seasons than in dry ones but more likely to be dispersed by grazing across the landscape. Temporal variability is also seen in the quality of materials. The nitrogen content, in particular, of manure or harvested organic materials declines rapidly with the passage of time, as does the overall nutrient value of young plant materials like leaves if they are allowed to mature or senesce. Inter-seasonal storage of organic soil nutrient amendments is therefore impractical.The second factor, bulkiness, is a key constraint on the transport of organic materials over any significant distance for use as inputs. The much observed 'ring management' of many Sahelian farming systems (cf. Prudencio, 1993;Ruthenberg, 1980) results from the concentration of inputs on fields declining with increasing distance from their source (typically the homestead). Comparing yield benefits from manure application against the labor involved in transporting it, Schleich (1986) found that for a community in Côte d'Ivoire, ox carts were profitable up to a distance of 1 km, whereas transport on foot was not profitable at any distance. Since animal powered transport can increase the efficiency of laborintensive transport activities to the point of profitability, dynamic community-level markets for the exchange of draft power have been reported for transporting manure (Mazzucato and Niemeijer, 2001 in Burkina Faso;Ramisch, 1999 in Mali;Tiffen et al., 1994 in Kenya;Sumberg and Gilbert, 1992 in the Gambia).Because transportation is an important limit, there is a strong incentive to produce organic inputs in situ (such as companion planting of legumes with cereal crops or as improved fallows in rotation with them). An animal based analogue is the corralling of animals on fields in the dry season, which exchanges crop residues for manure. Throughout much of West Africa, the manure of large semisedentary and transhumant herds is a key resource for settled farmers (Landais and Lhoste, 1990;Bonnet, 1988;McIntire and Gryseels, 1987;Powell and Coulibaly, 1995), and such manure is often the catalyst for inserting pastoralists into the exchange networks of a settled community (Ramisch, 1999;Guillard, 1993;Dugué, 1987;Lachaux, 1982). Where markets can valorise increased crop production, exchanges of 'surplus' manure or compost between settled farmers are also common, either for cash (Tiffen et al., 1994) or labor for other activities (Ramisch, 1999;Guillard, 1993).High labor requirements for collection, transportation, and application of organic inputs are an important limiting factor for market participation. Where local labor markets and credit are incomplete, a household's capacity to use organic inputs depends primarily on the availability of household or reciprocal labor (Ahmed et. al, 1997;Barrett et. al., 2002). This has implications for labor allocation decisions that may not be consistent with households' income diversification strategies. The low quality of manure being used by many farmers under traditional management systems results in low concentration of plant nutrients (especially nitrogen and phosphorous) and correspondingly low returns to farm labor (Probert, n.d.). Under these circumstances households may seek to free farm labor to pursue off-farm activities that provide a higher return or are less risky. HIV/AIDS has also reduced labor availability for farm work in many countries in eastern and southern Africa These households are likely to prioritise labor saving technologies even in perceived labor surplus areas. Nonetheless, surprisingly little is known about how returns to integrated soil nutrient management practices compare with alternative investments off-farm.The problem lies not just in markets for soil amendments themselves, but also in the materials for in situ production of organic inputs. Farmer willingness to pay for germplasm for green manure is low because of free distributions by projects, high quantities demanded, and an ability to harvest and reuse seed for most green manure plants. Where intensification of leguminous grains is linked to market development farmers have shown greater willingness to invest in improved seeds (Jones et. al., 2002). The challenge here lies in identifying the right varieties that have the best potential for fixing nitrogen while at the same time meeting preferred market requirements for the food product beyond soil fertility improvement. The proliferation of markets for Mucuna seeds in West Africa, for example, was related to its perceived ability to suppress the noxious grass Imperata. Within 2-3 years this weed was controlled and Mucuna no longer marketed (Houndekon et al., 1998). Species with multiple benefits, such as dualpurpose soybeans or cowpeas are more likely to be adopted than those purely for soil improvement. Social networks play an important role in facilitating the reallocation of slack resources (Mazzucato and Niemeijer, 2001). The anecdotal evidence that exists for the development of seed distribution markets for legume cover crops suggests that social networks are paramount in spreading both information and the small amounts of seed that become periodically available for members outside the group (Misiko, 2000).Integrated soil fertility management practices are thriving in agricultural research and development projects, as the use of organic inputs increases, both on a stand-alone basis and in conjunction with mineral fertilizers. Much of this initiative is due to farmer innovation and adaptation, often in response to macroeconomic and sectoral reforms that have driven up real fertilizer prices throughout the continent. Organic systems have been found to complement fertilizers in many ways, both in a biophysical sense (enhancing soil fertility beyond nutrients alone) and in a socio-economic sense (requiring different types of household resources). Some organic systems are performing well on their own and in integrated systems, as measured by yields and profits. Like mineral fertilizer, there appears to be more interest in, and impact from, the use of organics and integrated systems on higher value crops. Because of their low cash requirements, some organic-based systems are reaching poorer households that otherwise are scarcely using any fertilizer. But there are limits to the amounts of organics that can be produced on-farm, particularly where labor constraints bind. There remains insufficient evidence as to whether increased use or organic inputs is spurring increased overall use of nutrient inputs. While biophysical research in integrated soil fertility management is progressing rapidly, more research is needed on farmers' practices, including their innovations and integration of individual components. There is also an urgent need to extend both bodies of research to higher value crops and whole farm analyses.Markets for organic biomass are limited mainly due to the inherent characteristic of relatively low quality of nutrients per weight resulting in bulkiness. Markets have developed for animal manure, especially quasi-contractual arrangements between owners of free grazing cattle and stover owners. Markets for green manure do not exist to any significant degree. Markets for green manure germplasm have developed in response to demand from projects and from farmers when the plant yields feed or food product in addition to soil nutrient replenishment.In order to ultimately contribute to increased productivity through improved soil fertility management, a few steps can be highlighted. First, there is still need to develop more attractive options, components and integrated strategies for small farmers of which improved germplasm is an integral part. This requires tighter linkage and feedback between strategic and adaptive research activities. Farmers are moving quickly in experimentation and the researcher community must be more active in monitoring this work. This will require more partnerships among farmers, extension, development projects, and researchers to bring wider development efforts into the knowledge base of researcher.Second, because ISFM practices are knowledge intensive, a major challenge is to identify scaling up processes that are both effective and not too costly in terms of information provision and technical support. It will be especially challenging to overcome the many bottlenecks of information flow across different organizations, from organizations to communities, between communities, and between farmers within communities. There is a need to develop incentive systems that reward improved flows of information. Rewards to communities for their efforts similar to the Landcare system in the Philippines or the Presidential award in Kenya are worth exploring, as are ways of utilizing existing rural collective action (e.g. community-based organizations) to facilitate information flow.Third, there must be major efforts to make agricultural commercialization more attractive to small farmers. Low rates of market participation are leading correlates of both poverty and the absence of sustainable agricultural intensification through increased investment in the land (Barrett andCarter 1999, Reardon et al. 1999). Increasing commercialization requires improving access to input markets, including for working capital (e.g., credit, savings) needed to purchase mineral fertilizer, organic inputs and seed and to hire labor, perhaps especially for women, who are key soil fertility managers in much of the continent. This is relatively easier in favorable agricultural zones where investment in market infrastructure can have a big impact. Indeed private, commercial interests sometimes undertake such investment voluntarily in support of lucrative contract farming schemes. Stimulating greater market participation is trickier in drier areas, although research from South Asia suggests that the marginal returns, in terms of both poverty reduction and production value, are highest for road infrastructure investments in low potential rainfed areas (Hazell and Fan 2001). Roads are important, but the organization of marketing and finance demand attention as well, building on local self-help groups to help resolve coordination and contract enforcement problems bedeviling much commerce in rural Africa today.Rapid growth in experimentation with organic soil inputs has fuelled the emergence of an extremely promising integrated soil fertility management paradigm that is just beginning to be evaluated carefully. A wide variety of studies report widespread experimentation with ISFM across all agroecological zones in Africa, including by many farmers who had not been using mineral fertilizers. Nonetheless, problems of market access, and household-level availability of land, labor and working capital continue to limit the extent of adoption of ISFM among poorer small farmers. One finds pockets of active and effective users surrounded by vast areas of non-use. Much remains to be done, both in terms of research and development practice, to establish how best to employ the emergent ISFM paradigm to overcome or increase Africa's miniscule rates of mineral fertilizer application and stimulate agricultural productivity growth. The task is made all the more pressing by economic policy reforms that have caused a sharp drop in fertilizer use by small farmers in many areas. The core challenges to scaling up limited successes with ISFM to date appear threefold: improving integration between strategic and adaptive research, accelerating and expanding the flow of information among farmers, and increasing agricultural commercialization through improved market access, especially in lower potential rainfed regions.Abdoulaye, T., Lowenberg-DeBoer, J., 2000. Intensification of Sahelian farming systems: evidence from Niger. Agricultural Systems 64, 67-81. Ahmed, M., Rohrbach, D.D., Gono, L.T., Mazanghara, E.P., Mugwira, L., Masendeke, D.D. Alibaba, S., 1997. Soil The spread of solid and liquid excrement from animals, mainly cattle. Intensified livestock production systems involve the collection of manure in stalls or pens, while the more extensive systems involve direct deposition of manure by grazing animals.The collection and distribution of a range of organic compounds that may include soil, animal waste, plant material, food waste, and even doses of mineral fertilizers. Prior to application of compost onto the field, there is a period of incubation to decompose materials.The in situ cutting, chopping, and incorporation of crop residues into the soil. This operation is often done at the time of land preparation for the following season.Withdrawal of land preparation or cultivation for a period of time to permit natural vegetation to grow on the plot. The breaking of the crop cycle and lead to regeneration and the fallows can also recycle nutrients.The purposeful planting of a woody or herbaceous plant to grow on a plot for a period of time. In addition to benefits of natural fallows, improved fallows can achieve equal impacts of natural fallows in shorter time periods because of purposeful selection of plants, such as those that fix atmospheric nitrogen.Nutrient sources are integrated with crops in both time and space. The organic source may be a permanent feature on the plot such as with alley farming or scattered trees or may also be annual legumes. Intercrops are normally carefully planted, but trees in certain parkland systems (e.g. Faidherbia albida) are naturally growing.Relay systems are similar in sharing space with the crop, but the organic source is planted at a different time than the crop.The transport and application of green organic material from its ex situ site to the cropping area. The organic source may be purposefully grown or growing naturally.Finding common ground for social and natural science in an interdisciplinary research organisation -the TSBF experience J.J. Ramisch (TSBF-CIAT), M.T. Misiko (TSBF-CIAT), S.E. Carter (IDRC, Canada) Abstract: Continuing dialogue between the natural and social sciences means that the conception of \"development\", and of integrated natural resource management (INRM) in particular, continues a healthy evolution from largely discipline-based approaches to more integrative, holistic ones. Reflecting a microcosm of this evolution, the Tropical Soil Biology and Fertility (TSBF) Institute of CIAT is today dedicated to integrated soil fertility management and the empowerment of farmers through participatory technology development. Yet its origin in 1984 was as a body devoted to researching the role of soil biology in maintaining soil fertility, to combat declining per capita food production and environmental degradation. This paper examines the changing theoretical and methodological approaches of integrating social science into TSBF's research activities over the past decade, and identifies strategic lessons relevant to INRM research. The interdisciplinary \"experiment\" of TSBF has steadily taken shape as a shared language of understanding integrated soil fertility management. While individual disciplines still retain preferred modes of conducting fieldwork (i.e.: participant observation and community-based learning for \"social\" research, replicated trial plots for the \"biological\" research) a more \"balanced\" integration of these modes is evolving around activities of mutual interest and importance, such as those relating to decision support for farmers using organic resources. Since TSBF is working constantly through partnerships with national research and extension services, it has an important role in stimulating the growth of common bodies of knowledge and practice at the interface between research, extension, and farming. To do so requires strong champions for interdisciplinary, collaborative learning from both natural and social science backgrounds, the commitment of time and resources, and patience.As part of a CG-wide review, papers were invited which analyze the contribution of social research (sociology, anthropology, geography, political science, psychology) to research process, results, outcomes and development impact of agricultural or natural resource management research. Papers should assess the extent to which social research has influenced the relevance of research outcomes for the poor. Preference will be given to papers that analyze experience over time with social research and its impact in an organization such as a CGIAR Center, National Agricultural Research Institute or NGO, as distinct from a project. Topics to address include:1. Introduction: brief overview of the history of social research in the institution that identifies main phases and trends over time in number and type of staff and resources involved and their main objectives.processes : e.g. on problem identification, research priorities, client or target group identification, methodologies, on-farm approaches, technology design, criteria for successful results, scaling up, evaluation, impact assessment etc. How and why has this changed in relation to the main phases and trends in use of social research over time identified in (1) above ? 3. How has social research done by the institution been incorporated into the organization, its work culture, team composition, policies and procedures. Have synergies been achieved by combining social research with other disciplines? Why or why not? 4. Are there any effects of social research on results achieved by the institution, on adoption and use of its research results by client groups, outcomes of use for clients, and development impact associated with the institution's research. If there is no evidence to enable you to address this question, why is this ?The Tropical Soil Biology and Fertility (TSBF) Programme (now Institute) was created in 1984 under the patronage of the Man and Biosphere programme of UNESCO and recently incorporated into the Future Harvest system of food and environment research centres as a research Institute of the Centro Internacional de Agricultura Tropical (CIAT). As an international research body, the underlying justification of TSBF's work has been that \"the fertility of tropical soils is controlled by biological processes and can be managed by the manipulation of these processes\" (Woomer and Swift, 1994).Being an organisation with an explicitly biological and ecological mandate and origin, TSBF has nonetheless sought social science input into its research program since 1992. It has always been a small team (never more than six internationally recruited scientists) and therefore much of TSBF's considerable output has been generated through collaboration with partner organisations (both national and international), with special focus on sub-Saharan Africa. The decision to develop and maintain a core competency at the interface of social and natural sciences at TSBF since 1992, rather than looking for such competency from partners, is therefore significant. A decade after the creation of the Resource Integration Officer (since renamed Social Science Officer) position, it is worth re-evaluating the effects and effectiveness of this decision.This paper examines TSBF's historical record as a \"laboratory\" for developing meaningful interdisciplinary dialogue and collaboration, and asks whether what has emerged has been \"social soil science\" or merely \"soiled social science\". To illustrate some of the tensions inherent in interdisciplinary undertakings, examples of theoretical and methodological evolution are drawn from \"grey\" project literature, personal commentary, and publications. The strategic lessons learned from this particular organisation reflect in microcosm the much broader debates about the potential for \"rigorous\" science under competing disciplinary approaches to integrated natural resource management (INRM). They also address the assumption that developing a common institutional culture and language within INRM falls more to social scientist \"newcomers\" than to biological or natural scientists.The smallness of TSBF when contrasted with the larger international research centres has obliged an inherent recognition that the organisation cannot do all things in all places. As a result, strategic decisions about which research themes and methods to pursue become all the more important. \"Smallness\" has also meant that individual personalities and disciplinary backgrounds have had a much more direct impact on the organisational research agenda and that agendas can change with less institutional inertia than would be the case in a larger centre. On the down side, the institution has been very vulnerable to changes in core personnel (especially the gaps that occur when posts are changing hands) and frequently science has taken a back seat to mere matters of survival.The development of a TSBF research agenda that looked beyond the soil to the people cultivating it has moved from descriptive, characterisations of farming systems to more strategic study of social differentiation, power, and networks as they relate to soil fertility management innovation. An interest in dissemination has broadened into investigation of social dynamics, knowledge, and farm-level decisionmaking. There has also been a tradition of self-reflection, examining the consistency and coherence of TSBF's stated goals, methods, and actual practice, as well as the extent to which grassroots action conforms to its depiction to outsiders. As such, social science practice has developed quite healthily over the ten years 1992-2002, driven significantly by the following factors:a) The disciplinary background of the Social Science Officer (and to a lesser extent, that of field staff). Three people have held this position -Simon Carter (1992-1997, Geographer), Patrick Sikana (1998-2000, Anthropologist), Joshua Ramisch (2001-present, Human Ecologist) -and each has had preferred research topics and interests. In addition, Eve Crowley (1994-1996, Anthropologist) worked with TSBF on a Rockefeller Social Sciences Fellowship; a position shared half time with ICRAF. b) The demand for \"socio-economic\" understanding of processes being studied by other TSBF staff and collaborators. c) The natural evolution of projects from inception to later stages. This organic growth has typically moved from characterisation using very descriptive studies to more explanatory work building on existing practices through to development of longer-term interactive learning activities. d) Evolving social science debates concerning knowledge, power, and participation. The cosupervision of MSc and MA students from local universities has been an especially useful vehicle for maintaining contact with these debates. e) Responding to donor agendas, including but not limited to perceived needs for research results readily useful to farmers, a clearer understanding of agrarian change and its links to changes in soil fertility, livelihoods analysis, impact assessment, and identifying the most effective ways of \"scaling up\" organisational successes.There has always been a tension between the research agendas demanded from within TSBF by social scientists (i.e.: disciplinary interests, evolving projects and debates) and those expected from outside (i.e.: from other TSBF staff, partners, donors). This tension results from different research paradigms and differing ideas about the role of research in relation to social change. From the natural science perspective, the key contribution of social science to INRM often appears to be identifying and understanding the social factors that limit \"adoption\" or the \"appropriateness\" of given technologies. Other socio-cultural phenomena, such as \"policy\" might be acknowledged as important to the fate of different innovations, but most teams (even multi-disciplinary ones) lack the capacity to generate relevant policy-related questions, experiments or interventions. In other words, when the organisation is researching natural resource problems, the natural-social science dialogue has most often begun with identifying \"black boxes\" of external, social forces that need illumination, rather than defining truly interdisciplinary questions about how research (including technical research) can support positive change in rural societies.This tension is reflected clearest in the history of the social science position itself, which is discussed at length in the next section. Created in 1992, the post was originally charged with \"Resource Integration\". This step was perceived as a natural evolution for TSBF, which always held an ecological, systems-oriented approach to thinking. Although TSBF's strength remained at the plot level, the diversity of forces impinging on the plot draws attention naturally towards a hierarchical systemic analysis (Scholes et al., 1994).The Resource Integration Officer was therefore initially charged with \"developing a model for integrating biophysical and socio-economic determinants of soil fertility for small-scale farms\" (Swift et al., 1994). Under this rubric, social factors were expected to be integrated into holistic models as additional explanatory variables. Once key, perhaps universal variables were identified, these could then be added to a \"minimum set\" of characterisation data collected for TSBF sites (cf. Anderson and Ingram, 1993). However, the main contributions to the TSBF programme remained in terms of site selection, selection of themes for process research, and client group selection, with much less emphasis on experimentation, or monitoring and evaluation (Crowley, 1995).The Resource Integration Officer was therefore initially charged with \"developing a model for integrating biophysical and socio-economic determinants of soil fertility for small-scale farms\" (Swift et al., 1994). Under this rubric, social factors were expected to be integrated into holistic models as additional explanatory variables. Once key, perhaps universal variables were identified, these could then be added to a \"minimum set\" of characterisation data collected for TSBF sites (Anderson and Ingram, 1993). The main contributions to the TSBF programme were expected to centre on site selection, selection of themes for process research, and client group selection, with much less emphasis on experimentation, or monitoring and evaluation (cf. Crowley, 1995).2.2. Historical evolution 2.2.1. Carter (1992Carter ( -1997) ) The first incumbent in this post was a geographer, Simon Carter, with a background in both social and natural scientific traditions. Recognising the need to start from where TSBF \"was at\", but also charged with the task of helping to make the program's research more relevant to farmers, he began to work at the intersection between these two positions, and to generate information about how soil fertility was managed. Everyone in the programme agreed that it was important to know more about resource availability and use, and to begin to think about the relationships between research on soil fertility management and a better understanding of social and environmental change within African farming systems.Understanding spatial variability in soil management was also a common concern to the programme, with practical implications at two different scales. Understanding the importance of spatial variability at plot and landscape scales, and how farmers dealt with these, had clear practical implications for research on-farm and in communities, such as the questions research should address, involving farmers, and designing experiments. Secondly, given that a high priority for TSBF was the development of its African network (AFNET), identifying key regional differences in soil fertility management strategies could be key to developing AFNET. As a result a range of work was undertaken including development of simple GIS databases for East Africa, a more detailed one for Western Kenya, detailed formal survey work in Western Kenya, participatory characterisation of farmers' recognition and management of farm and landscape-level management of soil variability in Kenya and Zimbabwe.Carter (from 1994 with support from Eve Crowley) sought to align research at TSBF with ongoing debates on agrarian change in order to broaden the conceptual base underpinning many of the assumptions about the potential contributions of ecological research on soil fertility management to rural development in Eastern and Southern Africa. Hypotheses generated from the literature drove much of the data collection efforts undertaken from 1993-1996(eg. Crowley and Carter, 2000). In addition, efforts were made to expose AFNET members in Kenya and Zimbabwe to a range of on-farm research methodologies and tools and approaches that could, over time, facilitate inter-disciplinary exchange (Carter et al, 1992;Crowley & Carter, 1996). This work culminated in a four-country project funded by the EU from 1995-1998 (Carter & Riley, 1998).The modus operandi that evolved within TSBF between 1992-1996 had important methodological implications, however, for the research undertaken by the Resource Integration Officer, and later the Social Science Fellow. The pressure was on them to demonstrate, through quantitative means, the validity of social science perspectives and the fallacies underlying some of the assumptions of colleagues, as well as to collect quantitative data that would be of use for biological and economic modelling (little use was made of the additional quantitative survey data that was collected on behalf of other colleagues, who were simply too busy with other projects and priorities to explore the data). With hindsight it was probably a strategic error to agree to conduct an extensive formal survey in Western Kenya. A lot of time and labour was spent generating, collating, cleaning and exploring the data, and insufficient priority was given to interdisciplinary analysis, writing-up and dissemination of the results. Difficulties in collaboration, the departure of the Social Sciences Fellow, and increasing demands from other projects undermined the considerable investment the programme had made in this work, although the long-term worth of the dataset is undoubtedly high. On a more basic note, insufficient priority was given in the early years to simply learning to communicate more effectively across disciplines.Recognising that the unique contribution of the Resource Integration Officer to TSBF's overall research strategy was its attention to social questions, the position was renamed Social Science Officer in 1997. By this time it was recognised that the contribution of the post had had moved beyond collection of an enlarged \"minimum set\" and creating a more open \"sequence\" or menu of methods that would be useful for \"defining the resource bases and management strategies of different socio-economic groups\" (Carter and Crowley, 1995). Changes in personnel during 1996-7 had opened new opportunities for collaboration, and work moved into its strongest experimental phase, including researcher-managed experiments in conjunction with ICRAF, researcher-managed work under the EU project, and farmer participatory research begun in 1996. In 1997 a project was developed to support some of this on-farm experimental work. Significantly, it also included support for two masters students to look at how farmers in Western Kenya gained access to and shared knowledge about soil fertility management. This small step paved the way for a significant shift in focus over the next few years.2.2.2. Sikana (1998Sikana ( -2000) ) The brief tenure of Patrick Sikana brought the newly renamed Social Science Officer position towards much more autonomy on purely \"social\" research topics than had previously been the case. As a social anthropologist with farming system research experience in southern Africa, he prioritised deepening TSBF's understanding of farmers' local soil ecological knowledge and the rationales behind their existing soil management practices. This period also initiated critical investigations of how social networks aid and hinder the functioning of integrated soil fertility management (ISFM) research projects and the dissemination of ISFM knowledge. However, there was a significant lag time of nine months between Carter's departure and Sikana's arrival, which would have implications on the ground (discussed in methodological changes below). Furthermore, Sikana had been in the post slightly over a year, and just begun organising new projects for the Social Science Office when he was killed in the crash of a Kenya Airways flight from Abidjan in January 2000. His death devastated the small organisation at a time when its future was also being shaken by financial uncertainty.Indeed, the issue of continuity of personnel has had major impacts on developing an interdisciplinary and social science research agenda, at least in the short to medium term. Not only has TSBF seen significant turnover of personnel since 1992, but so has AFNET. The retrenchment of public sector employees, as part of structural adjustment or other \"reform\" programmes, has gutted national research bodies and extension services. The relatively low numbers of social scientists present in national systems must also been see in the light of the stark fact that they tend to be much more attractive to donors and thus more likely to move on from low paid national positions. Social scientists trained in participatory methods are also much less likely to return to agricultural research jobs when conservation and health present more prominent and well-funded fields. Finally, staff turnover in African organisations has been exacerbated by sudden deaths like Patrick's, attributable to disease, accidents, and general insecurity.The AfNet membership is still overwhelmingly natural scientists (over 150 soil scientists, biologists, agronomists) with social science represented only by six (socio-) economists. While there is a general appreciation that \"social science\" is important to the network, there is still great unfamiliarity with what can really be offered or understood. The emphasis remains on economic information about the \"profitability\" or \"adoptability\" of known technologies, with no expertise or experience in applying strategic, interdisciplinary research questions at the interface of human-environment interactions to soil fertility management. AFNET could have made it a higher priority to try to attract more social scientists, but soil and agricultural scientists need to be trained to recognise where social science can make their lives easier. This has to happen at university and in special training courses, and (rather like gender mainstreaming) has to have the soil and agricultural scientists in TSBF as its champions, not just the social scientists. Host institutions have also to provide the space for scientists to engage in interdisciplinary research. Unfortunately, while recognised by the various AfNet coordinators, this has tended to be subsumed, and therefore obscured, within the larger problem (true within AFNET as within the CG system more generally) of declining numbers of soil scientists faced with increasing obligations and expectations.The lack of \"champions\" for social science research within TSBF can also be seen in the example of Ritu Verma, an IDRC-funded MA student who worked with TSBF in Western Kenya from October 1997 to April 1998. Her research comprehensively examined gender and agricultural practice but without a strong link to the core of TSBF was never meaningfully integrated into other projects. Ironically, her book \"Gender, Land, and Livelihoods in East Africa: Through Farmers' Eyes\" (Verma, 2001) is the most extensive TSBF text produced by social science research but presents its arguments in such detail that it has been difficult to absorb or disseminate, making it a testimony to missed opportunities. Ramisch (2001-present) One of the main objectives since the arrival of Joshua Ramisch in early 2001 has been enhancing the \"institutionalisation\" of the social science research agenda. This search for greater continuity within the research agenda has been assisted by the recruitment of two full time research assistants (a socioeconomist based in Maseno, and anthropologist based in Nairobi), as well as broadened efforts at building a social science \"constituency\" within AfNet. Core activities of the office have retained an anthropological focus, including research on indigenous soil ecological knowledge, farmer decisionmaking, understanding innovation processes, and the role of social differentiation in ISFM practicesStaff turnover in 2001 also gave TSBF a chance at a relatively clean slate. The AFNET coordinator position was filled by Andre Bationo and Bernard Vanlauwe became the ISFM Officer at roughly the same time as Ramisch arrived. While there has been a risk of losing institutional memory through this process, the simultaneous arrival of so many new staff has facilitated team building, new collaborative activities, and presented opportunities for cross-disciplinary learning. Evidence of this interdisciplinary thinking has emerged clearly in presentations and papers written by core TSBF staff (e.g. Bellagio, Centres Week, andINRM presentations 2001, 2002;unpublished), as well as increasing numbers of interdisciplinary activities on the ground in Kenya, Uganda, and Zimbabwe.The strategic alliance in 2001 with the Centro Internacional de Agricultura Tropical (CIAT) has helped give TSBF greater financial security and a higher profile. It also presents opportunities to link the Institute to a broader interdisciplinary community and to draw on the expertise of CIAT's longestablished social research programmes. While the transaction costs of inter-continental collaboration are high, crosscutting endeavours within TSBF-CIAT have taken place around the Bellagio meeting in 2002, the training of an Argentinean in NUTMON methods in Ethiopia, and joint supervision of an M.Sc. student in Western Kenya.However, the 8 th AFNET meeting held in Arusha in May 2001, also clearly demonstrated that amongst partners TSBF is still perceived essentially as a biology-based organisation with minimal social science input. Active recruiting of social scientists has begun through networking and proposal development, but has been complicated by the rapid expansion of AFNET in the past two years. The massive influx of new members and the expansion of activity into West Africa have simultaneously increased the potential demand for INRM input and diluted the few interdisciplinary voices present within the network. The AFNET mandate of increasing the use of \"integrated\" approaches frequently takes a back seat to its more \"traditional\" and familiar mandate of increasing support of biological approaches to partner institutes through curriculum development and networked experiments. The role of social science within AFNET remains an unresolved problem, acknowledged as important (for \"integrated\" resource management, for greater \"adoption\", and ultimately donor approval of soil fertility management topics (Bationo, forthcoming)) but not backed by resources or strong champions within the network.A final point to note is that all of the social scientists who have worked at TSBF have been relatively young and in the early stages of their careers, whereas the biological scientists have generally been more senior. The onus has been on the social scientists to communicate novel ideas in terms their colleagues could understand or accept; this was relatively easy with concepts such as spatial variability, but much harder with feminist political ecology. Furthermore, in the past, strong personalities or opinions have tended to block communication between individuals and to limit interactions within the team. The new team that came together in early 2001 has begun to overcome some of these historical difficulties, further stimulated by meetings held in conjunction with the union with CIAT and the formation of the strategic Alliance for ISFM between CIAT, TSBF, and ICRAF. However, without a more senior social scientist or generalist present to mentor or to mediate communication, interdisciplinarity will always be a challenge.The most fundamental evolution has been from largely descriptive, empirical work towards developing more theory-driven, strategic research and the broader use of participatory approaches. At the same time, there has been a search for the optimal degrees of participation relating to the \"fieldwork\" aspects -which actors, doing which tasks, using which methods. This search has highlighted some of the still extant divides between the rhetoric of research aims and the realities of operational daily practice, as well as tensions that exist between different models of the role of research in stimulating change. Examples are drawn from among the longest-running TSBF projects.3.1. \"Research\" or \"action research\"?The development of social science at TSBF has been implicitly predicated on two very different models of how change is brought about in rural communities and what role outsiders and scientists can play in that process. The more conventional approach suggests that a \"good technology sells itself\" and that working with communities merely requires that the \"best bet options\" are made available to the \"categories of farmers\" who are likely to benefit from them. In this model, which is still widely held by many natural scientists including TSBF partners, a \"research\" organisation has too few resources and no comparative advantage in doing dissemination, and is better placed to research and evaluate the dissemination and technology promotion activities carried out by partners (local NGO's or national agricultural bodies). The alternate approach argues that understanding local processes of innovation, resource distribution, resource allocation decisions, and information transfer is essential to developing technologies relevant to their users' conditions. Integral to this second approach is the development of meaningful communication and learning across disciplinary boundaries -something that TSBF has attempted to do repeatedly, but which still remains problematic.As TSBF and its partners became more versed in participatory methods, tension has developed between these models. The desire for more \"development\" oriented activity has been highlighted in the redesigning of the \"Resource Integration\" theme of TSBF in 2000 into the new Focus 1, demonstratively titled \"Empowering Farmers\", into which all the other bio-physical Foci's arrows flow. It may also have been further accentuated by the recruitment in the late 1990s of TSBF field staff for Kenya with NGO backgrounds in action research. The argument has been that without actively engaging in dissemination and community organisation the phenomena of interest to research (knowledge flows, further innovation and adaptation, etc.) will be too scarce to be viable or observable. Indeed, these staff members have found it difficult to define or implement \"research\" as an independent activity, devoid of extension or development components.In reality, most partner organisations have lacked the resources (personnel, transport, and operating funds) to carry out such work, and indeed have often turned to TSBF for material or logistical support. The decision to devolve more of the research, experimentation, and dissemination activities to the host communities, therefore, is not so much ideologically driven as pragmatic. The increasing use of farmer-designed and farmer-run experiments, farmer-to-farmer training, and group-based activities has effectively begun to address the desire for more \"action\" oriented work while providing social processes worthy of investigation. What has emerged in the project areas of Western Kenya (where TSBF and local groups have had a reasonably long, 5-8 year history of contact) are prolonged, one-to-one relationships between scientists and farmers. Interactive, two-way learning, through community-based interactive sessions and farmer-based demonstrations, has been enhanced by researchers, and is widely conducted in local dialects. The ongoing challenge, however, has been finding optimal roles for researcher, extensionist, and farmer participation under these continuing conditions of resource constraint.Under the prevailing orthodoxy of participation, it is difficult to find projects that do not describe themselves as using and embracing \"participatory\" methods, to the extent that the term invites dismissal or covert cynicism (cf. Cooke and Kothari, 2001). These methods are usually assumed to apply only to relationships between researcher / extensionist and \"client\", where they are used to \"level\" the power relationships between actors. Yet in the TSBF context, where planning and implementation of activities is explicitly done in partnership with national research and extension institutions, participatory methods of collaboration have had to evolve. If cross-disciplinary learning has been difficult within TSBF, it has been even more so between TSBF and its partners, a fact which must be acknowledged before looking at the effectiveness of \"participation\" in the dealings of \"researchers\" with farmers.This point needs to be based on what might be called \"realistic expectations\" of change. True collaboration must recognise (however reluctantly) that working with the human resources that are on hand within networks means starting from the perceptions and skills of those partners and moving at the best pace possible. It would have been easy to \"cook\" fancy results about participation if the social scientists had simply gone it alone. Working in partnership through AFNET, however, has forced TSBF to confront the realities of public funded research in Africa, the conservatism and logistical difficulties of which demand considerable patience. It is relatively easy for partners to influence each other's rhetoric, harder to alter each other's conceptualisations of problems, and harder still to make lasting changes in the way each carries out research tasks. \"Participation\" is not an approach whose benefits are learned or appreciated quickly and the socialisation of knowledge backwards and forwards between scientists and farmers depends fundamentally on the generation of experience.The progress of AFNET towards \"internalising\" the rhetoric of farmer participatory research may seem glacially slow for being one of the more advanced scientific networks (cf. review of on-farm research in the EU-funded project, Carter et al., 1998). As mentioned above, the scarcity of AFNET members trained in participatory methods able to act as \"champions\", and the lack of continuity in many institutions facing financial crisis, hinder the development of a more interdisciplinary research culture.However, progress is being made in learning new attitudes and unlearning old ones. For example, the Zambian EU team decided to work on fundikila mound systems and to clear land on the research station to replicate the farmers' practices on-station, in full view of their peers. The Zimbabwean and Kenyan research teams have come to acknowledge the various micro-niches that farmers recognise and manage and have incorporated these into various research designs. Within the BMZ-funded project, increasingly sophisticated understanding of wealth and gender differences as they relate to soil fertility management have been incorporated into the project design. Finally, previously distinct elements of process and on-farm research have been combined in activities where complex soil-crop scenario modelling has been fed back into negotiation or decision support work conducted with farmers.It is, of course, never easy to surrender control of research agendas, even where the research is ostensibly for the benefit of the rural poor (i.e.: TSBF's Theme 1 is \"Empowerment of Farmers\" with new technologies). If TSBF has seemingly embraced what Ashby (1992) calls the \"devolution to farmers [or other stakeholders] the major responsibility for adaptive testing and sharing of accountability for quality control over research\", what have the political implications of this move been? Examples relating to defining innovation, the use of local youth as enumerators, and the micro-political dynamics of groups demonstrate.Farmer participatory research activities at TSBF began in community settings where portions of land were already being hired for research-designed activities, including both \"pure\" experimental treatments and \"demonstration\" plots to showcase the presence of nutrient deficiencies or the efficacy of various technologies. These activities tend to cloud the local understanding of what \"research\" actually is or can be, creating a sense that research generates information that \"important\" (the payments for land are known locally) but which comes in forms not readily accessible or understandable to \"ordinary people\". Even when experimentation has been ostensibly \"turned over\" to farmers, it is common to hear the new technologies being referred to as \"belonging to the researchers\".Beyond a basic unfamiliarity with the intentions and operationalisation of collaborative research with scientists, there is the problem that many of the \"experimentation\" activities undertaken do not provide ideal venues for farmers to innovate in ways familiar to them. TSBF has a goal of providing farmers with a \"basket of options\" for ISFM (Swift et al., 1994), including legume cover crops, improved fallows, biomass transfer (cut-and-carry) systems, improved compost manure, and various combinations of organic and inorganic fertilisers. In the EU and BMZ-funded projects, after initial PRA's in the communities, farmers were given the chance to select technologies from this \"basket\" to try on their own land. Selection of otherwise completed technologies, however, is not the same as participating in the technology design process.The \"over-designing\" of technologies before involving farmers in their development is a natural consequence of scientists failing to a) trust in the innovative capacity of farmers or b) know how to apply farmers' knowledge and innovation as contributions to \"formal\" scientific activity. It limits farmers' role to relatively passive activities, such as selecting niches or adapting application rates to local circumstances, which ultimately discourages any sense of ownership of the technology development process. However, to recognise certain behaviour as an \"innovation\" requires channels of communication and trust to exist between farmer and scientist, and a willingness to see all modifications of practice (including abandonment and complete reversals) as potentially useful.Observations of innovative farmer practice can feed into researchable topics, such as the use of Tithonia as a nutrient-rich mulch (now a staple \"technology\" promoted by TSBF and others in East and Southern Africa). When translating the Tithonia biomass transfer technology to other farms, a commonly heard comment is that the cut-and-carry system is \"labour intensive\". Harvesting biomass from hedgerows all at once before planting one's crops is indeed a large, and previously non-existent task, even if pruning hedgerows or applying plant material on cropland are familiar activities already in the household calendar. As a result, many farmers have begun harvesting their Tithonia sporadically (as part of normal hedge maintenance) and transferring it to their compost pile (another familiar task). Clearly the decision not to continue with the cut-and-carry operation and instead supplement the compost pile with Tithonia should be seen as an \"innovation\" or indeed as a logical supplementation of existing practices. However, while Tithonia had been identified as a \"best bet\" for direct application to fields since it decomposes so rapidly, it may not be the \"best\" option for materials to be added to compost piles that sit for a time before application. A natural entry point for truly interdisciplinary research is experimentation based on farmers' own practices (many report that Tithonia speeds the \"cooking\" of compost piles making it ready for use sooner) to validate the use of Tithonia or alternative materials as part of the composting process.Among the many tools and approaches used for conducting its social fieldwork in Western Kenya in the early 1990s, TSBF relied on young and literate people, recruited from the local communities as enumerators. They were typically given basic training that would allow them to support \"communitylevel\" research activities such as questionnaire administration and the setting up of trial plots. Over time, they began to take on more responsibilities, received further training, and by 1997 were facilitating farmer-led experimentation. However, personnel changes at TSBF, and attitudinal differences between TSBF on the one hand, and the local partner KARI (Kenya Agricultural Research Institute) on the other, had important implications for the role of these enumerators and the work they carried out.TSBF staff had viewed the capacity development of these youth as part of a community-based learning strategy to build rapport with other farmers. Indeed, the local communities were openly sympathetic to this approach, since it provided immediately tangible benefits to locals employed as enumerators, and also ensured that research was carried out by people who would be familiar to community-members and at home with the local cultural norms and vernacular. However, what was perhaps never explicit was how (if at all) the inclusion of these enumerators in project activities differed from the way that other local people were trained to work on experimental plots as paid labourers.During the interval between Carter's departure and Sikana's arrival, the enumerators carried on their work, with backstopping where possible from KARI. However, without strong advocacy for participatory methods rather than more top down approaches, KARI staff tended to view the enumerators who were already \"part of the community\" as a useful channel for \"passing useful scientific skills and knowledge into the local community\". At the same time, because of their training, regular association with TSBF staff, and the perceived status benefits accruing from their employment, many of the enumerators tended to count themselves more as part of TSBF than as part of the \"community\". In the end, this distancing between enumerator and community (enhanced by youth and the fact that many of the enumerators did not themselves farm) undermined their ability to link farmers and researchers effectively.Since then, an effort has been made to build individual capacity within KARI and the national extension service for participatory research. Currently, an agricultural extension agent who speaks the local dialects has been hired for facilitating community-based input. This expert works with community groups, farmer field schools (FFS), individual farmers and other local stakeholders. However, as with the enumerators, these activities have demanded considerable backstopping by the Social Science Officer and other disciplines within TSBF.As TSBF placed more attention on building capacity in its partners for farmer participatory research, it also shifted to working with local farmers as groups and individuals. In the earlier 1990s, onfarm trials were based on individual's farms. In such arrangements, host farmers were expected to define and explain experiments to other local and visiting farmers. While we do not know the exact accomplishment through this arrangement, there are indications in Kabras and Vihiga that selecting \"model\" farmers to work with disaffects them from many other farmers. Down the road, focus shifted to the group approach. Initially, it seemed obvious that involving many farmers would have a multiplier effect. However, it soon became apparent that the manner in which TSBF talks to whom is more important than mere numbers. Groups are on frequently unstable and many are not especially open to new membership. When researchers request farmers to work with them collectively, \"new\" groups emerge. But these \"new\" groups usually comprise members of a previous, defunct group. This means that one has to deliberately seek the inclusion of all types of farmers (within and outside groups) in research and dissemination. This role of a local unifier is tricky and can even appear comical before local farmers.Intervening research on the nature of social capital and the role of local groups and networks in passing agricultural information (Misiko, 2001) has shown that there is still a tendency for some groups or individuals to view their participation in TSBF as \"secret knowledge\" that is not to be shared with others. Likewise, non-participants are often wary of inquiring about project activities, assuming that they are not welcome or need to be invited by some patron. This attitude has persisted for multiple reasons, and in spite of the considerable efforts of TSBF and other research bodies to present their work as \"open to all\" by actively seeking to include marginalized groups. Because local politics takes precedence even over the \"good intentions\" of outsiders, the vast exposure that many farmers have had to project work in Western Kenya does not, therefore, translate into widespread use or understanding of ISFM.The initial willingness of TSBF to accept \"groups\" as representatives of community interests has led to numerous problems. After all, groups exist and persist when they have strong roles and identities, histories of their own which often only become known with time. For example, the most vocal members of groups have frequently been people who are either not well respected by others locally, or possessed of agendas that run far beyond ISFM. This later group tends to see the research project as a vehicle for access to new resources and political leverage than as an opportunity for new learning (Sikana, 1995), although it may take project staff a long time to appreciate this reality. Since much of TSBF's on-farm work has been initiated in the context of structural adjustment programmes and the cessation of donor funding for major local development projects, it is natural that farmer concerns about water, health, poor infrastructure, or education would be mapped onto the \"research\" activities if TSBF was the only \"development\" agency working in their area. Beyond such explicit \"hijacking\" of groups, there are frequently tensions between participants over the definitions of goals, membership, and indeed the \"success\" of the group's activities.Nevertheless, working through groups provides an opportunity to diffuse risk and broaden responsibility and ownership of activities. Groups should be seen neither as a panacea for communitybased management's difficulties, nor as a replacement for effective dissemination strategies. When setting up experiments or demonstrations at the local level, having wider input about where in the landscape, whose land, or which soils are suited to which types of research activity has proven invaluable. With our broadened knowledge of the diversity of local soil types, requests by farmers to have activities replicated on different soils become logical and understandable, when previously they might have been dismissed as unjustified demands for a share of a perceived research \"pie\". In the end, such replication turns out to be both good science and good politics.The challenges that TSBF has tried to address are highly complex in both biophysical and social terms. As such, interdisciplinary collaboration depends on developing a better understanding of what changes are taking place, and of developing a modus operandi that can generate useful knowledge as part of an on-going dialogue between scientists and farmers.The parallel dialogue that must take place, between social and natural scientists, has been easiest around themes that integrate themselves readily into natural science work, including spatial variability, wealth ranking and ISFM practice, and the importance of understanding the strengths and weaknesses of existing local knowledge. It has been considerably harder to incorporate elements that relate to the political nature of \"research\", such as using livelihoods analysis or feminist political ecology to find the place of ISFM and research interventions within local practice.If AFNET collaborators have been slow to adopt interdisciplinary and participatory approaches, it is due in part to the relative lack of successful, convincing models of how such approaches pay short or long-term benefits to NRM research. Further constraints have been staff turnover (which leads to fragmented agendas and loss of institutional memory), scarcity of time and resources, and a shortage of generalists or social scientists within partner organisations. The rhetoric of interdisciplinarity and participation have rapidly infiltrated research bodies because they are relatively cost free and often there is the perception that donor funding is linked to such language. Simplified versions of interdisciplinary activities, linking ISFM with participatory wealth ranking, or moving from local soil taxonomies to broader understanding of how soil fertility is managed locally, have also begun to take hold within local practice. While some natural scientists are \"afraid of having to become social scientists\", there is a slowly growing constituency within AFNET that sees advantages for interdisciplinary collaboration. Nevertheless, without relatively senior \"champions\" for interdisciplinary or socially oriented approaches within TSBF, new methods and approaches are at a disadvantage compared with the more familiar status quo.Given the variables of donor climate, institutional and personnel changes, and socio-political change on the ground, truly interdisciplinary INRM research will need to develop a common language and common priorities that can form a core identity in dealing with outside forces. This requires an iterative process of negotiating the role of \"research\" in the development of local communities. If donors, researchers, and extensionists feel the need to \"scale up\" local successes and achievements to broader communities, it must be reconciled with the desires of the initial community members for taking research accomplishments to greater depth. If moving towards group-based research methods means shifting the burden of implementation to national partners, a common path for \"participation\" will need to be negotiated. In particular, the skills and attitudes necessary to support more decentralised forms of research need to be cultivated by the scientists, agents, and farmers involved.Despite the rhetoric of interdisciplinary collaboration, cross-disciplinary learning and communication remain complicated by the divergent ideas of what role \"research\" can and should play in bringing about change in rural communities. Resolving these divergences often falls to social scientists, since their disciplinary orientation predisposes them to thinking about such issues and their colleagues are more likely to see these issues as somehow separate from their daily activities of research. However, building common bodies of knowledge and practice can only happen with the full participation of all disciplines involved in INRM. If we look at how far an organisation like TSBF has come in ten years, from research foci that concentrated on the integration of biological processes to ones which now embrace the livelihoods and knowledge of the farmers who practice integrated soil fertility management, there is room for hope.The cycling of nutrients through the decomposition of plant residues is important in all ecosystems. However in the soil fertility management of many tropical farming systems, organic sources play a dominant role because of their short-term effects on nutrient supply to crops (Palm et al., 2001). There is now a considerable literature reporting decomposition and nutrient release patterns for a variety of organic materials from tropical agro-ecosystems. This information has been drawn together so that it can be used for improvement of soil fertility through better management of organic inputs (e.g. Giller and Cadisch, 1997;Palm et al., 2001), and understanding has emerged of how resource quality factors influence the release patterns.In nutrient and capital poor tropical farming systems, effective use of whatever nutrient sources are available will be required to raise and maintain productivity (Giller et al., 1997). If models are to be useful in helping to design farming systems that use various nutrient sources more effectively, it is a first requirement that the models must be able to reliably describe the release of nutrients from the different organic sources. Palm et al. (1997) pointed out that there is little predictive ability for making recommendations on combined use of organic and inorganic nutrient sources. One reason for this is the inability of models to adequately capture the short-term dynamics of the release of nutrients from organic materials.In this paper we report on how one particular model, APSIM (Agricultural Production Systems Simulation Model, McCown et al., 1996;Keating et al., 2002), represents the decomposition of organic inputs, and how the quality of the inputs influences nitrogen release. The manner in which the dynamics of soil carbon and nitrogen are modelled in APSIM's SoilN module (Probert et al., 1998) is similar to what is found in many other models -see reviews by Ma and Shaffer (2001) and McGechen and Wu (2001). Models do differ in the pool structure used to describe the decomposition of organic inputs, with the pools differing in their rates of decomposition. However, we are unaware of any model where the pools differ in chemical composition, with the effect that inputs decompose with non-varying composition. We show that the assumption that all pools have the same C:N ratio fails to adequately represent the observed behaviour for release of N from some organic inputs. We present a modification of APSIM SoilN which allows for different C:N ratios in each pool. The modified model was able to better match the mineralization/immobilization of N observed in laboratory incubation studies.The development of the APSIM SoilN module (Probert et al., 1998) can be traced back via CERES models (e.g. Jones and Kiniry, 1986;Godwin and Jones, 1991) to PAPRAN (Seligman and van Keulen, 1981). Briefly, crop residues and roots added to the soil, are designated fresh organic matter (FOM) and are considered to comprise three pools (FPOOLs), sometimes referred to as the carbohydratelike, cellulose-like and lignin-like fractions of the residue. Each FPOOL has its own rate of decomposition, which is modified by factors to allow for effects of soil temperature and soil moisture. For inputs of crop residues/roots it has usually been assumed that the added C in the three FPOOLs is always in the proportions 0.2:0.7:0.1. In this manner the decomposition of added residues ceases to be a simple exponential decay process as would arise if all residues were considered to comprise a single pool.Although the three fractions have different rates of decomposition, they do not have different compositions in terms of C and N content. Thus whilst an input might be specified in terms of the proportion in each of the FPOOLs, thereby affecting its rate of decomposition, the whole of the input will decompose without change to its C:N ratio. If the analogy can be made with the dissolution of a substance, we might say that the whole of the residues decompose congruently. Alternatively the system can be described as having three soil organic C pools but only one soil organic N pool (Gijsman et al., 2002).The release of N from the decomposing residue is determined by the mineralization and immobilization processes that are occurring. The C that is decomposed from the residue is either evolved as CO 2 or is synthesized into soil organic matter. APSIM SoilN assumes that the pathway for synthesis of stable soil organic matter is predominantly through initial formation of soil microbial biomass (BIOM), though some C is transferred directly to the more stable pool (HUM). The model further assumes that the soil organic matter pools (BIOM and HUM) have C:N ratios that are unchanging through time. The formation of BIOM and HUM thus creates an immobilization demand that has to be met from the N released from the decomposition of the residue and/or by drawing on the mineral N (ammonium-and nitrate-N) in the system. Any release of N during the decomposition process in excess of the immobilization demand results in an increase in the ammonium-N. The model operates on a daily time step, so that decomposition of the residue fractions is happening simultaneously with decomposition of the soil organic matter pools.If we ignore the dynamic nature of the system, the N mineralization from a substrate can be expressed succinctly as (Whitmore and Handayanto, 1997):where Z is the C:N ratio of the decomposing substrate, E is a microbiological efficiency factor which can be taken to be 0.4 (the value in APSIM SoilN for the fraction of the decomposing carbon that is transformed into soil organic matter), and Y is the C:N ratio of the soil organic matter being formed. Equation (1) implies that there is a C:N ratio of substrate that determines whether decomposition results in net N mineralization or immobilization. Assuming the initial product of decomposition is soil microbial biomass with Y = 8 (the value used in APSIM SoilN), the critical value can be calculated as 20. As shown by Whitmore and Handayanto (1997), this expression accounts for much of the variation found in the data that have examined N mineralized (or immobilized) in relation to the C:N ratio of the added organic matter.The rate of net N mineralization is dependent on the rate of decomposition. Thus allowing the pool sizes of the three FPOOLs to be an input that characterizes the type of organic input will alter the rate of net N mineralization (as shown by Quemada and Cabrera, 1995;Quemada et al., 1997). However changing the pool sizes alone cannot alter whether a source exhibits initial net N mineralization or immobilization (since this is determined by the C:N ratio of the source).In studies of the mineralization of N from various manures, Kimani et al. (2001) and Delve et al. (2001) encountered situations where there was an initial immobilization of N, despite the fact that the overall C:N ratio of the material was such that it would be expected to result in net mineralization. This behaviour can not be modelled without assuming that the three FPOOLs also differ in their C:N ratios.Modifications were made to the APSIM SoilN module so that any input of organic material could be specified in terms of both its fractionation into the three FPOOLs, and the C:N ratios of each FPOOL. In the modified model, each FPOOL is assumed to decompose congruently. The rates of decomposition of the three FPOOLs were not changed from the released version of APSIM (0.2, 0.05 and 0.0095 day -1 respectively under non-limiting temperature and moisture conditions).Using this enhanced version of the model, we have explored the effects on simulated N mineralization from hypothetical sources that differ in respect of firstly, their fractional composition (the proportion of C in the 3 FPOOLs), and secondly, the C:N ratios of the FPOOLs.The effects are illustrated by contrasting four assumptions as to how an organic input decomposes:1. using the released version of ASPIM SoilN (v 2.0) 2. changing the fractional composition of the FPOOLs but with the C:N ratio being the same in all pools 3. changing the FPOOLs to have different fractional compositions and different C:N ratios, in the first instance with FPOOL1 differing from a common value for FPOOLs 2 and 3 4. with the fractional composition and C:N ratios differing between all 3 FPOOLs.The enhancements made to the model result in extra information being needed to specify the inputs. Ideally it should be possible to derive the necessary information from known (measured) properties of the organic sources.The experimental data reported by Delve et al. (2001) have been used to investigate whether the analytical data for a range of feeds and faecal samples can be used to specify the model to simulate the N mineralization measured in a laboratory incubation experiment.The model was configured to simulate a simple incubation study, involving a single layer of soil under conditions of constant temperature (25 o C) and at a soil water content that ensured there was no moisture restriction on decomposition. Initial nitrate-N concentration in the soil was 20 mg N kg -1 . The effect of different organic inputs was investigated by incorporating materials that contained a constant amount of N (100 mg N kg -1 soil) but with varying C:N ratio. A control system was also simulated without any added organic input.The output from the simulations are presented as net mineralization/immobilization expressed as a percentage of the N added: N mineralization (%) = 100 x (Mineral-N input -Mineral-N control )/N added where Mineral-N input is the simulated ammonium-+ nitrate-N in systems with the added source, and Mineral-N control in the absence of any input.The model was specified to simulate the incubation study of Delve et al. (2001). Using a leaching tube incubation procedure (Stanford and Smith, 1972), they measured net N mineralization for feeds and faecal samples resulting from cattle fed a basal diet of barley straw alone, or supplemented with 15 or 30% of the dry matter as Calliandra calothyrsus, Macrotyloma axillare or poultry manure. The soil used was a humic nitisol with organic C content of 31 g kg -1 , C:N ratio of 10 and pH (in water) of 5.9. The incubations were conducted at 27 o C.Data were reported on the chemical composition of the feeds and faecal samples including: total C and N; water soluble C and N; acid detergent fibre (ADF), neutral detergent fibre (NDF) and acid detergent lignin (ADL) (van Soest et al., 1987).Experimental data (Kimani et al., 2001), that indicated the need to reconsider how N mineralization from organic inputs is modelled, are illustrated in Figure 1. For a wide range of manures, their results consistently show an initial immobilization or delay in mineralization lasting several weeks, even for materials that have overall C:N ratios of less than 20. This pattern of response is noticeably different to studies of N mineralization from plant materials (e.g. Constantinides and Fownes, 1994); plant materials with low C:N typically exhibit positive net mineralization from the commencement of the incubation period.Other authors also report initial N immobilization followed by net mineralization in experiments with animal manures having low C:N ratios (Trehan and Wild, 1993;Olesen et al., 1997). The faecal samples studied by Delve et al. (2001), with C:N ratios in the range 20-27, had even more complex patterns of mineralization; some materials showed initial net mineralization before an extended period of immobilization lasting for at least 16 weeks of incubation (see below). Simulation of mineralization for sources with different C:N ratios using the released version of APSIM SoilN is shown in Figure 2. The results are in general agreement with experimental studies for plant materials where net N mineralization is closely related to the N content and hence C:N ratio (e.g. Constantinides and Fownes, 1994;Tian et al., 1992). For sources with C:N < 20, net mineralization occurs from the outset (as predicted by equation 1). However with C:N > 20, there is initially immobilization of mineral-N and it is only as newly formed soil organic matter is re-mineralized that mineral-N in the system begins to increase.The lower pane of Figure 2 shows the same data plotted against the C:N ratio for different periods of incubation. Again the pattern of response is familiar from experimental data that have been used to infer the C:N ratio of a substrate, around 20-25, that determines whether net mineralization or immobilization occurs. The simulation results show that the C:N ratio of the substrate that results in zero net mineralization changes with the period of incubation, increasing from approximately 21 at day 10 to 26 at day 100. Such an effect has not generally been recognized when discussing critical C:N ratios with respect to mineralization/immobilization, though its importance was recognized by De Neve and Hofman (1996). Thus incubation period is a factor that will complicate efforts to compare results from different incubation studies. Furthermore other aspects of the incubation conditions can also be expected to have similar effects as the incubation period; in particular higher incubation temperature is likely to have much the same effect as increasing the incubation period.The effect of changing the pool structure of the input by modifying the fractions in each of the FPOOLs is illustrated in Figure 3. For inputs with low C:N (<20), a greater proportion of material in the FPOOLs with lower rates of decomposition simply slows the release of mineral-N. Where C:N is >20 so that net immobilization occurs, inputs with a greater proportion of material with lower rates of decomposition result in less immobilization during the early stages of decomposition, but it also takes longer before the system exhibits positive net mineralization. It is to be noted that simply changing the proportions of the input between the three pools with unaltered C:N ratio can not cause a switch from causing net mineralization to immobilization, or vice versa. Effects of changing the composition of the input by modifying the C:N ratios of the different FPOOLs are shown in Figures 4 and 5. In Figure 4, all materials have the same overall C:N ratio, but the C:N ratio of FPOOL1 is now greater than for the material in pools 2 and 3. The result is that the material in FPOOL1 which decomposes most rapidly creates an immobilization demand, and the higher the C:N ratio the greater the initial immobilization. However if C:N of FPOOL1 is higher, there must be compensating decreases in the C:N ratios of the other pools. As incubation time increases, the differences between different materials decrease so that there is little longer-term effect of the C:N ratios of the FPOOLs on net mineralization which is determined largely by the overall C:N ratio. Figure 5 illustrates variation in the C:N ratio between FPOOLs 2 and 3. Again all materials have the same overall C:N ratio and here the C:N of FPOOL1 is also fixed at 10. With the low C:N in the rapidly decomposing pool, there can be an initial net mineralization, especially when the C:N of FPOOL2 is also relatively low. However, as FPOOL1 is depleted, there can be a switch from net mineralization to net immobilization. Increasing the C:N of FPOOL2 results in increasing immobilization and the immobilization persists to longer times. The modelled net mineralization from hypothetical sources display patterns of N release that are similar to published experimental data. Notably the several weeks delay before mineralization became positive, as exhibited by several of the manures studied by Kimani et al. (2001), is consistent with variation in the C:N ratio of FPOOL1 (Figure 4). On the other hand, the longer delay reported by Delve et al. (2001) is more like the pattern shown in Figure 5 associated with variation in FPOOL2 and 3.We have attempted to use the analytical data reported by Delve et al. (2001) to specify the \"quality\" aspects of organic inputs represented in the model. We assume the soluble components of C and N equate to FPOOL1; thus the analytical results are sufficient information to determine the proportion of total C in this pool and its C:N ratio. Also we assume that ADL, which measures lignin, equates to FPOOL3 permitting the fraction of C in this pool to be estimated; the fraction of C in FPOOL2 is found by difference. Since the overall C:N ratio (on a total dry matter basis) is also known, the only missing information is the distribution of non-water soluble N between pools 2 and 3. A series of simulations were carried out for each source with the different combinations on C:N in the two pools (constrained by the C:N of the total DM).Figure 6 shows the simulation of N mineralization for the control treatment. Although there is a slight under-prediction, the general pattern agrees well with the measured data. It is to be noted that in the model the net N mineralization from an organic source is only influenced by the control treatment when there is inadequate mineral N in the system to meet an immobilization demand.The net N mineralization for the feeds and a selection of the faecal samples studied by Delve et al. (2001) is shown in Figure 7. The outputs from two simulations are compared, these being the outputs from the modified and unmodified versions of the model. The input data used for the modified model are set out in Table 1. Delve et al., 2001). Experimental data shown as symbols with bars representing standard errors. The heavy broken line is for the model where all organic material is assumed to decompose with the same C:N ratio; the continuous line is for the model with different C:N ratio in each FPOOL. Parameters used to specify the different sources (proportion of C and C:N in the three FPOOLs) are set out in Table 1. Net mineralization (%)Table 1. Composition of organic materials (feeds and faecal samples) used for simulating the mineralization study of Delve et al. (2001). 1 simulated N mineralization was not sensitive to partitioning of N between pools 2 and 3 2 value in parentheses denotes proportion of supplement in dietFor most of the materials the goodness of fit is substantially better for the modified than for the unmodified model. Using the analytical data to specify the fraction of C in each of the FPOOLs and the C:N ratio of FPOOL1, it was possible to choose values for the C:N ratios of FPOOL2 and FPOOL3 to obtain satisfactory fits with the measured data.In general the fit is better for the faecal samples than for the feeds, with the poorest fit for the poultry waste. The pattern of net mineralization measured for the poultry waste, which had an overall C:N ratio of 17, is different from the other materials in that the change from immobilization to mineralization that occurred after 50 days was not maintained, and further net immobilization occurred later in the incubation. The simulation for the barley straw (C:N 86) predicts that immobilization continues for at least 200 days. Because all mineral N is immobilized in this treatment, the simulated immobilization is determined by the rate of mineralization of the control treatment and is not sensitive to how N is partitioned between FPOOLs 2 and 3. The under-prediction of N mineralization in the control treatment (Fig. 6) is the cause of the under-prediction of net immobilization by the barley straw.The essence of equation ( 1) is built into many dynamic simulation models that describe the decomposition of organic residues and the associated mineralization of N. Such models are capable of capturing the gross effect of C:N ratio (as illustrated in Fig. 2) on mineralization/ immobilization from plant residues.However they are not able to represent the more complex pattern of mineralization/immobilization that has been reported from laboratory incubation studies of N release from manures with low C:N (e.g. Fig. 1). To capture this pattern of N release it is necessary to conceptualize the organic input as comprising discrete fractions that differ not only in their rates of decomposition but also in their chemical (i.e. C and N) composition.The observed behaviour suggests that the fraction of the substrate that decomposes fastest has a higher C:N ratio than the bulk of the material. If the portion that decomposes fastest can be equated to the water-soluble fraction, this is consistent with the analytical data of Kimani et al. (2001) (Table 2). Their data show that the soluble component, which amounted to some 12% of the total carbon, had a much higher C:N ratio than the materials as a whole. 3.8 (1.9) 68 ( 60)1 soluble C expressed on total DM basis (i.e. average of 12% of total C was measured in the soluble fraction)In contrast, the mineralization data of Delve et al. (2001) (Fig. 7) and chemical composition of their materials (Table 1) indicate that the measured water-soluble component had a smaller C:N than the bulk materials. To simulate the observed mineralization data it was necessary to assume that the materials had higher C:N in FPOOL2 than in FPOOL3.To some extent, this difference between in the C:N of the water-soluble components in the two studies can be explained by the nature of the manures. Those in the study of Delve et al. (2001) were fresh faecal material, whereas the manures studied by Kimani et al. (2001) had been collected from farm situations where they would have been exposed to varying degrees of weathering what would have been expected to remove some water-soluble components. However if this is the explanation, we are unable to satisfactorily account for why the analytical data of Kimani et al. (2001) should still indicate considerable amounts of water-soluble C, nor why there should have been preferential loss of N relative to C resulting in increased C:N for the water-soluble components.By simulation of hypothetical materials, we have shown that the model can be parameterised to simulate the general pattern of N mineralization that is observed for various organic sources. Nonetheless, it remains a challenge to know how appropriate parameters should be selected for a given source and/or how to derive the parameter values from other information that may be available as analytical data for supposed \"quality factors\". Here we have used data for C and N in the water-soluble components to specify FPOOL1, and the measured ADL to specify the C in FPOOL3. To obtain the goodness of fit shown in Fig. 7 for the manures required C:N in FPOOL2 in the range 36-66, with corresponding C:N in FPOOL3 of 9-10 (Table 1). Attempts to estimate the C:N of FPOOL2 from measured data for N associated with ADF and NDF (Delve et al., 2001) produced values that were considerably higher (range 63-174 for the manure samples) with the corresponding values for pool 3 becoming very narrow (<0.8); the goodness of fit for simulations of N mineralization using these values were substantially worse than those shown in Fig. 7.For the feed materials (Calliandra, Macrotyloma, poultry waste), the predictions were less good than for the faecal samples. To obtain a reasonable fit in the early stages of the mineralization a high C:N in FPOOL2 is required, but this results in very low values for FPOOL3 and over-prediction as the incubation period progresses beyond 100 days.The resource quality factors that have been shown to influence N release from organic sources are the C:N ratio (or N concentration in plant materials for which C concentration varies little), lignin and polyphenol concentrations (Palm et al., 2001). These studies suggest that the effect of lignin is consistent with the concepts in the model in as much as higher lignin content can be represented by a greater proportion of the C in the slow decomposing pool. But it is also necessary to hypothesize that the FPOOLs differ in their C:N ratio. The polyphenol concentration in the materials studied by Delve et al. (2001) were low (<1.6%) except for the Calliandra feed. It remains uncertain how the effects of polyphenols on decomposition and N mineralization can be represented by the model. The beneficial effects of soil organic matter management in the tropics have been amply documented as far as they relate to soil functions such as nutrient release (nitrogen and phosphorus in particular) and soil architecture (Vanlauwe et al., 1998;Nziguheba et al., 2000;Feller and Beare, 1997). Much less information is available on the relations between soil organic matter changes and the associated changes in nutrient retention capacity for neither cations nor anions. Nevertheless, soil organic matter is known to contribute to the total charge of a soil, a charge that is mostly pH-dependent. As a consequence, empirical relations do exist that predict soil cation exchange capacities based on soil organic carbon concentrations (Manrique et al., 1991;Asadu et al., 1997;Krogh et al., 2000). In highly weathered soils, the creation of extra charge, on top of the one derived from soil mineral components, can be an important management goal as CEC values can be increased by factors from values as low as 1 cmol c kg -1 soil to 4 -6 cmol c kg -1 soil (Gallez et al., 1976;Oades et al., 1989). Apart from a general idea on soil organic matter dynamics, we do not have a precise idea on how fast we can positively affect charge characteristics and in which size fractions the impact is most strongly seen. In the present paper we present a dual approach. First, we will investigate the changes in charge brought about by a 20-year continuous input of tree litter of known quality in an attempt to relate litter quality with the ensuing changes in soil charge characteristics. Secondly, we will address an important and ensuing management issue in this that it is crucial to determine how fast the changes in charge come about by an input of litter of a given quality and also, how long lived these changes are.For the first part of the experiment, we relied on a 20-year old arboretum established in 1979 on a ferric Lixisol (WRB, 1998) at the International Institute of Tropical Agriculture (IITA) in Ibadan, South Western Nigeria (3°54'E and 7°30'N) where we collected soil samples under seven multi-purpose trees: Afzelia africana, Dactyladenia barteri, Gliricidia sepium, Gmelina arborea, Leucaena leucocephala, Pterocarpus santalinoides and Treculia africana. For more information on this site we refer to Oorts et al., (2000) and Kang and Akinnifesi (1994). We took soil samples in March 1999 from the surface horizons (0-10 cm) of the corresponding plots by taking 4 cores (10 cm depth, 10 cm dia) from each alley at random throughout the alley. Samples from different alleys were considered as field replicates. Litter was collected from the soil surface before soil sampling and leaves from the respective trees collected. Both litter and leaves were air dried before analysis. For the field incubation experiment, decomposition tubes (10 cm depth, 10.5 cm dia) were filled with topsoil (0-10 cm) of a ferric Lixisol (WRB, 1998) sampled at the I.I.T.A. campus and were installed in an adjacent plot. They were either kept bare or were amended with litter derived from Afzelia, Dactyladenia, Gmelina, Leucaena and Treculia. In the amended treatments soil was mixed with litter at an addition rate of 15 tons dry matter/ha in the top 10 cm of each core. Destructive sampling took place at 3, 6, 12 and 23 months after application. In both experiments, all soil samples were air-dried, and passed through a 4 mm sieve to remove roots and large stones before fractionation and analyses.The soil organic matter fractions from the arboretum samples and decomposition tubes sampled at 6 and 23 months after application were obtained by size separation after ultrasound dispersion. To this end, soil suspensions (25g soil and 125 ml distilled water) were subject to a 10 minutes sonication treatment at 62.5 W (=1500 J g -1 soil) with Misonix Sonicator XL2020. The soil suspension so obtained was then separated into the following size classes: > 2 mm, 0.25-2 mm and 0.053 -0.25 mm using wet sieving (Fritsch analysette 3, 50 Hz, 1.5 mm amplitude). The fractions on the sieve were collected and further split into mineral and organic components through flotation-decantation on water. Material smaller than 0.053 mm was collected and manually sieved through a 0.020 mm screen. The fine silt fraction (0.002 -0.20 mm) was separated from a subsample by sedimentation (four cycles) and the clay fraction (< 0.002 mm) collected from the respective supernatants by flocculation with CaCl 2 (± 0.02 M). The clay fraction was next washed salt-free by dialysis (Spectra/Por 4, MWCO 12-14.000). All fractions were dried overnight at 60°C and weighed. The above separation scheme resulted in 9 fractions: 2-4 mm mineral (M2000), 2-4 mm organic (O2000), 0.250-2 mineral (M250), 0.250-2 organic (O250), 0.053-0.250 mineral (M53), 0.053-0.250 organic (O53), 0.020-0.053 (coarse silt), 0.002-0.020 (fine silt) and < 0.002 (clay). Dry weight recoveries over the different samples ranged between 98.1 and 99.6 %.Soil pH was always measured in a 0.01 M CaCl 2 solution at a 1:5 soil:solution ratio after 1 h shaking. Organic carbon and nitrogen contents of soil and plant samples were determined using a CN analyser-mass spectrometer (ANCA-GSL preparation module and 20-20 Stable Isotope Analyser, Europa Scientific) after ball-milling. Plant material was analysed for lignin and (hemi)-cellulose content by the acid detergent method (Van Soest, 1963;Van Soest and Wine, 1967). Polyphenolics were determined by a revised Folin-Denis method (King and Heath, 1967). To account for the specifics of highly weathered soils, we used a CEC method designed to operate at in situ soil pH and at low ionic strengths. An unbuffered AgTU (silver-thiourea complex) solution (0.01 M Ag + , 0.1 M TU) was used to measure CEC and base saturation at prevailing pH of the whole soil samples from the arboretum (Pleysier and Juo, 1980). Variation of CEC with pH was determined on whole soil samples and the three smallest size separates. In short, (a full description of the method is found in Oorts et al., 2000) subsamples were weighed in centrifuge tubes and pH was increased by shaking for 2 h with 15 ml 10 -3 M NaOH. Next, 15 ml unbuffered AgTU (final concentration: 0.01 M Ag + , 0.1 M TU) was added and after shaking overnight, pH was recorded, samples were centrifuged and a first 1 ml subsample was taken from the clear supernatant for Ag analysis by AAS. Subsequently, the soil was gradually acidified by adding small amounts of 1 M HNO 3 and after each equilibration, pH was measured and a subsample taken from the supernatant for Ag analysis. The whole procedure resulted for each sample in 6 CEC measurements between pH 2.5 and 7.5.Soils in the arboretum were predominantly sandy, with an approximate composition of 79% sand, 13% silt and 8% clay. The largest soil organic carbon concentrations were observed in the Dactyladenia, Leucaena and Treculia stands ranging between 10.79 and 13.62 g C kg -1 soil (Table 1). The four other soils had comparable and considerably lower carbon concentrations in a range of 7.16 to 7.97 g kg -1 . CEC values at prevailing soil pH ranged correspondingly between 4.51 and 6.47 cmol c kg -1 for the three top stands and between 2.80 and 3.90 cmol c kg -1 for the four others that were lower in carbon. Most of the variation in CEC could be explained by the differences in carbon content, while an additional part of the CEC variation was explained by the pH. CEC = 0.15 + 0.43* C (g kg -1 ) n = 28, R 2 = 0.767, P<0.001 (1) CEC = -6.97 + 1.25 pH + 0.41*C (g kg -1 ) n = 28, R 2 = 0.870, P<0.001 (2)Also when CEC values were obtained at different pH values, most of the variation could be explained by differences in organic carbon concentration and pH. These two together explained 85% of the variation. CEC = -1.79 + 0.50*pH + 0.36*C (g kg -1 ) n = 168, R² = 0.849, P<0.001 (3)This allows concluding that in these soils the concentration of organic carbon is the main source of variation between the different treatments. The regressions between soil carbon content and CEC allowed calculating values for the CEC of the soil organic matter. From equation (1) it can be seen that values are obtained in the order of 430 ± 50 cmol c kg -1 C, at a pH of 5.8 which is the average in situ pH.The CEC of the coarse silt, fine silt and clay fractions increased with decreasing particle size (clay > fine silt > coarse silt), except for Treculia, Dactyladenia and Leucaena, where the fine silt had comparable or higher CEC values than the clay fractions. Clay and fine silt fraction had the highest contribution to the CEC of the whole soil, together they were responsible for 76 to 90% of the CEC of the soil at pH 5.8 (Table 2). The contribution of the fine silt fraction to the CEC at pH 5.8 ranged from 35% to 50%. For the soils under Treculia and Dactyladenia, this fine silt fraction had the highest contribution. The coarse silt fraction contributed 9 to 15% of the CEC. The recovery of the CEC in the fractions ranged from 95 to 104%. In Table 2, also the changes in organic carbon for the three main size separates are given. In general, the carbon concentrations of both silt fractions followed the same trends as the whole soil samples, with largest values for Dactyladenia, Treculia and Leucaena.As for carbon, the treatment effects on CEC were clearly present in the silt fractions, while the clay fractions were rather similar (Figure 1). The CEC values for the clay fractions varied between 15 and 20 cmol c kg -1 at pH 3 and between 24 and 32 cmol c kg -1 at pH 7. The variation in CEC values for the clay fraction could be explained for 83% by pH only, confirming the absence of a treatment effect through the residue application on CEC values of the clay fraction. Contrary to this, the CEC values of the fine silt fraction were highly dependent on the treatment and pH could only explain 24% of the variation. Carbon concentration and pH together explained 95% of the variation in CEC of the fine silt fraction. The same was true for the coarse silt fraction: pH alone explained 18% of the variation and pH together with carbon concentration explained 90%. It confirms that changes in CEC due to residue management are seen in silt fractions rather than in clay fractions, at least at this time scale.Soil in the decomposition tubes had a similar sandy texture as the arboretum soils: 76% sand, 16% silt and 8% clay. The results in Table 3 show that due to the single application of residues, important differences in soil carbon content and CEC were obtained and still obvious after up to 23 months after application. After 6 months, the residue application resulted in an increase in total soil organic carbon content from 4.22 g C kg -1 soil in the control to concentrations up to 6.07 g C kg -1 soil in the soil amended with Treculia, six months after application. The change in organic carbon content was the largest for this species and decreased from Treculia>Dactyladenia>Gmelina>Leucaena>Afzelia. Correspondingly CEC values decreased from 2.75 cmol c kg -1 soil for the Treculia amendment to 2.07 cmol c kg -1 soil for Afzelia, only slightly larger than the 1.92 cmol c kg -1 soil for the control. The carbon concentrations in the fine silt fraction, six months after amendment, followed the same trend, while the largest values were obtained for Dactyladenia and Treculia, and the smallest for Afzelia. CEC values of the fine silt fraction could be separated into two groups: on the one hand Treculia, Gmelina and Dactyladenia with values between 10.49 and 10.66 cmol c kg -1 fine silt and on the other hand Leucaena and Afzelia with values of 8.76 and 8.41 cmol c kg -1 fine silt respectively. Still, all residue treated soils had larger CEC values in the fine silt fraction than the unamended soil with 7.89 cmol c kg -1 fine silt. Also for the clay fraction after six months, the organic matter concentrations seemed larger in the amended soils than in the control (values between 28.48 and 30.67 g C kg -1 clay for the amended soils and 25.48 g C kg -1 for the control). However, the CEC values were similar for treated and untreated soils in a range of 19.35 to 21.25 cmol c kg -1 clay. After 23 months, the effects were still present in some treatments, while a decrease in carbon concentrations was obvious, both in control and amended soils. For the whole soil, the same order as at 6 months was still visible with Treculia still displaying the largest carbon content (5.25 g C kg -1 soil) and Afzelia and Leucaena becoming similar as the control soil with carbon concentrations between 3.76 and 3.85 g C kg -1 soil. CEC values for the whole soil at 23 months were also still larger than the control for Treculia and Gmelina, while they were similar as for the control for Leucaena, Afzelia and Dactyladenia. Treatment effects could much more clearly be seen in the fine silt fraction. For Treculia, Dactyladenia, Gmelina and Leucaena values were observed between 20.60 and 26.49 g C kg -1 fine silt in contrast to the values of 18.57 and 18.26 g C kg -1 fine silt for the Afzelia and control soils respectively. Trends in CEC values of the fine silt fraction followed the lines set by the organic matter concentrations: larger values for the silt fractions derived from Treculia and Dactyladenia than for those obtained from Gmelina and Leucaena, in turn larger than for Afzelia which was no longer discernible from the control value. Not surprisingly, neither carbon concentrations nor CEC values were affected by the treatments in the clay fraction.In general, the Treculia and Dactyladenia treatments were still displaying strong effects on soil organic carbon concentrations and ensuing CEC values of the total soil and its fine silt fraction in a time frame of up to 23 months after addition. Gmelina also produced similar effects, but definitely to a lesser extent. Referring to the quality of the different residues (Table 4), it becomes clear that the changes brought about in soil carbon and/or CEC are indirectly due to the differences in biochemical quality of the residues. Dactyladenia and Treculia had the lowest nitrogen contents and consequently the larger C/N ratios, predicting a slower decomposition and hence a larger residual carbon build-up. Both species also displayed the largest polyphenol concentrations, hence the largest polyphenol/N ratios, also pointing to slow decay rates. Lignin concentrations, however, were not in line with these observations.The magnitude of the changes is significant and important in view of the generally small values obtained for both organic carbon contents and CEC values in this weathered Lixisol. An increase in carbon content and charge at in situ pH in the order of 20%, still observable, 23 months after a single addition of Treculia residues is a relevant result that may lead to the inclusion of such amendments in a realistic farming system. The phenomena were -as also indicated in earlier work (Oorts et al., 2000) restricted to the fine silt fraction. Whether the absence of any effect in the clay fraction was due to a saturation of the clay fraction with organic matter or to the limited time frame (organic matter derived from the residue not yet sequestered in the clay fraction) could not be ascertained. Yet, the former possibility seems unlikely in this soil, strongly weathered and depleted in carbon. More likely seems the latter possibility confirming the slower turnover of organic components, as size of the soil particles with which they are associated becomes smaller.Both parts of the experimental program confirm the strong relation between soil organic matter and charge development in highly weathered soils, such as the ferric Lixisol in Ibadan, Nigeria. In the soil derived from the arboretum, after 20 years of continuous input of litters widely ranging in nitrogen, lignin and polyphenol contents, large differences in organic matter resulted with concomitant large differences in CEC. Because differences in CEC could be explained almost completely by the variation in soil organic carbon concentration, the effect of residue inputs was judged indirect. Differences in CEC were due to changes in the silt fractions predominantly, indicating that changes in clay fractions are not readily obtained in a time-span of less than 20 years. The decomposition tube experiment was completely in line with the above findings in this that a low quality residue proved instrumental in enhancing charge in these soils. Yet it also demonstrated that such effects could be obtained already after a single addition of 15 Mg/ha and that they were still obvious almost two years after this addition. Table 2: Organic carbon content and contribution to the whole soil CEC at pH 5.8 of the particle size fractions of the surface (0-10 cm) horizons from the selected plots in the Ibadan-arboretum.Organic carbon (g C kg -1 ) CEC (cmol c kg The fertility status of soils in the West African moist savanna is low. Two major causes are their extensive degree of weathering and the continuous mining of soil nutrients in the absence of sufficiently large amounts of external inputs or sufficiently long soil fertility-regenerating fallow periods (Jones and Wild, 1975;Smaling et al., 1997). In the absence of fertilizer additions, this low soil fertility status usually leads to very low maize grain yields on farmers' fields, e.g., around 0.75 t ha -1 in the Southern Benin Republic (Koudokpon et al., 1994), far below the potential yield of 5 -8 t ha -1 (Fisher and Palmer, 1983). As P sorption by West-African savanna soils is low compared to soils of the humid forest zone (Juo and Fox, 1977), the most limiting nutrient for cereal production in the moist savanna is generally believed to be N, followed by P.Since the early 1990s, research on natural resource management at the International Institute of Tropical Agriculture (IITA) has been following an agro-ecozonal approach. The West African moist savanna zone has been sub-divided into different agro-ecozones, each with their distinctive length of growing periods. Within each agroecozone, benchmark areas have been identified in which most of the IITA resource management research is concentrated (EPHTA, 1996). The benchmark area of the derived savanna (DS), with a growing period of 211-270 days (Jagtap, 1995), is located in Southern Benin Republic while the benchmark area of the northern guinea savanna (NGS), with a growing period of 151-180 days (Jagtap, 1995), is located in Northern Nigeria. As benchmark areas are hypothesized to contain all the biophysical and socio-economic variability found in the entire agro-ecozone, one could in principle extrapolate soil fertility management technologies developed and validated in the benchmark area to all of the agro-ecozone (EPHTA, 1996). A resource management survey implemented in the NGS benchmark led to the identification of 4 resource-use domains: a low (13.8% of survey villages), low to medium (49.2%), medium to high (23.1%), and high (13.8%) resource-use domain (Manyong et al., 1998). Resource use is quantified by an index taking into account variables describing use of external inputs, land use intensity, accessibility to markets, and diversification of the farm enterprise (Manyong et al., 1998).Besides length of growing period and the socio-economic environment, soils also vary between and within the benchmark areas. In the DS benchmark, 2 main geological units can be distinguished, giving rise to distinct soil associations. In the southern part of the benchmark the predominant soils are deep, red, kaolinitic, freely draining soils developed on coastal sediments often referred to as 'Terre de Bare' and classified as Ferralic Nitisols (FAO, ISRIC and ISSS, 1998). The northern part is underlain by crystalline basement rocks consisting mainly of granite and gneiss, which gave rise to a complex pattern of Acrisols, Lixisols, Luvisols, and Leptosols with inclusions of Vertisols and Cambisols (Faure and Volkoff, 1998). The saprolite is often found within a few meters and the clay fraction contains kaolinite and swelling (2:1) clays in varying proportions according to parent rock and drainage conditions (Volkoff, 1976a;Volkoff, 1976b). A similar resource management survey as in the NGS was implemented in the DS benchmark. This identified a set of resource use domains overlapping with the geological units (IITA, 2000). In the DS benchmark, the production of cotton is supported by a credit scheme for fertilizers and herbicides and a government-regulated market for selling the produce (Bosc and Freud, 1995).The soils in the NGS benchmark are predominantly developed in a Quaternary loess mantle which covered the Basement Complex granites, gneisses, migmatites and schists (Bennett, 1980;McTainsh, 1984). Processes of clay illuviation, iron segregation, fragmentation and horizontal transport of ironpans, and colluviation led to soil differentiation at the landscape scale. A typical toposequence consists of shallow and/or gravelly soils (Plinthosols or soils with a petroferric phase) on the interfluve crests, deeper soils (Luvisols or Lixisols) on the valley slopes and hydromorphic soils (Gleysols and Fluvisols or soils with gleyic properties) near the valley bottom (Delaure, 1998). As a common characteristic, these soils have a relatively high silt content (20-50%) reflecting the aeolian origin and have a clay fraction with low to medium activity (CEC of clay fraction between 20 and 35 cmol c kg -1 clay).Agronomically, the most straightforward measure to boost cereal grain yields is the application of fertilizers. Although it is currently believed that both fertilizer and organic matter additions are necessary to sustain agricultural production and preserve the environment (Jones and Wild, 1975;Palm et al., 1997;Vanlauwe et al., 2000b), most farmers in the moist savanna do not apply organic matter except for minimal amounts of farmyard manure and/or household waste in the NGS (Houngnandan, 2000;Manyong et al., 2000). Crop residues are commonly removed from the field either for livestock feed and other purposes in the NGS or through burning in the DS. Although average fertilizer application by farmers in the DS as well as in the NGS is low (Houngnandan, 2000;Manyong et al., 2000), the range of application rates is high. In the NGS villages, the average fertilizer N application rate was 40 kg N ha -1 with a large standard deviation of 31 kg N ha -1 (Manyong et al., 2000).The objectives of this paper were: (i) to assess the general soil fertility status of representative farmers' fields in a selected number of villages representative for the DS and NGS benchmarks, (ii) to assess the impact of soil type and fertilizer use on the selected soil characteristics, and (iii) to determine the most limiting nutrients for maize growth in the respective agro-ecozones and their relation with selected soil fertility characteristics.As the NGS benchmark area is fairly homogeneous in terms of major soils associations, the 2 villages selected in the NGS were chosen to represent the major resource use domains identified by Manyong et al. (1998). Danayamaka (7 o 50'E, 11 o 19'N) belongs to the low to medium resource-use domain and is dominated by the traditional production enterprises of the northern Guinea savanna, such as sorghum, cowpea, and livestock. Kayawa (7 o 13'E, 11 o 13'N) belongs to the medium to high resource-use domain. It is characterized by the development of new enterprises such as maize and soybean, and follows a market-oriented strategy in agricultural production (Manyong et al., 1997). The two domains together encompass 72% of the villages surveyed in the NGS benchmark area.As discussed earlier, in the DS benchmark 2 distinct geological formations are present, together covering 84% of the benchmark area (Volkoff, 1976a;Volkoff, 1976b). As the major soil characteristics were hypothesized to influence the soil fertility status of soils in this benchmark area, one village was selected belonging to each of the 2 soil associations. Zouzouvou (1°41'E, 6°53'N) lies on 'terre de barre' soils, while Eglimé (1°40'E, 7°05'N) is situated in the area underlain by crystalline rocks. The Eglimé soils are rejuvenated and much younger than the more weathered soils of Zouzouvou.A socio-economic survey on general farm characteristics and current use of fertilizer and organic inputs at the field level was implemented in the NGS (Manyong et al., 2000) and DS villages (Houngnandan, 2000). The farmers interviewed were selected following a multi-stage sampling procedure, giving a total number of 200 representative farmers in the NGS villages, and 171 in the DS villages (Houngnandan, 2000;Manyong et al., 2000). Of all fields included in the survey, 12-14 fields were randomly selected in each village to implement researcher-managed on-farm trials. In the NGS, soils near the valley-bottom or fadama soils were excluded from the selection procedure. The farmers using the selected fields were interviewed about past management of these fields. Information was obtained on cropping/fallow history and fertilizer use (type and amount) over the past 10 years.In all farmers' fields, trials were laid out containing 8 plots of 8 m by 8 m. In this paper, only the initial soil characteristics of the trials are considered; the trials themselves are the subject of forthcoming papers. Before implementation of the field trials, soil was sampled from each plot at 0-10 cm depth in April 1998 in the DS villages (one diagonal across the plots, 10 cores per plot) and in May and June 1998 in the NGS villages (both diagonals across the plots, 16 cores per plot). Afterwards, equal amounts of soil sampled from each of the 8 plots in a field were mixed to form one composite sample per field. All soil samples were air-dried and sieved to pass 4 mm. Part of the soil was ball-milled for organic C (Amato, 1983) and Kjeldahl-N analysis. A second part was analyzed for Olsen-P (Okalebo et al., 1993), effective cation exchange capacity (ECEC) (IITA, 1982), pH-water (soil:water ratio of 2.5), pH-KCl (soil:KCl solution ratio of 2.5), and texture (IITA, 1982). A third part was used to determine particle size classes of soil organic matter (SOM) by wet sieving a previously dispersed soil slurry over a nest of sieves (Vanlauwe et al., 1998). The particulate organic matter (POM) fraction consists of three separately measured SOM fractions: organic material larger than 2 mm (referred to as the 'O2000' fraction), organic material between 2 and 0.250 mm (referred to as the 'O250' fraction), and organic material between 0.250 and 0.053 mm (referred to as the 'O53' fraction).Immediately after taking the soil samples from the 0-10 cm layer, sufficient soil was taken from the same layer from between the plots to implement a missing nutrient trial, described below. The soil was air-dried and sieved to pass 4 mm before use.In the NGS villages, soil was sampled for mineral N extraction before planting maize in June 1998 (1 core in the centre of each plot bulked per field) at the following depths: 0-20 cm, 20-40 cm, 40-60 cm, and 60-80 cm. In the DS villages where a cowpea-maize rotation was implemented, soil was sampled at the same depths (2 cores per plot, bulked per field) after the cowpea harvest and before planting maize in August 1998. All samples were kept cool pending analysis. Mineral N was extracted by shaking 30 g fresh soil in 90 ml of a 2N KCl solution and filtering part of the supernatant after centrifugation of the soil slurry. The nitrate-N and ammonium-N content in the soil extract was determined colorimetrically on a continuous flow analyzer system (IITA, 1982).A missing nutrient trial with soil sampled from all individual fields was established in the greenhouse at IITA, Ibadan, Nigeria. Pots were filled with 2.5 kg of air-dried, sieved soil and the treatments presented in Table 1 were implemented. After applying 75 ml of nutrient solution per pot, an additional 340 ml of distilled water was applied just before planting. Although the nutrient solutions were composed such that only the nutrients under consideration were missing -except for the 'all-N' treatment where Clwas added -the final pH (varied between 3.3 and 6.1) and electrical conductivity (varied between 0.9 and 2.4 dS m -1 ) of the solutions were not equal. Preliminary testing, however, showed that after mixing a selected number of soils with the nutrient solutions, final soil pH values were hardly affected due to the buffering capacities of the soils (maximal differences in pH after applying the various nutrient solutions was 0.18 pH units for the selected soils). For the 'minus-N' treatment, a selected number of pots was also included with CaCO 3 as the Ca source rather than CaCl 2 to assess whether the addition of Clhad an effect on plant growth. As both Ca sources gave similar maize growth (data not shown), it was concluded that the addition of Cldid not affect maize growth. Although the differences in electrical conductivity of the nutrient solutions are the only factors besides the missing nutrients considered which could influence maize growth, the total salt concentrations were low (varied between 0.2 and 0.4 dS m -1 after applying the nutrient solutions to the soil as measured in a 1:2.5 soil:water suspension at 25°C) and as such, this factor was presumed not to influence maize growth.The pots were arranged in a randomized complete block design with 3 replicates. After application of the nutrient solutions and distilled water, 4 maize seeds (variety Oba Super 2) were planted in each pot and thinned to 2 plants per pot after germination. The pots were watered twice daily thereby avoiding leakage of water through the bottom of the planting pots and avoiding signs of moisture stress on the maize plants. After 7 weeks, the maize plants were cut at the soil surface, oven-dried (65°C), and weighed. The roots were extracted from the soil by sieving over a 0.5 mm sieve, washed, oven-dried, and weighed.In the pot trial, the relative biomass production in the treatments with one or a range of nutrients removed vs the treatment with complete nutrition was calculated as (equation 1):Maize shoot or root biomass in the treatment with one or more missing nutrients ⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯⎯ * 100 (1)Maize shoot or root biomass in the treatment with all nutrients applied According to equation ( 1), a higher relative yield indicates a lower response to the missing nutrient considered.The land use management, soil, and maize data were analyzed with the MIXED procedure of the SAS system (SAS, 1992) using 'benchmark' and 'village within benchmark' as fixed variables and 'field*village within benchmark' as a random factor. Significantly different means were separated with the PDIFF option of the LSMEANS statement.To assess the impact of fertilizer use on the observed soil characteristics, the data were also analyzed using 'benchmark' and 'fertilizer class' as fixed variables and 'field*fertilizer class within benchmark' as a random factor. The 'fertilizer class' of a certain field was obtained by rounding the average of the 'N fertilizer class' and the 'P fertilizer class' values for that field. Three 'N fertilizer classes' were defined: I: > 60 kg N ha -1 yr -1 ; II: 30-60 kg N ha -1 yr -1 , III: < 30 kg N ha -1 yr -1 and 3 'P fertilizer classes' I: > 20 kg P ha -1 yr -1 ; II: 10-20 kg P ha -1 yr -1 , III: < 10 kg P ha -1 yr -1 (Fig. 1). As fertilizer class was counfounded within village in the DS villages -nearly all the Zouzouvou soils belong to the class with the lowest fertilizer use, while nearly all Eglimé soils belong to the class with the highest fertilizer use (Fig. 1) -it was not possible to include both 'village' and 'fertilizer class' in the same ANOVA as certain factors were not estimable. Regression analysis was used to calculate relationships between response to N and P and soil nutrient contents.Fallows are still quite common in Zouzouvou (2.3 yrs per 10 yr) and virtually non-existent in all other villages (Table 2). In both benchmark areas, cereals are important crops, while more legume crops were grown during the past 10 yr in the DS villages. In the DS, cotton is a common cash crop, and in the NGS pepper and tomato are commonly grown. The type of NPK fertilizer commonly used is different in the two benchmarks. In the DS, cotton fertilizer (14%N, 23%P, 14%K, 5%S, 1%B) is virtually the only compound fertilizer available, and in the NGS several blends can be found, but 15:15:15 and 20:10:10 are the most common ones (Table 2).While yearly N fertilizer application rates were not significantly different between the 2 benchmark areas, large differences in fertilizer use between the 2 villages in the DS benchmark were observed (Table 2). Farmers in Eglimé used, on average, 88 kg N ha -1 yr -1 for the past 10 years, and farmers in Zouzouvou used less than 10 kg N ha -1 yr -1 . Differences in fertilizer use between the 2 villages in the NGS were not significant. Farmers in the DS villages used significantly more P fertilizer (27 kg P ha -1 yr -1 ) than farmers in the NGS villages (17 kg P ha -1 yr -1 ), but again, striking differences in P use were observed between Zouzouvou (8 kg P ha -1 yr -1 ) and Eglimé (45 kg P ha -1 yr -1 ). In the DS, proportionally more N fertilizer was applied as urea in Eglimé than in Zouzouvou, and the same was true for Danayamaka in the NGS (Table 2).While topsoils in the DS benchmark were generally more sandy and less silty than soils in the NGS benchmark, their organic C, total N, Olsen-P, exchangeable Ca and Mg contents and ECEC were significantly higher (Table 3). Soils in the DS contained more SOM particles with a higher particle size and soils in the NGS contained significantly more of the 'O53' material, leading to similar POM contents in both benchmarks (Table 3).Although the soil chemical and organic matter characteristics of the 0-10 cm layer appeared to have larger values in Kayawa than in Danayamaka, none of the differences were significant, except for the O53 and POM content (Table 3). This is in sharp contrast with the DS villages, where topsoil in Eglimé contained a significantly larger amount of C, N, Olsen-P, exchangeable Ca, Mg, and K, and had a significantly higher ECEC. Topsoils in Eglimé also had a significantly lower sand and a significantly higher silt content than topsoils in Zouzouvou (Table 3). In the DS villages, nitrate-N and ammonium-N contents were similar for all soil depths, except for the 60-80 cm layers in which fields in Eglimé had significantly more ammonium-N than fields in Zouzouvou (Fig. 2a). The ammonium-N content in the 0-20 cm and 20-40 cm layers was significantly higher in Kayawa than in Danayamaka, while differences in nitrate-N content were similar at all soil depths (Fig. 2b). The soil profile contained more ammonium-N than nitrate-N in all soil layers and villages.In both the DS and NGS villages, differences in soil organic C and total N content between fertilizer classes were not significant (Table 4). Olsen-P and exchangeable K contents, on the other hand, were significantly higher in class I than in class II or class III soils in both agro-ecozones. In the DS villages, exchangeable Ca and Mg contents were significantly higher in class I than in class III soils (Table 4). No significant differences in mineral N content of the soil profile were found between the different fertilizer classes (data not shown).The relative biomass yield of maize shoots in absence of N was similar for both benchmarks (Table 5). Soils in the NGS showed a lower relative shoot biomass yield in the absence of P than soils in the DS, indicating a stronger response to applied P (Table 5). In both benchmarks, responses to cations, S, and micronutrients were negligible. In the DS, maize shoot biomass responded more strongly to P in Zouzouvou than in Eglimé, while responses to N were similar in both villages. In the NGS, no differences in responses to N and P between villages were observed (Table 5).Although a significant linear relationship was observed between shoot biomass response to N and the soil total N content, the relationship explained only 36% of the overall variation (Fig. 3a). This value decreases to 18% (significant at the 1% level) if the data point lying outside the cloud of points is omitted from the regression analysis. The relationships between shoot biomass response to P and Olsen-P contents followed a linear pattern up to about 12 mg Olsen-P kg -1 , after which responses tended to reach a plateau (Fig. 3b). The linear relationships between shoot biomass response to P and Olsen-P contents below 12 mg Olsen-P kg -1 explained between 60% and 74% of the overall variation for Zouzouvou, Danayamaka, and Kayawa. Slopes nor intercepts of the linear relationships were significantly different between these villages. Most of the Eglimé soils contained amounts of Olsen-P exceeding 12 mg P kg -1 and responses to P were part of the plateau of the relationship (Fig. 3b).The relative maize shoot biomass yield in absence of P was significantly smaller for soils from the NGS (relative yield of 33%) than from soils from the DS (67%), and within the DS, for soils from Zouzouvou (58%) than for soils from Eglimé (75%). The favourable P status in Eglimé soils is certainly caused by the extensive use of P-containing 'cotton' fertilizer, stimulated by the government-supported credit and marketing schemes for cotton production in Benin Republic. This observation is a good example of how agricultural policies may influence soil fertility status. Although the same policies apply to Zouzouvou, the lower fertilizer use in Zouzouvou compared to Eglimé may be explained by the lower occurrence of cotton (data not shown) and the lower inherent fertility of the soils caused by more intense weathering. Although 'cotton' fertilizer is usually applied to cotton, P fertilizer is known to have considerable residual effects on soils with low P sorption capacities (Bationo et al., 1986;Buresh et al., 1997), allowing other crops grown in rotation with cotton to benefit from this added P. Although use of P fertilizer is much lower in Zouzouvou than in Eglimé and comparable to P use in the NGS villages, the relative shoot biomass yield in absence of P is higher in Zouzouvou than in the latter villages. This may be related to differences in P sorption capacities of the A and Bt soil horizon between the soil profiles (Nwoke et al., unpublished data). The higher P sorption of the NGS soils is most likely related to the greater amount of fine particles and the composition of these fine particles (Mokwunye et al., 1986). On the other hand, adulteration of locally produced fertilizer blends in the NGS can not be excluded and may have led to lower P application rates as calculated from the information given by the farmers.The Olsen-P content appears to be a good indicator for P availability of West African moist savanna soils (Fig. 3b), as previously reported by Vanlauwe et al. (2000a). Soils containing Olsen-P values over 12 mg kg -1 are less likely to respond to applied P than soils with Olsen-P values below 12 mg kg -1 . For the latter soils, the response to P increased linearly with decreasing P content. Due to the high fertilizer application rates in Eglimé, little or no response to added P was observed for these soils. As 'cotton' fertilizer is the only commonly available compound fertilizer in Benin, one could wonder whether the composition of this fertilizer is agronomically and economically optimal for application to maize as maize is known to require relatively higher amounts of N than P (Wichmann, 1998).Notwithstanding significant differences in soil organic C and total N content between benchmarks and, in the DS, between villages, responses to applied N were similar in all villages and benchmarks. Moreover, only a minor fraction of the total variation was explained by a linear relationships between response to N and soil total N content. These observations indicate that total C and N are weak indicators of potential soil N supply. Although inputs of organic matter are expected to be larger in the DS than in the NGS because of the longer growing period, fallow and crop residues are commonly burnt in the DS villages before planting the first season crop. In the NGS, fallow vegetation at the start of the cropping seasons is minimal and crop residues are commonly removed from the field for livestock feed, fencing, or other purposes. As belowground plant components and weeds are the major organic matter inputs, differences between benchmarks may not be as large as would be expected taking into account only the length of growing periods. This is also confirmed by the similar amounts of the easily available POM pool, which was shown to be rather easily influenced by application of fresh organic matter (Vanlauwe et al., 1999). As inputs of organic matter are expected to be in the same order of magnitude in both benchmarks, the larger C contents in the DS soils, and especially in Eglimé, indicate that in the DS, a higher proportion of the C is either physically and/or chemically protected from mineralization. This may be related to the more frequent burning of crop residues and consequent chemical stabilisation of C as charcoal in the DS villages. Physical protection of soil organic C is expected to be higher in the soils of the NGS villages due to their higher silt content and associated C protection capacity (Hassink and Whitmore, 1997). However, commonly used soil tillage practises may hamper the soil C protection mechanisms in contrast with the DS villages, where soils are usually only minimally tilled during weeding activities. Although the input of organic matter as above and belowground crop residues is expected to be larger in Eglimé than in Zouzouvou because of the much higher fertilizer application rates (Table 2), the frequent burning of crop residues and the higher silt content and associated C protection capacity (Hassink and Whitmore, 1997) in the Eglimé soils may mask the potentially higher N supply capacities of these soils. This is also obvious from the similar amounts of mineral N in the soil profile before maize planting. The larger amounts of mineral N in the topsoil in Kayawa compared with Danayamaka are likely the results of a larger amount of easily decomposable POM (Table 3).The response to missing cations, S, and micronutrients was virtually nil in all fields, indicating that these nutrients are not an immediate source of concern. However, applying higher rates of N and P fertilizer may more rapidly exhaust the soil reserves of these nutrients and lead to other major deficiencies. Especially 'terre de barre' soils, which have an inherently low available K content, as confirmed by the data presented in Table 3, may be susceptible to K deficiency when agricultural production increases (Jones and Wild, 1975). On the other hand, as long as local fertilizer recommendation schemes include application of K fertilizer (Carsky and Iwuafor, 1999) and as farmers usually apply NPK fertilizers, this possibility may turn out to be a rather theoretical one.Certain differences in soil characteristics between villages within one benchmark area are surely rather the result of inherent soil type characteristics than of management practices. The higher base status and silt content of the Eglimé soils compared with the Zouzouvou soils, e.g., is related to the higher base content of the parent material rather than to the use of external inputs. After all, the Eglimé soils are rejuvenated and much younger than the more weathered soils of Zouzouvou. The high silt content of the soils in the NGS villages reflects the aeolian origin of the parent material, formed by deposition of loesslike material by Harmattan winds (Bennett, 1980). Through this dust deposition, the soils in the NGS are enriched with bases at an annual rate of 19 kg K ha -1 , 10 kg Ca ha -1 , and 4 kg Mg ha -1 (McTainsh, 1982). Dust deposition decreases from North to South, and annual enrichment rates in the DS are of the order of 3 kg K ha -1 , 5 kg Ca ha -1 , and 2 kg Mg ha -1 (Hermann, 1996, cited by Stahr et al., 1996). Other differences in soil characteristics are more likely brought about by differences in soil management and particularly fertilizer use. Although one could argue that in the DS the larger P and K content of soils belonging fertilizer class I compared with soils belonging to fertilizer classes II and III is caused by the fact that fertilizer classes and soil type are confounded (most Eglimé soils belong to class I, while most Zouzouvou soils belong to class III), similar observations were made for fertilizer classes in the NGS, where soil types are similar (Table 4). This clearly shows that application of external sources of P and even K can improve the general P and K status and benefit future crops. This is not true in the case of N fertilizers, as neither the soil total N content nor the mineral N content in the soil profile varied between fertilizer classes (Table 4). One consequence of this observation is that N fertilizers need to be applied yearly to sustain crop growth. While it is often claimed that excessive long-term use of N fertilizers may decrease the soil pH, it is worth noting that topsoil pH values are similar for Zouzouvou and Eglimé (Table 4), while the difference in average yearly N fertilizer application is substantial (Table 2). This indicates that the acidifying activity is not relevant for all fertilizers. (Juo et al., 1995) already found that the acidifying effect of N fertilizer was highest for ammonium sulphate, lower for urea and virtually absent for calciumammonium-nitrate.Palm CA, Myers RJK and Nandwa SM (1997) a All pots received 75 ml of the respective nutrient solutions and 340 ml of distilled water at planting. a Fertilizer classes for N application are: I: > 60 kg N ha -1 yr -1 ; II: 30-60 kg N ha -1 yr -1 , III: < 30 kg N ha -1 yr -1 . Fertilizer classes for P application are: I: > 20 kg P ha -1 yr -1 ; II: 10-20 kg P ha -1 yr -1 , III: < 10 kg P ha -1 yr -1 . Overall fertilizer classes, used in the column headings of this Several of the unresolved questions related to alley cropping in particular and agroforestry systems in general are associated with the root dynamics of the tree component. In alley cropping systems, 'ideal' hedgerows recover soil N and other nutrients only from layers below the rooting depth of the accompanying food crop. By doing so, trees recover nutrients leached beyond the reach of annual food crops and thus improve nutrient use efficiencies. Real-world hedgerows recover a substantial proportion of their nutrients from layers simultaneously exploited by food crop roots and increase competition in favor of the trees. To assess possible belowground competition for water and nutrients between the trees and associated food crops, data on root abundance as a function of soil depth, soil characteristics, and time are needed (Schroth, 1995;Van Noordwijk and Purnomosidhi, 1995).Senna siamea Irwin & Barneby is a non-N 2 -fixing leguminous tree which is commonly found in natural fallows in the moist savanna zone of West-Africa. Senna has been widely used in alley cropping trials (Ruhigwa et al., 1992;Danso and Morgan, 1993;Van der Meersch et al., 1993;Schroth and Lehmann, 1995;Aihou et al., 1999;Tossah et al., 1999;Vanlauwe et al., 2001a) or other agroforestry systems (Leihner et al., 1996) in West-Africa. Tossah et al. (1999) reported annual Senna aboveground biomass productions of 9.2, 1.8, and 9.8 ton ha -1 , in Glidji (Southern Togo) on a Rhodic Ferralsol, in Amoutchou (Central Togo) on a Haplic Arenosol, and in Sarakawa (Northern Togo) on a Ferric Acrisol, respectively. Although Senna has been depicted as an aggressive scavenger for nutrients due to its laterally spreading root system (Hauser, 1993), Aihou et al. (1999) and Tossah et al. (1999) concluded that Senna trees rely mainly on the subsoil as a source of nutrients. While Vanlauwe et al. (2001a) found only a small recovery of applied 15 N-urea in the Senna hedgerow during intercropping with maize on a non-acid Alfisol, Ruhigwa et al. (1992) concluded that Senna would compete for nutrients with the associated food crop in alley cropping systems, as most of its fine root biomass was confined to the top 20 cm of an acid Ultisol. Schroth et al. (1995) stated that the lateral development of Senna roots was favoured by the shallow soil depth on a Ferric Acrisol in Central Togo. Akinnifesi et al. (1995) found significant decreases in root length densities of Enterolobium cyclocarpum and Leucaena leucocephala with increases in soil bulk density. Above observations clearly indicate possible interactions between soil chemical and physical conditions on the one hand and the root distribution and competitive character of Senna trees on the other hand.The objectives of this paper were (i) to quantify the root distribution of Senna hedgerows, growing in alley cropping systems on a number of sites representative for the derived savanna of Togo, (ii) to evaluate the effect of soil profile characteristics on the observed root distributions, (iii) to assess the potential of Senna trees to recover nutrients leached beyond the reach of food crops or the so-called safety-net efficiency, and (iv) to explore relationships between the different methods used to quantify root distributions.The trials were established on a Rhodic Ferralsol in Glidji (Southern Togo -6°15'N, 1°36'E), on a Haplic Arenosol in Amoutchou (Central Togo -7°22'N, 1°10'E), and on a Ferric Acrisol in Sarakawa (Northern Togo -9°37'N, 1°01'E). The present soil types represent about 57% of the soils in the DS (Jagtap, 1995). All sites are located in the Derived Savanna (DS) zone, which is characterized by a length of growing period between 211 and 270 days (Jagtap, 1995). Total rainfall in Glidji was 950 mm in 1995 and 876 mm in 1996 (bimodal pattern), in Amoutchou 1540 mm in 1995 and 1250 mm in 1996 (unimodal pattern), and in Sarakawa 1357 mm in 1995 and 1289 mm in 1996 (unimodal pattern). The site in Amoutchou had a groundwater table between 0.8 and 1.4 m below the soil surface, while the groundwater table of the others sites is deeper than 10 m.The trials were established in 1991 in Glidji and in 1992 in Amoutchou and Sarakawa. A randomized complete block design with four replicates was laid out with five treatments consisting of four alley cropping plots and a no-tree control treatment. Plot size was 10 by 12 m and the hedges were planted at 4 m distance, making 3 10-m-long hedges per plot. The Senna seeds used in the three sites were collected from a Senna fallow near Lomé, Togo. In Glidji, the Senna trees were pruned 3 times yearly (before planting around mid-April, about 5 weeks after planting, and about 11 weeks after planting) in 1992 and again in 1994, 1995, and 1996 at 0.25 m above the soil surface while in Amoutchou and Sarakawa, the Senna trees were pruned 3 times in 1995 and 1996 (Tossah et al., 1999). During the years in which the trees were pruned, maize was planted at a distance of 80 (between rows) by 30 cm (within rows) and thinned to one plant per pocket. A basal application of 26 kg P ha -1 as TSP and 50 kg K ha -1 as KCl was applied to the maize at planting follwed by two applications of 22.5 kg N ha -1 of urea approximately 3.5 and 7.5 weeks after planting.In September 1996, a trench was dug in each field in two Senna alley cropping plots, perpendicular to the hedgerow, 15 cm away from the tree base, extending 2 m away from the trees, and 2 m deep. After leveling the profile wall, Senna root abundance was determined using a 10 by 10 cm grid by counting all living roots within each grid, after removing the top 1 mm of soil, following the method described by Akinnifesi et al. (1999). Dead roots were identified by their brittle structure and dark cortex. Roots > 5 mm, between 2 and 5 mm, and < 2 mm were counted separately.After determining the root abundance, soil samples were taken from the profile wall with a 10 by 10 cm square auger (5 cm deep), 0.1, 0.5 and 1.5 m away from the tree base, from the following soil layers : 0-10, 10-20, 20-30, 50-60, 80-90, 110-120, and 140-150 cm. In Amoutchou, both root counting and soil sampling was restricted to 100 cm because of the high water table. In Glidji, soil was also destructively sampled at 190-200 cm. Separate soil samples were taken from the same layers for routine soil analysis (organic C (Amato, 1993); Kjeldahl total N; effective cation exchange capacity (IITA, 1982); base saturation; pH(H 2 0) (20 g dry soil in 50 ml H 2 O); texture (IITA, 1982)). The diameter of the taproot was measured at 10 cm depth intervals up to a depth of 2 m, after taking the soils samples for root and soil characterization. Bulk densities were determined on the wall of a nearby soil profile, dug in 1995 to determine the soil type (Tossah et al., 1999).The roots were removed from the soil collected with the 10 cm by 10 cm square augers by washing over a 0.5 mm sieve after submerging the samples overnight in a hexametaphosphate-Nacarbonate solution (20.94 g Na-hexametaphosphate L -1 and 4.45 g Na 2 CO 3 L -1 ) and stored in a 1% formaldehyde solution. Dead and live roots were separated as described above. The roots < 2 mm were spread evenly on a perspex sheet, scanned with Paintshop Program software, and the root length density (RLD) were measured with the Delta-T-Scan image analysis program (Webb et al., 1993). Preliminary investigations with a limited number of root samples showed a very close relationship between root lengths measured with the image analysis program and the original Tennant-method (Tennant, 1975).All root data were subjected to ANOVA with the MIXED procedure of the SAS system (Littell et al., 1996). Regression analysis between the various root characteristics was carried out with the REG procedure of the SAS system (SAS, 1985). Tap root diameters, root weight densities, and root length densities were log-transformed before ANOVA (Gomez and Gomez, 1984). Large (> 2mm) and small (< 2mm) root abundances, collected on the profile wall, were combined into 4 distances (0-50, 50-100, 100-150, and 150-200 cm away from the tree) and 6 depths (0-20, 20-40, 40-80, 80-120, 120-160, and 160-200 cm) for ANOVA analysis. Values for root abundance were log(n+1) transformed before statistical analysis (Gomez and Gomez, 1984). Two extremely large values for root length density measured on one of the two Glidji profiles (see below) were excluded from the statistical analysis. Means were estimated with the LSMEANS statement, while significantly different means were separated with the PDIFF test of the LSMEANS statement (Littell et al., 1996).The profile in Amoutchou contained mostly sand down to 1 m depth, while the profiles in Glidji and Sarakawa showed clay accumulation below 50 cm (Table 1). Consequently, the organic C and total N content and ECEC are higher in the subsoil in Glidji and Sarakawa than in Amoutchou. While the bulk density of the topsoil was similar in all sites, the bulk density of the layers below 40 cm was lower in Amoutchou than in both other sites (Table 2).For all sites, the abundance of roots < 2 mm diameter in the 0-20 cm, 40-80 cm, and 80-120 cm layers was significantly (P < 0.05) higher between 0 and 0.5 m away from the hedgerow (21.4, 5.3, and 204 5.5 dm -2 in the 0-20 cm layer in Glidji, Amoutchou, and Sarakawa, respectively) than between 1.5 and 2 m away from the hedgerow (13.8, 3.1, and 3.7 dm -2 in the 0-20 cm layer in Glidji, Amoutchou, and Sarakawa, respectively) (Fig. 1). This was also true for the 20-40 cm layer in Amoutchou, for the 120-160 cm layer in Glidji and Sarakawa, and for the 160-200 cm layer in Sarakawa. Distance to hedgerow had no effect on the number of roots < 2 mm in the 20-40 cm layer in Glidji and Sarakawa (Fig. 1). In Glidji and Sarakawa, more shallow soil layers contained significantly (P < 0.05) more roots < 2 mm than deeper soil layers up to 120 cm depth at the 4 considered distances away from the tree base. Below 120 cm, differences between layers were not consistently significant. In Amoutchou, only the top 0-20 cm layer contained more (P < 0.05) roots < 2 mm than the deeper soil layers, while below 20 cm differences in root abundance were not consistently significant (Fig. 1).In Glidji and Sarakawa, the abundance of roots > 2 mm in the 0-20 cm layer was significantly (P < 0.05) higher close to the hedgerow (0-0.5 m) (1.8 and 0.8 dm -2 in Glidji and Sarakawa, respectively) than furthest away from the hedgerow (1.5-2 m) (0.8 and 0.4 dm -2 in Glidji and Sarakawa, respectively) (Figs. 2a and 2c). Below 80 cm no differences in number of roots > 2 mm were observed for the considered lateral distances. In Amoutchou, all soil layers contained more (P < 0.05) roots > 2 mm closest to the hedgerow (0-0.5m) (1.1 dm -2 in the 0-20 cm layer) than furthest away from the hedgerow (1.5-2 m) (0.3 dm -2 in the 0-20 cm layer) except the 20-40 cm layer (Fig. 2b). Generally, in Glidji and Sarakawa, more shallow (0-80 cm) soil layers contained more (P < 0.05) roots > 2 mm, while in Amoutchou, only the 0-20 cm layer contained more (P < 0.05) large roots than the layers below 20 cm. Below 80 cm, no differences in large root abundance were observed between soil layers.The root length density (RLD) of the 0-10 cm and 10-20 cm layers was significantly (P < 0.05) higher in Glidji (1.46 and 1.15 cm cm -3 in 0-10 and 10-20 cm layer, respectively) than in Amoutchou (0.50 and 0.22 cm cm -3 in 0-10 and 10-20 cm layer, respectively) (Fig. 3a). The 10-20 cm and 20-30 cm layers had a significantly (P < 0.05) higher RLD in Sarakawa than in Amoutchou. Differences in RLD between sites were not significant for layers below 30 cm (Fig. 3a). The 0-10 cm layer had a higher (P = 0.08) RLD under the tree than 1.5 m away from the tree (Fig. 3b). Deeper layers contained similar root length densities (RLD's) irrespective of the distance to the tree base (Fig. 3b). In Glidji, 2 soil cores (0-10 cm and 10-20 cm, both 0.5m away from the tree) in one of the profile pits contained very high RLD's (22.8 and 24.9 cm cm -3 , respectively), which were excluded from the statistical analysis, as mentioned previously.The root weight density (RWD) of the 0-10 cm was similar in all sites (0.64, 0.44, and 0.56 mg cm -3 in Glidji, Amoutchou, and Sarakawa, respectively) (Fig. 4a). The 10-20 cm layer had a significantly (P < 0.05) higher RWD in Glidji than in Amoutchou. Differences in RWD between sites were not significant for layers below 20 cm (Fig. 4a). The 0-10 cm layer had a higher (P = 0.09) RWD under the tree than 1.5 m away from the tree (Fig. 4b). Deeper layers contained similar root length densities (RWD's) irrespective of the distance to the tree base (Fig. 4b).At the soil surface, the taproot diameter was significantly (P < 0.05) larger in Glidji (215 mm) than in Amoutchou (91 mm) and in Sarakawa (77 mm) (Fig. 5). Between 10 and 50 cm, no significant differences in taproot diameter between sites were observed. Between 60 and 100 cm, the taproot diameter was significantly (P < 0.05) larger in Amoutchou than in Sarakawa (Fig. 5). For all sites, the taproot diameter decreased with soil depth, although differences between specific soil layers were not consistently significant (Fig. 5).For all sites, significant (P < 0.001) linear regressions were observed between RLD's, RWD's, and abundances of roots < 2 mm (Fig. 6). While the slopes of the regression lines relating RLD's with numbers of small roots were similar for all sites (Fig. 6a), the slope of the regression line relating RWD's with numbers of small roots was significantly higher for Amoutchou than for Glidji (Fig. 6b). The regression line relating RLD's with RWD's had a significantly higher slope for the Glidji data than for the data obtained on the other two sites (Fig. 6c).In Amoutchou, the relative RLD, calculated based upon the regression lines presented in Fig. 6a, appeared to be lower than in Glidji or Sarakawa for the top 40 cm of soil, while the inverse was observed for the layers between 50 and 100 cm deep (Fig. 7).The soil profile characteristics influenced root abundance in the different soil layers. Although none of the soil layers in the top 1 m showed severe chemical (Table 1) or physical (Tables 1 and 2) restrictions to root growth, the soil layers below 50 cm contained a relatively higher RLD in Amoutchou than in the two other sites, most likely because of their more sandy texture (Table 1) and lower bulk density (Table 2). The taproot diameter in the layers below 50 cm was also larger in Amoutchou than in Sarakawa. The presence of local accumulations of roots observed in the Glidji topsoil and local increases in root abundances in the subsoil (Fig. 1) indicates that roots are not homogeneously distributed within a certain soil layer, but follow trails with minimal resistance to root growth, such as macropores or soil cracks. Rowe et al. (1999) reported a large variation in recoveries of subsoil 15 N-labeled ammonium sulphate by Peltophorum dasyrrhachis and attributed this to large heterogeneity in root distributions.The larger values for root abundance in the topsoil in Glidji compared to Sarakawa was most likely caused by the more intense pruning regime, as the chemical and physical characteristics of the topsoil varied only little between the two sites (Tables 1 and 2). After all, in Glidji, the trees were pruned the first time already one year after planting and had been pruned 12 times prior to root quantification, while in Amoutchou and Sarakawa, the hedges grew for 4 years before their first pruning and had been pruned only 6 times before root quantification. Van Noordwijk and Purnomosidhi (1995) observed that a lower pruning height led to a larger number of superficial roots of smaller diameter on an Indonesian Ultisol. Schroth (1995) stated that shoot pruning of trees seemed to increase root branching in the topsoil and restrict tree roots to shallower soil depths compared with roots of unpruned trees. Although in Glidji also root length densities in the top 20 cm layer were much higher than in the two other sites, root weight densities were similar in all sites. This could be an indication that a more intensive pruning regime does not only lead to a larger number of superficial roots but also to roots with a smaller diameter. The necessity to prune the hedgerow trees in alley cropping systems during the cropping season results in a tree root system more comparable to the root system of an annual crop and, as such, reduces the potential of hedgerows to fulfill their hypothesized nutrient recovery potential. As regular pruning affects both the distribution of roots in the profile and their size, one could argue that screening of hedgerow trees for root competitiveness should be done on regularly-pruned trees and not on trees that are allowed to grow continuously.The root safety-net zone is usually equated with that part of the soil profile from where trees recover substantial amounts of nutrients, not accessible to the associated food crop. Cadisch et al. (1997) developed an index for quantifying the nutrient recovery efficiency of the root safety-net -the safety-net efficiency -defined as the ratio [tree N uptake from the safety-net layer]:[tree N uptake from the safetynet layer + N leached beneath the safety-net layer]. A high safety-net efficiency requires a minimal RLD to a certain depth, a minimal level of activity of the roots present in the soil layers considered, and a minimal demand by the tree for the nutrient considered. Assuming that during the major part of the maize growing season few maize roots are found below 60 cm (Vanlauwe et al., 2001b), Senna root safety-nets could be identified in Glidji and Sarakawa with a thickness of at least 140 cm and minimal RLD's of 0.2 and 0.1 cm cm -3 , respectively. In Amoutchou, a Senna root safety net could be identified with a thickness reaching the upper boundary of the ground water table and a minimal RLD of 0.1 cm cm -3 . The safety-net was also observed to cover the complete alley from hedgerow to hedgerow, as the distance to hedgerow had only an impact on RLD's for the 0-10 cm soil layer, maximally 50 cm away from the tree base. The minimal RLD's needed for maximal nutrient uptake depend on the anion, but the safety-net hypothesis is usually linked to the recovery of nitrate-N as this nutrient is very mobile. Van Noordwijk (1989) estimated the minimal RLD to be 0.1 cm cm -3 for nitrate recovery and 1 cm cm -3 for K recovery. Although based on the observed RLD's the trees growing in all sites have the potential to recover a substantial amount of mineral N from the subsoil, some important processes and tree management aspects may hamper the optimal functioning of the root safety-net. Firstly, mineral N dissolved in water flowing preferentially through macropores may bypass any recovery mechanisms of mineral N by the trees. Vanlauwe et al. (2001a) observed substantial amounts of urea-derived N in the 120-150 cm soil layer already at 21 days after urea application and attributed this to preferential flow through macropores. Although tree roots may equally prefer to grow through macropores, it is doubtful whether water moving down macropores can be sufficiently fast absorbed by tree roots growing through these macropores. Secondly, the presence of roots in the subsoil does not necessarily mean that they are actively retrieving nutrients from the soil solution, although Schroth (1995) stated that the presence of roots from competitive crops such as maize may restrict the lateral spread of tree roots and force them into the subsoil. Vanlauwe et al. (2001a) also observed a larger recovery of 15 N-labeled ammonium sulphate by the maize than by the Senna hedgerow in an alley cropping trial. Evidently, during the dry season, trees will rely mostly on their subsoil roots for nutrient and water uptake. Thirdly, pruning of the tree canopy at the start of the food crop growing season strongly restricts the demand of the hedgerow for nutrients and water at a time where nutrient availability may be high due to the application of prunings and/or fertilizer and due to the presence of relatively large amounts of mineral N after the first rains caused by the socalled 'Birch' effect.Although most of the soil layers in the Glidji profile contained a larger RLD than in the Sarakawa profile, especially in the top 20 cm, the average yearly pruning biomass productions was similar on both sites (9.2 and 9.7 t ha -1 in Glidji and Sarakawa, respectively - Tossah et al., 1999). The impact of a more dense root systems in Glidji is likely to be counteracted by the lower yearly precipitation, a lower top and subsoil fertility status (Table 1), and a higher competition with maize due to a relatively higher proliferation of tree roots in the same soil layers with maximal maize root densities. The very low yearly biomass production in Amoutchou (1.8 t ha -1 - Tossah et al., 1999) is likely caused by the very low soil fertility status of the complete profile and the temporarily high groundwater table which restricts nutrient uptake to the top 1 m during the rainy season.The highly significant relationships between RLD's and RWD's and their relatively high R² values indicate that both root characteristics are closely related, irrespective of sampling depth or distance to hedgerow tree. For similar RWD's, Senna roots in Glidji had a significantly higher RLD, which confirms that they had a smaller diameter in Glidji than in the other two sites, as discussed earlier.Although the linear regressions between RLD's or RWD's and the number of small roots counted on a profile wall were highly significant, these regressions explained less of the variation than regressions between RLD's and RWD's. This may not be surprising as the ratio [RLD in a three-dimensional volume]:[number of roots visible on a two-dimensional plane] depends on the spatial arrangement of the tree roots and varies with sample position, sample depth, and sampling time (Van Noordwijk, 1987). As the relationships between RLD and small root abundance are quite similar for all sites, these could be used to estimate RLD's from root counting data on a profile wall, provided the relationship between the various root characteristics is known for the species of interest.The sandy profile in Amoutchou resulted in a relatively higher proportion of RLD's in the subsoil and a larger tap root diameter compared to the Glidji and Sarakawa, of which the soil profile contained a clay accumulation horizon. The Senna roots contained more roots of a smaller diameter in Glidji than in Sarakawa, which was most likely the result of differences in tree management rather than soil profile characteristics.In Glidji and Sarakawa, root safety-nets with a thickness of at least 140 cm and a minimal RLD of 0.2 and 0.1 cm cm -3 , respectively, were present. In Amoutchou, the thickness was limited due to the presence of a temporarily high groundwater table. However, the presence of tree roots at a certain depth does not prove that they are active. Moreover, several processes and tree management practices were identified which may lead to significant bypasses of the safety-net.Close relationships were found between RLD's and RWD's indicating that RLD's could be estimated by a less tedious quantification of RWD's. Although the linear regressions between RLD's or RWD's and the number of small roots counted on a profile wall were highly significant, these regressions explained less of the variation than regressions between RLD's and RWD's. Fig. 1: Abundance of Senna siamea roots with a diameter < 2 mm in Glidji (a), Amoutchou (b), and Sarakawa (c) in Togo, West Africa, as influenced by soil depth and distance to the tree base. Values are averaged over the two halves of the two profile pits. Minimal and maximal standard errors of the differences between log(n+1)-transformed data are 0.044 and 0.062, 0.045 and 0.069, and 0.039 and 0.055, for Glidji, Amoutchou, and Sarakawa, respectively. Note that in Amoutchou no observations were taken below 100 cm. Fig. 2: Abundance of Senna siamea roots with a diameter > 2 mm in Glidji (a), Amoutchou (b), and Sarakawa (c) in Togo, West Africa, as influenced by soil depth and distance to the tree base. Values are averaged over the two halves of the two profile pits. Minimal and maximal standard errors of the differences between log(n+1)-transformed data are 0.020 and 0.028, 0.028 and 0.044, and 0.018 and 0.026, for Glidji, Amoutchou, and Sarakawa, respectively. Note that in Amoutchou no observations were taken below 100 cm. Minimal and maximal standard errors of the differences between log-transformed data are 0.17 and 0.25 for Fig. 3a and 0.15 and 0.23 for Fig. 3b. The interaction between site, soil depth, and distance to tree base was not significant. Note that in Amoutchou no observations were taken below 100 cm. Minimal and maximal standard errors of the differences between log-transformed data are 0.27 and 0.37 for Fig. 4a and 0.23 and 0.33 for Fig. 4b. The interaction between site, soil depth, and distance to tree base was not significant. In Amoutchou, a hardpan prevented to measure the taproot diameter below 100 cm. The different sites were analyzed together. The standard error of the difference between log-transformed data to compare sites at similar depths is 0.22 and to compare depths at similar sites is 0.13. Glidji Amoutchou SarakawaIn many areas of Zimbabwe, farmers store manure for up to three months for use on field crops especially maize and finger millet. There are several manure storage techniques used, the predominant being heaping (Nzuma, Murwira and Mpepereki, 1998). Storing in pits (anaerobic composting) is a recent innovation that some farmers have tested (Nzuma and Murwira, 2000). This study assessed the profitability of pit and heap stored manure on maize production over a three-year period.The study was based on trials at Nhapi, Musegedi and Manyani in the Murewa Communal area from 1997/98 to 1999/2000. Manure which had been stored in pits and in heaps was applied at a rate equivalent to 100 kgNha -1 in the first season only. No manure was applied to control plots. Ammonium nitrate fertiliser was applied at 100 kgha -1 as top dressing yearly to all crops. Maize yield was measured over the three years. Grain price for the three seasons was obtained from the Grain Marketing Board and details of variable inputs were collected from Zimbabwe Farmers Union and the Department of Agricultural and Technical Extension Services.Information on the labour involved in heap and pit storage was obtained from thirty households which owned cattle. This covered digging and heaping manure and transporting it to the field, the labour and cost of digging a pit, digging manure in the kraal, putting in a pit, covering it and taking manure out of a pit and carrying to the field. Gross margin analysis, Net Present Value (NPV) and Student T-distribution were used. The gross margin was the difference between gross income and total variable costs whilst NPV was calculated as the present worth of benefits less the present worth of costs (Gittinger, 1982).The costs and benefits were discounted to reflect future values at 70%, this social discount needs to be high because the satisfaction of immediate needs is more urgent for most rural folk than the assurance of longer term benefits and also rainfall is unpredictable (Markandya and Pearce, 1991). Labour costs were deflated using annual inflation rates of respective years from 1998 to 2000 which were 37.2, 58.5 and 55.7 respectively (Reserve Bank of Zimbabwe, 2001). The corrected costs are included in the gross margin budget of these storage techniques during the year of manure application. Costs are in Z$ and a US$1 is equivalent to Z$55 as at August 2000.Of the 30 households interviewed, two families used pits only, four families used heaps only and 24 practised both. The mean number of days and costs of manure storage techniques are in table 1. Heaping required 1.0 to 9.0 days and pitting 2.5 to 11.0 days with means of 3.89 and 4.93 respectively. However, 219 there was no significant difference between these means ( 03 . 1 = − x , t = 1.759 and p= 0.084). Deflated costs of storage were used in the T-test. The cost of heaping varied from $18 to $231 and pitting ranged from $15 to $658 with means $87 and $133 respectively. Again, there was no significant difference between these costs ( 00 . 46 = − x ,t = 1.628, p = 0.110). In the year of manure application, pit stored manure had the largest gross margin ($2 184) and it was the only viable system (Table 1). Heaping (-$330) and the control (-$1363) both had negative gross margins which mean that they are unviable in this period. The adjusted yield from pit-stored manure was 5290 kgha -1 while heaping had 2600 kgha -1 (Figure 1).In a laboratory analysis by Nzuma and Murwira (2000), pit stored manure had a higher N content (2.51% N) compared with 1.12% N for heaped manure at the time of manure application. This resulted in rapid nutrient release from pitted manure during the season hence higher yields. Therefore, manure quality affects profitability by dictating yield level. Heaping produces aerobically decomposed manure which has few nutrients available during the year of application. This would cause higher yield with pits in the first season.Total costs, benefit streams and net incremental benefits are in Table 2. The profits realised from use of heaped manure increased over the three years while those from pit manure fell. Despite this, Mugwira and Mukurumbira (1986) found that with cattle manure yields are often higher with the second crop compared with the first. Total profit and yield of pit stored manure were greater than heaping because poor quality manure produced by heaping has a more pronounced residual effect than pitted manure. Costs of pit storage were higher ($9223) than for heap storage ($8809). The farmers' profits are not necessarily affected by the residual effect of cattle manure. Because of the residual effect, profits were expected to be greater for heaped manure than for pit stored but discounting of future benefits and costs offset this.Pit storage of manure is more profitable for maize than heaping in the year of application and over three-years even though yearly profits and yields decreased. Heaping had a more pronounced residual effect in the second and third seasons. Food legumes remained to be important components of various farming systems of Eastern Africa, while the attempt to integrate fodder legumes and legume cover crops (LCCs) since 1930s became unsuccessful. Farmers remained reluctant to integrate fodder legumes and LCCs, despite recognising their benefits as soil fertility restorers and high value feeds, mainly due to community/farmer specific socioeconomic factors. Farmers' participatory research was conducted in Ethiopian Highlands to understand the processes of integration of legumes of different use into mixed subsistent farming systems. Areka had an altitude of 1990 masl, and rainfall amount of 1300mm, which is characterised by poor access to resources, intensive cropping, land shortage and soil degradation. Firstly participatory evaluation was conducted on the agronomic performance and adaptability of eight legumes during the main and small growing seasons of 2000 and 2001. The treatments were Vetch, Stylosanthus, Crotalaria, Mucuna, Canavalia, Tephrosia, Field pea and Common bean. Following the agronomic evaluation, the perception of farmers to legumes of different use, the socio-economic factors dictating choices and adoption, and potential niches for legume integration into the cropping systems were considered. Dry matter production among legumes was significant regardless of the length of growing period. For short term fallows, 3 months or less, Crotalaria gave significantly higher biomass yield (4.2 t ha -1 ) followed by Vetch and Mucuna (2 t ha -1 ), while for medium-term fallow, 6 months, Tephrosia was best performing species (13.5 t ha -1 ) followed by Crotalaria (8.5 t ha -1 ). The selection criterion of farmers was far beyond biomass production. Farmers identified firm root system, early soil cover, biomass yield, decomposition rate, soil moisture conservation, drought resistance and feed value as important criteria. There was significant difference in soil moisture conservation among LCCs, and decreased in order of Mucuna (22.8%), Vetch (20.8 %), Stylosanthus (20.2 %), bare soil (17.1 %), Crotalaria (14 %), Canavalia (14 %) and Tephrosia (11.9 %), respectively. The overall sum of farmers' criteria showed that Mucuna followed by Crotalaria could be the most fitting species, but farmers finally decided for Vetch, the low yielder, due to its fast growth and high feed value because of their priority to livestock feed than soil fertility. The final decision of farmers for integrating a non-food legume into their temporal & spatial niches of the system depended on land productivity, farm size, land ownership, access to market and need for livestock feed. The potential adopters of LCCs and forage legumes were less than 7%, while 91% of the farmers integrated the new cultivars of the food legumes. After characterising the farming systems of other benchmark sites, those indicators were used for development of decision guides to be used for integration of legumes into multiple cropping systems of East African Highlands.Food legumes remained to be important components of various farming systems of Eastern Africa as they are the sole protein sources for animals and humans. Besides restoring soil fertility, legumes are grown in rotation with cereals mainly because they accompany the stable cereals in the local dishes. On the other hand, the attempt to integrate fodder legumes and legume cover crops (LCCs) since 1930s became unsuccessful. Farmers remained reluctant to integrate fodder legumes and LCCs, despite recognising their benefits as soil fertility restorers and high value feeds, mainly due to community/farmer specific socio-economic factors. However, as farmers export both grain and stover from the field, the amount of legume residue left to the soil is too small to have a profound effect on restoration of soil fertility.Degradation of arable lands became the major constraint of production the Ethiopian Highlands, due mainly to nutrient loss resulting from soil erosion, lack of soil fertility restoring resources, and unbalanced nutrient mining (Amede et al., 2001). However, most farmers in the region have very low financial resources to combat nutrient depletion, and hence research should be directed to seek affordable and least risky, but profitable amendments necessary to keep nutrient balance neutral (Versteeg et al., 1998). In 1999 and 2000, researchers of the African Highlands Initiative (AHI) conducted farmers participatory research on maize varieties on a degraded arable land in Southern Ethiopia, Areka, by applying inorganic fertilisers. Although the soil is an Eutric Nitisol deficit in nitrogen phosphorus (Waigel, 1986), high level application of inorganic N and P did not improve maize yield. Lack of response to inorganic fertilisers because of low soil organic matter content was also reported elsewhere (Swift and Woomer, 1993). Organic inputs could increase the total amount of nutrients added, and also influence availability of nutrients (Palm et al., 1997). However, more than 50% of the organic resource available in the region is maize stalk, of which 80% is used as a fuel wood (Amede et al., 2001). The strong competition for crop residues between livestock feed, soil fertility and fuel wood in the area limits the use of organic ferilizers unless a suitable strategy that builds the organic resource capital is designed. Fallowing for restoration of soil fertility is no more practised in the region due to extreme land shortage.One strategy could be systematic integration of legume cover crops into the farming system. Organic inputs from legumes could increase crop yield through improved nutrient supply/availability and/or improved soil-water holding capacity. Moreover, legumes offer other benefits such as providing cover to reduce soil erosion, maintenance & improvement of soil physical properties, increasing soil organic matter, cation exchange capacity, microbial activity and reduction of soil temperature (Tarwali et al., 1987;Abayomi et al., 2001) and weed suppression (Versteeg et al., 1998). There are several studies in Africa that showed positive effects of Legume Cover Crops (LCCs) on subsequent crops (Abayomi et al., 2001;Fishler & Wortmann, 1999;Gachene et al., 1999;Wortmann et al., 1994). Studies in Uganda with Crotalaria (Wortmann, et al., 1994;Fishler and Wortmann, 1999), and in Benin with Mucuna (Versteeg et al., 1998) showed that maize grown following LCCs produced significantly higher yield than those without green manure. The positive effect was due to high N& P benefits and nutrient pumping ability of legumes from deeper horizons. However, the success rate in achieving effective adoption of LCCs and forage legumes in Sub-saharan Africa has been low (Thomas and Sumberg, 1995) since farmers prefer food legumes over forage or/legume cover crops in that the opportunity cost is so high to allocate part of the resources of food legumes to LCC. Therefore, there is a need to develop an effective guideline that targets different legume types in different niches of different agro-ecologies and socio-economic strata.The objective of this paper was, therefore a) to analyse the distribution of legumes in the perennial-based (Enset-based) systems, b) test the performance of legumes under short term and medium term periods, c) identify the potential causes of non-adoption of LCC, and d) develop preliminary decision guides that could be used to integrate LCC in small scale farms with various socio-economic settings.The research was conducted at the Gununo site (Areka), Southern Ethiopian Highlands. It is situated on 37 o 39' E and 6 o 51' N, at an altitude range between 1880 and 1960 m.a.s.l The topography of the area is characterised by undulating slopes divided by v-shaped valleys of seasonal and intermittent streams, surrounded by steep slopes.The mean annual rainfall and temperature is about 1350 mm and 19.5 o C, respectively, with relatively low variability, in terms of amount of precepitation, over the years. The rainfall is unimodal with extended growing periods from March to the end of October, with short dry spell in June. The highest rainfall is experienced during the months of July and August and caused soil loss of 27 to 48 t ha -1 ( SCRP, 1996). The dominant soils in the study area are Eutric Nitisols, very deep (>130 m), acidic in nature. These soils originated from kaolinitic minerals which are inherently low in nitrogen and phosphorus (Waigel, 1986). Soil fertility gradient decreases from homestead to the outfield due to management effects.The research site has relatively very high human population density with an average land holding of 0.5 ha household -1. Using LCCs for soil fertility purposes is not a common practise in the area. LCCs were introduced into the system in 2000 following a farmers field school (FFS) approach so as to allow farmers to learn and appreciate various legumes uncommon to the area. The farmers research group (FRG) was mainly composed of mainly men, despite the repeated temptation of researchers to include women. The legumes were planted in two planting dates. The on-farm experiments, used simultaneously for FFS and also for evaluation of biomass productivity and after effect of legumes on the following maize crop, were planted on April 25, 2000 and July 1, 2000 and harvested on October 6, 2000 and January 6, 2001, respectively, using recommended seed rates. The interest of the farmers was to evaluate the effect of planting dates and length of fallow period on biomass productivity of respected species, and to identify the best fitting legumes for a short-term fallow (three months) or medium term (six months) fallow. Longterm fallow became impractical due to land scarcity. Thirty interested farmers, who were organised under one farmers research group (FRG), have studied six different species namely, Stylosanthus (Stylosanthus guianensis), Crotalaria (Crotalaria ochroleuca), Mucuna (Mucuna pruriens), Tephrosia (Tephrosia vogelii), Vetch (Vicia dasycarpa) and Canavalia (Canavalia ensiformis). All LCC were exotic species to the system except Stylosanthus. We also included two food legumes, namely common bean (Phaseolus vulgaris) and Pea (Pisum sativum), in the study that were existing in the farming system. The FRG studied and monitored growth and biomass productivity in short and long seasons of 2000. The researchers were involved mainly in facilitation of continual visits and stimulation of discussions among farmers. Farmers and researchers were recording their own data independently. After intensive discussion, the FRG identified six major criteria to propose one or the other legume to be integrated into the system. Since farmers considered soil water conservation as one important criterion for selecting LCCs, soil water content was determined under the canopy of each species at top 25-cm depth gravimetrically. Sampling was done in relatively dry weeks of November 2000, five months after planting. We considered four samples per plot, weighed immediately after sampling, oven dried the samples with 120 o C for a week before taking dry weight. Legume ground cover was determined using the beaded string method, knotted at 10-cm interval and laid across the diagonals of each plot, 12 weeks after planting. A supplemantary replicated on-farm experiment (a plot size of 12 m 2 , three replications) was conductedto evaluate biomass production of LCCs under partially controlled replicated experiment to verify earlier obtained results. It was also meant to identify the most promising species for short term fallow, as farmers were reluctant to allocate land for LCCs beyond three months. The species were planted on October 12, 2001 and harvested on January 10, 2002. The legumes received phosphorus at a rate of 30 kg/ha P 2 O 5 at planting. After four months of vegetative growth, the green biomass of the legumes was weighed and incorporated directly to the soil. Maize (var A511) was planted about one month after incorporation on all plots. Three additional nitrogen treatments were included namely, 0 N, 30 N and 60 N per hectare to draw a nitrogen equivalent curve.In August 2002, after farmers monitored the introduced legumes, 26 farmers from four villages selected species of their choice LCC and tested them in their farms together with a food legume, Pea. During the growing seasons of 2000 and 2001, we monitored which farmer selected what, how did they manage the LCCs in comparison to the food legume and for what purpose the legumes were used. Biomass production of the various legumes under farmers' management was also recorded. Besides structured questionnaire and formal survey (Pretty et al., 1995), an informal repeated on-field discussion using transect walks were used to identify the socio-economic factors that dictated farmers to choose one or the other option and to prioritise the most important criteria of decision making using pair wise analysis matrix. More over, farmers invited non-participating neighbouring farmers for discussion; hence the decision made is expected to represent the community.The tested species were those most favoured by farmers for further integration namely Crotalaria (Crotalaria ochroleuca), Mucuna (Mucuna pruriens), Tephrosia (Tephrosia vogelii), Vetch (Vicia dasycarpa) and Canavalia (Canavalia ensiformis) replicated three times arranged in a randomised block design. The plot size was 12 m2, with one-meter gangway between treatments. The field was weed free through out the season by hand weeding. In all cases, phosphorus was applied at a rate of 13-Kg ha-1 to facilitate growth and productivity. Data on biomass production of the species was analysed by ANOVA using statistical packages (Jandel Scientific, 1998).Using the qualitative and quantitative data obtained from the site, and by considering the hierarchy of indicators identified by farmers, we developed draft decision guides on the integration of legumes into the farming systems of the Ethiopian Highlands.The major land use systems in the community include homestead farms, which are characterised by soils with high organic matter content due to continuos application of organic residue. These soils are dark brown to black in colour mainly due to high organic matter content. This part of the farm was used to grow the most important crops such as enset (Enset ventricosum), coffee, vegetables, planting materials for sweet potato and raise tree seedlings are grown. In the system only about 3% of the homestead are occupied by legumes intercropped under the enset/ coffee plants (data not presented). Farmers are not applying inorganic fertiliser in this part of the farm. The homestead field is followed by the main field, which is characterised by red soils. Red soils are considered by the farmers as less fertile due to limited application of organic inputs, hence require application of inorganic fertiliser to get a reasonable amount of yield. In this part of the farm, farmers grow maize in association with taro, beans and sweet potato. This is also where legumes are growing most. The outfield is the most depleted and commonly allocated for growing maize or potato using inorganic fertlizers. This plot does not receive any organic manure, legumes are rarely planted and the crop residue is even exported for different purposes. Farmers do not practice intercropping in this part of the land. Although legumes are major components of the system, the primary objective of the farmers is production of food grains as sources of protein followed by feed production as a secondary product, but not soil fertility. That is also partly the reason why the amount of land allocated for legumes decreases with distance from the homestead (decreasing soil fertility).The rainfall distribution was favorable and there was no extended dry spell within the growing season of 2000 and 2001. For the medium-term fallow, Tephrosia produced the highest dry matter biomass yield, 13.5 t ha -1 followed by Crotalaria, 9 t ha -1 . In the three months growing period, the herbaceous legumes varied in biomass productivity significantly. Crotalaria and vetch were fast growing and also early maturing than the others. On the other hand, tephrosia was growing relatively slow at the initial stage of growth, which is reflected in the biomass accumulation. Accordingly, the biomass yield of crotalaria was significantly higher than the other legumes, while the biomass of tephrosia was much lower than all the others (Fig. 1). A similar experimental result was also obtained in the previous seasons on onfarm trials. Most of the biomass accumulation in Tephrosia was observed four months after planting. For the shortterm fallow, Crotalaria was the best performing species followed by Mucuna and Vetch. On individual farmer's field, Crotalaria was the best performing species regardless of soil fertility. Similar results were reported from Uganda (Wortmann et al., 1994). On the other hand, vetch and mucuna were performing best in fertile corners of the farms. This did not agree with the findings of Versteeg et al., (1998), which indicated that mucuna performed better than other green manures (including crotalaria) to recover completely degraded soils. When those species were planted in the driest part of the season, crotalaria and mucuna performed best and produced up to 2.9 t ha -1 dry matter with in three months of time (data not presented). Besides dry matter yield, we measured soil water content under the canopies of LCCs. The data showed that, the highest soil water content was obtained from mucuna and stylosanthus, which could be due to the self-mulching (Table 2). The ground cover (%) was the highest for Mucuna (100 %), and the lowest for vetch (60%). A similar result was obtained for mucuna in western Nigeria (Abayomi et al., 2001). Higher soil water content under mucuna &, stylosanthus implies that these species could improve soil water availability through reduction of evaporative loss if grown in combination with food crops.The result showed that maize grown after legumes produced significantly higher grain yield than the check (maize grown with out nitrogen fertiliser) and gave a maize yield at least equivalent to 30 kg of N/ha regardless of the legume species (Fig 1). The yield obtained from the plots of vetch, canavalia and mucuna was almost similar, while the yield obtained from crotalaria and tephrosia plots was significantly lower than that of the other species. Although the biomass of crotalaria incorporated to the soil was much higher than the others, the effect was not evident on maize yield. This could be explained by the fact that crotalaria had very high lignin content than the others at the time of harvesting and incorporation, which possibly affected the processes of decomposition and nutrient release.By considering the type of produce the farmers grow in the neighbouring field of equal size, which was sweet potato, and calcultating the costs and benefits of the LCCs and neighboring field, we found out that the opportunity cost of growing LCCs was much higher than anticipated. The maize yield gain obtained after growing LCCs in a short season should be more than two folds for the farmer to consider growing LCCs as potentially profitable interventions. Farmers evaluated the performance of LCCs in the fields individually or in groups through repeated visits. The selection criteria of farmers were beyond biomass production (Table 1). After intensive discussion among them selves, the FRG agreed on seven types of biophysical criteria to be considered for selection of LCCs (Table 1). However, the criteria of choice had different weights for farmers of different socio-economic category. None of the farmers mentioned labour demand as an important criterion. They considered firm root system (based on the strength of the plant during uprooting), rate of decomposition (the strength of the stalk and or the leaf to be broken), moisture conservation (moistness of the soil under the canopy of each species), drought resistance (wilting or nonwilting trends of the leaf during warm days), feed value (livestock preference), biomass production (the combination of early aggressive growth and dry matter production) and early soil cover. For resource poor farmers (who commonly did not own animal or own few) food legumes were the best choices. For farmers who own sloppy lands with erosion problems mucuna and canavalia were considered to be the best: Mucuna for its mulching behaviour and canavalia for its firm root system that reduced the risk of rill erosion. Farmers with exhausted land selected crotalaria, as all the other legumes were not growing well in the degraded corners of their farms. On the other hand, farmers with livestock selected legumes with feed value and fast growth (Vetch and Stylosanths). In general, Vetch was the most favoured legume despite low dry matter production, as it produced a considerable amount of dry matter within a short period of time to be used for livestock feed. It was also easy to incorporate into the soil and found it to be easily decomposable. The over all sum of farmers' ranking, however, showed that mucuna followed by crotalaria are the best candidates for the current farming system of Areka. Since Mucuna is aggressive in competition when grown in combination with other crops (Versteeg et al., 1998) it could be used to increase soil fertility in well established Enset/Coffee fields, while Crotalaria and Canavaia could be used to ameliorate exhausted outfields. Canavalia is found to be best fitting as an intercrop under maize as it has deep root system and did not hang on the stocks of the companion crop (personal observation). The herbaceous LCCs are reported to be of high quality organic resources (Gachene, et al., 1999) to be used as organic fertilisers directly to improve the grain yield of subsequent crops (Caamal-Meldonado et al., 2001;Abayomi et al., 2001). After thorough monitoring about the productivity and growth behavior of LCCs in the experimental plots, 26 farmers have tested various LCCs in their own farm. They tried mainly Canavalia, Crotalaria, Mucuna, Stylosanthus and Vetch. We documented that farmers selected the most degraded corners of the farm for growing LCCs and the fertile corners of their land for growing Pea (Table 2). About 50% of the trial farmers allocated depleted lands (degraded and abandoned) for the LCC. Further discussion with farmers revealed that they took this type of decision partly due to fear of risk, and partly not to occupy land that could be used for growing food crops. From the total respondents, 86.6% of the farmers knew about the role of green manures as soil fertility restorers (Fig. 2). However only 63% of them tested LCCs and of those who tested the green manures only 21 % responded LCCs were effective in improving the fertility status of the soil. About 79% believed that LCCs may not feet into their system mainly because they did not emerge well, or showed poor performance under depleted soils or are competing with food legumes for resources (labour, water and land) (Fig. 2). This was manifested by the fact that almost all of the farmers planted LCCs on the degraded corners of their farm ( Results from informal interviews followed by structured questioner showed that there are 21 different factors that affect the integration of legumes of different purposes. When farmers were asked to prioritise the most important factors that affect adoption and integration of legumes, farmers mentioned a) farm size b) suitability of the species for intecropping with food legumes c) productivity of their land d) suitability for livestock feed e) marketability of the product f) toxicity of the pod to children and animals g) who manages the farm (self or share cropping) h) length of time needed to grow the species and I) risk associated with growing LCCs in terms of introduction of pests and diseases. Earlier works suggested that farm size and land ownership effect integration of LCCs into small holder farms (Wortmann & Kirungu, 1999). After comparing those factors in a pair wise analysis, four major indicators of different hierarchy were identified (data not presented).1) Degree of land productivity: Farmers in Gununo associated land productivity mainly with the fertility status of the soil and distance of the plot from the homestead. The homestead field is commonly fertile due to continual supply of organic resources. Farmers did not apply inorganic fertiliser in this part of the farm. They remained reluctant to allocate a portion of this land to grow LCCs for biomass transfer or otherwise, but they grow food legumes, mainly beans, as intercrops in the coffee and enset fields. The potential niche that farmers were willing to allocate for LCCs is the most out field.2) Farm size: Despite very high interest of farmers to get alternative sources to inorganic fertilisers the probability that farmers may allocate land for growing LCCs depended on the size of their land holdings. For Areka conditions, a farm size of 0.75 ha is considered as large. Farmers with very small land holdings did not grow legumes as sole crops, but integrate as intercrops or relay crops. Therefore, the potential niches for LCCs are partly occupied unless their farm is highly depleted.3) Ownership of the farm: Whether a legume (mainly LCCs) could be grown by farmers or not depended on the authority of the person to decide on the existing land resources, which is linked to land ownership. Those farmers who did not have enough farm inputs (seed, fertilizer, labour and/or oxen) are obliged to give their land for share cropping. In this type of arrangement, the probability of growing LCCs on that farm is minimal. Instead, farmers who contracted the land preferred to grow high yielding cereals (maize & wheat) or root crops (sweet potato). As share cropping is an exhaustive profit-making arrangement, the chance of growing LCCs in such type of contracts was almost nil. Without ownership or security of tenure, farmers are unlikely to invest in new soil fertility amendment technology (Thomas and Sumberg, 1995) 4) Livestock feed: In mixed farming systems of Ethiopia livestock is a very important enterprise.Farmers select crop species/ varieties not only based on grain yield but also straw yield. Similarly legumes with multiple use were more favoured by the community than those legumes that were appropriate solely for green manure purposes.Above mentioned socio-economic criteria of farmers together with the productivity data from the field were used to develop decision guides to help farmers in selecting legumes to be incorporated into their land use systems as presented in Fig. 3. As mentioned above, farmers considered the degree of land productivity as the most important factor (placed at the highest hierarchy) for possible integration of legumes. Farmers who own degraded arable lands were willing to integrate more LCCs while those who own productive lands of large size wanted to grow food legumes with additional feed values. However, all farmers decided to have food legumes in their system regardless of farm size or land productivity. Beans and Pea are already in the system and farmers already found niches to grow them as they are also parts of the local dish. From the LCCs, farmers favoured vetch as mentioned above. Those farmers who wanted soil improving LCCs selected croletaria, as they found it better performing even under extremely degraded farms. However, about 45% the farmers with degraded arable lands are not willing to integrate LCCs, either because they did not manage their own farm, and practice share cropping /contract or have limited options of household income.In general, given very high population pressure and associated severe land shortage, farmers in Areka may not allocate full season for LCC, but preferred fast growing LCCs for short term fallow. The probability of integrating LCCs into the system became even less when the land is relatively fertile. As the homestead fields are relatively fertile and used for intercropping/relay cropping purposes, growing LCC on that part of the land may not be the choice of farmers. On the other hand, farmers with large farm size and high degree of land degradation may go for selected LCCs. The potential niche available in the system would be the least fertile most-out field where intercropping is not practised. The most out field is commonly occupied by potato in rotation with maize with relatively less vegetative cover over the years .The length of the growing period together with the amount and distribution of the rainfall dictates whether the system may allow growing legumes intercroped with maize, intercroped with perennials, or relay cropped with maize or sweet potato. In regions, where the growing season is extended up to eight months, and where the outfield became depleted to sustain crop production, LCCs that could grow under poor soil fertility conditions in drought-prone months would be appreciated. Indeed, crotalaria performed very well under such conditions.We are presenting three guidelines for integration of legumes into the farming systems of multiple cropping, perennial-based systems. The decision trees were developed based on the following back ground information from the site. 1) Farmers preferred food legumes over non-food legumes regardless of soil fertility status of their farm 2) The above ground biomass of grain legumes (grain & stover) is exported to the homestead for feed and food while the below ground biomass of grain legumes is small to effect soil fertility. The probability of the manure to be returned to the same plot is less as farmers prefer to apply manure to the perennial crops (Enset & Coffee) growing in the home stead.3) The tested legumes may fix nitrogen to fulfil their partial demand (we have observed nodules in all although we did not quantify N-fixation), but in conditions where the biomass is exported, like vetch for feed, most of the nutrient stock would be exported. Therefore, we did not expect significant effect on soil fertility. 4) LCCs produced much higher biomass when planted as relay crops in the middle of the growing season than when planted at the end of the growing season as short-term fallows due to possible effects of end-of season drought.5) The homestead field is much more fertile than the outfield; hence those legumes sensitive to water and nutrients will do better in the homestead than in the outfield. Fig. 3 Guideline for integration food, feed legumes and legume cover crops in small-scale farms.The first guide (Fig 2) is intended to assist researchers to get feed back information about technologies that were accepted or rejected by the farmers or farmer research groups. This guide will assist researchers not only to identify the major reasons for the technology to be accepted or rejected, but also to prioritise the reasons of resistance by farmers not to adopt the technology. This type of feed back will help to modify/improve the technology through consultative research to make technologies compatible to the socio-economic conditions of the community.The second guide (Fig 3) integrated both biophysical and socioeconomic indicators. The most important criteria at the lowest level is the presence or absence of livestock in the household followed by who manages the farm, market access, the size of the land holding and the land quality. The factor that dictates the decision at the highest level was land productivity, which was governed mainly by soil fertility status. Growing food legumes was the priority of every farmer regardless of wealth (land size, land quality & number of livestock). Farmers with livestock integrated feed crops regardless of land size, land productivity and market access to products. However, the size and quality of land allocated for growing feed legumes depended on market access to livestock products (milk, butter and meat). Those farmers with good market access are expected to invest part of their income on external inputs, i.e. inorganic fertilisers. Hence farmers of this category did not allocate much land for growing LCCs, but applied inorganic fertilisers. In the homestead field, there was no land allocated for LCCs in the system, not only because farmers gave priority to food legumes, but it also became very expensive for farmers to allocate the fertile plot of the farm for growing LCCs. The most clear spatial niche for growing LCCs is the most out field, especially in poor farmers' field with exhausted land and limited market-driven farm products. Because the land of most poor house holds was on the verge of being out of production due to the iniquitous nature of land management practices through years long share cropping arrangements.Land resource degradation is one of the major threats to food security and natural resource base in Ethiopia. Hundreds of years of exploitve traditional land use, aggravated by high human and livestock population density have led to the extraction of the natural capital, which caused the farming of uncultivable sloppy lands and overexploitation of slowly renewable resources. The outcome is that half of the highlands are eroded, of which 15% are so seriously degraded that it will be difficult to reverse them to be agriculturally productive in the near future. In the mountainous highlands, there is a direct link between land-based resources and rural livelihoods. Decline in soil fertility as a result of land degradation decreases crop/livestock productivity and hence household income. Depleted soils commonly reduce payoffs to agricultural investments, as they rarely respond to external inputs, such as mineral fertilizers, and hence reduce the efficiency and return of fertilizer use. Degraded soils have also very poor water holding capacity partly because of low soil organic matter content that in turn reduce the fertilizer use efficiency. There have been various attempts to reduce land degradation in Ethiopia since the 1970s, through national campaigns on construction of terraces, project afforstation programmes and policy interventions. The objective of this paper is to review the various research/development experiences on integrated soil fertility management and synthesize the positive experiences augumented by the experiences of the African highlands initiative on integrated land management in Ethiopian Highlands. The paper will also suggest an outline that could be used by farmers, researchers and policy makers to reverse the alarming trend of land degradation in the mountainous highlands.This work has consulted the available literature on land degradation and soil fertility management in Ethiopian highlands. While TSBF-CIAT/AHI has been working closely with the Ethiopian Agricultural Research Organisation (EARO) and the Buro of Agriculture, and conducting participatory research in two benchmark sites of the Ethiopian highlands on INRM issues, it became apparent that land degradation is the most fundamental threat for the Ethiopian Agriculture. Based on the systems intensification work that we have been conducting in the two benchmark sites of African highlands initiative, Areka and Ginchi, augmented by secondary data on relevant themes, the following approach was suggested to address land degradation in the country.There are multiple factors that cause land degradation at short and long terms in the region. In Sub Saharan Africa, the major bio-physical agents of land degradation are water erosion, wind erosion and chemical degradation that affected soil loss by 47, 36 and 12%, respectively. Given the mountainous and sloppy landscapes, the major environmental factor that causes considerable soil and nutrient loss within a short period of time is water erosion followed by wind erosion. Most of the Wollo and Shewa highlands became erosion-prone due to high rainfall intensity accompanied by very steeply farmlands. Recent surveys showed that erosion effect is severe in high rainfall areas predominantly covered by nitisols and vertisols. In about 40% of the highlands, the erosion effect was so severe that active erosion was transformed to passive erosion, and hence there are rarely visible signs of sheet or rill erosion, but gullies and land slides. The hazards of erosion in the region was accelerated by socio-economic factors, namely absence of land ownership rights that discourage long term investments, population pressure, lack of alternative income generating options, and weak social capital that failed to protect communal grazing lands, up-slope forest covers and water resources.Although the degree of soil erosion is highly related to the interaction of Wischmeier factors, the type of land use and management may have played an important role in the Ethiopian highlands. The contribution of different management factors towards land degradation in Africa is estimated to be 49%, 24%, 14%, 13% and 2% for overgrazing, agricultural activities, deforestation, overexploitation and industrial activities (Vanlauwe et al, 2002). The livestock sector is a very important component of the system both as an economic buffer in times of crop failure and economic crisis and as a supportive enterprise for crop production. There is a considerable concern, however, that the number of animals per household in Ethiopian highlands is much higher than the carrying capacity of land resources. Overgrazing due to very high livestock population density in the Amhara region is expected to contribute most to land degradation. For instance, the total annual feed available in the highlands is estimated to be about 9.1 million tones of biomass while the demand is about 21 million tones, double that of the carrying capacity of the land (Betru, 2002). Another very important factor that aggravated land degradation in the Ethiopian highlands is deforestation. The forest cover went down from 40% at the beginning of this century to less than 3% at present, due to ever-growing demand for wood products and very low commitment in planting trees mainly because of the prevailing nationalization of private woodlots in the 1970s and 1980s. Besides, a very high consumption of wood for fuel and housing, wood products, mainly charcoal, became a major cash generating activities in the country in recent years. Deforestation and overgrazing accelerated land degradation in many ways. Firstly a land without vegetative cover is easily susceptible to erosion, both wind and water, and hence causes a considerable nutrient movement. Secondly, a large amount of litter that could have contributed for maintaining soil organic matter and nutrient status is considerably reduced. Thirdly deforestation in the highlands caused lack of fuel wood, and hence farmers use manure and crop residue as cooking fuel, which otherwise could have been used for soil fertility replenishment.Over-mining of land resources with out returning the basic nutrients to the soil is also an important factor that contributed most for soil fertility decline in the region. For instance, barley is the single dominant crop in the upper highlands of Wollo. The system has very low crop diversity with legume component of less than 3%. The system receives external inputs very rarely with a fertilizer rate of less than 5 kg/ha (Quinones et al., 1997), and the practice of applying this limited amount of mineral fertilizer is a recent practice. Data from the region on the amount of nutrients returned to the soil in comparison to the nutrients lost through removal of crop harvest showed that only 18, 60 and 7 % of nitrogen, phosphorus and potassium is returned to the soil, respectively (Sanchez et al., 1997). Hence there is an over mining of nutrients from the same rhizosphere for years and years.Another cause of land degradation is lack of early awareness about land degradation by farmers, which is partly associated with the rural poverty. McDonagh, et al., (2001) reported that when farmers were asked to describe their indicators of soil erosion they stated gully/rill formation, exposed underground rocks, land slides, wash away of crops, shallowing of soils and siltation of the soil. Similarly farmers indicators of soil fertility decline include stunted crops, yellowing of crops, weed infestation, and change of soil color to red or grey. These are soil traits that appear in a much later stage of soil degradation, after the soil organic matter and nutrients of the soil are removed. If farmers respond to soil erosion at this stage, the probability of reversing the fertility status to its earlier value would be difficult.Application of small amounts of mineral fertilizer alone, as it has been practiced on the 0.5 ha demonstration plots by FAO and the ministry of Agriculture for years, did not improve crop productivity much. The failure of this mono-technology approach calls for an integrated nutrient management that suits local biophysical, social and economic realities. Integrated nutrient management technologies can be nutrient saving, such as in controlling erosion and recycling of crop residues, manure and other biomass, or nutrient adding, such as in applying mineral fertilizers and importing feed stuffs for livestock (Smaling and Braun, 1996).The traditional field operation in the Ethiopian highlands, which could be characterized by multiple tillage, cereal-dominated cropping and very few perennial components in the system, is very erosive for soils and nutrients. Continual farming in the high lands with out considering conservation measures caused severe land degradation. FAO study in Zimbabwe showed that each hectare of wellmanaged maize growing land lost 10 tones of soil. Depleted soils commonly reduce payoffs to agricultural investments for various reasons. Degraded soils rarely respond to external inputs, such as mineral fertilizers, and hence reduce the efficiency and return of fertilizer use. Degraded soils have also very poor water holding capacity partly because of low soil organic matter content that in turn reduce the fertilizer use efficiency. Results from the dry regions of Niger, Sadore, showed that application of fertilizer increased the millet yield by 71% and also improved the water use efficiency by 70% (Bationo et al., 1993). Hence improved soil fertility enhances the water use efficiency of crops in drought prone areas. Low soil organic matter accompanied by low soil water content may also reduce the bio-chemical activity of the soil that may affect the above and below ground biodiversity of the system. Degraded soils have also low vegetative cover that may accelerate further soil loss and runoff.The effect of soil fertility decline goes beyond nutrient and water losses. There are conviencing results showing that the incidence of some pests and disease is strongly associated with decline in soil fertility. Results from the Amhara and Tigrai region showed that the effect of the notorious parasitic weed, striga, on maize and sorghum was severe in nutrient depleted soil (Esilaba, et al, 2001). It was possible to decrease the population & the incidence of striga significantly by improving the fertility status of the soil through application of organic fertilizers. Similarly the incidence of root rots in beans, stem maggots in beans, take all in barely and wheat is associated with decline in soil fertility (Marschner, 1995). The positive effect of application of organic and inorganic fertilizer on the resistance of the host crop is mainly through improving the vigorosity of the plant at the early phonological stages. Amede et al., (2001) outlined the need for a combination of measures to reverse the trend of soil fertility decline in the African highlands as presented in the following section.There are about 40 different types of indigenous soil and water conservation practices in different parts of the Ethiopian highlands, ranging from narrow ditches on slopping fields in Wollo highlands to the most advanced & integrated conservation measures in Konso, Southern Ethiopia. However, those indigenous practices are location specific and variable in their effectiveness, and call for closer understanding before any attempt is done for scaling-up. However, there is a consensus among actors that any attempt to protect land resources and improve productivity in the sloppy highlands should integrate systemcompatible soil conservation measures. Research conducted in Andit tid and Gununo showed that increasing the vegetation cover of the soil could decreases soil loss and runoff significantly (SCRP, 1996). In Andit tid, the amount of soil loss due to water erosion was 230 t/ha/year under hacked plots. However, it was possible to reduce the soil loss to 30 t/ha or less under crop covers or fallow grasslands (SCRP, 1996). When a cropland covered by crops or grasslands is compared to a frequently hacked farmland, run-off was reduced by about 90 and 100 % and soil loss by 68%, respectively. Hence soil nutrient loss and runoff could be minimized through increasing the frequency of crop cover, especially by those crops with mulching habits and higher leaf area indexs. Moreover, results from SCRP showed that perennial crops like enset and fruit trees or annuals with mulching and runner habits could reduce erosion effects significantly. Recent simulation modules in Northern Ethiopia showed that crop lands allocated for cereal crops like teff were very prone to erosion (Woldu, 2002), and the authors proposed that growing small seeded cereals, like teff, in sloppy farmlands should be discouraged.There has been an attempt to control soil erosion and rehabilitate degraded lands through construction of farmland terraces in the Ethiopian Highlands starting from the early 1970s. The program was facilitated through the food-for-work scheme of the World Food Program, as a response to the frequent droughts of the 70s and 80s in Ethiopia. The program attempted to construct terraces on about 4 millions of hectares of farm land. In early 1990s, the annual physical construction of farmland terraces reached over 220,000 ha (Lakew, et al, 2000). However, as the campaign was trying to address the problem with out the full participation of the rural community, except selling labor, the farmers considered the activity as an external imposition and hence failed to develop sense of ownership. The consequence being that farmers failed to maintain the terraces and, in some case, farmers have destroyed the terraces for getting another round of payment. When farmers were asked to list the reasons for rejecting soil and water conservation technologies they listed five major driving forces (Amede, 2002, unpublished) namely high labor cost, decreased farm size due to terraces, its inconvenience during farm operations especially for U-turn of oxen plough, and inefficiency of the terraces to stop erosion as they were only physical structures without any biological component and technical follow-ups. By considering those farmers criteria and by adopting participatory planning and implementation approaches farmers have adopted and disseminated soil conservation technologies in one the African Highlands Initiative benchmark sites, Areka (Amede et al, 2001). The major driving force for the adoption of the technology was its integration with high value crops (e.g. bananas, hops) and fast growing drought resistant feeds (e.g. Elephant grass, pigeon pea) grown on the soil bunds. The sustainable integration soil & water conservation technologies also depend heavily on the effectiveness of by-laws that limit free grazing and free movement of animals especially during the dry spells. This requires the empowerment of the local and regional policies so as to facilitate the integration of natural resource management technologies to practices of local communities. Moreover, effective landscape management, in terms of controlling soil erosion, is possible only when there is a community collective action. Unless the landscape is treated as a single unit and involves all potential stakeholders, any individual intervention could provoke social conflicts. For instance, construction of soil conservation bunds and deforestation of forests at the upper slope of the Lushoto highlands, Tanzania, decreased the amount of water flew to the valley bottoms, and affected the vegetable production and income of other farmers.Building the organic matter of the soil and the nutrient stock in short period of time requires a systems approach. These include the combination of judicious use of mineral fertilizers, improved integration of crops and livestock, improved organic residue management through composting and application of farmyard manure, deliberate crop rotations, short term fallowing, cereal-legume intercropping and integration of green manures. Because of the inconsistent use of mineral fertilizers and the very limited returns of crop residues to the soil, most of the internal N cycling in small holder systems results from mineralization of soil organic N. Such process may contribute most of the N for the annual crops until the labile soil organic fraction (N-capital) are depleted (Sanchez et al., 1997).Apart from the occasional application of small amounts of mineral fertilisers, all other organic resources form the principal means of increasing soil nutrient stocks and hence soil fertility restorers in small-scale farms. If these approaches are used in combination and appropriately, they could reverse the trend and consequently increase crop yields and, thereby alleviate food insecurity. However, the continued low yields are an indication of insufficient inputs and/or inappropriate use of these technologies. The majority of the small-scale farmers are still aggravating the soil/plant nutrient deficit through improper land management and over-mining of the nutrient pool. However, there is still an opportunity to replenish the soil nutrient pool using integrated approaches depending on the degree of soil degradation, the production system and the type of nutrient in deficit.One potential source of organic fertilizer is farmyard manure. There is a large number of livestock in the Amhara region that could produce a considerable amount of manure to be used for soil fertility replenishment. However, there is a strong competition for manure use between soil fertility and its use as a cooking fuel. Recent survey in the upper central highlands of Ethiopia showed that more than 80% of the manure is used as a source of fuel. Only farmers with access to fuel wood could apply manure in their home steads. Experiences from Zimbabwe showed that most manures had very low nutrient content, N fertlizer equivalency values of less than 30%, sometimes with high initial quality that did not explain the quality of the manure at times of use (Murwira et al., 2002). This could be explained by the fact that most manures were not composed of pure dung but rather a mixture of dung and crop residues from the stall. Besides the quality the quantity of manure produced on-farm is limited. Sandford (1989) indicated that to produce sufficient manure for sustainable production of 1-3 tonnes/ha of maize it requires 10-40 ha of dry season grazing land and 3 to 10 of wet season Range land, which is beyond the capacity of Ethiopian farmers. Moreover, the potential of manure to sustain soil fertility status and productivity of crops is affected by the number and composition of animals, size and quality of the feed resources and manure management. Wet season manure has a higher nutrient content than dry season manure, and pit manure has a better quality than pilled manure. Similarly, Powell (1986) indicated that dry season manure had N-content of 6 g/kg compared with 18.9 g/kg for early rainy season manure when the feed quality is high.Another potential organic source is crop residue. Returning crop residue to the soil, especially of legume origin, could replenish soil nutrients, like nitrogen. However, there is strong tradeoff for use of crop residue between soil fertility, animal feed and cooking fuel. In the upper Ethiopian highlands crop residues are used as a major source for dry season feed and supplementary for wet season feed. Hence little is remaining as a crop aftermath to the soil. Although legumes are known to add nitrogen & improve soil fertility, the frequency of legumes in the crop sequence in the upper highlands is less than 10%, which implies that the probability of growing legume on the same land is only once in ten years. The most reliable option to replenish soil fertility is, therefore, promoting integration of multipurpose legumes into the farming systems. Those legumes, especially those refereed as legume cover crops, could produce up to 10 ton/ha dry matter within four months, and are also fixing up to 120 kg N per season (Giller, 2002). Those high quality legumes adapted to the Ethiopian highlands include tephrosia, mucuna, crotalaria, canavalia, and vetch (Amede & Kirkby, 2002). However, despite a significant after effect of LCCs on the preceeding maize yield (up to 500% yield gain over the local management) farmers were reluctant to adopt the legume technology because of trade-off effects for food, feed and soil fertility purposes (Amede, unpublished data, 2002). In an attempt to understand factors affecting integration of soil improving legumes in to the farming systems of southern Ethiopia, Amede & Kirkby (2002) identified the most important socio-economic criteria of farmers namely, land productivity, farm size, land ownership, access to market and need for livestock feed. By considering the decision-making criteria of farmers on which legumes to integrate into their temporal & spatial niches of the system, it was possible to integrate the technology to about 10% of the partner farmers in southern Ethiopia.Organic resources may provide multiple benefits through improving the structure of the soil, soil water holding capacity, biological activity of the soil and extended nutrient release, but it could be unwise to expect the organics to fulfil the plant demand for all basic nutrients. Most organic fertilizers contain very small quantities of some nutrients (e.g. P and Zn) to cover the full demand of the crop, and hence mineral fertiliser should supplement it. Combined application of organic fertilizers with small amount of mineral fertilizers was found to be promising route to improve the efficiency of mineral fertilizers in small holder farms. For instance, Nziguheba et al., (2002) indicated that organic resources enhanced the availability of P by a variety of mechanisms, including blocking of P-sorption sites and prevention of P fixation by stimulation of the microbial P uptake. Long term trials conducted in Kenya on organic and mineral fertiliser interaction also showed that maize grain yield was consistently higher for 20 years in plots fertilised with mineral NP combined with farmyard manure than plots with sole mineral NP or farmyard manure (S.M Nandwa, KARI, unpublished data 1997). Although most farmers are convinced of using farm-based organic fertilisers, they are challenged by questions like which organic residue is good for soil fertility, how to identify the quality of organic resource, how much to apply, when to apply, and what should be the ratio of organics to mineral fertilisers. This calls for development of decision support guides to support farmers' decision on resource allocation and management. Scientists from Tropical Soils Biology and Fertility Institute of CIAT developed decision guide to identify the quality of organic fertilisers based on the polyphenol, lignin and nutrient content as potential indicators (Palm et al., 1997). As those parameters demand laboratory facilities and intensive knowledge, Giller (2000) simplified the guide by translating it to local knowledge as highly astrigent test (high polyphenol content), fibrous leaves and stems (high lignin content) and green leaf colour (high N content) to make the guides usable to farmers.In general, there is an increasing trend of mineral fertilizer use in the Ethiopian highlands over the past decades, and fertilizer imports into the country have increased from 47000 tonnes N & P in 1993 to 137 000 tones in 1996 (Quinones et al., 1997). It was mainly as a result of a strong campaign of Sasakawa-Global 2000 in collaboration with the Buro of Agriculture. However, there is a declining trend in fertilisers use in 2001/2002 due to increasing cost of fertilizers, lack of credit opportunities to resource poor farmers and low income return due to market problems.Sustainable rural development and natural resource management in the region demands an investment in and improvement of the natural capital, human capital and social capital. As the natural capital in the region had multiple problems that needs multiple solutions, there is a strong need for holistic approach to deliver options for clients of various socio-economic categories.Given the complexity of the problem of land degradation, and its link to social, economical and policy dimensions, it requires a comprehensive approach that combines local and scientific knowledge through community participation, capacity building of the local actors through farmers participatory research and enhanced farmer innovation. This approach requires the full involvement of stakeholder at different levels to facilitate and integrate social, biophysical and policy components towards an improved natural resource management and sustainable livelihoods (Stroud, 2001). Watershed management as a unit of planning and change imposes the need for increased attention to issues of resource conservation and collective action by the community. The issues of land degradation may include afforstation of hillsides, water rehabilitation and/or harvesting and soil stabilization, soil fertility amendment through organic and mineral fertilizers and increasing vegetation cover by systematic use of the existing land and water resources. This could be achieved by working closely with communities and policy implementers in identifying and implementing possible solutions to address land degradation and other common landscape problems, like grazing land improvement, gully stabilization and by monitoring and documenting the processes for wider dissemination and coverage. Some of the watershed conservation related solutions should be tried and implemented on specific test locations using farmers' own contribution and the INRM team's technical supervision. However, a wider application of these solutions to larger areas may require attracting additional funding investments from the district, donors or other NGOs in the area. The local village communities may also effect changes in the norms and rules governing the use of natural resources in their vicinity. Traditional rules and local by-laws (e.g. written and unwritten and called \"afarsata\" or awatcheyache) regarding the use and sharing of resources exist in most villages and these need to be identified and studied with a view to effect reform or renew their emphasis in the community. Integration of Agroforestry technologies in the farming systems of the Ethiopian highlands failed because of absence of national and/or local policies /by-laws that prohibit free grazing and movement of animals in the dry season. Experiences from the 1980s campaign of 'Green Campaign' in Ethiopia also showed that it is almost impossible to address the issue of land degradation without the full involvement and commitment of the local community. The local by-laws in resource arrangement and use should be facilitated and supported, as the rules and regulations at the local level could be implemented effectively through elders and respected members of the community with tolerance and respect. There may be a church and/or witchcraft dimensions to these, and there may be changes over time that might help to understand why people are doing what they are doing. In addition, the influence of national and regional policies on local resource management should be understood. These will form an important subject of community wide discussion and deliberation (Stroud, 2001). The current undertaking of soil and water conservation practices through voluntary participation campaign of the community in the northern Ethiopian Highlands is one positive step forward for initiating collective action.The West African Semi-Arid Tropics is the home of the world's poorest people, 90% of whom live in villages and depend for their livelihood on subsistence agriculture. In this zone, the length of crop growing season ranges from 75 to 150 days. Recurrent droughts, soils of poor native fertility, wind erosion, surface crusting and low water-holding capacity are the main abiotic constraints to crop production.In traditional agricultural systems, when crop yields declined to unacceptable levels, overcropped land was left to fallow until soil fertility was built up, and new land was opened for cultivation. Increasing population pressure is decreasing the availability of land and is leading to reduce duration of fallow relative to the duration of cropping. As a result, shifting cultivation is losing its effectiveness and soil fertility is rapidly declining in many areas. The present farming systems are unsustainable without external inputs of nutrients, will continue to be low in productivity and have long-term destructive potential to the environment. In such systems, plant nutrient balances are negative (Stoorvogel and Smaling, 1990).Among soil fertility factors, phosphorus deficiency is a major constraint to crop production and response to nitrogen is substantial only when both moisture and phosphorus are not limiting (Traoré, 1974). Although lack of water limits crop production in the drier zones in the Sahel, all available evidences indicates that inherent low fertility (mainly P) is a more serious problem (Breman and de Wit, 1983;van Keulen and Breman, 1990).For many years, research has been undertaken to assess the extent of soil phosphorus deficiency, to estimate phosphorus requirements of major crops, and to evaluate the agronomic potential of various phosphate fertilizers including phosphate rock (PR) from local deposits (Goldsworthy, 1967a and1967b;Pichot and Roche, 1972;Thibaut et al., 1980;Bationo et al., 1987;Bationo et al., 1990). In a survey of the fertility status of representatives sites, Manu et al. (1991) found that the total P in these soils ranged from 25 to 349 mg kg -1 with a mean of 109 mg kg -1 . Available P with Bray P1 was also generally low, ranging from 1 to 30 mg kg -1 with an average of 6 mg kg -1 . However, 77% of samples had available P values of less than 8 mg kg -1 which has been determined to be the critical P level required to obtain 90% of the maximum pearl millet yield in the sandy soil of Niger (Bationo et al., 1989a).The method of Fox and Kamprath (1970) was used to study the P-sorption characteristics of those soils and selected adsorption isotherms are presented in Figure 1. Sorption data were fitted to the Langmuir equation (Langmuir, 1918) and phosphorus adsorption maxima were calculated. From these representative sites, Manu et al., (1991) found that the values of maximum P sorbed ranged from 27 mg kg -1 to 253 mg kg -1 with a mean of 94 mg kg -1 . Soils of this region can be considered as having relatively low P sorption capacities compared to clay rich Utisols and Oxisols found in humid tropical regions (Sanchez and Uehera, 1980). As a consequence of the low P retention capacity of these soils, relatively small quantities of P fertilizers will be needed for optimum crop growth.Phosphorus use efficiency in this paper is calculated by dividing the difference in yield between P-treatment and control with the rate of P applied. In addition to the water soluble P sources such as single superphosphate (SSP) and triple superphosphate (TSP), phosphate rocks (PRs) indigenous to this region such as Tahoua PR (TPR) and Parc-W PR (PRW) from Niger, and Kodjari PR (KPR) from Burkina Faso were evaluated in field trials on the main soil types for crop production. In this region, use of water soluble imported P fertilizers is severely limited because of their high cost. The direct application of PR indigenous to the region may be an economical alternative to the use of more expensive imported P fertilizers. Some PRs may not be suitable for direct application because of their low chemical reactivity (Hammond et al., 1986). Partial acidulation of PR (PAPR) represents a technology that improves the agronomic effectiveness of an indigenous PR at a lower cost than would be required to manufacture the conventional, fully acidulated fertilizers from the same rock (Chien and Hammond, 1978;Hammond et al., 1986;Bationo et al., 1990).In this paper, after a brief review of the phosphorus use efficiency (PUE) of crops as effected by sources of P fertilizers, we will discuss the effect of rainfall, crop and soil management on Phosphorus use efficiency (PUE).Effect of P sources and rainfall on PUE Experiment on the evaluation of different sources of P fertilizers. From 1982 a benchmark field trial was initiated on the Sandy Sahelian soils of ICRISAT at Sadoré to evaluate the agronomic efficiency of different sources of P fertilizers. The sources of P fertilizers in those trials were Parc W phosphate rock (Parc W PR), Partially acidulated rock Parc W at 50 % (Parc W PAPR), Triple Superphosphate (TSP), and Single superphosphate (SSP). P was applied at 0, 4.8, 8.8, 13, 17.6 kg.ha -1 . Pearl millet cultivar CIVT was used as test crop. Experiment on PUE efficiency in different agro-ecological zones. In 1996 field trials were conducted at Sadoré, Gobery and Gaya to evaluate the agronomic effectiveness of Kodjari PR (KPR) and Tahoua PR (TPR) compared to single superphosphate (SSP).In a researcher managed trial at Karabedji, hill placement of small quantities of fertilizers were evaluated on water soluble and phosphate rock on pearl millet and cowpea. The two PR used were Tahoua phosphate rock (TPR) and Kodjari phosphate rock (KPR).Effect of mineral and organic fertilizers, ridging, and rotation of pearl millet and cowpea on PUE. In 1998 data were collected in an experiment to evaluate the effect of nitrogen application, crop residue, ridging and rotation of pearl millet with cowpea on PUE.From 1992 to 1995 an experiment was conducted to study the effect of crop rotation of pearl millet and cowpea on PUE at the ICRISAT Sahelian Center. Phosphorus was applied at 0, 6.5 and 13 kg.ha -1 as single superphosphate.For the benchmark experiment was conducted during the period of 1982-1987 SSP outperformed the other sources and its superiority to sulfur-free TSP indicates that with continuous cultivation, sulfur deficiency develops (Frisen, 1991). For both pearl millet grain and total dry matter yields, the relative agronomic effectiveness was almost similar for TSP as compared to PAPR with 50% acidulation (PAPR50) indicating that partial acidulation of PRW at 50% can significantly increase its effectiveness (Figure 2). SSP had the highest PUE values at all rates of P application. Increased rate resulted in a decrease in PUE. For pearl millet grain, application of 4.4 kg P/ha resulted in a PUE of 100 kg grain/kg P, but the PUE decrease to 45 kg grain/kg P at the rate of application of 13 kg P/ha. The difference between the P sources is better resolved at lower application rates of P fertilizers as compared to the higher application rates. The difference between PRW and SSP at 4.4 kg P/ha was 50 kg grain/kg P while at 17.5 kg P/ha, this difference was reduced to only 27 kg grain/kg P (Figure 2).For the trial for agronomic evaluation of P sources in different agro-ecological zones of Niger, the response of pearl millet to different sources of P fertilizers indicates that TPR agronomic effectiveness outperformed KPR (Figure 3 and Table 1). These results are in agreement with the fact that the molar PO 4 /CO 4 ratio is 23.0 for KPR and 4.88 for TPR, and TPR also has a higher solubility in NAC. Mokwunye (1995) found that the level of isomorphic substitution of carbonate for phosphate within the lattice of the apatite crystal influences the solubility of the apatite in the rock and therefore controls the amount of phosphorus that is released when PR is applied to soils. Chien (1977) found that the solubility of PR in neutral ammonium citrate (NAC) was directly related to the level of carbonate substitution. As a result of the higher value of Tilemsi PR in NAC, and the high substitution of carbonate for phosphate, Bationo et al. (1997) found that Tilemsi PR can result in net returns and value/cost ratios similar to recommended cotton or cereal complex imported fertilizers.The PUE at Gobery was 31 kg grain/kg P for TPR, but decreased to 9 kg grain/kg P with KPR application at 17KgP/ha. As soils in Gaya and Gobery are more acidic and receive more rain than the Sadoré site, the agronomic effectiveness is higher at those sites. The ability of the soil to provide the H + ions is essential to ensure the effectiveness of PR to crops (Chien, 1977;Khasawneh and Doll, 1978). Therefore, acidic soils with a high pH buffering capacity provide an ideal environment for PR dissolution.Results presented for upland rice by Bado et al., 1995 indicate that PUE of the unreactive Kodjari PR on an acidic (PH in H 2 0 = 5) soil is similar to the PUE of the water soluble TSP (Mahaman et al., 1998).The agronomic effectiveness of the leguminous cowpea is not better than the cereal pearl millet crop (Table 1). This is in contradiction to others reports where legumes have higher strategy to solubilize PR than cereal by rhizosphere acidulation (Aguilar and van Diest, 1981;Kirk and Nye, 1986;Hedley et al., 1982) and exudation of organic acids (Ohwaki and Hirata, 1992).The increase in soil pH resulting from flooding of rice fields is expected to depress the dissolution of PR. Enhanced performance of PR in flooded systems has been reported (Hammond et al., 1986). Kirk and Nye (1986) explain the enhance PR performance in flooded soils by arguing that rice roots will acidify surrounding soil and that dissolved organic matter may chelate Ca and P. In the irrigated system the PUE of PR often was higher than TSP (INRAN, 1988).Using data of PUE at 13 kg P ha -1 details from experiment presented in Figure 2 conducted over 10 years period, it was found that the rainfall received in September at grain filling and maturation stage was best correlated to PUE (Figure 4). From the results presented in Figure 4, it could be concluded that PUE of SSP was most affected by the amount of September rainfall due to its higher biomass production as compared PR or PAPR50. The predictions indicate that for SSP, a 40 mm rainfall in September will result in a PUE of 118 kg dry matter/kg P while for 100 mm rainfall the PUE will increase to 160 kg dry matter/kg P.In the West African Semi-Arid Tropics, both water and nutrients limits crop production, but from multi-location water-balance studies in Niger, it was shown that an important outcome of fertilizer use is an increase in water-use efficiency (Breman and de Wit, 1983;van Keulen and Breman, 1990). In longterm experiments, water-use efficiency (WUE) for grain yield increased dramatically from 5.4 kg mm -1 ha -1 without the use of fertilizers to 14.4 kg mm -1 ha -1 with the use of fertilizers. Increased root growth due to P application is associated with greater rooting depth and deeper extraction of moisture during dry spells (Payne and al., 1995). Early vigor and enhanced growth due to P application results in more complete ground cover early in the season, which reduces the proportion of water lost through water evaporation to some extent, thus facilitating effective and efficient use of rainfall.Although the application of fertilizers improves WUE, the efficiency of fertilizer depends on the amount of rainfall received by the crop. For nitrogen, Bationo et al. (1989b) developed a model relating grain yield of pearl millet to mid-season rainfall (45 days, from mid-July to end of August). This model predicts that response to N in dry years will be limited, with little benefit to the farmers from the investment in N fertilizers.Relationship between crop and soil management on phosphorus use efficiency Over a period of three years, nine pearl millet cultivars were evaluated to determine their PUE. For both grain and stover, there are very large differences among the nine cultivars for their response to the application of P fertilizer (Figure 5). PUE at 13 kg P ha -1 varied among the 9 cultivars from 25 kg grain/kg P for variety ICMV IS 85333 to 77 kg grain/kg P for Haini-Kirei cultivar the local is 3 weeks later to mature and has a very dense root system. Figure 6 shows PUE of different genotypes was significantly correlated with grain yield at 13 kg P ha -1 was, and explained 77% of total variation in this relationship. This significant relationship indicates that phosphorus use efficient cultivars can be first identified using their grain yield performance at 13 kg P/ha. The relationship between the PUE of the different genotypes with grain yield in the absence of P application was not significant and only 15% of the total variation could be explained. This observation shows that a cultivar with a high PUE coefficient will not necessarily perform better under low P conditions than the one with a low PUE coefficient. This also implies that genotypes selected for high grain yield under low-P situations will not necessarily be Puse efficient. There is ample evidence that indicates marked differences exist between species and genotypes for P uptake (Föhse et al. 1988;McLachlam, 1976;Caradus, 1980;Nielsen and Schjorring, 1983;Spencer et al. 1980).For the researcher managed on-farm trials conducted to study interaction between hill placement of small quantities of P fertilizers on the efficiency of water soluble (SSP, 3). Whereas PUE efficiency is 14 for pearl millet grain yield with KPR broadcast it increased to 31 kg grain/kg P when additional hill placement for 15-15-15 is applied (Table 2). Although hill placement alone of 4 kg P ha -1 gave high PUE values as compared to broadcast of 13 kg P ha -1 , this treatment will result in a net negative P balance. With the association of hill placement and low cost PR sources the net balance of P will be positive and soil mining will be avoided. For most of cases, 15-15-15 hill placement efficiency is higher than SSP hill placement. This is due in part to germination failures most likely due to deleterious pH and salt effects on the seedling. The highest effectiveness of NPK placement is also likely due to a stimulation of early root growth by the ammonium component (Marschner et al., 1986), and an enhanced availability of P in the immediate seedling environment. Over the past few years, on-station research at ICRISAT-Niger has focussed on the placement of small quantities of P fertilizers at planting stage in order to develop optimum farmer-affordable P application recommendation. Compared to control, millet grain yield increased between 60 to 70% when 5 kg P ha -1 was hill placed, and by 100% when 13 kg P ha -1 was broadcast. PUE on total dry matter and grain yield indicate that PUE at 3,5 and 7 kg P ha -1 hill application was higher as compared to broadcasting 13 kg P/ha. For example, in 1995, for total dry matter, the PUE for 13 kg P/ha was 159 kg TDM/kg P as compared to 402 kg TDM/kg P with the application of 3 kg P/ha hill placed. This is due in part to the placement of P where the soil is humid as compared to the surface broadcast where some fertilizers will remain in the dry zone of the soil (Muhelhig-Versen et al., 1997).In long-term soil management trials, application of nitrogen, crop residue and ridging and rotation of pearl millet with cowpea were evaluated to determine their effect on PUE. The results show that soil productivity of the sandy soils can be dramatically increased with the adoption of improved crop and soil management technologies. Whereas the absolute control recorded 33 kg ha -1 of grain, 1829 kg ha -1 was obtained when phosphorus, nitrogen and crop residue were applied to plots that were ridged and followed leguminous cowpea crop the previous season (Table 4). Results indicate that for grain yield, PUE will increase from 46 with only P application to 133 when P combined with nitrogen and crop residue applications and the crop is planted on ridge in a rotation system.In a study on the long-term effect of different cropping systems on PUE it was found that rotation of pearl millet with cowpea could significantly increase pearl millet and cowpea production (Figure 7). For pearl millet total dry matter, PUE increased from 149 kg ha -1 in the continuous cultivation to 252 kg ha -1 in rotation systems. For cowpea fodder, PUE increased from 40 kg ha -1 in the continuous cultivation to 65 kg ha -1 with rotation.In a long-term field trials to study the effect of crop residue application on PUE, PUE was 67 kg/kg P when only P fertilizers were applied, its value doubled when P fertilizers were combined with crop residue (Bationo et al., 1985).In the West African Semi-Arid Tropics, lack of volcanic rejuvenation has caused the region to undergo several cycles of weathering erosion, and leaving soil poor in nutrients.Both total and available P values are very low and P deficiency is a major constraint to crop production. With their sandy texture, these soils have low P retention capacity.The PUE is highly variable and depends on P sources, rainfall, soil and crop management. In the West African Semi-Arid Tropics there is little research on understanding the factors affecting P uptake such as the ability of plants to i) solubilize soil P through pH changes and the release of chelating agents and phosphates enzymes, ii) explore a large soil volume, and iii) absorb P from low soil solution P concentration.Genotypic improvement can come through increased capacity of plants to extract P from the soil or for decreased internal P requirement per unit dry matter produced. The opportunities for increased efficiency of P utilization through cultivar improvement include selection for treatments that favor strong plant demand such as late maturity, increased rootlet activity and increased P solubilization capacity.The available and total P values are very low in the region. With those extremely low values of total P, it can be questionable to select cultivar adapted to low P condition, as one cannot mine what is not there. Direct application of indigenous PR can be an economic alternative to the use of more expensive imported water-soluble P fertilizers.The effectiveness of mycorrhizae in utilizing soil P has been well documented (Silberbush and Barber, 1983;Lee andWani, 1991, Daft, 1991). An important future research opportunity is the selection of plant genotypes that are conducive to colonization by efficient Vesicular-Arbuscular Mycorrhizal (VAM) associations for better utilization of P from PR.Previous agronomic research has already identified a significant number of technologies to enhance PUE but future research needs to screen technologies under farmer's management in order to recommend with the highest economic returns. Agricultural sustainability implies that agriculture will remain the principal land use over long periods of time relative to human life-span and it is economically competitive and ecologically acceptable while the soil resource base maintains or even improves its fertility and health (Hamblin, 1991). One of the major challenges for the achievement of sustainable agriculture in the tropics, is the vulnerability of tropical soils to degradation when they are subjected to mechanization for crop production (Thomas et al., 1995;Thomas and Ayarza, 1999;Amézquita et al., 2000). It is widely believed that tropical savanna soils (mainly Oxisols) have excellent physical characteristics such as high infiltration rates, high permeability, good and stable soil structure and therefore can support mechanized agriculture (Sanchez and Salinas, 1981). However, recent work indicated that Colombian savanna soils (Oxisols of Altillanura), have serious physical, chemical and biological constraints for crop and pasture production (Amézquita et al., 1998a). Physically the fertile layer can be shallow with high bulk densities together with weak structure. Tillage (disc harrowing) practices currently used for seedbed preparation could result in surface sealing and low rainfall acceptance capacity (Amézquita et al., 2000). Chemically the soils have low pH values, high levels of exchangeable Al +3 , low P availability, low base (Ca, Mg and K) saturation and low amounts of organic matter. Also, biologically they show constraints typical of soils with low organic matter such as lower rates of mineralization (Thomas et al., 1995;Lopes et al., 1999).Physical, chemical and biological conditions of these soils need to be improved in order to increase their productivity. Usually this improvement can be achieved by land preparation and by application of lime and fertilizer. However, this effect lasts only for a short time and after 4 to 7 years, farmers abandon the degraded land as it is no longer productive and often migrate to other areas. To avoid the continued degradation of these soils and to achieve sustained production, we propose that the construction of an \"arable layer\", a top layer with improved soil properties, is required (Amézquita et al., 2000).It has been demonstrated that soil physical conditions are usually best under permanent grassland (or forest) and as soil is cultivated, these conditions deteriorate at a rate dependent of climate, soil texture and management (Lal, 1993;White, 1997). Amézquita et al. (1998a), have found significant negative effects of continued cropping on the physical properties of soils in the Llanos. The study by Preciado (1997) from the Casanare region of the Llanos showed that total porosity and macroporosity decrease markedly after 5-7 years of monocropping. Boonman (1997) mentioned similar trends for soils of African savannas.Ploughing and cultivating new land is usually accompanied by a decline in soil organic matter. When land is ploughed, disruption of peds exposes previously inaccessible organic matter to attack by microorganisms and populations of soil structure-stabilizing fungi and earthworms decrease markedly (White, 1997). Introduced pastures can markedly reverse these trends through improvements in soil aggregation (Drury et al., 1991;Gijsman and Thomas, 1995;Franzluebbers et al., 2000).The relatively weak structure of savanna soils of Colombia (Oxisols) and their susceptibility to sealing, compaction, and erosion when subjected to tillage can result in negative effects on sustainable productivity of crop-livestock systems (Amézquita, 1998). To overcome these physical constraints, tillage practices should be developed that are based on the concept of development of an \"arable layer\". The \"arable layer\" is a surface layer (0-15, 0-25, 0-30 cm depth), with improved soil physical, chemical and biological properties. This is essential for developing a soil that is capable to support sustainable agriculture (Amézquita et al., 2000).The \"arable layer\" concept proposed, is based on the combination of: (1) tillage practices to overcome soil physical constraints (high bulk density, surface sealing, low infiltration rates, poor root penetration, etc.). (2) use of chemical amendments (lime and fertilizers) to enhance soil fertility, and (3) use of soil and crop management practices to increase rooting, to promote biostructure, and to avoid repacking of soil after tillage, thus, improving the biological condition of the soil. This concept relies on the use of deep-rooted and acid soil adapted tropical pastures to improve and maintain soil physical conditions via vertical tillage (chisel).The purpose of this study was to evaluate the influence of deep-rooted tropical pastures in comparison with other land uses such as monocropping of upland rice and native savanna pastures on the build-up of an arable layer through improved soil properties.The experiments were carried out at Matazul farm (4º 9′ 4.9″ N, 72º 38′ 23″ W and 260 m.a.s.l.) located in the Eastern Plains (Llanos) near Puerto López, Colombia. The area has two distinct climatic seasons, a wet season from the beginning of March to December and a dry season from December to March and has an annual average temperature of 26.2 ºC. The area has mean annual rainfall of 2719 mm, potential evapotranspiration of 1623 mm and relative humidity of 81 % (data from the nearby Santa Rosa weather station, located at the Piedmont of the Llanos of Colombia). The soil has low fertility and the availability of P in the soil is low because of the soil's high P fixation capacity (Phiri et al., 2001).To evaluate the impact of deep-rooted pastures on soil physical characteristics, we used the following treatments from long-term experiments: a) Aggregate size distribution and aggregate stability aspects were studied in an experiment where disturbed and undisturbed introduced pasture systems were compared with rice monocropping on two sites of contrasting soil texture (Matazul: clay loam; Primavera: sandy loam). Native savanna (undisturbed) system was used as a control. Disturbed pasture received two harrow passes for every two years to reduce surface sealing and compaction. b) Infiltration rates were measured in an experiment aimed to improve top-soil conditions (cultural profile) using different intensities (1, 2 or 3) of chisel passes (vertical tillage) or different agropastoral treatments (pasture alone, pasture + legume and legumes alone) that were planted after 2 passes of chisel. c) Measurements on volume and chemical composition of gravitational water were studied in an experiment aimed to understand the processes of soil degradation due to either monocropping of rice or introduced pasture (Brachiaria dictyoneura cv. Llanero). Different number of harrow passes (2, 4, 8) were applied every year for a period of two years for each treatment. d) Root biomass and root volume of Brachiaria decumbens were determined in two contrasting textural soils: sandy-loam and clay-loam, under two pasture conditions: productive and degraded (less productive), to compare root growth under these two conditions.Aggregate size distribution and aggregate stability Ten volumetric soil samples were taken in cylinders (120 mm diameter by 25 mm high) and used for dry aggregate size distribution determinations from each of the following treatments: disturbed pasture, undisturbed pasture, monocrop and native savanna. Disturbed pastures means that two harrowing passes were made every 2 years to loosen the soil to improve pasture productivity. By the time of the evaluation, the experimental plots had 8 years of establishment. In each of the 10 samples taken from each treatment, a test for dry aggregate size distribution (Kemper and Rosenau, 1986;White, 1993;Amézquita et al., 1998b) was made using the total volume of soil collected in the cylinders. Sieves of the following openings were used: >6, 6-4, 4-2, 2-1, 1-0.5 mm, which were fitted to a shaker for 5 minutes.Aggregate stability was determined also using 10 samples (50 g of soil) for each treatment with a Yoder apparatus (Angers and Mehuys, 1993). A set of sieves with openings of: 2, 2-1, 1-0.5, 05-0.25, 0.25-0.125 and <0.125 mm was used. The amount of sand found in each sieve was discounted from the total weight.A double ring devise was used to determine infiltration rates (Bower, 1986). Five tests for each treatment were made. Internal cylinder was inserted into the soil to 5-7 cm soil depth. External cylinder was inserted to 3-5 cm. Water was poured first to the external cylinder to reach a height of about 3 cm within the cylinder and then to the internal cylinder to reach a height of 6 cm from the soil surface. The amount of water entering into the soil was measured at different time intervals during a testing period of two to three hours, until a quasi equilibrium of amount of water entering in function of time was reached.It is not common to collect and measure the amount and elemental composition of free water (drainage water) from the precipitation that moves down in a soil profile at different depths. In this study we determined the influence of pastures or monocropping of upland rice on the amount of gravitational water and its elemental composition at different soil depths. A pit of 1.8 m length × 0.7 m wide × 0.5 m depth m was dug in each treatment. Funnels filled with clean fine and very fine sand, were wetted to field capacity and then buried in the soil profile at different depths: 3, 5, 10, 15 and 30 cm to collect the gravitational water that passes through each depth, during part of the rainy season. Measurements of the amount of water and elemental composition, were made at different times. During the period of measurements, the pits were protected around and covered with a sheet of zinc to avoid any other water entering into the pit. This methodology assumes that there is a vertical piston like water movement. The accepted rain was assumed to move through the soil profile and reach the funnels that were buried at different depths. Wet sand present in the funnels favors pore continuity for the drainage process.Root sampling was carried out using trench profile method (Schuster, 1964). Three sampling points were randomly located within each treatment of degraded or productive pasture of Brachiaria decumbens. A trench of 60 cm wide, 50 cm deep and 60 cm long was dug to determine root penetration and root distribution. Root samples were excavated from the wall of each trench, totalling 3 samples from each treatment. The nail-boards were made of a 2 cm thick plywood board (50 cm wide and 40 cm long). Twelve cm long nails were inserted at 10 cm intervals (10 x 10 cm) through the back of the board and protruded into the frame 10 cm.Root samples were excavated by pressing the nail-boards into the trench wall and slicing the enclosed soil monolith from the trench wall with a steel blade. The samples were soaked in water for at least 2 h after which the soil was removed from the roots with a fine spray of water. The root samples were photographed. Root volume was determined with a measuring jar filled with water by registering the increase in volume. Root biomass (dry weight) was recorded after oven drying for 2 days at 65°C.Effect of different management systems.The aggregate size distribution under different management systems is shown in Table 1. At Matazul Farm, the percentage of aggregates >6 mm, 6-4 mm and 4-2 mm decreased in intervened systems compared with the native savanna, while those between 2-1 mm, 1-0.125 mm and <0.125 mm increased. This was noted particularly under monocropped rice. At La Primavera Farm, monocropping with rice resuted in a lower percentage of 4-2 mm and higher percentage of 2-1 mm and 1.0-0.125 mm aggregates. In contrast, the undisturbed pasture had a positive effect on soil aggregation, with the highest (non-significant) percentage of aggregates larger than 2 mm. The results on aggregate stability are presented in Table 2. Aggregate stability values at Matazul Farm were greater for native savanna than for intervened systems. The percentage of stable aggregates larger than 2 mm was significantly greater in relation to other treatments. At La Primavera Farm, undisturbed pasture and native savanna both had a higher percentage of aggregates larger than 2 mm diameter. Infiltration rates, determined under different management system treatments in an experiment aimed to create an arable layer, are shown in Table 3. In relation to native savanna the treatments that included introduced pastures showed higher and more stable rates. Particularly higher rates of infiltration were found under A. gayanus pasture. The amount of gravitational water draining at different soil depths as a function of soil management system is shown in Table 4. Little water was collected in the top layers of soil of savanna while greater amounts were collected at 15 cm soil depth. The treatment sown to upland rice with 8 harrow passes, did not allow the movement of free water through the soil. With 16 harrow passes more water was able to enter into the soil especially in the top two layers. Under introduced pastures, the amount of free water entering and moving through the soil profile was extremely high (480 cm 3 vs 0 cm 3 with 8 harrow passes and 490 cm 3 vs 100 cm 3 with 16 harrow passes) in comparison with upland rice.The chemical composition of the water collected at different soil depths under upland rice and pastures is shown in Table 5. Higher amounts of nutrients, especially at the first two depths were found under rice.Examination of soil monoliths collected through profile wall technique showed marked differences in root penetration and root distribution between a degraded pasture and a productive pasture of Brachiaria decumbens (Figure 1). Differences in root biomass and root volume at different soil depths, as influenced by soil texture (clay-loam and sandy-loam) are shown in Table 6. Clearly the productive pasture showed greater abundance and distribution of root systems than the degraded one.Good soil management should aim to create optimum physical conditions for plant growth (White, 1977). These include: a) adequate aeration for roots and microorganisms. b) adequate available water, c) easy root penetration, d) rapid and uniform seed germination, and e) resistance of the soil to slaking, surface sealing and accelerated erosion. Results from this study indicate that change in land use as deep-rooted tropical pasture can enhance soil quality by improving the size distribution of stable aggregates when compared with soils under continuous upland rice monocropping. The greater percentage of stable aggregates with introduced pastures compared with monocropping indicates that any kind of soil disturbance negatively affects aggregate stability, possibly through its influence on soil organic matter (Hamblin, 1985;Lal, 1993) or some of its components (Caron et al., 1992). Compared with native savanna, introduced pastures also showed higher and more stable rates of water infiltration, particularly with A. gayanus pasture. These results reconfirm the benefits of introduced pastures in improving soil quality (CIAT, 1998;Gijsman and Thomas, 1996).The improvement of the structural condition of soils by pastures, when they are used for grazing, normally change to less beneficial values of porosity, infiltrability, etc., as a consequence of trampling. However, strategies to maintain a good soil structural quality can be developed with proper grazing management.Little amount of gravitational water was collected in the top layers of soil of native savanna while greater amounts were collected at 15 cm soil depth suggesting the existence of preferential flow. This could be due to the wetting mechanisms dominant in the natural savannas. The treatment sown to upland rice with 8 harrow passes, did not allow the movement of free water through the soil, probably as a result of surface sealing that impeded the entrance of water. Under 16 harrow passes more water was able to enter into the soil especially in the first two depths, showing that there was a better rainfall acceptance under this treatment. The greater amounts of gravitational water entering and moving through the soil profile of introduced pasture in comparison with monocropping of upland rice indicates that introduced pastures are a very good alternative to improve and maintain the amount of macropores (pores that permit the free movement of water). This result confirms the beneficial effects of agropastoral system for improvement of these soils (Angers, 1992). Results on the chemical composition of the gravitational water collected indicate the beneficial effects of introduced pastures both on water and nutrient redistribution in the top-soil layers. However, it is important to note that pastures were sown a year before rice. ` Four aspects of the research deserve to be emphasized. First, the methodology used was appropriate as it was possible to collect drainage water and differentiate between treatments. Second, there was a very high variability in the way the water moved into the soil (preferential flow). Third, the amount of nutrients that moved from one depth to the other was a function of the total amount of water draining through soil profile. Fourth, the greater capacity of the pastures for facilitating a better movement and distribution of nutrients and water could be used for improving soil physical conditions.This study shows that change in land use as introduced pastures can enhance soil quality by improving the size distribution of stable aggregates, water infiltration rates and rainfall acceptance capacity when compared with soils under monocropping. We suggest that the intensive and sustainable use of these soils, is only possible if an \"arable\" or \"productive layer\" is produced and maintained i.e. a layer with little physical, chemical and biological constraints. One option to achieve this arable layer is the use of introduced pastures with deep rooting abilities that can result in increased soil organic matter and associated improvements in soil physical and chemical properties. One land management option that can achieve these improvements is agropastoralism whereby pastures and crops are grown in short-term rotations.The neotropical savannas occupy 243 million hectares in South America and are one of the most rapidly expanding agricultural frontiers in the world (Thomas and Ayarza, 1999). Oxisols predominate in the hyperisothermic savannas and cover an area of 17 million hectares in Colombia alone. Intensification of agricultural production in this ecosystem requires acid soil (aluminum) tolerant crop germplasm, soil fertility improvement and management of highly vulnerable physical properties (Amézquita, 1998;Guimaraes et al., 1999). Monocropping systems with high levels of inputs and excessive cultivation may be unsustainable since they may cause deterioration of soil physical properties as well as escalation of pest and disease problems.Improved legume-based pastures are considered least harmful to the soil resource base but require investments in inputs for establishment that are unattractive or beyond the means of graziers. Establishment of pastures in association with rice (to defray the cost of inputs) is a potential alternative that has seen significant adoption by farmers in frontier areas of the Colombian Llanos (Sanz et al., 1999). Alternative systems incorporating components that attenuate or reverse the deleterious effects of monocultures are required, and biophysical measures of sustainability need to be developed as 'predictors' of system 'health' to sustain agricultural production at high levels while minimizing soil degradation.Grain legumes, green manures, intercrops and leys are possible system components that could increase the stability of systems involving annual crops (Karlen et al., 1994). To test the effects of these components on system sustainability and to identify indicators of soil quality, a long-term field study was implemented in 1993 on a Colombian Oxisol under native savanna grassland using a selection of alternatives based on these components (Friesen et al., 1997). The study has extended through almost two cycles of the principal rotation, i.e., the agropastoral system, recognizing that the degrading or beneficial effects of various agricultural practices are often subtle and only manifest themselves over long periods. This paper presents results from the initial 5-year phase of the experiment, focusing on systems based on upland rice with emphasis on systems' effects on: (a) productivity; (b) soil fertility indicators; (c) soil physical attributes; (d) associated soil organic matter quality; and (d) soil biological health.The experiment was established on a well-drained silt clay loam (Tropeptic Haplustox, isohyperthermic) under native savanna grassland at Carimagua (4°37'N, 71°19'W, 175 m altitude) in the Eastern Plains of Colombia. The mean annual rainfall is 2240 mm with a mean temperature of 27°C. The experiment is laid out in a split-plot design with four replications in which alternative systems (in subplots, size 0.36 ha) based on upland rice or maize (main plots) are compared (Friesen et al., 1997). Only rice-based systems are reported here. They include rice monoculture, rice rotated with cowpeas (for grain), cowpea green manure (GM) or \"improved\" grass-legume pasture leys. Cowpea or GM rotations occurred within each year, i.e., rice was sown in the first season (semester) and the legumes in the second season annually. Pastures were sown simultaneously under rice in 1993 and again in 1998, and grazed in the intervening 4 years. Native savanna plots were maintained for baseline comparisons. Cropped systems were limed with 500 kg ha -1 of dolomite prior to establishment and maintained thereafter with annual applications of 200 kg ha -1 . Each rice crop received 80 kg-N ha -1 (split: 20+30+30), 60 kg-P ha -1 and 100 kg-K ha -1 . Legumes (cowpeas or GM) received 20 kg-N ha -1 , 40 kg-P ha -1 and 60 kg-K ha -1 . Pastures were fertilized biennially with 20 kg-P ha -1 . Plot sizes of 200 m × 18 m (3600 m 2 ) were used to allow for grazing by cattle and the use of conventional machinery which impact directly on soil physical properties especially. A description of treatments is provided in Table 1.Soils were sampled before planting rice each year from different systems including native savanna. The samples were air-dried, and visible plant roots were removed before they were gently crushed to pass a 2-mm sieve. The following chemical analyses were carried out: pH (1:1 soil:H 2 O ratio), exchangeable Al and Ca extracted in 1M KCl, and available P by the Bray-2 method. Soil pore-size distribution was determined from the moisture characteristic curves using undisturbed soil cores (50 mm × 25 mm) taken from the 0-10, 10-20 and 20-40 cm soil layers of each replicate (Phiri et al., 2001). Saturated soil cores were weighed and then subjected to different tensions (5, 10, 100, 300 and 1500 kPa). Pore-size distribution was calculated using the Kelvin equation. Pores were divided into macropores (>50 μm; drained at a tension of ≤6 kPa), mesopores (50-0.2 μm; water retained at >6 kPa but <1500 kPa) and micropores (<0.2 μm; water retained at >1500 kPa). Soil samples were taken for the 0-10 cm and 10-20 cm layers of each treatment in February 1998 in order to characterize the impact of production system on soil organic matter quantity and quality. Total soil C and N were determined by combustion on a Leco CHN analyzer and C:N ratio calculated. Size-density fractionation of soil organic matter (SOM) was done using the Ludox Method to separate three sizedensity fractions: LL (>150 μm, <1.13 g cm -3 ), LM (>150 μm, 1.13-1.37 g cm -3 ) and LH (>150 μm, >1.37 g cm -3 ) identified as most promising by Barrios et al. (1996).In June 1994 and June 1996 (rainy season), the earthworm community, comprising eight species native to the savanna ecology, were sampled by taking 25×25×30 cm soil monoliths at 50 to 120 points on a regular grid in each plot of each system. Samples were taken quickly, sorted and earthworm species identified and counted at each point. Earthworm biomass was estimated using available data of mean weights of each species at the period of sampling (Decaëns and Jiménez, 2002).Rice grain yields were measured each year in at least four 5m × 5m quadrats located randomly in each plot prior to harvesting the rice crop with a combine harvester. Grain, straw and weeds were separated, weighed and subsampled for moisture content and chemical analysis.Soil chemical characteristics under the different systems is shown in Figure 1 where systems are arranged from left to right in order of increasing intensity of input use and cultivation. Temporal changes in soil pH and exchangeable Al were very similar in all systems, with the exception of the inexplicably high Al values in the surface soil under pasture in the later years. The temporal fluctuations in soil pH and exchangeable Al observed in all soil layers can probably be attributed to variability associated with factors such as burning and temporary anaerobic conditions due to high rainfall which directly impact on soil pH and, consequently, soluble Al. Soil fertility indicators for P and Ca generally reflected increasing system intensity. In the absence of inputs to the native savanna system, no significant changes in available P or exchangeable Ca were observed during the five years of experimentation. P availability remained at low levels at all depths (0-10, 10-20 and 20-40 cm), and exchangeable Ca was higher in the surface soil (0-10 cm) than in subsoil layers. Under the rice-agropastoral system, available P and exchangeable Ca levels increased modestly with time in response to the initial applications of lime and P to the pioneer rice crop and the small biennial maintenance applications to the pasture thereafter. The resultant levels of available P (about 10 mg kg -1 ) are considered adequate for acid soil adapted forage germplasm.Under rice-monocrop system, P availability increased during the first three years in response to P fertilization but failed to reflect P additions in the latter two years, especially in the surface soil layer (0-10 cm). This could be due to P removal by weeds which became increasingly prevalent as the experiment progressed, and to P fixation by soil incorporated from subsoil layers through excessive ploughing (Friesen et al., 1997). The increase in available P in the 10-20 cm layer in 1998 supports this interpretation. Exchangeable Ca increased in all soil layers in response to annual lime applications. In the surface soil, the largest increase occurred in the first year and remained unvarying thereafter. Instead, annual Ca inputs were reflected in the 10-20 and 20-40 cm layers which progressively increased during the 5-year period, presumably due to leaching from the surface soil.Changes in soil pH and exchangeable Al were not well correlated with exchangeable Ca, contrary to expectations, probably due to the very low lime rates applied and Ca leaching as a neutral cation with nitrate or chloride which would not affect pH. Changes in exchangeable Ca under the rice-cowpea rotation were very similar to those under rice monoculture although movement of Ca into the subsoil was slightly less, perhaps due to scavenging by deep cowpea roots and cycling of Ca back to the surface through cowpea residues. In contrast, levels of available P in the 0-10 cm layer increased much more sharply over time and were accompanied by increased levels of available P in the subsurface 10-20 cm layer. These increases in available P reflect the additional applications of P fertilizer to cowpea component of the rotation while subsoil increases were probably the result of the increased frequency of cultivation required for the cowpea crop.The rice-GM system was the most intensely cultivated. Although inputs of lime and P fertilizer were the same as for the rice-cowpea system, there were some notable differences in available P and exchangeable Ca dynamics between the two. Exchangeable Ca in the 0-10 cm layer did not rise to the levels observed in either the rice monoculture or the rice-cowpea system, although changes in the subsoil layers were very similar. This can be explained by an increased rate of leaching of soluble Ca through the soil profile with the much higher nitrate concentrations generated by mineralization of ammonia produced by decomposing GM residues (Friesen et al., 1998). Available P followed a similar temporal trend to that observed in the rice-cowpea system in the first three years. However, in the latter two years, the increased intensity of tillage apparently caused some incorporation of P into the subsoil layer, resulting in a reduced level of available P in the surface soil and an increased level in the subsoil.The impact of the different crop rotation and ley pasture systems on some soil physical characteristics 5-years after establishment is shown in Table 2. In general, the saturated hydraulic conductivity of this Oxisol under native savanna is low in the surface soil and even lower in the subsoil layers. A hydraulic conductivity of 10 cm h -1 would be considered critical for the prevailing climatic conditions at Carimagua (2700 mm year -1 rainfall with high intensity 100-120 mm h -1 of rain storms). Most of the observed values were below this critical value. These results indicate that this soil has limited ability for downward movement of water, resulting in temporary waterlogging during intense storms. Infiltration of water through the soil profile is more critical with depth. Thus, any soil management strategy must include improvement of soil hydraulic conductivity. The various rice-based systems had no significant impact on hydraulic conductivity of the surface soil layer after 5 years of tillage at increasing levels of intensity. Measured two years later, chisel ploughing to 30 cm in the annual rotations and monoculture systems caused increased hydraulic conductivity in the 10-20 cm layer but not the 20-40 cm layer. Rooting of cowpeas in the subsoil apparently aided in maintaining the effects of chiseling more than rice alone. Statistically significant differences were found in bulk density among systems at different depths but the values found for 0-10 and 10-20 cm soil layers could be considered non-limiting for root growth and distribution. Below 20 cm soil depth where tillage implements (disc harrows) used for land preparation are not expected to have any direct impact, bulk density values were generally higher than those found in the ploughed layers. However, they were not substantially different than native savanna at that depth, indicating that land preparation was not causing added compaction in subsoil layers.Although some statistically significant differences in macroporosity were found among the different systems, values in the 0-10 and 10-20 cm soil layers are considered non-limiting for root growth and distribution. Below this depth, some values lower than the critical level (10%) were observed. Monocropping of rice resulted in marked decrease of macroporosity for 20-40 cm soil depth when compared with rotation systems.Trends among systems in total soil organic C and SOM fractions (i.e., LL-C) were generally the same (Table 3). However, SOM fractions were more sensitive to the effects of production system than conventional measures of total soil C. LL-C content revealed greater differences among treatments at 0-10 cm soil depth and also found significant effects at 10-20 cm depth. This agrees with results of Barrios et al. (1996Barrios et al. ( , 1997) ) where the LL-C fraction was identified as a sensitive indicator of SOM changes due to soil and crop management not detected by total soil C.Surface soil (0-10 cm) LL-C was usually higher than that of the sub-soil. Both total C and LL-C were highest in the agropastoral system and became progressively lower in the annual rice-based systems, in step with increasing intensity of cultivation in the order:Rice monocrop (1 cultivation yr -1 ) > rice-cowpea (2 yr -1 ) > rice-GM (3 yr -1 )Despite the large quantities of crop and GM residues incorporated into these systems, only the agropastoral system succeeded in building total SOM content; all other systems experienced declining total C values. Only the rice-GM system showed an increase in LL-C at 10-20 cm depth. Soil C:N ratio was significantly reduced in the surface soil of the rice-GM system, corresponding to the inputs of high quality organic residues. On the other hand, C:N ratio increased significantly in the agropastoral system, probably due to the high litter production of the grass component with its high C:N ratio. Lower soil C:N ratios in the rice-GM system are indicative of higher potential soil N availability, which can be equated with improved plant nutrition or alternatively greater potential for N loss from the system. High rates of legume residue decomposition in this experiment were reported previously (Friesen et al, 1998) and explain the failure to generate an increased SOM content in this system despite the high organic matter inputs.Intensification and land use system affected earthworm communities in different ways. One year after breaking native savanna (1993), a drastic reduction in earthworm density and biomass was observed in the established rice monocrop and agropastoral systems. Two years later (1996), earthworm density and biomass decreased sharply along a gradient in which highly intensified annual crop systems had deep detrimental impacts that were more accentuated in the rotations (i.e. systems that were tilled 2 or 3 time per year) -down to 3 individuals m -2 and 0.1 g m -2 in the rice-GM rotation from 50 individuals m -2 and 3.2 g m -2 in the native savanna (Decaëns and Jiménez, 2002).Earthworm species responded differently to intensification. Only one species, the small endogeic Ocnerodrilidae sp., seemed to be enhanced by the conversion of the savanna into annual crops which usually led to a drastic reduction of the number of species. Three species, Andiodrilus n. sp., Aymara n. sp. and Glossodrilus n. sp. often disappeared from the soil of these systems (Decaëns and Jiménez, 2002), although those species with a high surface mobility were able to colonize the agroecosystems again. Other species showed a high population growth potential that allowed them to recover to their population density before the perturbation. Sensitive species disappeared after pasture establishment but richness was recovered 3 years later.Average rice grain yields fluctuated from year to year in response to differences in moisture availability and, more importantly, increased competition from weeds (data not shown). The latter also resulted in increased variability and an inability to detect significant differences among systems in later years (Table 4). Rice-legume (cowpea or GM) rotations tended to produce greater yields throughout the 4year period following establishment in 1993; however, these were only statistically significant in 1994.With the exception of 1995, average rice grain yields did not show any apparent decline with time in any of the three annual production systems. ------------------------(Mg grain ha -1 ) - ----------------------- This 5-year field study examined the effects of contrasting rice-based production systems on rice productivity and indicators of soil chemical/fertility, physical and biological health. Increased intensity of fertilizer inputs associated with increased system intensity generally resulted in commensurate increases in soil fertility under those systems. A previous report (Friesen et al, 1998) showed increasing levels of inorganic N in soil profiles to 1-m depth under rice monoculture < rice-cowpea < rice-GM, with significant and substantial leaching due primarily to legume residues in the latter two systems. The longterm consequences and externalities of improved N fertility in such systems cannot be discounted.Soil physical characteristics were generally improved with increasing system intensity, probably due to the degraded nature of the soils under native savanna. Cultivation generally helped to create an 'arable layer' (Phiri et al, 2001) by incorporating immobile nutrients such as P to depth in this infertile Oxisol. However, these beneficial effects can only be considered short-term. Cultivation also resulted in declining levels of SOM, particularly in the LL-C fraction, which may have consequences on soil structure in the longer term.Soil macrofauna were the most adversely affected by production systems. Cultivation caused drastic reductions in earthworm populations and biomass, more severely so with increasing intensity and frequency. Since soil macrofauna have direct beneficial effects on many soil characteristics that affect its long term productivity (such as nutrient cycling, soil structure, soil water dynamics, bulk density and root penetrability), managing systems in ways that minimize the impact on macrofaunal populations will be an essential consideration in the sustainable use of this agroecosystem. Within the context of the savannas, Jiménez et al. ( 2001) proposed a hypothetical conservative agricultural production system to preserve benefits of soil fauna which integrated: (i) native vegetation plots possibly used as extensive pastures and as a reserve of biodiversity; (ii) permanent pastures for livestock systems that allow the establishment of important native earthworm biomass; (c) agro-pastoral systems with annual crops managed in rotation with temporary pastures and located contiguously to permanent pastures to maximize migration of populations. Integration of more intense production systems which build the 'arable layer' but thereafter revert to more conservative tillage practices may be viable alternatives whose sustainability should be examined at the landscape scale.In the humid tropics, a substantial proportion (36%) of agricultural land is on steep or very steep slopes (Wood et al., 2000). In mountainous regions of developing countries, these lands often play a central role in rural food security and increasingly supply urban and/or export food and forest product markets. Andean hillsides contribute to food production through agricultural systems but these systems are characterized by low productivity and limited use of nutrient inputs. They harbor a large proportion of the rural poor and are an important source of water for the urban population and agricultural and industrial activities downstream (CIAT, 1996a). Densely populated hillsides in the humid and sub-humid tropics are considered to be areas where diversification of cropping systems to include trees and shrubs could improve soil fertility, increase production of fuel-wood, and result in better watershed management (Young, 1997).Traditional agricultural systems in Colombia's tropical hillsides are based on shifting cultivation that involves slashing and burning of the native vegetation, followed by continuous cultivation and abandonment after 3-5 years because of low crop yields (Knapp et al., 1996). Leaving degraded soils to \"rest\" or \"fallow\" is a traditional management practice throughout the tropics for restoration of soil fertility lost during cropping (Sánchez, 1995). Successful restoration of soil fertility normally requires a long fallow period for sufficient regeneration of the native vegetation and establishment of tree species (Young, 1997). Increased pressure on land as a result of population growth has limited the possibility for long fallow periods. When purchasing power is low, one alternative to traditional fallows is to improve fallows with plants that replenish soil nutrient stocks faster than plants in natural succession (Barrios et al., 1997). Planted fallows are an appropriate technological entry point because of their low risk for the farmer, relatively low cost, and potential to generate additional products that bring immediate benefit while improving soil fertility (i.e. fuel-wood).Slash and mulch agroforestry systems include alley cropping systems where pruned biomass from tree rows is applied in the alleys between the rows before planting (Kang et al., 1990). Alternatively, biomass transfer systems include the harvesting and transporting of biomass from one farm location (e.g., live fences) to another as a source of nutrients for the crop (Jama et al., 2000). Fallow enrichment of traditional slash/mulch systems of 'frijol tapado' in Costa Rica have also shown the importance of the inclusion of trees as a source of biomass and nutrients during soil fertility recovery (Kettler, 1997). In the Honduran 'quezungual' system trees are left in cropped fields and pruned periodically to keep competition low while providing plant residues for soil cover and as a source of nutrients (Hellin et al., 1999).The volcanic-ash soils in Colombian hillsides generally contain high amounts of soil organic matter (SOM) but nutrient cycling through SOM in these soils is limited because most of it is chemically protected, which limit the rate of its decomposition (Phiri et al., 2001). The slash/mulch fallow system described in this work has the spatial design features of an agroforestry planted fallow system but involves prunings with the resulting biomass applied to the same fallow plot. This system is expected to accelerate nutrient recycling through increased biological activity in soils with high inherent nutrient reserves but low nutrient availability. In this paper we explore the agronomic features of this system as well as its impact on soil fertility recovery as measured by some soil chemical, physical and biological parameters before a cropping phase of maize.The study was conducted on two farms in Pescador, located in the Andean hillsides of the Cauca Department, southwestern Colombia (2º48' N, 76º33' W) at 1505 m above sea level. The area has a mean temperature of 19.3°C and a mean annual rainfall of 1900 mm (bimodal). The experiment started in November 1997 and the fallow phase concluded after 27 months (FebruaryMarch 2000).Soils in the area are derived from volcanic-ash deposition and are classified as Oxic Dystropepts in the USDA classification, with predominant medium to fine textures, high fragility, low cohesion, and shallow humic layers (IGAC, 1979). Soil bulk density is close to 0.8 Mg m -3 . Soils in the top 20 cm are moderately acid (pH H20 = 5.1), rich in soil organic matter (C = 50 mg g -1 ), low in base saturation (57%) and effective CEC (6.0 cmol kg -1 ), and also low P availability (Bray-II P = 4.6 mg kg -1 ). Low soil P availability is the result of high allophane content (52-70 g kg -1 ) which increases soil P sorbing capacity (Gijsman and Sanz, 1998).Experiments were set up at two locations in the Cauca Department hillsides on degraded soils previously cultivated with cassava for three years. Experiment BM1 was established at San Isidro Farm in Pescador. It was established as a random complete block (RCB) design with four treatments and three field replications. Treatments included two tree legumes, Indigofera constricta (IND) and Calliandra calothyrsus (CAL), one shrub, Tithonia diversifolia (TTH), and a natural regeneration or fallow (NAT). Plant species were selected on the basis of their adaptation to the hillside environment, ability to withstand periodical prunings, and the contrasting chemical composition of their tissues. The plot size was 18 m by 9 m. Experiment BM2 was established at the Benizio Velazco Farm also in Pescador. It was also established as a RCB design with three treatments due to limited space available and three field replications. Treatments included IND, CAL and NAT with same plot size and management as in BM1. Glasshouse grown two-month old Indigofera and Calliandra plants, inoculated with rhizobium strains CIAT 5071 and CIAT 4910 respectively and a common Acaulospora longula mycorrhizal strain, were planted in the field at 1.5 x 1.5 m spacing for treatments IND and CAL respectively. Tithonia cuttings were initially rooted in plastic bags before transplanting to the field using a 0.5 x 0.5 m spacing. During the first two months all planted fallows were frequently weeded to facilitate rapid establishment, thereafter no additional weeding took place. The natural regeneration treatment, NAT, received no management at all and served as control since this is the common practice of local farmers once their soils have become unproductive. Treatments IND and CAL were pruned to 1.5 m height at 18 months after planting and weighed biomass was laid down on the soil surface. In the TTH treatment, plants were pruned to 20 cm six times, starting six months after planting, and weighed biomass laid on the soil surface. Pruning intensity in TTH was guided by farmers concern that this common weed may become too competitive if allowed to produce seeds. In the case of IND and CAL the strategy was to reduce the impact of prunings on stem diameter increase and thus value as fuel wood at the end of the fallow phase. Whole plot measurement of biomass production during each pruning event was carried out and a composited subsample taken for laboratory analyses before laying down the pruned biomass on the soil surface. All above-ground biomass was harvested after 27 months with the conclusion of the fallow phase and left on the soil surface until soil sampling.Subsamples of each plant material evaluated were analyzed for total carbon (C), nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg). All plant material was ground and passed through a 1 mm mesh before analysis. C, N and P were determined with an autoanalyzer . Potassium, Ca and Mg were determined by wet digestion with nitric-perchloric acid followed by atomic absorption spectrometry (CIAT, 1993).High soil variability has been identified as a major limitation to evaluation of soil management strategies because of the difficulty in finding significant treatment differences in the area of study. Several measures were taken to address this potential limitation including splitting field replications in half and treating them as subplots from the beginning of the experiment, grid sampling for a composite subplot sample, and using covariance analysis.Twenty-five samples were collected in a grid pattern and composited for each subplot at 0-5, 5-10 and 10-20 cm respectively after 12 and 28 months under the four fallow treatments. Plant litter on the soil surface was carefully removed before collecting the soil samples. Samples from each plot were air-dried, visible plant roots removed, and the samples gently crushed to pass through a 2-mm sieve.Whole soil was ground with a mortar and pestle to <0.3 mm and then analyzed for C, N, and P. Total organic C was determined by wet oxidation with acidified potassium dichromate and external heating followed by colorimetry (Anderson and Ingram, 1993). Total N and P whole soil were determined by digestion with concentrated sulfuric acid using selenium as a catalyst, followed by colorimetric determination with an autoanalyzer. Bray P and exchangeable K were extracted with Bray II solution followed by colorimetric and atomic absorption determination respectively. Exchangeable Ca and Mg, and Al were extracted with 1M KCl solution and determined as described before (CIAT, 1993). Nitrate and ammonium were extracted in 1M KCl solution and determined by colorimetry with an autoanalyzer. . Separate soil samples were taken from each field replication after 28 months to assess soil physical, chemical and biological parameters at the end of the fallow period. Soil bulk density was determined every 5 cm soil depth by using 50 mm long cores with 50 mm internal diameter (Blake and Hartge, 1986). Measurements for other physical parameters used similar cylinders as those indicated above. Hydraulic conductivity was measured on undisturbed core samples using a constant head of water (Klute and Dirksen, 1986). Air permeability was determined by measuring the rate of air flowing in a core sample equilibrated at a suction of 7.5 KPa, using a Daiki DIK-5001 apparatus. Residual porosity was calculated as percentage of porosity remaining in the soil after subjecting it to a 20 KPa confined pressure at a suction equivalent to field capacity (Hakansson, 1990). Soil samples for chemical analyses were taken at three soil depths (i.e. 0-5, 5-10 and 10-20 cm).Special attention was paid to the soil macrofauna communities (i.e. soil invertebrates larger than 2 mm) in BM1. The sampling was performed using the method recommended by the Tropical Soil Biology and Fertility Programme (TSBF) (Anderson and Ingram, 1993). In each fallow system and repetition two samples of 25 cm x 25 cm x 30 cm were taken at regular 5 m intervals. A metallic frame was used to isolate soil monoliths that were dug out with a spade and divided into 4 successive layers (i.e., litter, 0-10, 10-20, 20-30 cm). Each layer was then carefully hand-sorted in large trays and all macro-invertebrates seen with the naked eye were collected, counted, weighed and preserved in 75% alcohol, except for the earthworms which were previously fixed in 4% formalin for 2 or 3 days.In the laboratory, invertebrates were then identified into broad taxonomic units (Orders or Families), counted and further grouped in 7 larger units, i.e., earthworms (Oligochaeta), termites (Isoptera), ants (Hymenoptera), beetles (Coleoptera), spiders (Arachnida), millipedes (Myriapoda), and \"other invertebrates\". Density and biomass of each of these 7 major groups were determined in each slash/mulch fallow system. Biomass was expressed as fixed weight in alcohol, 19% lesser than live weight for earthworms and termites, 9% for ants, 11% for Coleoptera, 6% for Arachnida and Myriapoda and 13% for the \"other invertebrates\" (Decaëns et al., 1994).Analyses of variance (ANOVA) for plant biomass and nutrient data from BM1 and BM2 experiments were conducted to determine the impact of experimental site and management regime on planted fallow species. Covariance analyses were conducted on soil data from the BM1 and BM2 experiments to determine the effect of fallow systems on soil parameters. In the case when covariance analysis for a parameter showed no significance, the Tukey's Studentized Range Tests were used to compare treatment means; conversely, when covariance analysis for a parameter was significant, the General Linear Models Procedure of Least Square Means (LSM) was used to compare treatment means. ANOVA for soil physical parameters were used to compare treatment means at the end of the fallow period for BM1 and BM2 respectively. All statistical analyses were conducted using the SAS program (SAS Institute, 1990).Experimental sites were of the same soil type and had a similar recent cropping history as stated above; nevertheless, they showed differences in certain soil parameters probably as a result of previous differences in soil management. Soil at BM1 experimental site was generally more acid, had a lower total C, higher total P, and considerably higher Bray P and exchageable Al than soil at the BM2 experimental site (Table 1).Total biomass production of the different slash/mulch fallow systems evaluated was higher in BM1 than in BM2, independent of treatment (Fig. 1). In BM1 the order of total biomass production was TTH>IND,CAL,NAT, while in BM2 the order was CAL,IND>NAT. Published values for leguminous trees in different agroforestry systems indicate average annual additions of dry matter biomass up to 20 Mg ha -1 yr -1 (Young 1997). The highest total biomass production, 17.1 Mg ha -1 yr -1 , corresponded to T. diversifolia, and was likely a result of fast growth and ability to withstand coppicing about every three months. This value is comparable to the mean dry biomass production of 18.0 Mg ha -1 yr -1 for Leucaena leucocephala and greater than the 11.3 Mg ha -1 yr -1 reported for Senna siamea in alley cropping systems (Van der Mersch et al., 1993). The mean biomass production of C. calothyrsus was 9.8 Mg ha -1 yr -1 and 9.0 Mg ha -1 yr -1 for I. constricta. The natural fallow (NAT), which represents the traditional fallow practice by local farmers, was dominated by herbaceous plants like Panicum viscedellum Scribn, Emilia sonchifolia (L.) DC., Hyptis atrorubens Poit, Mellinis minutiflora Beauv, Richardia scabra L., Panicum laxum SW and Pteridium aracnoideum (Kaulf.) Mabon. (Zamorano, 2000), and showed the lowest mean biomass accumulation (5.5 Mg ha -1 yr -1 ). The difference observed in annual increments of dry matter production between IND and CAL as affected by experimental site suggests that I. constricta is more responsive to better soil conditions found in BM1 than C. calothyrsus while the latter is more tolerant to poorer soil conditions found in BM2. However, further multi-location testing of these species is needed to better define the environmental niches for these slash/mulch fallow species. The relative contributions of nutrients through slash/mulch fallow management, expressed as percent of control (NAT), were generally highest in TTH (Table 2). Relative N contributions were highest in BM2 for both CAL and IND compared to BM1. This is possibly a result of the considerably lower (i.e. 40%) total aboveground biomass production in NAT in BM2 compared to BM1, because actual N inputs values were similar for both species in both experiments (data not shown). Research on the impact of nutrient contributions to the soil through the application of organic materials usually focus on N, increasingly on P, and least frequently on K, Ca or Mg. Nitrogen contributions through prunings of L. leucocephala and S. siamea in alley cropping systems were shown to contribute 307 kg ha -1 and 197 kg ha -1 respectively (Van der Mersch et al., 1993). Nitrogen contributions through slash/mulch systems TTH, IND and CAL in this study were 36%, 5% and 0.5% higher than for the L. leucocephala alley cropping systems mentioned above. Published values indicate that leguminous trees in alley cropping systems can contribute as much as 358 kg N, 28 kg P, 232 kg K, 144 kg Ca and 60 kg Mg per hectare (Palm, 1995). Nevertheless, nutrient availability in the soil is regulated to a large extent by the chemical composition or quality of plant tissues because they affect the rates of decomposition and nutrient release (Cadisch and Giller, 1997). All species used in this experiment have a N content greater than 2.5% which has been suggested as a conceptual threshold for N mineralization resulting in increased soil N availability to arable crops within a growing season (Palm et al., 2001). Nevertheless, while T. diversifolia and I. constricta decompose quickly because of their low lignin (6.9%, 4.6% respectively) and polyphenol (8.6%, 8.7%) contents and high in vitro dry matter digestibility (IVDMD) (72.4%, 77.4%), decomposition is slower in C. calothyrsus because of high lignin (14.5%) and polyphenol (18.4%) contents and low IVDMD (28.1%) (Cobo et al., 2002a). Recent studies also showed that fast decomposing, high quality plant materials (i.e. IND, TTH) generated high short-term N availability but low crop uptake; while slow decomposing, lower quality plant materials (i.e. CAL) resulted in greater N crop uptake presumably as a result of improved synchrony between soil nutrient availability and crop demand (Cobo et al., 2002b). Additional benefits from slash/mulch fallow systems include the contribution to soil nutrient pools from fine roots through root turnover and root dieback caused by pruning of above ground biomass. The importance of fine root and mycorrhiza turnover has generally been under emphasized as it has been shown in forest systems that they can contribute up to 4 times more N and up to ten times more P than above ground litterfall (Bowen, 1984). There is little information on the amount of nutrients supplied through roots in agroforestry systems (Palm, 1995). Root biomas of trees is usually between 20-50% of aboveground biomass, giving shoot:root ratios ranging from 4:1 to 1.5:1, but the proportion of roots becomes higher on nutrient-and/or water-limited soils (Young, 1997). Fig. 1. Dry matter aboveground production by slash/mulch and natural fallow systems at the BM1 and BM2 sites after 27 months.One important difference between slash/mulch fallow systems and biomass transfer systems is related to their long-term impact and sustainability. Slash/mulch fallow systems are likely to promote soil nutrient availability through remobilization of nutrients from less available soil nutrient pools. This may be a result of priming effects on soil mineralization processes triggered by labile C added with prunings as well as by root death and decomposition following slash/mulch. A considerable proportion of nutrients released is likely to be reabsorbed by the standing root biomass of fallow species and lead to new biomass growth. This cycle repeats with each slash/mulch event as nutrient recycling constitutes the basis of the functioning and sustainability of this cropping system. On the other hand, biomass transfer systems lead to variable levels of nutrient mining because they generate negative nutrient balances in soils under hedges and thus their long term use is limited as indicated by Gachengo et al. (1999) and Jama et al. (2000). Soil parameters showing significant differences among treatments included total N, available N (nitrate), exchangeable K, Mg, and Al for BM1 and available N (amonium, nitrate), and exchangeable K and Ca for BM2 (Table 3). Significant differences for most parameters, however, occurred after 12 or 28 months. The only parameters showing consistent significance across fallow age were total N in BM1 and exchangeable K in both BM1 and BM2. Because of high spatial variability, which is the characteristic feature of these hillside soils, significant changes are of considerable importance. Treatments means for soil parameters indicated are presented in Tables 4 and 5. Total soil N was highest (P < 0.05) in TTH, and CAL showed the second highest value after12 and 28 months of fallow duration (Table 4). After 12 months, NAT presented the lowest soil total N while IND had the lowest soil total N at the end of the fallow period (28 months). The beneficial effects of T. diversifolia on soil nutrients observed in the present study confirm previous published results of Gachengo et al. (1999), also on P-fixing soils. T. diversifolia is highly effective in scavenging soil nutrients as previously reported by Jama et al. (2000). This may be a result of profuse rooting systems in association with native mycorrhizae as well as the capacity to stimulate mineralization of adsorbed P and utilize organic phosphorus. C. calothyrsus and I. constricta, on the other hand, are both N-fixers deriving respectively 37 and 42 % of their N from the atmosphere (CIAT, 1996b). For each parameter only treatment means are presented when their effect was shown significant in Table 3 After 12 months, slash/mulch fallow systems containing TTH showed the highest exchangeable K and lowest exchangeable Al (P < 0.05) in BM1 (Table 5). Studies in acid soils of Burundi have also found a reduction in exchangeable Al by green manure additions, suggesting complexing of Al by organic materials (Young, 1997). In BM2 highest exchangeable K and Ca values were found in IND and NAT respectively. At the end of the fallow phase (28 months), exchangeable K was highest for TTH overall, but the trend for the common treatments among BM1 and BM2 was the same, with NAT and IND contributing significantly (P < 0.05) more than CAL. Exchangeable Mg in BM1 showed the same trend as K with the difference that the IND fallow system led to the lowest soil values. The high concentration of cations, especially K in T. diversifolia biomass (Table 2), and the pruning management in TTH is likely to be responsible for the highest contribution to soil exchangeable cations by this slash/mulch fallow system.The lack of significant changes in soil P parameters as a result of the slash/mulch fallow systems evaluated may, however, be influenced by the relative low amounts of P added to the soil compared with other nutrients like N and K (Palm et al., 1995) and also could be due to soils with a high P-sorption capacity (Rao et al., 1999). For each parameter only treatment means are presented when their effect was shown significant in Table 3 Soil fractionation generally increases the capacity to detect soil changes in SOM as a result of treatment compared to bulk soil measures (Barrios et al., 1996;1997). Recent results from Phiri et al. (2001) focusing on soil organic matter (SOM) (Meijboom et al., 1995) and P fractions (Tiessen and Moir, 1993), rather than conventional chemical analyses (e.g., Bray II P), indicate significant differences among treatments in experiment BM1 after 12 months. The slash/mulch fallow species in TTH, IND and CAL had an overall positive effect on soil fertility parameters when compared with the natural unmanaged fallow (NAT). T. diversifolia showed the greatest potential to improve SOM, nutrient availability, and P cycling because of its ability to accumulate high amounts of biomass and nutrients. The amount of P in the light (LL) and medium (LM) fractions of SOM correlated well with the amount of \"readily available\" P in the soil (Fig. 2). It is suggested that the amount of P in the LL and LM fractions of SOM could serve as sensitive indicators of \"readily available\" and \"readily mineralizable\" soil-P pools, respectively, in the volcanic-ash soils studied.Bulk density values reported for BM1 and BM2 are relatively low and are in agreement with published values for other volcanic ash soils (Shoji et al., 1993). After 28 months of fallow with the four systems, significant differences (P < 0.05) in bulk density were only found for the 0-5 cm soil depth of experiment BM2 (Table 6). While CAL and NAT were not different, IND showed significantly higher bulk density values (Table 7). A parameter showing significant treatment effects can result from low random error (i.e. bulk density) or a large separation of treatment means (i.e. air permeability) (Mead et al., 1993). The increased bulk density observed could be the result of a decrease in SOM levels. Although SOM levels in IND were lowest but not statistically significant (data not shown) in BM2, significantly lowest (P < 0.05) total N values were found in IND compared to other system treatment for BM1 (Table 4). Since soil total C and soil total N are highly correlated (Wild, 1988) we can assume that the I. constricta slash/mulch fallow generally promoted a reduction in SOM resulting in an increased soil bulk density. Fig. 2. The relationship between P content in the light (LL) and intermediate (LM) soil organic matter (SOM) fractions and sodium bicarbonate (NaHCO3) extractable organic P (A) and inorganic P (B) at BM1 after 12 months. The asterisk (*) indicates significance at a = 0.05.Soil air permeability was sensitive to treatment differences in BM1 (Table 6). This parameter measures the resistance of soil to air-flow and is associated to bulk density and hydraulic conductivity. While TTH showed the highest values, CAL and NAT showed intermediate values and IND the lowest values (Table 7). These results indicate that TTH improved structural stability of surface soil presumably as a result of changes in pore size distribution which allowed better air flow while IND led to greater resistance to air flow than the control NAT. The characterization of the soil macrofauna communities after 28 months of slash/much fallow treatments in BM1 showed taxonomically and functionally diverse taxa. A total of 22 taxonomic units (TU) were found. Macro-invertebrate total density ranged from 376.8 individuals (ind.) m -2 in TTH to 304.8 ind. m -2 in CAL. Conversely, macro-invertebrate biomass ranged from 18.2 g m -2 in IND to 6.1 g m -2 in TTH (Fig. 3). Other invertebrates corresponded to some nematodes (Mermithidae), hemipterans (Hemiptera) , snails (Gastropoda) and grasshoppers (Orthoptera). Termites (Isoptera) were almost absent from all fallow treatments, being less than 1% of total macro-fauna abundance. For each parameter only treatment means are presented when their effect was shown significant in Table 6 The main groups of soil macro-invertebrates were rather abundant, especially ants (Hymenoptera). The abundance of ants, comprised of several species, was highest in TTH (254.8 ind.m -2 ) and lower in IND and NAT (176 ind.m -2 ). Earthworm density was lowest in TTH (19.2 ind.m -2 ) and highest in IND (106.8 ind.m -2 ). These two taxa were the main components of total macro-invertebrate biomass in all systems ranging from 46.9% in TTH to 73.1% in IND in the case of earthworm biomass. We found the exotic earthworm species Pontoscolex corethrurus (Glossoscolecidae) that is commonly found when tropical natural ecosystems are replaced by different production systems (Fragoso et al., 1999). In some Amazonian agroecosystems, the presence of this exotic has had a negative effect on soil properties, mainly due to loss of the original earthworm diversity rather than to the mere presence of this earthworm (Chauvel et al., 1999). Larvae of beetles (Coleoptera) were also highly abundant and their biomass was lowest in IND (78.8 g m -2 ) and highest in TTH (120.8 gm -2 ). Fig. 3. Density and biomass of soil macroinvertebrate communities in the slash/mulch fallow systems at the BM1 site at the end of the fallow phase.The impact of slash/mulch treatment differences is consistent with other results presented and suggest three generally distinct groups TTH, CAL+NAT, and IND. High ant activity in TTH, as indicated by high density, suggests that we may be underestimating the potential impact of T. diversifolia additions because a considerable proportion may be exported by ants to their nests. On the other hand, earthworm activity is well known for stimulating N mineralization rates (Barois et al., 1987;Lavelle et al., 1992;Decaëns et al., 1999;Rangel et al., 1999) and the observation of particularly high numbers of individuals in IND coincides with the observation of a reduction in total soil N and an increase in soil available N. The conspicuous presence of P. corethrurus in IND and the observation that compact casts increase soil compaction (Hallaire et al., 2000), because of the limited presence or absence of soil fauna able to decompact such casts (Blanchart et al., 1997, P.Lavelle pers.comm.), suggests that increased soil bulk density observed in IND may have been mediated by increased activity of this species.Some groups of soil macroinvertebrates may have beneficial effects on some soil parameters evaluated but others, on the contrary, may cause damage since they constitute soilborne pests. Therefore, it is necessary to increase the level of resolution of identifications studies to the species level. This seems to be of particular relevance when using soil macrofauna as biological indicators of soil functioning and health (Pankhurst et al., 1997). Nevertheless, since information on soil fauna was not available at the beginning of the experiment and was limited to BM1, conclusions regarding the impact of production systems on the soil macrofauna communities must be considered preliminary.Slash/mulch planted fallow systems evaluated in this study were more productive in terms of biomass production and nutrient recycling than the traditional practice of natural regeneration by native flora, suggesting that the objective of increased nutrient recycling was achieved. This study attempted to integrate understanding of the impacts of slash/mulch planted fallow systems on soil quality by simultaneously evaluating the chemical, physical and biological dimensions of the soil. The TTH slash/mulch fallow system proved to be the best option to recover the overall soil fertility of degraded soils following cassava monocropping in the study area. Nevertheless, its use may be limited in areas with seasonal drought as it is not very tolerant to extended dry periods. The CAL slash/mulch fallow system proved to be the most resilient as it produced similar amounts of biomass independent of initial soil quality and thus a candidate for wider testing as a potential source of nutrient additions to the soil and fuelwood for rural communities. The slower rates of decomposition in CAL, compared to IND and TTH, suggest that benefits provided may be longer lasting and potential losses would be reduced through improved synchronization between nutrient availability and crop demand. The IND slash/mulch fallow, on the other hand, showed more susceptibility to initial soil quality and this may limit its potential for extended use.Increased soil bulk density as a result of decrease in SOM, observed in slash/mulch planted fallows using IND, was possibly mediated by the presence of large populations of the endogeic earthworm P. corethrurus. This earthworm species is known to stimulate N mineralization and to be responsible for soil compaction when a diverse macrofauna community capable of ameliorating soil physical structure is limited or absent. Although increased available N may have positive short-term impacts, the significant decrease in total soil N suggests that considerable N losses may be occurring during the fallow phase and benefits to subsequent cropping could be limited. Further multilocation testing is needed to confirm these observations, and also to study the 'fallow effect' on crop yield as well as the economic feasibility of slash-mulch fallow systems.Phosphorus (P) is an essential nutrient for plants and often the first limiting element in acid tropical soils. Profound understanding of the P dynamics in the soil/plant system and especially of the short-and long-term fate of P fertilizer in relation to different management practices is essential for the sustainable management of tropical agroecosystems (Friesen et al., 1997). Chemical sequential extraction procedures have been and still are widely used to divide extractable soil P into different inorganic and organic fractions (Chang and Jackson, 1957;Bowman and Cole, 1978;Hedley et al., 1982;Cross and Schlesinger, 1995). The underlying assumption in these approaches is that readily available soil P is removed first with mild extractants, while less available or plant-unavailable P can only be extracted with stronger acids and alkali. In the fractionation procedure developed by Hedley et al. (1982) and modified by Tiessen and Moir (1993), the P fractions (in order of extraction) are interpreted as follows. Resin-P i represents inorganic P (P i ) either from the soil solution or weakly adsorbed on (oxy)hydroxides or carbonates (Mattingly, 1975). Sodium bicarbonate 0.5 M at pH 8.5 also extracts weakly adsorbed P i (Hedley et al., 1982) and easily hydrolysable organic P (P o )-compounds like ribonucleic acids and glycerophosphate (Bowman and Cole, 1978). Sodium hydroxide 0.5 M extracts P i associated with amorphous and crystalline Al and Fe (oxy)hydroxides and clay minerals and P o associated with organic compounds (fulvic and humic acids). Hydrochloric acid 1 M extracts P i associated with apatite or octacalcium P (Frossard et al., 1995). Hot concentrated HCl extracts P i and P o from more stable pools. Organic P extracted at this step may also come from particulate organic matter (Tiessen and Moir, 1993). Residual P, i.e. P that remains after extracting the soil with the already cited extractants, most likely contains very recalcitrant P i and P o forms.Several studies related these different P fractions in tropical soils to plant growth (Crews, 1996;Guo and Yost, 1998) or showed the influence of land-use and the fate of applied fertilizers (Iyamuremye et al., 1996;Linquist et al., 1997;Lilienfein et al, 1999;Oberson et al., 1999), and partly resulted in contrasting assignments of fractions to pools of different availability. By comparing the amounts of P extracted from the surface horizons of Brazilian Oxisols that had been under different land-use systems for 9-20 years, either unfertilized or with mineral P fertilizer application, Lilienfein et al. (1999) showed that most of the fertilizer was recovered in the Bic-and NaOH-P i fractions, irrespective of the land-use system (resin-P i was not measured). In a 4-year field study conducted on a Hawaiian Ultisol, Linquist et al. (1997) recovered one year after fertilizer application almost 40% of the applied triple super phosphate (TSP) fertilizer in the hot HCl and H 2 SO 4 fractions. Oberson et al. (1999) showed that in an Oxisol managed as a legume-grass pasture for 15 years resin-P i , Bic-and NaOH-P i as well as NaOH-P o levels were maintained at a higher level over the whole year in comparison to the same soil with the same total P content but managed as a grass only pasture. Iyamuremye et al. (1996) found an increase of resin-P i , Bic-P i and -P o as well as NaOH-P i after addition of manure or alfalfa residues to acid low-P soils from Rwanda. In the study of Guo and Yost (1998), resin-P i , Bic-and NaOH-P i were most depleted by plant uptake on highly weathered soils. NaOH-P i was important in buffering available P supply while significant depletion of organic fractions could rarely be measured.A possible method to gain information about the availability of different P fractions is to label soil P, fertilizers or plant residues before applying the sequential fractionation scheme (MacKenzie, 1962;Weir andSoper, 1962, Dunbar andBaker, 1965). Two studies followed the movement of labeled P from plant residues to soil P fractions applying a modified Hedley (Daroub et al., 2000) or the Chang and Jackson (1957) fractionation procedures (Friesen and Blair, 1988). They found that at six or eleven days, respectively, after plant residue addition between 20 and 50 % of the label was extractable as P i with a resin (Daroub et al., 2000) or with NH 4 Cl and NH 4 F (Friesen and Blair, 1988). For longer incubation periods up to 34 days, Daroub et al. (2000) showed a subsequent movement of the label from the resin-P i fraction to the NaOH-P i fraction. The results obtained in these studies suggest that, in tropical soils, the amounts of P in the different pools measured by sequential P extraction procedures and the fluxes of P between pools are controlled both by physico chemical factors (sorption/desorption) and by biological reactions (immobilization/mineralization). However, the importance of these different reactions for different land-use systems, such as monocropping, pasture or intercropping, remain largely unknown.The objective of this study was to assess the effect of different land-use systems (native savanna, rice monocropping, rice green manure rotation, grass legume pasture) on some physico chemical and biological reactions involved in P cycling in a Colombian Oxisol. Surface soil sampled in the different cropping systems was labeled with carrier-free radioactive P ( 33 P). After various incubation times, P was sequentially extracted by the modified Hedley procedure (Tiessen and Moir, 1993) and 31 P and 33 P were measured in each fraction.Soils included in the study were sampled during the rainy season in September 1997 from a field experiment (Friesen et al., 1997) 1).The surface soil layer (0-20 cm) was sampled in the long-term \"Culticore\" field experiment, which was established in 1993 with the objective to test the effect of different farming systems on plant productivity and soil fertility (Friesen et al., 1997). The experiment had a split-plot design with four replicates with treatment sub-plots of 0.36 ha size. The soil samples used for this study were taken at random in two replicates of each treatment and the replicates were mixed for the laboratory analysis. For our study, the following treatments were included.1 grown in monoculture; one crop per year followed by a weedy fallow incorporated with early land preparation at the beginning of the rainy season before sowing rice. 4. RGM (Rice green manure rotation): Rice followed by cowpea (Vigna unguiculata, var. ICA Menegua) in the same year. The legume was incorporated at the maximum standing biomass level in the late rainy season before sowing rice in the following rainy season.Before establishing the treatments, GL, CR, and RGM on savanna, the soil was conventionally tilled after burning the native vegetation. At the beginning of the experiment all treatments except SAV were limed using 500 kg dolomitic lime ha -1 . Fertilization of rice was 80 kg N ha year -1 (urea, divided among three applications), 60 kg P ha year -1 (triple superphosphate), 99 kg K as KCl, 15 kg Mg and 20 kg S (as MgSO 4 ) and 10 kg Zn ha -1 at establishment and according to plant needs afterwards. With cowpea additionally 20 kg N and 40 kg P ha year -1 and 60 kg K, 10 kg Mg, 13 kg S and 10 kg Zn ha -1 at establishment and in adequate rates afterwards were applied. The introduced pasture (GL) received additional fertilization only in 1996 (per ha: 20 kg P, 20 kg Ca (lime), 10 kg Mg (lime), 10 kg S (elemental) and 50 kg K (KCl)). Phosphorus input-output balances were estimated by subtracting the P removed from the system by grain and/or with animal live weight gains from the P applied in mineral fertilizers. Phosphorus exports in grain were calculated by multiplying weighed rice grain yields with measured P contents in grains. P exported in the animals was assumed to be 8 g per kg of live weight gain. Live weight gains in GL were on average 68 kg ha -1 yr -1 (Oberson et al., 2001). Cultivated soils were tilled to a maximum of 15 cm depth.Topsoil samples (0-20 cm) were air-dried and sieved at 2 mm before they were used for chemical analysis in the analytical service laboratory of CIAT or shipped to Switzerland where they were stored in air-dried condition until use for the fractionation experiment in 2000.Bray-II P was extracted using dilute acid fluoride (0.03 M NH 4 F, 0.1 M HCl) at 1:7 soil solution ratio using 2 g soil and 40 sec shaking time. Total soil P (P tot ) was determined on samples of 0.25 mg soil with addition of 5 mL concentrated H 2 SO 4 and heating samples to 360° on a digestion block with subsequent stepwise (0.5 mL) additions of H 2 O 2 until the solution was clear (Thomas et al., 1967). letter in a column shows that no significant differences were observed between the treatments Microbial P, C and N (P Chl , C Chl and N Chl ) were determined on the same moist, preincubated samples as for the sequential P fractionation by extraction, of chloroforme fumigated and unfumigated samples, with Bray I (0.03 M NH 4 F, 0.025 M HCl) (P Chl ) (Oberson et al., 1997) or K 2 SO 4 (C Chl and N Chl ) (Vanceet al., 1987). No k-factors (Brookes et al., 1982;Hedley and Stewart, 1982;McLaughlin et al., 1986) were used to calculate P mic , C mic or N mic from measured P Chl , C Chl and N Chl as there exist no proper estimates for these acid tropical soils (Gijsman et al., 1997). P Chl was corrected for sorption of released P according to Oberson et al. (1997). Dithionite-citrate-bicarbonate extractable and oxalate extractable Fe and Al (Fe d , Fe ox , Al d , Al ox ) were determined according to Mehra and Jackson (1960) and McKeague and Day (1966). The mineralogy of the soils was determined on total soil samples, pretreated with H 2 O 2 to remove organic C, using X-ray diffraction analysis (XRD) (Table 1). The samples were ground under acetone in a tungsten carbide vessel of a vibratory disk mill (Retsch RS1) for 10 minutes. Longer grinding times were not applied due to the detrimental effect that further grinding can have on the crystallinity of minerals, especially Fe (hydr)oxides (Weidler et al., 1998). For the Cu Kα, the Bragg-Brentano geometry was chosen as an XRD routine setup. The measurement were carried out on a Scintag XDS 2000 equipped with a solid state detector from 2 to 52 °2 \\θ with steps of 0.05 °2\\θ and counting times of 16 seconds.Before starting the sequential P fractionation, the soils were preincubated in a climate chamber (24°C and 65 % relative atmospheric humidity, no light) for two weeks in portions of 100 g at 50% of their water holding capacity (300 g water kg -1 soil dry weight). Soil water content was controlled and adjusted every other day by weighing.Subsamples of preincubated soils were labeled in portions of 15 g with 120 MBq 33 P kg -1 which were added with 10 μl deionized water per g soil. The mass of P introduced with the 33 P label can be neglected (<2.5 x 10 -3 g P g -1 soil, Amersham product specification, July 2000). Therefore, the term 'P concentration' always refers to 31 P and specific activities (SA) are calculated as: SA (Bq g -1 P)= 33 P/ 31 P [Eq. 1]Soil P was fractionated sequentially with three replicates per soil following the modified method of Hedley et al. (1982), as described in Tiessen and Moir (1993), with HCO 3 -saturated resin strips (BDH # 55164, 9 x 62 mm), followed by 0.5 M NaHCO 3 (referred to as Bic-P), 0.1 M NaOH, (these first three steps each with an extracting time of 16 h) and concentrated hot HCl at 80° C for 10 minutes. The step using diluted cold HCl was omitted, as Ca-phosphates are only present at very low levels or are absent in highly weathered acidic soils (Agbenin and Tiessen, 1995), as shown for the soils used in this study by Friesen et al. (1997). Residual P was extracted as described previously for determination of P tot .The amount of soil extracted was doubled from 0.5 to 1 g using the original volumes of extractants (2 resin strips in 30 mL H 2 O, 30 mL NaHCO 3 , 30 mL NaOH, 15 mL concentrated HCl, 5 mL conc. H 2 SO 4 ) in order to get higher 33 P-concentrations in the extracts. This was preferred to the alternative of higher label application as the radiation might affect microbes (Halpern and Stöcklin, 1977). After each extraction, the samples were centrifuged at 25000 x g for 10 minutes before filtering the solutions of the Bic-and the NaOH-extraction through 0.45 m pore size millipore filters (Sartorius, cellulose acetate), and the hot HCl and residual P extract through a Whatman filter Nr. 40.Phosphorus concentration in all extracts was measured after neutralization by the Murphy and Riley (1962) method. This method was used directly, after neutralization of the extracts, for the P recovered from the resin strip and for P i determination in the HCl extract. Organic matter was first precipitated by acidification in the Bic-and the NaOH-extracts prior to P i determination (Tiessen and Moir, 1993). Total P (P t ) in the Bic-, the NaOH-and the HCl-extracts was measured after digestion of P o with potassium persulfate (Bowman, 1989). Organic P was calculated as the difference between total P and P i in the Bic-, NaOH-and hot HCl extracts.To partition soluble 33 P i and 33 P o in the Bic-, the NaOH-and the hot HCl-extracts into separate solutions before counting, 5 mL of the extracts were shaken with acidified ammonium molybdate dissolved in isobutanol (Jayachandran et al., 1992). With this method, P i is extracted into the isobutanol while P o remains in the aqueous phase. The complete recovery of P i in the isobutanol phase was verified with the addition of a standard amount of 33 P in 0.5 M HCO 3 , 0.1 M NaOH and in 2.3 M HCl; recovery rates of added 33 P in the isobutanol phase were between 97 % and 103 %, which was not significantly different from 100%. Counts in the aqueous phase were 1.1 % (HCO 3 ), 0.3 % (NaOH) and 0.1 % (HCl) of the original solutions showing that hardly any P i goes into this phase. Determination of total P in the aqueous phase is not possible because the presence of the molybdate interferes with the analysis (Jayachandran et al., 1992).The radioactivity in each phase was determined with a liquid scintillation analyzer (Packard 2500 TR) using Packard Ultima Gold scintillation liquid in the ratio (extract to liquid) 1:5. The values were corrected for radioactive decay back to the day of soil labeling. All extracts were tested for possible quenching effects by adding defined 33 P spikes. Quenching in the acid resin eluate could be prevented by dilution of 250 l eluate with 750 l deionized water for counting. The quench effect in the hot concentrated HCl extract could be avoided by counting in the solutions separated with acidified isobutanol because the separated phases were not affected by quenching. All other extracts were not affected by quench effects.The recovery of the label as sum of all fractions, including residual P, was never complete. Therefore, subsamples of the soil residue after final acid digestion were dried and weighed into scintillation vials. These subsamples were then counted after addition of 1 mL water and 5 mL of scintillation cocktail.The procedure of isotopic exchange kinetics was used to assess the exchangeability of P i in the soils sampled in the different land-use systems. The method was conducted as described by Fardeau (1996). Suspensions of 10 g of soil and 99 mL deionized water were shaken for 16 h on an overhead shaker to reach a steady state equilibrium for P i . Then, at t = 0, 1 mL of carrier free H 3 33 PO 4 tracer solution containing 1.2 MBq was added to each continuously stirred soil water suspension. Three subsamples were taken from each sample after 1, 10 and 100 minutes, immediately filtered through a 0.2 μm pore size micropore filter, and the radioactivity in solution was measured by liquid scintillation as described previously. To determine the 31 P concentration in the soil solution (C p , mg P L -1 ) 10 mL of the solution were filtered through a 0.025 μm filter (Schleicher & Schuell, NC 03) at the end of the experiment. The smaller filter pore size was used to exclude any influence of suspended soil colloids on C p determination (Sinaj et al., 1998). The P concentration in the filtrate was measured in a 1 cm cell using the Malachite green method (Ohno and Zibilske, 1991) with a Shimadzu UV-1601 spectrophotometer. As the concentrations in the solutions of SAV and GL were close to the detection limit, they were additionally measured in samples concentrated by evaporation (5:1). This procedure resulted in C p values that were not significantly different from the non-concentrated solutions.Assuming that at any given exchange time the specific activity (SA) of inorganic phosphate in the solution is equal to the SA of the total quantity of phosphate which has been isotopically exchanged, it is possible to calculate the amount of isotopically exchanged P (E t , mg P kg -1 soil). The amount of P exchangeable within one minute (E 1 ), indicating the immediately available P, is expressed as (Fardeau, 1996):where R is the introduced radioactivity and r 1 is the radioactivity remaining in solution after 1 minute of isotopic exchange. The factor 10 results from the soil solution ratio of 1:10.The effects of land-use systems and incubation time after labeling on P fraction size were tested by two-way ANOVA and Tukey's multiple range over all treatments and times of fractionation. A separate one-way ANOVA was used to test the difference on label recovery and fraction size between samples labeled in soil water ratio 1:10 and samples labeled in incubated moist state 4 hours after labeling. Percentage recovery data were log-transformed to meet the requirements of analysis of variance. Time and soil treatment influences on the Sas of each fraction were tested by a two-way ANOVA and, as the interaction time X treatment was significant for all fractions, the treatment influence was tested for each repitition in time of sequential fractionation, separately.The mineralogy and the Fe and Al (oxy)hydroxides contents of the surface soil from the four treatments was normal for this type of soil (Gaviria, 1993). On average of all treatments, the soil contained 68% quartz, 23% kaolinite, 4% anatase, 3% gibbsite, 2% rutile, and <1% vermiculite. There were no significant differences among the different land-use systems (SAV, GL, CR, RGM). This implies that any difference seen in the P dynamics among land-use systems was mainly due to the land-use system and not to differences in the soil mineralogy.The amounts of total P directly extracted from the soil samples (P tot ) were not significantly different from the sum of P (P sum ) extracted in the different fractions of the sequential extraction for SAV and CR while the direct extraction led to significantly higher values (P<0.05) for GL and RGM (Table 2). To evaluate whether differences in total P content in soils were related to P fertilization, the increase in P tot content (calculated as the difference between total P extracted from fertilized GL, CR or RGM) and P tot extracted from non fertilized SAV was compared to the estimated P balance of these treatments (significant correlation, r 2 =0.87; P<0.001). The increases in P tot were of the same order of magnitude as the calculated P balance. Given the imprecision of the methods used to determine total P contents (O'Halloran, 1993) and of the estimations made to calculate the P balance, these results suggest that most of the P added with fertilizers and not taken up by plants remained in the surface layers of the studied soils. Except for the CR soil these results agree well with Oberson et al. (2001). In their study only about half of the calculated positive P balance was recovered in total P. The sampling depth of 0-10 cm might explain this difference: soil tillage may have mixed P in the 0-10 cm soil layer with soil in the 10-20 cm layer, resulting in incomplete recovery of P in the 0-10 cm sampling depth. The effect of the four land-use systems on P i exchangeability in the surface layer of the studied soil is presented in Table 3. The ratio r 1 /R, which is inversely correlated to the P sorbing capacity of soils (Frossard et al., 1993), was below 0.05 for all treatments suggesting that these soils have a high P sorbing capacity (Frossard et al., 1993). Furthermore, the r 1 /R-values of the four treatments were positively correlated with the directly extracted total soil P (r 2 =0.76 P<0.001). This suggests that the different landuse systems have resulted, through their different P fertilization and cropping, in different sorption rates of P i on soil minerals. Since in Oxisols P sorption is governed by the Al and Fe (oxy)hydroxides, these treatments probably induced different degree of P i saturation on the soil metallic (oxy)hydroxides such as gibbsite, which was identified in the soil from these treatments.The P i concentration in the soil solution (C p ) was close to the detection limit in SAV, GL and CR treatments (Table 3). Although significantly different between all treatments, C p was significantly increased only in the RGM treatment (P<0.001). In SAV, GL and CR, C p was much lower than the critical concentration needed to sustain optimal growth for a large range of crops (Kamprath and Watson, 1980;Fox, 1981). The P i concentration in the soil solution was not correlated with the total soil P content. The clear C p increase in RGM was therefore not only due to an increase in total P but also to other mechanisms. The strong increase in soil biological activity observed in land-use systems including legumes might partly explain this higher C p value (Haynes and Mokolobate, 2001;Oberson et al., 2001). The variation in the amount of P i isotopically exchangeable in one minute (E 1 ) followed the same trend as the variation in C p . 1. § ratio of radioactivity remaining in soil solution to radioactivity added at time 0 after 1 minute of isotopic exchange. ¶ P concentration in the soil solution measured at soil:water ratio 1:10. # Quantity of P exchangeable within 1 minute.The positive P balances of the fertilized GL, CR and RGM treatments resulted in significantly higher P concentrations (P<0.001) compared to the savanna soil in all fractions except the organic fractions and residual P (Table 4). This agrees with the results of Friesen et al. (1997) and Oberson et al. (2001), who fractionated P forms according to the same method in the same field experiment, and studies conducted in other tropical soils (Beck and Sanchez, 1994;Linquist et al., 1997). Our results show that resin-P i , Bic-P i and NaOH-P i increased with P fertilizer input, with the NaOH-P i fraction being the main sink for the applied P. This P sink function of the NaOH-P i fraction can be explained by the adsorption of P i through ligand exchange with hydroxyl groups (Sposito, 1989) located on the surface of Fe and Al (oxy)hydroxides (Ainsworth et al., 1985;Parfitt, 1989;Torrent et al., 1992) and by the desorption of P i from the surface of (oxy)hydroxides in the presence of 0.5 M NaOH (Houmane et al., 1986;Cross and Schlesinger, 1995).During the continuous 2-week incubation of the soil samples, the resin and the Bic-P i fractions increased significantly (P<0.05) between the first and second fractionation date for all soils (between 4 and 14 mg kg -1 for the sum of resin and Bic-P i ). There was no significant and corresponding decrease in any fraction although total extractable P o tended to decline (between 8 and 18 mg kg -1 ) for all soils (Table 5). The absence of significant compensating movements of P out of P o fractions may be due to the high variability of the results, especially for the organic fractions where coefficients of variation for Bic-P o were between 13 and 70 % and for NaOH-P o between 7 and 45 %. Since P o is determined by the difference between P t and P i there are multiple sources of error. High variability of repeated measuring of Bic-and NaOH-P o were reported in Magid and Nielsen, (1992). Problems in the determination of P i are mentioned in Tiessen and Moir (1993), especially the possibility that P i is precipitated along with the organic matter upon acidification and erroneously determined as P o (P t -P i ). On the other hand, P o compounds could be hydrolyzed in the acidic solution during the measurement of P during the colorimetric essay (Condron et al., 1990;Gerke and Jungk, 1991).Increases in resin and Bic-P i between 4 hours and 1 week of incubation suggest that mineralization of P o led to the release of labile P i from P o fractions. As the first fractionation was started 4 hours after labeling, the disturbance by mixing the soil with the label and the momentarily increased humidity might additionally have stimulated the microbial activity despite of the preincubation. A temporary stimulation of the microbial activity by the thorough mixing when labeling soil was indicated in microbial turnover studies conducted on soils from the same field experiment (Oberson et al., 2001). This assumption seems likely, as there were little changes in fraction sizes between the second and the third fractionation indicating a stabilization of the system.The fraction of 33 P recovered in the resin-P i fraction 4 hours after labeling varied between 22 % in SAV and 60 % in RGM (Figure 1). The 33 P recovery in this fraction was positively correlated to the content of total P of the soils (r 2 =0.87; P<0.001, 4 h after labeling). The corresponding decrease of 33 P in the resin fraction in RGM and CR corresponded with an increase in label recovery in Bic-and NaOH-P i , while in SAV and GL the decline in resin 33 P was accompanied by an increase in 33 P in NaOH-P o (GL also NaOH-P i ), HCl-P i and residual-P. For SAV and GL, the label recovered in the resin-P i , and Bic-P i did not change much between the 1 st and the 2 nd week and the amount of 33 P in NaOH-P i was stable over the entire incubation time. This shows that in SAV and GL the label was rapidly exchanged between these fractions and that equilibrium with the (labeled) soil solution was reached. In contrast, 33 P in the Bic-P i and the NaOH-P i of CR and RGM was still increasing after one week while the resin-33 P i continued to decrease, showing that the exchange between these fractions was incomplete.The data for 33 P o were, because of the determination after the separation from Pi with the isobutanol method, not affected by the inherent problems in determination of the P o fractions in the Hedley fractionation scheme as described previously. Only small amounts of the label were found in organic fractions after 4 hours, but there were already significant differences in NaOH-33 Po (P<0.001) in the order: SAV (4%) ≈ GL (2%) > CR (0.4 %) ≈ RGM (0.1 %). This might be due to differences in microbial activity as observed by Oberson et al. (2001) in the same field experiment. Actually, the microbial biomass in incubated soils, indicated by measured P Chl , C Chl and N Chl values, was significantly different between the soils (Table 5), despite the fact that the samples had been stored in air-dried condition for more than three years before being used in this study. The assumption that recovery of the label in organic fractions was actually due to active processes and not to any analytical artifact is supported by the observed increases of NaOH-33 P o and HCl-33 P o for all soils over time. The total recovery of 20 % (SAV) or 14% (GL), respectively, of the label in organic fractions two weeks after labeling shows that these compartments have to be taken into account to understand the fate of P in these very low-P soils (Tiessen et al., 1984;Beck and Sanchez, 1994;Linquist et al, 1997). ‡ see Table 1.The proportion of label in the hot HCl and residual P fractions increased significantly with incubation time in all soils. This contradicts the prevailing opinion of recalcitrance of the P in these fractions (Guo and Yost, 1998;Neufeldt et al., 2000). While the total P content in the residual fraction varied significantly with time (Table 4), this was not the case for hot HCl extractable P i , while hot HCl extractable Po tended to decrease. This suggests that the movement of the label to these fractions was not due to net P-movement but to exchange processes.Total 33 At all sampling times during the incubation study, in total between 67 % and 94 % of the applied 33 P label could be recovered in the sum of all fractions (Fig. 1). This sum was generally in the order SAVBic-P i >NaOH-P i >HCl-P i >residual P), showing that the strongest reactants extracted either large quantities of slowly exchangeable P or a large quantity of P in which only a small part was rapidly exchangeable. After 2 weeks the SAs of resin-P i , Bic-P i and NaOH-P i became closer, suggesting that equilibrium with respect to P transfer between these fractions was being approached. The SAs of resin-P i , Bic-P t and NaOH-P i were not significantly different in SAV and GL while the SA of resin-P i was still significantly higher than the SA of Bic-P i and NaOH-P i in CR and RGM. These observations show that it is not possible to discuss the exchangeability of a certain P fraction without relation to a defined time of exchange (Fardeau et al., 1996).Although the SAs of the NaOH-P o and HCl-P o fraction were relatively low they showed that, depending on land-use, these fractions were connected through active processes with the soil solution, most probably through microbial activity (Oehl et al., 2001). This indicates that the determination of plant available P with short-term isotopic exchange experiments might lead to errors since the dynamics of organic P forms are excluded.The effect of contrasting land-use systems on the P fractions extracted by the sequential fractionation procedure was assessed in an Oxisol during a 2-week incubation on soils labeled with carrier free 33 P. The results show that in the studied Oxisol, the quantities of 31 P and 33 P recovered in the different fractions were strongly dependent on the total P content of the soil, which was determined by the amount of P added by fertilizers and by plant P uptake. In the two soils fertilized annually with P and with a large positive P input-output balance, most of the P i was stored in the resin-P i , Bic-P i and NaOH-P i fractions. The use of carrier free 33 P confirmed that, under all land-use systems studied, these soil P fractions contained most of the exchangeable P and that 33 P was transferred from the soil solution first to the resin fraction and then to the Bic-P i and NaOH-P i fraction. This suggests that, when this Oxisol is regularly fertilized, P is stored in these three fractions while the plants might take up P from the same fractions. In the two other soils, which had either never been fertilized or had been fertilized only once at the beginning of the field trial, the transfer of 33 P in these three fractions (i.e. resin-P i , Bic-P i and NaOH-P i ) was less clear, suggesting that the soil P i was much less exchangeable. In these soils, however, the transfer of 33 P into organic P fractions was more important (up to 20 % of the label was found in the organic P fractions two weeks after labeling). As the pool sizes of these organic fractions did not change significantly over time of incubation, the label recovery indicates relatively quick cycling processes, probably mostly of microbial P. In such low P soils, these processes are relevant and should be considered when estimating soil P availability for plants.Tropical savannas cover 45% of the land area in Latin America, or 243 million hectares (Mha), mainly in Brazil (200 Mha), Colombia (20 Mha), and Venezuela (12 Mha). The soils are mainly Oxisols and Ultisols, which are characterized by low nutrient reserves, high acidity (pH 4.0-4.8), high aluminum (Al) saturation (up to 90%), high phosphorus (P) fixing capacity (Sánchez & Logan, 1992), and a low capacity to supply P, K, Mg and S. In addition to soil chemical constraints, these soils also exhibit high bulk density, high resistance to root penetration, low rates of water infiltration, low water holding capacity, and low structural stability (Amézquita, 1998a, b;Phiri et al., 2001a). These chemical and physical constraints have to be alleviated in order to make these infertile soils productive and sustainable for agriculture.These soils have traditionally been used for extensive cattle ranching on native forage, dominated by Andropogon and Trachypogon grasses, with low management and almost no purchased inputs (Fisher et al., 1994). Native pasture productivity on these soils is correspondingly low.Land demand for intensive agricultural production on these soils has increased in the past 20 years. However, intensified agricultural production is usually constrained by poor soil chemical and physical properties. Traditional methods of cultivation by disc harrowing often lead to soil structural deterioration and erosion (Preciado, 1997;White, 1997 ). Research in the eastern plains (Llanos Orientales) of Colombia has shown that these soils are susceptible to physical, chemical, and biological degradation once brought into cultivation (Amézquita, 1998a, b). One of the effects of increasing land preparation is reduction in soil volume due to the decrease in size of soil aggregates. As a consequence, it causes changes in total porosity and pore-size distribution, affecting the flow of water and nutrients. Total porosity, water holding capacity, and macroporosity decline as cultivation is prolonged (McBratney et al., 1992;Preciado, 1997;Amézquita, 1998a). Plowing causes disruption of peds, and this exposes previously inaccessible organic matter to attack by microorganisms while the population of structure-stabilizing fungi and earthworms decrease markedly (White, 1997). These changes result in soil degradation, which reduces water infiltration and increases the loss of soil and plant available nutrients by soil erosion and surface runoff (Amézquita & Londoño, 1997;Amézquita & Molina, 2000).The practicality of rehabilitating degraded lands depends on the cost relative to the output or environmental benefits expected (Scherr & Yadav, 1996) and their influence on yields. The impact of soil degradation should be assessed in relation to critical limits to crop growth of key soil properties. Identification of appropriate methods of soil restoration is facilitated by knowledge of the key soil properties that influence soil quality and their critical limits in relation to the severity of soil degradation (Lal, 1997).To achieve improved and sustainable crop and pasture production and to avoid degradation, key soil properties such as soil's physical constraints must be alleviated by appropriate tillage and cropping practices (Amézquita, 1998a;Phiri et al., 2001a). A highly successful strategy for intensifying agricultural production in a sustainable manner and reversing problems of soil degradation involves the integration of crop-pasture systems (agropastoralism) (Vera et al., 1992;Rao et al., 1993;Thomas et al., 1995). This strategy is based on the assumption that a beneficial synergistic effect on production and on soil quality occurs when annual and perennial species are combined in time and space (Spain, 1990;Lal, 1991). Available nutrients are used more efficiently and the chemical, physical and biological properties of the soil are improved.Phosphorus is among the nutrients that most limits crop production on acid savanna soils (Rao et al., 1999). Studies on P cycling in long-term (16-year-old) introduced pastures in the 'Llanos' of Colombia indicate that legume-based pastures maintain higher organic and available P levels more consistently than grass alone or native pastures (Oberson et al., 1999). Greater turnover of roots and aboveground litter in legume-based pastures could provide steadier organic inputs and, therefore, higher P cycling and availability (Friesen et al., 1997;Rao, 1998;Oberson et al., 1999). Failure of P to enter organic P pools is thought to indicate a degrading system due to low level of P cycling (Friesen et al., 1997;Oberson et al., 2001).To overcome soil constraints and improve soil quality for agricultural productivity, there is potential for improved soil management through vertical tillage using a chisel plow (Amézquita, 1998a). In this study, we tested the hypothesis that vertical tillage combined with adequate fertilizer inputs to adapted crop and pasture germplasm will improve root growth which could avoid soil compaction and improve root turnover and accumulation of soil organic matter. We also hypothesized that this integration of soil tillage and soil fertility together with vigorous root systems of introduced pasture species could result in the build-up of an arable layer. The arable layer is defined as a surface layer (0-15, 0-25 or 0-30 cm depth depending on cropping system) with minimum soil physical, chemical, or biological constraints.We believe that the buildup of an arable layer is essential for low fertility acid soils to support sustainable agriculture (Amézquita, 1998b).The \"arable layer\" concept proposed here is based on combining: (1) adapted crop and forage germplasm; (2) vertical tillage to overcome soil physical constraints (high bulk density, surface sealing, low porosity and infiltration rates, poor root penetration, etc.); (3) use of chemical amendments (lime and fertilizers) to enhance soil fertility; and (4) use of agropastoral systems to increase rooting, to promote soil biological activity, and to avoid soil compaction after tillage.The main objective of this study was to evaluate the impact of different strategies of vertical tillage (1, 2, or 3 passes of chisel), crop rotations (rice-soybean), and crop-pasture rotations (rice-grass alone pasture; rice-grass/legume pasture) for 4 years on the buildup of an arable layer. Build-up of the arable layer was assessed in terms of improved soil physical characteristics (bulk density, penetration resistance), soil nutrient availability, soil P pools, plant growth, and nutrient acquisition.As part of a major effort to improve quality of native savanna soils for agricultural production in the Llanos of Colombia, a field experiment was established in May 1996 to determine the impact of vertical tillage, application of soil amendments and fertilizers, crop rotations and crop-pasture rotations on the buildup of an arable layer. The experiment tested two methods: (i) vertical tillage (using chisel) at different intensities (1, 2 and 3 passes) plus crop rotations to improve soil physical conditions in a crop rotation (rice-soybean) system; and (ii) vertical tillage plus use of adapted crop and forage germplasm associations (rice-grass/legumes) to improve soil through vigorous root growth, organic matter accumulation, maintenance of soil structure, and improved soil fertility.The experiment was carried out at Matazul farm (4º 9′ 4.9″ N, 72º 38′ 23″ W and 260 m.a.s.l.) located in the Eastern Plains (Llanos) near Puerto Lopez, Colombia. The area has two distinct climatic seasons, a wet season from the beginning of March to December and a dry season from December to the first week of March, and has an annual average temperature of 26.2 ºC. The area has a mean annual rainfall of 2719 mm, potential evapotranspiration of 1623 mm and average relative humidity of 81% (data from the nearby Santa Rosa weather station, located at the Piedmont of the Llanos of Colombia). Before treatment application, the area was under native savanna pasture, consisting for the most part of native savanna grasses. The land is generally flat (slope < 5%), the soil is deep, well structured and has a particle size distribution in the first 10 cm of about 34% clay, 28% silt and 38 % sand (loam texture). The soil has low fertility, particularly low available P because of the soil's high P-fixation capacity. It was classified as Isohyperthermic Kaolinitic Typic Haplustox in the USDA soil classification system (Soil Survey Staff, 1994).Use of acid-soil adapted upland rice and tropical forage germplasm in crop-pasture rotations has been demonstrated to be agronomically and economically viable on the infertile acid soils of the South American savannas (Vera et al., 1992;Rao et al., 1993;Thomas et al., 1995). Based on this strategy, the following treatments were designed to buildup an arable layer:• Upland rice (Oryza sativa L. cv. Savanna 6)-soybean (Glycine max cv. Soyica Altillanura 2) rotation with 1, 2, or 3 passes of chisel before rice planting in May of each year for 4 years. Soybean was planted in October and harvested in December of each year.• Rice-grass alone [Andropogon gayanus (Ag)] pasture, and rice-grass/legumes [Pueraria phaseoloides (Pp) + Desmodium ovalifolium (Do)] pasture with two passes of chisel before planting rice and pasture in May each year for 4 years. In both pasture systems, after harvest of rice in September, the pasture was allowed to grow until November. Pasture biomass was incorporated with two passes of disc harrow in November (end of rainy season) and also before planting rice and grass alone pasture association in May (early rainy season) each year.• Native savanna was used as a control to compare the impact of the above treatments with the natural (undisturbed) soil conditions.During the first two years, incidence of weeds in all introduced treatments was low and we did not apply any herbicides to control weed growth. During the next two years, however, we had to apply different herbicides (propanil, glyphosate, or 2,4-D) at recommended rates to control weeds in rice and soybean. The amount of aboveground biomass incorporated was between 3.5 to 4.5 Mg ha -1 of grass biomass for grass alone pasture and between 3.0 to 4.0 Mg ha -1 of grass biomass and 0.4 to 0.6 Mg ha -1 of legume biomass for grass/legumes pasture. Both grass alone and grass/legumes pastures were left ungrazed. We are aware of the fact that the agropastoral treatments in terms of incorporation of pasture biomass every year may neither be economical in short-term nor may reflect the current farmer practices. But we consider this as an important approach for improving soil conditions over a shorter time period than other options.Vertical tillage was applied in the following sequence: disc harrow, chisel(s), disc harrow to allow good seedbed preparation and sowing with a planting machine. Chisels were applied to a depth of 25 to 30 cm with a distance between chisels of 60 cm. The length of the chisel was 60 cm. Disc harrowing was applied to a depth of 7 to 10 cm with a distance between discs of 12 cm. The diameter of the disc was 60 cm.Dolomitic lime at a level of 1.5 Mg ha -1 to rice-soybean rotation and 0.5 Mg ha -1 to rice-pasture associations was applied via broadcast and incorporated with disc harrow one month before planting. Each year, at the time of planting, rice-soybean rotation and rice-pasture associations received (kg ha -1 ) 80 N (urea), 50 P (TSP), 100 K (KCl), 5 Zn (ZnSO 4 ). Soybean was planted each year after rice with residual soil fertility. Nitrogen and K were split-applied at 4 and 8 weeks for N and 0, 4 and 8 weeks for K after planting rice or rice-pasture associations.The experiment was laid down in a randomized complete block design with three replications in May, 1996. The individual plot size was 50 x 30 m. A composite soil sample consisting of 50 cores from each plot was collected in a grid pattern. These samples were air-dried, visible plant roots were removed, and soil gently crushed to pass a 2-mm sieve. The <2-mm fraction was used for subsequent chemical analysis. Measurements of soil physical characteristics (bulk density, penetration resistance) were carried out during the fourth year (June 1999) after establishment. Bulk density was determined using the core method and penetration resístanse was measured using a cone penetrometer (DIK-5521, Daiki Rika Kogyo Co., Ltd., Japan) (Amézquita, 1998b). Soil nutrient availability, shoot biomass production, root length, plant nutrient composition, and shoot nutrient uptake were determined for each treatment in September 1999. Soil and plant nutrient analyses and nutrient uptake were determined as described in Rao et al. (1992). Root length was measured using a root length scanner (Rao, 1998). Grain yield of upland rice and soybean were recorded after harvest each year (Sanz et al., 1999). The harvested area for grain yield determination was 2 qudrats of 5 x 5 m 2 in each plot.A shortened and modified sequential P fractionation as per the method of Tiessen and Moir (1993) was carried out on 0.5-g sieved (<2-mm) soil samples. In brief, a sequence of extractants with increasing strength was applied to subdivide the total soil P into inorganic (P i ) and organic (P o ) fractions (Phiri et al., 2001b). The following fractions were included: (1) Resin P i , anion exchange resin membranes (used in bicarbonate form) were used to extract freely exchangeable P i . The remaining P o in the extract of the resin extraction step was digested with potassium persulfate (K 2 S 2 O 8 ) (Oberson et al., 1999). (2) Sodium bicarbonate (0.5 M NaHCO 3 , pH = 8.5) was then used to remove labile P i and P o sorbed to the soil surface, plus a small amount of microbial P (Bowman and Cole, 1978). (3) Sodium hydroxide (0.1 M NaOH) was used next to remove P i , more strongly bound to Fe and Al compounds (Williams & Walker, 1969) and associated with humic compounds (Bowman & Cole, 1978). (4) The residue containing insoluble P i and more stable P o forms (residual P) was digested with perchloric acid (HClO 4 ). To determine total P in the NaHCO 3 and NaOH extracts, an aliquot of the extracts was digested with K 2 S 2 O 8 in H 2 SO 4 at >150 °C to oxidize organic matter (Bowman, 1989). Organic P was calculated as the difference between total P and P i in the NaHCO 3 and NaOH extracts, respectively. Inorganic P concentrations in all the digests and extracts were measured colorimetrically by the molybdate-ascorbic acid method (Murphy & Riley, 1962). All laboratory analyses were conducted in duplicate, and the results are expressed on an oven-dry basis.Analyses of variance were conducted (SAS/ STAT, 1990) to determine the significance of the effects of vertical tillage system and crop-pasture rotations on soil and plant parameters. Planned F ratio was calculated as TMS/EMS, where TMS is the treatment mean square and EMS is the error mean square (Mead et al., 1993). Where significant differences occurred, least-significant-difference (LSD) analysis was performed to permit separation of means. Unless otherwise stated, mention of statistical significance refers to α = 0.05.Bulk density values of different soil layers during the fourth year (June 1999) after establishment of the field experiment are shown in Table 1. Note the high bulk densities in the native savanna that served as a control treatment. Compared with native savanna, bulk density was reduced by the agropastoral and rice-soybean rotations. Consistent to the bulk density values, native savanna soil layers exhibited less total porosity (results not shown), which regulates the entry of water and the flux of air into the profile. Root growth is inhibited when bulk density exceeds 1.4-1.6 Mg m -3 and is suppressed at densities near 1.8 Mg m -3 (Heilman, 1981;Mitchell et al., 1982). Agropastoral (crop-pasture) treatments, in general, had 16% lower bulk density in the 0-10 cm soil layer and 13% lower in the 10-20 cm soil layer than those of the native savanna. In the subsoil layers, all treatments presented significantly lower values of bulk density than those of native savanna (Table 1). Previous research showed that legume-based pastures contribute to improved quantity and quality of soil organic matter with depth due to vigorous rooting ability of forage components (Fisher et al., 1994;Rao et al., 1994;Rao, 1998). Suitably low bulk densities are of great importance for soil management in this type of soil as they are indicative of factors that regulate root growth, infiltration, and water movement in the soil, which in turn affects nutrient availability in soil and nutrient acquisition by plants (Rao, 1998).`Results on penetration resistance at different soil layers are shown in Figure 1. In relation to native savanna, all the treatments decreased penetration resistance, particularly in topsoil layers (0-20 cm). These results suggest that it is possible to improve soil physical conditions to enhance water and nutrient availability, which favor rooting of the crop and forage components. The improved soil quality should allow these soils to support greater crop and pasture productivity (Amézquita, 1998b). Lack of additional effects of tillage on rice-soybean rotation compared with rice-pasture treatments (Table 1) indicates that either two passes of the chisel were sufficient in both systems or that deep rooting of introduced pasture species might have contributed biological tillage to improve soil quality. Both tillage and agropastoral treatments improved soil conditions, but whether one treatment is more beneficial than another over a longer period needs to be evaluated further.Soil chemical characteristics and root length distribution for different soil layers during the fourth year (September 1999) are shown in Table 2. As expected, compared to native savanna where nutrient availability was low and Al levels high, the different crop rotation and agropastoral treatments improved nutrient availability and reduced Al levels. The higher rate of dolomitic lime application (1.5 Mg ha -1 ) to rice-soybean rotation reduced the exchangeable Al level and increased the exchangeable Ca and Mg levels in comparison with the agropastoral treatments (0.5 Mg ha -1 ). Exchangeable Al levels decreased in the first two layers, but remained at similar values of native savanna below these depths. Differences in available P between rice-soybean rotations and agropastoral treatments were probably the result of differences in the rate of lime applied, which may have affected P sorption in soil. Other nutrients, such as K, Ca and Mg, accumulated in the topsoil. Nutrient values tended to be greater in the 0-5 cm layer as compared to subsoil layers. Available K was 2 to 4 times greater than that of native savanna (0.09 cmol c kg -1 ). Availability of Ca and Mg was 4 to 10 times higher than that of native savanna. These results suggest that application of lime and fertilizer could markedly improve soil fertility, particularly in topsoil. Chisel treatments were moderately effective to incorporate lime and P to deeper layers. Total C and total root length across the soil profile up to 40 cm soil depth were greater in agropastoral (rice-grass/legumes) treatment than those of rice with vertical tillage.To simplify interpretation of results, the P fractions were divided into three groups using a criterion similar to that given by Bowman and Cole (1978) and by Tiessen et al. (1984). The three groups were: (1) biologically available P, (2) moderately resistant P, and (3) sparingly available P. observed with the three chisel passes with crop rotation and the grass/legumes pasture treatments. The greater P o contribution to the NaOH fraction by agropastoral systems, the two-chisel-passes and the threechisel passes with crop rotation could be highly desirable because the NaOH-P o fraction is usually more stable than NaOHCO 3 -P o and may represent a relatively active pool of P in tropical soils under cultivation, especially those not receiving mineral P fertilizers (Tiessen et al., 1992). These results indicate that vertical tillage with three-chisel-passes with crop-rotation and two-chisel-passes with grass/legumes pasture treatments can markedly improve P availability through moderately resistant P pools. The sparingly available P includes the HCl-P (not done in this study) and the Hedley et al. ( 1982) residual-P. The sparingly available P is not available on a short time scale such as one or more crop cycles, but a small fraction of this pool may become available during long-term soil P transformations. In general, this fraction was slightly affected by tillage system in the top 0-5 cm soil layer (Figure 4A) and then remained fairly consistent through the rest of the soil profile. However, it represented about 49% and 73% of the total P in 0-20 and 20-40 cm soil layers, respectively. This fraction is mainly composed of the stable humus fraction and highly insoluble P i forms (Hedley et al., 1982) and was not affected by chisel, crop rotation and agropastoral treatments in the short-term. We also looked at the sum of the soil Po fraction (H 2 O-P o + NaHCO 3 -P o + NaOH-P o ) to detect any significant effects of treatments on P o that were not evident in the individual fractions. The sum of the soil P o fraction was, on average, 16% of the total P in the top 0-5 cm soil layer and decreased steadily to an average of 13.5% at the 20-40 cm soil layer (Figure 4B). The greatest amounts were obtained in the agropastoral systems, and the two-passes-of-chisel and three-passes-of-chisel treatments of crop rotation. The one-chisel pass treatment with crop rotation had the lowest effect on this fraction. Oxisols have high P-sorbing capacity resulting from their high Al and Fe content. Therefore, the increase of total P o resulting from treatment effects is desirable because the P maintained in organic pools may be better protected from loss through fixation than P flowing through inorganic pools in soil. Adsorption of P occurs mainly through processes in the soil, and as such minimizing P interaction with the soil is an important management tool for increasing P cycling. The trend in rice and soybean grain yields as a function of time is shown in Table 3. It was not possible to maintain yields of either crop in any of the treatments used. During the first year, yields of rice and soybean were relatively high, but they declined with time irrespective of the treatment, with the steepest rate of decline being recorded in the rice-pasture systems. The yield decline with rice may have been due to increase in weed biomass, which had a trend of 35, 320 and 704 kg ha -1 for the years 1996, 1997 and 1998, respectively, in rice crop across treatments. Soybean was relatively less affected by weeds and it failed to produce any grain during 1998 due to severe drought conditions. Shoot biomass of rice was less when associated with pasture components than under chisel treatments with crop rotation (Table 4). This could be mainly due to the competition of pasture components for nutrients, water and light. These results indicate that decrease in rice yields was much greater in agropastoral treatments than in rice-soybean rotation. On average, an increase in the number of chisel passes from 1 to 3 did not significantly affect rice biomass or grain yield production. Amézquita (1998a) reported that three passes could be excessive for these soils causing a collapse of soil volume. Shoot biomass of rice was greater with rice-soybean rotation than with rice/pasture treatments (Table 4). This was mainly due to the competition of pasture components for nutrients, particularly K and Ca which showed greater uptake in rice/pasture treatments than in rice-soybean rotation (Table 4). Previous research showed that pasture legumes could be of great importance in stimulating soil biological activity, nutrient cycling and addition of organic matter to the soil, which have beneficial effects on the production system (Rao et al., 1994;Thomas et al., 1995;Fisher et al., 1999;Sanz et al., 1999). Rao (1998) reported that the deep root systems of improved tropical forages are efficient in extracting nutrients from subsoil and recycling them throughout the plant and back to the soil through the death of plant tissue. Legumes also improve nutrient cycling and the nutritive value of forage. The agropastoral systems had greater root length compared to 1 pass of chisel treatment, particularly in the subsoil layers (Table 2). This is desirable as the turnover of roots through time contributes not only to nutrient cycling but also to soil improvement via positive changes in soil porosity and carbon sequestration in soil (Aerts et al., 1992;Rao et al., 1993;van de Geijn & van Veen, 1993;Veldkamp 1993;Cadisch et al., 1994;Fisher et al., 1994;Rao 1998).This study indicated that vertical tillage with 2 chisel passes for rice/soybean rotation or agropastoral treatments improved soil physical and chemical characteristics. However, these improved soil conditions did not translate into improved and sustained grain yields of either upland rice or soybean. This might have occurred because of crop management, particularly with the increase in incidence of weeds over time. Further research work is needed to develop appropriate crop management to benefit from the improved soil conditions. Buildup of an arable layer requires improvement of soil physical, chemical and biological conditions. Introduction of tropical pasture components with legumes into the production system could provide adequate soil physical conditions, to improve nutrient acquisition and recycling, and to facilitate accumulation of better quality and quantity of soil organic matter leading to the buildup of an arable layer. This study provides experimental evidence to promote the concept of building-up an arable layer in tropical Oxisols using vertical tillage and agropastoral treatments. But to improve and sustain crop production on infertile Oxisols of the tropics, there is a need to develop better crop management strategies to overcome weed problems. We suggest that the buildup of an arable layer is a prerequisite to move towards no-till or direct drilling systems to minimize environmental degradation in savanna soils of the Llanos of Colombia. The complexity, diversity and peculiarity of rural communities are increasingly being appreciated in research and development work. Social dynamics, like geographical, cultural, soils' physical and biological aspects of a given community pattern agricultural practices. This paper gives a systematic view and analysis of social networks in community institutions and demonstrates their importance and significance in the process of dissemination of agricultural knowledge. This paper is a result of a study within a BMZ-funded Project of the Tropical Soil Biology and Fertility (TSBF) Programme. The project sought to involve community institutions in the dissemination of agricultural technologies, and therefore studied mechanisms that could be effective in a socially heterogeneous farming community (Emuhaya, Vihiga District, Western Kenya). Some of the important matters brought to light in this study include:• There were strong social networks among local farmers. Important soil fertility knowledge given to the few farmers who had been involved closely in research and extension filtered to many other farmers through networks. • However, the content and scope of the knowledge was curbed in the process of dissemination.This resulted from the nature of the existing research links, which currently emphasise vertical rather than effective horizontal sharing of information.• The wide variety of social problems facing local farmers constrained their search for soil fertility knowledge. Farmers' efforts were diverted to short-term endeavours that ranked as more important. The understanding gained on networks in community institutions has provided a stage for wider and more meaningful involvement of farmers in research and extension. This paper (i) describes networks among farmers, (ii) demonstrates the importance of networks in community institutions and (iii) explains the function networks play in disseminating agricultural technologies among rural farmers.\"…[I]t is clear that agriculture today is often individualistic, with farming households increasingly going it alone. But in times of trouble, such as following a drought … and labour is critical to ensure a good harvest, networks are re-established and co-operative behaviour is very evident. Social networks based on lineage relations and friendship thus may not be critical to agricultural production in all years as they were in the past, but they are certainly of vital importance as a means of offsetting extreme hardship' (Scoones et al 1996:35). Social networks have an important role in the lives of rural communities. In the recent past, research has been done to understand networks and how they function. This knowledge needs to be synthesised, developed and analysed with the view of enhancing dissemination and adoption of agricultural technologies. Lack of understanding or inadequate appreciation of networks will impede dissemination of soil knowledge.Existing networks can be exploited through engaging community groups (IIRR, 1998). Because group members are themselves typically linked by a variety of network ties, individuals within the group are able to call on collective resources, such as reciprocal labour exchange (Sikana, 1995;Scoones et al 1996). Well-functioning groups develop a spirit of \"…unselfconscious showing, sharing, and checking… [such] Groups have an overlapping spread of knowledge which covers the wider field and crosschecks\" Chambers (1992:41). That implies that farmers of different sorts in such institutions share different types of knowledge and demonstrate or verify it in the process of interaction. By introducing technologies to strong groups comprised of well-networked individuals, researchers may have at their disposal a strong framework and impetus for the adoption of technologies (Sharp and Kone, 1992: 8).Even if strong groups permit the successful diffusion of technologies, researchers and extensionists must be wary of calling immediately for the strengthening (or establishment) of new community institutions. Farmers' institutions have developed and operate within a local context where they may be effective, but farmer groups on their own rarely have influence on higher-level decisions concerning policy. Similarly, because most of the rural population is resource poor, their institutions frequently have weak financial bases. This constant vulnerability does not allow such institutions to bargain with external donors or agencies on equal terms, and leaves them open to having priorities imposed upon them. Since many governmental and non-governmental organisations are eager set up their own farmers' organisations and to shape them in line with their ideas and ideologies, it is therefore crucial to establish practical guidelines through a truly inclusive and acceptable arrangement (Pertev and King, 2000). Empowerment should be about organisation, strengthening networking at grassroots structures. Allowing or enabling democratic control of members on these structures will help establish trust and effective vertical relationships.Although \"communities\" (or even farmer groups) may have boundaries and memberships that appear discrete and accessible to outsiders, social networks are not confined solely to those units. Rural people participate and invest in a diversity of social networks, whose value lies exactly in their wide thematic scope and geographic coverage (Adamo, 2001). It is ultimately going to be more sociologically and culturally sensitive, as well as more sustainable, to identify the range of local networks that could be used as potential entry points for different research activities (Sikana, 1995).However, it is unrealistic to expect that the interests and priorities of farmers' existing institutions will necessarily correspond with those of researchers. For example, Verma (2001) observed in Western Kenya that soil fertility concerns were not top priority for many farmers, even though land per capita is diminishing and the soil fertility in this region is under serious pressure. Greater worries about disease or marital security in fact constrained many households' efforts to conserve soil fertility. Truly participatory technology design will emphasise farmers choosing research activities relevant to their needs that also build on their knowledge of the farming system and their familiarity with local technologies (Haverkort, 1991).The process of study involved both individual actors and collectives in an effort to assess their links and roles, and to outline opportunities of better engaging community institutions for research and extension. To get a comprehensive view of the process of sharing of soil fertility knowledge, this study targeted community institutions, interviewing members of these institutions and focussing on key informants.Ten community institutions were purposively selected on the basis that (i) they were engaged in agricultural activities, (ii) held regular meetings, (iii) had been in existence for more than one year and (iv) engaged in information dissemination in several ways. Seventy-eight questionnaire respondents were randomly sampled from 334 members of these selected institutions. Seventy-eight (23%) was representative given the nature of the study, physical size and population density of the area. The purpose of the study was to maximise access to qualitative information from a group of respondents knitted in social networks. The researcher therefore deliberately selected a diversity of informants who were most likely to provide articulate answers, explanations, descriptions, proposals, and experiences concerning the complex issues of social networks. Eleven key informants were selected purposively. These informants included people with leadership roles and responsibilities (Maguru), those respected in the community, people who had worked in that community and had accumulated essential knowledge and experience in local agricultural aspects, with valuable opinion on community organisations. Some had participated in the project before and had valuable opinion on agricultural research. The researcher carried out several informal interviews, focus group discussion (FGD) and group interviews, engaged repeatedly in participatory learning and observed various phenomena to verify or identify data.The study (and therefore this paper) was largely based on qualitative data: on open conversations 1 , in-depth and group interviews, comparisons more than counting, informal FGD and brainstorming, stories, exchanges and consensus where necessary. Analysis was therefore largely on the spot, while the study occurred.3.1 -Study Area -Social. The study was conducted in Ebusiloli sub-location of Emuhaya Division in Vihiga District. The Luyia speaking people called Abanyore inhabit Emuhaya. They formed a close community in which there was much sharing of life between families and neighbours (Osogo, 1965). Today, a strong individualism is replacing this community consciousness. Since independence, their social system has noticeably transformed and the predominant religion is Christianity (Muruli et al, 1999).In 1995, Vihiga District had 948 registered women groups with membership of 28,440. There were 557 self-help groups (no membership given) and 168 youth groups with registered membership of 4,543 (Republic of Kenya, 1997). Information acquired from the divisional headquarters of Emuhaya during the study show that there were seven registered groups in Ebusiloli (Courtesy: Ministry of Social Services, Emuhaya Division). According to Muruli et al (1999), more than sixty three per cent of farmers in the area actively participate in different kinds of registered and unregistered community institutions.3.2 -Study Area -Physical. Emuhaya has loamy sands that support the growing of maize, sweet potatoes, coffee, beans, finger millet, sorghum, sugar cane, and horticultural crops (Republic of Kenya, 1997). However, these soils are quickly losing their fertility through leaching and over-cultivation due to limited land sizes per household coupled with low usage of suitable farming innovations. Vihiga district has an annual population growth rate of three per cent. Emuhaya's population density is about 1197 people km -2 (Republic of Kenya, 1997).Although networks are the implicit cornerstones of the farmer-to-farmer strategy for disseminating soil knowledge being preferred in many projects, many academic analyses of social networks create a romantic picture that may not be regarded as very useful by agricultural workers. Such texts may for example display a harmonious existence and functioning of networks in community institutions. Such information may not outline specific areas that can be exploited to enhance dissemination of agricultural knowledge. Of course, forming social networks is not an end in itself. The communication of information and reciprocity of networks are in reality a survival mechanism for many poor farmers. They provide the means to acquire material and non-material benefits.Results from the FGD, the study survey and in-depth interviews show that networks play a dominant role in agricultural production. The question remains, however, whether room exists for soil fertility knowledge in these social networks? Soil fertility knowledge may be spread or utilised depending on the type and strength of networks in operation. The following expressions by two farmers attest to this. Boaz:\" These verbatim accounts show that soil fertility knowledge is likely to spread faster among farmers bonded by strong networks, who will share valued information amongst themselves. However, as the second farmer implies, even information that is known to be potentially useful will not spread without it also being seen to be beneficial. In this case, because the soil fertility practices being researched were not perceived to offer immediate financial gain, farmers of that group were not keen to try them. This reluctance within the group of research farmers effectively blocked the further sharing of useful soil fertility practices, even though the research farmers might have been part of extensive and strong networks. This suggests that farmers would more likely engage in social talk about something immediately tangible, like a good yielding crop variety, than they would about something more abstract or complicated, such as a management practice that would directly support that crop by improving soil fertility. It is easier to talk about the benefits of a technology than it is to explain exactly how those benefits were obtained. Nevertheless, most of these new technologies rely on sound management. Because farmers will master this management at differing rates, many may not feel confident enough to discuss soil fertility management, especially the new practices that research is still yielding. Unfamiliar technologies (such as planting of Desmodium sp.) will therefore not enter easily into local discourse and practice. However, Crotalaria ochroleuca, another of the improved fallow species being researched and disseminated is well known by farmers because it is locally eaten as a vegetable. However, if it is known and liked then not enough quantities may be left in the farm to mature so as to contribute to soil fertility. Farmers may generally view it and discuss it as food, not a soil fertility management plant. Therefore knowledge about it may not necessarily be inclined toward improvement of soil fertility.One benefit of strong social networks is the provision of social fora to express or explore new ideas. While most farmers did not necessarily have access to such venues, two examples show how strong networks did allow valuable soil fertility information to be exchanged between members and sometimes used on collective plots. For instance, the 19 members of Emanyonyi Women Group spent between ten and sixteen hours (11-20%) of their working daytime in a week together. Discussion of soil fertility was part of their deliberations on the agricultural and forestry projects that generated their collective income. Each and every member was an official in some capacity. Because this group had several ventures, all the members had to actively contribute labour, cash and importantly information or logistics. Their stronger networks had not just inculcated discussion of soil fertility information; they attracted outside support (especially training and exchange visits). Similarly, Escrava Youth Group whose members were young (18-35), perceived soil fertility research as an opening to enhance returns from their farm projects. During a visit to their plot, the researcher noticed that they had embraced some of the biomass transfer practices that were under research. Most members of this group did not own individual plots, yet their willingness and ability to share soil fertility knowledge was astounding. Only two of the 17 members were participating in TSBF trials, but the level of awareness among the rest was significant due to their attitudes. Networks as strong as those found within these two groups act as invaluable 'talking spaces' and provide counsel to members. It is important for locals to be able to present and discuss new ideas especially in their own language and setting. This may present one way of understanding how farmers conceptualise and use new soil fertility ideas.Farmers with strong networks were able to exchange valuable knowledge on soil fertility (i.e.: biomass transfer and fallow cropping practices) and take certain collective investment risks. In such cases, the general 'farming' talk also led to tangible exchanges such as seed, agricultural labour, cash, manure, land use and other resources. Unlike average networks, strong networks allowed for a relaxed form of balanced reciprocity where one did not necessarily have to 'reply' immediately or directly. For example, one would volunteer one's farm for collective farming without expectation of direct payment from members. One was clearly aware that some members did not have sizeable farm to allow for collective activities.In spite of the decline in soil fertility and the ensuing low farm yields, farming remains a treasured activity, even if it is not one that receives adequate national support. Most people in the study area practised at least some form of farming regardless of their main occupation. One adaptive research farmer, Nashon, portrays this in the following explanation: \"I discuss many things with people. I sit in the [farmers research] group, and I know most of us want to spend a lot of energy on small businesses or to look for office work [i.e.: formal employment]. As you have known, our farms are very small and we are very interested in growing [i.e.: getting money] because we are very poor. This work you are doing will help us also, to train our people in agriculture, to teach us soil fertility practices so that we can increase our maize harvest. Let me tell you something, we feel very fastened to farming even though our maize [harvest] is pitiable. Yet one's own harvest is very precious. If your children want to roast [maize], they will not steal from your neighbour's farm [if your farm has any maize on it?]. So you see, soil fertility is important because if you fail to plant, or do not harvest anything, your child may become a thief and people will say you are lazy. So for me, I ask for soil fertility information from you the agriculture people [researchers and extension agents] and my friends [locals] to help us….\"As this commentary shows, farmers engage in agriculture not because it is inherently profitable but because it is an essential part of life. In this respect, it is wrong to conceive of peasant cultivation as a 'technical industry' per se, since it is also clearly a socially-based activity. Informal discourse within farmers' networks is commonly about what are accepted norms. Resources were 'injected into the soil' even when returns were small, in part because someone who 'failed' to demonstrate capacity as a 'farmer' would risk criticism as 'lazy', a 'thief', etc. Clearly the discussion of new soil fertility practices would fall within such local 'normal' discourse, but quantifying these discussions would be difficult.Nashon is however uses his local networks only cautiously, since he preferred not to rely on locals for important information. He stated that there are certain hindrances to the flow of useful knowledge within his experience of networks, especially related to the sharing of gossip (etsimbemba): \"You hear 'So and so said this or that': how do you rely on that? Avanti vayansa okhuchaya (others will despise you), instead of learning something from you. Valala vali nende omwoyo kwe imbotokha nende wivu (others have bad feelings and are jealous), others are false pretenders (vachuaji), they pretend they know it all and few are delinquent (avahalifu).\" Such feelings were particularly common among men. Many farmers cited such explanations as obstacles to the establishment of stronger networks with certain people in some community institutions. Farmers would rather keep away from those they regarded as pernicious, even if it meant foregoing access to useful information. An implication of this wariness is that in selecting resource people researchers need to be keen to avoid putting locals regarded as 'unpleasant' on the frontline. If they have bad reputation, the project will not have a good standing and locals may not feel enthusiastic about the research.On the other hand, some issues were perceived as so fundamental that farmers were encouraged to unite and create stronger networks. The main reasons behind strong networks of interaction in community institutions gathered in the sample survey have been given in Table 1 below. The most commonly mentioned motive for participation in community institutions was to improve members' livelihoods. Table 1 also reveals that at the grassroots, agriculture is an integral part of the broader system of economic and social contexts that shape involvement in social networking. . FGD, group discussion and informal interviews showed that earnings from other occupations such as formal employment and business are used to improve soil fertility. For instance, poor performance in business or a delay in salary payment affected purchase of fertiliser or seed. Therefore, networks formed on nonagricultural exchanges directly or indirectly support agricultural production. It therefore implies that narrow observation and dealing with local institutional networks as if they were purely 'agricultural' in orientation can be misleading.The history of an institution's development, and the nature of its members' networks significantly influenced the sharing of valuable knowledge, collective action and attitudes and behaviour. The main reason behind the existence of strong networks was the need to improve livelihood foundations. The strength of these networks depended on actors' trust in each other. If this trust was significant, they would invest their time and money together in group activities, one consequence of which would be the sharing of soil fertility knowledge.Nevertheless, the process of inception of the various institutions that existed was not unilinear. The strongest community institutions resulted from one or two individuals inviting their close friends who shared similar problems and aspirations. Leadership in such situation was achieved through mutual agreement, willingness and suitability. Duties would be delegated to any chosen member and everyone's input in drawing a constitution was valued. Constitutions were precise, mutual agreements, normally as written documents, but a few were verbal. They outlined the objectives, procedures and conduct of members in an institution.Researchers facilitated the creation of one farmers' research group, while other institutions were mobilised by local resource persons, church leaders, opinion leaders or even politicians. All the institutions that were selected for study had been in existence for over two years. The oldest was a labour group that had been in existence for over 25 years. Institutions that were formed and defined by narrow schemes such as to gain access to campaign money shortly before the 1997 presidential funds drives did not last long. They were mobilised by politicians and packed by people who did not have strong networks. Soon after inception, jostling for leadership and access to funds ruined them.There were several factors that were favourable for the existence of an institution. The overriding factors included the desire and dedication of actors in networks. Actors relied on existing traditions, and social and cultural attitudes conducive to co-operative participation and common social action. One discussant (Gerishon 2 ) reflects this in the following remark: \"Our people have always had reasons to co-operate. Since the time of my great-greatgrandparents, Abanyore have assisted each other in wars, famine, building huts, farming, hunting, raising children and so on. Many of those things are done individually now while others no longer matter. But, you never know. You may fall sick or even die tomorrow…. I have to maintain some relationship with some people, in the event that I need help they should be willing and available to assist me.\"The success of strong groups also tended to be self-reinforcing since, when members' financial stake in their group was significant they tended to participate actively in an effort to ensure that the group operated efficiently.The strength and nature of interactions within local institutions was significantly shaped by proximity. Most residents had been living in the study area among their lineage relatives for many generations. As a result, local level networks were considered strong. For example, members of Alexander's youth group live nearby and formed a very active institution. However, like other groups, the quality of networks was determined by friendship based on age and socio-economic factors.Table 2 shows that friendship was very important for determining group membership, especially within women and youth groups. Women groups, followed by youth groups, were identified as having the strongest network ties. These two drew membership from across the study area and beyond. Women were more available than men for meetings, able to work with young members, and had fewer leadership wrangles or funds mismanagement problems. Theoretically, networks were vast and primarily open. For instance it was not possible to map out all networks of a member of a community institution. But the nature of networks and goals of an individual defined the level of freedom to move in and/or out of an institution. For instance, a man of sixty-eight years was unlikely to share or discuss goals and aspirations with his twenty-year-old daughter in law. Other than age, this would also be culturally abhorrent. Many of the respondents belonged to more than one institution. However, this did not necessarily reward them with soil fertility knowledge. Members almost always affiliated closely with cliques of their closest friends. In fact, there was evidence of lack of practise and even awareness of notable activities done in other institutions in spite of the fact that some members belonged to both institutions. For instance, one institution had very strong networks that were evident from frequent weekly meetings, loyal contribution of subscriptions, high attendance in meetings, participation in collective activities and sharing of important farming and other knowledge. Members of this group had been trained in tree planting, improvement of soil fertility through improved fallow systems and biomass transfer, basketry and so on. Some of these skills and knowledge had not spread to neighbouring institutions to which some of the members belonged. It is possible that the content of the information that had percolated was very ordinary. It would take a longer period (more than one year) for a remarkable amount of soil knowledge to pass to other institutions and for them to use it.One explanation for the low exchange of ideas was that the new practices were not seen to promise good enough economic returns to compete with other group investment opportunities. Local farmers noted that agriculture is very difficult to capitalise. For example, farmers have trouble obtaining credit for new farm ventures because the profits to the lender are not assured. Furthermore, it was not feasible for most households or groups to engage only in agricultural activities, since land pressure is intense and it was difficult to acquire sizeable farms (over one acre). Even if farmers planted cash crops like kale or other vegetables, the amount they would fetch was little. Such collective proceeds would be meagre when subdivided among members of a given institution. Therefore, networks were not used exclusively for agricultural work. For example, if members set one day a week to work on their group plot, they would spend the rest of the week on other enterprises like selling maize or firewood beside the road. The desire for cash (specifically capital) was important in this respect. There was a resolve to engage in small businesses within homesteads or at local market centres and many groups intended to purchase plots, to construct shops, rental rooms, eating rooms, and so on. Such ventures were seen to be more profitable uses of local 'farm' plots than using that land for agriculture.Smaller institutions of about fifteen were more organised and committed to their course. It is however not possible to map out the boundaries of networks and thus determine an ideal size of farmers' institution. This study points out that it is preferable to work with institutions shaped out of a 'natural' process rather than try to create networks on the basis of research needs. If the goals of research cannot fit somewhere within the wider objectives and aspirations of local farmers, then there is arguably no business to be done with those farmers.In circumstances where local institutions are not already strong, creating or strengthening those existing networks can be worthwhile. It is not uncommon to find farmers who live nearby, share common agricultural problems and have strong networks, but who are yet to form an organised institution. In other words, the formation of a group does not happen spontaneously, it often takes the initiative of a local opinion leader or someone else to facilitate.According to Ritzer (1992), the strength of networks is directly dependent on the returns accruing from them. In reality, farmers do not mechanically act in social networks while making cost-benefit analyses. For instance, most farmers understand that seeking and maintaining links with researchers and extension workers can assist them to improve their soil management practices, among other benefits. Yet even where the researcher-farmer relationship is strong, it does not follow that information shared between them will spread to other farmers. The nature of farmer-to-farmer interactions is a function of local social relations, as explained by this fifty-six year old Liguru (village leader) Bernard: \"Many farmers in your projects do not want others to know what they are doing or how they are benefiting. They feel privileged and under no obligation to spread the knowledge they are given. They wish to stay on top by being advantaged in such ways…. Not all are like that, but it is hard to know. You [researchers] should do things openly. You should involve many people. Let us see, who has joined us here since you came? They [farmers] think it is a private deal between you and me. Those who come may not want soil fertility information but to know our affairs\".The acute under-utilisation of existing technologies is often construed as 'conservatism' or 'traditionalism' on the part of rural farmers. However, as Bernard's comments show, new ideas are not like seeds that can be sown on neutral ground. Instead, they are always being planted in environments where the \"safeguarding of vested interests and a way of life is very conscious\" ( Mbithi, 1974: 60).Networks are dynamic. Regardless of the rules and codes of conduct within community institutions, the networks between members are never constant. The more committed members are to their institution the stronger their networks. The more they exchange information and achieve their goals, the higher the chance that they will remain in those institutions for long.Congenial discourse within an institution creates an air conducive for association, but people will enter and leave institutions as they choose. Fortunes of members keep changing with new experiences, beliefs, opportunities, and even assumptions. In the face of such potential fluidity, an institution's organisation is important. Within institutions that had strong networks, members had unambiguous but to all intents and purposes transcending roles. In institutions where the role of each member was noteworthy, members had a feeling of value and merit. Such groups could endure without significant turnover of membership. However, most institutions did not function like this: rather, they had a small, core membership that provided continuity and direction. Most of these institutions were made of economically poor farmers. Such groups would not persist without the constant initiative of relatively stable members to invite, mobilise and encourage others to join or even remain in those institutions.Informal interviews, in-depth interviews and discussions showed that relatively richer farmers were seen as sources of casual employment, money handouts, food, tools, and so forth. Contribution of resources to institutions was supposed to be uniform for all members in given institutions. Nevertheless, relatively prosperous members of community institutions were expected to be 'monetary pillars' in times of difficulty. Chart 1 below shows the distribution of members of institutions in various social classes as perceived by farmers. These data were gathered in wealth ranking exercises that were carried out during the study. While poorer farmers acknowledged that there were also members who were knowledgeable in soil conservation, their value to the group as 'knowledge reservoirs' was rarely acknowledged or explicitly valued. The low flow of valuable knowledge from resource persons was also made notable as a result of their small number. Discussion also revealed that women were more likely than men to share resources in institutions. While soil fertility found its way (explicitly) on to the agenda of their discussions on relatively few occasions, women's strong networks were however built upon the understanding that they were more vulnerable to hunger and other local problems than men. It therefore became necessary for them to discuss crop performance. Furthermore, women's position in the homestead was precarious, as their stability depended considerably on their husbands' decisions. If they transferred savings to their homesteads, those savings would be counted as their husbands'. It was therefore rational to keep some of their savings with the group so as to safeguard it. Consequently, regardless of how women were perceived (wealthy or poor), their involvement in community institutions was beneficial. For instance, women perceived as relatively well off had children in schools and their parcels of farmland were relatively larger. If their husbands declined to hire labour, they were made to turn to their institutions for labour. In worse circumstances, poor women received comfort from these institutions. They would for example withdraw their investment in the event that they were divorced 3 to 'look for life' elsewhere. The proceeds of institutions were not necessarily immediate or for immediate use. As Kanogo andMaxon (1992), (in Nangendo, 1994:353) say, members of these groups:\"pooled labour resources and shared the proceeds of that labour, and this produced a spirit of sharing and unity. People joined these groups even if they did not immediately need the services provided.\" Hartwig (2000:34) observed in a similar vein that:\"The groups are open to precisely those women who … progressing from dealing with specific problems in the women's work and life, provides the stimulus to exchange information and develop their education.\"To a casual observer, the networks in local institutions are besieged by competition just as much as cooperation. It was however found that competition in these institutions acts as a reminder that farmers do not always network because they are homogenous, but rather because they want to fight poverty. This finding is reflected in this revelation of a sixty-year-old key informant (Repha):\" Her emphasis on tangible results reinforces that that research must be seen (and known) to benefit especially poor farmers. If the agricultural technologies that research is generating can be shown to be useful and sustainable, then researchers may rely on networks to reach the wider community. During the study period, it was found that farmers were willing to participate in collective research activities if research would directly benefit them especially in the short term. Some of the cover crops (especially Desmodium, planted with Napier grass etc.) were appreciated. Some farmers liked this particular leguminous cover crop because it would increase the harvest of Napier grass, which is an important cash crop to many institutions that were studied. However farmers were disheartened due to unavailability of seed. Moreover, if these legumes could be consumed (like Crotalaria) it would be pointless to vigorously disseminate knowledge on their usefulness. Such knowledge would spread quickly through social networks and farmers would first adopt them as food crops regardless of what scientists told them. The importance of informal ways of information dissemination and sharing are difficult to overstate. Table 4 below shows results from a survey carried out by TSBF in 1998, which included the study area. Informal networks were mentioned more than twice as often as being a source of agricultural information than the government extension service (the next most cited source).Despite its prevalence, the success of research and dissemination using informal channels is not straightforward. For instance, knowledge transmission is not based on simple communication channels, conduits or linkages, it involves human agency and occurs within socially and politically constituted networks of different actors, organisations and institutions. Thus, communication occurs through the discontinuous, diffuse, value-bound interactions of different actors and networks (IIRR, 1998;Scoones and Thompson, 1993). At the same time, researchers should not expect that the content of 'their' information would pass through networks unaltered. For information to be locally relevant, it must be continuously tested and validated. Researchers relying on local networks for knowledge diffusion must also embrace the idea that the learning process involved will be 'two-way', with lessons to be learned by both farmers and researchers.Because networks depend on the decisions of individual actors, distribution and content of knowledge cannot be precisely monitored. Network channels filter information differently to different actors. Effective dissemination of information will therefore have to rely on involving collectives. Relying on stronger networks in community institutions represents best the promise of wider reach to poor farmers. Giving information to as many farmers as possible at once improves the chances of information spreading widely through existing social networks (Grigg, 1995).Farmer-to-farmer extension that involves community institutions provides a variety of both formal and informal settings for farmers to learn and share knowledge through participation. Researchers have been working with community institutions in the study area in a variety of activities. The most important venues cited by respondents included: demonstrations mentioned forty-three times (55.1%), field days mentioned thirty-seven times (47.4%), farm visits mentioned thirty-six times (46.1%) and training workshops mentioned twenty-two times (28.2%). These activities presented convenient avenues for researchers to interact with farmers while farmers also got to learn from each other. Although farmers lived close by to one another, in normal, daily interactions they reported rarely finding such valuable occasions or venues to learn new ideas (or even unlearn poor practices). Although researchers considered such 'on-farm' work to be less formal than their other types of interactions, farmers strongly associated such fora with technical knowledge. The talk by both farmers and technical staff was not general; it was direct and vital in enabling farmers to separate facts and reality from rumours. Farmers got to learn with confidence -information validated as 'science'-and to assess things through their own eyes. Such fora also encouraged change, unlike informal interaction where conformity with the status quo was often the norm.Despite the high status afforded to the formal activities, all seventy-eight farmers reported sharing the bulk of their information through informal activities. Such activities included group meetings, interaction at local market centres, farmer-to-farmer visits, singing and so on. For instance, when outside 'visitors' came to the village, women would sing songs containing soil fertility messages. While this certainly served to entertain, it was also a very powerful means of disseminating soil fertility knowledge. One may conclude that those songs bear witness that women had taken into account the soil fertility practices that research was yielding. The songs also further strengthen networks among members and encouraged visitation.During formal events such as demonstrations, consultations between farmers and specialists in a less formal, impromptu atmosphere before dispersing helps answer questions that could not have been addressed during the formal programme of the occasion. This presents an ideal arena for farmers, especially those who would not normally speak in public gatherings (such as poorer women and children) to appropriately learn.Researchers need to take explicit steps have to respect women by listening to their side. Otherwise women's often-placid approach may sometimes be mistaken for ignorance or disinterest. Because resource poor farmers (especially women) are besieged by numerous problems, scientists and extension agents should expect them to bring up topics that are not directly connected with soil fertility. Being the topic of the day, soil fertility may only act as an 'entry point' for dealing with broader development issues. In the midst of what appeared to be a soil-focused discussion, a participant may, for example, ask for a water or dairy project. Such concerns are common and, while seemingly 'off-topic', the outsiders' remarks on them will be keenly noted. Farmers may afterward reflect on what they did not hear from the researcher, and it is important to bear in mind that such dialogue is not uniquely a 'research' endeavour, but part of a longer-term social relationship. It is neither realistic, nor practical to assume that a topic like soil fertility will feature in every social encounter, as demonstrated by this comment by a farmer (Jairo): \"I cannot introduce a technical subject like use of fertiliser on the market or while taking busaa [a local beer] with my friends. They are supposed to be here listening [referring to a field day]. Furthermore they are not children to be taught on the proper practice anywhere…. However, you [researchers] should keep up, I have observed an increase in the number of attendance by farmers. Also, your activities have encouraged more [community] discussion on agriculture and trees [agroforestry].\"While it is entirely legitimate to focus on soil fertility research, crusading to keep farmers consistent may push them away or simply turn them into 'followers'. For instance, at the outset of this study the researcher explained to farmers that he had gone to the community 'to learn'. However, from rapport development up until the end of the work, farmers expected him to be an instructor. During formal interviews, respondents would keep 'other' issues to themselves. But when interacting informally, they would for example say, \"Ooh, it is good you are with us. We look forward to your help…\" or, \"Take these vegetables, you are a good young person, you can make a wonderful son-in-law….\" Farmers who were able to express their various 'off-topic' feelings or remarks and questions were then more willing than before to discuss soil fertility issues. The researcher had penetrated their networks somewhat by allowing the informal 'talk' to be his 'entry point'.There is also a need to understand that different farmers learn well in different settings. Understanding the learning styles of the farmers who researchers and development agents deal with can assist them to establish suitable platforms and processes. Chart 1 (above) showed that more than three quarters of local farmers were resource poor. The interview process found that most of them preferred a balance between the informal forms of knowledge dissemination that were typical within their groups and more formal means. The following is an example of informal aspects as suggested by a farmer during an FGD: \"…. Those [farmers] who are using your ideas [soil fertility practices] can be selected as examples to show the way. Also, all participating farmers should be awarded certificates. Certificates will remind us about your work and encourage us to talk about it openly.\" Such encouragements, while perhaps seeming trivial to an outsider, would actually be like 'adding value' to any information that is provided. Locally, education is highly regarded and any proof that one is knowledgeable may enhance one's confidence. This may not directly contribute to a change in farming practices, but if it can generate enthusiasm in research activities so can it improve communication in social networks.While every farmer involved in this study was involved in social networks, the use of these networks for farmer-to-farmer extension is as complicated as the networks themselves. To disseminate agricultural knowledge through channels that have evolved for a multitude of social, cultural, and economic reasons, one needs to understand the local institutions, agricultural practices and preferences of local farmers. Therefore, researchers and extension workers need to understand the social interactions within local institutions and how these institutions can better serve memberships from varying socio-economic backgrounds.There is also a need to involve farmers in community institutions directly in the dissemination of knowledge. Transferring more of the research and dissemination process off-station and into farmers' hands should not just be viewed as a cost-saving measure, but one of legitimate empowerment. Currently, local innovation is being hampered by the farmers' (often-justified) anticipation that the researched soil fertility technologies generate poor returns. If farmers can monitor and evaluate their activities, the costs and benefits of innovations can be better understood and a subsequent generation of more relevant and effective technologies will result. We can also expect to see greater farmer commitment and thus the greater involvement of their social networks. Such an evolution may involve unlearning old practices on the part of both development agents and farmers. It was observed in this study, as in previous ones, that greater facilitation and involvement of farmers as full partners in research and dissemination would enhance innovation of indigenous technologies. Researchers and extension agents also need to acknowledge that the sharing of information through networks is by definition a 'twoway' learning process, which ultimately generates important understanding of local realities. Questions that need to be clearly understood include: how and when do farmers innovate, who amongst them is foremost in innovating and how do they trust technologies from within and outside and share them amongst themselves?Decision Support Systems for Integrated Soil Fertility Management J.J. Ramisch and M. Misiko TSBF-CIAT, PO Box 30677, Nairobi, Kenya Rationale: Adaptation by farmers of research-designed technologies is crucial for increasing the relevance and therefore adoption of technologies. Adaptive research has to be linked to increasing the capacity of service providers and farmers to disseminate this new information and to ensure effective information flow between research and extension.Since May of 2002, four interactive participatory events have occurred at a TSBF-farmer demonstration plot in Emuhaya, W. Kenya. Efforts have been made to describe and explain to a wider audience (local farmers) TSBF's process of soil fertility research in W. Kenya. Key among the explanations were that TSBF in conjunction with local farmers and other research institutions have identified soil infertility and related problems as major and are researching on alternative or/and potential solutions.Some of the technologies that have been researched are being illustrated on the demonstration plot in Emuhaya. These activities include i) improved fallows ii) efficient recycling of residues iii) use of inorganic and organic fertilisers iv) traditional practices like natural fallows and use of indigenous plants among other technologies. On July 31, 2002 farmers and researchers held an evaluative field day and discussions at the demonstration site. Farmers expressed the main areas of strength and pointed out improvements that were needed. On August 16, 2002, farmers and TSBF staff harvested maize on the demonstration plot in Emuhaya. On August 22, 2002 a community discussion was held involving different types of farmers and TSBF staff to assess achievements, limitations and lessons achieved from the activity. On the former day, a pre-harvest evaluation was done, and also ranking of the different plots under different treatments. On the later day, in-depth deliberations were held about the plot and way forward. This was a furtherance of preliminary discussions that had been held during different stages of the demonstration.The technologies that were demonstrated on the plot include i) efficient recycling of crop residues ii) use of inorganic and organic fertilisers and their iii) different combinations iv) biomass transfer and use of legume trees. Also tackled, and not demonstrated, include use of indigenous plants as manures.Findings: There was a step-wise pre-harvest review of plots under different treatments by farmers and TSBF staff. Many of the attendants had visited the plot before and many had participated in various or different activities at the site and were already acquainted with the plots. A summary of all activities that had been done on and at the demonstration plot was given and the plots were briefly described; the different treatments included: Low quality (maize stover)High quality + polyphenols (Calliandra calothyrsus)Control plot (no organic)No N or P + Urea only + Urea, +TSP A rapid pre-harvest assessment of plots was held; first, amongst the first five (with organic inputs only); second, between plots with organic inputs alone and those with different inorganic fertilisers added; and three, amongst those with inorganic fertilisers. This was done to illustrate nutrient contributions of selected inputs.All maize plants on every plot were counted. After getting the sum of maize on every subdivision, farmers systematically harvested the crop, starting with the control and weighing both stover and maize. Various observations were made, and notes on the following taken by farmers: Differences in dryness, weight, appearance; Effect of striga, other weeds and pests.Most maize had been twisted, lying on the ground due to a past storm. Low harvest was also blamed on late planting and a long dry spell in June. Farmers said that under normal local circumstances one would likely get some harvest, however small, and not to completely miss out even when no input is applied as happened under the control plot.Variability within plots: The soil type on the plot is called ingusi. Ingusi is yellowish brown to dark brown, clayey soils. It occurs commonly in Emuhaya according to the soil study that was done in February 2002. Striga was very prevalent on plots with high fertility, especially where TSP was applied. This contributed to low harvests on those plots. It was clarified that inorganic fertilisers do not 'bring' or increase striga weed as expressed by few farmers during the discussion. Rather, fertility conditions needed by striga were created on those spots where TSP was used.Ranking of treatments: Plots were ranked on the basis of: Green leaves -before drying; Thick and long leaves; Height of stalks relative to seed type (hybrid etc.); Bigness of maize, and cobs; Germination rate; Rate of growth, especially after germination, is determinant; Number of cobs on every plant; Number of lines of maize on every cob, especially important in selection of planting seed [12-14 lines (hybrid 513), 16 lines or even more (pioneer usually has one cob with large seeds). Number of lines is relative to type of seed. Also size of every seed matters. Small seeds are good for roasting]; Weight of maize, through estimation by hand and observation.Farmers used these criteria to rank the various plots. The table below shows results of the ranking. The Table also displays results of previous ranking that had been done and documented two weeks before harvest. 1 shows a shift in ranks, between FYM and Tithonia. This change came as a result of weighing; maize on Tithonia plot was heavier than that on FYM plot. During preliminary ranking, FYM was seen to have had a more positive role in the context of the demonstration trial. Then, the overall aspect was the size and not weight of maize cobs. On the basis of this, FYM had performed better because it would result in higher yield.The order of ranks was respectively similar for plots with organic resources listed above with TSP alone, and TSP and DAP added. It was easier for farmers to tell differences between segments with organic resources only, unlike comparison between those with inorganic fertilisers. Farmers therefore deduced that certain mineral components (i.e.: P) can only be adequately sourced from inorganic fertilisers. P had been unknown to majority of farmers before prior demonstration events were held.• Good harvest (quality and quantity) depends on:o Availability of rain, type of rain o Timing of planting (recommend that TSBF plant at same time as the majority of farmers to enable them to better compare with and borrow ideas from trial site). o Type of soil (recommend that TSBF locate trial sites on each of the major local soil types). • Appropriate use of high quality resources like Tithonia was seen to have a bigger potential locally. However, labour to harvest Tithonia was highlighted as a constraint.• Use of stover as fertiliser was seen as less promising option. Stover is used as fodder and as fuel material, and because it is usually available in small quantities, abandoning it in the field is not very feasible. Other ways to use it more productively should be devised, or to feed it to livestock and to use the resultant dung as FYM.• The site allowed farmers to better learn how to tell whether plant material is good manure e.g. softness, quick to rot, easy to tear, bitter taste. Masatsi, mirembe che sisungu etc.• There is lack of cash to use available technologies, especially because of lack of P in most local soils and the need to buy P-containing fertilisers. • Farmers' comments that the demonstration plots should be 'large' reflect concerns that local farms display considerable variability in soil quality even over small distances and that it is difficult to extrapolate performance from 3 x 3 m plots. This variability results from concentration of resources on certain sections of the plot -driven by labour shortage, on the basis of what section is more promising. These sites tend to be where there is more fertility and where farmers tend to plant first. There are certain sections (especially near the house) where organic materials are dumped regularly.• There was much interest in doing more demonstrations; with the current awareness, more farmers are likely to attend and learn from the process.• Disease and funerals affected attendance in field days at the plotThe depletion of much of sub-Saharan Africa's soils through continuous cropping and decreasing nutrient inputs has been widely reported over the last 10 years. All land on the African sub-continent that is classified as very suitable for cultivation was already under cultivation 15 years ago (FAO, 1986). Farmers are increasingly intensifying their agricultural production activities, for example, through the more efficient utilization of animal manures, crop residues and forages and other organic resources. At the same time, use of inorganic fertilizers is decreasing such that organic resources are often the only source of nutrient inputs for farmers (Giller et al., 1997). Resource-poor farmers face difficult decisions over the use of scarce nutrient sources in their production systems. In addition, land constraints force them to trade-off land use in terms of food crop production, fallowing and animal feed supply, amongst others.Much research has highlighted the benefits of fast-growing legume cover crops (LLCs) to supply nitrogen fixed biologically from the atmosphere to a following non-legume crop in a rotation (e.g. Fujita et al., 1992;Gachene et al., 2000;Giller et al., 1997;Palm et al. 1997). Short growth duration legumes have been used to replenish soil fertility in many parts of Africa (Gutteridge, 1992;Dreschel et al., 1996;Luna-Orea et al., 1996;Jama and Nair, 1996;Gachene and Palm, 1999;Rao and Mathuva, 2000;Kayuki and Wortmann, 2001). LCCs refer to the production and incorporation into the soil of leguminous crops that have been grown to enhance the yield of following crops (Lathwell, 1990). Maximum benefit of this approach is seen where the biomass is incorporated into the soil early giving rapid release of nitrogen but farmers often have other priorities in their production system. This can be in terms of a demand for livestock feed, for production of a durable mulch or to control weeds like couch grass. This implies that the farmer may not always manage for the optimal but balance the needs of their farming enterprise. An understanding of how much of the biomass should be incorporated to increase maize yields, where and how this biomass can be produced and what are the alternative uses of the biomass are critical to targeting these legume cover crop technologies.In Uganda smallholders dominate the agricultural sector with over 90% of crops being produced on household farms averaging less than 2 ha (Appleton, 1998). More than 60% of the land is under cultivation with declining fallow length and increasing periods of continuous cultivation (HASP, 2000). This has greatly reduced crop yields, in addition to increasing pest and weed problems (NARO, 2001). The fallow period has reduced from about 10-12 years of secondary forest fallow in the early 1980s to 3-5 years at present, with the number of crop cycles between a 10-12 year fallow increasing from 2-3 to 4-7 during the same period (Boonman, 1999). Due to these land constraints, the utilization of biomass transfer and legume cover crops was investigated as options for soil fertility management and also in terms of the niches on-farm where they can be grown to fit within the existing production system. Critical to targeting these legume cover crop technologies is an understanding of how much of the biomass should be incorporated to increase maize yields, where and how on the farm this biomass can be produced, what are the alternative uses of the biomass and what are the opportunities and constraints to technology adoption identified by farmers.The objectives of the study were, (i) to evaluate the effects of Mucuna pruriens and Canavalia ensiformis on maize yields, and nutrient balances, (ii) to determine the effects of different management options for Mucuna and Canavalia (full vs. partial incorporation of above-ground biomass into the soil) in farmer-managed trials in maize-based system of eastern Uganda and (iii) use farmer evaluations of the technologies to assess their potential adoption and to identify areas of adaptive research.Farmer managed on-farm experiments were conducted on five farmers' fields in each of two sub-counties (Kisoko and Osukuru) in Tororo District, Eastern Uganda. Both sub-counties are 1000-1200 m.a.s.l., receive bimodal rainfall of 1000-1200 mm per year and maize, groundnuts and cassava are the main crops. The two sites differ in their geographical position with Kisoko being about 20 km North of Osukuru with soil fertility and rainfall, particularly the chance of extended dry spells increasing as you go North. The soils are generally sandy clay loams (Kandiusalf) and have good pH and are generally low in organic C, N and P. The sites significantly differ in their clay content (16.3 vs. 31.3) and total soil N (0.05 vs. 0.11) for Kisoko and Osukuru respectively with all other parameters being non-significant (Table 1).Following the harvesting of an unfertilised maize crop, Mucuna (75 cm by 60 cm) and Canavalia (75 cm by 30 cm) were planted as sole crop fallows for one season. No amendments were added to the soil at the time of legume planting so that the legume cover crops would be produced under farmers' conditions. At the beginning of the next season the legume cover crops LCC were cut, allowed to wilt for five days and incorporated into the soil at either 50% or 100% of the in-situ produced biomass. The treatments were as follows: 1) absolute control, 2) fertilized control, with P and K application, 3) 100% of above-ground Mucuna biomass incorporated, 4) 50% of above-ground Mucuna biomass incorporated, 5) 100% of above-ground Canavalia biomass incorporated and 6) 50% of above-ground Canavalia biomass incorporated. A basal application of TSP (80 kg P ha -1 ) and Muriate of potash (60 kg K ha -1 ) was broadcast and incorporated to a depth of 15 cm in all plots except treatment one. Farmers managed the experiments and conducted weeding and other management practices as they would for their own farm.A RCBD was used with five replicates, with each of the five farms acting as a replicate. Maize (hybrid Longe1) was sown at a spacing of 0.75 m by 0.25 m, with two seeds per hole and was thinned to one seed per hole after two weeks (53,200 plants ha -1 ). Plots were kept weeded during the farmers normal weeding operations. At physiological maturity net plots (3.75 m by 4 m) were harvested and the weight of maize stover, grain and cobs recorded. Sub-samples were collected, chopped and dried at 70°C for 48 hours. After harvest, all plots were cleared of weeds and crop residue and hand ploughed. In the following two seasons maize (hybrid Longe1) was sown to investigate the residual benefits of the LCCs.Soil samples (0-15cm) were collected at the beginning of the experiment and bulked by sub-county for analysis. Samples were analysed for pH (water), Total N (Kjeldahl digestion), Total C (Walkley-Black), extractable P (Olsen and Sommers, 1982), macronutrients (extracted in NH 4 Oac; atomic absorption spectrophotometer) and texture. LCC plant samples were analysed for total N (Kjeldahl) and total P and K (Kjeldahl; atomic absorption spectrophotometer; Parkinson and Allen, 1975).Farmer participatory evaluations were conducted using open questions and probing questions. Qualitative data was also collected on farmer criteria used in selection of LCCs technologies and on innovations made by farmers.Data were analysed by the SAS General Linear Models procedure (SAS Institute Inc., 1988). ANOVA was used for mean separation and significance is reported at the P<0.05 level.Comparative analysis of the macronutrient content of the LCC's shown there was only a significant difference in the plant species for their Calcium contents (Table 2). There were no significant differences in location or in the species by location interaction. Similarly, there were no significant species or location differences in the biomass production of the two LCCs, nor in the amount of N applied in the experimental design. The amount of biomass produced was not significantly different but for both locations Mucuna and Canavalia exceeded 6 t DM ha -1 and 7 t DM ha -1 in Kisoko and Osukulu, respectively.There was no significant difference between the sites except for stover production in the first season (Table 3). Therefore data was combined for further statistical analysis. No significant increases between the control and the positive control (with P and K addition) were observed indicating that the site was N limiting and not P or K deficient.In the first season, at both sites, all treatments (except 50% incorporation of Canavalia biomass in Osukulu) gave significantly higher grain and stover yields compared to the control (Table 3). In all cases where biomass production was significantly higher than the control, the yield increase was more than 100% higher than the control treatment. Incorporation of 100% rather than 50% of the biomass produced in the plot did not significantly increase maize grain yields compared to the control (Table 3). In the second residual season no significant differences were observed for maize grain and stover production between treatments. Combined yields over the two seasons showed significant increases in maize grain yield for all treatments except for maize stover production for maize grain with the 50% Canavalia incorporated treatment. The total dry matter yield of grain and stover over the two seasons was highly significant (P<0.001) for all treatments (Table 3).The farmers normal practice of growing maize without additions of organic or inorganic fertilizers not surprisingly resulted in negative nutrient balances for N, P and K (Fig. 1). In the first season after incorporation of the LCCs, addition of both 100% and 50% of the aboveground biomass reversed this negative nutrient balance for N only. Additions of 100% of the aboveground biomass of either Mucuna or Canavalia were needed for a positive nutrient balance for K, whereas none of the treatments produced a positive balance for P (Fig. 1). Nutrient balances were not calculated for the residual seasons as there were no significant differences in maize grain and stover yields.Farmers assessment of the LCCs species revealed many positive and negative aspects for each species (Table 4). Many positive criteria were mentioned by farmers that were expected for these species, for example, improved soil fertility, provides livestock fodder. More interesting from the research prospective were the negative aspects. For example, Mucuna and Canavalia were notably disliked because their seeds are not edible yet they looked very attractive to eat, and are produced in large numbers, even in dry seasons. Mucuna was further disliked for its unsuitability for intercropping and because it can harbour snakes if planted near the homesteads.The lack of significant differences in biomass yield of the mucuna and canavalia (Table 2) at the sites in spite of Kisoko being more deficient in N (Table 1) would suggest that similar BNF among the two species at both sites. Biomass dry matter productivity averaging 6.8 t DM ha -1 for Mucuna and 7.0 t DM ha -1 for Canavalia in six months compares with other reported data. For Mucuna pruriens other authors have reported varying sole crop one-season biomass production figures. Mucuna produced 1.3-3.50 t DM ha -1 and in some cases up to 9 0 t DM ha -1 in one season in Rwanda (Drechsel, et al., 1996); an average of 5.7 t DM ha -1 across five sites in Malawi (Kumwenda and Gilbert, 2000) and an average of 4-7 t DM ha -1 from sites across Kenya (Dyck, 1997).Comparative data for Canavalia ensiformis has been much harder to find. Gachene et al. (2000) report production figures of 3-6 t DM ha -1 in six months. These different production figures are due to differences and variations in soils, rainfall, seasons and management of the legumes for the different citations.All significant treatments increased maize grain yields by between 106% and 118% in the first season, these yield increases more than compensate the farmer for the loss of one seasons production of maize whilst the legume cover crop is being grown. Kumwenda and Gilbert (2000) reported maize grain yield increases averaging 180% following Mucuna incorporation in five sites across Malawi. Whilst other studies have not reported such yield increases of above 100% and therefore did not find that the extra maize yield compensated for the land being out of production for one season (Drechsel, et al., 1996). Also, work in Uganda work with different legumes found that maize yield increases varied by season and legume species but on average did not consistently return yield increases of above 100% (Fishler, 1997;Tumuhairwe, 2001).Although the grain and stover results were not significant in the second season it has to be remembered that farmers under their own conditions, with individual farmers as replicates, conducted this work. Therefore, the level of error would be expected to be higher than a replicated on-station or a replicated on-farm experiment. The second season results were significant (P<0.10) for maize grain and stover yield, giving the farmer a two-season benefit.Where the yield increases do not compensate for the loss of maize production during the season of LCC production there is unlikely to be any adoption in areas of high population density where there is a high demand every season for cropping land. The advantages of LCC can best be utilized where land is out of production due to low fertility or high pest/disease pressures or where it would be left in a natural fallow system. In addition, the significant increases in associated maize stover provide increased options for the farmers. The extra stover can be used in livestock feed or bedding, soil erosion control, compost making or mulching the banana crop.Another very important conclusion of this work is that incorporation of 50% or 100% of the insitu produced biomass produces a maize grain and stover yield that is not-significantly different from each other. This again then provides the farmers with increased options for their resource management. For example, this would allow the farmer to produce the biomass in one place and to apply the biomass over twice the area for maize production. Alternatively they might want to use 50% for incorporation and the remaining 50% for livestock feed, sale to other farmers or to produce hay for dry season feed. Increasing the resource management options and therefore the production options of the farming enterprise is critical where land sizes and fallow areas are small and little area is available for non-food crop production and where cash is not-readily available to buy inputs for crop and livestock production.The N fixed by LCCs during the fallow period may not a net addition to the system if increases in the following crop yields removes more N than is added by the legume. The large applications of N in the LCC biomass (Table 2) will be exposed to leaching as decomposition occurs, especially during the tropical storms that characterise the beginning of the rainy season. Much work has been conducted on decomposition and nutrient release rates for legumes, with different rates being reported. For example, more than 50% of N, P, K and Mg from Desmodium and Pueraria being released in four weeks (Luna-Orea et al., 1996); 60% of N from Leucaena and Senna in the first four weeks (Jama and Nair, 1996). Drechsel, et al. (1996) concluded that as sowing starts immediately after the first lasting rainfall, it is impossible to optimize the time of green manure incorporation before sowing. This may be true but there are management options available to reduce leaching of N and increase synchrony of release with demand. For example, incorporation of sorghum straw and green manures significantly delayed and reduced leaching of nitrate, by around 30%, of the mobilized legume nitrogen (Hagedorn, 1995in Dreschel et al. 1996). Similarly, other nutrient balance studies in Uganda have reported negative balances for a range of cropping systems (e.g. Bekunda and Woomer, 1996;Wortmann and Kaizzi, 1998).There was no doubt among the farmers that the LCCs technologies work and were better than the traditional practice as far as improving soil fertility was concerned. In terms of costs, it was reported that the use of LCCs and biomass transfer species offered a low input technology to the farmers, as most of them could not afford use of inorganic fertilizers especially on the low value crops like maize. Farmers evaluation however raised many concerns over the adoptalibility of the technology. Farmers observed that the use of LCCs required a substantial amount of land for production and for sole crop production this is left under fallow with no food crop being produced, high labour for clearing and ploughing in the vegetation, and patience in attaining the results (Table 4). In addition, the single purpose use of the LCC was mentioned as a negative aspect in terms of the adoption of the technology.In the on-farm experiments reported here in two areas of Uganda, the use of Mucuna pruriens and Canavalia ensiformis significantly increased the following maize yields in the first season (P<0.05) and in the residual season (P<0.10). Farmer evaluations of the technology highlighted some negative aspects of this technology, for example, it needs increased management by the farmer as well as increasing the labour input into the cropping system, in addition, many farmers do not have the opportunity to leave land fallow for one season to produce the LCC. This method of soil fertility improvement is just one of the many options available to farmers and the exact production system the farmer develops will depend on many other issues, for example, access to inorganic fertilizers, the need for firewood, livestock feed or grain legume production. Farmer evaluations identified research gaps with this technology that are now being investigated in further on-farm experimentation. LCC species, however, still remain a strategic opportunity for the many farmers that have no access to fertilizers or animal manures and who have available land. ---------------------------------------------t DM ha -1 --------------------------------------------- In the recent past the image of agricultural and environmental crises in sub-Saharan Africa (SSA) has become increasingly common. Soil erosion and soil fertility loss are considered to be undermining the productive capacity of the agricultural systems (Giller et al., 1997;Sanchez et al., 1997;Smaling et al., 1997). Within this environment smallholder farmers use a wide range of agro-ecological management techniques, resource management practices and production strategies specific to their environment to minimise risk, cope with change and shocks and to manage the environment (ecological, social, economic etc) they operate within. These can include, for example, agricultural intensification, expanded marketorientation, increased capital and labour investment. Alternatively, farmers have been found to exploit their resource base where constraints are too high, the returns to investment are too low (even negative, as when staple commodity prices plummet during bumper harvests), or environmental conditions too erratically variable for secure investment. Since 1998, a number of collaborating partners working in Tororo district through an adaptive research project, the Integrated Soil Productivity Initiative Through Research and Education (INSPIRE) have been evaluating a range of soil fertility management options with farmers. The collaborators in the INSPIRE initiative which began with the main objective of introducing, developing, on-farm testing and disseminating improved soil fertility management technologies to address the alarming soil productivity problems in Tororo district include, Africa 2000 Network (A2N), Appropriate Technology (Uganda), International Centre for Tropical Agriculture (CIAT), Tororo district Departments of Agriculture and Extension, Farmer group representatives, Food Security and Marketing (FOSEM) project, International Centre for Research in Agroforestry (ICRAF), National Agricultural Research Organization (NARO), Makerere University, Tropical Soil Biology and Fertility Programme (TSBF), and Uganda National Farmers Association (UNFA).During the participatory diagnostic stage of the project, use of legume cover crops and biomass transfer species to improve soil fertility were identified as potential technologies due to their cost effectiveness, appropriateness, simplicity, and multi-purpose nature in meeting the needs of resource poor farmers. In smallholder farming systems of the tropics and sub-tropics, increasing use is being made of legume cover crops (LCCs) and biomass transfer (BT) species as sources of nutrients, particularly nitrogen, for crop growth (Dreschel, et al., 1996;Rommelse, 2001;Buresh and Niang, 1997). This is in part due to the increasing cost and variable availability of inorganic fertilizers at the village level. Less than 5% of the farmers in eastern Uganda use mineral fertilizers and this is usually on an irregular basis (Mirro et al., 2002). In addition, traditional natural fallow is no longer practiced due to the decreasing farm sizes and the availability of animal manure is limited by decreased cattle numbers (Mirro et al., 2002).Six species, Canavalia ensiformis, Crotalaria grahamiana, Dolichos lablab, Mucuna pruriens, Tephrosia vogellii and Tithonia diversifolia were first introduced in two sub-counties of Kisoko and Osukuru. Much work has been conducted on the biophysical performance of these legume cover crop and biomass transfer technologies since 1998 in Tororo (Tumuhairwe, 2002a;Tumuhairwe 2002b;Delve and Jama, 2002b). Beyond agronomic evaluation it is essential to identify opportunities and constraints of each introduced technology, conduct assessments to understand farmers' actual use and management of the technologies, perceived benefits, farmers ideas and perceptions, innovations, and problems and solutions in the use of the technologies (Douthwaite et al., 2002;Bellon, 2001). To address this, a farmer participatory evaluation of these technologies was therefore initiated in December 2001 and January 2002 (after seven seasons) with the main objective of providing a feedback on the performance of the technologies. This paper reports on the findings and analysis of participatory evaluations by 19 farmer groups, involving 234 individual farmers (92 male, 142 female), who had been evaluating through onfarm adaptive research the performance of legume cover crops (LCCs) and biomass transfer (BT) species for soil fertility improvement.Farmer participatory evaluations were conducted using open questions, probing questions and preference matrix ranking. The farmers who participated in the evaluation exercise were purposively selected and belonged to a farmer group who had at least five seasons experience experimenting with LCCs and biomass transfer species. Focus group discussions were used to elicit farmer criteria (negative and positive) used in selection and preference ranking of LCCs and biomass transfer technologies.From the preference rank list of the six species from each group, a frequency table was drawn up of the number of times each species was ranked in a certain position, where one is the most and six is the least preferred species. From this frequency table, the probability of a particular species being ranked in a certain position was calculated, where, Probability = frequency / total number of observations …………….(1)A further calculation was done to produce the cumulative probability of each species, that is, the sum of the probability for that rank and the probabilities for all previous ranks. Further data analysis was done using a logit regression with a Chi-squared test (at 15% level significance) using the Logistic Preference Ranking Analysis Tool for evaluating technology options (Hernandez-Romero, 2000). The preference ranking logic regression allowed the statistical analysis of qualitative preference ranking data and allowed a further separation of species into those likely to be adopted or not.During the focus group discussions and evaluation process, farmers were asked to list the innovations (i.e. what they did differently from the initial aim of the demonstration) for the different species and how this differed from what they had seen in the demonstration sitesFarmers' evaluation of the LCCs and biomass transfer species revealed many positive and negative aspects for each species (Table 1). Some of the positive criteria were, improving soil fertility and providing livestock fodder. More interesting from the research perspective were the negative aspects. For example, Mucuna and Canavalia were notably disliked because their seeds are not edible yet they looked very attractive to eat, and are produced in large numbers even in dry seasons. Mucuna was further disliked for its unsuitability for intercropping and because it can harbour snakes and wild cats if planted near the homesteads. The pest problem on C. grahamiana was cited as a serious set back as the caterpillars that eat the leaves and flowers scare the women and children. Tephrosia also was cited as having a pest problem that leads to flower abortion and hence poor seed formation. Lablab was reportedly having a problem of seed formation while Tithonia was feared to be a potential weed if not managed properly.Criteria for ranking the species' performance were developed and a summary of criteria is given in Table 2. Each group developed its own criteria for ranking, however the four most important criteria for all 19 groups were, yield increase of crop after fallow or intercrop, soil fertility increase, multiple uses of the LCC or BT species and ability to control weeds.Based on the criteria developed with the farmers a ranking analysis tool was used to rank the six species. There was little variation among groups in the rank orders but the overall rank order from the most to the least preferred was Mucuna, Canavalia, Crotalaria, Tithonia, Tephrosia and Lablab (Table 3). Since farmers first experienced most of the LCCs and BT species in the course of the present study, the ranks assigned to some species are a preliminary hint to their adoption potential.Distribution of probabilities of acceptance of LCCs and biomass transfer technologies Table 4 shows the number of times a particular species is ranked in a certain position (acceptance frequencies). For example, Mucuna was ranked in position one seven times, five times in position two, zero times in position three, etc by all the 19 farmer groups. Plotting cumulative probability against the ranking position allows a graphical representation of the acceptance of a technology option (Figure 1). The analysis of cumulative probability versus ranking position showed that for the 19 groups, Mucuna, Crotalaria, Canavalia and Tithonia all had positive intercepts on the y-axis (probability of acceptance), i.e. high probability of being ranked highly by farmers and Tephrosia and Lablab had negative intercepts and are therefore likely to be rejected by the farmers and not adopted (Figure 1; Table 5).A higher slope with a positive intercept on the y-axis means that the technology option has a high probability of being ranked highly by farmers, indicating that they have characteristics that meet farmers needs and therefore should be taken into account while promoting the species. In addition, they are more likely to be adopted. In contrast, a high slope with a negative intercept shows a likelihood of that technology option being ranked often in the last places of the ranking and hence is not liked by farmers.Further analysis of the slope of the regression line and using a Wald chi-square test showed that Mucuna and Crotalaria have low slopes of 0.04 and 0.08 respectively, but with positive intercepts indicating intermediate probabilities of acceptance (Table 5). Canavalia and Tithonia with high slopes of 0.10 and with positive intercepts (differs statistically) have high probabilities of acceptance in accordance with the model used in this analysis. On the other hand Lablab and Tephrosia with high slopes but with negative intercepts indicate low probabilities of acceptance. The analysis of Canavalia was not significantly different (P<0.15) to zero (i.e. there is no difference between the use or no use of the species), indicating likelihood for non-acceptance by the farmers.Farmer innovations with the LCC and biomass transfer species Some farmers indicated that they had tried using the LCCs and biomass transfer species in a different way besides what the researchers had demonstrated during the trials. The ways in which the farmers adapted and adopted the technologies are shown in Table 6. Farmers also invented their own names for some species of the LCC and biomass transfer species they were using (Table 1). Canavalia was locally known as 'Yathipendi' meaning 'medicine for banana' and another group that was dominated by old men identified it as 'Akengu ka Angu' meaning 'trap for the Hyena'. Tephrosia was locally known as 'Yathi fuuko' (medicine for mole rat) or 'Yathirechi' (medicine for fish). Tithonia diversifolia, was locally referred to as 'Mawuwa' but with no particular meaning attached to the name. Mucuna and Crotalaria were known by their botanical names as obtained from the researchers. Farmers' identification of the LCC and biomass transfer species by such names reveals several issues.When faced with many options, framers face complex decisions. This study shows that farmer' assessment of the LCCs and biomass transfer species revealed many positive and negative aspects for each species. The criteria used for selection and the farmers' innovations revealed new research constraints and opportunities. For this farming system, highlighting potential new areas of research, for example, research into suitable niches for the best-bet species (Muhr et al., 2001) and/or identifying varieties that can be consumed by humans, e.g. dual-purpose grain legumes (Ecoregional Alliance, 2001) are important for enhancing the adoption of a technology. This study also confirms the labour constraint with use of LCCs and BT species for soil fertility improvement and therefore a serious constraint in the adoption of the technology (Obonyo, 2001;Tumuhairwe et al., 2002b). Addressing these identified constraints will ensure that future research is relevant to the needs of the farmers and therefore have a higher chance of being adopted.There was no doubt among the farmers that the LCCs technologies work and were better than the traditional practice as far as improving soil fertility was concerned. In terms of costs, it was reported that the use of LCCs and BT species offered a low input technology to the farmers, as most of them could not afford use of inorganic fertilizers especially on the low value crops like maize. Farmers however, observed that the use of LCCs and BT species required a substantial amount of land for production and for sole crop production which is left under fallow with no food crop being produced, high labour for clearing and ploughing in the vegetation, and patience in attaining the results.The fact that food production is the key priority of the farmer means that they are very risk averse and need to produce a food crop every season, so investing present resources in the possibility of future increased production is not necessarily interesting to farmers. As an adaptive research farmer commented, 'Its better to have even one gorogoro tin of maize from a depleted field that was planted with maize than to be guaranteed no maize at all this season by planting a cover crop we can't eat' (Ramisch, pers. Comm..). Despite these constraints, farmers conducting trials on legume cover crops for soil, water and nutrient management in Malawi expressed that through learning-by-doing and doing-by-learning, they learnt that there are some legume cover crops such as Mucuna, pigeon pea, tephrosia, soybeans ground nuts and common beans that improve soil fertility and at the same time be used as a green manure and/or food (Marra et al., 2002;Douthwaite et al., 2002). Recent experiences with farmers using simulation model discussions further provide evidence of the role risk, uncertainty and learning play in the process of adopting/adapting new technologies (Braun, 2001).This study shows that farmers can make use of more than one LCC or BT technology depending on their production objectives and resource endowments. They can observe, compare and decide on alternatives, using criteria drawn from their own experiences. Findings from case studies in Malawi and Zimbabwe also indicate that a broad range of options rather than blanket recommendations (as offered by government extension services) can increase adoption and improve productivity and food security (Marra et al., 2002).The logit preference ranking analysis tool used in this study helps to explain decisions on acceptance or rejection of the technology, based on the criteria and/or farmer group used to choose one technology rather than another. The tool further allows the statistical analysis of qualitative data and a detailed separation of technologies into those likely or unlikely to be accepted, something that is not possible through ranking alone. Information generated from this tool provides essential feedback to the technology development process. This tool has been used to conduct participatory evaluations of cassava, potato, beans and maize varieties in Ecuador and Colombia (Hernandez-Romero, 2000).In the process of technology change and innovation it is essential to understand not only the farmers perceptions but also those of all the stakeholders involved in the research process (Douthwaite et al., 2002;Bellon, 2001). Therefore, the next stage in this adaptive research process involved the systematisation of information, detection of knowledge gaps, and the identification of potential research questions during follow-up community meetings attended by the farmers, extension agents, NGO and CIAT staff. During these meetings the results of the participatory evaluation were discussed and this led to the identification of new research questions that need to be addressed through strategic on-station research; adaptive research conducted by National partners and adaptive research conducted by farmers. The different partners then agreed on the way forward to address these issues:Key farmers to conduct adaptive research on behalf of the community. These farmers will establish a range of experiments, and will be responsible for monitoring the experiments and reporting back to the whole community on the results.Applied research questions to be addressed by National agricultural research partners, through an array of methods from on-station research to on-farm research. Strategic research questions to be addressed by CIAT, TSBF, and other partner international research institutes through an array of methods from, strategic on-station research to on-farm research.In this study, LCC and BT technologies that were introduced to farmers for soil fertility replenishment have been adapted and are being improved through participatory evaluations to include a much wider range of production objectives. The evaluations showed that whilst technologies need to be adapted, a single use technology had little chance of large-scale adoption. This has led to a major rethink by researchers and partners of the methodology and approach taken and the types of research conducted in the project.Whilst technologies exist that increase soil productivity and are profitable for farmers there are many other factors preventing them from adopting the technology. Fallowing the land for example, is not possible where small land sizes or high population densities exist and where seed supply for these legume cover crops is not good. In eastern Uganda, where the population pressure is much lower and where natural fallowing is still part of the farming system, the opportunities for improved fallowing or biomass transfer is much higher. Even so, issues of increased labour requirements for incorporation or collection of biomass are commonly cited by farmers during evaluations.In this dynamic environment farmers assess the different management options available to them and adapt them to fit their own circumstances and production objectives. For example, growing Tithonia on-farm in available niches (around the field boundaries, for example) is one way of over-coming shortage of Tithonia and reducing the labour that would be needed if collecting the biomass from off-farm locations. Innovations in using these legume cover crop and biomass transfer species are very common. This work has identified many adaptations/innovations by farmers not just for increasing crop production but also for pest and weed control, consumption of the seeds and for livestock feeding.The criteria used for species selection and the farmers' innovations provide essential feedback to the participatory action research approach as they reflect the opportunities and constraints of the production systems of the farmers and raise many new areas of research, opportunities of evaluation of new technologies and species and the better targeting of existing information. Young and tender leaves are continuously picked during the entire growing period for sauce. In Tororo, there are several grain legume food crops grown but the major ones in order of importance are cowpea, groundnuts, common bean, simsim, soyabean and green gram. Cowpea was ranked first because it fetches more income than other grains grown in the area.The following criteria was consensually agreed upon and enlisted by the farmers and used to conduct an absolute evaluation: Multiple utilization (e.g. as vegetable sauce, Sumbusa) Pest and disease tolerance Improve soil fertility Good taste and satisfaction obtained when eaten (i.e. ability to stay in the stomach for long) Grain yield obtained (i.e. many grain filled pods) Marketability of the grain seed (i.e. big seed, uniform colour)This present season the farmers are further evaluating the five most promising varieties. In addition, two more varieties breed by Makerere University are being evaluated alongside these. Evaluations will be done at two stages, at 2-3 WAP (for vegetable attributes) and at harvest maturity for grain and biomass yield.The evaluation was done by men and women separately to cater for gender differences. The central objective in conducting a genderized evaluation of the cowpea lines was to make proactive efforts to ensure that women participate and benefit from the technology and capture their innovations. It was anticipated that women face different constraints from men and have different incentives to invest in or adopt cow pea varieties. Based on the evaluation results and field observations, men gave higher score to the local variety compared to the women. However, with regard to the new varieties under evaluation, there wasn't much difference in preference between women and men as reflected also in the evaluation criteria. Furthermore, based on the criteria enlisted by the farmers, the following varieties look promising: IT98K-238-3-3, IT98K-279-3, IT98K-205-8, IT98K-279-3, IT95K-238-3.One of the major problems identified by farmers during participatory research was the lack of seed production from Lablab species due to late flowering and flower abortion, that was severely inhibiting adoption of an otherwise preferred dual-purpose legume option. Thirty-three lines from CSIRO that had early flowering characteristics were introduced with two main objectives of the trials:1. To evaluate the performance of these lines in view of selecting the most promising as regards to improving soil fertility and provision of food 2. To provide grain legumes improvement program with an opportunity to select lines for further testing and use, either directly as varieties or as source of breeding materials.In the 2002b Nitrogen (N) is one of the major limiting nutrients to crop production in Uganda and is depleted at faster rates that replaced. Consequently, yields at farm level are less than 30% of the expected potential.Paradoxically, the majority subsistence farmers are poor to afford use of mineral fertilisers but improved fallow have been reported economically feasible in such conditions. Therefore, a study was initiated in Tororo district, eastern Uganda (i) to determine mineral N contribution of C. grahamiana and M. pruriens short-duration fallows compared with farmers' practices of natural fallow, compost manuring and continuous cropping, (ii) sampling period that closely related to maize grain yield was also determined and also (iii) whether improved fallow provided adequate mineral N for optimum grain yield compared to farmers' practices. It was noted that improved fallows increased mineral N at Dina's site during fallowing (at 0 week sampling), and in the first and fifth week after incorporating their biomass than farmers' practices. For instance, at harvesting fallows (0 week sampling), C. grahamiana and M. pruriens had 12.68 and 12.97 mg Kg -1 N compared to 6.79 and 7.79 mg kg -1 N from following natural fallow and continuous cropping respectively. However, no significant increase was realised at Geoffrey's site at any of the sampling dates attributed to low biomass yield and incorporated. C. grahamiana increased grain yield by 29.3% (Dina's site) and 56.6% (Geoffrey's site) and M. pruriens by 36.0% (Dina's site) and 27.2% (Geoffrey's site) compared to natural fallow with -11.9% (Dina's site) and 17.4% (Geoffrey's site) then compost manure -9.6% (Dina's site and 0% (Geoffrey's site) in relation to continuous cropping as a bench mark. Supplementing the land use systems LUS (C. grahamiana, M. pruriens, natural fallows, compost manure and continuous cropping) with inorganic N fertiliser as urea significantly increased grain yield in all except C. grahamiana at both sites. There were two peaks on mineral N. The first and major peak occurred in the third week dominated by NO 3 --N and the minor one in the tenth week with NH 4 + -N prominent consistent at both sites. Mineral N in the fifth week after incorporating biomass was most closely related to grain yield followed by sampling at planting (0 week).The second Masters thesis (Comparison of the effects of Mucuna pruriens, lablab purpureus, canavalia ensiformis and crotalaria grahamiana on soil productivity in Tororo district eastern Uganda) was submitted in September 2002, the abstract form this thesis is reproduced here.The effect of green manures, Mucuna, Lablab, Canavalia and Crotalaria on soil fertility and productivity in Tororo District, Uganda.There is much concern over the declining crop yields over much of sub-Saharan Africa, and has largely been blamed on declining soil fertility, since increasing population has rendered traditional shifting cultivation and long-term fallowing, less practical. Strategies such as mineral fertilizer application, use of manure (compost and animal) and green manuring have been shown to sustain and/or increase soil productivity. Mineral fertilizers restore lost or limited soil nutrients fast, but are expensive for most farmers and do not improve soil organic matter. Similarly, compost and animal manure use is limited by the quality of the composted and/or feed material as well as the labour requirements for their preparation and application to farm fields. Legume cover crops, which are produced on the field with the crops and later incorporated into the soil to provide plant nutrients upon decomposition, could be a viable option for soil productivity improvement, especially in smallholder low-input agriculture systems. Whereas the technology has been widely adopted in the tropics, it is still low in Uganda, probably due to lack of awareness and performance data. This study was therefore planned to demonstrate the value of legume cover crops on soil productivity improvement and to determine and compare the economic viability of four legume species (Mucuna pruriens, Crotalaria grahamiana, Lablab purpureus and Canavalia ensiformis) in order to give sound recommendation for wider adoption of the technology. To be of relevance to farmers, six on-farm trials (each farmer as a replicate) were set up in two sub-counties Kisoko and Osukuru, and another on-station trial at the District Agricultural Training Centre (DATIC) with four replicates, in Tororo District, eastern Uganda. In August 2000, maize (cv. Longe1) was established on five-5 x 5 m plots and at first weeding stage (4WAP), the four legume cover species were each planted between maize rows in all the plots except the control (maize monocrop). After harvesting maize in December 2000, the cover crops continued to accumulate biomass for two more months, and in February 2001, the above ground biomass of the cover crops and of weeds was harvested, fresh weight taken, sampled for drymatter determination and incorporated into the soil during land preparation for the long rain season in March 2001. Production costs that were different for different treatments were estimated and recorded during the experiment. Maize yields were also recorded to allow computation of the returns from legume cover crops using marginal rate of return of non dominated treatments, as a basis for recommending the cover crop species to farmers. Results indicated significant (p<0.05) maize yield increases for Crotalaria and Lablab treatment of 96.4% and 69.6 % respectively on farmers' fields in the second season (after legume biomass incorporation) and non-significant yield response to all legume cover crops on-station in both seasons, were obtained. The significant maize yield response to Crotalaria and Lablab on-farm and not on-station was probably due better synchrony of nutrients released from their biomass on an initially poorer soil at the on-farm compared the relatively better soil on-station. The analysis of costs and benefits revealed favourable marginal rates of return to Crotalaria, Canavalia and Mucuna of 246, 120 and 30.4% respectively and were all recommended for adoption with more emphasis on Crotalaria.Tumuhairwe, J.B 1 ., B. Jama 2* , and R. Delve 3 , M.C. Rwakaikara-Silver Crotalaria grahamiana and Mucuna pruriens improved fallows are gaining popularity among smallholder farmers in Uganda to address soil fertility decline. The technology supplies nutrients and increases crop yields but its economic viability is uncertain in eastern Uganda. Therefore, two researchermanaged experiments were established in Tororo District, eastern Uganda to determine the financial benefits of the C. grahamiana and M. pruriens improved fallow compared to farmers' practices of natural fallow, compost manure and continuous cropping. Higher returns to land were obtained from improved fallow compared to farmers' practices. C. grahamiana realised US$267.4 (Dina's site) and $ 283.2 (Geoffrey's site), and M. pruriens had $284.1 (Dina's site) and $248.7 (Geoffrey's site) compared to natural fallow $223.3 (Dina's site) and $274.3 (Geoffrey's site), compost manure $70.9 (Dina's site and 114.2 (Geoffrey's site) and continuous cropping $314.2 (Dina's site) and $314.2 (Geoffrey's site) per hectare. Improved fallows saved on labour compared with continuous cropping and compost manure except for natural vegetation fallow. Higher returns to labour were obtained through use of improved fallow than compost manure and continuous cropping. Returns to labour of $0.54 day -1 were obtained for compost manure (at Dina's site), which is less that the wage rate at $0.57 day -1 indicating a loss in labour invested.The second Masters thesis (An assessment of the profitability and acceptance of alternative soil improvement practices in Tororo district, Uganda) was submitted in September 2002, the abstract form of this thesis is reproduced here.Agricultural production in Eastern Uganda is declining due to increasing population pressure on the land. A resultant feature is the dependence of soils on external inputs to attain acceptable crop yields. Resource-poor smallholder farmers, who form the majority of the farmer population in this area, can typically ill-afford recommended levels of inorganic fertilizer use to replenish lost nutrients. Alternative options to expensive and often unavailable inorganic fertilizer use for this small scale farmer population include the integrated use of inorganic fertilizer and organic inputs such as legume cover crops and biomass production shrub and tree technologies. These technologies were incorporated into the farming systems in Eastern Uganda, Tororo district, in 1998 through farmer groups. An economic evaluation of 10 researcher-designed-farmer managed maize trials using Mucuna pruriens and Canavalia ensiformis fallow and Tithonia diversifolia biomass land use systems were conducted. The profitability was determined using gross margins, after which modelling produced the optimal land use system. A survey of 108 respondents was also conducted to determine the acceptance and farmer-perception of 8 previously exposed shrub and tree species. The economic evaluation favoured the use of Integrated Nutrient Management of soil amendments. The 100% incorporation of Mucuna produced the highest benefits of 185,641/= ha -1 as opposed to the net benefit of 134,901/= that would be produced in the optimal solution from 0.6 ha using 191 labour days. The application of 0.91t ha -1 + N biomass system would produce the highest benefits of 445,744/= ha -1 with an optimal net benefit solution of 342,080/= on 0.8ha using 263 labour days. The survey results showed that in the sample size, the acceptance rate was 53 percent. The age and area under shrub were significantly different (0.01) across accepters and non-accepters. The cultivated area (0.1) and employment activities (0.05), institutional support such as belonging to groups and number of extension visits significantly also differed. Alternative uses of shrubs and trees, use of other complimenting inputs and perceptions of the soil fertility were highly significant across acceptor category. Farming experience and use of farmyard manure were not significant. Sesbania sesban, and Mucuna pruriens were found to be the most popular shrubs (36.69%, and 20.6% respectively) and problematic (36.22%, and 25.20% respectively). Popular uses were weed suppressant uses (17.5%) and fuel wood production (23%) for 7 out of 8 shrubs. Major reported problems were the increased labour demands, (21.5%), pest and vermin association (25.3%), and access to planting material and seed (26.7%). Further economic studies that will determine the optimal levels at which the incorporation of livestock management systems into the cropping systems using integrated nutrient management options are recommended. Farmer designed-farmer managed trials would establish preferred farmer management practices to ensure sustainability of these land use systems.Over the last 10 years the image of agricultural and environmental crises in sub-Saharan Africa (SSA) has become increasingly common. Soil erosion and soil fertility loss are considered to be undermining the productive capacity of the agricultural systems (Giller et al., 1997;Sanchez et al., 1997;Smaling et al., 1997). These problems have been ascribed to many different causes, social, economic, biological and physical. Many authors have also highlighted concern over the increasing land degradation in the highlands of East Africa (e.g. Hilhorst and Muchena, 2000;Farley, 1995;Getahun, 1991) where increases in agricultural production in recent decades have been achieved through intensification of existing agricultural practices and through expanding the cultivated areas of land, especially in fragile environments. Soil degradation, soil erosion and loss of soil fertility have been widely quoted as resulting from these intensive and extensive agricultural production systems.Blaming smallholder farmers for this degradation is over simplistic in the least. Furthermore, tropical agricultural production systems are characterized by dynamic features, resilience and many examples of modified production practices to cope with and adjust to changes (Brookfield and Padoch, 1995;Farley, 1995;Goldman, 1995). Smallholder farmers use a wide range of resource management practices and production strategies specific to their agro-ecology to minimise risk, cope with change and shocks and to manage the environment (ecological, social, economic etc) they operate within. These can include, for example, agricultural intensification, expanded market-orientation, increased capital and labour investment. Alternatively, farmers have been found to exploit their resource base where constraints are too high, the returns to investment are too low (even negative, as when staple commodity prices plummet during bumper harvests), or environmental conditions too erratically variable for secure investment. Where purchased inputs or labour are scarce, mining the soil's nutrient capital resource can appear to smallholders as good economics and an acceptable cost of agricultural production.This paper uses evidence from two sites in eastern Uganda and western Kenya to investigate land management, land use changes, and the policy environment within which smallholders have to operate, and assess their impacts on smallholder farmers' production strategies. Both sides of the border have similar agro-ecosystems and cropping systems, with eastern Uganda through to western Kenya occupying a gradient with changing soil types, from the alfisols in Uganda to humic nitisols in western Kenya, increasing agricultural production and also increasing population densities from east to west. This has resulted in a range of land use systems to manage this gradient.Ugandan and Kenyan national research institutions (in collaboration with international agricultural research centres) have developed an array of technologies that can effectively address local production problems, for example, improved banana and maize varieties for various agro-ecological zones, as well as, legumes and cover crops that improve soil fertility and provide fodder. Many of these technologies have, however, not been disseminated adequately to farmers and have, therefore, little impact at the farm level. The need for improved dissemination of knowledge to farmers has been identified by many studies (e.g. Onesimus et al., 1999). To do this, it is increasingly being recognised that the best approach is one in which farmers, the local administration, and the community participate actively.Examples of technologies developed in the region by collaborative research between farmers and scientists include: Phosphorus replenishment. Phosphorus is a major limiting nutrient to much of the region's crop production due to low soil P availability and many soils' high P-fixing capacity, especially in western Kenya. The socio-economics of smallholder production limit the feasibility of using fertilisers, but combining organic residues with locally available, low-cost rock P, can improve P availability to crops. As well, research on a P-fixing Nitisol in western Kenya has shown that soil P replenishment using seasonal additions of small rates of P fertilisers could be attractive to some small-scale farming systems (Nziguheba, 2001). Seasonal additions of 25 kg P ha -1 increased maize yield with gradual replenishment of soil P. Smaller rates of 10 kg P ha -1 contributed to soil P depletion, while large seasonal applications of 150 kg P ha -1 resulted in low efficiency of applied fertilisers.• Legume cover crops. In regions where natural fallowing is still practiced (as in Eastern Uganda), green manure species like Mucuna pruriens and Canavalia ensiformis increases the following maize yields (Delve and Jama, 2002a). In addition, the significant increases in associated maize stover production increased options available to farmers, such as using it for livestock feed or bedding, soil erosion control, compost making, or mulching the banana crop. Delve and Jama (2002a) also found that incorporating 50% or 100% of the in-situ produced biomass did not result in significantly different increases in maize grain and stover yield. This would allow farmers to use 50% for incorporation and the remaining 50% for livestock feed, sale to other farmers, or to produce hay for dry season feed. Increasing the resource management options and therefore the production options of the farming enterprise is critical where land sizes and the area available for non-food crop production are small, and where cash is not readily available to buy inputs for crop and livestock production.• Biomass transfer. In both western Kenya and eastern Uganda application of high quality local materials, such as Tithonia diversifolia, has shown good potential to increase productivity. Work in western Kenya, supplying a constant rate of 15 kg P ha -1 through combinations of Tithonia leaves low-quality maize stover and triple super-phosphate (TSP), showed that maize yields increased between 18-24% as the share of P contributed by Tithonia in the residue-fertiliser mix was increased above 36%. The results indicate that a high quality organic input can be more profitable than using inorganic P, and comparable to or more effective than inorganic P in increasing P availability in the soil. Work in Uganda combining Tithonia with fertilisers also obtained the greatest benefits by maximising the proportion of Tithonia in the mixture (Delve and Jama, 2002b).Whilst technologies exist that increase soil productivity and are profitable for farmers there are many factors limiting technology adoption. The fact that food production is the key priority of the farmer means that they are very risk averse and need to produce a food crop every season. Even where land is not apparently scarce, investing present resources in the possibility of future increased production is not necessarily attractive to farmers. As a research farmer in Kenya commented, 'Its better to have even one gorogoro tin of maize [from a depleted field that was planted with maize] than to be guaranteed no maize at all this season by planting a cover crop we can't eat'. Issues of increased labour requirements for incorporation or collection of biomass are also commonly cited by farmers during evaluations of the organic technologies. In western Kenya there are even examples of teachers using 'free' labour of children coming to school to harvest Tithonia for use on school plots.The implicit assumption of most agricultural research is that farmers' current resource management decisions are not the optimal ones, and that providing them with 'better information' would lead them to better choices. However, without understanding farmers' priorities and constraints the rationality of their current decisions will also be misunderstood. Similarly, by ignoring farmers' existing knowledge (or not accurately locating the gaps in that knowledge) the impacts of improved land management technologies will be minimal. Agricultural knowledge, access to new sources of information, and control of resources can vary considerably within a given community, especially across axes of difference such as gender or age. Technologies that are designed collaboratively by researchers, extensionists, and farmers are more likely to correctly target the socio-economic and agro-ecological niches where they will be most relevant.Innovations in using these soil fertility management technologies are very common. A recent survey identified many adaptations/innovations by farmers using cover crop and biomass transfer species not just for increasing crop production but also for pest and weed control, consumption of the seeds and for livestock feeding (Nyende and Delve, 2002). Farmers assess the different management options available to them, and adapt them to fit their own circumstances and production objectives. Growing Tithonia onfarm in available niches (around the field boundaries, for example) is one way of overcoming shortage of Tithonia and reducing the labour that would be needed if collecting the biomass from off-farm locations. For other farmers, the rapid decomposition of Tithonia makes it 'more like a fertiliser' (i.e. immediate effect, with little residual benefit) and therefore less attractive than farmyard manure (compost of animal, household, and crop wastes) which 'builds the soil' for the long term.Recognition that innovation comes from multiple sources means that technology development must involve potential users from very early in the design process. To support this, extension must be more intimately linked with research to ensure that nascent technologies take fuller account of farmers' existing knowledge, practices, and priorities. 'Dissemination' would be of prototypes fully intended for modification or rejection by farmers and not of 'finished' products. However, by treating technology itself as politically neutral -i.e.: without knowing who benefits from existing practices, or who will likely benefit from changes -policy recommendations relating to soil fertility management will remain too vague to truly assist policy-makers, or be delivered through inappropriate channels to sectors unable to make use of them.While some of the constraints to crop production and examples of options available for alleviating soil productivity problems have been discussed at the farm level, many of the constraints facing farmers come from external forces, such as the (mis-) functioning of input and output markets, which can only be affected by modification of the 'policy environment'. For example, the bumper harvest reported in Kenya and Uganda in the 2001 short-rain season led to sale prices of maize that were often below production costs. In such situations, farmers face the prospective of losing money if they sell their maize to generate cash, but there is also no incentive for them to invest in their agricultural enterprises given the policy environment they operate within. Clearly, innovations need to address food security and livelihood sustainability, not just increased production as a good in its own right. Policy interventions that would rationalise input and output markets, and buffer smallholders from their volatility, should have as their goal a) increasing farmers' opportunities to innovate, and b) making investments back into agriculture attractive. One way in which such support could be given to smallholders would be by increasing investment in linking research, development, and extension with farm communities. In Kenya, the collapse of the formal extension network over the last five years has led to a shift towards farmer extension and farmer-to-farmer training through for example, farmer field schools. This increased reliance on information diffusion through social networks requires a better understanding of the role of social capital in innovation. In contrast, in Uganda, a newly privatised extension service is being piloted in test districts across the country, where parish level farmer forums feed through sub-county and counties to the district, which then contracts extension providers to provide the demanded services. This demandled process has the potential to allow smallholder farmers increased access to markets, agricultural inputs and extension services and to improve access to information and technologies through the contracting of private sector service providers. This in turn will lead towards a more market orientated smallholder production sector.The shortcomings of traditional economic impact assessment (IA) Folklore in the CGIAR system tells of a golden period in the 1970s when the only financial constraint was the capacity to spend the money wisely. Back then, donors believed that \"if a group of competent scientists were based in a developing country, were provided with excellent facilities, and were isolated from political pressure for several years, they were bound to generate useful new technologies\" (Horton and Prain, 1989, p. 302). Those days have long gone. Not surprisingly donors started to perceive that isolation made CGIAR centres unresponsive to the needs of farmers and agro-industries, and started to demand evidence of priorities set jointly with the intended beneficiaries of the research, research impacts and the efficiency of research investments. In response to this pressure CGIAR centres invested much effort in the 1980s and 1990s in developing impact assessment methods (Ekboir, 2002). Much of this work was driven by the dominant social science approach in the CGIAR system, which remains grounded in traditional agricultural economics (Horton, 1997). These approaches rely on establishing a mechanical causal relationship between the costs and benefits of research. 4 'Best practice' economic impact assessment is represented by the book Science under Scarcity (Alston et al., 1995), which is dismissive of other, less linear approaches as being \"unlikely to yield any meaningful indications of the economic effects of research. … Therefore, they are not useful for informing allocation decisions.\" (p.501-2).We contend that the predominance of traditional economic IA methods in the CGIAR system, often to the exclusion of a whole gamut of evaluation approaches developed in the field of evaluation, does not help solve donors' legitimate concerns about research relevance and impact. This is for two main reasons. Firstly, economic IA methods focus largely on ex-ante IA and then ex-post IA, but have little to offer in the area of monitoring and evaluation (M&E), despite M&E being identified as being important in helping research projects actually achieve impact. Secondly, economic IA, based as it is on linear models that link research inputs to outputs, is only valid if: 1) the causal link dominates from start of research to the measurement of impact; 2) there are no other factors affecting adoption and impact; 3) chance has no influence; and 4) inputs and impacts can be measured to an acceptable degree of accuracy (Ekboir 2002). In practice these assumptions can hold, as Table 5 shows, only for research activities developing 'minor' technological changes intended for use in 'simple' systems. Breeding of new plant varieties for irrigated areas that are already growing improved varieties of that particular crop, is one of the very few CGIAR research activities that would qualify. New varieties are simple to use because they are not new technologies but rather minor improvements of existing techniques along well known technological trajectories. Hence, the need for user modification and innovation, and therefore the unpredictability and non-linearity that this brings to IA, is limited. But since market and policy changes can affect adoption decisions in unforeseen ways, even relatively simple cases like the one described, are becoming increasingly complex due to globalisation and deregulation of agricultural markets. Complex \"hard-to-employ\" Natural resource management in simple systemsSimple \"easy-to-employ\" Plant breeding for irrigated systems Participatory varietal selection in rain-fed systems Key:Where linear innovation approach and conventional impact assessment could eventually workWhere complexity requires close research and user interaction and assumptions underpinning economic impact assessment breakdownBox 1 gives a case study of a 6-fold increase in grain production in MERCOSUR 5 that was the result of the interaction of three technologies with social innovations and explains why impacts cannot be attributed to research outputs alone. The case study also shows that ex-ante impact assessment prioritised the wrong research area, illustrating the point that ex-ante impact assessment can only recognise technological trends once they have begun to emerge. Hence, institutions can only establish research programs in relatively known fields. If new trends are to emerge, researchers must be allowed to explore less known areas of research.Box 1: A case study of impact where traditional economic IA does not work (from Ekboir, 2002) In the 40 years between 1961 and 2001 production of maize, sorghum, sunflower, soybeans and wheat in MERCOSUR increased from 23 million tonnes to 152 million tonnes. The increase came about by farmers adopting three interdependent technologies: the introduction of soybeans in late 1960s, zero tillage and improved germplasm. Soybean production led to an intensification of agriculture, which caused serious soil degradation. A number of technical solutions were proposed to solve the problem, including zero tillage and terracing. At the time, researchers identified terracing as the more promising option, and as a result soil conservation projects neglected work on zero tillage. Nevertheless, by 1985 viable zero tillage systems had been developed by a network of agents, including agrochemical companies, a few public sector researchers, farmers and agricultural machinery manufacturers. In the late 1980s researchers and farmers, with support from Monsanto, created associations to promote zero tillage. Adoption, however, remained low until the early 1990s because the herbicide glyphosate (a key component of the package) was expensive. Then, a change in corporate policies helped bring about a fall in price from US$ 40 per litre to US$ 10 per litre. The new relative prices combined with a very effective diffusion policy organized by the association caused adoption to explode (Ekboir, 2001). Zero tillage reduced production costs, reversed soil erosion and allowed an expansion of agriculture into previously marginal lands. Without zero tillage, grain production would have had to be abandoned in many areas. The impact of these technologies cannot be separated. Without zero tillage, the impact of improved germplasm would have been very small, as zero tillage was necessary to stop soil erosion and improve water management. At the same time, new and improved germplasm increased the profitability of zero tillage, fostering adoption. But adoption only exploded when a key input produced by a private firm became affordable.Economic IA assumes a mechanical link between research outputs and the benefits, and then attempts to separately attribute impact to the different components of the package. However, this is not possible in cases like this characterized by multiple interactions and feedback loops among several physical and social components and agents. Hence, traditional economic IA is not able to evaluate the research that contributed to the impact, because, for example, without the reduction in the price of glyphosate the impact would have been small. But the price reduction was completely unrelated to plant breeding or development of zero tillage.In addition to the failure of ex post economic IA, ex ante impact assessment also failed by wrongly prioritising terracing as the most viable technical solution to soil degradation. As a result resources were siphoned off that might have otherwise hastened the adoption of zero tillage. Only after terracing proved to be unsustainable was zero tillage recognised as the best option.Complex adaptive systems are characterized by three features: several interactions among agents and processes, strong feedback loops and intrinsic randomness. Because of these three features, these systems are essentially unpredictable in the long run, even though limited predictability is possible in the short run. Although outcomes cannot be predicted, key factors that influence the probability of success of agricultural innovation processes have been identified. These are the emergence of strong and flexible networks, the adoption of participatory research and diffusion methods where farmers play a key role, flexible evaluation and monitoring routines in public research institutions and access to internationally generated information (Ekboir and Parellada 2002). Effective monitoring and evaluation routines of these factors that result in rapid corrective measures can greatly increase the chances of large impacts.In addition to traditional IA, the CGIAR system needs innovative M&E approaches that aid a continuous redesign of on-going research projects (including 'learning by doing', 'learning by learning', mapping of innovation networks, creation of organizational capabilities and adaptive management). The importance of M&E to good, adaptive, project management is recognised as key to successful Integrated Natural Resource Management (Sayer and Campbell, 2001). Furthermore, donors at the February 2002 CGIAR Impact Assessment Conference urged centres to focus more on M&E that contributes to institutional learning and change, and less on ex-post IA of successes for publicity purposes. The following are three cases studies of recent and on-going M&E exercises to show the types of knowledge that this work can produce, and how it can feed back into the research and priority setting process. We then follow with a discussion of how inclusion of M&E can build the foundation for more plausible expost IA and be used as an essential tool in priority setting.Conceptual map of the innovation process All three case studies described in this section implicitly assume the conceptual map of the technology development and adoption process shown in Figure 1, and therefore the case studies are described with reference to the model. The model recognises four phases in the innovation process: Development Phase-Innovators (e.g., researchers, farmers, input suppliers or other agents working together or in isolation) are permanently searching for new technological or economic alternatives to achieve their objectives (which may include improved livelihoods for farmers or professional recognition for researchers). Problem diagnosis with the intended target group(s) is part of this process. When an alternative is identified, the innovators develop 'best bet' integrated solutions.Start-Up Phase-The network of early developers take these 'best bet' options and demonstrate them to individual and/or a network of farmers, in the hope that farmers will see that at least some aspects hold out a 'plausible promise' of being benefit to them, sufficient to motivate at least a few to contribute their own time and land in experimenting.Adaptation Phase-Experimenting farmers and other agents work together to adapt and refine the 'plausible promise' into something better; something that is seen to work and make sense to the wider community;Expansion Phase-Adoption levels expand as the community begins to adopt their locally-constructed solution(s). This might be an integrated package and/or a single component of the 'best bet' options originally introduced.While necessary in all phases, M&E is particularly important in the adaptation phase to help ensure the innovations and farmer adaptations can be captured and incorporated into the research process.Implicit to this conceptual map is the premise that once developed, a complex technology that is widely adopted in a pilot site, will scale-out to other, similar, communities through multi-actor interactions. However, scaling-out can be accelerated by a properly designed extension approach that speeds up both the knowledge spread and the experiential learning that is necessary to construct the technology in communities elsewhere.In late 1998, the Tropical Soil Biology and Fertility Institute of the International Centre for Tropical Agriculture (TSBF-CIAT) introduced legume cover crop (LCC) and shrub species, proven to improve soil fertility, into two sub-counties of Tororo District, in eastern Uganda. These legume species were: Canavalia ensiformis; Crotalaria grahamiana; Dolichos lablab; Mucuna pruriens; Tephrosia vogellii; and Tithonia diversifolia. TSBF-CIAT began by setting up several on-farm trials, together with 40 participating farmers. The purpose of these trials was to validate and demonstrate the effectiveness of the LCCs and shrubs as, for example, cover crops to control weeds, or to improve soil fertility, by, in some cases, biomass transfer (BT) from one field to another. These activities were backstopped by project field officers, as well as, by the district extension services of the government. To reach more farmers a range of approaches were implemented, extension agents were trained on the use and management of the technology, innovative farmers were identified in each sub-county and trained, farmer-to-farmer extension formed an important component of the program. Many study and exchange tours were organized to enhance farmer-to-farmer learning and adoption of the technologies promoted. By the end of 2001 over 2000 farmers had established their own evaluation trials as a result of extension visits and farmer-trainer visits and from exchange visits to demonstrations sites.TSBF-CIAT conducted a farmer participatory evaluation of the LCC and BT species after seven seasons in December 2001 and January 2002 with 21 farmer groups, representing 234 farmers (92 male, 142 female). The farmer groups were purposively selected on the basis of having several seasons' experience with the legumes and their management. Group discussions and key informant interviews were then held to:• Establish farmers' assessment of the legume species for soil fertility improvement;• Identify farmer innovations with respect to the use and management of the legumes; • Identify farmers' evaluation criteria when comparing between legumes;• Conduct a matrix ranking based on these criteria.Farmers identified a number of positive and negative characteristics of each legume (Table 6). Some of the positive aspects correspond to innovations made by farmers during their trials (Table 7). Generally, the innovations show that farmers are seeking to increase the benefits of the technologies by finding alternative and dual-purpose uses for the legumes, other than for soil fertility or weed suppression. These include attempting to: eat the seed and leaves in sauce (Lablab, Mucuna), to control crop pests and diseases (Crotalaria, Canavalia, Tephrosia), catch fish (Tephrosia) and curing human ailments (Tithonia). Other innovations, for example, border planting, are designed to reduce the cropland taken by the legumes. An indication that farmers had learnt to value the legumes was that some groups gave them local names. One group called Canavalia 'Yathipendi' meaning 'medicine for banana' while another group, that was dominated by old men, identified it as 'Akengu ka Angu' meaning 'trap for the Hyena'. Tephrosia was locally known as 'Yathi fuuko' (medicine for mole rat) or 'Yathirechi' (medicine for fish). Tithonia diversifolia, was locally referred to as 'Mawuwa' but with no particular meaning attached to the name. It should be noted, that in some cases farmers knew the species before TSBF-CIAT established the trials but were not aware of their potential uses and that some of the names, innovations and discoveries occurred outside the learning cycles that took place as a result of the project trials. Leaves used for treatment of stomach ailments and fevers Farmers were also asked to make explicit the criteria they used when evaluating the legumes Table 8) and the overall farmer ranking, developed using a ranking analysis tool with the 21 groups, is shown in Table 9. The rank order from the most to the least preferred was Mucuna, Tithonia, Canavalia, Crotalaria, Lablab and Tephrosia. For the 21 groups a cumulative probability was plotted against the ranking order given by each group (Figure 2). The area under the lines for the different LCC and BT species is directly related to its ranked popularity. For example nearly all the groups rated Mucuna either first or second, giving a large area under the Mucuna line, while most farmers rated Tephrosia either fifth or sixth giving a much smaller area under the Tephrosia line. What Figure 2 shows is that Mucuna is clearly the most popular, Lablab and Tephrosia are almost universally unpopular and have a low probability of being accepted in any village, while Crotalaria, Canavalia and Tithonia are moderately popular with little to distinguish between them. This analysis has been confirmed using a logic regression analysis (Nyende and Delve 2002). Discussions with farmers during the group assessments and ranking exercises, as well as open and probing questions, gave insights into the constraints to farmer adoption. Fallowing the land is not possible where small land sizes or high population densities exist and where seed supply for these legume cover crops is not good. In eastern Uganda, where the population pressure is much lower and where natural fallowing is still part of the farming system, the opportunities for improved fallowing or biomass transfer is much larger. Even so, farmers commonly cite difficulties in finding the labour required for collecting or incorporating biomass. Also, many farmers are very reluctant to use land and effort without producing a crop, even if future benefit justifies the investment. This is because most farmers' main priority is food production, and they are very risk adverse. As an adaptive research farmer commented, 'Its better to have even one gorogoro tin of maize [from a depleted field that was planted with maize] than to be guaranteed no maize at all this season by planting a cover crop we can't eat' (Ramisch, pers. Comm.).The next stage in this adaptive research process involved the systematisation of information from the M&E, detection of knowledge gaps, and the identification of potential research questions during followup community meetings attended by the farmers, extension agents, NGO and CIAT staff. During these meetings the results of the participatory evaluation were discussed and this led to the identification of new research questions that needed to be addressed. For example, Lablab was identified as a very promising multi-purpose legume but the variety the community had was not producing seed. As a result new photoperiod insensitive and early flowering germplasm from Australia and Africa is now under-going onstation evaluation.After identifying new research questions the different partners then agreed on how to address the issues. They did this by: • Identifying key farmers to conduct adaptive research on behalf of the community. These farmers will establish a range of experiments, and will be responsible for monitoring the experiments and reporting back to the whole community on the results. • Applied research questions to be addressed by National agricultural research partners, through an array of methods from on-station research to on-farm research.• Strategic research questions to be addressed by CIAT, TSBF, and other partner international research institutes through an array of methods from, strategic on-station research to on-farm research.The most important outputs of the M&E process was the identification of the criteria used for species selection and the farmers' innovations. Both provided essential feedback to the participatory action research approach as they reflect the opportunities and constraints of the production systems of the farmers and raise many new areas of research, opportunities of evaluation of new technologies and species, and the better targeting of existing information. The M&E has shown that farmers adapted technologies introduced primarily for soil fertility replenishment in an attempt to fulfil a much wider range of production objectives, leading to the conclusion that a single-use technology had little chance of large-scale adoption. This has resulted in a major rethink by researchers and partners of the methodology and approach taken and the types of research conducted in the project.Striga hermonthica is a parasitic weed that attaches itself to the roots of cereals (e.g. maize, sorghum, millet and rice), diverting essential nutrients and leaving the host stunted and yielding little or no grain. The weed is the severest biological constraint to cereal production in sub-Saharan Africa, infesting almost 21 million hectares of land causing millions of dollars of damage (Sauerborn, 1991). Farmers world-wide call it 'witch' weed, because it does most of its damage before it emerges from the soil.Research at IITA and elsewhere is showing that Striga control is possible using an integrated approach that attacks Striga from several sides at the same time. A key component of this Integrated Striga Control (ISC) approach is the use of a legume crop (e.g., soybean, cowpea, groundnut) that induces a high proportion of Striga seeds to germinate, which then die because they cannot parasitize legumes. This is called 'trap cropping'. To be effective, legume trap crops must be planted much more closely than farmers usually plant their legumes, and should be used together with Striga-resistant cereals, seed cleaning to remove Striga seed, crop rotation, weeding of the Striga plants before they set seed, and improved soil fertility.Since 1999, a research project at IITA has been working in four villages in Northern Nigeria using participatory research approaches to develop locally-adapted integrated Striga control (ISC). The villages where chosen on the basis of having severe Striga problems. Two group meetings were held, first to carry out a problem consensus to rank Striga in relation to other problems, and then to design experiments to evaluate the options for Striga control. The R&D team has provided training to improve farmers' understanding of Striga. The work began with 19 participating farmers (Schulz et al. in press).M&E has been built into the project from early on, based on the project impact pathway shown in Figure 3. The impact pathway describes how the project expects the output-validation and adaptation of ISC options in farmers' fields-might lead ultimately to the project goal of improved livelihoods for the 100 million people in Africa that are affected by Striga. The shaded boxes are the intermediate outcomes that the project is monitoring. The unshaded boxes will be evaluated in the ex post impact assessment some time after the end of the project.The project is using two published approaches to monitor and evaluate the delivery of the intermediate outcomes shown in Figure 3. The first is the 'Follow the Technology' (FTT) approach (Douthwaite et al. 2001;Douthwaite 2002) that sees technological change in general, and early adoption in particular, as an evolutionary process in which stakeholders generate novelties (i.e., make modifications; innovate), select those that appear to work and promulgate the results. The Follow the Technology approach involves, as the name suggests, following new technologies and knowledge as they are adopted. The FTT approach focuses on identifying modifications, selection decisions (i.e., whether farmers decide to adopt a modification), and promulgation processes. Key to the direction and nature of an evolutionary process is the environment, hence the FTT approach pays particular attention to seeking explanations for novelties generated, selection decisions made and the nature of promulgation paths to understand the socioeconomic and cultural factors affecting farmers' learning and decision making processes. By paying particular attention to who is, and is not, modifying and adopting, as well as identifying conflicts arising from adoption, the FTT approach is able to identify negative as well as positive consequences.The project will use the Sustainable Livelihoods Framework (SLA) (Scoones, 1998), summarised in Figure 4, to guide an evaluation of whether adoption of ISC is having any impact on peoples' livelihoods. This will be done by constructing case studies of individual households, purposively selected to be representative of poor, medium and rich households in the four villages. From October 2001 to January 2002 a survey was carried out to identify farmers who had adopted at least one component of ISC from the participating farmers. A total of 245 expansion farmers were identified in this way. The positions of the 44 participating farmers' experimental plots and the subsequent 'expansion' plots were then marked using a hand-held GPS and plotted using the geographic information systems (GIS) program ArcView (ESRI, 1999). A data sheet was completed to record what was planted in the fields, and modifications made to the recommended package shown in Figure 3. From February to June 2002 an in-depth survey was then carried out of a random sample of 149 of the participating and expansion farmers. The survey sought explanations for farmers' adoption and modification decisions, his or her understanding of ISC, and to find out where the farmer received the technologies from, and who he or she has passed them on to. The questionnaire specifically asked whether farmers passed on any of the agronomic recommendations, e.g., close legume spacing, in addition to distributing seed. In this way the FTT approach monitors and evaluates changes to five of the boxes shown in Figure 3, that is, changes in farmer knowledge and perceptions; modifications; adoption; and the spread from the pilot villages elsewhere (scaling-out). Table 10 shows that over half of the farmers had made at least one modification to researcherrecommended management when they adopted aspects of ISC. Most of these were to reject the researcher-recommended sole-cropping and closer plant spacing, in particular for soybean. The latter was largely because recommended soybean row spacing was 35cm, while in most farmers' fields row spacing was fixed by the local animal-driven plough at 70cm. One farmer, however, came up with the innovative approach of planting two rows per ridge, shown in . It shows that the farmer has understood the principle of suicidal germination and the need for higher soybean root density. The project has subsequently adopted this practice because it reduces the cost of establishing legume trap crops. Farmers' rejection of sole cropping has negative implications for Striga control because mixed cropping with cereals (gicci, strip, intercropping and relay) is a concern to the project because it means that Striga will grow and flower each year in the field thus replenishing the seed bank. The reasons farmers gave for continuing with mixed cropping is that it reduces risk, and gives a higher overall yield. Both reasons are valid, although the second is only true with low fertilizer use that necessitates wide cereal plant spacing, leaving room for a legume in-between. However, researcher-managed trials have shown that a sole-crop legume followed in rotation with close-spaced sole-crop maize with moderate fertiliser application rates gives better Striga control and much higher yields than farmers' practice, and is more profitable (Schulz et al. In press). In an effort to bring farmers' and researchers' perceptions and understanding closer together the project is planning to carry out a participatory budgeting exercise at the end of the 2002cropping season to help farmers more clearly see the economic benefits of sole-cropping and legumecereal rotations, and for researchers to better understand the benefits of mixed cropping. This would not have happened without the data from the monitoring and evaluation. In Kaya village, for example, adoption is clustered around the participating farmers and their fields, while in Mahuta, aspects of ISC have moved up to 40km. The project is now planning to develop a ISC extension approach built on fostering these indigenous scaling-out mechanisms. Again, without M&E the project would not be following this path. M&E has helped give the ISC project a much clearer impact focus through the process of defining the project's impact pathway. The M&E findings have redirected research efforts in a number of ways, including through the incorporation of farmer innovations in the recommended basket of options; the decision to carry out a partial budgeting exercise to bring farmers' and researchers' perceptions of the pros and cons of mixed versus sole cropping closer together; and by providing the understanding of adoption processes necessary to develop an effective ISC dissemination approach.In 1998, the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) and its partners in Malawi and Zimbabwe began a comparison of different Farmer Participatory Research (FPR) approaches being used by various organizations in Malawi and Zimbabwe. A key objective was to compare the effectiveness of different methodologies in the development and testing of soil management technologies for resource-poor farmers, particularly women farmers. A second objective was to investigate the contribution that crop systems simulation modelling could make to FPR. To accomplish the objectives the project tested FPR methods at each of six case study sites, three each in Malawi and Zimbabwe. The various methods involved varying degrees of farmer participation: traditional researchled, researcher-led with farmer input, and farmer-led with research input.The project tested four hypotheses:• The provision of a broad range of soil water and nutrient management options is better than blanket fertiliser recommendations currently offered by government extension services, which are 3 to 8 times higher than the rates that farmers usually apply; • Recommending less inorganic fertilizer and more manure and legumes to women farmers would increase their adoption rates, and thus improve productivity and food security.• Uptake of technologies would vary with the wealth of the household and gender of the household head.• Adoption would follow a cycle starting with a low rate of inorganic fertilizers and manure and increase through learning-by-doing and learning-by-using.At start-up the project invested heavily in training activities with research and development staff from the different partner institutions to introduce the various concepts of the participatory research process and simulation modelling. However, the short duration of the project meant that much of the problem identification and selection of best bets occurred prior to the training. The 'best bet' technology options were selected in part through an exercise in systems analysis conducted in September, 1999, based on crop simulation modelling and resource-constrained scenarios that incorporated realistic levels of farmer resources. Socio-economic data and the judgement of agronomists and economists were used to devise resource-constrained scenarios (e.g., trade-offs for allocation of labour and resources among different farm management components: timely planting and weeding, fertilizer and manure). Simulation was then used to evaluate different scenarios, with the goal of maximizing return from the whole farm while limiting risk. The best bet options chosen included a range of legume intensified systems (Chamango, 2002;Twomlow et al., 2002), moderate inorganic fertilizer use alone or in combination with extra weeding (Dimes et al., 2002), and manure (Murwira and Kudya, In press).Throughout the projects life various formal and informal workshops were held in both Malawi and Zimbabwe to introduce various concepts of the participatory research process and simulation modelling to researchers, extensionists and farmers. On-farm experimentation with the best bet options was then conducted over the 1999/2000 and 2000/2001 growing season at the six case study sites. Each case study village had a research-led, farmer-input trial and either a farmer-led, research-input and/or a research-led, traditional trial. The choice and implementation of the best bet options and the FPR approaches varied depending on the experiences of the local team. To fully understand the household labour implications and farmer perceptions of the different technologies being tested at the six field sites field days were held on a seasonal basis and farmers perceptions of each technology were solicited using a range of participatory tools, such as matrix ranking. These field days were followed up by a series of focus group discussions between March and May 2001, with participating farmer groups in both countries (Rusike and Twomlow, unpublished field notes; Ncube and Twomlow, 2001). At each of these meetings the farmers were asked to describe their cropping calendar in relation to local soil taxonomies, the labour resources used for each task, and local input and output prices, the technologies they had been evaluating, and what, if anything they had adopted or adapted (see ). This information was then used to construct a final adoption survey from which partial budgets for each technology were determined, from which net benefit curves and a marginal rates of return analyses were calculated to identify what financial benefits might accrue for a rural household and the potential risks of the different technologies. Based on this information a whole farm mathematical programming model was constructed to determine profitability of the improved soil water and fertility management options relative to other investment options given the household resource constraints and preferences for risk, and thus identify constraints to adoption. The model was set up to maximize net revenue of the whole farm subject to resource constraints. The model captured risk as the minimum quantity of staple food grain (1 ton of maize, sorghum or millet grain per 6 family members) that the household needs to produce to meet its food requirements for the year. The model was then run for different resource categories of farmers using the gender of household head as the proxy indicator. The FPR trials indicated significant yield increases result from use of best-bet options compared to farmers' practice, including small quantities of fertilizers linked to weeding, legume rotations and intercrops and anaerobically-composted manure.Marginal rate of returns analysis showed that best bet technologies offered significant benefits to farmers and had returns which exceeded 100%, which is usually assumed to be the minimum required for smallholders to widely adopt this type of agricultural technology (see Table 11 and Table 12 for example marginal rate of return analyses for legume best bet trials in Malawi and Nitrogen by Weeding Trials in Zimbabwe). However, farmers' evaluation of technologies, using matrix ranking exercise during field days, showed that they may still not adopt the technologies despite their competitive marginal rates of returns shown in Table 11 because of resource constraints, access to input and output markets, risk and food security (Table 14). Nevertheless, the 2000/2001 end-of-season survey of farmers who hosted trials and non-host farmers in neighbouring villages showed that farmers are adapting and adopting some technologies from the trial plots to their main fields (Table 14). The crop management practices being adopted/adapted by farmers in both Malawi and Zimbabwe are summarised in Table 11. The adoption of maize-legume systems by households in both countries is influenced by access to input markets for seed and output markets to earn cash through commercial grain sale. In both countries the use of inorganic fertilizer is constrained by a combination of high fertilizer prices, and blanket recommendations that do not take account of households perceptions of risk and liquidity, as few households earn enough income from their crop sales to enable them to invest such large cash inputs. The small doses of fertilizer, 10 to 20 kg of N ha -1 that the project tested with farmers appears to be at an investment level that households are willing and able to risk. However, information is lacking on fertilizer application rates that farmers currently use, how they can best use the small quantities of fertilizer they have, and on manure-fertilizer combinations, and integrating inorganic and organic fertility amendments.Table 15 shows that in both Malawi and Zimbabwe the most popular technologies were improved germplasm with accompanying management practices. However, the table also shows that farmers in Zimbabwe were much more willing to expand investments in a range of improved soil, water and nutrient management practices than in Malawi, where pre-plant ridges are the norm, rather than the exception.Other findings from the research showed, however, that adoption of soil, water and nutrient management practices should be accompanied by improvements in basic crop management practices such as variety selection, tillage, planting method, spacing, timing of planting and weeding in order for the investment in improved soil fertility management to provide acceptable payoffs.In Malawi, it would appear from our survey data that proportionately more female-headed households have changed their crop management practices as a result of the projects activities. Female-headed households are adopting new maize varieties; groundnut, soybeans and Tephrosia intercrops; and modifying their plant populations to reflect those used in the trials (Table 10). In contrast, male-headed households were emphasising pigeon pea and Mucuna rotations, incorporation of plant residues, kraal and compost manures, and early planting and small quantities of fertilizer. These difference have been attributed to the fact that female-headed households tend to have greater land, labour and cash constraints, and as a consequence are more food deficit than male-headed households (Freeman, 2002). , 2001) showed that farmers' perceptions of soil fertility management vary widely, depending on the resources available to the individual household. In Zimbabwe, de facto female-headed households with access to cash appeared to be adopting new cereal varieties, seed priming, pit-composted manure and small quantities of inorganic fertilizer, manure and weeding combinations (Table 11). In contrast, the poorer resourced de jure female-headed households were adopting heap-covered composted manure, dead level contours, modified-tied ridges and reduced tillage because they have more severe capital and labour constraints. Male-headed households with access to labour and draught animals but not cash favoured legume rotations, small doses of fertilizer and water harvesting, especially infiltration pits that are labour-intensive. Solutions from the whole farm mathematical programming model suggest that for male, de facto and de jure headed households that the most attractive technologies are: Legume-rotations and treated manures for male-headed households with access to draft animals, labour and land; Small quantities of inorganic fertilizer, treated manure and manure-fertilizer technologies for de facto female-head households with an off-farm cash income; Legume intercrops in the female-headed households, typically de jure, who have the most severe resource constraints.The model outputs disagreed with the working hypotheses based on expert opinion, and show that the wealthiest households, irrespective of gender, with lowest opportunity cost of working capital, will allocate more land and money to inorganic fertilizers than the more marginalised groups. These marginalised groups typically have less working capital and a higher opportunity cost. Therefore, betterresourced households will invest in high input technologies such as small quantities of fertilizer and treated manure. In contrast, the poorer resourced households will invest in low input technologies such as legume-cereal intercrops, as their opportunity cost of labour is very high and they are better off selling labour to wealthier households.Overall, three major lessons have been learned about the differential adoption and targeting of alternative soil water and nutrient management to differently resourced households: 1. Small quantities of fertilizer and manure-fertilizer combinations have a high payoff and supplying inorganic fertilizers in small packs reduces the liquidity constraint and enhances returns to investment in chemical fertilizers. 2. Input and output markets drive legume intensification. 3. Legume intensification needs to target poor households for food for home consumption and wealthier households for cash income through producing marketable surpluses.Another conclusion is that a pure farmer-led approach may not always be appropriate where researchers have spent time understanding the farming system and the externalities that impact upon it, simulation playing an important part in this understanding. A major advantage of linking FPR and simulation modeling was the co-learning that took place between the researchers and the farmers about the impacts of climatic risk and resource endowments, and how they influence household's investment choices. In fact, if Integrated Natural Resource Management (INRM) research is about better communication and more effective interaction on the part of researchers with managers (about system management), then linking simulation modeling and participatory research should be viewed as an integral part of applied INRM in smallholder farming systems. The results also indicated that when there are no clear procedures to directly target women farmers, and gender issues are not sufficiently integrated into the research process they tend to be under-represented.The case studies show clearly that rural technology change, brought about by the generation and diffusion of new technologies, is an evolutionary and highly complex process. An evolutionary process is one in which novelties are generated, selections of beneficial novelties are made and these improvements are retained and promulgated (Douthwaite et al. 2002). The Uganda and Nigeria case studies show that farmers were actively modifying the technologies in ways that improved their 'fitness' or adoptability. In Uganda farmers sought alternative uses for the LCC and BT technologies that would increase the return on their investment in labour and land. In Nigeria, farmers sought to find compromises between the 'best bet' agronomic practice and what fitted their own systems. Some of these innovations have been incorporated in the recommended package for Integrated Striga Control. The Zimbabwe and Malawi case study showed how important these compromises are because farmers do not select technologies purely on agronomic or economic performance, which is often the basis by which researchers select their 'best-bets' for their trials (see Figure 1). Instead farmers chose to adopt based on a number of factors that is dependent on the resources available to that household, perceptions of risk and the gender of the household head. The Uganda case study showed that preferences for technologies changes from location to location. Hence, identifying farmer adaptations through M&E is an important source of incremental improvements to a technology and future research areas, as well as providing good insights into farmers' perceptions and motivations.The case studies show that M&E also has a crucial role to play in identifying differences in perceptions between researchers and farmers, and between individual farmers in their responses to new ideas and technologies. In all three case studies it was impossible for researchers to know beforehand how different farmers would react to the new technologies. However, the M&E carried out gave this information and has allowed the projects to adjust accordingly, thus making widespread adoption and impact more likely. For example, the M&E exercise helped ICRISAT and its partners to see that de jure women-headed households can generally only adopt the lowest input technology, which was the legumecereal intercrops because they did not have the cash to buy inorganic fertilizer, or the labour to make and spread compost. In Nigeria, M&E findings showed that many farmers were adopting improved germplasm but not the concepts of sole cropping and crop rotation, preferring instead to continue with their own mixed cropping practices. In response the project will carry out participatory partial budgeting to examine together with farmer the advantages and disadvantages of sole versus mixed cropping.The three case studies largely confirm the conceptual map of the development and adoption process adopted in the case studies shown in Figure 1. All the case studies found that there is a role for researchers to take the lead in introducing 'best bets'. Those 'best-bets' are more likely to adopted and adapted by farmers when researchers have spent time understanding local farming systems, and, in the case of Zimbabwe and Malawi used simulation modelling to evaluate different options with farmers. Purely farmer-led participatory technology development is less likely to bring new ideas into to community because researchers have a deeper knowledge and understanding of new technical options, while farmers are concerned with their current problems and have little human and financial resources to search for and adapt novel solutions. However, whatever the source of innovation, once adoption begins, M&E has a role to play in facilitating the close interaction between farmers and researchers required to co-develop new technologies to make them more widely adoptable.The Zimbabwe/Malawi and Nigerian case studies looked at farmer to farmer spread of new technologies and ideas, in other words the selection and promulgation functions of the evolutionary innovation process. Whether farmers recommend a new technology to others, and who they recommend to, are the most important indicators of whether a technology is likely to scale-out, where it will go, and how fast. The Nigerian case study is using a GIS-based approach to map the adoption/adaptation, selection and promulgation process and is developing an extension approach that complements existing promulgation channels.Thomas Kuby has developed an impact model, shown in Figure 8, based on experience at GTZ (Kuby, 2000). The model shows projects carrying out their own M&E, similar to ways in which M&E has been carried out in the three case studies, to the point of assessing whether the project has delivered intended benefits, or unintended benefits and negative consequences. Assigning this role to the project comes from years of project experience that has shown that: \"as a rule, self-evaluation is more critical and better value for money than external monitoring -and that it makes a much greater contribution to learning, both in the projects and in the whole organisation\" 6 (Kuby, 2000 p. 4). This learning helps make impact more likely, as well as contributing to priority setting in the organisation.Formal and static routines are not adequate for research priority setting due to the essential unpredictability of complex systems. Since many outcomes cannot be forecast, expected impacts cannot guide priority setting. Even though particular outcomes cannot be predicted, it is possible to identify factors that will, with high probability, affect the chances of success or failure. More flexible approaches (such as adaptive management) require strong M&E to identify as early as possible unintended (both positive and negative) consequences so that appropriate responses can be implemented. M&E also has a role to play to ensure that all stakeholders understand the processes that generated the outcomes.In addition to contributing to organisational learning, M&E can help all partners, from farmers to donors, to learn from the research to adoption process. The three case studies presented here showed that farmer adoption and subsequent innovation and adaptation are invaluable indicators of the likely adoptability of the introduced options. Early identification of farmer adoption / non adoption and modification allows the research process to be adapted and allows the setting of new priority areas for research. Without this flexibility in the approach, 'best bet' options demonstrated on-farm are unlikely to undergo the necessary co-development necessary to be more widely adopted.The Kuby model shows an attribution gap between a project's direct benefits and wider, more highly aggregated impacts, for example poverty alleviation, that might result from these benefits. Hence the model agrees with our earlier discussion of why attribution of impact is nearly always impossible because of the interconnected nature of causes and effects.The causes and effects that lead from a project's direct benefits to broader impacts result from two linked processes that are known as scaling-out and scaling-up. Scaling-out is a horizontal spread of an innovation from farmer to farmer, community to community, within the same stakeholder groups. Scaling-up is an institutional expansion from grassroots organizations to policy makers, donors, development institutions, and other stakeholders key to building an enabling environment for change. Both are linked because as a change spreads further geographically the greater the chances of influencing those at higher levels, and likewise, as one goes to higher institutional levels then the greater the chances for horizontal spread.The Nigerian case study shows how M&E based on an impact pathway can form the foundation of a plausible ex post impact assessment by making explicit the source of impact (the project's direct outputs), the impact model, the impact pathway and the impact hypotheses. The impact pathway evolves during the duration of the project as M&E identifies incipient scaling out and up processes including processes of knowledge generation and diffusion, the emergence and evolution of innovation networks, and the creation of organisational capabilities. Hence, the description of the impact pathway at the end of the project would be an invaluable starting point to ex-post impact assessment some years after. Indeed, without process M&E, plausible ex-post IA of INRM projects, based as it needs to be on a convincing explanation of process, will be extremely difficult.The M&E approaches described in the three case studies are relatively new and are not yet well institutionalised in their respective CGIAR Centres. Whether they are or not will depend on three factors:• Being able to demonstrate to fellow researchers that the benefits of M&E are worth the cost;• Having the capacity to carry out effective M&E;• Support for M&E from senior management.Of course, all three are linked. If CGIAR scientists can come to see M&E as something useful and not threatening then support from senior management is likely to follow, together with additional capacity to carry out the work. Experience shows that self-monitoring is less threatening and more useful than external M&E, which suggests that individual projects within Centres should be responsible for M&E, but with backstopping from an M&E unit.For ages, agricultural production depended on organic resources for soil fertility replenishment, either by including long-term fallow periods, as was, e.g., the case in sub-Saharan Africa (SSA), or by application of vast amounts of manures or other organic resources, e.g. sods of peat in northern Belgium (Dudal, 2001). The use of fertilizers started in western Europe only at the end of the 19 th century in response to a higher demand for food. Other continents followed at a later stage, but even up to the mid-1960s, fertilizer use in SSA was restricted to export crops such as groundnut, cotton, coffee, tobacco, or oil palm (Dudal, 2001). During the 'Green Revolution' in the 1960s in Asia and Latin America organic resources were not considered essential in boosting agricultural production. In this context, Sanchez (1976) stated that when mechanization is feasible and fertilizers are available at reasonable cost, there is no reason to consider the maintenance of soil organic matter (SOM) as a major management goal. However, application of the 'Green Revolution' strategy in SSA resulted only in minor achievements because of a variety of reasons (IITA, 1992). This, together with environmental degradation resulting from the massive applications of fertilizers and pesticides and the abolition of the fertilizer subsidies in SSA, imposed by structural adjustment programs led to a renewed interest in organic resources in the early 1980s (Table 1). This interest has only grown stronger in recent years driven by the development of an Integrated Soil Fertility Management (ISFM) strategy for soil fertility replenishment of which the combined application of organic resources and mineral inputs forms the technical backbone. In this context, Sanchez (1994) revised his earlier statement by formulating the Second Paradigm for tropical soil fertility research: 'Rely more on biological processes by adapting germplasm to adverse soil conditions, enhancing soil biological activity and optimizing nutrient cycling to minimize external inputs and maximize the efficiency of their use'.Since the early 1980s, progress in developing organic resource management related knowledge has been substantial, driven by the hypotheses formulated by Swift et al. (1979) and Swift (1984Swift ( , 1985Swift ( , 1986)), culminating in an International Symposium in 1995 (Table 1). As a result of the Symposium, efforts were made to consolidate information on residue quality -N dynamics relationships resulting in an Organic Resource Database (ORD). The ORD contains information on organic resource quality parameters and N mineralization dynamics from almost 300 species found in tropical agroecosystems (Palm et al., 2001). A careful analysis of the information in the ORD has led to the development of a Decision Support System (DSS) for organic matter (OM) management (Fig. 1) (Palm et al., 2001). The DSS makes recommendations for appropriate use of organic materials, based on their N, polyphenol, and lignin contents resulting in four classes of organic resources (Palm et al., 2001). For instance, high quality organic resources with a N content > 2.5%, a lignin content of < 15% and a polyphenol content of < 4% are recommended to be applied directly to the soil as these are expected to release a substantial part of their N in the short term (Fig. 1). Medium quality organic residues having < 2.5% N and < 15% lignin, or > 2.5%N and a polyphenol content > 4%, on the other hand, are recommended to be applied together with fertilizer N or high quality organic resources. Lastly, low quality organic resources with a low N and high lignin content are recommended to be surface applied as such residues would result in the most substantial mulch effects. The combined application of organic resources and mineral N is hypothesized to yield added benefits in terms of extra yield or improved soil fertility compared with the sum of the responses in the treatments with sole application of organic resources and mineral N. A Direct and Indirect Hypothesis which could form the basis for the occurrence of such benefits has been formulated by Vanlauwe et al. (2001). The Direct Hypothesis was formulated as: Temporary immobilization of applied fertilizer N may improve the synchrony between the supply of and demand for N and reduce losses to the environment. The Indirect Hypothesis was formulated for N supplied as fertilizer as: Any organic matter-related improvement in soil conditions affecting plant growth (except N) may lead to better plant growth and consequently enhanced efficiency of the applied N. Both hypotheses, when proven, lead to an enhancement in N use efficiency, processes following the Direct Hypothesis through improvement of the N supply and processes following the Indirect Hypothesis through an increase in the demand for N. Obviously, mechanisms supporting both hypotheses may occur simultaneously. The objectives of the current paper are (i) to validate the concepts proposed in the DSS with field data, including plant materials and animal manure as organic resources; (ii) to explore the occurrence of added benefits when applying organic resources in combination with mineral N, and (iii) to reflect on the activities required to develop the DSS into a practical recommendation tool.A greenhouse trial was carried out in Ibadan, southwestern Nigeria, aiming at quantifying immediate and residual relationships between organic resource quality and maize N uptake (Vanlauwe et al., unpublished data). A range of organic materials containing between 0.14 and 3.53% N was applied in pots with a Nitisol from Southern Benin Republic at an equivalent rate of 90 kg N ha -1 and maize was grown for 7 weeks. After harvesting the first crop, a second crop was grown for another 7 weeks without fresh residue application. Total N uptake by the maize in the shoots and roots was measured at each harvest. In East and southern Africa, a set of field experiments was set up to determine the fertilizer equivalency values of organic resources (Murwira et al., 2001). Each trial contained a set of locally available sources of plant materials or cattle manure. The organic resources were applied on the field in a randomised complete block design which included a number of plots aimed at determining the response to fertilizer N using maize as a test crop. Based on the response curve and the yield increases in the organic resource treatments, fertilizer equivalency values were calculated and converted to percentage fertilizer equivalency values (%FE) taking into account the N application rates of the organic materials.In West Africa, a multilocational set of field experiments also using maize as a test crop was established using various inputs of plant materials -and cattle manure in a single case -and the %FE was calculated using the similar approach as indicated above (Vanlauwe et al., 2002). In both sets of trials, P and K were applied in non-limiting quantities to ensure that N was the sole nutrient limiting maize production.Several trials were established in the various sub-regions aiming at quantifying potential added benefits in treatments with combined applications of organic resources and mineral N (Table 2). All cropping systems considered were organic resource transfer or biomass transfer systems using maize as a test crop. Added benefits were mathematically evaluated using the equation:where AB signifies Added Benefits and Y con , Y fert , Y OM , and Y comb mean grain yields in the control treatment, in the treatments with sole application of fertilizer and organic matter, and in the treatment receiving both inputs, respectively (Vanlauwe et al., 2001). In equation 1, the yields are adjusted for similar amounts of organic resources and mineral N applied in the combined as in the sole treatments, following information obtained through the N response curve or if the latter is absent assuming linear responses to applied organic and mineral N.The greenhouse trial data clearly show a significant positive relationship between the organic resource N content and the total maize N uptake of the first crop (Fig. 2). Low quality materials such as maize stover or sawdust immobilized N resulting in less N uptake compared to the unamended control. For the second crop, however, the relationship was negative, indicating that the medium to low quality materials provide more N to a second growing maize crop compared to the high quality materials (Fig. 2). Even in the treatment with maize stover, no further immobilization of N was observed. Only the sawdust treatment kept the N immobilized beyond the second crop. These data show that while organic resources with a high amount of available N can immediately stimulate crop growth, while for medium to low quality materials, residual N supplies are greater. More cropping cycles would be needed to judge whether the cumulative yields are similar for the high and low N organic resources. Cadisch et al. (1998), on the other hand, observed no compensation in initial N release from low quality, high polyphenol containing prunings at later harvests compared to high quality materials and attributed this to the stability of polyphenol-N complexes. The data also indicate that for materials with a N content below 1%, additional N should be applied either as fertilizer or as high quality organic matter to overcome the negative impacts caused by N immobilization. Data from the field experiments in West, East and southern Africa show that the percentage fertilizer equivalencies (%FE) values for organic materials with a low polyphenol content (< 4%) and a N content > 2.3% were positively related to their N content (Fig. 3). The critical level of N for increasing crop yield was 2.3%, confirming the initial value hypothesized by Palm et al. (2001). Organic matter with a high polyphenol content (> 4%) still led to positive %FE values, but the increase with increased N content was less and the N content needed to improve maize yield was 2.8 rather than 2.3% (Fig. 3). Polyphenol -N interactions seem to delay the immediate availability of N as concluded by others from data obtained under controlled laboratory or greenhouse conditions (Palm andSanchez, 1991, Oglesby andFownes, 1992). Data obtained with Calliandra calothyrsus residues did not show a consistent trend. While in all cases their polyphenol content was high, data from certain sites did not show any reduction in %FE. This may be related to the specific rainfall patterns, as high rainfall immediately after applying the Calliandra residues may remove a substantial part of the polyphenols through leaching. While from the current data polyphenols appeared to be under certain conditions important modifiers guiding initial N release from organic materials, the lignin content was not observed to improve on the derived equations. This does, however, not exclude their importance in medium to long term N dynamics, as shown in the greenhouse experiment (Fig. 1) and discussed below.Some organic resources led to N fertilizer equivalency values exceeding 100%, especially in the case of Tithonia diversifolia (Fig. 3). This is likely caused by a better synchrony between the supply of and demand for N derived from Tithonia residues than for immediately available fertilizer-derived N. Mineral N inputs are readily available and as such prone to leaching and/or gaseous losses, even if split applied.Manure does not show a consistent trend across sites (Fig. 3). Very low N containing cattle manure was observed to decrease crop yield but fertilizer equivalency values of manure containing between 0.7 and 2.4% N were almost similar and equal to about 35%. N content alone could not satisfactorily explain the observed responses to manure application indicating that other indicators are necessary for quantitative evaluation of manure. This may be related to changes in quality and partial stabilization of the organic resources while passing through the rumen or while storing pending application on the field. Nzuma and Murwira (2000) showed considerable differences in manure quality when stored in a pit or heap. Manure may require other indicators for assessing its quality, likely based on nutrient and biochemical components the soluble fraction rather than on the overall material.Organic resources with a N content below 2.5% would need to be applied in combination with additional mineral N to substantially increase crop yields (Fig. 3). Significant added benefits in treatments with combined application of organic resources and mineral N do occur in various experiments although the mechanisms governing these benefits are not always clearly understood. In the experiment in Zimbabwe with various mixtures of cattle manure and ammonium nitrate, added benefits ranging between 663 and 1188 kg maize grains ha -1 were observed by Nhamo (2001), as calculated using equation 1 (Fig. 4). The author related this to the supply of cations, contained in the manure, which may have alleviated constraints to crop growth caused by the low cation content (CEC varied between 1.2 and 2.5 cmol c kg -1 with an average of 1.7 cmol c kg -1 ) of the very sandy sites (clay content varied between 2 and 10% with an average of 4%). Although temporary immobilization of fertilizer N by decomposing manure can not be excluded, this may be less likely as the C/N content of the used manure was below 10, assuming that this would be a suitable indicator for assessing N dynamics of manure. In a trial in central Kenya, Okalebo et al. (2002) similary observed added benefits of 684 kg grains ha -1 in 1998 when mixing low quality wheat straw and soybean trash with urea for an acidic Ferralsol (pH-water of 4.9) (Fig. 5). After application of the organic residues, the pH-water increased to 5.4, on average, while pH in the control soils remained unchanged. Rainfall in 1997 was low and not well distributed leading to absence of major responses to applied N. Mucheru et al. (2002) observed added benefits ranging from -250 to + 550 kg maize grains ha -1 during the short rainy season of 2000 (Fig. 6). Values for the long rainy season, which experienced lack of rainfall after germination, were not different from 0. These benefits varied substantially for the different organic resources used. The high amount of K in the Tithonia residues may have caused the substantial added benefits in the combined Tithonia-N fertilizer treatment, as earlier observed by Sanchez and Jama (2001). Besides supplying K, Tithonia residues have been shown to ameliorate soil aggregation, reduce P sorption sites, reduce P-metal complexes and Al-toxicity (Cong, 2000). Causes for the added benefits created in the cattle manure treatment are not clear.While in the above experiments, added benefits were observed only for certain organic resources, in the Sekou experiment, in which organic resources with a N content varying between 2.4 and 4.7% were used, similar added benefits were observed for all organic resources (Fig. 7). As the site experienced drought stress during maize grain filling, Vanlauwe et al. (2002) attributed the added benefits to improved soil water conditions in the mixed treatments, caused by the surface or sub-surface placement of the organic resources, compared to the treatment with sole application of fertilizer. Alleviation of moisture stress may have improved the N use efficiency of the applied fertilizer. In formerly discussed trials with organic resources, no alleviation of moisture stress was observed (Figs. 5 and 6) but in these trials, organic resources were incorporated. During seasons with shortage of rain, this residue management practice has been shown not to substantially alter soil moisture conditions vis-à-vis surface or subsurface placement (Minhas andGill, 1985, Sembiring et al., 1995). No soil water data were taken, so the above would need to be evidenced.The final product of all above work should be a tool to assist farmers on how to optimally manage their scarcely available organic resources and costly mineral fertilizers, preferably adapted to their biophysical environment and targeted yields for specific crops. Although this seems like an impossible task, generation of the following information would signify substantial progress: (i) generation of a more detailed understanding of the mechanisms creating added benefits, (ii) assessment of the influence of intrinsic soil properties (e.g., texture and clay mineralogy, water holding capacity), and climate conditions (e.g., risk of rain shortage) on the latter, (iii) quantification of the residual effects of organic resources of varying quality, applied sole or in combination with fertilizer, on crop yield, and (iv) evaluation of the immediate and residual responses of other crops besides maize to applied organic resources and fertilizer.The mechanistic basis for added benefits created through positive interactions between OM and mineral N is broad and has not been clearly understood yet. Although in the above case studies, likely reasons behind the added benefits could be put forward, little or no evidence was gathered to substantiate these. Trials which explicitly quantify some of the changes in soil properties as affected by OM application are needed. Such trials would also include treatments in which the hypothesized constraint to crop growth is alleviated using external inputs containing only the agent addressing this constraint. Although it may be an illusion to aim at understanding all interactions between organic resources and fertilizer under all conditions, under certain specific conditions, clear organic resource-related improvements in soil fertility status could be identified. For instance, the use of high P containing manure or compost on low P soil could lead to an improved use efficiency of N fertilizer and consequently added benefits. When looking at legume-cereal rotations, the mechanisms potentially creating added benefits may even be more diverse relative to the ones discussed in biomass transfer systems in this paper. Many rotational effects are often explained in terms of changes in pest and disease spectra during legume growth (Akhtar, 2000) or in terms of legume rhizosphere processes (Vanlauwe et al., 2000).Organic resources are known to show some residual effects. Optimal nutrient management strategies need to take into account these effects. In the long term, an improved soil organic matter status may equally lead to enhance N fertilizer use efficiencies, although quantifying the latter may prove very difficult. Vanlauwe et al. (unpublished data), e.g., showed a negative relationship between the proportion of maize N derived from urea and the soil total N content, presumably caused by a higher supply of native soil N in soils with a higher total N content.Most of the data presented in this paper, and similarly in other work dealing with soil fertility management, were obtained in maize-based cropping systems. It is likely, however, that farmers would prefer to use their often scarcely available organic resources on crops which yield more income. In this context, it has been observed that farmers in western Kenya would rather apply high quality Tithonia residues to kale (Brassica oleracea) rather than maize (Jama, personal communication). This would not necessitate to initiate a vast amount of trials using other crops than maize, but to consider the nutrient uptake patterns of those other crops and use the information obtained for maize to test specific hypotheses related to the potential effects of organic resources and fertilizer on other crops.After having obtained relevant information as described above, two extra steps may be required to complete the development of a user-friendly decision aid: (i) all above information needs to be synthesized in a quantitative framework and (ii) that framework needs to be translated in a format accessible to the end-users. The quantitative framework could look as presented in Fig. 8 for a situation where all interactions between organic matter and fertilizer happen through N immobilization reactions, thus supporting the Direct Hypothesis. Temporary immobilization of fertilizer N by medium to low quality resources may reduce the potential for losses of fertilizer N materials and consequently less fertilizer N may be required to reach the same amount of available N (the 'added benefits' situation in Fig. 8). If the immobilization lasts beyond a growing season, on the other hand, additional fertilizer N may be required to reach the same amount of available N (the 'immobilization' situation in Fig. 8). The current concept may be adapted to initial soil fertility status by including a background soil N supply and to different crops. In case the mechanisms creating added benefits following the Indirect Hypothesis are known, the concept could also be adapted to these conditions. The final format of the decision aid should take into account the realities on the field. Some of these realities, among others, are: (i) large scale soil analyses are not feasible, so local soil quality indicators need to be included in decision aids as farmers use those to appreciate existing soil fertility gradients within a farm; (ii) conditions within farms vary as does the availability of organic resources and fertilizer, therefore rules of thumb rather than detailed quantitative recommendations would be more useful to convey the message to farmers; (iii) farmers decision making processes involve more than just soil and crop management; and (iv) access to computers, software and even electricity is limited necessitating hard copy-based products.Although the data obtained largely support the concepts outlined in the DSS for organic N management, the reality on the field is such that the availability of high quality, fertilizer-like organic materials is very limited. Therefore, the arms dealing with medium to low quality organic resources are likely to be most relevant for real cropping systems. Such organic resources are recommended to be applied in combination with mineral fertilizer, and when doing so, added benefits do occur, although their mechanistic basis if most of the time not clearly understood. The relevance of such potential added benefits needs to be assessed for various biophysical environments and crops. In conditions where it is difficult to assess these potential benefits, assuming additive effects is usually good enough as a first approach as negative interactions are not commonly observed. Finally, generation of the needed knowledge will by itself not change the way farmers are managing their organic and mineral resources. This knowledge needs to be condensed in tools adapted to the clients targeted.Vanlauwe B., Aihou K., Aman S., Iwuafor E.N.O., Tossah B.K., Diels J., Sanginga N., Lyasse O., Merckx R. and Deckers J., Maize yield as affected by organic inputs and urea in the West-African moist savanna, Agronomy Journal (2002) In press. Much work has been done on understanding the effects of manure on crop response, and on manure quality and how quality can be improved by better methods of composting and beneficiation with inorganic fertilizers especially rock P (Mugwira and Murwira, 1997). Several workers have reported the beneficial effects of combining manure with inorganic fertilizers (Murwira, 1993;Mubonderi, 1999;Nhamo et. al., 2001, Munguri ,1996 ). Use of combinations can help synchronise nutrient supply and crop uptake, improve on-farm nutrient cycling, reduce environmental pollution (N and P) and be used to manage mineralisation-immobilisation processes. Placement studies have also shown that broadcasting manure was less effective than banding and station placement (Munguri, 1996).Recommendations on rates of manure application for field crops are varied but difficult to compare across sites because nutrient content data is often not cited (Mugwira and Murwira, 1997). It is however difficult to come up with prescriptive guidelines on use of manure as the quality varies considerably from farmer to farmer because of they way it is managed and stored prior to application in the fields (Murwira et al, 2001). Our challenge is to translate the scientific understanding we have into farm practice taking into consideration quality and quantity of manure available, short-and long-term effects, economic factors, environmental factors, farmer perceptions and limiting nutrients. This requires sharing with farmers the scientific principles of using manure and the development of communication strategies that could bring about a positive change in the way farmers manage resources available to them. Understanding farmer decision making is a key to this process and we advocated in this study an approach that entails the development and use of decision guides to bridge the gap between researchers and farmers.It has been argued that farmers make decisions on fertilizer and organic resource use in a decomposed fashion using relative comparisons of singular alternatives rather than holistic assessments of utility (Schoemaker, 1982). In other words the decision to use a specific type of input, its utility, is based on asking oneself a series of questions that lead a choice. The decision making process can thus be described as a decision tree which is a sequence of discreet decision criteria, all of which have to be passed along a path to a particular outcome or choice (Gladwin, 1989). The biggest assumption behind the use of decision guides is that the decision-makers themselves are the experts on how they make the decisions they take. Therefore it is crucial to elicit from the decision-makers themselves their decision criteria which can then be presented in the form of a decision tree.Early decision trees attempted to use socio-economic variables such as labour availability, cash income, livestock ownership etc to arrive at 'recommended actions' for different socio-economic scenarios. Using a reductionist logic based on binary oppositions (e.g. livestock owners Vs non-livestock owners, female headed Vs male headed households, labour-rich Vs labor-poor farmers etc), it was assumed that an analyst could determine the series of decisions which farmers should make when choosing between technological options. The main criticism is that such decision trees can never sufficiently mimic the much more diverse and dynamic realities under which different individual farmers operate. For example, it is not always possible to conclude that a livestock owner should behave differently from a non-livestock owner because the latter may use a number of local arrangements and networks to gain access to cattle or cattle resources. Similarly a 'resource poor' farmer may suddenly find himself or herself with sufficient inorganic fertilisers accruing from different possible sources. In such situations, individuals do not stop and say, 'since I am classified as a resource-poor farmer in a decision tree, I will manage my fertiliser in this way'. The bottom-line is that the binary permutations constructed by analysts in the form of decision trees cannot capture the flow and flux of everyday life, which determine farmer decision making and choices. Also it is necessary to make a distinction between describing what farmers do and offering them options e.g. what can you do if you have livestock or if you have livestock you can do the following.Other types of decision trees have binary categories that are constructed on the basis of relatively stable bio-physical variables such as N content, lignin content etc (Palm et. al, 1997). These variables are not amenable to direct manipulation by individual farmers, nor are they subject to sudden socio-economic upheavals such as credit availability, access to markets and so on. Having said so, it is important to recognize that farmers' decisions are based on a judgement between options that relate to the whole range of economic, cultural and biological parameters (Swift et. al, 1994). Categories are also less fixed than may be apparent (Palm et. al., 2001) because of modifiers which for example, polyphenols, may affect nutrient release.Given the apparent weakness in the two approaches discussed above, there exists a strong argument for the integration of socio-economic variables in natural resource management decision trees. In this case, the challenges are there but not insurmountable. The first challenge is that these decision trees should not be seen as recipes for action but as sets of options which farmers can validate and 'ground truth' to suit their own individual circumstances. This requires a joint learning process between researchers and farmers. The immediate challenge is to determine the most suitable ways in which this joint learning can take place in terms of what tools and platforms to use. There is an urgent need to test a range of practical tools to communicate scientific principles to farmers through use of 'farmer friendly decision trees'. This study is an attempt to derive decision guides for manure use. The objective of formulating the decision guides were two fold:1.to document how farmers use the available manure and, 2.to use the decision guide as a tool to identify opportunities in manure management and enhancement of soil fertility.The study was an attempt to come up with a framework for developing manure decision guides based on both research and farmer perceptions and understanding. The starting point for the study pivoted around the key question of what type of guide should be developed and the target clientele. Would there be separate guides for research and extension and one for farmers? For which nutrients and for what crops? An even more important question was how do we integrate what farmers are doing into research recommendations? Are research recommendations and farmer perception compatible? In this regard it was important to establish farmers' characterization of quality-especially the range of manure qualities found.A draft guide was synthesized from available research information by the authors to design a single framework representing the essential elements required in providing researchers and extension with a tool for manure management. The framework was further distilled through peer review after which it was field-tested with a group of 50 farmers in Mfiri village, Shurugwi District, Zimbabwe. Testing of the decision guide was carried out to identify gaps and weaknesses in its thought flow (presentation) and content. The decision guide was presented to farmers and the field testing of the guide took three forms: 1.eliciting information to improve the guide in group discussions, 2.eliciting information on decision criteria used by farmers when using manure (focus group discussions to develop a farmer guide), and, 3.Personal interviews to get information on specific household management practices. In order to incorporate farmer perceptions into the design of the guide, focus discussions to find out current manure use practices were held with discussions centered on manure quantities available, quality, curing methods, methods of application and supplementation. Household interviews were conducted with four farm families that were randomly picked from the large group of almost 50 farmers. The sample size was small, but nevertheless targeted at bringing out the diversity in individual practice and the elements that farmers consider being important for inclusion in a decision guide. These personal interviews provided an opportunity to explore further the issues that came up from the large group discussions.After the meetings with farmers, the results of the field testing of the guide were evaluated and changes incorporated into the research and extension guide. A second framework was developed based on the discussions held with farmers. A spidogram or web analysis was used as a tool for eliciting and summarizing information on the management framework used by farmers. The web analysis allowed participants to draw out the complex, inter-linked relationships among effects, causes or factors during decision making.The challenge in developing research and extension guides is making sure that they are technically precise. Scientific insights into mineral release and fertilizer equivalence require that for such a guide to be useful, it must differentiate the use of different quality manures and the conditions that lead to those differences in quality. It came out clearly in the study that other useful categorizations in the research guide could be based on socio-economic variables such as livestock ownership (numbers owned and access to manure) as well as biophysical factors like use and type of residues in kraals, type of feed, quantity, storage and chemical characteristics which influences manure quality. An interesting result of the survey was farmers have their own indicators of quality and these include presence of moulds, colour, compactness, lumpiness, and texture. These indicators are qualitative but are useful to incorporate in a technical guide.Rates of application of manure are very variable even for the same farmer. There are seasonal variations of rate based on previous crop performance, quantities of manure available and manure rotations/cycles within a field. The frequency of manure application ranged from two to four years depending on the number of livestock hence quantity of manure a farmer had, and the residual effects of previously applied manure (Table 1). An interesting point that came from the discussions with farmers was that all available manure was used irrespective of quality but that use practice could be dependent on quality. From the personal interviews it was clear from some farmers that poor quality manure was most often broadcast whereas high quality manure was banded. Fields considered as breadbaskets are targeted for manure application. Some farmers use high rates of application if quality was poor and where fertilizer is available they supplement with large rates of nitrogenous fertilizer. Rate of fertilizer supplementation depends on crop performance and available income to purchase inorganic fertilizer.Using the above information from farmers and from secondary data combined with scientific understanding of nutrient management, a research and extension guide was developed (Fig. 1). The guide has two major components; the first (in the upper box) focussing on manure production and storage and their impact on quality, and the second emphasizing management of different quality manure. The guide is not crop specific but there is implicit recognition that farmers in Zimbabwe where the guide is initially targeted give priority to maize and vegetables. The management systems that the guide attempts to address are complex and depend on individual decision making and the soil type at any particular location and other household labor limitations.The results presented in this section were from discussions with a focus group involved in analyzing decision criteria. Decision-making by the farmers was analyzed through a spidogram approach culminating in the development of a farmer derived decision framework (Fig. 2). One farmer presented the guide to the larger group of farmers after which it was further refined. The salient features of the farmer decision guide were that: 1.Farmers have a range of options that they use for soil fertility improvement as shown in the upper sections (A) of the diagram in Fig 2 . 2.Farmers value quantity as being more important than quality of manure. This could be an indication that farmers are primarily more interested in the amount of resources they have and the extent to which they can be spread around over the farm. This seems rational as the larger the quantity, albeit of poor quality manure, the more nutrients they will be adding to the soil. There are also other secondary benefits of organic matter addition such as increase in water holding capacity, increased nutrient use efficiencies etc. As a result, farmers have developed several strategies to improve the quantity of manure obtained from their kraals (Fig. 2, B). These include adding anthill soil, crop residues, leaf litter etc. From the discussions there appeared to be a strong realization by farmers that management affects quality. Farmers however do not deliberately manage or manipulate quality of manure to target it to a specific crop even though they might have preferences of which crop to apply higher quality manure (Fig 2,C and D). Residues are added primarily to increase quantity though they may have a secondary effect on manure quality. There is a limit to which residues can be added to the kraal, however. 3.Farmers are vague on rates, which maybe a reflection of a lack of consensus within a large group but also of wide differences between households. However they have guides on how to target manure application. 4. The quantity of manure that is pit stored is likely to remain small as some farmers feel that they have limited labor available.The farmer framework is much more comprehensive than the research and extension guide and probably fits more within their environment. Concerns were raised that the farmer guide is more likely to vary from area to area. A useful decision guide should be generic allowing for modifications to be made as circumstance change. The farmer guide was useful as a training tool to let farmers be more aware of the need to manage quality as well rather than quantity of manure alone. There was broad consensus that the research extension guide should be technically precise.There is a big gap that needs to be filled on farmer quality characterization. It will be necessary to more thoroughly ascertain the range of manure qualities that are identifiable by farmers, and how the identifiable indicators differ with region, and whether those indicators are socially differentiated. A pertinent question for research is whether there is a clear color pattern associated with stage of decomposition? If so then this opens up the potential to use color charts as indicators of quality and hence of how a particular manure could be managed. There is need to look at farmer indicators and how they relate to laboratory indices. Farmer quality indices might need to be considered in combination and not individually.The farmer-developed manure guide still looks complex despite the fact that it was presented by a farmer to a larger audience of farmers. Opportunities for the further simplifying it should be identified. There is no doubt that farmers were excited by the manure decision guide hence it would be worthwhile to expand the range of decision guides that could be developed for the many options that farmers have for soil fertility improvement (Fig. 2). Whilst a singular decision tree for a particular option forms the best way of exploring the way it is managed and how its management can be improved, farmers are most often managing combinations of different resources. The challenge therefore remains of how to integrate use of different decision trees for optimal management of multiple resources. For example, how do legumes fit within intervening years of the manure cycle etc. Similarly, a range of platforms and learning spaces suitable for different categories of farmers should also be identified and tested (these could range from nutrient test strips to village labs and pot experiments).A lot of issues have been raised above. Critical among those issues is to continue testing the decision guides and ensure that they are robust and applicable (but not necessarily) to a wide range of environments without losing the context of farmer circumstances. The question of validation should be left to farmers while the main role of the scientist should be that of facilitating this process of validation. Validating decision trees should not be viewed more in terms of going out in the field to prove a scientific point, but rather in terms of enabling farmers to test our scientific models.In order for farmers to validate decision trees, they must understand the basic scientific principles from which scientists derived them, and this can only be done through a process of researcher farmer dialogue and mutual learning. By the same token the joint learning process should also enable scientists to refine and adjust their scientific models by closely observing how and why different farmers are making their choices. Soil organic matter (SOM) plays an important role in determining the fertility and productivity of soils and hence the need to understand more clearly the factors that control SOM dynamics as affected by land use management practices. The association of organic matter with particular constituents of the mineral soil may be important in regulating the mineralization and storage of SOM. Pools of organic matter with different stabilities provide a spectrum of nutrient availability that have different rates of release and are susceptible to different kinds of disturbance (Woomer et al., 1994). The ability to quantitatively estimate SOM fractions is important for understanding SOM dynamics in agricultural systems.Several chemical and physical methods have been developed to separate SOM fractions. Chemical separation methods yield fractions that are not closely related to functions of SOM (Blair et al., 1997) to soil processes such as aggregation and organic matter mineralization (Stevenson and Elliot, 1989;Feller and Beare, 1997). These methods give information on the kind of organic matter present that may vary in age and N content (Duxbury et al., 1989). Chemical fractions (humic and fulvic acids) generally have a low turnover rate and are therefore not necessarily implicated in the short-term processes commonly studied in cultivated soils (Feller and Beare, 1997). Physical fractionation yields functional SOM pools which differ in composition and biological function as they give information on where the organic matter is located (Elliot and Cambardella, 1991).The effectiveness of soil dispersion with minimum alteration of associated organic matter, is crucial for physical fractionation. Limited dispersion of soil may result in the recovery of the most easily dispersed part of the fraction, or the recovered fraction may consist of an unknown mixture of primary particles and microaggregates of the same size but belonging to different size classes (Sanchez et al. 1989). Soil disruption is more rapid and complete with sandy soils than with heavy textured soils (Stevenson and Elliot, 1989). Soil dispersion can be achieved by sonication or shaking. Sonication can achieve high dispersion but it results in the breakdown of organic matter into finer particles (Feller and Beare, 1997) and produces heat, which might alter organic matter composition (Stevenson and Elliot, 1989). Shaking reduces organic matter redistribution. It can be done in water with or without glass beads, or after chemical pre-treatment of soil with sodium saturated chemicals.The work reported in this paper is part of a broader study to determine the effects of tillage on SOM dynamics in a long-term experiment. The experiment was carried out at two sites in Zimbabwe, on a sandy soil (Udic Kandiustalf-USDA) at Domboshawa and on a red clayey soil (Rhodic Paleustalf-USDA) at the Institute of Agricultural Engineering (IAE) in Hatcliffe Harare. Before following the dynamics of SOM in these soils initial studies were conducted to test and establish methods for the fractionation of SOM. The methods tested are based on similar principles (physical-particle size separations) but use different dispersing agents. The degree of dispersion of aggregates was used as the criterion for choosing one method.The objectives of this experiment were to assess the effectiveness of soil dispersion for two soil types (a sand and a red clay soil) under different tillage treatments by a) using sodium resin bags and sodium hexametaphosphate, b) two concentrations of sodium hexametaphosphate (HMP) 0.5 % and 2%, and c) pre-soaking the soil before shaking. It was hypothesized that a) higher dispersion would be achieved with the use of sodium resin bags than with sodium hexametaphosphate, b) increasing the concentration of sodium hexametaphoshate would increase the degree of dispersion, and c) pre-soaking the soil before shaking would increase soil dispersion.The tillage experiments that were established in 1988/89 season at Domboshawa and Institute of Agricultural Engineering (IAE), Harare were used for this study. There were five tillage treatments, mulch ripping, conventional tillage, tied ridging and clean ripping. Soil samples were collected in October 1998 and passed through a 2 mm sieve.Soil dispersion was done in sodium hexametaphosphate at two concentrations, 0.5% and 2%, and sodium resin bags, which were regenerated in 3M trisodium citrate. For soil dispersed in 2% sodium hexametaphosphate one set of samples was soaked in water overnight before shaking while for the other set there was no soaking. Fractionation was carried out by sieving the soil through two sieves to get the following size fractions, size fractions 212-2000 μm, 53-212 μm and 0-53 μm. The 53-212 μm size fraction of soil shaken in sodium resin bags was shaken for 1hr in resin beads to allow for further disruption of aggregates. The fractions were viewed under a binocular microscope to check for purity of the fractions and degree of dispersion of aggregates.The 212-2000 μm and 53-212 μm organic matter fractions were analysed for organic carbon using a Leco Carbon Analyser. Organic C in the 0-53 μm fraction was not analysed for organic C.T-tests for paired observations using Genstat 5 Release 4.1 were done to test for differences in using the different dispersing agents.For the red clayey soil none of the dispersing treatments achieved complete dispersion. This was observed under a binocular microscope, by the presence of micro-aggregates in the mineral fractions and mineral particles coating the organic matter fractions. For the sandy soil, however, complete dispersion of aggregates was achieved with all the dispersing agents used. The mineral fractions did not show the presence of micro-aggregates while the organic matter fractions were not coated with mineral fractions.Sodium resin bags and sodium hexametaphosphate were effective in achieving complete soil dispersion in the sandy soil. There were no significant differences in the amount of fractions separated using the different dispersing agents except for the coarse organic matter fractions separated using the resin bags. There was a reduction in the amount of coarse sand organic matter fraction separated using resin bags compared with sodium hexametaphosphate (Table 1).For the clayey soil none of the dispersing treatments used achieved complete dispersion. The use of resin bags increased dispersion of aggregates but reduced the amount of coarse organic matter recovered compared to sodium hexametaphosphate (Table 1). There was a slight but not significant decrease in the coarse mineral fraction and an increase in the intermediate mineral and mixed fractions obtained with the resin bags compared with sodium hexametaphosphate. There were significant differences in amounts of fractions dispersed in 2% hexametaphosphate and resin bags. Sodium hexametaphosphate gave higher amounts of the coarser fractions than resin bags (Table 1).Increasing the concentration of sodium hexametaphosphate from 0.5 to 2% did not result in an increase in soil dispersion for the red clayey soil. There were no significant differences in the mass of the organic matter and mineral fractions obtained using the two concentrations.The reduced amount of organic matter after using resin bags might have been a result of purer fractions being obtained. To see if the reduction in the amount of organic matter was a result of an increase in purity of the organic matter fractions, the fractions were analysed for organic C. The use of resin bags resulted in the reduced amount of organic C in the coarse organic matter fractions than sodium hexametaphospahte (Table 2).For the red clay soil pre-soaking gave lower amounts of the mineral fraction with fewer aggregates than without soaking for separations done in 2% sodium hexametaphosphate (Table 3). Soaking did not however result in differences in the coarse organic matter fractions. Soaking did not result in differences in the masses of fractions obtained for the sandy soil.The effectiveness of aggregate dispersion was not affected by the different tillage treatments. The response of the different tillage treatments was the same across all the dispersing agents used.For the red clay soil conventional tillage and clean ripping had similar amounts of coarse and medium sand organic matter fractions although clean ripping had higher amounts of total organic C than conventional tillage (Table 4). In the fine sand fraction clean ripping had higher amounts of organic matter than conventional tillage. Mulch ripping had higher total organic C, coarse and medium sand fraction organic matter contents than clean ripping (Table 4). Tied ridging had the highest total organic C (20.4 mg C g -1 soil) and sand fractions organic matter contents in the red clay soil (Table 4).Bare fallow showed the highest decline in soil organic matter content as indicated by the lowest total organic C content (2.2 mg C g -1 soil) and smaller amounts of organic matter in all the organic matter fractions separated (Table 5). Conventional tillage had low total organic C content and low amounts of organic matter sand size fractions. Mulch ripping had higher total organic C amounts of sand size fractions than clean ripping (Table 5). Tied ridging had similar total organic C and amounts of organic matter in the sand fractions with clean ripping (Table 5). Hand hoeing had lower total organic C content and organic matter in the sand fractions than mulch ripping except for the fine sand fraction where there were no treatment differences (Table 5).There was no complete dispersion for the red clayey soil probably due to the high clay content (>40%) which results in the formation of strong bonds between mineral and organic particles to form micro-and macro-aggregates. Clay particles have high surface area and tend to form strong mineral-mineral and ogano-mineral interactions that require high force to disrupt. In the sandy soil there was complete dispersion due to the presence of weak aggregates that require minimal force to disrupt. Elliot and Stevenson (1989) also found that disruption was more rapid and complete with sandy soils than with heavy textured ones. Sandy soils tend to have weak structure due to high sand content, which has low surface area, no charge and no hydrogen bonding for formation of aggregates (REF).Resin bags and sodium hexametaphosphate were effective in dispersing the sandy soil as complete dispersion was achieved. Both dispersing agents were however not effective in dispersing the red clayey soil. This was probably due to the strong interactions in the micro-aggregates such that there might be need for stronger forces to break down the aggregates.Although the use of resin bags led to an increase in the dispersion of aggregates, it also led to a significant decrease in the amount of organic matter recovered. The use of resin bags could have caused further breakdown of the coarse organic matter or solubilisation of the organic matter. The material used to make the resin bags has a mesh size of about 150 μm such that some material may have been entrapped inside the bags and was difficult to wash out resulting in erroneous results and reducing the effectiveness of the regeneration of the resin bags.There was no increase in dispersion caused by increasing the concentration of the hexametaphosphate from 0.5 to 2%. More effective dispersion might be achieved at higher concentrations of the salt, but hexametaphophate does not readily dissolve making it difficult to make higher concentrations. Sodium hexametaphosphate is likely to result in salt interacts with the fractions during mineralization making it unsuitable to use if incubation studies are to follow.The use of resin bags resulted in smaller amount of organic C in the coarse organic matter fractions and redistribution into finer fractions. The redistribution of organic matter among size fractions complicates efforts to selectively isolate organic matter fractions from specific sources in the soil confusing interpretation of results. The finer fractions were not analyzed in this preliminary test. Complete dispersion was not achieved for the clay soil such that the mineral fraction contained some micro-aggregates and a significant amount of organic C is found within micro-aggregates (Stevenson and Elliot, 1989).Soaking the soil before shaking led to an increase in the dispersion of aggregates (Table 2). Soaking did not affect the coarse organic matter fraction as it is not found in close association with the soil and hence is easy to disperse. There was a decrease in the amount of the coarse mineral fraction after soaking the soil as more aggregates were disrupted and the bonds in the aggregates weakened. The larger amounts of the mineral fractions in dispersion without soaking was due to the inclusion of aggregates, many aggregate classes have the same size range as sand (0.05-2mm). Increased dispersion after soaking led to an increase in the amount of the fraction less than 53um as the finer fractions that make up the aggregates were dispersed.The lack of differences in the sand size fractions organic matter contents between conventional tillage and clean ripping (Table 4) for the red clay soil could have been because no organic residues were added to the soil for both treatments. Clean ripping had higher organic matter contents in the finer fractions than conventional tillage probably due to aggregate disruption that occurs under conventional tillage causing higher organic matter decomposition. Mulch ripping had higher organic matter contents in the coarse and medium sand fractions than clean ripping because of organic residues that are added to the soil. Tied ridging had the highest amounts of organic matter probably because there was minimum tillage involved. The ridges were established at the onset of the experiment in 1988/89 season with opening of holes for planting such that there was minimum disruption of aggregates.For the sandy soil bare fallow had lowest amounts of organic matter most probably because there were no organic residues added to the soil. The treatment involved annual ploughing without planting anything to the soil causing soil organic matter loss through runoff and erosion. Mulch ripping had the highest amounts of organic matter unlike the red clay where tied ridging had the highest amounts of organic matter. This was probably because of additions of organic residues under mulch ripping. The clay content of the sand was very low (<6%) such that there was no organic matter stabilisation between and within soil aggregates and the finer soil particles.Of the methods tested using 0.5% sodium hexametaphosphate after soaking the soil would be the most suitable dispersing agent to use for getting high dispersion without altering organic matter distribution in the various size fractions for the red clayey soil and the sandy soil. This was so because increasing the concentration of sodium hexametaphosphate did not increase soil dispersion for the red clayey soil. Soaking the soil on the other hand resulted in an increase in disruption of aggregates by decreasing the mass of the mineral fraction by about 30%. Using resin bags resulted in low recoveries of the amount of organic C and mass of organic matter fractions compared with sodium hexametaphosphate and hence its selection. Different storage conditions influence both carbon and nitrogen turnover of the manures after application to soil. During aerobic composting organic materials of high stability are formed and the inorganic N can be low. Anaerobic decomposition can lead to the production of low-molecular compounds such as volatile fatty acids and high ammonium-N contents have been reported (Thomsen, 2000). The addition of anaerobically decomposed manure to soil has been found to immobilise N due to the presence of easily decomposable C sources as observed in the work of Bernal and Kirchmann, (1992), Flowers and Arnold (1983) and Sims, (1986), with pig slurry and Murwira, (1993) with cattle manure. The dynamics of C or N mineralisation of organic manures in soils have been described by first-order reaction kinetics (Chae and Tabatabai, 1986 or a set of first-order reactions (Gale and Gilmour, (1986).In the present paper, we report the results of a study on N release patterns from aerobic and anaerobic manures treated in April and July with or without straw after application to a sandy soil The aim of the investigation was to establish possible differences in short-term N turnover rates in aerobic and anaerobic manures applied to soil and implications to the cropping system..Manures used in the study were obtained from four storage treatments namely pit manure (anaerobically decomposed) with or without straw; heap manure (aerobically composted) with or without straw. The manures had been composted in April 1999 and replicated in July 1999. Samples of manure were collected in October1999 and air dried then ground to pass a 2-mm sieve, analysed for organic C (Nelson and Sommers, 1982) total N using the Kjeldahl procedure (Bremner and Mulvaney, 1982).The chemical composition of these manures is presented in Table 1.The experimental soil was collected from a site in Murewa. The soil was a deeply weathered and leached loamy sand with 4% clay, 4% silt, 92% sand and a pH of 4.5 (CaCl 2 ) classified as a Haplic Lixisol (FAO), Nyamapfene, (1991). The soil was air dried and allowed to pass through a 2mm sieve and analysed for organic C using the Walkely and Black Method (Nelson and Sommers, 1992) and total N using the Kjeldahl procedure (Bremner and Mulvaney, 1982).The aerobic leaching tube mineralisation method (modified from Stanford and Smith, 1972) was used in this study. This method was employed to reduce the number of tubes that would be required if the static method was to be used. An added advantage of the leaching tube method is that it mimics the field situation where N is constantly removed from the soil crop system through crop uptake and leaching. The tubes can also be used over a long period without experiencing problems of accumulation of N and other toxic products of decomposition (Bremner, 1965;Stanford and Smith, 1972). One disadvantage of the leaching tube is that, it removes the soluble carbon, which drives the mineralisation process.The leaching tubes consisted of a plastic transparent cylinder (200ml by volume) with a hole (0.5mm diameter) at the bottom. A narrow glass tubing (10cm long) was inserted at the base through the hole to allow drainage and secured with plasticine at the base. A glass wool 3cm thick was placed at the base of the tube to prevent movement of soil-manure particles from draining out of the tube.Ten grams of acid washed sand was added on top of the glass wool before adding soil-manure mixture to ensure even distribution of suction. A further 10g of acid washed sand was added at the top of the soil-manure mixture to avoid movement of particles upon pouring leaching solution.To reduce moisture loss, a pierced aluminum foil was placed on top of the tube covering it loosely. A mass of 100g of soil was placed in each glass jar. This was mixed homogeneously with 8 different manures applied at quantities equivalent to 60 kg N/ha and transferred into leaching tubes. A set of control tubes with 100g of soil only was added. All treatments were replicated 4 times. Moisture content was adjusted to 70% of water holding capacity (WHC) and the tubes were incubated in a constant temperature room at 25 o C.The tubes were leached at day 0 to remove background mineral N. They were then leached on day 3, 7 and every week thereafter for a period of 11 weeks. The tubes were leached with 100ml of a leaching solution in two 50ml aliquots and the leachates collected in conical flasks. The leaching solution contained 1mM CaCl 2 , 1mM MgSO 4 , 0.1 mM KH 2 PO 4 and 0.9 mM KCI. At each leaching date, moisture content was adjusted to 70% of WHC.Concentrations of mineral N (NH 4 -N plus NO 3 -N) in the leachates were measured immediately after sampling using micro Kjedhal N method. The mineralisation of manure nitrogen was determined by subtraction of the amount of inorganic nitrogen mineralized in the soil (control) from total inorganic N amounts mineralized in the soil-manure mixture.Cumulative data on N mineralisation were fitted to a first order kinetic function using Genstat 5 procedures. Analysis of variance procedure using Mstat 1988 was used to measure the significance of net mineralisation during the incubation period.The chemical composition of manures is presented in Tables 1. Anaerobic manures were always high in total N concentrations. A greater proportion of the total N in these manures was present as inorganic N in the form of NH 4 -N. Highest concentrations were observed for JP-manures. The C/N ratio for anaerobic manures was high, between 14 and 19 compared with 8 -13 for aerobic manures.Net N mineralisation from manures (without soil) Fig. The course of mineralisation/immobilisation significantly differed (P<0.05) according to the different storage treatments applied to the manure samples prior to incubation. In general, there was also a significant month * straw interaction and straw * storage method interaction (P<0.05). Straw effect was significant (P<0.05) and amplified in anaerobic manures in July.Anaerobic manures resulted in shorter periods of rapid initial immobilisation phase lasting between 4 and 5weeks Fig . 1. In general, the immobilisation period was increased in manures with straw. Aerobically treated manures resulted in immobilisation, which lasted 7 weeks after incubation for manures with and without straw (Fig 1).In terms of net N mineralisation, anaerobic manures had significantly higher net N mineralisation at 11 weeks after incubation than aerobic manures (P<0.05). July anaerobic manures were found to have significantly higher net N mineralisation than April manures. A release of 48 and 58 ugN -1 g soil of mineralisable N was observed from anaerobic manures with and without straw in July at 11 weeks. April anaerobic manures with and without straw released 25 and 30ug N g -1 soil of mineralisable N at 11 weeks. Anaerobic manures achieved a net N mineralisation of less than 20ug N g -1 soil for manures from this farm (Fig 1).The cumulative N curves of aerobic and anaerobic manures were fitted to a first-order kinetic function with the rate constants describing the release of mineral N (Fig 2). Mineralisation was expressed as a percentage of the amount of total manure-N added. After 77 days of incubation, N mineralised showed the following pattern: JP-(30.65%) > JP+ (25.59%) > AP-(22.75%) > AP+ (22. 60%) > JH-= JH+ =AH-= AH+ (0.00%). The order of percentages of total N that was mineralised over 77 days of incubation followed that of the rate constants for the slow re-mineralisation phase High coefficients of determination were found (R2 = 0.939 -0.990). (Table 2).In general, all aerobic manures followed a linear course for either nitrogen immobilisation or mineralisation which, lasted over the whole experimental period of 77 days. The rate constants were low for aerobic manures as shown in Table 2. The decomposition of anaerobic manures was characterised by two different phases, a rapid exponential initial immobilisation phase followed by a slow linear remineralisation phase.The rate constants for the slow re-mineralisation phase exhibited the following pattern: JP-(0.068 N day -1 ) > JP+ (0.058 N day -1 ) > AP-= AP+ (0.05 N day -1 ) > JH-(0.038 N day -1 ) > JH+ (0.028 N day -1 ) > AH-= AH+ >0.00 N day -1 ).Initial immobilisation effects by anaerobic pretreated manures were observed in this study. The reason appeared to be three-fold; the high C/N ratio greater than 15 found in anaerobic manures which was similar to that reported by Bernal and Kirchmann, (1992) and Castellanos and Pratt, (1981). Secondly, high microbial activity, which causes a shift in microbial population from predominantly anaerobic bacteria resulting in a flush of readily available carbon and consequently more C utilisation for microbial proliferation (Thomsen and OsIen, 2000). Thirdly, the presence of energy-rich easily degradable C compounds by microorganisms such as volatile fatty acids (Spoelstra, 1979) (though not measured in this study), when a shift into aerobic conditions occurred. The work of Paul and Beauchamp, (1989) showed that volatile fatty acids in slurry can be oxidized within 4 days after amending soil with anaerobic manure together with a parallel immobilisation of NH 4 -N. Earlier findings reported by Sims (1986) and Flowers and Arnold, (1983) found that up to 40% of NH 4 -N in anaerobic manures can be immobilised.The present data on prolonged periods of N immobilisation in soil with aerobic manures can be attributed to the stability of the organic materials in these manures. This is because easily decomposable organic compounds are respired during aerobic composting phase (Sana and Soliva, 1987). For example, water soluble and easily hydrolysable sugars are reduced during composting. Immobilisation of N was also possible with aerobic manures because a greater proportion of N was organically bound These results imply that the application of aerobic manures to soil could induce N deficiency during rapid crop growth leading to depressed yields (Murwira and Kirchmann, 1993;Nyamangara et al., 1999;Paul and Beauchamp, 1994). These workers found N release from aerobic manures to be asynchronous with maize crop N requirements. The results from this study have also been demonstrated in the work of Thomsen, (2000), Hadas and Portnoy, (1994) and Hadas et al., (1996). Their findings showed low N mineralisation rates for composted manures as reported in this study.In spite of the initial immobilistion, which occurred in soil with anaerobic manures, re-mineralisation of the inorganic N, occurred with these manures achieving highest rate constants with July stored manures and subsequently more inorganic N was released than similar manures stored in April. These differences can be attributed to a decrease in mineralisation rates with length of storage as shown by Bernal et al., (1998) and more readily decomposable organic forms were converted to stable forms with prolonged duration of storage (Castellanos and Pratt, 1981;Chaney, Drinkwater and Pettygrove, 1992;Kirchmann, 1985). The re-mineralisation of N that occurred with anaerobic manures during the fifth and sixth weeks after incubation is in agreement with the work reported by Murwira and Kirchmann, (1993).The results in this study contradict findings reported by Thomsen and Oslen, (2000) in which soils with anaerobic manures showed net immobilisation only after 266 days of incubation. Because of high microbial proliferation that occurs after application of anaerobic manures, these workers suggested that it might be more difficult to synchronise N release from anaerobic manures with crop N demand. However, in the present study, re-mineralisation of the inorganic N occurred close to the rapid crop growth stage between the fourth and sixth weeks after incubation. This implies that the release of N from these manures can be synchronised with crop N requirements.Because of slow mineralisation rates found in soil after application of aerobic manures, crop yield potentials in the short term can be adversely affected. This might imply that aerobic manures are only beneficial to the crop in the subsequent years after application. (Tanner and Mugwira, 1984;Paul and Beauchamp, 1994).Results showed significant variations in the decomposition of manures from different storage conditions. Differences in the rate constants between the manures reflected initial short term variations in the inorganic-N content of the readily decomposable fractions. Anaerobic manures with their high initial NH 4 -N contents were found to have highest rate constants than aerobic manures.The decomposition of anaerobic manures in soil almost always resulted in temporal initial immobilization. The immobilisation period was lengthened in manures with straw and by the age of manure owing to duration of storage. In spite of the initial immobilisation of the inorganic N that occurred in soil with these manures, re-mineralisation occurred close to the rapid crop growth stage reflecting that these manures may be synchronised with crop N requirements in the short term.Little or no N was mineralised from aerobic manures. The implications are that these manures could be an inefficient source of fertiliser N for the crop. Though N released by these manures may be asynchronous with maize crop N requirements in the short term, the proportion of the N that still remains in organic bound form could be available for transformation in the residual years. T im e (D a y s )Cumulative N (ugN g -1 soil)J H -J H + J P -The objective of this study was to assess tillage effects on SOM content and SOM fractions on two different soil textures. It was hypothesised that minimum tillage practices would promote SOM buildup, especially the physically protected organic matter, compared with conventional tillage systems.This experiment was done on the tillage experiments that were established in the 1988/89 season at the Institute of Agricultural Engineering (IAE) in Harare (17 o 45' S; 31 o 10'E) and Domboshawa Training Centre (DTC) (17 o 35' S; 31 o 10'E) approximately 40 km NE of Harare. The IAE site is on red clay soil derived from gabbro parent material and is classified as Rhodic Paleustalf (USDA), Chromic Luvisol (FAO) and Harare 5E.2 (Zimbabwe). The DTC site is on a sandy soil derived from granitic parent material classified as Udic Kandiustalf (USDA), Areni-Gleyic Luvisol (FAO) and Harare 6G.3 (Zimbabwe). The clay mineralogy for both locations is predominantly kaolinite. Both sites are found in Natural Region II (annual rainfall 800-1000 mm) with most of the rain falling between November and March.The tillage treatments at the sites were as follows: 1. Mulch Rippingrip-between-row into residues (tine into residues) 2. Clean Rippingrip-between-row without residues (tine into bare soil) 3. Conventional tillageannual ox ploughing (single furrow mouldboard plough and spike harrow) 4. No-till tied ridgingpermanent crop ridges at 1 in 100 grades 5. Hand hoeingdigging out plant holes with a hand hoe without residues 6. Bare fallowannual tractor disc plough and disc harrow, no crops are grown At these sites there were annual fertiliser additions of 350 kg compound D (8% N, 14% P 2 O 5 , 7% K) and 250 kg ammonium nitrate (34.5% N) per hectare. The total amounts of nutrients added were 114 kg N, 50 kg P 2 O 5 (22 kg P) and 25 kg K per hectare. Maize was planted as the test crop.Soil samples were collected in October 1998 and passed through a 2 mm sieve. Fifty grams soil was shaken overnight in 200 ml of 2% sodium hexametaphosphate after soaking the soil overnight for 16 hours. Soil was wet sieved through a series of sieves to separate 212-2000 μm, 53-212 μm, 20-53 μm fractions followed by separation of organic and mineral fractions in each size fraction by swirling and floating of the organic matter in water. The 0-5 and 5-20 μm fractions were separated by the sedimentation method but were not separated for organic and mineral fractions. Carbon in the organic matter fractions and the mixed fractions was analysed using a Leco Carbon Analyser.Statistical analysis was done using GENSTAT 5 for analysis of variance (ANOVA).When comparing within tillage treatments, total organic C contents were higher in the red clay than the sandy soil (Tables 1 and 2). Total organic C was almost three times higher for the red clayey soil than the sandy soil for all the treatments. The weedy fallow had the highest total organic C contents for both the red clay and the sandy soil with the red clay soil having higher C contents (27.9 mg C g -1 soil) than the sandy soil (11.3 mg C g -1 soil) (Tables 1 and 2). Of the tillage treatments, tied ridging had the highest total organic C content with 20.4 mg C g -1 soil for the red clayey soil while for the sandy soil mulch ripping had the highest organic C content of 6.8 mg C g -1 soil. For both soils organic C content was lowest in the conventional tillage treatment with the red clay having 14.9 mg C g -1 soil and 4.2 mg C g -1 soil for the sandy soil.Coarse sand organic matter content (212-2000 μm) was higher in the red clayey soil than the sandy soil for the conventional and tied ridging treatments where organic matter content was almost twice as high in the former than in the latter (Tables 1 and 2). Coarse organic matter content was similar in the red clay and the sandy soil for the other treatments. Organic matter content for the medium sand (53-212 μm) fraction was almost twice as high in the red clay soil than in the sandy soil except in the tied ridging treatment where organic matter was more than seven times as high in the clay soil than in the sandy soil. For the fine sand (20-53 μm) fraction, organic matter content was almost three times as high for the red clay soil than for the sandy soil. As the fraction size decreased the difference between the amount of organic matter in the sand and the clay soil increased with more organic matter being found in the clay soil.Most of the organic matter was associated with the finer fractions for the red clay whilst for the sandy soil the greater proportion of the organic matter was associated with the coarse mineral (sand) particles. As the particle size decreased there was an increase in the amount of organic matter in the red clay soil whereas there was no difference in the amount of organic matter in the particle size fractions for the sandy soil (Tables 1 and 2).For the red clayey soil clean ripping and conventional tillage had similar amounts of coarse and medium sand organic matter fractions although clean ripping had higher organic C content than conventional tillage (Table 1). In the fine sand fraction clean ripping had higher amounts of organic matter (10.1 mg g -1 soil) than conventional tillage (8.0 mg g -1 soil). Mulch ripping had higher total C, coarse and medium organic matter contents than clean ripping (Table 1). The fine sand associated organic matter content of conventional tillage was not significantly less than that of clean ripping. Of the tillage treatments, tied ridging had the highest organic C content and amount of sand organic matter fractions in the red clayey soil (Table 1).Bare fallow resulted in the highest decline in soil organic matter content as indicated by the lowest total organic C content (2.2 mg C g -1 soil) and smaller amounts of organic matter in each of the size fractions (Table 2). Conventional tillage had low total organic C contents and low amounts of organic matter in the size fractions in the sandy soil. Mulch ripping had higher total organic C content and amounts of organic matter in the coarse-and medium-sand fractions than clean ripping (Table 2). However the amount of organic matter in the fine sand fraction was not significantly larger for the mulch ripping treatment compared with the clean ripping treatment. Total organic C and amounts of organic matter in the sand fractions for tied ridging were not significantly different from the clean ripping treatment except for the medium sand fraction where clean ripping (2.2 mg g -1 soil) had higher amounts of organic matter than tied ridging (1.5 mg g -1 soil) (Table 2). Hand hoeing had lower total organic C content and organic matter in the sand fractions than mulch ripping except for the fine sand fraction where there were no significant differences (Table 2).For both soils the highest decline in organic matter under the different tillage treatments was in the coarse sand organic matter fraction when compared with the weedy fallow (Tables 1 and 2). With decrease in the organic matter size fraction there was a decrease in the magnitude of the difference in the amounts of the organic matter fractions under different tillage treatments when compared with the weedy fallow.Cultivation of soil at the IAE site led to a decrease in total organic C and organic C in the organic matter size fractions. All tillage treatments led to a decrease in organic C distributed in the size fractions when compared with the reference point, the weedy fallow. The coarse sand organic matter fraction (212-2000 μm) showed the highest decline in organic C after cultivation from 4.47 mg g -1 soil for the weedy fallow to as low as 0.97 mg C g -1 soil for conventional tillage (Table 3). The 0-5 μm fraction showed the lowest decline in organic C under all the tillage treatments. This was shown by the small margin of difference of organic C in the 0-5 μm size fractions under the tillage treatments compared with the weedy fallow. The smallest decline in organic C was under tied ridging in the 0-5 μm organic matter size fraction where there was no significant difference between organic C in the weedy fallow (23.5 mg C g -1 soil) and tied ridging (18.8 mg C g -1 soil).Conventional tillage showed the highest decline in organic C in all the size fractions compared with the other tillage treatments and the weedy fallow. Tied ridging showed the least decline in organic C in all the organic matter size fractions (Table 3) as indicated by the high organic C in the organic matter size fractions compared with the other tillage treatments. Clean ripping had higher organic C contents in the organic matter size fractions except for the coarse sand organic matter fraction compared with conventional tillage but less than mulch ripping (Table 3).At the Domboshawa site the bare fallow showed the highest decline in organic C in all the organic matter size fractions. This was more pronounced in the sand organic matter fractions where bare fallow had 0.05 mg C g -1 soil compared with 2.85 mg C g -1 soil for the weedy fallow (Table 4). In the clay size fraction bare fallow had 1.64 mg C g -1 soil while the weedy fallow had 4.37 mg C g -1 soil. Mulch ripping treatment had higher organic C contents in the organic matter size fractions than tied ridging. Mulch ripping had higher organic C (0.92 mg C g -1 soil) than hand hoeing (0.63 mg C g -1 soil) in the sand fractions (20-2000 μm) but had lower organic C contents in the finer fractions.There was a differential treatment effect on total soil organic C and organic C in the organic matter size fractions caused by tillage for the two soils. Tied ridging had the highest organic C and C in the size fractions for the red clay soil (Table 3) and mulch ripping had the highest total organic C and C in the size fractions for the sandy soil (Table 4).When organic C in the organic matter size fractions for both soils was totalled, recoveries of total organic C were not 100%. Higher organic C recoveries were obtained for the sandy soil than for the red clay soil. Organic C recoveries averaged 85% for the red clay soil (Table 3) and 95% for the sandy soil (Table 4).Effects of texture on organic matter content and distribution in size fractions Total organic C was higher in the clay soil than in the sandy soil most likely due to lack of physical protection of organic matter from microbial attack in the sandy than in the clay soil, as well as larger residue inputs from roots due to greater productivity (Hassink, 1995;Hassink, 1996). The high clay content (~60% in the plough layer) in the clayey soil promotes formation of micro-and macro-aggregates which might physically protect soil organic matter from microbial decomposition and hence promote organic matter accumulation. This is unlike the sandy soil which has a low clay content (~5% in the plough layer) such that there is minimum aggregation and hence little organic matter accumulation. Hassink et al. (1997) observed a close relationship between silt and clay content, and organic C content of soil, with sandy soils having lower organic C in whole soil and fractions than clay soils.The difference of organic matter contents of the coarse sand fractions for the red clay and the sandy soil was small but the differences increased as the fraction size decreased. Organic matter in the finer fractions is protected from microbial decomposition and hence the increase in the margin of the difference in organic matter content as the fraction size decreases for the red clay soil compared with the sandy soil, mainly due to the higher proportion of finer fractions in the clay soil. As a result of this much of the organic matter in the clayey soil tends to be associated with the finer particles.Bare fallow involves ploughing of plots every year without planting anything and this enhances soil erosion resulting in soil organic matter loss. Ploughing the soil enhances organic matter decomposition by disrupting aggregate protected organic matter. This could have resulted in lower soil organic C and N when compared with the other tillage treatments. This supports the findings of Cambardella and Elliot (1994) who found that bare fallow soil had significantly less total organic C and N than mulch tillage and no-till soils. Cambardella and Elliot (1992) demonstrated that loss of coarse organic matter under a bare fallow treatment amounted to 70% of that of native grass over a period of twenty years.For the red clayey soil clean ripping and conventional tillage had similar organic matter contents in the coarse and medium sand fractions probably because no residues were added to the soil in both treatments and hence they received similar and small amounts of organic inputs. In the fine sand, however, clean ripping had higher organic matter contents than conventional tillage probably due to reduced tillage for the clean ripping treatment such that there was reduced disruption of the soil resulting in reduced organic matter decline compared with conventional tillage. The higher coarse sand organic matter content in the mulch ripping treatment (34.8 mg g -1 soil) compared with 26.8 mg g -1 soil in the clean ripping treatment was probably because of organic residues that are added on the surface for the mulch treatment (Table 1). Total soil organic C and organic matter in the finer fractions was not significantly different for the two treatments probably because both treatments involve minimum tillage such that organic matter in the finer fractions is not affected by tillage. The two treatments are similar in terms of tillage intensity hence organic matter in the finer fractions was similar. Since much of the organic matter for the clay soil is associated with the finer fractions the difference in total organic C content of the two treatments was not significantly different. Tied ridging involves planting of maize on permanent ridges where tillage is reduced to opening of planting holes such that there is minimum soil disruption and hence greater organic matter accumulation.Conventional tillage had low total organic C contents and organic C in the SOM fractions than conservation tillage practices mainly because of the high tillage intensity which enhance organic matter loss from the soil (Hassink, 1995) and small organic matter additions to the soil (Yang and Wander, 1999). Dalal et al. (1991) observed that in the top soil layers the interactive effects of zero tillage and returning of residues resulted in high organic C contents when compared with conventional tillage and zero tillage with residue burning. Work done by Arshad et al. (1990) showed that organic C and total N were 26% greater following 10 years of zero tillage compared with conventional tillage in the upper 7.5 cm of a silt loamy soil.Lower organic matter contents for conventional tillage when compared with the conservation tillage treatments were most likely a result of aggregate formation and turnover processes. Conservation tillage practices allow for slow macroaggregate turnover resulting in the formation of fine particulate organic matter and the subsequent encapsulation of the fine particulate organic matter by mineral particle and microbial by-products to form stable microaggregates (Six et al., 2000). In contrast the turnover of macroaggregates in conventional tillage is fast, providing less opportunity for the formation of crop derived fine particulate organic matter and stable microaggregates (Six et al., 2000).Mulch ripping had higher total organic C content (6.8 mg C g -1 soil) and organic matter in the coarse-(46.8 mg g -1 soil) and medium (42.6 mg g -1 soil) sand fractions than clean ripping for the sandy soil (Table 2). This was possibly due to the addition of organic residues under the mulch ripping treatment, which resulted in higher organic matter in the coarse-and medium sand fractions than clean ripping. This could have resulted in higher total organic matter under mulch ripping than clean ripping because much of the organic matter in the sandy soil is associated with sand particles and hence the larger sand size fraction organic matter with mulch ripping resulted in higher total organic matter content. Hand hoeing involves reduced tillage and hence had high organic matter contents although it was lower than mulch ripping.There was a decrease in organic C in the organic matter fractions and total soil organic C for all the tillage treatments on the two soils compared with the weedy fallow (Tables 3 and 4). This was maybe because cultivation disrupts aggregate protected organic matter and enhances its decomposition. The largest decline of organic C in the organic matter fractions was in the sand fractions under conventional tillage, 4.47 mg C g -1 soil for the weedy fallow compared with 0.97 mg C g -1 soil for conventional tillage on the red clay soil (Table 3) and 2.85 mg C g -1 soil for the weedy fallow compared with 0.05 mg C g -1 soil for conventional tillage on the sandy soil (Table 4). This was probably because of the absence of the annual litter additions under conventional tillage compared with the weedy fallow. Conventional tillage also involves intensive cultivation of the soil promoting soil organic matter decomposition and subsequent loss from the system. For all the tillage treatments, however, the largest organic C decline occurred in the coarse organic matter fractions probably as a result of physical disintegration of soil aggregates associated with SOM decomposition and mineralization (Tiessen and Stewart, 1983). Tied ridging showed the least decline in organic C in the 0-5 μm fraction probably due to minimum tillage practised under tied ridging resulting in minimal disruption of aggregates.These results confirm the findings of Cadisch et al. (1996) and Barrios, Buresh and Sprent (1996b), that coarse organic matter (light fraction) is an early indicator of changes in soil fertility and is the fraction most affected by cropping systems. Chan (1997); Hassink et al. (1997) also observed that the light fraction is lost more rapidly than other fractions. Under forest conditions, the light fraction was shown to be a strong short-term sink of N incorporating more than 50% of added N while the heavy (fine) fraction incorporated less than 5% after an 18 hour incubation (Compton and Boone, 2002). Work done by Lehmann et al. (1998), however, showed that short-term addition of Senna and Gliricidia leaves resulted in an increase in C and N in the silt and clay fractions.Bare fallow involves annual ploughing without planting anything without any input additions. This induces soil erosion which is associated with soil organic matter loss showing a large decline in organic C in all the organic matter fractions in the bare fallow treatment compared with the weedy fallow and other treatments (Table 4). Unlike for the red clayey soil, mulch ripping had higher organic C in all the organic matter fractions than tied ridging probably due to low clay content such that there is little physical protection of organic matter for the sandy soil.Results from this study indicate that there were higher soil organic matter losses under conventional tillage when compared with the other tillage treatments for both soils. This was maybe caused by the differing degrees of disruption of soil aggregates under the different management practices. Beare et al. (1994a) showed that after 13 years of conventional and no-tillage management resulted in 18% greater standing stock of soil organic C in the plough layer of no-tillage soil than conventional tillage soil. Beare et al. (1994b) also showed that the largest water stable aggregates were more abundant in the surface samples of no-till soil and that these aggregates were more stable and contained higher concentrations of C and N than did water stable aggregates under conventional tillage.Soil organic matter results were supported by maize yields and runoff loss results that were observed in the same experiment. Conservation tillage practices had higher maize yields and lower runoff losses than conventional tillage although there were seasonal variations. The variations were mainly a result of differences in the rainy seasons with some seasons being wet while some seasons were dry (Figs 1 and 2).In most seasons tied ridging gave higher grain yields for both soils because of the moisture benefits associated with tied ridging (Nehanda, 2000). The improvement in water use efficiency caused by tied ridging, which also had high organic matter contents could have possibly resulted in an increase in nutrient use efficiency resulting in higher yields. The higher maize yields under tied ridging were most likely associated with high root biomass additions to the soil and hence the high soil organic matter contents. In drier years conventional tillage gave higher yields because of less vigorous growth associated with crops under conventional tillage such that their moisture demands were lower. When however averaged across season, conventional tillage had the lowest maize yields. These results imply that conventional tillage causes faster soil degradation with increased soil organic matter decline, nutrient loss and susceptibility of soil to erosion, faster soil fertility decline and lower crop yields in the long run (Doran et al., 1987;Franzlubbers and Arshad, 1996;Feller and Beare, 1997). Higher grain yields were obtained for the red clay soil than the sandy soil due to the high water and nutrient holding capacity of the clay soil.Conventional tillage resulted in higher surface runoff for both soils because tillage loosens soil making it prone to rain drop impact and detachment (Figs 3 and 4).Conventional tillage had double surface runoff compared with the other tillage treatments while the other treatments had minimum surface runoff. The bare fallow treatment for sandy soil had the highest surface runoff losses because of the lack of a crop to cover the soil and protect the soil from being washed away from the soil surface. Surface runoff for the two soils was not different perhaps because the two sites receive similar rainfall. These results are similar to total C and organic C in the SOM fractions, where low organic C contents were observed under the bare fallow treatment and with high runoff losses, some of the organic could have been lost through erosion.At the IAE site conventional tillage led to a high decline in total organic C content (14.9 mg C g -1 soil) and organic C in the organic matter fractions compared with tied ridging (27.9 mg C g -1 soil) that promoted organic matter accumulation. For the sandy soil there was higher total organic C and organic C in the organic matter fractions under the mulch ripping treatment while conventional tillage led to higher organic C degradation. This means that tied ridging conserves organic C for the red clayey soil while mulch ripping conserves organic C in the sandy soil compared with the other tillage treatments tested. Higher organic matter loss was observed for the sandy soil with up to 61% organic C decline following conversion from the weedy fallow to conventional tillage. This was much higher compared with 47% organic C decline under the same conversion for the clayey soil. This means that sandy soils degrade faster than clayey soils under the same management practices because they have lower capacity to protect soil organic matter. Higher crop yields on the red clay soil also resulted in higher organic matter additions to the soil through root biomass compared with the sandy soil.Cultivation when compared to the weedy fallow results in the faster turnover and decline of organic matter especially in the coarse fractions. Conventional tillage resulted in faster losses of coarse organic matter than conservation tillage practices as there are no organic inputs and in the finer fractions because it does not allow for the formation of stable microaggregates. Land resource degradation is one of the major threats to food security and natural resource base in Ethiopia. Hundreds of years of exploitve traditional land use, aggravated by high human and livestock population density have led to the extraction of the natural capital, which caused the farming of uncultivable sloppy lands and overexploitation of slowly renewable resources. The outcome is that half of the highlands are eroded, of which 15% are so seriously degraded that it will be difficult to reverse them to be agriculturally productive in the near future. In the mountainous highlands, there is a direct link between land-based resources and rural livelihoods. Decline in soil fertility as a result of land degradation decreases crop/livestock productivity and hence household income. Depleted soils commonly reduce payoffs to agricultural investments, as they rarely respond to external inputs, such as mineral fertilizers, and hence reduce the efficiency and return of fertilizer use. Degraded soils have also very poor water holding capacity partly because of low soil organic matter content that in turn reduce the fertilizer use efficiency. There have been various attempts to reduce land degradation in Ethiopia since the 1970s, through national campaigns on construction of terraces, project afforstation programmes and policy interventions. The objective of this paper is to review the various research/development experiences on integrated soil fertility management and synthesize the positive experiences augumented by the experiences of the African highlands initiative on integrated land management in Ethiopian Highlands. The paper will also suggest an outline that could be used by farmers, researchers and policy makers to reverse the alarming trend of land degradation in the mountainous highlands. This work has consulted the available literature on land degradation and soil fertility management in Ethiopian highlands. While TSBF-CIAT/AHI has been working closely with the Ethiopian Agricultural Research Organisation (EARO) and the Buro of Agriculture, and conducting participatory research in two benchmark sites of the Ethiopian highlands on INRM issues, it became apparent that land degradation is the most fundamental threat for the Ethiopian Agriculture. Based on the systems intensification work that we have been conducting in the two benchmark sites of African highlands initiative, Areka and Ginchi, augmented by secondary data on relevant themes, the following approach was suggested to address land degradation in the country.There are multiple factors that cause land degradation at short and long terms in the region. In Sub Saharan Africa, the major bio-physical agents of land degradation are water erosion, wind erosion and chemical degradation that affected soil loss by 47, 36 and 12%, respectively. Given the mountainous and sloppy landscapes, the major environmental factor that causes considerable soil and nutrient loss within a short period of time is water erosion followed by wind erosion. Most of the Wollo and Shewa highlands became erosion-prone due to high rainfall intensity accompanied by very steeply farmlands. Recent surveys showed that erosion effect is severe in high rainfall areas predominantly covered by nitisols and vertisols. In about 40% of the highlands, the erosion effect was so severe that active erosion was transformed to passive erosion, and hence there are rarely visible signs of sheet or rill erosion, but gullies and land slides. The hazards of erosion in the region was accelerated by socio-economic factors, namely absence of land ownership rights that discourage long term investments, population pressure, lack of alternative income generating options, and weak social capital that failed to protect communal grazing lands, up-slope forest covers and water resources.Although the degree of soil erosion is highly related to the interaction of Wischmeier factors, the type of land use and management may have played an important role in the Ethiopian highlands. The contribution of different management factors towards land degradation in Africa is estimated to be 49%, 24%, 14%, 13% and 2% for overgrazing, agricultural activities, deforestation, overexploitation and industrial activities (Vanlauwe et al, 2002). The livestock sector is a very important component of the system both as an economic buffer in times of crop failure and economic crisis and as a supportive enterprise for crop production. There is a considerable concern, however, that the number of animals per household in Ethiopian highlands is much higher than the carrying capacity of land resources.Overgrazing due to very high livestock population density in the Amhara region is expected to contribute most to land degradation. For instance, the total annual feed available in the highlands is estimated to be about 9.1 million tones of biomass while the demand is about 21 million tones, double that of the carrying capacity of the land (Betru, 2002). Another very important factor that aggravated land degradation in the Ethiopian highlands is deforestation. The forest cover went down from 40% at the beginning of this century to less than 3% at present, due to ever-growing demand for wood products and very low commitment in planting trees mainly because of the prevailing nationalization of private woodlots in the 1970s and 1980s. Besides, a very high consumption of wood for fuel and housing, wood products, mainly charcoal, became a major cash generating activities in the country in recent years. Deforestation and overgrazing accelerated land degradation in many ways. Firstly a land without vegetative cover is easily susceptible to erosion, both wind and water, and hence causes a considerable nutrient movement. Secondly, a large amount of litter that could have contributed for maintaining soil organic matter and nutrient status is considerably reduced. Thirdly deforestation in the highlands caused lack of fuel wood, and hence farmers use manure and crop residue as cooking fuel, which otherwise could have been used for soil fertility replenishment. Over-mining of land resources with out returning the basic nutrients to the soil is also an important factor that contributed most for soil fertility decline in the region. For instance, barley is the single dominant crop in the upper highlands of Wollo. The system has very low crop diversity with legume component of less than 3%. The system receives external inputs very rarely with a fertilizer rate of less than 5 kg/ha (Quinones et al., 1997), and the practice of applying this limited amount of mineral fertilizer is a recent practice. Data from the region on the amount of nutrients returned to the soil in comparison to the nutrients lost through removal of crop harvest showed that only 18, 60 and 7 % of nitrogen, phosphorus and potassium is returned to the soil, respectively (Sanchez et al., 1997). Hence there is an over mining of nutrients from the same rhizosphere for years and years.Another cause of land degradation is lack of early awareness about land degradation by farmers, which is partly associated with the rural poverty. McDonagh, et al., (2001) reported that when farmers were asked to describe their indicators of soil erosion they stated gully/rill formation, exposed underground rocks, land slides, wash away of crops, shallowing of soils and siltation of the soil. Similarly farmers indicators of soil fertility decline include stunted crops, yellowing of crops, weed infestation, and change of soil color to red or grey. These are soil traits that appear in a much later stage of soil degradation, after the soil organic matter and nutrients of the soil are removed. If farmers respond to soil erosion at this stage, the probability of reversing the fertility status to its earlier value would be difficult.Application of small amounts of mineral fertilizer alone, as it has been practiced on the 0.5 ha demonstration plots by FAO and the ministry of Agriculture for years, did not improve crop productivity much. The failure of this mono-technology approach calls for an integrated nutrient management that suits local biophysical, social and economic realities. Integrated nutrient management technologies can be nutrient saving, such as in controlling erosion and recycling of crop residues, manure and other biomass, or nutrient adding, such as in applying mineral fertilizers and importing feed stuffs for livestock (Smaling and Braun, 1996).The traditional field operation in the Ethiopian highlands, which could be characterized by multiple tillage, cereal-dominated cropping and very few perennial components in the system, is very erosive for soils and nutrients. Continual farming in the high lands with out considering conservation measures caused severe land degradation. FAO study in Zimbabwe showed that each hectare of wellmanaged maize growing land lost 10 tones of soil. Depleted soils commonly reduce payoffs to agricultural investments for various reasons. Degraded soils rarely respond to external inputs, such as mineral fertilizers, and hence reduce the efficiency and return of fertilizer use. Degraded soils have also very poor water holding capacity partly because of low soil organic matter content that in turn reduce the fertilizer use efficiency. Results from the dry regions of Niger, Sadore, showed that application of fertilizer increased the millet yield by 71% and also improved the water use efficiency by 70% (Bationo et al., 1993). Hence improved soil fertility enhances the water use efficiency of crops in drought prone areas. Low soil organic matter accompanied by low soil water content may also reduce the bio-chemical activity of the soil that may affect the above and below ground biodiversity of the system. Degraded soils have also low vegetative cover that may accelerate further soil loss and runoff.The effect of soil fertility decline goes beyond nutrient and water losses. There are conviencing results showing that the incidence of some pests and disease is strongly associated with decline in soil fertility. Results from the Amhara and Tigrai region showed that the effect of the notorious parasitic weed, striga, on maize and sorghum was severe in nutrient depleted soil (Esilaba, et al, 2001). It was possible to decrease the population & the incidence of striga significantly by improving the fertility status of the soil through application of organic fertilizers. Similarly the incidence of root rots in beans, stem maggots in beans, take all in barely and wheat is associated with decline in soil fertility (Marschner, 1995). The positive effect of application of organic and inorganic fertilizer on the resistance of the host crop is mainly through improving the vigorosity of the plant at the early phonological stages. Amede et al., (2001) outlined the need for a combination of measures to reverse the trend of soil fertility decline in the African highlands as presented in the following section.There are about 40 different types of indigenous soil and water conservation practices in different parts of the Ethiopian highlands, ranging from narrow ditches on slopping fields in Wollo highlands to the most advanced & integrated conservation measures in Konso, Southern Ethiopia. However, those indigenous practices are location specific and variable in their effectiveness, and call for closer understanding before any attempt is done for scaling-up. However, there is a consensus among actors that any attempt to protect land resources and improve productivity in the sloppy highlands should integrate systemcompatible soil conservation measures. Research conducted in Andit tid and Gununo showed that increasing the vegetation cover of the soil could decreases soil loss and runoff significantly (SCRP, 1996). In Andit tid, the amount of soil loss due to water erosion was 230 t/ha/year under hacked plots. However, it was possible to reduce the soil loss to 30 t/ha or less under crop covers or fallow grasslands (SCRP, 1996). When a cropland covered by crops or grasslands is compared to a frequently hacked farmland, run-off was reduced by about 90 and 100 % and soil loss by 68%, respectively. Hence soil nutrient loss and runoff could be minimized through increasing the frequency of crop cover, especially by those crops with mulching habits and higher leaf area indexs. Moreover, results from SCRP showed that perennial crops like enset and fruit trees or annuals with mulching and runner habits could reduce erosion effects significantly. Recent simulation modules in Northern Ethiopia showed that crop lands allocated for cereal crops like teff were very prone to erosion (Woldu, 2002), and the authors proposed that growing small seeded cereals, like teff, in sloppy farmlands should be discouraged.There has been an attempt to control soil erosion and rehabilitate degraded lands through construction of farmland terraces in the Ethiopian Highlands starting from the early 1970s. The program was facilitated through the food-for-work scheme of the World Food Program, as a response to the frequent droughts of the 70s and 80s in Ethiopia. The program attempted to construct terraces on about 4 millions of hectares of farm land. In early 1990s, the annual physical construction of farmland terraces reached over 220,000 ha (Lakew, et al, 2000). However, as the campaign was trying to address the problem with out the full participation of the rural community, except selling labor, the farmers considered the activity as an external imposition and hence failed to develop sense of ownership. The consequence being that farmers failed to maintain the terraces and, in some case, farmers have destroyed the terraces for getting another round of payment. When farmers were asked to list the reasons for rejecting soil and water conservation technologies they listed five major driving forces (Amede, 2002, unpublished) namely high labor cost, decreased farm size due to terraces, its inconvenience during farm operations especially for U-turn of oxen plough, and inefficiency of the terraces to stop erosion as they were only physical structures without any biological component and technical follow-ups. By considering those farmers criteria and by adopting participatory planning and implementation approaches farmers have adopted and disseminated soil conservation technologies in one the African Highlands Initiative benchmark sites, Areka (Amede et al, 2001). The major driving force for the adoption of the technology was its integration with high value crops (e.g. bananas, hops) and fast growing drought resistant feeds (e.g. Elephant grass, pigeon pea) grown on the soil bunds. The sustainable integration soil & water conservation technologies also depend heavily on the effectiveness of by-laws that limit free grazing and free movement of animals especially during the dry spells. This requires the empowerment of the local and regional policies so as to facilitate the integration of natural resource management technologies to practices of local communities. Moreover, effective landscape management, in terms of controlling soil erosion, is possible only when there is a community collective action. Unless the landscape is treated as a single unit and involves all potential stakeholders, any individual intervention could provoke social conflicts. For instance, construction of soil conservation bunds and deforestation of forests at the upper slope of the Lushoto highlands, Tanzania, decreased the amount of water flew to the valley bottoms, and affected the vegetable production and income of other farmers.Building the organic matter of the soil and the nutrient stock in short period of time requires a systems approach. These include the combination of judicious use of mineral fertilizers, improved integration of crops and livestock, improved organic residue management through composting and application of farmyard manure, deliberate crop rotations, short term fallowing, cereal-legume intercropping and integration of green manures. Because of the inconsistent use of mineral fertilizers and the very limited returns of crop residues to the soil, most of the internal N cycling in small holder systems results from mineralization of soil organic N. Such process may contribute most of the N for the annual crops until the labile soil organic fraction (N-capital) are depleted (Sanchez et al., 1997).Apart from the occasional application of small amounts of mineral fertilisers, all other organic resources form the principal means of increasing soil nutrient stocks and hence soil fertility restorers in small-scale farms. If these approaches are used in combination and appropriately, they could reverse the trend and consequently increase crop yields and, thereby alleviate food insecurity. However, the continued low yields are an indication of insufficient inputs and/or inappropriate use of these technologies. The majority of the small-scale farmers are still aggravating the soil/plant nutrient deficit through improper land management and over-mining of the nutrient pool. However, there is still an opportunity to replenish the soil nutrient pool using integrated approaches depending on the degree of soil degradation, the production system and the type of nutrient in deficit.One potential source of organic fertilizer is farmyard manure. There is a large number of livestock in the Amhara region that could produce a considerable amount of manure to be used for soil fertility replenishment. However, there is a strong competition for manure use between soil fertility and its use as a cooking fuel. Recent survey in the upper central highlands of Ethiopia showed that more than 80% of the manure is used as a source of fuel. Only farmers with access to fuel wood could apply manure in their home steads. Experiences from Zimbabwe showed that most manures had very low nutrient content, N fertlizer equivalency values of less than 30%, sometimes with high initial quality that did not explain the quality of the manure at times of use (Murwira et al., 2002). This could be explained by the fact that most manures were not composed of pure dung but rather a mixture of dung and crop residues from the stall. Besides the quality the quantity of manure produced on-farm is limited. Sandford (1989) indicated that to produce sufficient manure for sustainable production of 1-3 tonnes/ha of maize it requires 10-40 ha of dry season grazing land and 3 to 10 of wet season Range land, which is beyond the capacity of Ethiopian farmers. Moreover, the potential of manure to sustain soil fertility status and productivity of crops is affected by the number and composition of animals, size and quality of the feed resources and manure management. Wet season manure has a higher nutrient content than dry season manure, and pit manure has a better quality than pilled manure. Similarly, Powell (1986) indicated that dry season manure had N-content of 6 g/kg compared with 18.9 g/kg for early rainy season manure when the feed quality is high.Another potential organic source is crop residue. Returning crop residue to the soil, especially of legume origin, could replenish soil nutrients, like nitrogen. However, there is strong tradeoff for use of crop residue between soil fertility, animal feed and cooking fuel. In the upper Ethiopian highlands crop residues are used as a major source for dry season feed and supplementary for wet season feed. Hence little is remaining as a crop aftermath to the soil. Although legumes are known to add nitrogen & improve soil fertility, the frequency of legumes in the crop sequence in the upper highlands is less than 10%, which implies that the probability of growing legume on the same land is only once in ten years. The most reliable option to replenish soil fertility is, therefore, promoting integration of multipurpose legumes into the farming systems. Those legumes, especially those refereed as legume cover crops, could produce up to 10 ton/ha dry matter within four months, and are also fixing up to 120 kg N per season (Giller, 2002). Those high quality legumes adapted to the Ethiopian highlands include tephrosia, mucuna, crotalaria, canavalia, and vetch (Amede & Kirkby, 2002). However, despite a significant after effect of LCCs on the preceeding maize yield (up to 500% yield gain over the local management) farmers were reluctant to adopt the legume technology because of trade-off effects for food, feed and soil fertility purposes (Amede, unpublished data, 2002). In an attempt to understand factors affecting integration of soil improving legumes in to the farming systems of southern Ethiopia, Amede & Kirkby (2002) identified the most important socio-economic criteria of farmers namely, land productivity, farm size, land ownership, access to market and need for livestock feed. By considering the decision-making criteria of farmers on which legumes to integrate into their temporal & spatial niches of the system, it was possible to integrate the technology to about 10% of the partner farmers in southern Ethiopia.Organic resources may provide multiple benefits through improving the structure of the soil, soil water holding capacity, biological activity of the soil and extended nutrient release, but it could be unwise to expect the organics to fulfil the plant demand for all basic nutrients. Most organic fertilizers contain very small quantities of some nutrients (e.g. P and Zn) to cover the full demand of the crop, and hence mineral fertiliser should supplement it. Combined application of organic fertilizers with small amount of mineral fertilizers was found to be promising route to improve the efficiency of mineral fertilizers in small holder farms. For instance, Nziguheba et al., (2002) indicated that organic resources enhanced the availability of P by a variety of mechanisms, including blocking of P-sorption sites and prevention of P fixation by stimulation of the microbial P uptake. Long term trials conducted in Kenya on organic and mineral fertiliser interaction also showed that maize grain yield was consistently higher for 20 years in plots fertilised with mineral NP combined with farmyard manure than plots with sole mineral NP or farmyard manure (S.M Nandwa, KARI, unpublished data 1997). Although most farmers are convinced of using farm-based organic fertilisers, they are challenged by questions like which organic residue is good for soil fertility, how to identify the quality of organic resource, how much to apply, when to apply, and what should be the ratio of organics to mineral fertilisers. This calls for development of decision support guides to support farmers' decision on resource allocation and management. Scientists from Tropical Soils Biology and Fertility Institute of CIAT developed decision guide to identify the quality of organic fertilisers based on the polyphenol, lignin and nutrient content as potential indicators (Palm et al., 1997). As those parameters demand laboratory facilities and intensive knowledge, Giller (2000) simplified the guide by translating it to local knowledge as highly astrigent test (high polyphenol content), fibrous leaves and stems (high lignin content) and green leaf colour (high N content) to make the guides usable to farmers.In general, there is an increasing trend of mineral fertilizer use in the Ethiopian highlands over the past decades, and fertilizer imports into the country have increased from 47000 tonnes N & P in 1993 to 137 000 tones in 1996 (Quinones et al., 1997). It was mainly as a result of a strong campaign of Sasakawa-Global 2000 in collaboration with the Buro of Agriculture. However, there is a declining trend in fertilisers use in 2001/2002 due to increasing cost of fertilizers, lack of credit opportunities to resource poor farmers and low income return due to market problems.Sustainable rural development and natural resource management in the region demands an investment in and improvement of the natural capital, human capital and social capital. As the natural capital in the region had multiple problems that needs multiple solutions, there is a strong need for holistic approach to deliver options for clients of various socio-economic categories.Given the complexity of the problem of land degradation, and its link to social, economical and policy dimensions, it requires a comprehensive approach that combines local and scientific knowledge through community participation, capacity building of the local actors through farmers participatory research and enhanced farmer innovation. This approach requires the full involvement of stakeholder at different levels to facilitate and integrate social, biophysical and policy components towards an improved natural resource management and sustainable livelihoods (Stroud, 2001). Watershed management as a unit of planning and change imposes the need for increased attention to issues of resource conservation and collective action by the community. The issues of land degradation may include afforstation of hillsides, water rehabilitation and/or harvesting and soil stabilization, soil fertility amendment through organic and mineral fertilizers and increasing vegetation cover by systematic use of the existing land and water resources. This could be achieved by working closely with communities and policy implementers in identifying and implementing possible solutions to address land degradation and other common landscape problems, like grazing land improvement, gully stabilization and by monitoring and documenting the processes for wider dissemination and coverage. Some of the watershed conservation related solutions should be tried and implemented on specific test locations using farmers' own contribution and the INRM team's technical supervision. However, a wider application of these solutions to larger areas may require attracting additional funding investments from the district, donors or other NGOs in the area. The local village communities may also effect changes in the norms and rules governing the use of natural resources in their vicinity. Traditional rules and local by-laws (e.g. written and unwritten and called \"afarsata\" or awatcheyache) regarding the use and sharing of resources exist in most villages and these need to be identified and studied with a view to effect reform or renew their emphasis in the community. Integration of Agroforestry technologies in the farming systems of the Ethiopian highlands failed because of absence of national and/or local policies /by-laws that prohibit free grazing and movement of animals in the dry season. Experiences from the 1980s campaign of 'Green Campaign' in Ethiopia also showed that it is almost impossible to address the issue of land degradation without the full involvement and commitment of the local community. The local by-laws in resource arrangement and use should be facilitated and supported, as the rules and regulations at the local level could be implemented effectively through elders and respected members of the community with tolerance and respect. There may be a church and/or witchcraft dimensions to these, and there may be changes over time that might help to understand why people are doing what they are doing. In addition, the influence of national and regional policies on local resource management should be understood. These will form an important subject of community wide discussion and deliberation (Stroud, 2001). The current undertaking of soil and water conservation practices through voluntary participation campaign of the community in the northern Ethiopian Highlands is one positive step forward for initiating collective action.The Sudano-Sahelian zone of West Africa (SSZWA) is the home of the world poorest people, 90% of whom live in villages and gain their livelihood from subsistence agriculture. Per capita food production has declined significantly over the past three decades. According to FAO, total food production in Sahelian countries grew by an impressive 70% from 1961 to 1996, but it lagged behind the population which doubled, causing food production per capita to decline approximately by 30% over the same period (Bationo, 1996).Low, erratic rainfall and high soil temperature, soil of poor native fertility, surface crusting and low water and nutrient holding capacity, and recurrent droughts are the main abiotic constraints to crop production in this environment.The table on economic and human development characteristics of West African countries indicate that except for Senegal, Côte d'Ivoire, Mauritania and Ghana where the percentage of undernourished people is less than 19%, most countries have between 20 to 34% of undernourished people and countries like Niger have more than 35% of their population undernourished. Sahelian countries produce 80% of their total cereal production under very difficult conditions. The ability to obtain the remaining 20 percent of required food is limited by low income and underdeveloped marketing channels. Gross domestic product per capita, for example, ranged from US$177 in Chad to US$575 in Senegal and have stagnated in real terms over the past decade. From the United Nations Development Programme's Human Development index, which ranks countries in terms of life expectancy, education and income, Sahelian countries fall in the bottom 15 percent of the 174 countries ranked, the lowest being Niger.In extensive agricultural systems, when crop yields decline to unacceptable levels, the land is left fallow to build up soil fertility, and new areas are then cultivated. Increasing population pressure has decreased the availability of land and resulted in reduced duration of fallow and increased the duration of cropping periods. Shifting cultivation is losing effectiveness and soil fertility is globally declining in many areas. The present farming systems are therefore unsustainable, low in productivity and destructive to the environment. Plant nutrient balances are negative (Stoorvogel and Smaling, 1990). The increasing need for cropland has prompted farmers to cultivate more and more marginal lands which are prone to erosion.Agricultural output should expand by at least 4% annually by the year 2000 in order to ensure food security. Previous studies have clearly shown that the expansion of new farms cannot increase output by over 1% without accelerating environment degradation. Consequently, productivity of land currently under cultivation should increase by at least 3% per annum. Presently, over a quarter of West African sub-region's population of two hundred million inhabitants is threatened by food insecurity. Any program aimed at reverting the declining trend in agricultural productivity and preserving the environment for present and future generations in West Africa must begin with soil fertility restoration and maintenance (Bationo et al., 1996).In this chapter, after a brief presentation of the crop production environment, we will present the state of the art of nitrogen, phosphorus and organic matter management for sustainable land use in the Sudano-Sahelian zone. Before presenting the new opportunities for future research for soil fertility restoration in this zone, we will discuss the effect of different cropping systems on soil fertility and also the main research achievements of the on-farm evaluation of soil fertility restoration technologies.The rainfall in West Africa shows a significant north-south gradient because of the inter-seasonal movement of the intertropical convergence zone, north and south of the equator. The rainfall is low, variable and undependable. The north-south rainfall gradient is very steep. The further one goes from the Sahara margins, the greater is the rainfall by approximately 1 mm km -1 . The isohyets run parallel (Toupet 1965). Sivakumar (1986) proposed a soil climatic zonation scheme for West Africa that is calculated from rainfall and potential evapo-transpiration. In this scheme a growing period of 60-100 days was used for defining the Sahelian zone. The geographical extent of the Sudanian zone has an average growing period of 100-150 days. The extent of the Sudano-Sahelian zone of West Africa (SSZWA) is represented by the Semi-Arid zone in Figure 1. The average annual rainfall of the cultivated zones varies from 300 to 900 mm and the ratio of annual rainfall to annual potential evapo-transpiration from 0.20 to 0.65. High soil temperature, sometimes exceeding 40 o C, can prevent crop establishment. Sand blasting and burial of the seedlings caused by wind erosion adds to this problem.Time dependent variations in rainfall are quite common in the region with coefficient of variation of annual rainfall ranges between 15-30%, and rainfall in some years can be 50% below or above the long-term average. In instance, Nicholson (1981) showed that in 1950 rainfall all over West Africa was above normal, at some location even 250% above normal. However, in 1970 rainfall was below normal throughout the region.It is well documented that precipitation determines the potential distribution of terrestrial vegetation and extended drought have initiated or exacerbated desertification. In the past 25 years, the SSZWA has experienced the most substantial decline in rainfall (Hulme and Kelly 1997;Hulme 1992;Nicholson and Palao 1993) and the downward trend is persistent since 1951 with more areas experiencing more higher rainfall variability. As a result of the decrease in rainfall there will be a decrease in the vegetation cover of the land and a reduction in the vegetation cover logically leads to reduce precipitation (Charney 1975;Cunnington and Rowntree 1986;Xue et al. 1990). The other non-climatic forces of desertification includes unsustainable agricultural practices, overgrazing and deforestation.With the reduction of the vegetation cover, the soil is left bare and therefore directly exposed to wind and water vegetation. The effect of these changes on wind and water erosion are aggravated by the sandy nature of the soils of SSZWA, which are frequently poorly aggregated, offering little resistance to the erosive forces. The Global Assessment of Soil Degradation (GLASOD) project estimates that 65% of the African agricultural land 31% of permanent pasture land, and 19% of forest and woodland has already been degraded. Three hundred and thirty two million hectares of African drylands are subjected to soil degradation. This represents one third of the entire area of dryland soil degradation in the world.Land degradation is one of the most serious threats to food production and soil lost through erosion is about 10 times greater than the rate of natural soil formation while deforestation is 30 times greater than of planned reforestation. Buerkert et al. (1996a) measured absolute soil lost of 190 t ha -1 in one year on bare plots, as opposed to soil deposition of 270 t ha -1 on plot with 2 t ha -1 millet stover mulch. Sterk et al. (1996) reported a total loss of 45.9 t ha -1 of soil during four consecutive storms. Buerkert et al. (1996b) reported that in unprotected plot up to 7 kg of available P and 180 kg ha -1 of organic carbon are lost from the soil profile within one year. Wind erosion will decrease also the exchangeable base and increase soil acidification. Wind erosion constitutes one of the major causes of land degradation. This results from the low vegetation cover at the time when the most erosive winds are blowing in combination with sandy, easy erodable soils. Wind erosion induced damage includes direct damage to crops through sand blasting, burial of seedling under sand deposits, and loss of top soils (Fryar 1971, Ambust 1984, Fryar 1990. The loss of the top soil which can contain 10 times more nutrients than the sub-soil is particularly worrying, since it potentially affects crop productivity on the long-term by removing the soil that is inherently rich in organic matter. b) Soils Entisols and Alfisols occupy most of the landscape in the SSZWA. Entisols are mainly composed of quartz sand, with low water and nutrient holding capacity. Alfisols have a clay accumulation horizon and a high base saturation because of lower rainfall and leaching but they have poor structural stability, poor water and nutrient holding capacity and lower organic matter than the ultisols and oxisols in the subhumid areas.The data in Table 1 shows physical and chemical properties of soils in the SSZWA. Most of the soils are sandy. One striking feature of these soils is their inherent low fertility which, is expressed in low levels of organic carbon (generally less than 0.3%), low total and available phosphorus and nitrogen and low effective cation exchange capacity (ECEC). The ECEC is attributed to low clay content and the kaolinitic mineralogy of the soils. Bationo and Mokwunye (1991) found that the ECEC is more related to the organic matter than to the clay content, indicating that a decrease in organic matter will decrease the ECEC and then the nutrient holding capacities of those soils. De Ridder and Van Keulen (1990) reported that a difference of 0.1% in organic carbon content results in a difference of 4.3 Cmol kg -1 in ECEC.Soil nutrient depletion is a major bottleneck to increased land productivity in the region and has largely contributed to poverty and food insecurity. Soil nutrient depletion occurs when nutrient inflows are less than outflows. Nutrient balances are negative for many cropping systems indicating that farmers are mining their soils. Table 2 shows the aggregated nutrient budgets for some West African countries. In Burkina Faso, current estimates indicate that in 1983, for a total of 6.7 million hectares of land cultivated, soil nutrient mining amounted to a total loss of 95000 tons of N, 28000 tons of P 2 O 5 and 79000 tons of K 2 O, equivalent to US$159 million of N, P and K fertilizers. In Mali, Van der Pol and Van der Geest (1993) reported that farmers extract, on average, 40% of their agricultural revenue from the soil mining. The significance of these figures is alarming when it is realized that productivity of these soils in their native state is already low because of low inherent levels of plant nutrients. The countries of the SSZWA consume less than 5 kg.ha -1 of plant nutrients and in addition there is intense pressure on the governments to remove subsidies on fertilizers without alternative policies to sustain even the current low levels of use of plant nutrients.The data in Table 3 indicates that continuous cultivation of the weakly buffered soils of northern Nigeria will result in a rapid decline of exchangeable cations and soil acidification in the Sudanian zone of Northern Nigeria. Soil calcium will decrease by 21% and pH by 4% after 50 years of continuous cultivation in farmers' fields.Rains in West Africa frequently occurs in short and intense storms and pose special problems in term of soil conservation (Kowal and Kassam 1978). Charreau (1974) reported on rainfall intensities between 27 to 62 mm h -1 . In Northern Nigeria, Kowal (1970) reported rainfall intensities over 250 mm h -1 for a short period. Hoogmoed reported a pick intensity of 300 mm hin Niono, Mali and a pick of 386 mm h -1 for Niamey, Niger (Hoomoed 1986). Land degradation due to water erosion is more severe in the Sudanian zone than in the Sahelian zone. On the bare, weakly crusted surface of the sandy Sahelian soil, infiltration rate of up to 100 mm h -1 have been reported (ICRISAT 1985). For the Alfisols with indurate crust, infiltration rates of 10.8 mm h -1 in Central Burkina Faso have been reported. As a result of the high rainfall intensities and low infiltration rates, runoff and soil loss are common in the region. The data in Table 5 indicate runoff and soil loss will depend on soil types and erodibility, land form and management system (Lal 1980). Whereas Sefa in Senegal with a slope of 1.2% on a bare soil a total runoff of 39.5% was recorded resulting in soil loss of 21 t ha -1 Yr -1 , in Burkina Faso with a slope of 1.20% only 7.5% of runoff was recorded with soil loss of 6.4 t yr -1 on pearl millet field.A) Nitrogen a) Introduction For many years, several scientists in the Sudano-Sahelian zones initiated research to 1) assess the performance of the different sources of N fertilizers 2) to assess the efficiency of different methods of N placement 3) to calculate 15 N balances in order to determine N uptake and losses and 4) to determine efficiency of N under different management systems and the effect of the different soil and agro climatic factors on the performance of N fertilizers (Mughogho et al. (1986), Bationo et al. (1989), Christianson and Vlek (1991), Ganry et al. (1973), Gigou et al. (1984)).Soil nitrogen is derived from air and dust, biological nitrogen fixation, organic sources, and fertilizers. About 98% of the soil nitrogen is stabilized in the organic matter. Thus the total nitrogen in the soil and the amount of nitrogen released for plant nutrients uptake will depend on organic matter content. b) Efficiency of N fertilizers as affected by N sources, methods of placement and time of application Christianson and Vlek (1991) used data from long-term experiment from the Sudano-Sahelian Zone to develop response function to N by pearl millet and sorghum and found that the optimum rate is 50 kg N/ha for sorghum and 30 kg N/ha for pearl millet. At these N rates the returns were 20 kg grain per kg N for sorghum and 9 kg grain per kg N for pearl millet.The use of 15 N in order to calculate N balances and to determine fertilizers N uptake and losses provide an important tool for nitrogen management. Results with 15 N research in early years are reported in Mughogho et al. from which the following conclusion can be made. 1) Apparent uptake of fertilizer N exceeds measured uptake using 15 N. 2) Uptake of 15 N labelled fertilizer and apparent recovery of unlabelled N decreases with increasing rates of application. 3) Loss of 15 N labelled fertilizer to the atmosphere and recovery of 15 N in the soil increases with increasing rates of fertilizer application. 4) Estimated losses of N are high regardless of N sources.The urea and calcium ammonium nitrate (CAN) are the most common sources of nitrogen in the region. Trials were undertaken to evaluate these two sources of nitrogen with basal or split application, banded, broadcast or applied point placed as urea supergranule (USG) or CAN point placed. 15 N was applied in microplot in order to construct N balances and to determine N uptake and losses from the different sources of N, methods of application and timing of application.From the data in table 5,6 and 7 the following conclusion can be made: 1) Fertilizer N recovery by plant was very low, averaging 25 -30% over all years. 2) There is a higher loss of N with the point placement of urea (USG) (> 50%) and the mechanism of N loss is believed to have been ammonia volatilization. 3) For all years losses of N from CAN were less than from urea because one-half of the N in CAN is in the non-volatile nitrate form. 4) Although CAN has a lower N content than urea, it is attractive as an N source because of its low potential for N loss via volatilization, and its point placement will improve its spatial availability. The data in Figure 2 clearly indicates that CAN point placed outperformed urea point placed or broadcast and 15 N similar trials indicate that 15 N uptake by plants was almost three times higher from CAN than that of urea applied in the same manner (Table 7). c) Efficiency of N fertilizers as affected by soil and crop management and rainfall Mughogho et al. (1986) found significant relationships between crop yields and N recovery. N losses averaged 20% in the humid and sub-humid zones with maize and were significantly less than the average loss of 40% found over all treatments in the Sudano-Sahelian zone.In the Sahelian zone, Bationo and Vlek (1998) reported nitrogen use efficiencies of 14% in plots without lime and phosphorus whereas this amount increased to 28% when P and lime were applied.Rotation of cereals with legumes could be a way to increase N use efficiency. Bationo and Vlek 1998 reported a nitrogen use efficiency of 20% in the continuous cultivation of pearl millet but its value increased to 28% when pearl millet was rotated with cowpea. Bationo et al. (1989) found a strong effect between planting density and response to N fertilizer. Christianson et al. (1990) developed a model on the effect of rainfall on N for pearl millet production in the Sahel and found that the response to N was affected by rainfall over a 45 days yield-sensitive period which coincides with the culms elongation and anthesis growth stages for millet (Figure 3).(2) Phosphorus sources and management a) Introduction Among soil fertility factors, phosphorus deficiency is a major constraint to crop production in the Sudano-Sahelian zone. For many years, research has been undertaken to assess the extent of soil phosphorus deficiency, to estimate phosphorus requirement of major crops, and to evaluate the agronomic potential of various phosphate rock (PR) from local deposits (Goldsworthy, 1967;Pichot and Roche, 1972;Thibout et al. 1980;Bationo et al. 1987;Bationo et al. 1990;Hauck, 1966;Jones, 1973;Juo and Fox, 1977;Kang and Osiname, 1979;Boyer, 1954;Nalos et al. 1974;Juo and Kang, 1978;Mokwunye, 1979;Truong et al. 1978) About 80% of the soils in sub-Saharan Africa are short of this critical nutrient element and without the use of phosphorus, other inputs and technologies are not effective. However, sub-Sahara Africa use 1.6 kg P/ha -1 of cultivated land as compared to 7.9 and 14.9 respectively for Latin America and Asia. It is now accepted that the replenishment of soil capital phosphorus is not only a crop production issue, but an environmental issue and P application is essential for the conservation of the natural resource base.Availability and total P levels of soil are very low in the SSZWA as compared to the other soils in West Africa (Bache and Rogers, 1970;Mokwunye, 1974;Jones and Wild, 1975;Juo and Fox, 1977). For the sandy Sahelian soils total P values can be as low as 40 mg P kg -1 and the value of available P less than 2 mg P kg -1 . In a study of the fertility status of selected pearl millet producing soils of West Africa, Manu et al. 1991 found that the amount of total P in these soils ranged from 25 to 340% mg kg -1 with a mean of 109% mg kg -1 . The low content of both total and available P parameters may be related to several factors including 1) Parent materials, which are mainly composed of eolian sands, contain low mineral reserves and lack primary minerals necessary for nutrient recharge. 2) A high proportion of total P in these soils is often in occluded form and is not available to crop (Charreau, 1974). 3) Low level of organic matter and the removal of crop residue from fields. Organic matter has a favourable effect on P dynamics of the soil; in addition to P release by mineralization, the competition of organic ligands for Fe and Al oxides surface can result in a decrease of P fixation of applied and native P.The P sorption characteristics of different soil types has been investigated and as compared to the soils of the more humid regions, the soils of the SSZWA have very low capacity to fix P (Sanchez and Uehara, 1980;Udo and Ogunwale, 1972;Fox and Kamprah, 1970;Juo and Fox, 1977;Syers et al. 1971). For pearl millet producing soils, Manu et al. 1991 fitted the sorption data to Langmuir equation (Langmuir 1918) and P sorption maximum was determined using the method of Fox and Kamprath, 1970. From these representative sites in the Sudano-Sahelian zone the values of maximum P sorbed ranged from 27 mg kg -1 to 253 mg kg-1 with a mean of 94 mg kg -1 .Phosphorus deficiency is a major constraint to crop production and response to nitrogen is substantial only when both moisture and phosphorus are not limiting. Field trials were established to determine the relative importance of N, P and K fertilizers. The data in Table 8 indicates that from 1982 to 1986 the average control plot was 190 kg grain ha -1 . The sole addition of 30 kg P2O5/ha without N fertilizers increased the average yield to 714 kg ha -1 . The addition of only 60 kg N/ha did not increase the yield significantly over the control and the average grain yield obtained was 283 kg ha -1 . Those data clearly indicate that P is the most limiting factor in those sandy Sahelian soils and there is no significant response to N without correcting first for P deficiency. When P is applied the response to N can be substantial and with the application of 120 kg N ha-1 a pearly millet grain yield of 1173 kg ha-1 was obtained as compared to 714 kg ha-1 when only P fertilizers were applied. For all the years the addition of potassium did not increase significantly the yield of both grain and total dry matter of pearl millet.b) The use of alternative locally available phosphate rock Despite the fact that deficiency of P is acute on the soils of West Africa, very little P fertilizers is used by local farmers, partially because of the high cost of the imported fertilizers. The use of locally available phosphate rock indigenous in the region could be an alternative to use of high cost imported P fertilizers. The effectiveness of phosphate rock (PR) depends on its chemical and mineralogical composition (Khasawneh and Doll (1978), Lehr and McClellan (1972), Chien and Hammond (1978). The most important feature of the empirical formula of francolite is the ability of carbonate ions to substitute for phosphate in the apatite latice. Smith and Lehr (1966) concluded from their studies that the level of isomorphic substitution of carbonate for phosphate within the latice of the apatite crystal influences the solubility of the apatite in the rock and therefore controls the amount of phosphorus that is released when PR is applied to soil. The most reactive PR are those having a molar PO 4 /CO 3 ratio less than 5. West African PR's are not very reactive. Chien (1977) found that the solubility of PR in neutral ammonium citrate (NAC) was directly related to the level of carbonate substitution. Diamond (1979) proposed a classification of phosphate rock for direct application based on citrate solubility as >5.4% high; 3.2-4.5% medium and <2.7% low. Based on this classification only Tilemsi PR has a medium reactivity.Environmental conditions, crop types, and management practices control the P supply and hence the effectiveness of a given PR in a given crop management environment, (Mokwunye, 1995). The ability of the soil to provide H+, soil with low P and Ca, soil moisture, the acidification of the rhizosphere, plants with high root density and high Ca uptake play an important role in P availability from PR (Kasawneh and Doll, 1978;Chien, 1977;Mokwunye, 1995;Hammond et al. 1986;Kirk and Nye, 1986;Hedley et al. 1982;Sale and Mokwunye, 1993;Föhse et al. 1988;Barrow, 1990;Hammond et al. 1989). Bationo et al. (1987) have shown that direct application of PR indigenous to the region may be an economical alternative to the use of more expensive imported water-soluble P fertilizers for certain crops and soils. Bationo et al. 1987 while evaluating Parc-W and Tahoua PR indigenous to Niger found that PR is only 48% as effective as single superphosphate (SSP), whereas the effectiveness of the more reactive Tahoua rock was as high as 76% of SSP. Further studies by Bationo et al. (1990) showed that Tahoua PR is suitable for direct application, but Parc-W has less potential for direct application. The data from a long-term benchmark experiment show that SSP outperformed the other sources and its superiority to sulphur-free Triple Superphosphate (TSP) indicates that with continuous cultivation, sulphur deficiency develops. For both pearl millet grain and total dry matter yields, the relative agronomic effectiveness was almost similar for TSP as compared to the partially acidulated Parc W phosphate rock (PAPR) with 50% acidulation (PAPR50) indicating that partial acidification of Parc-W PR can significantly increase its effectiveness (Bationo et al 1996).In trials conducted in the different agro-ecological zones of Niger it was found that Tahoua PR outperformed Kodjari PR (from Burkina Faso) (Figure 4). The results are in agreement with the fact that the molar PO 4 /CO 3 ratio is 23 for Kodjari PR and 4.9 for Tahoua PR, and Tahoua PR has also a higher solubility in NAC. Bationo et al. (1997) found that Tilemsi PR can result in net returns and value/cost ratios similar to recommended cotton or cereal complex imported fertilizers.There is ample evidence that indicates that market differences exist between species and genotype for P uptake (Föhse et al. 1988;Caradus, 1980;Nielsen and Schjorring, 1983;Spencer et al. 1980). Bationo et al. 2001 found that the PUE among nine pearl millet varieties varied from 25 kg grain/kg P for variety ICMVIS 85333 to 77 kg grain/kg P for Haini-Kirei cultivar.The data on Table 9 clearly shows that hill placement of small qualities of P fertilizers will have a higher phosphorus use efficiency (PUE) as compared to the broadcasting of 13 kg P/ha as recommended by the extension services. Whereas in 1995 the PUE was 47 with the broadcasting of 13 kg/P ha, a value of 111 was obtained with the hill placement of 3 kg P/ha with the seed at sowing time. In on-farm researchers managed trials in the Sahelian zone, it was found that the efficiency of PR from Kodjari or Tahoua can be improved with the hill placement of 4 kg P/ha. Whereas the PUE of Kodjari PR applied alone was 14 it increased to 31 when additional P was hill placed at seedling time as 15-15-15 for pearl millet grain yield (Bationo, unpublished data).In long-term soil management trials, application of nitrogen, crop residue and ridging and rotation of pearl millet with cowpea were evaluated to determine their effect on PUE. The results show that soil productivity of the sandy soils can dramatically increase with the adoption of improved crop and soil management technologies, whereas the absolute control recorded 33 kg ha -1 of pearl millet grains, 1829 kg ha -1 was obtained when phosphorus nitrogen and crop residue was applied to the ridged and fallowed leguminous cowpea upon the previous season. Results indicate for the grain yield that PUE increases from 46 with only P application to 133 when P is applied in combination with nitrogen, crop residue and the crop is planted on ridge in a rotation system (Table 10). D. Organic matter management a) Introduction Maintaining soil organic matter is a key to sustainable land use management. Organic matter acts as source and sink for plant nutrients. Other important benefits resulting from the maintenance of organic matter is low-input agro-systems include retention and storage of nutrients, increasing buffering capacity in low activity clay soils, and increasing water holding capacity. Nye and Greenland (1960) estimated that the annual increase in nitrogen under forest fallow was 30 kg N ha -1 in the soil and 60 kg N ha -1 in the vegetation. For the savanna ecosystems, the annual increase was 10 kg N ha -1 in the soil and 25 kg N ha -1 in leaves and vegetation. Bationo et al. 1995 reported that continuous cultivation in the Sahelian zone has led to drastic reduction in organic matter and a subsequent soil acidification. Bationo and Mokwunye (1991) reported that in the Sudano-Sahelian zone, the effective cation exchange capacity (ECEC) is more related to organic matter than to clay, indicating that a decrease in organic matter will decrease the ECEC and subsequently the nutrient holding capacity of these soils. In a study to quantify the effects of changes in organic carbon on cation exchange capacity (CEC) De Ridder and Van Keulen (1990) found that a difference of 1 g kg-1 in organic carbon results in a difference of 4.3 mol kg -1 . In many cropping systems few if any agricultural residues are returned to the soil. This leads to decline soil organic matter, which frequently results in lower crop yields or soil productivity.The concentration of organic carbon in the top soil is reported to average 12 mg kg -1 for the forest zone, 7 mg kg -1 for the Guinean zone, 4 mg kg-1 in the Sudanian zone and 2 mg kg -1 for the Sahelian zone (Windmeijer and Andriesse (1993). The soils of the Sudano-Sahelian zone are inherently low in organic carbon. This is due to the low root growth of crops and natural vegetation but also the rapid turnover rates of organic materials with high soil temperature and microfauna, particularly termites. In a survey of millet producing soils, Manu et al. (1991) found an average soil Corg content of 7.6 g kg -1 with a range from 0.8 to 29.4 g kg -1 . The data also showed that these Corg contents were highly correlated with total N (R = 0.97) which indicates that in the predominant agro-pastoral systems without the application of mineral N fertilizers, N nutrition of crops largely depend on the maintenance of soil Corg levels.The importance of soil textural (clay and silt) properties for the Corg content of soil was stressed repeatedly as clay is an important component in the stabilization of organic molecules and minoorganisms (Amato and Ladd, 1992;Greenland and Nye, 1959;Feller et al. 1992). Thus Feller et al. (1992) reported that independently of climatic variations such as precipitation, temperature, and duration of the dry seasons Corg increased between 600 and 3000 mm annual rainfall with the clay and silt contents of low activity clay soils. Therefore small variations in topsoil texture at the field or watershed level could have large effects on Corg. b) Effect of soil management practices on organic carbon contents There is much evidence for rapid decline of Corg levels with continuous cultivation of crops in the SSZWA (Bationo et al. 1995). For the sandy soils, average annual losses in Corg often expressed by the K value (calculated as the percentage of organic carbon loss per year), may be as higher as 4.7%, whereas for the sandy loam soils, reported losses seem much lower, with an average of 2% (Pieri, 1989, Table 11). The data in Table 11 also clearly indicated that soil erosion can increase Corg losses from 2% to 6.3% and management practices such as crop rotation, following soil tillage, application of mineral fertilizers and mulching will have a significant effect on annual losses of Corg. The K-value in cotton cereal rotations were 2.8%, lower than the 2.8%, lower than the 2.8% in continuous cotton system. At Nioro-du-Rip in Senegal, soil tillage increased annual Corg losses from 3.8% to 5.2% and annual Corg losses declined from 5.2% without NPK to 3.9% with NPK application. c) Effects of crop residues and manure on soil productivityThe Sahelian zone In long-term crop residue and management trials, Bationo and Buerkert 2001 reported for the Sahelian zone a very significant effect between crop residue and mineral fertilizer (Figure 5). From this experiment started since 1984 Bationo et al. (1993) reported that the grain yield declined to 160 kg ha -1 in unmulched and unfertilized plots. However, grain yield could be increased to 770 kg ha -1 with a mulch of 2 t crop residue per hectare and 1030 kg ha -1 with 13 kg P plus 30 kg N ha -1 . The combination of crop residue and mineral fertilizers resulted in grain yield of 1940 kg ha -1 . The application of 4 t of crop residue per hectare maintained soil organic carbon at the same level that in an adjacent fallow field in the top soil but continuous cultivation without mulching results in drastic reduction of Corg (Figure 6). In the Sudanian zone, all available reports show a much smaller or even negative effect of crop residue use as soil amendment (Bationo et al. 1995;Sedogo, 1993). In the Sahelian zone the application of crop residue increased soil pH, and exchangeable bases and decrease the capacity of the soil to fix phosphorus.On the nutrient poor West African soil, manure, the second farm-available soil amendment can substantially enhance crop yields. For Niger, McIntire et al. (1992) reported grain yield increase between 15 and 86 kg for millet and between 14 and 27 kg for groundnut per ton of applied manure. Similar manure effects have been reported from other Sahelian countries. However, given the large variation in the nutrient concentration of the manure types applied comparisons between results from different experiments should be made with precaution. Powell et al. (1998) a very significant effect of manure and urine application on pearl millet in the Sahelian zone.In the SSZWA crop residues use as surface mulch can plan an important role in the maintenance of Corg levels and productivity of the prevailing acid soils through the recycling of mineral nutrients, increased in fertilizer use efficiency and a decrease in soil erosion effect. However, organic material available for surface mulching are scarce given the low overall production levels of biomass and their multiple competitive use as fodder, construction material and cooking fuel (Lamers and Feil, 1993). The crop residue quantities found on-farm at the beginning of the rainy season ranged from 0 to 500 kg ha -1 . McIntire and Fussel (1986) reported that on farmers' fields in the Sahel average grain yields were 236 kg ha -1 and mean crop residue yields barely reached 1300 kg ha -1 . Baidu-Forson (1995) reported on availability of 250 kg ha -1 of crop residue at the onset of the rains. Powell et al. (1987) showed that 50% of the disappearance rates of millet stover could be attributed to livestock grazing.Animal manure has a similar role as residue mulching for the maintenance of soil productivity but depending on rangeland productivity, it will require between 10 to 40 ha of dry season grazing land and 3 to 10 ha of rangeland of wet season grazing to maintain yields one one hectare of cropland (Fernandez-Rivera et al. 1995). The potential of manure to maintain soil Corg and sustain crop production is thus limited by the number of animals available and the size and quality of the rangeland.At the farm level, the maintenance of Corg levels in the soils of the region will largely depend on an increase in C fixation by plants. Given the strong limitation of plant growth by the low availability of mineral nutrients, a yield-effective application of mineral fertilizers is crucial. It would not only allow large increase in crop production and the amount of by-products but also to improve soil coverage by forage grass and weeds. D) Relationships between cropping systems and fertility management a) Introduction The most common cropping system involves growing several crops in association as mixtures or intercrop. This practice provides the farmers with several options for returns from land and labor, often increases efficiency with scarce resources, and reduces dependence upon a single crop that is susceptible to environment and economic fluctuations. Steiner (1984) reported that traditional intercropping systems cover 75% of the cultivated land in the SSZWA. The principal reasons for farmers to intercrop are flexibility, profit and resource maximization, risk minimization, soil conservation and maintenance, weed control and nutritional advantages (Norman, 1974;Swinton et al. 1974;Fusel and Serafini, 1985).Cowpea (Vigna unguiculata (L.) Walp) and groundnut (Arachis hypogea L.) are two of the predominant grain legumes in the SSZWA. Groundnut occupies 2.7 million hectares of arable land and cowpea 6 million hectares. The two legumes are important components of the mixed cropping systems of the resource-poor farmers. The most important cereals are sorghum and pearl millet and the two legumes are often intercropped with these cereals. While considerable information is available on fertilizer requirements for sole cropping of various crops, little is known on fertilizer requirement in intercropping.In the mixed cropping systems, legume yields are very low due to low soil fertility, low planting densities and pest and decrease (Ntare,1989;Reddy et al. 1992). The yield of cowpea grain varies between 50 and 300 kg ha -1 in farmers fields in marked contrast to yield over 2000 kg ha -1 obtainable on research station and by large scale commercial enterprises in pure cropping. Rotation of cereals with legumes has been extensively studied in recent years. The use of rotational systems involving legumes for harvesting nitrogen fixation is gaining importance throughout the region because of economic and sustainability considerations. The beneficial effect of legumes on succeeding crops is normally exclusively attributed to the increased soil N fertility as a result of N-fixation. The amount of N2 fixed by leguminous crops can be quite high but some workers have demonstrated also that legumes can deplete soil nitrogen (Rupela and Saxena, 1987;Blumenthal et al. 1982;Tanaka et al. 1983). Most data reported on the quantity of N fixed by the legume crops in the SSZWA concerned the above ground part of the legume and very little is known on the nitrogen fixed by roots where much of the legume bio-mass is returned to the soil as green manure a positive N balance is to be expected. However, this may not be true for grain legumes and fodder. Where the bulk of above legume material is removed from the system. Nevertheless, many other positive effects of grain legumes such as the improvement of soil biological and physical properties and the ability of some legumes bounded phosphorus by roots exudates (Gardner et al. 1991;Arihara and Okwaki, 1989).Other advantages of crop rotations include soil conservation (Stoop and Staveren, 1981) organic matter restoration (Spurgeon and Grimson, 1965) and pest and disease control (Sunnadurai, 1973).In the mixed crop-livestock systems of the SSZWA, increasing legume component in the farming systems is important in order to increase the availability of fodder as source of livestock feed while increasing soil fertility. b) Intercropping Fussel and Serafini (1985) reported yield advantages from 10-100% in millet cowpea systems.Yield stability has been proposed as a major advantage of intercropping (Wiley, 1979a(Wiley, , 1979b;;Willey et al. 1985;Steiner, 1984) as farmers want to rely on management practices that increase yields, when this is possible, while improving the stability of the production in both good and poor rainfall years. Baker (1980) has compared relative stability of intercropping and cropping using stability analysis of Finlay and Wilkinson (1963) and found that in the groundnut/cereal systems in northern Nigeria, intercropping systems were found to be more stable. Ntare (1989) reported yield advantages of 20-70% depending on the different combinations of pearl millet and cowpea cultivars. Although traditional intercropping cover over 75% of the cultivated area in the SSZWA, there is a scarcity of information on the efficiency of fertilizers under these systems. The number of days before planting the second crop will depend on the importance of the next rains after the first cereal crops have been planted. With a basal application of P fertilizers the cereal growth is rapid and can suppress completely the second crop if its planting occurs after three weeks after the cereal crops have been sown. In contrast if the legume crops is planted early it will compete more with the cereal crop for light, water and nutrients and can significantly reduce the yield of the cereal crop. c) Relay and sequential cropping In the Sudanian zone with longer growing season and higher rainfall there is greater opportunity than in the Sahelian zone to manipulate the systems with appropriate genotypes and management systems. Field trials have been conducted to examine the performance of the cultivars under relay and sequential systems and revealed the potential of these alternative systems over traditional sole or mixed cropping (ICRISAT, 1984 and1987).In Mali, by introducing short season sorghum cultivars in relay cropping with other short duration cowpea and groundnut cultivars, substantial yields of legumes and sorghum were obtained as compared to traditional systems (IER, 1990;Sogodogo and Shetty, 1991).In the Sahelian zone (Sivakumar, 1986) analysed the data of the onset and ending of the rains and the length of the growing period. He found that an early onset of the rains offers the probability of a longer growing period while delayed onset results in a considerable short term growing season. The above analysis suggests that even for the Sahelian zone, cropping management factors using relay cropping can increase soil productivity with an early onset of the rains. d) Crop rotation Despite the recognised need to apply chemical fertilizers for high yields, the use of fertilizers in West Africa is limited by lack of capital, inefficient distribution systems, poor enabling policies and other socio-economic factors. Cheaper means of improving soil fertility and productivity are therefore necessary.Cereals and legumes rotation effects on cereals yields have been reported by several scientists (Bationo et al. 1998;Klaij and Ntare, 1985;Stoop and Van Staven, 1981). Bationo and Ntare, 2000 data at Tara in the Sudanian zone clearly indicates that at all levels of nitrogen application the yield of pearl millet after cowpea outperformed the yield of millet in the continuous millet cultivation.N has been used to quantify the amounts of nitrogen fixed by cowpea and groundnut under different soil fertility levels. The nitrogen derived from the air (NDFA) varies from 65 to 88% for cowpea whereas the values varied from 20 to 75% for groundnut. In the complete treatment where all nutrients were applied cowpea stover fixed up to 89 kg N ha -1 whereas for same treatment groundnut fixed only 40 kg N ha -1 in this Sahelian environment (Bationo and Vlek 1990). In order to determine 15 N recovery from different cropping systems, labelled nitrogen fertilizers were applied to microplots of pearl millet grown continuously, in rotation with cowpea, in rotation with groundnut, intercropped with cowpea, and intercropped with groundnut. The data indicates that nitrogen use efficiency increased from 20% in continuous pearl millet cultivation to 28% when pearl millet was rotated with cowpea (Bationo and Vlek, 1998). The same authors reported that in the Sudanian zone nitrogen derived from the soil increased from 39 kg N ha-1 in continuous pearl millet cultivation to 62 kg N/ha when pearl millet is rotated with groundnut. Those data clearly indicate that although all the above ground biomass of the legume will be used to feed livestock and not returned to the soil, rotation will increase not only the yields of succeeding cereal crop but also its nitrogen use efficiency. Bayayoko et al. (2000) in studies of cereals legumes effects on cereal growth in the Sudano-Sahelian zone of West Africa reported that the rotation effect although significant in most of the cases varied with sites and years. At Sadore as an example, the millet rotated with cowpea yielded 1904 kg/ha whereas the continuous millet cultivation yielded 1557 kg ha-1. Bayoyoko et al. 2000 reported higher levels of mineral N and native arbuscular mychorrhizae infection in the rotation systems as compared to the continuous cereal cultivation.The different cropping systems have a significant effect on the soil organic carbon. The soil organic carbon levels was 0.22% in the continuous systems whereas it is increased to 0.27% in the rotation systems. As a result of this soil pH was higher in the rotation systems as compared to the continuous monoculture (Bationo, unpublished data).A review of the state of the art of the agronomic research in soil fertility management showed that onstation research has developed a considerable amount of promising results but very few of these technologies have reached the small farmers. It is recognized that most of these technologies developed on-station are not always built on indigenous practices, local socio-economic realities, farmers priorities and perceptions. Most often no account has been taken of enabling policy environment and indigenous knowledge. Therefore on-farm research should involve farmers, researchers, extension agents, nongovernmental agencies at the design, implementation and evaluation stages. In this way, the technologies generated have a better chance of adoption by the land users. Promising technologies were identified to be tested on-farm under farmers managed trials knowing that a particular farm management practice is often less effective in the hand of the farmer, than it is on-station. There is need for experimental farm input packages to be tested under farmer's conditions to allow the scientists to observe the transfer of technologies to the farmers field and to determine associated management practices to be adopted by farmers in order to ensure good economic returns.The objectives of on-farm research activities is: 1) to assess farmers' perception of the different technologies proposed 2) to identify the farmers' management practices affecting the good performance of the different technologies 3) to evaluate the profitability of the different technologies tested 4) to identify the constraints to technology adoption and means to alleviate them and 5) to assess the impact of technology adoption. b) Effects of soil fertility restoration technologies on land productivity from farmers managed trials in the SSZWA In the Sahelian zone of Gobery in Western Niger, 20 farmers evaluated phosphorus and nitrogen fertilizers including partially acidulated phosphate rock (PAPR) from parc-W. The data in Table 12 indicate a strongly response to P with yield increase of 181% over control with the application of N and P. No significant difference was found between PAPR and SSP, nor was difference between broadcasting and hill placement of nitrogen. However, crop response to fertilizer use was strongly affected by the cropping density chosen by individual farmers (Bationo et al. 1992). Averaged over all fertilized treatments and all years, when farmers planted at less than 3500 pockets per hectare, yield was very low and no response was found to fertilizer use. However, each 1500 pocket/ha increases about 200 kg grain/ha. Bationo and Baidu-Forson (1998) reported the agro-economic evaluation of farmers managed trials on the evaluation of water soluble fertilizers, phosphate rock and rotation of cereals with legumes. The net grains, over three years, resulting from partial budgeting analysis show that farmers could make net financial gains with only the application of P fertilizer. The use of N in addition to P significantly improved net grains. Water soluble single superphosphate generated higher net gains than Tahoua phosphate rock. As a result of the higher cowpea price and is beneficial effect on the improvement of soil fertility, the rotation systems involving cowpea were more profitable than continuous pearl millet cultivation. Bationo et al. 1997 reported the economic evaluation data of Tilemsi phosphate rock by farmers in three agro-ecological zones of Mali. The agro-ecological zones were Tafla with an average rainfall of 600 mm, Sougoumba with 800 mm rainfall and Tinfounga with 1200 mm rainfall. The cropping systems used were rotation of pearl millet with groundnut in Tafla, sorghum with cotton in Sougoumba and maize with cotton in Tinfounga. The data indicate that the different sources of fertilizers have a significant effect on crop yields and there was no difference between Tilemsi phosphate rock and the recommended imported water soluble fertilizers. However, the economic analysis of the data indicate that at some sites the imported recommended water soluble P fertilizers are more profitable than the use of Tilemsi phosphate rock. Bationo et al. 1998 undertook an agro-economic evaluation of a set of soil fertility restoration technologies and concluded that hill placement of small quantities of P fertilizers at planting time had higher returns than broadcasting 13 kg P/ha.From 1988 to 2000 farmers-managed trials in the Sahelian zone at Karabedji (~550 mm of rainfall per year) and in the Sudanian zone at Gaya (~800 mm of rainfall per year), over an average of about 2800 field plots showed the agronomic potential of fertilizers (Table 13). The hill placement of 4 kg P/ha almost doubled crop yield. Integrated use of hill placement of water soluble fertilizers in addition to Tahoua phosphate rock broadcast and soil amendment with crop residue application as mulch gave the highest crop yield (Figure 7). The returns over variable cost of fertilizers presented clearly demonstrate the economic importance of soil fertility restoration in the SSZWA.a) New strategies for integrated nutrient management In the past, integrated nutrient management concentrated mainly on the utilization of available organic and inorganic sources of plant nutrients in a judicious and efficient way. Integrated nutrient management is recently perceived much more broadly as the judicious manipulation of all soil nutrient inputs and outputs and internal flows.Future research needs to adopt this new holistic approach to integrated nutrient management. For a given cropping system or watershed, this will require the establishment of the nutrient balances. Interventions to limit nutrient losses through erosion can be in some cases as important as research on increasing the efficiency of organic and inorganic plant nutrients for a sustainable land use. This new approach will enhance more carbon sequestration and increase more bio-mass production on the farms for domestic use and there will be more bio-man available for livestock feeds and for soil mulching. b) Integration of socio-economic and policy research with the technical solution In the past several technical solutions to the problem of land degradation in the SSZWA have been researched and tested, and may have shown the potential for addressing the problem in some places. Unfortunately a review of the state of the art indicated that very few of these technologies have been adopted by the resource poor farmers. Therefore future research should focus more on problems driven by socio-economic factors and enabling policy environment in order to enhance farmers' capacity to invest in soil fertility restoration. The adoption of the participatory approach will be essential. In this way, the technologies generated have a better chance of adoption by land users. c) Combining rain water and nutrient management strategies to increase crop production and prevent land degradation In the SSZWA high inter-annual variability and erratic rainfall distribution in space and time result in water-limiting conditions during the cropping season. In areas with inadequate rainfall or in runoff-susceptible land, water conservation techniques and water harvesting techniques offer the potential to secure agricultural production and reduce the financial risks associated with the use of purchased fertilizers. Under the conditions of adequate water supply, the addition of organic and inorganic amendments is the single most effective means of increasing water use efficiency. Future research needs to focus on enhancing rainwater and nutrient use efficiencies and on capitalizing on their synergies for increasing crop production and preventing soil degradation. d) Increasing the legume component for a better integration of crop-livestock production systems The rotations of cereals with legumes have led to increased cereals yield at many locations in the SSZWA. Factors such as mineral nitrogen increase, enhancement of Vesicular-Azbuscular Mycorrhizal (VAM) for better P nutrition and a decrease in parasitic nematodes have been identified as mechanisms accelerating the enhanced yield of cereals in rotation with legumes. Most of the research quantify has focused on the quantification of the above-ground N fixed by different legumes cultivars, but very little is known on the below-ground N fixed.There is need to increase the legume component in the mixed cropping systems for a better integration of crop-livestock. The increase of legume component in the present cropping system will not only improve the soil conditions for the succeeding cereal crop, but will provide good quality livestock feed, and the manure produced will be of better quality for soil amendment. e) Exploiting genetic variation for nutrient use efficiency Phosphorus is the most limiting plant nutrient for crop production in the SSZWA and there is ample evidence that indicates marked differences between crop genotypes for P uptake. A better understanding of the factors affecting P uptake such as the ability of plants to i) solubilize soil P through acidification of the rhizosphere and the release of chelating agents and phosphate enzymes ii) explore a large volume of soil and iii) absorb P from low P solution would help screening for the genotypes the best appropriate for nutrient use efficiency.Another important future research opportunity is the selection of genotypes that can efficiently associate with Vesicular-Azbuscular Mycorrhizal (VAM) for better utilization of P applied as indigenous phosphate rock. f) Use of decision support systems modelling, and GIS for the extrapolation of research findings Farmers production systems vary with respect to rainfall, soil types and socio-economic circumstances and therefore they are complex. Dealing with such complexity only by empirical research will be expensive and inefficient. Use of models and GIS will facilitate the transfer of workable technologies to similar agro-ecological zones. The use of DSSAT, APSIM and GIS will facilitate extrapolation of findings to other agro-ecozones similar of the benchmark sites chosen for testing technologies and will be cost effective. agricultural productivity and preserving the environment for present and future generations in West Africa must begin with soil fertility restoration and maintenance. Cowpea (Vigna unguiculata (L.) Walp) and groundnut (Arachis Hypogea L.) are two of the predominant grain legumes in the semi-arid tropics of West Africa. Groundnut occupies 2.7 million hectares of arable land and cowpea 6 million hectares. The two legumes are important components of the mixed cropping systems of resource poor farmers. The most important cereals are sorghum and pearl millet and legumes are often intercropped with these cereals (Steiner 1984).The common cropping system involves growing several crops in association as mixtures or intercrop. This practices provide the farmers with several options for returns from land and labor, often increase efficiency with which scarce resources are used, and reduce dependence upon crop that is susceptible to environmental and economic fluctuations. While considerable information is available on fertilizer requirements for sole cropping of various crops, little is known on fertilizer requirement for inter-cropping. Steiner (1984) reported that traditional intercropping systems cover 75% of the cultivated area in the WASAT. The principal reason for farmers to intercrop are flexibility, profit resource maximization, risk minimization, soil conservation and maintenance, weed control and nutritional advantages (Norman 1974;Swinton et al. 1974;Shetty et al. 1995;Fussel and Serafini 1985).Rotation of cereals with legumes has been extensively studied in recent years. Use of rotational systems involving legumes for harvesting nitrogen fixation is gaining importance throughout the region because of economic and sustainability considerations. The beneficial effect of legumes on succeeding crops is normally exclusively attributed to the increased soil N fertility as a result of N 2 -fixation. The amount of N 2 fixed by leguminous crops can be quite high but some workers has demonstrated also that legumes can deplete soil nitrogen (Rupela and Saxena 1987;Blumenthal et al. 1982;Tanaka et al. 1983;Yoshida 1982;Ozaki 1969).Most of the data reported on the quantity of N fixed by the legume crops in the WASAT concerned the above ground part of the legume and very little is known on the nitrogen fixed by the roots. Where much of the legume biomass is returned to the soil as green manure, a positive N balance is to be expected. However this may not be true for grain legumes and fodder crops, where the bulk of above legume material is removed from the systems. Nevertheless, many other positive effects of grain legumes such as the improvement of soil biological and physical properties (Hoshikawa 1990) and the ability of some legumes to solubilize occluded P and highly insoluble calcium bounded phosphorus by roots exudates (Gardner et al. 1991, Arihara andOhwaki 1989). Other advantages of crop rotations include soil conservation (Stoop and Staveren 1981) organic matter restoration (Spurgeon and Grimson 1965) and pest and disease control (Curl 1963;Sunnadurai 1973).Cowpea is often the only crop that survives severe drought in the WASAT. Cowpea grain contain about 22% protein, constitutes a major source of protein for resource poor people. It is estimated that cowpea supplies about 40% of the daily requirements to most of Nigeria population (Muleba et al. 1997).In the mixed cropping systems, legumes yields are very low due to low soil fertility, low planting densities and pest and diseases (Ntare 1989;Reddy et al. 1992). The yield of cowpea grain varies between 50 kg ha -1 and 300 kg ha -1 in farmers fields in marked contrast to yield over 2000 kg ha -1 obtainable on research stations and by large scale commercial enterprise in pure cropping. In the mixed farming systems of the WASAT, increasing legume component in the farming systems is important in order to increase the availability of fodder as source of livestock feed while increasing soil fertility.In this paper, after a brief presentation of the cowpea production environment, we will discuss the effect of plant nutrient on cowpea production before reviewing the effect of cowpea cultivation on soil fertility maintenance and presenting the new opportunities for future research on soil fertility and cowpea production.a) Climate Sivakumar (1986) proposed a soil climatic zonation scheme for West Africa that is calculated from rainfall and potential evapotranspiration. In this scheme, a growing period of 60 -100 days was used for defining the Sahelian zone. The geographical extent of the Sudanian zone has an average growing period of 100 -150 days (Figure 1).The rainfall in West Africa shows a significant north-south gradient because of the inter-seasonal movement of the intertropical convergence zone, north and south of the equator. The rainfall is low, variable and undependable. The rainfall gradient is very steep. The further one goes from the Sahara margin, the greater is the rainfall, approximately 1 mm km -1 . The isohyets run nearly parallel (Toupet 1965). Time dependent variations in rainfall are quite common in the region with coefficient of variation of annual rainfall ranges between 15-30%. The data in Figure 2 clearly show the instability of the traditional mean figures for crop production as rainfall in some years can be 50% below or above the long-term average. Nicholson (1981) showed that in 1950, rainfall all over West Africa was above normal, at some location even 250% above normal. However, in 1970 rainfall was below normal throughout the region. As a result of rainfall variability, average yields of sorghum and pearl millet are unstable over years (Figure 3). The data in Figure 4 give the annual rainfall values for a period of 40 years in the Douentza region of northwest Mali. From 1950 to 1990 median rainfall figures dropped from 650 mm/year to 350 mm/year.It is well documented that precipitation determines the potential distribution of terrestrial vegetation and extended drought have initiated or exacerbated desertification. In the past 25 years, the WASAT has experienced the most substantial decline in rainfall (Hulme and Kelly 1997;Hulme 1992;Nicholson and Palao 1993) and the downward trend is persistent since 1951 with more areas experiencing more rainfall variability. As a result of the decrease in rainfall there will be a decrease in the vegetation cover of the land. Because evapotranspiration constitutes the only local input to the hydraulic cycle in the WASAT where there is no significant surface water, a reduction in the vegetation cover logically leads to reduce precipitation (Charney 1975). The reduction of vegetative cover will increase the albedo, which in turn, will lower surface temperature, decrease convection, cloud formation and precipitation. A further decrease in the rainfall will further decrease the vegetative cover (Cunnington and Rowntree 1986;Xue et al. 1990). However, Jackson and Idso 1974 disputed the importance of albedo. The non-climatic anthropogenic forces of desertification includes unsustainable agricultural practices, overgrazing and deforestation.As already indicated, one important consequence in the reduction in rainfall is the reduction of vegetation cover. Consequently, the area of soil left bare and therefore directly exposed to wind and water erosion has considerably increased. The effect of these changes on wind and water erosion are aggravated by the sandy nature of the WASAT soils, which are frequently poorly aggregated, offering little resistance to the erosive forces. Buerkert et al. (1996a) measured absolute soil loss of 190 t ha -1 in one year on bare plots, as opposed to soil deposition of 270 t ha -1 on plot with 2 t ha -1 millet stover mulch (Figure 5). Sterk et al. (1996) reported a total loss of 45.9 t ha -1 of soil during four consecutive storms. Buerkert et al. (1996b) reported that in unprotected plots up to 7 kg of available p and 180 kg ha -1 of organic carbon are lost from the soil profile within one year. Wind erosion will also decrease the exchangeable bases and increase soil acidification (Table 1).Soils Entisols and Alfisols occupy most of the landscape for rainfed cropping in the WASAT. Entisols are mainly composed of quartz sand, with low water and nutrient holding capacity. The Alfisols have a clay accumulation horizon a high base saturation because of lower rainfall and leaching but they have poor structural stability, poor water and nutrient holding capacity and lower organic matter than the Utisols and Oxisols of the humid areas of the rainforest.The data in Table 2 show physical and chemical properties of soils from the WASAT. The soils have low organic carbon and total nitrogen content because of the low biomass production and a high rate of decomposition. One striking feature of these soils is their inherent low fertility which expressed in low level of organic carbon (generally less than 0.3%) total and available phosphorus and nitrogen and effective cation exchange capacity (ECEC). About 98% of the soil nitrogen is stabilized in organic matter. Thus the total nitrogen in the soil and the amount of nitrogen released for plant nutrients uptake will depend on the organic matter level of the soil. Soil total organic carbon is highly correlated with the clay content of soil and as a result of the sandy nature of the soils in the WASAT, total nitrogen remain very low in most of the soils in the region. The importance of soil textural (clay and silt) properties for the organic carbon content was stressed repeatedly as clays are important component in the direct stabilization of organic molecules and micro-organisms (Amato and Ladd 1992;Greenland and Nye 1959;Feller et al., 1992). Thus Feller et al. (1992) reported that independently of climatic variations such as precipitation, temperature and duration of the dry season organic carbon content increased between 600 and 3000 mm annual rainfall soil organic carbon will increase with the clay and silt content of low activity clay soils. This is much evidence for a rapid decline of organic carbon levels with continuous cultivation in the sandy WASAT (Bationo et al. 1995). For the sandy soils, average annual losses in organic carbon expressed by K-value (calculated as the percentage of organic carbon per year), may be as high as 4.7%, whereas for sandy loam soils reported losses much lower, with average of 2% (Pieri 1989).The low ECEC is attributed to low clay content and the kaolinitic mineralogy of the soils. Bationo and Mokwunye (1991) found that the ECEC is more related to the organic matter than the clay content, indicating that a decrease in organic matter will decrease the ECEC and then the nutrient holding capacities of those soils. De Ridder and Van Keulen (1990) reported that a difference of 1 g kg -1 in organic carbon results in a difference of 4.3 mmol kg -1 in ECEC.Both total and available P levels are very low and P deficiency is the most limiting soil fertility factor for cowpea production. Apart from low P stocks, the low-activity nature of these soils results in a relatively low capacity to fix added P (Bationo et al. 1995). Phosphorus sorption maxima of the WASAT soil ranged from 27 to 405 mg P kg -1 with a mean of 109 mg P kg -1 . The data in Figure 6 indicate that low quantities of P are needed to be added in the soil to maintain 0.2 ppm P in the soil solution. For example, the sandy loam soil of Gaya with relatively high level of sesquioxydes 35 mg P/kg soil are needed to be added to the soil to maintain 0.2 ppm P in the soil whereas for the sandy Sahelian soil at Sadore only 10 mg P/kg soil is needed. Compared with the Utisols and Oxisols found in the humid tropical regions these soils can be considered to have relatively low P-fixing capacities, hence small additions of P fertilizers will increase available P in the soil and will give significant crop response.At present, most cultivated land in the region losses more N, P and K that it gain and in that zone, continuous cultivation has lead to nutrient mining and loss of topsoil by wind and/or water erosion (Table 3).Although organic amendments such as crop residue, manure or compost are essential in the sustainability of the cropping systems, they cannot prevent nutrient mining. The addition of organic amendments corresponds in most cases to a recycling process which cannot compensate for nutrient exported through crop products. As a result, the use of external input such as inorganic plant nutrient or local sources of P such as phosphate rock is an essential requirement if soil productivity is to be maintained. Thus increase in water use efficiency (WUE) and alleviation of nutrient mining and increase is paramount.Many research results in the region have shown the importance of the improvement of soil fertility for crop production (Mokwunye and Vlek 1986;Pieri 1989;Van Reuler and Jansen 1984;Vander Heide 1989;Bationo and Mokwunye 1991;Sedogo 1993;Delvaux et al. 1993). In the Sahelian zone, the various research work concluded that soil fertility is more limiting to crop and fodder production than rainfall (Penning de Vries and Djiteye1991; Breman and de Wit 1983). The data in Table 4 clearly indicate that the use of mineral fertilizers will significantly increase water use efficiency. The data in Table 5 clearly indicate a significant effect of N on cowpea and groundnut fodder production in different agro-ecological zones of the WASAT. These significant responses for legumes N to indicate that the predominantly sandy soils of the WASAT may be deficient in molybdenum required for efficient symbiotic fixation (Hafner et al. 1992). On the sandy acid soil at Bengou in the Sudanian zone at Gaya, significant molybdenum response was obtained at different level of soil fertility management for cowpea (Figure 7). Mühlig-Versen (personal communication) on a study on the effect of molybdenum and P effect on groundnut and cowpea found that for cowpea found that for cowpea, application of P doubled the above ground biomass compared to the control. Application of molybdenum to the soil was less effective (+29%). The combination of both increased biomass up to 152% over the control. Groundnut responded only marginally to P or molybdenum, but the combination of both increased the biomass by 53% (Synergetic effect) (Figure 8).Legumes such as cowpea have a high P requirement. P is reported to stimulate root and plant growth, initiate nodule formation as well as influence the efficiency of the rhizobium-legume symbiosis (Robinson et al. 1981). It is also involved in reactions with energy transfer, more specifically ATP in nitrogenase activity (Israel 1987). The data in Figure 9 clearly indicate a strong response to P by cowpea cultivars at Ikeme in the humid zone and Kamboinse in the Sudanian zone of West Africa, but there are large differences between cultivars for their response to P. The local Kamboinse variety is a fodder type and the application of P resulted in higher fodder production but lower grain production. As reported by several scientists such as Dwivedi et al. (1975); Khan and Zende (1977); Stukenholtz et al. (1966); Takkar et al. (1976) andYoungdhal et al. (1977) the application of P resulted in significant decrease of Zinc concentration in the cowpea grain (Figure 10) and this can affect the nutritional quality of cowpea (Buerkert et al. 1998).Despite the importance of P in these soils, the use of commercial P fertilizers in the WASAT is limited due to the high cost of imported fertilizers. Several countries in the region however, are known to have phosphate deposits. Direct application of indigenous phosphate rocks (PR) can be an alternative to the use of more expensive water-soluble P fertilizers. This practice would also promote savings in scarce foreign exchange. The effectiveness of PR depends on its chemical and mineralogical composition, soil factors, and the crops to be grown (Khasawneth and Doll 1978;Lehr and McClellan 1972;Chien and Hammond 1978). The data in Table 6 give the relative agronomic effectiveness of Tahoua PR and Kodjari PR in different agro-ecological zone of the WASAT. The data indicate that Tahoua PR agronomic effectiveness outperformed Kodjari PR. These results are in agreement with the chemical composition of the two rocks where the molar PO 4 /CO 4 ratio is 25 for Kodjari PR and 4.88 for Tahoua PR. As soils in Gaya and Gobery are more acidic and receive more rainfall than the Sadore site, the agronomic effectiveness is higher at those sites. The agronomic effectiveness of the leguminous cowpea is not better than that of the cereal pearl millet crop. This is in contradiction to other reports where legumes have highest strategy to solubilize PR than cereals by rhizosphere acidulation (Aguilar and Van Diest 1981;Kirk and Nye 1986;Hedley et al. 1982) and exudation of organic acids (Ohwaki and Hirata 1992).The data in Figures 11 and 12 give the response of cowpea grain and stover to different sources of P fertilizers. The application of P fertilizers can triple cowpea stover production but the higher stover production resulted in lower grain yield. The relative agronomic effectiveness data in Table 7 indicate that Parc-W PR indigenous to Niger agronomic effectiveness varied from 42% to 54% as compared to the water soluble SSP but the acidulation of PR at 50% (PAPR50) with sulfuric acid can increase the relative agronomic effectiveness to 96% for cowpea stover production. For fodder production TSP relative agronomic effectiveness varied from 77 to 91% indicating that sulfur application is needed for a better growth of cowpea.Over the past years, research at ICRISAT-Niger has focussed on the placement of small quantities of P fertilizers at planting stage in order to develop optimum farmer-affordable P application recommendation. For cowpea stover production phosphorus use efficiency increased from 44 kg/kg P with the addition of Kodjari PR to 99 when Kodjari PR is broadcast with hill placement of 4 kg P/ha as 15-15-15 (Table 8) Long-term experiments are practical means to address the difficult issues associated with quantitative assessment of sustainability in agriculture. In summarizing the results of long-term soil fertility management in Africa, Pieri (1986) concluded that soil fertility in intensive arable farming in the WASAT can only be maintained through efficient cycling of organic materials in combination with mineral fertilizers and with rotation with leguminous N 2 -fixing species. The data in Figure 13 clearly indicate that the application of small quantities of fertilizers and the application of crop residue resulted in an increase of cowpea fodder yield to 5300 kg/ha. In researcher on-farm management trials, it was found that pocket application of small quantities of manure (3 t/ha) plus 4 kg/ha of P at seedling time will increase cowpea yield from …….. in the control plot to …… (Figure 14).Despite the recognized need to apply chemical fertilizers for high yields, the use of mineral fertilizers in West Africa is limited by lack of capital, inefficient distribution systems, poor enabling policies and other socio-economic factors. Cheaper means of improving soil fertility and productivity is therefore necessary. Cereal/legume rotation effects on cereal yields have been reported for the WASAT (Bakayoko et al. 1996;Bakayoko et al. 2000;Bationo et al. 1998;Klaij and Ntare 1995;Nicou 1977;Stoop and Staveren 1981;Bationo and Ntare 2000).Isotopic dilution method with 15 N was used to determine the nitrogen fixed by cowpea using pearl millet as non-fixing crop. The data in Table 9 indicate that nitrogen derived from the atmosphere by cowpea varied from 65 to 88% and the total nitrogen fixed by cowpea depends of the level of soil fertility improvement. The quantity of nitrogen fixed by cowpea varied from 27 kg/ha in the control plot to 87 kg/ha in the treatment where the soils were amended with mineral and agronomic plant nutrients.In order to determine 15 N recovery from different cropping systems, labeled nitrogen fertilizers were applied to microplot where pearl millet was grown continuously (M -M) in rotation with cowpea (C -M), in rotation with groundnut (G -M), intercropped with cowpea (C/M -C/M) and intercropped with groundnut (G/M -G/M). The data in Table 10 indicate that at Sadore in 1990, nitrogen use efficiency increased from 20% in continuous pearl millet cultivation to 28% when pearl millet was rotated with cowpea. For both Bengou and Sadore, nitrogen derived from the soil was better used in rotation systems than with continuous millet cultivation. Bationo and Ntare (2000) carried long-term experiments to investigate the effect of continuous monoculture as compared to crop rotation. At all the three sites in the WASAT, rotation of pearl millet with groundnut and cowpea resulted in higher significant pearl millet yields than in monoculture cropping of pearl millet over the 4-year period (Figures 15 and 16). Legumes also gave significant responses to rotations (Figures 17) and this suggest that factors other than N alone contributed in the yield increases in the cereal-legume rotations. Bagayoko et al. (2000) in studies of cereal legumes effects on cereal growth in the WASAT reported that the rotation effect although significant in most of the cases varied with sites and years. At Sadore as an example, whereas grain yield of pearl millet in 1998 was 1557 kg/ha in the continuous millet production, the millet rotated with cowpea yielded 1905 kg/ha and in Gaya for the same year, sorghum grain yield increased by 50% due to rotation with groundnut in the Sudanian zone (Table 11). The data in Tables 12 and 13 show higher level of mineral N and native arbuscular mychorrhizae in the rotation system as compared to the continuous cultivation of cereals. In sorghum-groundnut system in the Sudanian zone, nematode densities were consistently lower in rotation system compared to continuous sorghum cultivation (Figure 18). Bationo et al. (2000) studied nitrogen dynamics in different cropping systems. In order to determine N availability, the soil were incubated and mineral nitrogen determined at 7, 21, and 35 days(Keeny 1982). Crop rotation significantly affected mineral nitrogen (Figure 19). The fallow millet rotation supplied more nitrogen than the cowpea-millet rotation, but the latter was more productive for millet production. These results suggest that other factors in addition to biological nitrogen fixation may be involved in the positive effect of legume cereal rotation (Crookston et al. 1988). Crop rotation is known to substantially increase soil microbial activity and this may lead to an increase in nutrient availability.In the long-term field trials carried out on the sandy Sahelian soil of the Sahel to study the effect of N and P in different cropping systems, the data show that P application has a very significant effect on yield of cowpea and pearl millet and rotation performed better than continuous cultivation of both crop (Figure 20). The data in Table 14 indicated that the land equivalent ratio varied from 24% to 200% showing that even with the use of external input, intercropping is better than pure cropping. In this long-term cropping system experiment, it was found higher level of organic carbon in the rotation systems as compared to the continuous cropping systems due in part of the fall of cowpea leaves (Figure 21).In another long-term soil management trials, application of phosphorus nitrogen, crop residue, and ridging and rotation of pearl millet with cowpea were evaluated to determine the P use efficiency. The results show that soil productivity of the sandy Sahelian soils can very significantly increased with the adoption of improved crop and soil management technologies. Whereas the absolute control recorded 33 kg ha -1 of grain yield, 1829 kg was obtained when phosphorus, nitrogen and crop residue were applied to plots that were ridged and followed leguminous cowpea. The plots without rotation yielded 1146 kg ha -1 without rotations. Results indicated that for grain yield, P use efficiency will increase from 46 with only P application, to 133 kg/kg P when P is combined with nitrogen and crop residue application and the crop is planted on ridges (Table 15).In the mixed traditional cropping systems cowpea is grown between cereals at very low density as the farmers primary goal is to produce cereal for their family subsistence, and consider the additional cowpea as an additional benefit. This means that farmers need to be assured of sufficient cereal harvest to feed their families before integrating more cowpea in the cropping systems. The yield of cowpea grain in the mixed systems is very low, varying between 50 kg and 300 kg ha -1 in marked contrast to over 2000 kg ha -1 realized at research station and by large scale commercial enterprise in sole cropping. In addition to the low planting densities, pests and disease control, the inherent low fertility of the soil in the WASAT (particularly P) is one of the major constraint to cowpea production in the region and soil fertility replenishment should be an integral part of any program aimed at reverting trend in cowpea production and the conservation of the environment.Phosphorus is the most limiting plant nutrient for cowpea production in the WASAT and there is ample evidence that indicates marked differences between cowpea genotypes for P uptake. Understanding the factors affecting P uptake such as the ability of plants to (i) solubilize soil P through acidification of the rhizosphere and the release of chelating agents and phosphate enzymes (ii) explore a large soil volume and (iii) absorb P from low P solution would help increase cowpea production and yield in the semi-arid tropics.The available and total P values are very low in the region. With these extreme low values of total P, selecting cultivars adapted to low P condition would not be feasible as one cannot mine what is not there. Direct application of indigenous PR can be an economic alternative to the use of more expensive imported water-soluble P fertilizers. The effectiveness of mycorrhizal in utilizing soil P has been well documented (Silberbush and Barber 1983;Lee and Wani 1991;Daft 1991). An important future research opportunity is the selection of cowpea genotypes that can efficiency associate with vesicular-Arbuscular Mycorrhizal (VAM) for better utilization of P applied PR.Cereal/cowpea rotations have led to increased cereal yields at many locations in the WASAT. Factors such as mineral nitrogen, (VAM) for P nutrition improvement and plant parasitic nematodes have been identified as mechanisms accelarating the enhanced yield of cereals in rotation with cowpea. Most of the research quantified the above-ground N fixed by different cowpea cultivars, but very little is known on the below-ground N fixed by cowpea. In the WASAT, most of the above-ground cowpea biomass are used for animal feed and not used as green manure. Future research need to focus more on the on-farm quantification of the below-ground N fixed by cowpea in order to identify the best cultivar for soil N. The identification and alleviation of technical and socio-economic constraints in order to increase cowpea component in the present cropping systems needs attention in future. As cash crop, farmer will increase their credit access to external inputs such as fertilizers. The enhancement of cowpea component in the present cropping system will not only improve the soil conditions for the succeeding cereal crop, but will provide good quality livestock feed, and the manure produced will be of better quality for soil the fertility amendment.Relative losses of a bare compared to a protected (plastic mulch) soil at two depths --------------------------------------[kg ha -1 g -1 ]- ---------------------------------- Figure 21: Effect of phosphorus and cropping system on soil organic carbon, Sadore, Niger 1995 perceived as the most efficient and sustainable means of food production. The evolution process often include paddocking of livestock on cropland in high potential areas; shift to the system of collection, processing, storage and application of animal dung and urine; shift from field grazing of crop residues and pastures to confined livestock feeding; replacement of hand labor with animal traction and mechanization; and intensification through growing of multipurpose legumes and forages. This calls for new research approach that allows replacement or refinement of old paradigms with new principles notably identification of \"best-bet options\" and strategies using \"whole farm\" or holistic approach to working with farmers. Such an approach has resulted in new lessons and insights in management and augmentation of nutrients through crop residue and manure management including livestock-mediated nutrient cycling, crop combination and crop geometry, livestock feeding studies, effect of livestock component on the soil fertility (chemical, physical and biological properties), and also gender, policy and institutional issues.The major challenge facing researchers attempts to contribute towards sustainable intensification of the crop-livestock systems in SSA, is that of soil degradation whereby poor quality and low inputs of crop residues and boma manure leads to soils of low productivity. Inorganic inputs are often too expensive for the low-resource endowed farmers. In such systems, the demand for organic inputs e.g. manure is likely to increase in response to system intensification. The contribution of manure management to enhance soil productivity is unquestionable (Murwira et al., 1995;Pankhurst, 1990). However, there is need for such research to be viewed realistically, especially in the context of the evolving farming systems in the arid and semi-arid lands, notably integrated systems in terms of crops and livestock units which may hinder or compliment each other. There is a growing recognition of the need to develop technologies and policies which ensure optimization enterprises. Implicit in this strategy is the maximization of the contribution of the livestock unit to soil fertility improvement while addressing challenges of increasing intensity of crop-livestock systems.In this paper we will first discuss the effect of manure on soil productivity and ecosystems services. This is followed by highlighting the management practices to increase manure use efficiency before elaborating on the emerging new research opportunities in soil fertility management to enhance the crop-livestock integration.In the mixed farming systems that characterizes the semi-arid zone of Africa, low rural incomes, high cost of fertilizer, inappropriate public policies and infrastructural constraints prevent the widespread use of inorganic fertilizers. Under this situation and as population pressures increases and fallow cycles are shortened, organic sources of plant nutrients such as manure, crop residue and compost remain the principal sources of nutrients for soil fertility maintenance and crop production (William et al., 1985). Estimates of the nitrogen contribution from manure to the total N input budget suggest that up to 80% of N applied to crops is derived from manure in both extensive and intensive grazing systems in East and Southern Africa.Several scientists have reported the effect of manure on crop yield increases in Western and Eastern Africa (Bationo and Mokwunye, 1991;Bationo et al., 1998;Mokwunye, 1980;Pichot et al., 1981;Padwick, 1983;Pieri, 1986;de Ridder and van Keulen, 1990;Abdulahi and Lombin, 1978;Powell, 1986;Murwira et al., 1995;Pankhurst, 1990;Kihanda, 1996;Kihanda and Gichuru, 2000;Lekasi et al., 1998;Probert et al., 1995;Kanyanjua and Obanyi, 1999;Gibberd, 1995;Kihanda and Warren, 1998. Most of the studies in the literature have focussed on the responses of crops to farmyard manure (FYM) applications. One of the earliest reported increases to FYM application in subsaharan Africa was by Hartley (1937) in the Nigerian Savannah. It was observed that application of 2 t ha -1 FYM increased seed cotton yield by 100%, equivalent to fertilizers applied at the rate of 60 kg N and 20 kg ha -1 . In Embu, Kenya, FYM significantly increased maize and potato yields in a long-term trial (Gatheca, 1970). The data in Table 1 summarizes the results of a number of trials on manure conducted in research stations in West Africa. The data shows that manure collected from stables and applied alone produces about 20 to 60 kg N/ha in cereal grain and 70 to 178 N kg/ha in stover per tonne of manure.In Kenya, Kanyanjua and Obanyi (1999) observed that within the Fetilizer Use Recommendation Project (FURP) sites (averaged over several sites and seasons) the response to manure application was in the order cabbages>potatoes>maize>cowpea and nitisols gave a higher response than acrisols (Table 2). Kihanda et al. (1988) while evaluating the effects of inorganic fertilizers, lime, FYM and crop residues on the yield of maize in acidic andosol of Central Kenya found that FYM increased maize biomass by 210%, while lime and P increased yields by 115 and 57%, respectively. They concluded that the large response to FYM application might have been due to a reduction in exchangeable aluminium and manganese allowing the plant to establish better rooting system in addition to providing nutrients, particularly potassium.In the Sahelian zone of West Africa, Bationo and Mokwunye, 1991, found no difference between applying 5 t ha -1 of FYM as compared to the application of 8.7 kg P ha -1 as Single Superphosphate and a further application of FYM at 20 t ha -1 only doubled pearl millet grain a compared to the application of 5 t ha -1 (Table 3). Gatheca, 1970 reported that an annual application of 5 to 6 t ha -1 of manure gave higher yields of maize in Kenya than heavy applications of 20 to 30 t ha -1 applied at intervals of four to five years. In the acidic soils of Central Kenya, Mugambi (1978Mugambi ( , 1979) ) noted that application of FYM at 5 t ha -1 increased the potato tuber yield by more than 50% above the control. A combination of the same rate of FYM and P at 100 kg P ha -1 increased potato yield by more than 100% above the control, an indication that P was also limiting in that soil. The data in Table 4 indicates that the application of 3 t ha -1 of manure plus urine produced grain and total bio-mass that were higher as compared to when only manure was applied and crop response to sheep dung was greater than to cattle dung. Research studies indicate that approximately 80-95% of the N and P consumed by livestock is excreted. Whereas N is voided in both urine and faeces, most P is voided in faeces (ARC, 1980;Termouth, 1989). Dar et al., 2001 clearly indicated that in the P deficient soils of sandy sahelian soils, the addition of P fertilizer will increase the efficiency of FYM and hill placement of both FYM and P fertilizer was better than broadcasting (Fig. 1).The data in Tables 5 and 6 respectively from eastern and Western Africa give the variation in the nutrient concentration of manure samples from different sites, indicating that even on the same soil type and rainfall, the response to manure application will greatly depend on the source of manure. Pieri (1986Pieri ( , 1989) ) and Sedogo (1993) summarized the results of the long-term soil fertility experiments initiated since the 1960's. One important conclusion that emerged from the experiments is that application of mineral fertilizers is an effective technique for increasing crop yields in the Sudanian zone of West Africa. However, in the long-run the use of mineral fertilizers alone will decrease crop yields but sustainable and higher production is obtained when inorganic fertilizers are combined with manure (Fig. 2).At Kabete in Kenya, Nandwa (1997), obtained higher yields of maize in a long-term soil fertility management experiments when mineral fertilizers are combined with crop residue and FYM (Fig. 3).For a modest yield of 2 t/ha of maize the application of 5 t ha -1 of high quality manure can meet the N requirement but this cannot meet the P requirements in areas where P is deficient (Palm, 1995). Organic inputs such as manure are often proposed as alternatives to mineral fertilizers, however, it is important to recognize that in most cases the use of manure is part of an internal flow of nutrients within the farm and does not add nutrient from outside the farm and also quantities available is inadequate to meet nutrient demand over large areas because of the limited quantities, the low nutrient content, and the high labour demands for processing and application. The availability of manure for sustainable crop production has been addressed by several scientists. De Leeuw et al. (1995) reported that with the present livestock systems in West Africa the potential annual transfer of nutrient from manure will be 2.5 kg N and 0.6 kg P per hectare of cropland. Although the manure rates are between 5 to 20 t/ha in most of the on-station experiments, quantities used by farmers are very low and ranged from 1300 to 3800 kg ha -1 (Williams et al., 1995). Hiyami and Ruttan (1985) reported that exclusive use of inorganic fertilizers in Africa will increase food production at best by 2% yr 1 , well below the population growth rate, and not even close to 5 to 6% required to reduce poverty and secure food security. Organic sources of nutrients, however, will be complementary to the use of mineral fertilizers (Quinones et al. 1997). Despite its vital role the quantities of manure are not available on-farm for a number of factors. There are simply insufficient number of animals to provide the manure needed. This problem becomes more pronounced especially in post drought years (Williams et al. 1995). The amount of livestock feed and land resources available are also limited. Depending on rangeland productivity, it will require between 10-40 hectares of dry season grazing land and 3-10 hectares of rangeland of wet season grazing to maintain yields on one hectare of cropland using animal maure (Fernandez et al. 1995).Manure production by zero-grazing cattle in Kenya has been estimated as 1 to 1.5 t animal -1 yr -1 (Strobel, 1987). Two animals will be needed to supply a 2 t ha -1 of crop, if the manure were of high quality, but eight animals are required if the quality is low.Ecosystem services are broadly defined to include nutrient cycles, water movement and storage, soil erodibility, pest control and chemical detoxification. These services are key determinants of agricultural productivity and sustainability. Therefore the application of manure and increase in system carbon can be considered to be a major component of sustaining the crop-livestock production systems. Recognition of this fact has led to investment in research on carbon sequestration in tropical agricultural landscapes.The data in Table 7 in the Sahelian zone of Niger clearly indicates that manure application will not only improve the organic carbon of the soil but by complexing iron and aluminium it will also increase P availability. In the long-term soil fertility management trials, although soil organic carbon decrease in all treatments overtime, the organic carbon value was higher in the treatment where crop residue and manure was applied (Fig. 4).Past and on-going research has been focussed on the assessment of the relationship between land management practices and carbon storage. Our current understanding is that the carbon sequestration potential of different organic inputs is an analogous index to that of fertilizer equivalency. Further studies are directed at the assessment of the trade-offs between the use of soil carbon for agricultural productivity and its value for carbon sequestration potential and environmental conservation. This is a relatively new area of research especially on assessing the effect of quantity and quality of organics on soil organic matter fractions and crop yields.Expected benefits from manure application in the context of ecosystem functions include non-nutritional effect on soil physical properties that in turn influence nutrient acquisition and plant growth. The resource, through interactions with the mineral soil in completing toxic cations helps to reduce the phosphorus (P) sorption capacity of the soil.Manure quality varies widely and clear indices of quality determination are sometimes difficult to apply widely. Past research has been focussed on evaluating different ways of managing manure to improve its quality. Preliminary studies suggest that feeding of concentrates; zero-grazing rather than traditional boma; manure stored under cover instead of in the open, concrete floor rather than soil floor results in higher quality of manure (Lekasi et al., 1998).The quality of manure has been observed to vary with types of animals and feeds, collection and storage methods (Mueller-Saemann and Kotschi, 1994;Mugwira, 1984;Ikombo, 1984;Probert et al., 1995;Kihanda, 1996). A study in Ethiopia showed that the quality of manure declined in the order of chicken > sheep/goat > horse/donkey > cattle manure with respect to % N (1.5, 0.7, 0.5 and 0.4), % P (0.4, 0.4, 0.3 and 0.2) % K (0.8, 0.3, 0.3 and 0.2) and % organic matter (29, 31, 22 and 16), respectively. In a related study conducted in Kenya, Lekasi et al. (1998) observed that the nutrient contents (especially N and P) of manure decreased in the order of chicken, pig, rabbit, goat and cattle, with manure mixed with urine having a higher quality than dung alone. Nevertheless, current characterization studies indicate that manure quality is very variable e.g. % N 0.23-1.76; % P 0.08-1.0; % K 0.2-1.46; % Ca 0.2-1.3 and % Mg 0.1-0.5. High quality manure has been defined as that with % N>1.6 or C:N ratios of <10; while low quality manure has <0.6% and C:N ratios of >17. Recent studies have shown poor correlation between manure quality and lignin, polyphenols and soluble fractions of carbon (Kihanda and Gichuru, 2000). Fig. 5 shows the effect of C:N ratio on N mineralization of manures. Fig. 6 shows that the N fertilizer equivalency increases with high N content.Besides animal type, quality of manure can be enhanced through feed manipulation which is more favourable in intensive grazing systems (eg. stall or zero-grazing units) rather than extensive grazing systems (eg. communal or range etc.). In a study carried out in East Africa, it was reported that manure N concentration increased by more than two fold when the basal diet of barley straw animal feed was supplemented with poultry waste and high quality forage shrubs, Calliandra and Macrotylama (Delve, 1998). In another study, manure from animals that received P supplements of Busumbu (0.70% P) and Minjingu rock phosphate (0.45% P) increased by two to four-fold above the basal diet of napier (0.24% P), bone meal (0.50% P). However, feeding animals with \"unga\" commecial feed resulted in much higher values of P in manure (0.95% P).(b) Composting techniques and materials While the quality of materials used to make compost manures determines its quality, composting techniques are equally important. High quality manures is often obtained from covered shed composting compared to open-shed composts; and similarly from pit composts compared to heap or surface composting. Furthermore crop residue incorporation has been found to minimize nutrient losses through aerobic volatilisation or anaerobic dentrification. For example, in a study in Kenya it was reported that composting low quality manure with different proportions of either tithonia or lantana, the N content of manure was increased by between 10 and 40 per cent depending on the treatment but no changes in P concentration was found (Kihanda and Gichuru, 2000).In a study conducted in Zimbabwe, investigating manure nitrogen changes during storage, Nzuma and Murwira (1998) showed that total N measured in anaerobic manure composts at the end of storage was significantly higher than in aerobic manure composts. This aerobic manure compost incorporated with maize straw was 0.9 and 0.6% N for April and July samples respectively, while the values in the absence of straw incorporation were 1.4 and 1.2% N (due to lack of N immobilization). The results also showed that the pH in anaerobic manure compost system ranged from 6.5 to 6.9 while the anaerobic manure composts were more alkaline with pH range of 8.2 to 8.6 (Fig. 7).The effect of composting on the phosphate rock (PR) dissolution has been study by Bado (1985) and Lompo (1984) in Burkina Faso. The local phosphate rock of Kodjari alone or combined with urea was incorporated in two low quality organic materials for composting during 6 months. The RP and the urea were incorporated in the organic materials at the rates of 4kg of PR (25% P2O5) for 100kg and 12 kg of urea for 1000 kg dry organic matter according to the recommended rates (Lompo, 1984). The organic material was a mixture of 75% of sorghum straws and 25% of cattle manure (using as an inoculums). The effect of composting on the watersoluble phosphorous (WSP) balance before and after composting was evaluated.The results (Table 8) indicated that the composting of the organic materials with PR involved an enhancement of the total WSP balance. The total WSP was positive for all treatments. A positive balance of 67% to 796% of the total WSP was observed after 6 months of composting. The augmentation of the total WSP may be explained by an increase of the soluble phosphorous from organic matter. It may also due to a probable dissolution of the P of the PR by the organic acids during the composting. May be the two process took place during the composting time.(c) Handling and storage techniques Besides heaps and pits, manure may be collected and stored in cattle kraals, bomas, open areas etc. Recent research shows that quality of manure may be affected depending on prevailing conditions. Murwira (1993) reported that under aerobic and high pH conditions in the Kraal, volatilisation of ammonia may occur while the wet soggy anaerobic conditions may lead to dentrification and leaching losses. Such losses are minimized under intensive grazing system such as zero-grazing units with concrete floor and covered roof. In such systems provision of low quality organics as bedding helps to trap the nutrients from the urine. Lekasi et al. (1998) reported that manure removed from grazing units with a soil floor had a much lower N and P and higher ash content than manure removed from grazing units with a concrete floor. Factors responsible for enhanced gaseous N loss in composting include increased total N of the material; high temperatures, low pH and frequent turning (Dewes and Hunsche, 1998). On the other hand high dentrification loss are often associated with increased pH and not increase of insoluble carbon compounds as opposed to reducing sugars under anaerobic conditions. Run-off and nitrate leaching losses can also be substantial.The beneficial effects of combined manure and inorganic nutrients on soil fertility have been repeatedly shown, yet there is need for more research on the establishment of the fertilizer equivalency of the manures and also determining the optimum combination of these two plant nutrients (INM) taking into account the high variability in the quality. Such information is useful in formulating decision support systems and establishing simple guidelines for management and utilization of the resources. Studies investigating the benefits of sole versus combined application of manures and inorganic fertilizers have given variable and sometimes inconsistent results. At Chisunga N in 100% inorganic and 100% organic have yield of maize lower than combining the two plant nutrient. For example, the application of 100 kg N/ha in the inorganic farms have maize yields of about 3.2 t ha -1 but the application of the same quantity with half inorganic form and the other half in inorganic form gave maize yield close to 6 t ha-1. In Manjoro there was no advantage to combine organic and inorganic plant nutrients (Fig. 8). For example, studies in Tanzania indicated that there was no significant difference in maize yields between sole and combined application of 5 t ha -1 of manure and 60 kg N ha -1 of mineral fertilizer (Richard, 1967). But at a different site in the same country, combination of manure at 5 t ha -1 with 40 kg N ha -1 mineral fertilizer gave similar maize yield with either manure at 10 t ha -1 or mineral fertilizer at 80 kg N ha -1 (Kalumuna et al., 1999). Disparities in such responses are partly due to addition of different rates and quality of nutrients through compared treatments; and also due to differences in the limiting nutrients and soil moisture at the test sites. For example, in Madagascar, Rabeson (1992) observed that supplementing manure with inorganic N fertilizer, the rice yield increased by more than 100% but supplementing with P did not improve the crop yield, suggesting that N was the most limiting nutrient in that soil. Another cause of inconsistent results may be due to depressing or antagonistic effects of the nutrient source combinations. For example, a study in Zimbabwe showed that while increasing rates of manure, lime and NPK mineral fertilizers increased growth of pearl millet, however, lime had a depressing effect on the effectiveness of manure while the NPK fertilizers increased the effectiveness of manure (Mugwira, 1985). Also, short-term trials do not give a true picture of the long-term effects of the treatments. Additionally, higher fertilizer equivalencies have been observed in less moist and less fertile soils e.g. sandier and drier soils (Kimani et al., 2001). Using data collected in different sites, Mutuo et al., 2001 found a linear relationship between the percent fertilizer equivalency and the N content. This linear function indicates that increase of 0.1% N in the tissue of organic amendment, there is a 6% increase in the fertilizer equivalency value and the critical level of N content of organic material for net immobilization or mineralization was found to be 2.2%. This is an agreement with the one of the 2.2% suggested by Palm et al. (1995) and Palm et al. (1997) in the decision tree for the selection of organic materials (Fig. 9).(e) Time frequency and method of application Low quality manure is often observed to depress crop yields. This deleterious effect can be overcome through application of manure ahead of planting time to overcome this effect. In some cases, surface application has resulted in better results than incorporation. In many cases, this depends on the quantity of manure applied. Some studies have investigated the potential to overcome this problem through megadose application instead of annual applications. But such studies from Zimbabwe suggest that there are no differences in crop yields between the two application regimes eg. 7 t ha -1 annual application, 14 t ha -1 applied every second year and 28 t ha -1 applied every fourth year (Mugwira and Murwira, 1997).(f) Fortification and pelleting The bulky nature of manures and low quality constraint its transportation and returns to application. To make manure as a biofertilizer easily handleable (less bulky) and applicable, some studies have shown granule pelleting to be a farmer user-friendly packaging system. Other studies have demonstrated that the quality and return to such biofertilizers can be improved through fortification with the addition of inorganic nutrient sources; composting under cover to minimize leaching and loss of nutrients via gases; and the use of high quality biofertilizers on high value crops solely or in combination with inorganic fertilizers.In high external input systems, large quantities of maize stover or wheat straw can be generated (8-10 t ha -1 ), and this is either burnt or partly grazed, resulting in large nutrient offtakes unless the manure is recycled. To overcome this constraint, fortification trials have been conducted. Okalebo et al. (2000) found that combined application of composts of 2 t ha -1 of wheat straw or soybean trash with 80 kg N ha -1 of mineral fertilizer resulted in higher maize yields (grain and stover) than from application of 80 kg N ha -1 of mineral fertilizer alone. Sole application of residue depressed yields. In related studies Muasya et al. (2000) found that wheat straw composted with inorganic fertilizer (80 t ha -1 compost) resulted in slightly higher wheat yields (3.6 t ha -1 ) than with the same rate of normal compost (3.0 t ha -1 ).In both regions, manure is produced abundantly under extensive systems eg. in pastoralists and transhumant systems. As these systems decrease, settled arable agriculture increases. In the latter systems farmers own cattle either under confinement or paddocking systems. Lots of manure is accumulated in cattle boma in the East African region. In West Africa, kraaling eg. keeping of livestock on selected areas over a given period of time, helps increase and accumulate manure through urine and dung voided in the field. Recent studies have shown that two nights kraaling results in between higher yields than in unkraaled fields (Powell et al., 1998).Past reviews of research on the use of organics (with or without mineral fertilizers) for soil fertility management in tropical agroecosystems (CABI, 1994;Padwick, 1993;Nandwa and Bekunda, 1998;Palm et al., 1997;Palm et al., 2001) have shown widespread non-adoption or low adoption of emerging technologies. It has been reported that often the use of organic materials is based on trial and error (Palm et al., 2001). At the research and development level, presently and in future, there is a need for priority setting (Kilambya et al., 1999) and targeting of a potential \"best-bet\" technology for smallholder farmers in the form of an agronomic superiority, economic viability, environmentally friendly and culturally acceptable options.Wider adoption of soil productivity technologies requires that their profitability for smallholder farmers be carefully evaluated. The imperative for future manure research is to adopt a holistic framework for closer interaction between soil productivity subject matter specialists, economists, environmentalists, extensionists and policy makers. There is a need for more horizontal and, above all, vertical networking to create momentum and synergy in soil productivity management research. Lack of multidisciplinary research has been reported to lead to inadequate discounting of soil quality by economists in the context of a \"future generations sustainability quest\" (Young, 1998). Furthermore, other workers have reported a poor relationship between farm product price and nutrient withdrawal (mining) in the context of nutrient replacement cost. Recent work in Kenya showed that 32% of the average net farm income amounted to the replacement of mined nutrients of many farms and farmers, 54% of whom are estimated to live below the poverty line, i.e. on one US dollar per day (de Jager et al., 1998). The proposed new approach should provide synergies between applied or strategic research to adaptive research, and also between farmers' indigenous technical knowledge and their main scientific knowledge, and therefore result in higher rates of technology adoption. Future multidisciplinary research should also investigate yield depression attributed to phytotoxicity associated with manure management (Elliott et al., 1978), plant diseases (Cook et al., 1978) and pests (Musiek and Beasley, 1978).There is a need for a shift from a top-down to bottom-up research approach because the use of the former approach in soil productivity management consultative/collegial research in the past has proved retrogressive, especially for heterogeneous, risk-averse farm households. Future manure and other nutrient input management research should use participatory research approaches eg. farmer's field school, participatory learning action research (Defoer et al., 1998), in the context of the target farming systems, integrating different disciplines and with participation of farmers (Martin and Sherington, 1997;Haverkort et al., 1991).A majority of manure are often characterized as intermediate-low-quality resources and hence are prescribed to be used in a mixture with mineral fertilizer (Palm et al., 2001). Future research is required to identify the \"best-bet\" low-quality manure that can be mixed with high-quality organic resources to satisfy the short-term goal of nutrient availability and the long-term goal of building SOM. Such research should come up with cases for proper discounting of resource conservation estimates (Smaling et al. 1997).The benefits of manures, like other organics, over mineral fertilizers is both the short-term effects and residual or long-term effects. Future research opportunities include the development of guidelines that link quality of manure to their short-term fertilizer equivalency value and longer-term residual effects through SOM turnover and formation.6. Future research opportunities exist on building on past Organic Resource Database (ORD) to develop Decision Support System (DSS) guides and simple tools, based on both scientists and farmer perspectives to guide the choice and utilization of manure depending on their varied quality and quantities. This will require research that correlates scientific indicators (chemical content and nutrient release) with farmers indicators of manure quality (texture, colour, smell, white fungi/sand, homogeneity and longerity of composts).7. Research opportunities exist on the establishment of the relationship between manure quality and a number of variables that influence quality eg. feeds manipulation, composting techniques, manure handling and storage method. These type of research should include determination of strategies that minimize nutrient losses and leaching, erosion, volatilisation and dentrification.8. Future research opportunities include the development of a systematic framework for investigating integrated nutrient management based on fertilizer equivalence values and pertinent ecosystem services and functions. This research should conduct the determination of economic and social trade-offs of improved soil fertility management alternatives to manures eg. legumes, high quality organics, green manures, forage legumes in traditional mixed farming systems. 9. There are future research opportunities on the determination of the biophysical and socioeconomic boundary conditions for the adoption of manure management-based techniques.There is considerable information on manure management in Western and Southern Africa. The results of the comparative analysis from the regions suggest that different lessons can be learned from each stakeholder. As an example, it is clear that scientists in West Africa can benefit by learning more of the technologies developed in West Africa where compost with manure is fortified with phosphate rock and scientists in West Africa can also learn on the work done in East Africa on the assessment of manure fertilizer equivalency, the technologies based on the identification of the best combination ratios of organics and inorganics and the systematics characterization of manure for its nutrient contents and lignins and polyphenols in order to apply the organic matter decision tree.Crop response to manure or in combination with inorganic fertilizers is variable and site specific. The difference in response may be due to several factors eg. soil fertility status quality of manure and environmental factors. This means that modelling and decision support systems will have an important role in future research. Other new research opportunities include topics such as the crop livestock trade-off by developing new strategies that minimize competition between crops and livestock such as conflicting demands of crop residue for feed and soil cpnservation, the legume for soil fertility management per se of feed for livestock the increase of inorganic fertilizer use efficiency, due to better management of manure, the relationships between manure quality and build-up of soil organic matter, the other benefits of manure use and the socio-economic and policy implications. Kanyanjua and Obanyi (1999). (Hemingway, 1961) 1.76 0.24 1.29 0.74 0.34 Kenya (Ikombo, 1994) 1.62 0.50 1.34 0.26 Nd* Kenya (Kihanda, 1996) 1.19 0.24 1.46 0.97 0.26 Zimbabwe (Mugwira, 1984) 0 Primary forest conversion for subsistence agriculture, industrial logging and pasture establishment continues to be the predominant cause of tropical deforestation (Laurance, 1999). These activities have left a large portion of the tropical biome disturbed and in various states of natural regeneration (Brown and Lugo, 1990), stagnation (Fearnside and Guimaraes, 1996;Sarmiento, 1997;Silver et al., 2000), or managed recovery (Fernandes and Matos, 1995;Parrotta et al., 1997). Of the estimated 58.8 million ha of forest cleared in Brazilian Amazônia over the past three decades (INPE, 2002), approximately 24 million ha were converted to pastures (Serrão et al., 1995). Depending on management (replacement of exported or lost nutrients, stocking rates, burning frequency, etc.), region, and soil type, pasture productivity may decline after 7 to 10 years and may be recleared or abandoned to recolonizing secondary vegetation; approximately 50% of the first-cycle pastures have reached this advanced stage of degradation (Serrao et al., 1993). Based on an analysis of 1990 land use data in the Amazon, Fearnside (1996) calculated an equilibrium will be reached where ~47% of all deforested land would be regenerating forest on degraded or abandoned pastures.Although highly altered, these lands are valuable for human use (Brown and Lugo, 1990), and provide important ecosystem services such as watershed protection, sources and havens of biodiversity, erosion prevention, soil fertility recovery by improved fallows (Szott et al., 1991), and atmospheric C sinks (Fearnside andGuimaraes, 1996, Silver et al., 2000). However, the potential of the abandoned land to recover and maintain these roles is dependant on the intensity of previous land use (Uhl et al., 1988;Nepstad et al., 1990;Aide et al., 1995;Alves et al., 1997), soil nutrient limitations (Cochrane and Sánchez, 1982;Smyth and Cravo, 1992;Laurance et al., 1999), and seed inputs and seedling establishment (Nepstad et al., 1996). These impediments to vegetation regrowth may be more extreme in abandoned pastures compared to agricultural land, resulting in lower aboveground productivity (Fearnside and Guimaraes, 1996;Silver et al., 2000), and longer regeneration times.Pasture productivity declines rapidly with decreasing soil P availability, facilitating invasion by secondary forest (SF) species better adapted to infertile soil (Toledo and Navas, 1986); yet, soil fertility and biomass recovery is variable and dependant upon several factors. Degraded pastures are characterized by depleted soil nutrient stocks, low vegetation biomass, low primary forest seed inputs, high seed predation, depleted seed bank of forest species and low stump sprouting (Nepstad et al., 1990), as well as soil surface sealing and compaction (Eden et al., 1991). Consequently, predicting the long-term growth rate of secondary vegetation on degraded pastures and the return of primary forest characteristics becomes a complex task.New attention has focused on fast-growing SF due to their potential to sequester large quantities of C in short time-periods. For example, worldwide tropical forests store approximately 206 Pg C in the soil (Eswaran et al., 1993), and tropical SF of less than 20 years have the potential to accrue soil C at a rate of 1.3 Mg ha -1 yr -1 (Silver et al. 2000). The growth rate of young SF is expected to increase with rising atmospheric CO 2 levels (DeLucia et al., 1999); however, high C allocation to short-lived tissues such as leaves and faster turnover of litter C may limit the potential C sink (Schlesinger and Lichter, 2001). Furthermore, soil nutrient limitations may constrain primary productivity under CO 2 enrichment (Oren et al., 2001).Soil nutrient impediments to productivity under native vegetation are substantial in the Brazilian Amazon. Cochrane and Sánchez (1982) estimated that only 7% of the land area is free from major plant growth limitations; soil P deficiencies (<7 mg/kg) constrain productivity in 90% (436 million ha), and Al toxicity (Al saturation of ≥ 60%) occurs over 73% of the Brazilian Amazon. Low soil Ca (Smyth and Cravo, 1992) restrains productivity, P deficiencies (Gehring et al., 1999) limit SF growth, and the vegetation is unable to effectively capture leaching soil N (Schroth et al., 1999). Mismanagement may compound these deficiencies since pasture use-intensity appears to negatively influence regenerating vegetation biomass (Uhl et al., 1988) and nutrient stocks (Buschbacher et al., 1988). Because SF recovery is variable and dependent on previous land-use and soil fertility, the magnitude and rate of the above-and below-ground C accumulation in these regenerating SF is still relatively unknown.Determining nutrient constraints to regrowth and the status of secondary vegetation is an important step in managing and/or enhancing abandoned site rehabilitation. We examined the dual roles of SF to rehabilitate site productivity and to increase C sinks and investigated potential soil nutrient limitations to these two processes. We examined aboveground and soil C accrual and nutrient stocks in degraded pastures that had been abandoned for a varying number of years. Our objective was to study the influence of regenerating vegetation on C and nutrient budgets following pasture abandonment. We hypothesized that C and N pools would recover with time following post-burn volatilization, while other nutrients would be redistributed from below-to above-ground pools resulting in reduced soil pools.The study areas are located in Amazonas, Brazil, in the central Amazon Basin, north of the city of Manaus along the road BR-174. The study area spans approximately 26 km (2° 34' S, 60° 02' W and 2° 20' S, 60° 04' W). The terrain is undulating with an elevation of 50-150 m. The plateau soil is classified as dystrophic, isohyperthermic, clayey kaolinitic, Hapludox with approximately 80-85% clay (latossolo amarelo according to the Brazilian classification system). Slope soils are composed of Ultisols and valley bottoms by Spodosols. The plateau soils have a low cation exchange capacity and are infertile but are strongly aggregated and well drained (Van Wambeke,1992).The regional climate is tropical humid and the mean temperature is 26.7°C. Mean annual rainfall in Manaus is 2.2 m, with March and April as the wettest months with over 300 mm of precipitation. A mild dry season occurs from August through October, with mean monthly precipitation falling below 100 mm, and in some El Niño years to as little as 50 mm (Lovejoy and Bierregaard, 1990).The native vegetation of this region is closed-canopy, dense, evergreen terra firme forest (Veloso et al., 1991). Species recovery with SF development is significantly different in areas used as pasture compared to areas cut but not managed (Mesquita et al., 2001). Old growth, native vegetation remains the dominant cover in this area. The establishment of new pastures is now rare, and active pastures are a diminishing, short-lived feature of the landscape north of Manaus. However, SF are increasingly found along the primary roads where efforts to raise cattle on large ranches failed some 10 to 20 years ago.A majority of the pastures were mechanically cleared in the early 1980's, commercial timber may or may not have been removed, the slash burned in place or mechanically piled in windrows, and the area planted with exotic African grasses such as Brachiaria brizantha or B. humidicola (Rendle). Standard pasture management for the region includes at least one application of 50 kg P/ha. The animal stocking rate and number of years that the pastures were grazed were variable. Overgrazing and annual burning to increase economic returns in the short-term accelerated pasture degradation through increased nutrient loss and soil compaction. However, even in the absence of overgrazing (1-2 animal/ha) increases in bulk density occur (0.4 g/cm 3 increase from forest values after 12 years as pasture), leading to reduced infiltration, sheetwash, and pasture decline (Eden et al., 1991). Declining pasture productivity is characterized by a reduction in the forage to weed ratio as bare ground develops and herbaceous and woody plants begin to invade. When unpalatable plants begin to dominate, livestock productivity drops, animal mortality increases and the pasture is eventually abandoned. Fire and/or labor intensive hand weeding of seedlings and roots may lengthen pasture life by reducing woody biomass while encouraging grass growth; however, species of Vismia, a fast-growing early successional tree, resprout rapidly after burning and dominate abandoned pastures.Ten SF were selected within three fazendas (cattle ranches) now in various stages of grazing, pasture abandonment or pasture reclamation: Fazenda Rodão (km 46), the Brazilian Agency for Agricultural Research (Embrapa Amazônia Ocidental) Agricultural District of SUFRAMA (DAS) pasture research site (km 53), and Fazenda Dimona (km 72), all along the road BR-174. Within each forest located on plateau Oxisols, we established four plots of 100 m 2 to 400 m 2 , each with three subplots ranging in size from 35 to 225 m 2 depending on forest age. Forests ranged from 0 to 2 yrs to 12 to 14 yrs since pasture abandonment. Secondary forest selection was based on forest age and independence from adjacent plots within the same ranch. We selected a range of forests spanning the age of available SF in the area; however, all SF age classes do not occur at all farms. We conducted farmer interviews to determine site histories and when grazing was abandoned. The date at which the pastures were abandoned is not definitive, as cattle may infrequently graze the area until all palatable forage is replaced by woody successional vegetation. The regenerating forests within the ranches are biologically and physically distinct, each with a unique management history and vegetation cover.Within each subplot, we measured diameter at breast height (DBH at 1.3 m above ground level; Cecropia were measured above prop-roots) for all live tree stems ≥1cm, tagged the stems, and recorded all species. Using two sets of allometric equations, Nelson et al. (1999) for stem >5 cm DBH and Mesquita (in preparation) for those 1 to 5 cm DBH, we calculated dry biomass for each tree and converted the estimates to Mg/ha. The two sets of equations were developed either on the EMBRAPA research site (Nelson et al., 1999) or within the same region (Mesquita, pers. comm). They provide a better estimate of SF biomass than previous equations (Saldarriaga et al., 1988;Uhl et al., 1988;Brown et al., 1989;Overman et al., 1994) developed in the Amazon Basin (Nelson et al., 1999). The Nelson equations provide valid biomass estimates from 1 to 30 cm DBH. However, since these SF have more stems in the smaller diameter range of the Mesquita equations (1 to 5 cm DBH), by using the two sets of equations rather than one, we improve biomass estimates.We used species-specific equations for the dominant tree species Vismia cayennensis (Jacq.) Pers., V. japurensis Reich. (Clusiaceae); Cecropia (Moraceae; mainly C. sciadophylla Mart. and C. purpurascens C.C. Berg); Bellucia (Melastomataceae); Goupia glabra Aubl. (Celastraceae); Laetia procera (Poepp) Eichl. (Flacourtiaceae), and a mixed-species equation for all others. The pioneer Cecropia, uncommon on these sites, occurs less frequently in areas where grazing continues during secondary vegetation establishment (Mesquita et al. 2001).To produce an aboveground forest estimate of nutrient concentrations (and nutrient stocks on a per hectare basis) within each forest, we randomly selected 15 trees ≥1 cm DBH and collected mature, upper canopy sun leaves using a telescoping tree pruner or climbing the boles. From the same trees, we drew two wood core samples (wood and bark) at 1.3 m height on opposite sides of the bole. Foliage and wood samples were pooled into three sample composites of five trees, oven dried at 70ºC, ground and homogenized, and analyzed for C, N, P, K, Ca, and Mg using standard EMBRAPA laboratory operating procedures (Silva, 1999).We developed a foliage:wood ratio (Mesquita, in prep.) for partitioning biomass into wood and foliar components. We then estimated aboveground carbon and nutrient stocks in each forest by multiplying mean nutrient concentrations for foliage and wood samples by the allometric estimates of each biomass component as partitioned by the foliage to wood ratio for individual trees. Our estimates of nutrient pools do not include aboveground biomass <1cm DBH, forest litter, or root biomass.We sampled soil to 45 cm in three depth classes (0-15, 15-30, 30-45 cm) within each of four plots per forest. The four soil samples per depth in each forest (120 soil samples) represent a composite of four to six sub-samples per sample. Soil composites were combined in the field, air dried in solar dryers, charcoal and roots removed, hand milled with a roller, sieved to 2 mm, and analyzed for C, N, P, K, Ca, and Mg. Charcoal is common in local surface soils and is present at times to 45 cm depths in both pasture and forest soils. As charcoal is heterogeneously distributed in the soil, charcoal contamination poses an important impediment to resolution in reporting soil C concentrations. We estimate that carbon concentrations in this study, as with other studies within the Amazon basin, may generally overestimate total soil carbon stocks as a result of charcoal contamination (M.A. Rondón, unpublished data). To reduce the charcoal contribution to soil C estimates, large pieces were removed while the samples were wet and again with a forceps after drying before grinding; however, the small fragment size makes total removal difficult.Extractable soil P and K were analyzed using a double acid extraction (0.05 M hydrochloric acid and 0.0125 M sulfuric acid) and exchangeable Ca and Mg with 1 M potassium chloride. Total soil N was determined by the Kjeldahl technique and soil C (%) by wet digestion (Silva, 1999). Soil nutrient pools (kg/ha) of C, N, P, K, Mg, and Ca were calculated using mean soil bulk density data measured to 45 cm depth from abandoned pastures and SF in the same area (T.R. Feldpausch; S.A. Welch, unpublished data).Nutrient concentrations were multiplied by bulk densities for each depth class to provide soil nutrient stocks on a per hectare basis.Statistical analyses were performed using Minitab 12.1 (Minitab Inc.). Statistical comparisons for C and nutrient concentrations and stocks were conducted separately for the different vegetation tissue types and soil depths using linear and log-linear regression and a p<0.05 significance level. Soil and vegetation concentrations, and soil stocks values were log transformed. Pooling the data for age classes and using regression analysis, we tested for trends in C and nutrients within aboveground and soil pools, as partitioned by depth, foliage or wood, versus time (years after pasture abandonment).A total of 1901 stems were measured in 2320 m 2 , of which 138 standing dead and 177 lianas were excluded from biomass calculations due to allometric equation limitations in computing such components. Of those stems considered in biomass estimations, 68% were less than 5 cm DBH, while no stems were greater than 30 cm DBH. The two recently abandoned pastures of 0-2 years had no stems ≥1cm DBH, the minimum diameter used for the allometric equations.Wood N and P concentrations declined with SF age (r 2 = 0.85, 0.75; p<0.001), with an average reduction of 50 and 60% in wood N and P from the youngest to the oldest forests. Foliage N and P concentrations tended to decline with forest age, although non-significantly. Compared to wood, foliage contained an average of 5.7 times more N and 3.7 times more P (Table 1).Foliar and wood Ca concentrations did not show a trend with age, but the concentrations were high relative to other nutrients. Calcium concentrations in wood were comparable and at times higher than wood N values. n the foliage, Ca concentrations represented an average of 43% of N values. Foliage contained an average of 2.5 times more Ca than wood. Potassium and Mg foliar and wood concentrations showed no trends with forest age.Although woody biomass accumulated more quickly than foliage, nutrient stocks for all nutrients accumulated more quickly in foliage (Figure 1). Foliar N stocks (42.6 kg ha -1 yr -1 ; r 2 =0.94; p<0.001) increased much more rapidly than woody stocks (15.5 kg ha -1 yr -1 ; r 2 =0.91; p<0.01) with time after abandonment. Phosphorus stocks in foliage accrued twice as fast as wood P stocks. However, foliar Ca stocks (22.3 kg ha -1 yr -1 ; r 2 =0.92; p<0.001) accrued at a similar rate to wood stocks (20.6 kg ha -1 yr -1 ; r 2 =0.90; p<0.001) (Table 2).Within each forest, soil carbon and nutrient concentrations generally decreased with depth (Table 3). Total soil N concentrations generally decreased with depth; however, deeper soil profile (30-45 cm depth) N concentrations increased with time after pasture abandonment (r 2 = 0.57; p<0.001), while the shallower depths showed a weaker soil N trend with time. Soil extractable P concentrations tended to decrease at all soil depths over time, with significant reductions in surface layers (0-15 cm depth) with increasing time since abandonment (r 2 = 0.46; p<0.05). Near surface Ca levels (0-15 cm depth) ranged from 0.13 to 0.33 c.mol (+) /kg. Calcium concentrations were low below 15 cm depth, with overall means of 0.07 c.mol (+) /kg at 15-30 cm and 0.07 c.mol (+) /kg at 30-45 cm depths. ------------------------------------------kg ha -1 yr -1 ------------------------------------------ Soil extractable nutrient stocks were generally lower in deeper soil pools. Within the oldest forests, soil C, N, and Mg nutrient stocks were greater than aboveground nutrient stocks while the other nutrients resided predominantly within forest vegetation (Figure 1).Soil N stocks, relative to aboveground stocks, were high, and increased with forest age at a rate of 117.8 kg ha -1 yr -1 (r 2 = 0.44; p<0.05). Pastures abandoned for twelve or more years stored 1.5 Mg/ha more total N to 45 cm depth than areas abandoned for two or fewer years (5.4 and 3.9 Mg N/ha). In all forests, surface nitrogen stocks (0-15 cm) represented approximately 40 to 45% of the total soil nitrogen to 45 cm depth (total 45 cm range: 3.3 to 5.5 Mg N/ha) (Figure 1).Extractable soil P stocks to 45 cm tended to decline with increasing forest age (-0.66 kg ha -1 yr -1 ), a trend most pronounced within the upper 0-15 cm. This surface layer represented 46 to 70% of total soil P stocks to 45 cm depth, with the younger areas, on average, storing 4.2 kg/ha more P in the first 15 cm than the oldest areas. Considering the entire measured soil profile (0-45 cm depth) higher extractable soil P stocks were observed in stands of 0 to 6 years (11.5 ± 4.6 kg/ha) compared to stands of 6-14 years (5.1 ± 2.1 kg/ha). Soil P in the 0-15 cm class was more variable than in deeper layers. Potassium, Ca, and Mg stocks remained constant with time after abandonment (Table 2).There was a significant net gain in combined vegetation and soil nutrient stocks for all nutrients (Table 2). The total system P accumulation rate was slow and reflective of the counteracting decrease in soil P stocks with increasing forest age. Total system N stocks increased most rapidly followed by Ca. While total (biomass plus soil) nutrient stocks for all nutrients increased over time, in soils, only N increased significantly.Standing biomass. Foliar dry biomass in the ten forests grouped according to age after pasture abandonment (0-2, 2-4, 4-6, 6-8, 12-14 years) was 0.0, 0.02, 3.47, 13.10, 32.15 Mg/ha (Figure 1). Average biomass accrual for all SF through the first 12-14 years after pasture abandonment was 11.0 Mg ha -1 yr -1 (r 2 =0.95, p<0.001), or 5.6 Mg C ha -1 yr -1 (r 2 =0.94, p<0.001). As expected, carbon stocks in wood (4.2 Mg C ha -1 yr -1 ) accrued more quickly than foliage C (1.4 Mg C ha -1 yr -1 ) as forests matured (Table 4).Table 4: Relationship between years after pasture abandonment (X) and the accumulation of aboveground biomass and carbon, and soil carbon (Mg ha -1 yr -1 ) in ten secondary forests regenerating from degraded pastures in central Amazônia, Brazil. The greatest total biomass (128.1 Mg/ha) was measured in a SF with 12-14 years since abandonment; the areas abandoned 6-8 years had an average biomass of 54.4 Mg/ha, while the areas abandoned 4-6 years an average of 16.4 Mg/ha (Figure 1). Woody biomass in the ten SF by years after pasture abandonment was 0.0, 0. 05, 12.92, 41.25, 92.24 Mg/ha in the 0-2, 2-4, 4-6, 6-8, and 12-14 yearold forests.Soil Carbon. Soil carbon storage (excluding roots) tended to increase with forest age, with the oldest forests storing an average of 25 Mg/ha (65%) more total soil C to 45 cm depth than the youngest forests. Surface layers (0-15 cm) stored significantly more C (28.4 ± 2.4 Mg/ha) than deeper layers (18.3 ± 1.5 Mg/ha), from 24 to 50% of the total soil carbon to 45 cm depth in all forests (p<0.001). However, the oldest forests, 12-14 yr-old, stored as much carbon in the 30-45 cm layer as in the 0-15 cm surface layer. Additionally, the deeper soil profile (30-45 cm) was the only depth showing significantly increasing C stocks with time after abandonment (r 2 =0.21; p<0.001). Considering all forests, total soil C to 45 cm depth increased non-significantly at a rate of 1.49 Mg ha -1 yr -1 during the first 12-14 yrs of succession (Table 4).Total C accrual. In vegetation and soil (excluding roots), the ten SF accrued a total of 7.04 Mg C ha -1 yr -1 during the first 12-14 years after abandonment (r 2 =0.85; p<0.001) (Table 4).The vegetation withdraws large quantities of exchangeable Ca from low exchangeable soil reserves. After N, vegetation Ca stocks were accumulating most quickly with forest age. Wood and foliage N:Ca ratios were low, ranging from 0.7 to 1.4 for wood and 1.9 to 3.0 for foliage. In contrast, primary forest vegetation reported N:Ca ratios were 3.1 for trunks, branches and coarse roots, and 4.4 for leaves (Fernandes et al., 1997).The high rate of Ca immobilization in vegetation but lack of reduced soil exchangeable Ca over time in our study indicates, (1) soils adequately replenish immobilized Ca from unavailable forms (Table 2); and/or (2) the vegetation is withdrawing Ca from deeper than 45 cm depth. A similar trend of a high percentage of total system Ca content in vegetation and high Ca uptake from low soil reserves of exchangeable Ca has been reported for temperate forests (Johnson and Henderson). The highly weathered Oxisols of our study provide negligible Ca from parent materials; however, atmospheric deposition may replenish depleted soil reserves by adding 0.8-12 kg ha -1 yr -1 (Vitousek and Sanford Jr., 1986;Schroth et al., 2001). For young tropical fallow vegetation, low root length density and low nutrient demand make Ca and nitrate ions susceptible to downward movement (Szott et al., 1999), which may be retrieved with increased rooting depth in later successional stages. Trees have been reported to increase soil nutrient availability over time (Sanchez et al., 1985) and net increases in total system stocks of N and Ca have been observed in older fallows, probably as a result of atmospheric deposition, N 2 fixation and uptake from subsoil (Szott et al., 1991). However, pasture soils were found to have higher exchangeable soil Ca concentrations than plantations, secondary and primary forests (McGrath et al., 2001), indicating that after the initial increase of soil Ca from cutting and burning, colonizing trees act as sinks, reducing soil Ca. Although soil Ca stocks are currently maintained in these SF, the high rate of Ca relocation from soil to vegetation, large vegetation Ca stocks, and high concentrations relative to N indicate extreme Ca demands for biomass production, which may create a soil Ca deficit and limit future vegetation growth.The rapid total soil N stock and N concentration increase below 30 cm depth with forest age can be only partially explained by external inputs (Figure 1, Table 3). Nitrogen fixing plants may contribute 10-150 kg ha -1 yr -1 to soils (Fernandes et al., 1997;Szott et al., 1999) and atmospheric deposition may add 5.5-11.5 kg ha -1 yr -1 (Jordan et al., 1982;Vitousek and Sanford Jr., 1986;Schroth et al., 2001), explaining a fraction of the increasing total soil N. The remaining contribution to the high soil N accumulation rates could be subsoil mining of leached nitrate. Increasing extractable soil N with depth below topsoil have been measured in young SF (J. Lehmann, pers comm.); high deep soil N concentrations may be attributable to leaching from surface layers after slash-and-burning and cropping, followed by a reduced nutrient capture potential of shallow rooted colonizing secondary vegetation. Primary forest also loses nitrate to the subsoil (Schroth et al., 1999). These large N pools were deep (1-2 m) and considered at the lower limit of uptake by young SF. Leaching of surface N can be rapid in Oxisols because of the high macroporosity and hydraulic conductivity, but leaching below 0.6 m is delayed, apparently because of NO 3 adsorption to the net positively charged subsoil (Melgar et al., 1992). Deep nutrient pools may provide a source of N as forests mature and root systems develop. Leaching of surface N (0-15 cm) to deeper layers could also explain the increase in N concentrations (r 2 = 0.75; p<0.001) we observed below 30 cm depth with forest maturation. Unless deep N mining occurs with root development, N losses to subsoil due to leaching may negatively affect surface soil fertility.Compared to primary forest nutrient storage in soil (of the total aboveground and soil stocks), the SF stored comparable amounts of N, less P, but more Ca. Soil storage of exchangeable Ca and total N in the oldest SF accounted for an average of 26 and 89% of total nutrient storage, respectively, but just under 14% of extractable P (Figure 1). This contrasts with compartmentalization within primary vegetation, where soil storage of exchangeable Ca and total N may account for <1 and 73% of total nutrient storage, and extractable P in soil accounts for 69% of the total storage (Sanchez, 1987).Increases in soil nutrient concentrations are followed by greater vegetation tissue concentrations in successional vegetation. Secondary forest vegetation growing on nutrient poor soil produced wood with three times less P and leaves with 50% less P than vegetation where soil P limitations were removed through P fertilizer additions (Gehring et al., 1999). The reduction in foliar P concentrations with increasing forest age observed in our study (Table 1) may indicate that this nutrient is becoming limiting as soil P levels decline (Figure 1).Total aboveground and soil nutrient stocks increased as forests matured; yet, for P, uptake and soil P supply indicates a potential growth limitation. Concomitant decrease in extractable soil P and increase in biomass over time may be attributable to relocation from below-to above-ground pools. Plants appear to be taking up more soil P than is available (Table 2). This suggests a rapid transfer of soil P from plant unavailable to available forms or deep soil mining as the available pool is depleted with plant growth. However, subsoil P retrieval probably contributes <1 kg ha -1 yr -1 (Szott et al., 1999). The net reduction in soil P stocks from the soil (0.66 kg P ha -1 yr -1 ) with increasing forest age, indicates inadequate replacement of available soil P with plant P uptake, a trend also observed elsewhere (Johnson et al., 2001). Should this trend continue, P may become limiting to growth unless other factors (1) reduce P uptake by plants, (2) increase P uptake from subsoil, (3) increase the rate at which unavailable forms of soil P shift to plant available P forms to replenish immobilized plant available soil P.Pools of plant available (extractable) nutrients are significantly lower than the total in soils (Brown and Lugo, 1990), and the plant availability of the soil P depends on the extent of fixation or immobilization. Phosphorus fixation for Oxisols is lower in the central Amazon Basin than Oxisols in other regions of the Amazon; however, levels of plant available P in the soils are similar (Lehmann et al., 2001a). Total soil P in primary forest can be lower than under the SF replacing the vegetation (Lehmann et al., 2001b), indicating storage in biomass can significantly reduce soil extractable P stocks. Pastures grasses such as Brachiaria spp. may increase P availability by exuding acid phosphatase into the rhizosphere and hydrolyzing plant unavailable forms of organic phosphates (Dias-Filho et al., 2001), a benefit lost as secondary vegetation replaces the pasture grasses. Root associations with both VAmycorrhizae and ectomycorrhizae may help the colonizing vegetation access P even at low soil P concentrations; and, in the case of ectomycorrhizae, to access P from poorly accessible pools (Boot et al., 1994). Since these colonizing species have the ability to take up P in excess of immediate growth requirements (Boot et al., 1994), P uptake by maturing trees may decline before plant levels become limiting to biomass accumulation. Fallow vegetation increased mineralizable N and available P compared to continuous cropping, probably as a result of deeper rooting (Tian et al., 2001).Further research is needed to develop and evaluate management strategies that promote soil P acquisition, such as increasing rooting depth of regenerating vegetation.Aboveground. Rapid biomass accrual in the SF, 11.0 Mg ha -1 yr -1 , was similar to other Amazonian findings (Uhl et al., 1988;Brown and Lugo, 1990;Alves et al., 1997), lower (Hartemink, 2001), and higher than a 20 year mean annual rate (6.17 Mg ha -1 yr -1 ) in a review of tropical SF succession (Silver et al., 2000). The high C accumulation storage in the 12-14 yr-old areas represents 25-50% of equivalent primary forest biomass (230 -500 Mg/ha) in Amazonia (Alves et al., 1997;Fujisaka et al., 1998;Laurance et al., 1999).Aboveground carbon accrual slows with age as colonizing trees mature, die, and are replaced by slower growing species. Secondary forests in the Bragantina region of the Amazon basin were accruing biomass more rapidly in 10 yr-old (5.5 Mg ha -1 yr -1 ) than in the 20-40 yr-old SF (3.3 Mg ha -1 yr -1 ) (Johnson et al., 2001). And a review of 44 secondary tropical forests showed wet forests accumulating biomass significantly faster during the first 20 of 80 years of regrowth (Silver et al., 2000). Their rates through the first 10 and 20 years are still less than the rate we report through the first 12-14 years (Table 4).Belowground. Our study indicates a trend of increasing soil C storage through the first 12-14 years (Figure 1); however, the soils are storing comparable to less C than other SF of similar or greater age (Silver et al., 2000); (Johnson et al., 2001). Although our forests only showed a weak C storage gain, other studies indicated that soil C storage (excluding roots) increases significantly with SF age, and can approach mature forests levels after 80 years regrowth (Silver et al., 2000).Contrary to aboveground biomass accumulation rates, which proceed faster in SF following agriculture (Fearnside and Guimaraes, 1996), soils accumulate C almost twice as fast when regeneration follows pasture rather than agriculture, although this effect is only distinguishable after 20 years of recovery (Silver et al., 2000). Delays in aboveground C accrual with forest growth in early years following pasture abandonment may be offset, to a certain degree, by enhanced soil C accumulation.Compared with soil C storage in pasture (49.5 Mg/ha; 0-30 cm depth) (Moraes et al., 1996), tropical plantation (90 Mg C/ha) and SF (61 Mg C/ha) (0-25 cm depth) (Silver et al., 2000), the SF soils in this study were storing 47.9 Mg C/ha to 30 cm and 66.1 Mg C/ha to 45 cm depth. Since aboveground C accrual appears higher than other sites, and soil C lower than other SF sites, high litter turnover and soil respiration rates at our sites may be reducing soil C residence time in this high rainfall area.These factors pose important management implications to carbon sequestration. By choosing to maintain areas as pasture, directing SF colonization and succession after abandonment, or establishing plantations or agroforestry systems, land managers can influence the distribution of aboveground and soil C storage and the rate at which carbon accumulates within those pools. Maintaining the land cover as forest for longer time-periods rather than as degraded pasture is a more favorable practice to increase C storage.The ≥1 cm DBH limitation imposed by the allometric equations may significantly underestimate biomass and nutrient stocks in the absence of root and biomass measurements of young SF vegetation <1cm DBH. Grasses tend to allocate a significant portion of total plant biomass within root structures (Nepstad et al., 1994) and necromass, shrubs and herbaceous vegetation dominating early pasture succession and SF understories contribute considerable quantities to C and nutrient stocks, especially P (McKerrow, 1992). Wood core measurements may overestimate nutrient concentrations in young stands since a greater portion of the sample core is nutrient rich bark. An underestimate of biomass as a result of DBH allometric limitations is inversely related to forest age. As stand dominance shifts from small-to large-stem diameter plants with understory shading and self-thinning, a greater percentage of the total stems are measured and contribute to biomass and nutrient calculations. Also, although we located our abandoned pasture study sites on plateaus, the pastures span the rolling topography. Nutrient limitations may be more severe on hillsides where erosion is more pronounced and forest recovery slower than the rates we predict.After two and a half decades of neotropical studies of SF regeneration, we still lack the ability to make strong predictions about nutrient storage and successional shifts in forest development, and regeneration times for abandoned pastureland to attain primary forest equivalent biomass. This is largely due to an historic research focus on forest succession following agriculture rather than pasture. Since biomass recovery is significantly slower following pasture than agriculture (Fearnside and Guimaraes, 1996;Steininger, 2000), it is important to increase our understanding of pasture succession and determine potential nutrient limitations.The paucity of forest recovery studies on abandoned pastureland and lack of detailed soil C and nutrient data make predicting forest regeneration on highly altered lands difficult. In a review of SF biomass accumulation (Silver et al., 2000), only 13% of the SF (18 of 134) were previously pastures. Additional data from SF regenerating from pasture are needed to determine long-term C accumulation rates, potential nutrient limitations to regeneration, and the time needed to attain both structural and functional properties of mature forests. This is especially relevant since primary forest biomass is positively associated with soil nutrient levels, suggesting that soil nutrient loss through pasture installation may result in lower mature regenerated forest biomass than the original forest (Laurance et al., 1999). Although studies such as ours help to fill this void, there is a need for long-term rather than chronosequential studies of forest recovery following pasture abandonment.In this study, we show that during early successional years, biomass accumulation in light to moderately used pastures is rapid after abandonment and that soil C storage is higher in older forests. However, a slower soil C accrual rate than regenerating SF in other regions, may negatively offset total long-term C gains. The higher proportion of soil C storage compared to aboveground pools will be an important consideration of future 'carbon credit' management, as this pool is more recalcitrant to perturbations. Aboveground C re-accumulation from post-burn values is high, yet represents a finite pool which is rapidly attained in a relatively short time-period. Managing forest regeneration to maximize soil C storage, rather than aboveground pools, may prove to be more useful or meaningful when attempting to increase SF C sequestration.Furthermore, the colonizing vegetation can extract large nutrient quantities from the soil, even when in low supply. There was not only a shift of nutrients from soil to aboveground pools, but total system nutrient stocks were increasing over time. Most of the C, N, and Mg were stored within soils, while P, K, and Ca resided within vegetation. This has important consequences to total forest nutrient stocks, in the event of removal of aboveground vegetation. In the absence of nutrient additions, removal of the vegetation a second time (pasture re-clearing or logging) could compromise the SF potential to regenerate as a result of nutrient limitations. Even after P fertilization when the areas were pasture, soil P stocks remained low. The vegetation was withdrawing more soil P than can be replenished, creating a soil P deficit which may limit system productivity. Low exchangeable soil Ca stocks seemed to be adequately replaced, apparently from atmospheric inputs and depths below 45 cm, as growing vegetation took up large nutrient quantities. Nevertheless, as vegetation Ca demands were high and soil stocks low, lack of Ca may limit future productivity.These results demonstrate the regenerative capacity of tropical SFs to sequester C and to rebuild the nutrient capital following pasture abandonment. Aboveground carbon accrual is rapid but belowground gains represent the largest potential area for continued accumulation and management. Relocation of some nutrients from deeper soil layers may represent a substantial source of nutrients for plant growth and may be vital to sustaining long-term productivity and biomass accumulation. We recommend additional studies to explore P and Ca nutrient limitations to forest productivity and longterm measurements of soil nutrient fluxes and forest growth. Understanding nutrient limitations to resource capture will provide new options to manage forest regeneration and increase C accumulation on these globally important nutrient-limited soils.Upland soils in the humid tropics such as in the central Amazon are highly weathered and therefore possess low plant available nutrient contents (Cravo and Smyth, 1997). This is a result of both high rainfall and low nutrient retention capacity. Applied nutrients are rapidly leached below the root zone of annual crops (Melgar et al., 1992;Cahn et al., 1993). Two basic approaches can be used to reduce nutrient leaching, first to apply slow-releasing nutrient forms such as organic fertilizers or secondly to increase adsorption sites and thereby retain applied inorganic nutrients.Slash-and-burn is one of the main land use system in the Amazon. Secondary or primary forest is cut and burned to clear the field but also to release plant-available nutrients from slashed plant biomass. The ash from the burned biomass increases soil pH and supplies nutrients to crops which show elevated nutritient levels and yields (Sanchez et al., 1983). This effect of the ash accumulation is, however, rather short-lived. Already after a few cropping seasons the soil nutrient availability decreases and field crops have to be fertilized for optimum production (Sanchez et al., 1983) or the fields have to abandoned and new forests have to be slashed and burned. Although adequate applications of mineral fertilizers were shown to sustain yields in the Amazon (Smyth and Cassel, 1995), our efforts are intended to improve the use of biomass and nutrients contained in the plant biomass as well as that of applied fertilizer nutrients, since fertilization is expensive and crop production often has to rely on soil nutrients alone.It is well known that about 50% of the carbon in the above ground biomass of forests can be lost upon burning (Kauffman et al., 1995). Sixty and 43% of the biomass N and S and 18, 7 and 7% of the P, Ca, and K were lost from the site (Kauffman et al., 1995). A large portion may be deposited or absorbed in surrounding ecosystems but does not contribute to the fertility of the cropped soil. The first approach should therefore aim at improving the efficiency of land clearing to preserve C and nutrients. Slash-andmulch was successfully tested in Eastern Amazonia when fertilizer was applied (Kato et al., 1999) and has a long history in the per-humid tropics (Thurston, 1997). We are seeking an alternative technique that can be applied to the existing slash-and-burn system with minimal changes and that has the potential of being used in tree cultures as well.Instead of burning the above ground biomass to clear the agricultural field, the biomass may be charred to produce charcoal and added to soil. Testing the application of charred organic matter was stimulated by the fact that anthropogenic dark earths in Central Amazonia (so-called \"Terra Preta do Indio\") with high soil organic matter contents contain large amounts of pyrogenic carbon (Glaser et al., 2001). These soils also show high cation exchange capacity, nutrient availability and organic matter (Sombroek, 1966;Kern and Kämpf, 1989). The origin of the dark earths is not entirely clear, and several conflicting theories were discussed in the past. Currently the most convincing theory states that these soils were not only used by the local population but a product of indigenous soil management as proposed by Gourou (1949).Soil fertility increases have been observed on remnants of charcoal hearths in the Appalachian Mountains (Young et al., 1996). Tryon (1948) showed higher nutrient availability in clayey to sandy soils from the Western United States after additions of charcoal produced from conifer and hardwood. Coal from geological deposits were successfully tested for the improvement of soil physical properties (Piccolo et al., 1996). No information, however, is available about the effects of charcoal applications on nutrient availability of highly weathered soils in the humid tropics such as the central Amazon. It is also unclear whether the cation exchange capacity can be improved thereby leading to higher nutrient retention and to lower nutrient losses by leaching.A slash-and-char technique does not advocate the destruction of existing primary forests. It should be a carbon-and nutrient-conserving alternative to existing slash-and-burn techniques. In this way, carbon will rather be retained in the system compared to slash-and-burn, since only the biomass from the same cropping area will be used for producing the charcoal.Pot experiment. A greenhouse study was carried out at the Embrapa Amazonia Ocidental near Manaus, Brazil. The mean temperature in the greenhouse was between 28-32ºC. We used two different soils for our experiments: (1) a Xanthic Ferralsol taken from a secondary forest (approximately 15 years old) with high clay contents (65%), medium organic C (39 g kg -1 ) and N contents (31.7 g kg -1 ); (2) a Fimic Anthrosol obtained from a farmers field under fallow with low clay (5%) and high sand contents (85%), high organic C (84.7 g kg -1 ), available P (318 mg kg -1 ) and Ca contents (656 mg kg -1 ), but low to medium total N (49.6 g kg -1 ), available K (4.0 mg kg -1 ) and Mg contents (57 mg kg -1 ). Both soils have not been fertilized prior to the experiment.Free-draining lysimeters were constructed with a diameter of 0.2m and a height of 0.1 m which were filled with either 3 kg of the Ferralsol or the Anthrosol. The effect of soil type, mineral fertilizer and charcoal on growth, nutrient uptake and leaching was tested using rice (Oryza sativa L.) as a test plant.Charcoal was applied at 20% weight which was produced by local farmers originating from secondary forests. The charcoal was ground by hand to a grain size of about 1 mm. Fertilizer was applied at 30, 21.8, and 49.8 kg ha -1 for N, P, and K using ammonium sulfate, TSP, and KCl, respectively. Lime was applied at 2.1 Mg ha -1 [all recommendations for rice from Araujo et al. (1984) Circular Técnica 18, Embrapa, unpublished].After the soil was filled into the lysimeters, water was gently poured onto the soil at a daily rate of 6.85 mm (2500 mm y -1 ). After four days the electrolyte content in the leachate had stabilized and fertilizer was added and rice was planted (five stands per pot with three plants per stand). Water was applied and drained daily, but only selected samples were analyzed. Nutrient contents were determined daily for the first week, twice a week for three weeks and after 5 and 10 days. The sampling was stopped when the rice was cut at 37 days after planting. The amount of leachate was determined by weight and a subsample was retained for further analyses and frozen. Cumulative leaching for the entire experimental period was calculated from the measured leachates and amounts were interpolated linearly. Plant samples were dried at 70ºC for 48 hours and weighed.In a second pot experiment, seedlings of Inga edulis were planted in pots with 26 cm diameter and 10 dm 3 soil (Xanthic Ferralsol) in four replicates. Charcoal was added at 0, 1, 5, 10, and 20% weight corresponding to 0, 13.3, 66.7, 133.4, and 266.7 Mg C ha -1 (C concentration of charcoal 70.8%). Fertilizer was applied at 100 kg N ha -1 , 50 kg P ha -1 , and 60 kg K ha -1 as urea, triple super phosphate and KCl, respectively. Additionally, 2 Mg ha -1 lime were added. Stem diameter was determined at 5-cm above soil level, and tree height was measured including the length of the uppermost leaf at 80 day after planting.Adsorption experiment. In a laboratory experiment, we studied the adsorption of different nutrients by charcoal. The charcoal was made from the wood of black locust (Robinia pseudoacacia). Cubes of dried wood with 10 g were isothermically combusted in closed metal containers at 350ºC for 40 minutes (65 replicates). Wood and charcoal were weighed with an accuracy of 0.1%. The charcoal was ground coarsely with mortar and pestle to pass a 2 mm sieve. One gram of charcoal was added to 10 mL of solution containing 0, 20, 50, 100, 200 mg L -1 using 20-mL PE bottles. In a preliminary experiment, the adsorption dynamics were determined for 10, 30 minutes, 1 and 6 hours, 1 and 3 days and 1 week using a horizontal shaker. Adsorption changed until one day, but did not differ thereafter. Therefore, adsorption experiments were done with a shaking time of one day. The effect of coating with dissolved organic matter (DOC) was tested with a manure extract. Ten grams cow manure were shaken with 20 mL deionized water and filtered. The filtrate was diluted 50 times and 10 mL of the solution was shaken with 1 g of charcoal for 24 hours. Afterwards the same adsorption experiment with different concentrations only of NH 4 + was performed as described above with and without additions of 10% azide to inhibit microbial activity.Chemical analyses. The aboveground biomass of rice was ground with a ball mill and analyzed for nutrients and organic carbon. C and N analyses were performed with an automatic CN analyzer (Elementar, Hanau, Germany). The K, Ca, Mg, Fe, Zn, Cu contents in the plant biomass were determined after wet digestion with sulfuric acid using atomic absorption spectrometry (AA-400, Varian Associates, Inc., Palo Alto, CA). The P contents were measured photometrically in the same extract with the molybdenum blue method.The K, Ca, and Mg contents in the leachate and adsorption solution were measured using atomic absorption spectrometry, nitrate (NO 3 -) and ammonium (NH 4 + ) concentrations were determined photometrically with a continuous flow analyzer (RFA-300, Alpkem Corp., Clackamas, OR and Scan Plus analyzer, Skalar Analytical B.V., Breda, The Netherlands) after reduction with Cd and reaction with salicylate, respectively.Statistics. Treatment effects of the bioassay were analyzed by analysis of variance (ANOVA) with a randomized complete block design. Mean separation was done using the least significant difference test (LSD).Charcoal additions increased biomass production of a rice crop by 17% in comparison to a control on a Xanthic Ferralsol (Figure 1). This increase was largely an effect of improved P, K, and possibly Cu nutrition. Nitrogen and Mg uptake decreased in charcoal amended soils which resembled the uptake pattern of rice grown on an Amazonian dark earth (Fimic Anthrosol; Figure 1). Charcoal additions had no significant effects on S, Ca, Fe, Zn, and Mn uptake (P>0.05). In addition to charcoal, fertilization was necessary with N, S, Ca, and Mg for optimizing rice growth. The soil fertility improvement of the dark earth was largely an effect of enhanced P, Ca, and micronutrient availability such as Mn and Cu. Crop nutrition of S and K was not better and that of N and Mg was even lower in rice grown on a dark earth in comparison to the Ferralsol. Fertilization was necessary for those elements and was effective in increasing total nutrient uptake (Figure 1).Figure 1: Biomass production and nutrient uptake by rice (Oryza sativa) after additions of charcoal and fertilizer to a Xanthic Ferralsol or a Fimic Anthrosol after 37 days (means and standard errors; N=4). Therefore, charcoal directly amended the soil with plant-available nutrients such as P, K, and Cu. If fertilizer was applied together with the charcoal some nutrients showed a higher uptake efficiency than the added effects of fertilization and charcoal amendment would suggest. This was the case for N, Ca, and Mg. In the following we discuss the reasons for a higher efficiency of a combined application.Under the high leaching conditions in upland soils of the central Amazon, reduction of nutrient losses by leaching is an important aim in order to improve nutrient availability for plants. Immediately after fertilizer application, nutrient contents significantly increased as shown for ammonium (Figure 2) and leveled off to background levels only 21 days after fertilization. This was also the case with K, Ca, and Mg (data not shown). Leaching from the unfertilized Ferralsol was reduced when charcoal was applied and resembled the low values found in the Anthrosol (Figure 2). Ammonium concentrations in the leachate were also significantly lower in the fertilized Ferralsol after charcoal applications. These results indicate that ammonium was adsorbed by the charcoal and elevated N uptake by rice after the combined application of charcoal and fertilizer (Figure 1) was an effect of ammonium retention. This retention could not be found for other cations or anions, because K, Ca, and Mg were in higher supply with charcoal additions. After several cropping cycles, the nutrients in the charcoal may be depleted and results may differ from those shown here. Since N was applied as ammonium, nitrate contents in the leachate were controlled by biological transformation rather than physical adsorption. In accordance with the leaching results, only ammonium was adsorbed by charcoal (Fig. 3) whereas all other nutrients (P as PO 4 2-, Ca, Mg, K) showed higher concentrations in the equilibrium solution than added (data not shown). The process of adsorption is largely a co-adsorption with soluble organic matter, as an addition of dissolved organic carbon (DOC) from a manure extract increased ammonium adsorption. A microbial immobilization or nitrification during shaking can be excluded, since the adsorption was similar when microbial activity was suppressed by additions of azide (Fig. 3). If a slash-and-char technique was to be successful, (i) the quantity of applied charcoal must be produced from the same area of land that is cropped, and (ii) the periods of charcoal production must at least correspond to those of land clearing practiced so far. In other words, the slash-and-char technique must work with the same resources as conventional methods and be an alternative to slash-and-burn or slash-and-mulch. The amount of charcoal which can be produced from different forest vegetation primarily depends on the woody biomass available, and additionally on the production procedure such as charring environment (e.g., oxygen), temperature and time (e.g., Glaser et al., 2002). The average recovery of charcoal mass from woody biomass is 31% according to the published data compiled in Table 1. The effect of different charcoal production methods on its recovery in agricultural fields is not well known and the charring environment such as temperature and charring time is usually poorly documented. The carbon contents of charcoal do not vary much and lie around 63-83% with a mean of 76% (Table 1). The carbon recovery from charred woody biomass is relatively high with 54% (Table 1) due to the high carbon contents of charcoal.Several published values of above ground biomass from secondary and primary forests in the central Amazon show a high proportion of woody biomass (Table 2). Biomass of secondary forests increase with age but depend largely on site conditions and previous land use. Larger amounts of charcoal can be produced from primary (57-66 Mg C ha -1 ) than secondary forests calculated with the average conversion from Table 1. But also secondary forests may produce charcoal equivalents of up to 32 Mg C ha -1 after only 4 years (Table 2). The pot experiment shown in Figures 1 and 2 was conducted with a charcoal amount of 135 Mg C ha -1 (20% weight in 10 cm depth), but also 67 Mg C ha -1 (10%) were shown to significantly improve biomass production of cowpea (Lehmann et al., unpublished). In a pot experiment with Inga edulis, tree height and stem diameter significantly increased through the addition of charcoal (Figure 4; ANOVA P=0.041 and 0.007, respectively). Already at the lowest application rate (13.3 Mg C ha -1 ), charcoal additions were equivalent to fertilizer applications. Therefore, the charcoal amounts produced from the same area of land which is used for cropping during one charring event are sufficient for improving crop performance and for reducing nutrient leaching. Lower amounts of 7.9 Mg C ha -1 were shown to have only minor effects on rice yield in the first cropping season under field conditions (Steiner and Nehls, unpublished data) but more information is needed from field experiments. With increasing charcoal additions, growth of Inga decreased when no fertilizer was applied but increased with fertilizer applications. Charred organic matter from leaves was not accounted for in the calculation and the conversion to charcoal is currently not known. The contribution of leaves to charred organic matter from secondary or primary forests may be small, however, since the proportion of leaves in these forests usually lies below 10% (Table 2). Nevertheless the contribution to total nutrient input may be significant and has to be considered in nutrient budgets Charcoal applications directly increased nutrient availability such as P and K and additionally increased nutrient retention for ammonium. Whether a net nutrient retention of other cations occurs after excess nutrients have been leached or taken up by plants remains to be shown. In this respect the longterm dynamics of soil fertility with charcoal applications are very interesting in comparison to burning or mulching. It may be assumed that nutrients bound to charcoal are more persistent than those in ash or mulch but direct evidence needs to be gathered.The Eastern plains (Llanos) of Colombia cover an area of 26 million ha and are the only land in Colombia available for agricultural expansion. The climate is sub-tropical with distinct wet and dry seasons. The natural landscape is a mosaic of grass covered, rolling savanna dissected by numerous rivers and streams that are bordered by evergreen gallery forest. Small termite mounds are abundant in the grasslands. The gallery forest accounts for nearly 10% of the total area (Rippstein and Girard, 1995). Low soil fertility and frequent natural or induced fire prevent forest from re-colonizing the land (Rippstein et al., 2001). Over the past two centuries the native grassland has been grazed by low productivity livestock and currently supports 3 million cattle (Rivas, 2000).The soils of the Llanos are very acid (pH <4.3) Oxisols (Typic Haplustox), low in nutrients and very high in aluminum saturation (>80%). There are two contrasting textural groups; approximately 90% of the soils are clay-loams and 10% are sandy-loams. The soils are generally poorly drained and prone to compaction that can be induced by tillage or by cattle trampling (Amézquita, 1998). Despite these limitations, agricultural activities have intensified in the region during the last decade. Oil palm, rice, maize, soybeans, sorghum and improved pastures are now appearing, especially in the vicinity of the main cities and connecting roads. Intensification will be accelerated in the coming decades and various crops and management strategies have been developed to allow sustainable use of the Llanos (Friesen et al., 1997). The most promising among these are improved pastures with mixtures of grasses (e.g, Brachiaria sp.) and forage legumes (e.g. Desmodium ovalifolium, Arachis pintoi, Centrosema acutifolium), and rotations of cowpea or soybeans as green manures with varieties of upland rice or acid tolerant maize (Friesen et al., 1997b).The Llanos is a complex ecosystem from the perspective of exchange of greenhouse gases between the land and the atmosphere, and agricultural intensification can be expected to have a large impact on these exchanges. In the natural system, fire and termites cause emissions of CO 2 , CO, CH 4 , NO, N 2 O and volatile organic compounds to the atmosphere (Delmas, 1997;). Cattle are important sources of CH 4 and forage quality has a large impact on the amount of CH 4 emitted per unit of live weight gain or milk production. Soils are sources or sinks for carbon, N 2 O and CH 4 . Improved pastures have shown major increases in carbon stocks in soils of the Llanos (Fisher et al., 1994). Several studies in temperate ecosystems have found that the conversion of natural lands to agriculture may reduce oxidation rates of CH 4 and/or increase emissions of N 2 O by soils (Sitaula et al., 1995;Mosier et al., 1991;Davidson et al., 1995, Willison et al., 1995). High doses of N-fertilizers stimulate N 2 O emission but can reduce CH 4 oxidation (Bronson and Mosier, 1994). Less information is available for soils of the tropics; Nobre (1995) found that the conversion of native Cerrados in Brazil to high input agriculture increased N 2 O emissions by a factor of ten relative to natural environments. On the other hand, Lauren et al. (1995) found little impact of land conversion to pasture or cropland on CH 4 and N 2 O fluxes from a soil near Brasilia. Soils at this site were net sinks for atmospheric CH 4 throughout the year and oxidized from three to six times more CH 4 than similar land uses in temperate regions. Emission rates of N 2 O were very low compared to temperate system counterparts. Venezuelan savannas are believed to be small net sources of CH 4 , although only short-term measurements have been reported (Sanhueza et al., 1995).Changing land use in the Llanos will influence more that one of the factors responsible for exchange of greenhouse gases with the atmosphere. Conversion of savannas to pastures or cropland eliminates fire and reduces the population of termites, thereby reducing emissions of CO 2 , CO, CH 4 , NO, N 2 O and volatile organic compounds. Improved pastures will enhance carbon sequestration in soils and the higher quality forage will reduce emissions of CH 4 per unit of production. However, improved pastures also allow higher stocking rates, leading to increases in CH 4 emission by cattle per unit area of land. Conversion of land to cropland is expected to reduce the soil CH 4 sink strength and increase N 2 O emissions. The objectives of this study were to assess annual fluxes of N 2 O and CH 4 for the main land uses found in the Llanos, to use this and other data to make estimations of the radiative forcing potential (Global Warming Potential (GWP)) of this ecosystem under current conditions and to predict the effect of expected changes in land use over the next two decades on regional GWP. Results can be used to inform policy makers on decisions affecting the future development of the region.Field research was conducted at the Corpoica-CIAT Carimagua station, in the middle of the Colombian Llanos (4° 37' N latitude; 71° 19' longitude). The altitude is 175 masl, and annual rainfall and mean temperature were 2498 mm and 28°C respectively for the period of the study (Nov 1997to Dec 1998). Oxisols of two contrasting textures, a clay-loam (18% sand, 47% silt, 35% clay) and a sandy-loam (64% sand, 17% silt, 19% clay), typical of the Llanos were used in this study.Six study sites representing native and agricultural systems were chosen. Four sites were selected from treatments in a long-term agro-pastoral/crop rotation experiment that was established on a clay loam soil in 1992 to evaluate the sustainability of several cropping and management systems in the Llanos. Details of the experiment are presented elsewhere (Friesen, 1996). The systems used were: native savanna, legume-grass pasture (B. dictyoneura + A. pintoi), upland rice as a monocrop and upland rice in a rotation with cowpea as green manure. Experimental plots were strips 20-m wide by 200-m long. A native savanna site on a sandy-loam soil (30-km southwest of the main site) and gallery forest site (9 km to the north of the main site) on a clay loam soil were also studied.A detailed characterization of soil physical and chemical parameters to a depth of 30 cm was performed in May 1998. Bulk density was measured by the sand replacement method and resistance to penetration by a cone penetrometer. Both parameters were measured using procedures described by Smith and Mullins (1991). Four replicate undisturbed core samples were taken at 5-cm depth increments for determination of air permeability following the method of Koorevaar (1983). Samples were collected in metal cylinders (5-cm diameter, 5-cm height) that were capped immediately after collection. Saturated hydraulic conductivity, particle size distribution, soil porosity and pore size distribution were measured as described by Smith and Mullins (1991) and susceptibility to uniaxial soil compaction as described by Culley (1993). Chemical parameters measured were pH (water 1:1), soil organic matter (wet combustion), N content (micro Kjeldahl), and available P (Bray II). Chemical analyses followed the procedures of Hendershot et al. (1991). In April and October 1998, levels of nitrate and ammonium were determined in samples collected down to 1-m depth.The vented closed chamber technique (Conen and Smith, 1998;IAEA, 1992) was used to monitor fluxes of CH 4 and N 2 O between the soil and the atmosphere. Four replicate PVC rings (10-cm high by 30cm diameter) per treatment were permanently inserted 7-cm into the soil. White polyethylene vented chambers (10 cm high and 30 cm diameter) were attached to the rings just prior to each 1-hour measurement period. A 5 cm wide rubber band cut from a tire innertube was used to seal the joint between the chamber and the ring. The top of a chamber was fitted with a septum to facilitate extraction of gas samples, a hole for insertion of a digital thermometer, and a venting glass tube to prevent pressure differentials between the chamber and the atmosphere. The dimensions of the venting tube (0.5 cm diameter, 8 cm high) were selected as described by Hutchinson and Mosier (1981), and the tube was inserted 5 cm inside the chamber.To reduce temperature increases within chambers, a reflecting white cover was placed over the chamber during the period of sampling. Gas samples were collected in 20 mL teflon-valved glass syringes at 0, 20, 40 and 60 minutes after installing the chamber. Immediately after collection, 15 mL of gas were transferred to high vacuum pre-evacuated glass containers (10 mL in volume). The glass containers were pre-evacuated to 2-3x10 -6 mbar, using a freeze dryer that also allowed capping of the evacuated vials with butyl rubber caps, which are impermeable to CH 4 and N 2 O (IAEA, 1992). An aluminum over-cap was crimped over the rubber cap to effectively seal the flask and avoid accidental opening. Preservation of samples in these containers was found to be longer than 6 months, however analysis of samples was always completed within four weeks of collection.At the time of gas sampling, composite soil samples (0-10 cm) were collected from around each chamber for determination of moisture content. Soil temperature was measured within the area of the chamber just before and after the sampling period. Air temperature inside the chamber was recorded at every sampling interval to later account for the change in the density of the air inside the chamber as a function of temperature. Gas flux measurements were initiated on November 1997. Sampling frequency was at three-week intervals, except for the first month when three samplings were made. The order and time of sampling was standardized; sampling was started at 8 am, 9:30 am, 11 am and 2:00 pm for the sites under clay-loam savanna, rice, rice-cowpea and pasture respectively. Samples from the sandy-loam savanna and the gallery forest were collected the next day beginning at 10 am and 3 pm respectively.A Shimadzu model 14A gas chromatograph (GC) equipped with FID and ECD detectors was set up to simultaneously analyze CH 4 , N 2 O and CO 2 in the same sample, while venting N 2 , O 2 and H 2 O (Rondón, 2000). A 14 port, two-position valve (Valco Instruments) was used to inject samples via a 2-mL gas loop and to re-direct the stream of gas to the ECD detector just after the CH 4 peak was obtained with the FID detector. The system used a pre-column (1-m) and a main column (3-m), both were 1/8 inch stainless steel tubing filled with Porapaq Q (80-100 mesh), and a carrier gas (N 2 ) flow rate of 25 mL min -1 . An electrically operated pneumatic actuator was used to precisely time the switching of the valve. CLASS VP software (Shimadzu) was used to control the actuator as well as the GC. The GC temperatures were 24°C at injection port, 60°C for the columns and 320°C for the ECD detector. For each gas sample, a 3 mL sub-sample was withdrawn from the glass vials using a 5 mL glass syringe fitted with a Teflon valve and injected into the GC via the sample loop. The existence of positive pressure inside the vials was checked at the time of sample withdrawal. Analysis time per sample was 5.5 minutes. Gas retention times were 2.1 minutes for CH 4 , 3.8 min for CO 2 and 4.8 minutes for N 2 O. Class VP software was used to calculate the concentrations (ppm) of the gases relative to the standards. Compressed air and Scotty prepared mixtures containing 1 and 3 ppm CH 4 and 0.9 and 5 ppm N 2 O were the most commonly used standards. For samples high in CH 4 or N 2 O, other standards were used (i.e. 10, 100 ppm CH 4 and 10, 100 ppm N 2 O) as appropriate.Differences in gas concentrations over time were used to calculate gas flux to/from soil for each chamber using the condition of a linear increase for at least three points to accept a flux measurement. When the linearity condition did not occur, the four samples were reanalyzed and the chamber was not considered in the calculations of flux averages if the problem persisted. For CH 4 , fluxes are shown as net oxidation rates (net CH 4 consumption by the soil) with emissions from soil shown as negative values. For N 2 O, positive fluxes represent emissions from soil. Annual fluxes were estimated by integrating the net area under the curve for the plot of gas flux over time. The method was applied to each of the four chambers in every land use, in order to calculate the reported average value. Annual fluxes represent the balance between periods of net emission and net consumption.Comparisons of annual flux averages between land-uses were made by one-way analysis of variance using SYSTAT version 8 software. Comparison of means was done using Tukey's HSD method. A level of significance of ≤5% was used.Weekly rainfall and minimum, maximum and average temperatures for the period of measurement are shown in Figure 1. Annual rainfall in the study year (2323 mm) was slightly higher than the 20-year average for the station (2150 mm). March was also wetter than usual while September was drier than usual. In general, the Llanos has a dry season from November to April and a wet season from May to October. A clear alternation of dry and wet days is common in the rainy season, which helps to prevent the soil from becoming waterlogged for long periods of time. The number of days without rainfall (222) exceeded that of rainy days (142) during the year. Towards the end of the dry season, strong solar radiation and frequent winds dried grassy vegetation. Deep rooted shrubs and the gallery forest, with the ability to extract water from deep in the soil, remained green throughout the year. Temperatures are fairly constant through the year with a maximum daily variation of 10°C. For most of the day, temperature remains in a narrow band from 26-29°C. No significant correlation was observed between air temperature and gas fluxes. With the exception of the gallery forest soil, saturated hydraulic conductivity was generally low in the clay loam savanna soils, which explains the characteristic wetness of this environment. Poor drainage was exacerbated by conversion of savanna to pasture. Reduced water conductivity in the pasture (Figure 3C) is attributed to sub-surface soil compaction as can be seen from the increased resistance to penetration in the 5-10 cm depth (Figure 3J), probably as a result of cattle trampling. In fact, susceptibility to compaction (Figure 3F) is higher for the whole profile in the pasture compared to the corresponding savanna. Lower susceptibility values for the 5 to 10 cm and 20 to 25 cm layers of the pasture indicate that these layers are already compacted. Other researchers (Mosier and Delgado, 1997;Keller et al., 1993) have also reported subsurface compaction and increased water retention in pastures. As expected, hydraulic conductivity (Figure 3C) is higher in the sandy soils than in the clay-loam soils, demonstrating the influence of soil texture. The greatest hydraulic conductivity was found in the gallery forest soils, probably as a result of improved aggregation due to higher SOM contents, coupled with increased presence of root channels and faunal activity which, in turn, results in low soil bulk density values.Methane oxidation rates from November 1997 to December 1998 for soils under native vegetation and agricultural use are shown if Figures 4A and B, respectively. The temporal variation in CH 4 oxidation rate is high, with average values ranging from +120 µg CH 4 m -2 h -1 for the forest in December to -320 µg CH 4 m -2 h -1 (net emission) for pastures, in August. Spatial variability within a land use was also high, with RSD values ranging from 10% to 400%. However, for most of the sampling dates, RSD was in the range 60 to 120%, which is comparable to other studies on similar soils in the tropics (Poth et al., 1995;Cofman et al., 1998;Scharfe et al., 1990;Keller, 1994). The highest spatial variability was found with soils under rice, while forest and sandy savanna soils showed consistently lower variability.Methane fluxes followed distinct patterns according to rainfall and soil moisture regimes. During the dry season (November to April), soils under all land uses were net sinks for atmospheric CH 4 . Oxidation of CH 4 was progressively reduced as the soils became wetter in the rainy season (May to October). Soils of the clay loam savanna and the pasture eventually became a source of CH 4 during the period of peak rainfall (Figure 1) when soils were at their wettest (Figure 2).The gallery forest soil consistently had higher CH 4 oxidation rates than the other ecosystems and none of the four sampling areas were ever a source of CH 4 . Low bulk density (Figure 3A), high water infiltration rates (Figure 3C), high air permeabilities (Figure 3I) and low resistance to penetration (Figure 3J) are physical attributes of soil in the gallery forest that explain these results. In contrast, all other land uses on the clay loam soil have lower values than the gallery forest soil for air permeability and hydraulic conductivity through the 30 cm sampling depth. The clay loam savanna and pasture soils showed the most physical constraints to drainage and gas transport in the top 10cm of soil, consistent with these two systems becoming a source of CH 4 during the wet season. The pasture soil had the highest water filled pore space (Figure 2B) and the highest CH 4 emissions of all systems during the month of July. Converting the clay loam savanna into cropland, reduced CH 4 oxidation rates during the dry season (Figure 4A and B), probably as a result of reduced availability of water. On the other hand, tillage improved drainage and aeration, reducing the length of time that agricultural soils become anaerobic, which results in only small emissions from these soils in the wet season.Soil texture was an important factor affecting CH 4 fluxes in the two savanna sites. Methane oxidation rate in the sandy savanna soil was lower than that in the clay loam savanna during the dry season, probably because low water retention (Figure 2) reduced the population and/or activity of CH 4oxidizing bacteria (Boekx et al., 1997;Bottner 1985) relative to the clay loam savanna soil. The greater total porosity, and especially macro-porosity (Figure 3F Estimates of annual CH 4 fluxes are presented in Figure 5A. The gallery forest soil, with a sink strength of 3.05 kg CH 4 ha -1 y -1 , constituted the highest sink for CH 4 . The CH 4 sink strength in the gallery forest is similar to values reported for a wet forest in Puerto Rico (Steudler et al., 1991), and other types of tropical forest in Central and South America (Keller, 1994;Keller and Reiners, 1994;Keller and Wofsy, 1986). The annual CH 4 sink strengths in the sandy and clay loam savanna soils were only about one-third and one-twelth of that of the gallery forest soil, respectively. Methane sink strength in the Llanos clayloam savanna soil (0.26 kg CH 4 ha -1 y -1 ) is similar to values reported for grasslands on Oxisols of Puerto Rico (Mosier and Delgado, 1997), and higher than values reported by Sanhueza et al. (1995) for comparable savannas in Venezuela, which were found to be a minor source of CH 4 . However, methane sink strength on well aerated, high clay Oxisols of the Brazilian savanna (Cerrados) was 1 to 2 times higher than that of the Llanos savannas (Lauren et al., 1995). y -1 for rice mono-cropping and by 4x to y kg CH 4 ha -1 y -1 for the rice-cowpea rotation These results contrast with several reports (Sitaula et al., 1995;Mosier et al., 1991, Bronson andMosier, 1994) showing a decrease in methane consumption rate when soils were fertilized with ammonium fertilizer sources. (However, information to specifically address effects of N status/source (fertilizer or green manure) on CH 4 oxidation capacity was not collected in the present study. Presumably, removal of physical constraints to gas exchange counteracted any negative effect of fertilizer on CH 4 oxidation rate.Conversion of clay loam savanna to pasture changed the soil from a net sink to a net source (1.92 kg CH 4 ha -1 y -1 ) of CH 4 . A similar result was found for conversion of tropical forest in Costa Rica to pasture (Keller, 1983). Several other pastures sites in temperate (Van der Pohl, 1999) and tropical regions (Mosier and Delgado, 1997) have been found to be net sources of atmospheric CH 4 . Keller and Reiners (1994) found that the condition of pastures greatly affected CH 4 emissions, with emissions from abandoned, degraded pastures being nearly five times higher than from pastures in good condition. The pasture plots used in the present study were 7-years old, originally planted with B. dictyoneura and A. pintoi, and supporting a stocking rate of 3 head/ha. Signs of pasture degradation were evident at time of sampling: low persistence of the legume, increased bulk density, subsurface compaction, reduced air permeability, occurrence of isolated termite mounds etc. Options are available to improve the management of these pastures in ways that reduce soil compaction and prevent or slow pasture degradation (Amezquita, 1998).Fluxes of N 2 O from soils of the native and agricultural systems over the one-year study period are shown in Figures 6a and 6b, respectively. Spatial variability in N 2 O emissions was higher than that for CH 4 , with RSD values mostly in the range of 100 to 200%, although values as high as 400% were sometimes found. This level of variability is similar to that found in other studies (Williams et al., 1999;Ruser et al., 1998;Veldkamp et al., 1998;Keller and Reiners, 1994). Soils of all land-uses were net sources of N 2 O for most of the year, although small sink strengths occurred in the sandy soil savanna at the end of the dry season and in the pasture soil at the end of the rains. Emission rates from natural ecosystem soils were generally low, with the gallery forest the main contributor. A peak of emissions was approximately coincident with the peak of the rainy season in the gallery forest. No consistent difference was found between the savannas on soils of contrasting texture. Observed low emission rates in native land uses may be partially the result of the small amount of nitrogen that is cycled in these nutrient-limited soils (Figure 7) and low soil pH, which reduces both nitrification and denitrification rates (Broadbent et al., 1965;Jackson, 1967). Emission rates for the savannas are similar to those reported for the Brazilian Cerrados (Nobre, 1995;Lauren et al., 1995) and for native grassland bordering the Guyana rain forest in Venezuela (Sanhueza et al., 1990), but were higher than those reported for the more comparable savannas of Venezuela (Hao et al., 1988).Conversion of clay loam savanna to a grass-legume pasture did not significantly increase N 2 O emissions although legumes are known to do this (Galbally et al., 1992;Duxbury et al., 1982). A possible reason is the relatively low proportion of legume in the pasture studied. The annual emission value of 1.27 kg is lower than values for temperate grasslands (Williams, 1999;Ball et al., 1997a;Van den Pohl, 1999) or from fertilized pastures in Costa Rica (Veldkamp et al., 1997), but falls within the range found for degraded pastures in Costa Rica (Keller and Reiners, 1994), and Puerto Rico (Mosier and Delgado, 1997).Annual emissions of N 2 O were significantly increased (p<0.01) by slightly more than three-fold when clay loam savanna was converted to cropland. There was no difference in emissions between the two rice systems (Figure 8), where N is supplied predominantly through inorganic fertilizer in the monocrop and via organic sources in the rotation. This result does not support the suggestion (Freney, 1997), that more N 2 O is emitted from organic than inorganic N sources. The observed emission rates in the rice systems of 2.8-3.0 kg N 2 O ha -1 y -1 correspond to 1.8% of external nitrogen inputs after removing the contribution of the background flux from the savanna soil. This proportion is higher than the value of 1.25% x fertilizer-N which has been frequently used as an average for fertilized fields (Mosier et al., 1995), but lower than the more recently suggested proportion of 1.25% x fertilizer-N + 1 (Hopkins et al., 1997). The data is similar to other studies on tropical acid Oxisols (Mosier et al., 1998), where the conversion of the native ecosystem to agriculture, with additions of 100kg N as fertilizer, resulted in a five-fold increase in N 2 O emissions from soil.The role of the soil Table 1 shows figures for the area of the various land uses found in the Llanos, as well as their estimated contribution to annual net fluxes of methane and nitrous oxide. Data for coverage area corresponds to the nearly 14 Mha of the so called well-drained savanna which is more suitable for intensification of agriculture or pastures (Rivas, 2000).By contributing about half of the total methane sink, soil in the gallery forest plays a key role in the net balances of this trace gas in soils of the Llanos. Given the relatively small area covered with forest, any disruption could have an important impact on regional soil methane sink strength. To illustrate this, if current forest area were reduced by 50%, the net methane sink by soils from the Llanos would be also reduced by 50%. Crops included in this study are not the only ones currently used or likely to be used in the future, and therefore a degree of uncertainty arises in regional gas budgets when it is assumed (as in Table 1) that rice as a monocrop and in rotation with green manure are representative of the effect of cropland in the region. However, given that the area under crops is still small, their potential contribution to changes in balances of methane in the Llanos is probably not too high, even under the scenario of a two fold increase in cropland expected for the next two decades (Smith at al., 1997). The annual sink strength of methane in soils from the Llanos (0.0078 Tg/y) represents around 0.02% of the estimated 40 Tg/y global soil sink strength (Minami, 1997).Total N 2 O emissions are greatly controlled by the native land uses (savannas and gallery forest), due to their high area coverage. Crops and pastures contribute currently in similar proportions to the overall budget of this gas in the Llanos. The global annual emission is low (0.1%) with respect to the estimated global planetary emissions of 13Tg/y (Bowman, 1994). At least two types of termites are found in soils of the Llanos: subterranean soil-feeding termites and mound building termites (Decaëns, 1995). In a study conducted simultaneously with this, Rondón (2000) has shown that essentially all methane generated by subterranean termites is oxidized by soils before escaping into the atmosphere. The only contribution to net emissions of this gas is made by species of mound building termites of the genus Spinitermes. Annual estimated fluxes due to termites were reported as 6.7 and 7.2 g CH 4 /ha for pastures of B. humidicola and native savanna respectively (Rondón, 2000). These values are fairly low compared to net soil sinks in the region, and consequently methane emissions by termites does not constitute an important component in the budgets of this gas in the Llanos. Soils under other land uses did not have termite mounds. To extrapolate annual fluxes of methane from termite mounds to the overall area of the Llanos covered with native savanna (in clay and sandy soils) and with pastures, it has been assumed that the density of 36 active mounds per hectare reported by Rondón (2000), for native savannas and 26 for pastures, applies throughout the Llanos. The integrated annual methane flux coming from termite mounds in the Llanos is 76 Mg CH 4 /year. This value is only about 0.0004% of the total global emissions of 19.7 Tg CH 4 attributed to termites (Sanderson, 1996).Towards the end of the dry season, vegetation in the savanna becomes too dry to be of value for cattle. To favor the re-growth of higher quality grasses, ranchers frequently burn their savannas. This, in addition to common natural fires results in a complete burning of the savannas at least every two years. Burning affects fluxes of GHG by two mechanisms: direct emission to the atmosphere in the form of the products of combustion, and indirect effects created by disturbances of the normal fluxes of gases from the soils. In a parallel study, Rondón (2000) measured both direct emissions of methane and nitrous oxide and long-term effects of burning on soil-atmosphere exchange. Extrapolating annual emissions to the area of the Llanos which is susceptible to burning (9.96 million hectares), direct emissions of methane due to burning were estimated to be 67,728 Mg CH 4 /y, while indirect effects represent a reduction in 723 ton CH 4 /y in the soil sink capacity (Rondón, 2000). The net annual release of methane by burning is then 68451 Mg per year, which is nearly 11 times higher than total methane oxidation by soils. Burning consequently has a major role in the annual budget of methane in the Llanos. The combined release of nitrous oxide in the region due to burning is 6928 Mg N 2 O/year, which is about 37% of the total emissions of this gas by soils in the region. Its contribution is then also important though not dominant in the regional balance of nitrous oxide.Methane emission by cattle is a well-documented process, believed to be responsible for annual emissions on the order of 90 Tg (Johnson, 1996) or approximately half of total agricultural sources (Cole et al., 1997). Unfortunately there is a complete lack of data regarding methane emission by cattle grazing native savanna vegetation or improved pastures in the Llanos. Cattle population in the well drained Llanos is estimated to be around 2.5 million animals (Fedegan, 2000). Kurihara et al., (1999) reported methane emissions by cattle fed on tropical grasses of the order of 113g CH 4 /cow-day. Though grasses are different, assuming the same methane production rate for cattle in the Llanos, the estimated production of methane by cattle in the region would be approximately 103,000 ton/year. This is about 16 times higher than the net sink by soils. This demonstrates the key role of cattle in controlling the budget of methane in the Llanos.The total number of cattle in the Llanos is not expected to increase significantly in the near future due to market and demand constraints. What is expected with the introduction of improved pastures, is that cattle will move from native savannas frequently in remote locations, to improved pastures near to the roads and infrastructure. There are probably good opportunities to improve balances of methane in the region by offering better quality forage for the cattle. Future research in the Llanos should in consequence, try to account for the effect of improved diets on local methane budgets.In Table 2 results from the contribution of the major components of gaseous exchange are presented, and extrapolated to the respective area of influence to generate total annual fluxes for the Llanos. Data in Table 2 indicates that all together, the savanna ecosystem constitutes a net source of atmospheric methane, being largely controlled by direct emissions generated by burning and by the unfortunate 'bad breath\" of cattle. The Llanos emits only about 0.03% of the total estimated global annual emissions of methane (535Tg, IPCC, 1997), and about 0.1% of total annual emissions of nitrous oxide. Covering an area of approximately 0.094% of the planetary land area, the region shows emissions of N 2 O similar to planetary averages, while emission of CH 4 is only around one third of average global emissions. The Llanos can consequently be labeled as an environmentally friendly ecosystem. Despite that, there are opportunities to further reduce emissions of GHG's in the region. 1). (♣) An average stocking rate of 0.6 heads/ha was assumed for the Llanos (Rivas, 2000) (^)Termite mounds were considered for the soils under savannas and pastures.Data in Table 2 indicates that mitigation strategies should be directed towards reducing the frequency of fires and reducing emissions by ruminants. Probably there is a little opportunity to favorably alter emission factors by burning at other times during the dry season, this impact has to be evaluated. Burning is however very important for maintaining the productivity and functioning of the ecosystem and also to permit the current economic exploitation of the savannas.Therefore, unless more profitable management options are offered to farmers, there is little opportunity to reduce the scope of fires in the savannas. Pastures could play a role here, as they are economically feasible options for the development of the region (Vera, 1997). As was mentioned before, improved pastures with mixtures of high productivity grasses and forage legumes could also play a role in reducing emissions of methane by animals in the region.In Table 3, the combined effect of all components on the balances of methane and nitrous oxide has been integrated to provide annual emissions per unit area in each land use. Though pasture soils were found to be a net source of methane, the fact that burning is eliminated in well managed pastures counteracts emissions by soil. However, given that stocking rate is increased six fold when converting a unit area of savannas into pastures, there is a 4.6 fold increase in the net release of methane to the atmosphere per unit area due to the cattle. Taking all the factors into consideration, conversion of savannas to cropland is the only alternative identified in this study, which can convert the savanna ecosystem into a net sink of methane, by eliminating the sources (burning and termites) and enhancing the soil sink. This option would however increase net emissions of nitrous oxide and consequently a \"compromise solution\" should be adopted when trying to include the environmental perspective within the development programs for the Llanos. It is clear however, that under the low fertilizer application rates expected to be used in the Llanos, crops will provide a good alternative to the development of the region in an \"environmentally friendly way\".Clearly, regenerating forest on deforested land will provide the best alternative to mitigate emission of greenhouse gases in the Llanos. Unfortunately this is not an option easy to implement, because fire normally prevents the advance of forest into the savanna. This implies that measures to prevent the fire from reaching the borders of the forest should be reinforced, but that normally involves high cost in building roads which the farmers will not be able to afford if they are only for the sake of the environmental benefit. One possibility to cope with this problem would be to foster the use of areas near the borders of the forest as pastures or croplands. It is however reasonably to expect that governmental subsidies should be employed to make this option feasible. Perhaps there is an opportunity to recruit some funds by selling the equivalent GHG offset resulting from recovering forest (Moffat, 1997). Soil carbon, a solid component in the greenhouse gas analysis Soils of the Llanos have been found to be able to sequester important amounts of atmospheric carbon when deep rooted grasses are introduced in these lands. Net C sequestration by pastures of Brachiaria humidicola in the top 1m deep soil was reported as 25.9 ton C in a ten year period, while grass-legume pastures of B. humidicola and the forage legume Arachis pintoi increased such amounts to 70.4 ton/10 years (Fisher et al., 1994). Though in this study fluxes of CO 2 were not considered, It is clear that the extent of the reported carbon accumulation in soils under pastures plays a main role in configuring the complete scenario of GHG's in the Llanos. Consequently, an analysis will be attempted here to include this component.As a mechanism for integrating the combined effect of all greenhouse gases involved in the Llanos, the CO 2 -equivalent global warming potential (E-GWP) of CO 2 , CH 4 and N 2 O has been calculated for two time horizon scenarios (20 and 100 years), for every land use in the Llanos. In a 20-year time scenario, CH 4 has a GWP equivalent to 62 times that of CO 2 , while that of N 2 O is 275 times compared to CO 2 . In the 100 years time horizon, the corresponding GWP values for CH 4 and N 2 O are respectively 23 and 296 times that of CO 2 (IPCC, 2001). The calculation of integrated E-GWP expressed as equivalent kg of CO 2 , was done by multiplying the per hectare annual emission of CO 2 , CH 4 and N 2 O from each contributing factor, by the land area associated with that factor and then by the relative GWP of each gas. Adding together the values obtained for each factor gives the overall equivalent E-GWP for the Llanos, expressed as equivalent units of CO 2 . For the calculation, it was assumed that burning does not make a net contribution to emissions of CO 2, because the CO2 released by fire is reabsorbed from atmosphere during vegetation regrowth. Stocking rate of cattle was assumed as 0.5 head/ha in clay and sandy savannas and 3 head/ha in pastures. The same CH 4 emission factor for cattle was used for improved pastures and for native savannas. Soil emissions of CH 4 and N 2 O were assumed to be the same in grass alone and in grasslegume pastures. Figure 9 shows calculated E-GWP values (for one year total emissions of all GHG's) on a hectare basis for the various land uses and has been calculated for two time horizons, 20 and 100 years of influence. Figure 9 includes the reported (Fisher et al., 1994) values for carbon sequestration in pastures of grass-legume (0.1Mha) with a high rate of carbon sequestration (70.4 ton C/ha in a 10-year period), as well as pastures of grass alone (0.5Mha) with lower rate of carbon accumulation (25.6 ton C/ha in a 10year period). Annual carbon sequestration by pastures was calculated assuming the same rate of accumulation for each year, and then converting it into CO 2.All natural land uses (savannas and forests) show positive equivalent E-GWP values, indicating that they are contributing to the radiative forcing of the atmosphere. The gallery forest is clearly the best natural land use from the perspective of the heating effect on the atmosphere. Its equivalent E-GWP is very low in both time scenarios. At the 20-year horizon, the warming contribution from the gases emitted during one year by one hectare of forest is equivalent to that of the CO 2 emitted by the combustion of 35 gallons of gasoline (fuel for 8 hours trip of a small car!). Including all the sources and sinks, the radiative power of savannas is low and decreases when the 100 year time scenario is used, because most of the contribution is in the form of CH 4 , which is a short lived gas in the atmosphere. Crops have integrated E-GWP lower than that of the savannas in the 20 year scenario and approximately the same as the savannas in the 100 year scenario. The conversion of savanna land into cropland does not have a detrimental effect on the E-GWP. The inclusion of pastures in the Llanos plays a much more important role in affecting the overall E-GWP. Due to the modest emissions of methane and nitrous oxide from pastures and the very high sequestration of atmospheric CO 2 as soil organic carbon, pastures can convert the system from a modest source into an important net sink of radiatively important species. The rate and persistence of C accumulation plays a major role in the strength of the sink, especially in the 20-year horizon. Even with rates of C accumulation in soil of around half of the reported value for grass alone pastures in Carimagua, the equivalent E-GWP of annual emissions from one hectare of pastures would be zero.Land use patterns in the Llanos are expected to change in the next two decades. Studies suggest (Smith et al., 1997;Rivas, 1999), that the area of crops could increase up to two times the current values while the area under pastures will also double in the same period. The area under grass-legume pastures is expected to grow from 0.1 to 0.3 Mha, while the area under grass alone pasture will continue to dominate and will increase from 0.5 to 0.9 Mha. This expansion will be at the expenses of the clay-loam savanna, which has slightly better levels of soil nutrients than the sandy savanna. The area under gallery forest will probably decrease by 10% in the next 20 years assuming the same rate of current intervention. Annual rate of C-sequestration by pastures in soil was assumed as reported for grass alone and grass-legume pastures at Carimagua (Fisher et al., 1994).Table 4 shows results of a calculation of the integrated E-GWP for the Llanos at present and in the year 2020 for a 20-year time horizon. Under current land use distribution, the Llanos as a whole plays a minor role in the radiative forcing in the earth's atmosphere. Its integrated E-GWP of 9.6 Tg of CO 2 equivalents is only about 0.004% of estimated global planetary radiative contribution of about 242,000 Tg of CO 2 equivalents (IPCC, 2001). In Figure 10, in addition to the 20-year time horizon a longer term 100-year time horizon has been used to calculate effects of present and expected land use distribution in the Llanos. The development of the Llanos will have small net benefits to the environment by reducing the radiative force of the atmosphere. This benefit will be accentuated in the longer term scenario. Once more the minor role of the Llanos in the context of warming of the planet is emphasized. This study presents the first data set on fluxes of methane and nitrous oxide for the Colombian Llanos. Results indicate that gallery forest is an important sink for atmospheric methane, while savannas are a minor sink. Therefore, preservation of the gallery forest should be of priority concern as this environment also provides a home for a large biodiversity of endemic plants and animals.Conversion of soils into cropland does not reduce their methane oxidizing capacity and some of the management practices could even increase their sink strength. This may be the result of eliminating some of the physical constraints that limit the gas exchange between soil and the atmosphere (soil compaction, surface sealing etc.). On the other hand it also increases emissions of nitrous oxide and is equally expected to increase losses of soil carbon as a result of tillage. Despite this, given that the main contributing factors (burning and cattle) are excluded in cropland, it can be anticipated that agriculture will be a better option than savanna for reducing the radiative forcing of the Llanos.Emission of methane by biomass burning is a key factor in the balance of this gas in the Llanos. Any action that can reduce the area submitted to burning and or the frequency of burning events, will improve the radiative balance in the region. In this respect, conversion of savanna into croplands or pastures is clearly an advantage because burning is eliminated in such land uses. Promoting the recolonization of the land by gallery forests constitute a win-win situation as it will not only eliminate the burning, but also will increase the methane soil sink strength.Fire plays a key role in maintaining the biodiversity in the savanna, in addition to other important though not fully understood ecological roles. Consequently, complete suppression of the burning is not a desirable option. Appropriate corridors to maintain the continuity of the savanna ecosystem should always be considered.Cattle-associated emissions of methane dominate methane budgets in the Llanos. Improving estimates of their actual contribution as well as exploring promising opportunities to reduce their impact by offering forages of higher nutritional value, are important topics for research in the near future.Natural ecosystems as well as converted lands constitute small net sinks for N 2 O in the Llanos. Emission rates are related with the amounts of nitrogen cycled in the soil; nitrogen inputs in the form of fertilizer or green manure cause enhanced emissions. Strategies to manage these inputs in order to minimize nutrient losses and reduce environmental impact require further attention.In general fluxes of methane and nitrous oxide from soils in the Llanos can be considered low, but fall within the range reported for similar environments in Africa (Seiler et al., 1984), Central America (Mosier et al., 1998), Brazil (Lauren et al., 1995) and Venezuela (Scharffe et al., 1980), and even for tall grass prairies in temperate regions (Tate and Striegl, 1993).Though pastures will increase methane emissions from cattle due to the increase in the stocking rate as compared to savanna, by avoiding the fire and by sequestering atmospheric CO 2 in the form of soil organic carbon, pasture is the only land use option identified in this study, that can shift the land from a net source into a net sink of atmospheric GHG's.This study has shown that the Llanos are only a very minor contributor to the warming of the atmosphere and that expected intensification of agriculture and cattle production in the coming two decades would not have negative effects on the radiative forcing potential of the region. Despite this, there are other well identified constraints for the sustainability of the natural resource base, whose impact should never be forgotten. Pasture degradation is major cause of pasture abandonment specially in the Brazilian Cerrados and the Amazon. Degradation could result not only in reduced C sequestration in soils but even turn them into net sources of Carbon (Da Silva et al., 2000). Though current pasture degradation is not too severe in the Llanos, unless appropriate management practices were adopted, this could become a critical problem in the region, whose environmental consequences are still to be evaluated.In the jargon of optometrics, 20-20 means perfect vision. We hope that appropriate vision will be used by policy makers in the design of development plans for the Llanos which allows the region to continue being an environmentally friendly ecosystem in the year 2020.The vicious cycle of deforestation: Vast areas of the Amazon rainforest have been cleared in the last decades to be converted into pastures. After few years of use, the land is degraded as a result of nutrient depletion, soil compaction and surface sealing. Productivity declines severely and pastures become abandoned, giving place for a succession to secondary forest. A new forest area is then usually cleared to start the process again. Abandoned lands are characterized by very low storage of nutrients and reduced stocks of soil organic carbon (SOC). Some alternatives do exist to recuperate degraded land including the establishment of agroforestry systems. These options are expected to help in restoring soil nutrients and allow C sequestration in both biomass and soils. This poster presents information on C storage in soils under 10 year old agroforestry systems and secondary vegetation, as well as on primary forest for an area in the Central Amazon.The Charcoal contribution. Natural and anthropogenic fires are frequent in the Amazon forest. Combustion of plant material is never complete specially for roots, and this result in variable amounts of residual charcoal being added to the soils. Charcoal is ubiquitous in Amazonian soils and is present in a range of size particles: from coarse (>2 mm diameter) usually found mostly at the soil top layers, to very fine particles (<50 µm) distributed along the soil profile.Charcoal is mainly carbon in an extremely inert form but until now not much effort has being devoted to define this as a separate C pool in soils. Given its inherently heterogeneous distribution, charcoal presence creates problems when trying to assess the effect of a given land management option on C sequestration by soils. Changes of SOC are normally small for short to medium terms and could be masked by the \"noise signal\" created by the charcoal. This noise also confounds interpretation of the dynamics of SOC when the 13 C technique is used (Desjardins et al., 1996). To be able to separate any difference in SOC resulting from different land use, charcoal contribution to total soil C has to be assessed. In this study we evaluated the contribution of different charcoal size classes to total soil C.The project is being conducted at EMBRAPA-CPAA research station at km 54 north of Manaus, Brazil. In a long-term experiment, four alternatives to recover degraded land in the Central Amazon have been studied: establishment of two silvopastoral (SPS) and two agroforestry (AFS) systems: SPS included ASP1 (medium fertilizer inputs) combining Brachiaria brizantha, Desmodium ovalifolium and mahogany (Sweithenia macrophyla) and low input ASP2 in which B. Brizantha has been replaced by B. humidicola. AFS were: AS1 based on palm species(Bactris gasipaes and Euterpe olearaceae) and also includes Cupuaçu (Theobroma grandiflorum and Colubrina acreanaea ). AS2 is based in native and exotic fruit trees (6 species) and also includes Mahogany and Brazil nut (Bertholletia excelsa). Secondary vegetation of similar age to the AFS and primary forest soils have been used as controls. Three repetitions of every system and control were evaluated. The area has a mean annual rainfall of 2250 mm and average temperature of 28°C. Soils are high clay Oxisols, very low in fertility.Given the complexity of the AFS studied, to obtain a representative sample of soil from a given systems is a complex task. Soil sampling was based on a species-interaction strategy. In each plot (3000 m 2 ), a composite soil sample of five sites was taken from each of the main plant-plant interaction found in the plot. Samples were separated at 0-5, 5-15, 15-30, 30-60 and 60-100 cm depth. Soil was air dried and gently dissagretated to <4mm diameter. A type Jones sample divider was used to separate soil in three size classes: 2-4 mm, 0,5-2mm and less than 0,5 mm diameter. Charcoal was separated by hand in a subsample of the 2-4 mm (G) and 0.5-2mm (M) size. Then, the original sample (free from medium to large size charcoal) was reconstituted to be analyzed for total C and nutrients. In a subset of samples, the fine fraction was used to determine black carbon content using the methodology of Kuhlbusch (1995). Finely ground soil (<53 um) was oxidized with repeated doses of NaOH (four times), HCl, HNO 3 , H 2 O 2 , H 2 O and finally thermally oxidized in a Oxygen-rich environment at 350C for 3 hours. C content in the original sample and the final residue were determined in a dry combustion CHN analyzer. C content in the residual soil after the chemical and thermal oxidation is defined as black carbon, and in addition to charcoal it includes the most highly resistant components of SOM.Charcoal. Figure 1 shows the relative contribution from charcoal in each size class to total charcoal for the case of the ASP1 system. Charcoal in medium and large sizes is more abundant in the surface and subsurface layers and decays strongly as soil gets deeper. It is worth nothing however, that medium size particles are found even at 1m depth suggesting or localized burning of deep roots or migration of charcoal from the surface probably through root or soil fauna channels. Variability in charcoal content in the M and G size classes was high indicating non-homogeneous distribution of charcoal in soils. The contribution of the fine fraction is much more homogeneously distributed through the soil profile. Soil C stocks. There were found significant differences among C stocks in most soil layers except in the surface layer where variability was the highest. Figure 3 shows the C stocks stored in the 60-100 cm depth soil layer. Carbon storage at that layer was higher in the primary forest soil as compared to other systems, being followed by the fruit based system (which includes some large trees), suggesting an important contribution from deep roots to C buildup. The ASP2 low input system presented the lowest C storage at such depth. This is in agreement with lower aerial biomass estimates for such system reported by McCaffery (Poster in this meeting). In Figure 4, total C storage in the soil profile is presented for all systems (data correspond to C content after removing the coarse and medium size charcoal fragments). Significant differences were found between systems. When the charcoal contribution is taken into account, such differences become hidden. Forest soils store the highest amount of C (121 Mg.ha -1 ), followed by the AS2 system with 116 Mg.ha -1 . On the other extreme, soils under secondary vegetation and ASP2 system presented the lowest stock (106 Mg.ha -1 ). Results in this study indicate that agroforestry systems permit a moderate recovery of soil C stock relative to the control under secondary vegetation. Rates of C accrual are in the order of 1.8 Mg.ha -1 .y -1 . This contrasts with much higher rates reported for temperate and tropical regions (Bruce et al., 1999). It is worth mentioning however, that the initial soils were highly degraded after supporting cattle grazing for more that 12 years. Application of fertilizer was also very low to the systems. This suggests that there is space for increasing C accumulation rates through the practice of agroforestry. Charcoal is an ubiquitous constituent of the soils in the Central Amazon and appreciable amounts can be found even at 1 m depth. Coarse fragments are located preferentially at the surface layers showing high heterogeneity in its spatial distribution. Fine particles distribute rather homogeneously through the soil profile. Charcoal derived-C can account for as much as 15% of total soil C. Separation of coarse and medium size charcoal fragments is very important to allow appropriate comparison between SOC in different land use systems in areas where fire is a factor in the natural or human influenced management of the forest. Although charcoal separation and the assessment of black carbon is a time consuming process, given that charcoal is a remarkably stable pool, once a baseline has been established for a certain site, the same information could be used in future studies.Though various studies have shown that soils under pastures enable high rates of C sequestration (Fisher et al., 1994) in our sites, the lack of fertilizer inputs and the high initial degradation of the land prevented a significant accrual of SOC as compared to soils under secondary vegetation.Even under unfavorable initial conditions, agroforestry systems allow net C accumulation in soils, permitting the soils to move in a 10 years time period, from 87% to 95% of the total C stocks in the primary forest. The assumed relationship between biodiversity or local richness and the persistence of 'ecosystem services' (such as sustained productivity and regulation of water flow and storage) in agricultural landscapes has generated considerable interest and a range of experimental approaches, but the abstraction level aimed for may be too high to yield meaningful results. Many of the experiments on which evidence in favour or otherwise are based are artificial and do not support the bold generalizations to other spatial and temporal scales that are often made. Future investigations should utilise co-evolved communities, be structured to investigate the distinct roles of clearly defined functional groups, separate the effects of between-and within-group diversity and be conducted over a range of stress and disturbance situations. An integral part of agricultural intensification at the plot level is the deliberate reduction of diversity. This does not necessarily result in impairment of ecosystem services of direct relevance to the land user unless the hypothesised diversity-function threshold is breached by elimination of a key functional group or species. Key functions may also be substituted with petro-chemical energy in order to achieve perceived efficiencies in the production of specific goods. This can result in the maintenance of ecosystem services of importance to agricultural production at levels of biodiversity below the assumed 'functional threshold'. However it can also result in impairment of other services and under some conditions the delinking of the diversity-function relationship. Avoidance of these effects or attempts to restore nonessential ecosystem services are only likely to be made by land-users at the plot scale if direct economic benefit can be thereby achieved. At the plot and farm scales biodiversity is unlikely to be maintained for purposes other than those of direct use or 'utilitarian' benefits and often at levels lower than those necessary for maintenance of many ecosystem services. The exceptions may be traditional systems where intrinsic or 'non-use' values continue to provide reasons for diversity maintenance. High levels of biodiversity in managed landscapes are more likely to be maintained for reasons of intrinsic ('non-use'), serependic or 'option' values or utilitarian (direct use') than for functional or 'indirect use' values. The major opportunity for both maintaining ecosystem services and biodiversity outside conservation areas lies in promoting diversity of land use in ways that meet these requirements at the landscape scale. This requires however an economic and policy climate that favours diversification in land-use products and diversity among land users.The role of biological diversity in the provision of ecosystem goods and services and the way this role can be valued and managed during agricultural intensification is much debated but still poorly understood. A key problem in all debates on biological diversity is that the abstraction 'diversity' has often not been distinguished from the specific attributes of the community of organisms that is under study in any particular location or system For instance if the interest lies in the functional roles of the community these may depend on the 'structure' of the vegetation and the relationships between different 'functional groups', rather than on diversity as such. Experiments based on random species assemblages may be appropriate tests for hypotheses about 'diversity' per se, but tell us very little about the largely selfselected assemblages that make up natural ecosystems. In the case of agro-ecosystems, whilst the dominant crops or livestock are artificial, by far the majority of the species are self-selected. So, are we asking the right question? Does the loss of diversity at plot-to-global scales imply a threat to critical ecosystem functions? Can we identify thresholds in such a process? Global diversity derives from the lack of overlap in species, genetic or agro-ecosystem composition between geographic or temporal domains as embodied in the niche concept. While 'agricultural development' affects local (ie. plot level) diversity, it probably has even stronger effects by homogenizing at higher scales, facilitating the movement of 'invasive species' and the introduction and spread of 'superior' germplasm of desirable species. Scale is thus of overriding importance in our analysis and we may well find that answers may appear contradictory between different ways of defining temporal and spatial boundaries to the system under consideration. In this review we will first consider the concepts of 'biodiversity' and 'ecosystem functions', and then the evidence that links relevant aspects of the two, before we embark on an exploration of how this relationship depends on scale and can be 'managed'.Humans have evolved as part of the world's ecosystems, depending on them for food and other products and for a range of functions that support our existence. Natural ecosystems, as well as those modified by humans, provide many services and goods that are essential for humankind (Matson et al., 1997). Efforts and interventions to manipulate (agro)ecosystems to meet specific production functions, represent costs to the rest of the ecosystem in terms of energy, matter and biological diversity, and often negatively affects goods and services that so far were considered to be free and abundant. These are anthropocentrically regarded as services because they provide the biophysical necessities for human life or otherwise contribute to human welfare (UNEP, 1995;Costanza et al., 1997). Most if not all of these services are based on a 'lateral flow', or movement across the landscape of biomass (such as food, fibre and medicinal products derived from the sea, inland waters or lands outside of the domesticated 'agricultural' domain), living organisms and their genes, or earth (nutrients), water, fire or air elements. Examples of ecosystem services particularly important for agroecosystems and agricultural landscapes are: maintenance of the genetic diversity essential for successful crop and animal breeding; nutrient cycles; biological control of pests and diseases; erosion control and sediment retention; and water regulation. At a global scale other services become important such as the regulation of the gaseous composition of the atmosphere and thence of the climate. A list of such services is given in the first column of Table 1 of the Appendix, and their connection to lateral flows is discussed by Van Noordwijk et al (Table x, this volume).These ecosystem goods and services are biologically generated. The community of living organisms within any given ecosystem carries out a very diverse range of biochemical and biophysical processes that can also affect neighbouring systems. These can be described at scales ranging from the subcellular through the whole organism and species populations to the aggregative effect of these at the level of the ecosystem (Mooney and Schultze 1993). All ecosystems have permeable boundaries with respect to material exchanges but the within-system flows usually dominate those between systems, such as between land-use or land-cover types within a landscape. For purpose of this paper we define ecosystem functions as the minimum aggregated set of processes (including biochemical, biophysical and biological ones) that ensure the biological productivity, organisational integrity and perpetuation of the ecosystem. There are no agreed criteria for defining a minimum set of such functions but for the purposes of this paper the second column of Table 1 lists ecosystem functions alongside the ecosystem services they provide. Further explanation of these relationships is given below but it is useful to note that these functions can be pictured as having a hierarchical relationship. The energy captured in primary production is utilised in the herbivore and decomposer food chains. Interactions between these three subsystems occur through nutrient exchanges and a variety of biotic regulatory mechanisms as well as by energy flow. In particular the balance between the constituent processes of primary production and those of decomposition determines the amount of energy and carbon maintained within the system and is the major natural regulator of the gaseous composition of the atmosphere at a global scale (see Swift 1999).Most discussions and empirical studies on biodiversity have focused on issues of a relatively small range of organisms. In contrast, the Convention on Biological Diversity defines its area of concern as: \" …the variability among living organisms from all sources, including, inter alia, terrestrial, marine and other aquatic ecosystems and the ecological complexes of which they are part; this includes diversity within species, between species, and of ecosystems\" (Heywood and Bates, 1995). Diversity within each one of these three fundamental and hierarchically related levels of biological organisation can be further elaborated as follows: genetic diversity is the variation within and between species populations; species diversity refers to species richness, that is, the number of species in a site, habitat, ecological zone or at global scale; ecosystem diversity means the diversity of assemblages (and their environments) over a defined landscape, ecological zone or at global scale.Biodiversity in this paper refers to the totality of the species (including the genetic variation represented in the species populations) across the full range of terrestrial organisms i.e. invertebrate animals, protists, bacteria and fungi, above-and below-ground, as well as the vertebrates and plants which often constitute the main concerns of biodiversity conservation. With a definition as broad and inclusive as this, it is highly unlikely that any clear and precise statements about relationships between 'biodiversity' and functions can be formulated and tested that can be helpful in guiding human activity. Similar to the situation with 'watershed functions' we may find that discussions on components of the overall biodiversity concept in relation to land use are more productive and open to progress than those that stay at the aggregate level. In the section immediately following we shall refer to the diversity within ecosystems (often termed alpha diversity) and in later sections to that at the broader scale of the landscape (which embraces concepts of both beta and gamma diversity).The analysis of biodiversity and its management is highly influenced by the perspective used. In particular different sectors of society attribute different values to biodiversity. Broadly speaking four different types of value can be usefully recognised, although different terminology is often used..First is the intrinsic or 'non-use' value of diversity to humans, which comprises its cultural, social, aesthetic, and ethical benefits. Some groups in society attribute high social and religious values to individual species or communities of organisms; others derive value from the simple fact of high diversity per se in such systems as tropical rainforests or coral reefs. Second is the utilitarian or direct use value of components of biodiversity, i.e. the subsistence, financial and monetary benefits of species or their genes derived by one or other sectors in society. The direct use value may be private and accrue to the land managers (farmers, local community, government). This is most obvious with respect to high value agricultural crops but also applies to the other types of good listed in Table 1. For instance, the pharmaceutical industry values the tropical forest tree Prunus africana very highly because its bark contains chemicals used for manufacturing a drug. Another example is that in Africa, many farmers living near natural (and protected) forests withdraw substantial monetary benefits from their hunting and from collecting plants and tree products in these forests (Pottinger and Burley, 1992).Thirdly, biodiversity can be said to have serependic or 'option' value. This is the belief in future but yet unknown value of biodiversity to future generations, for example the presence of a microorganism with an as-yet undiscovered genetic potential for industrial products. These three types of value of biodiversity are ethnocentric and depend very much upon the cultural values and preferences of different sectors of society. This is why some authors, interested in such values, stress that 'the conservation of biological diversity depends as much on society's ethical views as on facts' (Barrett 1993).Finally, biodiversity and the integrity of processes that maintain and generate diversity have functional significance. This may be the 'indirect use ' value of Kerry Turner (1999). Part of this functional significance may be of direct utilitarian value for Homo sapiens in the production of goods and services that can be priced. Beyond this lie a range of ecosystem services that are of acknowledged benefit to humans but which generally lie outside the boundaries of recognised utilitarian benefit. The purpose of this paper is to analyse the functional values of biodiversity with particualr reference to the diversity in agricultural landscapes but it also has a more general significance for life over and beyond this single species. This non-human significance, however, can only be translated into a 'value' that can play a role in human decisions via human 'champions' and thus cannot in practice be separated from the intrinsic value.Biologists have for many decades speculated on the question of why there are so many species of living organism. As explored in the theory of island biogeography, the diversity within any ecosystem at any point in time is the result of a 'self selection' process, that involves co-evolution of the species comprising the biological community within a given ecosystem by interactions among them and with the abiotic environment through time. This is not an isolated process. New species may enter an ecosystem from neighbouring areas, some establishing themselves and others failing to do so. Partly as a result of successful newcomers or new adaptations emerging in existing ones (be they competitors, predators, pests or diseases), and partly as a result of fluctuations in abiotic environmental conditions, some of the existing species may become (locally) extinct over any period of time. The species richness of any given ecosystem or land unit is therefore a dynamic property. In agro-ecosystems farmers take a dominant role in this dynamic by the selection of which organisms are present, by modifying the abiotic environment and by interventions aimed at regulating the populations of specific organisms ('weeds', 'pests', 'diseases' and their vectors and alternate hosts). The dynamic nature of the (local, patch level) diversity of any system, whether natural or agricultural, is often underrated, as is the importance of the selection pressure and process. The diversity of any system is not adequately represented simply by the number of species (or genotypes) present, but by the relationships between them in space and time. Attempts to assemble combinations of the same number of species under slightly different conditions and in particular without the history of interaction often fail (Ewel et al 1991). But what makes any existing species combination into a 'system' is still largely elusive. Some insights obtained in analysing food webs may help. For example Neutel (2001) showed that the majority of belowground food webs constructed from random combinations of organisms did not meet dynamic stability criteria, even though all parameters such as abundance of groups and dynamic properties were chosen in a 'normal' range when considered one-byone. Yet, systems with the actual parameter combinations that are attained in the field do [DID?] meet stability criteria, suggesting that partly uncovered rules about the proportionalities and co-variance within the normal range are crucial.Debate on the relationship between biological diversity and ecosystem function has a long history which has taken on new vigour (and sometimes even rancour) since the advent of the Convention on Biological Diversity (see Woodwell and Smith 1969 for the older literature and Schulze and Mooney 1993, Mooney et al 1995, 1996, and many of the citations below for more recent discussion). Vitousek and Hooper (1993) contributed a major focus to this debate through hypothesising three different possible relationships between plant diversity and broad-based ecosystem functions such as the rate of primary production (Figure 1). Their analysis of current evidence led them to propose that the asymptotic relationship shown as Curve 2 in Figure 1 was the correct one. This suggests that whilst the essential functions of an ecosystem, such as primary production, require a minimal level of diversity to maximise efficiency this effect is saturated at a relatively low number. Swift and Anderson (1993) proposed that this relationship could also apply to the decomposer system. Examples of essential functions in this case are the basic suite of catabolic enzymes (e.g. for cellulolysis, lignin degradation etc), the facilitation role that invertebrates play by reducing particle size by their feeding activity, and biophysical processes of pore formation and particle aggregation. It is interesting to note however that the communiies of organisms contributing to the ecosystem function of decomposition are taxonomically much more diverse than those of primary production.Over recent years a number of authors have reported on experiments investigating the links between diversity and specific functions (e.g. see Ewel et al 1991, Naeem et al 1994, Naeem and Li 1997;Tilman and Downing 1994;Tilman et al 1996Tilman et al ,1997;;Hooper and Vitousek 1997) that appear to broadly corroborate the predictions of the Vitousek-Hooper hypothesis for primary production. This has however generated an equal amount of discussion in refutation and the issue remains significantly a matter of interpretation and opinion (see Grime 1997;Hodgson et al 1998;Lawton et al 1998;Wardle 2000;Naeem 2000). There is no space here to review these studies in detail. Each one of the experiments quoted can be criticised in one way or another. The strictest interpretation of many of the experiments would be that the conclusions apply only to the specific combinations of organisms used in the tests, and in most cases these are assemblages constructed for experimental purposes rather than naturally co-evolved communities. At a fundamental level such experiments suffer from a basic methodological paradox -in order to describe and understand diversity and complexity we need to simplify it, and take away the self-selection that governs real-world diversity. Dealing with the totality is impossible. For instance there is no single (or combination of) methods that would allow for the total inventory of the species richness of even a small volume of soil. It is thus difficult to draw general conclusions about 'diversity' as such and in particular with respect to naturally co-evolved communities. The results of such 'un-natural' experiments may however be more applicable to agricultural systems that in one sense can be said to have been assembled in a similar way.The minimum diversity required within a functional group One potentially valuable interpretation of the Vitousek-Hooper relationship has been that the minimal level of diversity required to maximise the production function consists of representatives of an essential set of 'functional groups' of plants. A functional group may be defined ' a set of species that have similar effects on a specific ecosystem-level biogeochemical process'. As Vitousek and Hooper put it the 'essential' plant species are those that contribute in different ways to the key ecosystem functions -in the case of primary production by exploiting different components of the available resources by differences in canopy structure to maximise light capture or symbionts and root architecture to optimise capture of water and nutrients. Drawing together the threads of this discussion we [hypothesise that?] suggest that 'the minimum diversity essential to maintain any given ecosystem function can be represented by one or a few functionally distinct species i.e. one or a few representatives of a small range of functional groups' is a useful null-hypothesis to guide investigations of the functional significance of biological diversity in agricultural systems. It may need further operationalization for specific ecosystem contexts, however. The total diversity required then depends on the number of functions that are recognized and to the degree of overlap in 'functional groups' between these different functions.The functional group concept is briefly discussed in the Appendix to this paper and Table 1 lists a minimal set that we propose are needed to provide the ecosystem goods and services we have been addressing.The classification of plants into functional groups has drawn a great deal of recent attention because of the recognition of the pressure being exerted on terrestrial ecosystems by global climate change (Smith et al 2000) The primary producers (together with the vertebrate herbivores) are our major source of food and are also the source of fibre and other useful materials such as latex. Molecules with antibiotic, therapeutic, pesticidal or similar biological activities utilised by humans are however synthesised by many groups of organisms (e.g. bacteria and fungi) and are often very specific in origin. Diversity is therefore an essential pre-requisite for maintenance of supply, particularly of new products, although the capacity to biologically generate or synthesise new compounds under laboratory conditions has been greatly increased by the advent of genetic engineering.Decomposition and mineralisation of organic matter of plant and animal origin and synthesis and decomposition of soil organic matter are carried out by a very diverse community of invertebrates, protists, bacteria and fungi. Other elemental transformations often are carried out by a diverse set of functional groups with very specific biochemical capacities, for example certain of the bacteria of the nitrogen cycle. Diversity within these groups varies from very low to high, but it can be experimentally demonstrated that a single species per function may be sufficient under a given set of environmental conditions.The dominant biological properties regulating water flow and storage in the soil are the plant cover, the soil organic matter content and soil biological activity. Macrofauna such as earthworms, termites and It is valuable to note that these are not necessarily mutually exclusive hypotheses, as they may refer to different space and/or time aspects of the system and the function of specific concern. We need to clearly separate the question of how the current diversity came into being (the 'self organization' of the system, based on the success in the evolutionary history of all component species) from the human or teleological perspective of the relevance of this diversity. Just as we have to distinguish between 'diversity per se' and 'diversity of actual systems', we have also to recognize that not all components of a system have the same probability of being lost as a result of simplification of agro-ecosystems and some functions may therefore be more resilient than others. Differences in life histories of the key groups of organisms confer different temporal and spatial contexts to their role in the ecosystem and their responsiveness to its self-organising properties.The third of Vandermeer et al's (1998) hypotheses is extremely pertinent to the question of how much of this diversity is needed to maintain ecosystem goods and services in the face of agricultural intensification and other aspects of ongoing 'global change'. There is certainly substantial experimental evidence that the many key functions can be maintained by only small numbers of species within a particular functional group. For example monotypic cover by perennial plants can be as effective as a diverse community in controlling erosion. Although the decomposer community of a particular soil may be very diverse only a minority of the hundreds of species of fungi, bacteria or invertebrates participate in the decomposition process at a given time and place. The extent of redundancy implied by this can be demonstrated under laboratory conditions where decomposition can be fully mediated by single species cultures of enzymatically-diverse organisms such as white-rot basidiomycete fungi whilst in nature the same process may be carried out by several species of fungi, bacteria and animals (Swift 1976, Giller et al 1997).The third hypothesis raises questions whether key functions can be maintained by one (and the same) species under all circumstances. This addresses the issue of the capacity of ecosystems to adapt to changing circumstances that result from elements of stress and disturbance. The capacity of a system to respond to and recover from disturbance is termed its resilience. This property has been attributed to the degree of connectivity within an ecosystem, a feature that depends at least in part on the composition and diversity (Holling 1973(Holling , 1986;;Allen and Starr 1982). Diversity within functional groups may provide an important means for increasing the probability that ecosystem performance can be maintained or regained in the face of changing conditions. For the below-ground community for instance there is evidence that the same enzymatic function is carried out by different species of bacteria or fungi from the same soil under different, and even fluctuating, conditions of moisture stress or pH (see Griffin 1972 for discussion of this). In the case of plants different species may play a similar functional role in different seasons, under varying conditions of climatic or edaphic stress and in different stages of patch-level succession.Functional diversity thresholds are thus likely to be higher in the real world than in the relatively controlled situations under which most of the experiments on diversity-function relationships have been conducted. Recognition of the importance of diversity to the property of resilience suggests furthermore that the implication of equilibrium in the way that Figure 1 is drawn (see also figures 2 and 3) may be misleading. The shifts between different states of functional efficiency with changes in diversity are more likely to be rather abrupt. Perhaps a case could be made recognising resilience as an ecosystem service rather than a property. An alternative view, however, is to see resilience as a property which varies among functions rather than a unitary ecosystem property. The decomposition function for example, may be substantially more resilient than that of the regulation of specific pest populations.Resilience is a concept that requires consideration of different spatial scales. The resilience of any local system after shocks that lead to local loss of diversity depends strongly on the ability of organisms to recolonize from the neighbourhood, and thus on the distance to the nearest suitable habitat and the dispersal of the organisms in question.What is the impact of agricultural intensification on biodiversity and ecosystem functions? Our main concern in this paper is with biodiversity issues in agricultural landscapes i.e. landscapes containing agroecosystems. Agroecosystems can be defined as (natural) ecosystems that have been deliberately simplified by people for purpose of the production of specific goods of value to humans. The simplification down to one or a few productive plant or animal species is implemented for greater ease of management and specialisation of product to suit market demands, especially in highly mechanized forms of agriculture. In an ecological sense the system may be seen as one which is maintained by a high frequency of disturbance, in an early successional stage (Conway, 1993). In such systems a distinction has been made between 'planned' and 'associated' diversity (Swift et al 1996;GCTE 1997). The planned diversity is the suite of plants and livestock deliberately retained, imported and managed by the farmer. The composition and diversity of this component strongly influences the nature of the associated biotaplant, animal and microbial. The issue is more complex than the single issue of the extent of planned biodiversity that is maintained however. Agroecosystems are managed by substitution and supplementation of many of the natural ecosystem functions by human labour and/or by petro-chemical energy or its products. In addition to their direct effects on production these interventions provide the means to reduce the risk associated with reliance on ecosystem services, although it can be argued that this is serving to substitute one set of risks for another -that of dependence on the market. Furthermore whilst substitutions may buffer some of the functions they also run the risk of further damaging others. For instance the addition of pesticides may control diseases of immediate negative impact but also kill nontarget organisms with other functions such as pollination or soil fertility enhancement.During agricultural intensification the diversity of crops and livestock is reduced to one or a very few species of usually genetically homogenous species. The varieties are selected or bred for yield (e.g. high plant harvest index), taste and nutritional quality. Plant arrangement is commonly in rows, fallow periods are bare, sequences may be monospecific (varietal) or of two or rarely more species. This is in contrast to natural ecosystems where the genetic diversity of plants (both within and among functional groups) is high but varies in relation to environment. The effects of land use change and agricultural intensification on biodiversity and associated functions are still poorly understood but conversion to agriculture almost always results in fewer species of both planned and associated biota with lower genetic variation and representing less functional groups. Nonetheless the extent of diversity in even so-called monocultures may be underestimated by plot-level assessment of diversity at any point in time. A rapid interannual turnover of the germplasm is often employed to stay ahead of the evolutionary race with pests and diseases, adding a time dimension to diversity that may exceed evolution in natural systems, albeit with respect to a narrow genetic base. This varietal turnover depends however on 'externalized' functions of maintaining genetic diversity in gene banks, and on the mechanisms of rapid multiplication and transfer of such germplasm. This situation contrasts with that of extensive agricultural systems where diversity is deliberately maintained within the system with or without external exchange. Here a plot-level assessment may have more relevant boundaries of measurement, although lateral flows of organisms exist here as well. Production systems based on perennial crops and trees provide less opportunity for rapid turnover of varieties for obvious reasons, and there clearly is a much stronger need here for maintaining plot-level diversity as a risk management strategy (Van Noordwijk and Ong, 1999).Whilst many recent experiments have tended to confirm that community primary production may be maximised by a low-number diversity of functional types (see above) there is also abundant evidence that mono-typic stands can reach the same levels of production within relatively narrow environmental conditions. Biomass production is however not the only function or service performed by plants in ecosystems. The secondary functions related to ecosystem services may be more biodiversity-sensitive than that of food production. 'Intensive' production systems for specific high-value products (e.g. spices) can however be very diverse. Another exception may be in relation to pharmaceutical and agro-chemical goods. Most products of these types are initially gathered from natural or secondary vegetation or derived from microbial cultures obtained from soil. Once the markets for such products are established, however, the required control over the concentrations of biologically active substances, and the opportunities for monopolization tend to favour more technically advanced modes of production. Maintaining global diversity is thus essential for both present and future needs although the synthetic capacity brought by the molecular biological revolution is fast rendering this less so. Herbivore diversity is highest in heterogeneous systems with high plant and resource diversity but monotypic vertebrate herds can reach equivalent levels of production in simplified grazing systems. Pest epidemics tend to occur in circumstances of low genetic diversity of the host plants or livestock.Nutrient cycles become more open in agricultural systems with losses of nutrient through offtake in harvest, run-off from compact surfaces, increased volatilisation through a changed surface environment and increased leaching associated with decreased soil organic matter content. These losses can be substituted by inorganic inputs but the efficiency of return to the plant is often low and fertilisation is usually required at levels far in excess of direct crop demand, which further exacerbates the losses and can leads to pollution of groundwater etc. There is substantial evidence demonstrating gains in crop productivity from nutrient additions through mixtures of organic and inorganic sources of nutrients compared with either alone (e.g. Swift et al 1994). Maintenance of organic inputs to the soil is thus an important management strategy for efficient use of external inputs. Advantages in utilising a variety of such inputs have also been demonstrated because of the strong influence of input chemistry (''resource quality') on patterns of mineralisation. The diversity of organisms involved in nutrient cycling may be substantially reduced under agricultural intensification but there is little evidence of significant effects on decomposition and mineralisation processes which has been attributed to a high level of functional redundancy among decomposer fungi, bacteria and microregulators such as nematodes or collembola (e.g. see Beare et al 1997, Giller et al 1997). The significance of this loss of diversity should not however be assumed to be inconsequential. In particular it is unclear how the resilience of the system under conditions of change is influenced by such loss. Organisms with very specific functions, such as those exhibited by some bacteria of the nitrogen cycle, often show specialisation to particular soil conditions such as pH and specific genotypes may be lost as a result of soil degradation. Specific strains of dinitrogen-fixing bacteria may also be lost a result of agricultural intensification resulting in the need for subsequent inoculation (Kahindi et al 1997).Soil organic matter (SOM) is a keystone component of the ecosystem in the sense that its impact on overall system performance exceeds its relative share in the energy flow through the system. Soil organic matter (SOM) stores and buffers nutrient concentrations, influences water storage in the soil and is a major factor in determining soil structure and thence erosivity. Above all it is a store of energy in the soil that drives many of the soil-based processes. SOM synthesis and decomposition is brought about by much the same community of organisms as those involved in decomposition of plant litter. A well-charted phenomenon is the decline in SOM as a result of conversion of natural ecosystems to agriculture. Farmers utilize the nutrients mineralised as part of this decline of the SOM capital to support high initial levels of crop production after clearance. Soil tillage is also an effective additional way of stimulating the breakdown of SOM and plays a key role in promoting crop yields after land conversion to agriculture, until a new and lower equilibrium between breakdown and formation of SOM is reached. The level of the new SOM equilibrium, with its consequent impact on nutrient cycling, soil water regimes and erosivity, is related to the quantity of plant litter input, which is almost invariably lower than that of natural systems. Crops in intensive systems are usually selected for high harvest indices, and there may be uses for crop residues other than soil fertility maintenance (e.g. fodder or fuel). The SOM content is thus related to the quantity, diversity and mode of management of organic input to soil. A key feature of agroecosystem management is thus the trade-off between the gains in production from 'mining' the SOM versus the potential negative impact on its other ecosystem services and in particular on system resilience. This 'trade-off' between the different values of SOM has been rarely recognised but become a matter of greater interest as society has begun to realize the potential value of sequestering carbon in soil as a means to slow down the rate of global climate change. A research question of continuing interest is whether the functional properties of SOM are in any way influenced by the diversity of organic materials from which it is synthesised.The most important factors regulating water infiltration and retention are the extent of ground cover by plants and/or plant litter. The reduction in these, including interposing of periods when ground is bare, leads to greater run-off and diminished infiltration as well as increasing the risk of erosion. Substitution by mechanical tillage can ameliorate as well as aggravate these effects. Monospecific cover can be just as effective as a divers one with respect to limiting run-off and erosion, trapping sediment and promoting infiltration, but to be effective it has to be present year round. Diversity of organic inputs is likely to have a positive effect by widening the probability of differences in timing of litterfall and rates of disappearance from the soil surface. As soil protection on slopes depends more on partially decomposed litter with good ground contact than on fresh leaves that can be easily washed away, the role of plant diversity on slopes is likely to be greater than on flat lands. The macrofauna moving between litter layer and soil strongly influence partitioning of water between surface runoff and infiltration as well as modifying water movement within soil. Interesting examples of the influence of these 'ecosystem engineers' show how circumstance specific diversity effects may be. Soil engineers making macropores in the soil are not welcome in all circumstances. In bunded rice fields, farmers make an effort to destroy soil structure by puddling to reduce the porosity of the soil and building dykes to contain the water. These earthworks may be destroyed by the actions of earthworms and surveys by Joshi et al. (1999) in the Ifugao Rice Terraces (IRT), in the Philippines showed that 125 out of 150 farmers interviewed ranked earthworms as the most destructive pest of terraced rice fields. In a second example the conversion of Amazonian rainforest to pastures has been shown to lead to extinction of the natural earthworm community, which have been replaced in some circumstances by a single exotic species, Pontoscolex corethrurus. This has a negative effect on pasture productivity because the introduced worms compact the soil, whereas the native species improve soil structure (Chauvel et al 1999). Inoculation with species from the forest might reverse this effect, but remains to be tested.As already indicated the decreased genetic diversity of plant cover increases the risk of pest attack. Simplification of the ecosystem and in particular the use of broad-spectrum pesticides also decreases the diversity of natural enemies and increase risks of pest attack (Lawton and Brown 1993). Pesticides also have negative effects on non-target beneficial organisms including pollinators and beneficial soil biota.Land-use change alters the balance of gas emissions and thence influences global climates. There are very large increases in the CO 2 output during clearing from natural vegetation and break down of soil organic matter reserves that are rarely if ever balanced by regrowth. The output of methane may be significantly increased in systems such as paddy rice and intensive cattle production and of nitrous oxides by Nfertilisation. These changes are linked to alterations in soil structure that dominate changes in the activity of a variety of soil organisms (e.g. methanogenic and methanotrophic bacteria) but we are not aware of any documented case where such effects are linked to the absence of functional groups or to biodiversity change per se.There are a few general conclusions that may be drawn from this brief review of the impacts of agricultural intensification on the relationship between biodiversity and ecosystem services. First that whilst there are a number of clear examples where changes in diversity have threatened the provision of ecosystem services, especially relating to the regulation of pests and diseases, there are also others where the changes in biodiversity seem to be functionally neutral, at least within relatively stable environmental conditions. Second there may be some functional groups, particularly micro-organisms such as the decomposers, where the degree of functional redundancy is such that the resilience of the function is very high. These two observations may be generalised by stating that there are no rules to be derived for agricultural systems concerning the importance of biodiversity with respect to the maintenance of ecosystem services that apply across all functional groups and environmental circumstances. Both the concept of 'diversity' and that of 'ecosystem function' are too broad to make generalizations at this level testable. There is a need and potential however to investigate the issues of thresholds of diversity-function relationship within specific functional groups and under circumstances of change in stress and/or disturbance.Finally we should re-emphasise the importance of the hierarchical control exerted by the plants over the other functional groups (Figure 4,Appendix). This is a particularly important feature when determining management options, not only at the field and farm scale but also at that of the landscape. The plant, decomposer and herbivore subsystems of the biological community interact in a variety of ways but the productivity, mass, chemical diversity (resource quality) and physical complexity of the plant component exerts the strongest influence and is the single most important determinant of both the diversity and the functional efficiency of the other two subsystems. Wardle et (1999a and b) and Yeates et al 1999 showed for example that arthropod and microbial communities were not adversely affected by agricultural intensification provided the type of management (eg. mulching) provided for increases in the quantity and quality of the organic inputs. The maintenance of total system diversity and of the major part of the ecosystem services is thus predominantly determined by the nature of the plant community. This is also of course the main point at which humans intervene in the agroecosystem -to decide the species richness, the genetic variability and the organisation in space and time of the planned biota in the vegetation subsystem.A substantial research investment has been made into agricultural systems that fall short of the full extent of genetic homogenisation and petro-chemical substitution. Examples are agroforestry and other intercrops, rotations, mulch-based, minimum tillage and integrated livestock-arable systems. All these systems are characterised by maintenance of diversity of plant functional groups above the level of monocropping. The scientific justification for such approaches has generally been made on grounds of greater functional sustainability and the wider spread of risk associated with more diverse products as well as on the recognition that it is line with the management choices of the majority of the rural poor in the tropics. For farmers labour saving and low investment and risk may be the preferred attributes of these systems.The simplicity of monocultures at field level is only possible as long as farms are part of a germplasm delivery system with rapid access to externalised gene banks and have access to risk buffering mechanisms such as insurance schemes or agricultural subsidies. Large parts of tropical agriculture still operate in a range where such 'externalized' risk management options do not exist and where thus a choice for monocultures carries unaffordable risks. At the farm level ecosystem resilience can be extended beyond resources maintained on farm or in the accessible neighbourhood by being part of a larger agricultural production and germplasm delivery system Ewel (1986) and Moreno and Hart (1979) are among those who have advocated using plant functional groups as a basis for the (plot level) design of multi-plant agroecosystems. These designs also rely, explicitly or implicitly, on the impact that the effect of increasing the diversity of the vegetation system will have in enhancing the associated biodiversity both above-and below-ground and thence the probability of maintaining ecosystem services over a wider range of stress and disturbance. The evidence comparing such systems is almost entirely however based on assessments of yield, Vandermeer et al (1998) reviewed the literature on inter-cropping of all types and concluded that yield gains in comparison with mono-crops depends on the specific complementarities in resource use and seasonal development of the components. As risks for the farmer depend on farm level diversity of potentially productive resources rather than on plot-level diversity, the focus of much agroecological research may have been too narrow.Another key aspect that needs to be changed is the continuing separation of different aspects of management interventions on the base of disciplinary experience, such as soil or nutrient management from pest management. Interventions to ameliorate the impacts on any one of the different ecosystem services (as well as on productivity) are likely to influence others. Practices targeted at productivity but well documented in terms of their supportive, ameliorative or regenerative effect on other ecosystem services should be a top priority.Almost all the evidence that exists for the relationship between diversity and function is for the plot (and often the micro-plot or laboratory chamber) scale. But in order to provide policy makers with appropriate advice on the functional value of diversity it is necessary to consider the ways in which the three factors we have been considering -biodiversity, agricultural productivity and profitability, and ecosystem services -intersect at the landscape scale. Whilst the inter-relationships that we have described at the plot (patch) scale may help in understanding what happens at the landscape scale there is also the possibility that the rules change as one shifts across the scales. The productivity of any land-use system can be expressed on an area basis and the aggregate productivity across a landscape on the basis of the fractions occupied by different land uses. Biodiversity however has more complex scaling relationships and cannot simply be aggregated in this way. Nor can many of the functions that have been discussed here.Much of the diversity in a landscape may exist at scales beyond the farm (between farm variability being larger than within-farm diversity), and the dynamics of diversity thus depend on the degree to which different farms remain (or become more) different. As agricultural research and extension have been based on the economies of scale that are perceived as attainable by homogenisation of farms with similar demands for inputs and services and similar outputs for markets, the trend in agricultural intensification has often resulted in the reduction of inter-farm diversity. This process is generally supported by policy interventions which tend to promote homogeneity in farmer goals, practice and behaviour, at least of over the short term. The agents of change in biodiversity beyond farm level are essentially different from those on farm.In Figure 2 we hypothesise that the relationship between species richness and specific ecosystem services at the landscape scale may follow a relationship analogous with that of the Vitousek-Hooper model -together of course with all the attendant qualifications. That is to say that ecosystem services at the landscape scale are optimised by a diversity of land-uses, but the number that are required for optimisation is relatively small. If the hypothesis is correct then it would suggest that the presence of a relatively small number of different land-use types should be sufficient to satisfy the functional needs of the majority of ecosystem services. This generality needs however to be detailed for any given landscape into specifics with respect to not only the types but also their sizes, shapes, patterns on the landscape and practices of management.It can be further hypothesised that at the higher scales of landscape and region the frequency and intensity of disturbance and stress (both natural and anthropogenic) is greater than those at the plot or farm scale and increasingly beyond the control of the land users. Prevention of decreases in the stability of agroecosystems and management of restoration become more difficult and costly and eventually become impossible from both biological and economic perspectives because connectivity is too high and disturbances too large. . The ecosystem services that enhance the resilience and adaptation of systems, such as biodiversity, thus become more and more important a feature of sustainable management as the scale of operation widens.Figure 3 hypothesises a number of relationships implied in the above discussion. We have argued that at the plot and farm scales individual land managers and farmers manage biodiversity largely through simplification (i.e., by decreasing connectivity and maintaining agroecosystems at a stage of early succession) and substitution. Decreases in connectivity may, under specific conditions reach a threshold level of irreversibility, in which case the agroecosystem loses its resilience. However, the individual land user can in most cases manage and control agroecosystem disturbances and stresses, such as pest outbreaks or sudden changes in relative prices, by making adjustments in the management of resources (land, water, germplasm, knowledge, labour, capital) at the farm scale. This is pictured as the shift from Curve 1 to Curve 2 in Figure 3, which hypothesises some of the scalar implications of the diversityfunction relationship. We know, as shown for small-scale farms in Kenya by Osgood (1998), that many farmers do value genetic and species diversity on their farms, as they are aware that it minimises economic risk by enhancing on-farm diversification of plant and animal production. The history of agriculture provides many examples of how even extreme reductions in biodiversity can be managed, through periods of disturbance, by individual land users by substitution (e.g. chemicals, labour). Therefore, even though biodiversity has important ecological functions at the farm scale, it is nevertheless possible to decrease biodiversity levels very substantially at that scale while maintaining the productivity and resilience of agroecosystems. We postulate however that at higher scales the control and management of disturbances and stresses becomes more and more problematic and costly and the resilience function of biodiversity thus becomes an increasingly important issue in management.We have already emphasised the over-arching influence of the plant cover and diversity on the associated functional diversity and thence on the properties of resilience. The simplest rule for managing landscapes is thus to say that if the vegetation is diverse then the associated diversity and functions will be taken care of. The immediate implication of this is that monotypic landscapes -vast areas of the same crop or livestock system -are likely to be the most vulnerable to the same dangers to ecosystem services pictured earlier for the farm or plot scale. Examples of these effects are the pollution of ground water by nitrates and pesticides in large-scale chemical-based agriculture and the difficulty of controlling epidemics in genetically homogeneous stands of vast area. These however seem simply to be the same issues as those at the plot scale only writ larger. The mechanisms for correction are also the same -diversifying the type of land-use system in space and time.The majority of agricultural landscapes in the tropics, in contrast with most of the northern temperate zones, are indeed mosaics of different land uses. The most sensitive of the ecosystem services at the plot scale is probably the biological pest control system. The management opportunities for this increase with widening scale as greater opportunity for diversity in both genetic signals and physical structure of the vegetation permit a wider diversity and larger reservoir of control organisms. Many of the endangered invertebrates and microorganisms of the soil community are mobile, or may be carried by vectors, and can thus recolonise degraded areas from within mosaics that provide suitable reservoirs. Others (e.g. earthworms) are less so however and re-inoculations may be necessary. In each of these cases the size, pattern of arrangement and rotation in time of land-uses on the landscape will have significant effect on the efficiency of ecosystem service provision. Management at the landscape scale offers greater opportunity than at the plot and farm for varying land-use over time. Izac and Swift (1994) argued that sustainable land management could most easily be achieved at this scale by means of balance between aggrading and degrading areas i.e. between patches of high exploitation and those of fallow or rest. Soil organic matter change is a specific and far-reaching example. In areas of intensive production and harvest the soil carbon content may decrease but under fallow or tree-based production it can be re-built. The balance between these two options affect nutrient cycling, soil structure, water regimes and the emission of greenhouse gases. The policy requirements for such integrated management of landscape mosaics are however very different to the production-related approaches that currently prevail in favour of landscape homgenisation.The third hypothesis of Vandermeer et al (1998) predicts that a higher diversity of species will be required to provide a buffer against stress and disturbance at the landscape scale than will be the case for any single patch within it (i.e. gamma diversity will be higher than the sum of alpha diversity). This is pictured in Figure 3 by the difference between curves 1 and 3. Humans can intervene relatively easily (although not necessarily cost-effectively) at the plot scale to substitute for diversity loss -as represented by the difference between curves 1 and 2. At the landscape scale however intervention by humans, including these substitutive actions, will tend to widen the range of stress and increase the frequency of disturbance and further extend the diversity-function relationship (Curve 4 in Figure 3). Substitutive management for purposes of restoring ecosystem services (analogous to the Curve 1 to 2 relationship in Figure 3) is likely to be prohibitively expensive at this scale and may suffer from a 'free rider' problem where it is difficult to get all beneficiaries to share the costs. We contend therefore that the implication of this hypothesis is of very high risk associated with ignoring landscape scale management and focussing only on policies that promote plot scale interventions. Plot scale activities are more likely to exacerbate landscape scale problems than repair them. On the other hand landscape scale interventions offer great opportunity for improvements at the plot scale by increasing overall integration and resilience. There is thus more functional justification for arguing in favour of maintaining or enhancing biological diversity at the landscape scale than there is at the scale of the plot.This model is of course simplistic and does not provide any guide to other features such as the size, shape and position (pattern) of patches on the landscape or on the temporal relationships between them. The hierarchical relationship between ecosystem services should assist in developing rules for these aspects. The regulation of erosion and water flows operates at a higher level in the hierarchy of controls than do aspects of nutrient cycling, soil structure and gas emissions or pest controls. Van Noordwijk et al (this volume) discuss these higher-level aspects of landscape management under the title of 'watershed services'. The lower level services such as nutrient cycles and biological control activities may then be built in through focus on aspects such as the degree of connection between the patches and the location, direction and intensity of the flows between them. It may be useful to classify land-use types into 'functional groups' in a manner analogous with that for species in order to develop more meaningful relationships between diversity and function at the landscape scale.The changes associated with agricultural intensification, including the attendant processes of diversity reduction and substitution of function, are made in response to food need, market opportunity, and perceptions of increased management efficiency associated with mechanisation. These factors remain a dominant reality within market-orientated agriculture where a small number of specific products have high value and specialisation thus becomes a desirable target. Van Noordwijk and Ong (1999) discussed the paradox that urban consumers have access to an increasingly diverse array of food resources that are produced on specialized farms of greatly reduced internal diversity. Observed changes in diversity at a one scale may thus not represent changes at higher systems levels. The risks to agroecosystem services of simplifying ecosystems and substituting biodiversity by labour and chemicals (e.g., in pest control) are those of losing some keystone functions including the ability of an agroecosystem to adapt to change without further substitutive interventions. The evidence, as briefly described above, that ecosystem services might be significantly impaired in agroecosystems as intensification increases is substantial although the role of biodiversity is far from clearly understood. The farmer may not perceive these effects to be serious if the economic environment enables continuing profit based on subsidies related to the substitution process. This has been the basis of agricultural development in Europe and North America for many decades. It thus appears that to attain the essential goal of profitability, even without petro-chemical substitution, agroecosystem diversity is likely to be kept low and that associated with this low diversity there is a risk of crossing threshold levels for the maintenance of ecosystem services the restoration of which is likely to be extremely costly, let alone feasible. Decisions about the management of agroecosystems in market economies do not normally take into consideration the costs of interfering with ecosystem services, including those in which biodiversity plays a strong influence. But when agroecosystems are driven across thresholds from a desired to an undesirable state, the costs to society of being in this new undesirable state, or of restoration of a more desirable one if it is feasible, can be extremely high. Therein lies the risk of simplifying ecosystems. Holling (1986) provided a seminal analysis of the consequences of a number of such irreversibilities. Policies for sustainable agriculture, i.e. to promote integrative practices that focus on the conservation of resources (including genetic diversity) as well as productivity, have proved elusive. If the policy needs are extended to include the management of biodiversity at the landscape scale in order to protect and enhance a wide range of ecosystem services, the problem becomes more acute. There are two particular reasons why the problem is exacerbated at higher scales. First, population pressure and globalisation of trade and the concomitant land use changes (expansion of cities into agricultural lands and of agriculture into marginal areas) result in increased frequency and intensity of disturbances and stresses by comparison with those at the farm scale. The capacity to correct these effects also diminishes because the sensitivity of the systems increases in concert with their connectivity as one moves up the hierarchy of scales (Holling, 1986).Second, the higher the scale under consideration, the more difficult it is for the increased numbers of individual land users to develop an effective management strategy for agroecosystem disturbances, that takes ecological interactions and connectivity into consideration. Even at the scale of small watersheds, it is not often the case that land users have been successful in developing collective and effective means of control and management of disturbances. Furthermore, even if these land users have full knowledge of the relevant level of connectivity necessary to ensure resilience at the watershed scale, different sectors of society place differing levels of importance on ecosystem services and diversity. Farmers in tropical countries are unlikely to place as high a value on these functions of landscape diversity as does the community at large or the national society. They are furthermore highly unlikely to value the serependic (i.e. future) value of diversity, which is much more likely to be valued by national and global communities.In economic terms, farmers value some of the on-farm benefits of diversity and very few of the off-farm benefits, for the usual reasons that costs and benefits outside of the managers' domain (i.e. externalities) are generally not taken into account by individual decision-makers. The argument is however not simply about off-farm effects of biodiversity being ignored. Farmer knowledge varies greatly. There may be for many a number of on-farm ecosystem services that farmers may be unaware of (e.g., the role of micro-organisms), and thus cannot value, as well as services they may be aware of but will not consider important (e.g., reduction of greenhouse gas emissions). The same services may be valued by other groups in society, with a different perspective and set of interests. What is a beneficial service for one group may also be a cost for another (e.g. the perception of earthworms as 'pests' for paddy rice farmers, the trade-off between carbon sequestration and SOM mining). For these reasons, management of ecosystem services, and of biodiversity at the landscape scale, as well as management of disturbances in agroecosystems in land use mosaics, is unlikely to be optimal, from either an ecological or an economic perspective, in the absence of specific policy or institutional interventions. Lack of knowledge of threshold levels in connectivity at different scales, different perspectives on the value of biodiversity, externalities and difficulties in large groups of land users coming together in developing effective means of controlling disturbances at the landscape scale thus result in biodiversity being managed by individual farmers in a sub-optimal manner.We therefore conclude, on the basis of the relationships we have hypothesised earlier, that it will prove very costly to manage ecosystem services at the watershed, landscape and higher scales unless the functional value of biodiversity for productivity at the plot and farm scale and its interaction with 'externalities' beyond are perceived and valued. Furthermore, unless in particular the role of biodiversity in enhancing resilience is understood and factored into effective policy or institutional interventions, ecosystem diversity is unlikely to be maintained at the landscape scale without deliberate policy interventions at national and sub-national levels which take into account the real value of maintaining ecosystem services, given the externalities they generate and given their contribution to resilience. The biggest challenge is in the realization that most of diversity as well as much of its positive role in resilience probably exists beyond the farm scale, and that thus diversity of management decisions by farmers rather than any specific management system is key to its maintenance in the landscape. These policy implications and the need for diversity enhancing communal action remain largely unexplored territory.There are two final comments that can be made to close this discussion. First that the absence of clear evidence should not be taken as evidence for the absence of effects and thus as a reason for doing nothing. Some economists have proposed that, in view of our relatively poor understanding of the exact roles of biodiversity in ecosystems on the one hand and of the potentially devastating effects of biodiversity loss on the other hand, a precautionary principle should be used in managing diversity. This principle acknowledges that while we may not be able to justify what some see as redundant species, there may be an extinction threshold that would result in an unacceptable level of ecosystem failure. Consequently, extreme care and precaution must be taken, and it is preferable to err on the conservative side (Perrings, 1991). The precautionary principle introduces an important concept, namely that of the risk of managing agroecosystems in such a way that threshold levels of biodiversity loss in relation to ecosystem services are ignored. The 'risk premium' that the precautionary principle suggests is hard to quantify as yet. Second that even if the evidence that high levels of biodiversity are not important for maintenance of ecosystem functions or services holds, that does not contradict the valuation of biodiversity for other reasons.In the above discussion we have quoted or proposed a range of hypotheses concering the relationships between biological diversity and ecosystem functions, and their implications for the management of agriultural landscapes. The general relationships that have been proposed may have to be replaced by more specific hypotheses of the relation between components of overall biodiversity and specific environmental functions, bounded in space ant time. Sweeping generalizations from experiments that are necessarily restricted in space and time, and for example do not include major parts of the diversitygenerating processes (including 'lateral flows' of dispersal and migration for re-establishment), are unlikely to be helpful in guiding the development of agro-ecosystems that have to provide for short, medium and long term service functions. Future investigations should utilise co-evolved communities, be structured to investigate the distinct roles of clearly defined functional groups, separate the effects of between-and within-group diversity and be conducted over a range of stress and disturbance. This might include: testing the basic functional-biodiversity rule by experimentally determining the minimal level of diversity between and within functional groups that is necessary to maintain productivity, integrity and perpetuation of ecosystems; characterising the functional groups of organisms necessary to maintain specific ecosystem services; determining the ecosystem function and service effects that ensue from elimination or substitution of key functional groups, including particular investigation of controls over below-ground diversity and function exerted by particular plant functional groups and other keystone organisms; and determining (and developing indicators for) the biodiversity thresholds for different ecosystem services. An interesting extension of the latter study might be to investigate whether similar thresholds exist for the intrinsic, utilitarian and serependic values of biodiversity.Society as a whole has an interest in ecosystem services that are manifested substantially at scales above that of the field plot or farm. At the scale of the watershed or landscapes there is, in comparison with any single patch, a greater range of environmental stress and higher frequency of disturbance, including of extreme events. The maintenance of ecosystem services at these scales thus requires either a higher diversity of species within functional groups or a greater investment in substitutive management to maintain ecosystem services. These increments in diversity and/or investment are unlikely to be simply additive in view of the significant shifts in complexity that occur with shifts across scale. Optimal maintenance of ecosystem services at the landscape scale may be most readily achieved by a mosaic of a relatively few land-use types. This model is however likely to be overly simple because of: (a) differences in functional impact of different land-use types; and (b) the importance of organisation at the landscape scale in terms of the size, shape and location pattern of the constituent land-uses.In developing appropriate land-use scenarios landscapes should be compared with respect to the aggregate values of their component land-uses for intrinsic, utilitarian and functional (ecosystem service) values of biodiversity. This would be assisted by establishing a typology of land-uses in terms of their efficiency in maintaining ecosystem service and in the trade-offs between this and profitability. The results of the ASB project provide a model for this approach with respect to the interactions between carbon sequestration potential and profitability. The relative costs and benefits of segregating the intrinsic, utilitarian and functional uses of biodiversity between different land-use or landscape units compared with integrating them within such units is another parameter that should be of significant value for policy development.This review confirms two unsurprising but crucial elements for policy development: first that whilst a number of important analogies can be drawn across scales with respect to the management of the relationships between biodiversity and ecosystem services, there are also emergent properties that necessitate different approaches; second that the value placed on the relationship between biodiversity and function (ecosystem services) by individual land-users is markedly different than those perceived by the community at different levels of society. We have indicated a number of biological and socio-economic issues that need to be clarified in order to provide more explicit advice to policy makers. No single optimal value can be placed on the biodiversity within a landscape. Land-use decisions are likely to be optimised if decision makers can be provided with scenarios showing how various land-use combinations result in different levels of diversity and the efficiency of different ecosystem services. In so-doing it will be important to include aspects of temporal change as well as pattern on the landscape as both these factors influence the resilience of the landscapes which should be regarded as a factor of over-riding importance. These scenarios can then be used to identify policy interventions and institutional arrangements necessary to achieve the desired objective, whether it is one dominated by agricultural productivity targets or the maintenance of ecosystem services or the conservation of biodiversity, or a combination of all three. Notes to Table 1.In some ecosystems photosynthetic micro-organisms may constitute as significant group eg. rice ecosystems). Here we deal only with plants.Plants. There is a long history of classification of plants into functional groups. The groupings have been based on a variety of reproductive, architectural and physiological criteria. For the purposes of this paper the efficiency of resource capture is suggested as the main criterion. This will be determined by features of both architecture (eg. position and shape of the canopy and depth and pattern of the rooting system) and physiological efficiency. A very simple classification could for instance distinguish the roles trees, shrubs, vines and cover plants etc. and then subdivisions within each of these groups. Much more detailed consideration of these aspects is given by Smith et al (1997).Primary Regulation (Note 1). These are a set of functional groups which have a significant regulatory effect on primary production and therefore influence the goods and services provided by the plants.Herbivores: A great variety of organisms feed directly on primary producers. Vertebrate grazers and browsers are readily distinguished from invertebrate pests although their impacts on the plants may have similar functional significance at the ecosystem level. Each of these major groups are sub-divisible in terms of, for instance, feeding habits. The balance between different types of browser for instance can influence the structure of the canopy.Parasites: Microbial infections of plants may limit primary production in analogous manner to herbivory. Parasitic associations can also influence the growth pattern of the plants and thence their architecture and physiological efficency.Micro-symbionts: There is a wide range of microbial infections that are beneficial rather than destructive of which the most familiar are di-nitrogen fixing bacteria and mycorrhizal fungi.Service Provision.The functional groups within this category also strongly influence primary production but not in the directly destructive or stimulatory way of the primary regulators. They also provide a set of ecosystem services distinct to those deriving mainly from the primary producers.Decomposers: This is group of great diversity which can be sub-divided taxonomically (bacteria, fungi, invertebrates etc) and in relation to size both of which correlate somewhat with functional roles in the breakdown (eg. detritivorous invertebrates) and mineralisation (fungi and bacteria) of organic materials of plant or animal origin (Swift et al 1979, Lavelle andSpain 2001).Ecosystem Engineers: These are organisms that change the structure of soil by burrowing, transport of soil particles and formation of aggregate structures. The term is often confined to the macrofauna such as earthworms and termites but fungi and bacteria also play a key role in the binding of soil aggregates. Many of these organisms also contribute to the processes of decomposition.A considerable proportion of soil degradation induced by human-related activities is a result of deforestation, overgrazing and improper agricultural practices. Eighty five percent (85%) of agricultural land is estimated to be degraded to some extent (Oldeman and van Lynden, 1997). The mounting evidence of land degradation induced by agriculture is resulting in a gradual shift from a high input agriculture paradigm, based on overcoming soil constraints to fit plant requirements by amending soils with fertilizers, lime, biocides and tillage, to a paradigm with more reliance on biological processes (Sanchez, 1994). This paradigm invokes a more ecological approach based on the adaptation of germplasm to adverse conditions, the enhancement of biological activity of the soil and the optimization of nutrient cycling to minimize external inputs and maximize the efficiency of their use. This new paradigm focuses on the need to improve agricultural production in more benign ways compared with traditional agricultural improvement that is based on high inputs with subsequent detrimental environmental impacts that result in soil degradation. Nevertheless, while this paradigm shift is a good sign its beneficial impact, in terms of improved soil management options for healthier landscapes, will be limited if there is little adoption by local land managers.The limited adoption of new technology and new cropping systems has been often attributed to local inertia rather than the failure to take into account the local experience and needs (Warren, 1991). According to Walker et al. (1995), increased application of indigenous knowledge to rural research and development can be attributed to the need to improve the targeting of research to address client needs and thus increase adoption of technological recommendations derived from research. The complementary role that indigenous knowledge plays to scientific knowledge in agriculture has been increasingly acknowledged (Sandor and Furbee, 1996). Experimental research is an important way to improve the information upon which farmers make decisions. It is questionable, however, if relying on experimental scientific methodology alone is the most efficient way to fill gaps in current understanding about the sustainable management of agroecosystems. There has been limited success of imported concepts and scientific interpretation of tropical soils in bringing desired changes in tropical agriculture. This has led an increasing recognition that local soil knowledge can offer many insights about managing tropical soils sustainably (Hecht, 1990).Local knowledge related to agriculture can be defined as the indigenous skills, knowledge and technology accumulated by local people derived from their direct interaction with the environment (Altieri, 1990).Transfer of information from generation to generation undergoes successive refinement leading to a system of understanding of natural resources and relevant ecological processes (Pawluk et al., 1992). Nevertheless, although benefits of local knowledge include high local relevance and potential sensitivity to complex environmental interactions, without scientific input local definitions can sometimes be inaccurate and unable to cope with environmental change. It is thus argued that research efforts should further explore a suitable balance between scientific precision and local relevance resulting in an improved knowledge base as indicated by Barrios and Trejo (2001). Furthermore, this approach would overcome the limitations of site specificity and the empirical nature of local knowledge and would allow knowledge extrapolation through space and time as suggested by Cook et al. (1998).A common language is required to link local and technical knowledge about soils and their management so that acceptable, cost-effective strategies for improved soil management can be developed. For this purpose a methodological guide has been developed and used in Latin America and the Caribbean (Trejo et al., 1999) and Africa (Barrios et al., 2001) in order to help stakeholders identify and classify local indicators of soil quality (ISQ) related to permanent and modifiable soil properties as this is the first step in the development of local soil quality monitoring systems (Fig. 1).Selecting a suitable set of ISQ, and developing its use as a monitoring system (Soil Quality Monitoring System, SQMS), can be captured in the following figure (modified from Beare et al., 1997):Suitable ISQ are identified from the local and technical knowledge base and critical levels defined. This phase is followed by the definition of guidelines to establish a Soil Quality Monitoring System (SQMS) along with interpretation information as well as reaching an agreement about the suitable ISQ for the relevant conditions. User feedback is very important at this stage as it will provide the grounds for acceptance of the SQMS for soil quality diagnosis and monitoring. Once the SQMS is fully accepted by users it becomes part of the Decision Support System for Natural Resource Management This methodological guide is mainly focused on the first phase of this process; i.e.: identifying soil quality indicators that can be used by farmers, extension officers, NGO's, technicians, researchers and educators. The ISQ will help in identifying the main soil biophysical limitations of the agricultural system under study. The most sensitive and robust ISQs selected for the soil constraints identified can then be incorporated into a Soil Quality Monitoring System (SQMS), and should include basic parameters such as bulk density, pH, effective rooting depth, water content, soil temperature, total C and electrical conductivity (Doran and Parkin, 1994). Since our objective is to develop a SQMS for the land users, local indicators of soil quality must be included in the monitoring system. The mix of native and scientific parameters varies according to the monitoring objectives; e.g.: if they are farmers, extension agents or policies makers. It is likely that integrative ISQ might be more useful to land users, than a measurement, for example, soil available P, since many indicators used by the farmers are also of the integrative type; for instance, soil color, soil structure, crop yield, presence of specific weed species. Attention should be paid to the inclusion of indicators that can be used while progressively increasing the scale at which results are applied (e.g. from plot to field and farm level, up to watershed, region and nation level). Some examples of such indicators might be crop yield and yield trends, land cover, land use intensity and nutrient balances (Pieri et al., 1995). More recently, Defoer and Budelman (2000) have proposed the use of resource and nutrient flows at farm scale to assess land use sustainability and local variation usually missed in studies at higher levels of aggregation (i.e. region, country). The methodological approach proposed by Trejo et al. (1999) and Barrios et al. (2001) rests on the belief that in order for sustainable management of the soil resource to take place, it has to be a result of improved capacities of the local communities to better understand agroecosystem functioning. Improved capacities by technical officers (extension agents, NGO's, researchers) to understand the importance of local knowledge is also part of the methodology. Therefore, after identifying if there is poor or a lack of adequate communication between the technical officers and the local farm community as a major constraint to capacity building, the methodology proposed deals with ways of jointly generating a common knowledge that is well understood by both interest groups. The structure of the guide is shown in Fig. 2 shows the different sections of the methodological guide for Africa.This methodological guide is made up of six sections: Section 1 provides a general introduction about the management of the soil resource in the African context and the ISQ. Section 2 presents a technical conception of the soil through a Simplified Model of Soil Formation (SMSF) based on Jenny's seminal work (Jenny, 1941;1980) in order to bring participants to a common starting point. order to carry out several ranking exercises for the same information and thus obtain a more representative mean value. All results obtained from each group conducting the ranking exercise are put together in a ranking matrix where rows represent all local indicators identified during brainstorming and the columns represent the ranking assigned by different small groups of farmers.Results to date indicate that biological indicators like native flora and soil macrofauna are important components of local indicators of soil quality. This is not surprising as biological indicators have the potential to capture subtle changes in soil quality because of their integrative nature. They simultaneously reflect changes in the physical, chemical and biological characteristics of the soil. There is considerable scope, therefore, to further explore the use of local knowledge about biological indicators of soil quality and as a tool guiding soil management decisions.Section 4 provides a methodology to construct an effective channel of communication by finding correspondence between TISQ and LISQ which permit a better Extension/NGO officier, NGO -farmer communication. This is carried out in a plennary session exercise of integration where the most important local indicators of soil quality are analyzed in the context of technical knowledge and are classified into indicators of permanent or modifiable soil properties (Table 2). The classification of local indicators into permanent and modifiable factors provides a useful division that helps to focus on those where improved management could have the greatest impact. This strategy is particularly sound when there is considerable need to produce tangible results in a relatively short time in order to maintain farmer interest as well as to develop the credibility and trust needed for wider adoption of alternative soil management practices.Although some local indicators can be rather general like fertility, slope, productivity and age under fallow, other local indicators are more specific. For instance, plant species growing in fallows, soil depth, color, water holding capacity and predominant soil particle sizes provide indicators that can be easily integrated with technical indicators of soil quality.Section 5 is concerned with management principles behind potential strategies to address constraints modifiable in the short (< 2 yrs), medium (2-6 yrs) and long (> 6 yrs) term (Fig. 3).Modifiable constraints are those that can be overcome through management. Examples include low nutrient and water availability, low and high pH, soil compaction and low soil organic matter content. The discrimination between short, medium and long term is necessary to enable ranking of management strategies, which is mainly dictated by resource endowment.Section 6 is devoted to the Soils Fair which is designed to help farmers develop skills to characterize relevant physical, chemical and biological properties of their soils through simple methods that can then be related to their local knowledge about soil management. Here farmers and scientists communicate through a commonly developed language and simple demonstrations on how to measure soil quality in situ to solve local soil management and land degradation problems.The result of this two-way exchange process is that it has a positive impact on the technical knowledge by nurturing it with local perceptions and demands. Positive impacts are also envisioned on the local knowledge base as it provides with a way for this tacit knowledge to be widely understood, assessed and utilized. Besides, local communities will be empowered by the joint ownership of the technical-local soil knowledge base constructed during this process.The two-way improvement of communication channels will likely improve the communication of farmer's perceptions to extension agents and researchers as well as make recommendations by extension agents and NGOs better understood by the farmer community. Better communication opens opportunities for established and/or emerging local organizations to use this methodological approach for consensus building that precede collective actions resulting in the adoption of improved soil management strategies at the landscape scale.This methodological guide aims to empower local communities to better manage their soil resource through better decision making and local monitoring of their environment. It is also designed to steer management towards solutions to the soil constrains identified as well as to monitor the impact of management strategies implemented to address such constraints.The approach summarized in the preceeding sections provides the tools to conduct a technicallocal classification of the soil, based on modifiable and permanent soil properties, which has the flexibility to work in the spatial scale continuum plot/farm/landscape (watershed) while also having the potential to take the stakeholder groups and gender issues dimensions into consideration. This guide then provides a valuable tool to evaluate the impact of the land use change across various spatial scales and social actors.Finally, participants in the training event associated with the guide are encouraged to develop \"action plans\". These action plans show the institutional commitment made by participants to apply the guide and gained insights in their own work plans and environments. To date more than 23 action plans have been initiated in Latin America and Africa. Follow up of these action plans in the coming years will provide a measure of the impact of this participatory approach in better natural resource management through improved soil management strategies.Current estimates of degradation of the soil resource indicate that we cannot afford to adopt a grow-now and-clean-up-later approach to development. Farmers need early warning signals and monitoring tools to help them assess the status of their soils, since by the time degradation is visible Constraints identification Classify modifiable constraints (short, medium and long term)Design soil management strategies for short, medium and long term modifiable constraints NGOs, Extension agents Search for specific locally available options 632 because of unsuitable management, it is either too late of too expensive to revert it. The costs of preventing soil degradation are several times less than costs of remedial actions. More often than not technical solutions to soil degradation abound but are often left on the scientist shelves because they are developed without the participation of the land user or do not build on local knowledge of soil management. Participatory approaches involving group dynamics and consensus building are likely to be key to adoption of improved soil management strategies beyond the farm-plot scale to the landscape scale through the required collective action process. Action plans developed by local actors as a result of consensus building and new insights derived from the training exercise become a vehicle by which profitable and resource conserving land management is locally promoted and widely adopted. Taking adavantage of the complementary nature of local and scientific knowledge is highlighted as an overall strategy for sustainable soil management.The development of this methodological guide has been a good example of 'South -South' cooperation where experiences from Latin America were brought and adapted to the African context, and feedback from Africa has helped further improvement of the Latin American guide.Long-term management of phosphorus, nitrogen, crop residue, soil tillage and crop rotation in the Sahel Since 1986 a long-term soil fertility management was established by ICRISAT Sahelian Center to study the sustainability of pearl millet based cropping systems in relation to management of N, P, and crop residue, rotation of cereal with cowpea and soil tillage. The traditional farmers' practices yields 146 kg/ha of pearl millet grain whereas with application of 13 kg P/ha, 30 kg N/ha and crop residue in pearl millet following cowpea yielded 1866 kg/ha of pearl millet grain. These results clearly indicate the high potential to increase the staple pearl millet yields in the very poor Sahelian soils.The Kabete long-term trial was started by KARI at the National Agricultural Laboratories site, on a humic Nitisol in 1976. The objective of the trial was to find appropriate methods for maintaining and improving the productivity of soil through the use of inorganic N and P fertilizers, farmyard manures and crop residues under maize-bean rotation practices that are common to small-scale farmers. In 2001, samples were collected from key treatments to study P dynamics and to examine the effects of the different treatments on P pools and P availability. The results will be given in the next progress report.Long-term management of manure, crop residues and fertilizers in different cropping systems Since 1993 a factorial experiment was initiated at the research station of ICRISAT Sahelian Center at Sadore, Niger. The first factor was three levels of fertilizers (0, 4.4 kg P + 15 kg N/ha, 13kg P + 45 kg N/ha), the second factor was crop residue applied at (300, 900 and 2700 kg/ha) and the third factor was manure applied at (300, 900 and 2700 kg/ha). The cropping systems are continuous pearl millet, pearl millet in rotation with cowpea and pearl millet in association with cowpea. The analysis of variance data indicate that fertilizer; crop residue and manure application resulted in a highly significant effect of both pearl millet grain and total dry matter yields. Fertilizer alone account for 34% in the total variation of the dry matter whereas manure account for 18%. Although some interactions are significant they account for les than 3% in the total variation.For pearl millet grain, the application fertilizer, manure, crop residue and cropping systems alone account for 66% of the total variation. The farmer's practices yield 236 kg/ha; the application of 13 kg P and 45 kg N/ha yielded 800 kg/ha but when these mineral fertilizers are combined with 2.7 t/ha of manure or crop residue in rotation with cowpea, yield of 1500 kg/ha can be achieved.The N and P fertilizer value of manure and crop residue is 27 and 13 respectively and the N and P equivalency of manure is 113% and 153% for crop residue. The high values of fertilizers equivalency of manure and crop residue over 100% suggest that the organic amendment have beneficial roles other than the addition of plant nutrient such as addition of micronutrients and better water holding capacity. In addition, the release of nutrient with mineralization over time can match more the plant demand and this will result in higher nutrient use efficiency from the organic amendments. It is also well established that the application of organic amendments can reduce the capacity of the soil to fix P and then increase P availability to plant.Although the combined application of organic resources and mineral inputs forms the technical backbone of the Integrated Soil Fertility Management approach, procuring a sufficient amount of organic matter of a desired quality is very often a problem farmers are facing. While high quality organic resources (high %N, low %lignin and polyphenols) are known to behave as fertilizers through fast mineralization of their tissue N, lower quality organic resources are often more abundant on farmers' fields. Examples of such lower quality resources are crop residues or farmyard manure.Sole application of low quality organic resources may lead to N immobilization and reduced crop growth. Consequently, mineral N is required to overcome the demand for N by the microbial decomposer community and to supply N to the crop. While preliminary evidence shows that high quality resources rarely cause immobilization of mineral N, low quality organic resources may lead to immobilization of fertilizer N. Depending on whether this immobilization phase lasts or not, decreased or enhanced crop yields may be the result. In the case of long-term immobilization, residual effects may be more relevant rather than immediate N supply to the crop.In 2002, network experiments were conducted at 7 benchmark locations across 7 countries to investigate the nitrogen and phosphorus contribution of different low quality organic materials that are available for direct use by farmers.Soils in the Sahel are acidic and inherently low in nutrients with ECEC of less than 1 cmol/kg for all the sites except Gaya where the organic carbon is slightly higher and an ECEC of 1.3 cmol/kg. The data on phosphorus sorption isotherm clearly indicated that most of the soils have very low capacity to fix P due to their sandy nature.As manure was used in most of the trials in combination with mineral fertilizer, a systematic chemical characterization of the manure used at the different sites was undertaken. These analysis will be used to determine the fertilizer equivalencies of different manure sources for nitrogen and phosphorus. The nitrogen and phosphorus levels in the manure were very low and varied from 0.47% to 0.71% and the P levels varied from 0.08% to 0.38%.Interaction of N, P and manure. A factorial experiment of manure (0, 2 and 4 t/ha), nitrogen (0, 30 and 60 kg N/ha) and phosphorus (0, 6.5 and 13 kg P/ha) was established in Banizoumbou to assess the fertilizer equivalency of manure for N and P. The data show a very significant effect of N, P and manure on pearl millet yield. Whereas P alone accounted for 60% of the total variation, nitrogen accounted for less than 5% in the total variation indicating that P is the most limiting factors at this site. Manure account for 8% in the total variation.15N dilution technique was used to quantify the biological nitrogen fixation of three cowpea varieties (local, TN5-78 and Dan illa) under different soil fertility conditions. A non-fixing (NF) cowpea variety was used as non-fixing crop. The samples have been sent to the International Atomic Energy Agency in Vienna, Austria for mass spectrophotometer analysis of 15N in order to assess the biological nitrogen fixation.The data clearly indicate the comparative advantage to combine organic and inorganic plant nutrients for the low suffering soils in the Sahel. The use of only organic P sources yield 5000 t/ha of cowpea fodder whereas the application in the organic farm gave 5718 t/ha. Site 2: Maseno, Western Kenya. An integrated nutrient management experiment at maseno was established in the highlands of Western Kenya on a nitisol at an elevation of 1420 m ASL and receiving an annual rainfall of about 1800mm distributed over two growing seasons. Farmyard manure (quality parameters) was used as the low quality organic resource and was integrated with 0, 30, 60 and 90 kg N ha -1 . Since this was a poor season, the overall grain yield and subsequent response to N was poor. However at 0-30 N levels, treatments integrated with organics consistently yielded higher than urea-N. These differences declined beyond 30N. In contrast, these manures appeared to be effective in overcoming P deficiency that is widespread on farms in Western Kenya.The experiment was conducted in collaboration with the Institut d' Economic Rurale (IER), Mali at the research station in Niono. The site was located at Kogoni in the rice-growing region. Low quality manures derived from livestock fed predominantly rice residues were used in combination with urea-N at 0, 30, 60, 90 and 120kg ha-1. The data show rice yield response to N in the presence or absence these manures. Application of 90-120 kg N gave the highest paddy yield (approx 7.5 t ha -1 ) thereby doubling yield over the control. Integration with manure did not significantly increase the rice yields at any N levels; rather there was a slight additive effect of applying the low quality material.Site 4: Farakou Ba, Kou Valley, Burkina Faso In Burkina Faso, trials were conducted at the Kou valley research station in collaboration with the INERA. The low quality organic input was manure (<1.0%N). The test crop was irrigated rice. The manure applied at 1, 2, 3 and 4 tons dry matter per hectare was combined with urea-N at 0, 40, 80 and 120 Kg N ha -1 . The data show rice yield response to urea-N alone or in combination with organic matter at 4 levels. Applications of N alone doubled rice grain yield over the unfertilized control. There was an additive increase when organic matter was integrated with inorganic-N at all manure levels, however this increase was not significant.The experiments at Zaria are conducted in collaboration with Ahmadou Bello University. The site is located adjacent to the Danayamaka village to the North of Zaria within the Guinean zone. Low quality manure that is typical of farmer organic resource input was used. The manure applied at 1, 2, 3 and 4 t dm ha-1 was combined with 0, 30, 60 and 90 kg N ha-1 in a split plot design arrangement where the main plots were treated with N at 4 levels and the sub-plots received manure inputs at 4 levels. The data show the yield obtained with sole applications of urea-N (response curve) or urea-N in combination with organic manure inputs. At this site, additive effects of manure and fertilizer combinations were not significant indicating that these manures contributed little to the N demand for the maize crop. In addition, the data show that these low quality manures can contribute significantly to overcome P deficiency to maize crop.The Soils And Fertilizer Research Institute was the implementing partner for the network experiments in Ghana. The benchmark site was located within the humid forest zone north of Kumasi. The trial was arranged as a randomized block design on a uniform site that was recently cleared. Low quality maize stover and giant panicum grass were tested as possible organic resources that can be combined with mineral fertilizers for soil fertility improvements. However, the giant panicum treatments did not have sufficient replication to warrant being included in this report. In conclusion, the results for this site are not yet available.The site in Togo is located at Davie within the derived savannah zone. Partners from ITRA, Togo implemented the network experiments. For INM1, local organic resources used consisted of rice residues, which were obtained from an adjacent rice scheme. In order to achieve the required weight in dry matter equivalent for the materials added, not all residues could be incorporated and some of it remained as surface mulch for prolonged periods of time during the growing season. The response of maize to the application of rice residues. There was little or no response to N at this site, probably due to moisture stress resulting from drought during the first season Site 8: Kabete, Kenya In addition to the long-term trial that was earlier reported an adjacent experiment was established to investigate the optimum combination of organic and inorganic N sources. Three different materials of differing quality were applied at 60 kg N/ha equivalent. At this N rate, the fertilizer equivalency value of tithonia was 100% while for senna and calliandra it was 43% and 38% respectively. This indicated that tithonia was as good as urea in supplying N to maize crop.Experiments were established at Maseno in Western Kenya, Zaria in Nigeria, Kumasi in Ghana and Davie in TogoThese experiments were to investigate Optimum N and P management in legume-cereal rotations. Although the combined application of organic resources and mineral inputs forms the technical backbone of the Integrated Soil Fertility Management approach, procuring a sufficient amount of organic matter of a desired quality is very often a problem farmers are facing. In-situ production of organic matter is an attractive alternative to technologies harvesting the organic resources from other sites within or outside the farm. Opting for legumes during the organic resource production phase has the potential to enrich the soil with N through biological N 2 fixation.Herbaceous or green manure legumes usually leave substantial amounts of N in the soil although when left to grow to maturity, harvesting the seeds may substantially reduce the net N input into the soil. 'Traditional' grain legume germplasm has a large N harvest index indicating that although a significant part of the N taken up by the legume was certainly fixed from the atmosphere, more N was taken away during grain harvest resulting in a negative net N input. However, dual-purpose germplasm is now available for, e.g., cowpea and soybean, which produces substantial amounts of haulms besides grains and has a relatively low N harvest index. As such, a net N input into the soil can be expected. Besides fixing N, certain legumes are also known to access less available P pools, alter the soil pest spectrum or improve soil biological properties. These benefits are often summarized as non-N benefits. The effect of a legume on a following cereal crop is often expressed as its N equivalent. One needs to take into account that the processes mentioned above might also lead to a better utilization of legume or fertilizer N although the improved yields are not necessarily an improvement of N supply.The current experiments aim at quantifying the contribution of herbaceous and grain legumes to N supply and, where relevant, at quantifying the impact of targeting P to certain phases of the rotation on the overall yield. No data is available for this report yet as we will be able to monitor the rotation effect only during the next cropping season.Single Superphosphate (SSP), Tahoua Phosphate Rock (TPR) and Kodjari Phosphate Rock (PRK) were broadcast (bc) and/or hill placed (HP). For pearl millet grain P use efficiency for broadcasting SSP at 13 kg P/ha was 18 kg/kg but hill placement of SSP at 4 kg P/ha gave a PUE of 83 kg/kg P. Whereas the PUE of TPR broadcast was 16 kg grain/kg P, the value increased to 34 kg/kg P when additional SSP was applied as hill placed at 4 kg P/ha. For cowpea fodder PUE for SSP broadcast was 96 kg/kg P but the hill placement of 4 kg P/ha gave a PUE of 461 kg/kg P. Those data clearly indicate that P placement can drastically increase P use efficiency and the placement of small quantities of water-soluble P fertilizers can also improve the effectiveness of phosphate rock .A complete factorial experiment was carried out with three levels of manure (0, 3, 6t/ha) three level of P (0, 6.5 and 13 kg/P ha) using two methods of application (broadcast and hill placement).The response of millet to P and manure for the two methods of application. For pearl millet grain the hill placement of manure performed better than broadcasting and with no application of P fertilizer, broadcasting 3 t/ha of manure resulted on pearl millet grain field of 700 kg/ha whereas the point placement of the same quantity of manure gave about 1000 kg/ha. Cowpea are showing also the same effect as for pearl millet.A complete factorial experiment of three level of P (0, 13 and 26 kg P/ha), three levels of N (0, 30 and 60 kg N/ha), and three levels of manure (0, 2, 4 t/ha) was carried out. For pearl millet grain, the optimum combination of organic and inorganic soil amendment gave yield of about 2 t/ha whereas the control yield was 450 kg/ha. The P and N fertilizer equivalency of manure range from 291 to 397%.Past research results indicated a very attractive technology consisting of hill placement of small quantities of P fertilizers. With DAP containing 46% P2O5 and a compound NPK fertilizer (15-15-15) containing only 15% P2O5, fields trials were carried out by farmers on 56 plot per treatment to compare the economic advantage of the two sources of P for millet production. As hill placement can result in soil P mining another treatment was added consisting of application of phosphate rock at 13 kg P/ha plus hill placement of 4 kg P/ha as NPK compound fertilizers.The data clearly shows that there was no difference between hill placement of DAP and 15-15-15 indicating that with the low cost per unit of P associated with DAP, this source of fertilizer should be recommended to farmers. The basal application of Tahoua Phosphate rock gave about additional 300 kg/ha of pearl millet grain. The combination of hill placement of water-soluble P fertilizer with phosphate rock seems a very attractive option for the resource poor farmers in this region. The data clearly show that the application of Tahoua PR with hill placement of water soluble P outperformed the other treatments in most instances.Low, medium and high inputs of mineral fertilizers evaluation Farmers' practices were compared to a low input system consistency on increasing crop planting density at recommended level, a medium input where Tahoua Phosphate rock was applied at 13 kg P/ha and SSP hill placed at 4 kg P/ha and high input as recommended by the extension services where SSP is broadcast at 13 kg P/ha with nitrogen applied at 30 kg N/ha as urea.The data indicates that grain yield can be increased three fold with the medium input and higher economic returns can be anticipated with this treatment. The data show how the yield of the technologies evaluated fluctuated as compared to the farmers' practices with the high input systems dominating the other systems in most instances.As for Karabedji, DAP, NPK and SSP were compared and there was any significant effect between the three sources and yield can be increased for more than two fold with this low input technology.","tokenCount":"202088"} \ No newline at end of file diff --git a/data/part_6/0139733124.json b/data/part_6/0139733124.json new file mode 100644 index 0000000000000000000000000000000000000000..4927c9431f56a802c057c6f746e831bb5557d42a --- /dev/null +++ b/data/part_6/0139733124.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"dc941c40c0c119abe224fa6acb8d0dc6","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c5e99784-f806-4f79-be0f-e7df275a1de8/retrieve","id":"1187391601"},"keywords":[],"sieverID":"56381c5b-d422-43e0-baab-31e248c34c55","pagecount":"5","content":"Food Systems Actors engaged in the cocreation of agroecological innovations -Results of Year 2 engagement Simone Staiger-Rivas, Guillermo Orjuela Alliance Bioversity -CIATThe CGIAR initiative on Agroecology is actively engaging with food system actors (FSA) in eight countries (Burkina Faso, India, Kenya, Lao PDR, Peru, Senegal, Tunisia, and Zimbabwe), particularly to codesign, test, and adapt agroecological innovations, both technological and institutional, from food production to consumption. At the core is the necessity to generate scientific evidence that shows how agroecological principles applied in different socio-ecological systems are better able to provide equity, productivity, economic and environmental benefits than alternatives, including the status quo.The engagement takes place in Agroecological Living Landscapes (ALLs) that are formed in selected territories of each country with diverse stakeholders, including farmer associations or communities, researchers from multiple disciplines, extensionists, private companies, international and national nongovernmental organizations as well as local, regional, and national policymakers. The establishment of ALLs does not follow a standard methodology: Each country's context leads to a different agroecological transition pathway(s) and multi stakeholder approaches.The engagement of food system actors is addressed in the monitoring, evaluation, learning and impact assessment (MELIA) component of the initiative. Country teams collect and report data quarterly based on the following definitions: FSA are defined as private sector agents, policymakers, and female and male smallscale farmers, researchers, communities, investors. They engage with the initiative when they participate in meetings and activities that aim at assessing, co-designing and testing agroecological innovations at farm, market and policy levels. The co-creation process consists in FSA working together and having an equal voice in the activities that aim at developing culturally relevant innovations. Agroecological innovations in turn are of technological and institutional nature and concern the broad range of Agroecological principles (HLPE, 2019). This summary analyzes the data from 2022, and data collected from January to October 2023. It includes seven countries, the data from Senegal missing as it is the latest country to join the Initiative. A report covering all eight countries and the entire two-year span will be published early 2024.Technical ReportTo date, a total of 4,398 FSA has been engaged.Most Initiative outcome targets are already achieved or close to be achieved. The proposed target statement intends 225 national and international researchers to collaborate with FSAs -at least 5,500 farmers, 54 policymakers, 25 private-sector companies-in the co-design and testing of context-specific agroecological innovations (Figure 1). The initiative is effectively engaging the private sector and policymakers and will most probably surpass its targets. The gender balance is almost equal with 49.7% female versus 50.27% male FSA engaged, although with variability between countries. Zimbabwe has engaged the highest number of FSA, followed by Tunisia, India, and Kenya. With respect to gender, India, Kenya and Zimbabwe have engaged more female FSA than male.In total, 42% of the FSA engaged are female, 58% are male (Figure 2). Engaging men and women in the development of innovations enables to address the limitations of the adoption of innovations according to gender, as well as to adapt technologies to different users. Farmers have been engaged in all work packages: To no surprise farmers have been massively engaged in the formation of the ALLs and the decision on the agroecological innovations to be tested (WP1), but they have also been part not only on technical solutions but also on the socio-political dimensions beyond the farm level that are needed to positively affect food systems. of agroecological assessments (WP2), the analysis of value chains (WP3), the discussions on suitable policy and institutional arrangements for AE transitions (WP4) and they participated in exercises to understand the behavioral change contexts.Further analysis of FSA typology with WP and country leads will inform strategies to achieve WP outcomes.A closer look at the country data will help to analyze which FSA are or could be critical to make AE transitions a reality. While the Initiative has targets related to the number of FSA engaged, the intensity of engagement (in several WPs, and in several stages of co-creation) will be further analyzed. Key Takeaways Engagement of food system actors has a key element in the effective pursuit of results and the success of the Agroecology initiative. FSA engaged have been a constant pursuit that reflects the motivational environment during the implementation phase and has been manifested through various affective and social processes through the different work packages of the initiative. The initiative has successfully improved engagement among stakeholders at the farmer, producer organization, and policy levels. This aligns with the recommendations from agroecology research, which underscores the importance of fostering links between actors at various levels for the success of agroecology as a science and a practice.The varied contexts of this initiative's implementation demonstrate that agroecology does not have a singular point of entry. In some countries, the focus has been primarily on collaborating with policymakers, whereas in others, it has been on working with producer organizations. Identifying key players within a community also enables the leveraging of its social capital for the expansion of agroecology. ","tokenCount":"830"} \ No newline at end of file diff --git a/data/part_6/0141596176.json b/data/part_6/0141596176.json new file mode 100644 index 0000000000000000000000000000000000000000..3a584cd2ca0ab38e21ae9460d62d2e23a55c4953 --- /dev/null +++ b/data/part_6/0141596176.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"09882e3f6769773a059a7ed7ab8a389f","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/28cf4cf8-de70-4f08-acdd-e201e71d9682/retrieve","id":"938632941"},"keywords":[],"sieverID":"0b97dd12-6f40-4568-851d-df61063cd5fb","pagecount":"1","content":"• Selection of the field with careful study on cropping / disease history. • Avoid maize planting, where neighbor field is with older maize crop with suspected MLN symptomatic plants. 2 Preparation of Field 3 Use of Certified -MLN disease free seeds 4 Use of Clean / disinfected farm tools and equipments 5 Scouting, rouging, and clean crop cultivation 6 Managing insect vectors 7 Rouging and incinerating infected plant parts 8 Crop rotation and Host free period• Use only certified seeds produced in MLN free areas for each growing season • Avoid using grain as seeds, and seeds from previous infected maize plants or fields.• Clean farm equipment/tools using disinfectants before and after use to eliminate MLN virus contamination.• Monitor the field every week for presence of insect vector population. A high insect vector population increases chances of MLN infection. • Maintain a clean farm by removing grasses, weeds and other alternative hosts from fields.• Scout weekly for MLN viral symptoms for early detection and control of insect vectors. Uproot MLN symptomatic plants and destroy them through burning. • Do not feed MLN-infected plants to livestock, such as cattle.• Control of insect vectors can be done using recommended insecticides (once every 1-2 weeks). • Spray insecticides either in the morning or evening but not in hot and windy period. Before roguing plants, spray systemic insecticides.• Practice crop rotation for at least one season by growing noncereal crops preferably legumes (beans, soybean and peas). The CGIAR Plant Health Initiative will continue to focus on mitigating the threat of MLN in sub-Saharan Africa through partnerships with national and regional partners on:1. Monitoring and surveillance of MLN by the NPPOs. 2. Implementing an integrated disease management strategy, including MLN-free commercial seed production and deployment, and promoting awareness of farming communities on MLN and its management. ","tokenCount":"302"} \ No newline at end of file diff --git a/data/part_6/0148347666.json b/data/part_6/0148347666.json new file mode 100644 index 0000000000000000000000000000000000000000..45cbfb0a4fa42e0067ad7c8f3f285840b7a749ae --- /dev/null +++ b/data/part_6/0148347666.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"900d23b4aec712c95b6f0d9210334eb1","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/3a8a2d9d-95c7-45a7-bc1e-0a1bc34f323b/retrieve","id":"1231618023"},"keywords":[],"sieverID":"4b465fa0-8a1a-4fb8-9219-0cf9dcdde4fc","pagecount":"36","content":"Climatic changes linked to global warming are already having a devastating impact on the lives and livelihoods of farmers and rural communities across the ACP regions. Current climate projections suggest this impact will worsen. Reducing agriculture's contribution to greenhouse gas emissions, while also increasing productivity in a sustainable way is the challenge that lies ahead.World religious and political leaders -from Pope Francis to President Barack Obama -are calling for action against climate change to avoid catastrophic consequences, particularly for the poor. How these calls will result in a comprehensive climate deal in December 2015 in Paris remains to be seen. Smallholder farmers in developing countries are likely to be hardest hit by climate change due to agriculture's vulnerability to extreme weather patterns and the low level of resilience of farming to climate variability and change.The good news is that innovative approaches are being developed to meet this challenge. One of these approaches, climatesmart agriculture (CSA), aims to increase farm productivity and incomes in a sustainable manner, enable farmers to adapt and build resilience to climate change and, where possible, reduce greenhouse gas emissions.To make CSA work, farmers and government decision-makers need support. First, they need reliable information to identify proven solutions to climate change and make informed decisions. Secondly, cooperation among institutions is essential to develop and disseminate best practices and promote conducive policies for CSA.CTA has been playing an active role in a number of partnerships and alliances to support farmers and policymakers. It is an active member of the Global Alliance for CSA, which seeks to enable 500 million farmers to practise CSA by 2030. CTA also facilitates the identification of solutions and sharing of knowledge among farmers and other key players by helping them to document proven practices, tools or policies that promote resilience to climate change. For example, through the use of participatory 3-D mapping, CTA and its partners have assisted local communities to identify their vulnerabilities to climate change and develop solutions to address them.As the climate changes, agriculture needs to transform so that it becomes more profitable, sustainable and resilient. The smallholder farmers and producers who face the hard realities of the impacts of climate change on their livelihoods want practical solutions that work for them and their families. The agriculture and climate communities should not fail them.hilst the climate has changed over the millennia mankind has been on the earth, there is increasing evidence that the world is experiencing climate change on a scale never previously known in human history. Whilst not all climate change impacts are projected to be negative and there will be some regions where beneficial impacts are projected -for many regions, global warming will bring about a decrease in annual and seasonal rainfall, more erratic weather patterns and more intense and frequent extreme weather events, including heatwaves, droughts, storms and floods. Lower relative humidity due to decreased rainfall will favour increased insect vectors and viruses, whilst higher relative humidity due to increased rainfall will favour increased bacterial and fungal infection. Human migration due to environmental pressures is also likely to accelerate.In the oceans, increasing temperatures, acidification and changing currents will impact on fisheries (see p 8, Changing the climate of fisheries in the Pacific); rising sea levels are already leading to salt water intrusion and loss of available land in small islands (see p 23, Responding to climate change), and many other coastal areas. Climate change will also impact on livestock production through heat-induced stress and reduced water availability, as well as changes in availability, quality and prices of fodder (see p 10, Building resilience in Caribbean livestock farming).To meet the needs of the world's expanding population, which is projected to be 9 billion by 2050, farmers will have to produce more; this task will be made all the more difficult by climate change. Even a 2°C rise -which is the median projection (see p 7 -Table 1) will lead to dramatic changes in agricultural productivity and water availability. The added challenge is to produce more but in ways that will protect the environment, especially soil and water, whilst minimising agriculture's contribution to climate change. Indeed, whilst many still regard agriculture as a 'victim' of climate change, the impact of agriculture itself on global warming cannot be overlooked.Around 14% of human-generated greenhouse gases are estimated to come directly from agriculture; for example, almost half of all methane and nearly 60% of nitrous oxide emissions are generated by agricultural activities, including livestock production, and fertiliser and pesticide applications. A further 18% of greenhouse gases come from land use changes (e.g. clearance of forests for crops and pasture), soil erosion or machineintensive farming methods, which also contribute to increased carbon dioxide concentrations in the atmosphere. Reducing agriculture's carbon footprint is therefore an important consideration to limiting climate change. And to help ensure food security, farmers across the globe will probably need to switch to more climate-hardy crop varieties or even change the crops they grow, as well as their farming practices.Action that helps offset the effects of climate change. For example, the construction of barriers to protect against rising sea levels, or conversion to crops or crop varieties capable of surviving high temperatures and drought.Climate change caused by greenhouse gas emissions resulting from human activity as opposed to natural processes.Scenario used for projections of future emissions assuming no action, or no new action, is taken to mitigate the problem.Occurs naturally and is also a by-product of human activities such as burning fossil fuels. It is the principal greenhouse gas produced by human activity.The process of converting CO 2 gas into a solid form, for example when trees convert CO 2 into biomass (wood, leaves, roots etc.).The steady rise in global average surface atmospheric temperatures, which experts believe is linked to greenhouse gas emissions resulting from human activity.Natural and industrial gases that trap heat from the Earth, warming the surface. The Kyoto Protocol restricts emissions of six GHGs: natural (carbon dioxide, nitrous oxide, and methane) and industrial (perfluorocarbons, hydrofluorocarbons, and sulphur hexafluoride).The insulating effect of certain gases in the atmosphere, which allow solar radiation to warm the Earth and then prevent some of the heat from escaping. The second most important GHG. Sources include the natural world (wetlands, termites, wildfires) and human activity (crop production, livestock rearing, waste dumps, leaks from coal mining).Action that will reduce human contributions to climate change, including action to reduce GHG emissions and levels of GHGs in the atmosphere.An important GHG. About 80% of the global emissions of nitrous oxide come from the agricultural sector, largely from soils which have been ameliorated with organic and inorganic nitrogen fertiliser.When CO 2 dissolves in seawater, carbonic acid is formed. Carbon emissions in the industrial era have already lowered the pH of seawater by 0.1. Ocean acidification decreases the ability of marine organisms to build their shells and skeletal structures and kills off coral reefs.Reducing Emissions from Deforestation and forest Degradation, a concept that provides developing countries with a financial incentive to preserve forests.Is a threshold for change, which, when reached, results in a process that is difficult to reverse. Scientists say it is urgent that policymakers halve global CO 2 emissions over the next 50 years or risk triggering climatic changes that could be irreversible.The United Nations Framework Convention on Climate Change aims to prevent \"dangerous\" human interference with the climate system. It entered into force on 21 March 1994 and has been ratified by 192 countries.OCTOBER 2015 | SPORE SPECIAL ISSUE |However, climate change does not occur in the same way and with the same impact across ACP regions and the adaptation and mitigation responses required will also be different. For example, about 90% of the sub-Saharan African population depends on rainfed agriculture for food production and, according to the latest IPCC report, could result in decreased crop yields of 18% for southern Africa to 22% across sub-Saharan Africa. At the same time, Africa's population continues to grow; annual growth is estimated at 2.4% and the population is predicted to double to 1.8 billion by 2050. According to the FAO, to feed the projected population, crop production will need to increase by 260% by 2050, yet crop models, used by the International Food Policy Research Institute indicate that by 2050, if current low input crop management practices were maintained, average rice, wheat, and maize yields in sub-Saharan Africa will decline by up to 14%, 22%, and 5%, respectively, as a result of climate change (see p 9, Cocoa trees love the shade).In the Pacific, a region where half the population of 10 million people live within 1.5 km of the sea, few will be untouched by the consequences of climate change, including ocean warming, sea level rise, more frequent tropical storms, flash floods and droughts. Despite contributing a negligible 0.03% to global greenhouse gas emissions, the 5th assessment report of the IPCC identifies the 22 Pacific small island developing states as being the most vulnerable countries in the world to the adverse impacts of climate change. For example, Kiribati, comprising 33 low-lying atolls with a population of just over 108,000, could witness a maximum sea level rise of 0.6 m and an increase in surface air temperature of 2.9°C by 2090, according to the Pacific Climate Change Science Program. The country is experiencing higher tides every year, but can ill afford shoreline erosion, with a population density of 15,000 people per km 2 in some areas.Across the region, the Asian Development Bank estimates that the impacts of climate change could cost up to 12.7% of annual GDP by the end of the 21st century and are already causing dramatic revenue loss in agriculture, water resources, forestry, tourism and other related sectors. In 2012, Cyclone Evan was estimated to have caused damage equal to approximately 30% of Samoa's GDP; Cyclone Pam, in March 2015, is estimated to have displaced around 70% of Vanuatu's population, and is expected to have caused even greater economic damage.Higher temperatures, rises in sea level, and increased hurricane intensity also threaten the lives, property and livelihoods of 40 million people throughout the Caribbean region as a result of global warming. For example, rainfall records averaged across the Caribbean region for 100 years show a consistent reduction in rainfall according to the latest IPCC report, a trend that is projected to continue, signalling a significant threat to agriculture and water availability. In addition, the abundance of coral species is in rapid decline and has decreased by over 80% on many Caribbean reefs. Studies show that the projected costs to the region due to increased hurricane damage, loss of revenue to the tourism sector and damage to infrastructure as a result of climate change could be €7.96 billion by 2025, and €17.5 billion by 2050, according to the Caribbean Community Climate Change Centre. Such losses could cause an irreversible economic recession in each of the Caribbean Community member states. So where do we go from here? How are ACP countries doing business in a time of a changing climate and how will they continue to react and adapt in the decades to come as the impacts of global warming increase? In particular, how do small Pacific and Caribbean islands cope with the phenomena that threaten, in some cases, their very survival? And beyond the threats, are there also opportunities related to climate change for smallholder farmers and fishers? Some responses are provided in the case studies in Chapter 2, Fighting back: case studies from ACP regions.Climate-smart agriculture (CSA) -defined as 'agriculture that sustainably increases productivity, resilience (adaptation), reduces/removes GHGs (mitigation), and enhances achievement of national food security and development goals -represents a significant approach to achieving short-and-long-term agricultural development priorities in the face of climate change and serves as a bridge to meeting other development priorities. Launched in 2015, the Global Alliance for CSA will help support countries and other actors in securing the necessary policy, technical and financial conditions to enable the triple win (food security, adaptation and mitigation) that can be achieved through CSA approaches. Examples of making agriculture climate-smart are presented in Chapter 3, Making agriculture climate-smart.In 2015, governments will aim to agree on a new sustainable development framework that includes a set of longer-term Sustainable Development Goals, a future climate change agreement under the UNFCCC, and a post-2015 framework to address disaster risks. Collectively, these processes will provide a unique opportunity to fundamentally shift course towards global and national climate-resilient development pathways. However, whether these actions promote food and nutrition security in the face of climate change will be one of the key benchmarks in assessing success; six issues will be critical to this. These including mobilising financial support needed to scale up proven action and practice; ensuring equitable outcomes for women; giving decision-making power to farmers; enhancing nutrition security, not just food security; making mitigation an opportunity for, rather than a threat to food security; and supporting markets and value chains for low income producers and consumers. One of the greatest challenges, for example, will be how to ensure increased investment in sustainable, productive, equitable and resilient agriculture, through climate and agriculture finance. Further steps for the future are outlined in Chapter 4, What's next?Uncertainty in climate projections occurs from three principal sources:• Natural internal variability of the climate system -These are the natural internal and ongoing processes within the climate system and are largely independent of climate change processes.• Model uncertainty -IPCC uses a range of different General Circulation Models (GCM's) to project plausible future climate scenarios. However, there are still limitations in our knowledge of the process that govern the climate system coupled with limited computing resources. As a result, the models are imperfect and climate change projections vary, depending on which model is used.• Emission scenario uncertainty -climate projections derived from GCM's are based on greenhouse gas emission scenarios called Representative Concentration Pathways (RCP). These are created based on assumptions on how future GHG emissions will evolve. As there is considerable uncertainty associated with these assumptions, there is also uncertainty over which RCP is most likely to correctly representthefuture. As a result of these factors, there is associated uncertainty in projected temperature and rainfall changes (see Table I). Nevertheless, warming of the climate system is unequivocal, and since the 1950s, many of the observed changes are unprecedented over decades to millennia. The atmosphere and ocean have warmed, the amounts of snow and ice have diminished, sea level has risen, and the concentrations of greenhouse gases have increased (see IPCC 2013: summary for policymakers http://tinyurl.com/l8w5g5l).How important are fisheries in terms of livelihoods and economic security in the Pacific?Fisheries are not just important for families who rely on the reef; they also provide vital foreign exchange from tuna fishing in the exclusive economic zone (EEZ), where islands have jurisdiction over their resources. Kiribati and Tuvalu are the most fishery-dependent nations in the region, with fisheries contributing the majority of their government revenue and livelihoods. Fisheries also provide resilience against natural disasters and climatic events. Provided they remain productive, they are a reliable source of food when crops are wiped out. After a cyclone, island people go fishing.The greatest challenges are from coastal overfishing and the impact of urbanisation and agriculture on coastal habitats, which are very significant in some places. In terms of climate change, we have not yet experienced economic impacts from latitudinal shifts in species, but the main concern is about habitat decline caused by coral death. Reef fish populations do not migrate easily from island to island. Each island is effectively a mountain-top so reef fish cannot move to higher latitudes like continental shelf or pelagic (ocean surface) fish can.In oceanic tuna fisheries, we do not yet see notable challenges as a direct result of climate change because pelagic fisheries are already greatly affected by natural climatic cycles. People are accustomed to skipjack tuna becoming more available in the east of the region during El Niño years, for example.If the centre of abundance of skipjack tuna stock moves with the western tropical Pacific \"warm pool\" to the east over the course of the century, as the models suggest, there will be obvious winners in the east and losers in the west. However, if there are catastrophic reversals in major oceanic currents, rather than incremental change, then all bets will be off. Imagine what would happen to north-western Europe if the Gulf Stream suddenly shut down.My view is that unless Pacific Islanders are able to effectively deal with threats from coastal overfishing and land-based impacts, for example by promoting community controls with strong government backup, there probably won't be any reef fishing businesses left for climate change to affect. Diversification to small-pond aquaculture and to pelagic fisheries beyond the reef can help maintain fish-based livelihoods if reef fisheries decline. But, as they decline, the market price of reef fish will increase. So, for many reef fishers, there will be no economic reason to shift their business activities towards lower-priced aquaculture or oceanic fish until the reef fishery completely collapses. This presents a dilemma for policymakers.So what policy approaches are having an impact to help reduce the threat of climate change on this vital sector?For highly migratory stocks, one approach is for Pacific Island administrations to establish collaborative, rights-based management systems over the tuna fisheries in their own combined EEZs -systems that allow them to trade fishing opportunities among themselves, and so buffer the economic impacts of climatic changes. For example, under the Vessel Days Scheme, Kiribati can sell fishing days to Pacific Island states further west during La Niña events to maintain a more stable economy. This scheme has also enabled Pacific Small Island Developing States (SIDS) to properly value their tuna fisheries and obtain a much better return from the same level of fishing effort. Flag States (where vessels are registered) have traditionally held the balance of power in tuna fisheries, and establishing the rights of Pacific SIDS to govern tuna fishing in their own EEZs has taken many years. Even now, some distant-water fishing nations question these collaborative, zone-based management systems.Fishing is the mainstay of life on smaller, low-lying atolls of the Pacific, which are more dependent on fisheries than the larger, higher islands, where agriculture is possible. So what impacts will climate change have on this vital resource and the livelihoods that depend on it? Tim Adams has spent all his working life in Pacific Island fisheries and is currently director of fisheries management at the Pacific Islands Forum Fisheries Agency. His goal in life is to see Pacific Islanders obtaining maximum benefit from the control of well-managed sustainable fisheries in their own waters, whether at the national level in tuna fisheries or at the village level in reef fisheries.\"We sense the onset of climate change, especially in terms of rainfall, not the winds. We can't talk about drought per se, but rainfall is now irregular and, especially, it's unpredictable,\" said a woman cocoa farmer at the last Agriculture and Animal Resources Fair held in mid-April in Abidjan. \"We used to be able to predict volumes of inputs we would require and draw up a timetable for applying them because we knew the rains would begin in June and last until August or September. But this is no longer the case and now we can't make forecasts.\"This woman, member of a cocoa growers' group, cultivates cocoa trees around Soubré, capital of Nawa region in southwestern Côte d'Ivoire. As the world's leading cocoa producer, this country alone supplies 40% of the cocoa beans marketed worldwide. Côte d'Ivoire therefore has a key role in this sector, especially since the global demand for chocolate is steadily growing and there are fears that cocoa supplies will not be sufficient to meet this demand.The research findings are indeed alarming. According to the International Fund for Agricultural Development, the area devoted to cocoa production worldwide could shrink by 20% by 2050. In Côte d'Ivoire, a temperature increase of 2.3°C in key cocoa growing regions such as Moyen-Comé, Sud-Comoé and Agneby would have a major impact on productivity, according to a study by the International Center for Tropical Agriculture. Cocoa trees are more susceptible to the climatic drying trend than to the rise in temperature because dryness leads to increased evapotranspiration due to the rising temperatures, but this moisture loss is not offset by higher annual rainfall, which is actually decreasing slightly, reports the French agricultural research centre CIRAD.One solution lies in agroforestry. Cocoa trees grown in an agroforestry environment can, according to CIRAD, produce up to 1,100 kg/ha of cocoa beans, with the cocoa tree longevity sometimes exceeding 50 years, as compared to 300-400 kg/ha and 20-30 years for monocropped cocoa trees in West Africa.\"We're working with different partners, such as the Coffee and Cocoa Council (CCC), to overcome this problem via forest tree replanting. This initiative provides shade in the plantations and helps generate rainfall. The CCC advises us to plant these trees because cocoa requires water and shade,\" explains the woman coffee grower. This is a major shift in strategy since cocoa and coffee cropping are known to be responsible for the loss of some 30 million ha of primary and secondary forests worldwide, while also generating greenhouse gas emissions. But this negative trend could be reversed by planting trees on the 20 million ha currently cropped with cocoa and coffee.There has also been a major turnaround in individual grower's practices -farmers have been clearing land to plant cocoa trees for decades in Côte d'Ivoire, so the positive relationship between forest trees and cocoa trees has not always been evident to them. But now these farmers are increasingly aware of the positive benefits of shading their cocoa trees during drought periods, which also improves soil fertility. The Forest Development Corporation in Côte d'Ivoire offers growers access to forest tree seedlings. Cocoa growers are also often eager to grow fruit trees (orange, bush plum, avocado and cola) as cover and as a source of food and income.Implementing this agroforestry plan is often complicated and the results could be better. According to François Ruf at CIRAD, \"one environmental recommendation is to keep 12 native forest species per hectare of cocoa, while ensuring that on this area 18-25 trees are providing sufficient cover above the cocoa tree canopy so that 30-40% of the plantation is under shade. But nobody really complies with this recommendation even though some growers do claim that climate change has prompted them to maintain at least partial shading in their plantations. In fact these growers generally only have around two forest trees per hectare that provide cover above the cocoa tree canopy.\"Nothing is perfect, but it is now certain that cocoa is a shade-loving tree.Cocoa growers in Côte d'Ivoire are aware of the highly positive impact of growing cocoa under shade to cope with climate change. Trees are consequently being replanted where at one time farmers cleared the land to plant precious beans.In Côte d'Ivoire, a temperature increase of 2.3°C in key cocoa growing regions will have a major impact on productivityThe most significant consequences are happening as a result of rising temperatures, with the greatest effects seen in the poultry, dairy and pig industries and to a lesser extent with small ruminants. In relation to the poultry sector, adverse effects include higher mortality and reduced productivity. In the dairy sector, effects include poorer feed conversion and reduced milk production.A number of short-term measures are being employed by farmers. Tunnel house ventilation, which assists in managing heat stress, is becoming mandatory in order for broiler enterprises to be successful. Similarly, dairy farmers have been managing heat stress by keeping animals under shade at all times during the day. As a result, cut and carry methods of bringing feed to the animal, or even allowing them to feed at night, are now common practice.Meanwhile, scientists are examining breeds that are adaptable to the changing climate, particularly with ruminant livestock. The aim is to exploit local indigenous breeds such as the Blackbelly sheep, given the fact that we, as a region, have a history of importing exotic breeds that are not adaptable. Currently, imported breeds such as Dorper and Katahdin sheep have shown significant signs of heat stress. How are regional organisations getting involved to help livestock businesses cope with the impacts of climate change?The Caribbean Agricultural Research and Development Institute is at the forefront of efforts in relation to climate and agriculture. With livestock feed, the Institute is researching cultivars and forages that farmers can use to combat the problem. It also has a limited involvement in poultry and pig research, although generally these industries are supported by industry stakeholders, such as the Caribbean Poultry Association. Livestock scientists at The University of the West Indies are undertaking research, especially in relation to poultry production and small ruminants. This is particularly focused on what measures and best practices can be used to manage heat stress.What still needs to be done with research or support for farmers to help livestock businesses?Long-term solutions are important and the best bet is to improve resilience in livestock breeds. We should definitely not be importing exotic breeds to upgrade our animals. Prioritisation of genetics to develop breeds that are more resilient to climate change is paramount. According to our prediction models, the Caribbean region will have longer droughts and more intense storms and we must therefore ensure that local animals are able to withstand these types of stress. There is also a need for improved housing to protect livestock from heavy trade winds and solar radiation, as well as to ensure adequate space for each animal. Lastly, we must continuously assess and ensure that we have sufficient feed resources for our livestock during times of further climatic change.How do you feel about the future of livestock businesses in the Caribbean and other small island developing states in the years to come?While some areas are much more vulnerable than others, a major cyclone like the recent one in the Pacific can completely devastate a livestock industry. Some livestock businesses, such as poultry, can be restarted fairly quickly; others, such as the Caribbean's indigenous sheep and goats, will be quite difficult to restart if lost. For the future of the livestock industry, yes, there are risks that we need to manage.Rising temperatures and more intense storms pose a significant threat to livestock health and productivity. Using genetics to improve indigenous livestock breeds, adopting practical strategies to manage heat stress, and developing climate resistant forages are some of the strategies that will be needed to build climate resilience in the sector.Norman Gibson is a livestock scientist and commodity leader for small ruminants at the Caribbean Agricultural Research and Development Institute in Trinidad and Tobago.Whilst farmers across ACP regions are undoubtedly being impacted by global warming and climatic changes, there are a notable number of positive initiatives where individuals and groups are working hard to overcome the challenges they face.Climate change has had an impact near Kumbo in northwestern Cameroon for several years. Although in many cases the communities affected are only aware of climate change because of media coverage.\"The rains didn't start when expected. This year we got heavy rain for a week and then not a drop for the next 3 weeks. Traditional annual crops like beans, maize and Irish potatoes are feeling the strain as they are very susceptible to excessive rain and sunshine,\" says Gilbert Njodzeka, coordinator of the NGO, Green Care.The responsibilities of fetching water, tilling the ground and sowing crops mostly fall to women, who are often the most affected by changing climate conditions. Women produce up to 80% of the food used for household consumption and sold in local markets in sub-Saharan Africa, according to the World Bank. Over the last 10 years, Njodzeka has therefore looked to women to initiate new projects and grow different crops near Kumbo. \"It is women who make the difference,\" he says. Since 2004, women in the region have been planting trees rather than cutting them down, in particular the adapted native species such as acacia, calliandra, croton, and Polyscias fulva. Plants are provided free of charge from nurseries set up by Green Care. Tree planting is a festive occasion where women can prepare meals, sing and dance, which \"is a way to get them engaged,\" says Njodzeka.Once the trees start growing, women are quickly aware of the benefits of agroforestry and request more plants. \"Our part of the work is then complete,\" Njodzeka says. This has also prompted the growing of trees for hedges. \"The shade from trees allows for green pastures during dry spells, while the branches can be broken and fed to poultry and small livestock, such as rabbits. The trees nourish the soil, livestock and provide shade.\"Beekeeping was once an overlooked traditional activity, in particular due to the lack of special protective clothing required. However, in recent years beekeeping has been revived among women in northwestern Cameroon.Green Care has provided equipment and training for women to plant trees that attract bees, which has increased productivity, the quality of honey produced, and as a result their incomes. This is a win-win solution from both an environmental and economic standpoint. Bees are essential in the pollination process -a prerequisite for flower, fruit and vegetable cultivation. A share of the honey produced is currently sold on regional markets and women have been asking Green Care to help expand to other remunerative markets.In an ongoing effort to find crops that are less susceptible and more resistant to climate change, while generating income, Green Care, with the support of the US Peace Corps, has identified soybean's potential. \"Soybean is not a traditional crop in our region, but it has been introduced and is thriving. Women are aware that this crop requires little water and has proven qualities as a source of protein. Soybean also adds value because it can be processed into flour, oil or oil cake for livestock feed. Farmers can rely on this crop, where other less resistant crops often suffer,\" specifies Njodzeka.Mushrooms are also a climate changeresilient option. Water-efficient as they are easy to harvest, prepare and offer substantial additional income for women, who dry the mushrooms before selling them on the market.Women in northern Cameroon are growing new crops using innovative cultivation practices to manage changing climate conditions. This pilot project was designed to cover the entire value chain to effectively address the issues from all angles. Three 'dialogue theatres' on climate risk were conducted at the production, processing and marketing levels, which involved role playing games to raise everyone's awareness on the functions of the other participants and their climate change coping strategies.Most stakeholders are already taking action, i.e. intercropping, agroforestry, irrigation and debt rescheduling; but almost all of these initiatives are uncoordinated. So everyone passes the buck when coffee prices drop -the farmer blames the input supplier, who then underlines the grower's inability to properly negotiate with the intermediary, who in turn contends that the grower is trading coffee of degraded quality, while the exporter claims that the low prices are due to the lack of rigour along the value chain.This absence of communication and confidence undermines collective attempts to adapt to adverse environmental conditions. However, coffee growers are more vulnerable than any other stakeholder since they have few alternatives to growing coffee and limited organisational capacities, they are therefore hard hit by sliding coffee prices. The pilot project revealed that it is essential to reduce the number of intermediaries (exporters purchase 70% of their coffee from traders) in order to enhance communication between stakeholders, develop contract farming and strengthen growers' associations so that members will have more bargaining power when climate hazards occur.Improved networking between stakeholders and the development of interpersonal dynamics have raised the awareness of public authorities. \"Under the National Agricultural Advisory Services (NAADS) programme, the government distributed drought-resistant coffee plants and funded simple irrigation schemes especially. These initiatives are ongoing under the government's Wealth Creation Programme -a follow up to NAADS,\" says Norman Ojamuge, a ministry representative.Money is the lifeblood that is lacking. Investment is necessary throughout the value chain, which implies incorporating climate risk in funding mechanisms, while developing warehouse receipts and climate-indexed insurance. The Centenary Rural Development Bank manages a credit project based on warehouse receipts and offers credit in the form of inputs rather than money, with growers in 10 coffee producing regions already benefitting.Following this project, improvements in transport, irrigation and warehouse infrastructures were identified as a priority in the Uganda Coffee Sector National Export Strategy 2012-2017 Update.Climate change has a broad range of impacts on different stakeholders throughout the value chain; everyone must find a suitable coping strategy. The extent of resilience to climate change is dependent on responses to these impacts, from the production stage to the final product.Dialogue theatres help to raise awareness on the roles of other stakeholders and their climate change coping strategies © IISD In Ghana, extreme weather events are becoming increasingly frequent, leading to loss of crops and falling income levels for many households. This is placing farmers, especially small-scale farmers, in a precarious position, with those living in the three northern regions particularly vulnerable. In this challenging context, the use of interactive radio has been helping to create links between farmers and markets, and is providing farmers with much needed information about climate and local weather, and ways of strengthening their farming systems to cope with the effects of climate change.A small number of organisations are using ICTs in Ghana to provide climate or marketing advisory services to farmers. These include Esoko, the International Institute for Communication and Development and Grameen. Farm Radio International (FRI), for example, in collaboration with two of its radio broadcasting partners (Akeaa FM and Obouba FM), supported production of an 8 month interactive radio series. This was aimed at helping small-scale farmers in the Ashanti Region to produce sufficient quality and quantity of maize and cowpea for home consumption and sale to potential buyers, including premium markets like the World Food Programme's (WFP) Purchase 4 Progress (P4P) initiative. The radio series reinforced training workshops delivered by WFP on good agronomic and postharvest practices and quality standards. Programming on climate change has also been delivered in recent years by the Dr. Adaptation radio shows, where farmers are given advice on how to avoid damage to their farms from severe weather. Both radio series have introduced a number of ICT tools in order to facilitate farmers' participation and engagement, both with the radio broadcasters and each other.One key participatory technology is an interactive voice response system. This system allows farmers to access important messages and alerts, to listen again to radio programme segments, and to record and share messages with radio stations, such as lessons they have learned in the field from implementing new methods. FRI provides training and mobile phones to selected farmers' organisations, enabling their members to access spoken information, including market prices, weather information and agricultural tips. The content is provided by the Ghana Meteorological Agency, a market information agency and the Ministry of Food and Agriculture; it is then recorded by FRI and sent to the radio stations. Other ICTs introduced by FRI include a 'Beep2Vote' system, which allows listeners to vote either yes or no on a chosen issue, using a text number. Answers are tallied through a 'Telerivet' system, presenting the results to the broadcaster in graphic form and allowing him or her to comment on them within the programme. SMS updates are used to inform listener group leaders about the time and topics of weekly programmes.Mr Abdul Raman Yangah, a member of the Nkwariedee Farmers' Association, emphasises the value of the radio programmes to his business: \"The radio programme teaches us the best way to harvest and store our produce. One thing that we are happy about... is receiving weather updates -this has helped us to know when to spray our farms so we don't run at a loss.\" Information delivered by radio and mobile phone services has helped farmers to understand and prepare for climate change on their farms. The P4P initiative is also providing valuable food reserves for distribution in times of need, a support system which will become increasingly important as some regions experience severe or prolonged drought due to changing climatic conditions. Hundreds of thousands of farmers in Ghana are now improving their resilience to climate change, through listening to the radio and using their mobile phones to access the information they need, when they need it. Whilst the death toll was mercifully low, for the young people of Vanuatu the cyclone's impact will not be easily forgotten, with most homes and infrastructure also destroyed.The impacts of a changing climate in the Pacific will not always be as severe as witnessed in Vanuatu. However, for many islanders, soil erosion of foreshore areas, regular flooding, and saltwater intrusion are evidence of a significant threat. For the Pacific youth who are growing up experiencing these changes and their impact on food security, understanding the challenges related to global warming is an increasingly important priority. At the 3rd International Conference of Small Island Developing States in September 2014, 29 young people representing Pacific youth from 11 island nations stated: \"We would like to see environmental issues brought to the fore in the school system to increase awareness of their effects and the role young people can play. We are committed to taking a more active role in the provision of community outreach programmes.\" To provide platforms for children and young people's voices to be heard in environmental decisionmaking processes, Project Survival Pacific -Fiji's youth climate movement -has been working with young people from across the Pacific since its launch in 2008. The Youth Climate Ambassador Program is a key activity, training young people aged 18-30 to become Fiji's youth climate change representatives to international forums, where they work alongside Fiji's national leaders in steering the direction for international policies. For example, environmental sciences graduate Devika Raj was the Youth Climate Change Ambassador to the COP18 climate change negotiations in Doha in 2012. \"I believe climate change is about more than environmental effects; it is a threat to human rights, particularly for us in the Pacific who contribute little to global emissions, yet are feeling its consequences first and hardest,\" states Raj. She continues to be an active spokesperson, writing in local newspapers, talking to young people, and getting involved in climate policy work.Executive director of Project Survival Pacific, Krishneil Narayan, is committed to passing on skills to young people so that, \"they are better equipped to innovate and adapt to the changes they are seeing.\" During 2015, climate change will be integrated into Fiji's school curriculum for primary and secondary levels. As part of his organisation's efforts to support this, he has been visiting rural high schools in Fiji to conduct 'Climate change: Fitting the pieces together' introduction sessions. \"No-one is too young to voice their concerns on what is happening to our environment,\" says the project's community outreach coordinator, Sula Muletanavanua. \"Fijian youths should stand up and take action on climate change because what we do today will be our future.\"However, the effective inclusion of climate change in the curriculum is not possible unless teachers are also trained and have the available resources. In Samoa, climate change materials have been recently introduced by GIZ, the German development organisation, in collaboration with regional partners, to support teachers in integrating climate change into social studies and science curricula for 12-13 year olds. Training workshops for teachers, held in late 2014/ early 2015, revealed that there was a basic lack of climate science knowledge amongst many of the teaching staff. Since the training, Ruta Seumanutafa of Falealili College has been teaching her Year 9 social studies students about conserving water: \"The training resources will help my students learn to save water at school and at home. And, as a pastor's wife, I'll be able to pass this information on to my church.\"Enabling youth in the Pacific to be influential, motivated and engaged in solutions to climate change is the mission of Fiji's youth climate movement, Project Survival Pacific. This aims to ensure that young Pacific islanders can have an active voice in decisions that will impact on their future survival.Project Survival Pacific -Fiji's youth climate movement -works with school children and young people from across the Pacific More than 90% of Guyana's population lives and works today on the low-lying coastal zone, making their daily life vulnerable to rising sea level due to global warming. The sea level is projected to rise by 1 cm per year or 40 to 60 cm by the end of the century, according to the Ministry of Agriculture. People still recall 2005, when most of Georgetown and several outlying areas were flooded due to unusually high rainfall: 132cm of rain fell in 2 days, the highest level since 1888! Guyana is confronting these climate change challenges with massive programmes aimed at strengthening its sea defense, creating a reliable internal drainage system and relocating its food production to higher lands. Permanent secretary in the agriculture ministry George Jervis says the country has an ambitious plan: \"We estimate about €135 million is required to provide better infrastructure on the coast.It includes keeping those drainage channels (to the Atlantic Ocean) clean, adding pumping capacity, pumps and pump stations, shoring up the dams of the East Demerara Water Conservancy, maintaining and cleaning its holding capacity as well as putting another outlet to discharge excess water from the conservancy.\"In In 2014, €1.7 billion of Guyana's national budget was allocated to ensure the further strengthening of the sea and river defense infrastructure. The EU is the only external agency that directly helped Guyana with €14.8 million from the 10th European Development Fund allocated towards sea and river defense rehabilitative works and capacity building in Guyana. These funds, amongst other projects, allowed the Mangrove Restoration Project which aimed to use a natural sea barrier to protect the country's coast. In 2014, more than 50,000 seedlings were planted.The country is affected by floods but also by droughts. In the Rupununi in the south of Guyana, one small ranch is experimenting with water harvesting. \"The Rupununi is usually affected by extreme floods or extreme droughts but J and R Ranch is experimenting with a system to trap water over a six month period for agricultural purposes\", Jervis said. He explained that the government wants to promote this model and is seeking funding to do so.Guyana is also experimenting with the relocation of its agricultural production away from the flood prone coastal zone. The targeted areas are located in the Intermediate Savannahs, 95km from the Berbice River, the country's second largest river, the Soesdyke/Linden Highway and the Rupununi Savannahs, close to its border with Brazil.Several companies are assessing the suitability of the soil in the Intermediate Savannahs for growing soya, corn and sugar cane. But George Jervis explains that currently, poor infrastructure, such as access to inputs and markets may be discouraging local farmers from securing land in that location.Root vegetables and fruits are being grown by private farms located on the Soesdyke/Linden Highway. It is where, following the 2005 floods, some farmers wanted to move; they could have secured land through leases. However, as Jervis explains, while not many farmers have relocated, many new farmers are now occupying land on the Soesdyke/Linden Highway.\"The highway has become a central area for root vegetables, especially the highly nutritious eddoes. In the past such root vegetables came from riverain communities but those communities have become prone to flooding because of climate change.\"In rice producing areas close to the sea, experts are developing salt tolerant varieties.\"Because we expect sea level rise, the plant breeders are developing varieties that are able to tolerate salt water and survive for longer periods when flooded, so we are working on that\", George Jervis said.Guyana's dramatic floods in 2005 highlighted changing climatic conditions and increased the pressure on the government to take steps, with the help of donors, to protect its coastal zone and agricultural areas.The Global Alliance for Climate-Smart Agriculture (GACSA) -open to a broad range of stakeholders -was launched in September 2014 at the UN Climate Summit in New York to strengthen food and nutritional security during climate change. The first meeting was held on 17-18 December 2014 in Rome. GACSA governance includes a top level annual forum, a strategic committee overseen by two co-chairs (currently the New Partnership for Africa's Development and Norway), a facilitation unit hosted by FAO, and action groups (knowledge sharing, access to funding, creation of a conducive environment). In early 2015, GACSA had 75 members from various backgrounds (including CGIAR, Danone, the International Union for Conservation of Nature, the Niger Government and the World Bank). Civil society has levelled three main criticisms against this alliance, challenging its governance mechanisms, transparency and accountability. Coordination SUD's Agriculture and Food Committee expressed its concerns via Patrice Burger of the French NGO, CARI, who fears that in the currently uncertain setting (\"a yet to be formed global coalition under a yet to be defined concept\"), some parties may try to turn the situation to their advantage, particularly by promoting biotechnology. Meanwhile Patrick Caron of the French Agricultural Research Centre for International Development (CIRAD), a GACSA member, believes in this tool. To those fearing a shift towards promotion of industrial production systems that are detrimental to family farming and the environment, he responds, \"CSA does not intend to work on promoting a model, but instead offers a forum for debate that was previously lacking. It is a mainstay in the preparation of the agreement to be signed at the Paris climate conference in 2015.\"H ighly vulnerable farmers in ACP countries are currently frontline victims of the climate change process. This is sadly illustrated by the recent human and natural devastation wrought in the wake of Cyclone Pam, which tracked through Vanuatu on 18 March 2015. Farmers have to deal with major challenges in their livelihoods, producing more for an ever growing number of people, adapting to climate change and reducing their climate footprint by curbing their greenhouse gas (GHG) emissions.Agriculture is responsible for 24% of global emissions. CSA shoulders the expectations of many farmers wishing to change their practices to address these challenges. The concept has taken tangible shape over the last 5 years but there are still many unknowns. Major changes will be necessary in production systems, as well as in consumption habits in both developed and developing countries. Some stakeholders still question the motives and practices that have raised CSA hopes; a few consider it as just a way to rehash old methods, while for others it is a genuine revolution. But despite the CSA successes noted here and there, the change of scale remains a major challenge. Political authorities, researchers, funding agencies and farmers are addressing the issue.CSA advocates changes in agricultural practices as well as the adoption of radically innovative technologies. This includes soil conservation techniques and land management strategies to enhance productivity, resilience and the carbon balance of agricultural systems in both developing and developed countries. CSA is devoted to updating, requalifying and promoting practices that have been used for generations, especially by family farmers, but which were pilloried during the Green Revolution. Assisted Natural Regeneration (ANR) was introduced in Niger in the 1980s and has become a typical example of the potential offered by CSA. In the last 30 years, over 5 million ha of land has been restored, with more than 200 million trees rejuvenated or planted. This has improved food security for around 2.5 million people, while strengthening the resilience of agricultural systems to extreme climatic events, diversifying farmers' sources of food and income and protecting land and water resources. ANR has also contributed to climate change mitigation through the sequestration of large quantities of carbon in soil, tree branches and roots.In the Solomon Islands, the Secretariat of the Pacific Regional Environment Programme promotes the use of environment-friendly agricultural production practices, such as improved pesticide and input use and the adoption of ecosystem protection and restoration practices and techniques. Soil protection can boost the organic matter content of soils and thus their carbon sequestration capacity and fertility. Ecosystem-based adaptation should be a key element of future climate change approaches in the Pacific region.Besides these representative examples, agroecology is now considered as a key element of CSA, as clearly outlined in the final declaration of the CSA 2015 conference held in Montpellier, France. Ecological concepts and principles are thus applied to design sustainable agroecosystems. In practice, agroecologyfriendly farmers strive to imitate nature in their fields, taking advantage of complementarities between different plants and animals. For instance, a Kenyan smallholder increased his milk production threefold, to l/day, by feeding his seven goats Napier grass (Pennisetum purpureum) and tick clover (Desmodium sp.). Moreover, it was found that intercropping maize with tick clover, which fixes nitrogen and repels stemborers, boosted maize yields from 1 to 3.5 t/ha.Research is being mobilised to develop new plant varieties that will help farmers maintain their crop yields despite climate change.Production of maize, a staple food for over 300 million Africans, could drop by 30% as a result of security, mitigation and adaptation). He feels that this new 'cornerstone' could reposition agriculturean important dimension that was overlooked by the UNFCCC -at the centre of negotiations on climate change, while providing access to specific funding.Specific policies accompanied by targeted funding are essential to uplift CSA from a few scattered projects to a fully-fledged global movement. In addition to the Green Climate Fund (€9 billion earmarked for addressing climate change, as announced by Ban Ki-Moon at the climate change conference in Lima (COP 20) in Lima, out of the annual budget of €91 billion promised at the Copenhagen Conference in 2009 to be allocated as of 2020), other funding arrangements are available; including payments for environmental services and carbon credits. Among these, the World Bank BioCarbon Fund is focused on land use and promoting landscape transformation to benefit poor farmers. This tool is used in the Kenya Agricultural Carbon Project supported by the Swedish NGO Vi Agroforestry, which helps farmers adopt sustainable agriculture management practices. So far some 15,000 farmers have adopted these practices, which are being applied on around 12,000 ha of degraded land. It is estimated that this will ensure an annual reduction in GHG emissions of 60,000 t of carbon dioxide equivalents, while restoring degraded land, enhancing yields and reducing farmers' vulnerability to climate change.Index-based insurance can also help farmers mitigate climate-related risks. In 2011-2012, through the adoption of such insurance systems, 29 million farmers in India were compensated for crop losses due to bad weather. This undeniable success story from India is often showcased, but keep in mind that it was set up with substantial political support (two-thirds of the project funding was provided by the government).Finally Jacob Owuor Onyango, a 30-year-old farmer from Lower Kamula CSV, has seen the impact of climate change. His farm is located by the Asao River, which once flowed continuously but now flows only during the long rains. Owuor used to grow irrigated tomatoes, but as rainfall and river levels became less certain his yields became increasingly unpredictable. Now, Owuor has developed a 'smart farm' on 0.1 ha of land, where a greenhouse, drip irrigation system and two water pans have been constructed. Owuor also chairs a youth group, teaching climate-smart practices with his farm as a village field school. Beginning with tomatoes, the group diversified into leafy vegetables, which they sell locally. Then, with the increased profits, they further diversified to rear rabbits and improved indigenous chickens. They have also changed their beekeeping practices, introducing Langstroth hives and doubling their honey harvest to 10 kg a year.Climate change has also affected livestock rearing in Nyando. Karen Onyango has, in recent years, found her goats and sheep performed poorly in drought periods and had low disease resilience. In 2012, the Nairobi-based International Livestock Research Institute introduced Galla goats and red Maasai sheep, both adapted to arid conditions, for cross-breeding with local animals. The female Galla has a long productive life and can breed for up to 10 years. Galla goats also mature 6 months earlier than local breeds and fetch €50 to €60 more in local markets.Besides livestock income, Onyango has also diversified to growing fast maturing indigenous vegetables, which earn her around €10 (Ksh 1000) per week during the rainy seasons. At planting time, Onyango relies on climate data sent to her cell phone by Maseno University and the Kenya Meteorological Service.\"They have trained us to make sense of weather information and know which crop varieties to grow according to the rainfall,\" she says. Onyango has also learned to intercrop her legumes and maize with agroforestry trees, which provide livestock fodder. And, since her farm is prone to soil erosion, Onyango also plants the soil-binding vetiver grass, provided by the Kenya Agricultural and Livestock Research Organisation.In 2011, 81% of households surveyed in Nyando experienced 1 to 2 months of hunger each year, with most surviving on one meal a day; only 1% of those surveyed were food secure throughout the year. Since the introduction of CSVs, 3% of the targeted population are now food secure for the whole year. And, with the CSVs' success, nearby farmers come to observe and learn, and are regularly gifted a plump, healthy goat or improved crop seeds so the benefits continue to spread. The Pacific island nation of Palau is witnessing the impact of climate change on a number of fronts. Increasing sea surface temperatures are causing changes in fish migration patterns, for example, leading to a fall in catch. Typhoons have also become more frequent, including typhoon Bopha in 2012 and typhoon Haiyan in 2013; changing climatic patterns have seemingly put the islands in the path of cyclones, where previously they had been spared. A third significant impact has been the saltwater inundation of Palau's taro plots, caused by sea level rise and extreme high tides.Taro is the most important crop in Palau's diet, and takes a central role in traditional events such as funerals and first birth ceremonies. The island has a wealth of taro varieties, but in recent years, saltwater intrusion has killed taro crops in nearly every state. Some farmers have been able to move their crops to higher ground, but most have nowhere else to cultivate and they have had to abandon farming this vital staple.In 2011, a pilot project was set up to evaluate tolerance and susceptibility of different taro varieties to saltwater intrusion. Sixteen local varieties of taro were tested on a small site in Ngimis, Ngatpang State, with the research finding three outstanding varieties that survived saltwater inundation and had an acceptable taste among people in the local community. The research was undertaken by a partnership between the Palau Community College Cooperative Research and Extension Department (PCC/ CRE), Palau Community Action Agency (PCAA) and representatives of a women's group and traditional leaders. According to Dr Aurora Rosario of the PCC/CRE, the saltwater resilient taros are now being tested in other states. \"We are hoping that as soon as we identify the taro varieties that are saltwater resilient, we can distribute and propagate them to farmers and make their abandoned areas productive again,\" Rosario says.Traditionally, many farmers in Palau have avoided upland cultivation, on account of the clay soils found there being unsuitable for crops. However, with the increasing danger of saltwater flooding in the lowlands, a second project has launched an upland agroforestry trial on an 11,721 m 2 area, also in Ngatpang State. In 2012, with the help of youth from Ngatpang village, fruit and timber tree species, including coconuts, mahogany, soursop, rambutan, avocado, tropical almond and banana were planted on the site, together with erosion-controlling lemongrass. Small quantities of NPK fertiliser were used to help establish the plants.The project was led by Leonardo Basilius, food production officer of the PCAA, who has been pleased to report that the lemongrass has grown vigorously. Calophyllum and mahogany trees planted in 2012 have also grown faster than expected. However, some areas of the site are showing signs of nutrient deficiency, and Basilius has recommended the planting of legumes to improve soil fertility and provide shade for some of the other species in their early stages of growth. As the pilot project came to an end early in 2015, the land has officially been handed back to Ngatpang State. However, Basilius plans to request the authorities allow him to use the site to train other farmers who are interested in going into upland cultivation.A third initiative has focused on mangrove crabs, a traditional part of the Palauan diet, which are also in high demand in hotels and restaurants. Crab populations have been falling in recent years, due to unsustainable harvesting and rising sea temperatures. In response, the Palau Community College Aquaculture Centre initiated a project in 2013 to supply small crabs to farmers and support them in rearing the crabs to a marketable size in submerged cages. In the same year, the hatchery also released nearly 400,000 crablets into the ocean at two conservation sites, in an attempt to boost mangrove crab populations.Community organisations are undertaking a number of initiatives in Palau to help local farmers and fisherfolk cope with the impacts of climate change. One project has identified saltwater-tolerant varieties of taro. A second project is experimenting with upland agroforestry, while a third is helping to boost populations of mangrove crabs hit by rising sea temperatures and overharvesting.To boost mangrove crab populations, the PCC Aquaculture Centre is supplying crablets for farmers to raise in cages in existing fishpondsWhat do you consider as the key ingredients to CSA today?CSA is broadly defined and covers a wide range of potential farming innovations spanning improved crop, soil, water, pasture, tree and livestock management initiatives. But to be termed as CSA, all must be related in one way or another to climate-induced risk or climate change. In other words, the key ingredients of CSA innovations include either one or a combination of the following: (i) climate-induced risk management and or reduction, (ii) advanced preparation for future climate and (iii) mitigating climate change through carbon sequestration into soils and vegetation. Also where possible, reduced emission of greenhouses gases such as nitrous oxide and methane. And of course, such innovations also need to contribute to achieving of national food security and development goals.For small-scale farmers, given the urgency of climate change, should the priority be given to coping or adaptation strategies? Or both?Priority needs to be given to both, but in different contexts and different timescales. There is clear evidence, on a global scale, that surface temperatures are increasing and emerging evidence that rainfall amounts and distribution patterns are beginning to change. However, as yet, the impacts of these changes are still relatively slight. In the shorter term therefore, priority must continue to be given to helping farmers improve the productivity and sustainability of their traditional coping strategies, which have evolved over generations through their experiences of natural day-to-day and season-to-season weather variability. However, by 2050 the next generation of farmers will likely be experiencing substantive temperature increases as well as important changes in rainfall characteristics. Priority should therefore also be given to the development and rigorous testing of strategies to help farmers adapt to these new climatic condition; conditions that they will not have experienced before.As you say, a huge amount of valuable research has been undertaken in ACP countries over the last four to five decades which has targeted improved agricultural production and livelihoods of farming communities. Yet widespread adoption remains stubbornly low. Current efforts to enhance the adoption of such farming practices must be radically reinforced if the world is to meet the increased food demands resulting from human population increases. It's no use just assuming that the world will always be able to feed itself; it has to be made to happen and that must therefore remain the current development priority. An added benefit is that not only will such efforts help farmers now, but in many instances will continue to provide added value to future generations, even under changing climates. But we also need research that is forward looking and targeted towards limiting the negative impact that changing climates will have on agriculture in many ACP regions. Of course, there are recognised uncertainties associated with climate change projections, especially in rainfall amounts. This adds complexity to adaptation research. However, all the used models agree that the Earth's surface will continue to warm up beyond 2050. In that respect, I believe that crop and livestock breeding for higher temperature tolerance has a vital role to play. It is also projected with a high degree of confidence that extreme weather events will increase in frequency and severity. Research that generates improved forecasting and allows better preparedness for such events is vital. So is research that identifies the most appropriate weather-based insurance schemes for crops and livestock; this will help farming families survive when extreme events and seasons occur. I would also like to see greater use of crop growth simulation models in ACP countries. Such models are becoming increasingly accurate and, when used with appropriate weather generators, can provide realistic ex ante simulations of the performance of a wide range of soil, water and crop management practices under future climates. Such research does not, of course, replace field-based research, but is invaluable in helping to refine research priorities and field testing targets.\"There is a twin urgency associated with Climate Smart Agriculture (CSA)\" says Peter Cooper. \"The urgency to develop and disseminate CSA to help this generation of farmers cope better with current climate variability, but also the urgency of preparing for climate change with new CSA innovations that will allow future generations to adapt their farming practices.\" Adaptation to climate imbalances or mitigation via carbon sequestration -agriculture is currently viewed as both a solution and a problem. African governments, the scientific community, development agencies, civil society and private enterprises are rallying to the cause, but a lot more remains to be done.The Pacific Islands have prioritised the development of small-scale renewable energy production, initially to complement and eventually to replace existing sources. Tokelau is a tiny island nation with a population of 1,500, spread across three coral atolls. Before 2012, the islands' electricity was supplied by three small power stations, fuelled by 200 l of diesel per day, imported from New Zealand. This was sufficient for 15-18 hours of electricity supply each day, at a cost of €750,000 per year. However, at COP 17 in Durban, Foua Toloa, the former head of government of Tokelau, said the islands would be using 100% renewable energy by 2012 and, in October that year, Tokelau achieved its goal, becoming the first country to produce almost all its electricity from solar power; in overcast weather, the generators run on local coconut oil, providing power while recharging the battery bank.A ccording to Africa's Adaptation Gap 2, the UNEP's latest technical report published in 2015, if the global temperature rise remains below 2°C, annual adaptation costs for Africa could reach €45 billion by 2050, but this figure could double if there is a 4°C climb. Crop yields on the continent could also decline by 17% for wheat, 15% for sorghum and 10% for millet within the same timeframe, with an even more significant impact in Sahelian countries.At the 3rd Global Science Conference on Climate-Smart Agriculture held in Montpellier, France in March 2015, 700 researchers and development experts from 75 countries highlighted the need to set up earlywarning systems, develop agro-ecology research, promote family farming and local agricultural research, and to bridge gaps between disciplines.\"The physiological conditions for production that will prevail in Africa over the next decades -temperature levels, atmospheric carbon concentrations, humidity or soil minerals -are still completely unknown. Sound research will therefore be required to gain insight into how climate change will affect plants and animals. Powerful action and development mechanisms will also have to be planned and set up,\" points out Patrick Caron, chief operating officer for research and strategy at the French Agricultural Research Centre for International Development (CIRAD). Global research -a further challenge -should also be combined with local research to take specific local context into consideration.Researchers are upgrading and adapting agricultural techniques to be able to maintain and increase production in a changing environment. Agriculture is also expected to provide ecosystem services, reduce the impact of greenhouse gases, enhance biodiversity, maintain water quality, and regenerate soil fertility. \"Agriculture, underpinned by chemistrybased techniques, has undergone a remarkable boom. Now ecological intensification is being reinvented. We depend on making effective use of ecological cycles which enable us to produce more and better while considering crops in environmental terms,\" says Caron. Biodiversity and agroforestry techniques have thus been rediscovered and implemented to take advantage of the complementarity of relatively deep root systems, reduce the impact of diseases and maximise the growth of one species via another -ancestral knowledge put to effective use through modern techniques.Half-moons, stone bunds and zai... \"Farmers have cleverly managed to save useful species and continue to cultivate them.\" Practices used to ensure flood protection and water retention in many Sahelian countries include half-moons, permeable dikes and stone bunds. The traditional zai technique is now widely used in Burkina Faso. \"Species that efficiently fix nitrogen, regenerate soils quicker and preserve soil moisture are now favoured along with crop rotations. These are practices that African farmers have used in the past. Let's continue to conduct research to address current challenges while focusing on the dissemination of practices, training and technology transfer,\" says Traoré.Private companies and NGOs are involved in projects geared towards developing so-called 'intelligent' agriculture, according to the concept outlined by FAO and backed by the founding of the Global Alliance for Climate-Smart Agriculture in 2014. Since 2013, the charitable Howard G. Buffet Foundation has been working with DuPont, the large American chemical company, and John Deere, the farming machinery manufacturer, to disseminate agricultural carbon sequestration techniques to smallholders in Ghana. DuPont has thus identified locally-adapted maize seed that can be associated with cover crops such as cowpea to increase productivity, curb erosion and enhance soil fertility. Meanwhile, John Deere is testing no-till cropping techniques in collaboration with farmers.Research is currently focused on varieties resistant to extreme climatic conditions, on those with shorter cropping cycles and on controlling adapted pest insects, using biotechnology, traditional know-how (endogenous solutions) and modern techniques. The aim is also to foster the production and management of regional and national data on the climate and harvests, or access to satellite databases, which requires data acquisition and sharing regulations. Other promoted solutions include renewable energy use and the development of irrigation techniques to cope with drought (remote and drip irrigation). Morocco thus issued a funding request in early 2015 to the Union for the Mediterranean for an agricultural solar pumping project. This project is to include about a million Moroccan farmers with smallholdings of less than 5 ha, according to Hakima El Haite, the Moroccan Environment Minister.Research is well under way, but in the field there is a pressing need to include climate issues in national agricultural policies, build new development trajectories and coordinate regional policies. Land reforms are also called for. These needs are obvious in West Africa, for example, where Fulani herders traditionally wander with their livestock herds through the region during their seasonal © CCAFS/K Trautman Planning a sustainable irrigation system to maintain and increase production in a changing environment transhumance movements. Experts are approaching this highly controversial subject with caution. \"There is an urgent need for arable land as a result of the different drought cycles and population pressure. Areas earmarked for nomadic herders are no longer sufficient, which has led to border conflicts, as seen in Burkina Faso, Niger, and Togo. African states no longer have a choice; they are forced to draw up land legislation, which will challenge transhumance and the transboundary livestock herding concept. More herders will have to settle, but this represents a huge cultural change. Fulani life combines pastoralism, a traditional livelihood, culture and knowledge transfer. We must tread carefully with regard to this issue, starting from the ground and moving towards drawing up a policy,\" says Traoré.2015 is a busy year, and a turning point, with a meeting of the Subsidiary Body for Scientific and Technological Advice in Bonn in June, and the United Nations COP21 in Paris in December.Advances have already been achieved as the negotiating text makes several references to agriculture; and ACP countries can take advantage of this, which will avoid them having to bring the issues to the forefront on their own. But ACP countries are on tenterhooks as these major international conferences approach.In the Lifou Declaration, following the Oceania 21 Summit meeting held on 1 May 2015, Pacific Island countries underlined it is crucial that, \"our worries, sufferings, hopes and concrete proposals are heard by the negotiators.\" We are the living victims of the negative impacts of climate change. We are small, so we must speak with one voice,\" declared Fonotoe Pierre Lauofo, Deputy Prime Minister of Samoa.The Moroccan minister El Haite is worried about the post-2015 period.\"It is fine to design sound 'smart' carbon models, but we must have the capacity to set up and implement them and ensure their sustainability.\" This is a broad-ranging programme and hopes will be high in the months leading up to CoP21, but especially regarding the future.A Sahelian farmer using the traditional zai technique which is now widely used in Burkina FasoIn regions worldwide, millions of people depend on the media for its role in communicating information. This is true in the Pacific where island states are increasingly at risk from extreme weather events. With populations dispersed across scattered atolls, making contact with the rest of the world is challenging, especially during times of emergency. However, despite being classified as one of the most vulnerable global regions to climate change, Pacific countries are successfully building their resilience.Most Pacific island countries are developing Joint National Action Plans bridging climate change and disaster risk management, which includes approaches to support communities adapt to and reduce the impacts of climate change, while also addressing disaster risk reduction. A key element of disaster risk reduction is to ensure that information is continually available during times of emergencies and disasters. As a leading climate change adaptation organisation, the Secretariat of the Pacific Regional Environment Programme (SPREP), in collaboration with the Secretariat of the Pacific Community, has been focusing on strengthening national broadcasters' capacity to provide accurate and consistent information to island communities.One such initiative is the National Broadcast and Climate Disaster Resilience Plans (BCDRPs) involving broadcasters from eight Pacific countries (the Cook Islands, Palau and the Marshall Islands, Kiribati, Samoa, Solomon Islands, Tonga, Tuvalu and Vanuatu), selected and funded by the Pacific Media Assistance Scheme. Launched in 2012, the project's aim has been to support development of the BCDRPs as well as train broadcasters, help develop standard operating procedures, and provide capacity building to news teams for enhanced reporting on natural disasters and climate change.The most recent BCDRP training was held with the Tonga Broadcast Commission (TBC) in August 2015; as the government broadcaster, TBC -via Radio Tonga -is the only radio station which reaches all Tonga's islands. To help better understand terminology used in weather warnings and bulletins, TBC staff worked with the National Emergency Management Office and the Tongan Meteorological Service. As a result of the training, TBC was able to complete their BCDR plan, identify the resources needed in order to better respond to disasters, and keep the community informed. \"This is a very detailed plan, a first for TBC, which will help keep our people better educated during emergencies to ultimately save lives and properties,\" said Nanise Fiftia, TBC manager. Following completion of Samoa's national BCDRP in November 2014, a mock 'disaster' was simulated to test the plan's effectiveness and the roles of national media during an emergency. The event -an earthquake leading to a tsunami in Samoa -allowed broadcasters to practise who would give directions, what actions should be taken and how these would be done, as well as determine how vital broadcast equipment would be maintained during a disaster to keep information flowing to Samoan communities. Similar training and mock events have been held across the eight project countries and national BCDRPs are all in place. \"We are really pleased with the project outcomes on many levels,\" says Nanette Woonton, SPREP media and public relations officer. \"As of last year eight more Pacific island broadcasters across the region now have resilience plans, as a vital component of sharing information with the public in times of disasters, this is extremely important. Through this training we have also seen Pacific reporters come to understand technical meteorological and climate information and report with greater accuracy. We are now looking forward to continuing this work with media across more Pacific island states.\"The media is vital for raising awareness of climate change issues and sharing timely updates during emergencies, particularly for remote communities. An initiative is the Pacific is supporting broadcasters to provide accurate reporting prior to and during natural disaster events. With their small size, open economies, and reliance on natural resources, Caribbean states are particularly vulnerable to a changing and variable climate. In response, the region implemented two consecutive adaptation to climate change projects from 1997 until 2014, and following the success of these regional initiatives, Caribbean Heads of State called for a specific agency to coordinate subsequent activities. As a result, the Caribbean Community Climate Change Centre (CCCCC) was established as a centre of excellence in 2005 and is the world's only regional climate change centre.In its first decade, the centre has continued to pilot adaptation programmes, including climate data collection, climate projections, capacity building, and inclusion of climate change in national development processes. \"These actions have only been effective because the work has been grounded in firm regional commitment, policy and strategy,\" emphasises Dr Kenrick Leslie, CCCCC executive director.The work on regional climate change projections, for example, has been particularly vital for improved risk management. Through improved regional data collection and modelling, specific projections for areas as small as 8 km 2 are now available, compared with 300 km 2 previously provided by general circulation models. This has been achieved through extensive computer analysis conducted by a network of institutions and coordinated by the CCCCC.However, climate change projections alone are not sufficient and regional governments realised that building climate resilient economies would require transformational change at the institutional level. This challenge was recognised in the Liliendaal Declaration by Heads of State in 2009, in a regional framework to provide a roadmap for action from 2009 until 2015, and an implementation plan was further developed to deliver the framework's strategic elements.But, with member states and regional organisations already over-tasked and under-resourced, delivering such change requires an approach that recognises these constraints. To provide a solution, a sustainable resource mobilisation plan known as the 'Three Ones' principle, which was successfully used for a regional HIV/AIDS programme, was adopted. This is based on having one plan, one coordinating mechanism and one monitoring and evaluation framework, and works with a network of relevant organisations to utilise resources more effectively.Coordination, collaboration and partnerships have been key to the success of the CCCCC, and provide the foundations for the 'Three Ones' principle, helping to ensure a coordinated approach to climate change responses across Caribbean governments. Guided by this regional vision, the CCCCC has successfully executed a range of climate change-related programmes over the last 5 years, worth approximately €47 million. One of the tools developedThe Caribbean Community Climate Change Centre uses an innovative model to achieve the change required to respond to climate change and its impacts on development. Known as the 'Three Ones' principle, this involves one plan, one coordinating mechanism and one monitoring and evaluation framework for mobilising limited resources, policy setting and decision-making and monitoring.is the Caribbean Climate Online Risk and Adaptation Tool (CCORAL), which helps users to apply a risk management approach in decision-making and to prioritise their efforts given limited time and resources. The CCCCC and its partners are currently involved in extensive training and rollout of CCORAL across the Caribbean. \"Apart from some revisions, CCORAL has been widely accepted so far,\" says Keith Nichols, CCCCC project development specialist. \"We have seen some very useful applications in Grenada, including in agriculture, and interest has been high. We have not yet started full scale implementation but, to date, some national developmental activities have been revised to take into consideration climate risks. Rolling out has just started and the results are beginning to come in.\"\"Partnership is the way to success and without all our partners, we would not be in a position to do all the things we want to do,\" emphasises Kenrick Leslie. \"Through working together, climate change is now more widely covered in the region. Financing remains a challenge but for the future we have set up a trust fund to provide support in situations where external funds are not readily available.\"The CCCC work on climate change projections aims to help local governments to develop new climate adaptation policiesAlthough climate change affects everyone, young and old people, youth seem especially vulnerable because they will live longer and face the challenges throughout their lifetime. With reference to Africa, the situation is especially grim because Africa has the youngest population and agriculture is the sector that seems to offer the most opportunities for these millions of youth as they enter their working life. They do not see agriculture as a glamorous career option because they have seen how climate change disrupts agriculture. It makes it harder to make a case for youth to engage in agriculture. The youth see that agriculture is not profitable because it cannot guarantee an end return because of the varying weather patterns.How can climate change issues be tackled in a different way for the youth?Agricultural practices need to change. climate-smart agriculture, which looks at new and improved ways of producing food sustainably without damaging the environment and making sure crops that are being grown are resilient to the effect of climate change, is probably the answer for young people who want to engage in agriculture.What have been some key achievements with regard to youth to deal with the effects of climate change?FANRPAN mostly focuses on achievements in the policy arena, looking at how young people are now getting more involved in the climate change processes and how their voices are heard. The formal recognition of young people in the UNFCC process under the Youth NGOs constituency has been a major achievement.Young people can now make official statements and provide technical and policy input in climate change negotiations; they are now able to engage with policymakers through high-level meetings, seminars and thematic discussions. They have been involved in the process since 2009 and have made some progress. In terms of progress in Africa, the African Climate-Smart Agriculture Youth Group was formed in September 2014 during the Climate Summit in New York. This fairly new group wants to raise awareness, sensitise young people and build their capacity to implement climate-smart agriculture projects. And I think this is just the beginning.Considering the role played by women in gathering water and considering that by 2020, in some countries, yields from rain-fed agriculture could be reduced by 50%, what action needs to be taken towards young girls?There needs to be an increase in access to high quality education for girls, as much as for boys, as a start to addressing gender inequality issues. As long as young girls are not the priority in terms of schooling and education, we will still face the problem of them remaining at home to do chores, carry water and fetch wood. In addition, mainstreaming gender in climate change discussions is still finding its ground, a lot of work still needs to be done.What financing and policies will be required to enhance the youth's work on climate-related agricultural issues? Africa as a whole is still not getting enough money for climate financing, let alone the youth. The African Development Bank says Africa receives about €115 million compared to the €35 billion required.In terms of policies, we need clear guidelines and monitoring systems for climate change financing. And it will be important for African governments, once they get the money, to have structured climate-smart finance knowledge management systems where young people can have access to critical information. Also, sometimes information is just too complicated. This is another challenge, there is a need to simplify information and procedures around climate financing.The difficulty of engaging youth in agriculture Agriculture is not always very appealing to the youth and the impacts of climate change are making agriculture even less appealing as a livelihood. But by promoting new innovative practices and by giving youth a say in climate related matters, agriculture can be an attractive option.Sithembile Ndema Mwamakamba coordinates the Food, Agriculture and Natural Resources Policy Analysis (FANRPAN) youth and gender programme, aimed at developing a holistic agriculture policy framework in Africa to support youth and women.Have you noticed a change in the behaviour of farmers who have taken out index-based climate insurance?In terms of behaviour, the GIIF programme has undoubtedly introduced the insurance culture, consequently changing smallholders' view of insurance. For instance, tea growers in southcentral Sri Lanka who have taken out index-based weather insurance now readily discuss getting coverage for other non-climate risks such as snake bites, accidents, fire, and so on. People are now aware that these risks can be covered.In Kenya, initial studies showed that index-based natural risk insurance users had increased their investments in production tools, fertiliser and other inputs by 19% in comparison to nonusers. A 16% increase in their savings relative to others was also noted. These findings reflect a change in behaviour.Local insurers with a national operating licence are required for the development of index-based insurance. It is therefore essential that we develop their capacity to innovate, negotiate with international and regional reinsurers, and create a desire amongst producers for these new products.Forces that drive innovation come from both private and public sectors. Clearly public-private partnerships will be required in the medium-term to develop these markets and enable them to grow to a critical size through management and quality control of data and regulation. It is also essential to have a government that facilitates access to insurance by subsidising premiums and supporting education, especially under responsible insurance schemes. Governments have a key role to play at the outset, but also during the market growth phase.NGOs that serve as go-betweens with farmers?Yes. The entire ecosystem that develops around index-based insurance, especially weather-oriented insurance, includes governments, local insurers, reinsurers, distributors, NGOs and foundations. In Benin, Burkina Faso, Mali and Senegal, we are working with Planet Guarantee to develop farmers' interest in these products and to anchor indexbased insurance (especially climate insurance) in their communities. NGOs working in the microfinance sector play a major role because access to insurance is often linked with access to funding. These insurance schemes have often been set up with financial and technical assistance from donors and governments. Will they be commercially viable in the long run?We might have to introduce the 'subsidy' parameter in the viability concept. In India, 32 million farmers already use index-based insurance (based both on weather and yield). This would not have been possible without significant government support. Agricultural insurance schemes in developed countries also require subsidisation to progress, but that does not mean the system is unsustainable or non-viable. Setting a purely financial sustainability goal at the outset seems unrealistic. Ultimately, access to cheaper information, the development of a broader market, and the coverage of diversified risks at regional and global level will also enable reinsurance to be cheaper. This could ensure financial viability, which is what we are working towards.More than 800,000 crop and livestock farmers and microentrepreneurs have taken out index-based natural risk insurance through the Global Index Insurance Facility (GIIF), managed by the International Finance Corporation of the World Bank Group. GIIF was launched in 2009 to promote access to innovative insurance products in developing countries. Gilles Galludec feels that index-based insurance is the way forward.Gilles Galludec is the GIIF Programme manager with over 25 years' management experience in the banking, microfinance and insurance sectors, mainly in developing countries.In the context of climate change, the need to enhance food production while maintaining natural resources and the resilience of agro-ecosystems is increasingly important. Already, a number of land use and agricultural production practices exist that offer opportunities to both produce food and provide environmental services. However, adoption by farmers has generally been limited, not least because some of these practices only become profitable after 2-3 years or more. Thus, land users may have to absorb losses for several years before they can profit from their investment and for many, this is unaffordable. Payments for environmental (or ecosystem) services (PES) can offer a solution to this problem.PES are payments made to producers and landowners to reward them for managing their land in such a way that ecosystem services are generated, to the benefit of wider society. Such schemes aim at encouraging positive behavioural change of individuals and groups towards good environmental stewardship. They must, however, be very carefully designed and implemented to ensure they meet their objectives for the environment, provide appropriate economic incentives for farmers and land users and do not compromise food security. For example, farmers should not become exclusively 'carbon growers' as a result of a PES scheme.One significant challenge in implementing PES is choosing who to target. To be effective and affordable, PES needs to be carefully targeted and time bound, not a blanket incentive scheme for all land users. But the process of choosing who benefits must be transparent and fair, to minimise the risk of favouritism and tension within and between communities. A second issue concerns how much to pay. If too much is paid, the scheme will be too costly and not sustainable, whereas the risk of paying too little is that the scheme becomes exploitative and beneficiaries will drop out over time.In attempting to find the right level of payment, an approach using a \"reverse auction\" to reward on-farm tree planting has been implemented in Malawi and Indonesia. The approach offers an efficient way of designing appropriate rewards systems and helping to improve the overall cost-effectiveness of a PES scheme. Details of the approach have been documented in the World Development Journal (Ajayi et al. 2012).There is a need now to look beyond the narrow scope of cash payments, and consider other options, such as in-kind payments and other forms of targeted incentive. Such incentives could include offering conditional access to rural credit, or to niche markets that provide premium prices. Farmers could be offered access to subsidised farm inputs during the first few years of implementation, on the condition that they have adopted ecofriendly land use practices in their field.Beyond that, there is a need to identify and document the most promising land use practices, gathering clear, robust evidence on how they contribute to food production and address climate change, and how they can be potentially scaled up to more land users through various efforts, including PES, where necessary.A final point is that land users' decisions are strongly influenced by the policy and institutional context within which they operate. Appropriate policies at all levels are required to align smallholder farmers' incentives with the needs of society as a whole, and to encourage them to consider the environmental implications when making land use decisions. In this context, there is undoubtedly a need to appraise existing national and sub-regional policies to assess if and how they have inadvertently created incentives or disincentives to good land use practices.","tokenCount":"14634"} \ No newline at end of file diff --git a/data/part_6/0157256883.json b/data/part_6/0157256883.json new file mode 100644 index 0000000000000000000000000000000000000000..5687b5f414d0d6f8eacebe2bf79b76bf13ccbff5 --- /dev/null +++ b/data/part_6/0157256883.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"71b39e9e32d09a3316a57cd091b79e86","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/4e273929-b9b0-4d6e-b727-b1f8a17b4c17/retrieve","id":"240116939"},"keywords":["~~ k A¡\"","ü\"t:f•~.-\" T' ...... ~ ~;~ 4/'","1","l'•"],"sieverID":"93814cb9-3352-46b5-a035-1ff6788ede46","pagecount":"7","content":"MONOOMO (Cauca)\" \" , , \" l. Aspectos gene~a1es del departamentd.\"El departamento del Cauca se encuentra localizado en la región Sur-Occidental del País y su tel'ri\"torio\" estil atravesado por las Cordilleras Central y Occidental de 10s.Andes .. Cartográficamente se halla entre los 00°57' y 03\"20,'.' de Latitud Norte y entre los 75\"48' y 77° 57' de Longitud Oeste. Limita por el Norte con el Departamento del Valle del Cauca; por el Oriente con los departamentos de Nariño y la intendencia del Putumayo y por el Occidente con el departamento de Nariño y el Oc~ano Pacífico.El departamento tiene una extensión superficial de 30.495 Km 2 • de los cuales por la influencia orográfica un 34.5t aproximadamente pertenecen a un clima cálido, un 35.6% a un clima medio, un 19.7% a un clima frio y un 10.2% aun clima de páramo\" (1). Según un informe de OPSA. para el 'afio de 1977, en el país existían 218.330 Ha. en yuca de las '\" ~uales 17.000_(7.8%) se encontraban en el departamento del Cauea, con uñ reñdimiento promedio de 5.200 Kg/Hi. El pr~;;¡o-a ~i~~l na~ional ~~ -----es de 9.03~_~g¿Ha.Según esta misma fuente la producción de yuca es hecha en forma tradicional. en su totalidad y en forma de monocultivo.El censo Agropecuario realizado por el OANE en 1970-71 mostró que del total de las fincas visitadas en Santander de Quiliehao el 43.2% reportaban a la yuca como el cultivo principal.2. Características de los suelos de Mondomo .\".\"Originalmente esta unidad fué recubierta por una capa de ceniza volcánica de poco espesor y en la actualidad presenta como característica principal un avanzado estado de erosión, debido al cultivo en forma indiscriminada de la yuca, además de la presencia de vientos c&lidos que ascienden del .Río Cauca por las boyas de los FC~.La...J.e:L4.s-!Jl!U!!l!l!~~ y r~ondomo. AunqUE:! la precipitación es baja, cual ocasiona erosión severa. -2 -En general se presenta una topografía muy accidentada con pendientes. superiores al 40% y longitudes medias, aunque también hay pequeñas zonas de topografla más ondLiladas que corresponden a aquellas donde se ha conservado el recubrimiento de ceniza volcánica.En términos generales se observa. que son suelos de muy baja fertilidad natural. con alta presencia de aluminio, que sumado a las condiciones climáticas adversas son suelos de muy baja produ~tividad\" (1) 1.05 48.9 3. Características de la producci6n de yuca en Mondcimo gr. de suelo ppm = mg/kg de suelo ~egan los resultados del Censo agropecuario de las 504 explotaciones que reportan a la yuca como cultivo principal en .el municipio de Santander, el 54.9% tienen un tamaño inferior a 5 hectáreas de exten-si5n¡ el 21.2% se encuentran entre 5 y menos de 10 Ha. y el resto o sea el 23.9% son superiores a 10 hectáreas.En el mes de Marzo del presente año se aplicó una encuesta a 21 agricultores escogidos• al azar localizados en las veredas de El Turco. Mondomito. Nueva Colombia, todas pertenecientes al corregimiento de Mondomo.El tamaño promedio de las fincas fué de .11.7 Ha. Del total de agricultores entrevistados, el 95.2% tenían cultivos de yuca, con una -extensión promedio de 1.75 Ha; la altura promedio es de 1508 metros --s.n.m. el 95% eran propietarios y el resto arrendatarios.De la superficie total de las fincas encuestadas el 41.1% se destina--ba a agricultura; el 36.9% a 'cultivos permanentes y el resto o sea ,e~_~~.l% estaba,en cultivos transitorios. Un alto porcentaje de la 'tierra (54:6%) se encuentra en rastrojo o descanso; esto se explica a' la baja fertilidad del suelo y que los agricultores utilizan un sis-~tema rudimentario de control a la erosión: originada por el cultivo de -la yuca; es común sembrar 'en 'un lote 2,3 Y hasta 4 veces yuca para luego dejarlo en descanso durante 3-4 años; otros según la disponibilidad de tierra. 10 dejan hasta 10 años, por 10 tanto el agricultor se ve en'la obligación de tener gran parte dé la finca en uso improductivo.Solamente el 4.3% del área se encuentra en pastos. , 3.1. Utilización Agrícola del suelo .' .La' encuesta aplicada en la zona permitió conocer~l uso agrícola y los distintos cultivos que existen. Se describen én el cuadro siguiente: Cuadro 2. Uso Agrícola del suelo en la regi'on, de Mondomo (Cauca) (\"; .\" __ ... ,_. __ 0_.. .-~ -.,--' -~ .. _- De este cuadro se desprende que el 36.1% del área destinada a agricultura se encuentró, en el cultivo de yuca y que este cultivo cubre el 57.3% del área destinada a cultivos transitorios. La yuca presenta para el agricultor de la zona el 39.2% del valor total de la producci6n agrícola., \\ El cultivo de la yuca en la zona No existe una época determinada de siembra, la mayoría de los agricultores siembran en cualquier época del a~o; el cultivo se hace solo sin ningún otro cultivo i~tercalado. La preparación del suelo el 52% 10 hace con yunta de bueyes, el resto o sea el 48% hoyan el suelo manualmente; las variedades que existen en la región son = Algodona, Barranque~a. Americana y Vál1una. El períodovegetativo dfr las variedades Algodona, Barranque~a y American, es de 15 meses y para la Val luna es de 11 a 12 meses. Generalmente realizan tres desyerbas al cultivo: la primera a los 3 meses de sembrada, la segunda a 105 5 meses y la tercera a los 6 meses . Produce; ón de A lmi dón en 1 a zona Existen en ola zona 'de Mondomo aproximadamente 25 ra11andérías que fun--¿Tonan eñ-formao!leFrnaile,n'te durante todo el año, con algunas interrup-crones especialmente en las pequerias, las cuales tienen limitaciones -en el agua y la'cantidad de yuca a rallar. parece que las grandes no _ 1ienen problemas y pueden funcionar continuamente.A principios de este a~o CARE, SENA e lCA realizaron una encuesta a 14 ' o ,ralJa!lderías, localizadas en los municipios de Santander y Caldono.. ,-.~w. __ . ~~M\"_ ~.Los aspectos más importantes de esta encuesta se pr'esentan a continuación:2.Tas-raHan'dería's' compra-n 'yuca no solamente de la región, sino también en otros departamentos distantes tales como Huila y Putumayo. Los compradores de almidón de yuca localizados en Santander de Quilichao otorgan crédito a las rallanderías para que estos compren yuca a los cultivadores, asegurando de esta manera la compra de almidón • \" .. .:.~ ..-6cuente encontrar que estas rallanderfas compran yuca proveniente de otros departamentos.Extracción de almidón según variedades: Las R~llanderías por su experiencia alcanzada en muchos años de tra-'bajo, tienen su conocimiento sobre el contenido de almidón de las dls-,tintas variedades.La variedad que mejor precio tiene es la Barranqueña ($3.4 el kilo).. \"La algodona y AmericaQa tienen un precio de $2.85 el kilo. el almidón es comprado en Santander por los ~ntermediarios ti lo.Actualmente a $21.20 el En términos generales 175 kilos de yuca producen 12.5 kilos de afrecho y 525 kilos de yuca producen 12.5 kilos de mancha. Si la yuca es más añej a 1 a producci ón de mancha será mayo'r, yucas frescas producen me,nos mancha.La calidad del almidón está determinada por la cantidad de tiempo que se deje en el tanque asriador; en su totalidad el almidón que se produce en la zona es agrio.Generalmente el comprador de yuca puede guardar hasta 5 días el producto sin que se descomponga. Antes de ser rallado, sin cáscara solamente dura de 2 a 3 días. después se pudre.Organización y funcionamiento del Mercado del Almidón en la Zona , En el mercadeo del almidón se dan algunas características especiales qúe a continuación se describen:'En Santander de Quilichao se encuentran localizados cinco compradores de almidón (interme,diarios), los cuales funcionan como grupo oHgopólico al fijar semanalmente el precio del almidón que se va a comprar en la siguiente semana. Estos son los que distribuyen el almidón a las distintas industrias y panaderías que demandan el producto. En Mondomo existen 7 compradores de almidón, los cuales la mayor parte de almidón' la venden a los compradores de Santander de Quilichao. Algunos dueños de rallanderías para asegurar el suministro de yuca anticipan a los . \"• f ; ~ -7agricultores pequeñas cantidades de dinero ($500 -$1.000), dinero que ha sido prestado por los compradores de almid6n localizados en Santander, El preci o del a lmi d6n en Santander fl uctúa según 1 a cantidad de queso y el precio que éste tenga. Las panaderías son las principales consumidoras del almid6n y de queso. Al presentarse en época de verano una escasez de, leche y un aumento en l,os precios del queso, 1 a 'demanda por a lmi d6n di smi nuye. Las panaderías generalmente ,compran el almidón a un menor precio y lo almacenan hasta la aparición nuevamente de queso. 'El funcionamiento del mercado del almid6n de'yuca requiere un análisis más objetivo y profundo que será realizado en base a,esta primera aproximación ~l,conocimiento empírico y sus' particularidades que requieren ser conocidas con el fin de determinar el futuro que tiene el almid6n de yuca en el mercado nacional.(1) FEDERACION NACIONAL DE CAFETEROS DE COLOMBIA Estudio de zonificación y uso Potencial del suelo en la zona Cafetera del Departamento del Cauca. 1978 .","tokenCount":"1501"} \ No newline at end of file diff --git a/data/part_6/0159677497.json b/data/part_6/0159677497.json new file mode 100644 index 0000000000000000000000000000000000000000..9479765a62e8bce878b79cdae99ca43e9719380c --- /dev/null +++ b/data/part_6/0159677497.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"afc59fe6be68df5243c5e0b5a149070a","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/d571437c-1fd8-4f98-b877-15aae4533d8e/retrieve","id":"-2094961048"},"keywords":[],"sieverID":"3891c536-04e4-462e-945e-6528e4c6859f","pagecount":"11","content":"Food systems (FSs) emit ~ 20 GtCO 2 e/y (~ 35% of global greenhouse gas emissions). This level tends to raise given the expected increases in food demands, which may threaten global climate targets. Through a rapid assessment, evaluating 60+ scenarios based on existing low-emission and carbon sequestration practices, we estimate that intensifying FSs could reduce its emissions from 21.4 to − 2.0 GtCO 2 e/y and address increasing food demands without relying on carbon offsets (e.g., related to afforestation and reforestation programs). However, given historical trends and regional contexts, a more diverse portfolio of practices, including diet shifts and new-horizon technologies, will be needed to increase the feasibility of achieving net-zero FSs. One likely pathway consists of implementing practices that shift food production to the 30th-percentile of least emission-intensive FSs (~ 45% emissions reduction), sequester carbon at 50% of its potential (~ 5 GtCO 2 e/y) and adopt diet shifts and new-horizon technologies (~ 6 GtCO 2 e/y). For a successful transition to happen, the global FSs would, in the next decade (2020s), need to implement cost-effective mitigation practices and technologies, supported by improvements in countries' governance and technical assistance, innovative financial mechanisms and research focused on making affordable technologies in the following two decades (2030-2050). This work provides options and a vision to guide global FSs to achieving net-zero by 2050.The Paris Agreement's goal of limiting the increase in global temperature to 1.5° above pre-industrial levels requires rapid and ambitious reductions in global greenhouse gas (GHG) emissions. This can only be achieved by drastic emissions reductions across the energy; industry; transport; buildings; and agriculture, and forestry sectors 1,2 .Even if fossil fuel emissions stopped now, current trends in global food systems (FSs) would prevent the achievement of the 1.5 °C target and threaten the achievement of the 2 °C target by the end of the century 3 . However, carbon budgets or net-zero emissions are often only discussed for CO 2 emissions and not for non-CO 2 emissions, such as CH 4 and N 2 O, in which FSs, especially agriculture production, are the main source [3][4][5] .Today, FSs GHG emissions contribute to roughly a third of global emissions. In 2019, FSs emitted 16.5 (95%; CI range: 11-22) GtCO 2 e globally, the largest contributors were agriculture, land use, land-use change activities (~ 70%) and the remaining emissions coming from other downstream and upstream activities (i.e., retail, transport, consumption, fuel production, waste management, industrial processes and packaging) 6 . Since global food production is estimated to increase by 15% in coming decades 7 , FSs emissions might increase by up to 80% from 2010 to 2050 3,6,[8][9][10][11] . In addition, there are still almost 700 million people undernourished and living under severe food insecurity 12 who must be considered in FS planning. Therefore, the Paris Agreement and Sustainable Development Goals can only be achieved with significant contributions from FS, including supply-side measures in agriculture production and demand-side measures related to diet changes and reduced food waste 5,13 , while strengthening food security and safety 14 .Substantial GHG emissions reductions in FSs are attainable by implementing low-emission interventions to improve efficiency and nature-based carbon sequestration 3,5,15 . Low-emission interventions could result in ~ 40-70% less GHG intensive production systems compared to today's average levels 16 . Additionally, a carbonwww.nature.com/scientificreports/ sequestration potential, of approximately 10 GtCO 2 y -1 , is associated with FSs production under the expansion of agroforestry systems, improved pasture and crop management and application of biochar to soils 5 .Nevertheless, the mitigation benefits of improved systems could be offset under food production's current growth trajectory, especially for livestock production 10 . Even with higher efficiency, greater production needed to meet growing demand might increase net GHG emissions. This condition suggests that dietary changes, including a reduction in consumption of livestock products and replacement by plant-based foods, is also important to help transition to low-carbon and net-zero food systems 5,8,13,15 . Furthermore, several technologies developed or under development might help further reduce emissions in the medium and long run, such as feed additives for livestock, novel perennials, soil additives, nanoproducts and intelligent food packaging 17 .Therefore, a combination of actions (e.g., implementation of low-emissions interventions for improving production systems efficiency, promotion of carbon sequestration; reduction in livestock-based protein consumption and deployment of new-horizon technologies) is likely necessary to reduce net GHG emissions of FSs aligned with net-zero emissions strategies 10,15 .Although the impressive commitment to the net-zero agenda of countries and the world's biggest food companies, guidance offering multiple options for achieving net-zero emissions in global FSs and informing the effectiveness of pledges and catalyze meaningful climate action is still needed. To date, most studies have focused on estimating global food systems emissions 6,18 and evaluating potential mitigation through a few and aggregated pathways using complex models 3,10 and none has proposed a roadmap towards net-zero food systems, which has lately been highly demanded by several food systems actors 19 .Through a rapid assessment using three datasets, the FAO forecast on global food production by 2050 7 and food value-chain emissions intensities 16 and carbon sequestration potentials 5 , we built 60+ pathways towards 2050 by analyzing global food demands with the implementation of four major interventions in FSs: (1) implementing low-emission practices to reduce emissions through increased production efficiency (10th, 20th, 30th, 40th pctl of least emission-intensive systems and average); (2) sequestering carbon in croplands and grasslands; (3) shifting diets to reduce global production of livestock-based protein; and (4) adopting new-horizon technologies across food value-chains. In calculating these contributions, we also provide a vision, with examples, to downscale global sectoral goals to the regional level, highlighting areas where improvements are needed. It is important to note that since our analysis is limited to a global overview, the implications of FSs intensification may have different consequences at regional scales. Further analysis is needed to shed more light on the possibility to mix different intensification strategies to optimally meet socio-economic and environmental targets. However, as countries and companies begin implementing their pledges and establish sectoral targets, our analysis provides a transparent, scientific basis for gauging the ambition of these contributions to global net-zero food systems.Food system emissions snapshot. We estimate that global FSs emitted 18.7-21.4 Gt CO 2 e/y from 2010 to 2020 (Fig. 1). This estimate is consistent with the emissions range of recent estimates covering the same period (9-22 GtCO 2 e/y) 3,6,16,18,[20][21][22] . Four value-chains-beef, milk, rice and maize-are responsible for nearly 65% (13.9 GtCO 2 e) of total FS emissions, and seven value-chains (+ wheat, pig and poultry) are responsible for almost 80% of emissions (17.2 GtCO 2 e). Livestock production (meat and milk) alone accounts for 60% of total FSs emissions (12.6 GtCO 2 e) (Fig. 1). Close to 70% of FS emissions come from land-use change and farming activities 6,16 .The production of major grains, meat and milk is projected to increase 29-81% by 2050 compared to today's levels 7 . Under current average production practices, meeting the 2050 projected food production 7 would increase FS emissions by 38% (~ 8 GtCO 2 e/y) compared to 2020, respectively (Fig. 1). These findings are consistent with recent analyses that have suggested that global FS emissions might increase 30-50% by 2050 11 .We find that the adoption of low-emission practices could shift global FSs production from the average to the 40th, 30th, 20th and 10th-percentile (pctl) of least emission-intensive systems 16 and could reduce the emission of 9.1-13.2 GtCO 2 e/y in 2050 compared to the 2020 base year level (21.4 GtCO 2 e) (Fig. 2). Major contributions would come from livestock and rice value-chains (Fig. 2).Although these FS value-chains are the most emission-intensive ones, they also have the largest mitigation potential across FSs (Fig. 2). For example, improving production practices with existing technologies could reduce emissions by 40%-70% compared to average values: beef from 7.3 to ~ 2.5 GtCO 2 e/y and rice and milk from 2.4 to ~ 1.0 GtCO 2 e/y (Fig. 2). Most of this mitigation potential is related to reductions in land-use change (e.g., deforestation for agricultural land expansion), improvements in animal feeding and breeding and manure management, nutrient management (with focus on nitrogen fertilizers), water management in rice paddies and energy efficiency (e.g., renewables) across the value-chain as well as measures to reduce food loss and waste (i.e., improved packaging and storage) 5,16,17,23,24 . Also, using a global warming potential accounting for short-lived GHGs (GWP*), like CH 4 , means that relatively small annual reductions in CH 4 emissions (~ 0.3%) could eliminate global warming caused by the emissions of CH 4 from biogenic sources in 20 years [25][26][27] .Harnessing the carbon sequestration potential associated with low-emissions agricultural practices could contribute to an additional emission abatement of 10.5 GtCO 2 e/y 5 . Most of this potential is related to the belowand above-ground carbon accumulation with the expansion of agroforestry systems (5.6 GtCO 2 e/y) and soil carbon sequestration with improvements of pasture and crop management (2.5 GtCO 2 e/y), such as the adoption of reduced and no-tillage and grass-legume mixtures in pastures, and the application of biochar to soils (2.4 GtCO 2 e/y) 5 . Furthermore, it is worth noting that these mitigation actions also have synergies with food productivity, climate adaptation, and other environmental aspects (e.g., water and soil conservation) 17,28,29 .www.nature.com/scientificreports/ Reduction in livestock-based protein consumption. Reducing livestock-based protein consumption is often pointed out as another option to reduce GHG emissions from food systems 3,8 . Nevertheless, under current average livestock production practices, a reduction of livestock-based protein consumption would only decrease livestock emissions in 2050, compared to the 2020 levels, if projected production is cut over 25% (Table 1). At or below this level, livestock emissions would rise or be kept constant considering today's average production system emissions and projected increases in meat (+ 37%) and milk (+ 29%) productions by 2050 7 (Table 2). On the other hand, if accompanied by the implementation of low emission practices, reducing the consumption of livestock-based protein by 10% and 25%, for example, could promote emission reductions of 0.5-2.5 GtCO 2 e/y by 2050 (Table 1). Therefore, scaling the implementation of low-emissions practices to improve livestock production is a precondition to drive significant changes in emissions towards net-zero FSs.New-horizon technologies. New technologies to reduce GHG emissions from FSs include those that are still costly (Roe et al. 5 ) and primarily not yet present in food value-chains but could increase mitigation from GHG-efficient food production practices, land-use change, and carbon sinks 30 . This diverse pipeline, including consumer-ready artificial meat, methane inhibitors, intelligent packaging, vertical agriculture, nano-drones and 3-D printing, presents real opportunities for systemic change 17 . Also, if these technologies are developed to reduce costs of existing agricultural-related practices that are not cost-effective today (e.g., > 100 USD/tCO 2 e), it could unlock emissions reductions and carbon sequestration of approximately 8. 5 to 40% of today's FSs emissions and 50% of agricultural-related mitigation potential 5 . For example, the implementation of agroforestry has the technical potential to sequester approximately 11.2 GtCO2e/y, but only 20% of this potential is considered cost-effective today 5 .Food system mitigation potential. By randomly combining the implementation of major FS mitigation actions to target net-zero emissions by 2050 in 64 scenarios, we found that only eight would lead to net-zero FSs through the implementation of existing low emission and carbon sequestration production practices (Fig. 3), another eight scenarios would need to further rely on diets shifts and the remaining 48 would need additional emission reduction with the implementation of new-horizon technologies reducing up to 5 GtCO 2 e/y (Fig. 3). Through the implementation of existing low-emission and carbon sequestration practices only (i.e., excluding the reduction in livestock-based protein consumption and new-horizon technologies), we estimate that FSs emissions could shift from 21.4 to ~ − 2.0 GtCO 2 e/y by 2050 (i.e., 110% reduction compared to 2020 level by moving FS to the 10th pctl of least emission-intensive practices and harnessing 100% of the carbon sequestration potential) (Fig. 3).The higher the implementation of low-emissions practices (i.e., towards the 10th pctl of leasts emissionintensive systems), the lower the dependance on carbon sequestration, reduction of livestock-based protein consumption and new-horizon technologies. Therefore, scaling low-emissions practices to improve FSs production is fundamental to feasibly driving significant changes in emissions towards net-zero FSs (Fig. 3). The conditions for harnessing the full FSs mitigation potential in the next three decades are ambitious given the cost-effectiveness of practices, differences in regional contexts (e.g., cost of implementation, institutional and technical capacity, and food access and demands), historical trends and uncertainties related to carbon sequestration 5,11,13,31,32 . For example, over the last 30 years (1988-2017), global productivity of cereals, rice, beef and dairy increased 9-40% while emission intensity (at farm level-major emission source; Fig. 2) was reduced by 7-40%, respectively (FAO-Stat, 2021). These numbers are far behind the emission reduction potential of ~ 65% (i.e., 10th pctl least emission-intensive systems) and more compatible with the 40th pctl least emission-intensive systems (Fig. 2). Only about 50% of the technical mitigation potential of existing agricultural-related practices and technologies are cost-effective today (e.g., up to 100 USD/tCO 2 e), and close to 75% of that is in developing (~ 65%) and least developed (~ 10%) countries (Roe et al. 5 ). This may add extra financial, technical and policy constraints for implementing FSs net-zero emissions plans, as developing and least developed nations likely have lower institutional capacity for implementing more effective climate policies 33 .There are still concerns regarding carbon sequestration permanence, which encompasses issues related to the time and vulnerability of the carbon sequestered in soils and biomass, such as (i) differential sequestration rates over time and long run decline to a near-zero rate, and (ii) release of sequestered carbon back into the atmosphere after discontinued carbon sequestering practices 31,32,34 . These aspects suggest that bolder actions to mitigate GHG from FSs are necessary to increase chances to achieve net-zero FSs emissions by 2050; according to the strategies and assumptions evaluated in this work, there is no silver bullet, and a combination of actions should therefore be targeted to increase the feasibility of achieving net-zero emission FSs by 2050 (Fig. 3).The roadmap for net-zero food systems. Without relying on carbon offsets (e.g., related to afforestation and reforestation), FSs have the potential to reach net-zero emissions by 2050 (Fig. 3), but countries' contextual constraints are likely to limit the potential reach of implementation. However, recent engagement of global FSs actors, along with advances in the plant-based protein industry and disruptive technologies 17,35,36 , has created momentum for action that may speed the implementation of low-emission and carbon sequestration practices, as well as the dissemination of diet shifts, to move FS emissions away from current trends. In this context, a vision for a net-zero FSs encompasses:• Large-scale adoption of low-emission practices to shift the production to the 30th pctl of least emissionintensive systems (~ 45% emissions reduction across FSs), which could mitigate 10.6 GtCO 2 e/y, or ~ 50% of the mitigation needed by 2050 compared to the 2020 base year. • Realizing 50% of the carbon sequestration potential associated with low-emission practices (i.e., soil carbon, agroforestry and biochar) could contribute another ~ 24% (5.2 GtCO 2 e/y) emission reduction. • Reducing the remaining FS emissions (5.6 GtCO 2 e/y) by decreasing 2050 projected livestock production, especially in high-and middle-income countries, in 25% (1.2GtCO 2 e/y) and by deploying new-horizon technologies (4.4 GtCO 2 e/y) (Fig. 3).Major actions to implement this vision over the next three decades could be summarized as follows:• By 2030, implement cost-effective actions to reduce CO 2 emissions from land-use change (e.g., deforestation and other land conversion) for food production along with using existing technologies to improve (i) beef, milk and rice production and (ii) nutrient management (focusing on nitrogen fertilizer) across major grain production systems (e.g., maize and wheat). By 2040, low-emissions agricultural practices should be implemented to harness the remaining cost-effective mitigation potential. Of this mitigation potential, 55% to 87% could be achieved with practices costing up to 100 US$/tCO 2 (Fig. 4; Table 2). • Implement cost-effective technologies and practices to sequester 1.7, 3.5 and 5.2 GtCO 2 annually by 2030, 2040 and 2050, respectively. This can be achieved by adopting agroforestry, applying biochar to soils and improving crop (e.g., tillage and cover crops) and pasture management (e.g., rotational grazing and fertilization) practices. Close to 45% of the carbon sequestration potential (4.8 GtCO 2 y −1 ) would cost up to 100 US$/ tCO 2 (Fig. 4; Table 2). • By 2040, scale the use of renewable energy (e.g., wind and solar), enhance fuel efficiency, expand the electric transportation fleet, improve fertilizer production, expand the circular economy and peri-urban agriculture, and promote diet shifts in high-and middle-income countries (Fig. 4; Table 2). • From 2040 to 2050, develop and produce affordable new-horizon technologies for negative emissions, with focus on livestock production systems (e.g., methane capture, feed additives and new breeds), novel plants and perennials for carbon sequestration and enhanced energy efficiency for storing, processing, transporting, packaging and retailing. Approximately 5.6 GtCO 2 e/y (2.6 and 5.7 GtCO 2 e emissions reduction and carbon sequestration, respectively)-or ~ 25% of the mitigation needed for net-zero FS-could be unleashed with the reduction of implementation costs (today above 100 US$/tCO 2 ) (Fig. 4; Table 2).Making net-zero food systems realistic. Our results show that the implementation of major mitigation actions for intensifying FSs based on existing low emission and carbon sequestration practices have the potential to reduce FSs emissions beyond net-zero by 2050 while increasing food production. Our analysis also demonstrates that an intensification strategy with a more diverse portfolio of practices, most notably diet shifts and new-horizon technologies, will be more effective for reaching net-zero emissions by 2050 without relying on carbon offsets (e.g., related to afforestation and reforestation).Even so, this scenario may not be realistic under today's trends considering that net-zero FSs require reducing emissions by 3.3% or ~ 700 MtCO 2 e annually between 2020 and 2050. In 2020, global fossil fuel emissions dropped 5.4% as a consequence of the COVID-19 pandemic, which is an unprecedented emissions reduction (at least since 1970) 37 . However, as the global economy is rebuilt, a rebound of 4.8% is expected in 2021 37 , leaving a net emissions reduction of just 0.6%. These numbers illustrate how difficult and massive the challenge to change current production patterns and reduce emissions is. This scenario could be different for FSs given the recent engagement of global FSs actors with the climate agenda and climate commitments (e.g., UNFSSS, Global Methane Pledge, and SBTi) 38 . Along with significant advances in the plant-based protein industry and disruptive technologies, this engagement has created a momentum for action that may speed up the implementation of steps to move FSs emissions away from business-asusual trends.Against this backdrop, implementing cost-effective measures and making affordable practices and new-horizon technologies in the coming decades seems to be a reasonable mitigation pathway for increasing the chances of food systems achieving net-zero emissions by 2050. To make net-zero FSs realistic it is essential to overcoming barriers, for example, related to regional contexts (e.g., cost of implementation, institutional and technical capacity, and food access and demands), historical trends, and uncertainties related to carbon sequestration 5,11,13,31,32 . Furthermore, to realize ambitious emissions reductions, FSs actors must coordinate and promote improvements on several other fronts, including institutional capacity (i.e., governance), finance, research, and technical assistance, especially in developing and least developed countries, and plan major emission reductions in the short run using current cost-effective practices. This would improve the feasibility of net-zero commitments and make FSs less dependent on the success and affordability of new-horizon technologies for large-scale negative emissions (which are uncertain at the moment) and cause carbon-intensive industries to stop growth and move to less intensive options.The mitigation potential of FSs interventions must also be validated against efficacy and cost-effectiveness across regions to avoid unintended consequences and minimize trade-offs 39,40 , which safeguards the effectiveness of practices in reducing emissions and enhancing food production and security. To support this process, research could be directed to tailor practices for different contexts, while making affordable new-horizon technologies in the medium-and long-term. This process must be done in close coordination with technical assistance for effective adaptation and implementation of mitigation and carbon sequestration practices on the ground along with farmers, in conjunction with assistance to meet monitoring, reporting and verification (MRV) of emissions requirements 41 . Science-based targets (FLAG) could be a reference as well as carbon market standards (e.g., VERRA and Gold Standard). Global benchmarks 11 must also be kept up to date to track the implementation of food system actions and commitments.Critically, the reorientation of both public and private sector sources of capital is needed to achieve net-zero emissions in global food systems by 2050. Firstly, financial mechanisms supporting the adoption of practices to realizing net-zero could be created by orienting traditional bank loans for positive climate impact, and scaling other approaches, such as blended finance and carbon markets 42,43 . Traditional bank loans offer a pathway to scale validated cost-effective technologies given the position of the lender to incentivize technology adoption. However, following the experience in the sector of renewable energy and energy efficiency, this requires access to patient capital and technical assistance for building the capacity of financial intermediaries, especially in developing and least developed countries, to construct loan portfolios and design incentive mechanisms that are explicitly linked to climate outcomes (e.g., Global Climate Partnership Fund-GCPF). The public sector can support in developing institutional frameworks such as cost-effective assessment and monitoring frameworks to enable the growth of such portfolios.Secondly, inovative financial mechanisms are needed to demonstrate the viability of investments in the adoption of low-emission interventions and carbon sequestration practices in developing and least developed countries, as well as absorb some of the early risk and up-front cost associated with a shift away from business as usual. Strategically allocating public sector capital to de-risk some of the private sector challenges (i.e. blended finance mechanisms etc.) and incentivizing the private sector to create new investment opportunities (i.e. carbon markets etc.,) are critical transition tools to build a diversified portfolio of cost-effective technologies. Furthermore, overlaying and co-designing such mechanisms with large corporations through, for example, implementing customized and collaborative corporate insetting programs within shared supply chains can ensure buy-in while contributing to the net-zero transition.Lastly, new funding models are required to sustain inflows of high-risk capital to incubate and accelerate new horizon technologies, especially to move technologies from the investment readiness phase to the implementation phase. Public sector can support in creating an enabling environment for such programs, especially in developing and least developing countries where models are less developed.Evidence shows that countries with better governance have more effective climate policies and could help maintain the integrity of the net-zero target while avoiding unintended consequences due to policy changes 44,45 . Investing in education, especially in regard to gender, is a key predictor of higher levels of governance. Increasing societal awareness of the need to support changes in food systems and consumption patterns is also fundamental for driving transformational change 14 .To foster this scenario at a global level, FSs net-zero plans could put more emphasis in the short run on a strong coalition of developing and developed nations, which are likely to have a higher capacity, while build capacity in developing and least developed countries, where international cooperation may also help.Since our analysis is limited to a global overview, the implications of FSs intensification may have different consequences at regional and country scales. Therefore, it is important that further analysis shed more light on the possibility to mix different intensification strategies to optimally meet socio-economic and environmental targets. Furthermore, data validation (e.g., emission factors and food production) is key for refining findings as well as recommendations for food systems stakeholders. This is especially applied to the levels of emission and emissions reductions while enhancing food production efficiency 16 , as well as carbon sequestration in agriculturebased systems 5 .Although net-zero FSs are achievable, bolder implementation of more efficient production practices is fundamental to feasibly meet both global food production and climate goals. This work provides an overview of this challenge along with a vision that could guide FSs actors towards these objectives.To estimate current and future FSs emissions and design strategies to achieve net-zero emissions by 2050, we evaluated emissions from 19 major crop and livestock (food) value chains by multiplying their respective global domestic production projections under business-as-usual 7 by a range of value-chain emissions intensities (10th, 20th, 30th, 40th pctl of least emission-intensive systems and average) 16 (SM). This approach permits to estimate total food value-chain emissions at different emission intensities (percentiles) that can be further used to evaluate potential changes in emissions by shifting production system efficiency. Although there has been a business-as-usual increase in food production efficiency, this rapid assessment assumed that the business-asusual FS GHG emissions per unit of food produced remain constant at current levels-although we further discuss business-as-usual trends in the main text. For livestock value-chain emissions, we deducted emissions from feed production 16 to avoid double-counting the emissions from the production of feed ingredients (e.g., grains). Emissions intensities 16 encompass the emissions of major GHGs released through FS operations from \"farm to fork\": carbon dioxide (CO 2 ), nitrous oxide (N 2 O), and methane (CH 4 ).We estimated changes in FS emissions starting in 2020 through 2050 for multiple scenarios:• Implementation of low-emissions interventions to shift production to the 40th, 30th, 20th and 10th pctl of least emission-intensive systems 16 . Lower percentiles are associated with no or reduced land-use change and food loss and waste 15 . • We considered that this shift would promote carbon sequestration in cropland and grassland soils (through best management practices), above and below-ground agroforestry systems and the application of biochar to soils 5 . We tested the realization of those potentials at 50, 75 and 100% 5 . We did not consider the carbon sequestration potential from afforestation and reforestation (A/R) and other natural ecosystem restoration (e.g., mangroves and peatlands) to FS 5 . We also assumed the eventual spared area used for feed production would be directed to expansion of other crops for human consumption. • Reduce global production, driven by lower consumption, of livestock-based protein (meat and milk) by 10, 25 and 50%, calculated using the 2050-projected levels as reference 7 . We assumed that reducing consumption of livestock products lowers milk and meat production. This process should slow demand growth, and eventually reduce the number of livestock heads-the major GHG source in the agricultural sector.• Adoption of new-horizon technologies across the food value-chains. These technologies include those that are not yet present on farms but could increase mitigation from GHG-efficient food production practices, land-use change, and carbon sinks 30 , as well as make current cost-ineffective practices and technologies affordable 5 .We built a pathway towards 2050 by assuming these strategies would be implemented at a rate of 20, 50 and 100% by 2030, 2040 and 2050, respectively. For livestock and rice production, we adjusted 16 data to reflect the contribution of CH 4 emissions to warming potential using the GWP* concept [25][26][27] . Under GWP*, stable CH 4 emission rates contribute a relatively small CO 2 e emission. Increasing CH 4 emission rates are reflected as a large CO 2 e emission and can exceed the GWP -100 of CH 4 if rates increase at more than approximately 1% per year. Declining CH 4 emission rates are reported as a negative CO 2 e emission and can reach zero CO 2 e if emission rates decline by 0.35% per year over 20 years. For that, we consider 2020 as the base year where the GWP* concept was applied. We must also consider that approximately 70% of the emissions from livestock and rice production are in the form of CH 4 11 and that approximately 70% of these emissions come from farm level 16 . Despite providing 60+ pathways for achieving net-zero FS using a transparent and accessible methodology and framework, certain limitations and gaps remain, especially on data sources related to the FS emissions factors and carbon sequestration potentials used in this work. For example, the development of FS emission factors percentiles relied on several studies evaluating emissions across a number of food value-chains 16 . As some of those studies reported group farms into a single observation and/or provided an impact average and its associated standard deviation, to include intrinsic sources of variance across parts of the value-chain and across observations (e.g., emissions factors, processing, packaging, retail, and transport impacts; processing conversions; and other conversions), the authors re-specified all values associated with variance as normally distributed variables. As pointed out by the authors, this approach may have limitations if studies are not reporting standard deviations or if they are remodeling from inventory data were used to fill different emissions gaps for each study. Nevertheless, the approach was likely one of the best way to incorporate multiple sources of variance found across studies to develop emissions percentiles. Similar limitations may also apply to the carbon sequestration dataset used in this work 5 , it also relies primarily on several previous research to derive carbon sequestration potentials. However, by updating global and regional mitigation potentials using both sectoral and integrated assessment model (IAM) approaches and comparing the results of both approaches, this study significantly improved the estimation of land-based mitigation potentials. Additional research is however needed for validating key datasets for estimating emissions and removals in FS across difference geographies and contexts and, ultimately, refining recommendations for FS stakeholders. This is especially applied to the attainable levels of emission and emissions reductions while enhancing food production efficiency 16 , as well as carbon sequestration in agriculture-based systems 5 (Supplementary Information).","tokenCount":"4984"} \ No newline at end of file diff --git a/data/part_6/0193068463.json b/data/part_6/0193068463.json new file mode 100644 index 0000000000000000000000000000000000000000..25adc818d71e2bcd81327fc983bcb79f930ec405 --- /dev/null +++ b/data/part_6/0193068463.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"e5857a86d809d50e771973634bf46e73","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/2a0df74e-dcc2-4c42-b7af-ebd7a729b126/retrieve","id":"-70940363"},"keywords":["Cassava","Stictococcus vayssierei","altitude","screening","tolerance Manioc","S. vayssierei","altitude","criblage","tolérance Variétés (Noms et/ou Code) Couleur et goût de Pulpe Maturité Comportement face aux maladies et ravageurs Rendement (T/Ha) Origine Butamu/Mv99/0395 Jaune","doux (Sucré) 12 mois Résistance à : Mosaïque","Bactériose","Acarien vert 20-25 INERA Disanka/I96/0211 Blanche (Amer) 12 mois Résistance à : Mosaïque","Bactériose","Acarien vert 30-35 Mvuazi/I95/0528 Blanche","doux (Sucré) 12 mois Résistance à : Mosaïque","Bactériose","Tolérance à : Acarien vert 35-40 Nsansi/I95/0160 Blanche","doux (Sucré) 12 mois Résistance à : Mosaïque","Bactériose","Susceptible à : Acarien vert 35-40 IITA-IBADAN Sawasawa/MM96/3920 Blanche","doux (Sucré) 12 mois Résistance à : Mosaïque","Bactériose","Tolérance à : Acarien vert"],"sieverID":"26a96794-78dc-4b93-8ba1-1f41b4db89e3","pagecount":"17","content":"Cassava is, economically, one of the most important root and tuber crops in Africa. However, its production is compromised by a large number of diseases and pests among which the african root and tuber scale (Stictococcus vayssierei Richard). This insect causes damage that can result in a low yield and non tuber formation of cassava in case of serious infestation. It is in this context that a study on screening cassava (Manihot esculenta CRANTZ) genotypes was carried out for resistance to African root and tuber scale in different agro ecological areas of Beni (North Kivu province, in East of the Democratic Republic of Congo). The objective of the study is to assess resistance to African root and tuber scale of 40 varieties of cassava supplied by the International Institute of Tropical Agriculture and the National Institut of Research Agriculture whose five local are found on the spot after survey. To reach this goal, we verified the hypothesis that stipulates that the introduction of new varieties of cassava in the country is a strategy to fight African root and tuber scale. All varieties were placed in four sites under mild altitudes (1000-1200 m) and high altitudes (1200-1400 m). The populations of African root and tuber scale in various stages of life and the tuberous root production of each variety were assessed every three months for two years in a randomized block experimental with four repetitions. The screening ANOVA findings showed significant effects, S. vayssierei infested the majority of tested varieties. Nevertheless, principal components analysis (PCA) and cluster analysis identified a reconciliation of tolerance vis-à-vis African root and scale in some improved varieties while released viz, Liyayi (MM96/0287), Obama (TME 419), Mvuazi (I95/528), Dinsaka (I96/0211) and while developing such as 20BI, 20B2, 20B4, 20B16, 20B27, 20B28, 20B29, 81, MM96/0105, MM96/4653, MM96/5272, MM96/5475, MM97/2206 as well as on some local varieties such as MUKAKASA, MBAYILO in different agro ecological areas of Beni Territory in view of the marketable tuberous root production at the end of screening. All the above-mentioned varieties gave a yielded ranging from 20, 3 T/ha to 74, 96 T/ha, included or over the interval acceptable by officially released varieties in DRC. The results indicated that the introduction of improved cassava varieties could be one of the alternatives for African root and tuber scale integrated management. Also, they show the importance of local varieties research for the development of cassava subsector.Le potentiel productif du manioc en Afrique est gravement compromis par la cochenille africaine de racines et tubercules (Stictococcus vayssierei Richard). En effet, en République Démocratique du Congo (RDC) le manioc (Manihot esculenta Crantz) est la principale culture vivrière et occupe 50% des superficies de toutes les étendues sous cultures vivrières [1].Les cochenilles extraient la sève des plantes, endommagent les tissus végétaux et injectent des toxines ou des virus chez nombreuses plantes d'importance économique [2]. De plus, les trous laissés sur les tissus végétaux constituent une porte d'entrée pour de nombreux pathogènes. C'est le cas de Stictococcus vayssierei Richard [3], [4], [5] ou la cochenille africaine de racines et tubercules (CART). Sa présence a été signalée pour la première fois en RDC dans le District du Bas-Fleuve, province du Bas-Congo au cours des années 1970 [6].C'est vers la fin de l'année 1980 que cette cochenille est devenue un ravageur nuisible majeur dans les champs de manioc dans certains pays tel que le Cameroun [7]. Le statut de ravageur de cette cochenille serait tributaire de l'intensification de la culture du manioc qui a entraîné le changement dans les pratiques culturales du milieu [8] [9]. La CART infeste les parties souterraines du manioc sur lesquelles elle se nourrit pendant tout son cycle de vie [10]. Les dégâts de cette cochenille sur le manioc entraînent le manque de la tubérisation ou le faible rendement en cas d'infestation sévère [11]. À cause de l'importance alimentaire et économique du manioc, il nous semble plus raisonnable de penser à augmenter le rendement tout en réduisant les pertes par des moyens qui n'entraînent que peu ou pas d'effets indésirables sur l'environnement.Face à la résistance des cochenilles aux produits chimiques [12] et à la dégradation de l'environnement par ces derniers, la lutte intégrée passe actuellement comme la meilleure alternative. Cette lutte nécessite entre autre la maîtrise de l'écologie du ravageur et du potentiel génétique du matériel végétal, notamment la résistance aux maladies et ravageurs dans les agrosystèmes [13]. La rareté des données se référant à la résistance de variétés de manioc aux infestations de la cochenille S. vayssierei mérite une attention particulière. Au stade actuel aucune recherche n'a été menée à l'Est de la RDC, particulièrement dans le Territoire de Beni où des infestations énormes des cochenilles radicoles sont observées même en haute altitude non forestière alors que selon [3], les cochenilles ont été décrites comme étant endémiques dans les zones forestières humides de basses altitudes. Le choix de ce sujet se justifie par le fait que le manioc en Afrique joue deux rôles majeurs pour les populations : celui de culture de sécurité et celui de production de base pour le développement économique des régions pauvres [14]. Ses racines tubéreuses sont des matières premières des industries d'amidonneries et ses feuilles sont riches en vitamines et sels minéraux et constituent le principal légume et même le principal complément alimentaire de base de nombreux Congolais [15]. L'examen de la relation causale entre les effets dévastateurs de la cochenille africaine de racines et tubercules et la productivité du manioc nous a inspiré deux idées fortes en guise d'hypothèses :• Dans le germoplasme actuellement disponible en RDC, il y a des variétés de manioc résistantes à la CART.• Le test du germoplasme constitué de plusieurs génotypes permet l'identification des sources de résistance de manioc à la cochenille africaine de racines et tubercules.L'objectif global de l'étude est d'identifier parmi les 40 génotypes de manioc du germoplasme, ceux qui sont résistants aux infestations de la CART, en vue de proposer les stratégies de protection intégrée à développer dans la culture de manioc.Les essais ont été conduits en milieu paysan dans le Territoire de Beni, Province du Nord Kivu en RDC sous deux types d'altitudes (800 m à 1200 m) et (1200 m à 1400 m). Les sites d'étude et les coordonnées géographiques sont consignés au tableau 1 et à la la photo 4. Le Territoire de Beni étant situé à cheval sur l'Equateur, une grande partie bénéficie du climat équatorial de type Af, de la classification de Koppen, caractérisée par une pluviométrie mensuelle du mois le plus sec supérieur à 60 mm [16].Les conditions climatiques ayant prévalu pendant la période expérimentale en première année (2009) et en deuxième année (2010) sont résumées sur les Fig. 1, Fig. 2 et Fig. 3. L'approche scientifique utilisée dans cette étude est l'expérimentation au champ dans le milieu paysan. Les variétés de manioc ont été plantées en bloc complètement randomisé. Le bloc avait une dimension de 21 m X 13 m. Dans chaque ligne dans le sens de la largeur six plants d'une variété de manioc étaient plantés aux écartements de 1m x 1m suivis sur la même ligne par six autres plants d'une autre variété.La méthode d'infestation était naturelle et s'opérait par les évaluations. Les observations sur la population des cochenilles africaine des racines et tubercules (CART) de manioc se faisaient à un rythme trimestriel sur le manioc âgé de 3 mois après plantation (3MAP), 6 mois après plantation (6MAP), 9 mois après plantation (9MAP) à la première année et jusqu'à 12 mois après plantation (12MAP) à la deuxième année de criblage pour l'évaluation de la production en racines Pour évaluer la population de CART, nous avons procédé au dénombrement des individus à tous les stades de vie (larves 1, larves 2, adultes, individus morts) sur les boutures mères, les racines tubéreuses et nourricières. La technique de comptage a consisté d'abord à séparer à l'aide des machettes et sécateurs les tiges sur les souches, les racines tubéreuses des autres non tubérisées sur les plants à évaluer et ensuite le comptage de CART. Au total, nous avons dénombré les populations de CART sur 80 plants par bloc aussi bien au premier criblage qu'au deuxième criblage pour tout le matériel végétal à chaque évaluation. Pendant le dénombrement nous avons utilisé les loupes entomologiques à agrandissement type Optivisor (optical Glasbinocular magnifier) pour dénombrer les larves 1 et larves 2 de la CART de petites dimensions. Nous avons recouru aux compteurs manuels rapides pour dénombrer les jeunes larves, les adultes et les individus morts. Pour indiquer la tolérance de certaines variétés à la CART, le nombre des racines commercialisables obtenu par chacune d'elles à chacune des évaluations a été pesé et extrapolé en Tonnes par hectare. Une racine est dite commercialisable lorsqu'elle est grosse avec un bon calibre, sans trous laissés par la CART et peut être vendue pour la consommation.Les analyses statistiques ont été faites à l'aide des logiciels JMP, SAS Institute pour l'analyse de la variance et XLSTAT 2015 pour l'analyse en composantes principales (ACP) et pour la classification ascendante hiérarchisée (CAH). Les données de comptage de la CART ont été transformées par la fonction logarithmique.Les résultats de comptage CART ont été exprimés en nombre d'individus par plant. Les populations de CART sur chacune des variétés sous moyenne et haute altitude pendant les échéances d'évaluation en première et deuxième année de criblage sont consignées aux tableaux 3 et 4. Les rendements en racines tubéreuses et les populations CART à la fin du criblage sont résumé au tableau 5. La figure 5 représente la structuration des quatre variables liées à la population CART et à la production en racines tubéreuses en analyse en composante principale (ACP). La figure 5 représente le dendrogramme de rapprochement entre les génotypes de manioc améliorés et locales sous criblage en moyenne et haute altitude sur base d'infestation de CART et rendement en racines tubéreuses en classification ascendante hiérarchisée.Le tableau 3 donne le nombre moyen de cochenilles radicoles par plant sur chaque variété de manioc à 3MAP, 6MAP et 9MAP dans le Territoire de Beni en moyenne et haute altitude en première année de criblage. Les clones de manioc MM96/0105, MM97/2206 et 20B28 paraissent les plus colonisées à presque toutes les échéances d'évaluation, pourtant leur productivité est bonne au vu des nombres des racines commercialisables observés (Tableau 4). L'exploitation de ces derniers génotypes de manioc dans le Territoire de Beni peut être comme une stratégie de lutte contre la CART. Globalement, les variétés et clones de manioc tels que Liyayi, 20B29, 20B1, Mbayilo, Mukalasa, 20B27, MM96/0105, MM97/2206, 81, Butamu et MM96/5272 ont été identifiés comme tolérants à la CART dans les zones agroécologiques situées en moyennes altitude de Beni à cause de leur productivité en racines tubéreuses (Tableaux 5).Le tableau 3 donne également les résultats de l'évaluation de la population CART à l'altitude de 1200 m à 1400 m en première année. Il se dégage de ces résultats que toutes les variétés ou clones criblés en haute altitude ont été faiblement colonisés par la CART à toutes les périodes d'évaluation (3MAP, 6MAP, 9MAP). Toutefois, à 6MAP nous avons observé une population plus élevée de CART, soit une moyenne équivaut à 38 CART par plant par rapport à 3MAP et 9MAP. La moyenne de la population cochenille a été de 31 CART par plant à l'échéance de 9MAP et de 24 à celle de 3MAP prouvant en suffisance que sous haute altitude l'infestation de clones ou variétés de manioc par la CART a été très faible.Aux regards de ces résultats, certaines variétés de manioc qui ont été sensibles à la CART sur les zones des moyennes altitudes ont présenté une bonne production en racines tubéreuses commercialisables et peuvent faire l'objet d'exploitation sur les zones de hautes altitudes (1200 m à 1400 m) du Territoire de Beni. L'amélioration de la productivité de ces génotypes sous hautes altitudes peut être attribuable au fait qu'il n'y a pas jusqu'ici une grande population de CART qui peut causer des dégâts entraînant la réduction du rendement en racines tubéreuses. La population de CART observée sous ces zones reste encore en équilibre avec les pratiques culturales utilisées. D'une manière générale les clones et variétés de manioc ci-après : 20B1, 20B2, 20B4, 20B16, 20B27, 20B28, 81, MM96/4653, MM96/5475, MM96/2100, MM97/2206 et Mvuazi, Dinsaka, Liyayi, Mukalasa, Mbayilo et Balulu ont été identifiés comme tolérants à la CART sur les zones de hautes altitudes du territoire de Beni. Parmi ces derniers génotypes de manioc cinq ont été précédemment identifiés comme tolérants aux dégâts de CART en moyenne altitude.Il s'agit de 20B1, 20B27, 2028, 81 et la variété locale Mbayilo. Ces cinq variétés peuvent être recommandées aussi bien en moyenne qu'en haute altitude dans le territoire de Beni à cause de leur production en racines tubéreuses.Les résultats sur l'évaluation de la population CART à l'altitude de 1000 m à 1200 m et de 1200 m à 1400 m en deuxième année se trouvent au tableau 4.Il ressort de ces résultats qu'à 6MAP, 12 variétés sur 40 de manioc en moyenne altitude ont hébergé une population CART élevée par rapport à 3MAP et 9MAP. La population moyenne de CART par plant à 6MAP a été de 38,9 individus. Egalement à 9MAP il apparaît huit sur 40 variétés de manioc avec une population élevée de CART par rapport à 3MAP. La moyenne de cochenille par plant à 9MAP a été de 36 individus. La population moyenne générale a été de 28,3 CART par plant pour toutes les échéances d'évaluation.La variété locale Kinyoka et la variété améliorée Nsansi ainsi que le clone 20B17 ont été faiblement colonisés par les cochenilles radicoles à toutes les échéances sur les toutes les zones agroécologiques du Territoire de Beni en premier et en deuxième criblage. Malheureusement ces variétés ont accusé de faibles productions par rapport à celles fortement infestées. Les moyennes des populations des individus hébergés par ces trois génotypes de manioc variaient entre 5 à 28 CART. Les variétés améliorées et locales telles que Liyayi, Butamu, 20B27, 81, MM96/0105, MM96/5272, MM97/2206 et Mbayilo ainsi que Mukalasa ont été plus colonisées par la CART. Par contre elles ont présenté une résistance dite « tolérance » à la CART au vu de leur productivité en racines tubéreuses (tableau 5) et pourront valablement servir dans les éventuels programme de création ou d'amélioration variétale. Les résultats sur l'évaluation de la population CART à l'altitude de 1200 m à 1400 m en deuxième année se trouvent également au Tableau 4. Il ressort de ces résultats que la population CART en haute altitude en deuxième année a été très faible pour toutes les échéances d'évaluation (3MAP, 6MAP et 9MAP) par rapport à la première année de criblage. Toutefois, à 9MAP il apparaît un grand nombre de variétés avec une population un peu élevé de cochenilles radicoles par rapport à 3MAP et 6MAP. Il y a eu moins d'individus à 3MAP et à 6MAP avec les moyennes d'infestation respectives de 7,6 et 9,5 CART par plant. Globalement 20 sur 40 des génotypes de manioc criblés sous les zones agroécologiques de hautes altitudes du Territoire de Beni ont présenté une tolérance à la CART, car leur production en racines tubéreuses a été acceptable et comprise dans l'intervalle des rendements des variétés en diffusion en RDC (Tableaux 1 et 5). Ainsi, quatre sur cinq variétés locales ont donné une bonne production en racines tubéreuses à la fin du criblage (12MAP) ; il s'agit des variétés Mukalasa, Mbayilo, Balulu et Kimbambu. Egalement, trois sur sept variétés améliorées en diffusion ont produit une grande quantité des racines tubéreuses de manioc à 12MAP, en l'occurrence des variétés Mvuazi, Dinsaka et Liyayi. Les rendements extrapolés à l'hectare des ces dernières variétés ont été respectivement de 63,8T/ha, 36,4T/ha et de 24,4T/ha. Nous avons aussi observé que 13 sur 28 variétés améliorées en développement ont produit plus des racines commercialisables dans l'intervalle des rendements des variétés en large diffusion en RDC. Ces variétés en développement étaient entre autre 20B1, 20B2, 20B4, 20B16, 20B27, 20B28, 20B29, MM96/4553, MM96/0105, MM96/2100 et MM97/2206. Les rendements extrapolés de ces clones ont varié de 24T/ha (pour MM96/2100) à 75T/ha (pour 20B1).Globalement, pour toutes les zones agroécologiques du Territoire de Beni, les variétés locales de manioc Mbayilo, Mukalasa, Balulu et Kimbambu ainsi que les variétés améliorées en diffusion et en développement telles que Liyayi, Mvuazi, Butamu et 20B1, 20B2, 20B4, 20B16, 20B27, 20B28, 20B29, MM96/4653, MM96/0105, MM96/4653, MM96/2100, MM96/5475 et MM97/2206 ont été à toutes les périodes d'évaluation moins ou fortement infestés par rapport aux autres variétés. Ces derniers génotypes de manioc ont présenté une tolérance à la CART à cause de leur production en racines tubéreuses à l'unité expérimentale. Ils peuvent être considérées comme une stratégie de lutte contre les cochenilles radicoles et révèlent une importance majeure en amont de la filière de mise au point des variétés résistantes à S. vayssierei par les généticiens.Le tableau 5 donne les rendements moyens des différentes variétés de manioc à la deuxième année de criblage (12MAP) sous différentes altitudes du territoire de BENI. Il ressort de ce tableau que les rendements en racines tubéreuses commercialisables les plus élevés a été observés sous hautes altitudes sur les variétés améliorées 20B1, Mvuazi et 20B16, soit respectivement 74,96 T/ha, 63,78 T/ha et 62,94 T/ha. Sous les moyennes altitudes les variétés améliorées Liyayi et 20B29 ont produit 53, 78 T/ha et 30,4 T/ha. Il se dégage de ces résultats que les variétés de manioc criblées dans le territoire de Beni ont été plus productives sous haute altitude que sous moyenne altitude. Cela se justifie par la présence de la cochenille Africaine de racines et tubercule du manioc et ses dégâts qui ont été élevés sous moyenne altitude. Toutefois, certains génotypes améliorés et locales de manioc ont toléré les infestations de la CART en moyenne altitude en produisant des rendements en racines tubéreuses variant entre 20 T/ha et 22,5 T/ha. Il s'agit des clones 20B27, MM96/5272, MM96/2206, MM96/0105 et les variétés locales Mbayilo et Mukalasa.L'ACP construite sur les quatre variables relative à la population de CART et au rendement en racines tubéreuses (3MAP, 6MAP, 9MAP et 12MAP) indique une bonne représentation des variables à travers le cercle de corrélation (figure 5) ; un bon taux de restitution de l'information sur la variabilité totale sur le plan F1, F2 (62,13%) et un étalement presque égale des individus le long des axes F1 qui contient 31,92 % d'information relative à la productivité des 40 variétés de manioc et F2 30,21% d'information sur la population des cochenilles. Variété La cochenille africaine des racines et tubercules (CART) cause des dégâts aussi bien dans les zones de savanes de haute altitude que dans celles de moyennes et de basses altitudes forestières contrairement à ce que rapportait [3] qu'elle était endémique dans les zones forestières humides de basses altitudes. Peut être que chez [3] les études ont été menées uniquement sur les zones forestières. Les rendements en racines tubéreuses des certaines variétés améliorées en diffusion de manioc sous criblage dans les moyennes et hautes altitudes ont été faibles par rapport leur productivité qui varie de 20 T/ha à 40 T/ha dans les zones qui ne connaissent pas les dégâts de cochenilles. Par exemple les variétés en diffusion de manioc telles que Obama, Nsansi, et Sawasawa ont produit moins de 20 T/ha par rapport à leur potentialité productive dans les moyennes et hautes altitudes du Territoire de Beni à cause des dégâts causés par les cochenilles radicoles. Certaines variétés locales et améliorées de manioc ont toléré les dégâts de la CART sous moyenne altitude malgré les fortes infestations de CART. Les variétés locales telles que Mukalasa et Mbayilo ont produit respectivement 22,43 T/ha et 22,53 T/ha sous moyenne altitude. La variété améliorée Liyayi a donné aussi 53,78 T/ha sous moyenne altitude. Ces résultats corroborent à ceux obtenus par [17] sur les effets des infestations de CART sur l'âge des variétés améliorées et locales du manioc en basse altitude à Tshela dans la province du Bas Congo.La classification ascendante hiérarchique des génotypes de manioc criblés en moyenne altitude montre qu'il y a eu des fortes infestations, par voie de conséquence réduction des rendements en racines tubéreuses. Par contre les faibles infestations ont été observées en haute altitude, partant augmentation des rendements de génotypes de manioc. Ceci semble concorder aux travaux antérieurs de [3] où il décrit les basses altitudes comme habitats de ces homoptères.Par ailleurs, il est bien établi que les interactions plantes-insectes depuis plusieurs centaines de million d'années, ont conduit à un processus de coévolution. Ce processus évolutif a permis aux plantes de synthétiser des métabolites secondaires bioactifs vis-à-vis des insectes. Plusieurs voies métaboliques telles que les cascades de réactions de phosphorylation et la voie de jasmonates parmi tant d'autres, ont à cet effet été rapporté dans la littérature comme responsables de la production des composés biocides par les plantes infectées [18]. Il faut en outre noter que l'expression des gènes qui contrôlent la biosynthèse de ces métabolites secondaires serait modulée par des facteurs environnementaux tels que le climat, la nature géologique du site, l'altitude, etc. [19,20]. Si cette affirmation n'est pas correcte, alors, on devrait s'attendre à ce qu'une variété améliorée de manioc testée résiste de la même façon à la cochenille Africaine de racines et tubercules (Stictococcus vayssierei Richard) sur tous les sites testés. Hors, nous avons observé dans la présente étude que le manioc résisterait mieux en altitude. Il pourrait s'agir dans ce cas d'une modulation épi-génétique et donc le(s) gène(s) de résistance à la cochenille seraient inductibles.Eu égards à ce qui précède le changement de certains facteurs climatiques entre autres la pluviosité serait la cause de cette différence de résistance. Toutefois, les études sur l'indicibilité des gènes de résistance de manioc combinées aux facteurs climatiques et écologiques de la CART s'avéreraient indispensable afin d'identifier les facteurs qui seraient à la base de différence de résistance dans différents sites du territoire de Beni.","tokenCount":"3638"} \ No newline at end of file diff --git a/data/part_6/0200266089.json b/data/part_6/0200266089.json new file mode 100644 index 0000000000000000000000000000000000000000..0f2a758f23439e968f94797d5895495b92cd586f --- /dev/null +++ b/data/part_6/0200266089.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"3f48eebcc3fe75e1848f1c7750bbf9b3","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/376d31a3-01b1-44b4-bc0f-8ce953f7e50e/retrieve","id":"916691679"},"keywords":["Distance-based learning","radio","climate change","Philippines","adaptation DWDA Station Manager","and Mrs. Catherine Jimenez, Communication Specialist, for their coordination, facilitation and secretarial support"," The Station Managers of the DA-RFO2: Engr. Fidelino R. Cabantac (Quirino"],"sieverID":"b7ae31fa-cf1b-4d2c-a6e9-717d2d5317ee","pagecount":"66","content":"This work was implemented as part of the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), which is carried out with support from CGIAR Fund Donors and through bilateral funding agreements. For details please visit https://ccafs.cgiar.org/donors. The views expressed in this document cannot be taken to reflect the official opinions of these organisations.. Farmer listeners' intent to participate in other school-on-the-air program .. Table 15. Farmer-listener graduates' suggestions on future SOA program features ...One global issue threatening many country's efforts towards sustainable development is climate change. An unprecedented increase in greenhouse emissions has led to increased climate change impacts. It poses great challenges for the rural poor in developing countries that tend to rely on natural resources for their livelihoods and have limited capacity to adapt to climate change (Smit and Piliphosova 2001;UNFCCC 2007). Globally, climate change is attributed mostly to the changes in temperature, changes in precipitation, sea level rise and the melting of ice and snow in the Northern hemisphere (Intergovernmental Panel on Climate Change, 2009).Long-term changes in climate variability and extreme weather-related events are already evident in many parts of the world. It has become increasingly clear that even serious efforts to mitigate climate change will be inadequate to prevent its devastating impacts that can reverse many of the economic gains made in the developing world in recent decades.In the Philippines, the climate change phenomenon is often associated with extreme weather disturbances such as typhoons and floods, which, in turn, affect many other sectors of the economy. With 50.3% of its total area and 81.3% of the population vulnerable to natural disasters, the Philippines is considered a natural disaster hotspot. About 85.2% of its USD 86 billion annual gross domestic product is endangered, as it is located in areas of risk (World Bank 2008). Since 2000, about three million people have been affected by various disasters annually. The vulnerability of the Philippine agriculture sector to climate change has been acknowledged to be substantial as it is the most vulnerable among Asian countries like Thailand, Indonesia and Vietnam when it comes to floods and storms (ADB, 2012).In a study, Vista (2014) reported that climate-induced impacts will result in a net loss to the Philippine economy and its key agricultural sectors in the short run. Since production would be greatly affected and would have ripple and multiplier effects in the economy, it is imperative for Filipino farmers to employ adaptation measures to lessen the impacts of climate change.There is increasing urgency for a stronger focus on adapting agriculture to future changes in the climate. There are many potential adaptation options available at the management level, often variations of existing climate risk management. The management-level adaptation options are largely extensions or intensifications of existing climate risk management or production enhancement activities in response to a potential change in the climate risk profile.One of these include the cropping systems. There are many potential ways to alter management to deal with projected climatic and atmospheric changes. Citing several experts, Howden et al. (2007) enumerated varied adaptations to include: Altering inputs such as varieties/species to those with more appropriate thermal time and requirements and/or with increased resistance to heat shock and drought, altering fertilizer rates to maintain grain or fruit quality consistent with the prevailing climate, altering amounts and timing of irrigation and other water management. Wider use of technologies to \"harvest\" water, conserve soil moisture (e.g., crop residue retention), and use and transport water more effectively where rainfall decreases. Managing water to prevent water logging, erosion, and nutrient leaching where rainfall increases. Altering the timing or location of cropping activities. Diversifying income through integration with other farming activities such as livestock raising. Improving the effectiveness of pest, disease, and weed management practices through wider use of integrated pest and pathogen management, development, and use of varieties and species resistant to pests and diseases and maintaining or improving quarantine capabilities and monitoring programs.  Using climate forecasting to reduce production risk.However, there are yet relatively few studies that assess both the likely effectiveness and adoption rates of possible response strategies. Against the backdrop of rapidly changing weather conditions and the severity of the impact on poor subsistence farmers, it is urgent that the different options available to farmers to cope are documented. A good understanding of how they can be widely adopted is critical. This understanding includes the adaptation options that farmers may have access to, their perception towards them, and the determinants to adopting them.One development strategy thought of by the Philippine Department of Agriculture (DA) to inform the rice farmers about the effects of climate change and how to control its impact to rice farming is the use of the radio. As argued in a manual co-developed by DA Regional Field Office 02 (DA RFO2), despite the prevalence of high-speed Internet and television in today's world, radio is still a critical and relevant medium especially in developing nations (DA RFO2, 2018). Citing the data from the United Nations Educational, Scientific and Cultural Organization, more than 95% of the world's population uses radio, as compared to roughly one-third of the global population with Internet access.Philippine data shows that radio reaches 85% to 90 % of the population, while TV reaches less than 60%. For this reason, radio is considered the most reliable medium for sharing news in the countryside. The document further argues that radio transcends literacy and geographical barriers and being in audio mode, elicits strong emotional impact among listeners. Along with this, the \"Kaalamang Pagsasaka sa Himpapawid,\" a school-on-the-air Consortium, and other national and regional agencies in the region.The SOA-CSA program contained 68 modules broadcasted in 14 radio stations within the Cagayan Valley Region. About 10,000 rice farmers enrolled in the program, but only around 5,000 graduated. How the graduates used their knowledge on CSA, which they acquired from the radio program is the intent of this study.The study assessed the intermediate outcomes of the SOA-CSA program on farmer-learners in Cagayan Valley in the adoption of climate-smart rice technologies. Specifically, it was intended to accomplish the following objectives: The study is anchored on the theory that radio influences the rice farmers' knowledge and adoption of CSA technologies (Fig. 1). It is viewed that if the delivery of the program was effective, it could be evident in the awareness and knowledge of the causes and effects of climate change and the level of knowledge and adoption practices of the rice farmers taught in the radio program. It is assumed that the rice farmers who intently listened to the program and completed all episodes have higher level of awareness and knowledge of the climate-smart technologies.Consequently, these rice farmers are likely to adopt these technologies in the rice production practices that address climate change concerns. Adoption of climate-smart technologies is considered as a factor to the yield and income differences among farmers. The study used mixed research methods, i.e., the use of both quantitative and qualitative research strategies to gather the needed data to achieve the research objectives. The quantitative component of the study involved the collection of data through a structured questionnaire adopted from different sources. The qualitative aspect of the study involved the conduct of focus group discussions (FGDs) and key informant interviewing of selected groups and partner agencies.The study was conducted in four provinces (Cagayan, Isabela, Nueva Vizcaya and Quirino) in the Cagayan Valley Region, particularly in municipalities within the broadcast reach of the 14 radio stations that aired the SOA-CSA materials. The population of the study was composed of all the graduates of the SOA -CSA program.Out of this population, an initial sample of 376 graduates was stratified across the provinces, with the most number coming from provinces that had the biggest number of graduates. The criteria for being selected in the study include: regular listeners; model farm participant; and resident of the area with good reception of the radio station broadcasting the SOA-CSA.Using the Slovin's formula to determine sample size and the proportional allocation technique, the samples of the study were distributed in the provinces shown in the table below. In the actual data gathering in the sampled towns, the list of graduates was inadvertently misplaced at the Municipal Agriculture Office (MAO). Thus, the sampling technique was altered. The research team used snowball sampling method to reach the other graduates in the sampled barangay. After locating and interviewing the first sample with a graduation certificate, names of other graduates from the barangay were identified. In several sampled barangays, the number of samples was not met owing to their ineligibility based on the inclusion criteria, or eligible respondents were out of town during the visit; no call back was made due to time and financial constraints. For this reason, the actual samples were 352.On the other hand, a total of 24 farmer leaders participated in the FGDs held separately in the four provinces. Some of the invited farmer leaders, who also hold positions in their communities, failed to come because of varied reasons. Nevertheless, the participants actively joined the discussion on the issues and concerns raised during the FGDs, which lasted for about one hour and thirty minutes.Two research instruments were used to gather the needed data. Afterwards, for the level of awareness, farmers were asked if they were aware or not of the causes and effects of climate change, and then were asked on the level of knowledge they have about each of the causes and effects, using a three-point Likert scale: Little, Much, Very The second instrument is the Focus Group Session Guide, the tool to guide in the discussion with farmer leaders (Annex B). The questions elicited from the FGD members their attitude, opinions, and ideas on the circumstances of their involvement in the SOA and future SOA programs, their knowledge and utilization (including sharing) of the climate-smart technologies, the problems related to their adoption, and the SOA program content and features they preferred.The October 2019. The composition of the FGD was from four to six. Using the FGD SessionGuide, the questions elicited responses on the members' attitude, opinions, and suggestions on the SOA learnings and utilization, as well as their preferences on future SOA programs and their features. The lead researcher facilitated all FGD sessions in the provinces.Data were encoded in EXCEL file to have ease in data encoding, cleaning, and manipulation.After data cleaning, the file was imported into the Statistical Package for Social Sciences software.To categorize and describe the group, frequency count, mean, and standard deviation were computed. General responses in variables measured by Likert scales was interpreted by computing the weighted mean per statement. The arbitrary scale points to interpret the weighted means are the following:For Level of Knowledge:Level of Knowledge Farmer-listener graduates (FLGs) are predominantly males (69.9%); the rest (39.1%) are females. This trend characteristically describes the Filipino farmer in terms of gender, where there are more males (89%) than females (Census on Agriculture and Fisheries, 2002). In 2015, the proportion of females in the agricultural work force was 25.7%. It indicates that in the region, more females are joining the agriculture workforce.In terms of age, FLGs are in their adult stage, as shown in the mean age of 52.4 with a standard deviation of 10.4. The youngest is 24 while the oldest is 80. Moreover, the fact that there are farmers who listened to the radio affirms the value of lifelong learning to a farmer interested to improve his or her farming practices.This group of FLGs is younger than the national average age of Filipino farmers at 57, according to Director Asterio Saliot of the DA-Agricultural Training Institute (ATI) (The New Humanitarian, 2013). However, the 2017 survey of DA revealed that the farmers' average age is 60. Such trend is worrisome to the Department as this implies that young Filipinos are no longer interested to take on farming as an industry (Inso, 2018). More than three-fourths (86.1%) of the FLGs are married. This fact gives them more motivation to improve their rice production to meet the needs of the family members. In the FGD with farmer leaders, they claimed that this expectation was anticipated in the learning acquired from listening to the SOA-CSA program. They want new technologies that can increase their rice yield.In terms of educational background, the majority (54.6%) of the FLGs has reached elementary and high school levels of education. It means that they are capable of processing information heard over the radio. This educational trend among the famer-listener graduates in the region is far better than the reported national figure of one-third of agriculture workers who did not finish primary school (Briones, 2017).The reported income of the farmer-listener graduates ranges from a minimum of PHP 50,000(among the small landed farmers) to the maximum of PHP 1,500,000 (among owners of big farms). The median income is PHP 80,000, which is way below the average income of farmers (farm, off-farm, and non-farm) that is around PHP 100,000. Comparing it to the 2015 poverty line of PHP 108,800 set by the National Economic and Development Authority, most of the farmers could hardly make both ends meet. Nevertheless, the farmer leaders explained during the FGD session that they can cope with their financial needs by engaging in subsidiary livelihood activities. As they are dependent on farming, whatever problems encountered in their production activities impact on their financial capability to survive.Table 2 shows the biophysical characteristics of FLGs. Farm ownership for rice ranges from one-fourth of a hectare to 13 hectares. On the average, they till about 1.97 hectares (SD = 1.66). On the other hand, the area farmed for other crops ranges from one-fourth to five hectares; the mean is recorded at 1.57 (SD = 0.13).As indicated, some farmers own a small farm while others own large farm areas. In a report, Koirala, et al. (2014) However, the figures are below other farmers' use of more advanced hybrid varieties (e.g., SL-H8) that yield from 160 to 245 cavans per hectare of irrigated land (Gomez, 2019).In the FGD sessions, farmers attributed their rice yield to the practice of recommended technologies, especially seed selection. However, the variations in the farmers' reported rice yield is attributed by experts to different factors such as genetics, agronomy, and climate variability brought by climate change (Lesk et al., 2016;Battiste & Naylor, 2009;Urban et al. 2015). Farmers also cited that even if they use high-yielding varieties, the expected economic benefit is negated by natural calamities such as typhoons, flooding, and market circumstances. The topics most FLGs listened to are along cultural management practices of rice, namely: variety and seed selection (96%); land preparation and water management (94.6%); nutrient practices and pests and diseases management (93.2%); postharvest operations (92.9%); harvest management (92.3%; crop establishment (90.9%); and impact of climate change on agriculture and food security. Most topics listened to by the FLGs were on the cultural management aspects. Evidently, they are interested to learn about the rice technologies that are critical in the growth of the plant to ensure better yield.In the FGD sessions, farmer leaders considered the information they acquired from the SOA-CSA program important to them as these influence the yield of their rice crop. Their realization of the impacts of climate change on their farming and the availability of food for them and the community added more reason for them to learn climate-smart technologies. In addition, they conveyed that their motivation to listen was for them to upgrade their adoption of technology that could give them more economic benefits. They answered affirmatively when asked if their expectations in listening were met.As evidenced by the data, not all FLGs were able to listen to all the topics. Only 169 or 48% of them listened to all the 24 topics; others missed a few of the topics owing to various reasons. Farmer leaders pointed out several factors for their inability to miss the broadcast: they were out of town;  the radio signal was poor, sometimes interrupted by intrusion of foreign language radio programs; and  they moved to the farm before the program was aired.However, those missing the broadcast inquired from their fellow farmer listeners what the topic was and what significant information was discussed. Others with cellphones asked a member of the family to record the program for them to listen to it later. Other listeners volunteered to share the information to those who missed the program, as well as on other farmers of the community who were not regular listeners. To some with cell phones, they visited the DWDA Facebook page to listen to the uploaded episodes they missed. Nearly all (92%) of the FLGs listened to the SOA-CSA program through their own radio sets.Those without radio sets (4.5%) listened to the program through the neighbor's transistor radio set, while an identical 1.7% of them with cellular phones either listened through the radio applications of their unit or listened through live streaming of the program on Facebook.They listened mostly at home (93.8%), while 3.4% brought to the farm their radio set and listened to the program as they undertook their farm chores. Others (2.9%) listened to the program with their neighbor's and through their cellphone unit where they were at the time of the broadcast. The use of these strategies attests to the FLGs' desire to learn CSA for rice. In some instances, listening to the radio program was a family affair. If the farmer is out, the wife or a child was requested to listen and share the information to the farmer later. This On the level of knowledge on the effects of climate change, the trend shows much knowledge, with an overall weighted mean of 2.02. FLGs benefitted from their listening to the SOA-CSA program. In the FGD sessions with the farmer leaders, whatever little knowledge they have before listening to the program was increased by the information they heard. They have now a better appreciation of stopping their traditional practices (like burning of rice straw in the farm) that they realize contribute to the problem of climate change. If they persisted with these practices, they explained they would be disadvantaged with continued episodes of getting lower farm yield.FLGs were asked about their level of knowledge of the CSA technologies for rice, as well as their level of adoption. Consistently, these same technologies are the ones adopted almost always by them, except for the use of farm machinery that is used always.In the FGD sessions, farmer leaders pointed out that they adopted semi-farm mechanization or partial adoption. Farm machines are used during land preparation and harvesting while manual labor is used in pulling seedlings, transplanting, and harvesting palay that lodged heavily during windy heavy downpour. They explained the non-use of seedling transplanter because of they are not commonly used by them.On applying the 3 Rs of solid waste management, farmer leaders related how the golden snails collected from the rice farm are used in vermicomposting. They used vermicompost as basal fertilizer. They have learned not to burn the rice straw; rather, they spread them on the farm. They explained that if properly decomposed the farm is supplied with 5% of free urea already.They also explained the advantage of synchronous planting in reducing pest occurrence.Moreover, they shared the indigenous technology of laying madre de cacao (Gliricidia sepium) leaves on the farm for a while then draining the farm to drive away insects and corn plant hopper. Cutting branches of the tree and setting them in strategic areas of the farm also attains the same purpose. This practice manifests farmers' ability to blend tradition and science. On integrated pest management, farmer leaders shared their practice of observing the presence of insects in the farm. Farmer leaders attested that maintaining the paddies clean is effective in keeping insects and rodents away. If there are only two or three insects in a one square meter area, they believe that there is no need to spray pesticides. Instead, they prepare a concoction of pepper-Antibac fabric softening liquid-kerosene gas for the spot spraying to get rid of them.Encouraging information that arose from the discussions is the radiation or diffusion effect of farmers' adoption of recommended technologies. Farmer leaders confirmed the \"wait and see\" approach of some farmers; if they do not see the benefits of a technology, they are hard to convince to adopt it. Cited as example is the non-burning of the rice straw after the harvest.Setting them on the rice farm and plowing them over during land preparation makes the soil more fertile.As the non-adopting farmers realize its benefits, they begin inquiring from the farm leaders who are willing to share their learning. Likewise, farmer leaders narrated how they convinced other farmers of the advantage of transplanting one seedling per hill against the traditional practice. They claimed that they were able to convince non-listener farmers who found that there is less need of seeds when using hybrid seeds (15kg/ha) versus the inbred seeds (40 kg/ha.). In a research by Wanda ( 2016), agricultural radio programs in Africa have influenced farming activities and through the adoption of new ideas, farmers become economically empowered.The technologies sometimes adopted by the FLGs are along income diversification (2.46), crop diversification (2.42), using different cropping systems (2.28), and mixed cropping. The finding reflects the intent of the FLGs to have multiple sources of income that are not totally dependent on rice production. In the FGD sessions, they expressed the desire to learn farm diversification, including the acquisition of value-adding skills for their main product.The primary problems related to the adoption of climate-smart rice technologies are natural calamities (68.8%), lack of financial resources (67.3%), and lack of support from agencies (51.4%). Whatever inputs they invest to get more yields from the farm is dependent on the weather conditions. Farmer leaders, in the FGD sessions, revealed that at times their yield is reduced when drought and typhoons hit their area. At the flowering stage of the rice plants, rain showers between 9AM and 1PM are mentioned by the farmers to be detrimental to their rice plants. Strong winds at this stage, they claimed, are also unfavorable because pollens in the spikelets are blown away. On the other hand, typhoons during the maturity stage of the rice plants are disadvantageous for farmers to gain more profits. They alleged that when rice plants lodged due to flooding, the combined harvester could not be used. Under such situation, grains are submerged under water, resulting to the blackening of the grains and at worse, their germination. Consequently, when it is sold, it is cheaply priced.In terms of financial resources, farmer leaders narrated the red tape involved in securing loans, like the many documents to prepare and the certifications to secure. For instance, in the Agricultural Competitiveness Enhancement Fund loan offered by Landbank, loan applicants are required to submit documents, namely: MAO certification, Farm Plan, Notarized Statement that they do not have current loans, and Tax Information Number from the Bureau of Internal Revenue. Seemingly, the farmers do not appreciate the concern of the lending institutions to assure them of their ability to repay loans. On the farm mechanization loaning program, on the other hand, they claimed that it is only through a cooperative that it could be availed. The documentary requirements to be submitted are discouraging the farmer groups to secure this type of loan program.Clarifying their problem on lack of support from government agencies, some farmer leaders related that at times, the distribution of certified seeds and fertilizer is delayed. Related to this problem, farmers commented on the practice of government technicians in asking what rice variety they want, not based on the characteristics of the rice farm.Likewise, they are allegedly asked to subject the farm for soil analysis, but results are not delivered on time, keeping the farmers from following the recommended fertilizer needs. At times, though, the reason for the delay is their last-minute submission of the soil prior to land preparation. In addition, some farmer leaders explained their difficulty of managing farm weeds because irrigation water is not available at times. Those situations force them to apply weedicides.In the FGD sessions, some farmers suggested that the provision of support to the farmer listeners should not end with the graduation; there must be a follow-up to determine the technology application of the graduates. They should not be left to fend for themselves.Another suggestion is the need to time the airing of the SOA program before the onset of their Two government agencies are identified by the FLGs to be providers of support in their adoption of recommended rice technologies; these are the MLGU through the MAO (82.7%) and DA RFO 2 (76.7%). Nearly half of the farmer-listener graduates also mentioned the National Irrigation Administration (NIA), (49.4%), ATI (46.6%), and Philippine Crop Insurance Corporation (PCIC) (44.9%) that are supportive of them. The continuing program of the municipalities to provide seed and fertilizer subsidies has made the FLGs aware of the LGU support to them. Moreover, during the farm visits, the municipal agriculture extensionists provide farmers with technical assistance in their farming activities. Although the MLGUs are just conduits of the DARFO 2 in implementing agricultural productivity programs, farmers are unaware of the fact that it is the regional office as the main provider of the free inputs and technology disseminated to them by the MLGU.FLGs also recognize the free irrigation water provided by NIA in their respective areas.Similarly, they are aware of the crop insurance they benefit from through the PCIC.On the other hand, more than one-fourth of the farmer-listener graduates took cognizance of the support given by the PLGU (39.8%), Philippine Rice Research Institute (28.7), and Radyo ng Bayan -DWPE (28.4%). Because of their non-proximity to the farmers, the other agencies have no opportunity to be accessed by the farmer-listener graduates. State universities in the area, through their extension program, also extend support to the farmers in various ways.As for the other agencies cited by farmers, they could be referring to their involvement in the SOA-CSA. During the orientation, farmers were informed of the many agencies collaborating in the program.The study wanted to identify the various sources of information from which the FLGs derive their knowledge. Table 10 shows that DA RFO2 remains to be the main source of knowledge of the farmers through various modes. The SOA-CSA program (70.7%) was identified because of its recency in implementation. In the FGD sessions, farmer leaders estimated that their knowledge on CSA for rice technologies due to the SOA would border from 70 to 80%.This finding proves the effectiveness of the SOA as a mode of informing farmers of any agricultural development beneficial to them, especially on CSA, as the topics were devoted on it. The power of the radio as vehicle for agricultural development is asserted by Myers (2008) in his research that pointed out that unlike television and newspapers, radio is still the most popular and widely used medium of communication. The radio has also helped bridge the digital divide by providing an opportunity for sharing information limitlessly. The regular modes of information dissemination of DA RFO2 are also identified. These include flyers and pamphlets from DA and other agencies (58.8%); seminar-workshops (72.4%); meetings/fora (54.4%); and field days (54%). As part of the network of service providers, the involvement of the ATI with its season-long farm school was acknowledged by the FGD participants. This means that DA remains as the primary source of information of the farmers.As a partner agency in agricultural development, the extension workers from MAOs (71%) are cited as the major sources of what they know about climate-smart rice technologies.Considering that most agricultural development programs implemented by DA RFO2 are downloaded to the local government units, the farmers took cognizance of the important role of the MAO as an information provider.Majority (58.8%) of the farmer-listener graduates pointed out the assistance also provided by the Local Farm Technicians (LFTs). In the FGD sessions, nearly all the LFTs in their area share the information they learned to their fellow farmers. This finding attests to the advantage of deputizing and empowering LFTs because they are the most accessible technicians in their own communities. The monetary monthly incentive of PHP 3,000 for the eligible LFTs who complete requirements is mentioned as additional motivation for them to sustain their efforts as farm technicians.Inquired about the usefulness of the information learned from the SOA-CSA, the trend shows that they were useful to the farmer-listeners. Majority (55.7%) considered the learning useful, while 36.9% believed it was very useful. About 7% thought it was somewhat useful.Participants to the FGD sessions related the importance of knowing the causes and effects of climate change, especially the ways to reduce the risks in their rice production activities. They mentioned that their learning has made them cope with the uncertainties of farm production owing to unpredictable climatic conditions in their area. They explained that the usual cropping pattern has changed, pushing them to learn alternative options.Among those who found their learning useful, 53.4% were moderately influenced while 28.9% were fully influenced. Only 16.8% were slightly influenced. FGD participants mentioned that they have been convinced that adopting climate-smart rice technologies is indeed beneficial to them. From their initial use of the technologies they heard in the SOA-CSA program, they had observed their effects to their crops and consequently their yield.With the initial observations, they claimed that such technologies are relevant to them. Economic Benefits of Farmers' Listening to the SOA-CSAIn terms of economic benefits, about 96% declared that there was an observed increase in farm yield (Table 12). About 43% indicated they had much increase in their yield while 17% considered the increase just enough. The rest noted a little increase. Indeed, the SOA-CSA initially contributed to approximately 19-cavani increases per hectare in rice production by farmers who adopted the recommended technology.Moreover, the yield is higher among the FLGs who adopt more technologies, particularly the use of high-yielding hybrid varieties (e.g., SL8, Bigante, Pioneer) resistant to drought and flood, integrated pest management, organic farming, and efficient use of irrigation water. It means that adopting more recommended rice technologies would result to higher yield.Among those who reported lower yield were those using inbred varieties, like RC222, PSB, and RC82. To this variation, it was reported that adopting technologies for sustainable farming systems involves uncertainty and trade-offs. Technologies that can contribute to an economically efficient farm sector and the financial viability for farmers can motivate farmers to adopt them in their farms. Farmers will invest in and implement sustainable technologies and farm practices if they expect the investment will be profitable (Organization for Economic Cooperation and Development, 2001) In the FGD sessions, farmers indicated that the use of hybrid rice requires 15 kg of seeds only, as compared to the 40 kg required in using inbred varieties. The big reduction of input cost is the new practice of setting only one seedling per hill. Furthermore, nutrient competition is minimized, resulting to more grains and consequently higher yield.Because of the preceding circumstances, 96% of the farmer-listener graduates observed an increase in their farm income after the initial use of the recommended technology heard from the SOA-CSA program. The increase was generally much as about 58% of them clustered in the \"much\" and \"very much\" response categories. The mean income increase is PHP 18,345 (SD = P14,319). The finding reveals that the farmers' initial adoption of the recommended CSA technology has benefitted them financially. Other preferred commodities are livestock (54.5%), poultry (46%), and corn (41.8%). In the FGD sessions, the majority of the farmer leaders identified corn as their preferred commodity.In the discussion, however, they conveyed another threat on the profitability of corn production: the market price could be low because high-yielding corn varieties is inputintensive. To have more enrollees in future SOA programs, several considerations should be considered as suggested from the FLGs' experiences. Table 14 indicates that the most preferred time slot by 67.9% of farmers is 5 AM to 6 AM. In the FGD sessions, farmer leaders pointed out that at 5 AM, they are still at home and have time to listen. The Nueva Vizcaya group, however, mentioned that the feasible time for them is 4:00AM as they could listen to the program while they prepare their morning meal. As the sign-in time of most radio stations is 5 AM, they mentioned that 12 noon to 1 PM could be a good alternative. Airing the SOA outside those time slots would reduce listenership as most of them are already in the farm, except those who bring with them their transistor radio. The radio stations with the best reception within their area were suggested by the farmerlisteners. In the FGD sessions, the radio stations with good reception in the Isabela and Quirino provinces, and partly in Nueva Vizcaya, are DWSI (24.6%) and DZNC (2.9%). In addition to DWSI, DZRV (18.6%) in Nueva Vizcaya was suggested by the farmers. In Cagayan, DWPE was suggested by 27.2% of the farmers, while 8.6% identified DWDA. All community radio stations near their areas, although limited in signal coverage, were identified by other farmers.For the farmer leaders, choosing the radio station with the best signal is a major factor for the farmers to listen and to truly benefit from the program. When there are interferences from other radio stations and when the signal is fluctuating, farmers could not pick up the salient information that they need to know. Likewise, they will not be able to retrieve an information easily because a SOA episode cannot be replayed right away. Those with access to social media can review the broadcast they missed.In terms of announcers, the majority (59.6%) suggested a male and female tandem, while approximately 26% favored that both announcers be male. In the FGD sessions, the farmer leaders mentioned that they will listen to any tandem of announcers as long as they can present information clearly. Additionally, they suggested that the program hosts need to have a sense of humor to maintain the listeners' attention. They also desired that they talk slowly and clearly for them to catch up and take down notes.The 30-minute duration of the program is favorable for them. Likewise, they recommended that summary notes of each episode should be distributed to them during the graduation for them to review the lessons and revisit the episodes they missed.As Ilocano is the lingua franca in the Cagayan Valley region, it is identified as the preferred language during the program. Farmer leaders in the FGD sessions mentioned that technical terms in English without Ilocano equivalent was explained by the program hosts during the first SOA-CSA program. This strategy, they said, could still be adopted by the next batch of hosts.Farmer-listener graduates are predominantly married middle-aged men with a high school level of education. They have a median income of PHP 80,000 sourced mainly from farming activities. They till about two hectares of rice land and have more than a hectare for other e. After three years, an impact evaluation of the SOA-CSA should be conducted to assess the long-term impact of the program. In doing so, gender and age segregation of impact could be assessed.f. RAFIS, particularly DWDA and regional office website administrators, to sustain in the archives feature of the regional website or in the Facebook account all episodes of the SOA-CSA program for the FLGs to review and other interested farmers to listen. ","tokenCount":"5887"} \ No newline at end of file diff --git a/data/part_6/0206828659.json b/data/part_6/0206828659.json new file mode 100644 index 0000000000000000000000000000000000000000..e8f66e44e911099e72cf6a86b28baa5fd5d29f0a --- /dev/null +++ b/data/part_6/0206828659.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"c0d930e462163445321ed335ea8c07b4","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/6575414e-25d4-4de8-a7cf-242b462a4c42/retrieve","id":"-887379187"},"keywords":[],"sieverID":"bd18507b-bf26-4c4c-90ca-adb9eda4802f","pagecount":"19","content":"Cattle production in Colombia has an important social and economic role but causes considerable environmental impacts, such as deforestation and greenhouse gas emissions by ruminants, particularly methane. Thus, technological innovations aimed at reducing these impacts must focus on both economic and environmental sustainability. Silvo-pastoral systems (SPS) offer productivity increases while generating environmental benefits and ecosystem services and are therefore at the center of debate around sustainable production alternatives. The objective of this article is to evaluate the economic-environmental performance of two proposed SPS for a cattle fattening system for the Colombian context: (i) Urochloa brizantha cv. Toledo and (ii) Urochloa hybrid cv. Cayman, both in association with Leucaena leucocephala trees for browsing and shade provision. They are compared with the respective base scenarios of only using the grasses in monocultures. The study consists of a financial analysis, which estimates potential profitability increases in beef production in the SPS, and an environmental evaluation, which estimates the monetary values of microclimatic regulation and reduction of methane emissions. The value of methane emission reductions is then integrated into a combined economic-environmental evaluation. Results show that both SPS improve the profitability indicators of the production system and reduce the probability of economic loss. Likewise, the reduction of methane emissions in the SPS is estimated at US$6.12 per cattle, and the economic value of microclimatic regulation at US $2,026 per hectare.Cattle farming is among the most important activities within the agricultural sector in Colombia. According to Fedegan [1], it contributes to about 1.4% of the national, 21.8% of the agricultural, and 48.7% of the livestock gross domestic product of the country. The cattle sector generates employment for more than one million Colombians, mainly in fattening (131,899 jobs), breeding (267, 581), dual-purpose cattle farming (530,739), and dairy farming (138,542). About 23 million hectares of land are used for pastures and forage production and the transformation of native landscapes and forests into pastures generates impacts on the different ecosystems of the country. Over the last decades and in the face of growing international concerns on climate change, environmental degradation, and the role cattle farming holds in that regard [2], the sector has been pushed into the center of debate on environmental impacts in Colombia, particularly on those related to deforestation, greenhouse gas (GHG) emissions, and loss of biodiversity [3].the economic profitability and impacts of cattle production systems has been traditionally focused on the evaluation of implementing improved forages, mostly as monocultures [56,57,68,69,80,90,91], or grass-legume associations [55,92]. Likewise, only few recent studies have focused on providing estimates of the economic-environmental benefits of implementing SPS [93,94].Among the studies that approach SPS from an economic-environmental perspective, Bussoni et al. [93] present a comprehensive analysis that combines the economic benefits of SPS with environmental objectives in Uruguay through three hierarchical models. Model 1 focused on optimizing the combined Net Present Value (NPV) for cattle production (US$302,935) and forestry (eucalyptus timber) (US$556,578). Model 2 focused on prioritizing the cattle NPV (US$317,307) at the expense of a negative balance of carbon dioxide equivalent (CO 2eq. ) emissions (− 20,160 tons). Model 3 focused on minimizing the CO 2eq. emissions (+6,788 tons) in a scenario where environmental aims are pursued at the expense of a difference in the cattle NPV of US$-24,609 compared to Model 2. They conclude that both productive and environmental goals can be integrated, and the environmental goal can be achieved if prioritization happens at a higher hierarchical level. For the State of Sinaloa in Mexico, Cuevas-Reyes et al. [94] found that an intensive SPS with Leucaena leucocephala and Cynodon dactylon is financially viable and that viability significantly increases when the carbon capture potential is considered and monetized (i.e., NPV, IRR, and B/C increase by 15%, 9%, and 2.5%, respectively).Regarding the financial viability of SPS, Carriazo et al. [95], through choice experiments, studied the effect of technical assistance on the adoption of SPS in Colombia and found that ranchers value SPS with technical assistance for cattle production with US$290 per ha/year while the value for improved forages as a monoculture with technical assistance is significantly lower (US$128 per ha/year). Also for Colombia, Enciso et al. [57] estimated the economic benefits of integrating Leucaena diversifolia trees with the grass Urochloa hybrid cv. Cayman in a SPS. Compared to the evaluated monoculture with Urochloa hybrid cv. Cayman, the SPS showed strongly improved profitability indicators, such as for (i) the NPV, which depending on the scenario was up to four times higher in the SPS, (ii) the Internal Rate of Return (IRR), which improved from 11 to 22%, (iii) the Benefit-Cost ratio (B/C), which was positive in all scenarios, (iv) the payback period, which was reduced from six to four years, and (v) the minimum area required for obtaining two Colombian minimum salaries, which was reduced from 6.5 to 3.8 ha. Likewise, the probability of obtaining economic loss was lower in the SPS. Other profitability analyses conducted for SPS with Leucaena leucocephala in Costa Rica [96], the Caribbean region of Colombia [97], and the state of Michoacán in Mexico [98] report significantly higher IRR for the evaluated SPS, oscillating around 33%. In a review of different intensive SPS arrangements in Latin America, mainly with the species Leucaena leucocephala and Tithonia diversifolia, Chará et al. [99] highlight the positive impacts the establishment of these systems have on edible dry matter production, meat and milk production, the reduction of chemical fertilizer needs and feed concentrates, and thus, farm profitability. Braun et al. [64], in a summary on SPS for South America, conclude that SPS are economically attractive alternatives, which allow for deriving different products (e.g., timber, beef, milk, fruits) at different times, and that the inclusion of trees results in a more secure long-term income while the beef component is more oriented towards short-term incomes. Other authors, such as da Silva Santos and Grzebieluckas [100] for Matto Grosso in Brazil, Quaresma Maneschy et al. [101] for Pará in Brazil, Alonzo [102] for Belize, Boscana et al. [103] for Uruguay, Rade et al. [104] for Ecuador, Ramírez-Martínez and Salas-Razo [105] for Michoacan in Mexico, and Bernardy et al. [106] for Brazil, have also provided insights into the financial viability of different SPS setups in Latin America, evidencing positive results in most cases.Moving away from Latin America, other studies can be found for Australia, and particularly Queensland, where Leucaena leucocephala was identified as the most profitable legume for SPS, capable of doubling per hectare gross margins when integrated with perennial grasses [107], and if adopted at the regional level, could lead to economic benefits of US$ 69 million per year [108]. Francis et al. [109], in a study on the financial performance of SPS in Queensland, Australia, also found that the implementation of SPS is financially attractive, i.e., when timber production is incorporated.Although an increasing number of studies has evaluated the financial viability of SPS, only two partially quantify some of the ecosystem services offered by them [93,94], disclosing an important research gap around two topics, namely (i) further evaluating the financial viability of SPS in different contexts and (ii) integrating the monetary values of the offered ecosystem services and environmental benefits in comprehensive economic-environmental evaluations. This, however, raises questions on (i) the type of ecosystem services and environmental benefits SPS can offer, and (ii) the availability of technical data on those ecosystem services that allow for including them into the economic evaluation of SPS.SPS are conceived as multifunctional and dynamic socio-ecological systems, which result from a historical coevolution, i.e., of relationships, feedback, and dependencies, between local communities and their environment. SPS contribute to shaping a diversified landscape structure and configuration that fosters the provision of ecosystem services and environmental benefits to society and the planet [49].In addition to providing environmental benefits, such as the mitigation of GHG emissions [110][111][112][113][114], SPS provide numerous ecosystem services. Several studies [48][49][50][51]115] have documented evidence on the provision of ecosystem services by SPS, such as microclimatic regulation, carbon storage and sequestration, soil conservation, and the creation of habitats for biodiversity. These environmental advantages of SPS have allowed linking incentive programs such as Payments for Ecosystem Services (PES) that seek to promote the conservation of water sources and ecosystems through sustainable production schemes [61,75,[116][117][118][119][120][121] and become a financial option for producers to partially monetize the environmental value of implementing SPS on their farms.In Colombia, Vallejo et al. [122] have studied the benefits SPS have on soil quality and nutrient recovery, and found them to be viable alternatives to improve soil quality and metabolic function, which is reflected in the significant increase in microbial biomass, biomarkers, and enzymes. For their part, Polanía-Hincapié et al. [123] carried out a field study that aimed at evaluating changes in the physical quality of the soil that comes along with the subsequent transition from traditional cattle management to SPS in the Colombian Caquetá department. Their results indicate that the implementation of SPS is an efficient strategy to restore the physical quality of the soil in degraded pastures, and contributes to increasing pasture productivity, while indirectly decreasing the pressure of deforestation in the Amazon basin. Martínez et al. [124] have studied the impacts of SPS on soil quality parameters in degraded soils in the Colombian Sinú river valley, showing that SPS helped in increasing soil pH and nutrient availability. Rivera et al. [51], on the other hand, explored the potential benefits that SPS have on the conservation and biodiversity of ecosystems. They conducted a study on the ant fauna in cattle farms in the La Vieja river basin in Colombia, analyzing the relationships between tree cover and the diversity and composition of ant species in different cattle systems. They found that treeless pastures had less than half the number of ant species than pastures that integrated for example Leucaena leucocephala trees. Mosquera et al. [125] evaluated the effects of land use changes on soil organic carbon, carbon content, and primary forest stocks, by comparing degraded pastures with improved pasture systems and SPS in the Colombian Amazon. Their results indicate that both the introduction of improved pastures and the implementation of SPS in degraded pastures are feasible alternatives for carbon sequestration. Prior to this, Ibrahim et al. [126], in a study in Colombia, Costa Rica, and Nicaragua, have documented the carbon storage potential of SPS in soil and biomass in rangelands.This study assesses the economic-environmental benefits of implementing two different SPS in Colombia, namely (i) Urochloa brizantha cv. Toledo + Leucaena leucocephala trees (SPS Toledo) and (ii) Urochloa hybrid cv. Cayman + Leucaena leucocephala trees (SPS Cayman). These SPS are contrasted with the establishment of two monoculture grass systems commonly present in the country, namely (iii) Urochloa brizantha cv. Toledo (M Toledo) and (iv) Urochloa hybrid cv. Cayman (M Cayman).Leucaena leucocephala is a shrub legume species that attains a height of 7-18 m as it matures. It possesses a remarkable ability to regenerate vigorously after browsing or substantial pruning. Its branches display a notable degree of flexibility without succumbing to tearing. Additionally, it is highly palatable, and its capacity to fix atmospheric nitrogen is beneficial for the growth of associated grasses. This species thrives across a wide range of elevations, from sea level up to 1,600 m above sea level. It flourishes in areas with annual precipitation ranging between 500 and 3,000 mm, distributed either in a unimodal or bimodal pattern. Furthermore, it can withstand temperatures spanning from 25 to 30 • C. While it exhibits considerable drought tolerance, Leucaena leucocephala does not fare well in shaded environments. Adequate sunlight, ranging from 800 to 1,500 h per year, is essential for its optimal growth. The species is adaptable to neutral or alkaline soils and can thrive in stony terrains. However, it does not tolerate waterlogged conditions, acidic soils with high aluminum ion saturation, or intense and prolonged frosts below − 4 • C [127,128].Urochloa brizantha cv. Toledo stands as a perennial grass, originating directly from the Urochloa brizantha CIAT 26110 accession. It showcases adaptability across a broad range of climates, thriving in sub-humid tropical conditions with dry intervals lasting 5-6 months and an average annual rainfall of 1,600 mm. Similarly, it flourishes in humid tropical environments receiving rainfall exceeding 3,500 mm/year. While it can adjust to acidic soils of limited fertility, its optimal growth occurs in soils of moderate to high fertility. This grass is susceptible to infestations by cercopids and spittlebugs within pastures. Establishing it can be accomplished through either seeding or vegetative propagation. Depending on the chosen method (broadcast or furrow) and seed quality, a sowing density of 3-4 kg/ha is recommended. During rainy periods, this grass can sustain a variable animal stocking rate ranging from 2.5 to 3 Tropical Livestock Units (TLU) per hectare. Its growth potential is also applicable to cut-and-carry systems, as it can reach a height of 1.60 m. Dry matter (DM) production averages fluctuate, measuring 25.2 t/ha in the dry season and 33.2 t/ha during the rainy season. It is feasible to conduct cutting every 8 weeks [129].Urochloa hybrid cv. Cayman emerged as an interspecific hybrid originating from collaborative efforts between the International Center for Tropical Agriculture (CIAT) (breeding), the Centro de Investigación de Pastos Tropicales (CIPAT) (evaluation and selection), Grupo Papalotla (introduction, commercialization). This grass variety exhibits a remarkable ability to thrive in moist environments and displays a notable adaptability to soils predisposed to waterlogging. Its growth pattern is characterized by a decumbent form, generating an abundance of runners. Particularly noteworthy is its responsiveness to humidity, which triggers a transformation in growth behavior. In its early stages, Cayman develops decumbent stems, fostering the emergence of shoots and roots at the nodes. This unique adaptation enhances nutrient absorption and oxygen production, particularly beneficial in poorly drained conditions. Beyond its waterlogging resistance, this hybrid boasts drought tolerance, marked palatability, stoloniferous growth, and an innate resilience against pests and diseases. Moreover, it facilitates the sustenance of higher animal stocking rates, making it an asset for cattle management [130].The data used for the financial analysis were obtained from a field trial established at the CIAT campus in Palmira, Colombia. According to Holdridge [131] the study area can be classified as pre-montane wet forest (bh-P), which is located at an elevation of approximately 1,000 m.a.s.l. The annual precipitation is 1,045 mm and the rainfall regime is bimodal (March to April, October to November). The average temperature is 23.8 • C and the relative humidity 75%. The trial was established on a fertile, clayey Mollisol (40-60% clay content) [132] with a pH of 7.54, CEC of 16.4 cmol/kg, good drainage, and P, Ca, Mg, and K concentrations of 25 ppm, 7.87, 6.17, and 0.82 cmol/kg, respectively.Four treatments were established, namely (i) SPS Toledo, (ii) SPS Cayman, (iii) M Toledo, and (iv) M Cayman. Measurements were made for one year, between April 20, 2021 and April 20, 2022, to cover all four seasons occurring at the location (two rainy and two dry seasons). The overall aim of the trial was the evaluation of animal liveweight gains in two different scenarios, SPS and grass monocultures. Each of the four treatments was carried out in an area of 1 ha, which were divided into 3 plots (0.33 ha each) for rotational grazing. In the two SPS treatments, the proportion between the respective grass and the Leucaena leucocephala trees was 70/ 30, and the tree density 2,000 trees per ha (25% for shade, 75% for browsing). A total of 14 Brangus cattle were utilized for grazing, evenly distributed across the two scenarios (SPS and M) in a 50% ratio. The average age upon entry of the animals was 18.6 months, with a mean of 19.1 months for the M treatment and 18.1 months for the SPS treatment. At the commencement of the trial, the typical weight of the animals in the M treatment stood at 349 kg, while in the SPS treatment, it was 345 kg. The grazing and rest intervals were maintained at 12 and 45 days, respectively. In terms of stocking rate, the M treatment accommodated 3 animals per hectare, whereas the SPS treatment hosted 4 animals per hectare.Regarding the environmental evaluation, following a comprehensive review of available information and data, one notable environmental benefit and a single ecosystem service were considered, namely (i) the mitigation of CH 4 emissions and (ii) microclimatic regulation. For the analysis of CH 4 emissions reductions, the study by Gaviria-Uribe et al. [133] was considered. Their research provided estimates of CH 4 emissions within this experiment across various diets in both M and SPS scenarios. As for microclimatic regulation, an assessment was conducted by measuring the extent of shaded areas within the SPS treatments on September 1, 2022, utilizing the Fields Area Measure application.In this study, a discounted cash flow model was employed to estimate profitability indicators, facilitating a comparative analysis of various investment options (SPS, M). The assessed indicators encompass NPV, IRR, and B/C, following the guiding principles outlined by Park [134]. These indicators are derived by adopting the most probable values within distinct specified models, aligning with the benefits and costs associated with each investment alternative. The assessment is constructed through a juxtaposition of the calculated profitability indicators for the different treatment scenarios. Costs incurred were categorized into four main areas: (i) establishment and maintenance costs, (ii) opportunity costs, (iii) operational expenses linked to animal health and supplementation, and (iv) labor costs. Conversely, benefits stem from the cattle's commercialization, with their profitability parameters contingent upon the liveweight gain observed in each of the evaluated treatments.For the elaboration of the cash flows, the following technical and economic assumptions were made: Technical Assumptions: All cattle engaged in the treatments were exclusively of the Brangus breed. To formulate the cash flows, stocking rates of 3.0 and 4.0 animals per hectare were adopted for the M and SPS treatments, respectively. These rates remained consistent throughout the analysis period. Liveweight gain was meticulously assessed for all treatments at both the commencement and conclusion of the experiment (Table 1). The feeding duration for the animals was standardized at 365 days across all treatments.Evaluation horizon: The evaluation horizon was determined based on the typical lifespan of the grass technologies, which is commonly recognized as 10 years [135], spanning from 2020 to 2029. It is worth noting, however, that both evaluated grass varieties can potentially remain productive for a considerably longer duration with proper management practices such as grazing and fertilization. Additionally, the assessed Leucaena leucocephala trees generally exhibit lifespans extending beyond 10 years. It is also important to acknowledge that in practical scenarios, farmers frequently exhibit reluctance to renew pastures, resulting in the continued utilization of established technologies for over 10 years. Nonetheless, this often leads to a decline in pasture productivity due to the scarcity of adequate pasture management practices. Nevertheless, to promote the effective adoption and utilization of such technologies, the present article adopts a 10-year timeframe as the technology lifespan.Discount rates: The determination of financing costs was based on the established credit lines for cattle production systems, including those incorporating silvo-pastoral settings, as defined by Finagro, the Colombian Fund for Financing the Agricultural Sector [136]. This discount rate is commonly employed as the representative opportunity cost for capital investments and is closely tied to the risk factors inherent in agricultural endeavors. To encompass the potential impact of variations in discount rates on system profitability, multiple scenarios were explored using DTF (fixed-term deposit rate) + 3%, 5%, and 8%, respectively. The projections for the discount rate were derived from two primary sources: (i) the Bancolombia Annual Report of Economic Projections for Colombia in 2020 [137], and (ii) the macroeconomic projections report for 2020 from the Banco de la República of Colombia, which collates insights from both local and foreign analysts [138].Permanent labor: Following the labor weighting factors outlined by Fedegan [139], a cattle fattening-oriented system requires approximately 2.3 permanent positions for every 100 animals. The salary estimate is calculated based on the prevailing monthly legal minimum wage (MW) for the year 2019, encompassing transportation assistance, social security contributions, and associated benefits, totaling around US$400 per month. To forecast the evolution of the minimum wage throughout the analysis period, it was assumed that the variation would mirror the anticipated inflation rate for each year, coupled with a labor productivity adjustment set at 1%, in accordance with historical data from national statistics [140]. The labor remuneration used in this analysis aligns with Colombia's salary dynamics, which are anchored by the MW. As reported by DANE [141], approximately 32.2% of the employed population in Colombia received a salary near 0.9 MW in 2020, while 17.8% fell within the range of 0.9-1.1 MW. This nuanced distribution provides insight into how rural area wages correspond to national average wage trends. Considering its proximity to the national average, with minor regional variations, the adopted MW serves as a suitable proxy.Inflation: Incorporating the impact of inflation, the analysis encompassed the estimation of revenues and costs over the evaluation period. Projections for income were based on the Consumer Price Index (IPC) data published by the Banco de la República of Colombia [142]. In parallel, the Producer Price Index (IPP), as calculated by the National Administrative Statistics Department of Colombia (DANE) [143], was employed to gauge production costs.Cattle price: In the context of local weather events and the global impact of the COVID-19 pandemic, the Colombian cattle sector experienced inflationary and external influences, leading to a notable rise in cattle prices [1,42]. Given the exceptional nature of these price fluctuations, the study referred to prices from 2019 for the purposes of analysis.Risk is defined as the potential that the actual investment return will fall short of the expected investment return [134]. Consequently, profitability is intertwined with the volatility of income and cost streams, hinging on the unpredictability of key variables within the investment project, such as yields and market prices. Rural investment endeavors are particularly exposed to unique risks, their outcomes being contingent upon a broad array of variables, many of which lie beyond the investor/producer's control, such as climatic factors. In this context, it becomes imperative to integrate risk considerations into the assessment of profitability indicators for each evaluated investment. To this end, a Monte Carlo simulation model was employed. Monte Carlo simulation involves generating a sample of outcomes based on specified probability distributions [134]. This approach empowers decision-makers to explore potential outcomes and gauge the influence of risk on investment project profitability indicators. Executing the simulation necessitates the identification of random input variables, those capable of adopting multiple feasible values, and defining the plausible range for each. Probability distributions are then assigned to these variables, followed by the computation of projected indicator profitability. In the present study, the Monte Carlo simulation was executed using the @Risk software package (Palisade Corporation), involving 5,000 iterations and a confidence level of 95% across all treatments.The decision criteria are rooted in the average values of the profitability indicators NPV and IRR, alongside the Benefit-Cost Ratio (B/C), which stem from the simulations conducted for both the M and SPS treatments.Net Present Value: This indicator represents the present value of the cash flow stream, calculated as the aggregate of discounted benefits subtracted from discounted costs [144].Internal Rate of Return: As outlined by Gittinger [144], an alternative approach to assess the value of a project using the revised cash flow involves identifying the discount rate that renders the net value of the cash flow equivalent to zero. This discount rate is referred to as the Internal Rate of Return (IRR), which, in essence, signifies the average return on the investment expenditure throughout the entirety of the project's lifespan. The equations for the NPV (Equation ( 1)) and IRR (Equation ( 2)) are as follows:(1)where E(FC t ) is the expected value of the net profit flow for period t, Var(FC t ) is the net profit flow variance for period t, r the real discount rate, r* the internal rate of return, and t the evaluation horizon of the project.Cost-benefit analysis: Cost-benefit analysis (CBA) is a structured economic evaluation tool that involves a comparison between the costs and benefits of a given project. Functioning as a comprehensive analytical framework, CBA serves as a means to assess and appraise both public and private ventures [145]. Economically speaking, the Benefit-Cost Ratio (B/C) applies a discount mechanism to both the influx of benefits and the outflow of costs, utilizing a rate that approximates the opportunity cost of capital. This approach establishes a quantifiable linkage between the discounted value of project benefits and the corresponding value of the discounted costs associated with it. The equation for the B/C (Equation ( 3)) is as follows:where Bn are the benefits in period t, Cn the costs in period t, r the real discount rate, and t the evaluation horizon of the project. The Benefit-Cost Ratio (B/C) provides a rough indication of the effectiveness of funds allocated to an investment, offering a transparent gauge of the viability and allure of an investment project. The decision criteria can be summarized as follows:• B/C > 1: This suggests that the revenue generated by the investment will surpass all associated expenditures, indicating project profitability. • B/C = 1: This signifies that both the generated revenue and expenses of the investment will be equivalent. The decision to proceed with the investment remains neutral. • B/C < 1: This implies that the generated revenue from the investment will fall short of covering all incurred expenses, indicating project unprofitability.The environmental evaluation encompasses the economic appraisal of environmental advantages and ecosystem services arising from the SPS as opposed to the M treatments. In terms of environmental benefits, the replacement of traditional M systems with SPS leads to a reduction in CH 4 emissions. In the realm of ecosystem services, three prominent aspects have been identified: (i) microclimatic regulation achieved through the shading effect of trees, (ii) carbon storage and sequestration within tree biomass, and (iii) soil nitrogen fixation. However, for the scope of this study, the focus is on valuing the environmental benefit of CH 4 emission reduction and the ecosystem service of microclimatic regulation. This decision is due to the absence of available technical ecological measurements for the assessment of carbon storage and capture, as well as soil nitrogen fixation.To assess the value of CH 4 emission reduction, the market price method [146] was implemented, leveraging data from major global Tradable Emission Permit Systems and economic mechanisms like CO 2 taxes. The market price method establishes the economic worth of CO 2eq. (v) by multiplying the volume of avoided CO 2eq. emissions (q) with the average market price for emissions and/or CO 2 taxes as sourced for the analysis year (p) (as represented in Equation ( 4)). In order to ascertain q, this study draws upon findings from Gaviria-Uribe et al. [133], who indicated that for every gram of animal liveweight gain, emissions of 0.36 and 0.33 g of CH 4 occur in the M Toledo and SPS Cayman treatments, respectively.To appraise the reduction in CH 4 emissions, certain assumptions were made regarding the initial (220 kg) and slaughter weight (450 kg) of the cattle. These assumptions enabled the utilization of daily liveweight gain data from different treatments and the CH 4 emissions factor per gram of liveweight gain. This information was employed to estimate emissions generated up until the point of the animal's sale. Since Gaviria-Uribe et al. [133] exclusively provide data for M Toledo and SPS Cayman, it was deduced that CH 4 emissions for SPS Toledo would be akin to those of SPS Cayman. Consequently, identical values as those for SPS Cayman were employed for assessing the monetary worth of CH 4 emissions reduction in SPS Toledo.To assess the monetary value of the microclimatic regulation ecosystem service, the method of avoided costs [146] was employed. This approach involves estimating the expenses associated with the establishment and upkeeping of a gray infrastructure that mirrors the shade coverage provided by the trees within the SPS. The chosen gray infrastructure for comparison is a shade mesh, as it is the prevalent choice for cattle systems in Colombia. In this context, the monetary value of the microclimatic regulation ecosystem service (v) is determined by multiplying the area encompassed by the shade (q) with the average costs associated with installing and maintaining the selected gray infrastructure (c), as illustrated in Equation (5).(5)It is essential to acknowledge several limitations that should be taken into account when interpreting the findings presented in this financial analysis.Firstly, due to the age of the cattle upon entering the SPS treatments, it was not feasible to measure initial-stage liveweight gain, a crucial component of the productivity curves associated with grazing. While the outcomes for the SPS treatments were more favorable compared to the M treatments, the exclusion of initial-stage liveweight gain measurements could potentially impact the results of the SPS treatments.Secondly, both input and labor costs used in modeling the cash flows for cost, benefit, and other indicator measurements were influenced by external factors, such as the COVID-19 pandemic [42,147]. The pandemic led to disruptions in agro-industrial input supply chains and local price inflation, directly affecting the implementation costs of the studied treatments. Consequently, the results of this study are significantly shaped by the global and local macroeconomic context that unfolded since 2020. This economic environment also impacted on the projections incorporated into the cash flow analysis. Considering the economic uncertainty at both national and international levels, inflation, wage, and producer price index forecasts used to project benefits and costs demonstrated higher-than-expected increases. Despite consulting multiple sources to compare forecasts from both public and private institutions, the prevailing trend was towards upward adjustments due to the economic uncertainty of 2020. To mitigate these extraordinary effects in the analysis, 2019 prices were adopted as the foundation for this study.Furthermore, it is important to recognize that this analysis was conducted on an experimental research scale within CIAT's campus.Consequently, the estimated productivity and costs may deviate from scenarios on farmers' fields under commercial conditions. In the realm of environmental evaluation, it is important to highlight that SPS generate various other environmental benefits and ecosystem services that were not quantified in this study. These include carbon storage and sequestration in tree biomass, soil nitrogen fixation, fertilization of soil through animal excrement, and the creation of habitats for various species. These services were not valued due to the lack of ecological estimates of physical units for each service specific to the SPS setups of interest. Furthermore, a social valuation exercise was not undertaken as it would have necessitated an additional experimental design involving participation from producers to ascertain values based on their perceptions. Additionally, the avoided cost method used reference prices from the Colombian Cauca Valley department, where the trial was located, which may yield different outcomes if applied to other regions within Colombia.One of the most important basic aspects for the financial analysis of SPS and M is the cost structure associated with the setup and management of the technologies, given that these largely define the investment decision producers need to make when adopting and implementing a new feed production system. As can be observed in Table 1, higher costs occur for the establishment of SPS, particularly they increase by 26% and 3% for SPS Cayman and SPS Toledo, respectively, when compared with the M alternatives. Regarding the maintenance and renovation costs, they tend to be slightly lower for the SPS, since less area is sown with the more labor and fertilizer intensive grasses which also need partial renovation after around five years to maintain pasture productivity stable. Regarding the benefits, due to the increased amount and quality of available feed, the animal stocking rates are 33% higher in SPS than in M, which translates into higher daily liveweight gains (+51%), annual per hectare animal productivity (+49%), and annual income from the sale of meat (+34%) in SPS.Table 2 presents the results for the financial indicators for the M and SPS treatments. A negative NPV was obtained for the two M treatments, precisely of US$-268 and US$-527 for M Toledo and M Cayman, respectively. Both treatments also show a negative IRR, although for M Cayman it is close to zero, and a high risk of obtaining economic loss, 67% and 81% for M Toledo and M Cayman, respectively. The two SPS treatments, on the other hand, show disparate results. As for the NPV, SPS Toledo presents a positive value of US$35, whereas it is negative for SPS Cayman (US$-218), which is related to the significant difference in establishment (+69%), maintenance (+30%), and renovation (+30) costs of the pasture in SPS Cayman compared to SPS Toledo. Likewise, the IRR is positive for SPS Toledo but negative for SPS Cayman, and the risk of obtaining economic loss is high for both. The B/C ratio is slightly positive for the SPS treatments. Despite these rather discouraging numbers, the data show that significant improvements occur in all indicators when implementing SPS instead of M.Figs. 1 and 2 show the results of the Monte Carlo simulation risk analysis for the M and SPS treatments. It can be observed that despite the still high risk of obtaining economic loss (NPV<0) in the SPS treatments (in 49% and 59% of the scenarios generated for SPS Toledo and SPS Cayman, respectively), it is significantly lower than in the M treatments (67.5% and 80.8% for M Toledo and M Cayman, respectively).Based on the results of Gaviria-Uribe et al. [133] it is found that the SPS Cayman treatment achieves a CH 4 emissions reduction of 0.03 g per gram of animal liveweight gain, which is equivalent to a reduction of 0.63 g of CO 2eq. compared to the M Toledo treatment. If this diet was replicated in a larger SPS, for example with 1,000 cattle, a reduction of 145 tons CO 2eq. could be achieved. Table 3 presents an overview of the data presented by Gaviria-Uribe et al. [133]. The economic value of the reduction of avoided CH 4 emissions corresponds to a potential income that a producer can access by implementing good environmental practices. This reduction contributes to the objective of climate neutrality in livestock farming, which is encouraged in Colombia by a program for payments for environmental services (PES). The PES program has a special emphasis on supporting livestock producers who implement silvo-pastoral systems and demonstrate environmental benefits such as the CH 4 emissions reduction presented in this article [119,148].When consulting the most important global carbon markets [149], the carbon tax in Colombia [150], the minimum price recommendation for carbon credits of the International Monetary Fund (IMF) [151], and the general equilibrium model with the Tradable Emission Permit System in Colombia of the National Planning Department (DNP) [152], an average price per ton of CO 2eq. of US$45.25 per ton can be defined for the period between January and August 2022 (Table 4).According to this average price, the monetary value for the environmental benefit of CH 4 emissions reduction in SPS Cayman can be estimated at US$6.12 per cattle. Considering the stocking rate of the experimental setup of four cattle per hectare, a total monetary value of US$24.49 per hectare can be observed, which is equivalent to an annual value of US$28.83. A replication of SPS Cayman at larger scales, for example in a cattle fattening system with 1,000 animals, could thus generate a monetary value for CH 4 emissions reductions of US$6,122 (Table 5).Concerning the microclimatic regulation ecosystem service, the SPS treatments encompass an area of 12,082 m 2 under shade, as determined through field measurements, translating to a shade coverage of 60.4%. Engaging with ten shadow mesh providers in the Cauca Valley Department, the average prices for shadow mesh (per m 2 ), poles (per pole), and labor (per day) were ascertained to be US $0.78, US$5.49, and US$9.24 respectively. The anticipated lifespan of the infrastructure is three years. Thus, should the tree shade coverage within the SPS treatments be replaced by shadow mesh, the total cost over three years would amount to US$12,158, equivalent to US$4,053 annually and US$2,026 per hectare per year. When extrapolated to a larger scale, such as a 1,000-ha cattle fattening system, the monetary value of microclimatic regulation could potentially reach US$2,016,414 per year (as indicated in Table 6).In the last step of analysis, the monetary value of the environmental benefit of CH 4 emissions reduction provided by the SPS was integrated into the financial analysis. Table 7 shows how the financial indicators change under this scenario compared to the Base Scenario presented in Table 2.As the data for Scenario 1 shows, when the environmental benefit of CH 4 reduction is included in the economic evaluation, all economic indicators improve for the SPS treatments. Particularly, the NPV for SPS Toledo increases by 741% compared to the SPS Toledo without CH 4 reduction, and for SPS Cayman, it turns positive for the first time. Likewise, for SPS Toledo, the IRR increases by 517% and further improves for SPS Cayman. The B/C, however, only slightly increases when including CH 4 reduction. Regarding the risk of obtaining economic loss (NPV<0), further improvements can be observed for both SPS when CH 4 reduction is considered (Fig. 3). Nevertheless, the risk of obtaining economic loss remains high for both SPS Toledo (39.2%) and SPS Cayman (50.2%). This study evaluated the viability of integrating the tree legume species Leucaena leucocephala in two different grass monoculture cattle feeding systems, namely Urochloa brizantha cv. Toledo and Urochloa hybrid cv. Cayman, under two SPS arrangements. Particularly, financial viability of this endeavor was evaluated in two scenarios, namely (i) a base scenario, which only considered the productive parameters, and (ii) a scenario which considered productive parameters and the environmental benefit of CH 4 emissions reductions in the SPS.The findings of the study reveal that the introduction of SPS in the base scenario yields noticeable enhancements in profitability indicators, such as NPV, IRR, B/C, and NPV <0, regardless of the chosen grass technology. However, despite these improvements, only the SPS Toledo arrangement displays a positive NPV and IRR. It is worth noting that even this most favorable option does not exhibit strong attractiveness due to the relatively modest values of these indicators. These results exhibit some alignment with existing literature, as many studies exploring the economic viability of SPS similarly report significant enhancements, akin to the current study. Nonetheless, in most cases, these enhancements typically translate into highly positive economic indicators and overall viability [57,[96][97][98][99]95]. The unfavorable indicators observed for SPS Cayman can be primarily attributed to its comparatively higher establishment costs in contrast to SPS Toledo. Both cases, however, may also be influenced by relatively low daily liveweight gains observed in the trial that underpins this evaluation. These observed gains average less than 240 g in both SPS, which contrasts with similar SPS setups where gains typically range between 600 and 700 g [57]. These differences could contribute to the relatively discouraging results observed in this particular study. This rather unfavorable investment panorama, however, further changes when the monetary value of the environmental benefit of CH 4 emissions reductions is integrated into the profitability analysis. By integrating the value of CH 4 emissions reductions, which was estimated at > US$6, >US$24, and >US$28 per cattle, hectare, and year, respectively, the NPV of SPS Toledo increases by >700% and the one of SPS Cayman turns positive. Likewise, the other indicators further improve for both SPS. Nevertheless, this benefit alone still is not enough to make both SPS an attractive investment alternative as the relatively low IRR shows. The estimated savings in CO 2eq. emissions of 9% in the SPS, however, are attractive when it comes to climate change mitigation. Particularly, a SPS Toledo or SPS Cayman system of 1,000 animals (which would translate into an area of 250 ha considering the stocking rate of four animals per hectare) can save 145 tons of CO 2eq. compared with the grass monocultures, which are rather common in the Colombian cattle landscape [55,153]. This coincides with the study of Cuevas-Reyes et al. [94] who found that the profitability indicators of a SPS with Leucaena leucocephala and Cynodon dactylon significantly improve when their carbon capture potential is considered and monetized. Likewise, it coincides with Bussoni et al. [93], whose findings suggest that the reduction of CO 2eq. emissions can be combined with the productive goals of cattle farming under SPS.The ecosystem service of microclimatic regulation has been estimated to hold an economic value of $2,026 per hectare per year. It is important to note, however, that this value is not factored into the financial analysis. The internalization of the shade effect from trees serves as an intermediate component that does not impact the discounted NPV of SPS. This shade effect is utilized for the cattle's own intermediate consumption within the SPS. The establishment of the evaluated SPS, which yields approximately 60% shade coverage, brings about notable benefits. These advantages contribute significantly to various aspects, including (i) animal welfare improvement: SPS mitigate heat stress and reduce the presence of hematophagous flies, enhancing the well-being of the cattle [154][155][156][157][158], (ii) environmental enhancement: The shade cover of SPS reduces water consumption by the animals and promotes biodiversity [159][160][161], and (iii) productivity and quality enhancement: SPS positively influence productivity and quality indicators [154,159,162]. This multi-faceted impact highlights SPS's potential to contribute to climate change mitigation by curbing CH 4 emissions [110][111][112][113]. Additionally, SPS aid in climate adaptation by providing thermal comfort to animals amidst rising temperatures and bolstering feed availability during critical periods [158,159,163,164]. While not directly incorporated into the financial analysis, these ecosystem services reinforce the overall value and resilience of SPS systems.To fully unlock the comprehensive environmental, economic, and productive benefits of SPS, a conducive environment for adoption must be fostered. This involves tailoring strategies to account for the distinct characteristics and disparities among adopting farmers in various regions, as well as acknowledging their unique needs, experiences, and local expertise. However, creating such an adoption-friendly environment proves to be a formidable challenge, particularly in countries like Colombia where SPS adoption rates remain generally low [39,57,65,66]. The adoption process itself encounters numerous barriers that necessitate diligent efforts to overcome, as evidenced by research findings. These barriers encompass a spectrum of challenges, ranging from financial hurdles (lack of access to credit, extended payback periods) to knowledge gaps (limited access to information, technical guidance, and extension services; intricacies of SPS practices) [65,66,73,[165][166][167]. Socio-cultural factors also play a role, as traditional norms often associate pastures with tree-free landscapes [65]. Labor considerations add another layer of complexity, with SPS demanding higher labor input and facing competition from other (sometimes illegal) sectors [168]. Land tenure issues can further hinder adoption [72,73], while environmental concerns (perceived or actual) and the adaptation of species to specific environments pose additional challenges [169]. Market dynamics, legislative constraints, and a general farmer aversion to risk contribute to the array of barriers [39,65]. Addressing these multifaceted barriers requires a holistic and nuanced approach that combines targeted interventions, robust policies, and comprehensive support mechanisms. By strategically navigating these challenges, countries can effectively elevate SPS adoption rates, harness the array of benefits they offer, and ultimately establish a more sustainable and resilient agricultural landscape.To promote the widespread adoption of SPS, a variety of instruments and incentives have been proposed and implemented through research and policy initiatives. Among these, Payments for Ecosystem Services (PES) have emerged as a notable strategy, offering strong incentives for adoption, particularly when farmers are actively engaged in their design [53,165,168,169]. Additional incentives encompass credits aimed at establishing SPS, tax benefits, commercial incentives such as price premiums, development of effective extension systems and technical support, removal of regulatory hurdles, and subsidies for trees, seeds, inputs, and equipment [39,165,170]. Research has also highlighted the importance of bolstering social networks and social capital, indicating their significant role in driving SPS adoption [66,72]. In instances where rural extension services are limited, complementary support from research initiatives can play a pivotal role in boosting adoption rates [166]. Over the past decade, Colombia has made considerable strides in scaling up SPS adoption, particularly by generating incentives to foster widespread implementation. Notably, the Sustainable Colombian Cattle Project (2010-2019) has facilitated the adoption of approximately 5,000 ha of SPS across the country [42]. The establishment of the Sustainable Cattle Roundtable in 2014 further supports the development of public policies and capacity-building efforts in sustainable cattle farming, with a particular emphasis on SPS [43,171]. This collective effort has culminated in the release of Colombia's first national-level public policy on sustainable cattle in 2022 [172]. Other significant policy advancements include the establishment of Zero-Deforestation Agreements for Beef and Dairy in 2018 [173], the development of Nationally Appropriate Mitigation Actions for cattle since 2014 [174], and the enactment of the SNIA law reforming the national agricultural innovation system in 2017 [175]. In terms of financing, a groundbreaking credit line dedicated to SPS was introduced in 2020, aimed at supporting the acquisition of planting materials [176]. Furthermore, the private sector is actively contributing to incentives, such as product differentiation and price premiums (e.g., Sustainable Cattle Initiative GANSO, AngusAzul's sustainability program), and offering technical assistance for SPS establishment (e.g., GANSO, Alquería's Heirs of Tradition program) [40,42,177,19].While the benefits of scaling SPS are substantial, it's crucial to also acknowledge the potential unintended consequences of widespread adoption. Parodi et al. [178], for instance, advocate for implementing SPS primarily in areas unfit for crop production to mitigate unwanted competition with other agricultural systems. However, this approach may introduce negative outcomes, as evidenced by increasing deforestation trends observed when cattle intensification takes place on marginal lands [179,180], particularly when land tenure is unclear [181]. Castro-Nuñez et al. [8] highlight another potential concern, noting that the establishment of SPS can have adverse effects on forest cover in Colombia. This is attributed to improved cattle birth rates within SPS, which in turn lead to surplus calves often being sold to unsustainable fattening farms situated at the deforestation frontier. Notably, cattle farming expansion ranks among the primary drivers of deforestation in Colombia and Latin America [2][3][4][5][6][7][8][9][10][11][12]. Moreover, the productivity gains yielded by SPS could inadvertently encourage cattle farmers to further expand their operations at the expense of forests and other vital ecosystems [68], a phenomenon referred to as the Jevons paradox [182,183]. To counteract such negative and undesired consequences and to uphold the principles of sustainability in cattle farming via SPS, a strategic combination of the aforementioned incentives and robust monitoring and control mechanisms becomes imperative [68,[184][185][186]. These mechanisms may include deforestation monitoring, traceability systems, and taxes targeting conventional pasture usage, among others. By implementing such measures, the potential adverse effects of SPS expansion can be mitigated, ensuring that the promise of sustainability in cattle farming is upheld.Silvo-pastoral systems are considered a sustainable production alternative for cattle systems in the global tropics and especially in Latin America. However, little is known yet about the financial viability of establishing such systems in different contexts and the added economic value of the environmental benefits and ecosystem services they provide. The present study aimed at contributing to closing this research gap by providing an economic-environmental analysis of two SPS for the Colombian context, considering the environmental benefit of methane emissions reductions and the ecosystem service of microclimatic regulation.The study shows that when the analyzed traditional grass monocultures are transformed into the proposed SPS, the profitability indicators get significantly better, yet no outstanding financial viability was observed in this case. This, however, changes once the monetary value of CH 4 emissions reductions is considered in the financial analysis, converting the proposed SPS into more attractive investment options. A widespread adoption of these SPS can create important socio-environmental benefits, such as the reduction of 145 tons of CO 2eq. for every 1,000 cattle fed in these systems, valued at US$6,121 -roughly what 32 typical passenger vehicles emit per year [187]. Likewise, the suggested tree cover in the SPS generates 60% tree shade, which, per 1,000 ha creates a value of US $>2million and adds to the numerous other ecosystem services SPS provide.However, for the scaling of SPS, numerous adoption barriers must be overcome, for which in Colombia, several public and private initiatives and programs were already established. In this regard, it is important that these endeavors consider the particularities and differences among the adopting farmers in different regions, as well as their needs, experiences, and local knowledge. It is also essential to integrate the monetary values of the environmental benefits and ecosystem services into the financial analysis of SPS, so that the stakeholders involved in the adoption process can make more informed decisions. This is, however, not always possible since technical data is not available for all environmental benefits and ecosystem services SPS offer for which it is recommended to include this in future research. To avoid unintended negative consequences in the adoption of SPS, such as deforestation, incentives (e.g., PES, credits, price premiums) need to be coupled with monitoring and control mechanisms (e.g., traceability, zero-deforestation). Only then, the sustainability claims of SPS can be upheld. Likewise, the knowledge component of SPS is very complex, and much of the adoption depends on the available knowledge, extension, and technical assistance, as well as farmer networks. A strengthening of these elements and better coordination among the actors involved is thus recommended. funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.","tokenCount":"8222"} \ No newline at end of file diff --git a/data/part_6/0212490421.json b/data/part_6/0212490421.json new file mode 100644 index 0000000000000000000000000000000000000000..a7bb4d277d44395d20e3208ba33f2e53cd8d41ee --- /dev/null +++ b/data/part_6/0212490421.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"cfe9159b17d6372e042f6295488771c7","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/d26d2420-1110-4fba-9ef9-931fbb22a834/retrieve","id":"-1358454903"},"keywords":[],"sieverID":"187c1d01-2407-481a-b3a5-167bd88fb08b","pagecount":"8","content":"What is seed treatment? Benefits of Seed Treatment:S eeds saved by farmers may be infected with microbes that can induce diseases on the seedling and the crop. This can affect seed germination and may be transmitted from seed to seedling to plant.Seed treatment prevents and controls seed-, soil-, and air-borne diseases. It improves germination, vigor, and productivity.Seed treatment or dressing refers to the application of biological, physical, and chemical agents and techniques to seed to provide protection and improve the establishment of healthy crops.Seed treatment may involve use of fungicide, insecticide, or a combination of both, applied to the seeds to disinfect them from seed-borne or soil-borne pathogenic organisms and storage insects.Seed treatment is like immunization -it provides a good insurance and protection of your seed and crop against diseases, seed-borne or soil-borne pathogenic organisms and storage insects. This involves application of a chemical (fungicide/insecticide) to seed prior to planting to provide effective protection against many seed and soil-borne plant pathogens. The chemical treatment guards against the various seed rots and seedling blights that occur during storage or after planting. Always wear protective equipment and follow appropriate safety procedures when using chemical seed treatment products.Stem-feeders (Aphids)How to do seed treatment Procedure for chemical seed treatment Seed treatment materials are usually applied to seed in various forms: dust; slurry (a mixture of wettable powder in water) and liquids. Slurry application is the most common method especially for smallholder farmers. Read and follow instruction for the specific application procedure for each seed treatment product.Always wear protective equipment and follow appropriate safety procedures during seed treatment.Biological seed treatment consists of active ingredients that can include microbes such as fungi and bacteria, as well as plant extracts and algae extracts. The biological agents improve health of the root zone by competing with pathogens that may colonise seeds and newly emerged roots thus protecting the germinating seed and seedling from infection. The microbes stimulate rapid root development and nutrient uptake by the growing crop. Some of the biological agents for seed dressing include Trichoderma spp., Rhizobium spp., Bacillus subtilis, Psedomonas flourescens, Azospirillum spp., Neem and sea weed extracts Examples of biological seed dressing products in Kenya include: TriCoat, Mazao Flourish, Trianum, Biofix and LEGUMEFiX.Biological seed treatment products are environmentally friendly and easy to apply. These however required proper storage to keep viability of the biological agents.Plan to treat your seeds at the start of the rains when soil is moist and ready to plant. Measure the desired amount of seed using a tin (gorogoro), bucket or a weighing balance. You can treat both own saved seed or certified seed.Dissolve recommended amount of fungicide/insecticide per kg seed in water inside a plastic bag or bucket to make a slurry.Weka dawa kwenye ndoo kisha ongeze maji kidogo. Changaya dawa na maji ili iwe chepechepe.Put the seed in a bucket or a plastic bag. Do not use anything used for cooking or etching water.Weka mbegu kwenye ndoo au karatais ya plastiki. Usitumie chombo cha kupikia au kuteka maji.Stir and mix well with a stick or hand protected by the glove. Alternatively seal the bucket and shake vigorously to coat seed uniformly with fungicide slurry.Pour out the seed on a sack under a shade for product to stick on seed. Keep the sack away from rain or direct sunlight Mwaga mbegu juu ya gunia chini ya kivuli ili dawa ikaukie kwenye mbegu. Weka mbali na mvua au jua kali.","tokenCount":"570"} \ No newline at end of file diff --git a/data/part_6/0218144928.json b/data/part_6/0218144928.json new file mode 100644 index 0000000000000000000000000000000000000000..b05cd9a2211e5f47783c920588c87b662dd8fe8c --- /dev/null +++ b/data/part_6/0218144928.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"50e2f2493f288c357af9e2a9e944948f","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/b12eacf0-57ed-4ba1-8515-b5c121c8d23c/retrieve","id":"-2087490110"},"keywords":["P740 -Product Line 5.1.2: Fit-for-purpose national and meso-level strategies for livestock investment and policy Geographic scope:","National Country(ies):","Tanzania, United Republic"],"sieverID":"b43343c6-a506-47c9-804f-d17a3beee321","pagecount":"1","content":"Upgrading the smallholder dairy value chain: A system dynamics ex-ante impact assessment in Kilosa District, Tanzania Commissioning Study: ILRI Part II: CGIAR system level reporting Links to the Strategic Results Framework: Sub-IDOs: • Increased livelihood opportunities Is this OICR linked to some SRF 2022/2030 target?: No Description of activity / study: The assessment looked at the ex-ante impacts of two policy interventions that improve productivity of local-breed cows, artificial insemination (AI) and producers' access to distant markets through a dairy market hub. The policy interventions were introduced under the CRP Livestock and Fish.","tokenCount":"93"} \ No newline at end of file diff --git a/data/part_6/0257639183.json b/data/part_6/0257639183.json new file mode 100644 index 0000000000000000000000000000000000000000..6c72c99bc1b8362e54d5adfd3d9016bc21ed36a2 --- /dev/null +++ b/data/part_6/0257639183.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"df5d23f1ea991943730493ad046c8352","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/f61708be-d44f-44c6-a7cc-84f9582f9cf5/retrieve","id":"1869637284"},"keywords":[],"sieverID":"afe90c00-893c-4212-860f-312ebcdc09c7","pagecount":"16","content":"Essential oils Distilling knowledge, re)discovering plants Field report from Haiti FOCUs ,11 Breadfruit Nutritious and healthy, but badly under-utilised viEwpOiNT ,16 An agricultural renaissance By Arlington Chesney iN BRiEF ,3 pUBLiCATiONs ,12 BETwEEN Us ,15 Family farming The future of the world After a long period of neglect and impoverishment caused by economic changes, small-scale farmers are at last back at the heart of the development debate. improving their livelihoods and their conditions for production is essential for countries in the south.It is an oft forgotten fact: 2.5 billion men and women -almost 40% of the world population -depend on agriculture for their livelihoods. In order to feed themselves and earn a living, they rely on the land, the sky and…the considerations accorded to them by governments and the international community. Sidelined in recent decades in favour of the agribusiness sector, these small-scale farmers are sinking deeper and deeper into poverty, especially in sub-Saharan Africa: they now account for three-quarters of the world's poor. \"Will the African farmer be simply wiped off the map?\" ponders SOS Faim in its 2007 campaign.Militants are not the only ones sounding the alarm. \"Only small-scale farmers can put an end to famine in Africa,\" declares the International Food Policy Research Institute (IFPRI). The World Bank shares the same view. In its World Development Report 2008, which focuses on agriculture for the first time since 1982, it maintains that promoting the growth of this sector is the most effective way to fight poverty.The future of family farming is well and truly back on centre stage. A common feature of these ventures is that they are family-runand managed, with production mainly aimed at satisfying the needs of the household and ensuring the sustainability of the farm. Crops, generally diversified so as to limit risks, are mostly sold at local markets, though in certain cases, some may go for export. Family farms are often labelled as 'traditional', or even 'archaic', decried for being unable to increase productivity or to modernise. But such stereotypical descriptions fail to take into account the diversity of the farmers and their ability to adapt.The bulk of agricultural export commodities such as coffee, cocoa and cotton are produced by family farming systems. The expansion of rice, horticultural and fruit production shows that small-scale producers are perfectly capable of innovating and increasing output if they are given the means. In all ACP countries, family farms play a vital role in producing food and supplying the towns. They continue to be an important source of support for urban families facing difficulties. But in spite of this, donors and governments have in the past few decades remained steadfast in their belief that large-scale agro export farms, deemed to be 'dynamic' and 'competitive', represent the only real hope for modernising agriculture.In developing countries, the withdrawal of the State from agricultural training and support, coupled with the structural adjustments of the 1990s and the opening up of markets have combined to erode conditions for family farm production and have led to a deterioration in rural living condit ions. Compounding the problem is trade liberalisation, which puts farmers from very different production backgrounds in competition with each other: intensive mechanised farming is 1,000 times more productive than manual rain-fed agriculture. The concentration of various food chains in the hands of major agrifood enterprises and changes in retail distribution systems (the proliferation of supermarkets, see Spore 110) are gradually chipping away at the position of small-scale farmers who, unlike other producers, receive no support from their governments. The population growth has led to a parallel expansion in land under cultivation, sometimes on marginal soils, and a reduction of the acreage available for each farmer. In Côte d'Ivoire, for example, the proportion of land farmed rose from 8.5% to 23.5% of the total territory between 1961 and 1999.The priority given to ever growing numbers of urban consumers and the subsequent reliance on subsidised imported products have caused agricultural prices to tumble. As a result, farmers in many regions can no longer make a decent living and in certain areas cannot even produce enough food for their own households (Spore 128).In Africa, population growth, which has hovered close to 3% a year since 1960, is higher than that of agricultural output, which has remained at 2% throughout the same period. Rates of output per capita have fallen, exacerbating food insecurity and encouraging young people to join the exodus to the towns or to leave their country altogether. In southern Africa, AIDS is devastating rural labour forces and intensifying the decline.In spite of this trend, the proportion of persons who depend on agriculture or related activities for their living remains extremely high and this sector plays a major role in the labour market. That is the conclusion of RuralStruc, a World Bank research programme on the consequences of liberalisation for rural development. In Senegal, for example, a highly urbanised country, of the 11 million inhabitants, 7 million -only a fraction lower than the number in 1960 -still rely mainly on rural activities for their livelihoods, in the absence of enough jobs in the towns. Each year, 120,000 young rural dwellers enter the labour market. If agriculture cannot offer them adequate revenues, they will join the ranks of the unemployed in Dakar or try at all costs to emigrate to Europe. \"Restoring, consolidating and developing the labour reservoir that constitutes African agriculture is one of the imperatives of the next decade\", comments Bruno Losch of RuralStruc.There is thus also a social dimension to the challenge of maintaining and improving family farm output. Abandoning this sector could lead to \"a massive and premature exodus of small farms that could overwhelm the capacities of many countries to cope\", warned Peter Hazell, of London's Imperial College, in Spore 125. A final consideration is that these farmers are the only ones able to safeguard their countries' natural resources, which are vital for everyone. They could turn this role to good advantage in the future, by using cultivation methods that preserve the environment and reduce CO 2 emissions (Spore 130).Modernising family farms and increasing productivity so as to make them competitive, while at the same time employing a substantial workforce for production, marketing and processing -that is the most pressing challenge. It can only be met by a radical change in policy. Decisionmakers need to wake up to the fact that family farming can be an engine of growth and that supporting this sector is a crucial first step. They need to offer assistance to the vast numbers of small-scale farmers so they can increase production and have better access to markets. It is vital that they revive agricultural research and extension, after a decade of neglect, that they encourage the development of irrigation, make it easier to access inputs and credit, improve the road network, electrify villages and help rural communities to emerge from their isolation through the use of ICTs.Making local markets more accessible by improving transport for products and access to price information can quickly lead to an increase of income for producers. Another approach worth exploring is the development of high value-added niche products, which can have valuable regional outlets or be used to make biofuels.To achieve these results, ACP countries must be allowed to equip themselves with appropriate agricultural policies that are adapted to their context, and protect their agriculture from competition. That is the verdict of small-scale farmers' organisations such as the Windward Islands Farmers' Association (WINFA) or the Network of Farmers' Organizations and Agricultural Producers in West Africa (ROPPA), which have put family farming at the top of their list of demands. These organisations deserve to be well supported and widely consulted, as they are the best placed to ensure that their members' needs and objectives are pushed to the fore once again, that they benefit from shared experiences and from the useful information they can make available. It is also important to give more space to women, whose dominant role in family farming is often overlooked, and to the young, who hold our future in their hands. n Spore 131 / October 2007 iN BRiEFIn South Africa, a pilot project is using enzymes to extract pectin from lemons and their zest. Pectin is a natural gelling agent used to reduce the quantity of sugar needed to make jam. The aim is to create an outlet for the tonnes of citrus fruit deemed unsuitable for export due to sub-standard quality -40% of the citrus harvest on the Eastern Cape. At a later stage, the public-private consortium behind the initiative is planning to develop powdered pectin for the export market.The Global Alliance for Livestock Veterinary Medicines (GALVmed) is a new initiative aimed at responding to the urgent need to address serious livestock diseases affecting the poor. The alliance, based in Edinburgh, UK, brings together researchers, research institutions, universities and pharmaceutical companies to produce new diagnostic tests, vaccines and pharmaceutical products, as well as improving ones already on the market. Two of the first GALVmed projects are being launched in Kenya. One hopes to develop a vaccine for Newcastle disease that is stable at high temperatures and available to small-scale backyard poultry farmers. The other aims to prevent East Coast Fever, which kills more than one million cattle a year, causing losses of over US$200 million (€148 million) in eastern and southern Africa.Pentlands Science Park Bush Loan, Penicuik Edinburgh EH26 0PZ Scotland, UK Fax: +44 (0)131 445 6222 info@galvmed.org www.galvmed.org A weed that was once deemed to be of no use to farmers is transforming the lives of producers in Kenya's Western region. Farmers are using the tree marigold, Tithonia diversifolia, known to locals as Maua Maruru, to boost soil fertility. The prolific weed, which is rich in nitrogen and phosphorous, serves as a highly effective alternative to expensive artificial fertilisers. The concept was introduced to farmers by researchers from the World Agroforestry Centre (ICRAF). Staff who studied the farms are showing producers how to cut the weed and leave it to rot on the soil. According to Julias Adiwo, an ICRAF senior research assistant, the quality of soils in most parts of the western region has long been affected by over-exploitation. Most farmers own very little land and cannot afford to buy fertilisers. Although the weed grows naturally in most areas of the country, very few farmers had noticed the important role it plays in enriching their soils, he added.Elizabeth Ondiga, who has begun using the technique on her 0.8 ha plot, says she can now harvest enough maize and beans to feed her family until the following season. \"Prices of fertilisers are shooting up by the day,\" she said. \"The weed has proved a far better way to rejuvenate my farm.\"The red goat of Maradi, in southern Niger, is making a new home for itself in the centre of Burkina Faso. When the Bazega rural development project first introduced this typically Sahelian animal, many livestock keepers were sceptical that it would be able to tolerate the humid climate and survive diseases such as pasteurellosis and trypanosomiasis. But the hardy red goat has settled in well, amply justifying the decision to introduce it into the area in a bid to improve the incomes of women and young people.The goat's fertility and ability to reproduce at an early age have surprised livestock keepers. Females as young as 10 to 12 months can produce offspring and regularly produce two or three kids. Even when a mother is suckling her young, a herder can still collect close to a litre of milk each day, compared with a scant quarter of a litre in the case of the local 'mossi' goat. At 3 months, the red goat is worth FCFA17,500 (€27), double the price of an 8-month-old local breed. The red goat's soft fine coat is much sought after in the luxury leather goods sector to make shoes, bags and clothes.However, wider-scale development of this breed is held back by the fact that few herders in this area milk their goats thoroughly, since the milk is only really consumed by children. If the udder is not entirely emptied of its milk, mastitis (inflamation of the mammary glands) can ensue. Project managers have started to train farmers in good milking practices. This will lead to more healthy goats, producing high quality milk that can be made into cheese, yoghurt and other products. The tree marigold boosts soil fertility in Kenya.The red goat of Maradi (Niger) has surprised livestock keepers in Burkina Faso.weeds to the rescue Two of the young men, Joeli Raviakara, 24, and Sanaila Tukutukuivalu, 35, have now won a scholarship for a year's further training in Indonesia. On their return, the pair will lead the Bitukao Enterprise production team. The furniture products will initially be aimed at local and tourist markets. When more young people have completed training, there are plans to investigate the export market, said Mr Korodrau.In Benin, nearly 400 pupils from eight schools in the municipalities of Dassa-Zoumé and Glazoué recently took part in the second final of an unusual inter-school competition. Organised by CPN-les Papillons, a local environmental NGO, and supported by CTA, the contest encouraged children to \"plead for the protection of the environment\" before an audience of around one thousand people.Helped by their teachers, the youngsters had earlier produced manuals on biodiversity, natural ecosystems, environmental degradation and ways of protecting the planet. A jury examined the manuals and listened to the young advocates, who were each given a maximum of 20 min to present their case. There was warm praise for all the entries and each school received hoes, watering cans, refuse bins or other useful prizes. The youngest contestants also received plants.On two Wednesdays each month, as part of the same project, students from three institutes present a radio broadcast called 'Nature and Us'. Translated into local languages Idaasha and Maxi, the programme reaches several hundred thousand list eners, say organisers. It deals with various topics including bush fires, desertification, recycling of plastic bags, compost making, deforestation and reforestation, ecotourism and the environment.Camaté-Shakaloké BP 16 , Sokponta, Benin cpnlespapillons@yahoo.fr www.oduland.com/cpn.htmIn the town of Dondo, in Mozambique's Sofala Province, members of a small wood-turners' cooperative have learned to turn a precious local resource into a high-fashion product. With support from the International Trade Centre (ITC) and ideas from designer and social entrepreneur Allan Schwarz, they craft high-quality bracelets that are making a splash at the high end of the fashion accessories market. Their design shows bold architectural forms that are selling well, featuring on catwalks and in glossy magazines. The move marks a turnaround for this poor rural community. Many were deskilled by years of civil war and previously trying with little success to earn a living making low-quality wood products for a tourism market that no longer existed.Founded by Schwarz, the Sofala Initiative, an alliance between a private company based at the Mezimbite Forest Centre, the Dondo woodcraft cooperative and the n'Hatanga community, has shown the forest community how to make the most of its wide variety of tropical trees, whose timber is durable and decorative, with rich colours ranging from deep burgundy to ebony. A replanting programme ensures that the wooden jewellery trade will be helping forest communities for generations to come. In Kenya, the Shompole Community Trust has won a top prize for conserving vast grasslands and savannah as part of a profitmaking ecotourism venture for the local Masai people. The trust was one of five community groups from tropical regions to win the UN-backed Equator Prize for their initiatives to alleviate poverty and conserve local biodiversity. Also named in the awards was the village of Andavadoaka in Madagascar, honoured for managing an octopus fishery so that it can provide sustainable longterm benefits. Each winning group will receive US$30,000 (€21,750).East Africa leads the continent in exports of certified organic products. The sector has received a further boost with the development of an East African Organic Standard (EAOS), launched in April 2007. EAOS is expected to enable economies of scale in training materials and certification, and create a unified negotiating position that should help organic farmers win access to export markets. But the potential of the new body to spur African organic exports could be seriously compromised by a move from the UK's largest organic licensing body. The Soil Association, which certifies more than 70% of retail organic produce in the UK, is considering withdrawing its endorsement of products imported by plane. It argues that air freight is responsible for 11% of carbon emissions and therefore at odds with organic principles. A decision is expected in November.Beekeeping is booming in Zambia. The Zambia Agribusiness Technical Assistance Centre (ZATAC) recently trained honey farmers in Mwinilunga District to produce certified organic honey for export to the international market. Meanwhile, in Solwezi, Kansanshi Mining Limited has brought in experts to train rural dwellers in beekeeping. One of the initiatives to emerge is Mutanda Honey, which buys comb honey from local farmers before processing and selling it to retail outlets. About 10,000 beekeepers in North-Western Province currently have 500,000 hives producing up to 1,000 t of honey and 100 t of beeswax annually, according to the Centre for International Forestry Research.The Coffee Research Institute (CORI) in Uganda has developed coffee trees that are resistant to the coffee wilt disease. \"The institute is trying out a wiltresistant Arabica coffee popularly known as Tuza. It is already doing very well in Bushenyi, Rukungiri and Ibanda Districts,\" said a CORI official. He explained that samples from the seeds have been tested and the results are promising. \"Plans are under way to take it to other districts, but it has to be done systematically to avoid mixing it with Robusta.\" A project to rehabilitate rice production has transformed Madagascar's Mandrare Basin from one of the country's poorest regions, where famine was rife, into a rice exporting area. The Mandrare project, funded by the International Fund for Agricultural Development (IFAD), restored irrigation systems, roads and other infrastructures and introduced more intensive farming methods. It also set up a network of microcredit institutions. The area now exports up to 25,000 t of rice per year. A second phase has helped farmers to produce an annual 200 t of cabbage, tomatoes, onions, garlic and carrots, as well as 4.5 t of corn and cassava seeds and more than 8 t of rice seeds. The initiative has encouraged local farmers to form producers' associations to market their crops.Over the past 5 years, the cassava brown streak virus (CBSV) has spread throughout sub-Saharan Africa. The virus destroys the root while the leaves appear to be healthy, so farmers do not realise that their crop has been ruined until harvest time. Scientists from the International Institute for Tropical Agriculture (IITA) in Tanzania suspect the virus is spread by people carrying infected cassava cuttings across Africa. They have now developed new varieties of cassava through cross-breeding, and trials have shown that these can successfully tolerate the virus. To speed up the spread of these varieties, the IITA is training farmers in a new method to increase the number of cuttings obtained from each plant.IITA-Tanzania ARI-Mikocheni (MARI), Plot 24B Sam Nujoma Road PO Box 6226 Dar-es-Salaam Tanzania Fax: +255 22 2775021 e.kanju@cgiar.orgIn ACP countries, older women are generally respected and listened to by their families and communities. However, until now, development officials have tended to focus on younger women to be partners in their projects. This approach is starting to change. A recent FAO seminar highlighted the key role played by grandmothers, dynamic women who in many countries of the South are often barely in their forties.An international NGO, 'The Grandmother Project', is working together with a number of community organisations in West Africa. In Senegal, the older women discuss female genital mutilation with the younger ones. In Mauritania, they are promoting good nutritional practices in the shanty towns. By combining traditional knowledge with an open approach to more modern ideas, these young grandmothers can contribute significantly to the success of projects for maternal and child health.Our elders, a resource for the future? In Spore 102, we observed that \"in most ACP countries the proportion of old people will grow for decades yet, despite HIV/AIDS and other ills.\" But much remains to be done, especially in agriculture, to ensure that they have access to adequate services for credit, training and obtaining equipment.The Grandmother Project (GMP) Via Aventina 30 00153 Rome Italy grandmotherproject@hotmail.com www.grandmotherproject.org/index. htmlThe most feared enemy of groundnut producers in tropical regions is aflatoxin, a substance produced by the Aspergillus flavus fungus. It poses a serious threat to consumer health as well as to the revenues of farmers, who cannot sell their produce on international markets if the groundnuts are infected. In order to obtain highquality groundnuts, farmers need to take precautions against contamination both in the field and at every stage of production -tracking aflatoxin \"from the farm to the fork\".The European New tools for groundnut aflatoxin control in Sahel Africa project has revealed that water stress occurring towards the end of the crop cycle encourages the development of aflatoxins. It therefore advises producers to use short-cycle varieties that produce small seeds, which have been shown to have greater resistance. After harvest, granaries and warehouses must also be treated to limit the spread of infection.Varieties with resistance to aflatoxin are currently being made available in West Africa. In Senegal, CIRAD and the main Senegalese producers' organisation ASPRODEB are seeking to build a quality groundnut sector by linking producer associations with private sector organisations.daniele.clavel@cirad.fr alain.mayeux@cirad.fr Unité de recherche « Agrobiodiversité des plantes de savane » CIRAD Avenue Agropolis -TA 70 / 01 34398 Montpellier Cedex 5 FranceSome 5,000 professional fishers from Mauritius (2,000 in Mauritius and 3,000 in Rodrigues) have all received 300 free shares worth 10 rupees (€0.23) each from the Fishermen Investment Trust (FIT). Created to help poor coastal fisher folk, the FIT has a capital of nearly €1.5 million made available by the government of Mauritius. Its activi-ties include fishing, the processing of fisheries products, the purchase of fishing boats and equipment, the provision of loans to fishers, job creation and a range of other benefits for fisher folk. Part of the capital is also used to fund training at the Mauritius fisheries school.The FIT is modelled on the Sugar Investment Trust which will also be investing in profitable fisheries activities and marine farming projects. The profits will be reinvested in aquaculture and in the harvesting and processing of sea cucumbers.Thanks to the FIT, fisher folk have priority in enlisting in these new projects. For a profession whose members are used to relying on no one but themselves and the sea for a living, that is quite a revolution. In arid regions of Africa, the production of gum arabic from the Acacia senegal is an important source of revenue, but output varies widely from one year to another depending on rainfall.In field trials on adult trees in Burkina Faso, Niger and Senegal, researchers have shown that productivity of the acacias increases by 25% if they are injected with rhizobia at the onset of the rainy season. These soil bacteria stimulate the growth of trees, strengthen their resistance to variations in rainfall and improve gum production. Scientists have identified the genes that affect flavour in coffee beans, paving the way for higher quality coffee and potentially widening the added value sector of the coffee market. The team, from CIRAD in France and Brazil's Agricultural Institute of Paraná, says it has pinpointed the genes responsible for sucrose accumulation in coffee beans. Sucrose is thought to play a vital role in the taste of coffee by releasing flavour and aroma during roasting. The next challenge is to find ways of improving sucrose content in beans to ensure better quality and higher earnings for producers.Howtopedia is a collaborative library for practical knowledge and simple technologies. Developed along the lines of Wikipedia, it enables visitors to exchange links and tips in a range of fields, including agriculture and energy. With just a few clicks, you can find out more about uses of shea butter and how to grow mushrooms on water hyacinths. Still in its infancy, the on-line library awaits your comments and suggestions.www.howtopedia.org/intro.php?L=enThe WAHID Interface provides access to all data held within the new World Animal Health Information System (WAHIS) launched by the World Organisation for Animal Health (OIE). Here you will find information on veterinary staff, laboratories and vaccines, as well as reports and maps presenting the animal health situation, livestock diseases and unusual epidemiological outbreaks in member countries. You can search by country, by group of countries or by disease, as well as find out about efforts to tackle disease and compare the animal health situation of two countries.Rabbit rearing has the potential to improve the diets and income of many African households as well as soil fertility. Rabbits mature quickly and reproduce rapidly. In good conditions, a single doe can produce 60 kittens in a year. They can be fed garden and kitchen waste, take up little space and are quiet, making them suitable for urban and suburban environments. They produce high-quality meat and useful manure.In the Caribbean, a number of initiatives have aimed to promote rabbit production among smallholders.The USAID Farmer to Farmer Program sent volunteers to Haiti to teach local producers how to raise rabbits. Production has reportedly increased in Cap Haitien and Portau-Prince due to demand from tourist restaurants. In April 2007, FAO supported a 4-H project in Rose Hill, Jamaica. In Barbados, the Small Grants Programme of the Global Environment Facility (GEF-SGP) launched a Sustainable Organic Farming and Rabbit Rearing Project in July with a gift of rabbits to a primary school. In Trinidad, efforts are under way to establish contacts between retailers and suppliers, and to set up a rabbit meat processing plant.One factor holding back further expansion is the so-called Easter Bunny syndrome. \"Some people think of rabbits more as pets, too cute to eat, like the Easter Bunny. Even some of the farmers who raise them can't bear to slaughter them,\" said Ansari Hosein, at the St Augustine campus of the University of the West Indies, which recently carried out a study on rabbit production.In Weight gain was poor when rabbits were fed on green leaf material alone. The animals thrived when fed with a low-cost supplement of cassava, banana, sweet potato, sugarcane or coconut. warm welcome for solar invention Kenyan John Maina, inventor of a solar dryer for fruits and vegetables, has been awarded the Energy Globe Award for the 'earth' category. More than 700 projects from 96 countries competed with proposals for clean and sustainable energy uses. Prizes, which were awarded in April, were given for each of five categories: earth, air, water, fire and youth.Since 2002, 30 of these dryers have been used to add value to harvests, increasing the revenues of nearly a thousand farmers linked to the Sustainable Community Development Services (SCODE), where John Maina is the coordinator. Some 30 craftsmen have learned how to build the dryers using wood and simple sheets of plastic. The invention is proving particularly useful in Kenya, where 30 to 40% of fruits and vegetables are lost for lack of affordable postharvest conservation techniques. Solar energy, which is free and widely available, saves using fuel wood, which is increasingly hard to find in the Rift Valley region.Meanwhile, Congolese inventor Mr Tsengue-Tsengue, head of Challenge Futura, a company that makes agrifood equipment, has developed a solar dryer which he says gives constant power and can be regulated. When there is no sun, the drier can use hot water to power it instead. A partnership has been signed with French company Atlas which is studying \"a simplified version so that even illiterate people can use it easily,\" explains the inventor.The device won the World Intellectual Property Organization (WIPPO) prize as well as an award from the Swiss solar energy association SWISSOLAR. Both prizes were presented at the 35th International Exhibition of Inventions, New Techniques and Products, held in Geneva in April 2007. Teak is a timber much in vogue, and as a result the demand for high quality plants is high. To meet the challenge, CIRAD has developed a system for industrially cloning teak plants, which overcomes two major stumbling blocks: the low rate of seed germination in plantations and the high level of heterogeneity of trees that grow from them. Several million plants grown in vitro have already been produced at very low cost.Contacts: olivier.monteuuis@cirad.fr jean-michel.sers@cirad.frSmall-scale fishers in Senegal have received their first ecolabel from the Swiss association Fair Fish. This certification sets fair conditions and a fixed minimum price for fishers and their families while at the same time seeking to conserve fish stocks. The label guarantees that fisheries enterprises respect traceability standards and use capture methods which minimise suffering to fish. At present, the fish is sold exclusively in Switzerland.Cité Sonees, Villa n° 1 Guédiawaye Dakar Senegal Fax: +221 871 47 55 marmindiaye2005@yahoo.fr www.fair-fish.ch/englishResearchers from the EU-funded Pumpsea project have launched a water purification system in a tourist area of Dar es Salaam, Tanzania, which uses mangroves to treat effluents from a hotel. The technique cuts faecal coliform bacteria in the water by almost 90%. Certain types of mangrove can filter nutrients, poisons and other contaminants found in wastewater, and could become valuable allies in the fight against pollution in subtropical coastal waters. The researchers have mapped most of the mangrove zones of Kenya, Mozambique and Tanzania, and identified areas where this inexpensive and completely natural technique could be applied.www.pumpsea.icat.fc.ul.pt/main.phpTen of the world's most important natural history museum and botanical libraries have joined forces to launch an ambitious project: digitalising all the publications and documents on biodiversity in their possession with a view to making them accessible to all on the Web. Nearly 1.5 million pages are already available for consultation on the website of the Biodiversity Heritage Library. It will take about 10 years to create the 300 million digital pages planned for the site, which will also offer videos, photographs and maps.www.biodiversitylibrary.org/About. aspxSmall-scale fishermen in Senegal are using mobile phones to market their products and to log their departures and estimated times of return, so that rescue services can be alerted if there is a problem. The Innovative Internet and Wireless E-services for Strengthening the Livelihoods of Senegalese Fishermen project is run by MANOBI, a private telecommunications company, in partnership with three local fishing unions, several other companies and international organisations. The scheme uses short messaging system (SMS) and Wireless Application Protocol (WAP) technologies. Fishers can also use their cell phones to access up-todate weather reports and market price information.The project has already produced good results. Fishers report increased incomes as a result of access to market data. One fishing union was able to send help to rescue an eight-man crew that had failed to return on time. The service also enables fishers to improve the quality of their products. By alerting potential buyers as soon as they land their catch, they can sell their fish while it is still very fresh. Typically, up to 30% of the catch of small-scale fishers is wasted while they wait to find a buyer.Whether they are shopping or eating out at a restaurant, environmentally aware South Africans can quickly find out if the fish offered to them comes from a threatened species. By keying in the name of the fish via SMS to a mobile phone number, they receive a colourcoded response from the FishMS service. Green means you can buy the fish; orange means the fish is legal, but stocks are threatened; red means selling the fish is illegal in South Africa. The service is offered by the Southern African Sustainable Seafood Initiative with the support of the South African government and the Green Trust.www.manobi.net www.panda.org.za/article.php?id=498Photo: © Syfia InternationalThe Ndiyo system enables several people to use the same computer at once.A simple new process for preserving coconut water could enable ACP small-scale entrepreneurs to tap into the growing market for this time-honoured tropical drink. The cold preservation technique was developed and evaluated in Jamaica by scientists from the University of the West Indies, the Coconut Industries Board and the Jamaican Scientific Research Council, with support from FAO. FAO has now published a free training guide, which clearly explains the process.Once exposed to air and warm temperatures, the quality of coconut water quickly deteriorates.\"The cold preservation process requires little investment and skills, and it offers small entrepreneurs a chance to enter the market of bottling coconut water of good quality,\" said Rosa Rolle of FAO's Rural Infrastructure and Agro-industries Division.The commercial production of canned coconut water involves sterilising the product using high temperature and short-time pasteurisation, a process that destroys some of the nutrients in coconut water and almost all its delicate flavour. The new process, which involves filtration, bottling and rigorous temperature control, protects the natural flavour of coconut water. It allows farmers to produce bottled coconut water that stays fresh from 10 days to 3 weeks. The cold preservation technology is not protected by patent and can be used by anybody.Good Practice for the Small-Scale Production of Bottled Coconut Water AGS Registry FAO Viale delle Terme di Caracalla 00153-Rome, Italy Fax: +39 (0)6 5705 4960 AGS-Registry@fao.org Detergent infused with citrus essence, citronella candles that repel mosquitoes, eucalyptus-based treatments to alleviate respiratory problems and -wait for it -socks impregnated with essential oils. Nature is a strong selling point right now and the agrifood, chemical, pharmaceutical and cosmetic industries are constantly on the lookout for natural substances that are just as effective as synthetic products but are also beneficial to health and the environment. The trend helps explain the massive upsurge in interest in essential oils (EOs), volatile fragrant substances produced by wild or cultivated plants. The most common method of extraction is steam distillation (see box) or, in the case of citrus fruits, cold pressing of the zest. EOs are not to be confused with the fatty oils extracted from oleaginous plants such as shea or jojoba.The price of these precious oils depends on the parts and quantities of plants used, as well as on the difficulty of harvesting them, a process mainly done by hand. One of the most costly EOs is Neroli oil, extracted from the blossoms of the bitter orange tree (Citrus aurantium), grown in many ACP countries. A tiny 2 ml bottle sells for around €13 on the Internet while the essence extracted from the skin of the fruits is between 10 and 15 times less expensive.According to the report Indian Oil Industry 2005, global output of EOs and citrus essences hovers between 100,000 and 110,000 t. Some 55% of production is accounted for by the South, led by Brazil, China, India and Indonesia, which are also major consumers. The international aroma and perfume market (worth US$6.3 billion in 2006, according to BCC Research) is expected to grow by an annual 4.5% in the coming years. The EU, Japan and USA are traditionally the biggest importers of EOs, but demand from Asian countries, especially South Korea and China, is growing rapidly. The boom in aromatherapy, a treatment involving the absorption, massage or inhalation of often organic EOs, is helping to create niche markets for high value-added products.ACP countries are well placed to profit from these trends. Their climate, the richness of their flora and plentiful supply of labour are clear advantages when it comes to growing and harvesting aromatic plants. Some countries have a virtual monopoly on plants with unique properties: Amyris balsamifera or West Indian sandalwood in Haiti, muhuhu (Brachylaena hutchinsii) in Kenya and Tanzania, buchu (Agathosma betulina) in South Africa, to mention those that are most widely marketed.The Caribbean has a long tradition of EO production. Despite strong competition, Grenada remains the world's second largest exporter ofSome 4,000 plant species contain aromatic essences, but only a few hundred of them have sufficient concentrations to allow extraction of essential oils. Steam distillation in a still is the most common extraction method. The parts of the plant -flowers, leaves, fruit peel or roots -are placed in a tank through which steam is passed. This causes the aromatic cells to rupture, releasing the molecules of essential oils. The blend of steam and oil then passes through a condenser -a coil that has been cooled down in a vat of cold water. When the liquid (hydrolat) emerges, the oil floats to the surface and can be harvested. The final quantity and quality of the product depend on careful monitoring of the temperature and pressure inside the still, and to an even greater extent on the quality of the plants used, which must be picked at the right moment. The quantity of EO harvested varies significantly from one plant to another, and this affects price. To obtain 1 kg of pure essence, you need to distil 1,000 kg of orange blossom and 600 kg of rose geranium (Pelargonium graveolens), but only 6 to 7 kg of cloves (Syzygium aromaticum). A more delicate process, extraction by solvent involves chemicals and is mainly used to capture the perfume of the most fragile flowers such as jasmine.Cold pressing is exclusively used to extract essences from the zest of citrus fruit (orange, lemon, bergamot or other). The equipment needed is inexpensive. You simply peel the fruit before pressing and centrifuging the skin to harvest the essence. The zest of 100 kg of citrus fruit produces between 500 and 850 g of essence.nutmeg oil (Myristica fragrans) after Indonesia. Haiti is the world's leading producer of amyris oil (Amyris balsamifera) and the second biggest producer of vetiver -see our field report -and produces citrus essences for liqueurs. Jamaica is known for its EO made from the leaves and berries of the pimento (Pimenta dioica and P. racemosa), while Dominica produces significant quantities of bay essence (Laurus nobilis). The search for new aromatic plants to develop in the region may help in the push for agricultural diversification and job creation.In the Pacific region, the Centre for the Development of Enterprise (CDE) is encouraging farmers to start producing EOs to help them penetrate the world market by offering quality products which, it says, are in growing demand. Two species of sandalwood have traditionally been an important source of revenue for the region: Santalum austrocaledonicum in Vanuatu and S. yasi in Fiji and Tonga. Three medicinal plants have been identified as holding out significant promise for the local EO industry: a variety of basil (Ocimum gratissimum), a type of mint (Plectranthus amboinicus) and a tree (Pandanus tectorius).Despite the rich diversity of its flora, sub-Saharan Africa still only plays a marginal role in the EO market. Notable exceptions are South Africa, which has a sizeable output of different oils (eucalyptus, geranium, camomile, lavender), Côte d'Ivoire (citrus essence) and Madagascar, a major producer of EOs extracted from ylangylang and cloves. Other countries, including Ghana, Malawi and Nigeria, have recently joined their ranks.In 2004, PAHE, the pan-African network for aromatic plants and essential oils, launched a campaign to encourage African countries to draw up SME development strategies for the production of essential and edible oils. The initiative was aimed at creating jobs and increasing earnings from traditional knowledge and non-timber forest products. South Africa has successfully created various support programmes for small-scale farmers producing EOs. In the Northern Cape, the country's Department of Science and Technology is encouraging the cultivation and distillation of geranium as a way of creating jobs and reducing poverty.EOs belong to a group of products which, according to the CDE, have the advantage of requiring little investment and simple, tested technologies that are easy to use and install in rural settings. They can make a significant contribution to boosting revenues for rural communities, take up little space and can easily be despatched by plane at reasonable cost. Production methods have to meet exacting quality standards to ensure that plants have adequate levels of active ingredients. Those that fail to do so command lower prices. Since EOs are complex substances, they require regular laboratory testing if they are to qualify for export. One important step is to determine their chemotype (see box) -their precise biochemical structure, which is strongly influenced by the ecological area in which a plant is harvested.As Spore observed back in 2000 (Spore 86), \"It is a rewarding, but exacting market to conquer, and one where quality counts.\" The EU imposes rigorous standards. The REACH system, which since 2006 has ensured control of 30,000 chemical substances, also applies to essential oils. It requires manufacturers to demonstrate that their products are not harmful in any way, a process that is beyond the means of most ACP SMEs, unless they have assistance.Adding to their difficulties is the fact that small-scale producers must also take account of specific market constraints, which may change dramatically from one year to the next due to competition. Producers need to be well informed of market trends, build up contacts with potential clients and make sure they hold onto those clients by supplying adequate quantities of EOs regularly. Even though producers in the South now have more direct access to the markets of industrialised countries -mainly due to the Internet -these markets remain distant, sporadic and exclusively based on raw materials. It is hard to find export markets for plant-based products, unless they are part of a long tradition such as Bay Rum in the Caribbean, a blend of EOs, including one extracted from Pimenta racemosa. This has long been used in Europe as an eau de Cologne or hair lotion.Most recent studies on EOs focus on the commercial opportunities that local and regional markets offer ACP countries. One example is a small-scale enterprise in Ghana which is seeking to tap the markets of neighbouring countries with an anti-mosquito lotion based on citronella EO (Cymbopogon nardus).EOs can be extremely useful in their country of origin where their antiviral, antibacterial and antifungal properties may help protect humans, livestock, crops and harvests. In Papua New Guinea, pharmacies sell waria waria oil distilled from Asteromyrtus symphyocarpa, whose antiseptic properties are similar to those of tea tree (Melaleuca alternifolia).A number of research initiatives are under way in ACP countries to study the properties of EOs extracted from local flora, based on Tropical essential oils are selling well in European pharmacies.An essential oil can contain dozens or even hundreds of components, which lend it its particular taste or fragrance. A gas chromatography laboratory test is needed to determine the percentage of each constituent. Two EOs extracted from plants belonging to the same botanical species can have the same components, but in very different proportions, depending on the place where the plant was harvested. The amount of sun and the type of soil on which the plant grew both play an important role. That explains differences in price and the fact that plants from some regions are more sought after than those from others.The active ingredients and their concentration vary widely between varieties. For example, there are several hundred varieties of eucalyptus, but only a dozen of them produce an EO, obtained by distillation of the leaves. The oil from E. globulus, cultivated in Malawi and South Africa amongst other places, is rich in eucalyptol.It is prized for its beneficial effect on the respiratory tract. Essence of E. citriodora or lemon eucalyptus, which grows mainly in Madagascar, has a calming effect due to its high content of citronellal, and is also used in the perfume trade.Photo: © Syfia International DOssiER traditional knowledge. In South Africa, branches of Lippia javanica have long been used to repel insects. The EO from this shrub has been found to be effective in warding off Aedes aegypti mosquitoes, which are vectors of yellow fever. When added to candles, the EO repels 98% of these mosquitoes, compared with 40% using citronella. In Burkina Faso, Cameroon, Congo and Kenya, tests are being conducted to establish the capacity of various plants to repel malaria-carrying mosquitoes.In Cameroon, several universities are working together to study the efficacy of EOs extracted from local plants to act as biopesticides. Some are looking at the potential of EOs to combat weevils and lesser grain borers, pests which cause serious damage to maize stocks. Others are comparing the ability of various citrus essences to fight Phaeoramularia angolensis, a fungal pathogen that causes spotting on citrus fruit and leaves, and hampers cultivation in many parts of Central Africa. At the heart of the network, the University of Ngaoundéré is focusing on the domestication of plants as sources of EOs and the dissemination of this information further afield.In Burkina Faso, a team from the agricultural research institute INERA has demonstrated the efficacy of EOs extracted from Cymbopogon citratus, C. giganteus, Lippia multiflora and Ocimum basilicum against rice seed fungi. In Madagascar, the antibiotic properties of EO of Cinnamosma fragrans, one of the country's many traditional medicinal plants, have caught the attention of researchers from the national rural development research institute FOFIFA and CIRAD, who see it as a potential substitute to the antibiotics used on shrimp farms.The success currently being enjoyed by EOs is fuelling a constant search for new plants with potentially beneficial ingredients.This can sometimes lead to over-harvesting of wild plant populations, especially in forests. Cultivation is the best option, as a way of avoiding serious damage to biodiversity while encouraging the creation of jobs and income for small-scale producers and distillers.Photographic credits: © C Jewell, © R Bosch, © F and K Starr, © www.geranium-bourbon.com, © Syfia International Pierre Léger has forged a cast iron reputation in his field. Frager/Agri Supply, the factory he owns in southern Haiti's Plaine des Cayes, supplies almost 70% of the world market in vetiver essential oil. \"I export more than 80 t per year\", says the 59-year-old agronomist, as he points to an array of samples on display in a corner of his sizeable office in Port-au-Prince. Becoming and remaining the world leader in the vetiver market is no mean feat, especially in a country as volatile as Haiti. The secret of Mr Léger's success can be summed up in a few words: always provide clients -mainly drawn from the European perfume industry -with top quality products, and always respect delivery deadlines.This businessman from the Caribbean's poorest country readily acknowledges that the 25,000 or so farmers who supply the vetiver roots are the real pillar of the industry. \"Frager/Agri Supply belongs to the community of the South,\" he asserts. \"The farmers play a direct role in its management; they monitor performance and sometimes tell me which decisions I should take.\" For Mr Léger, the country's glaring lack of infrastructure remains the main obstacle to the development of the essential oils industry in Haiti. \"The appalling state of the roads, combined with draconian electricity rationing and a lack of ports and airports have led a number of producers to abandon this sector,\" he laments.The head of the world's biggest vetiver factory is actively involved in community activities. Translating that commitment into training for the young, he offers study grants at Haitian and foreign universities. He recently donated 25 ha of land to the government for the construction of a university campus in the region.Since 2000, this towering entrepreneur -he is almost 2 m tall -has broadened his horizons: he regularly travels the world to share his expertise and help countries beset by poverty like his own native Haiti to set up essential oil production facilities. It all began when the International Trade Centre (ITC) sent him on a mission in Africa's Great Lakes region to evaluate the potential of these countries to produce EOs. \"At the time, essential oils were little known in Rwanda and Burundi,\" recalls Mr. Léger, who is firmly convinced that South-South cooperation is the most effective way of fighting poverty. Now both Burundi and Rwanda are on the point of becoming producers of quality essential oils, he says. \"Plantations of patchouli have been introduced there,\" he says. \"They have carried out trials and obtained the best quality patchouli oil in the world.\"Since then, his advice has been sought from all corners of the globe -from Asia, South America and Africa. But his responsibilities as company director in Haiti do not allow him to satisfy all the requests. He did, however, launch a partnership with Brazil last year.After 34 years in essential oil production, Mr. Léger believes the time has come to hand over the reins to the next generation. \"My father introduced me to this sector when I was very young and I have done the same with my son and daughter. The world perfumery industry has shown no hesitation in accepting them,\" says the agronomist. He himself took over the business from his father Frank in 1984, after studying in The Netherlands. The essence of the art, so to say.Jean Among the cargo of Captain William Bligh's Bounty, during its ill-fated voyage from Tahiti in 1787, was a consignment of 1,015 potted breadfruit plants. Though most of the breadfruit was lost in the mutiny that followed, subsequent trips by the infamous captain delivered plants to the Caribbean.Breadfruit was domesticated in the Pacific long before Captain Bligh ever sailed there, and has nourished islanders for more than 3,000 years. Aside from the Pacific and the Caribbean, the breadfruit tree also grows in some coastal regions of Africa, especially Mozambique and Guinea. But this species, Artocarpus altilis Fosb, is not to be confused with African breadfruit (Treculia africana) -a totally different plant -or jackfruit (Artocarpus heterophyllus) which looks similar. A multipurpose tree, breadfruit provides food, timber, medicine and natural insecticides, and is an integral part of home gardens for many ACP communities.Though grown in almost 90 countries, breadfruit is still an under-utilised crop, with often untapped potential for improving nutrition and increasing incomes. There is also considerable scope to improve the quality of the cultivated varieties and to develop processing to make more value-added products. At the CTA-supported First International Symposium on Breadfruit Research and Development, in April 2007, Aleki Sisifa, Director of the Land Resources Division at the Secretariat of the Pacific Community, observed that breadfruit is underutilised because it is given low priority by governments and research institutes.The symposium, held in Nadi, Fiji, explored progress in breadfruit research, conservation of genetic resources and food product development. A number of speakers stressed the importance of this crop as a healthy local food source, especially in the Pacific, where a growing tendency to consume imported processed foods is having an impact on health as well as the economy. Breadfruit is rich in energy, vitamins A, B (thiamine, riboflavin and niacin) and C, phosphorus and iron. It can be used to prepare a vast array of dishes, including appetisers, salads, soups, stews, casseroles, breads and desserts. Like banana and plantain, it may be eaten ripe as a fruit or under-ripe as a vegetable.Nutritionists and researchers interested in extending the reach of breadfruit say there is good potential for developing innovative food processing techniques, using traditional methods as a springboard. Fermentation offers one interesting possibility, adapting techniques used to make dishes such as masi ulu -fermented breadfruit biscuits from Samoa. On a recent visit to Pohnpei, food scientist Dr Richard Beyer demonstrated how breadfruit can be ground, extruded and dropped in hot oil to puff up like 'cheese balls', making a tasty local snack. Breadfruit chips -slices fried in oil -are made commercially in Trinidad and Barbados. Still in Barbados, flour made from the dried fruit is sometimes partly substituted for wheat flour in bread-making. Breadfruit flour is much richer than wheat flour in lysine and other essential amino acids. In Jamaica, the flour is boiled, sweetened, and eaten as porridge for breakfast.Surplus or damaged breadfruit can be used as livestock feed. In the Pacific, efforts are under way to encourage smallholders to use the fruit as the basis for a balanced swine feed. Tons of breadfruit unfit for human consumption go to waste each year while pig feed is imported.In some ACP countries, notably Fiji and Samoa, breadfruit is becoming an export commodity. Sangeeta Prasad, from Food Processors (Fiji) Ltd, says the company can barely keep pace with the growing demand from Australia, Canada and New Zealand. In Dominica and Trinidad, breadfruit is canned for shipment to London, New York and New Zealand. Other techniques developed to improve conservation methods for export include freezing and vacuum-sealing. Some Jamaican exporters partly roast the whole fruits to coagulate the latex, let them cool, and then ship them by sea to New York and Europe.According to Fiji Breadfruit Industry Development Project Coordinator Andrew McGregor, breadfruit has a large market potential with communities of Pacific islanders living in New Zealand, but better post-harvesting and presentation are needed. Breadfruit continues to respire even after harvesting, causing packing cartons to soften and collapse. Importers also require fruit to be graded by size and better packaged. Other efforts are focusing on improving the quality of the fruit itself. At Fiji's Ministry of Agriculture, the Research Division is developing ways of raising seedlings through different propagation techniques like marcotting (air layering), root cuttings and root suckers. Officers from the ministry are working closely with exporters to control pests. Meanwhile, on the Pacific island of Niutao, work is under way to make a soughtafter dwarf variety available to growers in other parts of the region. Negotiations with the islanders has resulted in an agreement to mass produce the tree -popular because of the ease with which it can be managed and harvestedfor distribution to smallholders on other islands.In Vanuatu, breadfruit is eaten roasted over the embers. This documentary looks at the potential offered by biofuels to sugar producers in Brazil, France and Mauritius. In this latter country, sugarcane is threatened by the end of the EU Sugar Protocol, which is expected to lead to a drop in prices of around 40%. Producing ethanol is one way of protecting the sugarcane sector and the jobs of the people who work in it, while at the same time reducing energy costs. The 52-minute film explains how and why large agricultural enterprises such as those in Brazil are entering this new market.The second part of the documentary looks at the likely impact on the planet of the biofuels expected to at least partially replace petroleum. It raises the issue of the potential damage caused by their production, if this is done at the expense of food crops, especially in developing countries. Hence the recommendation by many experts to wait for the second generation of biofuels based on forestry or agricultural waste such as straw. Researchers interviewed on the film warn that it is an illusion to think that the Earth can feed its 9 billion inhabitants and also supply them with energy. Cutting consumption of petroleum remains the number one priority. Published in 12 languages, this CD ROM offers a selection of articles presented at the conference in Nairobi, Kenya, in September 2005 on Participatory Geographic Information Systems (PGIS). These techniques enable marginalised communities to plot their territory on maps, ranging from simple sketches to 3D models. Traditional knowledge mapped in this way is a useful decisionmaking tool and facilitates communication between groups and with governments.The CD ROM presents a range of experiences from various locations. It shows how a community in Fiji has used PGIS to safeguard its cultural heritage, while in Ghana it has helped solve land rights problems. In Namibia, meanwhile, people have used the technology to promote community management of natural resources. Wherever it is used, PGIS can help local communities to establish their rights and make sure they are acknowledged by third parties. The CD ROM also contains numerous references as well as some short video clips demonstrating practical exercises. This unusual atlas makes a link between regional ecosystems and poverty in Kenya. Nature's Benefits in Kenya overlays georeferenced statistical information on population and household expenditures with spatial data on ecosystems and their services, such as water availability, wood supply and wildlife populations to yield a picture of how land, people, and prosperity are related in Kenya. With improved management in poultry keeping, the survival rates of chicks can be increased from three out of ten to eight. Any sweet potato variety can be dried to make chips, which can then be made into flour. Five new titles from CTA's Practical Guides series aimed at small-scale producers in eastern Africa, offer a host of such useful nuggets of information. All of the 8-page leaflets are clearly illustrated and simple to follow, with a good set of addresses for further information. Global Environmental Governance is an interesting read and a useful tool for anyone involved in this important but increasingly complex sector. The introductory essay explores the reasons why the international community is having problems in making headway on global environmental issues. The dictionary explains some of the terms that can act as a stumbling block during negotiations. One of the difficulties, it emerges, is that not everyone speaks the same language. Or rather, not everyone involved in preparing and reporting on some of the many conferences on the subject uses the same definition for terms that are widely employed.Jargon and acronyms abound in the field of Global Environmental Governance (itself an acronym, GEG). As the authors point out: With explanations for more than 5,000 terms, acronyms and organisations, this book should unlock the often impenetrable terminology and prove helpful to anyone interested in environmental or sustainability issues. Each year, almost 7 million t of fish bycatch -the part of a catch that inadvertently ends up in the nets -are taken by fishing vessels. Most of it is either discarded at sea or used for human or animal consumption. Shrimp trawling is generally regarded as one of the least selective fishing methods, and has a serious impact on populations of sea turtles, sharks, coral and other marine species.A number of ACP countries are working towards the development of bycatch reduction mechanisms. In Nigeria, a device called the fisheye has been effective in reducing catches of juvenile fish, while Mozambique recently made trawl efficiency devices mandatory on shrimp trawlers. Research is continuing in other countries, including Costa Rica, Trinidad and Tobago.This guide is the product of a worldwide FAO project to minimise wastage and damage to species taken indiscriminately. It offers technical information and construction details for many devices that have been proved to reduce bycatch in tropical shrimp-trawl fisheries. Full of clear illustrations, it explains how to select, install and maintain the devices.The Convention on Biological Diversity recognises \"the vital importance that women play in the conservation and sustainable use of biological diversity.\" This book explores how women can make a particular contribution. The authors examine the issue from a general standpoint -acknowledging that both women and men have roles, rights and responsibilities in ensuring the sustainable use of biodiversity resources. They also provide plenty of examples to support their argument. There are chapters on a number of ACP experiences, including biodiversity management in the wetlands of Kampala in Uganda, the role of women and biodiversity in fostering sustainable development in Cameroon, and strategies and constraints in the Gambia, Kenya, Lesotho, Mauritius, Tanzania and Zimbabwe. Using case studies from 18 countries taking part in the DFID Regoverning Markets programme, this book looks at some of the challenges facing farmers struggling to keep up with the wave of new demands made by food manufacturers and retailers. Among ACP countries featured in the study are Kenya, South Africa, Uganda and Zambia.The book explores the impact of the modernisation of food supply chains on the people whose livelihood depends on food production. It offers some solutions to living with this inexorable process, showing small-scale farmers that there is strength in numbers if they can group together. It also provides advice on best practices for small-scale producers seeking to be part of supermarket supply chains, and identifies some of the main obstacles which need to be removed. Floriculture is a growing sector for small-scale producers in Fiji and this useful manual will help readers find out more about its potential and how to get started. Packed with information and illustrations, the guide offers clear concrete advice on growing anthuriums, orchids, gingers and heliconias, from selecting a site right through to post-harvest handling.A Pictorial Handbook South Sea Orchids, 2007. 38 pp. South Sea Orchids Ltd (SSO) PO Box 570, Lautoka, Fiji Fax: +679 662 2283 sso@is.com.fjThe increasingly important field of biotechnology is not always easy to understand for those outside the sector. The FAO glossary of terms and acronyms commonly used in biotechnology for food and agriculture is now available on CD-ROM (Arabic, English, French and Spanish). A user-friendly search facility allows easy cross reference between the four languages. CrossRef, a non-profit organisation seeking to improve access to published scholarship through collaborative technologies, is extending its reach to include hundreds of journals from Africa. The organisation, whose linking service enables researchers to click on a reference in a journal and access the cited article, has reached agreement with the National Inquiry Services Centre (NISC) and African Journals OnLine (AJOL). NISC is registering its entire list of South Africanbased academic journals and bibliographic databases. AJOL, a fast-growing, independent journal aggregator, will contribute links to articles from more than 260 multi-disciplinary journals from 21 African countries.Publishers International Linking Association 40 Salem Street Lynnfield, MA 01940, USA Fax: +1 781 295 0077 info@crossref.org. www.crossref.orgThough gaining wider recognition as a major global problem, illegal logging remains poorly researched and understood. There are still few reliable figures on the extent of this phenomenon and many regard the issue as a simple problem of law enforcement. This book sets out to explore the many aspects of illegal logging, including its causes and implications for rural livelihoods. It also makes policy recommendations in the hope of reaching solutions to combat this widespread but complex scourge. Oum ar Sow is an advi ser on lives tock reari ng for a susta inab le rura l deve lopm ent prog ramm e run by an Aust rian NGO (EWA /PAD ER) in Loug a, nort hern Sene gal. The artic le on scho ol farm s (Spo re 127) brou ght back mem ories for him of his early year s at prim ary scho ol. \"At the time I was learn ing the first lette rs of the Fren ch alph abet and I used to watc h the olde r pupi ls, after their even ing lesso ns, rush out behi nd the class room s to wate r their carro ts, cabb ages , turn ips and aube rgine s... We liked goin g with them beca use they som etim es let us taste the vege table s they had grow n, whic h were delic ious . We used to go and get ferti liser (stric tly orga nic) for them , fetch ing man ure in whe elba rrow s and sack s from our fami lies' field s and barn s.\" \"In this way, the auth oritie s soug ht to use the educ ation syste m to mak e a link betw een scho ol and later life,\" cont inue s Oum ar Sow . \"The fact that I am now a vet and invo lved in rura l deve lopm en t is partl y due to the stron g impr essio n that this train ing mad e on me. I wou ld have liked to see this syste m beco me more wide spre ad, for deve lopm ent is first and forem ost abou t prod ucin g food so as to be self-suffi cient .\"Many of you already share information gleaned from Spore and pass your copies on to other readers -at work, in the village, in reading groups or within organisations. We are delighted to hear it, but we want to go one step further. With your help, we want to ensure that Spore is read by anyone who is likely to find it useful.For this reason, you will find two questionnaires inside this issue. We are asking you to renew your subscription and update your contact details. And we are also offering you the chance to help someone else take out a subscription.The orange form is for those of you who are already subscribed to Spore. Please complete it, taking great care to follow the instructions at the top of the page. This will help us to update your details and to better tailor our services to your needs. Do you know anyone who has never read Spore? If so, you can offer the green form to an organisation or individual in an ACP country working in the field of agricultural and rural development -whether it be in research, the agro-food sector, trade, knowledge sharing or agricultural training.We will give priority to organisations -including women's and youth groups -as well as to agricultural training centres and the libraries of teaching institutes.please note: as we have a limited budget we CANNOT accept individual subscription requests from students or farmers.Use the envelope supplied to return the form by post, and don't forget to add a stamp! If you have access to the Internet, there is no need to post the form. Simply go to http://spore-subs. cta.int, type in the access code on the back of the orange form and fill out the form, submitting it online only. In early June 2008, we will draw three requests at random from the on-line forms. The winners will be able to participate in a conference of their choice, on an agricultural or rural development topic, with expenses paid by CTA.So get to work! Thank you for your cooperation. The best way to understand the term 'New Agriculture' is to take a closer look at 'Old Agriculture'. Until very recently, agriculture was seen as a farm activity, based on primary commodities for both local and export markets. 'Old Agriculture' was dominated by the traditional export commodities sugar, bananas and rice, which enjoyed preferential treatment in the EU for access and price. After the Uruguay Round negotiations, it became clear that these preferences would eventually disappear. The result has been the partial or total demise of such industries, with substantial loss of jobs at farm and -in the case of rice and sugar -factory levels in the Caribbean region. It was apparent that Old Agriculture was dying. However, it still impacted significantly on the social structure of countries and the preservation of their fragile ecosystems, critical for maintaining the pristine environment necessary for tourism, a most important activity especially in the island countries.A different approach was needed if agriculture in the Caribbean was to be revitalised. This new concept soon became known as 'New Agriculture'. Given the dynamism of the international environment, it will continue to evolve. But for the time being, New Agriculture has certain key characteristics. It encompasses the entire agro-product chain, from the production of inputs, including machinery, to the sale of final products, whether fresh or processed. It also places emphasis on niche markets and value added products and is responsive to changing dietary practices for safe, nutritious and easily utilisable foods. New Agriculture includes nonfood items, such as nutraceuticals, biofuels, herbal products and handicrafts. It is driven by technology, especially biotechnology and informatics, and is a business and hence market-driven sector rather than one that is led by production. It requires a different set of extension, educational and research skills and programmes and is organically linked to other economic sectors, including tourism, education, health, transport, trade and finances.The success of New Agriculture will partly depend on government responsibility and responsiveness. Most importantly there must be total involvement of all stakeholders. For example, in St Lucia, where a hotel chain was looking for supplies of fresh fruit and vegetables, representatives from the hotel, the Ministry of Agriculture, local farmers, the Caribbean Agricultural Research and Development Institute (CARDI) and the Inter-American Institute for Cooperation on Agriculture (IICA) met and developed a programme of activities and targets. As a result, the hotel received a reliable and timely supply of products and the farmers earned increased income. There have been similar experiences in Jamaica and Nevis, and IICA is planning to introduce programmes in at least two other Caribbean countries.There are plenty of other examples of New Agriculture in action. One is the introduction of greenhouse technology to produce quality vegetables for national markets. Another is Haiti's Prohuerta programme, inspired by Argentina, producing commodities to improve diets. In Guyana, the Trilakes Community is producing organic pineapples for processing and export to the European market.Of course, there are hurdles. People have to be convinced of the need to dialogue with each other and agree on common objectives. All partners must have a long-term commitment and be determined to weather any storms that arise. At first, it may not be easy to convince small-scale farmers to try the new approach. But with the right encouragement and assurance that the inputs, technical support and markets are available, they will soon start participating, and once they are successful, we will see a multiplier effect. If farmers do not try New Agriculture, they are unlikely to be able to make a living from farming. At that point, they will have to move to other productive sectors, either in the rural or urban areas. If they shift to the latter, the result will be an added burden for national authorities seeking to provide appropriate social, civil and perhaps even penal facilities. n arlington.chesney@iica.int It was apparent that 'Old Agriculture' was dying.","tokenCount":"11595"} \ No newline at end of file diff --git a/data/part_6/0275102292.json b/data/part_6/0275102292.json new file mode 100644 index 0000000000000000000000000000000000000000..ddd2a1044d7bbaf6b518b468096e91d757cb8660 --- /dev/null +++ b/data/part_6/0275102292.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"ac32c8cf541d60a5c226ecfc8b356baa","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c2667699-3d68-4846-8b7c-85ad9802776a/retrieve","id":"-1467812248"},"keywords":[],"sieverID":"d8232293-da42-4e9f-9a6a-6efdcd17cdfe","pagecount":"65","content":"the specific crop programs revolve around breeding. ~le breeding itself is a numbers game with the following procedures:A. A world wide collection of germplasm is obtained so that there is sufficient genetic variability that sorne interesting characteristics can be comhined from different parental sources.The identification of the desired characters to over come specific constraints to yield increase is made.The \"relevant cionstraints\" on the production side are sorne combination of disease and insect pests, soil anel Hater conditions, and plant characteristics 2 .2/For example, rice breeding at IRRI was principally con--cerned with building sho~ter, sturdier varieties to respond to higher fertilizer levels without lodging with complete water control through irrigation. Breeding research was also directed at four diseases and threepests.Finally, non-photoperiod sensitivity was desired; however, this is a different type of characteristic sought principally by International Centers in order to give wider adaptability for the new material.See P.R. Jennings, \"The Amplification of Agricultural Production\", Scientific American 235(3) (Sept.1976), p.186; and p.R. Jennings, \"Plant Type as a Rice Breeding Objective\", erop Science 4 (,lan-Feb 1964), pp.13-l5.• .c.The \"re1evant constraints\" can be imposed by consumer 3 condi tions, such as taste preferences , as \\-1e11 as production factors.The germp1asm is screened for the characteristics identified in B. The best potential parents are identified 4 .D. These selections are then crossed and recrossed unti1 varieties emerge ,~ith the maximum of the desired characteristics.E. The varieties (or segregantsl are released to National Institutions for either dissemination, trials in different agro-climatic conditions, or further crossing for desired region specific charac-3/Consumers may not eat or may offer a lower price for a -bean of a specific color, size, or textura.In the cas~-of cassava consumers would be expected to prefer lower HCN content and a longer shelflife. 4/At this stage the selection process (before the initiation of the breeding program) may identify cultivars with a sufficient number of ¿haracteristics to be released into evaluation trials.Where there is high yielding ability but insufficíent resistances to disease and soil factors, these cultivars can he tíed to cultural practices and released as \"improved varieties\".In a horizontal resistance breeding strategy, the screeningis not so much for \"best\" parents as concerns resistance to pathogens but for wide genetic diversity without strong vertical resistance genes.It is thís stage that separates a horizontal resistance breeding strategy from this \"normal\" breeding strategy (see Appendix for discussion of horizontal resistanceJ. The International Canters are increasingly utilized for traioing younger scientists from variaus national organizations in developing countries. This proceso facilitates tha contacts for the successful operation of E above, 6!Part of the comparative advantage is physical.A larger breeding team can'specialize more and thereby produce a much larger number of crosses.Slmilarly, the interaction between agricultural disciplines should be useful for problem deflnition and solving.However, the most important advantage of International Centers may result from the \"mínimum critical investment\".Breeding requires highly trained personnel, spacialization in a specific crop, is expensive, and is a long term investment.National governments in developing countries generally havefew trained agricul tural scientists and have to be eoneerned with many crops.Moreover, research ls generally given a low priority in publie expenditures and decision makers in developing countries tend to prefer investments with a short payoff periodoThe advantages of the Intarnatíonal Centars are team size, specialization, large scale funding and continuity.This combination is considered by international donora to have a higher probability of reaching the \"mínimum critical investment\" for breakthroughs in new varieties than similar funding of most national systems.. '• 5 ternational Centers in relation to National Institutions is in their ability to diagnose desired new variety characteristics for a series of specific regions in a large number of developing coun-7 triesThe crucial decisions are in the definitions of the \"relevant constraints\". The rest is a more mechanical process of collecting germplasm (A), screening and crossing IC and D), and disseminating (E).International Centers are continually in a process of gathering, refining and digesting this information about the \"relevant constraints\" for the critical breeding decisions.This information gathering can be divided into three stages:7/0ne dilemma of International Centers is the development of a -methodology for obtaining more systematic definition of the \"relevant constraints\" from National 1nstitutions before the new material is released. The present tactic is to begin re leasing something aS soon as possible such as the better se~ ~ections (under C) or nintermediate technology\".The final product of 1nternational Centers is new varieties. However, in the process of mounting a breeding program, the other agricultural sciences generally identify a series of practices, which increase yields under experiment station conditions.Examples of these~intermediate technologies~are clean seed production, fertilizer response and spacing alternatives, andherbicide recommendations for different soil types.By identifying something profitable at the farm level the 1nternational Centers can build up better institutional ties with National Institutions and encourage mOre of their input into research design in the early stages of the process.Unfortunately, experiment station technology is not always relevant to farm level conditions.The technology may not be profitable, it may not fit into the existing farming systems, or it may increase risks much more than farmers are willing to accept.Hence, farm level testing is critical to evaluate whether the \"intermediate technology\" is relevant. are continually in a process af gathering, refining and diges! ing this informatian about the \"relevant constraints\" for the critical breeding decisians.'Ehis infonnation gathering can be divided into threc, stages: The objective oi this paper is to provide case histories of tha role of these three types of information on the r~search design of beal1s 2\"nd ca5sava In CIl\\.T. Obviou51y, this process is continu~ 1ly e'101'1ing so that the paper I5 only our snapshot oi the\" Dr~,sent si tuation.The available macro data is sketehy. Production data is unreliable when home consumption is important or there are many retail outlets. Area i5 rarely exactly measured and these erops are often produced in multiple cropping systems.Information on agricultural systems i5 rarely produced. Nevertheless, the macro data is \"useful to indica te trends and to make some inferences about stiategy. For all tlle Latin American countries including Mexico and Colombia mean yields show extreme fluctuation (see Figures 2-5).This extreme annual variability is the principal characteristic of Latin American bean production.Any research strategy for bean production in Latin America has to concentrate on Brazil and Mexico.The extreme yield variala tion indicates the riskiness of bean production.The next step in 9/J. H. Sanders y Camilo Alvarez P., \"Tendencias de la Produc ción de Fríjol en América Latina -11\", mimeo, CIAT, Cali, Colombia, Julio 1977, pp. 4, 18, 26. 10/Bean area but not yields would be sensitive to changes in ~ national policy or economic conditions. Substantial between year fluctuation in bean yields vould not be expected in res ponse to changes in relative or absoluta profitability. cassava, so that prices tend to be low and consumption concentrated in lów-income groups or on-farm subsistence. 80th factors then lead to the generalization that cassava ls primarily a subsistence crop grown under small farm conditions. Such generalizations, however, should be supported if used as the basis for a breeding strategy.The following section shall deal with whether the macro data support these assumptions about the location of cassava and the predominance of small farmers. age-icultural ilTeas 2nd \"S vell exhibits striking yield stability.Sli~ltly !ligher yields are noted outside the North anc1 Northeast.In the Northeast, small farms (less than 10 hectares in size) account for over 70 percent of the cassava area harvested.In the primary agricultural areas of Brazil, the South and Southeast, modal farm size for cassava producers is somewhat larger, being between 11 20 and 50 hectares . Cassava in this latter area is produced under a wide range of technologíes from small scale hand cultivation to Tab1e 2. Brazi1 - industrial utilization.The Center West and to a les ser extent the North rep-resent the frontier expansion areas in Brazilian agriculture.The principal activity of these areas is livestock production.Here cassava is primarily produce for subsistence, given the lack of infrastructure and the long distances to markets.Parms on which cassava is cultivated tend to be larger due to the large pasture component, but cassava plots themselves tend to be small.,Brazil except the Southea~t during the last decade (Table 2)In the llortheast cassova production has increased slightly faster than lhe population growth rateo In this small farm area cassa\\¡a Serves as a major subsistence food crop and demand (and therefore supply) expansion is principally a function of population growth. This subsistence role of cassava is highlighted in a 1960 household budget survey of Brazil (see Table 3). Consumption in the rural areas is three times that in the urban areas and per capita consumption in the rural areas of the Northeast is almost three times the level of the rural areas in the South andSoutheast. Since the cassavaproduction system in the Northeast is based• u.pon small farm production, a large part of the cassava produced is consumed, if not on the farm, w.ithin the local rural market area.In areas where cassava production takes place on large farms, per capita consumption falls, implying more is marketedTable 3.Ilrazil -Cassava Per Capita Consumption by Area in 1960.Total on a Fresh Basis -------kg.jcapita per year ----------- The East contains two states of the Northeast (Bahia and Sergipe) and the rest of the states of the Southeast, except Sao Paulo.The North and Centar Wast were not included. Bo\"h systems of production \\vould indicate that demand factors will be í'\",por t.ant in determining the adoption of ne\", varíeties particu-1ar1y as tl,e desired plant characteristics tor human consumption, animal feed, starch,and alcohol production are different 13Before re turning to demapd in thc j.nferences section the nex t S0Ctioll \\;tIl examine the a\"l:a on farm level constraints to proclnct:ion increase.Farm level constraints to the introduction of ne'\" technology have been measured in two ways, the Benchmark and the Gap approaches.In the Benchmark approach variation in yields on farmers' fie1ds with present cropping systems and varieties is 14 ana1yzedThe Gap approach attempts to explain the differences l3iror human consumption reduction of HCN and improved storeability are the critical characteristics.Whereas for the other uses, higher starch content is the critical desired characteristic.l~/This technique estimates the effect on yields of various con s traints with present varieties and systems. These estimates are expected to understate the yieId losses of new, higher yieIding varieties unIess the new variety were more resistant or tolerant to the spepific constraint.•1 ~/Elsewbere the single cropping and multiple cropping systems were compared. Qne explanation for a multiple cropping system or at least for diversification\" is as a risk avoidance mechanism.. Large farmers due to greater wealth can take more risks.They utilize rn~re inputs and specialize.See Camilo Alvarez P., \"Análisis Econ6mico de Algunos Sistemas de Producción de Fríjol en Cólombia, 1974-1975\", CIAT, Cali, Colombia, Marzci 1977, mimeo, 17 p5ginas., for a desc~iption of the two syst~ms.The risk avdidance hypothesis was tested with two years of experimental data from CIAr from 20 experiments. It was found that at the Colombian prices single cropped beans were more profitable and riskier than the beans-corn erap combinat.ion.See C.A.---rrancis and-J.H. Sanders, ' \"Economic An~lysis of•~ean and Maize Systems: Monoculture Versus Associated Cropping,\" CIAT, Cali, COlombia, Ju1y 1977, mimeo, 29 pages,.Risk avoidance, improved absorption of family labor and nutrition are a11 p~tential explanations for diversification rather than multiple cropping.The principal explanation f.or• rnultiple cropping is sorne type of cornplemen-~arity.CIAT experimental results comparing associated cropf1ing of 'c•orn and ~ea.ns with the mono-culture systems show less ihsect proble~s in both crops, les s ladging of corn and more nitro gen fixation by beans in associat~d cropping.Also planting beans beneath the corn should protect the soil better than corn alone especially on sloping and high or intense rainfall areas.See Further research in these areas of complementarity esp€cial1y in entomology.and micro-biology is continuing at elAT.There is also work on the physiology of the interaction of various systerns.The bencfits of these complementary effects hdve to be compa~ed with the costs of the effects from 1ight, water and nutrient competition. . ' < This is the total area available to the farmer . Nith more than one system of beans, perecntages refer to the number of farmers in Bean equivalents are caleulated by utilizing priees af ather commodities relative to beans as follows: Yield(beans) + Price (eorn_)_Yield(maize) \" Yield(bean equív.) Price (bean) The bean cpop in the Valle reglon ean be g~own in 3.5 months and followed by another c'rop.Refers to the second intarcropping combination cf beans/maize/potatoes, beans/maíze! \"at\"racacha lt and others.CIAr, Annual Report 1976, Cali, Colombia, p.A-74.-The constraints limiting bean yields in the two types of systems were evaluated utilizing production function analysis (Figures 7 and 8). Assuming that the samples were representative of the regions the economic losses associated with the disease and insect pests in one production season in these regions were substantial (Table S). There are series of disease and insect , 18pests attacking beans wi th dif ferences between the 'two reglonsThere appears to be a very high payoff to obtaining resistance tb any one or a f ' 19 combination o the above constralnts .Approximately, 95 percent oE the beans produeed were soldame consump~lon ,eSB t an one pereent o pro uctlon i:110 lügh bean pricgs Ln Colombj,a and the risk from storage :insecto; t]¡e félrmer did not obtain tile nutritional benefits of inCrOQ3ed bean consumption.ln Colombia, beans were elearly a eash erop ,in this semester.l~These differences in disease ineidenee between regions have been prineipally attributed to the altitude differenees. We are indebted to various other members of the Bean Team for this observation.Outside of the larger farms in the Valle bean producers did not recognize the existence of disease and insect problems. Low yields were generally attributed to soil fertility or 'weather in spite of the supermarket of pests encountered in farmers' fields.Unpublished data from these field interviews \" in Huila and Narino.19/5ince these results are time and location specific, this type of snapshot of yieldconstraints would be much more useful if it could be obtained for a series of regions over a longer time periodo However, these field surveys are expensive. Each of the 177 farms was interviewed three or four times by agronomists trained to identify the insect, disease, and weed problems of beans. .. .not appear to be a constraint to expanding cassava cultivation.1 ! \"The largest cost component in the cassava production system 20 is labor . Weeding, is the principal labor activity and the most costly operation. Fifty-six percent of the labor utilized in cassava production is devoted to weeding as compared with 21 percent for land preparation and planting and 8 percent for harvesting. Given the adequate available land in most regions, seasonal labor for weeding may be a constraint to cassava output expansiono lIowever, only three percent of the farmers utilized as expected herbicide an~hese were larger farms.The study supported the generalization that cassava is usually grown on poor soils (see Table A-12 in the Appendix) .Most of the soi1s were low in phosphorus (except in zones 11 and V), 10w in potassium (except in zone 11), and moderate to high1y acidic (except in zones 11 and V). As soils in zone Vare typi-20/This level of labor utilization is similar to that of the Northeast of Br~zil. • .)\"lt An important finding of the study sample ,vas that 99 pe.!::. cent of the cassava was marketed (see Table A-16 in the Appendix) .In the small farm areas of zone 111 where subsistence consumption would be expected to be high, farmers retained only 6 percent of their production for use on the farm.Horeover, in all other zones the percentage retained \\4\"-\" negligible. Cassava has a long storage life in theground b~t minimal post-harvest durability, a maximurn of 48 hours for most varieties.Hence, the farmer apparently waits until he ha\" a market ana su F-f icient labor to harvestthe crop. Th,?importancR of marketing land prices) and labor availability in ca SSél Vil. productton systern.s J. s very clear.In summary, weea control appears tobe a critical con-Cassava is predominantly produced by small farmers on infertile soils with minimal purchased inputs. Under these circumstances a new cassava technology based on high input utilization would have little chane e of adoption and in turn minimal impact on national yield levels 24 , Hore information is necessary on the 23/The impact of weed competition on yields of cassava ha ve --be en experimentally tested at CIAT. (see CIAT, Annual Report 1973, Cali, Colombia, 1974, pp.104-l05).24/Cassava production on the large commercial farms in --zone II is an exception to this.The factors contributing to cassava production in this area and the other regions in Colombia will be discussed in the following section.economic lossesthroughout the target are a resulting from the three diseases mentioned 25 .In the next section the sources of yield variation will be more systematically analyzed.25/Experimental research on the impact of Thrips attaek --on susceptible eassava varieties in the Cauea Valley showed that Cassava yields declined by'only 8-15 percent under attaek.Based on this researeh it was eoneluded that \"breeding for yield potential had priority over breeding for thrips resistance\". See A.v. Schoonhoven and J.E. Peña, \"Estimatíon of Yield Losses in Cassava Following Attack from Thrips\", Journal of Eeon. Entomology, 69(~), p,S16 .. ..The sample survey confirmed the low productivity of cassava production in Colombia. Average yield levels were 6.2 tons per hectare (fresh weightlas compared Hith consistent yielc1s of.over 20 tons per hectare of selected CIAT varieties in the Colombian .1 . 1 26 rC~lona trla s . The variation around this mean \\-las large, a standard deviation of 6.5 ton s , \\-lhich reflectec1 principally the yield rJifferences bet\\-leen producing regions (see Table 8). An un-c1c,,:standing of tlle f-a.ctors that contribute to the overall Using multiple regression analysis, the limiting factors on yields of cassava were delineated. The \"relevant. constraints\"were soil factors and diseases (see Table9). Purchased inputs, plant population, and weed control were not significant, which would.indicate that yield limiting factors were regional in their impacto Inter-regional differences in soil and clima.te appeared . .• . ' .' Where the principIe cassava diseases were found, there was a large reduetion in yield, contradicting the usual finding that cassava ia highly resistant to diseases and pests, Control of either Superelongation or Phoma Leaf Spot would resul t in an increase of almost 3, S, tons per hectare on affeeted farros as compared to an average yield of 6.2 tons per hectare.Control of Cassava Bacterial Blight would have added a further 0,75 tons to yields on affected farms.However, in this production sea son the area affeeted by these diseases was relatively minoro non,\" of these diseases affected more than fiv\" percent of the caSS2V'1 area, Thus, based on this samFle control of these discases would inerease average yields in the eountry by no more U\\C'n S percent nr e, 3 tons per heetare (see Figure 9). However, for individual farmers in areas where these diseases are prevalent, disease control would have a signifieant impaet on yield.Intercropping also resulted in a yield reduction of 1.8 tons per hectare.As only 31 percent of the eassava area was 28/Experimental trials at CIAT ha ve shown that cultural practices such as plant population, weed control, and use of fertilizer do have a significant impact on yield. These findings would not contradict the conclusions h~re as variation in cultural practices would be expected to have,an impact on yield level, of the high-yielding varieties used at CIAT, Within the farm sample the low-yielding varieties and the impact of other factors override the impact of cultural practices.This would imply that cultural practices may become a much more important factor with the release of new high-yielding varieties. , .. However, profitability and labor eonstraint eonsiderations enter into whether sueh a reeomrnendation should be made.If eassava and maize (the major form of erop association) intercrop yields are expressed in terms of cassava eguivalents, differences in yields between monoculture and intercropping were insignifieant 29As Figure 8 illustrates differenees in soil faetors aeeounted .ror much of the difference between elltrent average y1elds and potential yields based upon eurrent varieties and syst0ms oi prodLlction. J!jgh soil aeidity, low levels of phos-P lorus, éln I eavy soi. tGxtnre a contribute to ower yle .. ( s !Yom 60 to 70 p~rcent of tho eassava in tl1e sample was grOl-iD llnc1er tllese conditiolls. 1'he pl: illC ipal a r:é;a where these poor soil conditions were not found was in zone 11. These findings suggest that most cassava is grown on either highly acidic or low fertility status soils or both. Cassava does perform relatively well compared to most other crops under such.adverse conditions, giving yields in the three to six ton range. This partially supports the contention that cassava is grown primarily 29/Camilo Alvarez P., \"Anál'isis Comparativo de Tres Sistemas de Producción• de Yuca,;'. R.O, Díaz y P. Pinstrup-Andersen (editores), Descripción Agro-Económica del Proceso de pioduce ión de Yuca en Colombia. CIAT, Cali, Colombia, Junio, 1977, pp.L-1-2 1 ¡.2Q/In the regression all three factors entered as dummy variables. Phosphorus was stratified above and below 15 ppm, soil acidity aboye and below a soil pH of 5.0, and soil texture between the predominance of light or heavy texture soils. percei ved uneconorni.c response or higher profi tanili ty from lalld rotation than from increasec1 input use.Recognizing that there are constraints on input supply markets, the capacity of output markets, anc1 extension and technical services, especially to smallholc1er producers, as well as capital and risk constraints at farm level, the probability of success of a high input technology for cassava was consic1ered low. Rather, wic1espreadincreases in farm-level productivity were consic1ered to be likely to be obtained by developing new high-yielrling genotypes, resistant to high soil acidity anc1 ac1aptedto minimum levels of purchased inputs 31 Horeover, as the above micro data suggest the development of such varieties do es not preclude a yield response to higher fertility levels or pesticide application, should the price incentives for fertilizer and pesticide use become sufficient1y great.The C~~t~eat Inne~enee Stage 'The availab1e rlaero and Hiero data indieate some cwnc>ra1 direetions i.Tl both programs but still leave gaps in the defini.tion of the \"yelevant constraints\". These gaps have to he bridged by inferences élbout Latin American pro(lt¡c-t.i.on of these t\\IJO cOll}lT1odities. These inferences come frolTj members of the te.am and cthers 'dith experience in Latin America. Obviously, it is important to verify or reject these inferences with the col1ection of better field data in the future.Finally, the definition of a \"relevant constraint\" is not sufficient for it to be included in research designo The other necessary compo~ent is the subjective decision of the breeder that the desired characteristics to overcome the \"relevant constraint\" can be successfully incorporated into The major insect ~/This extremely useful division was made by Douglas Laing, Physiologist of the CIAT Bean Programo For further detail see CIAT, Annual Report 1976, Ca1i, Colombia, 1977, pp.A-57, 68. 3~/Under experimental conditions climbing beans have demonitrat--ed the potential to fix nitro gen to equivalent levels of ~1 kg/ha. of nitrogen.Since beans are predominantly found on small farms where few input s are purchased, this 1s a potentially very important resulto See CIAT, Annual Report 1975, Cali,Colombia, 1977 p.A-22. ~JThese inferences in r. were based upon the 1dent1fication by the scientists working in bean production in Latin America of the diseases and insects in their respective countries (see Tables 10 and 11l.After this survey was taken Golden Mosaic becam~ an important problem in the principal bean production regions of Brazil and Central Amcrica. Table 11.Majar insect peses of Seans (Phaseolus vulgarisl and their importan ce j)y' COllntry in Latin America. The crucial operating decisions of the Bean Program were then that beans of many colors and plant types would be sought.Secondly, high input packages would not be relevant unless beans were able to move into the better soil areas of Latin l;merica vThere large farmers predominated. Since beans had not been able to capture these areas previously and high value export crops with a long tradition of research and developed infrastructure for marketing would have to be displaced, the potc~n f:.{al for beans to enter these areas on anything more than n. short t8ym b;)sis Has considered to be a \"long shot 1l36 . Assuming away the resouree eonstraints of this group makes re-.search design easier and experimental yields higher. However, it increases the probability that the technology will not be relevant to farm level conditions in Latín America.Present constraints on bean production are a series of disease and insect pests, laek of water control (irrigation), and use of few eash inputs. Henee, the stress of the Bean Program has been on breeding for resistance to the majar diseases, tolerance to Empoasca, and breed1ng for high nitrogen fixing ability rather than assumine that farmers will be willing to purchase large amounts of chemicals for disease and insect control and fertilizers...n' me\"Ol?2 : -571\" 7 7 paZ? 7=\"-'170:7 7't mzerrM\"W• -e !trw'ttWiWtKr&H >0-' • . . . •fXj '~Wi'*t!'%tM >j\"\"tif'W7ifffl.;,i¡fMt-.Given the riskiness of bean production and the prevalence and seriousness of a series of diseases anJ one insect, the principal objective of the research strategy would be to achieve resistance to a multiplicity of diseases in beans of various color s and ideotypes.This focus on resistance was based on selection and breeding to the major diseases.Both vertical and horizontal resis-37 tances were sought depending upon the particular pest The use of sorne vertical resistances in beans can be justified for the following reasans:(1) the di.scontinuous nature of bean production and the mul Ur1.icit'f oE ü;JeotypelS should provide sufficient epidemic cOntrol should a particular vertical resistance break down, and(2) there are a number of methodological problems with beans in breeding for horizontal resistance.The first point stresses the fact that the spread of bean disases is limited because beans in Latin America, unlike grains, are produced in widely separated pockets. Also, it is unlikely that any one bean variety will become widely distributed due to preferences for different colors and ideotypes. Thus, any breakdown of vertical resistance will tend to be localized Even if a vertical resistance breaks down, the benefits of a few years of successful protection are often much greater than the costs of the resistance breeding.In 1) Area planted to cassava in Zone 11 makes up only B percent of the total Cassava area in Colombia.2) After Veoez~ela, Colombia has the highest price for cassava of aoy country in South America (see Table A-13 in the Appendix). Farmers.in Zone 11 receive the highest prices for cassava of any area in Colombia.Furthermore, caSSava prices in the seventies in Colompia have been at their highest level since 1955.See R.O. Díaz and Per Pinstrup-Andersen (eds.) (1977), op.cit., p.A-14.3) Yield levels in Zone 11 are already relatively high.In the. study samp'le large farm yields in this zone averaged 14.2 ton s per hectare and ranged as high as 52.0 tons per hectare.Thus, farmers in this arca are already exploiting a high yield advantage under high prices 1evels. This conclusion is supported by the information available on nat income par hectare for various crops (see Tahle A-14 in the Appendix). Currently, in Zone 11 cassava would displace most other crops except rresh vegetables, plaintains, and•coffee.However, prices are at the extremely high leve~ of U3$100 per ton.If prices dropped merely to the current price level in Zone V, net income would be almost zero.In Zone V as a comparison, cassava has on1y some advantage over maize and sorghum ano even then on1y at the high price leve15 (US$S5 per ton).In that zone ~assava is usually grown on poorer 50ils.As new high-yielding varie~ies ar~ introduced, cassava príces would be expected to fallo In thi5 circumstances 'demand considerations (particularly the price elasticity of demand) becomes the relevant determinant of whether _.~assava with higher yielding ability would break into commercial large farm a~eas.As the price elasticity of demand is low, this would be considered unlikely pnd, a cassava technology oriented initially toward current prbducers, would have the greater impact on raising production levels. vegetatively propagated {a clone) and is not season bound, there is both spatial and sequential continuity of identical hest tissue. If resistance were vertical and broke down there would have been no evolutionary survival value, thus the necessity fer horizontal resistance in its evolution.This factor provides support for the usual generalization that cassava is highly resistant to diseases and pests, though as CIAT trials have shown this may not be so for any one particular cultivar against all pathogens.. '\" . \",-,~ {","tokenCount":"4737"} \ No newline at end of file diff --git a/data/part_6/0293054610.json b/data/part_6/0293054610.json new file mode 100644 index 0000000000000000000000000000000000000000..597bd8687bee9e5f80b354e11b83e696aeb861b1 --- /dev/null +++ b/data/part_6/0293054610.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"030b5fd0d74197abf1e5a879936591ac","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/512a3530-af7c-4943-9238-1a6238f0e801/content","id":"-511936337"},"keywords":["molecular markers","pathotypes","resistance","stem rust","wheat"],"sieverID":"99558e15-0b9b-45ca-8f6b-537e8cc4e2cd","pagecount":"9","content":"In Central Asia, stem rust (Puccinia graminis f.sp. tritici) causes considerable damage, especially during growing seasons with high rainfall. Ug99 is a race of stem rust that is virulent to the majority of wheat varieties. To develop disease-free germplasm, wheat material was screened using the predominant stem rust races of Kazakhstan and tested in two nurseries; CIMMYT-Turkey and the Plant Breeding Station at Njoro, Kenya. A total of 11 pathotypes of P. graminis f.sp. tritici were identified in Kazakhstan from the stem rust samples collected in 2008-2009. In particular, pathotypes TDT/H, TPS/H, TTH/K, TKH/R, TKT/C and TFK/R were highly virulent. Of the 170 advanced lines of wheat, 21 CIMMYT lines resistant to 5 aggressive Kazakhstani pathotypes of P. graminis were identified. A high level of resistance was observed in 11 wheat cultivarsThe region of Central Asia is one of the most important wheat growing areas in the world. Wheat is grown on 15 mil ha, including 5 mil ha of winter or facultative wheat, and 10 mil ha of spring wheat. Kazakhstan is one of the largest wheat producers in Central Asia. Wheat rusts are an important problem in Kazakhstan and are one of the major factors reducing the productivity of wheat. Stem rust (Puccinia graminis f.sp. tritici) causes considerable damage, especially during growing seasons with higher rainfall. The stem rust fungus attacks the aboveground parts of the plant. In turn, infected plants produce fewer tillers and set fewer seed. In cases of extremely bad infection the plant may die. Currently, more than 60 numbered or temporarily designated stem rust (Sr) genes for resistance to stem rust have been listed in the Komugi Wheat Genetics Resource Database (http:// www.shigen. nig.ac.jp, accessed 26 June 2011). Utilization of many of these genes in breeding programmes has S147 resulted in the effective control of stem rust in most countries (Visser et al. 2011). Ug99, which has the designation of TTKSK, is a race of stem rust that is virulent to the majority of wheat varieties. Unlike other rusts, which only partially affect crop yields, stem rust can cause 100% crop loss. Up to 80% of all Asian and African wheat varieties are susceptible to the fungus and according to the FAO, major wheat-producing nations east of Iran -such as Afghanistan, India, Pakistan, Turkmenistan, Uzbekistan and Kazakhstan -should be on high alert (http://www.agro-delo. ru/news/5572.html, accessed 5 March 2008). To combat the menace of rust, screening of various nurseries from national and international breeding programmes was initiated. The aim of this work was to find sources of stem rust resistance and to develop disease-resistant germplasm.Wheat genotypes used in this study included a set of 16 isogenic lines and differentials for Sr genes, 44 wheat commercial cultivars and breeding lines from national breeding programmes of Kazakhstan, Kyrgyzstan, Uzbekistan, Tadjikistan, Azerbijan and Russia, and 170 advanced lines from International Trials Nurseries, CIMMYT.Experimental materials were grown in 2008-2010 in two locations of Central Asia which differed in soil conditions, temperature and moisture. The experimental station in Almalybak, Almaty region, located at the foothill zone is a relatively well irrigated location. Wheat plants were irrigated three times during their development at a rate 600 m 3 /ha. At this location altitude above sea level is 785 m and during the three years of this study annual rainfall ranged from 332 to 644 mm. Nitrogen fertilizer was applied at a rate of 60 kg/ha and phosphate fertilizer at a rate 30 kg/ha. The other location is an irrigated location in Gvardeysky, Research Institute for Biological Safety Problems (RIBSP), situated in the Zhambyl region, Kazakhstan in the desert-steppe zone, and therefore, the climate is extremely dry with great variation. The crop growth season is characterized by drought and dry winds, especially as the crop reaches maturity. The total rainfall is 200-220 mm. Plants were irrigated 3-4 times at a rate 650 m 3 /ha. The soils in both testing locations are light, ranging from sandy losses to brown semi-desert soils to light silt loams. Each experiment consisted of three randomized replications.Field trials were conducted by sowing seed of the entries during autumn (20-25 September) of 2008-2010. The plots were inoculated in the spring at the tillering stage with a mixture of identified isolates representing the most prevailing races of the pathogen from Central Asia.Seedlings of winter wheat cultivars and advanced breeding lines from Kazakhstan and CIMMYT were tested under the controlled greenhouse conditions at RIBSP. The structure of the stem rust population was determined according to the identification system of Roelfs and Martens (1988) based on inoculation of isogenic Sr-lines with P. graminis spores that had been modified. According to this system, the plant reaction is determined on 16 lines divided into 4 groups of 4 lines. The first group included near-isogenic lines for Sr5, Sr21, Sr9e, Sr7b; the second -Sr11, Sr6, Sr8a, Sr9g; the third group -Sr36, Sr9b, Sr30, Sr17. The fourth was the set of near-isogenic lines for Sr24, Sr25, Sr27, Sr32 (Kazakhstani additional set). According to combination of responses of resistant (R) and susceptible (S) plants, each rust isolate was coded in letters. As a result, each pathotype has a code including 4 consonants of English alphabet from B through T. Virulence of the pathotypes was also studied using a set of Sr isogenic lines (Green et al. 1960). Stem rust pathotypes with virulence to 8-13 Sr-genes were used in the greenhouse for wheat seedling test (Table 1). For each plant, infection type (IT), based on a 0-4 scale, was recorded 20 days after inoculation. The IT data of seedling reactions were analyzed using the methods described by Stakman and Levine (1922). Disease severity was recorded following Peterson et al. (1948). Sampling of the spores, their storage, examination, reproduction and use were carried out according to methods described by Kiraly et al. (1970), Konovalova et al. (1977) and Roelfs et al. (1992).The experimental material was screened with the predominant races in the region of Central Asia. Cultivars Bogarnaya 56 and Steklovidnaya 24 were used as susceptible checks, which were used for multiplication of the pathogen spores in the greenhouse and as spreaders in the field tests. Advanced lines of wheat were also tested in the nurseries of CIMMYT-Turkey and at the Plant Breeding Station, Njoro, Kenya.Total genomic DNA was extracted from leaves following the protocol described by Riede and Anderson (1996). PCR was performed as described by Chen et al. (1998). DNA samples from the parents and breeding lines were screened using STS marker Sr24#12, which co-segregates with the Sr24/Lr24 locus (Mago et al. 2005) and is used for identification of wheat lines in breeding populations with resistance to stem rust. PCR products were resolved on 8% polyacrilamide gels stained with ethidium bromide.A total of 11 pathotypes of P. graminis f.sp. tritici were identified from the stem rust samples col-lected in 2008-2009 in Kazakhstan. The study of virulent pathotypes using differentials for Sr-genes indicated that the pathotypes PCP/C and PCR/Q were virulent only to 8 out of 16 Sr-genes studied (Table 2). Isolates of the pathogen studied in 2008-2009 were highly virulent. Isogenic lines of Sr5, Sr11, and Sr25 in 2009 became susceptible to pathotypes TPS/H, TTH/K, TMR/H and TKH/R. Results presented in Table 2 indicate that 6 pathotypes (TDT/H, TPS/H, TTH/K, TKH/R, TKT/C and TFK/R) were highly virulent (part of virulent Sr-lines was 75% and more). They were selected from durum wheat cultivars (Bezenchukskaya 139, Damsinskaya 90 and Navryz 2).The presence of highly virulent pathotypes in the population of stem rust represents a great threat to commercial varieties of wheat in Kazakhstan. The results of tests of wheat cultivars to four pathotypes show that the tested cultivars differ in their resist- Sr5,21,9e,7b,8a,9g,36,9b,30,17,25,32 Sr5,21,9e,7b,11,6,8a,9g,9b,17,25,27,32 TCK/H North of Kazakhstan Sr5,21,9e,7b,9g,9b,30,17,25,32 TFK/R North of Kazakhstan Sr5,21,9e,7b,8a,9g,9b,30,17,24,25,32 Sr5,9e,7b,9g,36,17,24, 25 Sr5,21,9e,7b,11,8a,9g,9b,17,24,25,32 Sr5,21,9e,7b,8a,9g,36,9b,30,17,25,32/Sr11,6,24,27 75.0 Bogarnaya 56 TPS/H Sr5, 21,9e,7b,11,8a,9g,36,9b,30,25,32/Sr6,17,24,27 75.0 Damsinskaya 90 TTH/K Sr5, 21,9e,7b,11,6,8a,9g,9b,17,25,27,32/Sr36,30,24 81.2 Sr5,21,9e,7b,9g,36,30,17,25,32/Sr11,6,8a,9b,24,27 62.5 Sr5,9e,7b,9g,36,30,17,32/Sr21,11,6,8a,9b,24,25,27 50.0 Sr5,9e,7b,9g,36,17,24,25/Sr21,11,6,8a,9b,30,27,32 Sr5, 21, 9e, 7b, 9g, 9b, 30, 17, 25, 32/Sr11, 6, 8a, 36, 24, 27 62.5 К-84482, 18297 TMR/H Sr5, 21, 9e, 7b, 11, 9g, 36, 9b, 17, 25, 32/Sr6, 8a, 30, 24, 27 68.7 Omskaya 19 TKH/R Sr5, 21,9e,7b,11,8a,9g,9b,17,24,25,32/Sr6,36,30,27 75.0 Saratovskaya 29 TKT/C Sr5, 21,9e,7b,6,8a,9g,36,9b,30,17,32/Sr11,24,25,27 75.0 Sr5,21,9e,7b,8a,9g,9b,30,17,24,25,32/Sr11,6,36,27 75.0 ance to disease (Table 3). For example, cultivars Almaly and Tungush were susceptible to all four pathotypes (IT 3, 4, 4+ and Umanka (Russia). The use of these cultivars and advanced lines in the breeding process will allow the development of new wheat cultivars resistant to stem rust.Within greenhouse conditions, 170 F 3 lines carrying Sr-genes were tested for resistance to five races of P. graminis (TDT/H, PCR/Q, TKH/R, TTH/K and TFK/R) that are currently prevalent in Kazakhstan. As a result, 21 CIMMYT lines resistant or moderately resistant to all 5 aggressive pathotypes were identified (Table 4). When testing the resistance of wheat lines to the field collection of P. graminis, lines that were immune or had moderately resistant reactions were identified. Nine wheat lines, which showed '0' infection type (Table 4) in the field, were found to be most resistant to this pathogen. All of the selected lines were highly or moderately resistant to all five pathotypes. A consistent resistant reaction both in the field and in the greenhouse to five specific races was observed in 11 of the F 3 lines. Some of the wheat entries were resistant in the field (IT 0), but were susceptible to individual pathotypes in the greenhouse. However, we also identified a number of lines combining resistance in the field and race-specific resistance to stem rust (T-2003//TREGO/JGR8W/4/ AGRI/NAC//KAUZ/3/1D13.1/MLT, PASTOR/ MILAN/3/F10S-1//STOZHER/KARL, EMB16/ CBRD//CBRD/5/TX69A509-2//BBY2/FOX/3/ PKL70/LIRA/4/YMH/TOB//MCD/3/LIRA and TREGO/BTY SIB//ZARGANA-3/3/TAM200/ KAUZ).Because Ug99 is virulent to the majority of wheat varieties, we tested promising material at the Plant Breeding Station, Njoro, Kenya, 2009. Experimental material tested in Kenya was developed using local spring commercial varieties and wild relatives of wheat. Table 5 presents the resistance of 13 winter wheats to stem and yellow rust. Screening of winter wheat germplasm allowed selection of 6 from these 13 breeding lines which were resistant to both stem and yellow rust: KSI3/97Sr25, 95SR/Progress, 186Al/159 Arthur, Progress/94SR36, 241F4/Tr. monococum CP-1223 and 242T/Tr. timopheevii. Ten of the thirteen lines demonstrated high or moderate levels of resistance to stem rust, among them entries #2070, 2071, 2075, 2080, 2081, 2084, 2085, 2089, 2090 and 2095. At the same time nine of the entries (#2080, 2081, 2084, 2085, 2089, 2090, 2093, 2096 and 2097) showed an immune reaction to yellow rust. Thus, all 13 wheat lines tested at Plant Breeding Station, Njoro are valuable for breeding for both stem and yellow rust resistance.The aim of this part of the study was to screen elite advanced wheat lines from Kazakhstan and CIMMYT with molecular markers linked to stem rust gene Sr24/Lr24. The genotypes analyzed in this study are presented in Table 6. It is known that gene Sr24 is located on the Agropyron elongatum tranlocation on chromosome 3DL where leaf rust resistance gene Lr24 is present (McIntosh et al. 1977). Sequence tagged site (STS) marker Sr24#12 is closely linked to Sr24. This dominant marker typically amplifies only one band, this was used to screen for Sr24/Lr24 genes. The expected size of this marker is 500bp. Resistant germplasm source of Sr24, LcSr24Ag, was positive for the marker (Figure 1). Seven out of twenty five genotypes (#2 Progress/94-W2691SrTt-1-Sr36, BAYRAKTAR and #25 TREGO/BTYSIB//ZAR-GANA-3/3/TAM200/KAUZ) were positive for the Sr24#12 marker and displayed the DNA-fragment associated with Sr24/Lr24 resistance genes. These seven breeding lines are likely to possess Sr24/Lr24 resistance. They also demonstrated immunity or moderate resistance level in the field, except the line #14 with the rating 10MS (Table 5).The screening of winter wheat germplasm from different nurseries in the field and greenhouse allowed the evaluation of resistance to stem rust. From the results obtained in our studies, it can be concluded that the populations of stem rust in Kazakhstan include highly virulent pathotypes. A total of 11 pathotypes of P. graminis f.sp. tritici were identified from the stem rust samples collected in 2008-2009. The pathotypes TDT/H, TPS/H, TTH/K, TKH/R, TKT/C and TFK/R were highly virulent (75% and more). The use of these pathotypes for evaluating wheat germplasm for resistance could help to improve breeding for stem rust resistance.A high level of resistance to all four aggressive pathotypes was observed in wheat cultivars and advanced lines Taza, E-19, E-99, E-102, E-572, E-796, E-809 (Kazakhstan), Ekinchi (Azerbaijan), Dostlik and Ulugbek 600 (Uzbekistan) and Umanka (Russia).Of the 170 advanced lines, 21 CIMMYT lines resistant to 5 aggressive Kazakhstani pathotypes of P. graminis were identified. A consistent resistant reaction to the field collection of rust and to five specific races was identified in eleven advanced lines of wheat. These entries can be used as donors in the breeding programmes aimed at stem rust improvement.Based on the data obtained from Turkey-CIM-MYT and the Plant Breeding Station Njoro, Kenya, out of the 13 tested entries, 6 valuable winter wheat breeding lines resistant to both stem and yellow rust and 10 wheat lines, showing high or moderate levels of resistance to Ug99 were selected.Using the STS molecular marker Sr24#12, associated with Sr24/Lr24, seven carriers of wheat stem rust resistance genes were identified. These results will assist breeders in choosing parents for crossing in developing varieties with desirable levels of stem rust resistance in Kazakhstan and will facilitate the stacking of resistance genes into advanced breeding lines.Thus, this genetic study of stem rust resistance allowed the identification of disease-resistant germplasm of wheat. A number of advanced lines showed a high level of yield potential combined with resistance to the Ug99 stem rust race and to other races which are predominant in the region of Central Asia.  500 bp","tokenCount":"2267"} \ No newline at end of file diff --git a/data/part_6/0293090478.json b/data/part_6/0293090478.json new file mode 100644 index 0000000000000000000000000000000000000000..4ccad22e8b404e45a2415ee3943895baa47fe524 --- /dev/null +++ b/data/part_6/0293090478.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"b6cfb254f96a5bc609cef4b231462cd2","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/71c7f7fb-0377-4996-832b-52d086097ab7/retrieve","id":"1062239457"},"keywords":["Phaseolu","s vulgatis","2","Research networks","3","High-yielding varieties","4","Seed production","5","Soclo-economic development","6","Participatory research","7","Agroindustr:ial complexes","8","Andean Region","l","Voysest","Oswaldo","II","Swiss Agency for Development and Cooperation","N","Title"],"sieverID":"9ad5c130-4cad-4675-a29f-b998252ded9d","pagecount":"73","content":"To increase bean productivíty in the Andean Regían. the Regional Bean Project for the Andean Zone (PROFRIZA. its Spanish acronym) was created through an agreement made between the Government of Switzerland, represented by the Swiss Agency for Development and Cooperation {SDC}. and the Intemational Center for Tropical Agriculture (CIAT). From its beginnings in 1988, PROFRIZA operated as a regional project of CIAT's Bean Program until June 1998, when the SDC began managing PROFRIZA In December 1999. PROFRIZA ceased operating as a regional network. As of 2000, a new strategy will be followed: the SDC's regional offices in Bolivia, Ecuador, and Peru will draw up bilateral agreements with national programs in these countries to carry out activities that will lead to the fulflllment of the project's original mission. CIAT will provide technical assistance.When a mission has the ambitious goal of contributing to the economic, social, and ecological development ojbean-growing areas ojthe Andean Regían, it seems presumptuous to announce that the goal has been accomplíshed: more so when even the most untrained field observer can perceive how backward and deplorable living conditions still are in most rural areas of the Amelicas.We should not forget. however, that the inevitable tasks that we committed ourselves to fulfill had to be accomplished within fixed periods. In the last resort, fulfilling our mission consisted in making contlibutions, fruit of those tasks that we ourselves proposed to carry out within the time peliods agreed upon. The obvious dissatisfaction about how much was not accomplished and the new circumstances that could have arisen in that attempt are the new challenges that will have to be faced under new time periods, and with new strategies and actors.To ensure that the poor find the path to well-being is the work ofmany, each doing his or her part. This report outlines PROFRIZA's participation in that effort. and the catalytic effect its contributions had. The project's most direct contlibution was the development. identification, and dissemination ofimproved germplasmnew varieties that are more efficient in severa! ways. This new technology prometed changes in several areas. even outside the field of production, beca use of the commitment of those institutions leading the bean project in severa! countries.These ins titutions not only achieved in commítting other institutions of diverse interests to participate to va.rying degrees in the projec t. but also s u cceed ed in turning PROFRIZA into a broad~sp ectrum regional network. CIAT's technicaJ assistance and the SDC's financial support were decisive in helping a n ative crop, eaten and valued by the poor. to receive the attention it deserved. but was often denied on behalf of-paradox:ically-demand.The present report outlines PROFRIZA's achievements during 12 years ofwork. years that were decisive for the bean crop's modernization. In the late 1980s, any reference to bean in the Andean Region was commonplace. always alluding to its importance as a \"poor man's food\" and as the exclusive resource of small farmers .Bean is still what was then, an inexpensive protein. a \"meat of the poor~, and the object of many other unsavory expressions. But today it is also a crop that is seeking its own niche in the modero world. The bean enters the new century with a new image in the Andean Regían: as a competitor wíth other products for new openíngs in national and international markets. Bean farmers also have a new mentality; they no longer want to simply grow beans. but they also want to link themselves, somehow, to the commodity market.How did this happen? In this report, we will illustrate PROFRIZA's participation in this process. You will read about how a cardboard box containing lO kg ofb eans and arriving in Bolivia in 1978 from Colombia, could, 20 years later, tum into thousands of tons shipped from Bolivia in metallic contaíners to five different countries. You wíll read how Peru, against all statistical trends at the end of 1980s. converted forecasted deficits into impressive surpluses during the l990s and is today an exporter. You will also find out how Ecuador changed the panora ma for beans in the highlands by introducing bush varleties; and how Colombia compensated its loss in productivity, for explicable reasons, by increasing the area planted. You wíll see how, during this d ecade, societies, fa.nner associations. and new bean programs arase. And, when you finally read how the poor of the Andean Region now no longer suffer from a lack of beans and, moreover. benefit from surpluses, then you will understand why PROFRIZA can say M Mission accornplished!\" Oswaldo Voysest VoysestThe annual growth rate ofproduction dming the 12 years ofthe PROFRIZA project ranged from 2.7% and 4.3%, depending on the country. In the Andean Region overall (i.e. , Bolivia, Colombia, Ecuador, and Peru). this rate reached 15%. Impressive production levels were reached during 1994 to 1996-16.9% for Bolivia, 14.9% for Peru, and 8 . 7% for Colombia, contrasting with the negative val u es for Latin Ame1ica as a whole (-4.5%: Appendix B. Table 27).The growth rate ofbean production during 1988-1999 was higher than that of the population during the same period. In three countries it was even higher than population growth rates during 1964-1975 (compareTables 27, Áppendix B andTable 28, Appendix C).Bolivia is a unique case. Bean production has increased impressively since the 1980s, when the a.rea planted to bean was an insignificant 670 ha, to 1999, when 20.000 ha were planted (Table 6).Because bean ts used as a rotation crop, the increase in area planted to beans is a very important criterion when measuring research advances. Increased area is used as an index of the efficiency of released varieties or of an attractive rnarket.All countries recorded significant increases in the area planted to beans, especially Peru andBolivia durtng 1997-1999 (Appendix B, Table 24). Figures agree with the high growth rates (12.8% and 21.2%, respectively) shown by both countries in the previous 3 years (Appendix B, Table 27).Bolivia hadan important take-offbetween 1994 and 1999 (Table 6); Colombia rnaintained a steady increasing rate throughout the period (Table 18); and Ecuador and Peru maintained relatively stable figures throughout the period (Appendix B, Table 25).When specific regtons are considered, significant increases in productlvity can be observed. For example, in the Peruvian coastal regions, yields of more than 1000 kg/ha were easily recorded (Appendix B, Table 26).Productlvity levels increased by 15% between 1997 and 1999 in the productlon areas of Chuquisaca and Coch~barnba in Bolivia, and, in Santa Cruz, productivity increased by 8% as a result of the use of new bean varieties.New varietles account for most of the progress made. Between 1988 and 1999, countries released 50 new varieties that were disease resistant and had other agronomic advantages such as early maturity, erect growth habit, commercial grain types, and, in the case of climbing beans, less aggressive growth.Bolivia released 8 varietles (Table 10). Colombia 12 (Table 20). Ecuador 14 (Table 17), and Peru 17 (Tables 12 and 13). Between 1996 and 1999 Peru identlfied 12 varietles with export-type grain that were released to farmers (Table 14).Bolivia About 80% offarmers use improved varieties. Durtng 1994-1996, 72% ofbean farmers stopped planting 'SEL 1' in favor of the improved variety Carioca Mairana.Of the area planted to beans in the Santa Cruz Plains, the Chaco Plains, and the northem valleys ofBolivia, 95%, that is, 18,000 ha, are sown with irnproved varieties.Colombia has been characterized as a technology exporting country: 11 varietles, first developed in Colombia, are now planted in 20 countries of Latin Arnerica, North Arnerica, Asia, and Mrica (Table 19).Of the area planted to beans in the Department of Santander, the fourth largest bean-growing region ofthe country, 70%, that is, 5000 ha, are planted to improved, anthracnose-resistant varieties.Of the area planted to bush bean in the northern and southern highlands, 70% (i.e. , 11,000 ha) and 80% (i.e., 1500 ha). respectively, are planted to improved varieties.Once the most serious production problems of coastal areas-rust, viruses, and nematodes-were solved, the dissemination of Peruvian varieties was quick and extensive.In Chincha, the most important bean-growing area of the Peruvian coast, 80% of the area planted to canario-type bean and 100% of the area planted to navy bean are now sown with improved varieties.Along the northern coast, the varieties most used are also improved varieties developed by the project.The varieties developed at the main research sites have been disseminated to neighboring departments (Appendix B. Table 26).In Cusco, 94% of farmers of flve provinces now use new varteties. Production in creases and partlcipation of varieties in this effort are lúgh.In Cusco, new varieties have had a \"colonizing\" effect, spreading to different valleys and neighboring departments (Table 11).In Bolivia, not only 80% of farmers use improved varieties, but 50% of them also use certlfied seed.In Bolivia, 14 farmers have established a small seed company (APROSFYM, its Spanish acronym) that produces certlfied seed, using artisanal methods.In Peru, between 1991 and 1998, a group offarmers, althougll not formally consolidated as an enterprise, produced seed that was used to establish about 5000 ha.In Ecuador, although two groups of seed producers were consolidated in 1994-1996, neither has shown growth durtng the 3 years.Final Report PROFRIZA 1988PROFRIZA -1999 To improve the marketing ofbeans to • Diversify local consumption of bean, favortng the consumer's access to both traditional and other market classes.• Promote the different forros of natural bean consumption (dry and fresh grain, toasted grain, and green pods) and processed (packaged, canned, and frozen).• Convert beans into an export product.During the last decade, annual consumption per capita increased in Colombia, remained stable in Peru, and decreased slightly in Ecuador (Appendix D, Table 29).Both urban and rural consumption figures for Santa Cruz, East Bolivia (Appendix D, Table 31). are impressive, having surpassed even those for Brazil.In Cusca, Peru, the popping bean variety Q'osqo Poroto INIA helped disseminate new ways of ~onsuming this type of bean. In Chincha, Peru, where 90% of the beans consumed bdong to the canario type, navy bean began to be consumed on a mass scaJe, thanks to the dissemination ofthe improved, nematode-resistant 'Larán Mejorado INIA' among farmers.Bean consumption in the Andean Region has become notably diversified (Appendix D, Table 30). representing an important advance because predilectlon for specific and traditional market classes has always been an obstacle for developing the bean trade in the Region.In 1998, Bolivia exported 20,000 tons ofbeans, mainly to Brazil, Japan, and Colombia, for more than 8 million U.S. dollars. Bean has accounted for more than US$36 rnillion in foreign exchange for Bolivia since this crop was introduced into the plains (Table 8). ASOPROF, a small farmers' associatlon, has turned out to be the drtving force behind bean production and export in Bolivia (Table 5).Between 1994 and 1998, Peru exported pulses (cowpea, broad bean, beans, and lima bean, in that arder) for a value totalling more than US$46 million (Appendix E). The evolution of ASOPROF, an orgarúzation created in 1990 with the participation of 11 grassroot organizations, should be highlighted. This asstJciation began with 470 small farmers who planted beans during winter. In 1999. ASOPROF grouped 23 organizations, representing 2000 farmers, and selling beans to Japan. Colombia, Brazil, ltaly, and Spain (Tables 1-5).All national institutions supported by PROFRIZA have received contributions from foreign institutions and, in sorne cases, national institutions. indicating the level of credibillty PROFRIZA has attained and its strength. Especially important is the PROSEM agreement in Peru through which private enterprises (through IPEL members) and the SDC (through PROFRIZA) contributed US$30,000 and US$3,000, respectively, to finance a bean seed production project to be executed by PROMPEX through PROMENESTRAS.The regional exchange of experiences was encouraged between representatives of the most important associations of the Andean Reglan. PROFRIZA extended invitations and sponsored the visits of:The president of IPEL (Peru) to Bolivia on two occasionsThe manager of ASOPROF (Bolivia) to ColombiaThe President and Manager of ASOPROF (Bolivia) to PeruThe Manager of Crop Diversification of FENALCE (Colombia) to BoliviaThe integration so far achieved through the regional network is seen as the first step toward fonning strong national programs.Until 1980, production statistics for the Department of Santa Cruz, East Bolivia, did not include figures for common bean (Phaseolus vulga.ris L.). Twenty years la ter. bean production in the area is sometimes from as muchas 20.000 ha, a stríking fact in a country where bean consumption was traditionally rninimal. Although this number seems insignificant when compared with those of other bean-growing countrtes of Latín America, they are extraordinary when placed in the social context of East Bolivia. The Bolivian case has no parallel in Latin America; the social impact of incorporating beans into the traditional production system is an example of what can be done by a people who want to get ahead with something in which it believes. lt also shows the role that can be played (so that the people may achieve) by agrtcultural research and international cooperation in a special union of interinstitutional efforts led by the local university.In August 1933, thanks toan earthern road that had recently been constructed, the first motorized vehicle entered Santa Cruz from westem Bolivia. Yet. its momentous arrival gave little hint of the great significance that the Cochabamba-santa Cruz Highway, asphalted in 1956, would have in the future of many Bolivians. Foreign missions. arriving in 1942 and 1951, recommended that plantations be modemized and programs of agricultura! settlernent be carried out to relieve pressure in Bolivia's overpopulated interior. In 1953, settlement and exploitation of new areas began. us hering in an era of drama tic changes for the once-desolate vast plains and su b -Andean mountains of East Bolivia.Without analyzing the advantages or disadvantages of this migra tion for the region's economic developrnent versus its ecological balance. the fact is that, b y the beginning of the 1980s, large-scale agriculture had developed in the northern, northeastem. eastem, and southeastem sectors of the Santa Cruz Departrnent, in an approxirnate radius of 160 km from the Santa Cruz City. These areas. where Iand use comprised extensive cultivation of soybean. sugarcane, rnaize, and rice. and livestock raising. are known as ~integrated\". and were joined with other areas expanding under similar progressive technology. While th ese areas grew, areas of settlement (organized and voluntary) expanded. oc:cu pied by migrants from the country's interior (Departments of Cochabamba. Sucre. Potosi. and La Paz). The migrants were attracted by organized plans for settJement or by possibilities of manual labor that th e rice, sugarcane, and cotton farrns offered.The Universidad Autónoma MGabriel René Moreno\" (UAGRM) began work in beans by circumstance. In 1977, the Agricultural Experimental Center (CEA) of the UAGRM's Faculty of Agricultura! Sciences was created, starting up agricultura! research in that center of learning. In 1978, one of its staff, Francisco Kempff, attended the frrst course on beans given by ClAT at its headquarters in Cali, Colombia. In 1979, the CEA became the Institute for Research in Agriculture and Renewable Resources (IlARNR) and, in 1980, the IlARNR's Bean Program was created at Kempffs initiative after his experience in Colombia. The first research activities-of the brand-new Bean Program were to evaluate bean lines from CIAT's international bean yield trial nurseries. Not even the most fertile mind had imagined, when Bolivia received the first cardboard box containing 10 kg ofbean seed from Colombia in October 1978, that, 20 years later, Colombia would be receiving container loads of tons of red beans produced in the plains and valleys of East Bolivia.Bean lines ofblack-seed and Brazilian types , sent by CIAT to the UAGRM, were evaluated during thesis work and in trials in coordination with the MAbapó lzozog\" Project. Other than studying, through thesis work. the most appropriate times for planting and weed control, the Program established, at the opportune moment, demonstration plots in two settlement centers, San Julián and Comando, to help farmers become familiar with the new crop. Kempff had the vision to r ealize the possibilities of beans as a winter crop and, because bean consum ption in Santa Cruz was almost unknown, of selling them to Brazil. Although the small-farming settlers accepted beans, the large-scale farmers in the zones of integration and expansion did not, being reluctant to deal with a new crop and its insecure markets, and the abundant labor required for manually harvesting and threshing the crop. The University and settlers decided to gamble on the new crop. thus beginning their adventure with beans in East Bolivia.The proposal to plant beans as a winter crop, at first sight. seemed simple, so much so that it may even have had occurred to other people who knew the area. The difference, however, was that the UAGRM could effectively implement two basíc measures:• To malee decisions based on research. That is, through research, to determine the answers to questions such as which varieties would be the most efficient, when and where they should be planted, and how to salve the problem of seed supply.• To use a particípatory strategy. Results of agronomic research included the identification of:• 1\\vo recommended sowing dates for beans in the Department of Santa Cruz: winter months in the plains (400-600 m), and summer months in temperate valleys (1600-2000 m).• The best two varleties ofthe black-seeded type-ICA Pijao and BAT 76, which were were used to initiate commercial production-and the best varieties of a differently colored seed for the Brazilian market, particularly 'Carioca' that, since 1983, was called 'SEL 1' and had markedly changed the export panorama.The UAGRM remaíned in charge of the winter production of basíc seed in the plaíns. Certified seed was produced in the temperate valleys, ídentified as the most promising area for seed production beca use of possible planting in the austral summer. Here, the UAGRM directly controlled 40 ha of seedbeds and n umerous small farmers (even as many as 100). who each sowed l to 2 ha. The commercial production ofbeans was. by commission of the CAO, in the hands of the PROMASOR Farmers Association. which negotiated, in 1981, with CORDECRUZ for credit to produce seed and promote the crop. The bean adventure in East Bolivia, thus, hada participatory fo cus from its initial phases.[14Final Report PROFRJZA 1 988-1999Artisanal seed production was begun in the valleys under the responsibility of small farmers. Land was worked with oxen, weeds controlled manually, diseases and pests controlled with manually operated backpack sprayers, and harvesting was manual (by pulling off pods). Threshing was done with chapapas, an artifact similar to a marimba, on which dried plants were placed and gently stru.ck with a flat piece of wood. Seed with a low percentage of broken grains and impurities was thus obtained. Basic seed for planting seedbeds was proportioned out by the UAGRM, once the Regional Seed Certification Office qualified the seed. At harvest, the farmer should have received double the quantity he or she origínally received or, in its absence, the cash equivalent at liquidation. Seed lots were selected by previous agreement among farmers and the Bean Program, and the selection confirmed by the Seed Certification Office. Pesticides were delivered by the UAGRM, at cost, which was charged against the harvest. Duríng the growíng season, the Program gave the certified-seed producers technícal assistance.The mínimum requírements for producing seed encompass a guarantee of the genetic identity of the variety under multiplication, and the compliance with certaín standards of germination and health to guarantee the product's quality.In summary, the bean-seed production system established in Santa Cruz fulfliled the following minimum requirements: •• Planting for seed production occurred ata different season to that for commercial production;• The production was from basic seed; and• The control and supervisory agencies were involved in the process.Only one small detail differentiated the artisanal system from a more elaborate and formal system of seed production: the producers were small farmers who used artisanal practices for bean production and sale. The Bolivian experience was, from the beginning, an authentic process of artisanal seed production, almost without parallel in Latín Ameiica.The Achilles' heel of this excellent planning was in marketing, because production, for lack of domestic markets, was almost exclusively destined for unstable export markets. su eh as those of Brazil, whích were accessed on an informal basis. Usually, black beans were sold or exchanged in Brazil for equipment, machinery, or inputs. Business, however. was not always successful. For example, in Montero, northern Santa Cruz, all the 1500 farmers, who formed part of the Integrated Services Cooperative \"Santa Cruz Norte\" Ltd. (CISSCN). planted black b eans for sale in Brazil. but, on severa] occasions were notable to sell or exchange even one bag. Yet. bean production in East Bolivia grew from almost zero to about 2.000 ha. (IIA-El Vallecito). as the IIARNR carne to be called. Under his direction the Program perhaps made its most important achievements. In 1987, 'SEL 1' ('Carioca'} had already replaced the black-bean type and plans to encourage local consumption were being considered. The CISSCN had ap proved a project financed by USAID to plant 500 ha to cartoca-type beans for export to Brazil and to promote consumption among the 11,000 people who fonned the base of this cooperative of 1500 fanners .Other organizations also developed activ:ities to promote export and consumption of beans. These organízations included an institution whose efforts, together with those of the UAGRM, were key to the development of beans in East BoliVia: the Mennonite Association for Economic Development (MEDA). This NGO was established in the mid-1980s, beginning by entertng the field of promoting the bean crop, giv:ing technical assistance to the fanners of Colonia Berlín and helping to export their black beans to Brazil. In 1987, Colonia Berlín made its flrst legal export of 100 tons to that country with MEDA's assistance. particularly that ofits then Executive Director, Calv:in Miller, who, with Ortubé. was the motor in consolidating bean production in Santa Cruz. The funds that the Swiss Agency for Development and Cooperation (SDC) made available through PROFRIZA to the UAGRM's Bean Program in 1989 were decisive in launch.ing the bean crop in Santa Cruz. The UAGRM could also install demonstration plots precisely where MEDA was organ.izing small farmers and thus con tribute to consolidating the efforts of MEDA's leader Calv:in Miller and lea ders of other groups in organiZing the fanners .Various events highlight PROFRIZA's participation:ASOPROF was created.The area planted to beans gradually grew.A seed production system was consolidated.Both exports and domestic consumption grew.~u -~:} ___ .::=:::..::::::.:...----The \"crisis of fallow la nd\" (described on page 23) was mitigated .Employrn ent was gener ated a nd fa mily welfare improved.The crop expanded t o other areas of the cou n t ry.Useful germplasm was gen erated and dis seminated.Flr\\al Report PROFRJ.ZA 1988-1999 Below we shall see how an activity that began asan adventure becarne reality~ how the bean crop had come to stay in East Bolivia!MEDA's efforts to organize small fanners were rewarded on 16 March 1990. when the National Association of Bean Fanners (ASOPROF) was bom, based on ll grassroot organizations, made up of 470 small fanners who had been planting beans as a winter crop for about 7 or 8 years. The Association currently integrates 22 associations, bringing together 2000 small farmers {Table l). ASOPROF was bom wíth the mission to promote the cultivation and consumption of beans in Bolivia through productlve units based on the small fann. It aims to increase fanner income and improve fanning families' diet. To do so, it prometes the development of the bean crop in new areas where beans can provide an economically profitable altemative for small farmers and be sustainable within their production system. ASOPROF's principal activities are as follows:• Producing and marketing certified bean seed.• Providing technical assistance wíth production and postharvest handling.• Marketing beans on domestic and export markets.• Promoting bean consumption at rural and urban levels .ASOPROF has legal status. Since its foundation, ASOPROF has focused its activities in the Department of Santa Cruz. But, in 1992, its focus widened to include other departmen ts: Cochabamba, Chuquisaca, and Tarija (Table 3). where it operates through the collaboration of other institutions: CEDEAGRO (Cochabamba). FEDEAGRO (Chuquisaca). and ACLO (Tarija).The social unit of ASOPROF is the s mali fanner: almost 65% of the 3500 fanners making up ASOPROF plantan average of 5 hato beans (Table 4).Sorne indicators show the role played by ASOPROF in seed production and marketing and the economic benefits that these have represented for the Association's smali-farmer members (Table 5). So far we ha ve desclibed the bean crop's development in Bolivia since the beginning of the 1980s, but we have not discussed any growth figures . The reader shou ld understand the bean's populality in East Bolivia so that h e or s h e can appreciate the d evelopment of other important acUviUes related to the crop tha t were taking place simultaneously with the crop's development. The fluctuations in the area planted observed between 1983 and 1987 reflect the uncertainty of the Brazilian market. The unexpected growth of area planted in 1991 is the result of the exaggerated expectations of large-scale fanners who, enticed by the Brazilian market prices of the year befare. began producing beans . The sudden. severe shrinking of area planted the next year reflects the deception felt with the same market.The true average of yields was 1000 kg/ha . Yields of 1200 kg/ ha were obtained when the planting areas were relatively small. When more lands were incorporated , particularly of less favorable regions. su eh as the plains. the average yield dropped.Flrlal Report PROFRIZA 1 988•1999 Yields ofless than 1000 kg/ha can be attributed partly to \"bad years\" , affected by climatic variations.At the beginning of the 1980s. once the varieties were identified and basic knowledge of the crop was developed, the UAGRM, under the leadership of Marco Koriyama. began a program for seed production near Mairana (at 1600 m). About 180 small farmers from 20 communities planted about 300 hato beans durtng the austral summer (December-January). This crop was to produce seed for farrners to plant beans as a winter crop (April-May) in the plains (at 400 m).By the begínning of the 1990s. the farmers of Mairana already had a small seed company. Its equipment had been donated by the CIAT Seed Unit, foundation seed by the IIA-El Vallecito, technical assistance by the University and the Regional Seed Certification Office, and administrative and marketing support by ASOPROF. The company was called APROSFYM, and was organized with the participation of 14 farmers. Thanks to the talent of one its members, Tito Orquera. who designed a stationary thresher, APROSFYM succeeded in interesting the FAO-Postharvest Project to help enhance the thresher's model and thus begin producing and selling the machine in series.At the decade's end, the original seed production model is still beíng followed and encompasses not only Mairana and the plains but also otber localities. Research: UAGRM Variety record:Regional Seed Office Producti.on of basic and foundation seed:UAGRM or autborized companies Quality control:Regional Seed Certification Service Thanks to its organized system of seed production, Bolivia can, unlike other countries with older traditions of bean production, exhibit adequate levels of certified-seed production (Table 7).Until 1989, MEDA and CISSCN coordinated the entire chain of activities (distribution of seed, credit, and marketing) ortented toward bean export. From 1990 onward, when ASOPROF was created, the Association assumed these same responsibilities for the small farmers. while PROMASOR & C. A. and two prívate enterplises (DITEX and Cordillera) were in charge of marketing for large-scale To enter the export market and compete with other companies, ASOPROF became partners with MEDA in an export company, ASOMEX. ASOMEX's members include the farmers, MEDA, and representatives of 20 communities. Bolivia currently exports beans to Brazil, Colombia, Japan, ltaly, and Spain. Most of this production comes from small farmers who, 20 years ago. had scarcely heard of beans. The biggest export market is Brazil, which had been accessed on an informal basis since 1980, a little after the bean adventure in Santa Cruz began. In 1986, export to this country was formalized on the signing of the Paz-Sarney bilateral agreement, which ensured a channel for free export for 70,000 t/year. The market begun to grow from lOO met.ric tons in 1987, through 300 in 1988 and 700 in 1989, to 2878 in 1990. The exported beans were mostly carioca type, but other types have also been exported: sorne preto (black) andjalinho (butter). Because of the high number of nisei Brazilans (J apanese descendants) living in J a pan, Bolivia has recently begun exporting carioca-type beans to that country. Through research carried out in recent years by the UAGRM's Bean Program. new bean types have been adapted to East Bolivia, opening up new export m arkets in Colombia (Table 8) and Spain.Pirtal Report PROFRIZA 1988-1999 A 1994 1 study found that ín urban Santa Cruz, 60% of the population consumed camba beans (Vigna unguiculata, or cowpea) at an annual rate af 2.2 kg per ca pita. On introducíng the common bean (Phaseolus vulgaris}. the offer of beans became more diverse. resulting in the new grain becoming incorporated l. Maruique 8, R. 1994. Estudio de consumo y mercado de frejol PhCLSeolu.s uulga.Ii.s L. en el departamento de Santa Cruz [Bolivia). S.S. thesís. University Autonóma \"Gabriel René Moreno\". Santa Cruz. Bolivia.into tbe díet of 53% of consumers, so much so tbat the annual average of consumption rose to 3.9 kg per capita. The introduction of common beans affected tbe consumption of traditional camba beans in that the number of their consumers dropped to 49% of tbe original number and the quantity consumed was reduced by 16%. However, the net balance of consumption of these two grains (Vigna unguiculata and Phaseolus vulgari.s) in crea sed to 160% as the per ca pita bean consumption rose from 2.2 to 5. 7 kg/person.In the rural sector, befare beans were introduced, 37% of tbe rural population consumed cowpea atan annual average rate of 4 kg/person. Now, 70% of tbe population consume botb Vigna and Phaseolus beans. Unlike in the urban sector where consumption dropped, Vigna bean consumption went up by 29%. Total annual consumption of Vigna and Phaseolus beans rose from 4 to 11 kg/person, tbat is, by an increase of 168%.Up-to-date figures from a 1998 study by Norha Ruiz de Londoño, of CIAT, showed impressive bean consumption rates in the vartous social strata of the urban and rural sectors of Santa Cruz:• In the study area, 75% of rural familles, and 50% of urban familles, consumed beans. Consumption rates in low-strata farnilies reached 84%.• Consumption per capita estimated for the rural population surpassed the average for Brazil. In Santa Cruz, annual consumption is 23.5 kg/person (in Brazil, it is 18). In the urban sector, overall consumption is 6 kg, but the population's lower strata annually consume 12 kg/person.• Ifwe consider the average amounts consumed by bean eaters only, then figures are about 31 and 12 kg per capita/year in the rural and urban sectors, respective! y.• Beans contríbute one-third of a rural person's daily protein requírements and 17% of those of an urban dweller's.In Santa Cruz, land grants to settlers varíed from 1 O to 50 ha. Smaller grants were considered as undesirable beca use settlers with only 1 O or 20 ha were found to rapidly exhaust virgin bush and to become caught in the trap of the so-called ~crisis of fallow land\". This crisis sets in when secondary vegetation invades the land left under fallow and settlers find it impossible to combat it. They then have only two options: to use machinery or to raise cattle to take advantage of tbe grass that eventually invades everything. Because both options represent heavy investments, the small farmer must abandon his land and look for more virgin forest. In many cases, receiving more land only meant postponing the crisis that inevitably carne. Fallowing. a widespread in subsistence agricuJture, is nota practice opposed to modern agricultural systems; it can b e improved with the inclusion of crop rotation. alley cropping, plant cover. or a groforestal.[34Final Report PROF'RIZA 1988-1999 For the Santa Cruz settlers, the lack of a sllitable winter crop motivated most to migra te in search of temporary jobs. The introduction of beans into the region allowed settlers to farm their fields in winter and to introduce highly recommended practices such as rotating cereals (maize in summer) with legumes (beans in winter). Beans also enabled fanners to develop capital that would one day allow them to mechanize their property, buy livestock, or do a combination ofboth.Although introducing beans reduced the problems of the so-called \"crisis of fallow Iand\" for farmers, it probably also induced them to incorporate more land under agriculture-beans being profitable and solving a series of daily problems. often far removed from production, but intensely united to their well-being. For example:• In winter, 86o/o of the are a cultivated is under beans. Befare the crop was introduced, only 9% of the arable land was exploited in winter.• Beans ensure better land use: cereal rotation with legumes.• By hampering weed invasion during winter. beans are estimated to reduce production costs of summer crops by as much as US$1.5 million.• Beans elimina te farmers' need to migrate to other regions to find winter work by generating a source of work for the farmers and their families. Almost half of the workdays expended on bean cultivation are contributed by the family. representing about 222,500 workdays for the season. Table 9 shows the growing social impact that beans have on the region as an employment generator.In Santa Cruz, in the areas of integration and expansion where modero agriculture is carrted out. the felling of trees has been pitiless and irrational. In the land settlement areas. increased number of trees were felled in favor of a profitable crop that solved many of the farmers' problems. including those unrelated to production. Farmers of the land settlement areas are part of a tragic scenario: they come from the high plateau, fleeing from misery and desperation, to \"invade\" a piece oftheir own country, covered by jungle. They discover the land and cultivate it, thus offering their children food, stability, farnily identity, and improved well-being. To their country, and to the world in general, they show that poverty such as that which they suffered in the Platea u can be surpassed, that they can always help themselves, provided they have the resources and can be supported when these faíl.The impact of farmers' actions in this case should also include improved living conditions that give their lives more dignity. Hence, when judging the role beans play in East Bolivia, environmentallosses should be balanced against the gains of these human nuclei in terms of growth as people. Man is part of the environment and his well-being is the best example of sustainability-what better example than precisely this one of the human condition!As we have already shown, beans initiated their pílgrimage to Bolivia in 1982, being frrst planted in the Department of Santa Cruz in the plains (at <500 m) as a winter crop and in the mesothennic valleys (1500-2000 m) as a summer crop. Between 1990 and 1998, beans became consolidated in localities other than Santa Cruz: Muyupampa (province ofLuis Calvo) and Monteagudo (province ofHemando Siles) in the Department of Chuquisaca, and the area of Mizque (province of Mizque) in the Department of Cochabamba. In 1999, the bean began entertng the valleys of the Departments ofTarija and Potosí. What a settlerlThose lines and varieties that CIAT introduced to Bolivia in 1979 began to yield between 1982 and 1985 with the fust commercial planting ofblack-seeded varieties: BAT 76, a line from ClAT, and ICA Pijao, a Colombian variety. Varteties of other seed colors then followed, such as the Brazilian Cartoca, Cartoca 80. Catu, Aroana, Ayso, and Rosinha. Ofthese, the most outstandingwas 'Carioca•, which had started, in 1983, with the name \"SEL 1\" and began to predorninate. 'SEL 1' had the virtue of encouraging bean consumption in East Bolivia where black beans had been rejected. In 1990. when Bolivia became a member of PROFRIZA, the flow of improved gennplasm became more intense. The UAGRM's Bean Program strengthened its genetic improvement team, and new market classes of beans Qalinho, calima, cranberry, navy) were incorporated into the Program's work portfolio and new cultivars were launched (Table lO).As a result of the development and dissemination of improved germplasm by the UAGRM, beans not only settled in Santa Cruz, solving the problems that small farmers had with winter. but were also disseminated to other regions of Bolivia to which they brought important changes. These changes functioned (without indulging in hyperbole) as a springboard for small fanners to modem agriculture, as• shown by the farmers having:Pina1 Report PROFRJZA 1988PROFRJZA -1999 • Made changes in the structure of their production system by introducing a new ero p.• Adopted new technology, which had required training.• Developed a capacíty for management and thus could enter export markets.• Achieved access to resources, including credit for working capital, machinery. and other equípment.Although the bean adventure in East Bolivia has many participants, we must recognize that, without the UAGRM's cooperation and guidance, the adventure would not have begun when it did, nor would it have arrived so far. The creative and bold step that the University took beyond the academic and scientific to push ahead a socioeconomic project based on an unknown crop, ridiculed as a food for the poor, is worth pointing out. Local institutions also deserve credit for their altruistic response to the UAGRM's call to support a project aimed at society's general well-being. All benefited from those who dared to dream, and CIAT and the Swiss Agency for Cooperation in Development did well to have believed in them for over 20 years. The bean adventure in Bolivia is an example of collaborative spirit and steadfastness.Working Together.The Hazards of Forecasting ln late 1989, outstanding CIAT professionals made an in-depth analysis of important FAO data and reached the following conclusions regarding the panorama of beans in Latín America for year 2000: y Although the projectiDn of current aggregate productiDn and consumptiDn trends do not poin.t toward a deficit in the supply of beans for year 2000, the level of aggregation conceals the fact that projectiDns for Brazil and the Andean Region establish annual de.ficits of 351 and 107 thousand tons, respective/y. The estimated dejicitfor the Andean Region amounts to 34% of the productiDn, and is mainly concentrated in Peru and Ecuador. •'2 FAO statistics for 1988FAO statistics for -1999 indicate that annual growth rates of bean production, area harvested, and yield in Peru were 4.3%. 2.2%, and 2.0%, respectively (AppendiX B.'Table 27). thus reversing the pessimistic forecasts. Peru is currently self-sufficient in bean production and even exports beans . How did this happen? All can be attributed to govemmental support. a dynamic and motivated prívate sector formed by intrepid entrepreneurs. and small and medium-scale fanners who were confident about the future.In Peru, 97% of alllandowners are small fanners. Here, as in the rest of the Andean Region, bean is not only a crop for low-income fanners, but it is also planted in small areas scattered throughout the country. This crop is planted in a diversity of environments, found in highlands and coasts, ranging from sea level to 3400 m , and under different production systems. In both regions. beans are not only an important component of the local diet, but also an important source of in come for the rural population. But. beca use the conditions of production differ markedly between the two regions, their strategies for tackling bean problems also differ significantly.Peru has about 80,000 ha planted to beans. About 48% of this area is in the highlands (700-3200 m above sea level) . accounting for 45% of the national Pilla! Report PROFRlZA 1988PROFRlZA -1999 production. In this regían, climbing bean, planted in association with maize, predomina tes; 90% of the area is planted under dryland conditions and yield averages are less than 600 kg/ha.Fanners search for climbing-bean varieties with nonaggressive growth, so they can be grown in association with maize in production systems where marketing the ear of tender maize (choclo) is the main objective. The release in 1989 of 'Kori Inti', an early maturing variety that develops in the lower two-thirds of the maiZe crop -a characteristic that prevents lodging-fulfilled this objective. 'Kori Inti' has successfully spread throughout Valle Sagrado in Cusca. and is planted in association with the economically important, Iarge-grained, white Urubamba maiZe.Farmers need disease-resistant, climbing-bean varieties to reduce pesticide use and to express genotypic yield potentiaJ. Examples of the advances PROFRIZA has made toward fulfilling this need are the varteties INIAA Cajabamba, a white bean resistant to rust and anthracnose, and Gloriabamba, a rnesoamerican type resistant to anthracnose-both for the northern highlands . Another variety. Q'osqo Poroto INIA a type of popping bean resistant to anthracnose, was developed for the southem highlands.Farmers also need bush bean varieties with different planting times to expand the crop's cultivation frontier. Examples of such varieties are Jacinto INIA, a bush varlety resistant to three diseases. including anthracnose and adapted to a broad range of altitudes (1600-3000 m.a.s.l.). and INIA 17, another bush variety that is broadly adapted (Table 12)This region accounts for 16% of the country's bean-growing area and 23% of production. Along the coast, all cultivated areas, regardless of crop, are under regulated irrigation. Average bean productiVity ranges from 850 to 1200 kg/ha. 13).Ex:port-type varieties aJso need to be identified that can be successfully planted on the coast. Twelve varieties of the following market classes were selected and disseminated: caballero, Great Northem, navy, alubia, white kidney, dark red kidney, light red kidney, red marrow, pinto, carioca, and black turtle (Table 14).In Peru, to talk about Andean crops is to taJk about patato, an endless number of tubers. quinoa. kiwicha, maize. wheat. barley. maca. and. if need be. broad bean and lentil. but cert.ainly not beans. The paradigm changed slightly as the century drew to an end. and bean became categortzed as an Andean crop beca use of the advances this crop has made, especially in the southem highlands. Among the achievements are the following:Better Companions for Maize 'Q'ello Poroto', a climbing bean with yellow, giant-sized grains. is the most popular variety in the southem highlands . Although farmers plant it in association with maize, its growth habit is characterized by copious vegetative production and a long growing period from planting to maturity. These characteristics can make the bean a difficult companion for certain types of maize, íncluding the very popular starchy varieties Blanco Urubamba, grown for its high market value worldwide in the Valle Sagrado de los Incas (Cusca}. and 'Amarillo Oro'.'Koii Inti', an early maturtng climbing bean with a well-balanced growth habit. can be grown in association with maize without sígnificantly decreasing its yields. Its development has allowed bean production to increase by about 5000 tons of d.ry grain in the inter-Andean valleys of the southem highlands. such as the Valle Sagrado de los Incas, where maize is traditionally monocropped. This bean vartety has spread to Limatambo. Mollepata. Paruro, and Acomayo in the Department of Cusco, and to Curahuasí, Abancay, and Andahuaylas in the Department of Apurimac, al1 in the southern highlands.Another good companion for maize was identified within the class of popping beans, a traditional crop found in the inter-Andean valleys of the Departments of Cajamarca and Cusco. In Cusco. popping beans are grown in association with the maiZe varieties Blanco Urubamba and Amarillo Oro, among others. as a production altemative in whích cultural practices are performed for the main crop (maize). 'Q'osqo Poroto INIA' is a popping bean of nonleafy growth habit and resistant to the main races of anthracnose (Conetotrichwn lindemuthianum) and to halo blight (Pseudomonas syringae pv. phaseolicola). With its release, the cultivation of maize and poppíng beans in association h as íncreased signíficantly in many Cusco valleys situated between 2600 and 2900 m.a.s.l.Popping beans, also called toasting beans. are known in Peru as poroto. ñuña. apa, nwnia, among other denominations. and in Bolivia as k'opuro. These beans constitute a unique genetic resource of the Andean Region, distributed between Cajamarca ln the northem highlands of Peru and the Department of Chuquisaca in Bolivia. In Cusca, popping beans are mainly planted in the hígh valleys of the Urubamba River at sites su ch as Ollantayt.ambo, Pachar, and Urubamba. at altitudes varyi.ng from 2800 to 2900 m.a.s.l.The grains of this type of bean pop when beated in oil or placed in a bot frying pan. These beans presenta broad variability not only in grain color but also in their capacity to pop. Tender, floury t:ypes With high popping capacity are the most ¡]4Flnal Report PROFRIZA 1 988 • J 999 preferred. With low fat and high protein contents, the beans are also used to make a very nourtshing \"ñuña milk\" in sorne parts of Peru.Popping beans not only representan important source of protein for the rural population of the highlands of Peru and Bolivia, but they also ha ve possibilities as a cash crop. Their potential in the urban market is as great as that of peanuts, popcom, and toasted broad beans, all significant snack foods. The popping bean could therefore represent an important source of income for one of the most marginalized sectors of Latin Ame rica: the lndian populations of the high Andes of Peru and Bolivia. Furthermore, popping beans require less fuel for cooking, and, as a result. the consumption of these beans may represent an economic and environmental component in regions where fuel is limited. In addition to its nutritive and fuel-saving attributes, popping beans have a potential for biological N fixation and can adapt to associations with other crops, su eh as maize, making it an efficient food crop. Des pite all these advantages. the popping bean is little known outside its traditional production areas and its cultivation has never reached a Iarge enough scale to plan large-scale marketing because:• Although certain types (e.g., Chec'che, Angel Poroto. and Pava} are preferred, none have been disseminated on a large scale beca use of the wide diversity of consumer preferences.• Most types of popping bean have a leafy foliage that makes them unattractive for planting in association with maíZe varteties of high market value.• Most varteties are susceptible to diseases, especially anthracnose. Ascochyta, and halo blight.• The best known types of popping bean have a varying capacity to pop that seldom reaches 100%, thereby hindeiing marketing.• Most urban consumers have not even heard of popping beans. whereas t-road beans and peanuts are well-known snack foods .An individual selection conducted by Vidal Ortiz in 1991 in a collection from Limatambo, Anta Province. Cusca. was the origin ofwhat later would be known as the vartety Q'osqo Poroto INlA, the first officially released vartety of popping bean. Mirihan Gamarra, who participated in the selection of this vartety, and her team conducted a widespread campaign to disseminate the new variety, showing those majar characteristics that differentiated it from other known types of popping bean:• Nonleafy foliage that allows it to grown in association with rnaize without affecting yields.• Resistance to anthracnose and halo blight. two major diseases.• High market value: lOOo/o popping capacity: the grains, when toasted, do not form rosettes: grains weigh less after toasting than do local ecotypes.'Q'osqo Poroto INJA' is widespread in Cusca. The support of the Peruv:lan Government in setting good prices for popping bean and its inclusion in the National Food Program (PRONAA. its Spanish acronym) were decisive in encouraging plantings of 'Q'osqo Poroto INJA' on a commercial scale in Cusca. Many plantings used the trellis system that allowed yields to reach almost 3000 kg/ha. Although popping beans have not yet entered urban and intemational markets, the availability of a vartety such as 'Q'osqo Poroto INIA' opens up endless possibilities for this ancestral crop. one of the many valuable legacies left by the Incas.Bush beans introduced to Cusca in the 1960s represented an interesting option for farmers because their early maturity meant they could be planted as many as 4 months after the last planting date for climbing beans. This flexibility of planting time allowed farrners to have food practically at any time. Despite these obvious advantages. the area planted to bush beans did not increase substantially and was restricted to a few valleys in Cusca. mainly because the introduced varieties Red Kidney, Puka, and Rojo Mollepata began to show. with time. susceptibility to the main bean diseases occurring in the highlands. Also, the red grain color was not partlcularly popular in the area.In 1980, CIAT supplied a red bean that, despite its color, began to spread throughout the region. In 1995, this bean was released as INIA 17, becoming very popular in the Department of Madre de Dios. deep in the jungle, possibly beca use of its hardiness. The new vartety showed resistance to halo blight and interrnediate resistance to rust, anthracnose, and web blight. In 1994, bean variety Jacinto INIA was released; its golden yellow color was similar to that of the most popular variety. known as Yellow Giant' or 'Q'ello Poroto'. lt rapidly gained popularity among fanners because of its commercially acceptable color, agronomic characteristics. early maturity, and disease resistance.In the southem highlands, new bush bean varieties have hada colonizing effect, spreading throughout several valleys in Cusca, although they are still to achieve large-s cale production in the areas where they are established.In the northern and central highlands. bush beans have also assumed a ucolonizing\" role. Varieties such as Huerequeque INIA, Larán Mejorado INIAA. and Canario 2000 INIAA, d eveloped for coas tal areas. are being cultivated in the secluded inter-Andean valleys (between 800 and 1300 m. a.s .l.) of the Departments of Cajamarca. Ancas h, and Lima.Examples of innovative germplasm for an ancient region-the so-called In ca Region integra ted by the depa rtme nts ofCusco and Apurimac-are new climbing-bean varieties wh ose less aggressive growth h ave made it possible to penetra te areas planted to monocrop ped m aize ; a truly spectac ular popping bean vartety; a nd early ma turing bus h bean varieties that h a ve opened new fron tiers for bean cu ltiva tion .Final Report PROFRJZA 1988•1999 The advantages that this technology has brought to this area is reflected partly in fue progressive increase in the area planted to beans in this regían, being sometimes, as in Cusca, eight times more than the plantings of the early l980s (Table 11).AJthough the germplasm supplied by INIA, through its association witb CIAT Via PROFRJZA. has had a less dramatic impact, it has also brought benefits to the northem highlands, specifically in Cajamarca. 'INIA Puebla'. an early maturtng var.iety resistant to anthracnose, was widely disseminated among fanners of the bean-growing areas of fue Department of Cajarrnarca, and can be found, even today, in marketplaces, together with 'Glortabamba', anofuer widely accepted vartety that was distributed by CIAT in 1985. An adoption study conducted by CIAT in 1990 in bean-growing areas of Cajamarca determined fuat, 3 years after release, 65% of farmers were growing the vartety Glortabamba. About 35% of the bean-growing area showed a 90% increase in productivity, compared with areas growing local varteties. The adoption of 'Gloiiabamba' mean t. in 1988, a 27% increase in production for fue study areas Chota, Santa, and Cajabamba, and a 22% increase for the entire bepartment of Cajamarca. The additional production was estimated at 3038 tons at a value ofUS$1,519,000. The rate ofretum on the research was estimated at 29%, With a cost-benefit ratio of 3.17.Table 12 shows the vaiieties released by INIA for fue highlands overall during the period of PROFRlZA's intemational cooperation actiVities.We could say that by fue end of 1995. fue nomenclature of beans along fue PeruVian coast was limited to such terms as ~canario\", \"bayo\", \"panamito\", \"caballero\" when referring to the most popular beans in the region. In 1999, bean-related vocabulary had expanded to \"cranberry\", \"pinto\", \"caiioca\", \"alubia\", \"navy\", \"calima\", \"red kidney\", \"caraota\", and others that identify intemationally recognized market classes of bean. This enrichment in the vocabulary of farrners and entrepreneurs. and even con sumers. can be attrtbuted to changes in the production s tructure. technology, management. and access to resources. These changes have occurred as a result of a new economic model now being applied in Peru and which opens new opportunities for small farrners to compete in a market economy. Thanks to the technologies developed. beans have b ecome a competitive crop in both domestic and foreign markets.The climate prevailing in the Peruvian coastal region favor s bean cultivation aJI year and is restricted only by water availability for irrigation. La.nd and water are both scarce and expensive; farmers therefore prefer highly profitable crops . However. bean has aJways been an ideal rotational crop in this artd region beca use of its low production costs. rapid economic retums. and high domestic demand. So that the bean can fulfill íts role efficie ntly. ít must not only be resistant to rust. common mosaic. root r ot. and nematodes. but also be early maturtng to cover the temporary spaces left by crops for the international market. A major INIA achievement was the release to farmers of varteties. resista.nt to rust and common mosaic. of alllocaJly consumed m arket classes (Table 13). These varíeties were developed by Angel Valladolid. and are the mos t preferred by farmers . Estimates indicate that they occupy no less than 50% of the area planted to beans along the Peruvian coast. Fína1 Report PROF'RIZA 1988-1999 under the sponsorship of the private sector. PRO MENESTRAS, headed by Valladolid and with CIAT's assistance, initially identified export-type bean varieties that best adapt to coastaJ conditions. Priva te enterprise, through the Association of Exporters (ADEX. its Spanish acronym). also implemented a proposal of productive alliances. through which agroindustries contracted small fanners to plant legumes, guaranteeing their purchase while providing technical assistance, seed, and direct credit. The program's success can be attributed to the crop's short vegetative period (from 90 to 120 days) and the low investments required (US$500-600/ha) . By late 1999, not only had efficient bean varieties of severa! intemational market classes been identified (Table 14), but Peru had succeeded in exporting beans to at least 15 countries ata value of more than US$45 million, during the last 5 years (Table 15). Although this figure may not seem spectacular. it is significant ifwe consider that, only a few years ago, economists had predicted a dire panorama for bean production in this country.Beans is basically a small-farmer crop. The average fann size ranges from 3 to 5 ha in the highlands and from 3 to 1 O ha on the coast. The are a under bean cultivation is estimated to be less than 30% of the fann area. If 73,000 ha are planted to bean in Peru, then 19.000 farmers or rural families produce beans. ofwhich 5000 were in PROFRlZA's area of influence. On the average, l ha of beans requires 60 working days from planting to harvest. most of which involve family labor. The use of improved varieties has increased fanners' income b y more than 30% because of their greater yield potential and lower production costs resulting from reduced use of pesticides. Improved varieties have also helped protect the environment by reducing the need for potentially polluting agrochemicals.In the past. the bean offer showed strong seasonality, adversely affecting prices because of simultaneous harvests in highland and coastal regions. By planting improved varieties with broader adaptation and thu s extending harvest time, seasonality of the bean offer is n o longer so marked. and prices are more stable, thus benefiting farmers . Increased production and productivity have generated new and majar sources of employment throughout the production and marketing chain.Beans as a Small Farmer' s Crop Of all the legumes cultivated in Ecuador, beans are the most widely grown and consumed. Dry grain consumption is as important as that of fresh grain: the national demand for dry grain in 1999 was estimated at 21,670 tons and that for fresh grain at 27,798 tons. The consumption of snap beans (green pods) is also ímportant, although volumes are unknown. Table 16 presents several characteristics of bean cultivation in Ecuador.In Ecuador. 90% of the area planted to beans is located in those altitudes where most Peruvians live and where most of the nation's crops are grown. maínly by small farmers. More than 40% of the bean-growing area is planted to climbing bean in association with maize, a production system that is used almost exclusively by small farmers. Areas producing bush beans are mostly located in highland valleys (1000 tq 2500 m .a.s.l.} or in the foothills of the western cordillera (800 to 1200 m.a.s.l.}. This panorama shows that beans is a small-farmer crop, planted in a region where farmer-market linkages are precarious and where agroindustries do not provide support or advisory services for bean farmers. Most of the produce is destined for household consurnption or the local market. except for beans produced in Carchi, Imbabura, Pichincha, and Chimborazo, departments that, each year. informally export to Colombia the product from about 29,000 ha planted to red beans. (]6Final Report PROFRIZA 1988PROFRIZA -1999 with evident advantages over traditional ecotypes planted by farmers_ As a result, INIAP 403-Bolón Bayo (1988) andINIAP 416-Canario (1994) were released_Select early maturing types. Climbing bean is planted in association with maize, but in traditional sweet-maize-growing areas or where prices for sweet maize justify cultivation. These early matuiing climbing beans must have a harvest time that coincides with the Iipeníng of the comcob. As a result. the early maturing varieties INIAP 400 (1988) andINIAP 421-Bolívar (1999) were developed.Select export-type varieties_ While farmers of the northem highlands. who have access to calima-type (red mottled) varteties, can export their product to Colombia, farmers planting climbing beans could not export their produce. Now, thanks to variety INIAP 412-TOA (1993), they can do so.Conduct improvement by gamete selection, using molecular markers (1998). INIAP undertook the genetic improvement of the two rnost popular traditional bean varieties 'Canario' and 'Bolón Bajo', using modem breeding techniques. INlAP personnel were traíned for this work, and additional resources and technical assistance were obtaíned frorn PROFRlZA and CIAT, respectively.In Ecuador. bean cultivation generates employrnent for both rnen and women. For each hectare planted to beans, about 50 casual working days are needed. Women participate in probably 50% of the tasks for production (weeding and hilling) and postharvest handling (grain dryíng and classification) _ Bush beans are the most important option in production systems of the Ecuadorean highlands beca use of their short growth cycle, ease of plantíng, market demand, and prices. About 50,000 farnilies produce bush beans. As a result of INIAP's patient work during 25 years, Ecuador is now self-sufficient in beans and even exports_ Thanks to the red-grained varieties that INIAP has released to farmers. about 8500 farnilies of the northem provinces of Carchi and Imbabura export from 15.000 to 20,000 tons ofbeans per year to Colombia, representing a foreign exchange of between 10 and 15 million u :s. dollars for the country.In the southem highlands, most of the beans produced are consumed fresh (fre sh grain or green pods). The dissemination of disease-resistant varieties a nd their broad range of adaption can be considered as significant contributions from INIAP, considering that the use of chemical products to control diseases in crops consumed fresh represents a high risk for human health. M u eh remains to be done in the Ecuadorean bean sector. The future will hopefully increase the number of actors. A work team at INIAP is proactive in the search for solutions to problems that are likely to artse in the future so that beans will continue to be served on the tables of Ecuador's new generations. INIAP's work over 2 decades has also included other crops such as peas. bread bean, lentils. and Jupine , and has assigned them priortty (ata level not seen elsewhere) so Ecuadoreans can ha ve access to cheap sources of protein. Based on these results. INIAP can be seen asan effkient custodian of Ecuador's food secmity.In Colo1nbia Sharing is a Two-Way RoadStatistics show that Colombia is a large producer and consumer of beans. However, this production is not enough, and Colombia has to import beans. Another aspect that statistics show is a sustained growth in production and productivity in the country, which reflects. in part, the contribution of technology. However, the reduction in area planted durtng the last 3 years is cause for concem and reflects the overall detertoration observed lately in Colombian fields because of the evident lack of incentives to plant traditional crops (Table 18).Colombia has a longstanding tradition ofbean production and consumption and a potential capacity to absorb greater amounts of beans than it now produces. Bolivia and Ecuador share part of their bean production with Colombia, a current importer. But sharing is a two-way road and what statistics do not show is the enormous contrtbution that Colombia has made to the bean crop, not only in Andean countrtes but also worldwide. Table 19 shows the different places in the world where Colombian varieties, developed by CORPOICA, are grown.Other than having contrtbuted 11 of its varieties so that 20 other countrtes could have improved germplasm for irnmediate use. Colombia has also contributed improved genetic m aterials so plant breeders around the world could use them in their own breeding programs. \"La Selva\" Research Center (2200 m.a.s.l.}, Department of Antioquia, developed, with support from PROFRlZA, populations dertved from interspecific hybrtdization between cornmon bean (Phaseolus vulgaris L.) and so-called petaco bean (Phaseolus polyanthus L.) . Petaca bean is resistant to the fungus Phoma exigua var. diversispora. a characteristic that was transmitted to common bean in a congruent backcrossing program. The matertals generated by this program were made available to the national prograrns in the region. This contrtbution is importan t. given the lack of high levels of resistance to Phoma in common bean. In addition. the Obonuco Research Center (2710 m.a.s.l.) distributed 68 climbing-bean populations that had showed resistance to rust and anthracnose and high yield under Colombian conditions to national programs of the Andean Region. This way CORPOlCA has remained faithful to its tradition of being not only an important center for generating valuable gennplasm, but also a generous donor of its resources .For more than 40 years, CORPOICA during its vartous stages (as DIA. ICA. and now as CORPOICA) has always fulfilled its rnission of mak.ing new varteties available to farmers according to the existing demand. The years during which PROFRJZA operated were no exception. As indicated in Table 20, between 1988 and 1999, Colombian agiiculture continued to benefit from the contribution of improved germplasm generated through the CORPOICA-CIAT agreernent.The greatest virtue of this new germplasrn has been its efficiency, that is, its capacity to be used under the different circumstances of a given production system. Beans have as many as eight different growth habits, they can m ature in periods ranging from 75 to almost 300 days , and, in Colombia, grow at altitudes ranging from 800 to almost 3000 m .a.s.l. Plant breeders in Colombia have had to develop genotypes for the different environments and situations in which beans are cultivated, a task at which they have certainly succeeded. []4Final Report PROFRIZA 1988-J 999Associating with High~Value Crops: The Case of Coffee in CaldasCoffee. a crop that is key to the Colombían economy, is highly technified. The coffee-growing area covers 8,500,000 ha, ofwhich 1,010,000 are located on the slopes ofColombia's three majar mountajn ranges, with an annual precipitation of 1000-3000 mm (rajns falling 350-750 times ayear}. Most of the coffee is planted on lands with a 20% to 90% gradient.In coffee-growing areas. beans are socioeconomically significant, constituting not only an important source of proteins for local consumers but also a source of income for small farrners. Of the total domestic production, 80% is consumed in coffee-growing areas, which, however, produce only 15%. An altemative to increase the area planted to beans would be to intercrop with coffee. Todo so, however, requires bean varieties that are not only preferred by consumers, b ut also have growth habits that do not interfere with the rnajn crop--coffee-in any way. These beans should also be disease resistant because coffee crop management in Colombia dispenses with the use of pesticides. Moreover, the bean's vegetative period should be such that harvesting does not interfere with cultural practices íor the coffee crop. In brtef, the coffee-growing regían needs efficient bean varteties.CORPOICA accordíngly developed, with the collaboration of CIAT and under the auspices of PROFRIZA, the bean varteties ICA Cafetero, ICA Caucayá. and ICA Quimba ya. The release of these varteties has increased the area of beans intercropped with coffee in the Department of Viejo Caldas (now the Departments of Caldas. Risaralda, and Quindío}. The new varteties are now being planted in zocas (cleared squares of coffee land) and on land where traditional coffee is being renewed with the new variety Colombia. As a result, the income of coffee growers is diversified , a staple foodstuff is provided for the large floating population that appears during coffee harvest. and farmers can reduce costs by not having to weed between coffee rows.The Department of Santander accounts for 10% of the country's bean production, surpassed only by the Departments of Antioquia (22%). Nariño (14%). and Huila (11 %}. accordíng to 1987-1997 statistics. Within Santander, the provinces of Guanentá and Comuneros account for 50.2% of the Departrnent's bean-growing area, with 7580 ha planted to the crop; 79.6% of Santander's bean production is located in the municipalities of Villanueva. San Gü, Bartchara, and Curiti.Bean is a relatively new crop in Santander. In the early l980s, it emerged asan economic altemative for local families during the crisis suffered with traditional crops such as tobacco, maize, millet, and pita fiber. Cultivation began with radicaltype red-grained variety. Between 1982 and 1992, anthracnose became the most serious factor limiting bean production in Santander. CIAT lines began to be evaluated in 1987 and. in 1994. the first calima-type anthracnose-resistant variety (lCA Guanentá) and in 1997 another radical-type variety. also resistant to anthracnose. was released (CORPOICA Froilán}. Although not officially released, Colombia.s[] 'AFR 166', an experimental CIAT line with radical-type grain that is also resistant to anthracnose, became widely adopted by fanners by 1995, thanks to the efforts of Adrian Maitre.According to impact assessment studies conducted by CORPOICN. 49.4% of fanners adopted vartety ICA Guanentá and 79% the vartety CORPOICA Froilán. Factors contributing to the success of the newly released varieties included participatory research, a technological offer tailored to farmers' needs and expectations, a stable market demand. and the tobacco crisis. This last factor was especially important beca use the economic altemative of planting beans particularly benefited small fanners. who were mostly sharecroppers. The impact assessment study established that profits from the new varieties were highly attractlve to farmers. The monthly profits from varieties Guanentá and Froilán was 8.9% and 12%, respectively, much higher than the opportunity cost for capital in the area. Moreover, the interna! rate of social retum ofbean research in the area was 64%. By year 2000, these new varieties are estimated to genera te clase to 3000 permanentjobs. ofwhich around 600 would be incremental. Final RepoTt PROFR12A 1988-1999 Appendix BBased on FAO data, statistics corresponding to the 12 years ofthe PROFRIZA project (1988)(1989)(1990)(1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999) are compared with those corresponding to the 12 years befare the date on which CIATbegan clistrtbuting improved germplasm (1964)(1965)(1966)(1967)(1968)(1969)(1970)(1971)(1972)(1973)(1974)(1975).Table 23. Bean production in the Andean Region, 1964to 1999. Country 1964-19661973-19751994-19961997 Country 1964-1966 . 1973-1975 1994-1996 1997-1999 (10 9 ","tokenCount":"11214"} \ No newline at end of file diff --git a/data/part_6/0324622639.json b/data/part_6/0324622639.json new file mode 100644 index 0000000000000000000000000000000000000000..c63925d911989919198bf32cf93c3be019602e6c --- /dev/null +++ b/data/part_6/0324622639.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"e49332f1f7ed69e6600083cedb01416d","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/1d55bc91-1e6a-4a76-b6ce-68d63a6f13ab/retrieve","id":"1688399751"},"keywords":[],"sieverID":"44560043-a138-42d6-b96a-506ef21b374d","pagecount":"4","content":"Los recursos genéticos constituyen la base biológica para la seguridad alimentaria mundial y están conformados por la diversidad del material genético que contienen las variedades tradicionales y los cultivares modernos, así como las plantas silvestres afines a las cultivadas. Estos recursos son la materia prima más importantes de los fitomejoradores y el mayor aporte para la producción y diversidad genética que utilizan los agricultores. Constituyen también un depósito de adaptabilidad genética que sirve como garantía ante el peligro representado por los cambios medioambientales y económicos (1).Se estima que de las 250.000 a 300.000 especies de plantas existentes en el mundo, cerca del 10 a 20% están amenazadas (2). Con los recursos y condiciones actuales, la conservación in situ no permite proteger a todas las especies en peligro de extinción. En todos los países las áreas protegidas abarcan sólo una fracción de los habitats de especies amenazadas, por ello el Convenio sobre la Diversidad Biológica (CDB), suscrito en 1992 por 157 países, reconoce la necesidad de complementar la conservación in situ con medidas de conservación ex situ. En el caso de las especies en alto riesgo de erosión genética o en extinción, la conservación ex situ puede ser la única forma de conservarlas. Para otras especies, la conservación ex situ sirve como una medida complementaria a los métodos de conservación in situ.La conservación ex situ de especies vegetales adquiere cada día más relevancia como parte de una estrategia para conservar la diversidad biológica existente en el mundo. Las actividades agrícola y forestal, así como las ciudades y complejos turísticos están expandiendo aceleradamente sus fronteras, generando degradación de ecosistemas naturales, pérdida de habitats y, como consecuencia, la extinción local de especies. Esto sin contar con otros factores, como la constante degradación por pastoreo y desertificación.Muchos esfuerzos se han concentrado en la conservación ex situ, particularmente los bancos de germoplasma. En 1963, en la Duodécima Sesión de la Conferencia de FAO se promovió fuertemente la conservación ex situ y se marcaron las pautas para la colección, conservación e intercambio de los recursos genéticos. Hoy, como resultado, hay un total de 1300 bancos de germoplasma alrededor del mundo totalizando aproximadamente 6 millones de muestras (3). De ese total alrededor de 600,000 accesiones se mantienen en el sistema del Grupo Consultivo para la Investigación Agrícola Internacional (CGIAR) y el resto permanecen conservados en los bancos de germoplasma nacionales.Los bancos de germoplasma constituyen una de las estrategias más comunes para conservar la diversidad biológica vegetal ex situ, permitiendo conservar por mucho tiempo y en un espacio reducido muestras representativas de diversidad genética de una gran cantidad de especies de plantas (4).Nuevos acuerdos han sido firmados con el Órgano Rector del Tratado Internacional sobre los Recursos Filogenéticos para la Alimentación y la Agricultura, en donde las instituciones se comprometen a proteger las colecciones y hacer que los materiales y su información sea disponible a los usuarios El elemento central del Tratado es el Sistema Multilateral, creado para facilitar el acceso y distribución de beneficios para los 35 cultivos y 29 géneros de forrajes especificados en el anexo 1 del Acuerdo. El acceso facilitado se concede solo para fines de investigación, mejoramiento y capacitación al servicio de la alimentación y la agricultura, los recursos genéticos de estos cultivos son disponibles a través de un Acuerdo normalizado de transferencia de material (SMTA) (5).En 1994, los once centros del CGIAR firmaron un acuerdo con FAO colocando sus recursos genéticos '' en fideicomiso ''. Este acuerdo fue reemplazado por el nuevo acuerdo celebrado el 16 de Octubre de 2006 en donde los Centros Internacionales firmaron un acuerdo de asociación con el Tratado Internacional, mediante el cual colocan sus colecciones dentro del Sistema Multilateral tal como viene indicado en el artículo 15 del tratado (6).A partir de los 70's el Centro Internacional de Agricultura Tropical (CIAT) aceptó el mandato mundial para el germoplasma de fríjol, yuca y forrajes. A partir del 1 ro de Enero de 2007 entró en vigencia para el CIAT, al igual que los otros centros del CGIAR, el SMTA. En el período 1994-1996 se utilizó un ATM CIAT (Acuerdo de Transferencia de Material) y en 1997-2006, se usó un ATM aprobado por la FAO. El CIAT mantiene las colecciones de fríjol y forrajes en banco de semillas y la colección de yuca es mantenida en forma de in vitro. Dentro de los objetivos institucionales del CIAT se encuentran la caracterización, evaluación, documentación, conservación, multiplicación y distribución de los cultivos de su mandato.Estado del germoplasma registrado en el Sistema Multilateral del Tratado Internacional Un total de 64,870 accesiones de fríjol, forrajes y yuca han sido registradas en el Sistema Multilateral del Tratado Internacional. Las accesiones de fríjol conservadas en el CIAT está representada por un total de 35,231, la mayor parte de ellas corresponden a P. vulgaris y el resto a P. lunatus, P. coccineus, P.dumosus y P. acutifolius. La colección de yuca conservada en forma in vitro comprende un total de 6,499 accesiones, un 85,6% corresponde a M. esculenta y el resto a 33 especies del género Manihot. El germoplasma de forrajes está representado por un total de 23,140 accesiones y comprende 150 géneros con más de 730 especies silvestres (7).Los principios y procedimientos en las que se han enfocado las acciones de la Unidad de Recursos Genéticos (URG) del CIAT son las siguientes:1. Suscribiendo el acuerdo con el Tratado Internacional el 16 de Octubre de 2006, el CIAT se compromete a colocar sus colecciones en fideicomiso dentro del Sistema Multilateral de Acceso facilitado y Distribución de Beneficios. Según los artículos de la parte IV y 15.1 del Tratado, el CIAT se compromete a distribuir materiales en conformidad con las disposiciones del Tratado y mantener éste servicio (Art. 12.3) (6).2. El germoplasma disponible para la distribución, está viable, sano y con características conocidas.3. Copias de seguridad son enviadas a otras instituciones mediante Acuerdos entre las instituciones. 4. Repatriación del germoplasma al país de origen cuando es requerido. 5. investigación enfocada en precisar mejor el objeto de la conservación y en mejorar la eficiencia y confiabilidad de los métodos de conservación.6. Formación de recursos humanos especializados en métodos de conservación.Los recursos genéticos son conservados con el propósito de que puedan ser utilizados en cualquier momento. Muchas de las muestras distribuidas cada año son usadas por el mismo centro, mientras que otro grupo de muestras son distribuidas a instituciones externas.Monitoreos periódicos de viabilidad y sanidad aseguran el mantenimiento de las colecciones en un alto grado de calidad permitiendo por lo tanto una distribución segura. La Unidad de Recursos Genéticos del CIAT ha distribuido en un periodo de 25 años un total de 399,570 muestras de fríjol a 98 países, 83,098 muestras de forrajes a 104 países y 28,590 muestras de yuca a 67 países. De los usuarios son los programas nacionales (NARS) y Universidades los que han recibido un mayor porcentaje las accesiones distribuidas, las estadísticas reflejan que la distribución de estos cultivos es relativamente alta.Un total de 20.000 accesiones de yuca y sus especies silvestres son conservadas ex situ en CIAT, IITA, y programas nacionales en más de 45 países (8). El cultivo de yuca es un buen modelo de estrategias complemen-tarias de conservación y varias opciones de métodos ex situ como colecciones en campo, conservación de semillas, conservación in vitro y crioconservación son disponibles para la conservación de éste recurso genético.El germoplasma de yuca puede ser mantenido en el campo, éste método tiene una ventaja porque permite que los materiales puedan ser evaluados y caracterizados. Una de las desventajas es el espacio que puede ocupar y la presión constante a plagas y enfermedades lo que dificulta por lo tanto la distribución de un material sano.La conservación de semilla sexual puede ofrecer un alto grado de acceso y seguridad a corto y largo plazo. Sin embargo, no es siempre disponible porque muchos genotipos son estériles, esta metodología es recomendada para las especies silvestres porque puede asegurar el mantenimiento de la diversidad genética que se encuentra en ellas, lo que permitiría en el futuro atender la demanda de los mejoradores a fin de obtener variedades con mejores características La conservación in vitro en yuca está bien desarrollada y ha sido aplicada en muchos bancos de germoplasma. La colección de yuca constituida por 6,499 accesiones, procedente de 23 países, es mantenida bajo condiciones de crecimiento lento con una frecuencia de subcultivo de una vez cada 12 meses, éste procedimiento permite una rápida multiplicación para la distribución de germoplasma (9). Nuevas metodologías han sido establecidas para lograr un crecimiento mínimo utilizando inhibidores de etileno lo que ha permitido extender el tiempo de conservación (10). Esta metodología ha servido para tener el duplicado de seguridad en otra institución, permitiendo que la reposición de la misma se realice en un período más largo. La conservación in vitro ha sido establecida a partir de plantas libres de enfermedades producidas a partir de termoterapia y cultivo de meristemas y evaluadas para los diferentes virus de la yuca de importancia cuarentenaria como son el Mosaico Común de la Yuca (CCMV), Virus X de la Yuca (CsXV) y Cuero de Sapo (FSD) (11). Estos procedimientos de limpieza y certificación de la colección han facilitado la distribución de germoplasma hacia otras instituciones, tener un duplicado de seguridad en otra institución y repatriar colecciones a países que han perdido sus materiales por diversos motivos, entre ellos desastres naturales, como ocurrió con materiales de Cuba, Perú, Ecuador y Paraguay.De 1979 al 2006 la Unidad de Recursos Genéticos ha distribuido un total de 28,590 muestras de yuca (5,795 accesiones diferentes). La alta demanda nos indica que éste recurso es de interés para los mejoradores y científicos de 67 países. El principal usuario de éste germoplasma han sido los diferentes proyectos de CIAT quienes han recibido un 64% (18,373 muestras) y las instituciones externas han recibido el 36% (10,217 muestras). Estos resultados sugieren que la demanda por el germoplasma de yuca es sustancial y proviene de un rango amplio de usuarios externos y utilizados para diferentes propósitos. Son los Institutos nacionales y Universidades los que solicitan en una mayor proporción materiales de yuca, siendo agronomía, investigación básica y mejoramiento los propósitos más comunes (Figura 1).Otra de las alternativas para la conservación del germoplasma de yuca es la crioconservación, la cual permite el almacenamiento a largo plazo. La metodología desarrollada consiste en la encapsulación-deshidratación de ápices y posterior inmersión en nitrógeno líquido (12). La técnica permite que la yuca sea conservada por muchos años sin necesidad de un mantenimiento periódico. Esta técnica requiere menos espacio y permite duplicar la colección en otro sitio, en el momento la técnica está siendo evaluada en el 10% de la colección de yuca.Una serie de estudios han sido realizados para analizar el costo de conservar el germoplasma de yuca en campo, in vitro y crioconservado. El costo promedio anual varía considerablemente de acuerdo al método de conservación utilizado (Cuadro 1). Estos costos de conservación son insignificantes comparado con el beneficio potencial que se genera a partir del acceso y del uso continuo de éste germoplasma (13). ","tokenCount":"1838"} \ No newline at end of file diff --git a/data/part_6/0325991625.json b/data/part_6/0325991625.json new file mode 100644 index 0000000000000000000000000000000000000000..70fd9ea3404a0a88fd58f85ae993586f363b82a5 --- /dev/null +++ b/data/part_6/0325991625.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"0532838cc3081f8f8d2a882944423009","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/9b06f6b2-5dbe-4ba8-97e9-75a4bb75858f/retrieve","id":"1914642645"},"keywords":[],"sieverID":"3c399170-bb0b-4e96-a557-f1ff02dfcbd3","pagecount":"4","content":"La importancia de la agrobiodiversidad y la consideración de su valor económico total La biodiversidad agrícola es la base para la supervivencia y bienestar del ser humano. Sin embargo, a pesar de su importancia, la biodiversidad agrícola a nivel de ecosistemas, especies y genética continúa perdiéndose a un ritmo acelerado. Entre los factores causantes se encuentran la sustitución y cambios indiscriminados en los sistemas de producción, cambios en las preferencias de los consumidores, el crecimiento económico y la globalización de los mercados, estrategias desacertadas de marcos regulatorios (incluyendo los subsidios), epidemias, desastres naturales y conflictos civiles.Un limitante crítico en la implementación de estrategias de conservación es que a pesar de que cada vez se reconocen más los beneficios de la biodiversidad agrícola, su valor total a menudo no es considerado completamente por parte de los individuos y la sociedad en general. Esto se debe a que numerosos componentes de la biodiversidad agrícola proporcionan una combinación de beneficios al agricultor (como beneficios privados, por ejemplo, relacionados con la producción de alimentos y fibras) y beneficios a la sociedad en general (como beneficios públicos, por ejemplo, relacionados con la resiliencia del agroecosistema y el mantenimiento de los procesos evolutivos y opciones futuras). Los mercados capturan solamente una parte de este valor económico total, subestimando así el verdadero valor de estos recursos. De esta forma se crean sesgos en contra de las actividades compatibles con la conservación y el uso sostenible de los recursos naturales. Dado que los costos de conservación tienden a ser locales (a nivel de agricultor), mientras que los beneficios tienden a ser regionales, nacionales o incluso mundiales, no se puede esperar que los agricultores de escasos recursos puedan asumir los costos de conservación de los recursos genéticos vegetales y animales (RGVA) meramente para el beneficio de la sociedad en general sin contar con los incentivos adecuados para tal fin. Las gráficas y explicaciones ofrecidas a continuación nos ayudan a comprender el origen de esta situación.Se puede percibir la erosión de la agrobiodiversidad en la sustitución del variado acervo existente de RGVA por un rango más reducido de recursos genéticos especializados mejorados. Esta sustitución se presenta como parte de un proceso de desarrollo agrícola enfocado en la intensificación, es decir, la manipulación de los insumos y los productos generados con miras a incrementar el crecimiento agrícola a corto plazo.Puede esperarse que los RGVA locales muestren un mejor rendimiento que los RGVA mejorados en entornos marginales de producción, y que han sido modificados ligeramente por insumos externos. Con la intensificación agrícola, los RGVA mejorados (desarrollados para lograr rasgos productivos bajo entornos modificados) se hacen más productivos debido a su mayor capacidad de respuesta ante insumos externos, especialmente en áreas favorables en términos de potencial agronómico y acceso al mercado.Como se puede apreciar en la Figura 1, los RGVA locales sobrepasarían el rendimiento de los RGVA mejorados en términos de los ingresos que generan para los agricultores a un nivel determinado de intensidad 1 del sistema de producción , I*(0). Tras alcanzar el nivel I*(0), los agricultores encuentran cada vez más atractivo reemplazar los RGVA locales por los mejorados, ya que la curva de los RGVA Mejorados se encuentra en este momento por encima de la curva de los RGVA Locales. Para convencer a los agricultores de mantener los RGVA locales más allá de este punto, sería necesario un incentivo o pago adecuado para compensar al agricultor por la pérdida asociada a la variedad mejorada que deja de sembrar. Se puede determinar la proporción del incentivo requerido teniendo en cuenta la diferencia observada entre las dos curvas después del punto I* (0).Via dei Tre Denari 472a 00057 Maccarese Roma, Italia Contacto: Adam Drucker a.drucker@cgiar.org ¿Pero qué justificaría la creación de tales incentivos? Acaso estas estrategias de conservación no interferirían con el proceso de crecimiento agrícola y la generación de ingresos? De hecho, existen una serie de razones que sugieren que la sustitución de RGVA locales por RGVA mejorados se está presentando bastante prematuramente. Tal sustitución solamente debería presentarse en niveles superiores de intensificación de las fincas, como lo muestra el punto I*' en la Figura 2. Entre estas razones se encuentran las siguientes:1) Se ignoran los valores asociados con la conservación, no asociados al mercado y/o bienes públicos. Es altamente probable que esto tenga particular relevancia en el caso de la agrobiodiversidad. Las características de los bienes públicos no solamente se limitan a los valores de uso directo asociados con la producción de alimentos y fibras, sino que también incluyen los beneficios privados asociados con el uso de la agrobiodiversidad para minimizar riesgos relacionados con impactos externos, como eventos climáticos extremos, plagas y enfermedades. Sin embargo, a escalas geográficas más extensas, el uso de la agrobiodiversidad también cumple un rol de bien público al apoyar la resiliencia de los agroecosistemas, el mantenimiento de las tradiciones socioculturales, identidades locales y conocimientos tradicionales, al igual que el mantenimiento de los procesos evolutivos, el flujo genético y los valores de opción globales.2) Se puede haber sobreestimado el rendimiento de los RGVA mejorados, por ejemplo, en caso de haber obtenido menos producción en fincas de lo esperado en comparación con los resultados en las estaciones experimentales y la existencia de impactos ambientales no previstos.3) La existencia de subsidios para el uso de RGVA mejorados los hace más atractivos a primera vista. Estos subsidios pueden darse de muchas formas, entre ellas, el libre acceso a semillas mejoradas, subsidios en capital para insumos como fertilizantes o pesticidas, servicios de apoyo gratis o subsidiados, precios de mercado subsidiados.Como resultado los agricultores probablemente se enfrenten a incentivos financieros (es decir, privados) que no corresponden con los valores económicos reales o totales (es decir, los valores públicos que incluyen beneficios y costos no asociados al mercado), de modo que lo que sería el punto de sustitución óptimo a nivel social bien podría estar a la derecha del punto I*. Esto significa que la sustitución actual de RGVA por variedades mejoradas trae como resultado el mantenimiento de una cantidad de agrobiodiversidad inferior a la socialmente óptima. Aunque la distancia precisa entre I* y I*' se podría teóricamente determinar conociendo las elasticidades relativas (inclinaciones) de las curvas de los RGVA locales y mejorados, es posible extraer algunas conclusiones generales prácticas de este sencillo modelo analítico.a. Hacia la izquierda de I* se puede pensar que los agricultores tienen incentivos financieros para no reemplazar los RGVA locales, conservando así aquello que provee altos valores económicos.b. Solamente más allá de I*' , la sustitución de los RGVA locales por RGVA mejorados estaría justificada financiera y económicamente (aunque esto no puede usarse para justificar la sustitución al punto de extinción de RGVA).c. Una sustitución que se presenta entre I* y I*' se asocia con una pérdida sub-óptima de RGVA locales, aunque la sustitución parece financieramente deseable desde la perspectiva privada/del agricultor, no puede estar justificada económicamente desde un punto de vista social. Esto se debe a que la pérdida adicional de los valores no asociados al mercado supera los beneficios de la sustitución.Como se aprecia en la Figura 2, las estrategias de conservación en marcos regulatorios para alcanzar el punto de sustitución óptimo, y por ende el nivel óptimo de servicios de conservación de la agrobiodiversidad, incluirían: (a) la consideración de externalidades negativas y eliminación de subsidios (con el fin de abordar los puntos [2] y [3] anteriores), lo cual movería la curva para los RGVA mejorados abajo hacia la derecha (hasta MEJORADOS'); y (b) en donde existen valores de no mercado y valores públicos significativos de RGVA locales (de acuerdo al punto [1] anterior), se requiere implementar unos mecanismos adicionales para permitir la 'captura' de los valores económicos totales asociados con los RGVA locales de modo que la curva para los RGVA locales se mueva arriba hacia la izquierda (hasta LOCALES').• Desarrollo de mercados de nicho para productos asociados con los RGVA locales • Recompensas tipo PSA (pagos por servicios ambientales) para la utilización en finca de los RGVA, denominados PACS (pagos por servicios de conservación de la agrobiodiversidad).Este último además podría aplicarse para alcanzar I*' aun cuando no se corrijan los puntos (2) y (3), o para motivar a los agricultores a conservar los RGVA locales en posiciones a la derecha de I*' -es decir, con el propósito de evitar pérdidas irreversibles estableciendo Figura 1: Sustitución de la agrobiodiversidad desde el punto de vista de la economía (Perspectiva Financiera/Privada) a otros RGVA amenazados (conocido como efecto de \"fuga\").En este contexto, los esquemas PACS podrían proporcionar unas bases más sólidas y flexibles para las actividades de conservación, y pueden ser más adecuados para asegurar la conservación in situ de poblaciones de RGVA.La relación entre el desarrollo de mercados de nicho y los PACS puede entonces verse como una relación complementaria. De hecho, una estrategia de conservación amplia podría incorporar una combinación de instrumentos para incentivos, y como tal podría combinar el desarrollo de mercados de nicho con esquemas PACS.Se necesita valorar debidamente la biodiversidad agrícola e implementar mecanismos que permitan la \"captura\" de aquellos valores por parte de los agricultores que incurren en los costos de conservación, proporcionándoles un incentivo para conservar aquello que beneficia a la sociedad en general. Esto requiere el desarrollo de métodos económicos adecuados, herramientas de apoyo para la toma de decisiones y estrategias adecuadas de intervención política.Aunque un instrumento potencial para la conservación de la biodiversidad nodomesticada -como \"los PSA\"-ha sido aclamado por algunos observadores como \"tal vez la innovación más promisoria en conservación desde Río 1992\", los esquemas PSA no han abordado hasta la fecha la conservación de la agrobiodiversidad. Al contrario, han mostrado una tendencia a enfocarse en el secuestro y almacenamiento de carbono; protección de la biodiversidad no-domesticada, protección de líneas divisorias de aguas y protección de la estética paisajística.La capacidad de los PSA relacionados con la agrobiodiversidad, denominados esquemas de \"pagos por servicios de conservación de la agrobiodiversidad\" (PACS), para permitir la \"captura\" de los valores públicos de su conservación a nivel del agricultor, creando así incentivos para la conservación de la agrobiodiversidad y apoyando el alivio de la pobreza rural, parece ser por tanto algo que vale la pena explorar.un limitante de sostenibilidad--, en tanto compensen a los agricultores al menos por sus costos de oportunidad 2 de utilizar los RGVA locales.El desarrollo de mercados de productos nicho para productos relacionados con la agrobiodiversidad se promueve cada vez con mayor fuerza como un medio para lograr de manera sostenible la conservación a través de la utilización directa de los recursos a conservar. Estos \"enfoques de conservación a través del desarrollo\" son potencialmente sostenibles, ya que parten de los canales de mercados agrícolas ya existentes, y de esta manera se pueden emplear para generar una fuente sostenible de financiamiento.Sin embargo, cabe recordar que depender exclusivamente del desarrollo de mercados puede ser una estrategia arriesgada para la conservación de un acervo variado de recursos genéticos, especialmente considerando que las condiciones del mercado pueden variar con rapidez y generalmente los consumidores y el agro-negocio tienden a favorecer un grupo reducido de especies/variedades de cultivos o razas animales.Los enfoques basados en cadenas de mercado también pueden requerir unas inversiones iniciales relativamente altas para generar volúmenes apropiados de producto, estando estos volúmenes bastante por encima de los requeridos para lograr unas metas modestas de conservación, y en donde el tener demasiado éxito puede incluso desplazar 2 En este contexto, los costos de oportunidad son los beneficios que se prevén de cultivar RGVA locales en lugar de los RGVA mejorados más atractivos a nivel financiero.Figura 2: Sustitución de la agrobiodiversidad desde el punto de vista de la economía (Perspectiva Económica/Social) ","tokenCount":"1928"} \ No newline at end of file diff --git a/data/part_6/0338969354.json b/data/part_6/0338969354.json new file mode 100644 index 0000000000000000000000000000000000000000..ed8f1f04151a131d75250925530ff3a6a4463756 --- /dev/null +++ b/data/part_6/0338969354.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"26d5d40d7187a9cfb727ac694f9aadca","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/5ecaa316-9660-4763-a42f-d93177f9a2dd/retrieve","id":"263330625"},"keywords":[],"sieverID":"f865ac86-945e-42d8-b785-65335a7be204","pagecount":"2","content":"Since 1998, Vietnam has adopted a series of laws designed to promote a basin--based integrated approach to water management. In 2001, eight \"River Basin Planning Management Boards (RBPMBs)\" have been established in eight basins. The RBPMBs act as coordinating bodies under the responsibility of the Ministry of Agricultural and Rural Development (MARD). However, as it turns out their roles and mandates and their positions in the administrative system of water sector remain ill--defined and their contribution to the improvement of water resources management in the basin practically nil. In December 2008, a new decree on River Basin Management was approved by the Government of Vietnam, including the management of river basins to protect the environment as well as the regulationInternational Forum on Water and Food and distribution of water resources. At present, there are changes underway in the RBO arrangement which would operationalize IWRM in river basin in a more meaningful and practical way. But so far no new RBO arrangement has been executed under this new decree for two major reasons: (1) establishment of new RBOs has run up against the existing administrative system of WRM that resists sharing traditional bureaucratic power; and, (2) the linkage between the model and the existing institutions remains unclear. This case examines the challenges of introducing effective and operational RBOs as innovative institutions in the context of a long existing power distribution in the water bureaucracy that does not yield easily to changes.","tokenCount":"240"} \ No newline at end of file diff --git a/data/part_6/0364539774.json b/data/part_6/0364539774.json new file mode 100644 index 0000000000000000000000000000000000000000..71f894c63498d9c930ba192b3b4be5f664e4194b --- /dev/null +++ b/data/part_6/0364539774.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"b07601ace81ee832da11bd16c4e558d5","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/0016e9ef-1ead-4bad-b8a4-543153ee51d3/retrieve","id":"-1065797170"},"keywords":[],"sieverID":"3457460d-04a9-49a9-895e-2aa7d34f2a94","pagecount":"2","content":"Conducting a survey to assess knowledge, attitude and practice (KAP) in all CRP Livestock study sites in Ethiopia. • Training on rational use of drugs for farmers, extension agents, community animal health workers, veterinarians, and drug providers (prescribers and dispensers) • Determine antimicrobial residue level in milk and meat using validated antibiotic residue test kits and compare with the established tolerance (safe) levels of antibiotic residues for consumers. • Test milk samples for antimicrobial resistanceImproved understanding of use of veterinary drugs in small ruminant production and how producers perceive treatment with antimicrobials • The use of drugs in food-producing animals in Ethiopia has been increasing with improved access to veterinary drug stores. • However, knowledge on how these drugs work and how they should be used to achieve the intended impact is often not passed on to livestock keepers. Wrong use of antimicrobials and other veterinary drugs and non-enforcement of withdrawal periods contribute to the risk of emergence of resistances and may result in residues in food items. Resistances are not only a public health concern, but also contribute to treatment failure in livestock, thus threatening livelihoods. • As the major causes of morbidity and mortality of small ruminants in project sites in Ethiopia are respiratory diseases, use of antimicrobial drugs for treatment is inevitable.SmaRT Ethiopia intervention factsheet 22, May 2017Resource requirements (low to high) Land Water Labour Cash Access to inputs Knowledge and skillsBiruk Alemu, ILRI, b.a.gemeda@cgiar.org; Hiwot Desta, ILRI, h.desta@cgiar.org , Gezahegn Alemayehu, ILRI, gezahegn.alemayehu@cgiar.org Barbara Wieland, ILRI, b.wieland@cgiar.org","tokenCount":"251"} \ No newline at end of file diff --git a/data/part_6/0368965797.json b/data/part_6/0368965797.json new file mode 100644 index 0000000000000000000000000000000000000000..59c27f0bd449ed2759844f409c53b56594244746 --- /dev/null +++ b/data/part_6/0368965797.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"e022f615312fe92ea958db8e9da795b8","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/ceff0306-1d4e-49fd-a9d7-4ec0a75da6d8/retrieve","id":"-967671916"},"keywords":[],"sieverID":"296bcae9-4a0c-4553-bf55-7ec457945f07","pagecount":"8","content":"The IPMS steering committee discussed the draft program of work for the period of April 2008 to March 2009, in a meeting held on March 25 at the recently established Ethiopian Meat and Dairy Technology Institute (EMDTI). The proposed program of work and budget was approved during IPMS Board meeting on May 8 th , 2008. Following the requests from Zones and Woredas adjacent to the project's Pilot Learning Woredas (PLW), specific attention was given to the role of IPMS in facilitating the scaling out of approaches and technologies through capacity development, knowledge management and promotional activities. On April 2 nd -4 th , 2008, IPMS participated in the Ministry of Agriculture and Rural Development (MoARD) Millennium Celebration Exhibition held in Addis Ababa. The project used the opportunity to showcase highlights of the various project components to exhibition visitors including H.E Ato Addisu Legesse, the Deputy Prime Minister and Minister of Agriculture & Rural Development and Dr. Aberra Deressa, State Minister of Agriculture & Rural Development.Honey production is a major source of income for many farmers in Alaba. In the past few years the Woreda Office of Agriculture and Rural Development (WoARD) and its partners have taken considerable measures to introduce modern beehives in the area.IPMS started working on apiculture development in Alaba's Galato and Wanja Peasant Associations (PAs) in 2004. The objective was to increase the effectiveness of ongoing apiculture development efforts. A rapid assessment of apiculture development in the Woreda revealed lack of appropriate knowledge and skills as major constraints inhibiting use of improved beehives. To address these capacity gaps, the following series of interventions were undertaken using a value chain development approach, benefiting thirty farmers organized in six groups. and Beza-Mar Honey Processing Factory in Nazareth to increase their knowledge of modern honey production and processing.Sugarcane is cultivated in different parts of Ethiopia by sugar processing factories and small-scale farmers. Bure Pilot Learning Woreda (PLW) is one of the major sugarcane growing woredas in the northwestern part of Ethiopia.Even though studies have shown the profitability of small scale sugarcane processing and marketing, not much has been done in Bure to develop the sugarcane business. Most sugarcane produced is sold at the local market.The typical consumption of sugarcane involves chewing the stalks to extract the juice.The IPMS project in collaboration with BahirDar Farm Mechanization Research Center introduced a simple hand-operated tool that can be used to extract sugarcane juice. This manual cane crusher (juice maker) was more appropriate considering the electricity scarcity in the Woreda.This simple technology increased the value of sugarcane from 1.50 birr per cane, when sold for chewing, to 8.00 birr when processed and sold as juice.The machine can extract four glasses of juice from an average cane and a glass of cane juice is sold for 2 birr. In addition, this processing has increased the number of sugarcane consumers since it broadened the age groups that can consume sugarcane juice -including older men who previously had trouble chewing the cane to extract the juice. The juice can be served with lemon to add flavor or as jelatin-candy prepared by freezing the juice. Another benefit of this technology is the use of sugarcane processing by-product or bagasse for animal feed and fuel wood.Even though, sugarcane processing is new in Bure, its profitability is likely to encourage other sugarcane growers in the country to adopt this technology. Currently, the project is exploring possibilities of other processed products such as jaggery (brown sugar). IPMS facilitated a credit fund of 51,000 birr to help the farmers establish apiary sites in their backyards. An additional 32,475.00 birr was provided to produce bee colonies. The credit fund was channeled through Menchonone Alaba Farmers Union.In 2007, the number of modern beehives per household among trained farmers ranged from four to ten with an estimated annual income of 900 to 3000 per household. Today almost all members produce bee-forage in their backyard, and informal bee forage seed market has developed among farmers. Their income is expected to increase as the number of beehives and bee forage increases coupled with improved apiculture skill and knowledge gained through the trainings.Future IPMS efforts in these area will focus on production of local exotic forage and bee wax. In 2008, two additional PAs will be targeted to scale out the positive outcome of this intervention. The bee-hive producers will also be supported in improved marketing.Innovative Livestock Development...In the past few months poultry development has received a lot of attention in Dale Pilot Learning Woreda. IPMS in collaboration with the Woreda Office of Agriculture and Rural Development (WoARD) has been actively working to increase production and marketing of poultry in the area.Until recently a government-run poultry farm was the major source of supply for commercial pullets (egg-type chickens) and broilers (meattype chickens). However, since demand was outstripping supply, the Woreda Office of Agriculture & Rural Development (WoOARD) and IPMS introduced a community-based commercial pullet supply system.The program started with training sessions (conducted by WoARD experts) introducing modern poultry technology and poultry vaccination for village-based poultry production. Over 80 women organized in five groups attended the training sessions. These women were trained on chicken rearing, management of the hay brooder, preparation of chicken feed from local resources, and vaccination of chickens. Methods, approaches and technology used to raise day-old chicks to the age of three months when they will be ready to be sold directly to rural and urban egg producers were demonstrated.At the end of the training, each woman was provided with chicken hay brooder, a runner prepared by the local carpenters and 50 day-old pullets with sufficient feed and vaccine against New Castle Disease (NCD). The commercial pullets are known for their high egg production, 260 eggs per year compared to local chicken of 60 eggs per year. IPMS facilitated in input supply credit fund of 136,000 birr which was channelled through SNNPR's Rural Finance Service Administration Office.Currently, the 80 women in five clusters in five kebeles (Soyama, Debub Mesenkela, Weynenata, Debub Mesenkela and Ajawa) are rearing 4,000 chickens with 1% mortality rate at the time of reporting. The breed introduced in the first round were preferred as egg layers. The same system can be used to introduce other breeds such as meat & egg types as the need arises. This intervention helped to create an alternative input supply and in the process practically demonstrated that a market-oriented chicken rearing and egg production business can be a major source of income and empowerment for small-holder women farmers.Vaccine for new pullets Knowledge Dissemination... The IPMS working paper series has been established to share lessons-learned and knowledge gained in the implementation of the project with members of research and development community in Ethiopia and beyond. The following three working papers are the latest additions. Please contact IPMS for copies of these publications. IPMS' intervention in facilitating small ruminant fattening in Goma Pilot Learning Woreda is only a few months old. Even so, 120 farmers (57 of whom were women) have already benefited from this activity. In March 2008, IPMS channeled a credit fund of 233,000 birr through Oromia Credit & Savings Share Company (OCSSCo) to be used by farmers for purchases of sheep for fattening. A loan amount enough to purchase five sheep was given to selected farmers. The project used the following two new approaches to ensure the success of this intervention. Cotton seed meal from an oil factory in the Woreda was introduced as supplementary feed. Previously the seed meal was sold to other distant Woredas since intensive fattening was not practiced in Goma. IPMS, the Woreda Office of Agriculture and Rural Development (WoARD) and OCSSCo made the necessary arrangements to buy and transport 170 quintal of cotton seed meal, enough to fatten 600 sheep over a threemonth period. Target farmers were mobilized to form a community based safety-net (insurance) scheme. The farmers contributed 10 birr per sheep as a group self-insurance fund to cover for accidental animal loss and deposited the money in a joint bank account opened in the credit institute. A committee was formed to administer the 6,000 birr insurance fund and community-based insurance bylaws (in Amharic, English and Oromifa (local language) was endorsed and distributed to the committee members.Innovative Livestock Development... Today, farmers in the area plant cowpea, lablab, and pigeon pea intercropped with sorghum and maize. They retain their own forage seed for planting the following year and FTCs have continued supplying forage seeds to interested farmers. More and more farmers are intercropping forage with sorghum and maizeencouraged by the successful experiences of their colleagues. The economic benefits of legume -cereal intercropping and the relationship between improved forage production and improved milk yield is now well understood by many farmers in the Woreda.Ocssco handing over a bank account book to community farmers insurance committeeLately, more and more farmers in Bure and Alamata Woredas have started short-term fattening of small and large ruminants. This new interest was a result of efforts extended by Woreda Offices of Agriculture and Rural Development (WoARD) and IPMS staff to build fattening knowledge and skills among farmers in selected villages.What is most interesting is that while in most villages farmers organize themselves for collective supply of inputs and output marketing, farmers in one village in Bure district and another one in Alamata district have organized themselves for collective production. This is an interesting positive deviation from the \"traditional wisdom\" which no longer promotes cooperative forms of production. Some probing questions revealed the reasons behind these \"new' cooperatives.A major reason for forming these groups was to share knowledge and skill among themselves. Locating and managing the animals in one place allows them to learn together and share skills and knowledge. They can also arrange for input supply and marketing collectively to reduce transaction costs. This is very much in line with the much quoted farmer field school model, except that those are usually initiated by \"outsiders\" with infusion of high external resources.Another reason mentioned was economies of scale of labor. At this early stage of development, the number of animals per farmer is rather low, while management is relatively labor intensive. By organizing themselves into pairs, and arranging for each pair to take turns in looking after the \"group\" stall fed animals, they reduced their labor input compared to fattening the animals individually. This is an interesting farmer innovation in meeting production challenges and acquiring knowledge and skills for development. How such groups will evolve over time as the number of animals increases remains to be seen.Capacity Building... Thirty experts from Woreda, Zonal and Regional Office/Bureau of Agriculture, Regional Bureau of Finance and Economic Development (BoFED) and Amhara Regional Agriculture Research Institute (ARARI) attended the workshop. More than half of these participants are currently engaged in M&E work in their organizations, and they will be involved in the upcoming IPMS M&E activities. Similar trainings will be conducted in Tigray, Oromia and SNNPR in the coming months of 2008.IPMS conducted an introductory GIS training in Awassa town from May 19 th to 24 th , 2008. The objective of the training was to strengthen the capacity of regional stakeholders in collecting, managing, and outputting spatial data. Similar training workshops were previously conducted in Oromia, Amhara and Tigray Regions. Study tours are effective means of knowledge sharing. Two such tours were recently organized in Fogera Pilot Learning Woreda (PLW). The study tours enabled the participants to witness experiences of other farmers in addressing issues around communal grazing areas infested by Hygrophila (Amicala); horticulture/vegetable storage technique; and proper management of credit funds. The tours were organized for the Woreda Office of Agriculture (WoARD) experts, Woreda and Regional Advisory and Learning Committee (WALC & RALC) members and the woreda farmers.The first study tour was a follow-up to last year's Amicala eradication campaign that resulted in the clearing of 268.5 ha of grazing land in 6 highly infested kebeles within one week. Sixteen farmers, four Development Agents (DAs), and three WoARD experts (livestock, horticulture and land administration) participated in the tour. The group visited an enclosed communal grazing site in Atsbi, onion and potato storage technologies in Alamata and protected hillside in Mersa Woreda Tigray and North Wollo. The participants of the tour learned the benefits of enclosed communal grazing areas and the relevance of regional laws and community collaboration in conserving natural resources.Following the study tour, the participants presented feedback reports to the Woreda officials including the Woreda Justice Office representative, and the Woreda and Kebele Administration delegates. At the end of the workshop Kebele level participatory action plans were prepared and approved.As a result of these efforts and continuous community discussions two Kebeles demarcated their communal grazing areas and prepared management plan and internal bylaws -Shina Kebele 10 ha and Kuhar Michael 5 ha. In support of these Kebeles, the WoARD provided more than 500 kilos of forage seeds to be sown in the enclosed areas.The second study tour was organized for ten RALC and WALC members to share experiences with members of the Ada Union about credit fund management and to explore the possibility of using Geneses Farm as a source for germplasm.Livestock health problem coupled with inefficient veterinary service is a major issue for Mieso Pilot Learning Woreda pastoralists. Until recently this issue was mostly addressed through traditional medicines and in severe cases untrained pastoralists provided veterinary services which usually resulted in unwanted side effects. Livestock disease and subsequent attrition is a major livestock development constraint in the Woreda.To meet this challenge the Woreda Office of Pastoralists and Rural Development (WoPRD) and IPMS engaged in strategic capacity enhancement of the pastoralists and agro-pastoralists in a value chain based livestock development approach by establishing decentralized veterinary service provision schemes and training community based animal health workers or paravets. The main focus of the training was to enhance the capacity of traditional livestock healers in primary animal health care procedures, identifying common diseases and using appropriate treatments. Eighteen traditional livestock healers drawn from nine Peasant Associations were given fifteen days of training from March 24 th to April 7 th , 2008 in Bordode zone. The paravets were linked to drug suppliers in Nazareth and Addis Ababa. As of June 16t h , 2008 the eighteen paravets have officially graduated and started their operation using their own finance as initial investment.This inexpensive and reliable form of veterinary service is already benefiting the remote and widely mobile pastoralists in the area. For example, the paravets conducted CCPP (Contagious Caprine Pleuropneumonia) outbreak assessment from April 7 th to 13 th 2008 and reported their findings to the WoPRD, Hirna Disease Investigation Laboratory, and the Woreda Administration Office. This effort immediately resulted in a vaccination program that involved the WoPRD vet staff and ten of the trained paravets. From May 17 th to 20 th , 6300 heads of cattle in 6 PA's were given CCPP vaccination. ","tokenCount":"2473"} \ No newline at end of file diff --git a/data/part_6/0393326758.json b/data/part_6/0393326758.json new file mode 100644 index 0000000000000000000000000000000000000000..ed21054f70344523709a8c0fa79969fb571c6154 --- /dev/null +++ b/data/part_6/0393326758.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"90de0cb83ca6cc46f3b7e9a188923105","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/6287b22f-44b7-4318-9b38-d927ad2615ff/retrieve","id":"1974247640"},"keywords":["Conservation agriculture","conventional tillage","grain yield","soil moisture content"],"sieverID":"ee4e7790-2b02-4301-a4ff-0d4906456b3d","pagecount":"6","content":"This article focuses on the results from trials developed to monitor the short-term effects of conventionally tilled systems versus CA on soil quality and crop productivity under conditions of the major cropping systems in central, north-central and north-eastern regions of Namibia. Conventional tillage (CT), Minimum tillage (MT), Minimum tillage, mulch (MT-M), Minimum tillage, rotation (MT-R) and Minimum tillage, mulch and rotation (MT-MR) were the primary treatments tested. Significant differences (p≤0.000) among the treatments were observed in the 0-60 cm soil profiles where MT-M plots had the highest soil moisture content (39.8 mm, Standard Error of Mean 0.2815) over the study period. A significant difference (p=0.0206) in grain yield was observed in the second season with CT plots yielding the highest grain yield (3852.3 kg ha -1 , standard error of mean 240.35). Results suggest that CA has the potential to increase water conservation and contribute to reduction of the risk of crop failure. Climate change driven degradation under conventional tillage necessitate alternative sustainable tillage methods. Conservation tillage methods and conservation agricultural practices that minimize soil disturbance while maintaining soil cover need to be adopted more locally as viable alternatives to conventional tillage.It is reported that in eastern and southern Africa, between 10 to 25% of rainwater is lost to runoff, and another 30 to 50% is lost through evaporation from unprotected soil surfaces (Rockström et al., 2001). Purcell et al. (2007) highlighted that soil moisture stress resulting from drought, dry spells and high moisture loss through *Corresponding author. E-mail: Kladialaus@gmail.com.Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License Elevatio (m asl): 964Climate: Hot, semi-arid Annual mean temperature: 21.3°CAnnual mean rainfall: 600-700 mm Soil texture class: Loamy sand to sand Source: L.P Kudumo (2019); After CYMMIT (2016) evaporation is one of the primary limiting factors in crop production as it affects many plant biochemical and physiological processes. Due to climate change, mitigation has become more of a need in agriculture as erratic weather patterns are projected to become increasingly worse (Rowswell and Fairhurst, 2011). Tillage is the preparation of the soil for the production of crops for human consumption, animal feed and/or for the improvement of the soil. It is known to influence soil physical properties such as soil hydraulic properties, change flow path, rate of water infiltration and percolation and the stability of the biotic factors (Dexter, 1988). Tillage methods (Fuentes et al., 2003) and climatic factors, especially rainfall distribution and reliability (Fowler and Rockstrom, 2001) influence available soil moisture which is key for plant growth, development and soil physical properties. Conventional tillage (CT) is the most common practice used among small holder farmers and has been practiced for a long time (Chen et al., 2011). However, CT is reported to be unsustainable over the long term in more intensive production setup as it contributes to inefficient natural resource use, poor soil water retention, soil degradation and global warming (Ferna´ndez et al., 2009). Conservation agriculture (CA), on the other hand is a crop management system based on three principles of minimal soil disturbance, crop rotation or intercropping and permanent soil cover with crop residues or growing plants (Friedrich et al. 2012). CA is a less energy intensive system as compared to CT and can improve crop yields while conserving water/moisture, eliminate organic matter loss, and reduce erosion among others (Dumanski et al., 2006). For sustainable and increased agricultural crop productivity, it is critical that good maintenance and improvement of soil quality is undertaken (Fourie et al., 2007) and thus the conservation of natural resources in recent decades has developed into a key global objective and a major national aim for Namibia as well. The objective of the study was to document and compare the effects of different tillage systems (CT and CA) and the influence of the individual CA principles on soil moisture and crop yield. The hypotheses tested were that (a) CA treatments have significant higher water infiltration and soil moisture content (b) the CA principles (minimum tillage, soil cover and crop rotation or intercropping) have significant influence on soil moisture content eventually leading to greater crop productivity.Liselo Research Station (17.524745°S; 24.238707°E) located in the Zambezi Region of Namibia was the site of the experiment. The station is situated 7 km west of Katima Mulilo, 964m above mean sea level in a hot, sub-humid region with mean annual temperature of 21.3°C and mean annual rainfall of 600-700 mm. The site predominantly has loamy sand to sand with pH of 5.3 (Table 1).The experiment consisted of eight treatments in a Randomized Complete Block Design (RCBD) set-up with four replications on a 2016 m 2 trial plot (50.4m x 48m). Treatments tested were; Conventional tillage only (CP), Convectional Tillage with Mulch (CP-M), Conventional Tillage with Rotation (CP-R), Conventional Tillage with mulch and Rotation (CP-MR), Minimum tillage (MT), Minimum tillage, mulch (MT-M), Minimum tillage, rotation (MT-R) and Minimum tillage, mulch and rotation (MT-MR). Each plot was composed of 7 rows (90 cm row spacing and 35 cm within row) by 12 m and plots with rotation were split into subplots each with 7 rows by 6 m. CT plots were tilled with an animal drawn mouldboard plough, while CA/minimum-tillage plots were tilled with an animal drawn Magoye ripper, opening narrow furrows about 5-10 cm deep.Soil samples were taken at the onset of the study and tested the levels of nitrogen and phosphorous, Organic C, estimated SOM and pH for comparison against suggested nutrient ranges suitable for grains. Soil samples were tested using spectral analysis by a mobile Soil Lab stationed at the Directorate of Agricultural Production, Extension and Engineering Services (DAPEES) office Of the Ministry of Agriculture, Water and Land Reform (MAWLR) of the Republic of Namibia in the town of Rundu in the Kavango East region.Maize (Zea mays, Commercial hybrid maize variety Zamseed 606) was the principal crop and cowpea (Vigna unguiculata L. Walp.) an important secondary crop used in rotation with maize. The maize and cowpea crop varieties were manually seeded in November during both cropping seasons. Maize was seeded in rows spaced 90 cm apart with inter row spacing of 35 cm using two seeds per planting station, later thinned to 1 plant (31,746 plants/ha target Treatments with cowpea rotation were mulched with 2.5-3 t/ha of grass at the onset and all crop residues retained on the soil surface in the subsequent seasons after harvesting. Weed control was achieved by disturbing only the top soil using a hoe at 30 day intervals and when necessary. At harvest, cobs were removed from the plots and crop residues (Stover) was retained on the respective CA and CT treatments.Access tubes were installed in all plots for the purpose of soil moisture data collection, one tube installed per plot and readings taken using a capacitance probe (PR-2 probes, Delta-T Devices Ltd., UK) to the depth of 1 m. Soil moisture measurements were taken once a week during the dry season (May -October) and twice per week during the rainy season (November -April) and calculated as mean soil moisture content in millimeters (mm). Data from the 0-10, 10-20, 20-30, 30-40 and 40-60 cm horizons were recorded over the study period, calculated and presented as mean soil moisture content (mm) in the 0-30cm and 0-60 cm soil depths.Maize was harvested at physiological maturity and total aboveground biomass and grain yield determined on each plot. Subsamples of 20 cobs per plot were taken as samples and used to determine maize grain moisture.Linear model, Analysis of variance (ANOVA) using Statistical analysis software 'Statistix 9' for personal computers was used to test for normality and test for any significant difference in moisture content and grain yield. Probability levels of 0.05 were used to determine the level of significance among the means. LSD All-Pair wise Comparisons Test was used to compare soil moisture and grain yield for treatment effect. The next section presents the results.Soil testing results showed low levels of nitrogen and phosphorous, organic carbon, and estimated SOM, readings far below the suggested range for grains. Only potassium and Soil pH fell within the suggested range for grains (Table 2).Rainfall in the 2016/17 cropping season was erratic with a short rainy season with the site receiving a total of 499.9 mm/a. A two week and six day dry spell was experienced during the first season between February 2nd and 22nd, 2017.In the subsequent season (2017/18), a higher total rainfall of 521 mm/ was recorded although the rainfall events during the season were similarly erratic with especially low rain incidences at the onset of the season (Figure 1). Over the study period, MT-M continuously had the highest soil moisture content, particularly in March (69.0 mm), while MT-MR was almost consistently the least soil water storing treatment over both the rainy and the dry seasons in the two years (Figure 1). Conservation agriculture is reported to be most effective when all its three operational principles are put into practice accompanied by good timing of all operations (ZCATF, 2009). Although all CA principles were applied in MT-MR, the results in terms of soil moisture conservation are at variance with expected impact of this treatment. CA is taken to be a long term intervention, as most benefits especially improvement of the soil's physical properties are though only enjoyed in the longer term (Derpsch, 1999), 4-5 years after application of such soil tillage system (Thierfelder et al., 2015). It is probable that the two years implementation of CA treatments was insufficient to reverse effects of many years of conventional tillage on the land. The fact that MT-MR showed differences from other CA plots could possibly be due to a reduction in soil water holding capacity of nontilled plots induced by openings in the soil left by decaying roots of crops and weeds or the effective extraction, use and evaporation of water by crops in the MT-MR plots.Significant differences (p=0.000) were observed between combinations of tillage systems and CA principles in relation to soil moisture content, in agreement with findings by Fuentes et al. (2003) and Gicheru et al. (2004). Mean soil moisture content ranged from 34.1 to 39.9 mm, with minimum tilled plots higher in moisture content than CT plots. The observed differences may be attributed to the water saving techniques incorporated in the treatments particularly in MT-M (39.8mm) and MT (37.7mm) plots while CT only plots were on the lower end as the second least water conserving treatment (36.1mm). A CA treatment with all principles incorporated, MT-MR, was the least water storing treatment of all plots (Table 1). McVay et al. (2006) and Thierfelder and Wall (2010) reported that conservation agriculture plots generally have greater water content in years with low precipitation, as was recorded in this study carried out in years in which rainfall below the annual mean was recorded. In years of high precipitation, no greater differences are found between CA and CT plots (Thierfelder and Wall, 2010). While it's unexpected, plots where all CA principles are incorporated are sometimes found to retain lower soil moisture content than a field ploughed, not mulched and with no crop rotation. No-till treatments may reduce water-holding capacity leading to reduced moisture (Liu et al., 2013).Maize grain yield in the first season was not significantly affected (p=0.0884) by tillage systems and CA principles, however significant difference (p=0.0206) was recorded in the subsequent season (Table 3). CT-MR plots recorded the highest grain yield with MT being the least productive. Minimum tillage with selective incorporation of CA principles increased maize grain yields. It appeared that incorporation of mulch or rotation to minimum tillage leads to increased maize yields as observed MT to MT-M and MT to MT-R, respectively (Table 3). It was also observed that incorporation of both mulch and rotation led to the highest increase in yield from MT to MT-MR. Maize grain yields were found to follow no particular order over the two seasons. Minimum tillage treatments averaged less than 1500 kg ha -1 maize grain yield in the first season and more than 2500 kg ha -1 in the second season (Figure 2).Although no significant difference (p=0.0884) was observed in the first season, conventional tillage treatments were found to have yielded more maize grain than minimum tillage treatments, indicating the benefit of CT in the first season.Maize grain yield followed the order CT-MR > MT-MR >CT-M > MT-R >CT-R > MT-M >CT>MT in the second season during which soil moisture was not very different between treatments (Figures 1 and 2). Higher soil moisture due to mulch appears to have positively influenced maize grain yield, as seen in the second season, where MT-MR delivered the highest maize grain yield of all the CA treatments and second highest overall in contrast, to the first season. MT-M and MT in that order had higher soil moisture content than conventional tillage treatments, but their maize grain yields were generally lower than that of CT (Figures 1 and 2). Mulched plots, CT-MR, MT-MR, CT-M and MT-M generally had higher maize grain yields than plots not mulched. Thus, whereas MT-MR may have recorded lower soil moisture content relative to all other treatments, this did not translate into lowered maize grain yield. This may point to effective and efficient use of soil moisture by maize under MT-MR where the crop extracted more soil water and converted it into higher yield.The results indicated that minimum tillage systems conserve more soil moisture in the 0-60 cm deep soil profile and can improve maize grain yield as compared to traditional tillage. Even where soil moisture may have not been conserved in CA plots, the grain yield was superior.Generally, CA (Minimum tillage) treatments had higher mean soil moisture as compared to CT (conventional tillage) treatments throughout the study period, especially over dry season.Application of mulch and crop rotation appeared to positively influence both mean soil moisture and maize grain yield over the study period compared to no mulching and, not practicing rotation.Yields followed the order CT-MR > MT-MR >CT-M > MT-M >CT>CT-R >MT-R >MT clearly showing mulch's influence on crop yield. This study has in part shown that reduced soil disturbance and residue mulch application can conserve soil moisture, and when implemented together with crop rotation practices enhance crop performance and improve maize production in the northeastern regions of Namibia.","tokenCount":"2378"} \ No newline at end of file diff --git a/data/part_6/0413801329.json b/data/part_6/0413801329.json new file mode 100644 index 0000000000000000000000000000000000000000..db67e96f006636a4bb43db496ac1972e014de271 --- /dev/null +++ b/data/part_6/0413801329.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"dcd0bebb5dc074e5c4828adb33a49983","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/13658b28-1469-446e-8a5a-d19984d067fb/retrieve","id":"-1817460536"},"keywords":[],"sieverID":"156677d0-c5d8-4799-b858-2d74cb0bdf86","pagecount":"18","content":"Titles in this series aim to disseminate interim climate change, agriculture and food security research and practices and stimulate feedback from the scientific community.This activity was conducted to determine climate-smart agricultural practices' biophysical and socioeconomic impacts over the last 12 months, using the Doyogena (Southern Ethiopia) and Basona (North Ethiopia) climate-smart landscapes as a case study. More specifically, this activity addressed if CSA practices (i) guarantee farmers resilience to climate change; (ii) increase food productivity and household income; and (iii) prevent gender-related bias (i.e., improving women's participation in decisions, access/control over resources). From each site, 200 adopters (i.e., farmers who practicing the abovementioned practices) and 200 non-adopters (i.e., farmers` as usual practices) were selected randomly. Hence, 800 households were surveyed from six villages from Doyogena and 25 villages from Basona sites. The activity was conducted between 21 December 2020 to 05 January 2021 at Doyogena and between February 01 -16, 2021 at Basona climate-smart landscapes. Twelve enumerators for Doyogena and fifteen for Basona sites were selected, trained for three days, and performed pre-testing with 8 -10 farmers before data collection. At Doyogena, a portfolio of eleven promising CSA options was evaluated, namely, (i) terraces coupled with Desho grass (Pennisetum pedicellatum); (ii) controlled grazing; (iii) improved wheat seeds (high yielding, disease-resistant & early maturing); (iv) improved bean seeds (high yielding); (v) improved potato seeds (high yielding, bigger tuber size); (vi) cereal/potatolegume crop rotation (N fixing & non-N fixing); (vii) residue incorporation of wheat or barley; (viii) green manure: vetch and/or lupin during the off-season (N fixing in time); (ix) improved breeds for small ruminants; (x) agroforestry (woody perennials and crops); and (xi) cut and carry for animal feed. At Basona, on the other hand, the impact of seven CSA options was evaluated, namely, (i) terrace (soil bunds); (ii) terraces coupled with phalaris and tree lucerne); (iii) trenches; (iv) enclosure; (v) percolation pits; (vi) check-dams; and (vii) gully rehabilitation.Abebe Nigussie is an assistant professor at Jimma University and a part-time consultant. His research focuses on the biogeochemical cycle of carbon and nutrients from agricultural soils and developing interventions to reduce soil greenhouse gas emissions. Scientific studies have shown that the agricultural sector is being affected by extreme weather events such as droughts, heavy rainfalls and high temperatures (Lesk et al., 2016). The negative impact of climate change is expected to be more severe in developing countries -where food production depends entirely on rainfall (Recha et al., 2017). In Africa, for instance, the yields of maize (Zea mays), sorghum (Sorghum bicolour), and millet (Panicum miliaceum) are expected to reduce by 5%, 14.5% and 9.6%, respectively, due to climate change at the end of 21st century (Knox et al., 2012). Therefore, it is crucial to develop technologies to curb the adverse impact of climate change on food production and realize sustainable development goals aimed to eradicate poverty and hunger by 2030 (Ambaw et al., 2020).Climate-smart agriculture (CSA) practices have been promoted as a prominent strategy to improve farmers resilience to climate change and reduce greenhouse gas (GHG) emissions. Evidence also suggested a significant role of CSA practices to ensure food security under a changing climate. With this premises, the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS) implemented integrated CSA practices in highly degraded landscapes across different developing countries, including at Doyogena (Southern Ethiopia) and Basona (Central Ethiopia). The integrated CSA practices at Doyogena and Basona include soil and water conservation measures, grazing management, crop rotation, incorporation of crop residues and perennial-crop based agroforestry systems. These CSA portfolios are implemented to rehabilitate degraded lands and improve resource-poor farmer resilience to climate change. Therefore, the objective of this study is to assess the perceived effects of CSA practices on biophysical resources, food security, crop and livestock productivity, income and adaptive capacity of smallholder farmers for climate shocks. In addition, this study will examine gender-disaggregated effects of CSA practices on farmers' livelihood and key gender dimensions (i.e., decision making, access over resources). This activity will identify the main climate shocks over the last 12 months and monitor the contribution of CSA options to aid farmers resilience to these climate shocks. A total of 400 farmers from Doyogena and 400 farmers from Basona were selected randomly. In each site, 200 households -who have practised CSA practices (i.e., treatment group) and 200 households -who have not received CSA interventions were used in this study. The control groups are those who live in the nearby climate-smart landscapes. This study approach helps monitor the impacts of CSA options by comparing the treatment groups with the control in terms of livelihood/welfare indicators (i.e., food security, productivity, income and resilience of farmers for climate shocks). Training workshop for data enumerators at DoyogenaPrior to data collection, eleven enumerators for Doyogena and 15 enumerators for Basona were selected.These enumerators are working at the regional agricultural research centre, university and central Accordingly, nine household heads -that were not part of the main survey -were used for pre-testing.On average, enumerators in both districts used to fill in three survey questions per day, resulting in 399 and 396 completed questionnaires from Doyogena and Basona Worena districts, respectively.Some responders were unwilling to give information for some questions, particularly on the household income, production (i.e., yield), the number of livestock owned and land size. In addition, few respondents were unwilling to make themselves available for the interview; in such cases, we replaced them with other household heads with similar household characteristics. Sometimes, the Geofarm tool was not working at all and/or was very slow. Data synchronization takes a very long time, and on average, it took about 15-30 minutes to synchronize the data. At Basona, it wasn't easy to get female respondents.The questions on income and production should be designed so that farmers can respond to the questions honestly. The Geofarm tool needs to be improved for (i) efficient and quick off-line data collection; and(ii) fast data synchronization.This activity report aims to present the steps followed in the data collection process, which will monitor the uptake of CSA practices in the climate-smart villages in the Doyogena and Basona Worena districts.Based on the data collected, the following steps will be:▪ characterization of rural farming systems and livelihoods to determine household incomes, productivity and food availability, indicators of food security and poverty, and farm and household characteristics;▪ Examine the perceived effects of CSA options on farmers' livelihood (agricultural production, income, food security, food diversity and adaptive capacity) and key gender dimensions (participation in decision-making);▪ Provide recommendations that can help donors and policymakers to justify funding and guide priorities in scaling up the adoption of CSA technologies and practices;▪ Produce CCAFS Working paper and info notes; and▪ Produce manuscript and submit to a peer-reviewed journal.","tokenCount":"1116"} \ No newline at end of file diff --git a/data/part_6/0437199466.json b/data/part_6/0437199466.json new file mode 100644 index 0000000000000000000000000000000000000000..bc81325e3fc27217d694ee7d87dbb10c97223d21 --- /dev/null +++ b/data/part_6/0437199466.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"d9087ba6d95b61952572a845d9004255","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/8ca99c27-9916-4346-8f4c-d9cfff0052d1/retrieve","id":"-312581172"},"keywords":["TAAT Clearinghouse. 2022. Small-scale Farm Mechanization Catalogue. Clearinghouse Technical Report Series 015","Technologies for African Agricultural Transformation","Clearinghouse Office","IITA","Cotonou","Benin. 24 pp Small-scale Farm Mechanization Catalogue"],"sieverID":"4d7593fa-89c5-4469-9d4f-7b6777f6eaad","pagecount":"28","content":"Front cover photographic credits: A hand tractor converted into a trailer (Ikonic Farm Machinery (K), safe and effective threshing operations (Sasakawa Africa), a woman learns how to operate a hand tractor (Ikonic Farm Machinery) and the impressive reach of a water gun.The TAAT Top 100 Technologies. The Clearinghouse developed a database of the Top 100 Technologies that are transforming African agriculture. It is based upon the approaches of the TAAT Commodity Compacts but also includes those from the CGIAR Collaborative Research Programs that are recently described as ready for next user. These technologies are divided between those involving improved genetics and plant and animal breeding (23%), those based upon the distribution of digital information (3%), production input products of proven efficacy (21%), crop and animal management technologies of utility within agricultural extension messaging and campaigns (27%) and the availability of appropriately designed labor-saving equipment (26%). These technologies have a direct role towards the achievement of the Sustainable Development Goals in relation to farm productivity, food security and hunger reduction, responsible food consumption, improved household nutrition and diets, economic growth, climate-smart innovation, partnerships for the goals, and improved human equity and empowerment.The Top 8 Mechanization Technologies. This catalogue presents eight technologies that serve to mechanize and automate small-scale farming. These technologies include: 1) Hand tractors for land preparation, 2) Mechanized weeding operations, 3) Power sprayers for agrochemical delivery, 4) Land augers as an innovative labor-saving tool, 5) Drip irrigation for efficient water use, 6) Water guns for cost-effective irrigation, 7) Multi-threshers for efficient post-harvest processing, and 8) Forage choppers in livestock feed systems. Details on each of these eight technologies are included in the catalogue. Also included is information on the safe handling and maintenance of these equipment.Background. A power hand tractor is a small two-wheel device powered by a small petrol or diesel engine ranging in power from 5-18 horsepower. These machines are most often attached to a rotavator that allows for land tillage, but other attachments are available, including those that create furrows. These tractors are guided by handlebars that provide control over their direction and downward force and accompanying throttle to guide engine speed and a release to disengage the engine from the rotovator. The most powerful hand tractors can break new ground and dry heavy soils, while the least powerful ones are best suited to preparing previously cultivated soils. These machines are different that riding tractors with two axils that are generally 50 hp or greater. There are many brands of these two-wheel tractors and reference to them includes terms such as \"rototiller, walking tractor, mechanical ox, and single-axle tractor\".Hand tractors reduce the drudgery of hand tillage, a practice closely associated with the drudgery of small-scale farming. Because of their smaller size and tight turning area, hand tractors till areas that cannot be accessed by larger tractors, including lands with steeper and irregular slopes. Hand tractors are powered by a single axil (gear only), so there are no additional belts or chains, improving their reliability and reducing maintenance requirements. It is unlikely that a farmer managing only one hectare or so can afford a riding tractor, hand tractors available for US $700 to $3,000 are much more within reach. A single operator can prepare a hectare of land in a single day using this machine. Many models have attachable tines that allow for adjustment in cultivation width, allowing for use as a weeder at wider crop spacing. Some tillers can be used in flooded paddy soils as well, allowing for the incorporation of residues and inputs.Maintenance is fairly easy, unlike larger tractors that require skilled mechanics. One must regularly check oil and fuel levels and ensure that dirt does not accumulate on the engine during use. The depth of tillage is less than a riding tractor and these machines may form or prove unable to break through hard pans that form in clayey soils. The times of tillers may become clogged by rocks or soil clods, forcing temporary shut down during safe removal.A 12 hp diesel-powered tiller with adjustable tine attachmentOptions and Specifications. Hand tractors are available with either petrol or diesel engines ranging in size for 5 to 18 hp, with more powerful units offering greater capacity. The machines allow for a range of operations including seasonal ploughing, harrowing, and forming beds, and later in the season for weeding between crop rows and in orchards. In addition, hand tractors may be attached to trailers to transport goods and people. Clearly, opportunity exists to engage in the trade, sales, and servicing of hand tractors as their popularity and accessibility grows. Companies specializing in the wholesale importation of these machines and their attachments are needed to meet growing demand, and the competition between such companies will bring prices down. Opportunity exists to design attachments that better meet small-scale farming needs, and for decent employment through the assembly and maintenance of these machines. Ideally, agrodealers will begin to market these machines within their shops as well. Finally, land preparation using hand tractors will be offered as contract services by local operators to reduce drudgery among a growing number of small-scale farmers. A hand hoe consisting of an iron blade and short wooden handle is the most common weeding tool, and it is closely associated with the drudgery and continued poverty of small-scale farming.A power weeder is an alternative approach to weed management through secondary tillage that greatly relieves drudgery associated with hand weeding. Two basic types of power weeders are available; ones that are worn on the operators back where weeds are cut and buried to shallow depths through arm movement (power backpack weeders) and others that resemble small walking cultivators that pass between crop rows chopping weeds and burying them to adjustable depths (mini-cultivators). The machines require skillful use and maintenance to ensure that crop plants are not injured in the process of weed removal and that the machinery remains in good working order.Advantages. The advantage of mechanized weeding is that the control of unwanted plants is achieved without heavy field labor. This control is best achieved within a wider strategy of integrated weed management, particularly through the judicious use of pre-emergent and selective herbicides. Using mechanized weeders, it is possible for a single operator to greatly reduce weeds from 0.5 to 1.0 ha per day, particularly for field crops with widely spaced rows and in orchards with open space between perennial plants. An advantage of mechanical weeding is that operators need not wear heavy protective gear other than boots and in some cases dust masks. One disadvantage to mechanized tillage is when rocks or soil clods clog the rotating blades, causing the machine to be temporarily shut down for the blockage to be cleared. Another difficulty is the cost and the inability of poorer households to afford them.Options and Specifications. As stated above there are two basic types of power weeders; backpack weeders and mini-cultivators. A typical backpack weeder consists of a petrol engine and small fuel tank mounted onto a padded backpack frame connected to a flexible power hose leading to a handle ending in different replicable power heads bearing either rotating or circular blades. The handle is controlled with grips that also allow access to an on/off switch.The small petrol-powered engine (e.g., 35 to 50 cc) is air cooled and intended to operate at a continuous speed without overheating. These machines weigh between 6 and 9 kg and allow operators to weed up to 1 ha in a day using only 2 liters of fuel. Because the rotary blade sits upon and digs into the soil there is little arm fatigue during its use. Use of this machine is 10fold more labor efficient compared to hand weeding with a hoe. An advantage to this machine is the wide range of different heads that can be affixed to it that may be used for cutting brush, levelling tilled fields, harvesting field crops, and even powering small water pumps. These units are available at a cost of US $240 to $380 each, depending on the size of the engine and the number of different heads included with the purchase.Mini-cultivators are fundamentally different machines that serve a similar purpose in terms of weeding, but lack the wider utility of the other backpack attachment heads. A small petrol or diesel engine (e.g., 2 to 3 hp) is mounted onto a wheeled frame that leads to handlebars at one end and rotating tines at the other. In general, these blades are 17 to 25 cm in width, incorporate materials to a depth of only 2 or 3 cm and weigh about 20 kg. While most of these machines are intended for single, well-spaced rows, modified versions allow for 2 or three closely spaced rows of vegetable to be weeded as well. Note that smaller hand tractors with adjustable tines may be used for this purpose but generally clean wider paths and dig to deeper depths. Either wheels or the rotating tines may serve as traction to pull the cultivator forward along a course determined by the operator's use of the handlebars. Some models include tail \"anchors\" that affect the depth of soil disturbance. Note that the tines must be set between rows with sufficient space away from plant stems to avoid any damage to plants.In general, these machines are available at a cost of US $200 to $300 each. to swing the nozzle in a steady, sweeping motion to minimize this effect. The greatest advantage is that the application of sprays is directed by a human who is in a better position to exercise caution when applying potentially dangerous materials to agricultural crops and cultivated lands.Options and Specifications. As stated earlier, there are two basic types of power sprayers: backpack and trolley. A backpack sprayer typically has a 16 to 25 m tank containing spraying solution, a 33 to 50 cc petrol engine and 0.5-to-1.0-liter fuel tank, and a short hose ending with adjustable wands or spray guns. In general, this machine weighs 10 kg and can deliver sprays about 5-times more rapidly than pump action backpack sprayers, covering about 0.5 ha per hour. A trolley sprayer is mounted on a wheeled frame consisting of the 50-to-200liter tank, 3 to 6 hp pump, a fuel tank and up to 50 m or so hose, usually mounted on a reel. The trolley remains stationary while the operator deploys the hose, delivering up to 25 liters of stray per minute. One disadvantage is that operator movements with the trolley hose are more limited, and care must be made not to injure plants as the hose is deployed and moved.An advantage of trolleys is that they may be fabricated using local materials and customized to specific needs. Both types of strayers require calibration to be used properly.There is a three-step method to calibrate these sprayers.Step 1 measures and marks off an area equal to 500 square meters (such as 20 x 25 m).Step 2 adds a measured amount of water to the tank, sprays the area and then measures the amount of water remaining in the tank. The difference between the amount in the tank before and after spraying is the amount used per 500 square feet (= 0.05 ha).Step 3 compares the measured application rate with the recommendation on the pesticide label. If the difference between the recommended rate and the measured rate is greater than 5% of the recommended rate, adjustments are made to reduce the application error. Note that operators must wear proper safety equipment including gloves, boots, eye protection, water resistant jackets and hoods and masks.Availability and Commercial Opportunity. Judicious use of agrochemicals is fundamental to Africa's agricultural transformation and use of power sprayers provides a means to this end.Their use allows farmers the ability to intelligently alter application practices as conditions warrant. Compared to pump action sprayers, this equipment is not yet widely available, and this may be corrected by agricultural product suppliers stocking them side-by-side. In addition, availability of power strayers provides greater opportunity for contract service provision to farmers as a rural enterprise that in turn leads to more effective control of pests.Recent biological invasions by Fall Armyworm and the Yellow Desert Locust were met by these actors but there is scope for greater linkage to farmers. It is important that wherever these sprayers are marketed, so too is necessary personal protective gear. Agricultural extension advisers must be aware of this agrodealer linkage and ensure that precautionary messages on pesticide safety are available through it. That trolley type sprayers can be fabricated locally is also an important consideration as it may contribute to growing agro-industry, and the comparative advantages of backpack versus trolley approaches must be considered.Technology 4. Earth Augers as a Labor-Saving ToolBackground. An earth auger, also called a post-hole digger, is a machine used for drilling holes into the ground. It consists of an engine powering a vertical shaft that rotates screw blades that rotate to displace soil, resulting in a cylindrical hole. It is used to efficiently dig uniform fence post holes and planting holes.Holes results from a rotating helical screw blade winding around lower part of the shaft. The lower face of the screw blade progressively displaces soil from the bottom of the hole, and the remaining screw blade serves as a conveyor to lift the loose soil upward to the soil surface. When the hole achieves a desired depth and the tool is withdrawn, the blade removes any remaining soil. The rod often ends in a sharp point that keeps the auger along a straight path. The screws are available in various sized that control the width of the hole, and there are extension bits that allow for steeper penetration. A skilled operator can prepare a 40 cm deep hole 25 to 30 cm wide in less than a minute.Advantages. The main advantage of an earth auger compared to a manual post hole digger or shovel is savings in terms of the time and effort required, and the uniformity of the resulting holes. The precise width of the holes is determined by the diameter of the screw blade and the depth may be controlled using extensions. Manually digging a series of holes results in fatigue that leads to less uniform holes with irregular shape, including sloped walls. More precise holes allow for more reliable preparation or backfill materials, whether they be cement and gravel for fence posts or enticed soil for planting holes.A representative, commercially available earth auger includes a 64 cc, single cylinder, two-stroke petrol engine with a 1.3-liter fuel tank containing a fuel to oil ratio of 25:1. It sells for about US $230. The engine is mounted onto a metal frame with handles that allow control by the operator and ready access to its throttle. that Its drill bit length is 0.8 m but with available extension bit lengths of 0.35, 0.5 and 0.8 m, allowing for drilling depths of 1.6 m. Different drill diameters may be attached available in diameters of 6, 8, 10, 15, 20, 25 and 30 cm. A more powerful auger with a 2 hp, four-stroke engine costs US $450, including an assortment of screw blades (15, 20 and 25 cm bits). Individual replacement blades cost between US $30 and $50 depending upon their width, and extension bits are US $25 to $30 depending upon their length.Availability and Commercial Opportunity. Use of land augers is far more common in the construction industry than among farmers, particularly smaller-scale ones. A quick survey of earth auger suppliers discovered numerous suppliers in Kenya, locally marketing several different single operator petrol powered models as both agricultural and construction equipment. In addition, other options included two-operator models with wider and deeper blades and much larger augers attached to external power sources. Local suppliers were also readily identified in Nigeria, Uganda, and Zambia, although queries for other countries suggested that local suppliers act as importers on demand. Most of these imported models were linked to manufacturers in China. A quick search of suppliers in China discovered over 15 manufactures, revealed over 25 single-operator, petrol-engine models, some as powerful as 80 cc, others available for as little as US $50 each with no minimum order, and yet others supported by a wheeled frame that guides the auger as it drills. Clearly, many options and price ranges are available to those seeking to import and distribute this equipment. Also, a 4-stroke, 196 cc, 6.5 hp two-operator model with a bit size 70 cm in diameter is sold for less than US $200 each.An important emerging use of earth augers equipped with wider screw bits is the rapid scaping of soil pits as a water harvesting practice. \"Zaï\" pits are a dryland farming approach developed in the Sahel. These pits are formed by digging shallow basins of 20 to 30 cm diameter and 10 to 15 cm deep into croplands, allowing the pit to collect water, wind-driven soil particles and plant debris. Early evidence suggests that forming these pits using an earth auger rather than hand tools is at least five time faster. On sloped lands, the soil excavated from the pit is placed downslope to better permit water catchment, a step that requires use of hand tools. Note that this same technique is also used to rehabilitate crusted and degraded lands. Greater availability of earth augers used for this purpose will likely increase this practice in dryland agriculture and allow greater expression of its climate-smart features.Background. Drip irrigation is a system that slowly delivers water onto the roots of plants in a way that strategically places moisture and minimizes evaporation. It is the most efficient method of irrigating with over 90% of its water utilized by crops. It is relatively easy to install and reduces disease problems associated with wet leaves. Drip irrigation systems distribute water through a network of valves, pipes, tubing, and emitters that operate at a relatively low water pressure. Components used in drip irrigation include a pump or pressurized water source, a water filter and particle separator, a backflow check valve and simple pressure regulator, a distribution mainline, control valves, smaller diameter laterals (or sub-mains), and emitters that deliver water at slow rates. While complicated in design and somewhat expensive to install, the savings in water and yield improvement is substantial.Advantages and Disadvantages. The advantages of drip irrigation include high water use efficiency with minimal leaching of fertilizers, ability to irrigate unleveled and irregularly shaped fields, and greatly reduced weed growth and soil erosion. Drip irrigation delivers its water to the crop root zone rather than the field at large. Soluble fertilizers are readily injected through water delivery. Drip irrigation does not wet foliage, reducing the risk of disease. In addition, drip systems operate at lower pressure than others, reducing their energy cost. The disadvantages of drip irrigation include its high initial cost, sensitivity of tubing to UV light, clogging of the emitters by sediment, and additional \"cleanup\" costs after harvest as the tubing must be rewound and stored for use. In sandy soils, drip systems may be unable to wet the soil surface for uniform germination. Most drip systems result in little or no leaching, causing salts to accumulate. Finally, pipes may suffer from rodent or insect damage, requiring replacement and increasing expenses. Because of the way the water is applied in a drip system, traditional surface applications of fertilizer are sometimes ineffective, so drip systems often mix liquid fertilizer with the irrigation water, a practice referred to as \"fertigation\" and resulting in substantial fertilizer savings.Options and Specifications. A typical drip irrigation system consists of a pump unit; a control head; mainlines and sub-mainlines; lateral lines; and emitters or drippers. The system may include additional features such as reservoir tanks and fertigation injectors. The pump unit takes water from the source and provides the correct pressure for delivery into the pipe system. The control head consists of valves to control water pressure and filters to clean the water. Mainlines, sub-mainlines and lateral lines supply water from the control head to the fields and are usually made of PVC or polyethylene hose. These water delivery lines are often buried. There are two types of drip irrigation: sub-surface drip irrigation where water is applied below the soil surface, or the much more common surface drip irrigation where water is applied directly to the soil surface. Sub-surface irrigation employs narrow plastic tubes buried in the soil at a depth between 20 and 50 cm. The tubes are either porous throughout or are fitted with regularly spaced emitters. Major difficulties with this approach are the narrow orifices of the emitters that may become clogged by roots, soil particles and precipitating salts; and that tillage practices become complicated. As this approach is not very common it is not considered further.Surface drip irrigation is much more common and uses a variety of drip emitter devices. Lateral lines fitted with emitters are placed on the soil surface along crop rows. Pressure is low so the water emerges as drops rather than as spray. Emitters control the discharge of water from the lateral lines to the plants, with one line used to irrigate one or two rows of crops, depending upon their spacing. Emitters are placed off the soil surface to prevent clogging and are calibrated to release between 2 to 16 liters per hour. The ends of the lateral lines are periodically flushed to discharge particulates. Drip irrigation emitters are \"pressure compensating\", meaning that they can discharge water at a very uniform rate under varying water conditions. This trait allows for more constant flow under fluctuating input pressure and different slopes. Some drip irrigation emitters are built with a self-flushing mechanism reducing the risk of clogging, others are flushed by opening the end of each line.Availability and Commercial Opportunity. One acre (0.4 ha) of land may be placed into surface drip irrigation for about US $1120 with costs as follows: drip lines (US $770), pump and filter ($166), poly main and sub-mains ($100), and valves and fittings ($88). Drip irrigation is closely associated with greenhouse horticulture and higher value crops. A quick internet search of drip irrigation suppliers in Kenya revealed over 20 suppliers of drip irrigation materials, including many suppliers outside of Nairobi. Some suppliers provide complete \"kits\" for 1/8 and 1/4 acre. In many cases, suppliers will assist in the design of these systems as they sell the materials required for it. Another search revealed 14 suppliers in Lagos, Nigeria but fewer suppliers away from the city. Opportunity exists to develop agribusinesses around the design of drip irrigation systems and the sales and installation of its materials, but several African countries still do not have ready access to this technology.A small greenhouse equipped with drip irrigationBackground. A rain gun is an irrigation device that supports high water flows and an extended radius of \"water throw\" through its sprinkler. They allow relatively few stations to cover large areas of field, and at the same time are portable, allowing a gun and its stand to be moved to different field locations according to needs and schedules. These guns require high water pressure and flow and can project water for up to 60 m in distance that covers a circular area of 1.1 ha. This coverage allows these systems to be installed quickly and cost-effectively assuming the conditions for their reliable operation are met. At the same time, adjustable jets allow control of the throw, droplet size and impact to suit the irrigation needs of more delicate crops.Advantages and Disadvantages. Rain guns offer major advantages to farmers seeking to place substantial areas of land into irrigation is a simple way but also require that precise conditions in terms of water quality, flow and pressure be met. Each water gun is mounted upon a stand that raises it off the ground so that its throw does not disturb adjacent plants. The base of the stand is connected to a water source, in most cases a flexible hose like that used by firefighters. This allows the rain gun and stand to be moved to different stations so that only one gun covers large areas of field (e.g., 4 to 6 ha) depending upon water requirements and irrigation schedule. The coverage is adjustable in terms of distance, droplet size and completeness of circular angle, with 360 o projection most common. Claims exist that the force of rain gun water is sufficient to disrupt the activities of some pests. At the same time there are disadvantages, even beyond the exacting requirement for water. The rain gun operates from a central pivot position that is not suited to smaller or irregularly shaped fields. Because it operates at such high pressure, the rain gun must be handled with caution and its parts must be well maintained and regularly inspected to ensure against wear. Each rain gun requires substantial investment and is usually serviced by a single high pressurehigh volume water pump that must also be purchased and maintained. Strong winds adversely affect water coverage. Rain guns allow farmers that have relied upon rainfed production of field crops in the past to adopt irrigation as a practice, reducing risks of climate change Options and Specifications. Rain gun technology is designed for a variety of uses and applications where relatively high flows and extended radius of the water throw are required.These sprinklers operate at pressures of 2.5 to 7.5 kg/cm 2 and flows of 5 to 30 liters per second. Below these thresholds, the gun does not function in a uniform and reliable manner.The nozzle diameter is in many cases interchangeable, 10 -30 mm, resulting in wetting radius of 25 to 60 meters. At highest flow rates, one position can deliver sufficient water for crop irrigation in as little as 2 or 3 hours, allowing for repositioning several times a day.Availability and Commercial Opportunity. Rain gun and their accompanying materials are not widely available in many African countries, but it is important that they become so. A quick search in Kenya discovered over 10 suppliers in Kenya, including those away from Nairobi, as well as importers from China and India. They were readily available in Uganda as well, although some only throw water 30 m. Similar findings were obtained for Nigeria although there was not a clear distinction between rain guns and more conventional sprinkler apparatus. The advertisements in Zambia were mostly posted by importers rather than local suppliers but electronic discussion on rain gun technology is ongoing. Many other countries lack suppliers, and this provides commercial opportunity.A complete rain gun irrigation system was recently purchased in Nairobi for US $669 after comparative shopping.The components and costs were rain gun head (US $140), tripod stand ($90), 90 m hose ($110), 2\" high pressure 7.5 HP water pump ($290) and 6 m suction hose ($39). These systems are affordable to farmers conducting small-scale commercial enterprise and must become more available across Africa!The stand of a rain gun; note that water enters from the base through a threaded fitting and is delivered to the rain gun mounted at its topBackground. Threshers are power equipment that separate crop residues from seed and grain in a time-efficient manner. These machines are powered by small petrol engines and consist of a feed chute that leads to a threshing chamber where crop residues are separated from seeds in a rotating drum, and then a blower removes lighter residues. Operators put in dried harvest materials through a feed chute, pushing materials into an internal spinning drum where seeds are physically separated from crop residues and then fall through a screen. Whole shoots may be passed through the machine rather than pods and heads alone. Remaining crop residues are then expelled through an exit chute. These chopped materials have further use as organic resources. The seed is passed across a blower that removes finer materials (e.g., dust) that winnows (cleans) the seed, passing through a collection chute that allows the seed to be bagged. Different types of crops may be processed based upon the screen mesh. Threshers are available to process crops such as maize, rice, common bean, wheat, sorghum, millet, sunflower, and pigeon pea.Advantages. Typically, women are assigned the task of manually threshing crops by hitting piled harvest with sticks until the grain falls loose, a task that requires about one hour of work to recover 25 kg of seed. Small-scale mechanical threshers can process seeds and grain many times more rapidly than traditional threshing and winnowing operations. Mechanized threshing is very labor efficient, allowing for the processing of between 150 to 500 kg of saleable product per hour, depending upon the crop and machine. The smaller the seed, the more rapid the processing time. Not only is processing more rapid but also more thorough because the in-built blower cleans grain more completely than traditional winnowing. Mechanized threshers cause less breakage to grain and seed than manual beating which gives farmers a quality and market price advantage.Mechanized threshers separate and clean grain and seed in one single operation, making produce ready for sales on local markets or trading without need of further processing.Power thresher processing sorghum grain Options and Specifications. Different types of power threshers are available which are distinguished according to the type of crops they process (e.g., single crop or multiple crops), according to their mode of operation (e.g., batch drum or axial flow), and the type of threshing cylinder (e.g., syndicator, beater, spike tooth and rasp bar). The smallest threshers weigh as little as 100 kg and may be mounted on motorcycles for on-farm use. Portable threshers are positioned next to piles of harvest on a level surface. Larger machines may be transported by small trucks and established within communities for collective use by farmer groups. Threshers have a modular design, and their basic components include a feeding chute, threshing cylinder, aspirator blower, chaff outlet, straw outlet, hopper, and cam for oscillating sieves, oscillating sieves, transport wheel, frame, and main pulley. Small engines (5 to 8 HP) that consume only 1 to 2 liters of fuel per hour typically operate these threshers. These threshers are often mounted on wheels and have handles that permit their movement. A tarp is set below the collection shoot to keep the seeds clean and to facilitate bagging. Residues must be periodically raked away as they are ejected from the exit chute. It is extremely important that operators of these machines must be trained in the maintenance, minor repair, and safe use of this equipment and that they be operated without distraction (see section \"Safety First!\").Availability and Commercial Opportunity. Some threshers are produced in Africa, and a large number are available for import. A quick internet search gives 110 thresher models offered by suppliers from India and 52 from China, although some were intended for use on only one crop or for small grains only. There is scope for commercial provision of threshing services that make better quality grain available to households sooner after harvest. This post-harvest service may be provided by independent business interests or as a means of assuring grain quality to produce buyers. Marketing threshers is a different matter and involves either distributing fabricated equipment or importing it. Large discounts are available to those importing equipment in larger quantities. Multi Background. Residues and stover from crops offer an important source of livestock feed but owing to their bulk their preparation using manual labor is extremely time consuming. When animals are herded over croplands after harvest, only 20-30% of stover is eaten since they prefer the sweeter parts that are easier to digest. Access to quality feed is the most important factor in successful livestock rearing. Many farmers feed whole stover to their animals which slows down their digestion and causes sub-optimal growth. Motorized residue processing is ideal for mixed crop-livestock farming, particularly where underutilized crop residues are plentiful, and costs of animal feed are prohibitively high. Small-scale motorized cutters facilitate collection of stover from the field, allowing residue recovery from several hectares in a day. Use of motorized choppers and crushers make it possible to provide suitable feed and mulch for soil cover while saving time and effort. Depending on the chopper model, throughput capacities range from 1 to 1.5 ton of stover per hour.Advantages. It takes a lot of time from farmers to manually collect crop residues from the field and chop these into small pieces by hand so it can be consumed by cattle. The manual process limits the amount of stem residues that farmers utilize for livestock. Motorized cutters and choppers address this constraint and improve organic resource management within the farm. The machines are self-powered, easy to operate, low-cost, easily transported between fields, and allow large amounts of crop residues to be processed from fields by only two workers. These machines are suitable for a wide range of fresh and dried materials available throughout the year Mechanized residue processing benefits storage and preservation of feed products by making it possible to compact the material in bags that can tightly packed instead of loosely piling whole stover into a shed. Packing enhances flavor andUse of motorized stover cutter (left) and mobile chopper (right)nutritive value as well. Increased availability of chopped and shredded residues from crops is fundamental to local production of well-balanced feed rations. Chopped and crushed stover from cereal and legume crops is also suited to produce silage. Through mechanized residue processing, farmers can earn additional income, rear larger numbers of animals, increase milk and meat yield, and avoid feed shortages during dry seasons or prolonged drought. The technology serves both animal and crop production since residues fed to livestock produce manure which in turn improves soil fertility when returned to the field.Options and Specifications. Motorized chopper machines can be used for either fresh and dry plant materials from a wide range of cereal crops like maize, sorghum, and millet, as well as legumes such as soybean, groundnut, and cowpea. Chopping works best for green stover before fibers harden, while crushing is mostly done after residues have dried. Chopper machines have four main parts; a pair of horizontal rollers that moves stover forward, a hexagonal shear cutter with knifes, a hammer for crushing the chopped stover, and a 7 to 13 horsepower engine running on petrol or diesel. Choppers and hammers work at the same rate as the roller to ensure uniform sized feed material. Material is further ground through the beating action of the hammers until it passes through holes in an adjustable screen. Crushed material is pushed forward by the motion inside the chamber. Residue processing machines are easily transported between fields and farms using a donkey cart of motorbike. Most residue choppers are fitted with wheels.Motorized cutters and mobile choppers are commercially available in many mixed crop-livestock farming communities across Sub-Saharan Africa. Demand for the technology is rapidly growing thanks to its many advantages and dissemination by national agricultural development agencies. Motorized cutters that can handle all types of cereals cost about US $1,000 to $1,500 on international markets. Local sales prices for new stover choppers with in-built engine ranges from $1,250 to $1,700 depending on the size, the manufacturer, the country of origin. Imported models are usually more expensive than locally fabricated ones. This small-scale equipment may be offered as a package to individual farmers, their associations, other service providers or feed producers. Processing stover from cereal and legume residues offers an attractive business opportunity since added value is created and market demand exists. Return on investment depends on the cost of whole stover, labor, fuel and maintenance, cost conditions that vary between locations and times of year. Machines provided to farmer associations led the production and sales of more than 100 tons of stover worth US $22,000 in less than six months. On animal feed markets in many African drylands, one ton of crushed stover sells for US $330 to $500 depending on the quality and time of year. Starting a forage chopping business may indeed be a timely investment!It is extremely important that the smallscale machines featured in this catalogue be handled in a safe and responsible manner, and in accordance with manufacturer's instructions. Some guidance in these safe operations follows.Hand tractors. The danger from hand tractors (Technology 1) is damaging feet and lower legs by the rotating tines and operators must remember to wear protective footwear and keep feet well back from power train. Another danger exists when roots and rocks bind the tines, and operators must turn off the power before disengaging them. Operating hand tractors on steep slopes is inherently dangerous. Even when turned off, the times may recoil so tools, not hands, should be used when cleaning these machines after each use. Children should not operate hand tractors despite their enthusiasm to do so! Mechanized weeding operations. Mechanized weeders pose the same dangers to users as hand tractors, particularly the mini-cultivators (see Technology 2). Because they are lighter in weight, the tendency is to swing them around quickly to avoid contact with crops. Again, tines may become bound by roots, rocks and clods, and operators must turn off the equipment and rely upon tools before disengaging them because the tines may recoil once freed. Particular care must be taken when using the circular rotating blades of backpack weeders to trim woody vegetation because they tend to be swung in a sidewise motion away from the ground and they may recoil causing loss of control of this devise, even posing a danger to others.Power sprayers. Operators must always be aware that power sprayers (Technology 3) can discharge large amounts of harmful substances and wear protective gear. This gear includes respirators, eye shields, gloves, water resistant clothing and boots. Spraying and wash up must be performed in an environmentally responsible manner and according to product specifications. Many locations have specific regulations on the safe disposal of pesticides and their wash water and special care must be taken not to contaminate waters and farm animals.Land augers. The greatest danger from augers (Technology 4) is injuring the operator's feet or legs with the rotating screw. When encountering rock or roots, the device may recoil out of control and operators must be solidly positioned against this. If the bit jams, one must turn Despite the excitement, operators must have sufficient strength to handle power equipment Operators of sprayers must always wear protective gear (right, never left) off the auger before dislodging it and always keep hands away from rotating screws. Operators must always wear protective boots and resist the temptation of rushing from one hole to the next with the machine engaged.Rain guns. The safety precautions taken during installation, use and dismantling of a rain gun (Technology 6) include ensuring that the gun is securely fixed to its stand, avoiding the fast reverse rotation of the gun as it operates, and ensuring that \"quick couplings do not slip and discharge uncontrolled. Adjustments to the gun's diffuser screw should not be made as it operates and once in operation, one should remain at least one meter away.Power threshers greatly reduce labor requirements, but their operations are inherently dangerous unless the machinery is operated as intended (Technology 7).Threshers require that crops be fed manually through a feeding chute into a spike-tooth cylinder and chaff-cutter that can seriously injure hands. Human factors such as inattentiveness, overwork, wearing of loose clothing, failure to remove wristwatches and bracelets, and use of intoxicants greatly increase this risk. Threshing accidents can be minimized through the following measures: 1) Threshers must be fitted with a safe feeding chute at least 90 cm in length with half of that covered, 2) Only skilled and trained workers should operate a thresher who avoid talking and unnecessary distractions while performing their duties, 3) Extra care must be taken when feeding crops lacking stalks into the thresher as this requires closer proximity of hands to moving parts of the machine, 4) Ensure proper lighting if the machine is to be operated at night and the area surroundings the thresher must be kept free of obstructions, and 5) Do not smoke or light a fire near the threshing yard as the dust and residues from the thresher are extremely flammable. The thresher workstation should include a first aid kit.Forage choppers. Safety must be practiced when using forage choppers (Technology 8) because of their several moving parts resulting in cutting action. Operators are expected to push fresh or dried plant materials into a chute so that it is cut into various sizes, and then collect and remove chopped material as it accumulates. Equipment must be carefully inspected, and users must understand the importance of protective shields as they operate the machinery. Care must be taken not to feed the chopper woody material or rocks that can damage the blades. Forage crops are often grown on rough and steep land, and the choppers are portable, but operators must not set the chopper on land that is not entirely stable. Otherwise, the same safety precautions apply to both threshers and forage choppers. This section serves only to highlight the extreme importance of safety in the operations of small-scale farming equipment as these machines are intended to reduce human drudgery and not cause injury. Most accidents result when operators become rushed or overconfident.The aversion to careers in agriculture among young people results from the perception of necessary drudgery and poor returns to effort. Wider reliance upon small-scale farming equipment serves to counteract this misconception. One important mechanism to promote this equipment is through the activities of youth groups, allowing for skills development in the use, maintenance, and safe handling of these machines. This route is particularly relevant when youth undertake agribusiness incubation leading to their development of innovative agribusinesses and modernization of their home farms, affirming that they are no longer bound to practice agriculture in ways overly reliant upon manual operations. In this way, mindset change results from exposure and hands-on learning, allowing youth to embrace agriculture as a mechanized and profitable pursuit, and to find commercial opportunity in advancing mechanization as a growing trend across the small-scale farming sector. Agricultural mechanization and modernization equipment allow young producers to optimize their time and reduce production costs. Furthermore, larger farming communities demand for mechanization services from youth create opportunity for further income generating option. Several examples of this trend follow.In Zambia, the use of a hand-held tractor for land preparation and furrow making is less costly and more labor efficient, increasing crop yield and profit for young rural entrepreneurs. A variety of attachments is available to not only rotovate the soil but to also establish raised beds, irrigation furrows and erosion control structures. Similar youth enterprises in Uganda and Kenya also provide mechanized weeding services to farmers.Youth are actively engaged in the control of Fall Armyworm and the Yellow Desert Locust, two insect invasions with dire consequences to African farmers. Reliance upon a power sprayer and alliance with agrodealers networks increased the efficiency of this service provision. At the same time, youth were well positioned to access control information via electronic media and to comply with health and safety requirements. Farmers discovering widespread invasion of maize fields were particularly motivated to see contractors provide rapid services that saved their crop. Reliance upon power sprayers allowed operators to treat fields five times more rapidly than others using pump action backpack sprayers, and to offer better coverage of leave undersides and inside whorls at the same time. Youth-led Rapid Response units operating from agrodealer shops developed toolkits consisting of a customized cargo tuk-tuk, power sprayers, safety equipment, commercially recommended pesticides, farmer information materials and communication tools. One group in West Kenya serviced 227 clients, treating an average of 0.20 ha per client at a cost of US $5.68 each, equivalent to only US $28 per ha. Subscription to this service resulted in a return to investment of 4.1:1 in terms of rescued maize. Similarly, youth offering spraying services to farmers earned approximately US $2000 a month in Uganda.A team of youth in Uganda founded a business called \"Mr.Clean\" that weeds and plows land for a fee. Over time, their services included contracts with the Uganda National Roads Authority to control unwanted vegetation along roadsides. Now they offer assistance to other youth-led startups unable to afford their own machines.Youth have capacity to operate at the cutting edge of technology. Some developed expertise in the installation of pond aerators enhancing the levels of oxygen in fishponds and greatly improving water quality and fish health. Others developed expertise in the operations of drones used for small-scale aerial spraying of crops. Youth in Kenya adapted land augers to quickly prepare water-harvesting features for dryland agriculture, mechanizing the establishment of a climate-smart cropping practice and reducing its labor requirement four-fold.These opportunities have an institutional dimension as well. The ENABLE-TAAT Compact works in partnership with the Government of Benin to introduce small-scale mechanized farming to the country's youth. The greatest opportunity, however, resides with the private sector. Once skills in the management of small-scale farming equipment is obtained, it is possible to grow an importation and retail business around this expertise. The price difference between these different equipment sold in quantity and the local sales prices in Africa are quite large, allowing for reasonable profits to be made from modest turnover of stock. These machines once sold serve as the focus for additional youth-led businesses that offer contract services to farmers, reducing the drudgery associated with agriculture in an incrementally improved manner.Youth preparing a sprayer drone for flightTAAT offers its services toward the advancement of modernized agriculture. It brokers a wide range of needed technologies and bundles them through a process of co-design into winning solutions. It recognizes that modernized agriculture must serve as the main engine for economic growth in Africa and operates accordingly. Change is intended to achieve not only food and nutritional security but also to meet obligations under climate agreements allowing collaborative efforts to better combine global, national, and community-level interests. TAAT operates from this unique perspective to mobilize innovative solutions through better partnering that includes honest technology brokerage and effective, scalable skills development through five key mechanisms. Unique understanding. Expertise is offered in the areas of site characterization and problem identification. Innovative solutions. Leadership is provided in technology brokerage and solution bundling based upon a dynamic portfolio of candidate technologies. Better partnering. Assistance is offered in the better co-design and management of projects prompting agricultural transformation. Honest brokerage. A robust capacity for impact assessment and constructive learning is achieved through standardized monitoring and evaluation.This catalogue describes the role of small-scale farm machines within Africa's agricultural transformation and several of that equipment that are most important to that process. Small-scale farming is widely associated with lives of poverty resulting from subsistence agriculture and drudgerous manual labor. This perception must be changed if commercialized agriculture is to drive rural economic development, particularly among talented youth that will provide farming's future generations. Too often, these youth are seeking ways to escapeUser queries directed to the ProPAS website offering information on agricultural technologies agriculture as a career path rather than committing their lives to exciting new enterprise opportunities. Mechanization is key to this commitment, both in terms of greatly reducing manual labor associated with core farm tasks, but also in designing livelihoods around the innovative services that support mechanization. The growing importance placed upon mechanization by the agricultural community is reinforced by recent findings of the ProPAS internet site where more queries were directed toward mechanization technologies than any other category.Contract services offering use of these equipment are particularly important. Reliance upon hand tillage often results in portions of fields remaining idle because there was insufficient time and labor to prepare them for planting. Cultivated farmer fields often become weedy for the same reason. Insect invasions go unchecked because their swarms overwhelm available control options. Droughts destroy crops in places where irrigation water is only slightly out of reach. The market value of grains is reduced because manual threshing results in excessive damage to processed harvests. All of these constrains are readily met with technologies presented in this catalogue!The advantages of power tillers, weeders and sprayers are obvious to anyone who has undertaken their laborious manual alternative, but challenges must be met to make this machinery available and to keep them functioning. Equipment suppliers must extend their sales further into rural areas, and agrodealers must be willing to invest in and market this equipment. Farmers must join together to purchase and share these machines. Extension agents must promote mechanization and mechanics must develop the skill sets needed to maintain and repair the equipment or else it risks falling into disuse. Development specialists must recognize the extreme importance of small-scale mechanization to Africa's agricultural future and accommodate this need within the formulation of rural development projects through the design of public-private partnerships. It seems the smaller the equipment, the more relevant it becomes to poorer farmers, and incentives must be offered to these labor-saving machines within their reach, and to create jobs for their families as service providers of these equipment into the future.The development objective of TAAT is to rapidly expand access of smallholder farmers to high yielding agricultural technologies that improve their food production, assure food security, and raise rural incomes. This goal is achieved by delivering regional public goods for rapidly scaling up agricultural technologies across similar agro-ecological zones. This result is achieved through three principal mechanisms; 1) creating an enabling environment for technology adoption by farmers, 2) facilitating effective delivery of these technologies to farmers through a structured Regional Technology Delivery Infrastructure and 3) raising agricultural production and productivity through strategic interventions that include improved crop varieties and animal breeds, accompanying good management practices and vigorous farmer outreach campaigns at the Regional Member Country level. The important roles of sound policies, empowering women and youth, strengthening extension systems and engaging with the private sector is implicit within this strategy. The Clearinghouse is the body within TAAT that decides which technologies should be disseminated. Moreover, it is tasked with the responsibility to guide the deployment of proven agricultural technologies to scale in a commercially sustainable fashion through the establishment of partnerships that provide access to expertise required to design, implement, and monitor the progress of technology dissemination campaigns. In this way, the Clearinghouse is essentially an agricultural transformation incubation platform, aimed at facilitating partnerships and strengthening national agricultural development programs to reach millions of farmers with appropriate agricultural technologies.Back cover photographic credit: Correct (left) and unsafe (right) personal protection while spraying pesticides.","tokenCount":"8410"} \ No newline at end of file diff --git a/data/part_6/0484344599.json b/data/part_6/0484344599.json new file mode 100644 index 0000000000000000000000000000000000000000..9de1062244fc909927ffd4310b0aa3adca96e713 --- /dev/null +++ b/data/part_6/0484344599.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"e0a51cbeaf48a8ebfe40e9db85ef6933","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/f36e9e48-fc38-4ab4-b880-f8dff77b4513/retrieve","id":"1102606769"},"keywords":[],"sieverID":"ffe2c34d-06b9-4104-9011-0ca11ef58758","pagecount":"2","content":"The ILRI forage germplasm information system was developed to share information about the germplasm held by ILRI as a global public good under the International Treaty on Plant Genetic Resources for Food and Agriculture.The web site includes information on the collection sites of material held in the ILRI genebank, as well as summary characterization data and an image collection of some of these materials. It also has factsheets on the management of some common forage species.GENESYS is a rich source of information on plant genetic resources diversity of seeds conserved in genebanks worldwide. GENESYS is a gateway from which germplasm accessions from genebanks around the world can be easily found and ordered. GENESYS is the result of collaboration between Bioversity International (www.bioversityinternational.org/) on behalf of the CGIAR System-wide Genetic Resources Programme (SGRP), the Global Crop Diversity Trust (www.croptrust.org/) and the secretariat of the international Treaty on the Plant Genetic Resources for Food and Agriculture (www.planttreaty.org/). GENESYS helps to secure the long-term conservation of plant genetic resources.The Forage Register is another initiative of the SGRP. It contains passport data for forage accessions conserved in a number of major collections of plant genetic resources. ","tokenCount":"192"} \ No newline at end of file diff --git a/data/part_6/0487415979.json b/data/part_6/0487415979.json new file mode 100644 index 0000000000000000000000000000000000000000..a124a82c852b59adf703e3018162d14e34e054f4 --- /dev/null +++ b/data/part_6/0487415979.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"0729a159608c58489d1fa07de7ba0308","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/7f950fff-a47f-4e5d-aa14-2b84603f438b/retrieve","id":"1234197505"},"keywords":[],"sieverID":"bcd202e5-45ed-4f1d-959a-58c2897a5979","pagecount":"23","content":"Lack of rapidly available, site-and system-specific knowledge about such solutions and their potential environmental impacts What is CLEANED? The CLEANED tool lets users explore multiple impacts of developing livestock value chains in explicit ways.These indicators fully align with the environmental dimensions of concern, as prioritised by the global experts.The Architecture • Rapid results: flagging potential environmental issues associated with planned livestock development strategies→So that environmental results can be considered when designing livestock development/investment programs and policies →Promotion of sustainable livestock production practices →Users acknowledge (i) increased recognition of environmental issues, (ii) guidance on policy and investment decisionsAn example from Northern Tanzania's dairy VC Focus = intensifying dairy systemsIn 3 distinct sites with 3 typical systemsIntervention package -changes in inputs and parameters• Clear environmental co-benefits• Overall improved efficiency• More absolute emissions with increase in land requirements in Muheza highland and Hai.• Potential reduction in total GHG emissions in Muheza lowland driven by improved feed efficiency and lower animal numbers • More land required in Muheza highland and Hai but less in Muheza lowland, driven by high yield of improved forages and reduced animal numbers as compared to baseline","tokenCount":"187"} \ No newline at end of file diff --git a/data/part_6/0491854619.json b/data/part_6/0491854619.json new file mode 100644 index 0000000000000000000000000000000000000000..be5c7cf4649dfcccabc7fa06576798d0f5cf9470 --- /dev/null +++ b/data/part_6/0491854619.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"8e1df8c1431e5208feb1c79744e27618","source":"gardian_index","url":"https://www.resakss.org/sites/default/files/Biennial%20Review%20Brief_Eswatini.pdf","id":"-391217267"},"keywords":[],"sieverID":"4a224ed5-2efa-4bac-a72f-4ca5fb9b2b0b","pagecount":"8","content":"Established in 2006 under the Comprehensive Africa Agriculture Development Programme (CAADP), the Regional Strategic Analysis and Knowledge Support System (ReSAKSS) supports efforts to promote evidence-and outcome-based policy planning and implementation. In particular, ReSAKSS provides data and related analytical and knowledge products to facilitate CAADP benchmarking, review, and mutual learning processes. AKADEMIYA2063 leads the work of ReSAKSS in partnership with the African Union Commission, the African Union Development Agency-NEPAD (AUDA-NEPAD), and leading regional economic communities (RECs).1  Introduction T he 2014 Malabo Declaration outlines Africa's vision for accelerating agricultural growth and transformation on the African continent through seven broad commitments from 2015 to 2025. The commitments include: (1) upholding the principles and values of the Comprehensive Africa Agriculture Development Programme (CAADP), (2) enhancing investment finance in agriculture, (3) ending hunger in Africa by 2025, (4) reducing poverty by half by 2025 through inclusive agricultural growth and transformation, (5) boosting intra-African trade in agricultural commodities and services, (6) enhancing the resilience of livelihoods and production systems to climate variability and other related risks, and (7) ensuring mutual accountability to actions and results by conducting a continent-wide biennial review (BR) to monitor progress in achieving the seven commitments. As part of fulfilling commitment 7 (mutual accountability), the second (2019) BR report and Africa Agriculture Transformation Scorecard (AATS) were launched at the 33rd African Union (AU) Summit in February 2020. This brief highlights Eswatini's performance in the second BR and assesses challenges faced and lessons learned by the country during the review process. The brief also reviews policy and programmatic changes in Eswatini that can be attributed to the first (2017) and second BRs. It concludes by highlighting required policy actions for Eswatini to implement to meet the Malabo Commitments by 2025.For the second BR, the benchmark (minimum score for a country to be on track in implementing the Malabo Declaration commitments) was 6.66 out of 10 (AUC, 2020). Even though Eswatini did not meet the benchmark, the overall score indicates an increase of 5 percent in the country's performance compared to the first BR. As shown in Table 1, Eswatini performed well below the benchmark and the poor performance is attributed to the country's poor performance in all the thematic areas. On the other hand, the country performed better than Southern African Development Community (SADC) middle-income countries as well as SADC countries as a whole, on average, in 3 thematic areas (themes 3, 4, and 7) (Table 1). For theme 5, however, Eswatini performed below SADC low-income countries, on average. The areas of very weak performance included the commitment on agriculture finance (score of 3.27 compared to the minimum score of 10) and a commitment on boosting intra-Africa trade and services (score of 1.39 compared to the minimum score of 3). In comparison to the first BR, the country also regressed in themes with already weak performanceby 59 percent on theme 2 and 10 percent on theme 5 (Table 2). Conversely, Eswatini saw notable improvements on 5 of the 7 commitments compared to the first BR, namely: recommitment to the CAADP process; ending hunger by 2025; halving poverty through agriculture; enhancing resilience to climate change, and commitment to mutual accountability for action and results as depicted in Table 2. While Eswatini reported on most of the BR indicators, some data gaps remain which the country needs to address in future BRs. Data quantity and quality challenges continued to affect the BR process, including in this round which was engulfed by several data gaps due to incorrectly compiled or uncompiled data. Data for the reported commodities were obtained through special studies conducted by consultants who were engaged by the Food and Agriculture Organization of the United Nations (FAO). Availability of data on post-harvest losses and climate change resilience remained a challenge. Furthermore, given that Eswatini is still in the process of developing a trade-in service strategy, data on trade-in services could not be obtained for reporting purposes.The following actions taken by Eswatini contributed to the country's success during the second BR: Participation at regional BR training workshop and dissemination of BR report within the country. Stakeholders also reported on and reviewed the (National Agriculture Investment Plan) NAIP annually.An independent technical review report was submitted to the Ministry evaluating the implementation of NAIP.The projects related to NAIP were funded and the funds allocated were reflected in the national budget. These helped to ease the implementation of projects, particularly regarding accountability and transparency and enhanced reporting to the portfolio committee.Public expenditures to agriculture increased as compared to the reported figures in the first BR and more resources were made available for emergency relief activities, including for drought.The ministry of agriculture began to pilot the commercialization of Swazi Nation Land Bill to enable households to secure their land rights.Overall, the second BR report shows that Eswatini is not on track to achieving the Malabo commitments by 2025. This is a setback for the country because in the first BR, the country's overall score was above the minimum benchmark, indicating that the country was on track to achieving the Malabo targets. Moreover, the country is still quite a long way regarding putting in place policies to attract its youth into agricultural value chains and increasing spending for agriculture research and development as a share of GDP. Eswatini needs to implement recommendations emanating from the second BR to ensure there is progress on the commitment areas for which it did not do well, while still focusing on areas where the performance was satisfactory.The following recommendations are important for Eswatini to improve implementation of the Malabo commitments and to get back on track for the next BR:There is a need to develop or update the national plan for implementing the Malabo declaration using the CAADP implementation approach, including an inclusive and participatory approach to facilitate the Eswatini CAADP Process. Eswatini needs to expedite the process of developing a NAIP that is compliant with the Malabo Declaration commitments and also prepare a NAIP implementation progress report. The second generation of NAIPs (NAIP 2.0) is quite imperative for Eswatini to attain the Malabo Declaration targets. Continuous Stakeholder engagement is also necessary to maintain the CAADP momentum.Eswatini should focus on increasing the proportion of men and women engaged in agriculture with access to financial services.There is a need to increase total agricultural research spending as a share of agricultural GDP.This will offer useful insights into relative levels of agricultural research and development investment needed in Eswatini.Eswatini is known to have weak land tenure security and obtained poor scores on this indicator. Therefore, the country needs to invest more in strengthening the land tenure rights of land users or owners to increase the proportion of farm households with ownership or secure land rights.There is also a need to improve and develop efficient monitoring and evaluation and data management systems in Eswatini as well as harmonise all the BR data into the Central Statistics Office. The country needs to align its indicators and targets with those of the BR process to avoid duplication and to minimise discrepancies.In-order to foster the principles of ownership and mutual accountability in the agriculture sector, there is an urgent need to improve the coordination of Eswatini's BR report validation processes. Presenting the country BR report to parliament-the agriculture portfolio committee-and to the cabinet can help to generate buy-in and ownership of the process at the highest level of political leadership.Lastly, Eswatini should ratify the African Continental Free Trade Area (AfCFTA) to enhance and strengthen bilateral agricultural trade. ","tokenCount":"1247"} \ No newline at end of file diff --git a/data/part_6/0492760927.json b/data/part_6/0492760927.json new file mode 100644 index 0000000000000000000000000000000000000000..58b00c5ec463d97423b88a8fcf9eb1086d3a0170 --- /dev/null +++ b/data/part_6/0492760927.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"69aedef24ca8f2dc5ecbdc831e566af9","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/27efea15-e577-4cd5-8424-4cc346911079/retrieve","id":"-1744816883"},"keywords":["climate change","adaptation","agriculture practices"],"sieverID":"dd51599b-4538-4744-a8d0-fa5f6138b670","pagecount":"49","content":"e Segurança Alimentar (CCAFS). Maputo, Moçambique. Disponível on-line no site: www.ccafs.cgiar.org. Publicado pelo Programa de Pesquisa de CGIAR sobre Mudanças Climáticas, Agricultura e Segurança Alimentar (CCAFS). Os relatórios de CCAFS tem como objetivo difundir os trabalhos de pesquisa e práticas sobre mudanças climáticas, agricultura e segurança alimentar e estimular o feedback da comunidade científica. O Programa de Pesquisa de CGIAR sobre Mudanças Climáticas, Agricultura e Segurança Alimentar (CCAFS) é uma parceria estratégica de CGIAR e o Centro Internacional de Agricultura Tropical (CIAT). Este programa é realizado com os fundos dos doadores do CGIAR, a Agência Dinamarquesa de Desenvolvimento Internacional (DANIDA), Agência Australiana para o Desenvolvimento Internacional (AusAID), ajuda Irlandeza, Fundos do Ambiete de Canada, Ministério dos Negócios Estrangeiros dos Países Baixos, Agência Suíça para o Desenvolvimento e Cooperação (SDC), Instituto de Investigação Científica Tropical (IICT), Ajuda da Inglaterra, Governo da Rússia, a União Europeia (UE), Ministério dos Negócios Estrangeiros e do Comércio da Nova Zelândia, com o apoio técnico do Fundo Internacional para o Desenvolvimento Agrícola (FIDA).Moçambique é tido como um dos países que vai sofrer mais os efeitos negativos das mudanças climáticas, através de uma maior intensificação dos fenómenos de secas, cheias e ciclones. O distrito de Xai-Xai devido à sua localização geográfica no litoral e na foz de um dos grandes rios de Moçambique e da África Austral, o rio Limpopo, irá sofrer frequentes ciclones e cheias. Mas também há população que pratica agricultura na zona alta, no sistema de sequeiro, que poderá sofrer das irregularidades das chuvas. Estes eventos climáticos poderão prejudicar a maioria da população do distrito pelo facto de depender de actividades vulneráveis às mudanças climáticas, nomeadamente agricultura, pecuária e exploração de recursos florestais. Por outro lado, a maioria dos produtores tem um fraco acesso à informação e infraestruturas básicas para o desenvolvimento de actividades de adaptação às mudanças climáticas.Como forma de apoiar esta população nos seus esforços de adaptação aos efeitos das mudanças climáticas, o Instituto de Investigação Agrária de Moçambique (IIAM) e o Centro Internacional de Agricultura Tropical (CIAT), no âmbito do Programa CCAFS (Mudanças Climáticas, Agricultura e Segurança Alimentar), desenharam um projecto designado \"Gerindo o risco climático para melhorar o modo de vida e a capacidade de adaptação das famílias rurais nos ecossistemas agrários no sul de Moçambique\", para os distritos de Xai-Xai e Chicualacuala, província de Gaza.Este diagnóstico surge no âmbito deste projecto e visa analisar os problemas, os conhecimentos e as necessidades dos produtores potenciais beneficiários na área de adaptabilidade aos efeitos das mudanças climáticas de modo a desenhar intervenções que respondam às suas necessidades. A recolha de dados da pesquisa baseou-se em três diferentes abordagens, nomeadamente: (i) o questionário dirigido aos agregados familiares (AFs); (ii) a discussão com grupos focais constituído por membros das associações de produtores e; (ii) entrevistas semi-estruturadas dirigidas aos líderes comunitários, lideres das associações de produtores e agentes de extensão agrária baseados nos povoados estudados. No total foram inquiridos 166 agregados familiares, quatro grupos de discussão constituídos por quatro a 12 pessoas e, entrevistados 10 informantes chave.Os resultados da pesquisa mostram que a população dos quatro povoados do distrito de Xai-Xai pesquisados tem muitas lacunas na aplicação das práticas agrícolas com destaque para o controle de pragas e doenças no milho, mandioca, feijão nhemba, hortícolas, citrinos, cajueiros e mangueiras; melhoramento da fertilidade de solo e conservação da humidade de solo; cultivo de variedades melhoradas tolerantes à seca; compassos culturais e épocas de sementeiras recomendadas. No que concerne às actividades de pecuárias, os AFs têm fraco domínio das práticas de maneio alimentar e sanitário nas principais espécies existentes. No maneio dos recursos florestais, há um fraco domínio de todas as práticas que visam o melhoramento, conservação e aproveitamento dos recursos florestais. Os AFs também têm poucos conhecimentos das matérias de processamento de produtos agrários e conservação de milho, feijão nhemba e batata-reno. Os produtores têm igualmente constrangimentos de natureza financeira para o acesso e utilização das novas tecnologias agrárias.As estratégias identificadas para uma maior capacidade adaptativa aos efeitos das mudanças climáticas incluem a organização de treinamentos e demonstrações das melhores práticas agrárias com desataque para as práticas que demandam pouco investimento financeiros para o seu acesso e utilização. Similarmente, vai-se apostar na produção de informação radiofónica sobre os conhecimentos agrários para sua vinculação na rádio comunitária, com o objectivo de sensibilizar os produtores a adoptar práticas que contribuem para uma melhor adaptação e resiliência do modo de vida perante os efeitos das mudanças climáticas. Moçambique, devido a sua localização geográfica, é apontado como um dos países que vai sofrer mais os efeitos negativos das mudanças climáticas, nomeadamente secas, cheias e ciclones (Mosquito et al., 2009;MICOA, 2005). Os impactos das mudanças climáticas serão agravados devido à limitada capacidade humana, institucional e financeira que Moçambique tem para antecipar e responder directa ou indirectamente os seus efeitos (MICOA, 2005).As regiões Sul e Centro de Moçambique são apontadas como sendo as que sofrerão mais os efeitos negativos das secas, cheias e ciclones (MICOA, 2006) , 2005). Estes eventos climáticos poderão prejudicar a maioria da população do distrito pelo facto de esta população depender de actividades que são muito vulneráveis aos efeitos das mudanças climáticas, tais como agricultura, pesca e exploração de recursos florestais. Estes efeitos serão ainda mais agravados devido ao fraco acesso à informação e infraestruturas básicas para o desenvolvimento da capacidade adaptativa da população aos efeitos das mudanças climáticas (Matavel, 2012).Para minimizar os impactos das mudanças climáticas previstos no distrito de Xai-Xai, o projecto de investigação sobre adaptação às mudanças climáticas implementado pelo IIAM em colaboração com CIAT, pretende realizar acções de pesquisa e disseminação de tecnologias assim como práticas de produção agrária que melhor se adaptem às mudanças climáticas e às condições de produção locais. De acordo com Swanson (1991) e Boydell & Leary (2003) as acções de transferência de tecnologias, incluindo as de pesquisa para transferência de tecnologias poderão ser eficazes caso sejam antecedidas por um diagnóstico dos problemas, e das necessidades dos produtores alvos da intervenção.Este diagnóstico conduzido em quatro povoados do distrito de Xai-Xai surge como meio para assegurar que: (i) os conhecimentos e a informação a oferecer aos produtores respondam às preocupações e limitações dos beneficiários (Boydell e Leary, 2003); (ii) a interacção entre a investigação, extensão e produtores, que é fundamental para adopção das tecnologias, seja boa (Swanson, 1991); (iii) não haja sobreposição das acções; (iv) o corrente projecto aprenda das lições de projectos anteriores do mesmo âmbito implementados no distrito de Xai-Xai.O presente relatório de pesquisa apresenta a descrição das práticas agrárias, de maneio dos recursos florestais, de processamento de frutas, assim como descreve as tecnologias disseminadas e as potenciais barreiras de adopção das tecnologias. O relatório também apresenta as principais áreas de necessidades de formação e informação para os potenciais beneficiários do projecto no distrito do Xai-Xai. Analisar os problemas, os conhecimentos e as necessidades dos potenciais produtores beneficiários da intervenção na área de adaptabilidade às condições de mudanças climáticas de modo a desenhar intervenções que respondam às suas reais necessidades.1) Identificar as lacunas existentes em termos de informação, conhecimentos e tecnologias agrárias (agricultura, pecuária e recursos florestais) nas comunidades do distrito de Xai-Xai para melhor responder aos efeitos das mudanças climáticas. 2) Descrever as potenciais barreiras que podem interferir na participação dos beneficiários nas intervenções de disseminação das tecnologias e no acesso e utilização dos conhecimentos a serem disseminados. 3) Identificar as estratégias de intervenção para responder às lacunas identificadas;O trabalho de recolha de dados para este diagnóstico privilegiou a combinação dos métodos quantitativos e qualitativos. A combinação dos dois métodos visa fundamentalmente suprir as fraquezas e/ou aproveitar as forças de cada método (Denscombe, 2007). Para os métodos quantitativos usou-se o questionário administrado aos agregados familiares (AFs) e nos métodos qualitativos recorreu-se às entrevistas semiestruturadas com os informantes chaves e discussão com grupos focais. As técnicas usadas neste diagnóstico são também sugeridas pelo Swanson (1991), como sendo as recomendáveis para a fase de diagnóstico dos problemas, necessidades e sistemas de produção locais dos produtores.Com o questionário recolheu-se a informação sobre as características dos potenciais beneficiários das formações, práticas agropecuárias, o nível de domínio dos conhecimentos relevantes para a mitigação dos riscos das mudanças climáticas, e o interesse em aprender essas práticas por parte dos potenciais beneficiários. As entrevistas semiestruturadas e as discussões com grupos focais foram usadas para recolher informação geral sobre: as características dos sistemas de produção locais, as principais fruteiras e as práticas usadas para o processamento e conservação das frutas; os principais constrangimentos nas actividades agrárias; as estratégias a adoptar para a disseminação das tecnologias agrárias e; os factores que poderão influenciar as actividades de transferência e divulgação de tecnologias agrárias.Este diagnóstico foi conduzido em quatro povoados pertencentes a três postos administrativos do distrito de Xai-Xai. O distrito encontra-se no extremo sul da província de Gaza e é limitado ao Sul pelo Oceano Índico, a Norte pelos distritos de Chibuto e Chókwé, a Este pelo distrito de Bilene e a Oeste pelo distrito de Mandlakazi (Ministério de Administração Estatal, 2005; UNEP/FAO/PAP/MICOA, 1998). O distrito possui uma população recenseada em 2007 de 212.459 habitantes. Estes eventos climáticos poderão prejudicar as actividades agropecuárias do distrito principalmente por que a maior parte dos produtores desenvolvem as suas actividades ao longo do vale do rio Limpopo.O diagnóstico foi conduzido em quatro povoados selecionados para a implementação do projecto no distrito de Xai-Xai. Os quatro povoados possuem 19 bairros e um total de 5.576 agregados familiares (Tabela 1).Para a recolha de dados quantitativos optou-se pela estratégia de amostragem probabilística estratificada por área geográfica, nomeadamente povoado e bairro. Esta estratégia visava fundamentalmente garantir que seja recolhida informação de todos os povoados beneficiários desta intervenção. Em cada povoado, foram seleccionados de forma aleatória, dois a quatro bairros, em função do número de agregados a inquirir. Dentro dos bairros foram selecionadas aleatoriamente os quarterões e nos quarterões os agregados familiares à inquirir.O tamanho de amostra para o inquérito foi de 166 agregados familiares. Este tamanho de amostra foi calculado com base na fórmula recomendada para casos em que a variável mais importante de estudo é nominal ou ordinal e a população de estudo é finita 1 . Foi considerado para o cálculo da amostra, o nível de confiança de 95% e margem de erro de 5%.O inquérito conseguiu cobrir em 100% o número de amostra planificado. A maioria dos inquiridos é de Posto Administrativo de Chicumbane (49%) seguido de Chongoene (36%). Os inquiridos do posto administrativo de Zonguene representam 15% do total do universo da amostra do estudo (Tabela 1). Em termos de sexo dos inquiridos, a tabela 2 mostra que a maioria dos inquiridos é de sexo feminino (78%). Quanto à idade dos inquiridos, esta varia de 18 a 83 anos, com uma média de 43 anos e a mediana é de 40 anos. Para as entrevistas com os grupos focais, foi organizado um grupo focal em cada povoado. Portanto, foram organizados quatro grupos focais compostos por 4 a 14 produtores membros de associações de produtores. A maioria dos participantes dos grupos focais era de sexo feminino (Tabela 3). A tabela 5 mostra o número e percentagem dos agregados familiares que praticam actividades agropecuárias, com ênfase para as áreas de intervenção do corrente projecto no distrito de Xai-Xai.Quase todos AFs dedicam-se à agricultura (99,4%) e cerca de 63% tem a pecuária como uma das suas actividades principais. Em relação às actividades florestais, menos 6% dos AFs fazem corte e venda de lenha e estacas. Apenas 0.6% dos AFs estão envolvidos na produção e venda de carvão. A maior percentagem dos AFs envolvida na exploração dos recursos florestais é constituída por aqueles que fazem a recolha e venda de frutas silvestres e outros produtos não madeireiros (13%). O resultado da localização das machambas pode-nos ajudar a perceber que a questão das inundações das áreas de produção no vale do Limpopo afectará a maioria da população, cerca de 80% da população, dos quais 20% tem machamba apenas na zona baixa e os restantes têm em ambas as zonas.Mas também em casos de fraca precipitação, as áreas de produção da zona alta, onde cerca de 79% dos AFs produz, poderão também sofrer. Portanto, nas estratégias de alívio aos efeitos das mudanças climáticas no distrito de Xai-Xai devem-se direcionar as suas acções tanto para as questões de inundações e cheias, como para casos de secas.A tabela 7 apresenta o número de AFs membros e não membros de alguma organização de produtores e a localização das suas parcelas de produção, se estão apenas na zona baixa ou apenas na zona alta e se têm machambas em ambas as zonas. Os resultados mostram que dos 166 AFs inquiridos nos povoados do distrito de Xai-Xai, apenas 15% são membros de alguma organização de produtores. A maioria dos produtores associados assim como não-associados têm pelo menos uma parcela na zona baixa. Esta maioria representa 88% dos associados (16% têm parcelas apenas na zona baixa e 72% em ambas as zonas) e aproximadamente 80% dos não associados (21% têm machambas apenas na zona baixa e 59% têm machambas em ambas as zonas). Estes resultados de associativismo deixam claro que a maioria dos produtores de Xai-Xai não faz parte de associações. Mas por outro lado, os resultados nos dizem que não existem grandes diferenças entre os associados e não associados em termos da localização das suas machambas. Isto implica que nas intervenções, tanto da zona baixa assim como da zona alta, deve envolver os dois grupos de produtores.A figura 1 mostra a preferência por culturas dos AFs dos povoados estudados no distrito de Xai-Xai (percentagem dos AFs que pratica as principais culturas). A cultura mais praticada é o milho (98%), seguida da mandioca (90%), batata-doce (78%), feijão nhemba (83%), amendoim (80%) e hortícolas (57%). Um segundo grupo de culturas praticadas é constituído por abóbora (43%) e o feijão jugo (41,0%). As culturas cultivadas por menos de 15% de AFs são a batata-reno, o arroz e a melancia. A mapira e mexoeira não são cultivadas no distrito de Xai-Xai. As seguintes culturas, milho, batata-doce e hortícola são mais praticadas na zona baixa, ao longo do vale do rio Limpopo (Figura 2), e o arroz só se cultiva na zona baixa. A mandioca, o feijão nhemba e amendoim são maioritariamente cultivadas na zona alta, no sistema de sequeiro.Figura 2: Culturas praticadas na zona baixa, vale do LimpopoA Tabela 8 apresenta as principais práticas culturais que são utilizadas nas diversas culturas, nomeadamente a preparação da terra, sementeira, sacha e colheita e o período do ano em que acontecem. Duma forma geral, o distrito de Xai-Xai tem duas épocas agrícolas que correspondem às sementeiras de inverno e verão. A maioria das sementeiras ocorre entre Setembro e Dezembro, onde são semeadas seis das nove principais culturas praticadas nomeadamente, milho, mandioca, batatadoce, hortícolas, feijão nhemba e feijão jugo. O outro período pico das sementeiras é de Abril a Junho, com a sementeira de cinco das nove culturas, nomeadamente milho, batata-doce, amendoim, batata reno e hortícolas.As hortícolas são as únicas que são feitas durante todo o ano, no sistema de regadio. O milho e a batatadoce são cultivados nas duas épocas agrícolas.Em relação às sachas, os períodos de maior actividade correspondem aos meses de Outubro a Fevereiro e de Maio a Junho. As colheitas ocorrem ao longo de todo o ano (Tabela 8). As culturas praticadas durante todo ano e as que são semeadas nas duas épocas agrícolas, são fundamentais na estabilidade alimentar por providenciar produtos em mais do que um período do ano. Para as outras culturas que são praticadas em apenas uma época agrícola, devem ser adoptadas estratégias de armazenamento ao nível do AF, de modo a assegurar a sua disponibilidade para a família ao longo do ano. O sistema de cultivo mais usado pelos produtores locais é o de consociação de culturas, com excepção de batata-doce, que tem sido cultivada no sistema de cultivo puro. Mas há casos em que a batata-doce é cultivada nas bordaduras do campo. As consociações mais frequentes são: mandioca com amendoim; mandioca com feijão jugo e milho; milho com feijão nhemba.Para avaliarmos a aplicação das práticas de cultivo que podem ajudar a reduzir a vulnerabilidade das comunidades aos efeitos das mudanças climáticas, os inquiridos foram questionados sobre 16 práticas ligadas ao melhoramento da fertilidade de solo, controlo de pragas e doenças, conservação da humidade de solo, uso de variedades melhoradas e uso de compasso e densidade adequados para cada tipo de cultura e condições de cultivo.A tabela 8 apresenta o número de AFs que usaram ou não, nos últimos três anos, cada uma das 16 práticas agrícolas. A maioria das práticas agrícolas apresentadas não foi aplicada pelos AFs dos povoados inquiridos no distrito de Xai-Xai. Menos de um quarto dos inquiridos respondeu que tinha usado as seguintes práticas: aplicação de adubos; preparação e aplicação de composto; aplicação de pesticidas sintéticos; preparação e aplicação de pesticidas naturais; lavoura mínima; uso de variedades melhoradas tolerantes à salinidade, e pousio melhorado. Entre 25% a 49% dos inquiridos responderam que tinham usado as seguintes práticas: aplicação de estrume para melhorar a fertilidade do solo; culturas de cobertura, variedades melhoradas tolerantes à seca e consociação de árvores/arbustos com culturas anuais (Tabela 9). Uso de prática de preparação da terra para o cultivo através do sistema de corte e queima 48 28,9% Cultivo em linha usando os compassos e densidade recomendada para cada tipo de cultura 99 59,6%Fonte: Dados do inquérito aos AFs colectados pelos autores Contudo, mesmo para as práticas em que mais da metade dos inquiridos responderam que tinham aplicado nos últimos três anos, nem todas foram realmente aplicadas. Isto porque na triangulação com informação das entrevistas semiestruturadas e nos trabalhos com grupos focais percebeu-se que: (i) as pessoas que responderam que fazem cobertura morta na verdade se referem à incorporação dos restos de colheita e capim na altura de lavoura; (ii) o cultivo em linhas usando os compassos e densidade recomendada, é feito principalmente nas hortícolas. No milho também é feito, mas só quando a lavoura é feita com recurso à tracção animal e/ou com tractor, meios esses aos quais a maioria da população não tem acesso.Questionados sobre porque não faziam a cobertura morta, os entrevistados de Poiombo e Aldeia 3 de Fevereiro afirmaram que esta actividade demanda muito tempo adicional dos produtores, que podia ser usado para fazer outras actividades. Em relação ao cultivo em linha, não se conseguiu a explicação.Com base no trabalho com os grupos focais e entrevistas semiestruturadas com os informantes chave, foram apurados os principais constrangimentos que os agricultores encaram nas actividades agrícolas.Os constrangimentos encontrados estão relacionados com o fraco acesso e domínio de informação e conhecimentos agrícolas pelos produtores. A seguir apresentam-se os principais constrangimentos por povoado do distrito de Xai-Xai: Perda da produção na zona alta devido à escassez e irregularidade das chuvas;  Deficiente conhecimento das técnicas de conservação de produtos agrícolas pós-colheita (batata reno e hortícolas);  Fraco conhecimento das práticas de controlo de pragas e doenças nas hortícolas principalmente o controle da lagarta na couve e repolho;  Limitado uso de sementes melhoradas;  Fraco domínio e aplicação da densidade e compassos recomendados para cada cultura. Perda de produção devido a ocorrência frequente de défices hídricos na zona alta e inundações na zona baixa;  Incerteza das épocas de sementeira devido à variabilidade climática (épocas das chuvas) trazendo como consequência a fraca produção na zona alta;  Assoreamento da vala principal de drenagem e consequentes inundações dos campos;  Deficiente conhecimento das técnicas de rega e drenagem, causando inundações em áreas reservadas para produção agrícola;  Deficiente e fraco uso de adubos orgânicos e inorgânicos;  Fraco domínio das dosagens de pesticidas;  Redução do rendimento nas culturas de: (i) milho, devido ao escaravelho preto e gafanhotos;(ii) arroz e feijão nhemba, por causa da praga de escaravelho preto.  Pragas e doenças nas hortícolas principalmente no tomate e couve (principalmente afídios);  Perdas pós-colheita no milho e feijão nhemba devido à ocorrência de pragas de armazém como o gorgulho, e fraco domínio das práticas do seu controlo;  Erosão das encostas devido à remoção da cobertura vegetal para a prática da agricultura pela população; Baixos preços de venda no mercado devido à fraca capacidade de negociação;  Saturação do mercado de hortícolas no período fresco. Secas cíclicas e inundações frequentes dos campos de produção (zona baixa), que contribuem para a perda da produção;  Incidência de diversas pragas de campo nos tubérculos e no milho;  Incidência de pragas e doenças nas hortícolas particularmente na couve, alface e repolho;  Perdas de produção pós-colheita devido a pragas de armazém, principalmente o gorgulho nos cereais;  Assoreamento das valas de drenagem. Assoreamento das valas de drenagem, dificultando o movimento das águas e provocando inundações;  Cheias e secas cíclicas prejudicando as culturas;  Perdas de rendimento das culturas de: (i) milho, devido à lagarta invasora, broca do colmo, escaravelho preto, gafanhoto elegante, míldio e listrado; (ii) couve e repolho, devido a lagartas das folhas; (iii) tomate e batata reno, por causa de murcha bacteriana; (iv) alho, por causa de ferrugem, e; (v) mandioca, devido ao gafanhoto elegante.  Perdas da produção de milho no armazém devido, principalmente, ao ataque de gorgulho.Os constrangimentos aqui apresentados mostram-nos que a produção agrícola é afectada principalmente por questões relacionadas com a variabilidade da precipitação, pragas e doenças nas principais culturas praticadas, uso de semente não melhorada, baixa fertilidade de solo, compasso e densidade de plantação inadequados e inundação dos campos de produção (zona baixa).As principais espécies de animais criadas no distrito de Xai-Xai são os bovinos, caprinos, suínos e aves (galinhas e patos).A Figura 3 apresenta a proporção dos AFs nos povoados inquiridos, que possuem cada uma das principais espécies de animais. Neste gráfico, pode-se ver que as aves e os caprinos são as espécies mais comuns nos AFs de Xai-Xai, sendo criadas por 67% e 47% dos AFs, respectivamente. Os bovinos e os suínos existem em 25% e 24% dos agregados familiares inquiridos, respectivamente. Quanto aos suínos, provavelmente existiam muito mais criadores antes da peste suína africana que assolou o distrito de Xai-Xai meses antes desta pesquisa. Para perceber quais as estratégias de maneio pecuário usadas pelos AFs nos últimos três anos para fazer face à variabilidade climática, foram sugeridas no questionário nove práticas de maneio, descritas na Tabela 10. Duma forma geral, há um fraco uso destas práticas de maneio pela maioria dos AFs de Xai-Xai. Apenas em uma das nove práticas (identificação de doenças mais frequentes nos animais), houve mais de metade dos inquiridos que aplicaram nos últimos três anos. O isolamento dos animais doentes do resto da manada (aplicada por 44% dos AFs) e o tratamento de doenças mais frequentes nos animais (aplicada por 33% dos AFs), constituem as práticas mais aplicadas pelos criadores inquiridos nos povoados deste distrito. As restantes práticas foram aplicadas por menos de 25% dos AFs inquiridos (Tabela 10). Em Chicumbane, os constrangimentos identificados são similares aos levantados nos povoados de Nhocuene e Poiombo com a excepção dos abcessos que ocorrem nas cobaias (cavia porcellus). A carência do pasto, que afecta os bovinos e caprinos num total de cerca de cinco meses ocorre nos períodos de Janeiro a Fevereiro, devido à inundação da zona baixa onde a maioria dos criadores pasta os seus animais; e de Agosto a Outubro, devido à seca que reduz a disponibilidade e qualidade de pasto nesse período (Tabela 12). Para além das duas razões acima apresentadas relacionadas ao período do ano, a escassez de pasto é também apresentada como sendo causada pela redução das áreas de pastagens devido ao aumento de número de criadores na zona, expansão da área residencial e das actividades agrícolas do projecto Uambau.A diarreia nos caprinos ocorre nos meses de Junho e Julho. A doença de sarna nos bovinos, caprinos e coelhos, tem-se registado no período de Junho a Agosto. A peste suína africana, que tem causado mortes massivas de animais ocorre com maior frequência nos meses de Abril e Dezembro. As doenças de newcastle, nas galinhas, e hepatite vírica, nos patos, apresentam uma maior prevalência nos meses de Junho, Julho e Dezembro. As cobaias sofrem de abcessos principalmente de Agosto a Outubro (Tabela 12). A maioria das frutas é colhida no período entre Dezembro a Fevereiro, ondem amadurecem pelo menos cinco das nove fruteiras existentes nos povoados estudados. O período mais pobre em frutas é correspondente aos meses de Agosto a Novembro. Nestes meses só se colhe entre um a dois tipos de frutas das fruteiras mencionadas. Isto nos sugere a seguinte pergunta: será que nos períodos de maior abundância da fruta ela é totalmente aproveitada? Será que as comunidades têm algumas estratégias de processamento e conservação da fruta para permitir o seu consumo diversificado e em períodos de escassez?Nas entrevistas com os informantes chave e nos trabalhos com grupos focais, constatou-se que estas frutas alistadas na tabela 14 são maioritariamente consumidas sem nenhum processamento e também não existe uma estratégia para a sua conservação. A excepção vai para o caju que é usado para a preparação de sumo de caju e bebidas alcoólicas.As razões apresentadas de não processamento e conservação das frutas exóticas estão ligadas ao não domínio dessas práticas pela comunidade dos povoados estudados. Encontramos apenas um caso dos membros da Associação Ntlawa Wa Djondzo Yaku Vikela Ndala, baseada na Aldeia a Voz da Frelimo, na localidade de Zonguene, que aprendeu a fazer sumos e jams a partir de frutas. Alguns membros têm preparado estes subprodutos da fruta de uma forma regular.As frutas nativas mais comuns no distrito de Xai-Xai são o canhu, mapfilwa, tindziva, nheva, nula, ata silvestre, massala e tâmaras. O canhu, mapfiwa, tindziva e a massala existem em todos os povoados estudados. As outras frutas são comuns em apenas um dos povoados. Nula (em Poiombo); Tamara (em A Voz da Frelimo) ou em dois povoados; nheva (em Nhocuene e Poiombo); e ata silvestre (em Chicumbane-sede e Aldeia a Voz da Frelimo) (Tabela 15).A maioria das frutas amadurece no período entre Novembro e Abril. Neste período amadurece três a quatro espécies de frutas. Os meses de Junho e Julho, não se colhe nenhuma fruta nativa e no período de Agosto a Outubro pode-se comer apenas a massala (Tabela 16). Fonte: Dados da discussão com grupos focais, colectados pelos autores Duma forma geral, o período entre Dezembro e Fevereiro é a época de maior abundância de fruta nestas comunidades. Isto pelo facto de das 17 frutas exóticas e nativas mais comuns nas quatro comunidades estudadas, colher-se entre nove a 11. Entre os meses de Julho e Outubro há grande escassez de fruta, tanto das fruteiras nativas assim como das fruteiras exóticas.Tal como registado nas frutas exóticas, as nativas também são consumidas maioritariamente em fresco, sem processamento e conservação, com excepção de canhú [Sclerocarya birrea (A. Rich) Hochst.Sbsp. Caffra] e massala (Strychnos spinosa Lam.) que também são usadas para a preparação de bebidas alcoólicas.A seguir são apresentados, por povoado beneficiário do projecto no distrito de Xai-Xai, os principais constrangimentos encarados pelos AFs na produção e processamento das frutas nativas e exóticas. Os constrangimentos identificados dizem respeito ao acesso e domínio da informação e conhecimentos ligados ao maneio de fruteiras e processamento das frutas. Em todos os povoados apenas foram apesentados problemas ligados as frutas exóticas, provavelmente devido à limitada disponibilidade de florestas para se explorar as espécies nativas. Ataques severos de afídios nos citrinos;  Baixa produtividade dos cajueiros, devido ao problema de oídio;  Declínio do rendimento das mangueiras e citrinos devido ao apodrecimento das suas frutas antes de amadurecimento.Povoado de Chicumbane-sede  Incidência de pragas e doenças nos citrinos e cajueiros;  Perda de muita fruta no pico de amadurecimento por falta de mercado para a venda da fruta fresca e fraco domínio das técnicas para o seu processamento e conservação. Incidência de pragas e doenças nas laranjeiras (cochonilha e mosca da fruta) e nos cajueiros (oídio);  Perda de grandes quantidades de manga no pico de amadurecimento por falta de mercado para a venda da fruta fresca e fraco domínio das técnicas para o seu processamento e conservação.A incidência de pragas e doenças nas fruteiras e o fraco domínio das práticas de processamento são os dois problemas que afectam as três comunidades entrevistadas. Como vimos anteriormente, a maioria das fruteiras atingem a maturação no período de Dezembro e Fevereiro, o que pode significar excedente de fruta nesta época. Ai que surge a necessidade de processamento e conservação de fruta neste período, não só como uma estratégia de diversificação das formas de consumo mas, principalmente, como um meio de aproveitamento da fruta, de acréscimo de valor e de contribuição para a renda familiar.Foi inquirido aos AFs sobre qual o seu nível de domínio sobre as 15 práticas de produção agrícola, apresentadas no questionário, que podem minimizar os efeitos do risco climático (Tabela 17). Os resultados mostram que mais de 50% dos inquiridos responderam que não tinham domínio em 11 das 15 práticas apresentadas.As práticas com mais agregados familiares (mais de 80%) que não dominam (nenhum ou fraco domínio) são as seguintes: aplicação de adubos e pesticidas sintéticos; preparação e aplicação de pesticidas naturais; preparação e aplicação de pesticidas naturais; e captação e conservação de água das chuvas na machamba (ex. sulcos espaçados, micro-bacias, sulcos fechados, etc.). Na segunda posição, em termos de número de pessoas que não dominam, (entre 60% a 80%) encontramos: preparação e aplicação de composto orgânico; lavoura mínima; uso de variedades melhoradas tolerantes à seca; pousio melhorado usando espécies de rápido crescimento e tolerante à seca (ex. feijão bóer variedade gigante, leucaena, etc.). E na terceira posição temos consociação de árvores/arbustos de crescimento rápido com culturas anuais, com 51% de AFs que não dominam (Tabela 17). Em três práticas de produção agrícola, a maioria dos inquiridos respondeu que tinha bom a muito bom domínio. Essas práticas são as seguintes: (i) uso de cobertura morta, (57%); (ii) cultivo em linha usando os compassos e densidade recomendada para cada cultura (62.6%) e; (iii) rotação de culturas (68%). Em uma prática, uso de culturas de cobertura, metade dos inquiridos disseram que não tinham domínio e outra metade disse que dominava.Cruzando os resultados de aplicação das práticas agrícolas (Tabela 9) e o domínio dessas práticas pelos produtores de Xai-Xai (Tabela 17), pode se ver que os produtores aplicavam o que responderam que dominavam. Portanto, há constrangimentos apontados que podem ser resolvidos por algumas das práticas aqui indicadas, mas que eles não aplicam por razões provavelmente relacionadas com o fraco domínio tecnológico das mesmas. Contudo, não se pode ignorar a provável existência de outros factores que possam influenciar a não aplicação de certas práticas e que não tenham sido apurados neste estudo.A Tabela 18 apresenta o nível de domínio pelos AFs, das nove práticas de maneio pecuário indicadas no questionário deste estudo. Em todas as práticas de maneio pecuário, registou-se mais de 50% dos inquiridos que responderam que não tinham domínio (nenhum ou pouco domínio).As práticas de maneio pecuário em que mais AFs (mais de 80%) responderam que não dominam são: conservação de forragem em feno; conservação e tratamento de resíduos agrícolas da machamba para alimentação do gado na época de escassez de pasto; uso de blocos multinutritivos e suplementação de ruminantes na época seca; cultivo de árvores forrageiras resistentes à seca; retenção/colheita e conservação de água da chuva para o abeberamento do gado; e construção de currais melhorados (p. ex. curais elevados para cabritos, ovelhas). A prática de isolamento dos animais doentes do resto da manada (principalmente com brucelose e tuberculose), com 61% dos inquiridos que não dominam, está na segunda posição. O terceiro grupo é constituído pela prática de identificação das doenças mais frequentes nos animais, em que 53% dos AFs responderam que não tinham domínio (Tabela 18). Aqui vamos apresentar o nível de domínio das práticas de maneio de recursos florestais pelos AFs de Xai-Xai. Das seis práticas de maneio de recursos florestais inquiridas, constatou-se que a maioria dos AFs não tem domínio (nenhum e pouco domínio). Para todas as práticas de maneio florestal, mais de 80% dos inquiridos responderam que não tinham domínio (Tabela 19). Para avaliação do domínio das práticas de processamento de produtos agrárias pelos agregados familiares foram usados nove tipos de agroprocessamento, ilustrados na Tabela 20. Duma forma geral há um fraco domínio das práticas de processamento pela maioria dos AFs. A percentagem dos AFs que respondeu que tinha bom a muito bom domínio de cada prática, não ultrapassa os 21%, com excepção da produção de farinha de mandioca em que cerca de 69% dos inquiridos respondeu que tinha bom a muito bom domínio. Das oito práticas que a maioria dos inquiridos respondeu que não tinha domínio, podemos subdividir em dois grupos. O primeiro grupo é constituído por sete práticas, em que mais de 80% dos inquiridos disseram que não tinham domínio. As sete práticas que constituem o grupo das que mais pessoas não sabem como processar são: processamento de frutas em sumos, jam e conservante a seco; preparação de manteiga a partir da amêndoa de canhú; processamento de batata-doce (em sumos, jam, bolos, biscoitos, etc.); preparação de sumos, bolos, biscoitos e conservante seco a partir de hortícolas; e processamento de caju em melaço. O segundo grupo é constituído pelo processamento de leite de vaca/cabrito em iogurte, em que 75% dos AFs alegam não terem domínio (Tabela 20). Estes resultados reforçam as constatações registadas na descrição dos constrangimentos do maneio de fruteiras em que as comunidades indicaram que perdiam muita produção no pico da maturação de muitas frutas devido ao fraco domínio das práticas de processamento da fruta para o aproveitamento daquela fruta que não é consumida e/ou não encontra o mercado.Neste capítulo pretendemos identificar as áreas em que os AFs estão interessados em receber alguma informação e conhecimento. As áreas usadas para esta avaliação são as mesmas anteriormente analisadas no capítulo de avaliação do nível domínio e de aplicação das práticas agrícolas, pecuárias, florestais e de agroprocessamento e conservação de produtos agrários.A Tabela 21 descreve o nível de interesse dos AFs, em aprender as práticas agrícolas. Das 15 práticas alistadas na tabela 21, a maioria dos AFs dos quatro povoados do distrito de Xai-Xai envolvidos no estudo, mostrou muito interesse em aprender todas as práticas com excepção do uso de variedades melhoradas tolerantes à salinidade, onde 47% de AFs responderam que tinham muito interesse. Para as restantes práticas, entre 55% a 90% dos AFs, responderam que tinham muito interesse em aprender. As práticas que registaram percentagens mais altas, mais de 80% dos AFs com muito interesse de aprender, são as seguintes: aplicação de adubos e pesticidas sintéticos; aplicação de estrumes de animais; preparação e aplicação de composto orgânico e de pesticidas naturais, uso de variedades melhoradas tolerantes à seca e pousio melhorado usando espécies de rápido crescimento e tolerantes à seca (ex. feijão bóer, variedade gigante; leucaena, etc.). O segundo grupo de práticas com maior aceitação para sua implementação, entre 60 -80% dos AFs muito interessados, é constituído pelos seguintes conhecimentos: rotação de culturas, consociação de árvores/arbustos de crescimento rápido com culturas anuais e lavoura mínima. A prática de captação e conservação de água das chuvas na machamba (ex. sulcos espaçados, micro-bacias, sulcos fechados, etc.) está no terceiro grupo, com 55% dos AFs.Estes resultados de interesse conjugados com os de domínio e aplicação das práticas agrícolas pelos agregados familiares dizem-nos que há necessidade e interesse em disseminar todas as práticas de produção agrícolas sugeridas para adaptação às alterações climáticas no Xai-Xai, com excepção da prática de uso de variedades melhoradas tolerantes a salinidade. Nesta prática a maioria dos inquiridos não tem nenhum domínio mas também não está interessada em aprender. Isto pode significar que o problema de salinidade ainda não constitui uma grande preocupação na maioria das zonas do distrito de Xai-Xai, com excepção de Zonguene onde aparentemente o problema é mais sério.Com base nas entrevistas e discussão em grupos focais, os produtores acresceram e/ou reforçaram a lista das áreas em que estão interessados em receber treinamentos. As áreas ou os tópicos que os produtores disseram que têm interesse em aprender são os seguintes: Compassos e densidades culturais no milho e hortícolas diversas.  Uso de herbicidas com ênfase nas dosagens;  Épocas ideais actualizadas para a sementeira de diversas culturas, tendo em conta as mudanças ocorridas com as alterações climáticas;  Práticas de conservação da humidade de solo;  Técnicas e estratégias de melhoramento da fertilidade de solo;  Controle de pragas nas culturas de milho, hortícolas e mandioca.A tabela 22 apresenta o nível de interesse dos AFs em aprender as práticas de maneio pecuário. Em sete das nove práticas de maneio pecuário alistados na tabela, a maioria dos AFs (52% a 95%) respondeu estar muito interessado em receber os conhecimentos sobre as respectivas práticas.As práticas como identificação de doenças mais importantes e isolamento de animais doentes do resto da manada são as que mais de 80% dos AFs mostraram ter muito interesse em aprender. A construção de curais melhoradas está no segundo lugar em termos de número de AFs interessados, com 72% de AFs. O terceiro grupo de práticas que teve muitos AFs interessados (entre 50% -59%) é constituído pelos seguintes conhecimentos: conservação de forragem em feno; conservação e tratamento de resíduos agrícolas na machamba para alimentação do gado na época de escassez de pasto; uso de blocos multinutritivos e suplementação de ruminantes na época seca; e cultivo de árvores forrageiras resistentes a seca (Tabela 22). O fraco interesse em aprender as práticas de retenção e conservação da água deve-se ao facto de o distrito possuir recursos hídrios permanentes e semipermanentes. Em relação ao fraco interesse em aprender as práticas de tratamento das doenças, o mais provável é que este trabalho é feito pelos promotores. E os produtores acham que quem deveria aprender é este grupo que assiste os criadores.Duma forma geral, os AFs demonstraram interesse em aprender todas as práticas que anteriormente responderam que não tinham aplicado e não dominavam as formas de sua aplicação. Para além das áreas descritas na tabela 21 acima, os produtores disseram-nos que estavam interessadas em receber os conhecimentos sobre a desparasitação dos caprinos e bovino.Em cinco das seis práticas usadas para avaliar o nível de interesse dos AFs de Xai-Xai em aprender as práticas de maneio de recursos florestais a maioria dos inquiridos disse estar muito interessada em aprender os seus conhecimentos (Tabela 23).A prática com mais AFs interessados em aprender é a de cultivo de plantas medicinais, com 92%. Em segundo lugar estão as práticas sobre colecção e tratamento ou pré-tratamento da semente de árvores florestais de espécies nativas e apicultura, com percentagens de 64% e 60% respectivamente. As práticas sobre estabelecimento e gestão de viveiros florestais de espécies nativas e implantação e maneio de florestas com espécies nativas estão no terceiro grupo dos conhecimentos que a maioria dos AFs está muito interessado em aprender. A percentagem dos inquiridos com muito interesse é de 57% para os viveiros e 55% para a implantação e gestão de florestas (Tabela 23).A única prática que teve menos de metade dos inquiridos interessados em aprender é o cultivo de pasto e forragem na floresta, que registou 49% de agregados familiares dos povoados inquiridos, no distrito de Xai-Xai (Tabela 23). A intervenção no âmbito de transmissão dos conhecimentos sobre colecção e tratamento da semente, assim como estabelecimento e maneio de viveiros florestais pode impulsionar os trabalhos de produção de mudas de espécies florestais para o cumprimento da decisão presidencial \"um líder uma floresta\". Isto porque por exemplo, o líder comunitário de Chicumbane disse que para responder a decisão de criação de florestas comunitárias, as comunidades dependem das plantas disponibilizadas pela Direcção Provincial da Agricultura, que não são suficientes para responder às necessidades.A tabela 24 abaixo apresenta o resumo das respostas dos AFs de Xai-Xai em relação ao seu nível de interesse em aprender cada uma das nove práticas de processamento sugeridas no corrente diagnóstico. Em todas as práticas, a maioria dos AFs respondeu estar muito interessado em aprender.As práticas com maior número de inquiridos interessados (mais de 80%) são: processamento de frutas em sumos, jam e em conservante a seco; preparação de manteiga a partir da amêndoa de canhú; processamento de batata-doce (em sumos, jam, bolos, biscoitos, etc.); preparação de sumos, bolos, biscoitos de hortícolas; processamento de hortícolas em conservante seco; e processamento de caju em melaço. O segundo grupo é composto por duas práticas nomeadamente: processamento de leite de vaca/cabrito em iogurte (73%) e produção de farinha mandioca (65%) (Tabela 24). Nas entrevistas com informantes chave e nas discussões nos grupos focais, os produtores também manifestaram interesse de aprender as práticas de conservação pós-colheita de milho e feijão nhemba, principalmente para evitar o gorgulho e rato que casam grandes perdas da produção destas duas culturas.A Tabela 25 mostra as tecnologias agrárias disseminadas nos postos administrativos de Chonguene, Chicumbane e Zonguene nos últimos três anos. A introdução de muitas tecnologias agrárias indicadas teve em conta a necessidade de se fazer face aos efeitos da seca e cheias (ou ambos). Outras tecnologias foram focalizadas ao aumento da renda e segurança alimentar e nutricional das famílias.Tendo em vista a redução do impacto da seca foram introduzidas as práticas de produção de ananás, mandioca e batata-doce, e as hortas caseiras em Nhocuene e Poiombo. Em Poiombo também foi introduzido o cultivo da variedade de tomate HTX14 que é relativamente mais tolerante à seca. Em Poiombo foi ainda introduzida a prática da agricultura de conservação, uma prática que também foi experimentada no povoado da Voz da Frelimo. Destas práticas apenas a agricultura de conservação é que não está a ser aplicada por razões que não conseguimos apurar.Em Poiombo e Nhocuene a Visão Mundial também focalizou as suas acções na disseminação de práticas de gestão integrada das pragas e doenças, tanto na zona baixa como na zona alta. Em Zonguene a Visão Mundial também introduziu novas culturas de cenoura, pimento e beringela, enquanto em Poiombo introduziu o cultivo de batata reno. Estas práticas e culturas continuam sendo aplicadas, contudo as pragas e doenças continuam a ser preocupação dos produtores. Isto porque, nestas comunidades, há ainda um fraco domínio dos princípios básicos de seu controlo.Visando melhorar a actuação dos produtores, a Visão Mundial capacitou os produtores de Poiombo e Nhocuene na gestão de produção e preparação dos planos de negócio. Alguns dos informantes chave destes povoados referiram que esta capacitação melhorou a actividade agrária dos produtores.Em Poiombo, os Serviços Distritais das Actividades Económicas (SDAEs) introduziram a piscicultura como uma actividade alternativa às actividades agrárias tradicionais. Nhocuene, Poiombo e Chjicumban e-Sede Tecnologia de processamento de arroz e uso de multi-cultivadoras especialmente na produção do arroz.Projecto de reabilitação da Barragem de Massingir Poiombo Fonte: Dados dos grupos focais e entrevistas semiestruturadas aos informantes chaves, colectado pelos autores Para reduzir o impacto da erosão hidrica que muitas vezes ocorre durante o tempo chuvoso e a erosão eólica causada pelos ventos fortes que ocorrem com alguma frequencia na zona de Chicumbane, foi introduzida a prática de plantação de mudas de eucalipto e casuarinas. Esta actividade de plantação de árvores aconteceu apenas com apoio das instituições públicas locais. A liderança da comunidade local mesmo sabendo da importância desta acção, diz que não tem como reagir porque não tem mudas e nem tem pessoas, no povoado, preparadas para fazer os viveiros.A visão Mundial e os Serviços Distritais das Actividades Económicas (SDAEs) desenvolveram acções de consciencialização das famílias sobre a necessidade de praticar actividades agrárias tanto na zona baixa como na zona alta como forma de assegurar que a falha de produção na zona baixa devido às inundações poderia ser compensada pela produção na zona alta e vice-versa.Na perspectiva de assegurar maior e melhor aproveitamento dos produtos agrícolas, a Save the Children, em coordenação com os Serviços Distritais das Actividades Económicas introduziram o processamento da batata-doce de polpa alaranjada e mandioca aos membros da associação de produtores do posto administrativo de Zonguene. A Visão Mundial também capacitou os produtores de Poiombo e Nhocuene em processamento de batata-doce de polpa alaranjada. Mas de uma forma geral há fraca aplicação destas práticas de processamento introduzidas. As pessoas destas comunidades alegam as dificuldades financeiras para a compra dos ingredientes necessários e a falta de mercado para a comercialização dos produtos processados como as principais razões da fraca aplicação.A extensão agrária e o IIAM introduziram a estratégia de melhoramento e maneio da fertilidade de solo através da preparação e aplicação de composto nos povoados de Poimbo e a Voz da Frelimo. Esta prática não foi adoptada pelo facto de requerer tempo adicional dos produtores.Em relação às estratégias de disseminação das tecnologias a Tabela 25 mostra que a maioria das tecnologias e práticas agrícolas foram apresentadas aos produtores em secções de treinamento intensivos de curta duração, um a cinco dias, e através de demonstração baseadas nas técnicas de escola na machamba do camponês e campo de demonstração de resultados. Segundo as organizações intervenientes no processo de disseminação, estes métodos foram mais preferidos porque usam os princípios que ajudam a ter maior motivação e participação dos produtores no processo de aprendizagem.Foram identificadas algumas barreiras que podem interferir na participação dos beneficiários nos eventos de capacitação e no acesso e utilização dos conhecimentos disseminados. As referidas barreiras incluem aspectos sociais e culturais, disponibilidade de tempo e recursos necessários para o acesso e utilização das novas tecnologias agrárias, entre outros aspectos.No distrito de Xai-Xai foi indicado que os horários para a participação em potenciais eventos de capacitação dos produtores devem ser seleccionados de forma cautelosa uma vez que regra geral os produtores estão nos campos de produção, individuais ou da associação, até cerca das 13 horas, embora em alguns casos continuem as suas actividades até ao final da tarde.Algumas práticas agropecuárias têm baixa probabilidade de serem adoptadas. Este é o caso das práticas de pousio e rotação de culturas que embora sejam até certo ponto conhecidas e reconhecido o seu benefício, não são realizadas porque alguns produtores só tem uma machamba, o que não facilita a sua execução; Esta situação é mais acentuada em Nhocuene e Poiombo onde uma grande parte das áreas da zona alta que eram destinadas à prática das actividades agropecuárias foi destinada à habitação como consequência das inundações que geralmente ocorrem no distrito de Xai-Xai, especialmente na zona baixa, reduzindo drasticamente as áreas de produção e limitando a possibilidade de realizar o pousio e a rotação de culturas. Em Chicumbane-Sede, os produtores perderam grande parte dos seus campos, na zona baixa, a favor da Empresa Chinesa Uambau que explora uma grande área do Vale do Limpopo.Práticas que impõem um esforço físico adicional ao produtor ou práticas em que se exigem a sua comparticipação financeira têm menos probabilidade de serem adoptadas. Este é o caso de por exemplo, das pulverizações contra o oídio, dos banhos carracicidas e dos diversos insumos agrícolas. Os banhos carracicidas tendem a ser cada vez menos regulares porque os criadores de gado devem comprar os produtos necessários. Para os insumos agrícolas tais como a semente melhorada, adubos e pesticidas, os produtores não têm usado na maioria dos casos devido aos preços de compra que são altos. As poucas vezes que eles usam é nas hortícolas ou quando estes são disponibilizados a custo zero.No povoado de Poiombo foi apresentado uma limitação relacionado especificamente à limitação da área de produção de arroz devido à limitada capacidade de aquisição de equipamento para fazer as sachas e mondas. Esta questão está relacionada com a disponibilidade local destes equipamentos.O Quadro 8 apresenta as organizações locais envolvidas na disseminação de informação e conhecimentos agrários, no distrito de Xai-Xai. Fazem parte desta lista, os Serviços Distritais das Actividades Económicas de Xai-Xai, as casas agrárias de Nhocuene e Poimbo, Visão Mundial, associações de produtores, Empresa do Regadio de Baixo Limpopo, Rádio Comunitária de Xai-Xai, Empresa Chinesa Uambau e Centro de Formação da Associação WutomiAgri.Os SDAEs de Xai-Xai é uma instituição do Estado que coordena as actividades económicas no distrito, incluindo actividades agropecuárias. Os SDAEs estão baseados no posto administrativo de Chonguene e tem extensionistas nos quatro povoados onde este projecto das mudanças climáticas está em implementação.As casas agrárias de Nhocuene e Poimbo localizadas nos bairros que ostentam os mesmos nomes são outras organizações de grande interesse no processo de disseminação de informação e conhecimentos agrários. As casas agrárias permitem a articulação entre os SDAEs, através dos extensionistas, e os produtores. É nas casas agrárias onde se vendem insumos diversos (fertilizantes, sementes, pesticidas, etc.), equipamentos e instrumentos de produção. As casas agrárias também possuem condições para organização de eventos de formação tais como salas de aulas e campos para as aulas práticas e demonstrações diversas (Figura 4). Em Chonguene funciona a organização não-governamental Visão Mundial, que através do seu Projecto de Segurança Alimentar dá assistência técnica agrária aos produtores locais através de treinamentos, demonstrações e disponibilização de insumos agrários, e dinamiza esquemas de poupança comunitária.No distrito de Xai-Xai existe uma empresa designada por Empresa do Regadio de Baixo Limpopo, que funciona em coordenação com os SDAEs, prestando apoio na abertura e limpeza das valas de drenagem. Esta empresa também esteve envolvida na capacitação dos extensionistas e das casas agrárias. Portanto, os trabalhos de capacitação comunitária nas áreas ligadas ao maneio de água de rega deveriam ser feitos com envolvimento desta empresa.Os produtores para além de estarem organizados em casas agrárias também estão em associações de produtores. As associações de produtores são organizações locais de produtores envolvidos em actividades agropecuárias. Estas organizações permitem o apoio interno entre os seus membros e facilitam a assistência técnica providenciada pela extensão agrária. As associações de produtores funcionam como meio de disseminação de informação e tecnologias agrárias aos produtores não associados, desempenhando assim uma função social muito importante. Na área de implementação do projecto foram identificados três associações, sendo duas em Chicumbane e uma em Zonguene.A outra organização local relevante na disseminação de informação e práticas agrícolas é a rádio. A rádio é um dos meios de comunicação de massa encontrado no distrito de Xai-Xai. No distrito de Xai-Xai funciona a rádio local designada rádio comunitária de Xai-Xai mas a população de distrito também tem acesso a outras rádios com destaque para a antena nacional da Rádio Moçambique. A rádio comunitária de Xai-Xai é um órgão de comunicação muito importante na actividade agropecuária. A rádio comunitária é muito utilizada pelo sector da Agricultura local para a veiculação de informação relacionada com a campanha agrícola, aparecimento de surtos, vacinação de gado e situações de cheias e secas. A rádio comunitária constitui um espaço para a difusão de informação e tecnologias agrárias mesmo em locais onde a extensão agrária não tem alcance directo.Na baixa de Chicumbane existe a empresa Chinesa Uambau, uma organização nova que desenvolve actividades agrárias no distrito de Xai-Xai. No âmbito da responsabilidade social, espera-se que esta empresa venha capacitar as comunidades de Chicumbane, que perderam as suas parcelas de produção, em matérias de produção agrícola, com destaque para a produção de pasto e fenação.Ainda no povoado de Chicumbane, existe uma instituição consagrada a formação agrária designada por Centro de Formação da Associação WutomiAgri. Esta é uma organização que se dedica à formação de jovens na área de produção agrária e empreendedorismo. Tem condições para acolher as formações e outros eventos de transferência de tecnologias nomeadamente salas de aulas, alojamento, campos para as práticas e técnicos/professores efectivos. As informações são transmitidas às populações durante as reuniões. Estes eventos têm sido momentos para diálogo entre as lideranças locais e a população e têm sido aproveitados para debater diversos aspectos da vida comunitária.A rádio é outro recurso muito privilegiado na disseminação de informação e interacção a nível local dado que muita população possui aparelhos de rádio (receptores). A disseminação de informação é feita através da Rádio Comunitária de Xai-Xai, sediada na cidade de Xai-Xai. A Rádio Moçambique (RM) é igualmente muito utilizada na disseminação de informação.O uso da rádio para a veiculação de informações carece de pagamento e os horários e língua de veiculação são acordados entre os organismos interessados e a rádio. A produção de programas de rádio pode ser feita em conjunto com os técnicos da rádio, em estúdio ou fora dele, observados os aspectos logísticos. A rádio também pode veicular spots produzidos por outras instituições. O Sector da Agricultura tem-se socorrido das duas instituições (Rádio Comunitária de Xai-Xai e RM delegação de Gaza) para difundir diversas informações, especialmente sobre vacinações de animais, ocorrência de surtos, entre outras. As instituições de gestão de calamidades também são um exemplo de organismos que usam a rádio comunitária para difundir informações de interesse das populações locais especialmente no âmbito de cheias e secas.A televisão é um outro recurso usado para a veiculação de informação usado por diversos organismos, contudo, de importância relativamente menor comparativamente com a rádio uma vez que um limitado número de famílias possui aparelhos de televisão. Alguns bairros, como é o caso de Piombo, não possuem energia eléctrica. Mesmo nos bairros com a rede eléctrica, o acesso a energia eléctrica para algumas famílias é limitado.O uso de material escrito como cartazes, folhetos, brochuras, manuais, etc., é muito limitado dado que poucos produtores são capazes de ler e escrever. A produção ou reprodução de materiais impressos podem ser feitas na cidade de Xai-Xai onde são prestados diversos serviços, incluindo serviços de internet.A região de Xai-Xai tem acesso a rede de telefonia móvel (Vodacom, mCel e Movitel). Os telemóveis duma forma geral, ainda não são aproveitados para a disseminação de informação agrária. Mas é uma oportunidade que devia ser considerada.No plano de acção resultante desta pesquisa, foram considerados os instrumentos já usados no distrito para a disseminação de informação de práticas agrícolas, principalmente as que achamos que podem ser efectivos nas acções propostas (anexo 1).5.1 Quais são as principais fontes de renda e subsistência dos agregados familiares e até que ponto essas actividades são vulneráveis as alterações climáticas?Quase todos agregados familiares dos quatro povoados de Xai-Xai diagnosticados dedicam-se a agricultura e mais de metade estão envolvidos na criação de animais e cerca de 13% estão envolvidos na exploração de recursos florestais. Segundo Plano Estratégico de Desenvolvimento do Distrito de Xai-Xai (2010), as actividades agro-pecuárias e a pesca de pequena escala constituem as principais fontes de rendimento e subsistência das famílias principalmente camponeses do distrito de Xai-Xai. Em relação aos recursos florestais, há poucas famílias que exploram devido, fundamentalmente, a escassez de florestas próximo das comunidades. A exploração dos recursos florestais se concentra na recolha de frutas silvestres e outros recursos não madeireiros, como a lenha para servir de combustível e estacas para a construção. A escassez de recursos florestais poderá minar as actividades da maioria dos agregados familiares pois a lenha e o carvão vegetal são os principais combustíveis domésticos no distrito de Xai-Xai (Ministério Administração Estatal, 2005).Como se pode ver pelos dados acima, a população de Xai-Xai depende principalmente de actividades sensíveis às mudanças climáticas nomeadamente a agricultura, a pecuária, a pesca e a extracção de recursos florestais. Segundo os modelos de previsão de vulnerabilidade social, quanto maior for a população dependente dos recursos naturais, maior é o risco de vulnerabilidade as alterações climáticas (Matavel, 2012). Portanto, a população de Xai-Xai, dependendo da estratégia de adaptação que ela está a adoptar, poderá estar vulnerável às alterações climáticas porque as principais actividades de subsistência e renda são intimamente ligadas aos recursos naturais.Em termos de culturas, as mais praticadas são milho, mandioca, feijão nhemba, amendoim e batatadoce. Os animais domésticos mais importantes nos AFs dos povoados onde o projecto está em implementação são as aves, os caprinos, os bovinos e os suínos. Estas culturas e espécies de animais mais importantes são também descritas como as mais importantes para os AFs do distrito, no perfil do distrito, editado em 2005. As espécies comuns nos quatro povoados e que são importantes no fornecimento da fruta nativa são as seguintes: Canhú [sclerocarya birrea (A. Rich) Hochst. Sbsp. Caffra], Mapfilwa (Vangueria infausta Burch.), Tidziva (Dialium schlechteri Harms), Nheva (Manilkara discolor (Sond.) J.H. Hemsl.), Nula, Ata silvestre (Annona senegalensis Pers.), Massala (Strychnos spinosa Lam.), Tâmaras (Phoenix reclinata Jacq.).Apesar de em média os AFs terem três machambas, eles praticam o cultivo da maioria das culturas em sistema de consociação. Uma parte das principais culturas nomeadamente o milho e a batata-doce é semeada nas duas épocas agrícolas e as hortícolas são cultivadas durante todo o ano, aproveitando-se das condições de água existentes no vale do rio Limpopo.Nos povoados diagnosticados, a maioria dos produtores tem áreas de produção nas zonas altas e baixas, provavelmente para minimizar os efeitos tanto das inundações na zona baixa, que são muito frequentes nos últimos anos, como ilustram alguns relatórios (Asante, 2009; Ministério para a Coordenação da Acção Ambiental, 2006; Ministério para a Coordenação da Acção Ambiental, 2007), e a seca na zona alta que as populações também reportam como sendo um fenómeno importante nos últimos anos.Os agricultores das zonas inquiridas praticam agricultura nos moldes tradicionais, com fraca aplicação das práticas de controlo de pragas e doenças nas culturas, de práticas de melhoramento da fertilidade de solos e de conservação da humidade de solos. Há Também um fraco uso das variedades melhoradas que podem responder melhor a certas alterações climáticas. A fraca aplicação das práticas melhoradas deve-se a combinação de factores, nomeadamente: desconhecimento e/ou fraco domínio desses conhecimentos, nível de demanda de tempo adicional e recursos financeiros.Começando das pragas, vimos que os produtores têm tido perdas de produção no campo, principalmente nas culturas de milho, mandioca, feijão nhemba, hortícolas, citrinos, cajueiros e mangueiras e perdas pós-colheita, isto é, no armazém, devido a pragas e doenças. Isto pode estar associado a combinação de dois factores: o fraco domínio, uma vez que maioria dos inquiridos respondeu que não tinha conhecimentos sobre as praticas de controlo de pragas e doenças; a fraca capacidade de compra dos pesticidas, já que em alguns locais os produtores afirmaram que não aplicam práticas que demandam recursos financeiros adicionais. A questão de pragas vem reflectida também como preocupação dos produtores de Xai-Xai no perfil do distrito e na pesquisa conduzida pelo Marques et al. (2006). A incidência de pragas e doenças nas machambas localizadas no vale limpopo, segundo Marques et al. (2006), resulta de cultivo intensivo sem rotação de culturas. Com as mudanças climáticas este problema poderá ser mais frequente (MICOA, 2007;Cruz et al., n.d) e poderá contribuir para fracos rendimentos agrícolas.Há casos também de produtores que não aplicam algumas práticas agrícolas devido a importância que dão a cultura. Por exemplo, as poucas famílias que fazem alguma aplicação de adubos e fertilizantes nas suas machambas são as que se dedicam ao cultivo de hortícolas, provavelmente devido ao facto desta cultura ser de rendimento.Os produtores da zona alta não aplicam as práticas de conservação da humidade de solo como a cobertura morta, apesar de reclamarem de perdas de produção devido a seca porque alguns não dominam esta prática mas outros acham esta prática demanda mão-de-obra adicional, como foi referido pelos produtores de Poiombo e Chicumbane. E, os produtores das zonas baixas queixam-se das inundações frequentes das áreas de produção devido provavelmente ao fraco funcionamento das infraestruturas instaladas. Os modelos de circulação geral da atmosfera e, respectivas probabilidades associadas indicam uma previsão do aumento em mais de 25% o caudal do rio Limpopo e aumento da probabilidade de maiores picos de cheias neste rio nos próximos anos (Instituto Nacional de Gestão de Calamidades, 2009) e consequentemente no vale do Limpopo, onde se localiza as áreas de produção da maioria da população dos povoados estudados. A questão de estabilidade institucional e de infraestruturas públicas é um dos indicadores mais importantes de vulnerabilidade social (INGC, 2009, citado por Matavel, 2012) Há também um fraco uso de sementes melhoradas que poderão estar adaptadas as condições actuais resultantes do impacto das mudanças climáticas tais como a seca. Isto deve-se fundamentalmente ao facto de demandarem custos financeiros adicionais para os produtores.Na pecuária também há uma fraca aplicação das práticas de maneio alimentar e sanitárias, que ajudam na adaptação aos efeitos das mudanças climáticas. Por exemplo, os produtores apesar da redução da área de pastagem e a escassez do pasto devido a expansão da área residencial e as inundações e secas respectivamente, não aplicam as práticas de conservação de pasto e nem de aproveitamentos de resíduos agrícolas para alimentação do gado. Os produtores também não têm a hábito de fazer o tratamento das doenças nos seus animais apesar destes animais constituírem fonte de subsistência e em casos de renda do agregado familiar.Para a alimentação, há registos de carência de pasto principalmente na época seca (Julho a Setembro) em todos os povoados. Isto deve-se a seca e a redução da área de pastagem com o aumento da área residencial e conflito das áreas de pastagem com actividades agrícolas. Há também escassez de pasto na época chuvosa no povoado de Chicumbane devido a inundação da área de pastagem, uma vez que os animais são pastados maioritariamente no vale do rio Limpopo. Mas em contrapartida, a população deste distrito não está a usar as práticas de conservação de forragem em feno, e de resíduos agrícolas devido ao fraco domínio dos procedimentos para a preparação destas e de outras alternativas alimentares. Com a previsão do aumento da frequência das cheias (Instituto Nacional de Gestão de Calamidades, 2009), associada a expansão da área residencial e agrícola, tornará a actividade pecuária nas comunidades de Xai-Xai mais vulnerável as alterações climáticas. Portanto, é importante a adopção de alternativas alimentares de gado bovino e caprino no período de escassez do pasto.No que concerne as doenças, quase todos animais são frequentemente assolados por doenças típicas de cada espécie de animal. As doenças mais comuns nos quatro povoados estudados são: as diarreias, as sarnas, as diversas pestes, ferimentos nos cascos, abcessos e parasitas. Duma forma geral os criadores não têm dado cuidado sanitário aos seus animais. Segundo os extensioinitas, o maneio sanitário tem sido sempre relegado como da responsabilidade do governo e das ONGs, através dos promotores comunitários, que têm providenciado assistência a custo zero. Portanto, muitos criadores não estão dispostos a sustentar as despesas de tratamento dos seus animais. Mas por outro lado, os criadores não têm domínio das práticas de prevenção e controle das doenças dos principais animais criados nos povoados em pesquisa.Para os recursos florestais para além de serem escassos devido ao crescimento da área populacional, também não se registam iniciativas da própria população para o seu maneio sustentável. Olhando para aquilo que é a importância destes recursos uma vez que a lenha e o carvão são os principais combustíveis domésticos para a maioria da população do distrito de Xai-Xai, esperava-se uma maior reacção por parte dos agregados familiares para responder a esta problemática da redução das áreas florestais. Algumas iniciativas existentes são promovidas pelas ONGs e pelas instituições Governamentais e têm como foco a promoção das actividades de reflorestamento através de fornecimento de plantas e capacitação das comunidades no estabelecimento de viveiros comunitários de diversas espécies, com destaque para o mangal, casuarinas, eucaliptos e algumas fruteiras (Matavel, 2012).Outro elemento importante na adaptação as mudanças climáticas é a questão de aproveitamento da produção existente através do processamento, conservação e consumo. Duma forma geral, há um baixo nível de processamento dos produtos agrários. O fraco processamento tem causado perdas principalmente nas hortícolas, quando não se consegue vender toda a produção devido a saturação do mercado; na batata-doce, quando os produtores são forçadas a colher devido as inundações; nas frutas da época (nativas e exóticas), durante o pico da maturação, porque as famílias não conseguem consumir toda a produção. Por exemplo, todas as famílias têm mangueiras e quando é pico de maturação, ninguém consegue comer e nem vender, localmente, toda a sua produção. Nos produtos pecuários constatamos que, por exemplo, não há hábito de produzir iogurte com leite de vaca ou de cabrito.As principais razões de não processamento são o fraco domínio das técnicas de processamento e conservação dos produtos processados, a fraca capacidade financeira, para aquisição dos insumos necessários para o processamento e a alegada falta de mercado para a comercialização dos produtos processados. Este último factor é discutível porque os produtores não deram evidências de alguma tentativa de pesquisa de mercado.Das práticas que podem contribuir para adaptação aos impactos das alterações climáticas e que os produtores disseram que não tinham domínio, algumas eles estão interessados em aprender mas em outras não.Para a produção agrícola, os produtores estão interessados a aprender: (i) as diversas práticas de maneio de pragas e doenças nas culturas de milho, mandioca, feijão nhemba, hortícolas, citrinos, cajueiros e mangueiras; (ii) o melhoramento da fertilidade de solos com diversas práticas como a aplicação de adubos químicos, estrumes, preparação e aplicação de composto orgânico, pousio melhorado, consorciação de culturas com espécies arbóreas; (iii) uso de variedades melhoradas e tolerantes à seca, para os produtores da zona alta. Mas não estão interessados a aprender a usar variedades melhoradas tolerantes a salinidade. Isto pode ser um sinal de que este problema, com excepção de Zonguene onde já há evidência de existir solos com altos índices de salinidade, (Matavel, 2012) ainda não constitui uma grande preocupação.No que concerne a área pecuária, os criadores estão interessados em apresentar as práticas de maneio alimentar nomeadamente a conservação de forragem em feno, conservação e tratamento de resíduos agrícolas, uso de blocos multinutritivos e cultivo de árvores forrageiras. Mas este último tema, apesar de haver interesse poderá não ser muito prático para estes povoados devido a tendência de urbanização da maioria dos bairros. Em relação a componente sanitária, os produtores estão interessados em aprender a identificar as principais doenças e a aplicar a estratégia de isolamento dos animais doentes do resto dos animais. Mas eles acham que não precisam de aprender a tratar os animais, provavelmente porque eles assumirem que esta tarefa é da responsabilidade dos promotores sanitários.Para a área florestal, os agregados familiares estão interessados em aprender as práticas de maneio dos recursos florestais, provavelmente porque em alguns povoados já ressentem as dificuldades de acesso ao material para construção das suas casas, o combustível lenhoso e fruta nativa.Os produtores estão interessados também em aprender o processamento e conservação de todas as principais frutas (nativas e exóticas) e de todas as culturas, incluindo aquelas que não indicaram ter perdas de produção devido a lacuna de conhecimento do seu processamento. Os agregados familiares também estão interessados em conhecer as técnicas de produção de leite em iogurte.Cerca de metade dos inquiridos não sabe ler nem escrever na língua portuguesa e nem fala a língua portuguesa. Este dado pode constituir uma barreira para o acesso de informação quando esta é providenciada de forma escrita e também quando os meios de divulgação usam a língua portuguesa.A questão dos horários em que se organiza os eventos de disseminação dos conhecimentos, para casos de disseminação cara-a-cara, foi apresentado como sendo um aspecto importante a considerar, pelo facto de, regra geral, os produtores estarem ocupados nas suas machambas quase todos os dias, principalmente no período de manhã. Este facto acontece em maioria das famílias uma vez que elas têm em média três machambas e a maioria das actividades é feita manualmente. Portanto, é crucial negociar-se o período de encontros para a disseminação dos conhecimentos e o local para que os produtores tenham espaço para continuarem a exercer as suas actividades agrícolas.Quanto às práticas agrárias também foram identificadas algumas barreiras que podem interferir no acesso e adopção dos conhecimentos e tecnologias que podem melhor responder a adaptação as mudanças climáticas. Essas barreiras incluem as financeiras, tipo de tecnologia (se demanda trabalho adicional) e do acesso ao mercado para a comercialização da produção.Muitas práticas que demandam recursos financeiros adicionais para a sua aplicação, tais como, a compra de semente melhorada, o uso de pesticidas para o controle de pragas e doenças, os adubos químicos para a melhoria da fertilidade dos solos e o tratamento das doenças dos animais domésticos, poderão não ser facilmente acessíveis e usados pelos produtores de Xai-Xai. Esta afirmação é baseada no facto de as famílias não estarem por exemplo, a fazer a pulverização dos cajueiros contra oídio e a não levar o seu gado bovino para os banhos carracicida, mesmo sabendo a sua importância, apenas pelo facto de estes serviços implicarem o desembolso de valores monetários. Excepção vai para algumas actividades de rendimento, como é o caso de produção de hortícolas, onde há maior probabilidade de serem aplicadas as práticas que envolvem custo financeiros baseando no facto de as poucas pessoas que disseram que aplicavam pesticidas na sua machamba serem os produtores de hortícolas. E também pelo facto de durante a recolha de dados termos presenciado a compra de semente de hortícolas na casa agrária de Nhocuene.As práticas que demandam um esforço físico e tempo adicional para a sua aplicação também poderão não serem facilmente aceites pelos agricultores de Xai-Xai. Este é o caso de cobertura morta, conservação de forragem em feno e a conservação e tratamento de resíduos agrícolas para alimentação do gado. Isto porque, por exemplo, os produtores de Poiombo e Chicumbane terem afirmado que conhecem a prática de cobertura morta mas não aplicavam porque rouba muito tempo deles que poderia ser usado para outras actividades. Esta justificação de não se aceitar as práticas que adicionam tempo de trabalho das suas actividades pode vir pelo facto de eles terem já muito trabalho, uma vez que em médias cada AF tem três áreas de produção e a maioria das actividades agrícolas são feitas manualmente.As estratégias de processamento e conservação dos produtos agrários poderão não seradoptadas em grande medida se a sua disseminação não estar associada a análise e discussão sobre a cadeia de valor dos produtos eleitos. Isto porque os poucos produtores treinados em processamento de frutas diversas e batata-doce de polpa alaranjada têm apresentado a limitação de mercado e custo dos insumos para o processamento como um dos factores que os impede a aplicar os conhecimentos adquiridos. Se fizermos paralelismo com a experiência de compra de insumos para as hortícolas, pelo facto de ser uma cultura que tem o mercado e eles vêem os retornos do seu investimento, é possível também, para este caso, adquirir-se os insumos para o processamento.Considerações finais e recomendações 6.1 Considerações finais  A maioria dos agregados familiares são vulneráveis as mudanças climáticas em curso e/ou das previstas para o distrito de Xai-Xai, pelo facto de terem como fonte de subsistência e renda a agricultura, pecuária, pesca e exploração dos recursos florestais, actividades sensíveis aos fenómenos climáticos tais como secas e inundações. Este factor é agravado pelo fraco domínio, pelos produtores, dos conhecimentos de certas práticas que podem ajudar a adaptabilidade da comunidade produtiva aos novos cenários climáticos, associado ao fraco poder financeiro para aceder a algumas tecnologias e/ou a indisponibilidade de tempo e de recursos humanos para aplicar as práticas que demandam tempo adicional do produtor. As potenciais barreiras que podem interferir na participação dos beneficiários nas intervenções de disseminação das tecnologias e no acesso e utilização dos conhecimentos a serem disseminados são nomeadamente: o nível de escolaridade dos produtores, principalmente, o facto de a maioria não saber ler, escrever e falar a língua portuguesa, o que pode constituir uma barreira para o acesso de informação quando esta é providenciada de forma escrita e através de meios de comunicação que usam a língua portuguesa; os horários em que se organiza os eventos de disseminação dos conhecimentos, para casos de disseminação cara-a-cara, que devem ser compatíveis com as actividades diárias dos produtores; o tipo de tecnologia em termos se ela demanda custos financeiros adicionais para o seu acesso ou utilização, necessita de um esforço físico e tempo adicional significativo para a sua utilização. Em todos os povoados beneficiários do projecto, existem organizações de produtores que têm sido envolvidas em actividades de transferência de tecnologias. Portanto, estas organizações podem constituir potenciais parceiros locais na implementação de acções de divulgação e transferência de tecnologias agrárias. Para além destas organizações de produtores, podemos contar com o SDAE, a rádio comunitária de Xai-Xai e a Rádio Moçambique delegação de Gaza. Existem também ONGs que operam, nestes povoados, e que podem ser envolvidos no processo de transferência de tecnologias agrárias. Considerando as barreiras que os produtores têm para o acesso e utilização das tecnologias agrárias aconselháveis para melhor adaptação aos impactos climáticos esperados no distrito de Xai-Xai, recomendamos a sua capacitação em práticas culturais, incluindo práticas de baixo custo de maneio de pragas e doenças; uso de semente melhorada tolerante a seca; práticas de armazenamento de cereais e feijões de baixo custo; estratégias de maneio da fertilidade de solos com base nas práticas culturais e outras práticas que usam recursos localmente disponíveis. Recomendamos também a capacitação dos produtores em práticas de conservação de pasto e de aproveitamentos de resíduos agrícolas para alimentação do gado bovino e caprino na época seca; práticas de identificação das principais doenças e a aplicar as estratégias de isolamento dos animais doentes do resto dos animais para evitar a sua propagação. No processamento e conservação de produtos agrários, recomendamos a capacitação dos produtores em práticas de processamento e conservação de frutas (nativas e exóticas) e culturas diversas. Para o alcance de mais pessoas principalmente pessoas de baixo nível de escolaridade, recomendamos a produção de informação radiofónica sobre os conhecimentos agrários para sua vinculação na rádio comunitária, com o objectivo de sensibilizar os produtores a adoptar práticas que contribuem para uma melhor adaptação e resiliência do modo de vida perante os efeitos das mudanças climáticas. Recomendamos também a capacitação dos produtores e criadores em recursos financeiros, por via de crédito, para elevar a sua capacidade de acesso de outras práticas eficientes no que concerne ao acesso e utilização agrícola.","tokenCount":"12229"} \ No newline at end of file diff --git a/data/part_6/0493119783.json b/data/part_6/0493119783.json new file mode 100644 index 0000000000000000000000000000000000000000..ab5e0e64525d09860c0201cf5156ee3f3e03aab9 --- /dev/null +++ b/data/part_6/0493119783.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"d98d13462dd4fa0d07ebf04cde18ae15","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/dcd67843-95e0-4073-a5f0-225c85629381/retrieve","id":"-1363748383"},"keywords":[],"sieverID":"fb477d12-18ae-4839-bc10-6e7d148962f9","pagecount":"64","content":"CIAT encourages wide dissemination of its printed and electronic publications for maximum public benefit. Thus, in most cases, colleagues working in research and development should feel free to use CIAT materials for noncommercial purposes. However, the Center prohibits modification of these materials, and we expect to receive due credit. Though CIAT prepares its publications with considerable care, the Center does not guarantee their accuracy and completeness.Land degradation is a consistent loss of ecosystem functionality due to human and natural processes (Lal et al. 2012), or as defined by the United Nations Convention to Combat Desertification (UNCCD) a \"reduction or loss of the biological or economic productivity and complexity of rain fed cropland, irrigated cropland, or range, pasture, forest and woodlands resulting from land uses or from a process or combination of processes arising from human activities\" (UNCCD 2015c). Historically, it is a well-documented issue (Grove 1996, Beach et al. 2006, Ellis et al. 2013) and the degree of degradation has forced civilisations to adapt land management practises to the state of the environment, or abandon the landscape altogether (Costanza et al. 2007). Over the last four decades there has been an increase in human-induced land degradation and it is estimated to affect one third of global arable land (UNCCD 2015a, Vlek 2005) and to cost between USD 6.3-10.6 trillion annually or 10-17% of the world's gross domestic product (ELD 2015).Land degradation affects livelihoods, biodiversity and ecosystem services while it exacerbates climate change and ultimately impacts the well-being of 1.5 billion people globally (Lal et al. 2012, ELD 2015). The impacts are not evenly distributed across the globe, as approximately 40% of all land degradation occurs in the poorest countries, the least capable of mitigating and adapting to the impacts (UNCCD 2015b). Furthermore, as the impacts of land degradation have direct effects on both climate change and biodiversity loss (Lal et al. 2012) Acknowledging that land degradation is a global challenge and building on the international momentum to restore degraded lands (see for example Initiative 20×20 in Latin America and AFR100 in Africa), the Sustainable Development Goals (SDG) identified Land Degradation Neutrality (LDN) as an important component. While SDG 15 calls for the protection of terrestrial ecosystems and the fight against land degradation in general terms, target 15.3 explicitly formulates the vision of a \"land degradation neutral world\". After the political establishment of LDN in the context of the SDGs and the UNCCD, the challenge now becomes one of operationalization. First efforts to implement LDN at country level have been initialized already. In 2015, the UNCCD ran a LDN pilot project together with 14 countries 1 , which is now being followed by the LDN Target Setting Program (TSP) implemented by the UNCCD´s Global Mechanism. Following the invitation by the UNCCD COP to set voluntary LDN targets, so far more than 100 countries officially expressed their interest to participate in the TSP. The TSP aims at supporting countries in setting LDN targets, identifying strategies and measures to achieve these targets and establishing a corresponding monitoring scheme. It is expected that countries wishing to engage in the LDN process present their targets at COP 13 in late 2017.LDN target setting is a complex process that includes numerous political and technical aspects. The UNCCD´s LDN Technical Guide proposes a step-wise approach to define LDN targets and identify measures to achieve them (UNCCD 2016a). An integral part of any LDN target setting process is the assessment of a baseline, that means taking stock of the current land status. The LDN baseline is the basis for informed target setting and functions as a reference state for future monitoring. First attempts to develop national LDN baselines were undertaken during the LDN pilot phase in 2015.The objective of this report is to identify entry points and challenges for subnational LDN baselines in order to inform subnational planning processes as potential vehicle for the implementation of LDN targets on the ground. For this purpose two focus regions were chosen within two of the countries -namely Namibia and Costa Rica -that participated in the first LDN pilot phase. The focus areas in Namibia and Costa Rica are the regions of Otjozondjupa and Rio Jesus Maria watershed respectively. Both Namibia and Costa Rica provide interesting case studies given the differences in types of land degradation, national capacities, and land resources.The selection of methods for baseline assessement will have to take the subsequent monitoring of changes over time and space into consideration, as this is key for the detection of land degradation. Much care must be taken in choosing a methodology that ensure scientific rigor and quality of the assessment. It must quantify the impacts of human activities on the environment, as well as ensure better informed decision making through clear and concise communication of the current state of the land. Box 1 shows a checklist of important criteria for selection of land degradation assessment methodologies (UNCCD 2016a, UBA 2015, LDN Methodological Note [LDN-MN] 2015). These criteria will be explored in the following chapters in an analysis of available methods and data for sub-national baseline assessment.Box 1: Checklist of criteria for selection of baseline assessment methodologies. The indicator 'Land cover' is indicative of socio-ecological dynamics of management. Furthermore, this indicator is capable of identifying vulnerable transitions between natural and human-managed land cover classes. The indicators 'Land productivity' and 'Carbon stocks'2 Different technological aspects of LDN baseline mapping and monitoring are progressing at a relatively fast pace which resulted in some challenges during the writing of this report. For example, while the methods chapter (2) was completed by February 2016, changes were made in naming of the indicators (e.g. Land Cover / Land Use is now Land Cover) and general framework (e.g. the meaning of \"tier\" has changed). The authors have attempted to stay abreast of the changes in LDN vocabulary and recommendations regarding new datasets. However, some aspects of this report may become outdated as new datasets are published and improved satellite products become available to the wider public. This progress is a positive sign of the global commitment towards achieving a world that is land degradation neutral.measure the biophysical state, above ground NPP and below ground organic carbon content, of the different types of land cover. Both land productivity and soil organic carbon (SOC) stocks are directly affected by land management and are vulnerable to land degradation (LDN-MN 2015). 3The drivers of land degradation are highly complex socio-ecological processes working on multiple scales both in space and time and there has been debate about the appropriateness of the proposed indicators. Scientific literature supports this, as e.g. Geist and Lambin (2004), Wantzen andMol (2013), andSommer et al. (2011) all argue, a single set of cross-scale indicators are highly unlikely to accurately identify land degradation in different locations around the world (see also Box 2). Consequently, given the complexity of land degradation and as well as the range of capacities countries have, the TSP allows for local adaptations of these indicators and encourages the use of additional indicators that are relevant in the specific context (Buenemann et al. 2011, Sommer et al. 2011, LDN-MN 2015). The Technical Guide (UNCCD, 2016) also recommends different tiered datasets for developing LDN baselines and monitoring:• Tier 1: includes global and regional data (i.e. from earth observation systems)• Tier 2: refers to data from national statistics or national earth observation systems • Tier 3: are primary data from field surveys and ground measurements With regard to tier 1, UNCCD (2016a) has defined default global data sources with the aim to provide participating countries with globally available data for their validation and/or use in the absence of national data. These default data sources are included within the following review (chapter 2).As the word 'trend' implies land degradation monitoring is an analysis of changes over time. It is important to emphasize that in a baseline assessment, no time-series analysis is required as the assessment deals with the current natural resource stocks and state of the land.The following chapter focuses on available methods and data for assessing LDN baselines at the sub-national scale in Otjozondjupa Region in Namibia and Rio Jesús Maria watershed in Costa Rica, using available Tier 1 data. Chapter 3 will examine what Tier 2 and 3 data are available in the respective sub-national regions, as well as what alternative indicators might apply. It also covers the process of multi-criteria decision making process which was used in stakeholder workshops in both locations, to ensure. Lastly, we present the conclusions in chapter 4.This chapter gives an overview of remote sensing data sources and examines existing methodologies and datasets that can be used for developing baselines for LDN at subregional level using Tier 1 global datasets selected in accordance with the abovementioned checklist in Box 1.Land degradation assessments generally have been completed using several approaches, or combination of approaches (Sommer et al. 2010, Reed et al. 2011) The terms local, national, regional, and global scales are used throughout this report. The different scales refer to the management/institutional unit or the ecological scale it captures. There is no clear definition of the extent of the different scales, as it is highly contextual. For instance farm sizes vary from thousands of ha in Western Australia (can be captured by coarse scale data) to less than 0.5 ha subsistence farms in Tanzania (needs to be fine scale data to capture changes). The same is evident of municipal and country sizes around the world as well as ecosystem sizes and boundaries. Therefore, the appropriate scale of data reflect the scale of land management and the boundary of the underlying ecosystem (Willemen et al., 2009).radar waves are not restricted and they provide additional valuable information like surface roughness. However, few baselines exist, there is less expertise available in the land cover community, and passive optical systems are still the most widely used.The increased quality and availability of remote sensing data, including free satellite imagery such as MODIS and Landsat (e.g. the archives of the University of Maryland Global Land Cover Facility), and more recently Sentinel-2 of ESA's Copernicus program have opened a range of new possibilities for the application of remote sensing in the operational assessment of land degradation processes. The most commonly used free remote sensing sensors are listed below The explanatory value of remote sensing data can be increased by different modelling techniques. Modelling involves mathematical changes to values of the original data in order to capture features that are not directly measured by the data available, e.g. turning the points of soil samples into a continuous map, or when various remote sensing data layers are comprised into one map with multiple features.It might be necessary, for instance, to combine remote sensing data with field observations, or to improve on spatial and temporal resolution with ancillary remote sensing images (Congalton et al. 2014). MODIS of NASA's Terra and Aqua satellites, for example, produces a number of bands of different wavelengths that can detect multiple changes in the landscape with a high temporal resolution (see Table 2.1). However, many of these derived products are still only available at a coarse scale (500m and 1km), which challenges the assessment of land degradation because it often takes place at a finer scale.It is likewise possible to combine multiple remote sensing data into one analysis. For example, MODIS can provide a high temporal resolution, time series, allowing for analysis of seasonal patterns and phenology, while Landsat can provide better granularity to identify what processes and land uses underlie the situation. Remote sensing has been applied for the assessment of land degradation in several different contexts, most commonly for analysis of land cover and productivity, but also for assessing SOC and soil erosion prevalence in landscapes (see e.g. Eva et al., 2012;Vågen et al., 2013). 3) agricultural land (and artificial surfaces) change to natural and semi-natural land cover type.It is important to establish a solid baseline for these changes, as again, the context is central.There may be cases where highly degraded mining or agricultural lands are abandoned and the subsequent succession growth would be classified as semi-natural land cover. While these areas are still degraded when compared to the original land use, they may be classified as nondegraded when compared to the previous land use. This can be found in e.g. tropical rain forests, where cleared areas show quick succession after they are abandoned.A combination of approaches might be needed for detailed assessments of land cover baselines at a fine scale, as discussed above. It is advised (UNCCD 2015c(UNCCD , 2016a) that data used to establish a baseline should preferably be an average of a period of 10-15 years, and therefore, should be available from at least the year 2000 and onwards. The measurement unit should be in hectares, and the classification be based on Food and Agriculture Organization's (FAO) Land Cover Meta Language (LCML) is recommended to ensure global comparison is possible (UNCCD 2016a).The European Space Agency's (ESA) Climate Change Initiative Land Cover dataset (CCI-LC) (www.esa-landcover-cci.org) is the default tier 1 option for land cover set by UNCCD (UNCCD 2016a). This dataset is based on modeling of multiple remote sensing products (SAR, Landsat, MERIS, MODIS), has a 300m resolution, is publicly available, and is produced every five years since 1998, with the last updates in January of 2016. It classifies 22 different land cover categories. The spatial resolution can miss some of the detail important for sub-national assessments of land use changes (Ban et al., 2015), as for instance in small scale heterogeneous landscape, as shown in Figure 2.1. However, ESA's CCI-LC datasets were shown to be quite accurate at a global scale (74% accuracy) (Bontemps et al., 2015). Furthermore, it is still in production so will be available for future monitoring and evaluation (M&E) procedures, as well as be comparable between regions. This initiative was launched by China in 2010 with the aim to develop a set of land cover mapping products globally. The approach is a model that combines imagery from the 30m resolution Landsat, MODIS imagery, and SRTM digital elevation data (www.globeland30.org). Maps were produced for 2000 and 2010 (Error! Reference source not found.2.2) and thus have the recommended 10 year coverage for land cover baseline assessments. It is however, no longer updated and therefore will not be sufficient for future change detection. The GL30 is a publicly available at globallandcover.com.The land cover classes can be quite coarse for baseline studies at sub-national levels. GL30 is included in this review because it has a finer spatial resolution and better accuracy than ESA CCI-LC (300 m), and might be able to better capture the changes in small scale landscape mosaics. Figure 2.2 shows a 30m resolution land cover map for Namibia. Furthermore, GL30 is produced from a combination of multiple imagery and is an example of how additional spatial data can improve the final product, if spatial resolution is too coarse (Congalton et al. 2014), as discussed above 4 . The This spatial segmentation is then used to calculate on what type of land cover change has occurred. The final step in the JRC Trees-3 framework includes a visual validation process by experts, and therefore has an integrated validation program for processing. This is shown to be up to 90% accurate. The Trees-3 is also equipped to scale up the sub-national baselines in a consistent manner, and thus would contribute towards a transparent and internationally comparable baseline methodology. It does however, only cover tropical (humid) areas and within these, only forest land cover. Thus, it lacks a continuous cover for overall terrestrial land degradation.Another promising JRC land cover dataset is a phenology 5 -based land cover classification that can use multiple remote sensing products, including Landsat 8 (30m), MODIS (250m) and5 Phenology is the study of periodic changes, in this case in vegetation, influenced by e.g. climatic cycles and interannual changes of for instance leaves on trees. This can differ much between seasons and in some cases cause wrongful classification of the land cover (http://forobs.jrc.ec.europa.eu/products/software/).now also Sentinel 2 imagery (10m resolution) (http://forobs.jrc.ec.europa.eu/trees3/). This method has the same geographical structure and validation process as TREES-3 and exhibits equally high accuracy in tested areas (between 82% and 90%). It offers a few more land categories than TREES-3, shrub, grassland and sparse vegetation, but no land uses (e.g. cropland). The idea behind the phenology based land cover mapping is to capture differences in vegetation over the seasonal changes that might occur, and thus give a more accurate picture of the vegetation, than one time imagery. This method has been applied to Landsat 8 imagery and thus, only provides data going back to 2013, but is compatible with older products from Landsat imagery for backdating (Simonetti et al. 2013). The product is still being developed and tested and only a few areas have been mapped to date. Furthermore, the script is available for Google Earth Engine (a data archive and cloud computing platform), which is free but has a selective membership process through application.Terra-I is a fully automated near-real time observation system for monitoring deforestation activities, developed primarily for the humid tropics (www.terra-i.org/). Terra-I has finer spatial resolution than ESA CCI-LC, at 250 m at 16 day intervals, as well as the ability to discern between natural and human induced land degradation, which could prove important in determining land degradation drivers 6 . The model consists of a forecasting model that predicts future normalized difference vegetation index (NDVI) values 7 , based on historic greenness (MODIS NDVI time-series), and observed or estimated meteorological and climate fluctuations and thus estimates the probability of change attributed to human disturbances (Reymondin et al. 2013). This method has not been applied for assessment of land degradation as such, other than deforestation, and is as such not equipped to detect degradation in non-forested systems such as grassland or agricultural fields.The LandPKS currently consists of two smartphone apps -LandInfo and LandCover -for data collection (http://landpotential.org/index.html). The main aim of LandPKS is to use mobile technologies and cloud computing to crowd-source knowledge and information, expanding the concept of Ecological Knowledge (Herrick et al., 2013). The LandPKS has been applied for assessing soil texture and land cover, with case studies in Namibia (not in Otjozondjupa), Kenya, and Ethiopia. The method has not yet been applied to assess land degradation and is still under development. Thus, this system would have to be developed further, and may in the future offer a low-cost rapid assessment of LUC in LDN assessments.The LDSF (Vågen et al., 2013a) was designed for landscape-level assessments of land degradation (http://landscapeportal.org). It includes soil carbon dynamics and stocks, land cover change (vegetation cover and floristic composition), land use, soil health, hydrological properties, biodiversity, soil erosion and compaction. It uses \"sentinel sites\" designed to provide accurate baseline data and monitoring of land health. By combining field measurement protocols that are systematic with remote sensing data from a range of different platforms (e.g. MODIS, Landsat, RapidEye and Sentinel-2), the LDSF is being used for identification of land degradation hotspots and soil mapping at both regional and local scales.The LDSF has been applied in more than 35 countries in the tropics for baseline assessments of land degradation at multiple spatial scales, as well as for local assessments of soil health and land degradation with spatial resolutions ranging from 5 m to 30 m (Vågen et al., 2013b;Winowiecki et al., 2015) and at continental scale with a spatial resolution of 500 m (Vågen et al. 2016). The methodology has four main components: 1) A spatially stratified, hierarchical, field sampling design using 10x10 km sentinel sites; 2) Use of soil infrared (IR) spectroscopy for prediction of soil properties (for Indicator 3) 3) Use of remote sensing and ensemble learning methods for mapping of land degradation and land health; 4) Remote sensing for mapping of land cover and land use change. LDSF uses a combination of Landsat and MODIS derived data to determine the land cover, and thus has the same time series as these products.The following table includes a brief overview of the advantages and disadvantages of each method as mentioned in section 2,2 Land productivity is a measure of above ground net primary productivity (NPP), and is defined as the difference between the total photosynthesis and the total plant respiration in an ecosystem, or as the total new organic matter produced during a specified interval (Clark et al. 2001). It is reported in tons of dry matter per hectare per year (tDM/ha/year).Primary productivity of plants shows distinct dynamics over different temporal scales; daily variability due to the position of the sun, intra-annual variability due to seasonal effects and inter-annual variability due to changes in climate, or changes in management and land use. In order to detect degradation from temporal series of NPP, it is therefore important to filter out the effects of the vegetation's natural dynamics, that is specific for the type of ecosystem in question (Dutrieux et al., 2016;Jacquin et al., 2010).The LDN TSP (UNCCD 2016a) guides land productivity to be disaggregated by type of land cover it occurs in and productivity is therefore a proxy for management, land use intensity, and potentially degradation. If organic matter is extracted faster than it is produced NPP will decrease and is an indication that the ecosystem is being degraded (Haberl et al. 2001).Proxies for NPP, such as the much used Normalized Difference Vegetation Index (NDVI) (Rouse et al., 1974) from MODIS, basically account for the quantity of the standing biomass at a given time. Although biomass and productivity are closely related in some systems (Lohbeck et al., 2015), they can differ widely when looking across land uses and ecosystem types. For instance, a challenge with NDVI, specific to rangelands with bush encroachment, is that productivity will likely increase.In such a scenario, an increase in NDVI means that land degradation is occurring, where normally an increase of productivity would indicate the opposite. Similarly, intensive monocropping systems with fertilizer application could also produce a false positive, i.e. an increase in productivity that is not associated with a decrease of land degradation. In this case, fertilizer-use can mask the real state of the land under production (UNCCD 2015c). These scenarios need to be evaluated in the context of the region of the LDN baseline assessment (UNCCD 2016a). Also, there are discussions about whether NDVI tends to saturate when applied over densely vegetated areas (Huete et al., 1999), which is a concern when using NDVI to estimate NPP in forested regions, or when applying it to forest degradation (Kennedy et al. 2010).NDVI has also been suggested as a simple proxy for overall land degradation in areas where precipitation is not the main driver of vegetation dynamics, e.g. in humid tropics (Yengoh et al., 2014). However, the relationship of NDVI and -for example -soil erosion, which is a major land degradation process, is very weak (figure 2.3).Given some of the challenges with NDVI-derived products mentioned above, there are some commonly used vegetation indices that can be used as an alternative or an addition to traditional NDVI products. In the following section, firstly the JRC Land Productivity Dynamics (LPD) -default Tier 1 option for Land productivity as defined by UNCCD -is presented, and then three commonly used derivative products are discussed for their added value at national and/or subregional level: Enhanced Vegetation Index (EVI), Soil-Adjusted Total Vegetation Index (SATVI), and The Normalized Cumulative RUE Differences (CRD) index. These could potentially improve the existing NDVI based productivity estimates in certain contexts, either by themselves or as in complementation to NDVI.The JRC Land Productivity Dynamics (LPD) data has been proposed as a default dataset by UNCCD (2016a) when countries do not have better alternatives available. This dataset is based on a 15-year time-series of NDVI observations, and as such already includes the trends. It is produced at a spatial resolution of 1 km and data are classified into five productivity classes depending on the state of the system (see Table 2.3). The NDVI is adjusted for seasonality and phenology, as mentioned above, in an analysis of long-term changes (29 years, using NOAA GIMMS 3G) and current (5 year SPOT VEGETATION) efficiency levels of vegetative above ground biomass was combined into land productivity dynamics (Cherlet et al. 2013). A global map of LPD was furthermore derived by using 15 year SPOT VEGETATION 1999-2013. Increasing productivity IncreaseThe JRC dataset's 1 km resolution is unlikely to be of appropriate scale to reflect human activities at a sub-national scale (Ban et al., 2015), especially in small scale landscape mosaics. UNCCD (2016a) suggests using the above classification to determine the degree of degradation.The method relies on remote sensing measures of productivity trends of above ground biomass, such as NDVI, for different land uses, e.g. forest or agriculture.The Enhanced Vegetation Index (EVI) was proposed by the MODIS Land Discipline Group (Huete et al., 1999) as an improvement over NDVI, as it takes more information into account about vegetation and canopy structure, and is more reliable in areas that has a high biomass, where NDVI has been shown to saturate. EVI uses the same satellite imagery as MODIS NDVI and is thus also available at 250m resolution. It includes coefficients to reduce the influences of soil (i.e. light reflectance under the plants) and atmospheric conditions (i.e. light reflectance above the plants, e.g. cloud cover) on the VI 9 : The Soil-Adjusted Total Vegetation Index (SATVI) from Landsat 5 TM 10 was developed to specifically measure above ground biomass in arid vegetation, by including both green and senescent -dry -vegetation for rangeland monitoring (Marsett et al. 2006). Thus, the SATVI is more sensitive to dryland vegetation that has a weak signal of chlorophyll compared to other VI indices, as e.g. NDVI (Qi et al. 2002, Marsett et al. 2006) 11 :10 Bands 3, 5, and 711 Where ρ is the reflectance values of the different bands of the sensor: band 5 (short-wave infrared), 3 (red) and 7 (mid-infrared band).However, SATVI has been shown to falsely suggest vegetation in barren and rocky areas and such areas should be masked out before processing. Furthermore, it is limited to satellites using short-wave sensors, such as Landsat, which gives a minimum resolution of 30m (Marsett et al. 2006).The Normalized Cumulative RUE Differences (CRD) index uses Rain-Use Efficiency (RUE; Le Houerou 1984) to normalize MODIS NDVI and decouple the rainfall variability from productivity (Landmann and Dubovyk 2014). This separates human-induced productivity changes from climatic variations. CRD is thought to be an indicator for productivity decline in dryland areas, where vegetation dynamics are strongly linked to precipitation (Yengoh et al. 2014). However, there have been quite a few reported inaccuracies with this method, especially when modelling over short time series in high intensity agriculture systems, which challenge the use of CRD as a stand-alone indicator (ibid). Nevertheless, CRD has been shown to have an accuracy of 68% or higher in detecting changes in vegetation productivity depending on land cover type in drylands (Landmann and Dubovyk 2014).The following table includes a brief overview of the advantages and disadvantages of each method or datasets as mentioned in section 2.3. Soils that are losing organic carbon are experiencing degradation and SOC is thereby a key indicator of soil health. The organic carbon also plays an important role in the biogeochemical cycles that can restore soil health. Soil carbon sequestration is therefore recognized as an important strategy for both, climate change mitigation as well as adaptation.Indicator 3 reports on organic carbon stock above (biomass and leaf litter) and below ground (soil), however since above ground organic carbon is already to some extent reported through the UNFCCC, and there is no operational total terrestrial carbon estimation methodology to date, this baseline assessment will concentrate on Soil Organic Carbon (SOC). Furthermore, SOC has so far mostly relied on modeled trends based on LUCs (LDN- MN 2015, IPCC 2006). SOC is to be reported as tons of carbon per hectare (t/ha C). In the following sections, we review some of the existing methods that are available for a specific country or context to report and monitor SOC stocks, and we outline important considerations when assessing SOC.There are a number of existing methodologies and datasets available to measure soil organic carbon (Aynekulu et al., 2011;Ellis and Larsen, 2008;McBratney et al., 2006;Stockmann et al., 2013 among others). They differ in the sampling framework used, field sampling methods and observations, laboratory analytical methods, uncertainty and change detection, and costs. Most important, however, is making sure the sampling framework and associated measurements comply with the objectives of the study at the appropriate scale. Furthermore, one should carefully match the type of analysis with the appropriate sampling strategy. In fact this is a continually debated subject in the soil science community (Brus and de Gruijter, 1997;Heuvelink and Webster, 2001).Two widely used analytical methods to measure soil carbon concentration are the Walkley-Black (wet chemical oxidation) procedure (Walkley and Black, 1934) and dry combustion. Dry combustion is generally the recommended reference test for soil carbon as the Walkley-Black procedure only recovers about 75% of the SOC in the soil sample (Bhattacharyya et al., 2015). The latter method is, however, recommended when working in low SOC soils (less than 2%) (Rowell and Coetzee 2003).Soil infrared spectroscopy (IR) is another (emerging) technology that makes large area sampling and analysis of SOC feasible (Brown, 2007;Brown et al., 2006;Shepherd and Walsh, 2002;Vågen et al., 2006). The use of IR, and in particular mid-infrared (MIR) spectroscopy, produces consistent (and reproducible) predictions of SOC and allows for increased sample densities in order to capture the high spatial variability of SOC across landscapes (Terhoeven-Urselmans et al., 2010;Vågen et al., 2016). Recent reviews show a strong increase in the use of both NIR and MIR spectroscopy for soil analysis (Bellon-Maurel and McBratney, 2011;Stenberg et al., 2010;Viscarra Rossel et al., 2011). The use of IR data can be integrated with geospatial statistics and remote sensing for estimation of SOC concentrations and stocks at scales that range from local (within farm) predictions to continental assessments (Vågen et al., 2012, 2013b, 2016, Winowiecki et al., 2015, 2016). Another major advantage of IR for soil analysis is the capacity to predict many soil properties simultaneously from a single spectrum (Stenberg et al., 2010), which further reduces the total analytical costs of soil analyses. When combined with the high throughput achievable when using IR for soil analysis, inventories of SOC stocks at project level or larger geographical extents become feasible.Methods for mapping of SOC concentrations and stocks at different spatial scales are under rapid development, with significant progress being made based on the systematic collection of data on SOC stocks, combined with remote sensing and novel approaches for statistical modeling, such as machine learning algorithms. Previous estimates of the spatial distribution of SOC were generally based on available soil maps, such as the Digital Soil Map of the World (DSMW) and later the HWSD (Batjes, 1996(Batjes, , 2004;;Henry et al., 2009). These maps were produced at a very coarse spatial resolution, with large uncertainties, and were at best able to give rough estimates SOC stocks at continental scales. More recently, SOC has been mapped based on remote sensing, including air-borne light detection and ranging (LiDAR) (Asner et al., 2012), and space-borne Landsat (Vågen et al., 2013b), Quickbird (Vågen et al., 2012) and MODIS (Hengl et al., 2014;Vågen et al., 2016). Combining the use of cumulative soil mass to calculate SOC stocks with IR and remote sensing has been shown to have potential for assessing the spatial distribution of SOC stocks in landscapes (Winowiecki et al., 2016) by being both cost-effective, logistically efficient, and yielding high levels of accuracy.The default option set by UNCCD (2016a) to measure soil organic carbon is the SoilGrids database from the International Soil Reference and Information Centre (ISRIC) (http://soilgrids.org). In terms of the indicators relevant to LDN processes, the SoilGrids database provides predictions on soil properties and classes at a resolution of 250m (see figure 2.5). The SoilGrids system is freely available and can be used by countries that don't have more accurate national or sub-national data available. ISRICs main objective is to provide the international community with information on global soil resources, focusing on soil data and soil mapping, as well as the application of soil data.This means that the soil maps are based on the best global fit and might need field verification on a national or subnational scale to increase accuracy. However, SoilGrids is based on a continuously increasing number of soil profile descriptions (currently around 150.000) and covariates so that accuracy is increasing rapidly. Besides providing baseline maps of soil organic carbon, the mapping framework is built to accommodate shared soil data from different sources. Existing and newly gathered soil data (including soil spectroscopy data) for an area of interest can be uploaded -e.g. through the SoilInfo App (http://soilinfo.isric.org)to improve the accuracy of predictions.Soil organic carbon is predicted for six different depths, allowing for the calculation of carbon stocks, which can be used as approximations for baseline values for carbon monitoring. These baseline maps can also be used for optimization of sampling strategies for future carbon monitoring.For Africa, the 250 m soil property maps used a combination of legacy soil data from the Africa Soil Profiles databases and soil data collected as part of the Africa Soil Information Service (AfSIS) project. The SoilGrids framework can also be used as a tool for monitoring and evaluation. The combination of a) an automated mapping system and b) a mechanism for crowdsourcing makes SoilGrids a tool for monitoring changes in soil organic carbon. Regular model runs will use the new sets of observations or measurements contributed, and automatically produce new maps for the area of interest.The following table includes a brief overview of the advantages and disadvantages of each method or datasets as mentioned in section 2,4. This chapter focuses on the process of evaluating the methods that have been presented in chapter 2, plus any additional methods as well as tier 2 and 3 data available or being developed in Namibia and Costa Rica and identified during local workshops. Furthermore it describes the evaluation of additional indicators beyond the UNCCD indicators Land cover, NPP, and SOC. The overall evaluation process consisted in selecting and weighting different criteria, ranking the available methods, and developing a plan for the implementation of baseline production.Multiple sources of combined data are likely to be most precise, as was the conclusion from earlier land degradation assessments (LADA/GLADIS 2009, Sommer et al. 2010, Reed et al. 2011). This is especially relevant if assessments must capture changes over time in at fine scale with heterogeneous land uses, compared to changes at large spatial scales.While general criteria for LDN methods are presented in Box 1 above, national partners in Namibia and Costa Rica stressed that the following three criteria, in particular, were crucial for a baseline assessment. The selected method(s) must be:1. cost-effective, 2. appropriate for the available national capacity and be repeatable independently by local partners, and 3. have value for other regional and national projects. This specific emphasis does not necessarily imply that partners are less interested in methods that offer the highest resolution or accuracy. However, final decisions on the methodologies selected depend to a great extent on data requirements for local initiatives and capacities to implement the methods independently.For instance, in Namibia, the pilot area is much larger and local capacities are being developed, partners are less interested in the latest techniques, if this means that the method is not costeffective or that the methods have to be implemented by non-Namibian organizations. Contrarily, in Costa Rica the area is much smaller and there is a need for high resolution data. Here national institutions and universities have varying capacities, and thus partners were more interested in robust methodologies, that generate highly detailed information needed for local planning and local/national implementation.Many countries have other commitments to the different UN conventions. It is therefore not desirable to increase the burden on national governments for reporting. One way to lessen the burden and be more cost-effective is to share datasets and monitoring tasks, for example on land cover and land use change. It is also increasingly more important that methods meet the available capacity and can be built to implement the methods and monitoring system independently.Partners in Namibia and Costa Rica also emphasized that the LDN baselines needed to support ongoing development and sustainable land management plans. Where methods can support such plans, they will find greater buy-in and political support. This is especially important when the baseline is complete and targets are set for reaching land degradation neutrality. For example, degraded agricultural lands may already have targets related to NPP and SOC and the LDN targets should not conflict existing targets. Similarly, areas may already have land cover and land use change targets such as reforestation of areas that were previously deforested. Therefore, selecting a baseline method needs to be informed by ongoing national and regional (planning) processes and sustainable development plans.One of the difficult tasks with multi-criteria tradeoff analyses is setting weights for different criteria. For example, it is not a trivial task to compare cost-effectiveness and national capacity. Perhaps one method provides an opportunity to train national staff in a desired methodology but it does not provide the proper time-frame (10 years) to establish a trend for LDN. Weighing or ranking different criteria with a large group of workshop participants who have different perspectives can become problematic. Even the process of who is invited to the workshop and gets to decide, influences the process. Another challenge is that facilitators and decision makers often have to work with incomplete information. For example, not all the cost of implementing different methods were available at the time of the workshops, which was a challenge given that cost-effectiveness was one of the most important criteria.During the workshops, it was therefore important to have a transparent process and recognize the limitations of selecting methods with incomplete information. There was deliberately extra time built-in for debate and discussion, and time was taken during each step of the process to make sure issues were clearly disseminated and understood, and thus that there was consensus to move forward. Overall, the selection process followed a three-step approach:1. Overview of available information on methods, including national approaches, and reaching agreement on additional indicators where necessary 2. Weighting of selection criteria by LDN indicator 3. Completion of selection matrix to select one method for each indicator During each workshop, presentations were given by experts on the different methods for each indicator (see chapter 2). Workshop participants were given ample time to ask detailed questions to make sure there is clear understanding of the pros and cons of the different methods. Local experts were also invited to give presentations on approaches used at the national level. The tables presented in chapter 2 at the end of each section were very useful to stimulate discussion. Two approaches were used for weighting and scoring (1 to 5, with 1 being unsuitable and 5 being highly suitable) the different options. In Namibia, participants were divided in groups and each group discussed how the criteria should be weighted, and what score should be given for all three LDN indicators. The scores were then combined to determine the approach for each indicator. In Costa Rica, the workshop participant group was larger and there was more expertise in specific topics so that the groups were divided according to the LDN indicators. In each sub group, participants were asked to weigh the criteria and then assign scores to the different methods. In both cases, the groups had to agree to give one score collectively. This requires that everyone is engaged and this approach promotes discussion. Having a facilitator in each group helped to guide the discussion and allow all members of the group to be heard. Alternatively, we could have asked each participant to give a score which allows all participants to participate fully and does not allow a single person to dominate the discussion.The final step could not always be completed given the lack of critical information as described above. In both Costa Rica and Namibia, additional information was collected after the workshops ended. In Costa Rica, participants selected four methods for the three LDN indicators plus erosion risk. They also expressed a desire for more training to understand some of the methods better and be able to make more informed decisions at a later stage. In Namibia, participants narrowed it down to two approaches and requested that the budgets were worked out first so that the final decision could be taken by the Ministry of Environment and Tourism, which is responsible for executing the LDN agenda in Namibia. Before we present the final selections, an overview of each region is given below.The Otjozondjupa Region (figure 3.1) is situated northeast of the capital of Windhoek and spans 105,460 km 2 and a low population of approximately 144.000 people (0.73 persons/km 2 ) (Namibia Statistics Agency 2011). The region is predominately characterized by grassland and sparsely vegetated shrubland, and scattered small areas of closed canopy forest. The land tenure is predominantly privatized, except for the community lands in northeast districts. Land use is mostly rangeland cattle farming, much of it being intensive commercial cattle farming, grain production, and a large proportion of smallholder subsistence agriculture mainly in the communal lands (King et al. 2011, Gilolmo andLobo 2016). Namibia is naturally the most arid country in sub-Saharan Africa, and prolonged droughts are well-known occurrences, which is projected to increase and become more unpredictable in the future (Ziedler 2010).Bush encroachment represents a great threat to the livelihoods of the (e.g. pastoralist) communities living in dryland ecosystems (e.g. Angassa and Oba, 2008). It is described as the increase in biomass and abundance of woody species and the suppression of perennial grasses and herbs (Ward, 2005) leading to dense thickets often composed of thorny and/or unpalatable bushes. It often occurs as a result of land degradation in African drylands, for example due to overgrazing or changes in fire regimes (Zimmermann et al., 2008). Once established, invasive woody species can also be a major driver of land degradation due to the suppression of perennial grasses and reduced ground cover as a result (Joubert et al., 2008) and soil nutrient depletion (Klintenberg and Seely, 2004;Moleele and Perkins, 1998;Oldeland et al., 2010;Rocha et al., 2015).In Africa, certain species of the genus Acacia are known encroachers. In Namibia, grasslands are often encroached by Acacia mellifera and Acacia reficiens, often occurring together (Joubert et al., 2008;Zimmermann et al., 2008). While these are native, encroaching species invade land by being very efficient in utilizing available resources (nutrients, water, light, energy), and have traits that allow them to quickly take up these resources making them unavailable for other plants (Funk and Vitousek, 2007).The negative consequences of bush encroachment are widespread and include: adverse effects on native species (Meik et al., 2002;Spottiswoode, 2009), diminishing agricultural production, rangeland degradation (Angassa, 2005), watershed quality (Huxman et al., 2005), increasing erosion (Grover and Musick, 1990;Vågen and Winowiecki, 2014) and loss of ecosystem carbon (Jackson et al., 2002) and loss of aboveground biodiversity. (Jackson et al., 2002) reported higher SOC in encroached areas in dry areas and a decrease in SOC in wetter regions.The deeper and more expansive root systems of many woody vegetation types, compared to herbaceous plants, means that they have access to soil water from deeper soil layers, which in turn means that they tend to have longer seasonal periods of water extraction and can thereby reduce soil water content consistently throughout the year (Kemp, 1983). In addition, their greater leaf area will increase water loss by transpiration and increase soil evaporation through the exposure of the soil (Huxman et al., 2005). As a consequence, encroachment has been shown to directly decrease streamflow in some cases (Cleverly et al., 1997).While there are limited data available on degradation currently, Namibia is concluding a number of action-plans to combat environmental degradation and integrate management of all the regions as well as land tenure reforms, and thus centralizing national data might become a priority in the near future (King et al. 2011). Globally available datasets show an increase of NDVI in grassland regions which is most likely due to bush encroachment (LDN Country Report 2015, Gilolmo and Lobo 2016). Bush encroachment is not always a direct consequence of land use change however. Drivers of bush encroachment are much debated (Ward, 2005) and likely include a combination of different factors including grazing intensity, fire management, and climate change. Unless extremely severe, it is unlikely that the three standard LDN indicators recommended by UNCCD can detect bush encroachment.To our best knowledge there are no existing tier 2 or 3 land cover maps for Otjozondjupa Region that could improve on the default ESA CCI land cover maps. However, Namibia is currently developing the Integrated Regional Land Use Plan (IRLUP) based on participatory land use planning which is scheduled to be completed in 2016. The plan will include georeferenced land use maps for each region for both present and future land uses.Changes in productivity presume that healthy land exhibits a high productivity while degradation reduces the net primary productivity, but that is a matter of context as outlined above. For Namibia, bush encroaching vegetation increases (aboveground) biomass and productivity compared to the grassland it encroaches. Therefore, applying straight forward Tier 1 productivity maps as an indicator, may erroneously classify degrading encroached areas as areas under recovery. Bush encroachment in drylands can be difficult to detect with some of the most commonly used VIs, such as NDVI and CRD, since many of the new species found in these areas have a relatively weak signal in terms of chlorophyll. Other types of VIs have been explored as alternatives, including the EVI and SATVI (Marsett et al., 2006;Qi et al., 2002) as they tend to be more sensitive to canopy structure and dryland vegetation cover (i.e. senescent vegetation).There is an existing program in Namibia that monitors land productivity trends based on MODIS data. The Rangeland Monitoring Project (http://www.namibiarangelands.com/) publishes mean monthly NDVI, precipitation, and vegetation condition maps on their website. The project is also developing new methods to map bush encroachment that will become available in 2017.In addition to Figure 2.5 above (SoilGrids250), Figure3.2 shows a soil carbon stock map based on the LDSF framework. Existing studies of SOC for Otjozondjupa and surrounding areas have reported values ranging from about 6.2 g C kg -1 to 11.5 g kg -1 in the topsoil (0-20 cm depth) in rangeland soils. Somewhat higher SOC concentrations can be expected in dense bushlands or dry forests. based on the LDSF (see Vågen et al., 2016) As described in chapter 2, the methodologies that were used to make these maps are based on soil profile data collected in sites across the world (so called sentinel sites), and thus have a global best fit, not a national or regional best fit. There are no tier 2 or 3 data available in Namibia to test the in situ accuracy of either map, although these methodologies have a published accuracy based on studies done elsewhere. One way forward would be to improve on these two methods by collecting local data to validate the models.Biodiversity and functional properties (e.g. time of leaf emergence) of the vegetation are important indicators of land degradation, and using these as additional indicators will be helpful especially for detecting bush encroachment as this may prove to be challenging using the current tier 1 indicator. Remote sensing has been used for mapping of woody species diversity (Innes and Koch, 1998;Rocchini et al., 2015) and can be applied in the assessment of land degradation. Such additional indicator may help in the detection of bush encroachment as species composition and biodiversity change in bush encroached areas. Some remote sensing techniques can also distinguish between the leaf phenology of the two types of Acacia prevalent in bush encroachment areas in Namibia (Oldeland et al., 2010): A. mellifera, which has leaf flushing in September and A. reficiens, which has leaf flushing in December. The JRC Phenology land cover data could potentially detect the differences in phenology and thus bush encroachment at 30 m resolution. Furthermore, dryland grasses have different metabolic pathways in the photosynthesis than bushes and trees, called the C4-pathway. Recent techniques can detect this and use C3/C4 carbon isotope ratio in soil carbon and map this using remote sensing. This can be used to assess bush encroachment, because the relative abundance of C3 carbon will be higher in soils under woody vegetation, as compared to soils under tropical grasses that have a C4 photosynthetic pathway.The Rio Jesus Maria watershed (352 km 2 ) in Costa Rica is very different from the Otjozondjupa region in Namibia and thus other issues arise when developing a baseline. Rio Jesus Maria watershed is situated along Costa Rica's Pacific coast (Figure 3.3) and has lost most of its natural vegetation cover due to deforestation and agricultural expansion. Currently the majority of the watershed consists of grasslands for extensive cattle ranching and secondary forests (Centro Agronómico Tropical de Investigación y Enseñanza (CATIE), 2011). Reduced vegetation cover, poor infrastructure, and extensive cattle ranching have led to wide scale soil degradation and erosion and the concomitant widespread loss and redistribution of soil and nutrients in the watershed, degradation of soil structure, water pollution and downstream floods.Studies show that local communities, especially the ones living downstream, are affected by lack of fresh water supply, failing crop yields during floods, ultimately increasing food insecurity and poverty in the region (Centro Agronómico Tropical de Investigación y Enseñanza (CATIE), 2011). Floods sometimes also inundate populated areas resulting in damage to infrastructure and properties.The complex topography of the Rio Jesus Maria watershed, and its relatively small size, means that assessments of land degradation need to be made at fine spatial resolution to capture the level of degradation properly. This will present a challenge for most tier 1 remote sensing products publicly available, as their spatial resolution is most often too coarse to capture smallscale dynamics. Furthermore, the use of remote sensing products can be challenging as there is often extensive cloud cover in the area. However, Costa Rica has a suite of tier 2 data developed and available.Land cover is important for detecting deforestation which is the primary cause of land degradation in Rio Jesús Maria watershed. However, the resolution of land cover change detection plays an extremely important role in being able to detect land degradation. In addition to changes in land cover from forest to non-forest, combinations of land use with soil type, topography, and management (practices / intensity) also drive land degradation in this geographical context (Hoyos, 2005). As a result, the direct relationship between land cover change and watershed degradation may not always be accurate (Ponette-González et al., 2015).The coarse resolution of a tier 1 dataset describing forest loss/gain, does not match the trends estimated by national institutions as part of the national programs for monitoring forest cover (MINAE 2015). As the national initiatives' tier 2 datasets use finer resolution and are linked to the national programs for payment for ecosystem services and REDD, existing data such as those produced as part of various initiatives to map forest cover in Costa Rica may be very useful for assessing land cover.Costa Rica developed various policy reforms and incentives in the 1990s to stop deforestation and forest degradation, and favor reforestation in areas where forest cover was lost. The main national mechanisms for support of reforestation are the national forestry development plan and the national program for payment of ecosystem services managed by the National Fund for Forest Financing (FONAFIFO) of Costa Rica. In order to track progress linked to these national policies and incentives, FONAFIFO and the National System of Conservation Areas (SINAC) have worked to develop forest cover maps for 2000, 2005, 2010 with a resolution of 30 x 30 m. These maps were derived from Landsat and SPOT images and focus only on two types of land cover: forest and non-forest.In 2013 SINAC updated the national forest cover map using RapidEye Images (from December 2011 until July 2012) to generate the first forest cover map at resolution of 5m. This map identifies eight categories of forest, from pristine mature forest, secondary forest, deciduous forest, palm forest, silvopastures, forestry plantations, mangroves and paramo. Other lands uses such as annual and perennial crops, wetlands, water bodies, bare soil, sand and urban infrastructure are grouped in one category of \"non-forest\". This detailed forest map was the basis for the stratification and location of sample plots for the 2013 National Forest Inventory (SINAC 2015) (see Figure 3.4).In order to cover a multitude of reporting methodologies, Costa Rica is developing a national system for monitoring land use change dynamics. This system should provide information on land cover, land use change for forest and other important ecosystems, and will be the cornerstone for reporting on national targets to reduce greenhouse gas emissions (linked to REDD and Nationally Appropriate Mitigation Actions [NAMA]). A group of experts is currently working on the development of this system, particularly in the development of a unique land classification system for the country. The monitoring methodologies have not been defined yet and could therefore be streamlined with LDN reporting needs. Costa Rica is also in the final period of consultation of its National REDD+ Strategy, and has submitted its proposed national Forest Reference Emission Level and Forest Reference Level (FREL/FRL) to the UNFCCC for a technical assessment. Although Costa Rica included all REDD+ activities in its national REDD+ strategy, only emission reductions from deforestation and enhancement of forest carbon stocks have been included in the FREL/FRL. FREL results are based on a temporal analysis of land use change for the period 1986-2013, using Landsat images. The classes recognized in these maps are: forest, annual crops, permanent crops, grasslands, settlements, wetlands, paramos and natural and artificial base soil (MINAE 2016).The land productivity indicator involved vivid debates during the workshop. This was due to several factors. First, it was acknowledged that many different institutions had satellite data from different sources with different resolutions, and without a clear overview of the content and availability of the national repositories, it was difficult to make this decision. During the post-workshop period, it was proposed that this would be a separate consultancy, to do a complete review of data and capacities within Costa Rican institutions and to produce a land productivity baseline, which would be appropriate for both the small watershed as well as the national scales.Since there is a strong emphasis on monitoring forest cover and deforestation, and given the relationship between forest cover and land productivity, efforts to produce these indicator baselines may be combined. For example, there is a possibility to use the newly developed FREL/FRL for reporting to the UNFCCC, to establish forest change, as an indicator for land productivity. In combination with other data, or as a stand-alone, these data might hold potential for improving spatial resolution, have long time series, and thus improve the indicator for trends in land productivity.One other possibility that takes advantage of the relationship between land productivity and deforestation is the Terra-I framework which was reviewed in section 2.2.5. Terra-I monitors deforestation in near real-time, based on changes in productivity, and is freely available. While it has better spatial resolution (250m) than JRC LPD (1km), the workshop participants regarded this as too coarse. We mention it here to highlight potential ways to combine future efforts in Costa Rica in completing the baselines for both land cover and NPP.Currently there are no completed maps of soil organic carbon for Costa Rica available from national initiatives. Various institutions are working on digital soil mapping as well as on collection and systematization of nationally available soil data. For example, the University of Costa Rica and the National Institute of Agricultural Technology (INTA) have been working with the Soil Information System for Latin America (SISLAC). SISLAC is a collaboration between FAO -Global Soil Partnership, CIAT, Catholic Relief Services (CRS), EMBRAPA (Brazilian Agricultural Research Corporation), and national institutions from 19 countries in Latin America. As part of SISLAC, Costa Rica received training in digital soil mapping and has started to produce draft SOC maps. At the time of the workshop, 500 soil profiles were available which has now tripled to 1500 soil profiles, mainly available for coastal areas which had been prioritized. In addition, the National Forest Inventory collected 280 soil samples from 1,000 m 2 in 2013, covering six different forest land uses (Figure 3.5) across the whole country. Some readily available SOC maps could be used as a SOC baseline map. For example, LDSF has a 500 m resolution map of SOC for Jesus Maria watershed (Figure 3.6). However, this resolution was considered too coarse by the national stakeholders who participated in the workshop. Others are also working on making global SOC maps available at finer resolution. ISRIC, for example, has made available a 250 m resolution product recently. In order to make meaningful assessments of SOC status and trends we suggest that these will need to be made at 30 m or finer resolution for this watershed.The currently available data may not be sufficient to produce a reliable estimate of SOC in the Rio Jesus Maria watershed because of scattered sampling plots that only cover forest land covers; with only few data points available in Jesus Maria. Therefore there is a need to increase the number of soil measurements to be able to develop digital soil maps for Jesus Maria. Also, while digital soil mapping training has started through the SISLAC collaboration, there is still an expressed need to further strengthen this through capacity building. (Vågen et al., 2016).A critical factor determining SOC trends in the Jesus Maria watershed is likely to be soil erosion, particularly along waterways and on steep slopes, as highlighted in the Community Development and Knowledge Management for the Satoyama Initiative 13 (COMDEKS). It is recommended as an indicator that should be considered, in addition to the three core indicators, when assessing land degradation in the watershed.National efforts to assess soil erosion in Costa Rica, currently focus on assessing the vulnerability of soil to be eroded using the PAP/RAC methodology. During a presentation by INTA at the workshop in San José, it was explained that this method does not quantify the amount of soil lost due to water or wind erosion, but it gives an index defining the grade of erosion risk/potential (Priority Actions Programme Regional Activity Centre -Split 1997). Soil vulnerability to erosion is based mostly on soil physical properties and land cover. INTA and the University of Costa Rica are tailoring the methodology to conditions in Costa Rica and are updating the soil map of Costa Rica to have a better application of PAP/RAC methods in areas where soil information is not complete. Part of these efforts include plans to develop a detailed soil sampling to characterize soil profiles and soil properties in particular areas of the country.The soil sampling methodologies used by INTA could also complement the efforts to collect new soil samples in Jesus Maria in relation to soil organic carbon.The Namibia workshop was conducted from February 9 to 11, 2016, in Windhoek. The workshop was organized in collaboration with the Ministry of Environment and Tourism (MET). One factor that was important in Namibia was the fact that very little data already existed for Otjozondjupa region. While the basic data to determine land productivity through net primary production is available at national level, there are no national baselines for bush encroachment or SOC (other than the ISRIC and LDSF datasets that are based on modeling techniques and do not yet include ground truth data). National land cover data are very coarse, and thus not of much relevance for Otjozondjupa. Furthermore, the severity of bush encroachment in Otjozondjupa and other areas of northern Namibia was critical during the methods selection process. It became obvious that extensive new field data had to be collected to map areas under bush encroachment and accurately map bush densities as well. The requirement to do extensive fieldwork influenced the selection of a methodology for the three remaining LDN indicators.Given that extensive areas will have to be surveyed to collect data on bush encroachment, it was a logical next step that other land cover data would be collected at the same time in order to be cost-effective and complete the land cover and SOC baseline maps. During method selection, the emphasis was thus placed on optimal and efficient field sampling design for collecting data for three indicators: land cover, soil organic carbon and bush encroachment. Another factor that influenced the selection of a method for SOC measurement was the desire to execute the different components of the work locally, for as much as feasible, and therefore give preference to techniques that are locally and nationally used already. While the LDSF method was desirable because it presented a complete package for different indicators, the methodology relies on the use of soil infrared spectroscopy which is currently not available in Namibia so it was not the preferred option by the participants.The final selected methods were thus:• Indicator 1 -Land Cover: ground truth data collection for a supervised classification of Landsat data at 30m resolution. • Indicator 2 -NPP: collaboration with local partners to produce a NDVI-derived baseline using the same Landsat data. • Indicator 3 -SOC: field data collection of soil profile data, analyzed locally at the Ministry of Forestry, and baseline produced using the ISRIC methodology.• Bush Encroachment: field data collection to produce a baseline with different gradients of bush encroachment (density) based on the methodology for Land Cover.Appendix A provides a summary of the activities and expected cost for implementing this approach.The Integrated Regional Land Use Plan (IRLUP) is an important way forward to address land degradation in Otjozondjupa. Any targets for achieving land degradation neutrality should be integrated into general land use planning. Another project of interest for the LDN study in Otjozondjupa Region is the GIZ De-Bushing Project (DBP). This project aims to quantify the extent of existing bush encroached area and determine its spatial distribution, as well as the possible economic utility of the encroaching biomass (e.g. for charcoal production). Furthermore, a proposal to establish a GIS database of the dynamics of bush encroachment (the BIS-GIS database) is pending a second round of feedback from the Namibian Statistics Agency (NSA). The outcome of this project could offer valuable information on methodology and extent of bush encroachment in the Otjozondjupa region for the LDN baseline procedures.The review of the BIS-GIS has shed light on a few considerations expressed by National Statistics Agency (NSA), which are of relevance to the LDN methodology. Firstly, it is important that a baseline assessment relies on quantitative and ground-truthed data, and that the methodology requires validity testing to determine the level of accuracy of the products. It is also important to use existing technology and infrastructure made available by various ministries (e.g. Department of Surveys and Mapping, DSM), to reduce uncertainties and replication of already derived information and adhere to existing policies and quality standards. Secondly, spatial, temporal, and methodological consistency of available remote sensing and vector data are an expressed concern of the NSA, as not all regions of Namibia are equally well-covered. This will most likely not affect the pilot area of Otjozondjupa much as there is consistent remote sensing data coverage in this area, but might be of concern to other regions of Namibia in a national baseline assessment framework. Given the strong capacity in Costa Rica, most of the selected methods were methods that are currently used nationally and that national experts are already familiar with. The final method selections were:• Indicator 1 -Land cover: Use the same methodology that is used to produce land cover maps for Costa Rica's REDD+ (Reduced Emissions from Deforestation and Forest Degradation) commitments to the UNFCCC. • Indicator 2-NPP: The approach is to first investigate which data are nationally available from different government offices. Then it will be necessary to agree on which of the indexes will be used for the baseline analysis of NPP and decide whether the national organizations or an external consultant will carry out analysis to complete the baseline. In Costa Rica, there are several planning processes on sub-national level that are important to address land degradation in general and for specific pilot areas, for example, municipal regulation plans, territorial rural development plans, management plan for protected areas, management or strategic plans for biological corridors, beside others.The following considerations expressed by different stakeholders in Costa Rica are of relevance to the national LDN process:First, a baseline assessment for LDN should rely on quantitative and ground-truthed data, be as accurate as possible using the existing data bases, technology and infrastructure available in the country to reduce uncertainties and replication of already derived information.Secondly, a baseline assessment for the proposed, relatively small, pilot area should be applicable on national level for a national baseline assessment.Thirdly, there are several ongoing projects of interest for the LDN process in Costa Rica.-The Sixth Operational Phase of the GEF Small Grants Programme in Costa Rica (2016-2018) aims at enabling community organizations to enhance livelihoods by restoring degraded forest and production landscapes for socio-ecological resilience in the watersheds Jesús Maria and Barranca. It supports, among other, adaptive landscape management plans and policies, reforestation and restoration campaigns. -The GIZ-Project \"Implementation of the National Biocorridor Programme (PNCB) within the context of Costa Rica's National Biodiversity Strategy\" (2014-2020) commissioned by the German Federal Ministry for the Environment, Nature Conservation, Building and Nuclear Safety (BMUB). This project supports, among other, the establishment of a baseline and monitoring system for biological corridors including in parts the same or similar indicators as proposed for LDN. Furthermore, the project facilitates planning processes for sustainable land management in biological corridors. In addition, advice for the establishment of financial mechanisms generates funding for implementing relevant SLM measures. -The \"NAMA support project: Low-carbon coffee Costa Rica\" (2016-2019), commissioned by the German Federal Ministry for the Environment, Nature Conservation, Building and Nuclear Safety (BMUB) and the UK Department for Business, Energy & Industrial Strategy (BEIS). This project supports the voluntary action geared to climate change mitigation, including efficient application of fertilisers, the use of shade trees and measuring, reporting and verification of CO2 reductions.The LDN Target Setting Programme of the UNCCD focuses preliminary on the national scale for land degradation assessments and LDN target setting. In order to support the implementation of a LDN approach, national assessments need to be complemented with more detailed sub-national baseline assessments. This report provides a review of the available data and methods for the three LDN indicators in the context of two pilot areas. These areas -Otjozondjupa region in Namibia and Rio Jesús Maria in Costa Rica -were identified with the respective partners participating in the national LDN processes, because both have degradation challenges that are relevant for many regions around the world. These challenges are important to address and strengthen national capacities and readiness to engage in mitigating land degradation.There are a number of existing and free global datasets that could offer information to the land degradation assessments at sub-national scale. For Indicator 1 Land Cover Change there are multiple land cover maps available, e.g. GL30, JRC Trees-3, Terra-I, and LDSF, which are viable options to complement the default option, the ESA CCI-LC dataset. These datasets and methods can be used as stand-alone or as supplements to national data. Indicator 2 Land Productivity data are also available at the global level, which serve to derive more accurate data (e.g. using EVI, SATVI or RUE). It is suggested to use NDVI derived products that are more sensitive to senescent vegetation in dryland areas, soil conditions, and that have atmospheric adjustments in areas with extensive cloud cover. Moreover the resolution of the freely available data is not suitable for supporting local planning in countries such as Costa Rica where there is great variability in biophysical conditions over small areas.The negative impacts of bush encroachment are widespread and include: diminishing agricultural production and rangeland degradation, decreasing watershed quality, and loss of aboveground biodiversity. While bush encroachment is considered the most severe form of land degradation in Namibia, it has confounding challenges for all three LDN indicators. This is because bush encroached lands usually show positive trends in net primary productivity and soil organic carbon, and they will reflect a land cover change from grassland and shrubland to bushland and forest, which is considered a positive change from the LDN perspective.Workshops were held in both Namibia and Costa Rica to discuss the process with local stakeholders. For LDN methods national partners stressed that the following three criteria were important to take into account. The selected methods should be:1. Cost-effective 2. Appropriate for the available national capacity and be repeatable independently by local partners 3. Have value for other regional and national projects Both focus areas have a number of ongoing projects and processes that can be used to share either reporting obligations or primary data collection, and building on these commonalities can offer a reduction in costs to the LDN baseline assessment and future monitoring efforts.In Otjozondjupa, Namibia, the main problem associated with land degradation in dry grassland is bush encroachment. There is no existing data on bush encroachment available, and therefore other options were explored further. Namibia is furthermore experiencing a lack of both existing national data, as well as a centralized data repository, and data that is available is not readily accessible. Namibia chose to opt for classification and ground-truthing of finer spatial resolution from globally available Landsat imagery and SoilGrids methodology, and extensive data collection to map distribution and densities of bush encroachment. The final selection was as follows:• Indicator 1 -Land cover: ground truth data collection for a supervised classification of Landsat data. • Indicator 2 -NPP: collaboration with local partners to produce a NDVI-derived baseline using the same Landsat data. • Indicator 3 -SOC: field data collection of soil profile data, analyzed locally at the Ministry of Forestry, and baseline produced using the ISRIC SoilGrids methodology. • Indicator 4 -Bush Encroachment: field data collection to produce a baseline with different gradients of bush encroachment (density) based on the methodology for Land Cover mapping.In Rio Jesús Maria watershed, Costa Rica, deforestation and subsequent watershed degradation has led to severe soil erosion. Costa Rica has a large collection of national data, and local experts therefore opted to expand on existing national data for the three indicators, as well as increase national capacity on soil mapping and erosion modeling. The final selection was as follows:• Indicator 1 -Land cover: Use the same methodology as used to produce land cover maps for Costa Rica's REDD+ commitments to the UNFCCC. • Indicator 2 -NPP: The approach taken is to first investigate which data are nationally available from different government offices and task one with producing an NDVI change analysis to complete this baseline. • Indicator 3 -SOC: Costa Rica has started work on SOC mapping under the SISLAC framework and decided to complete this work. In addition, workshop participants requested additional training in other Digital Soil Mapping techniques in order to do a more robust evaluation of all available techniques at a later time. • Indicator 4 -Erosion (existing erosion and erosion risk): Participants selected training in the RUSLE-3D technique to produce a map of current erosion to complement their modified PAP/CAR approach.Both countries have existing processes in place or starting up, which might be helpful to the LDN baseline assessments and reduce cost of primary data collection. In Namibia this is namely the IRLUP process, but also the GIZ De-Bushing Project and The Rangeland Monitoring Project, can assist in addressing the gaps in national data. For Costa Rica there are a number of ongoing national processes that will enable more extensive national data on land degradation, most importantly improvements of their reporting mechanisms to the UNFCCC and the engagement with SISLAC.While the pilot phase provided a national baseline based on globally available datasets, this review of other datasets and methods points to a need for higher resolution (primary) data in order to develop baselines for the two sub-national regions in Namibia and Costa Rica. Moreover, subnational baseline assessments may require additional field data collection depending on the characteristics of degradation occurring locally. In certain contexts, additional indicators beyond Land Cover, Land Productivity and Soil Organic Carbon, may be required to adequately assess land degradation at a subnational level.","tokenCount":"12080"} \ No newline at end of file diff --git a/data/part_6/0588902052.json b/data/part_6/0588902052.json new file mode 100644 index 0000000000000000000000000000000000000000..4b41ab97b6815d7d316387f997f029cfaf081ad1 --- /dev/null +++ b/data/part_6/0588902052.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"00861aa59fe78778a854503be9a528ba","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/b95cf107-2938-4a8b-abac-a1eb48ff8547/content","id":"-1372835823"},"keywords":["AFLP, DNA fingerprinting, genetic diversity, synthetic wheat AFLP, amplified fragment length polymorphism","CIMMYT, International Maize and Wheat Improvement Center","DNA, deoxyribonucleic Acid","PCA, principal component analysis","PIC, polymorphic information content","RFLP, restriction fragment length polymorphism","SSR, simple sequence repeat","UPGMA, unweighted pair-group method with arithmetic averages"],"sieverID":"27673e98-46d8-4dfa-8b71-75e52eb34504","pagecount":"12","content":"Genetic diversity among 14 drought tolerance (drought accessions) and 27 salinity tolerance (salinity accessions) related conventional and synthetic wheat (Triticum sp.) accessions containing different sources of the D genome was assessed using amplified fragment length polymorphism (AFLP). The wheat accessions were analyzed with 20 EcoRI/MseI primer combinations. Among 918 fragments scored, 368 were polymorphic across all 41 wheat accessions, 348 were polymorphic among the drought accessions and 310 were polymorphic among the salinity accessions. Similarity coefficients among all accessions based on Jaccard's coefficient ranged from 0.18 to 0.92 with an average of 0.53 ± 0.01; among drought accessions, from 0.16 to 0.79 with an average of 0.43 ± 0.02; and among salinity accessions, from 0.16 to 0.92 with an average of 0.57 ± 0.01. Polymorphic information content (PIC) among all accessions ranged from 0.05 to 0.50 with an average PIC of 0.30 ± 0.01; among drought accessions, from 0.13 to 0.50 with an average PIC of 0.37 ± 0.01; and among salinity accessions, from 0.07 to 0.50 with an average PIC of 0.29 ± 0.01. Cluster and principal component analysis showed distinct groups of accessions both within drought and salinity entries. These accessions possess a substantial amount of genetic diversity and would be very valuable materials for breeding wheat with drought and salinity tolerance.Das, M. K., Bai, H-H. et Mujeeb-Kazi, A. 2007. Diversité génétique des variétés classiques et synthétiques de blé tolérant la sécheresse et la salinité selon la technique AFLP. Can. J. Plant Sci. 87: 691-702. Les auteurs ont évalué la diversité génétique d'obtentions de blé (Triticum sp.) classiques et synthétiques tolérant la sécheresse (n = 14) ou la salinité (n = 27) et renfermant différentes sources du génome D par la technique du polymorphisme amplifié de la longueur des fragments (AFLP). Les obtentions de blé ont été analysées grâce aux combinaisons d'amorces 20 EcoRI/MseI. Sur les 918 fragments évalués, 368 étaient polymorphes chez les 41 obtentions, 348 étaient polymorphes chez les obtentions tolérant la sécheresse et 310 étaient polymorphes chez celles tolérant la salinité. Le coefficient de similarité reposant sur le coefficient de Jaccard variait de 0,18 à 0,92 avec une moyenne de 0,53 ± 0,01 pour l'ensemble des obtentions, de 0,16 à 0,79 avec une moyenne de 0,43 ± 0,02 pour celles tolérant la sécheresse, et de 0,16 à 0,92 avec une moyenne de 0,57 ± 0,01 pour celles tolérant la salinité. Le contenu de matériel polymorphe variait de 0,05 à 0,50 avec une moyenne de 0,30 ± 0,01 pour l'ensemble des obtentions, de 0,13 à 0,50 avec une moyenne de 0,37 ± 0,01 pour celles tolérant la sécheresse, et de 0,07 à 0,50 avec une moyenne de 0,29 ± 0,01 pour celles tolérant la salinité. L'analyse des grappes et des composantes principales révèle l'existence de groupes distincts parmi les obtentions tolérant la sécheresse et celles tolérant la salinité. Ces obtentions présentent une importante diversité génétique et constitueraient du matériel d'une grande utilité pour la sélection de variétés de blé tolérant à la fois la sécheresse et la salinité.Mots clés: AFLP, identification par le code génétique, diversité génétique, blé synthétique Abiotic stresses such as drought and salinity are major global constraints to wheat production. Approximately 32% of the wheat-growing regions in developing countries go through some drought stress during the growing season (Morris et al. 1992). In total, around 45 million ha of wheat-producing land are characterized by periodic drought stress (Byerlee and Moya 1993). Soil salinity causes significant reductions in plant productivity, and consequent economic losses associated with reduced grain quality and yield of agricultural crops (Pitman and Lauchli 2002).Genetic diversity is the foundation for genetic improvement of various crops. Genetic diversity of germplasm including those naturally occurring or synthesized can be assessed through pedigree analysis (Cox et al. 1985;Martin et al. 1991) and DNA markers (Autrique et al. 1996;Karp et al. 1997; Barrett and Kidwell 1998;Davila et al. 1998;Soleimani et al. 2002;Sasanuma et al. 2002). DNA markers, however, provide a direct measurement of genetic relationships among samples analyzed based on their genome composition. Also, an unlimited number of markers is available for such analyses. Therefore, DNA markers are a necessary complement to pedigree analysis.Based on restriction fragment length polymorphisms (RFLPs) in 113 improved cultivars and landraces of durum wheat (T. turgidum L.), Autrique et al. (1996) reported a mean genetic distance of 0.21 and 0.31 within the improved lines and landraces, respectively. Using AFLP markers Barrett and Kidwell (1998) studied genetic diversity among wheat (T. aestivum L.) cultivars from the US Pacific Northwest, and reported mean genetic diversity estimates ranging from 0.49 to 0.58 for within spring and winter types and between spring vs. winter types. Manifesto et al. (2001) used AFLP and simple sequence repeat (SSR) markers and quantified the genetic diversity among 105 modern and older bread wheat cultivars from Argentina concluding that Argentinian bread wheat germplasm had maintained a relatively constant level of genetic diversity during the last half century. Soleimani et al. (2002) detected a substantial amount of genetic variation between and within cultivars in 13 registered modern Canadian durum wheat cultivars based upon AFLP markers, reporting a mean pair-wise genetic distance of 0.40. Sasanuma et al. (2002) studied genetic diversity of wheat wild relatives from the Near East using AFLP, and reported the existence of potential genetic diversity among the wild relatives in natural populations. Lage et al. (2003) reported genetic diversity using AFLP and agronomic traits among 54 synthetic hexaploid wheats derived from crosses between emmer wheat (T. dicoccum L.) and goat grass (Aegilops tauschii Coss.). Based on AFLP, they observed clear grouping according to geographical origin for the T. dicoccum parents, but no clear groups for the Ae. tauschii parents. The hexaploid synthetics also revealed similar clustering as the T. dicoccum parent. Based on percentage polymorphic markers, the synthetic hexaploid wheats showed a considerably higher level of AFLP diversity (39%) than normally observed in cultivated wheat (12 to 21%).Reports on molecular marker studies of genetic diversity among wheat germplasm with drought tolerance are limited. Moghaddam et al. (2005) studied genetic diversity in bread wheat genotypes for tolerance to drought using AFLPs and agronomic traits such as plant height, days to flowering, days to maturity, grain yield and harvest index. Their study included 14 wheat genotypes from Iran and 14 wheat genotypes developed by or obtained by the International Maize and Wheat Improvement Center (CIMMYT). They reported that the genetic basis of drought tolerance of these accessions was different, particularly when comparing Iranian and CIMMYT accessions. No report was available on mol-ecular marker studies of genetic diversity among salinitytolerant wheat germplasm. However, Lindsay et al. (2004) reported an SSR marker closely linked to Nax1, a locus for sodium exclusion that gives salt tolerance in durum wheat, mapped on chromosome 2AL.Deoxyribonucleic acid (DNA) markers are the most suitable means for genetic diversity estimation (O'Donoughue et al. 1994;Plaschke et al. 1995;Kim and Ward 1997). However, the extent of their utility may depend on the type of the DNA markers, level of polymorphisms they reveal, and their genome coverage. Markers that can detect higher levels of polymorphism between wheat varieties can be utilized more efficiently to estimate genetic diversity. AFLP is such a marker class that can generate high levels of polymorphisms even in low polymorphic species like wheat (Bai et al. 1999). It is a multiplex marker system in which several polymorphic bands can be produced per assay (Vos et al. 1995). In addition, AFLP analysis is highly reproducible making it a suitable marker system for genetic diversity analysis and high-resolution mapping (Zabeau 1993).Since genetic diversity for traits such as drought and salinity tolerance is limited in conventional wheat, introgression of genes from wild relatives into elite cultivars has been a major wheat-breeding program objective within CIMMYT. Aegilops tauschii Coss. (2n = 2x = 14, DD), a diploid wheat relative, is a rich source of resistance genes to many biotic and abiotic stresses (Mujeeb-Kazi and Rajaram 2002). Synthetic hexaploid wheats (2n = 6x = 42, AABB-DD), which are generated by crossing tetraploid durum wheat (2n = 4x = 28, AABB) to various Ae. tauschii accessions, are an important bridging material for introgression of desirable genes from Ae. tauschii into bread wheat. CIM-MYT has developed numerous synthetic hexaploid wheats using diverse durum cultivars and Ae. tauschii accessions. These synthetic wheats possess many other important biotic stress traits that can be utilized for wheat improvement (Mujeeb-Kazi 2003a), such as resistance to Fusarium head blight (Fusarium graminearum Schw.), leaf rust (Puccinia triticina Eriks.), Septoria tritici blotch (Septoria tritici Roberge in Desmaz.) (Mujeeb-Kazi et al. 2000), Karnal bunt (Tilletia indica Mitra) (Mujeeb-Kazi et al. 2001a) (Cox 1998;Xu et al. 2004). A set of germplasm with high levels of tolerance to drought and saline environments has also been identified (Reynolds et al. 2005). The objectives of the present study were (i) to evaluate genetic diversity of the selected germplasm sets that contain different D genome accessional sources conferring tolerance to drought and salinity based on AFLP and (ii) to identify parents for developing doubledhaploid-based mapping populations (Mujeeb-Kazi 2003b).Wheat accessions in this study include 14 accessions (drought accessions) with different levels of drought toler-ance and 27 accessions (salinity accessions) with different levels of salinity tolerance. The drought accessions consisted of five conventional wheat cultivars, five synthetic hexaploid wheats, and four durum wheat cultivars that were parents of the respective synthetics (Table 1). Entries D1 to D5 are the synthetics with superior drought tolerance and are currently being used for drought tolerance wheat breeding in CIMMYT. Their advanced derivatives after crosses with bread wheat cultivars have also performed well under reduced irrigation. Among the five conventional wheat cultivars, cv. Opata is a susceptible check and an ideal drought sensitive parent for developing mapping populations. The salinity accessions consisted of 19 conventional wheat cultivars, seven synthetic hexaploid wheats and one durum wheat cultivar (Table 2). The durum wheat (PDW 34) and wheat cultivars Oasis, PBW 343, Galvez S87 and Yecora F70 are salinity-susceptible, while the rest of the accessions are salinity tolerant. The salinity-tolerant accessions have been selected based on potassium: sodium (K:Na) discrimination levels in hydroponics using protocols of Gorham et al. (1987) and Shah et al. (1987). All salinity-tolerant accessions had K:Na ratios over 2.5, where a ratio of close to 1.0 indicates salt sensitivity. Entry S5 (PDW 34), the durum susceptible check had a K:Na value close to 1.0. The synthetic wheats differed not only in sources of D genome, but also in their A and B genome compositions. The latter durum parent in every case was salt susceptible (Pritchard et al. 2002).Genomic DNA was isolated from bulked wheat leaves of two to three seedlings (approximately 10 d old) using the CTAB procedure (Saghai-Maroof et al. 1984). AFLP analysis was performed using protocols described by Zabeau (1993) and Vos et al. (1995). Laboratory optimization and minor modifications for AFLP analysis were made according to Bai et al. (1999). Genomic DNA (300 ng) from each of the wheat entries was double digested with EcoRI and MseI restriction enzymes. Following restriction digestion, EcoRI and MseI adapters were ligated to the digested DNA fragments. Ligated DNA was diluted 10-fold for pre-amplification. Forty micro-liters of PCR reaction mixture contained 10 µL of the diluted DNA, 4 µL of 10X PCR buffer, 4 µL MgCl 2 (25 mM), 1.6 µL dNTPs (5 mM), 0.75 µL EcoRI pre-amplification primer (100 ng µL -1 ), 0.75 µL MseI pre-amplification primer (100 ng µL -1 ), 0.15 µL Taq polymerase and 18.75 µL of deionized water. Pre-amplification PCR was done in a MJ thermocycler (MJ Research Inc., Waltham, MA) with the following thermal profile: 94°C for 1 min followed by 30 cycles at 94°C for 30 s, 56°C for 60 s and 72°C for 60 s. The PCR product was then analyzed on 1.5% agarose gel to confirm pre-amplification.The pre-amplified DNA was diluted 10-fold. The PCR reaction mixture for selective amplification included 2 µL of the diluted DNA, 1 µL of 10X PCR buffer, 1 µL of 25 mM MgCl 2 , 0.4 µL of 5 mM dNTPs, 0.35 µL of MseI selective primer (50 ng µL -1 ), 0.4 µL fluorescence-labeled EcoRI selective primer (1 ρmol µL -1 ) from LI-COR (LI-COR Inc, Lincoln, NE), 0.04 µL Taq polymerase, and 4.8 µL deionized water. The PCR thermal cycles were as follows: 2 min at 94°C followed by 13 cycles at 94°C for 30 s, 65°C for 30 s, 72°C for 60 s with the annealing temperature lowered by 0.7 °C after each cycle; then followed by 23 cycles at 94°C for 30 s, 56°C for 30 s, 72°C for 60 s. A final extension was conducted for 5 min at 72°C. Twenty AFLP selective primer combinations were used for selective amplification (Table 3).The PCR products from the selective amplification were mixed with 5 µL of loading buffer and denatured for 5 min at 95°C before 1 µL of this product from each sample was loaded on each well of a 6.5% denaturing Gel Matrix gel (Li-Cor Inc., Lincoln, NE). The gel was ran in 1X TBE buffer at 1500 V and 40W for 3.5 h in a Li-Cor automated DNA sequencer (Li-Cor Inc., Lincoln, NE). DNA size standard from Li-Cor was used as a reference to calculate molecular size of each AFLP fragment.AFLP bands ranging from 70 to 350 base pairs were scored as present (1) or absent (0). Unambiguous fragments were entered as 0.5 in the data matrix. In order to ensure accurate scoring, all markers were scored at least twice. Polymorphism rates were estimated for all possible pairs of lines by dividing the number of polymorphic bands by the total number of bands. Polymorphic information content (PIC) was estimated using the formula used by Anderson et al. (1993):where p i is the frequency of the ith allele. Each polymorphic fragment was scored as a locus with two allelic classes. The maximum PIC value of an AFLP locus was 0.5. Cluster analysis and principal components analysis was conducted using the procedures in the NTSYS-pc software (Rohlf 2000). Genetic similarity between entries was estimated using the similarity coefficients of Jaccard (1908), Rogers and Tanimoto (1960) and Nei and Li (1979). The resulting distance matrices were used for cluster analysis by the UPGMA [unweighted pair-group method with arithmetic averages (Sneath and Sokal 1973)] method. The goodness of fit of the clustering to the data matrix was assessed by cophenetic correlation using the NTSYS-pc software (Rohlf 2000).Analyses of 41 wheat entries (14 for drought and 27 for salinity) with 20 AFLP primer combinations produced a total of 918 scorable AFLP fragments (Table 3). A partial AFLP image generated from Li-Cor 4200 DNA analyzer showing typical AFLP variation when a single pair of AFLP primers was used for selective amplification is shown in Fig. 1. Of the 918 markers, 368 were polymorphic among all 41 wheat entries (drought and salinity entries combined) studied, 348 were polymorphic among the drought entries, while 310 were polymorphic among the salinity entries. For all 41 entries, the number of polymorphic bands per primer combination ranged from 8 to 33 with an average of 18.4 ± 1.5 (Table 3). For the drought germplasm, the number of poly-morphic bands per primer combination ranged from 8 to 30 with an average of 17.4 ± 1.4, while for the salinity entries, the number of polymorphic bands ranged from 6 to 26 with an average of 15.5 ± 1.4 per primer combination. Polymorphism rates were estimated at 40, 38, and 34% for all 41 entries, drought entries, and salinity entries, respectively.Pair-wise comparisons were conducted between the genotypes based on the AFLP data. Jaccard's similarity coefficient was used to evaluate the genetic diversity among the accessions. Within all 41 entries, the highest similarity coefficient (0.92) was between synthetics S22 and S25 and the lowest similarity coefficient (0.18) was between the drought entry D7 and the salinity entry S5 (the salinity-susceptible durum wheat check). The average similarity coefficient for all 41 entries was 0.53 ± 0.01. The result from pair-wise comparisons indicated that about 37% of the entry pairs had similarity coefficients of 0.50 or less (Fig. 2). Within the drought entries, the highest similarity coefficient (0.79) was between synthetics D2 and D4 although they were unrelated based on their pedigree information. The lowest similarity coefficient (0.16) was between entry DP2 (a durum wheat) and D7 (a conventional wheat). The average similarity coefficient for drought entries was 0.43 ± 0.02. Pair-wise comparisons indicated that more than half of the entry pairs had similarity coefficients of 0.50 or less (Fig. 3). Within the salinity entries, the highest similarity coefficient (0.92) was between synthetics S22 and S25 and the lowest similarity coefficient (0.16) was between the entry S5 (the salinitysusceptible durum wheat check) and S6 (a conventional wheat cultivar), with an average similarity coefficient of 0.57 ± 0.01. About 31% of the pair-wise comparisons had similarity coefficients of 0.50 or less (Fig. 4).For all 41 entries, PIC ranged from 0.05 to 0.50 with an average PIC of 0.30 ± 0.01 (Fig. 5). For the drought entries, PIC ranged from 0.13 to 0.50 with an average PIC of 0.37 ± 0.01 (Fig. 6), while for the salinity entries, PIC ranged from 0.07 to 0.50 with an average PIC of 0.29 ± 0.01 (Fig. 7). PIC of AFLP in drought entries was high and mainly appeared between 0.40 and 0.50 (Fig. 6), while PIC of AFLP in salinity entries was relatively lower with two peaks appearing in both distribution extremes (Fig. 7).In general, high cophenetic correlations ranging from 0.873 to 0.944 were obtained (Table 4) where r > 0.9 indicates a very good fit; 0.8 < r < 0.9 indicates a good fit; r < 0.8 indicates a poor fit (Capo-chichi et al. 2001). All three methods used in these analyses gave a good fit with the best cophenetic correlation from the Jaccard coefficient (Table 4). No major variations were observed in the dendrogram patterns obtained by the three similarity coefficients; therefore, only the dendrogram obtained based on the Jaccard coefficients is presented. The dendrogram involving all 41 wheat accessions grouped the accessions into three main clusters (Fig. 8). Cluster one consisted of all five durum entries used in this study [one from the salinity entries (S5) and four from the drought entries (DP1 to DP4)]. The second cluster consisted of all conventional wheat entries (D6 to D10) from the drought entries and 15 of the 19 conventional wheat entries and one synthetic wheat entry from the salinity entries. The third cluster had all the five synthetic wheat entries (D1 to D5) from the drought entries and five of the six synthetic wheat entries from the salinity entries. This cluster also contained four conventional wheat entries from the salinity entries. The similarity matrix based on the Jaccard's coefficient was also used as input for principal component analyses (PCA). A plot of the first three principal components for all 41 accessions is presented in Fig. 9. The results from PCA are similar to those obtained by UPGMA clustering (Fig. 8). The five durum entries separated from wheat at the first principal component (PC1), and the second principal component (PC2) contained the cluster consisting mainly of the conventional wheat entries. The third principal component (PC3) contained the cluster consisting mainly of the synthetic wheat entries. The genetic constitution of the synthetics was different from the conventional wheat cultivars studied, thus suggesting that synthetics would add diversity to the drought-and salinity-tolerant germplasm.The dendrogram for the drought accessions grouped the accessions into two main clusters (Fig. 10). Cluster one consisted of the four durum parents (DP1 to DP4). Cluster two consisted of all five synthetics and five conventional wheat lines. The second cluster formed two sub-clusters that were separated at the 46.5% similarity level with one sub-cluster consisting of the five conventional wheat lines and the other consisting of the five synthetics. Durum parents were distant from both the synthetics and the conventional wheat lines. The plot of the first three principal components for the drought accessions is presented in Fig. 11. The results from PCA are similar to those obtained by UPGMA clustering (Fig. 10). The four durum entries separated from conventional and synthetic wheat entries at the first principal component (PC1), and conventional wheat entries separated from synthetics at the second principal component (PC2). The third principal component (PC3) separated individual accessions within each major cluster. The clustering of the drought entries also indicated that genetic constitution of synthetics was different from the conventional wheat cultivars studied and thus the synthetics would add more diversity to the drought-tolerant germplasm.The dendrogram for the salinity accessions (Fig. 12) shows that this clustering method grouped 26 of the 27 entries into two major clusters while entry 27 (S5, the salinity-susceptible durum wheat check) remained by itself. The two major clusters merged into one cluster at the similarity level of 50.4% and the susceptible durum check (S5) merged with this cluster at the similarity level of 28%. The durum check lacks the D genome; therefore, it is genetically distant from the synthetics and conventional wheat cultivars. Of the two major clusters, one cluster consisted of six of the seven synthetics and four conventional wheat lines. Another cluster consisted of one synthetic and the remaining conventional wheat lines. The PCA results (Fig. 13) also support the results obtained by UPGMA cluster analysis (Fig. 12). PC1 separated two major distinct groups of wheat, while PC2 clearly separated the durum check (S5) from the two wheat groups. PC3 separated individual accessions within each major group consisting of both conventional wheat cultivars and synthetics.Synthetic wheat lines developed at CIMMYT have many valuable traits including drought and salinity tolerance that can be used for genetic improvement of wheat. The present study was aimed at measuring genetic diversity among several drought-and salinity-tolerant synthetic and conventional wheat lines using AFLP markers and to identify parents for developing doubled-haploid-based mapping populations (Mujeeb-Kazi 2003b). Twenty primer combinations used in this study revealed substantial genetic diversity among the drought-tolerant and salinity-tolerant wheat germplasm.When all 41 wheat accessions were analyzed together, the average similarity coefficient was 0.53 with the lowest similarity coefficient being 0.18. Within the drought entries, the average similarity coefficient was 0.43 with the lowest similarity coefficient being 0.16. The lowest similarity coefficient for the salinity entries was the same as that for drought entries, but the average was higher (0.57). These results and the distribution of the similarity coefficients indicated substantial amounts of genetic diversity among the drought and salinity entries. Based on 117 polymorphic AFLP markers, Bohn et al. (1999) reported genetic similarity among 11 wheat lines ranging from 0.40 to 0.83 with an average similarity of 0.61. Using AFLP markers Barrett and Kidwell (1998) reported mean genetic diversity estimates of 0.58 for pair-wise comparison of spring vs. winter wheat, 0.53 for within winter wheats and 0.49 for within spring wheats. Soleimani et al. (2002) using AFLP markers reported a mean pair-wise genetic distance of 0.40 for several Canadian durum wheat cultivars.Our results of the average PIC (0.30 ± 0.01 for all 41 entries, 0.37 ± 0.01 for drought and 0.29 ± 0.01 for salinity entries) were similar to that of Manifesto et al. (2001) who reported an average PIC value of 0.30 ± 0.15 in wheat for AFLP markers. However, the range of PIC values (0.26 to 0.38) reported by Manifesto et al. (2001) were smaller than the ranges of PIC values (0.05 to 0.50 for all 41 entries, 0.13 to 0.50 for drought entries and 0.07 to 0.50 for salinity entries obtained in our study. PIC is a quantification of the number of alleles or bands that a marker has and the frequency of each of the alleles or bands in the entries under study. Since a marker with fewer bands has less power to distinguish between entries and alleles present at low frequency also have less power to be distinguished, a higher PIC was assigned to a marker with many alleles and with alleles present at roughly equal proportions in the entries under study. Thus, PIC can be looked as the measurement of usefulness of each marker in distinguishing one individual from another. For AFLP, each polymorphic fragment was scored as a locus with two allelic classes and the maximum PIC of an AFLP locus is 0.5. Therefore, the PIC values in our study indicated that the AFLP markers were reasonably powerful in distinguishing one individual from another. This was in congruence with Manifesto et al. (2001).Dendrograms (Figs. 8, 10, and 12) obtained by cluster analysis using UPGMA and Jaccard's similarity coefficient show that there was substantial diversity at the DNA level among the drought-tolerant and salinity-tolerant germplasm. When all the 41 entries were used in the cluster analysis, the five durum entries were grouped together as expected. One cluster contained all conventional wheat and only one synthetic wheat line and the other cluster contained mainly synthetics but some conventional wheat entries. This indicated that most of the synthetics were distant from the conventional wheats. These two later clusters contained both salinity and drought entries. This indicated that these drought and salinity entries had enough genetic similarity to cluster together. For the drought entries, the four parents were grouped together in both PCA and cluster analysis, that was expected because they were all durum entries with AABB genomes. It was interesting to note that four of the synthetics developed from these four parents by crossing each to a different Ae. tauschii accession were closer to each other, suggesting that genetic constitution of different Ae. tauschii accessions involved in these crosses could be similar to each other. The two sub-clusters of the drought accessions merged at the 46.5% similarity level indicating that they were distantly related at the genetic level. It was interesting to note that one of these two sub-clusters contained the five conventional wheat lines and the other contained the five synthetics. Although both the conventional wheat lines and the synthetics have the same genome, AABBDD, they exhibited differences due to their earlier conventional and recent synthetic origin. This also indicated that AFLP markers were powerful enough to reveal such genetic distinction. It would be possible to select appropriate drought-tolerant parents from both groups for a breeding program to enhance genetic diversity for drought tolerance. Our results are in congruence with those of Moghaddam et al. (2005) who reported existence of genetic diversity among drought-tolerant wheat entries from CIMMYT and Iran.The salinity entries formed two major clusters that merged at the similarity level of 50.4%. These two groups were clearly separated by the second principal component. The result indicated a high level of genetic diversity among these entries. Using these entries to breed for salinity tolerance would enhance genetic diversity of salinity-tolerant cultivars. As expected, accession S5, the salinity susceptible durum wheat check stood alone in both the dendrogram and the PCA plot and merged with the other accessions at the similarity level of 28%. This result again indicated that AFLP fingerprinting provided a very accurate measurement of genetic relationship among diverse entries. Both synthetics and conventional wheat entries in each of two sub-clusters indicated substantial genetic diversity within the salinity-tolerant wheat and synthetic accessions.The synthetic and conventional wheat entries used in this study have shown different degrees of tolerance to drought and salinity. Genetic diversity assessment using AFLP has clearly indicated that there exists ample diversity among these entries at the DNA level and therefore some entries would be very valuable when breeding objectives target drought and salinity tolerance. Some of the entries would be valuable for using as parents for developing doubledhaploid-based mapping populations.","tokenCount":"4596"} \ No newline at end of file diff --git a/data/part_6/0590433744.json b/data/part_6/0590433744.json new file mode 100644 index 0000000000000000000000000000000000000000..659e15accc9cc12a22a9266c8bb075dc6e79d4fc --- /dev/null +++ b/data/part_6/0590433744.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"3ee54352e75dff628c1a1a58c334d36a","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/883451e0-8fbe-4a87-8550-e9e691bb0f97/retrieve","id":"1116396963"},"keywords":[],"sieverID":"519f43ea-1e61-4e99-97a6-d11b7e2a33eb","pagecount":"9","content":"Sterility and low seed set in bananas is the main challenge to their conventional genetic improvement. The first step to seed set in a banana breeding program depends on pollination at the right time to ensure effective fertilization. This study aimed at determining bract opening time (BOT) to enhance efficient pollination and seed set in bananas. A Nikon D810 digital camera was set-up to take pictures of growing banana inflorescences at five-minute intervals and time-lapse movies were developed at a speed of 30 frames per second to allow real-time monitoring of BOT. Genotypes studied included wild banana (1), Mchare (2), Matooke (4), Matooke hybrid (1), and plantain (1). Events of bract opening initiated by bract lift for female flowers (P < 0.01) started at 16:32 h and at 18:54 h for male flowers. Start of bract rolling was at 18:51 h among female flowers (P < 0.001) and 20:48 h for male flowers. Bracts ended rolling at 02:33 h and 01:16 h for female and flowers respectively (P < 0.05).Total time of bract opening (from lift to end of rolling) for female flowers was significantly longer than that of male flowers (P < 0.001). On average, the number of bracts subtending female flowers opening increased from one on the first day, to between one and four on the fourth day. The number regressed to one bract on day eight before start of opening of bracts subtending male flowers. There was a longer opening interval between bracts subtending female and male flowers constituting spatial and temporal separation. Bract rolling increased from partial to complete rolling from proximal to the distal end of the inflorescence among female flower. On the other hand, bracts subtending male flowers completely rolled. Differences in BOT of genotypes with the same reference time of assessment may be partly responsible for variable fertility. Hand pollination time between 07:00 and 10:00 h is slightly late thus an early feasible time should be tried.Banana (Musa spp.) plants are monoecious where male and female flowers are primarily unisexual and separated on the same inflorescence 1 . They have a year-round flowering habit implying that pollinations can be made all year 2 . The inflorescence develops in the pseudostem after which leaves are replaced by bracts. The first three or four bracts are the largest and typically do not bear flowers. After emergence, the inflorescence takes on a horizontal or pendent position for edible bananas. Female flowers emerge before male flowers and are usually separated by neutral flowers 3 . This implies that there is no self-pollination within an inflorescence except in some genotypes with hermaphrodite flowers 4 . Geitonomous pollination can occur between flowers in different generations on the same mat.Each bract bears two rows of flowers in a cluster, one above the other. Similar to most monoecious plants, bananas produce more male than female flowers. The flowers are irregular with a compound tepal, androecium and gynaecium as the three main parts. These flower parts are all joined at the point of connection of the style with the ovary thus form an inferior ovary 5 . Depending on genotype, soil fertility, and environmental conditions, the inflorescence usually bears between 1 to 30 female flower clusters, followed by 0 to 4 neutral flower clusters and up to 300 male flower clusters 1 .Rapid conventional improvement of bananas is hindered by a complex array of factors that result in male and female sterility 6 . Among factors that influence seed set especially for controlled pollination is pollinating at the right time of the day to ensure maximum seed set 7 . Flowers must be pollinated soon after opening as ovules start to disintegrate 24 h after anthesis 1 . On the other hand, pollen viability is highest at 08:00 h and lowest at 16:00 h 8 . Shepherd 7 obtained the highest seed set in pollinations made at 07:00 h and the lowest after pollinating at 16:00 h. A thorough understanding of banana floral biology could therefore contribute toward solving the poor seed set problem in edible banana. Increase in seed set per cross made would create a wider progeny base for breeders to make meaningful selections and consequently efficient breeding pipelines 6 .Flower opening time is a tightly regulated trait in plants, and this determines when and which pollinators are involved in the pollination process. This in turn determines the fitness of a plant species to survive. Banana is a facultative day long plant with long photoperiods leading to early inflorescence initiation 9 . Bract opening at night implies that bats participate in pollination, although diurnal insects have also been reported to visit flowers 10 . The exact start of bract opening time (BOT) is unclear in banana, as opposed to crops such as rice, where flower opening time is between 09:00 and 14:00 h 11 . A case study by Amah et al. 6 found that bract lift in Mchare bananas in Arusha Tanzania started late afternoon but appearance of flowers was after night fall. The study also found that there is maximum flower visitation frequency at dawn which is believed to be the period of maximum stigma receptivity.Maximum seed set was obtained after controlled pollination of 'Gros Michel' between 07:00 and 10:00 h 7 and this has been widely adopted 6,7 . In rice, flower opening time is known to be controlled genetically by about three genes, but weather conditions have also been observed to have an influence 11 . Weather is likely to trigger flower opening so that pollination happens under favourable night conditions. Since the widely adopted pollination time range is between 07:00 to 10:00 h, the assumption would be no differences in BOT among banana genotypes. Also, female and male flowers are separate in banana and there is no information on whether opening is synchronized on separate plants. The aim of this study was therefore to determine BOT in banana under field conditions and how BOT is influenced by weather conditions.Field site and banana genotypes used. The experiment was conducted at two sites in Uganda; the National Agricultural Research Laboratories (NARL) in Kawanda and the International Institute of Tropical Agriculture (IITA) Sendusu station, Namulonge. Kawanda is located at 0° 25′ N and 32° 32′ E at an elevation of 1177 m, while Namulonge is located 0° 31′ N and 32° 36′ E at an elevation of 1160 m. Banana genotypes used included Musa (AAA-EA group Matooke subgroup) 'Enzirabahima' , 'Nakitembe' , 'Enyeru' , 'Kabucuragye' 12 , and 'NARITA 17' (AAA) which is a Matooke hybrid from collaborative breeding efforts between NARL and IITA 13 . Also used were Musa (AA group subgroup Mchare) 'Mlelembo' and 'Kamunyilya' and Musa (AAB group subgroup Plantain) 'Gonja' for a B-genome representation. Letters \"A\" and \"B\" in groupings denote contributions from progenitors Musa acuminata and M. balbisiana respectively. Wild banana M. acuminata spp. burmannicoides 'Calcutta 4' was also included.At NARL, 'Enzirabahima' , 'Nakitembe' , 'Mlelembo' , 'Kamunyilya' , and 'Calcutta 4' were studied between July 25 and September 27, 2016 whereas 'Enyeru' , 'Kabucuragye' , 'NARITA 17' , and 'Gonja' were studied at IITA between September 20 and November 11, 2018. Observations were made on already established plants in pollination blocks planted at a spacing of 3 × 2 m. For each genotype, a single inflorescence was studied. Cultivars in the Matooke and Mchare subgroups were treated as replicates since variability within these subgroups are said to have arisen from somatic variation 14,15 . The subgroups studied were therefore; Mchare (2), Matooke (4), Matooke hybrid (1), plantain (1), and wild banana (1).Because banana plants usually grow to a height of more than 3.0 m, a table of 1.0 m was used to supplement the camera tripod stand which could go up to a 2.0 m height. The tripod stand was firmly bound on the table surface to avoid movement and shaking of the camera. A Nikon D810 camera (Nikon Corporation, Tokyo, Japan) was used to take photographs of the growing banana inflorescences at an interval of 5 min. The camera flash was set to manual mode with highest power and fastest sync speed; it was left on throughout the study period. The period of flower study started when the inflorescence was in the erect position and pictures were taken continuously until opening of a few bracts subtending male flowers. With MatLab Version 9.4 (R2018a) software developed by MathWorks (https:// www. mathw orks. com/), timelapse videos were made at a speed of 30 frames per second (fps). The videos were played using Kinovea Version 0.8.15.0 software (https:// www. kinov ea. org/) developed by Joan Charmant and time for bract opening events were recorded. Bract opening events included time of bract lift, time of start and time of end of bract rolling. Bract lift implied the loosening of the bract due for opening on the tightly packed flower bud while start of bract roll time was when the tip of bract started rolling backwards. Bract roll rate was determined by subtracting time of end of bract rolling from time of start of bract rolling. Total time of bract opening events was calculated by subtracting time of end of bract rolling from bract lift time. Events time for each bract were converted to fractions (hours) and averaged separately for bracts subtending male and female flowers for each inflorescence. Data were subjected to one-way analysis of variance without blocking with subgroups as treatments; data for bracts subtending female and male flowers were analysed separately. Total time of bract opening events for bracts subtending female and male flowers was also compared in a paired t-test with genotypes constituting pairs. Analysis of variance and the t-test were run using Genstat for Windows 19th edition (http:// www. genst at. co. uk) developed by VSN International (VSNi).Irrespective of when the bract started to open, time-lapse videos were paused at 08:00 h for each bract to measure the angle of bract lift with reference to the rachis for both female and male flowers. Bract roll was scored on the scale of 1 for minimal roll to 5 for complete bract roll. Bracts events that could not be observed for partially or fully obscured bracts were recorded as missing data. Time duration between bracts subtending female and male flower opening was calculated as time of lift of first bract subtending male flowers minus bract roll end-time of last bract subtending the female or transition flower cluster. Average bract roll scores and lift angles per cluster position were plotted against bract position number. For genotypes whose single bract events happened before and after 00:00 h, total hours from 00:00 h of the previous day were counted and averaged.Weather data were obtained from the NARL and the Namulonge agro-metrological stations. The Namulonge agro-metrological station is 1.5 km from the IITA -Sendusu station thus data were used for the latter station because of their proximity. From the NARL station, we obtained temperature (°C) data recorded at 15:00 h whereas from the Namulonge station, we obtained average daily temperature (°C) and light intensity (lux). During the study period, temperature at 15:00 h at NARL ranged from 23.5 to 32.0 and averaged 29.0 °C. On the other hand, daily temperature at Namulonge was in the range of 20.5 and 24.6 with an average of 22.8 °C while light intensity ranged between 1167.7 and 3316.2 with an average of 2263.1 lux. Correlation analysis was performed between bract opening events time and weather data. The analysis was performed with Genstat for Windows 19th edition (http:// www. genst at. co. uk) software developed by VSNi.The authors confirm that the banana genotypes used in this present study was in accordance to international, national and/or institutional guidelines.Bract opening events. Banana bract opening events were generally initiated by bract lifting followed by bract rolling (Table 1). In some cases, bract lifting and bract rolling were simultaneous events, especially for the first three female clusters and particularly for 'Mlelembo' . In observed subgroups, Matooke had the earliest lift and start of rolling of bracts while wild banana 'Calcutta 4' lifted and started rolling its bracts last (Table 1). Bract lift and start of rolling was statistically significant for bracts subtending female flowers but not for those of male flowers. And end of bract rolling happened first in Matooke and last in Plantain; this was statistically significant for both bracts subtending female and male flowers.On average, lift and start of rolling events for bracts subtending female flowers happened before events for bracts subtending male flowers, the exception was end of bract rolling. Bract rolling rates among female flowers were not statistically significant, results for male flowers were not analysed as there were many data missing. Total duration of bract opening was not significant among female flowers but was significant among male flowers. Total duration of bract opening events of female flowers was more than that of male flowers (paired T-test prob. > 0.001, 6 d.f.).With the exception of 'Gonja' , all observed inflorescences exhibited a tendency of partial rolling of bracts subtending female flowers a day or two before actual lift and roll (Fig. 1a,b). This happened to a varying degree from proximal to distal end of the inflorescence in different genotypes, especially in the first three to four bracts. On the other hand, bracts subtending male flowers did not exhibit partial rolling of bract tips before fully lifting and rolling in subsequent days (Fig. 1c,d). It was observed that bracts that had not completed rolling and curling of fingers backwards were halted when day broke. The process continued on subsequent days simultaneously with lifting and rolling of fresh bracts. By the time female and male flowers were visible, the compound and free tepals had already opened to expose female and male flower parts in all genotypes studied. In some genotypes especially 'Enyeru' and 'Kabucuragye' , inflorescences moved from the horizontal-pendent position towards the horizontal position at dusk and fell towards the pendent position by mid-morning. This was a repetitive process during bract opening period of female flowers.On average, more bracts subtending female flowers opened per day in the mid-section of the inflorescence compared to proximal and distal ends (Fig. 2). One bract subtending female flowers opened on the first day with maximum number of bracts opening on day four. This gradually reduced up to one bract opening on day eight which was the last day of bract opening for female flower clusters. All bracts subtending female flowers opened in a period of three to eight days (Table 1). In the event of multiple bracts opening on the same day, former clusters had flowers with brownish stigmas while latter clusters had flowers with creamy stigmas. And for multiple bracts opening on the same day, opening events were simultaneous or were separated by up to four and a half hours with former bracts opening first. After opening of bracts subtending female and transitional clusters, there was on average a longer lapse before the onset of opening of bracts subtending male flowers. The longest lapse period was in 'Mlelembo' while 'Kamunyilya' , 'Enzirabahima' , and, 'NARITA 17' had lapses of less than a day (Table 2). Lapse duration of less than a day between male and female flowers was the same as the opening interval of bracts subtending female flowers.Bracts subtending female flowers at the proximal end of the inflorescence partially rolled, the degree of rolling gradually increased towards the distal end (Figs. 1a, 3). Bracts subtending female flowers at the distal end completely rolled, which was comparable to bracts subtending male flowers. On the other hand, bracts subtending male flowers had a relatively similar appearance after rolling irrespective of the bract position (Fig. 3). The angle of bract lift at 08:00 h was also small in proximal clusters but gradually increased towards the distal end of the inflorescence (Fig. 4). This was generally exhibited by all observed inflorescences. On average, bracts subtending female flowers in position one lifted to about 25° while bracts in position eight lifted to more than 60° by 08:00 h. Male flowers were observed to have more insect visitors compared to female flowers especially between dawn and about 08:00 h.Influence of weather on bract opening. Average daily temperature had no significant relationship with time of bract opening events for both female and male flowers at the IITA-Sendusu station (Table 3). There was also no relationship between temperature recorded at 15:00 h and bract opening events time at NARL station (data not presented). High light intensity led to late bract lift, start of rolling and end of rolling among bracts subtending female flowers (Table 3). High light intensity also led to a faster rate of rolling for bracts subtending female flowers, there was no relationship with total events time. On the other hands, high light intensity led to an early lift of bracts subtending male flowers.In banana, bract opening behavior depends on the time of the day, the position of the bract, and sex of the flowers enclosed by the bract. Bract opening is a continuous process especially in the first bracts subtending female flowers of some genotypes; it starts in the evening and continues through the night (Table 1). In cases where bracts did not fully open, the process was halted early morning and resumed in the evening. It is therefore not obvious to judge whether such bracts have opened or not. However, opening is permanent as opposed to some plant species which open and close their flowers at specific times. Ssebuliba et al. 16 considered East African Highland bananas ready for pollination when bracts were half way open with stigmas having a creamy white appearance. According to observations made in the current study, it can be said that bract lifting is indicative of flower opening thus pollination can start. Bract lift and bract roll seemed to be a response of a certain light quality 6 , the response time and speed are genotype dependent. Finger curling also seems to be triggered by the same factors that lead to bract opening. Bract opening and finger curling are likely to be a response of changes in turgor pressures in cells that lead to tissues being pushed in a given direction 17 . This was evident with upward movement of the inflorescence from the horizontal-pendent toward the horizontal position in the evening and downward movement towards the pendent position by mid-morning. These movements were genotype dependent and small, maximum oscillation was about 10˚. A similar pattern was observed for leaf folding to influence relative canopy cover 18 .Generally, bracts subtending female flower lifted and started rolling earlier than those subtending male flowers. However, male flowers ended opening before female flowers, resulting in faster bract opening for male flowers (Table 1 and t-test). This might be due to the smaller bract size of male flowers (Fig. 1) or an adaption for female flowers to find male flowers open with ready pollen. Consequently, the strategy ensures maximum pollination success and survival of the Musa spp. Studies have revealed that pollen viability reduces with time after flower opening 1 . This is in agreement that controlled pollination should be done between 07:00 and 10:00 h 7 . In comparison to lilies, some flowers were observed also to open starting at 17:00 h while others open during day. Both nocturnal and diurnal pollinators were found to be active flower visitors 19 . This implies that pollination in banana can start in the evening as long as bracts for parents in the cross of interest lift in time.In Musa itinerans, two nectar production peaks were found, that is between 08:00 to 12:00 h and 20:00 to 24:00 h 20 . This maybe a close depiction of what happens in edible bananas thus emphasizing the potential importance of diurnal and nocturnal pollinators. Bats, bees, and birds were found to be among the most important pollinators of bananas at Onne, Nigeria 10 . However, natural pollinators were not the main focus of the study though they are good indicators of when stigmas might be highly receptive. Since nectar quality and quantity varies between different agro-ecologies and seasons 21 , flower visitations and seed set are also expected to vary accordingly. Different agro-ecologies are also expected to experience variable BOTs due to variable solar radiation. Likewise the different growing seasons (rainy and dry) might also affect BOTs and therefore seed set 22 . However, a comparison of time from sunrise to beginning of bract lift of Musa AAA Cavendish cultivars in a glasshouse and M. basjoo in the garden in Belgium revealed no significant difference 6 . But comparison of bract curling time in Mchare in Arusha with short days and Cavendish cultivars in a glasshouse in Belgium with long days in summer, there was early curling in the glasshouse. However, bract lift time may be a better event to use for comparison than bract curling or rolling time.Bracts of both female and male flowers of different genotypes completed opening at different times and this may be partly the reason for variable pollen viability and stigma receptivity (Table 1). Female flowers that finish opening much earlier may set less seed compared to those that finish opening closer to the routine time of hand pollination between 07:00 and 10:00 h. Conversely, male flowers that are ready shortly before the time of hand pollination are expected to have higher pollen viability. This probably explains the high fertility of 'Calcutta 4' as it finished opening at 06:30 h. Some male flowers like those of Matooke finished opening as early as 21:54 h (Table 1) and are expected to have pollen with low viability at the time it is measured the next day.All observed inflorescences opened one female bract on the first day, increasing to multiple bracts opening on subsequent days (Fig. 2). One to three bracts subtending female flowers were observed to open per day from the second bract position of the inflorescence. The pattern of opening took on a hyperbolic shape with up to four bracts opening on the fourth day in the midsection of the inflorescence. For hand pollination, more clusters are therefore expected to be pollinated per day during bract opening in the mid-section of the inflorescence. The different clusters of female flowers that open on the same day are likely to have stigmas with varying receptivity. The darker appearance of stigmas of former clusters compared to creamy stigmas in latter clusters reflects higher receptivity in the latter 2 . This may explain why some clusters set more seed especially in the mid-section of a seemingly fertile inflorescence.Upon complete opening of female and transitional bracts, inflorescences went into a pause period before male flowers opened (Table 2). In additional to spatial separation of flowers, this is temporal separation to promote cross pollination in banana. However, temporal separation of male and female flowers is not very effective for genotypes that had less than 24 h of separation. With aid of crawling insects, self-pollination may happen between the last female cluster and the first male cluster as stigmas are likely to be receptive for more than one day. Once male flowers started opening, one bract opened per day and occasionally two bracts were observed to open on the same day. For highly fertile genotypes like 'Calcutta 4' , ample pollen is produced to pollinate many female flowers. Male flowers are also produced throughout the inflorescence growth period which ensures constant supply of pollen especially for controlled hand pollination. Averages of bracts subtending male flowers opening per day could not be calculated as there were two to three observed bracts subtending male flowers for most genotypes. It appears that proximal bracts subtending female flowers are less stimulated to lift and roll compared to distal bracts subtending female flowers and all bracts subtending male flowers. This was revealed by low vigour of bract lift and the small angle of lift at 08:00 h especially in the first female flower cluster (Figs. 2, 3). The bract angle of lift increases from proximal to distal end and this has been linked to reduced fertility in proximal clusters 2 . But it may not be the case since highly female (in all clusters) and male fertile 'Calcutta 4' showed the same pattern as edible bananas. The high R 2 for female bract roll scores compared to bracts subtending male flowers was a result of more bracts used to calculate averages for bracts subtending female flowers compared to bracts subtending male flowers (Fig. 3). For bracts subtending male flowers, two to three bracts were observed for most genotypes thus the first three data points were close to the trend line. Since the number of female clusters varies, reducing number of data points were used to calculate average bract lift angles in the distal end or larger inflorescences. Besides, bract lift angles of some clusters could not be measured because of obscurity or being in awkward positions. This led to the last two points being far off the trend line for angle of lift and hence a low R 2 .Flower opening time is said to be genetically and environmentally controlled, results from this study are in agreement since light had considerable influence on bract opening events (Tables 1, 3). Significant effects of temperature, solar radiation, and vapor pressure deficit on flower opening time have been observed in rice 11 . For Musa spp., only light has a significant relationship with BOT. However, there was early curling under long summer days in the glasshouse in Belgium compared to short days in Arusha field conditions 6 . This suggested a particular light signal for BOT in Musa spp. It is unclear why high light intensity led to early lift of bracts subtending male flowers and this calls for farther investigation. Since bracts subtending male flowers instinctively open later than bracts subtending female flowers, light intensity had less effect on the former bracts. The small sample size could have also had an impact on the results in the study, the light flush from the camera could have also affected the results. The extent of weather effects on BOT in banana need to be studied in field conditions of locations with significantly different day length for a more reliable conclusion.Hand pollinations in banana have to be done at the right time for maximum seed set which is critical for their improvement. This study assessed BOT to determine when flowers are likely to be most receptive. Banana bracts subtending female flowers start lifting late afternoon and complete opening mostly after midnight. This implies that current controlled pollination at 07:00 to 10:00 h might perhaps be too late. But it would make sense to try and pollinate as early as possible especially as soon as bracts subtending male flowers open. The aim should also be to pollinate when pollen viability is highest just after bract opening considering the fact that opening events time for bracts subtending female and male flowers may be different. Since bananas open bracts partly during day and partly at night, nocturnal and diurnal pollinators have a role to play as natural pollinators. Bract rolling and lift angles seem not to be linked to fertility as the highly fertile wild banana 'Calcutta 4' behaved just like the sterile edible bananas. For some genotypes like 'Gonja' , there is a considerable time lapse between female and male flower opening thus self-pollination is not a concern with aid of pollinators.","tokenCount":"4565"} \ No newline at end of file diff --git a/data/part_6/0602673022.json b/data/part_6/0602673022.json new file mode 100644 index 0000000000000000000000000000000000000000..81f6ec461554ff8b6f11f158bbac6d8cabb506d1 --- /dev/null +++ b/data/part_6/0602673022.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"4b5d8ccf828db1cfaa44b051502741bc","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/2fb697a8-0da5-468b-9e0d-29db8d586f4a/retrieve","id":"-173280691"},"keywords":[],"sieverID":"8e27ea9c-f1b4-407c-89ce-3c0a8a89874d","pagecount":"2","content":"resilience of local farming systems through sustainable land-use, and livestock, soil and natural resource management; boosting yields through continued genetic improvement, better agronomy, and pest and disease management; and increasing incomes by engaging smallholders more effectively with markets.By considering interactions of many different elements within and across the farm, landscape, value chain, and food systems levels, CIAT, in Asia, undertakes scientific research addressing questions and issues along the entire agricultural path, FROM SOIL TO PLATE.With more than 60 percent of Asian population either directly or indirectly relying on agriculture for livelihood, agriculture remains key to uplifting lives of many people in the region, as well as to providing sufficient and nutritious food for all.In Asia, CIAT undertakes scientific research enabling smallholder farmers, agri-food businesses, and national governments to use smart technologies and innovations and make evidence-based decisions, towards achieving profitability, environmental sustainability and resiliency in agriculture.Over decades, CIAT's research has directly contributed towards increasing competitiveness, efficiency and Planting highly productive, nutritious forages on small areas of the farm can allow farmers to increase livestock productivity without relying on increasingly scarce natural resources. CIAT's research aims to support farmers by making available forage options that meet quantity and quality requirements for profitable animal raising, while improving productivity through gains in overall efficiency and access to livelihoodenhancing ecosystem services.• Improved forage options for more productive and sustainable livestock production CIAT's research focuses on dynamic interaction between farms and landscapes, mobilizing climate science to facilitate informed decision-making on adapting to and mitigating climate change, including by protecting ecosystem services. Through the Climate Policy Hub, a portfolio of tools help guide land management planning and policy formulation by a whole range of stakeholders, from farmers to businesses to development organizations and governments. Some of these toolsclimate scenarios, impact modeling and assessments, vulnerability assessments, prioritization processes, policy analyses -also help identify, test and scale climatesmart agriculture technologies, while othersFor more information, get in touch with Dr. Peter Laderach at (p.laderach@cgiar.org).-climate advisory services, early warning systems, monitoring-reporting-verification (MRV) systems -aid implementation of climate change adaptation and mitigation measures.To help smallholder farmers tap into the abundant opportunities offered by cassava, CIAT continues to develop improved varieties and works to effect stable and sustainable yields through enhanced pest-disease, soil and seed system management. In order to enhance overall competitiveness of value chains, the team in Asia implements a mix of innovations, which includes investigating innovative options for adding value to cassava industrial waste, among others; using spatial analyses and geo-referencing techniques to track cassava demand and identify bottlenecks; investigating cassava seed systems, including policy and procedures for moving plant material; and analyzing regional policy, infrastructure and logistical regulations to evaluate supportive networks and value chainwide services which foster smallholder development.• Integrated, inclusive cassava value chains for diverse uses and markets• Stable and sustainable yields through enhanced pest-disease, soil and seed management system• Novel varieties for value addition and efficiency gainsResearch countries: Cambodia, China, Indonesia, Lao PDR, Myanmar, Philippines, Thailand, Vietnam For more information, get in touch with Dr. Dindo Campilan (d.campilan@cgiar.org).CIAT strengthens capacity of a wide range of agricultural value chain actors to engage with one another in a more inclusive and sustainable manner. Across Asia, CIAT supports Heifer International towards effectively including smallholder farmers and cooperatives into animal product value chains following the LINK methodology. Also being investigated is the effectiveness of Farmer Business Schools in Vietnam to enhance the capacity of vegetable farmers to engage effectively with markets.• More strategic public-private investment priorities in high-value agricultural commodities• Inclusive businesses through greater market participation of small-scale producers• Sustainable food systems for safe food and improved diet along the ruralurban transectFor more information, get in touch with Dr. Stef de Haan (s.dehaan@cgiar.org).By coordinating activities in Vietnam related to the CGIAR Research Program on Agriculture for Nutrition and Health, CIAT contributes to the goal of achieving healthier diets for poor and vulnerable populations through a better understanding of food system-diet dynamics, and through identifying and enabling innovations in value chains and policies. CIAT's work on food systems responds to concerns about diet trends, i.e. transitions, and demands, with a view to developing systemic solutions that address problems such as malnutrition and food insecurity. ","tokenCount":"694"} \ No newline at end of file diff --git a/data/part_6/0612615787.json b/data/part_6/0612615787.json new file mode 100644 index 0000000000000000000000000000000000000000..e3d6ad2e43b60cc3dc0d54bc9828494a50651ac8 --- /dev/null +++ b/data/part_6/0612615787.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"84eeed06ba027609e9ac79190fc1e246","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/b6761730-60e6-4576-befa-160562e96594/retrieve","id":"422242046"},"keywords":[],"sieverID":"b459840a-02d5-42d5-a435-7133b213df2b","pagecount":"89","content":"Con estos apuntes se pretende dar una visión general sobre la estadística y el uso de los Diseños experimentales en el cultivo de arroz.Inicialmente se da algunos elementos de esta-d~stica descriptiva y estad!stica inferencial y por última se describen COn ejemplos los diseños completamente al azar, bloques al azar, con un solo factor y verios factores y final.en_ te el diseño en parcelas divididas.Este pequeño manua: se pudo reali~ar por la colaboración btindada por los miembros de la Unidad de Servicios de Datos d_l • CIAT, por el Programa de Arroz, y las enseñanzas del ~Iofe~Qr 1 Jorge A. Escobar. ( por ej.: el número de macollas por sitio puede ser 15, 20, o el número de plantas de arroz por parcela puede ser 150, 160, etc. Otra clasificación de las variables es: Variables . , dependientes y variables independientes; estas últimas toman uno u otro valor dependiendo de los valores que tome la variable independiente, así por ejemplo. La producción de arroz (variable dependiente) depende de la cantidad de nitrógeno que se haya aplicado al suelo. 1. 3. Población física: Conjunto de individuos con características comunes, r' por ej.: Las plantas de arroz de la variedad CICA6., 1. 4. Población estadística: Conjunto de observaciones o mediciones de una población física, por ej.: Las alturas de las plantas de arroz. De una población física se pueden generar varias poblaciones estadísticas, así de las plantas de arroz, se pueden tener además de las alturas, los rendimientos de grano, las áreas foliares y otras. Huestra: Parte de una población física o estadística. Razones ~ las cuales ~ forman muestras. a) Porque es impos.ible o resulta antieconomico tomar a toda la población 1. 6.Para una mejor comprensi6n de los temas que se desarrollaran, se darán algunos conceptos básicos:1.1. Estadística:\"Es una ciencia pura y aplicada, que crea, desarrolla y aplica técnicas, que permitan colectar, analizar, interpretar datos, y tomar decisiones en casos de incertidumbre \". La estadística descriptiva, cama su nombre lo indica, solo sirve para describir poblaciones o muestras y la estadística inferencial permite tomar decisiones en casos de incertidumbre con una probabilidad de error.1 .2. Variable:Es una característica que varia, por ej .. CIERTO PARA LAS CIENCIAS AGRICOLAS y BIOLOGICAS\"-Ante la variabilidad de los fenómenos, la estadística proporciona herramientas ~ útiles, para poder tomar decisiones con niveles altos de confiabilidad, po r ej.: Se puede llegar a decidir si un sistema de siembra (transp~ante) en arroz, supera o no a los sistemas tradicionales, con un margen de probabilidad dado.Cuando se obtienen datos de una muestra o de una población física, es muy díficil sacar conclusiones, si el número de observaciones es muy grande, pudiéndose aplicar en 8Ste caso la frase \"Los arboles no dejan apreciar el bosque\", , .. \"-5-Tablas de frecuencia:Permiten agrupar las N observaciones de una muestra de acuerdo a su magnitud, en varias clases o grupos.Los pasos a seguir en la construcción de una tabla de frecuencia son:l.Seleccionar el número de clases (K); para esto puede utilizarse una regla práctica: . ' uso del criterio práctico se tomaron 12 clases (ver tabla 1). Nótese que la diferencia entre , t los limites de clase es igual para todas las clases 0.899 Ton/ha, lo cual es deseable cuando se construye una tabla de frecuencia.La Marca de Clase (MCi) se obtiene al promediar los l!mites de clase, y su funció~ es: Representar a todas las observaciones que caen dentro de una clawe i. l TABLA 1: Tabla de frecuencia, de la producción en toneladas por hectarea del \"Vivero internacional de rendimiento de arroz para AmericaLatina\" -Variedades Tempranas. La media ponderada X p es:Siendo fi, el factor de ponderacion, en este caso el número de Has. o sea:En las tablas de frecuencias, se halla la media, ponderando las marcas de clase por la frecuencia absoluta.Por ej.: para hallar el promedio de las producciones del VIRAL-Tempranas (ver tabla 1) se procede así:.07 ton/ha.Las medidas más conocidas son:-Rango (R)Desviación están--Coeficiente de Variación (CV)El Rango (R) es la diferencia entre el mayor y el menor valor de un conjunto de datos, así el Rango de las observaciones de la Pago 10 es: 7.0-1.0=6.este caso Ton/ha.La Varianza (S2) se halla así: x 100Este parámetro no tiene unidades y permite:Comparar la variabilidad ue características igua-les en poblaciones diferentes. Por ej,: Rendimiento de grano de arroz vs. rendimiento de grano de fríjol.Comparar la variabilidad de características diferentes en poblaciones iguales, asi por ej.: el rendimiento de grano en arroz vs. altura de plantaso En las tablas de frecuencia se puede calcular la va- que provienen de muestras con diferente n6mer6 d~ observaciones.La fórmula es:Siendo S2. = Varianza de la muestra i1.GLi = Grados de libertad asociados a la varianza i (ni-1)En el siguiente ejemplo, en el cual se tienen las varianzas de la producción de una variedad en (ton/ ha)2, provenientes de experimentos con diferente n6- Al probar este tipo de hipótesis de nulidad pueden En la Tabla 1 • se calcula:-Qu~ % de los Rendimientos fueron menores de 7 • 2 ton/ha -Qu~ % de los Rendimientos fueron mayores de 9 .9 ton/ha. (,CORRELACION y REGRESION:*En esta parte se pretende dar una idea general sobre la correlación y la regresión, sin entrar a detalles matematicos; este tema sería tratado con mayor profundidad al.final de este curso.Es el grado de asociación que existe entre dos variables.Existe correlación positiva cuando al incrementarse la magnitud de un« variable, la otra también se i ,crementa, así por ejemplo al incrementarse hasta cierto límite la cantidad de Nitrógeno, la producción de arroz aumenta, o al aumentarse el número de desyerbas la producción aumenta.Existe correlación negativa, cuando al aumentarse la magnitud de una variable, la magnitud de la otra disminuye, as! por ejemplo, al aumentar el peso de malezas por unidad de area, disminuye el rendimiento de grano.Cuando se asocian dos variables, generalmente a una se le denomina variable independiente y a la otra variable dependiente; los valores que toma esta última dependen de los valores que ha tomado la variable dependiente, así por ejemplo, la producción de Capítulo tomado de MuRoz, J.E. \"Curso de Arroz para profesionales de America Latina\"~ Primer curso de 1979.producción depende de la cantidad de malezas presentes.Como cuantificar el grado de asociación:Al realizar un diagrama de dispersión, en el cual se ubica en el eje X la variable independiente y en el eje Y la variable dependiente, y localizar los respectivos pares de datos. se puede apreciar si existe o no correlación 7 si esta es positiva o negativa; pero no se puede determinar ningun valor.( Para cuantificar el grado de asociación se usa el coeficiente de correlación = r, cuyos valores están entre menos uno y más uno así:En la medida en que el coeficiente de correlación se acerque a uno, en valor absoluto, la asociación tiende a ser perfecta, cuando el y es positivo hay correlación positiva y cuando r es negativo hay correlación negativa.La fórmula para calcular res:• .. .1. Tabla 2:\"Efecto de la competencia de malezas en arroz\".Rendimientos de arroz en Kg/parcela y peso de malezas en Aleatorización o asignación al azar de los tratamientos.Control del error experimental.La aleatorización evita la introducción de errores sistematicos en los experimentos, es decir evita que unos tratamientas se vean mas favorecidos que otros. Así,si una variedad de arroz se siembra en un terreno fertil y la otra en un terreno con problemas de fertilidad, no puede concluirse que una variedad produce mas que otra, porque no se puede saber, si la producción se debe a la variedad en si misma o a la fertilidad del suelo.La repetición es el número de veces que un tratamiento se repite en un experimen~o, es deseable que el número de repeticiones sea igual para todos los tratamientos para compararlos con el mismo nivel de precisi6n.El error experimental se puede minimizar controlando las Es muy importante seleccionar con anticipación las variables de respuesta que van a ser cuantificadas.Asi en ensayos de rendimiento, se mide la producción;en ensayos para control de malezas, ademas de la producción de arroz, debe tomarse una medida sobre el grado de control de malezas por ejemplo: ?eso seco de malezas.(DISENo COMPLETAMENTE AL AZAR:6.1.Características Generales:Es el diseño más simple, y se usa cuando las unidades experimentales son homogéneas.Se puede comparar cualquier numero de tratamientos.Los tratamientos se aplican a las unidades experimentales al azar.Cualquier numero de repeticiones por tratamiento es posible, es decir los tratamientos pueden tener diferente número de repeticiones.Proporciona más grado de libertad al error que cualquier otro diseño, con el mismo número de tratamientos y repeticiones.Dada la restricción de que las unidades experimentales deben ser homogéneas, se usa muy poco en el campo, pero es de mucha utilidad en experimentos de laboratorio o de invernadero, donde se pueden uniformizar las unidades experimentales, por ejemplo en invernadero el suelo se puede homogenizar, y en el laboratorio se puede preparar un medio de cultivo homogeneo.Modelo Estad!stico:Este diseño experimental está asociado al siguiente modelo estadístico:Error experimental en la repetición j'esima del tratamiento i'esimoEfecto de la media general Variable de respuesta ¿el tratamiento i'esimo, en la repetición j'esima.Con los siguientes supuestos: -47pletamente al azar, como se observa en el siguiente esquema: .SCT = i=l Se halla la suma de Cuadrados Total (SCT) Sehabia lanzado la hipótesis nula de no hay efecto de trata--50-La regla de decisión para probar la hipótesis es:Acepto H : Si Fe < F t F t se busca en la tabla de F. con un nivel de significancia dado, en nuestro casO 5%, con los grados de libertad de tratamientos y del error así:-51-7.-52-DISENo EN~BLOQUES COMPLETOS AL AZAR: ---------------------------------------------------------------------------------Porque de esta manera habra mas homogeneidad dentro del bloque, y más diferencia entre bloques. En este caso el largo de las parcelas debe ser paralelo al gradiente de fertilidad.Otro caso en el que se puedan usar bloques rectan-gulares, ocurre cuando existe alguna pendiente en el terreno, ya que en la parte \"baja\" del lote habrá mayor deposición de materiales provenientes de la parte alta y por esta razón el suelo puede ser -55-Cuando se quiere por ejemplo, probar el efecto dé algunOS tratamientos sobre el control de las malezas, se pueden detectar por reconocimiento del terreno zonas de alta, mediante y baja infestación de malezss, al \"bloque\" con forma geometrica definida deja ~e ser útil y se convierte en un conjunto de parcelas que tienen como característica común el grado de infestación de malezas así:1;;; ::;..:;-.-,././v ,yrr\"'\"\". \" -,..., , i) Sea:Media del bloque J Entonces:'V -' . 'Jo. es un estimador de j.l , Yi' es un estimador de II + ' j Y.j es un es.timador de j.l + 8 j Ro: Sj = O para todo j (no hay diferencia entre bloques).Las reglas de decisión para las 2 anteriores hipótesis son similares a las reglas de decicion para el diseño completamente al azar.La construccion de la tabla de análisis de varianza, se puede resumir asr:( ..7.5. Ejemplo numérico de un diseño en bloques al azar:\" \" Titulo: \"Ensayo de rendimiento para cuatro lineas de arroz y una variedad testigo\".En la tabla siguiente se presentan los rendimientos por parcela. expresados en tone lad as por hectárea. En base a los grados de libertad y a la 'suma de cuadrados se obtiene la tabla de análisis de varianza de: Como se observa en la tabla de análisis de varianza, se puede dividir la suma de cuadrados de tratamientos, y se prueban aisladamente cada uno de los efectos, encontrándose que rechazó la hipótesis nula Realmente no es un dise60 experimental, sino un \"arreglo de tratamientos\".9.1. Características:Las características principales son:Se usa solamente para experimentos factoriales, generalmente cuando se estudian dos factores.Se utilizan dos tamaños de parcela, la de tamaño mayor se denomina parcela principal y la parcela de tamaño menor subparcela.La aleatorización se cumple en dos etapas, en la prim~ra se asignan al azar las parcelas princi-, .pales y en la segunda se asignan al azar las subparcelas en las parcelas principales.Se compara con mayor precisión el factor que se asigna en las subparcelas.Es ventajoso cuando por alguna razón práctica, un factor debe tener parcelas grandes, o cuando se desea comparar con mayor precisión un factor que otro.En la mayoría de los casos, este arreglo, se realiza bajo un dise60 en bloques al azar, razón por la cual, en la aleatorización y en el modelo estadístico se tendri en cuenta el efecto de \"Bloque\".('\" ","tokenCount":"2055"} \ No newline at end of file diff --git a/data/part_6/0638233476.json b/data/part_6/0638233476.json new file mode 100644 index 0000000000000000000000000000000000000000..2e58fc556ced9fae9235cd1d45c904c0045e87a7 --- /dev/null +++ b/data/part_6/0638233476.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"36d9f65e3e895ba82416afafe6dccfd8","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/f4df7fc7-2eae-4e0f-9a77-edddb6388ca0/retrieve","id":"-63315342"},"keywords":[],"sieverID":"687fdd64-c121-4cb8-abda-661ad6282445","pagecount":"121","content":"The crop Cassava (Manihot escuienta Crantz) is a starchy root crop that has been cultivated in tropical America for more than 5,000 years. It was introduced to Africa and Asia by Portuguese explorers and traders during the 16 century. Cassava represents the alimentary basis for over 500 million people in the tropics, and it is grown in over 90 countries. Most of the crop is produced by small-scale poor farmers in marginal regions using traditional farming methods. The crop is used both as food and feed, and it represents one of the few linkages for farmers in those regions to dynamic markets.Cassava is grown over 16 million hectares, with 50% in Africa; 30% in Asia and 20% in Latín America. Total root production is over 150 million; the five major producing coumries being: Brazil (24 million tons, Mt), Nigeria (21 Mt), Zaire (20 Mt), Thailand ( 19 Mt) and Indonesia (15 Mt). The greatest consumption of cassava is in Africa, with an average 88 kg per capita (highest in Zaire with 387 kg per capita). Ofthe total world production, 80% is used domestically (61% food; 26% feed ; 2% industrial uses and 11% waste). Cassava for feed is exported as chips, pellets or starch to developed countries. Cassava is mainly processed on a small scale in rural areas, generating considerable employment, and improving the economic status of socially depressed areas in the tropics.The cassava root contains between 30 and 40% dry matter (mostly carbohydrates); it is rich in vitamin C, Ca and K, and poor in proteins. In contrast, the leaves contain high levels ofprotein (8%-l O% of fresh weight). Cassava roots are eaten in different ways: boi led, baked, fried, as meal, flour, etc. Starch produced from cassava roots is al so used to make a variety of sweet and savory foods such as crackers, tapioca pearls, noodles, or cheese breads. In sorne parts of the world, cassava leaves are consumed as a vegetable.Cassava is known to produce well despite marginal conditions of soil and climate, dueto its extensive fibrous root system, the sensitivity of stomata to drier climates, its association with so il mychorrhizae and the capacity to recover foliage once it is lost. Cassava can be grown in environmems that receíve less than 600 mm to more than 3,000 mm/year of rain . Although cassava is typically a tropical lowland crop, it can be found up to 2000 masl. It does not tolerate frost or flooding well. The crop can be harvested from 7 months to 2 years after planting. It stores relatively well underground, and once it is harvested it has a very short shelf-life (3-7 days), and must be consumed or processed immediately.Cassava is vegetatively propagated through stem cuttings; which allows to fix any gene combination that suits the farmer or the breeder. The most important source of genetic variability is through sexual recombination. Male and female flower s are separated in the same inflorescence (monoic), being a protogenic species. Each pollinated flower has the potential to produce 3 mature seeds. Natural pollination is done by bees.. .Although most ofthe genetic variability used in breeding prograrns comes from the cultivated cassava, there are more than 100 species within the genus Manihot, that can cross to cassava with different degrees of difficulties and contri bu te genes of interest to breeders.CIAT has a collection of close to 6000 cassava accessions formed by landraces from Latin America and Asia, elite clones selected by CIAT and liTA, and 29 wild Manihot species. This collection is maintained in the field and in vitro, and has been characterized using morphological, bíochemical and molecular descriptors.Sources of resistance or tolerance to majar pests, di se ases and abiotic stresses:• Genotypes combining high levels of resistance to bacterial blight and super elongation disease, with good agronomic performance selected in Eastem Colombia. • Complementary sources of resistance to different bacteria! blight pathotypes selected.• Genotypes with high levels of resistance to different pathogens causing root rots ha ve been selected, and are being used in crosses to pyramid those genes and for molecular mapping. • Highly heritable resistance to whiteflies has been detected and is being combined with desirable agronomic traits. • Reduced levels of whitefly oviposition is one of the resistance components consistently transferred to the progenies.• A RAP.O marker has been associated to whitefly resistance, and will aid in future breeding.• Sources of resistance to green mites, which are stable across evaluation sites ha ve been found .• Cassava roots and lea ves ha ve been characterized as sources of micro-nutrients for marginal regions of the tropics. There is good potential for vitamins A and C in the roots and minerals in the leaves. • Genotypes with prolongued storability have been incorporated in crosses for future breeding and molecular mapping. • Cassava starch can be used in processes that require resistance to acid media and freezing.Cassava starch can compete well with maize starch in food and industrial processes.Gene poo/s for lowland semi-arid, sub-hum id, hum id, mid-a/titude, high/and tropics and subtropics:• Between 1996-98 a total of458,027 recombinant seeds were produced; 253,148 were distributed to National Programs in Latín America and Asia and IIT A, the rest was used in our breeding process, or kept in storage. • An average superiority of 68 % of the best selected parents over check varieties was achieved across ecosystems during the last 3 years. Largest improvement obtained for the highland tropics ecosystem, followed by mid-altitude tropics and the acid soil savannas. • The significan! genetic improvement in cassava yielding ability at Rayong FCRC (Thailand) is due firstly to the enhanced biomass and secondly to the improved harvest index.lmproved breeding methodologies, includingfarmer participatory approaches:• Gene ta ing to facilitate future cassava breeding is on its way for the following traits: ~hitefly C!_nE roo~ rot r~sist~~ce, and reduced post harvest deterioration, using the molecular map developed at CIAT.--•---• Farmer participatory evaluation of advanced selection is an integral part of the cassava germplasm development schemes implemented in Northem Colombia and North-East Brazil. • 60 technicians in Brazil and Colombia have been trained in participatory breeding.• A software for the analysis of farmer participatory breeding data has been developed and made available to National Programs.Networks and trained national personnelfor effective dissemination of gene tic material:• Latin American and Asian networks of scientists ha ve been supported through the organization ofworkshops, local training and supply of improved germplasm. • 14 new var ieties released within the Latín American and Asían network, during 1995-98.• Improved varieties developed from CIAT's germplasm cover about 1,000,000 has in Latin America and Asia. • Benefits derived from improved CIAT's germplasm have accumulated to U$ 800 millions across continents, since the inception of our breeding program.The major goal of our project is to contribute in increasing and stabilizing cassava production in diverse environments and for different markets, by developing improved gene pools in cooperation with national programs. The purpose of our project is to generate basic understanding, tools and improved cassava germplasm for sustainable enhancement of cassava production and the diversification of end-uses in relevant ecosystems. The most important ecosystems are: the semi-arid (below 800 mm/year, unimodal); the sub-humid (800-1500 mm /year, bimodal rainfall distribution), the acid soil savannas ( 1500 -3000 mm/year, short dry period, low pH). The humid tropical lowlands; the mid-altitude tropics; the high-altitude tropics and the subtropics represent ecosystems of secondary importance in terms of area and total production. The main selection acti vity is conducted in sites selected to represent the conditions of the target ecosystem. F or each of the zones we conduct a recurrent selection program, with a progressive set of stages as described in Figure l .As the breeding stages progress, we give emphasis to traits of lower heritability, because we have more planting material for each genotype, and the evaluation can be conducted in bigger plots with replications. Certain selection criteria are of general importance across ecosystem (i.e. yield potential, d tt. er content), while others are specific for each ecosystem (i.e. pest and ats\"eáses . E 1te genotypes are used as parents to obtain recombinant seed that is used to initiate a new selection cycle, and also transferred to National Programs for their adaptive selection programs. Besides the selection of superior genotypes a major research priority is the development and use of research tools that will shorten the breeding cycle and increase its efficiency, such as molecular marker _assis~ec!_ selecti.Qn, and farmer participatory evaluation at early stages of the breeding cycle. New sources of resistan ce to major biótic a nd abioiic constraints, and favorable alleles for root quality traits are constantly being incorporated through recombi nation and selection. Basic cassava breeding scheme applied for each of the priority ecosystems. Collaboration with Nationai Programs: CIAT has been actively involved with CORPOICA and CNPMF/EMBRAP A in the development and implementation of methodologies for the evaluation and selection of cassava germplasm with the participation of end-users. Also with CNPMFIEMBRAP A, a program has been developed for the development of cassava germplasm with adaptation to semi-arid conditions, and potential to be transferred to homologous conditions in Africa and Asia. In Thailand, CIA T works with FCRI, on the development and diffusion of improved cultivars that have imp~ed and diversified-the genetic base for cassava production in the region. This work has had a tremendous impact on cassava production in Asia with wide dissemination of improved varieties. CIAT and liT A ha ve active! y collaborated in the development and introduction of germplasm into Africa, combining elite Latín American genotypes with sources ofresistance to African Cassava Mosaic Disease. Recently, in Latín America, an active interaction with the prívate sector involved in starch and feed production has been initiated, to -learn more about their demands and tap new sources of financia! support.In Asia and Latín America and the Caribbean, there are relatively few strong cassava breeding programs. Among them are El\\IIBRAP A/CNPMF (Brazil), INIVIT (Cuba), CORPOICA (Colombia), FCRI (Thailand), and lAS (Vietnam).Our project has centered its activities around the consecution of the following three outputs:l . Genetic base of cassava and Manihot species evaluated and available for genetic improvement. 2. Genetic stocks and improved gene pools developed and transferred to National Programs and liTA. 3. National Programs in tropical and sub-tropical Latín America and Asia supported in adaptive selection and deployment of improved cassava varieties.The main activities developed during the last 3 years, along with the main results are:l . Genetic base of cassava and Manihot species evaluated and available for genetic improvement.l. l. Germplasm characterized for reaction to cassava bacteria! blight (CBB); super-elongation disease (SED) and stem and root rot diseases (RR), resistance under field and reenhouse conditions • Evaluate cassava genotypes for CBB and 1254 genotypes evaluated for CBB and SED reaction. 526 in SED reaction in Villavicencio and Matazul Carimagua, 483 in Villavicencio and 245 in both sites. 62 clones found tolerant in Villavicencio and 7 1 in Carimagua: 3 7 clones were tolerant in both si tes, 5 of with 5 cycles of evaluation.• Evaluate cassava genotypes for RR reaction in the greenhouse • Evaluate 60 genotypes with different pathotypes of CBB causal agent.• Develop an efficient inoculation method to select fo r res1stance to RR Phytophthora spp.• On-farm evaluation of prornising genotypes in regions of Colombia where RR are endemic (Mitú. Santander de Quilichao)• Evaluate parental material. segregating and mapping progenies fro m contrasting genotypes under greenhouse conditions.16 out of 430 genotypes selected as resistant to Phytophthora drechsleri by stem inoculation of young plantlets in the greenhouse. Ten of them also have resistance to Phytophthora parasítica and Phytophthora cryptogea. 30 genotypes resistant to P. drechslerí by evaluation of roots fro m a group of 150 .103 genotypes evaluated. lO had intermedia te reaction to 10-1 2 strains of Xanthomonas axonopodis pv. manihotis.Two root and stem inoculation techniques under greenhouse conditions for severa! Phytophthora species were developed and with hi gh correlation with field resistance of cultivars.Mitú: 9 promising root rot resistant genotypes with different cyanide content were planted in 3 indigenous communities. to be compared with local varieties. lO varieties wcre planted in two govemmemal farms at Mitú. Santander de Quilichao: 16 promising genotypes with resistance to RR planted in a naturally infested field with high incidence of Phytophthora . Local varieties were included as control.Prcliminary results indicate h..ighly significant differences between TSM 30572 (MNGA 2) and CM 2 177-2 by inoculation of the stems of young plantlets in the greenhouse with P. drechs/eri . Participatory farmer evaluation of advanced genotypes as a routine procedure within the breeding scheme. A group of varieties preferred by farmers is Wlder multiplication and proposed for formal release. Germplasm transferred to Sub-Sahelian Africa.Genotypes selected with a 34% higher dry matter production potential than check varieties. A network of representative si tes fo r tesung genotypes in Northen Colombia has been established.Pote ntial of cassava to compete with more industrial crops has been determined. Thirteen genotypes have been selected as the basis for next-year on-farm trials.Integrated research and developmcnt proposals developed a nd submitted to donors. Clase interaction with the National Programs established.A network of companics involved in feed a nd food industry devcloped for testing and multiplication of cassava germplasm.Contact made with institutions from the prívate sector (related to cassava processing). The idea is supportcd by public programs.A de-ccntralized seed multiplication program established in Northe m Colombia. 1:800:000 high quali ty stakes produced fo r regional and experimental genotypes. Techmcians involved with multiplication plans were trained.Summary of resu lts 3.3 Develo ment andada tation of end-user • Support fanner participatory germplasm evaluation in NE and Southern Brazil.A network of ~O extension agents and scientist has been t:rained in the methodology and are active! y working. Manuals have been developed.• Work together with NGO's in Northem Cauca for the evaluation of improved cassava germplasm with farmers and processors • Start project on fanner participatory evaluation of early breeding cycles in Northem Colombia• Publish Cassava Participatory Breeding manual en English.The way ahead A group of 18 technicians from the public sector and NGO's trained on farmer participatory evaluation.Financia! support approved to stan working in October 1998.Multiplication of genotypes to incorporare in early on-farm evaluation.Translation in process. Data analysis package has been produced (diskette and manual are available).2 papers have been published in Brazil. and 2 more are in preparation for pÜblicatlon in inremarionaJ joumals.We will continue with the stream-line approach of: screening the available genetic base for useful genetic information, combine complementary sources into improved gene pools, and transfer those gene pools for adaptive selection with National Programs. We foresee a greater use oftools that will help us improved the efficiency of cassava germplasm development.Finalize the molecular map: The actual molecular map of cassava was developed on a progeny of 90 individuals, work is o n-going to complement the map through the extension to 150 recombinant progenies, necessary for studying quantitative traits. Back cross progenies from selected individuals in the mapping populations will be u sed to developed a B 1 C 1 map by transfer of framework. The project is active\\ y involved together with CIA T' s project SB-2 in activities aimed at obtaining a highly saturated map, that will serve as the basis for tagging genes responsible fo r important processes, and implementing MM assisted selection.Development and application of advanced tools for molecular characterization : An effective implementation of MMAS and gene tagging will depend o n our ability to adapt and implement the most effective tools for molecular characterization, as well as probes related to biochemical process of prime importance in cassava. The major activities in the near future will be the selection and implementation of multi lex mi~ro satellüe analy?is _é!nd the acquisition and preparation of genes for important metabolic processes (i .e post harvest deterioration). lt is planned to develop additional micro satellites and DNA markers for cassava germplasm characterization, including probes from genes involved in important metabolic processes.Implement molecular marker assisted selection: One of the majar objectives of adjusting molecular characterization techniques will be to incorporate them as one of the breeders' tools for more effective genetic enhancement. The obvious traits for which MMAS will be applied at the beginning are those for which we do not ha ve the proper screening environment (i. e. ACMV resistance), and those for which there is a screening procedure, but it is subjected to environmental influences and variable pressure from biotic sources (i.e. whitefly resistance, root rot), or it is too costly and restricted to a small number of genotypes at a time (i.e. starch amylose/amylopectin).Once MMAS is preved effective within the developed genetic stocks and the proper parental material is identified, it should be incorporated into the progenies that are the basis for the recurrent selection program, and fully implement MMAS as a breeder tool.Identify and study sources and mechanisms of useful genetic variability in cassava and Manihot species: Priority will be given to screening the core collection, and wild species collection as sources of useful genetic diversity. Once desirable traits are identified, mechanisms and processes responsible for the higher levels of expression of those traits will be studied in order to comprehend and manipulate them in a more effective way. The active input from Pathologists andEntomologists is expected to continue. The main gaps to fill will be in the areas ofPhysiology and Root Quality. There will be a need to search for altemative ways of keeping competence in these are as.Agronomic evaluation of mapping populations and genetic stocks: Interaction with other scientists will be crucial to set priority traits for gene tagging molecular assisted selection. A meaningful agronornic evaluation depends on the sites we can use for that purpose. The 3 main sites used for the recurrent selection program, may not be able to suit all the evaluation purposes in the best way. Field evaluation in additional sites is an expensive endeavor, therefore we should príoritize these activities in order to come up with the most effective way for testing. Molecular and phenotypic information will be integrated using the genetic map to localize important genes for each trait in • preparation for MMAS.Development and maintenance of genetic stocks: Populations are developed by the recombination of contrasting genotypes for a particular trait (i.e. whytefly resistance) or sets of traits (physiological traits). The majar purpose of these stocks is to provide the basic material for heritability studies, gene tagging and studying of mechanisms of resistanceltolerance, efficiency, etc.Incorporation of wild germplasm into cassava: Although we have limited information on the potential of Manihot species as contributors of valuable genetic information, there are already a few well documented cases that will deserve our attention in terms of inter-specific crosses, and making that genetic information available to the breeding programs; escaping drought (Manihot spp, \"manicobas\"); leaf anatomical structure (Manihot rubricau/is); and waxy starch (Manihot crassisepala). Once we know more about the potential contribution of wild species for quantitative traits, a QTL breeding program can be initiated using a very broad base population.Development of parental populations: The recombination of selected genotypes from the recurrent selection scheme, will not only provided initial material for the following selection cycle, but it will generate the populations that will be supplied to NARs. The recombination work should be expanded to incorporate a larger proportion of crosses between CIA T' s selected genotypes and genotypes of interest to NARs (i.e. landraces from Asia, ACMV resistant genotypes from liT A, etc.).Implementation of a recurrent selection program across ecosystems:Improved germplasm continuous to be the core of the project activity, and the most important link to National Programs. Presently, improved gene pools are developed for 6 of the 7 previously defined ecosystems where cassava bears importance: Sub-humid lowland tropics, Semi-arid lowland tropics, Acid soil savannas, Mid-altitude and Highland tropics and Sub-tropics. In the 1998-200 l MTP reduced emphasis was assigned to breeding activities, with higher emphasis given to pre-breeding work. For this reason we propose to concentrate on the development of a low-land tropics improved populations through a recurrent selection process involving 3 sites: Santo Tomás (Sub-humid), Santander de Quilichao (Mid-altitude and acid soils); and Palmira (more fertile soils). The proposed scheme is based on a more systematic evaluation of a much reduced number of initial segregating progenies. Selected genotypes will be used to generate recombinant progenies to re-start the selection cycle, and they will al so be crossed to sources of local adaptation (i.e. Asian landraces, ACMV resistan! lines from IlT A, etc.) to generate at least 50,000 seeds/year intended for distribution to liTA and NARs. A similar scheme will be put in place for the midaltitude and highland tropics ecosystems combined. Germplasm development for the semi-arid is also considered in the special project financed by IF AD until 1998.Distribution of improved populations and/or genetic stocks to NARs and AROs: This constitutes the nexus of our institution to other programs in the developing world . Currently we are producing more than l 00,000. seéds/year for distribution. It is foreseen that the number will shrink to 50,000 in the future due to a reduction in our breeding activities. Population development and seed distribution will be rationalized, given the enhanced capacity of sorne National Programs to produce their own segregating material.Relationship with National Programs in Latin America: This is the only outreach activity for Latin America, and it is based on a 3 -year special project financed by IF AD . This project is conducted in close collaboration with our Brazilian counterparts. and includes germplasm evaluation, selection and recombination, as well as farmer participatory evaluation of advanced selections; and supply of segregating progenies to other programs in homologous environments (i .e. liT A for semi-arid Africa). There is considerable interest from the prívate sector (mainly processors) to support cassava research (particularly the development and diffusion of new varieties). CIA T is working to build a Latin American consortium for cassava research . This will not only contribute research funds, but also bring new opportunities for the participation of end users in designing and executing research.The majar goal of our project is to contri bu te in increasing and stabilizing cassava production in diverse environments and for different markets, by developing improved gene pools in cooperation with national programs. The purpose of our project is to generate basic understanding, tools and irnproved cassava germplasm for sustainable enhancement of cassava production and the diversification of end-uses in relevant ecosystems. The most important ecosystems are: the semi-arid (below 800 mrnlyear, unimodal); the sub-humid (800-1500 mm /year, birnodal rainfall distribution), the acid soil savannas (1500-3000 mrnlyear, short dry period. low pH). The humid tropicallowlands; the mid-altitude tropics; the high-altitude tropics and the subtropics represent ecosystems of secondary importance in terms of area and total production. The main selection activity is conducted in sites selected to represent the co nditions of the target ecosystem. For each of the zones we conducta recurrent selection program. with a progressive set of stages.As the breeding stages progress. we give emphasis to traits of lower heritability, because we ha ve more planting material for each genotype. and the evaluation can be conducted in bigger plots with replications. Certain selection criteria are of general importance across ecosystem (i.e. yie ld potencial. dry matter content). while others are specific fo r each ecosystem (i.e. pest and diseases). Elite genotypes are used as parents to obtain recombinant seed that is used to initiate a new se lection cyc le. and also transferred to National Programs for their adaptive selection programs. Besides the selection of superior genotypes a majar research priority is the development and use of researc h tools that will s horten the breeding cycle and increase its efficiency. such as mo lecular marker assisted selection, and farmer participatory evaluatio n at early stages of the breeding cycle. New sources of resistance to majar biotic and abiotic constraints. and favorab le alleles for root quality traits are constantly being incorporated through recombination and se lection.CIAT has been actively involved with CORPOICA and CNPMF/EMBRAPA in the development and implementation of methodologies for the evaluation and selection of cassava germp lasm with the participation of end-users. Also with CNPMF/EMBRAP A. a project has been implemented for the development of cassava germplasm with adaptation to semi-arid conditions. In Thailand. CIAT works with FCRI. on the development and diffusion of improved cu ltivars that have diversified the genetic base for cassava production in the region. This work has had a tremendous impact on cassava production in Asia. CIAT and liTA have active ly collaborated in the development and introduction of germplasm into Africa. combining elite Latin American genotypes with sources of resistance to African Cassava Mosaic Disease. Recentl y. in Latín America. an active interaction with the privare sector involved in starch and feed production has been initiated. In Asia and Latín America and the Caribbean. there are relatively few stro ng cassava breeding programs. Among them are EMBRAPA/CNPMF (Brazil). INIVIT (Cuba). CORPOICA (Co lombia). FCRI (Thailand). and lAS (Vietnam).This repott summarizes activities and resu lts obtained during the last 3 years. with greater emphasis nn the activities deve lo ped during the period 1997-98. • Rapid multiplic< ttion of promising genotypes for future ex perimems• Develop an eflicient inoculation method to selecl tor resist< mce to Plrywplalwm spp.• On-lann evt.~luation of promising genolypes in regions of Colomhia where RR < Ue endemic (Swuander de Quilíchao, Mitü)• Evaluate parental material r resistw1ce to sternhorer C. c/arkei• Pl;ulling of :.clected germplasm for pest resist high as 105 mg/ 100 gr. In userage. cassava lea ves can ha ve 30 times the carntene cnncentratinn fo und in the roots of yellow cassava. Se lecting the genotypes with the highest cnncentratiun of carotenes in the lea ves will allow to suppl y the daily requirements (between 3 and 4 mg) of vitamin A for an average person. with just 5 grams of fresh lea ves or 2 grams of dry leaf t1our. Results showeú that experimental erTor• can be reduced to reason<1ble levels (F fin ponleú samples acrnss plants and reps. still significant) by sampling 2 plant'i per replication and pooling at least 2 rnots per plant (previously washed and peeled) and 10 developed leaves from the upper third of the plant. This stud y also showed a high positive cotTelation between mineral co ncentration in roots and leaves nf the same genntype.s for Zn and Mn: w hile for Ca the cotTelatinn was highly negati,•e. For other minerals. variability in concentration was independent in rnnts and lea ves.Applying the previously llescribed samp ling pmcedure. a set nf 20 genotypes planted in -f. reps was sampled at 6 momh after planting ro e,•aluate the co ncentration of minerals in both leaves and roots (Data not shownl. The coetlicient of variation (CY9'c) was high fm the cnncen[ The shnn posr-harvest storage life of cassava roots is an intrins ic charucte ristic that affects the marketability of the product. Ronts ha ve to be co nsumed nr processed slmrtly a.fter har\\'est. Post-harvest physio log ical deterioration (PPO) beg ins as earl y as 24 huurs nf han•est. resulti ng in ront prod uction and 4uality losses. high marketing margins and risks. and restricted manage me nt tlex ibility for farmers. traders and processors.A limited amount nf genetic variability fo r PPD is available in cassava germplasm. providing 4uantitati\\'e ge notypic ditlerences in the shel f-life nf cassava betwee n very few days to one nr possibly two weeks. Hmveve r. selectinn fm reduced PPD from ex isting ge netic variants in breeu ing prngrams is se rio usly hampered by e nvironmental effects and the complex inherita nce nf se,•e ral tra its unc.J er s imu ltaneous impmve ment. Ü)mmon ly cul ti vatec.J cassava varieties ha ve shelf li,•es nf nnl y a few c. Jays. Although res ults fro m early research showed a e lose positive aSS\\lciatin n be t\\veen PPD and rno t dry matter come nt. recent results fro m studies o n a wic.Je r ,gene tic range. failec.J to finc.J a significant assoc iation betwee n those traits.The process nf PP D in cassava rese mbles wounc.J-healing responses found in other plant systems. Such t.lefensive responses are eventua ll y inhibitec.J by successful wounll repair in the majority of the syste ms stud ied. However. this repair process is not successful in the harvested cassa va storage root (except under certain storage conditions as described be low). leading to the hypo rhes is rhar um•estrained cascades of wo und responses ultimate ly result in root deterioration.The objective of the present work is to associate PPD resistance/susce ptib ility reactions to other root tra.its of accumulation of metabo lites in the roots. in order to fac ilitare germp lasm scree ning. gene ide ntiíication and tagging. as we ll as increasing the effic iency of the breeding wo rk.The wo rk was deve loped at CIAT (Palmira) during the period September 1995 -March 1 9n. Two screening trials were established with 16 and 63 e lite ge notypes with 3 replicat io ns in plots of 25 pla nts. A random sample of 5 roots fro m each geno type was harvested and eva luated accn rd ing to the procedure described by Wheatley ( 1982). Distal and proximal extre mes nf each root were cut in order to obtain a 15-cm piece. The distal pan of the roo t was cnvered with a plastic fi lm and secured with a rubber band. Ronts we re sto red in a coo l shatled place and were evaluated at 5. 1 O and 15 days after harvest. Each roo t was cut into se ve n 2-cm ,;! ices . and eac h si ice was scored for PPD. Re lated root traits such as dry matter content (9é ) and cyanide were reco rded in both trials. and starch and sugar co ntent was measured in the first tria !.In the screen ing of 1 ó ge notypes run during the crop cycles 1995-96. PP D at 1 O and 15 days was co rrelated with starc h cooking fac ility (FCC). starch instability (INE) and the ratio nf total sugars ( AZ) to starch conce ntration (AL) (Table 1.3.2.1 ). Pri ncipal component analysis cn nsitlering those traits as intlepentlent variables revealetl that the ratio A7J AL had the largest pnsiti ve re latinnship with PPD. The second largest co mpo nent. but having a negat ive etf ect nn PPD was starch coo king fac ility. whic h represents an intrinsic c haracteristic nf cassava starch. T he evaluatio n of the same genotypes during the cro p cycle !996-97 resulred in a sig ni ficant difference amo ng ge notypes at 5 and 10 DAH. but no differe nce was observed at 15 DAH. Fm m this tria!. 3 ge notypes with less than 20% PPD at 15 tlays were selected tn be used in crosses for builtl ing up Ta ble 1.3.2. 1. PPD reacti on nf elite germpla.sm and re late<. ! roo t and starch charac teristics. We. ha\\•~ recently started a joim research prnject with the Universiry uf Bath which has the following 3 componenrs: 1) cONA c lones for a range uf genes known tn be invulved in wound-respons~s in other plant syste ms wil l be iso lated fro m cassava. based un DNA homology amung functionall y co nse rveu ge nes. These. genes will then be assayed for their expression in cnntrasting cassa,•a genutypes anu co ntrnlled envirn nments affecting PPD. Simultaneously. the cD •A clunes wi!! be includeu in the genetic map nf cassava and monitnred as cand idate genes aff~cting PPD. th mugl1 quantitative trait ana lysis un der representative tie iJ conditions.Genotypes with more than 10 day she lf-life selecreuThe ratio of total sugars to starch co ncentration was positively coiTelated with the le ve! of post-harvest deterioraría n.No correlation was found with dry matter content as previously reported.Wheatley Rationale: eassava represe nts an excellent so urce of starch in the trop ics. dueto its producrion efficie ncy. and the intrinsic characteristics nf the starch produced. e iAT has given emp hasis to the se lel:t ion for higher starch content within cassava gene pools during the last 15 years. As a result of that. a series of e lite ge notypes with hig h co ncentration of starch are currenrly avai lab le at Nacional Prog ram leve !. Not so muc h e mphas is has bee n given to study and deve lop alternative so urces for starch quality. which l:é:tn open new ave nues to bonst the demand fo r cassava. and creare new markets fo r fanners growing the l:ro p in margin al regions. During tlle last 3 years we ha ve ce ntered our eftó rts in 3 areas: a) increasing starch cn ntent: b) characterizi ng starch qual it y within the existing range nf gene tic dive rsity fo r cassava: ande) creare new variants thro ugh genetic transformatio n. We will be reponi ng on the analysis nf starch resistance to di ffere nt treatments in a range nf ge notypes. and the variability in starch granu le structure fnund in the cassava germplasm cu llectio n.:vtethtH.ls: o) Resisrance ro ocitf 111edia and.fi•ee::.ing. Srarch ti•o m 9 genotypes was useu in this :-.tuúy. Thnse genotyp~s were se lected out nf a larger gro up. based nn the range of amylns~ cn nt~nt rWheatley ~tal.( 1992). T he ge notypes were: :VICOL lóX-+: C:'v! J31ló--+: :VIB RA ó2: \\1ME X 5lJ: eG lJ 15-1: :VIVEN 77: MPER 19ó: \\!!YEN 25 : and eG 1 ó5-7. Starch was extrac teJ ma nually. Gcls we re pr~pareu as starch suspensio ns cnntaining 5lfé starch we re prepareu in uisti lleJ water. with th~ aduition of souium be nznate at 0. 11/' c. The suspe nsions were prepared in a Brabe nue r viscnamylog rap h statting at 2 5C anu enúing at ( O C. with an incremenc in tempe rature tlf 1.5 C/minu te.In order tn c\\•aluate res istance to acidity: unce gels reac hed ambient temperatu re. rhey were aciúifíeu at pH 2.-+ with the aúd itio n nf chlor hydric ac id 0.5 0/. and 35 mi of the ge l were stored in 50 mi centrifuge tubes. capped. anu at-+ e during 2. 4. 5 and ó weeks prinr to eva luation. Res istanl:e to freezi ng was ~v alu ated in 50 mi ce ntri fuge tubes. 40 mi of ge !s were sto reú. cappeu. at -20 e uuring l. 2.-+ and 6 weeks. prior to eva lu atinn.Syneresis was measured in lO mi ce ntrifuge tubes. where 5 g of gel from each treatment were weighted. Syneresis was measured by the weight of liquid separated fro m the ge l after centrifuging at 1000 g during 15 minutes at 20 C. and it was expressed as percentage of the total weight. Prior to evaluare viscosity. gel samp les fro m the acidity tria! were left at ambient temperature. while those from the freezing study were left fo r l hour in a hot bath (30°C). Yiscosity was measured in a Brookfield visco meter. with a speed of 10 rpm at 25''C. The Brabender vi.scoamylograph was useli to evaluate reo1ogica1 pro perties of the ge ls. Amylose was evaluated with the cholo rirnetric method 6647 of l. S. O ( 1987).h) Swrch ¡.:ranule shape. A total of 4420 geno types from the global cassava germplasm collection were screened under microscope. after staining with a 0.:2 ~ solution of lugo l (IC I). Those genotypes presenting abnormal granule structure o r associatio ns amo ng granules. were separated for further studies. In acid medium starches sho uld preserve its physical appearance. structure and viscosity during processing and storage. like in the case of thickening agems for sauces. Results from this study showed that cassava starches presented an increase in viscosity after the second week of storage. with the tendenc y to stabilize after that. with the exception of starch from MMEX 59 and MCOL 1684 (Figu re 1.3.3.1). Gels did not show changes in their structure. and no liquid was released (syneresis) during the different petiods of storage.30 t--------.\"\"' •:-:: . .,-::-_ _ __ _ _ _ ----:7'_ .. :::.:. Cassava starch ge l viscosity during storage in acid medium.In Figure 1.3.3.2. changes in viscosity during sro rage of gels at -20 C can be observed. During the ti rst week of storage. al samples s howed a reductio n in viscosity. w ith MYEN 77 and C G 165-7 presenring the greatest reduction ( 11 .7 cp and 10 cp respecti ve ly) . and s howing syne res is at wceks 4 and ó. After 4 weeks in storage syneresis for MYEN 77 and CG 1 65-7 was 4 .4 % and 13.7 c'c-. while after 6 weeks. it was 4.3% and 22.8lk. respective /y. Results demonstrated a re latinnship between amylose co nte nt and the retrogradatio n process (syneresis). Starch prmluced from MPER 19ó and MYEN 25 prese nted the greatest stability during sto rage time . This abnormality can result from different causes: among them mutations in the ratio of amylose/amylopectin. differential regrowth dueto defoliation or lodging. etc. The genotypes will be multiplied this year for fmther studies o f starch functional and chemical properties.e) Srarch phosphorilation. Root and tubers usually ha ve higher Ievels of starch phosphorilation than cereals. The range found in cassava (0.61 to 3.60 nmol Glc6P/mg starch) is very interesting to stan an improve ment process through se lection and recombination (Figure 1 .3.3.3). However. it is considerably lower than the range a!Jeady reponed fo r potato starch (5.0 to 30.0 nmol GlcóP/ mg starch). lt is interesting to notice that the 2 genotypes with the highest Pi le vel (SM X53-7 and CM 743X-I) are ti•om the highlands. Starch synthesized in the high land ecosyste m has particular quality traits dueto the lower average temperature under which it is accumu lated. We are going to use these 2 genotypes as parent lines ro generare reco mbinant progenies and seek for transgressive segregation to r Pi-starch concentration. KVL has deve loped a screening methodology fo r breeders to incorporare Pi-swrch as a selection criteria. A chieve mems:Gcno types with enhanced resistance to acid media and fre ezing conditions have been se lected. C a.ssava germp lasm base has been screened fo r starch granule abnormalities. Genotypes w ith abno rmal shapes and conglomerated structure w ill be studied in detail. Parental material was selected ro develop reco mbinant proge nies fo r the se lection of high Pi-star ch concentratio n in cassava.OUTPUT T lle se /ection o f pare nrs w build po puhnions fo r future breed ing work represents the core nf nur imp rovement ctf orts. since it will determine the genetic progress we w ill ac hieve in the fu ture. T he re are two types of populations deve loped: open pnllinated and contro l crosses. We usua ll y used open po llin ation (po lycrosses) ro deve lop po pu latio ns for target ecosystems. \\Ve ha ve cn nsistentl y deve loped po lycrosses for the sub-humid tropics. acid so il savannas. semi-arid tropics. mid-a lti tude and highland trop ics. and sub-tro pics. I n the case o f co ntrolled crnsses. we used the m to deve lop progenies for specific traits. special studies nr the co mbinatinn nf elite experimental material with loca l landraces that need to be improved.Ge notypes that have bee n selected nve r 2 cn nsec uti ,•e years in ad vanced yiel d trials are nnly ~elected to panicipate as parents fo r the fo llow ing gene ration. A mong those genotypes. we o...elect thuse w ith outstanding per fo rmance fnr the mnst impnrtant agronnmic traits .. -\\fter the analysis of variance is comlucted wirh data across 2 years. tlmse genotypes e.xceeding at leJst une standard deviation from the nveru fl mean are considered as parents t(l r rhe ne.xt gener~Hion. Su meti mes we also include landraces or already re leased culti vars that can co ntribute specia l feaw res tu the proge nies ge nerated.The infonnat inn pn n •ided by Patho log ists. Entomu logists and Qu ality Specialists in re lat ion tu spu rce~ nf re~ista nce or specia l rrai ts is used to se lect genntypes for control crusses. These unmul cro. '>Sc.\\ are dcvelopcd upnn srec ific re4uests frn m ational Programs that want thei r main landrace 1lr released variety crnssed to ge nntypes wi th spec ific traits: or re4 uests frnm C IA.T s scientists that wanr tu pyramid ge nes. m deve lnp segregating pmge nies fnr gene taggi ng.Results: Tub le 2.1.1.1. prese nts the most impo n ant parents se lecte d for the deve lnpment of gene pon ls rargeted ro specific ecosystems. and their performance in relatinn to chec k lnca l nr released \\'arieties. That d i tle r~nce mu ltiplied by the heritabil it y of the traits represe nts the genetic prngre. o...s for next generation. W_ e ca n5~e that we ha,•e been very successfu l in selecting --MCOI:. 146$?: ' : : ::-. ::., , . : : =: : Zli5.J:::::: . .:=:u:::Hi¡.:hla nd tropics .-=::t íñt$ii®t:::::::::::::::::,;,,:,.::,::: 1$~4:t;:::,_::. ,:;~::=::::=::=::' r®.~!~tn::-::,_ :: • : :=:,:::=m::::s~$::1:':'=:::::::: . . , In re latinn to the selection llf parenrs for spec ific traits. ouring the last 3 years we ha,•e incmporatetl ge nnrypes that excel fm the ir: res istance to gree n mires. bacteria! blight. white t1y ano ront rots. ll igll co ncenrration of carotenes antl root ory matter. low co ncenrration o f cyani tle. sllon statu re . excellent cooki ng qua lity. ano broao aoaptation. A total of 3..¡.3 ge notypes se lecteu for their performance in specífic traits ha ve taken pan of our control crosses during the last 3 years. This group represent a broad and se lected genetic base to re-stan our selection process for spec ific traits. incorporare genes fo r specific traits into popular varieties in different countries.and diversify the genetic base for resistance and other traits.Achievements: An average 68% genetic advantage of the best selected parents over check varieties.A gro up of 257 genotypes selected to participare in the generation of reco mbinant progenies that will serve as the basis for future se lection cycles at CIAT and National Program leve !. Snurces of resistance to pest and diseases as well as specific traits selected for the development of contro l crosses.Acrivir v \"•l.\"• Esrablishmenr q{ cmssin;; blocks ami pmducrion t4' recombinanr seed.fimn ¡Jre\\•iouslY eswhlished hlocks.Pnpulations deve lnped fnr specific ecosystems represe nt the basis fnr n ur conperation with :--.Tational Programs and IIT A. T he development of genetic stocks is gaining imponance through the years. Genetic stocks are produced based on the recombination of a set of genotypes that excel for a panicular trait. and we would like to upgrade that trait beyond its natural range of variatinn (i.e. look for transgress ive segregation in broader adaptation ). Stocks developed for inheritance studies nr to suppon mo lecu lar mapping nf specific trairs are cu nstructed by the recombinatio n of co ntrasting genotypes ( i.e. cyanide inheritance). Often times our aim is ro pyramid genes responsible for different sources nf resistance ( i.e. bacteria! blighn. As we shift our emphasis frnm applied breeding to more basic research supponing breeding ( i.e. mnkcular marker assisted selec tion) genetic stocks wi ll beco me e ven more imponant. Parental population develo pment in the tüture will concentrare mnre in targering specific crnsses betwee n genotypes selected by NARS ami comp lementary so urces of genetic infnrmatinn from o ur genetic enhancement prog ram nr our global germplasm collection.F11r pol ycrosses we use the uesign developeu by Wright 1 ~ó5 fnr polycrosses in túrage species. Fnr rhis rype nf design there is a need tll ha\\•e a number of clones equal toa prime number minu s une (i.e. 12. 16. 1 X. ere.). The design allows fur each genotype to ha\\•e the same probab ility u f be ing su rruunded by any other genotype uf the se lected group. Knnwledge nn tlovv~ring capac it y is impmtam in ort.ler tu se lecta group uf mate rials wit h synchronized nmv~ring. When rhere are um-;it.lerable ditlerences we ha ve ro implement delayeJ planting and/ur pruning 11f the mnst earlier tlmve ring genotype-'>. At harvest the seed from different plants nf the same genotype are combineJ together anJ named as a half-sib famil y (S i\\tl ).Fm Cll!Hrol crosses. we plant 1 O tll 20 plants depenJing nn the tlowering capac ity uf the ge nutype in question. Each tlower has the potential to produce J seeds. but in average we nbtain no mme than 1 seeJ per cross. Jue ro the sensitivit y nf the stig ma rn the manipu lation during po llin atio n. SeeJs from the same crnss are mi xeJ togerher anJ name as a full -si b famil y (C :VI)A total of 458.027 recombinant seeds were produced during the period 1999-98 (Table 2.1.2.1). Parental populations aimed at specific ecosystems of co ntinents represented 77% of the total seed produced. Genetic stocks are being built for traits of high priority in our project. In the case of Africa and ACMV resistance. we are developing \" backcross•• populations between Fl crosses (African x Latin American). anda different African parent. The strategy is to ha ve progenies with 75% African background. Stocks for root quality traits represem a large proponion of our efforts. In the case of cooking quality. phosphorilated starch and carotene cnntent. we ha ve crossed genotypes with the highest performance. with the purpose of selecting transgressive segregants within the progenies. For cyanide. white tlies. post harvest deterioration ano bacteria! blight we have crossed genotypes representing the extremes in performance in order to map ano tag the genetic facto rs responsible for the inheritance of those traits. In the case nf dwarf genotypes we are developing a population with combining genetic facto rs deterrnining sho rt plant type. in order to stan breeding for other agro nomic traits with that population.Ac hieveme nts: Co nsiderable amount of recombinant seeds produced. Large proponion of our work shifted to specific traits. through the deve!opmenr of genetic swcks. and pre-breeding pnpulations.More targeted crosses with landraces bui!d duri ng this period. upon request from National Programs.Acti\\•itv..,. 1.3. Cenerarion (in Thai/and) ami disrriburion of\" admn ced hreeding marerials fo r Asian Narional Programs.Breeding for Asia has mainl y ce ntered aro und the issue uf increased producti\\•ity nf dry matter per hectare. Yield and ront dry matter concentration ha ve been the primary traits for se!ectio n. with almost none emphasis give n to pests and diseases. nr cooking quality. The work developed in Asia for 15 years. has revealed the possibility to select fo r broader adaptatinn nf genotypes. We ha ve the case of Rayo ng 60 and Kasetsart 50 with good perfo rmance in a range of Asían co untries. [n nrder to exp loit those two facts plus the need to explore those genotypes in the pipe-line of the Thai breeding program. a considerable imporrance has been given to the production of recnmbinant seed in Thailand. and the transfer of recombinant fam ilies to otl1er co untries in Asia.The sa me approaches as the unes implemented at C lr\\T-HQ (pnlycrosses and contrnlkd crosse.-.) llave been implemented in Thailand. but a greater propnninn nf segregating progenies from cn ntrolled crosses is usua ll y produced.Cluse to 1 ()().()()() were produced duQ.og the last 3 years nf activities. Thirty pe rcent nf that seed was transferred ro -+ Natinna l Programs in the region and ro CIA T/ HQ iTa hle 2. 1.3. 1 ). The retirement uf lHir cassava breeder statinned in Thailand. may resr rict in the futu re. tl1is type nf cn llabo ratinn. from the Thai program. Althnugh it will take longer fnr nther :--.lational Programs ro recei\\•e material.-; fmm Thai land. in the futu re we fmesee tllat the tlux of impn)\\•eu germplasm between CIAT-HQ and the Thai breeding program wi ll continue. and ir \\vil! be through us that uther 1 atiunal Programs will receive progenies invol\\•i ng the latest selections in Thailand.L\"nrestricted suppun and co llabmation from the Thai breeding program. Use uf the most elite ge nntypes in crosses Distribution of seg regating progenies of lligh value for Asian Nationa l Programs. Our strategy fo r cassava germp lasm develop ment is centered on the de ve lopment of improved gene pools for specific edapho-climatic zones with imponance for cassava production. as detined in Table 2.2.1.1. The mosr relevant ecosystems are the semi-arid and sub-humid tropics. fnr which we tlevote the majo rity of our efforts. The main se lection activity is co nducted in sites se lected to rep rese nt the cond itions of the target ecosystem. For every genotype that is tested in those sites we keep a copy at CIAT. CIAT-HQ is conside re<..! to be free of bacteria! blight and some important viruses. and we want to maintain that conditiun. In case we do nnt keep a copy of ~ac h genotype. we would ha veto pass each nf them through quarantine. which usua lly takes mu re than ayear.For each nf the zones we cn nduct a recu rrent selection program. with a progressi\\•e set of stages as described in Figure 2.2.1.1.. As the st:.1ges prngress. we give more emphasis tn traits of lower he ritabil ity. because we ha ve mnre planting material fnr each genntype. and the eva luat ion can be cnnducted in bigger plots witll rep licatinns. Cenain selection criteria are uf gene ral impurtance across ecosystem (i.e. yield potentia l. dry matter content l. while others are specific for each ecosystem ( i.e. pest and diseases).Prng~nies ge nerated fro m the crossi ng blncks (F I) are plante<..! in screen ho uses and transp lanted tn the field afte r 2 months at CIAT. Ató months atter planting. 2 stakes are harvested from eac h plant and given a consecurive number ;,¡ccording ro the plant. One of rhe sta.kes is planteo at CIA T. the ut her nne is p lanted at the m a in se lection si te ( F 1 C 1 ). Selection is conducred at han•est nn individ ual plan ts ar tht:! main se lection site. Planti ng material taken from the se lecred genntypes. at CIA T. is used m estab lis h a 1 w n repl icated ó-plant plot both at CIAT and at the main selectiun site (C ional evaluation stage). E\\'aluation is done using the central .3 plants.Selections are transferred to the fo llowing stage (preliminary yield trial) and planted in non replicated 20-plant plots. Evaluation is done in the central 6 plants. and selections are passed to the advanced yie ld trials at 1 or 2 sites. with 3 replications of 25-p lant plots. Genotypes selected over 2 co nsecutive years at the advanced yield tria! leve! are co nsider as \"elite genotypes\" and incorporated in the germplasm co llection and the crossing blocks. Since each year we initiate a new breeding cycle. we have all the stages simultaneously being conducted in each site. In order tn summarize res ults from the last 3 years nf work. we present the 5 best selectin ns from rile clona! eva luation. preliminary and advanced yie ld trials. fnr the cycle 1997- pcr gennrype. ant.l thc number nf genntypes almost neve r exceed :25. We .se nd information abo ut the families or genotypes that have been prepa.red: and req uest to have fee-back data on the pertormance of the germplasm introduced in order to plan better future shipments.A total of 253.148 seeds correspo nding to 2115 fami1ies and 580 genotypes invi tro were Jistributed during the period 1995-98 from CIAT-HQ to our partners in Latín America. Asia. IIT A and advanced research o r~an izatio ns (Table 2.3.1.1.). The salient feature 'of the shipments has bee n the massive introduction of germp lasm to Africa (TITA). representing 50.-l-% of the total shipme nrs. Previo usly we used to send F1 crosses between Latin American germplasm and elite ge notypes ti•om IITA se lected mainl y to r their resisrance to Ati•ican Cassava Mosaic Virus (ACMV). The frequency of se lections for resistant progenies was considerably lnw in the Fl's. although it was higher than the freque ncy of se lections within proge nies of pure Latín American origin. rn 1995. after ajoint CIAT-IITA meeting. it was decided that in order to increase the frequency nf resistant genotypes within segregating progenies. BC 1 fam ilies. instead nf Fl 's should be introduced. Our interest in increasing the frequency of resistant genotypes is ro allow a more proper selection for other imponant traits that the Latín American germplasm can cn ntribute to Africa (i.e. low cyanide. high dry matter content. good conking qual ity. etc. ). Susceptibility to AC IV has covered up all the potential genetic contribution nf Latín American germplasm in the past. Ta ble 2.3. 1.1. Germplasm distributed ro different resean.:h programs in the world (September 1995 -Sepc 1997). Cnnsiderable i mp nm~d genetic di\"ersity transferred tn Nationa l Prng rams and nther institutions work ing nn cassa,•a. l ncre:.~sed etfic iency of Latín American ge rmpl asm introduction to Africa. thru ugh the de\\'elop ment and shipmem nf back cross seed.Propagation of mapping progenies, inter-specific hybrids and gene tic stocks.Rationale: Given the experience our group has in the managernent of seed ling nurseries and multiplicatio n of genotypes. we provide service to other projects in related to the development and use of the molecular map. gene tagging and introgression of wild gerrnplasrn. By doing so. we promote the integration of advanced tools into our breeding efforts.After obtaining the recombinant seeds. we grow the F 1 nursery for l year. in arder to obtain well de ve loped plants that can produce lO shon cuttings. Depend ing on the project we proceed to multiply each genotype in the progeny using all the cuttings. or we handle half of the cuttings to the scientist interested in evaluating the progeny. and maintain the other half for multiplication. After the tírst mu ltip licatio n we usually give all the resulting material to the interested scientist.The molecular map was originally developed on a pro gen y of 90 genotypes fro m the family CM 7857 . resulting from a cross between MNGA :2 (TMS 300572) and C:'vl 2177-2 (ICA-Cebucán). This cross was designed within our project. taking into consideration the genetic distance berween the parents. and the fact that both parents complernented each nther for traits nf irnportance ( i.e. resistance to ACMV. bacteria! bught. and majar pests. cooking t.¡uality. etc. ). The progeny was later on extended to 150 ind ividuals in order to get a greater nrder nf saturation in the map. The whole family is being multíp lied and evaluated at CIAT. Santander de Quilichao and Villavicencio. Some of the traits for which we are evaluating the progeny include: reaction to bacteria! blight. super-elongation. thrips. root ro ts. post harvest deterioration. root dry matter content. cooking quality. root yield. cyanide. We hope that for some of those traits we can detect specific regions of the genome in between molecular markers with a large effect upon the trait (QTL\"s). which can then be used within our breedi ng sc heme to follo w tlmse genes. Some of the genetic stocks we are rnultiplying at the moment include populations fnr: wh ite tl y: root rot and bacteria! blight resistance. cnoki ng qua lity. post-harvest de terioration. cyanide cnntent and carotene content. All tllose genetic stocks were developed with the aim to tag specific regions of the genome directly in volved in the genetic determination of those traits.With respect to wild Manihot -cassava crosses. we are develop ing fam ilies with parental material selected tHit llf a recent genetic dive rsity stud y using AFLP\"s and micro-satellites. Genotypes from the species M. carrhu;.:t'nl!nsis. M. hruchy/oba. and M. aescu/~f\"olia are being crossed to se lectetl cassa\\•a genotypes. The purpose nf these crosses is tn identify regions of the genome from wild spec ies that can contribute to enhance root yieltl potential ano quality. We are aiming at exp lllring the cn ncept that wiltl species can comribute positive QTL\"s for traits that can not be assessed directly in the wiltl species per se.Genntypes with in target families multiplietJ for basic research projects.Integratio n with projects in the area of biotechnology.OUTPUT 3: National Programs in tropical and sub-tropical La tin America and Asia supported in adaptive selection and deployment of improved cassava varieties.Sub-output 3.1.Activitv 3. l. l.Work together with Nationa l Progra ms in La tin America for the selection, multiplication and dissemina tion of elite cassava germplasmRatin nale: Cassava production is one of the few alternatives to sustain the living of small farmers in sem i-arid regions. That is a result of the capacit y of the crop to survive and produce re latively well under severe water stress conditions. when other crops fail. Ouring pro longed periods of drought in NE Brazil. cassava has been the only source of carbo hydrates and proteins bnth for human and animal consumptio n. Sources of cassava germplasm with improved tolerance to drought. representa ver y va lu ab le resource for homolngous regions in the world where hu man popu lation is expanding into marginal areas to r ag riculture . particular/y in semiarid Sub-sa haran ureas.Within the :--JE-region o f Brazil. a very broad genetic base for adaptation to sem i-arid co nd itions was detected among cassava germplasm accessions. T he explo itation of such wide genetic diversity for the deve lopment of improved genotypes for production under homologous co nditinns represe nts an attractive approach to increase productivity and amel iorate quality nf cassava in the region. because of its low adoption cost and low impact to the environment.Cassava germp lasm evaluation conducted during rhe period 1991-97 in four sites within the se mi-arid ecosystem in NE Brazil. has resulted in the se lection of genotypes w ith improved water use efficiency. That improvement is a co nsequence o f a greater capacity to extraer water stored in the soil. and the possibility of producing large r amounts nf dr y matter per unit of extracted water. Resistance ro mites. alsn constituted a major selection criteria. given the imponance M such biological co nstraint to cassava productio n. As a result of those act ivities. a genetic base for tüture improvement of the cro p in se mi-arid reg ions was estab li shed. At the same time. those genotypes combini ng all desirable agrnnomic traits ha ve the potential to be used by farmers in the target region in the near futu re.Staning in 1997. a second phase fnr the project was financed by lFAD . The main objecri,•es nf the pmject are: to maximize tlle prnductinn pntential o f selected genotypes tl1rnugh the nptimizatin n n f cu ltural practices: w intensi fy the adnptio n and ditlusinn nf imprn ved genntypes wirh rhe act ive participatio n nf farmers: and to generare new genotypes thrnugh the pmcess o f recnmbinati on amn ng cn rnp lementary parents and se lectinn in segregaring progenies.The initial 5year phase nf rhis prnject was sta1ted in 1990 as apure breeding pmject. and ir has evnh•ed thro ugh the years to becnme a more integrated. on-farm. pwticipatory project t(Jr the Ue\\•elopment of the cmp in semi-arid reg inns.Methods: The original work started with the evaluation of 1008 accessions from the cassava germplasm collection held at CNPMF (Bahía-Brazil). Genotypes selected for their broad and specitic adaptation took pan of the on-farm evaluation. An average test will include 9 introduced genotypes anda local check variety. Also, selections from the original evaluated germplasm co nstituted the basis for recombination and generation of segregating progenies that served as the basis to build a breeding scheme for the semi-arid ecosystem.Re.sult.s: In 3 years ( 1994-97). participatory farmer evaluation became the core of the project. with experi mental station breeding activities feeding into it. Alter analyzing informatio n ti•om 2-3 cycles nf panicipatory evaluation there is a list of general fa.rmer selection criteria (Table 3.1.1.1.). which may ha ve sorne resemblance to what we breeders usual! y look in experimental station uials (i.e. productivity. although expressed in different tetms). but refers more to specific concerns from the farmers. Complementary criteria. indude sorne that are location specific. like white roots for Araripina farmers: or good fo liage productio n for fatmers in Quixadá. who use it to teed animals during the dry .season. Now that we ha ve a pretty consistent li.st. we will concentrare in those criteria and .simplify the field book. in order ro make the process of pat1icipatory evaluation a more etfic ient one. One of the teatures that our methodo logy l1as distinguisheú it.self from others in the w•ea of panicipatory research. is the capacity w analyze the informatíon anú proviúe teeúback ro the breeders in terms that are simi lar to the unes they use. T he cumulatíve probability of having a 5<) patticular genotype in a given frumer preference arder (Figure 3.1.1.1.), is a practica! way of visualizing how the mateiials are pertorming in a given ru•ea. Thi.s year we explored a new avenue fo r farmer panicipatory evaluation. We invited a group of expen farmer.s to the experimental .site in Quixadá. for them to se lect. from un intermed iare .stage tria!. those genotype.s that wi ll be evaluated on their farms the t(lllowing crop cycle. They consistentl y .selected genorypes with good prnduction potential at 1 ó-mo harve.st (Table 3 Sorne of them were selected for having good dry matter content according to their testing methodology (chewing: nail: cracking the roots. etc.). Our objective is to pettect this methodology in order to gain efticiency in our breeding efforts for the semi-arid.The project trained 24 extension agents and tield technicians within the PROSERT AO project in Sergipe. That personnel is in charge of evaluating sources of res istance to root rots in conjunction with desi.rability for farmers. We have also trained 5 CNPMFs .scientists in data analysis and interpretation from participatory evaluation trials. Through the teedback information each scientist can take decisions on which technology component should be substituted and which introduced.Our close interaction with other IFAD-funded project (PROSERT ÁO) is based on farmer participatory evaluation of genetic materials generated within our project. and alternative crop management techno log ies generated at CNPMF. Pannerships such as this one. will broaden the scope of our project. and ensure that both the ge netic material and the methodolngy ge nerated with in the project wi ll diffuse and have the expected eco nomic impact.Breeding activities have bee n re-structured. Previous ly al! breedi ng stages were conducted in the 4 experimental sites Otaberaba. Araripina. Petrolina and Quixadá). Since In Ch igh terrility: clay/loam soils. with irrigation): and Betulia (intermediare ferti liry. sandy/lnam soils). The majoriry nfexperimental clones included in this study had high cyan ide amllnr wh ite ronts. characterisrics that made them unsuitable for the fresh market. and more favorab le for industri al production.Results: In spite nf the difterence in snil fertilit y and availability nf water for the differem sites. there was a considerable similarity in terms nf production potentii.ll among sites (6.39 to 7. 7-+ t/ha nf dry maner). Four nf the 1 ()expe rime ntal clo nes were independentl y selected in all sites acruss the 2 ye:.1rs: while 5 clones were se lecred in 3 of the-+ sites (Tahle 3.1.2.1.). Those 9 genotypes are the unes being actively multiplied for furthe r field testing. and eva luation of t!nd-product 4uality (starch nr chips! with rile industry sector. On average a genetic gain n f 35 e;~ \\vas nbsen•ed when t.:n mparing rhe 9 selected genotypes wit h the check varieties inc luded in the c\\•a luation. lmpnl\\'ement in rnot yie ld Wi.lS the main respnnsib le fnr that generic gain. since Jry maner concentratinn re maineJ simi lar to the check varieties. Ac h ie ,.e me nts:Genntypes selected with a 3-+ck highe r dry llli.ltter productio n potential than check vari eties. A netwmk of representative sites for testing genot) pes anoss ~onhern Colombia hüs been established. :VIost llf ou r ctfmts in relation to cassava germplasm development have been concentrated on marginal ecosystems (i. e. semi-arid: low-tert il it y so i ls. etc.). Although cassava is considered i.l resilient crop in terms nf adaptability tn those marginal conditions. it is a species with an acellent capacit y to respond tn added inputs (water. tertilizer. etc.). In fact. under the most favorab le condi tions. cassava is only out-pe rfo rmed by sugar cane in tenns of prnductinn of mega-Gtlories per unit of area (ref.). Although we do not target our breedi ng to the favorable corHJitions in Palmira. all genotypes that are evaluated in sites representing tlle target ecosystems are mai ntained at CIA T. and get to be eva luated. the mid-altitude evaluation we included 4 genotypes that ha ve been declared elite for the region. based on severa! years of experimental evaluation. A total of 17 on-farm evaluation trials ha ve been established. with non replicated 50-plam plots. The on-farm trials are still to be harvested. In order to select genotypes for future on-farm trials. an advanced yield tria! was established in the region. including 29 of the most elite genotypes.Results: a) As a result of that evatuation 9 genotypes were selected (Table 3.1.3.1.) and 7 were substituted for the t997-9R evatuation. The average yield of the 9 se lected genotypes across years was 10.64 t/ha of dry manee. which represe nts a 46.2 % greater production potential than the best se1ections in the Nonhern part of the country (see Section 3.1.2.). We concluded rhat it was possible to select cassava ge notypes with production potential greater than 12 t/ha of dry matter. which represe nt aro und 13.5 t/ha of grains in one year. Unde r this condit ions cassava can ha ve a cn mpetitive margin with higher va1ue cro ps such as sugar cane. partic ularly for the starch 33.93.9 2.X activity is provided by the Ministry of Agriculture of Colombia. Our main partner is FIDAR. a we ll recognize NGO in the highland region. The evaluation included genotypes selected for their resistance to bacteria! blight. and production potential under highland environme nt; along with the local check varieties. We ha ve only harvested l experimental and one on-farm tria! at the moment. since the crop cycle exceeds 12 months. Results from tbis evaluation wil l allow to co llect info rmation on farmer selection criteria in the region. main crop management practices. and to determine the range of adaptatio n of the pre-selected genotypes. We are also multiplying those genotypes in a rder to have enough planting material fo r further diffusion. once final se lection by farmers is made.Ylost of the genotypes evalu ated at the experime ntal station leve!. coincided with those that too k pan on the se lection trials for yie ld potential in high fertility so ils. The soils in the experimental station were aci d (pH 3.7) but with high concentration of organic matter (9.2%). Seven ofthe 13 selected genotypes coincided with se lections made in the more tertile so ils (Table 3 During the co urse uf 1997-98 projects in vo lving cassava activities llave been developed with Paraguay and Cuba. The projects are titled: l.Sustainable deve lnpment and va lorization of cassava in Parag uay: Integration of usernriented crop produ ction. plant pro tectin n and post harvest processing. Eco logic:.llly sustainable plant protectin n and post harvest processing of cassava in Cuba.Bnth projects \\ve re initiateu and re4 uested by nur (CIAT) co llabo rators in eac h of the two cn untries. As can be nnted frnm rhe tit les both projects inc lude a production and post harvest cumponent. In the production co mponent. emphasis is given to germplasm se lection. crop manage ment and integrated pest manage ment. In addit io n bo th projects incl ude train ing ami the use nf fa rmer part icipatory methods. institutional strengthe ning. and ide ntification and imp lementatinn of cassava value -aduing inrerventions.The Paraguay prnject has been se nt co IFAD for funding consideration: the Cuba project has been sent ro IF:-\\D and IDRC. Bot ll funui ng nrga ni zatinns have inúicateu their interest in the projects.óó Activity 3. 1.5. lntegration of privare sector in cassava germplasm development projects.Rationale: A greater pro ponía n of o ur time is being invested in building linkages with the prívate secto r involveti in processing and co mrnercializatio n of cassava. Our aim is to develop cassava germplasm that can better link small poo r farrners to markets in expans io n. When we talk about panicipatory research. we usually refer to the involvement of farmers in the researc h process. It is also important to get other users involved in the process of selection and final evaluatio n (starch processo rs. feeti industry, toa d industry. etc.). A good pro po nio n of the work repo rteo for improving protiuction potential in Northem Colombia and Valle de l Cauca has been co ntiucted in association with starch processors. In the case of the teeti industry. Colo mbia is imp01ting mo re than 90% of the energy co mpo nent that goes into animal teed. Cassava has an exce llent po tential to substitute a great pan of it. The same s ituation can be founti in o ther tropical co untries. Theretore. o ur work has centereti o n improving the competitive capacity of tiry cassava in animal teeti. thro ugh the deve lo pment anti tiiffusio n of imp roveti germplasm anti the o ptimization of protiuction practices.In relation to the tooti market: cassava is being useti as the raw material for a variety of processeti footis (frozen. croquettes. etc.). We ha ve staned a collaboration with a majo r company in that area. fo r the evalu ation anti selection of cassava genotypes with improveti anti stable productivity and quality. We inte nti to draw lesso ns fro m those projects that can be applied to o ther regio ns in the tropics: both fo r the interaction with the privare sector as well as fo r the germplasm tieve lo ped.Methods: One of o ur assistants has been hired untier a commo n funti co ntributed by FENAVI (National Chicken Growers Federation). ACOPOR (Co lombian Po rk Growers Associatio n) and CONGELAG RO (frozen foods). Elite germplasm is being testeo in sites that have bee n prin ritized by the chicken and po rk industry (Valle del Cauca. T o lima. Cund inamarca. Meta. Santander) and the food industry (Quindío). Small multiplicatio n lo ts with genotypes already se lected as e lite materials in previo us years have been estab lished as bas ic seed lo ts. O ur assista nt also provides tec hnical suppon to all the partners invo lved in this project.Results: The project staned in January 19lJX. A total of 95 has ha ve bee n estab lis hed as multiplication. The interaction has invol ved X compan ies re lated to teed prntiuction. anda compa ny involved in food processing. Our goal is to root the idea nf a Latín American cassava fund and research program within the most imponam countries and institutio ns: so they become the main driving fo rce behind the definition nf the research agenda. its financing and execution. There is a need to work with the must imporrant institutions within eac h co untry in arder to define a mechanism of rep resentation to the fund. and for the detinition of research priorities. In the case of germplasm development and diffusinn there will be a need to identify an institution with in-vitrn culture facilities and with certain research infrastructure ro mount an evaluation program. from which the materials can tlow ro lHher institutions in the country. All that work is planed for the year ahead.Achievements: First contact made with institutions from the prívate secto r (mainly relared to processing of cassava). Suppon to the idea of a Latin American fund. expressed by public programs. Ratio na le:The a\\•ailability and LJUality uf planting material will determine the rate of diffusinn and the production potential of improved cassava germplasm. When we talk about LJUality of planting material in cassava. we refer to two aspects: phytosanitary and physiolngical LJUality. Therefme. the productio n of planting materials in areas that are free from the most imponant pests and diseases. as well as the management of the tield lots in terms of the contml uf the biotic constraints and the impro ve ment in so il tertility. play a very impoltant ro le in óX assuring an effective irnpact from our projects in cassava germplasm development. That is the reason why CIAT got invo lved in a project ro build a de-centralized multiplication scheme for cassava. aiming at learning the most and developing methodologies that can be adapted and applied in other tropical countries. The main o bjectives of this project financed by the Ministry of Agriculture of Colombia were: a) to select appropriate cassava varieties ro include in the multiplication scheme: b) to select proper multiplication sites and partner institutions: e) to organize the establishment of multiplication tields. their management. harvesting and distribution system for the planting material: and d) to train farmers and technicians in the management of seed lots.The proposed se heme to be established is presented in Figure 3.1. 7.1. In order to build it from existing material we establ ished certitied seed lots with 3 released varieties: 2 regional varieties and 1 pre-release genotype. with planting material originating in selected tields (free from majar pests and diseases. and with good production potential). Regis ter seed lots were establis hed with 16 elite experimental clones. so rne of which ha ve been selected under onfarm evaluation trials. These two stages we re conducted in 7 sites in Notthern Co lombia. covering an area of 22 has. Basic seed lots were established with planting material from the 3 released and 2 reg ional varieties at CIAT derived from indexed in-vitro plants: to ensure the supply of clean material into future generatio ns. Also at C IAT. we established plots o f elite with planting material derived from plants in exce llent phytosanitary and physiological status. Changes in the mean of the clonal population being evaluated at the advanced stage of regional trials appears to be the best indicator of our selection progress as a who le. This is primarily beca use at this stage a good number of the most advanced materials are evaluated in a multilocation scheme and the clona! enuies represent not only the leve! of selection accomplishment at the moment. but also the possible change that can take place in near future once these entries are released. Genotypes being evaluated at this stage are usually included a<; parentallines. representing the leve! nf breeding materials (hybrid seeds) our CIAT/Thai program offers to other national programs. We can reaso nable assume that the mean of this population represe ms the leve ! of breeding population in eac h year.A.n at.lditional at.l vantage nf ta.king population means rather than the yielt.l data of the best pettormer is ro minimize the error factor. The very high yield of a top pettormer cou ld not be usually repeatet.l. because the major pottion of phenotypic value WJS of non-genetic nature.There is a highl y visible improvement in the actual dry ront yield of the breeding population in rhe past 15 years (Figure 3. 2.1.1.). This significant yield increase (by more than ISO 'k ) must ha ve been attaineó not only by the genetic effects but also by the impmvemem in field management of the yield trials during the same period. Therefore. the comparison of the pnpulatin n mean with the mean of the control (Rayong l) tives a more accurate picture. From th is comparison we can clearly see that there has been a highly convincing genetic improvement in the dry root yie ld leve! of breeding populations (Figure 3 Change in relative mean root dry matter content ( 80 per cent) or as a severe disease with haemorrhagic fever, encephalitis, or retinitis (Soumare et al., 2012). In Kenya, RVF outbreaks have previously occurred in 1931, 1951/53, 1961/63, 1967/68, 1977/79, and In Ijara, an extensive serological survey showed that buffaloes, warthogs and waterbucks had RVFV-neutralizing antibodies, suggesting that these animals were exposed to the virus during the outbreak (Evans et al., 2008). The district also had RVF outbreaks in 1961/1962and 1997/1998(Woods et al., 2002;Murithi et al., 2011).A number of analytical studies have been implemented in the district to identify risk factors for the disease. LaBeaud et al., (2008) A more recent analysis of historical data on RVF epizootics corroborate these findings and shows that high and persistent precipitation over a period of 3 months and low altitude is associated with the incidence of the disease while the presence of soil sub-types solonetz and luvisols in an area leads to persistence of outbreaks for a period of at least 3 months (Bett et al., 2012).Following the 2006/2007 outbreak, ILRI in partnership with the Department of Veterinary Services (DVS) implemented studies in Garissa and Ijara districts to assess the impact of the outbreak and identify ways of improving the prediction, detection, and response to RVF (ILRI, 2007). The study found out that the severity of the epidemic particularly in the northeastern Kenya was exasperated by delays in recognizing risk factors and in taking decisions to prevent and control the disease. The study found out that epidemics of RVF can most effectively be prevented and controlled through the active monitoring of key risk factors leading to timely decision making and the targeting of prevention and control resources.The new transmission studies being done under the HEALTHY FUTURES project build on the work that has been done to further investigate the disease transmission dynamics. They utilize a mechanistic model that simulates the disease transmission dynamics as an analytical framework which specifies the type of data or information required for a holistic assessment of the disease system.RVFV transmission mechanisms are poorly understood partly they involve complex interactions between multiple agents (a wide range of vector and host species) and drivers that operate at local (e.g. socio-economic practices and land use) and regional levels (including climate change). In the Horn of Africa, RVF epidemics occur periodically following periods of prolonged heavy rainfall. It is believed that the virus persists during the interepidemic periods in drought resistant floodwater Aedes eggs. It is also thought that riverine vegetation, moist bushed and wooded grasslands and forests can support endemic transmission of the virus probably because these areas always have high population densities of mosquito vectors and potential reservoir hosts.This study utilizes an individual based RVF model (IBM) as a framework for studying these transmission dynamics. The study area, as described above, is inhabited by transhumant pastoralists whose movements (to and from wet and dry grazing areas) could be important for RVFV maintenance and transmission. Individual based models (IBMs) are suitable for studying such complex non-linear systems where space is crucial and agents' positions are not fixed. They are also useful for simulating agents' behaviours especially if they are expected to change over time as they adapt based on acquired knowledge or in response to new challenges.The model is currently being used to determine types of studies that should be implemented to obtain input parameters. Scenario analyses are also being implemented to generate hypotheses on RVFV transmission mechanisms. The structure of the model is described below.The key components of the model include: (i) the environment or landscape, (ii) agents, and (iii) processes describing interactions in time and space.The model simulates livestock and vector population dynamics and RVF transmission in a spatially-explicit environment that is subdivided into 100 x 100 grids of square cells measuring 500 x 500 m. This framework allows for the incorporation of spatial heterogeneities in the model such as the locations of the grazing sites by season and vector breeding sites. A reliable estimate of the carrying capacity of the area has not been obtained. For the purposes of this analysis, it is assumed that the current cattle and sheep populations of 300,000 and 600,000, respectively, (Department of Veterinary Services, unpublished data) represent equilibrium populations of these livestock species. Sensitivity analyses are, however, being conducted to determine the effects of varying the equilibrium population sizes on epidemic patterns.Vector breeding sites (dambos) are randomly distributed within the grid. The number used at the model initialization stage is given in Table 3.1.Two host species, namely cattle and sheep are used as agents in the model. Their attributes include species (cattle or sheep), age (neonate, weaner, yearling or adult), sex (male or female), infection status (susceptible, exposed, infectious or removed/resistant), time since infection and physical location. Animals are also aggregated to form herds or flocks. The numbers of herds, flocks and individuals generated at the model initialization stage are given in Table 3.1.Dynamic processes that drive the model operations are classified into three, these are: i. Mosquito population dynamics, ii. Host population and movement dynamics, and iii. RVFV transmission dynamics.All of these processes are updated on daily basis. The model considers two RVFV vectors, namely Aedes mcintoshi (indicated throughout this report as Aedes spp.), as the primary vector, and Culex spp., to represent all the possible secondary vectors. Their population dynamics are simulated using a stage-structured transition matrix model described by Yussof et al. (2012) based on the parameters used are presented in Table 3.2. This model illustrated in Figure 3.1. Each vector has four life stages, i.e., eggs, larvae, pupae and adult. Each stage has corresponding probability of surviving and staying in stage i, denoted by i P , and the probability of surviving and growing from stage i to stage i+1, denoted by i G .A list of these development and survival probabilities constitute a transition matrix A and the population of a given stage at time t, X(t), is obtained by multiplying the transition matrix with X(t-1), the population of each life stage at time t-1.i P and i G were computed as described by Yussof et al. ( 2012) based on i S , the survival rate for stage i, and i d the duration in that stage i, as follows:andClimate variables: temperature, precipitation and humidity influence the development rates of most vectors including mosquitoes. At the moment though, only daily rainfall densities obtained from Tropical Rainfall Measuring Mission (TRMM) are used to estimate the development and survival probabilities. Work is underway to include temperature estimates to these functions.For Aedes spp., simulation starts with the hatching of eggs in inundated soils. During dry periods, eggs of Aedes spp that are dormant in dried up soils are assumed to suffer a low baseline mortality rate of μ Ae. When conditions that favour hatching are provided (i.e., flooding that persists for at least 2-3 days), hatching occurs at the rate, H A . Hatching rate is made to depend on the amount of flooding, therefore extensive floods leads to the hatching of a higher proportion of dormant eggs.Larvae develop into pupae after P A days while pupae emerge as adults after E A days. Larvae, pupae and adults have baseline mortality rates of μ Al, μ Ap and μ Aa, respectively. Females seek a blood meal every G A days. Following a successful feeding, these mosquitoes lay eggs on moist soil at the edge of the flooded areas. Aedes spp are assumed to lay S A eggs per batch; all the eggs laid by infected Aedes spp are assumed infected trans-ovarially. RVFV is thought to be transmitted transovarially by floodwater Aedes mosquitoes (EFSA, 2005). Mosquitoes that emerge from the infected eggs develop into infectious vectors. It is assumed the development rates of the immatures, and feeding frequencies and baseline mortality rates for the mature stages are not influenced by RVFV infection. In addition, the model does not as yet allow for the variation in the duration of the gonotrophic cycle, or the number of eggs laid per batch, with an increase in the age of the vector.Culex spp lay eggs on fresh or existing pools of water; these eggs cannot withstand desiccation, therefore they don't remain dormant in the soils like those of the Aedes mcinthoshi. The development processes from eggs to adults are similar to those described for Aedes spp. There is however no transovarial transmission of RVFV in Culex spp or in any other secondary vectors.Persistent rainfall and flooding provide extensive breeding surfaces especially for Culex mosquitoes. Linthicum et al. (1983) indicates that flooding that persist for at least 4-6 weeks allows for the development of massive swarms of secondary mosquitoes which amplify the transmission of RVF when cattle, goats and sheep are present. Similarly, a participatory survey that was carried out in Ijara in following the 2006-2007 RVF outbreak established that the mean interval in days between the start of heavy rains and appearance of mosquito swarms was 23.6 days (Jost et al., 2010). To mimic these dynamics, the number of Culex mosquitoes obtained from the matrix model is amplified based on a by 23-day cumulative rainfall. The cumulative rainfall is also used to control the hatching of infected Aedes spp. eggs that remained dormant in the soils during the dry and low rainfall periods.Model runs generated for this analysis focussed on the period January 1, 2005 to July 23, 2010 so as to capture the recent RVF outbreak that occurred in the district between October 2006 and February 2007. The number of herds and flocks used to initialize the model are given in Table 3.1. These populations represent 1% of the assumed equilibrium population of cattle and goats in the area.A new host is allowed to enter the system through births or purchase while exits occur through mortality, RVFV-associated mortality (case fatality) or through sale; the parameters used to run these simulations are given in Table 3 After parturition, cattle and sheep will undergo a waiting period of 180 and 60 days respectively before it can start breeding again.Hosts move between wet and dry grazing sites depending on season. In the current model, host movements are driven by cumulative daily rainfall. Livestock are confined to the wet season grazing areas when the cumulative (TRMM) rainfall over a period of 21 days is >100 mm/month. Below this threshold, livestock are transferred to a dry season grazing area. Movement ranges within each site are outlined in Table 3.3.The probability that a given host gets exposed to RVFV depends on its level of interaction with infectious vectors present in the area. Given that a host can get infection either from either of the vectors used in the model (Aedes spp and/or Culex spp), the model simulates infection processes for each vector independently and then aggregates them to obtain a composite transmission coefficient, hi  for each host. Parameters that are multiplied to obtain the transmission coefficient for a given vector include:(i) The ratio of the population of the vector species to that of a specified host species, (ii) The vector's biting rate, (iii) The probability that the vector feeds on the host depending on the blood meal index, (iv) The probability that the host gets infected following a bite by an infectious mosquito;(v) The prevalence of RVFV infection in the vector,The composite transmission coefficient ( hi  ) is transformed into host's infection probability ( hi p ) using the formula: A compartmental SIR model is used to simulate RVFV infections in mosquitoes. Susceptible vectors can pick RVFV infection either from infectious cattle or sheep. The transmission coefficient for vector i is estimated by first simulating the interactions between that vector and host species i, followed by aggregating the estimates for all the species that each vector would feed on. Parameters used to estimate this coefficient include: i. vector biting rates ii. blood meal index (indicating the proportion of meals obtained by vector i on host j.iii. the probability that the vector will get infected from an infected blood meal iv. the prevalence of RVFV infection in host i.Following exposure, susceptible mosquitoes will join exposed category for L Ae days (Aedes spp) or L Cu days (Culex sp). They will become infectious at the end of that period. It is assumed that an infectious vector remains at this state for its remaining lifetime. Most of the input parameters for this work have been obtained from the literature. Given the multiple and complex interactions that the model is structured to simulate; it will not be possible to use traditional methods of validation, e.g. fitting the model empirical data. In addition, there is scanty data on temporal-spatial distribution of RVF incidencemost of the available records have been collected during epidemics. Attempts have been made, therefore, to test the model using pattern oriented modelling approaches. This is an attempt to establish whether the model mimics RVF occurrences at different scales and ecologies other than that used to build the model. In this analysis, Arusha region of Tanzania that officially reported the 2007 RVF outbreak in February 12, 2007 was used. Daily TRMM rainfall data for the area were obtained and used to drive the model. Temporal patterns of the RVF outbreak were then analysed against those observed in Ijara.In future, model validation will include testing various parameterizations of the input parameter values to determine how well they simulate observed patterns.Three main studies have been done to generate additional information for RVFV modelling; these include:1. Statistical analyses of historical data on RVF outbreaks in Ijara district to determine the correlation between climate variables (temperature and rainfall) and the outbreaks, 2. Participatory epidemiological surveys to determine types of livestock species kept and their proportions, livestock demographic parameters, and movement patterns, 3. Entomological and epidemiological surveys to determine the risk of RVFV infection in livestockAnnual records on RVF epizootics in Kenya dating back to 1979 were obtained from CDC Kenya. RVF epizootics included outbreaks associated with stormy abortions in livestock especially small ruminants and hemorrhagic syndrome that occurred after prolonged periods of heavy rainfall, and were confirmed using laboratory tests [ELISA] or reverse transcriptase polymerase chain reaction [PCR]). Outbreaks were recorded by year, province, district and area. For the purpose of this analysis, the data were restructured by: (i) classifying the areas affected by divisions defined during the 1999 human population census (n = 505), and (ii) refining the time component of the outbreaks from an annual to monthly time scale. The refinements were made with reference to records kept at the Department of Veterinary Services.Gridded climate data comprising monthly mean precipitation, maximum and minimum temperatures for the period January 1979 to December 2010 were obtained from the European Centre for Medium-Range Weather Forecasts (ECMWF). The data merged with the disease data and kept in a database designed using MS Access database. They were subsequently exported to STATA/SE 11.1 for statistical analysis. A division was used as the unit of analysis. The outcome ( ij y ) represented the infection status (Yes/No) of a division in a given month, therefore it was analyzed as a dichotomous outcome with a binomial distribution, i.e., ) , 1 (Univariate logistic regression models were used to assess the association between the climate variables: precipitation and temperature and RVF outbreaks. Alternative forms of the climate variables were tested; precipitation, for instance, had 7 alternative formulations including:monthly values, -lagged values by 1 and 2 months, -running cumulative values for the recent 2 and 3 months, -running mean values for the recent 2 and 3 months. Maximum and minimum temperatures and NDVI values were used in the analyses as well and competing models compared based on the log likelihood values.The choice of the variables and the lags tested was based on the studies that have been done by Anyamba et al. (2009) which indicates that rainfall, NDVI, sea surface temperatures and outgoing long wave radiation are good predictors for RVF, with NDVI representing ecological variables. Anyamba et al. (2012) also suggested that cumulative rainfall anomaly for 3-4 months immediately preceding an outbreak is critical for RVF outbreaks in East Africa. Temperature does not change much in the area, so lagging was not considered for this variable.Random effects logistic regression models were also fitted to the data to account for clustering of observations in time (due to repeated observations by division). One model had precipitation (3-month aggregate) and minimum temperature as predictors while the other had precipitation as the only predictor. In both models, division was treated as a random effects variable and the correlation structure (for observations within a division) were assumed to be unstructured. The structure of the model used was as follows:Given that the analyses presented in this report were limited to the data from Ijara district, it was not possible to include the other potential predictor variables such as elevation, soil types, land use given that most of their values would be similar.Identification and mapping of the survey sites Participatory surveys were held between August and November 2012 to collect information on livestock demographics and movement patterns. A sub-location, the smallest administrative area with a human population of 4,000 -5,000 was used as the sampling unit. A total of 27 units were selected using stratified random sampling technique from a sampling frame that comprised 40 sub-locations. A division was used as a stratifying variable; the total number of sub-locations per division and the number that were selected are outlined in Table 3.5. One site within a sub-location was purposefully selected for an interview. A site was selected if it had a majority of the families clustered in a small area. Each meeting comprised at least 10 participants and it involved the local pastoralists and community leaders. These meetings were convened with the help of the community animal health workers and the local administrator, which in most cases was the Chief of the area. The meeting sites were geo-referenced after the interview using the Arc 1960 Geographic Coordinate System. Figure 3.3 shows the distribution of these sites within the district.Semi-structured interviews were carried out using the local Somali language with the help of a translator --each session took about 1 hour. The interviews were guided by a checklist of open-ended questions. Probing was also done to investigate other relevant issues that emerged from these discussions. The main items that the checklist covered include:1 Participatory epidemiological (PE) techniques used in the surveys include semi-structured interviews, proportional piling and participatory mapping. These techniques have been described by Cleaveland et al. (2001), Catley and Mariner (2002) and used in several studies including Bedelian et al. (2007) and Bett et al. (2009). Table 3.6 outlines the specific information gathered using each of these methods. Proportional piling is a scoring technique used to determine perceptions on the relative importance, abundance or frequency of a list of items. It uses a set of counters (e.g. beans, pebbles, etc.) that are piled against a given item and then counted to determine relative percentages or proportions. This survey used a total of 100 beans for all the exercises conducted.To determine the relative proportions of livestock species kept, participants were first asked to list the type of livestock species commonly kept in their area. The responses given (e.g., cattle, sheep, goats, chickens) were listed on a flip chart. The participants were then given 100 beans to distribute to the listed items (species) based on the relative abundance of the livestock species assuming that 100 beans represented the population of livestock in the area. Circles were often drawn besides each item to guide the participants on where to place a pile of counters for a species. Livestock species that had the highest population got a bigger pile of beans and vice versa. The piles were counted when all the participants had settled on the distribution provided. They were also asked to give reasons that supported the results observed -e.g. why a particular species was perceived as having the highest/lowest population sizes.The same approach was used for the other proportional piling exercises. For example, on the proportion of abortions/pregnancies carried to term, participants were given 100 beans to represent animals that were pregnant. They were asked to divide the beans into two: the number of animals that was expected to carry their pregnancies to term verses the number that would abort. The exercise was completed for a peacetime period (no major disease outbreaks) and for periods with RVF epidemics. Other exercises involved determining the relative population sizes of age cohorts of cattle, sheep and goats (neonates, weaners, yearlings and adults) identified by the participants, and the relative proportion of animals lost through mortality, sold, or purchased by season.Data obtained from these exercises were entered into a database designed using MS Excel and analysed in SATA 11 using non-parametric statistical tests. Medians and their respective 10 th and 90 th percentile ranges were estimated from the proportional piling scores.Participants were guided to develop maps of their areas indicating human settlements, grazing sites, watering points, roads and service centres e.g. towns. These maps were used to facilitate discussions on a variety of socio-economic activities including livestock grazing patterns. Timelines were used together with the maps to identify locations where livestock were, on a monthly basis, over the period July 2011 to July 2012. Timelines on livestock movements/locations were developed in a reverse order starting with identification of the sites where livestock were in July 2012, and the earliest time (month) when these animals were taken there. This approach was repeated until the full period specified above was covered. Mapping of the livestock movement patterns was done by species (specifically cattle, sheep and goats).Data on livestock movement patterns obtained from the participatory mapping exercises were entered into a database designed using MS Excel. The data variables that could be formulated include: sub-location, GPS coordinates of the interview sites and other locations that had been used for grazing over the year, livestock species, month/year, and an indicator variable which when used together with the month/year specifies whether a given livestock species was just arriving at a given grazing site, had been there for some time or was being moved out to other sites with more pasture/water. Monthly mean NDVI data for all the geo-referenced sites for the study period were obtained from SPOT VEGETATION, filtered and merged with the movement data obtained from the map. Statistical analyses were done to determine mean NDVI values for periods when livestock were being moved out of their recent grazing sites. Up to 1000 bootstrap samples were generated from the sample and used to estimate 95% confidence intervals for the mean NDVI values for each site at the time when animals were being moved out from these areas. These analyses were done in STATA 11 and the results represented thresholds for livestock movement from specific sites. Movement patterns for sheep and goats were combined since these livestock species were often moved to similar locations.Cross sectional surveys were implemented in the district between October and November 2012 at a time when the government had issued a warning on the likelihood of an RVF outbreak. The Meteorological Department had predicted higher than normal amounts of rainfall for the period. Flooding was expected to occur and contingency measures for RVF were being put in place.Eleven sites (10 sites in homesteads area and 1 site in the Boni forest) were selected and used for the survey based on historical information suggesting that these areas had been involved in the 2006/2007 outbreak. In the homesteads, vectors were sampled using CDC miniature light traps placed in the livestock night sheds. This trapping targeted night-time host-seeking mosquitoes. Three traps were set each evening (6 pm) and left overnight and gathered the following morning. Trapping in the Boni forest targeted day-time host-seeking mosquitoes (Plate 3.1). The forest is considered to be a good breeding site for mosquitoes due to high humidity, dense vegetation, presence of hosts for blood meal and presence of water bodies. Samples were barcoded (by trap) and transported alive to a field laboratory where they were sorted, identified to genus level and frozen for storage and transportation.Pooling was done by genus, traps and trapping sites and transported to ILRI Nairobi where they will be subjected to further laboratory analyses to identify blood meal sources, infection status and species diversity.In the same sites, 300 blood samples were collected from cattle and sheep. Herds/flocks sampled included: (i) animals that had just been brought back from the Boni forest (given that the short rainy season was commencing and the pastoralists were bringing their animals back home), and (ii) herds/flocks had not been vaccinated. The sample size (n = 300) used represented minimum number of livestock that would be needed to detect RVFV infection. This number was distributed It was estimated using the formula:where N -the population size (900,000); α -1 -confidence level (0.05); d -the estimated minimum number of diseased animals in the district (population size × the minimum expected prevalence (1%)). This estimation mainly targeted small ruminants (sheep and goats in equal proportions) because they are not usually vaccinated against RVF compared to cattle and the commonly used serological tests do not have the capacity to differentiate infection from vaccination. Cattle, however, can act as good sentinels for RVFV infection because they travel much further than the small ruminants and so they were likely to get greater exposure to mosquito-borne viruses. Limited attention was focussed on cattle in this study because there was no assurance that the project team will get unvaccinated herds.Community animal health workers were used to identify appropriate herds/flocks to sample. For each animal recruited, 20 ml venous blood was drawn from the jugular vein using heparinized vacutainer tubes and transported to the field laboratory where each sample was aliquoted into 5 ml barcoded vials. The samples were then frozen and transported to ILRI Nairobi for further laboratory analyses.District.Ijara district, like those affected by RVF outbreaks in the north-eastern Kenya, has recorded at least 4 outbreaks since 1961 (Table 3.7). Before then, outbreaks were confined to a few districts in the Rift Valley. The data given in the table suggest that all the districts reported outbreaks at the same time.Table 3.8 gives results of univariate analyses that were done to evaluate unconditional association between RVF outbreaks and precipitation, temperature and NDVI. These results demonstrate that RVF outbreaks in Ijara are significantly associated with precipitation and NDVI, which represents ecological changes that promote RVF occurrence, e.g. the development of vector breeding sites. Based on the log likelihood estimates, cumulative rainfall for a recent period of 3 months was strongly associated with RVF outbreaks than the other forms of rainfall variable used in the analysis. In all the sites visited, participants listed cattle, goats, sheep, donkeys and chickens as the common livestock species kept. Cattle, goats and sheep, in that order, are the most abundant and highly valued species compared to the donkeys and chickens (Table 3.10). Participants indicated that they don't keep camels because they are very susceptible to trypanosomosis, the most prevalent vector-borne disease in the area. Wild animals that were identified as being common include buffaloes, warthogs, leopards, cheetahs and a variety of gazelles.Field exercises used to collate data on livestock age structures and their respective risks of mortality required a lot of time to complete. This activity therefore involved a smaller number of villages and focused only on cattle, sheep and goats. Most participants identified at least 4 livestock age categories for each species; these included:cattle: Dalan (0-3 months), Ashirow (4-6 months), Sarar (7-36 months) and Hauwechi (37 months and older); -goats: Dalan (0-3 months), Sarar (4-5 months), Asan (6-12 months) and Riya (13 months and older), and, -sheep: Maqal (0-1 month), Saben (2-3 months), Laah (5-6 months) and Hauwechi (7 months and older). Minimum and maximum ages for each category, relative population sizes, and age categoryspecific risk of mortality were also determined. Younger animals, in general, are perceived to have a higher risk of mortality than older ones. The participants further indicated that mortality levels were higher during the dry than the wet season. Goats are perceived to have a lower risk of mortality compared to cattle and sheep (Table 3.11). Sheep and goats are more likely to be sold (to raise funds that can be used to meet some of the domestic needs e.g. school fees, purchase of grains, settlement of debts and fines etc.) or slaughtered compared to cattle (Table 3.12). Most of the sales occur during the dry than the wet season, with sheep being sold more often than goats. In general, the proportion of animals slaughtered is higher during the wet than the dry season.Findings on a range of reproductive indices such as the duration that young animals take to mature, interval between parturition and subsequent heat, proportion of animals that require repeated services to conceive, twining and proportion of abortions expected during the wet and the dry season are outlined in Table 3.13. Participants, as expected, indicated that females mature earlier compared to males and that dry weather conditions delay both the age at first breeding and the interval between parturition and subsequent heat.In addition, it is perceived that goats have higher proportions of repeat services and higher twinning frequencies compared to the other livestock species. Goats are also perceived to have higher baseline abortion risk relative to cattle and sheep. In all the species, the risk of abortion is higher during the dry than the wet season.Plate 3.2 outlines movement patterns of livestock in Hara sub-location, Ijara district. Similar maps were developed for all the sub-locations visited. General observations made from the mapping exercise are:-Boni forest (located along the Kenya Somalia border), the Tana delta and the banks of River Tana are used as dry season grazing areas. However, Boni forest is heavily infested with tsetse flies, therefore pastoralists move to this site when there are no alternative grazing grounds. Animals are also grazed in conservancies such as the Ishaqbini during the dry season.-Because of the high tsetse challenge in the Boni forest, small ruminants (sheep and goats) are seldom taken there. These animals are often grazed in the peripheries of the forest or in the Tana Delta. In particular, goats are perceived to be more susceptible to trypanosomosis and they are less responsive to medication.-The respondents said that cattle are usually moved out of the wet season grazing sites much earlier than the small ruminants because they are more sensitive to lack of pasture than goats and sheep.-A small herd mainly comprising lactating cows is often left behind in the homesteads when livestock are moved to the dry season grazing sites. These herds provide milk for children, women and the elderly people who remain behind in the homesteads, -Movement between sites could take a short (about 2 days) or a long period (up to 15 days) depending on whether animals can get water and pasture along the migratory routes,To better understand climate thresholds for movement, monthly mean NDVI estimates for the areas where livestock were grazed in during the period considered for these analyses (July 2011 and July 2012) were obtained; these are summarised in Tables 3.14 and 3.15. The overall NDVI mean for the study period was 0.42 (95% CI: 0.38 -0.46).At the time when sheep/goats and cattle were being moved out of a grazing site, mean NDVI values were estimated to be 0.15 (0.08 -0.22) and 0.27 (0.14 -0.40), respectively. These values support observations made by participants that sheep and goats have lower thresholds for movement compared to cattle. A total of 300 blood samples were collected from livestock that were being moved out of the dry season grazing areas towards the end of 2012. Forty nine per cent of these samples were obtained from goats, 35.7% from sheep and 15 % were obtained from cattle that had not been vaccinated. At the same time, vectors sampled (using CDC miniature light traps baited with carbon dioxide) included 2,513 Culex, 33 Anopheles, 9 Mansonia and 7 Aedes mosquitoes.These samples are being analysed in the molecular laboratory at ILRI for RVFV infection. In addition, blood meal sources for mosquitoes are also being investigated using PCR tests.Predicted population dynamics for Aedes app and Culex spp driven by daily TRMM rainfall are given in Figure 3.4.These populations are used to estimate the force of infection, and hence the probability of a host getting infected with RVF virus. Predicted RVF virus infection incidences in cattle and sheep that follow the upsurge in the number of mosquitoes are presented in Figure 3.5.The inset graph in Figure 3.5 demonstrates that RVF epidemics tail off slowly depending on the rate of disappearance of the flood waters. The main graph also indicates that there are periodic occurrences of RVFV related with The model has also been used to conduct a number of scenario analyses. Results of an analysis assessing the effect of varying the area under floods (5 -50%) are presented in Figure 3.7.Predictions given in Figures 3.4 and 3.5 suggest that even though there was heavy precipitation, followed by an upsurge in the number of Aedes and Culex mosquitoes between days 865 to 921, an insignificant outbreak of the disease occurred in livestock at the time. Predictions given in Figure 3.6 suggest that naturally acquired immunity could have played a role in limiting the likelihood of a full-blown epidemic. During this period, peak incidence of the disease in cattle is predicted to have been below 5% since over 60% of the animals were immune. This immunity declined over time such that by day 2000, 40% of the animals were immune. Immunity can therefore play a big role in dampening RVF outbreaks as well as in determining their frequency of occurrence. These analyses are being refined so as to help in determining the duration of herd immunity acquired following RVFV outbreaks. Increasing the number of the mosquito breeding sites increases the populations of vectors, hence the force of RVFV infection, and the probability of an animal encountering at least once mosquito breeding site as it moves around while grazing. Predictions given in Figure 3.7 demonstrate that higher numbers of mosquito breeding sites produces higher incidence of RVFV in cattle that also develops much faster than lower number of breeding sites. The model simulates interactions between the various components of the disease system including vectors, hosts, and the environment and its processes are driven by climatic and socio-economic variables. This approach therefore represents an initial attempt to study how climate drivers interact with local/socio-economic processes such as livestock movements, off-take rates and herd immunity changes to influence the incidence of RVF.The analysis of historical data shows that RVF outbreaks are associated with excessive and persistent rainfall that lasts for a period of at least 3 months. It also reveals that temperature variability is not a significant predictor although these findings will be verified as the statistical model is refined, for example, through the inclusion of other districts and key predictors that could not be used. Nonetheless, similar results have been reported by Anyamba et al. (2012) and they are consistent with observations made by Logan et al. (1991) and Linthicum et al. (1983) that flooding for 10-15 days is necessary for the emergence of RVFV infected Aedes floodwater breeding mosquitoes and that the persistence of floodwaters for a further 4-6 weeks and their colonization by secondary mosquito vectors allows for the amplification of the virus to epidemic proportions. Anyamba et al. (2009) also indicates that RVF outbreaks occur after excessive rainfall and flooding, often associated with El Nino weather phenomenon in the Horn of Africa. El Nino weather patterns follow an anomalous warming of the sea surface temperatures (SSTs) by >1 ⁰C in the eastern-central pacific region and concurrent anomalous warming of SSTs (>0.5 ⁰C) in the western equatorial Indian Ocean leading to increased precipitation (Anyamba et al., 2009). They indicate that in 2006/2007, cases of RVF occurred after 3-4 months of sustained above normal rainfall and associated green-up in vegetation. These observations have been used in setting thresholds for the RVFV transmission model though more work is needed to refine hydrological dynamics that lead to flooding. Analyses on historical data have utilised animal and not human outbreak data although both human and livestock cases were reported in the district during the 1997-98 and 2006-07 outbreaks. Attempts are being made to collate human cases and identify risk factors involved in anima-human transmission so as to estimate the expected impacts of the disease (on both human and livestock health and livestock trade).Animal movements contribute immensely to the transmission and maintenance of infectious diseases. For the purposes of this work, animal movements are classified into three levels depending on the range of distances covered; these are: This report focuses on the second and third levels of movement since these could be relevant for RVFV transmission in Ijara. The local Somali community practice transhumant pastoralism (involving seasonal migration patterns) as the key socio-economic activity to cope or manage the effects of adverse climate. Animals are moved from inhabited areas with diminishing pasture and water to areas where these resources can still be found. Participatory survey established that the number of movements undertaken in a year depends on environmental conditions and the type of animals kept. An analysis of these movements against NDVI estimates as a proxy for climate variability indicates that there is a pattern of increased movement during periods of low NDVI. Small ruminants have a higher NDVI threshold for movement than cattle since they browse on a variety of shrubs that can withstand drought conditions for a slightly longer time than the normal pasture. Similar analyses have been used previously by Worden (2007) to analyse livestock movement dynamics in the greater Amboselli ecosystem in Kenya. Low NDVI estimates, however, might not always imply increased livestock movement because they measure the amount of greenness or green forage that is present in an area rather than pasture availability. In fact drought mitigation strategies focus more on accessing standing dry biomass rather than green forage. Nevertheless, these estimates can be valuable for guiding livestock movement dynamics in the model. It can also be correlated with rainfall density, as it has been done in agronomy, to allow for predictions of future movement patterns assuming that there are minimal changes in land use patterns.Efforts are underway to determine whether seasonal/transhumant migrations influence RVF transmission/persistence. Areas used as dry season grazing sites e.g. the Boni forest and riverine vegetation along River Tana have the potential to sustain an endemic transmission of the virus since they have a rich diversity and density of animals and vectors. Observations made by Shope et al. (1982) indicating that the virus can exists in endemic cycle in forests or in humid and shrubby grasslands are very relevant in this case. Analysis of the biological samples collected from animals that were being brought back from these areas would therefore be invaluable for this assessment. If it is established that these areas have some RVFV activity, then it is likely that exposures that occur while livestock are being grazed there help in sustaining naturally acquired immunity. These hypotheses are consistent with unproven opinions suggesting that major RVF outbreaks occur after prolonged periods of drought when a large proportion of otherwise immune animals are lost, and so they get replaced with naive populations.It has been shown that local livestock movements amplify the rate transmission of an infectious disease especially if movements occur in the course of an outbreak. Anyamba et al. (2010) observe that movement of vireamic animals to other ecological zones in the course of RVF outbreaks amplifies outbreaks especially if these areas have large populations of Culex mosquitoes that play a role in creating secondary RVF transmission foci. Scenario analyses conducted using the RVFV model (not shown) suggest that the range of distances covered per day correlate positively with size (incidence and duration) of an epidemic. This is due to the fact that there is an increased chance of an animal getting into a vector breeding zone the further it moves away from its base.Outputs from the transmission model suggest that herd/flock immunity against RVF can influence the size and intervals of the outbreaks. This appears to be more important in cattle, given their lower turn-over rates, than sheep. It is currently thought that an animal that recovers from natural infection remains immune for the rest of its life. This implies that livestock offtake rates (sales, slaughters and mortalities) are very important in determining the longevity of acquired herd-level immunity by influencing the rates at which immunized animals are removed from the herds/flocks. Preliminary findings show that small ruminants have high turn-over rates compared to cattle. During the dry season for instance, 19% of sheep and 15% of goats are sold to meet some of the household needs. The high offtake rates negatively affect the persistence of herd immunity. The data collected from these surveys will be analysed further and used for the prediction of immunity dynamics over time.There are many other factors that can influence RVFV transmission dynamics which cannot be exhaustively addressed by this report. One of this is the type of hosts that are present in an area (a measure of biodiversity). Participatory surveys identified types of livestock species being kept in the area, their relative population sizes as well as wildlife species that are common in the district. This information is being used to determine types of hosts that should be considered when developing a multi-host model. It is known however that there is a huge variability in the susceptibility of the various animal species to RVFV infection. Domestic animals, for instance, have been listed in a decreasing order of susceptibility as: sheep, goats, cattle, camels and water buffaloes (FAO, 2003). Similarly, antelopes, cape buffaloes, monkeys, cats, dogs and rodents are known to be susceptible while birds, reptiles and amphibians are refractory to RVFV infection. The presence of such a big diversity of hosts in an area can either promote the transmission of the disease (e.g. by providing a larger potential source of blood meal for the vectors or harbouring the virus, etc ) or reduce further pathogen transmission especially is some of them act as dead-end hosts. This is one of the interesting aspect of the topics that would be addressed as the model is expanded and refined.Malaria is a major public health problem in Kenya and it accounts for 30% of outpatient consultations, 15% hospital admissions, and 3-5% inpatient deaths (Njuguna et al., 2012). In arid and semi-arid areas (e.g. Ijara district), malaria transmission is extremely seasonal since the vectors that are involved (mainly Anopheles arabiensis and An. gambiensis) are sensitive to climate variability. These vectors are confronted with highly variable and challenging climatic conditions, particularly during the dry seasons, which cause drastic shrinking or complete disappearance of larval habitats, a decline in the vector population and hence a reduction in the incidence of the disease. Build-up of a new population of vectors in subsequent wet seasons arise either from new populations of immigrants from the neighbouring areas or an expansion of the small local populations that survive the dry period (Mala et al., 2011). Given that Anopheles eggs have low tolerance to desiccation, adults have to survive the dry spell in order for the species to survive by hiding in barrows, abandoned houses, etc.Malaria cases often cluster by geographic/ecological, socio-economic, or demographic factors. In Arid and semi-arid areas, closeness to a river, watering points or irrigated areas has been associated with an increased prevalence of the disease (Oesterholt et al, 2006). Other risk factors that have been reported include living in grass-thatched houses (preferred by mosquitoes), engaging in outdoor occupations such as herding cattle, low altitude, and dense vegetation cover (Mala et al., 2011;Noor et al., 2009). These relationships are, however, not linear; Ijumba and Lindsay (2001) indicate that the use of vector control measures such as bed nets or improved access to medical services masks the expected effects of these risk factors. In fact recent observations indicate that malaria caused by P. falciparum is declining in sub-Saharan Africa due to large-scale bed net programmes and improved case management. Malaria risk mapping work done by Noor et al. (2009) also shows that a large proportion of Kenya (94%) has low intensity transmission which can be difficult or costly to quantify empirically.The intensity of malaria transmission is often measured using: (i) the entomological inoculation rate (EIR), which represents the average number of infective bites per person per unit time, and (ii) Ro, the average number of secondary infections in a non-immune population resulting from a single new infection. However, both of these indices are difficult to measure directly. EIR, for instance, is estimated as the product between the proportion of mosquitoes carrying sporozoites in their salivary glands (sporozoite-rate) and the mosquitohuman biting rate. In semi-arid areas (like Ijara district), sporozoite rate is usually very low and seasonal. Mosquito-human biting rate is also influenced by many factors such as the density of the mosquitoes, relative locations of mosquito breeding sites and areas of human aggregation. Alternative measures for P. falciparum risk have been developed and used since the 1950s, e.g. parasite rate (PfRT), which represents a proportion of a random sample of population with malaria parasites in their peripheral blood, spleen rates, etc. PfRT has been used to map malaria risk in Africa. This is a preliminary analysis that uses hospital records obtained from health facilities in Ijara district to determine whether the number of cases reported in the district can be associated with climate variables -precipitation and temperature. The data represents the number of outpatient malaria cases recorded over a 5 year period and the proportion of the cases that are found to be positive for malaria following laboratory investigation. This analysis is however prejudiced by the fact that hospital records do not necessarily represent the background incidence of a disease. In this case, more work will be done to estimate EIRs and repeat the analysis in order to generate more solid evidence on the linkages between climate and malaria transmission.Hospital records on malaria cases in Ijara district for the period 2006 to 2011 were obtained from the District Health Records and Information Office. The data comprise monthly records of inpatient and outpatient cases; mortalities from the inpatient cases; the number of cases tested versus those that turned positive for malaria following laboratory investigation; and annual quantities of insecticide-treated nets and long lasting insecticide treated nets, artemisinin-combination therapy distributed to people and the number of houses covered with indoor residual spraying.Descriptive analyses were done to explore trends in malaria incidence based on the number of outpatient cases and the proportion that turned positive on laboratory investigation. Subsequently, simple statistical analyses using Generalised Linear Model (GLM) were done to assess the correlation between these outcomes (total number of outpatient cases and proportion of the cases that turned positive) and climate variables: mean precipitation, mean minimum and maximum temperature estimates for the district obtained from ECMWF. Both current and lagged (1 and 2 months) rainfall and temperature estimates were used in the analysis. The dependent variables i y were assumed to have a normal distribution with mean i  and variance 2  represented as:, and the general structure of the model:The two climate variables (precipitation and temperature) were used in the analysis because they have been shown to influence the incidence of malaria. Precipitation influences humidity and causes the development of mosquito larva habitats. Changes in temperatureOutputs from the statistical analyses conducted using the GLM model show that both the number of reported malaria cases and the proportion of positive cases obtained from laboratory investigation are not correlated with either precipitation or temperature (Table 4.2). This analysis explores unconditional relationship between the incidence of malaria and climate (rainfall and temperature) in Ijara district based on cases obtained from the health facilities in the district. This is a simple analysis which is done while recognising the fact that other biological and non-climatic factors are equally important in the disease epidemiology. The records used in the analysis are aggregated by facility/month; this might help to reduce noise in the data. The catchment areas for the health facilities are also quite large relative to population densities. The district has a total of 11 heath facilities comprising one district hospital that serves a population of 100,000 people, one sub-district hospital and three health centres, each serving a population of 30,000 people and six dispensaries, each serving a population of 10,000 people (Njuguna et al., 2012). The representativeness of the data can also be questioned considering the fact that a large proportion of the target population seek medical services from private clinics, pharmacies and traditional healers, etc. which are not captured by the public health surveillance. Nevertheless, the quality of surveillance for infectious diseases has been improving in the country following the introduction of Integrated Disease Surveillance and Response (IDSR) program by WHO and CDC.Most studies have demonstrated that climate factors are important drivers for malaria transmission, affecting both the development rates of the malaria parasites and vectors. This topic has generated a lot of interest because of the expected impacts of climate change on human health. A rise in temperature is expected to increase the transmission and prevalence of malaria by increasing the vector feeding rate and by shortening the incubation period of the parasite in the vector. Precipitation, on the other hand, provides a medium for the development of the aquatic stages of the vector and increases humidity, which enhances the longevity of the vector (Alemu et al., 2011). A recent analysis by Akinbobola and Omotosho et al. (2012), for instance, reported that rainfall (with a lag of one month) and maximum temperature are positively correlated with malaria incidence in Nigeria.Contrary to the expectations expressed above, this study did not find any correlation between climate variables and incidence of malaria in Ijara district. This can be attributed to increased uptake of malaria prevention and control measures such as IRS, ACTs and LLINs. Njuguna et al. (2012) reports that a majority (76.5%) of the cases reported in these facilities are diagnosed using clinical examinations and no laboratory confirmations are done. In fact the degree of positivity that is obtained following laboratory diagnosis rarely goes beyond 40%. This trend is thought to cause an over-representation of malaria incidence and hence an over-treatment. In fact it has been demonstrated that spleen and parasite prevalence in communities that live in villages with health facilities are significantly lower than those communities that live in villages without these facilities (Mboera et al., 2008).There is a need for more studies on the relationship between climate variability and malaria transmission dynamics, and how it is influenced by anthropogenic drivers, including the application of large scale intervention measures. It has been reported that the endemicity and geographical extent of the disease is declining globally, and yet there are predictions that suggest an increased burden of the disease as a result of the global climate change (Gething et al., 2010). This paper also observes that non-climatic factors such as disease control, indirect effects of urbanization and economic development have had greater influence on the geographic extent and intensity of malaria worldwide than have climatic factors.More work is being done to refine the RVFV model particularly on developing an appropriate module to simulate flooding dynamics. This needs to be driven by topography, soil types, precipitation and temperature. There is also a need to develop a way of incorporating wild life, for example having a group of hosts that have variable contribution to the RVFV transmission. In addition, the model does not explicitly include people yet it would be necessary to determine the impact of the disease on humans. This has not been one because it is believed that infections that have substantial impacts are acquired through contact with tissues and (or) fluids of infected animals. This will not be possible to model dynamically. However, a parallel survey is being conducted to identify the proportion of people that engage in risk practices such as slaughtering animals, consumption of uncooked meat etc. to be used for the development of a statistical model that estimates the risk of the disease in humans when there are outbreaks in animals.Biological samples that have been collected so far are inadequate. More sampling will be done particularly in the dry season grazing areas to determine whether they support an endemic transmission of RVF.Finally, RVF is a zoonotic disease and there is a need to collect socio-economic data that can be used to assess factors that promote exposure to humans. This work will be done in collaboration with the University of Nairobi.","tokenCount":"10381"} \ No newline at end of file diff --git a/data/part_6/0800332509.json b/data/part_6/0800332509.json new file mode 100644 index 0000000000000000000000000000000000000000..7023a53e4a7e2b032df13d4514d48ed39fef7856 --- /dev/null +++ b/data/part_6/0800332509.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"b0bcf26780f041cf52764dd6b54f828c","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/35caf4c8-235d-4394-8a1c-d02e41255491/retrieve","id":"896384695"},"keywords":[],"sieverID":"482d2649-f6de-48bd-a066-246ae5d2df23","pagecount":"92","content":"Integrated crop management strategies for plantain production and control of black Sigatoka in DRC The spread of black Sigatoka throughout Venezuela, 1997-2000 Frequency of Paracercospora fijiensis and Pseudocercospora musae in Dominico hartón plantain Effects of the natural fungicide F20 on black Sigatoka disease on plantain and banana Seasonal fluctuations of R. similis and P. coffeae in certain cultivars of banana Host plant response of Pisang Jari Buaya and Mysore bananas to R. similis Effect of three arbuscular mycorrhizal fungi on root-knot nematode infection of Musa Endophytic fungal species associated with root necrosis of banana in Cuba Effects of mycorrhization on micropropagated banana Arachis pintoi: a cover crop for bananas? Dynamics of boron in a soil cultivated with plantain in Colombia Evaluation of the agronomic characteristics of plantain hybrids Options for in vitro propagation of the banana hybrid FHIA-20 Multiplication rate and regeneration potential of somatic embryos from a cell suspension of banana (Musa AAA) Introduction, multiplication and distribution of improved bananas and plantains in Nicaragua Using RAPD technique for identifying and classifying some banana cultivars in Vietnam Consumption and expenditure patterns of banana and plantain consumers in Nigeria Thesis MusaNews MusaForum INIBAP News Books etc. Announcements INFOMUSA Vol. 11, N°1 CONTENTS Integrated crop management strategies for plantain production and control of black leaf streak (black Sigatoka) disease in the Democratic Republic of Congo .The mission of the International Network for the Improvement of Banana and Plantain is to sustainably increase the productivity of banana and plantain grown on smallholdings for domestic consumption and for local and export markets. The Programme has four specific objectives: • To organize and coordinate a global research effort on banana and plantain, aimed at the development, evaluation and dissemination of improved cultivars and at the conservation and use of Musa diversity • To promote and strengthen collaboration and partnerships in banana-related research activities at the national, regional and global levels • To strengthen the ability of NARS to conduct research and development activities on bananas and plantains • To coordinate, facilitate and support the production, collection and exchange of P. Mobambo Kitume Ngongo P lantain (Musa spp., AAB group) is an important staple food in many countries of the humid tropics. It is among the most important carbohydrate sources in the diet of people in these regions. Its low labour requirement and relatively high-energy output make plantain a suitable staple for areas where labour shortage is usually the main constraint to production. The crop is mainly grown by small-scale farmers and it is an integral component of most farming systems in West and Central Africa, where about 50% of the world's plantain is produced (Wilson 1987, FAO 1990).In spite of its importance to local people, plantain has long been ignored by agricultural researchers in the region, since it had no major disease problems until the 1970s and was therefore regarded as a disease-free crop in Africa (Wilson 1987). Twenty-five years ago, however, the crop was threatened by black leaf streak (black Sigatoka), an air-borne leaf spot disease caused by the fungus Mycosphaerella fijiensis Morelet. The disease spread rapidly into all plantain-producing regions of Africa. Black Sigatoka is the most destructive leaf disease of plantain, as it is spreading inexorably to all major lowland plantaingrowing regions as the dominant leaf spot (Meredith and Lawrence 1970). Plantain yield loss of 76% due to black Sigatoka has been reported during the second cropping cycle, while the whole complex of disease, pests and soil fertility decline together reduced yield by 93% (Mobambo et al. 1996a). As a perennial starchy crop, plantain requires a considerable time to mature, resulting in longer exposure to diseases, pests and in depletion of soil nutrients.The soil-disease-pest complex can be controlled by the combination of inorganic fertilizers, fungicides and insecticides/nematicides. In Africa however, chemical control strategies are socioeconomically and environmentally unsound in the framework of the resource-poor smallholders growing plantain. Chemicals are very expensive and their applications may be hazardous to health in the village homesteads where the bulk of plantain is grown. Therefore, proper soil management using several crop residues mulches to improve the organic matter and nutrient content of the soil could reduce the soildisease-pest complex effects on plantain with low inputs.The objectives of the research reported here were to compare field performance and yield of plantain under different practices of soil fertility management and disease control.Investigations were carried out at Kinshasa (4°22'S, 15°21'E, western Congo), which is at 390 m above sea level (Anonymous 1985). The soil of the experimental site is a latosol derived from deposited sands, well drained, but poor in nutrients and highly acidic. Annual rainfall averages 1800 mm and average temperature is 24.5°C.Musa AAB cv. 'Yumba', locally widespread, was used in this experiment. Planting materials still constitute a constraint for plantain production in rural areas. As it is impossible to get many and uniform plantain suckers at once, the investigation started by vegetative multiplication of planting materials (technique described by Auboiron 1997) in order to obtain 625 plants for the experiment: 5 treatments x 5 replications x 25 plants per treatment.Plantain corm stumps were split into sets of 50 g each and treated with wood ashes. They were air-dried for 24 hours before being planted in 15 cm-diameter plastic bags almost filled with forest topsoil. New sprouts emerged after 4 weeks from the date of planting and up to 20 new plants were obtained from a corm.Plants were grown in half-shade conditions and watered regularly. They were transplanted in the field 3 months later, when they had 3-4 true leaves Four different treatments to prevent infection by microorganisms, based on cultural practices, were compared: crop residues mulches (wood sawdust or rice husk), cover crop (Vigna unguiculata) and fertilizers (NPK). Non-treated plants were used as control.The experimental design was a randomized complete block with five plottreatments and five replications. The plot size was 15 m x 10 m with 25 plants spaced by 3 x 2 m, resulting in a plant density of 1667 per ha. Data were recorded only on the nine central competitive plants.Every 3 months, crop residues mulches were applied to the soil around the stem in mulched plots using one head-pan (10 kg). In fertilized plots, 300 kg N, 60 kg P 2 O and 550 kg K 2 O per ha per year were split into six applications during the rainy season: urea at a rate of 65 g per plant per application, phosphorus at a rate of 20 g per plant per application, and muriate of potash at a rate of 89 g per plant per application.For each treatment, soil samples were taken at about 50% flowering stage using a soil hand auger up to 20 cm depth, where plantain has the majority of its roots (Swennen 1984, Purseglove 1988). These samples were air-dried in the laboratory, crushed, passed through 0.5 and 2 mm sieves and analyzed.The disease development was evaluated every week using the \"symptom evolution time\", which is the number of days between the appearance of symptoms of stage 1 of the disease development (Fouré 1982) assimilated to stage b of the cigar (Brun 1963) and the appearance of spots with dry centres (stage 6 of the disease, Fouré 1982Fouré , 1987)). The \"youngest leaf spotted\" which is the leaf with 10 or more discrete necrotic lesions with dry centres (Meredith and Lawrence 1970, Fouré 1982, 1987) and the \"life time of the leaf\", which is the number of days between the cigar-stage b of the leaf and leaf death (100% leaf area necrotic), either due to senescence or black Sigatoka (Mobambo et al. 1994) were also recorded.Disease severity was evaluated every two weeks, from two months after planting until flowering. The percentage of leaf area with symptoms was recorded using the modified scale of Stover and Dickson (1970) as described at the International Institute of Tropical Agriculture (Mobambo et al. 1993a).Growth parameters evaluated include height of pseudostem, girth of pseudostem, number of leaves emerged and height of the tallest sucker. They were recorded on each plant from 2 months after planting until flowering as described by Swennen and De Langhe (1985).Yield parameters evaluated were number of hands per bunch, number of fruits per bunch and bunch weight.Data collected were analyzed using the ANOVA procedures of Statistical Analysis System (SAS 1988) for randomized complete block design. The Duncan Multiple Range (DMR) test at the 0.05 significance level was used to compare treatment means for each parameter.Soil analysis results presented in Table 1 showed significant differences in the amounts of nutrients between crop residues mulches (wood sawdust and rice husk) and other management practices, such as cover crop (Vigna unguiculata) and mineral fertilizer (NPK). Meanwhile, statistical differences were found between rice husk and wood sawdust, with rice husk as the best improving soil fertility level. According to the scales of Black (1965) and Brady (1984), in plots mulched with crop residues the soil was in general moderately acidic with very high organic carbon, high total nitrogen, moderate calcium, moderate magnesium and high potassium. In the non-mulched plantain plots however, the soil was extremely acidic with low organic carbon, moderately low total nitrogen, low calcium, very low magnesium and very low potassium.These results indicate that the amounts of soil nutrients are higher in the mulched plots than in the nonmulched plots. Crop residues mulches constitute better sources of nutrients and act therefore as a fertilizer. As pointed out by Lal and Kang (1982), organic matter constitutes a key component of soil fertility, as a reservoir of nutrients, as a main source of cation exchange capacity and as major promoter of aggregate structural stability.Significant differences were found between plantain mulched with crop residues (wood sawdust and rice husk) and the non-mulched plantain (control, cover crop and fertilizer) regarding symptom evolution time, youngest leaf spotted, percentage of leaf area with symptoms and life time of leaf (Table 2). The severity of black Sigatoka on plantain was much lower in the mulched plantain than in the non-mulched plantain. Among crop residues mulches, however, rice husk was statistically the best for slowing disease development.Black Sigatoka symptom development in the mulched plantain was slower than in the non-mulched plantain. In the plantain mulched with crop residues, the disease needed almost one month more to develop the last symptom stage compared to the control. For the fertilized and cover-cropped plantain, symptom evolution time values were respectively 40 and 36 days, i.e. 2-3 weeks less than for the mulched plantain.With respect to the youngest leaf spotted (YLS), results show the same trend as for the symptom evolution time (Table 2). There were significant differences between the plantain mulched with crop residues and the non-mulched plantain. On mulched plantain, YLS was 11 for rice husk and 9 for wood sawdust, whereas on both the fertilizer-treated plantain and on the cover-cropped plantain the YLS was 8. The non-treated plantain (control) had the lowest YLS value, 6.These results indicate that when using the rice husk mulch the plant gains three healthy leaves comparing with the fertilizers or cover crop treatments, and five healthy leaves against the control. Therefore, with a leaf emergence time of about one per week for plantain in general, the soil fertility (Table 1) due to rice husk mulch slowed the symptom evolution by 3 and 5 weeks compared respectively to that of the fertilized and covercropped plots and the control.Pronounced differences were also obtained between mulched and nonmulched plants regarding the percentage of leaf area with symptoms (Table 2). While in cover-cropped and fertilized plots, plantain presented respectively 10.3% and 6.9% of leaf area infected by black Sigatoka, in plots mulched with crop residues it lost only 3.8% to 4.2% of its leaf area. The slower spread of the disease in mulched plots is facilitated by an increased functional leaf area compared to that of non-mulched plots.INFOMUSA -Vol 11, N°1 Regarding leaf life time, significant differences were also found between plants mulched with crop residues and those that were not mulched (Table 2). Slower disease development on mulched plantain prolonged the lifetime of leaves. In plantain treated with rice husk and wood sawdust, black Sigatoka needed almost 9, 7 and 4 weeks longer to destroy the leaves as compared respectively to the control, the cover-cropped and the fertilized plantain. The control plantain was the most affected by the disease. As already reported, all plantain cultivars (Musa spp., AAB group) over the world are susceptible to black Sigatoka (Fouré 1987, Mobambo et al. 1996b).The difference in the host response to black Sigatoka between the plantain mulched with crop residues and nonmulched plantain is mainly attributed to the difference in soil fertility. The higher the soil fertility level, the lower the black Sigatoka severity. On better soils this is expressed in a slower symptom development, older leaves bearing dry spots, less leaf area with black Sigatoka symptoms and longer life time of leaves (Mobambo et al. 1994).Results presented in Table 3 show significant differences for all the parameters studied: plant height (PH), plant girth (PG), number of emerged leaves (NEL), days to flowering (DF), days for fruit filling (DFF), days to harvest (DH) and height of the tallest sucker (HTS).For all treatments (crop residues mulches, fertilizer or cover crop) the plants had a similar height, whereas they were shorter than the control. However, regarding plant girth and number of emerged leaves, the plantain mulched with crop residues performed better than the non-mulched plantain. Bigger plant girth and lower number of leaves were obtained on plants treated with rice husk and wood sawdust than on the non-mulched plants. Plants mulched with rice husk flowered significantly earlier and had a longer fruit-filling period than those under other treatments. They flowered 5 months earlier than the control and about 1 to 2 months earlier than the fertilized and covercropped plantains. The combined effect resulted in a shorter production cycle for the plantain mulched with rice husk, whose bunches were harvested 104 and 28 days earlier than in the control and fertilized plantain respectively. Plantain mulched with rice husk was harvested 16 days earlier than that mulched with wood sawdust. It also showed better suckering, the tallest sucker, i.e. the sucker that will continue as the next production cycle, being significantly taller than for other treatments. This should normally result in a shorter ratoon cycle for the plantain treated with rice husk as compared to other treatments.The yield components evaluated were the number of hands per bunch, number of fruits per bunch and bunch weight (Table 4).There were significant differences between the plantain mulched with crop residues (wood sawdust and rice husk) and the non-mulched plantain (control, cover crop and fertilizer) regarding the number of hands per bunch and number of fruits per bunch (Table 4). The mulched plantain had a higher number of hands and fruits per bunch than the nonmulched.Yield per hectare was calculated from the average bunch weight multiplied by plant density. Yield was significantly different between the plantain mulched with rice husk and other treatments. The yield of the best-performing plantain treated with rice husk was 46%, 37% and 26% higher than those of control, covercropped and fertilized plantain, respectively. The yield of plantain mulched with rice husk was 14% higher than that obtained with wood sawdust.These results indicate that crop residues mulches confer important advantages to plantain cultivation: higher yield, earlier maturity or shorter production cycle and bigger girth allowing reduction of losses from wind-damage, another important constraint to plantain production (Mobambo et al. 1996a).The research reported here compares different management practices of plantain production. The effects of the crop residues mulches (wood sawdust and rice husk) were compared to those of fertilizer application and cover crop for soil fertility, black Sigatoka severity, growth and yield parameters of plantain.For all parameters evaluated, the plantain mulched with crop residues performed better than the non-mulched plantain. Soil fertility is the critical factor responsible for the difference between crop residues mulches, cover crop and fertilizer. Because of the high level of fertility due to the application of crop residues mulches, plantain was less affected by black Sigatoka and consequently better growing than when receiving no mulch. Among crop residues mulches, rice husk was statistically better than wood sawdust. Therefore, proper management of organic matter is essential for the sustainable productivity of plantain, by minimizing the black Sigatoka severity with low inputs. Since plantain is grown mainly by small-scale farmers in Africa, chemical fertilizers are not readily and economically available. Thus, the potential of traditional organic fertilizers such as compost, farmyard manure and crop residues mulches need to be better exploited. A study integrating organic resources and soil fauna may help to understand the mechanisms regulating the biological processes for the improvement of soil fertility in relation with the sustainability of plantain production, disease and pest severity. S ince the first report of black Sigatoka (Mycosphaerella fijiensis Morelet) in Venezuela, great uncertainty has existed as to the future of banana and plantain production there. The complex nature of the pathogen gives it considerable potential for adaptation to new climatic conditions, fungicides and host genotypes (Ploetz 2000). This is clearly demonstrated by the loss in efficacy of certain products used for chemical control such as the benzimidazoles and triazoles (Douglas and Ching 1992, Estévez 1992, Stover 1993, Guzman et al. 2000, Romero 2000).This situation makes clear the extent of the problem posed by this disease and necessitates the introduction of integrated control measures using resistant clones with a high yield potential (Rowe and Rosales 1993). The existence of a close relationship between certain climatic factors (relative humidity, temperature and rainfall) and the pathogen influences the incidence and severity of the disease (Fouré 1994, Gauhl 1994). It is this which has enabled us to map the spread of the disease across the country and also to take steps against its mediumterm incidence in zones where its presence has yet to be recorded, such as wasThe spread of black Sigatoka throughout Venezuela, 1997-2000Distribution of black Sigatoka done in 1997 and 1998 (Martínez 1997, Martínez et al. 1998).The work presented here aims to describe the current situation of black Sigatoka in Venezuela, the course of its spread, its relationships with various climatic factors which determine its aggressiveness and measures taken for its control. For this purpose, an expedition was made into different areas in the southeast of Venezuela, collecting samples showing typical symptoms of the disease for identification, questioning growers and analyzing weather data (relative humidity, rainfall and temperature).Black Sigatoka was detected for the first time in Venezuela in 1991 in the state of Zulia in the western region (Haddad et al. 1992, Escobar andRamirez 1995), and then it spread into various zones and states (Martínez 1997, Martínez et al. 1998). Mention is made in this report of its arrival in the state of Bolivar and, between 1999 and2000, in the states of Delta Amacuro and Amazonas, in the extreme east and south of the country, respectively. On the basis of their rainfall and relative humidity (Martínez 1997, Martínez et al. 1998), these regions were declared to be at high risk of potential infection in the short term (Figure 1).They are characterized by rainfall in excess of 1500 mm/yr, a relative humidity above 79% and a mean temperature between 25 and 28ºC (Figures 2 and 3), which is significant in view of the relationship established between climate and the incidence and development of the disease (Fouré 1994, Gauhl 1994, Mobambo 1995). They are very different from the Maracay region where the mean rainfall is 922 mm/yr with a 6-month dry season. This situation has enabled the establishment of a model for comparing two totally different agro-ecological states with which are correlated critical levels of the severity reached by the disease. This model serves as a reference for introducing control measures on the basis of climatic conditions and to prevent possible spread into zones possessing similar characteristics (Martínez et al. 2000). Fouré (1994) mentions relationships existing between climatic parameters and the spread of the disease which permit a better understanding of the dynamics of the epidemic in production zones and of its potential for initiating future infection. The liberation of ascospores is rapid during rain because of the presence of a film of residual water on the upper surface of the leaves, whose lower surface exhibits more lesions. Dead leaves which remain attached to the plant therefore represent an excellent source of inoculum (Gauhl 1994). As to the temperature, it is estimated that the ascospores of Mycosphaerella fijiensis germinate between 10 and 38ºC, with an optimum at 27ºC, and noting that the relative growth rate of the germination tubes (hyphae) falls rapidly at temperatures below 20ºC (Pérez and Mauri, cited by Pérez 1996). Concerning the effect of wind, it has been shown that the concentration of conidia in plantations is higher in the lowest air layers than on the leaves whereas the concentration of ascospores in the air is the same: this confirms the importance of the ascospores in the life cycle of the disease (Stover 1984, Gauhl 1994).It remains to emphasize the presence of topographic accidents in well-defined geographical areas which is apparently correlated with the variation in climatic factors mentioned above. These topographic accidents therefore also affect the development and severity of the disease. The first report of the disease AMAZONAS 1991 -1994 1994 -1996 1996 -1998 1998 occurred in the Lake Maracaibo area, where the high relative humidity may be due to the proximity of the lake and to the topography of the landscape of the region. These conditions are similar to those around Lake Valencia (the point of entry of the disease into the state of Aragua) and in the sectors close to the Caroni river, Hato Gil (Bolivar state). In the same way, the presence of the Andes cordillera and the interior mountain chain, which constitute a natural barrier to the passage of the fungal spores to adjacent regions, ought to have prevented the spread of the disease into these zones. However this has happened, and its appearance in these regions can only be due to the transport of infected material.Farm surveys and visits to different areas of the country have shown us that the biggest losses have occurred in fields where there was no control of weeds, nematodes or insects. Hanging, dried-up leaves were not removed and no fertilizer was used. There were also problems of irrigation and drainage and an unsatisfactory spacing of plants in the field. The growers are not in the habit of removing side shoots, nor of using chemicals to control diseases. They lack technical support and resources to buy agrochemicals and equipment. Finally, there are no pro-ducers' organizations. With a low yield which is all consumed by the family, the alternatives for the small producer are to sell his plantation, change the crop or simply to abandon the farm completely (Martínez et al. 2000).Medium-sized producers tend to adjust the area of their plantation if production costs increase, allowing them to obtain yields which are dependent on the amount of investment. Large producers succeed in living with the disease, as can be seen to the south of Lake Maracaibo, where there are associations of producers and firms which improve the quality of the product in the plantations where it is destined for the international market. That which is rejected for export is sold on the national or local market where there is no quality control (Martínez et al. 2000).The presence of black Sigatoka in the country has resulted in radical changes in the way bananas are grown. The traditional approach, which is to manage the plantations as perennial crops, tends to have been gradually replaced by semiperennial, and in some cases annual management of the crop, with high planting densities possibly in association with other short-term crops, giving an increase both in yield and in the diversity of the products obtained. This has been introduced thanks to research work carried out by INIA, and also to the growing importance of the organization among the producers.In the course of all the field experiments the accent has been placed on efficient application of basic cultural practices, such as removal of hanging dead leaves and the use of fertilizer, practices which are not being applied at present even though it has been demonstrated that they help to reduce the amount of inoculum of the pathogen in the plantation and that they render the plant less vulnerable to fungal attack (Gauhl 1994).Quite clearly one should try to reduce the use of chemicals and aim for the best possible way of living with the pathogen. An alternative solution which may be adopted is the use of resistant clones which can either be grown in commercial plantations in rows between the clones traditionally grown in the country (so as to reduce the quantity of inoculum available), or else as an entirely separate plantation, as is seen in the Ocumare region of Costa, in Aragua state. There they have chosen to grow plantain FHIA-21, whose fruit has a softer texture than that of 'Hartón gigante' and can be used for making \"tostones\", or chips, of excellent quality, much appreciated by the consumers, which has facilitated its introduction to the market. Likewise it should be noted that there are other possibilities for production such as the use of the hybrids FHIA-01, FHIA-02 and FHIA-03 which yield well and a have a very good response to the disease.1. The speed of spread of the pathogen through the country has increased rapidly: its passage from the western zone to the central zone took five years while the spread from the central zone to the eastern zone and the south needed only one. It seems clear that this development has been encouraged by man. Causing an increase of 40-45% in the costs of production, the disease has particularly affected small producers and cast doubt over the survival of their holdings. The advance of the disease through the national territory continued into the states of Bolivar, Delta Amacuro and Amazonas between 1997 and 2000. 2. The case of the state of Amazonas, on the frontier with Brazil, is particular. The banana and the plantain, grown by the indigenous communities, are major elements of their diet. The ecosystem of the region is fragile and there is com- Barinas, Maracay, El Vigia, San Felipe and Bolivar (Source: FAV.MARNR, DANAC). Barinas, Maracay, El Vigia and San Felipe (Source: FAV.MARNR, DANAC).plex genetic and biological diversity. For this reason it is undesirable to use chemical control products and preferable to recommend introducing resistant clones which do not need the application of fungicides, even if the cultivars are not fully acceptable to the native consumers. 3. Today it is evident that the presence of black Sigatoka in the country has brought about radical changes in the agronomic management of plantations.The effective use of basic cultural practices, within the framework of integrated control, makes it possible to live with the pathogen, as has been shown by numerous research studies carried out by INIA.C. Lorena Cardona-Sanchez and J. Castaño-Zapata P lantain (Musa sp.) is a subsistence crop and in many areas is the staple food for the population especially in rural areas, with an estimated national per capita consumption of 68.5 kg/annum. Production is mostly with minimal cultivation and as a result yellow Sigatoka and black Sigatoka have increased in severity and dissemination (Merchán 1998), reducing production by 50% (Burt et al. 1997).Nationally 384 957 ha are cultivated, 75% with plantain Hartón and Dominico hartón, the latter cultivar increasingly cultivated in plantain plantations throughout the country, the high com-mercial acceptance being due to their flavour and other qualities and size; it is highly susceptible to black and yellow Sigatokas in the marginal coffee-growing zone (Merchán 1992).Today, these diseases are found competing at altitudes greater than 1000 m above seal level. According to some reports, black Sigatoka, caused by Mycosphaerella fijiensis Morelet, is found attacking plantain Dominico hartón in the district of Victoria (Caldas) 100 m above sea level, and is more aggressive than yellow Sigatoka, caused by Mycosphaerella musicola Leach, a disease which was displaced in less than six months (Merchán 1992). A similar behaviour was observed in the district of Pueblo Rico (Risaralda) 1560 m above sea level (Merchán 1992). According to the latest reports, black Sigatoka can affect plantain from sea level up to an altitude of 1940 m (Belacázar et al. 1994).The Sigatokas are difficult to differentiate from their external symptoms in the field, and hence it is not possible to establish clearly which of the two diseases is more frequent when they occur together (Aguirre et al. 1998b). Microscopically, M. fijiensis and M. musicola are distinguished mainly by the morphological differences of the anamorphs, in particular the conidiophores and conidia characteristics, especially by the presence of scars present on conidiophores and conidia of Paracercospora fijiensis but absent from Pseudocercospora musae (Aguirre et al. 1998b).Management of these diseases may be with chemical products, a practice which is not really applicable to the traditional system of crop production. In order to solve this problem more economical alternatives have been sought, such as host resistance to both diseases which results in a reduced sporulation of the causal organisms.The study was carried out with the objective of determining the frequency of P. fijiensis and P. musae spores in plantain Dominico hartón which is susceptible to black and yellow Sigatoka diseases.The investigation was carried out in the Tolima department, 7 km from the District of Fresno, on the road from Manizales (Caldas) to Mariquita (Tolima) in the village of La Ceiba, Campoalegre estate, located at an altitude of 1250 masl and a temperature range of 18-25 o C, a relative humidity of 65-100% and a rainfall of 1800 mm/annum.First, 1600 plants of the clone Dominico hartón, produced in vitro and multiplied in the tissue culture laboratory, Departamento de Fitotecnía de la Facultad de Ciencias Agropecuaria de la Universidad de Caldas, were transplanted then acclimatized at the Montelindo farm of the University.Evaluations were carried out weekly on 53 clones selected at random. Records were taken starting 13 September 1998, the time of flower initiation, until harvest on 13 March 1999. Impressions were taken weekly from leaves attacked by the Sigatokas with the aim of quantifying spore populations of the anamorph state of M. fijiensis (Figure 1) and M. musicola (Figure 2).Leaf impressions were made with syringes of solidified agar in the form of a dispenser, which was prepared from a 5 ml disposable syringe from which the far end was removed forming a cylinder 1.26 cm diameter. The dispenser was filled with crystal violet agar, prepared by mixing 1 g bacteriological agar with15 ml of a 1% solution of crystal violet in distilled water and 100 ml water. The mixture was autoclaved at 121 o C for 15 min., after which 1 mg benomyl and two sensitivity discs of streptomycin (10 µg) were added (Aguirre et al. 1998b).Conidial populations were quantified weekly by making impressions of each material evaluated, the conidia being removed by pressing the agar surface against the lesion at leaf stage 4-5 of the youngest leaf spotted. The cylinder of crystal violet agar and conidia was placed on a slide and transferred to a tray lined with a paper towel moistened with sterile water. The trays were covered with plastic bags and transferred to a hermetic polystyrene container (icopor).Both fungi were identified and the conidia/cm 2 counted by means of a compound microscope (Olympus) with a 40 x objective.The variables analyzed were the numbers of conidia/cm 2 of P. fijiensis and P. musae, temperature (maxima, means and minima), relative humidity and rainfall.Each variable was subjected to analysis of variance, descriptive procedures for maximum, minimum and mean values, regression, Pearson's correlation and Chi-square test using the SAS (Statistical Analysis System) statistical programme (SAS Institute 1980). Conidial numbers were transformed with Lnx+1, which best fits the behaviour of the data, where x is the number of conidia/cm 2 .Analysis of variance of P. fijiensis and P. musae counts suggested highly significant differences for clones and dates of evaluation. The interactions between the two factors were significant for P. fijiensis and highly significant for P. musae, indicating that a high or low inoculum production depends on the planting material and the effects of environmental conditions on the development of each material (Table 1).The processes of infection and inoculum production were favoured by rainy periods, and as rainfall increased so did the numbers of conidia shown by P. fijiensis and P. musae with two periods of maximum conidial production at 334 and 424 days after planting (dap). These coincided with the maximum rainfall recorded during the study, with an accumulated rainfall of 211.8 mm and 296.2 mm respectively (Figure 3A), which was in agreement with the studies of Aguirre et al. (1998a). The authors observed that accumulated rainfall was inversely related to the incubation period and development of black and yellow Sigatokas, and directly related to sporulation. This suggests that as weekly accumulated volume of rainfall increases, the incubation period and development of both Sigatokas declines and results in an increased disease severity and hence greater inoculum production of the causal fungi. From 424 dap the relationship between conidial number and rainfall started to decline, being particularly evident at 473 dap when, although there was an increased rainfall (192.5 mm), conidial production was very low because foliage was severely necrotic, and no healthy tissue remained available for infection.Temperature and relative humidity remained fairly constant with an average of 21.5 o C (Figure 3B) and 81% (figure 3C), conditions that are optimum for conidial production. In agreement with Mouliom Pefoura and Mourichon (1990) andTapia (1993), cited by Porras and Pérez (1997), temperatures higher than 20 o C favour conidial development in P. fijiensis. According to Stover (1965), temperatures higher than 22 o C favour conidial production in P. fijiensis, with a temperature of 26°C being optimum (Stover * : Denotes significant differences, p = 5% ** : Denotes highly significant differences, p = 1% Note: Data transformed as square root number of conidia. 1965). A relative humidity of about 100% favours production and viability of spores, particularly when a water film is present on the leaf surface (Jacome and Schuh 1992). Conidial populations of P. fijiensis were always greater than those of P. musae in a ratio of 2.3:1 (Table 2) hence confirming that black Sigatoka tends to displace yellow Sigatoka because of its greater aggressiveness; this is in agreement with the studies in the same region by Aguirre et al. (1998a) who demonstrated that black Sigatoka was more aggressive, occurring at times of the year when yellow Sigatoka disappeared, tending to be displaced by black Sigatoka. In general there was a marked direct correlation between conidial numbers of P. fijiensis and P. musae and rainfall, which is also in agreement with the studies of Aguirre et al. (1998a) who observed that fluctuations in the numbers of conidia trapped each week is highly correlated with rainfall.At the time of flowering, which coincided with the start of the study, the standard deviation remained high up to 452 dap, when buds had emerged from most plants; the high standard deviation was due mainly to the effects of rainfall on conidial production as mentioned for the results where P. fijiensis had a higher frequency than P. musae (Table 2).From 319 to 347 dap the accumulated rainfall was 242.5 mm, with a high frequency of conidia of both fungi. However, between 361and 404 dap, the accumulated rainfall of 378.6 mm was excessive, reducing the conidial populations of both pathogens. It is noteworthy that at 438 dap the highest correlation (r = 0.8575) occurred between conidial populations and rainfall (Table 2).Between 411 and 452 dap the majority of leaf tissue started to become necrotic. During this period rainfall was 232 mm and conidial populations an average of 14 conidia/cm 2 /clone for P. fijiensis and 4 conidia/cm 2 /clone for P. musae.From 466 dap onwards, when the cultivation cycle was complete, the standard deviations were low because the majority of clones were severely infected and no more leaf tissue was available for infection, conidial populations of both fungi were low, resulting in a low standard deviation. At harvest, there were an average of 4 and 2 P. fijiensis and P. musae conidia/cm 2 /clone respectively.The conidial populations of P. fijiensis were always higher than those of P. musae, but populations of both fungi declined due to a lack of healthy susceptible tissue (Figure 4).Finally, the relationship observed between the two pathogens followed a regression between the numbers of conidia/cm 2 of P. fijiensis and P. musae in all clones evaluated. The correlation coefficient was very low (r = 0.40656) (Figure 5), suggesting that the number of conidia/cm 2 of P. musae did not depend on the behaviour of P. fijiensis and vice versa, and that conidial production in each of the clones depended on the susceptibility of the material and environmental conditions, particularly rainfall. In spite of the higher numbers of P. musae conidia, the numbers not always increasing or decreasing, the conidia of P. musae followed the same pattern, but there was no direct or strong relationship between inoculum production of the two pathogens.In general, the clones evaluated produced higher total numbers of conidia of P. fijiensis, confirming that black Sigatoka tends to displace yellow Sigatoka (Table 3). ■ he presence of black Sigatoka disease (Mycosphaerella fijiensis) in Cuba since 1990 has resulted in increased costs of production in plantations of plantain and banana because of the increased frequency of aerial and ground-based sprays to control the causal organism. Thus, there is an urgent need to find alternatives with nationally produced products in order to reduce the costs of disease control.The indiscriminate use of chemical products has resulted in side effects including, inter alia, the appearance of fungicide resistance in the causal organism, the formation of strains more virulent than indigenous strains and environmental contamination (Rodríguez and Jiménez 1985, Fullerton and Olsen 1991, Mouliom Pefoura 1999).The use of natural products obtained from microorganisms presents considerable advantages in comparison with commercial products, since their production is much less damaging to the ecosystem and their in situ biodegradability results in compounds that are not toxic to the indigenous microflora. The search for new and different products of natural origin that do not contaminate the environment, for the control of pests and diseases, is an important alternative for sustainable agriculture.Product F20 comprises two antibiotics: the streptothricins B and F. These antibiotics are produced mainly by microorganisms of the genus Streptomyces. The structure has an aminosugar (glucosamine) joined to a ß-lysine peptide chain. Streptothricins F to A differ in the numbers of ß-lysine residues in the peptide chain, from 1-ß-lysine in streptothricin F to 6-ß-lysine in streptothricin A.The physicochemical properties of streptothricins, their spectrum of antimicrobial activity and toxicity are well known (Wienstein and Wagmans 1978).There appear to be no publications on the use of streptothricins for the control of plant pathogens; specifically there have been no reports up to the present on the use of any antibiotic produced by microorganisms against diseases of banana and plantain.In this work we demonstrate the possible use of streptothricins for the control of black Sigatoka disease in clones of plantain (Musa AAB) cv. 'CEMSA 3 / 4 ' and of banana (Musa AAA) cv. 'Parecido al Rey'.The study was carried out at the Instituto de Investigaciones en Viandas Tropicales (INIVIT). Product F20, whose main active ingredients are the streptothricins B and F, was obtained from the Centro de Química Farmacéutica (CQF) in collaboration with the Centro Nacional de Biotecnología de la Universidad Nacional Autónoma, Canto Blanco, Madrid, Spain, by fermentation of strains of Streptomyces lavendofoliae var. 383 (which produces streptothricin B) and Streptomyces rochei var. f20 (which produces streptothricin F), isolated from Cuban soils. The fermentation broth was centrifuged, the supernatant submitted to chromatography on ion exchange resin IRC-50, finally giving a solution saturated with sodium acetate after elutriation with acetic acid.The product was applied after the saturated sodium acetate solution containing the antibiotics was dissolved in an aqueous solution of 0.2 g/L commercial detergent as emulsifier and 60 ml/L mineral oil, to give a final concentration of 5-13 g streptothricin/L, corresponding to a dose of 80-200 g streptothricin/ha. Application was with a knapsack sprayer, with the spray lance modified to simulate an aerial spray of 12 L/h. The product was applied in weeks 8, 13, 17 and 22 to banana, and in weeks 5, 11 and 16 to plantain. Infected leaves were removed at 2 week-intervals for all treatments and mineral oil with 0.2 g/L commercial detergent, as emulsifier, applied to the control treatment in order to ensure their maintenance for the whole of the biological cycle.Each clone separately, plantain 'CEMSA 3 / 4 ' and banana 'Parecido al Rey', were arranged in an experimental design of randomized blocks with six plants per plot, and four replications.The effect of F20 on black Sigatoka was compared with the effect without phytosanitary treatment and with plots treated chemically with propiconazole (Tilt 250 EC) at 400 ml/ha. F20 was applied at a similar dose to propiconazole.The effect of the fungicides was determined weekly by recording the development stage (DS) of the disease, the youngest leaf with symptoms or streaking (YLSS), and the youngest leaf spotted (YLS) (Fouré 1982, Pérez 1996, Orjeda 1998).Sprays with F20 and propiconazole (Tilt) with mineral oil, resulted in a reduction in the disease index DS in comparison with untreated plots (UT) in clones 'Parecido al Rey' and 'CEMSA 3 / 4 '.Figure 1 shows the results of phytosanitary applications in both clones. Graphs A and B show the similar behaviour of F20 and Tilt with DS values not significantly different (P>0.05), and a significant difference (P<0.01) of both applications in comparison with the control treatment.The effects of disease control of the products were also evident in the graphs as a reduction in the numbers of oscillations in DS values and by the amplitude of their fluctuations in both clones. For example, when treated in week 5, DS values declined steadily from weeks 5 and 10, from about DS = 3000 to about DS = 50 in clone 'CEMSA 3 / 4 '; the rate of disease development declined, whereas for control plants, DS values varied between 1500 and 2500.Analysis of the variable YLSS (Figure 2) showed no significant differences (P>0.05) between treatments with F20 or Tilt. However, with clone 'Parecido al Rey' and F20, minor disease symptoms, stage 1, were evident on leaf 9 (Pérez 1996).Figure 3 showed that YLS could reach a value equal or greater than 9 beforeControl of black Sigatoka flower initiation, thus confirming that there were no effects on weight or premature fruit maturity in either clone; in Cuba a strong negative correlation between the leaf area affected and the YLS has been observed (Pérez et al. 1993, Pérez 1996).All the data analyzed above suggest the use of F20, produced from natural origins and mixed with mineral oil and commer-cial detergent as an emulsion, for the control of black Sigatoka disease in banana and plantain crops. F20 is superior to synthetic chemicals in terms of environmental effects. Attention is drawn to the difference in behaviour between the two clones, with 'CEMSA 3 / 4 ' having the higher infection. However, in order to avoid the possibility of fungal resistance, it is important that this product should form part of an integrated control programme in combination with other antifungal products (Pérez 1996, Romero 1997).• Product F20 showed no significant differences in comparison with the commercial product Tilt in its effectiveness to control black Sigatoka. • Maximum effectiveness of F20 was obtained with an emulsion of mineral oil and commercial detergent, and the effect was maintained for 3 or 4 weeks from the time of application. T he lesion-producing nematodes such as Radopholus similis and Pratylenchus coffeae are considered to be the economically important nematode pests of banana and are widely distributed in South India (Koshy et al. 1978, Rajendran et al. 1979). The burrowing nematode, Radopholus similis enjoys wide geographical distribution in the tropical and subtropical banana-growing regions of the world. In India, the first occurrence of the nematode was reported on banana from Palghat District of Kerala (Nair et al. 1966), causing up to 41% yield losses. Subsequently this nematode was reported from banana in South India (Koshy et al. 1978), Gujarat (Sethi et al. 1981), Maharashtra (Darekar et al. 1981), Madhya Pradesh (Tiwari et al. 2000), Goa (Koshy and Sosamma 1988), Lakshadweep Islands (Sundararaju 1990), Manipur (Anandi and Dhanchand 1992), Orissa (Mohanty et al. 1992), Tripura (Mukherjee et al. 1994) and Bihar, Uttar Pradesh and Nagaland (Khan 1999).The root-lesion nematode, Pratylenchus coffeae is reported to have spread to different banana-growing regions through the infested corm. In India, the nematode is known to occur on plantain (AAB) in South India, Gujarat, Orissa, Bihar and Assam (Sundararaju 1996). P. thornei, the other important species was found to infest banana plants from Assam only (Choudhury and Phukan 1990).Crop losses caused by nematodes to bananas are very high, with average annual yield losses estimated at about 20% worldwide (Sasser and Freckman 1987). Soil temperature at a depth of 30 cm did not influence population size (Jimenez 1972). The populations were found to fluctuate between samples, trees, months and years. However, there were definite periods for occurrence of maximum and minimum populations within a year. An extensive survey carried out by Sundararaju (1996) from different banana-growing regions in the country indicated the presence of 17 genera of plant parasitic nematodes. Among them, the lesion nematodes, Radopholus similis and Pratylenchus coffeae are the predominant species found to occur in different cultivars of banana in various intensities. The burrowing nematodeinfested field exhibited severe root rotting, resulting in serious economic losses. Yield was reduced up to 25-35% in the burrowing nematode-infested field compared to nematode-free plantations. Crop losses due to the root-lesion nematode, Pratylenchus coffeae in banana cv. Nendran were reported to be 25.4% (Sundararaju et al. 1999). Therefore, studies were initiated to determine the seasonal fluctuations of these nematode populations in different cultivars of banana roots by periodic sampling of nematode-infested banana plants at the National Research Centre for Banana (NRCB) Farm. The main objective of the study was to find out the activity peaks, in terms of highest and lowest populations of these nematode parasites, in the rhizosphere so that while devising the management schedules, the findings of the work could be considered.In order to study the population fluctuation of lesion nematodes, the three banana cultivars Kalyan bale (AB), Alukkal (ABB) and Kalibow (AAB), which are highly susceptible to R. similis, and a variety Nendran (ABB), which is highly susceptible to P. coffeae, were selected at NRCB Farm, Podhavur, Trichy, Tamil Nadu. The nematode-infested field was selected for this study and the tested banana cultivars were grown in the field and alluvial soil condition. Samples of both soil (250 cc) and root (10 g) were collected from the base of the mother plants at monthly interval from the 5 th month up to harvesting stage during 1997-98. Tender, main feeder roots of white to creamy-white colour, with reddish-brown cortical lesions, were collected from the base of the plants. Care was also taken to collect only the abovementioned type of roots known to harbour the maximum number of lesion nematodes. Root samples washed thoroughly and cut into 2-2.5 cm pieces and later sliced into 8 longitudinal pieces were left in 15 cm-Petri dishes containing 150 ml of tap water for 72 hrs at 10-14°C in a refrigerator for nematode extraction (Koshy et al. 1975). Soil samples were processed as per Cobb's sieving method followed by the modified Baermann's funnel method for estimation of nematode populations. Soil temperature at 15 cm depth was recorded from the fields daily at 7 am. The mean temperature, soil moisture and data on cumulative rainfall were correlated with the nematode population density in the sample.It is seen from Figure 1 that a drastic increase of R. similis population was noticed in all three cultivars during the months of November to April; it later decreased to a negligible level from May to October which was in agreement with Shafice and Mendez (1975). It is interesting to observe that the maximum nematode population was recorded in April from all the cultivars: Kalayan bale (86/g root), Alukkal (78/g root) and Kalibow (68/g root), and the minimum in July in cv. Kalyan bale (20/g root). Analysis of the soil samples also revealed the same trend as in the case of root samples with maximum population occuring during the month of November to April with maximum rainfall and soil moisture during the period. In the case of P. coffeae on variety Nendran the maximum population was recorded from October to December and the minimum population from May to August (Figure 2). Regarding the average population for a month, it is seen that a maximum of 92 per gram root was recorded in December, whereas it was only 23 per gram in June. The root-lesion nematode population from soil samples also showed the same trend as in the case of root samples with maximum population during the months October-December and minimum population during the months May-August. Peak rainfall occurred during the Northeast monsoon (September to December) with an average rainfall of 140 mm. The soil temperature at 15 cm depth recorded from fields varied from 18-37.5°C. Analysis of the moisture content revealed that it was maximal in those months where maximum nematode populations were recorded (Figure 3). Rainfall also influences the growth of roots. Thus, with the increase in the availability of root system, there was an increase in the activity of R. similis during November to April and of P. coffeae during October to December.Fluctuations in the populations of Pratylenchus spp. were correlated with rainfall (Cooke and Draycott 1971). The behaviour of P. coffeae in relation to soil temperature and rainfall was similar to that of P. crenatus and P. penetrans in corn (Miller et al. 1972). Lack of moisture coupled with high summer temperature during April to August was found to be unfavourable for the prevalence of P. coffeae in oil palm (Sundararaju and Ratnakaran, in press). The present investigation is in agreement with Kumar (1984) who reported that higher population of P. coffeae was recorded during the month of October to December which is the period of high rainfall and increased root activity in coffee plants.Similar observations were reported in the burrowing nematode R. similis on citrus (DuCharme and Suit 1967), banana (Vilardebo 1976), coconut and arecanut (Koshy and Sosamma 1978).Figure 1 indicates that the R. similis population fluctuates between the months. A steady increase of R. similis population was recorded during the months of November-January and gradual decrease was recorded in February and March, whereas a drastic increase of nematode population was recorded in April in cv. Kalyan bale (Figure 1). A similar trend was noticed in cvs. Alukkal and Kalibow (Figure 1).In the case of P. coffeae a steady increase of nematode population was recorded from September to December and gradually decreased from January to June (Figure 2).This clearly shows that the population build-up of R. similis and P. coffeae would greatly vary depending upon the season and other ecological conditions such as rainfall, soil temperature, soil moisture and availability of susceptible roots which play their own roles in the population build-up.Duong Thi Minh Nguyet, A. Elsen, Nguyen Thi Tuyet and D. De Waele P lant parasitic nematodes are a major constraint of banana production worldwide (Gowen and Quénéhervé 1990). Nematode infection can interfere with nutrient and water uptake and transportation, resulting in slow growth, reduced fruit filling and sensitivity to wind lodging. Among the nematodes attacking banana, Radopholus similis (Cobb) Thorne is considered the most destructive species (Sarah et al. 1996).The possibilities of controlling nematodes in bananas are limited because bananas are usually grown as a permanent crop by small-scale farmers and sources of resistance have proved hard to find. Resistance to R. similis has been reported in 'Pisang Jari Buaya' (Musa AA-Pisang Jari Buaya group) and 'Yangambi Km5' (Musa AAA-Ibota group) (Pinochet 1988, Viaene et al. 1998, Fogain and Gowen 1998, Stoffelen 2000). The clone 'SH-3142' derived from a genotype belonging to the Pisang Jari Buaya group and 'SH-1734' was found to be highly resistant to R. similis (Pinochet andRowe 1979, Pinochet 1988). Moreover, some 'Pisang Jari Buaya' expressed favourable agronomic features similar to those of commercial banana.The Mysore banana (Musa AAB) is a very popular and delicious dessert. Information on resistance and/or tolerance to R. similis of Mysore bananas is scarce. When testing 17 AAB Musa genotypes, Fogain (1996) reported that none of the plants were immune, including 'Pisang Ceylan', the only cultivar belonging to the Mysore group. The objective of our study was to further investigate the host plant response of Musa genotypes from the Pisang Jari Buaya and Mysore groups to a R. similis population from Costa Rica, to find additional sources of resistance to the burrowing nematode.Throughout the study, the terminology of Bos and Parlevliet (1995) concerning resistance and susceptibility of host plants to pathogens and the methodology for nematode resistance screening in Musa as described by Speijer and De Waele (1997) were used.Thirteen diploid (AA) banana genotypes belonging to the Pisang Jari Buaya group (Experiments 1 and 2, see Tables 1 and Table 2) and five triploid (AAB) banana genotypes from the Mysore group (Experiment 3, see Table 3) were included in the study. Two triploid (Musa AAA) bananas, 'Grande Naine' and 'Yangambi Km5', were included as reference genotypes because of their high susceptibility and resistance to R. similis, respectively. The Musa genotypes used in the experiments were provided by the INIBAP Transit Centre (ITC) at the Catholic University of Leuven. After proliferation, regeneration and rooting (Banerjee and De Langhe 1985), each in vitro propagated banana plantlet with 3-4 leaves and 5-6 roots was transplanted in a 1-litre (12 cm-diameter) plastic pot containing about 1000 cm 3 autoclaved substrate of peat and quarts (2:1). To keep a high humidity, the pots were placed under a plastic cover, which was slightly opened after 2 weeks and removed after 4 weeks. The greenhouse conditions were maintained at 25-30°C and 70-80% relative humidity with a 12-hour photoperiod. The pots were irrigated as needed and fertilized with a hydroponics solution (Swennen et al. 1986) every 3 weeks after nematode inoculation. The plants were inoculated with nematodes either 4 weeks after planting for the Pisang Jari Buaya group, or 8 weeks after planting for the Mysore group, since the number of nematodes was too low in the experiment with Mysore genotypes.The R. similis population used in the experiments was obtained from infected banana roots of 'Valery' (Musa AAA) at Talamanca in Costa Rica. The population was reared monoxenically on carrot discs and incubated at 28°C in the dark for several generations (Moody et al. 1973, Pinochet et al. 1995). The carrot discs were blended twice for 10 s (with 5 s interval) and poured through 106 and 25 µm pore sieves. Carrot tissue collected on the 106 µm pore sieve was discarded, while the nematodes were collected from the 25 µm pore sieve.A suspension of 1000 living vermiform nematodes was poured in three holes made in the substrate around the base of each plant. After inoculation, the holes were covered.Eight weeks after inoculation, the plants were harvested to observe the response of the different banana genotypes to R. similis. The following data were recorded:The procedure followed was that described by Speijer and De Waele (1997). Five 10 cm-pieces of functional primary roots were collected and sliced longitudinally. The percentage of root cortex showing necrosis was scored for a half of each root. The maximum root necrosis per root half is 20%, giving a maximum root necrosis of 100% for the five root-halves together.The entire root system, including the 5 roots segments observed for necrosis, was weighed and cut into 2 cm-pieces. Fifteen grams of fresh roots were taken randomly and macerated three times for 10 s with 5 s intervals. The mixture was poured through a series of 250-106-40 µm pore sieves and the sieves were rinsed with tap water. Nematodes remaining on the 40 µm pore sieve were collected in a beaker with distilled water. Nematodes were counted in 6 ml aliquots of each sample using a binocular microscope.Three experiments were conducted, based on a completely randomized design, with either eight replicates for each genotype (Pisang Jari Buaya group, Experiment 1, Table 1; Mysore group, Experiment 3, Table 3) or nine replicates (Pisang Jari Buaya group, Experiment 2, Table 2). Prior to statistical analysis, the percentage of root necrosis was transformed to arcsinNematode resistance (x/100) and the nematode numbers were converted to log 10 (x+1). All data were subjected to analysis of variance (ANOVA) and means of the parameters were compared using the Tukey HSD test at P ≤ 0.05.The results obtained from the Pisang Jari Buaya group are presented in Tables 1 and 2. In Table 1, no significant differences were observed in nematode numbers per root system or per 1 g fresh roots and root necrosis percentage between the Pisang Jari Buaya genotypes and 'Grande Naine'. Among the Pisang Jari Buaya genotypes, root necrosis percentage was significantly higher in 'Morong Princessa' compared with 'Pisang Tunjiuk' and 'Saing Todloh'. In These results show that all Pisang Jari Buaya genotypes tested are as susceptible to R. similis as 'Grande Naine'. They confirm a previous report (Wehunt et al. 1978) that 'Pisang Jari Buaya', 'Gabah Gabah', 'Pisang Sipulu' and 'Pisang Gigi Buaya' are significantly less sensitive to root damage (expressed as root necrosis percentage) compared with 'Grande Naine'. Surprisingly, 'Pisang Jari Buaya', which has previously been confirmed resistant to R. similis (Pinochet 1988, Viaene et al. 1998, Fogain and Gowen 1998, Stoffelen 2000), did not appear to be so in our study. Also, 'Pisang Sipulu', considered a promising banana genotype because less susceptible to R. similis (Wehunt et al. 1978, Binks andGowen 1996), did not show resistance either to R. similis in our study.The results obtained from the Mysore group are presented in Table 3. The nematode numbers per root system and per 1 g fresh roots of 'Gorolo' and 'Lady Finger' (South Johnstone) were significantly lower compared with 'Grande Naine', while those recovered from the other Mysore genotypes were not significantly different compared with the reference genotype. The root necrosis percentage observed in 'Thap Maeo' and 'Gorolo' was significantly lower compared with 'Grande Naine'. In contrast, the root necrosis percentages of 'Pisang Ceylan', 'Lady Finger' (South Johnstone) and 'Lady Finger' (Nelson) did not differ significantly from those in 'Grande Naine'.According to Price (1994) and Price and McLaren (1995), AAB Musa genotypes are susceptible to R. similis when examined in field trials. Unfortunately, genotypes of the Mysore group were not included in their trials. Our study confirms previous reports (Stanton 1994, Fogain et al. 1996) that 'Lady Finger' water and mineral nutrients from the soil. AM fungi also may protect plants against soil-borne plant pathogens, including nematodes. Several studies have addressed the associations between AM fungi and root-knot nematodes, which are considered the most important nematodes in the western hemisphere on temperate agricultural crops. Many mycorrhizal associations are reported to have a suppressive effect over sedentary endoparasitic nematodes. In some crops this effect is significant enough to consider mycorrhizal infection as a more or less effective means of biological control (Pinochet et al. 1996).In bananas, only a few studies were carried out on the effects of AM fungi on nematode development. Radopholus similis populations in the roots as well as in the soil were suppressed in mycorrhizal plants compared to non-mycorrhizal plants (Umesh et al. 1988). Under in vitro conditions, using Ri T-DNA transformed Daucus carota roots, a R. similis population was suppressed with 50% in the presence of AMF (Elsen et al. 2001). Pinochet et al. (1997) reported that mycorrhizal colonization did not effect nematode build-up in the roots, although plants infected with both Meloidogyne javanica and Glomus intraradices were more galled.In this experiment, three Glomus species (G. mosseae, G. macrocarpum and G. caledonium) were tested on the Musa cultivar Williams (ITC0570) for their effect on Meloidogyne javanica, a root-knot nematode population isolated from banana in Morocco. Tissue-culture derived plantlets were acclimatized in 1-litre pots filled with sterilized soil in the greenhouse. During transplant the plantlets of the mycorrhizal treatment were mycorrhized with soil inoculum, consisting of ± 1850 spores and 0.25 g mycorrhized roots from Allium porrum. After one month, the plants were inoculated with a mixture of 5000 M. javanica juveniles and eggs. The experiment was planned as a 4 x 2 randomly factorial design with 8 replicates per treatment: AM fungi (-AM, G. mosseae, G. macrocarpum and G. caledonium) x M. javanica (+ M. javanica, -M javanica). Three months after planting, the 'Williams' plants were harvested and assessed for mycorrhizal colonization and nematode damage/development. A sub-sample of the roots was stained with 0.05% trypan blue in lactic acid (Koske and Gemma 1989), in order to determine the mycorrhizal colonization. The galls on the roots were counted in a 5 g sub-sample after staining with phloxine B (Hadisoeganda and Sasser 1982).The AM fungi had no effect on the plant growth since shoot weight, shoot diameter, plant height and root weight did not differ among the treatments (data not shown). In general, mycorrhization of banana plants resulted in a better plant growth compared to non-mycorrhizal plants (Declerck et al. 1994(Declerck et al. , 1995)). Although, in some cases, it has been observed that the establishment of the symbiosis resulted in a negative or neu-tral effect on plant growth as long as the mycorrhizal colonization was not well developed (Jakobsen 1998). Therefore at the time of harvest, the root colonization by the three Glomus strains tested was relatively low. This may partly explain why in this experiment no effect on plant growth was observed. In addition, it is important to note the differences in colonization among the Glomus species in the plants without nematodes. Higher colonization was observed with G. mosseae as compared to G. caledonium and G. macrocarpum. Such differences were also reported in literature (Declerck et al. 1994(Declerck et al. , 1995)). Glomus mosseae was shown the more infective on 'Williams' and other cultivars, as compared to G. macrocarpum (Declerck et al. 1995).Glomus caledonium and G. macrocarpum significantly reduced galling in the roots, while for G. mosseae this reducing effect was not significant (Table 1). In literature, results are contradictory: according to Pinochet et al. (1997), Glomus intraradices did not reduce nematode build-up of M. javanica and resulted in more galled roots compared to non-mycorrhized roots. In contrast, G. mosseae suppressed root galling and nematode build up of Meloidogyne incognita (Jaizme-Vega et al. 1997).Meloidogyne javanica significantly decreased the intraradical development of G. mosseae. For G. macrocarpum and G. caledonium no such effect was observed: the presence or absence of the root-knot nematode had no effect on internal root colonization. In similar experiments, root-knot nematodes had no effect on the percentage root colonization in mycorrhizal plants (Pinochet et al. 1997, Jaizme-Vega et al. 1997).The results of this experiment suggest a suppressive effect of the three Glomus strains studied over the rook-not nematode M. javanica. Mechanisms involved in nematode suppression are still a matter of speculation. However some major factors are likely to be involved: enhanced nutrient status of the plant, biochemical changes in plant tissue (increase in chitinase, amino acids, peroxidase and phytoalexins), anatomical changes (increased lignification), stress alleviation, microbial changes in the rhizosphere and induced changes to root morphology (increased branching, larger proportion of higher order roots) (Hooker et al. 1994). Further study is needed to confirm the suppressive effect of the AM fungi over the root-knot nematodes and to reveal the mechanisms involved. ■ A. Battle-Viera and L. Pérez-Vicente I n Cuba a total of 108 700 ha are cultivated with Musaceae, 32 800 ha with cultivars of banana subgroup Cavendish (AAA), 13 800 ha with plantain (AAB) and 62 000 ha with varieties of type Burro/Bluggoe (ABB). Of the 32 800 ha of banana plantations, 13 800 ha are cultivated with localized microjet irrigation systems, and they must therefore remain in place for the next five years. However there is interest in replanting these areas with tetraploid hybrids developed by the Fondación Hondureña de Investigación Agrícola (FHIA), and which are resistant to pests and diseases.The nematode species most commonly found in our plantations are Radopholus similis, Pratylenchus coffeae, Helycotylenchus multicinctus, Meloidogyne spp. and Rotylenchulus reniformis, the first three being the most important in Cuba (Pérez et al. 1984). The pathogenicity of nematodes has usually been established as the population density found in the roots. However, evidence on the relationship of population density of nematodes to damage in the crop is contradictory. Over the last years plant lodging and root necrosis in Cuba have been recorded where low populations of nematodes were very low.Interactions at the root level between R. similis and species of fungi belonging to the genera Cylindrocladium and Acremonium, which contribute to or increase damage by the nematode, are well documented (Booth and Stover 1981, Loridat 1989, Sarah 1990). Such associations have been found in the majority of soils infested with nematodes in some of the Antilles islands. In Cuba there have been no studies to investigate and quantify such relationships at the level of the root. However the relationship between nematode populations, root damage and plant development is not strong.The purpose of the present study was to identify the species of fungi associated with root necrosis of different clones of banana and plantain in plantations in Cuba.Samples were taken from banana plantations located in the provinces of Pinar del Río, La Habana, Matanzas, Villa Clara, Ciego de Avila, Camagüey, Cienfuegos, Santiago de Cuba and Guantánamo.Samples were taken from necrotic roots of plants of Gran enano (AAA), Gros Michel (AAA), CEMSA 3 /4 (AAB) and Burro CEMSA/ Bluggoe (ABB), some of them associated with plants that had fallen over (lodged) apparently as a result of nematode attack. From each field 10 plants were selected at random, holes 20 x 20 x 20 cm excavated 10 cm from the pseudostem and five affected roots removed.The roots were washed and necrotic fragments, typical of R. similis attack, disinfected in 1% hypochlorite for two minutes and cultured on water-agar supplemented with 50 µg/ml streptomycin. Blocks of agar and fungal growth were transferred to tubes of PDA, and incubated when the fungal species were ready for identification. Fusarium species were identified according to the key of Booth (1981). Cylindrocarpon species were identified according to the keys of the CMI edited by CAB.The relative frequency of each species present at each site was determined in relation to the total numbers of isolates obtained from the different sites.A total of 59 isolates of endophytic fungi were obtained from the tissue of roots apparently necrotic as a result of R. similis. The species are described in Table 1.Species of Cylindrocarpon musae and Fusarium oxysporum Schlect. were isolated from almost all samples from all the sites. F. oxysporum was the most frequently isolated species (45.6% total isolates), followed by C. musae (19.2% total isolates). F. equiseti (Corda) Sacc. was also isolated but with less frequency. The results were similar to those of Pocasangre (2000) who found that Fusarium species were predominant in soils from Cuba, Costa Rica, Guatemala and Honduras. Booth and Stover (1871) reported the presence of C. musae associated with root necrosis in banana in Costa Rica, however the fungus did not have the parasitic capacity to cause lesions on healthy roots. Other species of Cylindrocarpon are important pathogens of plants. For example C. destructans causes root necrosis and death in pine (Pinus sp.) (Chakravarty and Unestam 1987).Recently there have been bioassays of artificial inoculation of C. musae alone or of C. musae in co-inoculation with R. similis. The studies provided important information on the effects of the pathogenicity of these species on root necrosis in the crop.None of the sites yielded species of Cylindrocladium or Zythia as reported by Loridat (1989), Mourichon (1993) and Risède (1994). These species have been associated with necrosis of banana in Martinique and Guadeloupe, and latterly in Cameroon (Abadie 1998, pers. comm.) and Côte d'Ivoire (Kobenan 1991). Cylindrocladium and Zythia spp., reported in other countries to be associated with root necrosis, were never found.Effets of micorrhizae M.C. Jaizme-Vega, M. Esquivel Delamo, P. Tenoury Domínguez and A.S. Rodríguez Romero T he likelihood of using arbuscular mycorrhiza (AM) in crop production systems is increasingly more realistic and studies have increased considerably in the last few years.Banana (Musa AAA) in its early stages of development is readily colonized by mycorrhiza and is moderately (40-50%) dependent on them (Jaizme-Vega et al. 1998). Mycorrhization in vivo has resulted in large increases in the growth and nutrition of this species (Lin and Chang 1987, Rizzardi 1990, Declerk et al. 1995, Jaizme-Vega and Azcón 1995) including in the presence of standard fertilization regimes in commercial nurseries (Tenoury 1996, Sosa Hernández 1997), with favourable effects on plant behaviour when confronted with various soil-borne pathogens such as Meloidogyne incognita (Jaizme-Vega et al. 1997), Pratylenchus goodeyi (Jaizme-Vega and Pinochet 1997) and Fusarium oxysporum f.sp. cubense (Jaizme-Vega et al. 1998). These results demonstrate the advantages of applying inoculum of fungal AMs during root production and acclimatization of micropropagated banana plants, which gives rise to plants that are well developed and have an increased tolerance to attack by soilborne pathogens. However, at present there is no information on the effects of such symbiotic fungi on the banana plant during the later stages of development and with fertilizer regimes similar to those practised in commercial crops.Therefore, the sequential effects of early mycorrhization on the growth of micropropagated banana plants were studied from the earliest stages of development until nine months after transplanting to the field in microplots.Micropropagated material of the two most widespread commercial cultivars of banana Musa acuminata Colla AAA, cvs. 'Grande naine' and 'Gruesa' (a local selection of 'Dwarf Cavendish') was used.Mycorrhization was done during hardening off. Inoculum comprised a homogeneous mixture of rhizosphere soil, spores and rootlets of the host plant.Each cultivar was inoculated with one of two AM fungi, each with 1500 g inoculum per tray (capacity of tray 24 kg) with the following isolates: At inoculation, plants were 10 cm ± 2 cm and had approximately three developed leaves. Inoculation was in polyethylene (PE) trays (40 x 60 cm, H x L), each tray containing one cultivar/fungus combination with an additional two control trays with non-inoculated plants, one tray per cultivar. Thus there was a total of six trays each with 35 plants.The substrate comprised a steam-sterilized mixture of dark-coloured volcanic soil and amended peat (TKS1®, Instant, Floragard, GmbH) in a proportion of 5:2:1. This phase lasted six weeks in a glasshouse and under a tunnel of black mesh for acclimatization. Irrigation was with distilled water according to the needs of the plants.At the end of root production and before transplanting to individual containers, 10 plants of each treatment/cultivar combination were selected and the effects evaluated of inoculation with mycorrhiza on plant development, the mycorrhizal dependency under the fixed conditions of fertilizer inputs, and the extent of colonization by the AM fungi.Parameters relevant to the growth of the plant in general were evaluated at each stage of the investigation as follows: fresh weight (g) of roots and aerial parts, dry weight (g) of aerial parts, length and diameter (cm) of pseudostem, leaf numbers and area (cm 2 ). Leaf area was calculated with an area meter Li-COR, inc. Lincoln, Nebraska, USA, model Li-3100.The relative mycorrhizal dependency (RMD), defined by Gerdeman (1975) as the degree of mycorrhization needed by plants to produce the maximum growth or yield depending on fertility of the soil, was calculated according to the formula proposed by Plenchette et al. (1983) as the numerical expression of this concept: Infection by the mycorrhiza was confirmed by observation with a light microscope. Root samples were bleached with 10% KOH and then stained with 0.05% trypan blue in lactic acid as described by Phillips and Hayman (1970) and modified by Koske and Gemma (1989). Percentage root colonization was determined on 20 1-cm sections of stained root, mounted on slides and examined with a light microscope as described by Brundett et al. (1985).Once the determinations were complete 20 plants of each treatment were transferred to 2 L PE bags containing a substrate comprising equal volumes (1:1:1) of steam sterilized soil ('picón') and enriched peat (TKS1®). This phase took 14 weeks in glasshouse conditions at temperatures of 27-32 °C, and a relative humidity of 70-80%.Fertilization was according to the fertilizer regime of a commercial banana nursery. Plants were fertilized twice weekly (100 cc/plant) on alternate days. One of the fertilizer applications was with (NO 3 ) 2 Ca (3 g/L) and NO 3 H (0.4 cc/L), and the other application was with SO 4 K 2 (3 g/L) and PO 4 H 3 (0.2 cc/L). The days on which fertilizer was not applied alternated with irrigation with running water according to the needs of the crop. Plants received a weekly foliar application of micronutrients consisting of 3% Wuxal® Super AA 8-8-6 (Argos Shering, Agrevo, S.A., Valencia, Spain).After growth for 3.5 months, plants were transferred to larger containers, and buried in a plot within the boundaries of the ICIA estate situated 300 masl. The site was chosen on the basis of aspect, climatic conditions and as an area marginal for this crop. Prior to this, and as with the first transplanting, 10 plants per cultivar and treatment were evaluated for the effects of the AM fungi, that is the extent of root infection by mycorrhiza and mycorrhizal dependency.For this last phase of the trial, PE pots 35 cm diameter and 50 L volume were selected and filled with non-sterilized medium of the same materials and in the same proportions as described for the previous transplanting (1:1:1), and amended with 1.5 g/L of slow release fertilizer (Osmocote 17:10:10, Scotts, O.M. Tarragona). Once in position in their new pots (10 per cultivar and treatment), the plants were placed amongst other similarly sized pots previously buried up to the upper edge of pot, in the trial plot. Plants were fertilized weekly (1 L/plant), via the localized irrigation system, with the two combinations of fertilizer treatment described previously for banana plants after the first transplanting. Foliar fertilizers were applied fortnightly. The days on which fertilizers were not applied, plants were irrigated according to the needs of the plants.Plants remained in position for nine months. The trial was then terminated and the effects of symbiosis on development of the banana plants evaluated.The following experimental variables were studied: fresh weight of roots and aerial parts, numbers of suckers, numbers of leaves, leaf area, N, P and K content, and dependency for mycorrhiza.On completion of the foliar analyses, the samples were transferred to a heater for 24 hours at 70°C after which nitrogen, phosphorous and potassium contents were determined. For N determination, the sample was mineralized \"via humid process\", P was determined colourimetrically and K by spectrophotometry of atomic absorption.Data were analyzed by means of ANOVA (Systat). Means were compared by Fisher's test of least significant differences (LSD) using the statistical package Systat version 5.0 (SPSS Inc., Chicago, USA).By completion of the rooting stage, both cultivars showed a positive response to the two AM fungi used for inoculation (Tables 1a and 2a). In this phase, the relative mycorrhizal dependency (RMD) of both cultivars to Glomus manihotis and Glomus intraradices were the highest throughout the trial and were 35% and 50% respectively. In this first phase the percentage colonization by the two inoculated AM fungi was similar for the two cultivars. Following transplanting, the positive effect of the AM fungi on plant development was maintained for 3.5 months after mycorrhization. For inoculated plants of both cultivars, the majority of experimental variables were significantly different for both cultivars in comparison with the controls (Tables 1b and 2b). The development of RMD was similar for both cultivars completing this phase of the trial with averages of 40% for both AM fungi on 'Grande naine', and 30% and 20% respectively for Glomus manihotis and Glomus intraradices on Gruesa (Tables 1b and 2b).Root colonization of banana plants by mycorrhizae tended to differ depending on the cultivar. Thus, roots of 'Grande naine' inoculated with G. manihotis had twice the mycorrhiza infection in comparison with the beginning of the study, similar results being maintained on roots colonized by G. intraradices. However with plants of cv. 'Gruesa' no changes in root colonization were observed in comparison to the first transplanting. During the trial, from 14 weeks onwards 15% root infection by contaminant AM fungi was noted in control plants of both cultivars (Tables 1b and 2b) but without signifi-cant effects on plant development. These endophytes are able to disperse in irrigation water or by uncontrolled contamination in the nursery containing the plants.These data confirm those already published on the benefits of early mycorrhization of plants in the first phases of development of this crop (Declerck et al. 1995, Tenoury 1996, Sosa-Hernández 1997, Jaizme-Vega et al. 1997, 1998).The results of the second phase of the trial in which the effects of the AM fungi on mycorrhiza-treated plants in the in vivo phase for three months and transplanted to non-sterile medium, showed that after nine months in microplot conditions and a standard fertilizer regime, banana plants inoculated with G. intraradices usually, particularly with cv. 'Gruesa', showed a beneficial effect of Table 1. Effect of Glomus manihotis and G. intraradices on the development, colonization and mycorrhizal dependency of micropropagated banana cv. 'Grande naine' at a) 6 weeks after inoculation, b) 14 weeks after inoculation and c) 9 months after transplanting to microplots. 1c and 2c); moreover there was an increase in the other experimental variables. However, data on macronutrients (N, P and K) although noticeably higher, did not differ statistically (Tables 1c and 2c). This lack of response in nutrient content of aerial parts can be interpreted as typical for a mycorrhiza-treated plant receiving soluble fertilizer. Plants of cultivar 'Grande naine' showed a smaller response to the AM fungi after the microplot phase, plants inoculated with G. manihotis showing a development and nutritional state equal or slightly less than control plants.At the end of this phase, root colonization by both Glomus species was relatively important in both cultivars (greater than 79%). Attention is drawn to the high level of colonization of roots of control plants. This part of the trial used nonsterilized substrate which, together with other conditions in the trial, explained the data.In conclusion, in general and particularly in the last phase of the trial, we can confirm that, at the later stages of the crop, this biotechnological resource showed promise for the improvement of production. T he legume Arachis pintoi L. (the perennial, wild or pinto peanut) has been used for many years as a cover crop in many tropical countries, notably Central America (Kerridge 1993). Its response to nematodes is still barely documented. Possible resistance of cv. Amarillo to Meloidogyne spp. in Australia has been mentioned (Cook et al. 1990). In Mexico a noticeable reduc-tion in Meloidogyne attacks on tomatoes was observed in an intercropping experiment (Marban Mendoza et al. 1992). In Costa Rica, a field experiment showed that Arachis pintoi is a good host for Radopholus similis (Cobb 1893, Thorne 1949) with a concomitant mean infection rate of about 30 individuals per g of root (Araya 1996). Also in Costa Rica, trials carried out with bananas and plantains have shown a beneficial effect of wild peanut used as a cover crop in reducing the density of Radopholus similis on neighbouring banana plants (Vargas 1998). Lastly, in 1999, Jonathan et al.showed from an artificial inoculation experiment that the legume Arachis pintoi is not a host for certain species of Meloidogyne Goeldi 1892 (M. incognita, M. arenaria, M. javanica) nor for Rotylenchus reniformis Lindford & Oliveira 1940.Before experimenting with, and perhaps recommending the use of Arachis pintoi as a possible cover crop for bananas, we wanted to check its behaviour towards nematodes of banana in Martinique. A controlled inoculation trial using the main species present (Radopholus similis, Pratylenchus coffeae, Hoplolaimus seinhorsti, Meloidogyne incognita) together with Meloidogyne mayaguensis, a species which is very pathogenic in Martinique although not yet observed on bananas, was therefore carried out in a controlled environment chamber at the IRD Nematology laboratory before attempting any field experiments.Seeds of Arachis pintoi cv. Amarillo from Costa Rica were inoculated by coating at the moment of sowing with their symbiotic bacterium Rhizobium sp. These seeds were then grown in 237 cm 3 PVC culture tubes filled with sterile soil (steam sterilization for 1 h at 100ºC). The substrate was a volcanic andosol of pH 6.2, with 7.3% organic matter and a cation exchange capacity of 10.3 meq per 100g soil. The experiment was carried out in a controlled environment chamber with eight replicates, using a 14-h photoperiod and a temperature in the light of 27±1ºC, and in the dark of 22±1ºC, daily watering and weekly application of a Hoagland nutrient solution. Four weeks after sowing and development of the peanut, the five nematode species, meanwhile grown in the laboratory (Radopholus similis, Pratylenchus coffeae, Hoplolaimus seinhorsti, Meloidogyne incognita and Meloidogyne mayaguensis) were individually inoculated at a rate of 400 individuals per plant. The infestation of the root system was checked 45 days later after extraction of the nematodes from the roots by spraying (Seinhorst 1950). The nematode densities were then expressed as numbers of nematodes per root system and per g of dry root (after oven-drying at 60ºC for 24h).The results of this experiment (Table 1) show that at 45 days only three species of nematode are supported: R. similis, H. seinhorsti and P. coffeae. The inoculation of the different species of nematode has had no effect on the growth either of shoots or roots of the peanut, which therefore appears, over this short period of time, to be tolerant of attack by these nematodes.Arachis pintoi was unable to maintain or permit the multiplication of the two species of Meloidogyne, M. incognita and M. mayaguensis. This result confirms and completes for M. mayaguensis earlier results on the inability of this peanut to act as host to the main species of root-knot nematodes, with the exception of M. hapla (Jonathan et al. 1999).Arachis pintoi does however act as host to three other species, and according to the criteria applied to banana weeds (Quénéhervé et al. 2002), one might say that it is a bad host for R. similis but a very good host for H. seinhorsti and P. coffeae. The hosting capacity of Arachis pintoi to R. similis already observed (Araya 1996) is thus confirmed, but also (which is new) its great susceptibility to P. coffeae and H. seinhorsti, two species of nematodes whose pathogenicity to bananas was demonstrated for one (P. coffeae) and seems very likely for the other.These results may be compared with others from the field under conditions of natural infestation. In fact, root produc-tion in Arachis pintoi is extremely slow, with a shoot/root ratio of about 7.5 in our experiment, and it would be interesting to quantify the real \"reservoir\" capacity of this plant for nematodes in the field as was done by Araya in 1996. However one can already regard it as a \"non-host\" for Meloidogyne spp., and especially M. mayaguensis, and reconsider this crop as a \"cleaning fallow crop\" (for rehabilitation) which provides protection against nematodes before a susceptible annual or perennial crop.For many years agronomists have sought plants useful as fallows of short, medium or long term, or as cover crops which can, inter alia, reduce parasitic pressure (for example by nematodes) and also reduce the effects of weeds, improve soil fertility and limit erosion (Terisien and Melin 1989). In the Caribbean zone, two species have been used for their activity against nematodes, with both advantages and disadvantages: the forage grass Digitaria decumbens and the forage legume Mucuna pruriens cv. utilis, of African origin.Each of these plants has its value according to the cropping system being considered. Digitaria decumbens fits into long-term rotations combining livestock and field vegetable growing, as practised on the vertisols in the south of Martinique. Mucuna pruriens, widely grown in the southeastern United States and in Africa, can also find a place in Martinique as a short-term intercrop or in certain intensive vegetable systems to control nematodes, particularly Meloidogyne spp. (Quénéhervé et al. 1998).This third plant, Arachis pintoi, recently introduced by CIRAD-FLHOR into Martinique, seems to offer certain advantages, but also possesses disadvantages:• advantages: commercially available seed, propagation by seed, or vegetative; \"non-host\" plant to several nematode species including Meloidogyne spp.; suitable as a cover crop; supplies nitrogen (about 60 kg/ha/year). • disadvantages: plants are host to serious migratory endoparasites, including R. similis and P. coffeae; slow to establish; requires inoculation with a specific associated bacterium.The introduction and use of Arachis pintoi as a cover crop for bananas could therefore take place under certain conditions:• in the absence of the nematodes R. similis and P. coffeae, which would limit its use immediately after banana or another crop infested by P. coffeae, such as yam or dasheen; • after crop rotation but in the presence of Meloidogyne spp. so as to reduce the infestation potential of these root-knot nematodes before replanting with tissue-cultured banana plants. This plant could also find a place in Martinique and elsewhere in the West Indies in various other ecosystems, which remain to be experimented with:• in orchards such as citrus and especially guavas which suffer serious attacks from M. mayaguensis in the West Indies (Quénéhervé et al. 2001); • as a fallow crop or intercropped cover crop for vegetables. ■Agronomy Micronutrient studiesB oron (B) is the only non-metallic element of the six essential micronutrients; it has a constant valence of +3, and has the smallest ionic radius. It is found mainly in sedimentary rocks. Of the igneous rocks it is most abundant in granites, in the form of borosilicates, with tourmaline (3-4% boron) the most common of the minerals. It is found in soil in four states: a) forming part of the crystalline structure of minerals; b) adsorbed or bound by soil colloids; c) as an anion in the soil solution and d) associated with soil organic matter (Bonilla et al. 1994).The total content of boron in soils varies from 2 to 200 ppm most of which is not taken up by plants. Compared with other micronutrients, boron has several special features, thus in soil solution it is always found in combination with oxygen, behaving as an anion (borate) in all reactions. The borate anion is highly mobile and hence is easily lost by leaching. The available boron in soil can be considered as belonging to a cycle where a small amount originates from tourmaline and a large part from soil organic matter.Organic material is decomposed by microorganisms which liberate available boron to the soil solution, from whence it is taken up by plants; part can be washed out by percolating water, and a small part is fixed or bound by clays (Berger and Pratt, cited by Bonilla et al. 1994).The multiple functions involving boron in plant metabolism include the following: it affects, inter alia, the processes of flowering and fruiting, germination of pollen grains, cell division, cell wall synthesis and the metabolism of nitrogen, carbohydrates and pectic substances. These substances are reported to increase in plants that are deficient in boron (Rajaratnam and Lowry 1974).Another function of boron is the absorption of water by protoplasm and the absorption of mineral salts. The main function of boron is reported to facilitate the transport of highly polar sugar molecules across the cell wall. Boron is a constituent of cell membranes and is immobile in the plant; therefore any boron deficiency is immediately reflected in a change in the metabolism of carbohydrates (which accumulate in leaves). This condition could be the cause of almost all the remaining functions attributed to boron (Gómez and Leguizamón 1975). In spite of the important advances in mineral nutrition, the role of boron in plant metabolism still raises many questions.At present, in the coffee production area of central Colombia, many plantain crops show symptoms associated with boron deficiency. According to León et al. (1985) ten examples of boron deficiency were reported in the country. In the present study we tried to obtain a sound foundation in order to deal more clearly with the problems above. The objective was to determine the importance of boron in the cultivation of plantain (Musa AAB cv. Dominico hartón) in the Quindío, and to study the dynamics over a period of ten years, in a soil fertilized with the major elements.The study plot was located at the Experimental station El Agrado, District of Montenegro, Department of Quindío, Colombia. The station was 1320 m above sea level, with an average rainfall of 2000 mm/annum, a mean annual temperature of 22 o C and a relative humidity of 76%.According to the classification of Holdridge, the ecosystem corresponds to premontane humid woodland. The soils are derived from volcanic ash (andisols) and have an average natural fertility, a medium to heavy texture, a low moisture retention capacity, and are leached and susceptible to erosion.For the study the soils were taken for analysis from 2 May 1990 until 2 March 2000. Soil samples, replicated five times, were taken every two years. Rainfall data was analyzed for the same period.Samples were analyzed for pH, organic matter content, exchangeable calcium, phosphorus (P), magnesium (Mg), potassium (K) and boron (B). The analytical methods are described in Table 1. The data were analyzed to determine the correlation between: boron-weight of bunch (for each production cycle), boron-potassium, boron-calcium, boron-percentage soil organic matter, and boron-pH. The relationships between Ca/Mg, Mg/K, Ca/K and (Ca+Mg)/K were also analyzed.The data obtained from soil analyses, averaged over the five replicates, changed over the years as shown in Table 2.As can be seen from the chemical analyses during the 10 years of the study, boron content declined considerably from levels sufficient for cultivation of plantain, according to Buriticá (1985) from 0.4 ppm to 0.01 ppm boron, a value which gave rise to deficiencies. However, one must consider the edaphic cycle of boron which determines its concentration in soil solution and hence the availability for uptake by plants (Mengel 1980).As can be seen from Table 3, boron showed a direct and close correlation with pH, hence this is in a range optimum for absorption of boron; fixation of this microelement to hydroxides of Fe and Al, as is with clays, increases with pH being a maximum between pH 8 and 9 and a minimum at about pH 5 (Lora 1994). According to Domínguez (1988) the increase in pH reduces the availability of boron but this does not become evident until more than pH 6 which did not occur after the start of the experiment.According to Marschner (1986) the availability of boron to plants decreases with increased soil pH, as happens in calcareous soils or in soils with high clay content, presumably as a result of the formation and absorption of B(OH) 4 .In agreement with the soil chemical analyses, the pH value (5.1-6.08) fluctuated in the range sufficient for the micronutrient to be available. This explains why symptoms of boron deficiency only became evident in the last years. The explanation for the correlation between boron content and pH, are based on the following: • Profoundly influences many biological processes in soil, • Affects the availability of micronutrients, • Alters the absorption of an element and its effect on microbial activity, • Results in changes in the ability of roots to absorb or transport ions once they have been taken up, • Causes variations in the stability of soluble and insoluble organic complexes, • Changes the solubility of antagonistic ions and changes conditions in the rhizosphere.The results also show a close inverse correlation between K and boron (Table 3) explicable because the K content over the years had reached levels greater than 0.3 meq/100 g soil, which, according to Gómez and Leguizamón (1975) can induce boron deficiency.The potassium-boron interaction does not appear to follow a particular pattern. Revé and Shive (1944) cited by Domínguez (1988), demonstrated that in a boron-rich medium, absorption of boron increased as the soil became enriched with K but, in contrast, when boron levels in the medium are low, boron deficiency becomes worse as K increases. The direction of the interaction between K and boron appears to depend on the amount of boron in soil solution. The trend in this study showed that increasing applica- (1990)(1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000). When boron interacts with other elements, it is necessary to consider the possibility of nutritional imbalances in soil, given that this implies an antagonism that affects the plant directly, as one or other elements would not then be available. Such is the situation with potassium that is absorbed in smaller amounts when boron content is very low.As regards calcium, the levels increase when boron is deficient. In the soil under study, where we did not find a high availability of Ca, this could favour boron uptake. However, the interaction calcium-boron has been studied with rather high concentrations, and with the relationship of Ca/boron in the plant. Revé and Shive (1944) cited by Domínguez (1988), indicated that high concentrations of calcium exacerbate symptoms of boron deficiency in tomato. The toxicity of boron, in a medium with too much of the element, can on the other hand have a reduced increase in the amount of calcium in the medium. It is possible that with all the preceding, boron deficiencies in the cultivation of plantain studied, only became apparent in the last years (1999)(2000) even when the low levels of this element were present from 1993.Table 3 shows an inverse correlation of P to boron. According to studies by Robertson and Loughman (1974), it was evident that there was a clear reduction in the uptake of phosphorus in borondeficient plants. This idea is based on the role of boron as a stimulant of the utilisation of glucose1-phosphate. This suggests that when boron is low, the P found in soil will be taken up very slowly, leading to a steady accumulation of P in soil.According to the relationships obtained between the different cations (Table 4) and later comparisons with the critical levels, K was never seen to be deficient, which is explained by the large quantities of potassium containing fertilizers that were applied over the years, as well as by the recycling that occurs with this element in residues from the harvest of the plantain. According to Belalcázar (1991), cultivation of plantain removes a large percentage of elements such as potassium (76.02%) and calcium (13.62%), followed by nitrogen, magnesium and phosphorus. Those with the higher percentage that are removed are nitrogen (25.55%) followed by magnesium (20.09%) and phosphorus (19.80%), whilst those that are reincorporated or recycled in higher amounts are calcium (94.47%) and potassium (89.77%).With reference to magnesium, amongst the factors that gave rise to the deficiency in this macronutrient, were unsuitable relationships with the other bases in the soil, mainly potassium (Table 4).The relationship Mg/K appeared to be unbalanced, explaining a deficiency of Mg. Therefore, the high levels of K function in a manner antagonistic to Mg, implying a low absorption for this element. The losses of magnesium in soil are greater when added with potassium fertilizers. Many authors consider that a soil to be low in magnesium when it has less than 1.0 meq/100 g is present, whilst others classify soils as poor in magnesium when there is less than 1.5 or even 2.0 meq /100 g (Suárez and Carrillo 1984).Intensive and continual fertilisation with K, as employed in this zone, possibly contributes to Mg deficiency, resulting in an imbalance in the relationship Mg/K and as a result an inhibition of Mg uptake. It should be noted that in the zone where this study took place, it is common to come across crops with symptoms of magnesium deficiency. In accordance with Fried and Dean (1952) nutritional deficiencies resulting from an imbalance can be corrected by a programme of balanced fertilisation.In accordance with the results of this study there was no correlation between organic matter and boron, however, it should be noted that various authors (Gómez and Leguizamón 1975) claim that in mineral soils rich in organic matter, boron deficiency is rarely seen because soil organic matter is a major source of boron. Similarly, Berger and Truog (1945) cited by Domínguez (1988), obtained a positive relationship between assimilable boron (water soluble boron) and the organic matter content of soil. More recently, Olsen and Berger (1946) cited by Domínguez (1988), demonstrated that mineralization of organic material leads to a release of assimilable boron.On the other hand, boron absorbed on organic and inorganic soil colloids constitutes a reserve that maintains the concentration of boron in solution; this helps to replenish the demand by crops and reduces losses by washing. Furthermore, soils with higher organic matter content have higher concentrations of boron, since an important fraction of soil boron comes from soil organic matter.There was a close and direct correlation between yield and boron (Table 3). This is explicable as a result of the chemical degradation of the soil, as shown in Table 2. The gradual loss of the micronutrient boron, drastically affects the filling of the fruit (Figure 1) to the point where the young fruits become deformed, mature prematurely and size is reduced, hence yield declines and they are difficult to market. Clearly productive capacity of plantain is harmed. The reduction in yield can also be associated with the regulation of uptake and translocation of boron by the plants, which is more limited in comparison with other minerals. At the same time, the low quantity and quality of production could be caused by an early boron deficiency, which checks growth of the apices and restricts cell elongation (Lovatt et al. 1981, Robertson andLoughman, 1974b) and cell division (Cohen and Lepper 1977).According to Leguizamón (1975) in many situations the affected developing bunches do not produce a yield and in this situation, bunches are small and deformed.From the above it can be concluded that boron is a nutrient fundamental to good production as well as fruit quality and in quantity.Boron content tends to decline as a result of the high rainfall which occurs in the area of the study as shown in Figure 2; this, linked to the sandy loam type texture, and the definite mobility of the boron anion, gave rise to an increased rate in leaching of boron. Thus, the nutrient should be applied in a more finely divided form.This result was in agreement with Marschner (1986) who suggested that under conditions of high rainfall boron is washed out as B(OH) 3 .A general aspect of boron deficiency is the poor development of meristematic tissues, as found at the tips of young roots and in the buds. With boron deficiency, irregularities in development are the first symptoms (Domínguez 1988). This check to the growth of the root tips possibly contributes to one of the main problems of the cultivation of plantain, which harms the plant. Primavessi (2000) confirmed that the addition of boron aids root growth, and that if this continues in the coat with organic matter and roots do not want to penetrate the soil, there may not be sufficient boron.Boron deficiency in plants is not easily identified except by leaf or soil analysis. This is important in the cultivation of plantain because the micronutrient plays a key role in the transport of sugars, as a result of the transformation of boronsugar complexes (Marschner 1986) and therefore affects the filling of the fruits; in such situations, a deficiency directly and adversely affects the quality and quantity of the plantain harvest.• Boron is crucial for optimal yields of plantain, as well as for the quality and quantity of fruit. This was confirmed by the correlation between bunch weight and boron. • The availability of boron in soil solution is closely linked to rainfall and the loose sandy loam texture of the soil and the definite mobility of the borate anion. • In the experimental soil, from 1990 to 2000, as soil potassium content increased so boron content declined, and as a result yields of the experimental crop of plantain declined. This could be associated with the repeated application of potassium fertilizer to the soil.Further investigations are needed to improve accuracy of the recommendation for fertilization with boron in systems of plantain cultivation, in relation to boron content of leaves and based on different critical levels of extraction of the borate anion.P. Orellana Pérez, I. Bermúdez Caraballoso, L. García Rodríguez and N. Veitía Rodríguez P lantains are an important food source for the populations of Latin America and certain African countries. Plantains of the 'Horn' type, traditionally the most commonly grown, are seriously affected by black leaf streak (Mycosphaerella fijiensis Morelet), which has greatly reduced the supply of this product both in local markets and for export. This is the main disease which threatens the production of this source of both food and income (Jacome 1998). The fact that plantains are usually grown on smallholdings, sometimes in mountainous areas and very often interplanted with other crops, makes chemical control of the disease difficult. The consequence, not only on volume of production but also on the quality of the product, is that current levels of production fail to meet the growing demand of certain local and export markets.In the 1990s, the first plantain hybrids resistant to black leaf streak and destined for commercial use, developed by the Fundación Hondureña de Investigación Agrícola (FHIA), offered the possibility of introducing new clones into commercial production and of restoring adequate production levels at lower cost.However, because of their genetic constitution, which includes a contribution from clones of the tall 'French' type, the new hybrids must be characterized morphologically and studied agronomically before being exploited commercially.The work presented here shows the results of evaluation of various agronomic characters of the FHIA hybrids in the central region of Cuba.The studies were made on tissue-cultured plants produced by micropropagation using the method proposed by Orellana (1994), planted out on the multi-crop farm \"La Cuba\", situated in the province of Ciego de Avila.Fifty plants of each hybrid were planted 3 metres apart, in two furrows also situated 3 m apart. On 20 plants of each hybrid, starting at the beginning of flowering, the following characters were recorded: Using the results of counts of the number of functional leaves showing typical black leaf streak lesions, two formulae were devised to serve as indicators of the reduction in functional leaf area: the Functional Leaf Reduction Index (FLRI) and Relative Infection Index (RII), reflecting the damage caused by the disease. The latter index depends on the number of functional leaves showing typical lesions at the start of flowering and when the bunch is harvested.Formulae:As there is only one production cycle in Cuban plantains, the counts all apply to the mother plant.The results show that with the exception of FHIA-19, which has the lightest bunches, the hybrids did not differ in their bunch weight. For all the hybrids, the majority of the weight of the bunch is concentrated in the first four hands (59.71% of the total weight). FHIA-19 had the highest proportion in these hands (71%), which is confirmed by the observation of the length and breadth of the fingers of the first hand (Table 1). The concentration of the majority of the weight in the first hands is a characteristic of the plantain. It justifies the removal of the terminal hands of hybrids that have developed more than eight hands per bunch in order to encourage greater development of the fingers in terms of length and diameter. This latter aspect is very important if the hybrids are to claim to rival the 'Horn' type plantains. No differences were observed between the hybrids as regards other bunch characteristics. Arcila et al. (2000) recommended leaving five hands and removing the rest 20 days after the start of flowering.It is important to emphasize that the hybrids with the longest interval between harvest and ripening under natural conditions (11 days) are FHIA-20 and FHIA-22; for FHIA-21 it is only 8 days. This shows that the first two have advantages for local sale and for export over short distances.As for response to black leaf streak, the FLRI shows that FHIA-04, which has only 1.3 functional leaves at harvest (FLRI = 9.31) is the hybrid whose leaf area was most reduced during the filling process of the fingers, resulting in insufficient filling of these fingers. The other hybrids had lower values of this index and similar for them all (Table 2). For these hybrids, the number of functional leaves at harvest was not less than four, allowing filling of the fingers.The results indicate that hybrid FHIA-04 is also the most affected by black leaf streak, with an RII of 9.31 due to the fact that all its functional leaves bore typical lesions of the disease, which developed rapidly after flowering. At the time of harvest, FHIA-20 and FHIA-22, with more than two functional leaves unaffected by the pathogen, had the lowest values of RII: 1.38 and 1.40 respectively. FHIA-05, FHIA-19 and FHIA-21, although having higher values, responded well to the disease itself even though all their functional leaves at the time of harvest bore typical lesions. The results confirm that the time needed for the development of the disease on FHIA-04 was very much less than on the other hybrids, as already reported by Jones (1994).The results suggest the possibility of using FLRI as an expression of the reduction of leaf area during the process of fill-Performance of plantain hybrids ing of the fingers, and RII as an expression of the time needed for the development of the disease as a function of the leaf area affected, given by the number of functional leaves and that of leaves which are necrotic at harvest time, a relationship which has always been difficult to quantify numerically.According to Ortiz and Vuylsteke (1994), cited by Craenen (1998), it requires at least eight functional leaves during the whole growth period and a similar number of healthy leaves before flowering to guarantee a good yield.From this point of view, FHIA-20 has the shortest growth period from planting to harvest, at 481 days, whereas for the other hybrids this period varies from 493 to 518 days. (Vasil 1994). This tech-nique is the basis of mass propagation of bananas and plantains with the aim, for many countries at the present time, of large-scale commercial distribution of completely disease-free plants (Afza et al. 1996).Genotype is known to have an influence on the efficacy of in vitro propagation and so, when new varieties or hybrid clones are introduced into production programmes, it is necessary to modify the micropropagation techniques used. Banerjee et al. (1986) (cited by Afza et al. 1996) found considerable differences between clones in shoot formation. This seems to be correlated with the presence of one or two B genomes.In vitro propagation of the hybrid FHIA-20 (AAAB) has proved difficult. Conversion of apices into plants has been observed during the initiation phase, as well as buds growing out in the form of rosettes and presenting white bulbous structures during the multiplication phase, resulting in a reduction of the multiplication coefficient. In view of these problems and the need to multiply the hybrid FHIA-20 efficiently in vitro, the development of alternative protocols for the manipulation of apices during the initiation phase, and of axillary buds during the multiplication phase, appear necessary.For the study, young plants with a mean height of 25.6 cm, grown in the greenhouse, were selected (Figure 1). The process of introduction into the laboratory, including manipulation of the plants, disinfection of the corms, culture media for initiation and multiplication, together with the culture conditions, were as described by Orellana (1994).The plants were cultivated in chambers under natural light conditions at a temperature of 27 ± 2ºC. In every case, the base of the apex or bud was placed downwards on the culture medium.This study was made in order to establish the conditions for manipulation and growth of the apex during the initiation phase. For this purpose, the following treatments were studied (Figure 2): 1. 0.5 cm 2 apex cultured in liquid medium (control) 2. 0.5 cm 2 apex cultured in a semi-solid medium 3. 1.0 cm 2 apex cut in halves and cultured in liquid medium 4. 1.0 cm 2 apex cut in halves and cultured in semi-solid medium At the end of 20 days' culture, the following variables were recorded:There were 20 replicates and the statistical method used to compare percent-ages was the comparison of proportions -ANOVA. The analysis of the variable \"number of buds per apex\" was a simple analysis of variance and the comparison of means was made using Tukey's test at P < 0.05%.14.5 x 2.0 cm test tubes were used containing 10 ml of culture medium. For liquid culture media, a paper filter support was used, forming a bridge on which the apices were placed. In the case of semisolid media, 2 mg.L -1 of the gelling agent Gellan gum (Spectrum) was added.The plants obtained during the initiation phase were transferred, after separation and decapitation, onto multiplication culture media. It was observed that the growth of the buds continued throughout this phase as small rosettes and presented white bulbous structures. This behaviour of the buds of FHIA-20 during the multiplication phase resulted in a reduction in the multiplication coefficients (buds obtained/buds initiated).With the aim of resolving problems encountered during the growth of buds in the multiplication phase, the effect of a dose of 2 mg.L -1 of 6-benzylaminopurine (BAP) was studied, the rate of 4 mg.L -1 , proposed by Orellana (1994) serving as control. Each dose was combined with two manipulation protocols.Protocol 1. The buds were separated, decapitated 0.5 cm from the top and cut in two.Protocol 2. Buds which had not reached 1 cm length were left in groups of two or were not separated from the mother plant, and there was no decapitation. Buds of more than 1 cm were separated, decapitated at this height and cut into two when the pseudostem was composed of more than three leaves.Four treatments were thus obtained: 1. Multiplication medium with 4 mg.L -1 BAP combined with protocol 1 (control). 2. Multiplication medium with 4 mg.L -1 BAP combined with protocol 2. 3. Multiplication medium with 2 mg.L -1 BAP combined with protocol 1. 4. Multiplication medium with 4 mg.L -1 BAP combined with protocol 2. The variables recorded were the number of buds per initial explant and the percentage of buds growing as rosettes. The counts were made after three subcultures carried out every 21 days (culture in growth chambers with natural light and a temperature of 27 ± 2ºC).Five explants were inoculated in 250 ml flasks containing 30 ml of semi-solid culture medium (2 mg.L-1 of Gellan gum (Spectrum). There were 10 replicates. The data were analyzed by multifactorial variance and the means compared with Tukey's test. Results in percentages were analyzed as in the previous experiment.Influence of the size of the apex and the physical state of the culture medium during the initiation phaseThe use during the initiation phase of apices of 1 cm 2 , cut in halves and placed on a semi-solid culture medium, gave 85% regeneration after 20 days of in vitro culture. On each section of the apex axillary buds were noted, guaranteeing a larger number of future explants at the multiplication phase, significantly different from that obtained with the other treatments (Table 1).The technique of decapitating the apical dome proved necessary to induce the formation of new buds from the axillary buds, normally inhibited by apical dominance (Ma and Shi (1972) and Swami et al. (1983) (cited by Afza et al. (1996)). Pérez et al. (1998) emphasized the importance of increasing the multiplication coefficient during in vitro propagation of plantains, because each increase of this indicator by one unit corresponds to a cost reduction of about 10%.In this study, mortality occurred only in apices cut and grown in liquid medium, possibly because the cutting procedure produced pieces which were too small to be grown in a liquid medium (Table 1). According to Orellana (1998), there are differences in tissue growth depending on the physical state of the culture medium: it proceeds differently according to whether the medium is solid or liquid.The incidence of infection during this phase did not differ significantly with the treatment. However, various authors mention the influence of the size of the initial explant on the incidence of infection and have noticed that the smaller the explant, and the closer it is located to the apical meristem, the more the populations of micro-organisms fall (Garcia and Noa 1998, Leifert et al. 1994).By reducing the dose of cytokinin in the multiplication medium, the differentiation of buds into plantlets could be stimulated whilst little by little the growth of rosettes disappeared (inasmuch as the three subcultures were made with the 2 mg.L -1 dose of BAP). The manipulations or cuts made during this phase, combined with the reduction in the dose of cytokinin encouraged the biological response of the plants and caused the appearance of a larger number of buds per initial explant when using protocol 2 (Table 2). These buds, once transferred onto rooting media, had no difficulty in continuing their growth and reached the height, girth, and number of leaves needed to allow their transfer to the acclimatization phase.On the other hand, for the treatments receiving the 4 mg.L -1 dose of BAP, we continued to note the appearance of rosette growth for both protocols, although the highest percentage of this type of growth occurred with the normal protocol (i.e. separation, decapitation at 0.5 cm and cutting buds into two). It seems that this treatment accentuates the presence of this particular growth in the clone FHIA-20: in fact, the presence of rosettes tended to diminish when protocol 2 was used (Table 2).The development of cultures in vitro requires an adequate ratio between auxins and cytokinins in the culture medium. One must also consider the endogenous concentrations of these hormones in the different types of explants or species (Jiménez 1998). Certain species are cultivated without the addition of any external regulator, probably because there is enough endogenous hormone present.The results obtained from this work make it possible to propagate the hybrid FHIA-20 in vitro with a distinct improvement in the efficiency of the process of propagation by organogenesis by virtue of an increase in the number of buds. During the initiation phase it is necessary to culture apices of 1 cm 2 , cut into two, on a semi-solid medium. In this way, 85% of them regenerate plants by the end of 20 days' culture. During the multiplication phase, the cytokinin dose should be reduced to 2 mg. L-1 in the culture medium and the explants separated into welldefined buds which are not less than 1 cm tall (those which are should be kept as pairs or should remain on the mother plant). Buds of 1.5-3.0 cm having more than three leaves can be decapitated at a height of 1.0 cm and cut in half. This way rosette growth is reduced by 2% and on average 4.7 buds per explant are obtained in the multiplication phase. ■ 2. 0.5 cm 2 apex cultured in semi-solid medium.3. 1.0 cm 2 apex cut in two and cultured in liquid medium.4. 1.0 cm 2 apex cut in two and cultured in semi-solid medium. T he production of bananas and plantains is widely distributed in tropical and sub-tropical regions. The area cultivated, estimated to be around 10 million hectares, gives an annual yield in the order of 88 million tonnes. This crop, whose fruit forms part of the diet of more than 400 million people, ranks fourth in the world in the category of staple food products, after rice, wheat and milk (FAO 1999).In view of the interest in banana growing, considerable research effort has been devoted to the improvement and control of its mass propagation by means of biotechnological techniques such as somatic embryogenesis, for which three protocols have been described using vegetative tissue such as fragments of the corm and leaf bases (Novak et al. 1989, Ganapathi et al. 1999), cultures of proliferating meristems (Dhed'a et al. 1991, Dhed'a 1992, Schoofs 1997, Schoofs et al. 1998), and immature male or female flowers (Escalant et al. 1994, Grapin et al. 1996).The use of cell suspensions in somatic embryogenesis and the discovery of the factors involved in the metabolic synchronization of cell suspensions and its timing constitute two fundamental aspects either of the procedures for applying methods of temporary immersion for mass micropropagation of economically important plant material (Escalant et al. 1994, Gómez-Kosky et al. 2000), or of their use in genetic improvement programmes by the induction of mutations, the study of selections in vitro (by means of fungal toxins or plant extracts) and genetic transformation by particle bombardment. Despite all the research carried out internationally in various laboratories, it is still difficult to maintain cell suspensions effectively. The setting up of banana cell cultures free from bacterial contamination, changes due to oxidation or possible fungal attacks requires a lot of time, and their maintenance therefore proves to be difficult (Schoofs et al. 1999). The aim of the work presented here was to determine, by using sources of carbon and growth regulators, the optimum experimental conditions for the setup and multiplication of a cell suspension on the one hand, and for the regeneration of the somatic embryos on the other.The plant material used to initiate the cell suspensions consisted of immature male flowers of Musa AAA cv \"Grande naine\" which had been placed on M1 induction medium [Murashige & Skoog (1962) salts -MS, 1 mg/L biotin, ANA and AIA, 4 mg/L 2,4-D, 6 g/L agarose, 30 g/L saccharose, pH 5.71] proposed by Grapin et al. (1998) for forming calluses. The friable embryogenic tissue obtained was transferred into M2 cell suspension medium [MS salts, 100 mg/L glutamine and malt extract, 1 mg/L 2,4-D, 45 g/L saccharose, pH 5.3] until its establishment. This technique for somatic embryogenesis was originally developed by Escalant et al. (1994) and it is currently applied in the biotechnology laboratory of CORBANA on this same clone (Acuña and Sandoval 2000).From this initial suspension, new cultures were started during the phase of maintenance in M2 medium onto a medium made up of 35 ml of fresh M2 medium and 13 ml of the previous M2 medium (in which the suspension had been maintained during the preceding cycle), a mixture into which were introduced 2 ml of cells made up to a total volume of 50 ml per erlenmeyer flask. These suspensions were subjected to four treatments: T0 = 45 g saccharose; T1 = 45 g saccharose + 100 mg/L myoinositol; T2 = 30 g saccharose + 100 mg/L myoinositol; T3 = 15 g saccharose + 100 mg/L myoinositol. There were 10 replicates (Figure 1).Four subcultures were made which were each incubated for 14 days as proposed by Escalant et al. (1994). The number of cells and the percentage viability of the suspensions were recorded on the 1 st , 7 th , and 14 th days of the culture using a haemocytometer. There were three replicates and 5 counts for each, making a total of 15 readings per treatment. Also, every 15 days, the increase in cell volume was measured by the sedimentation method (SCV) proposed by Schoof (1997) and the packed cell volume (PCV) as used by Reinert and Yeoman (1982). Four extra replicates were also made for monitoring the pH (2 in the inoculated medium and 2 in the non-inoculated medium), the measurements being made at the beginning and end of each subculture.In order to evaluate the effect of the growth regulators on the quality of the cell suspension in the M2 medium, the treatment was selected which showed the highest multiplication rate and cell viability during the first four subcultures of the maintenance phase. For this study, we added to the selected M2 medium: A1 = 0.5 mg/L 2,4-D, A2 = 1 mg/L 2,4-D and A3 = 2 mg/L 2,4-D. The material was handled the same way as the treatments involving different saccharose concentrations. For evaluation, the same parameters were used as in the maintenance phase of the cell suspensions (mentioned above). The cell morphology was also noted, as clusters or solid masses, and photographs were taken using both optical and electron microscopes.The viability of the process was evaluated by observing the embryos obtained on the culture medium of Schenk and Hildebrandt (1972), called modified M3 [10 mg/L biotin, 100 mg/L of glutamine and malt extract, 230 mg/L proline, 1 mg/L ANA, zeatin and 2-IP, 10 g/L lactose, 45 g/L saccharose and a pH of 5.3]. The M3 medium was put into Petri dishes and sterile filter papers were placed on the surface on which were inoculated aliquots of 1 ml of the cells of treatments corresponding to the different growth regulator concentrations. The type of material regenerated was evaluated by making three evaluations per Petri dish from the zones where the distribution of the suspension was most homogeneous. All the cultures were maintained under conditions of controlled temperature (27ºC), relative humidity (80%) and photoperiod (12 hours).The results obtained for the phase of maintenance of cell suspensions and homogenization of cultures for variation in pH, cell volume, number of cells and percentage viability, were analyzed by a linear modelling scheme and subjected to analysis of variance using the SAS programme (1990). Results showing heterogeneity of variance were adjusted to homogeneity by using a square root transformation.The results for the increase in numbers of cells, presented in Figure 2, indicate that the dose of 30 g of saccharose provides enough carbon for the suspension, as its behaviour differed little from that of the suspension maintained with 45 g of saccharose. In general, the addition of myoinositol (T1-T2) did not alter the behaviour of the cells and a tendency was noted to stabilization in subculture 4 (relation of T1 and T2 with T10).There were no significant differences between the percentages of viability of the treatments with and without myoinositol (T1 and T0 respectively). No differences were noted between the observations at different times (7 or 14 days) nor any interactions between the subcultures and time of observation (P = 0.1574).On the other hand, as for the behaviour of this same percentage viability for treatments with myoinositol associated with different saccharose concentrations (T1 and T2), it was found that the differences for the four subcultures depended on the treatment (P = 0.0040). This difference in behaviour of distinct cell lines of a single clone may be an intrinsic characteristic of the material (Schoofs et al. 1999), which would indicate the need to redouble efforts towards improving these methods.Treatment T3 (15 g saccharose + myoinositol) was eliminated because it showed progressive diminution of 5.18, 4.20 and 2.06 ml in subcultures 1,2 and 3 respectively. This low success rate of cell proliferation may be attributable to the low availability of sugars in the medium compared with the demand of the cells in phase G1 of the cell cycle or to the osmotic shock due to the medium. Whichever it is, it is well known that saccharose as a source of carbon is a stabilizer of culture media (Takeuchi and Komamine 1982, Vardi et al. 1982, Smith et al. 1984).The differences in cell volume found between treatments T0, T1 and T2 (P = 0.02602) were much reduced in subcultures 1 and 2 (Figure 3a and b) but were accentuated in subcultures 3 and 4 (Figure 3c and d) for which the difference between treatments T0 and T1 can be attributed to the action of myoinositol. Subcultures 2, 3 and 4 of T1 (with myoinositol) produced a mean cell volume 0.67 ml greater (P = 0.0188) than that reached in the T0 subculture homologues. These results agree with those from other research on banana and other crops: for example Cronauer and Krikorian (1983) and Aftab et al. (1999) confirm the stimulatory action of myoinositol on mitosis and morphogenesis of plant cells. The difference between the four subcultures was on average 0.23 ml in favour of treatment 1. The treatment which responded best was that containing 45 g saccharose and 100 mg/L myoinositol. It was also noted that the cell volume of subculture 1 was 5.95 ml and that of subculture 4 was 7.59 ml, representing a mean increase of 0.65 ml for each. There is a positive correlation (Figure 3e) between the number of subcultures and the cell volume, since this latter increases with the number of subcultures, finally to stabilize at the fourth subculture.After mixing, the 35 ml of fresh medium and the 13 ml of old medium had a pH of 4.74. During the 14 days of culture, the non-inoculated media remained between pH 4.1 and 4.2 and the inoculated media between 4.4 and 4.6 (unpublished results). In the cell suspensions, the pH varied with time, treatment and their interaction (P = 0.0001): similar behaviour was observed by Skirvin et al. (1986). These same authors suggested that acidification of the medium could be due to ionic exchange between the cell and the culture medium, leading to an optimum pH for the normal functioning of the cell wall.Analysis of the results obtained for the variables \"number of cells\" and \"percentage viability\" on media containing different concentrations of 2,4-D did not show any marked differences in their behaviour. Treatments A1, A2 and A3 of the four subcultures had mean numbers of cells of 7.9, 6.0 and 7.0 and percentage viabilities of 59, 62 and 59 respectively.When one studies the cell volume obtained with varying concentrations of 2,4-D (Figure 4) it is seen that treatment A1 (1 mg/L of 2,4-D) is that which maintains the best cell suspension with a mean volume of 7.6 ml and a maximum of 8.8 ml in subculture 3. The 2 mg/L dose of 2,4-D was the best for standardizing the cell volume of several subcultures, which is a useful parameter for carrying out studies of the cell cycle or cell metabolism and other phenomena connected with synchronized cell populations.The final results from subculture 4 measured by the PCV method show that all the treatments have progressively increased the cell volume without large fluctuations during the 14 days of incubation and that this volume doubled on the sixth day, when the cells begin a phase of active division (Figure 5). These results agree with those obtained by Bieberach (1995) for various Musa clones.As to the use of 2,4-D and appropriate doses, the results given here supplement the information about the action of this growth regulator on the embryogenic process and the doses required by different plant species. Lazzeri et al. (1987) emphasize the importance of auxins in the regulation of the somatic embryogenesis of soyabean and show that there is better somatic embryo production when 2,4-D is used alone than in combination with acetic a-naphthalene.The morphology of cells in suspension was observed by optical microscopy at magnifications of 20x and 40x. The preparations showed cell clusters and isolated cells (Figures 6a and 6b), which conforms with descriptions by Grapin (1996) who reported that in suspensions of 'French Sombre' clusters were seen which could reach 70 to 80% of the volume of the suspension very similar to what we observed in the course of this work. The clusters are formed by preembryogenic cells (Figure 6c) possessing partitions or cellular plates typical of the last stage of mitosis and of cells which are empty or in the course of differentiation.The isolated cells are round, with a dense cytoplasm and a well-defined nucleus: one may regard them as proto- Treatments A1, A2 and A3 plasts, initial cells with a primary cell wall that is characteristic of undifferentiated cells and with an active cell cycle. These observations are shared both by Bieberach (1995) who noted the presence of cells with identical morphological characteristics in cell suspensions of the cultivars \"Dominico\", \"Grande naine\"and \"Gros Michel\" and also by Sannasgala (1989) who described pre-embryos made up of protein bodies and starch. The characters described above are a factor indicating the embryogenic condition of the cell suspension (Williams and Maheswaren 1986). Certain isolated cells with elongated cytoplasm containing lacunae are non-viable cells in a suspension because they have already formed their secondary cell wall.Under the scanning electron microscope, round cells were seen of 50-80µm diameter, with rough walls with irregular ornamentations surrounded by a polysaccharide mucus (Figures 7a and 7b).Mixtures of cell samples treated with growth regulators were inoculated and maintained for 55 days on the semi-solid M3 medium to develop embryos. By the end of 22 days their growth began to be apparent, without traces of oxidation. The presence was detected of small clusters of 1 cm 2 containing globular embryos in the shape of a heart or torpedo. The embryos were sorted with a view to later regeneration of the plants (Figure 8a). A total of 200 \"torpedo-type\" embryos were transferred into eight Petri dishes at the rate of 25 embryos per dish. At the end of 20 days there was 63% germination, and after 41 days the plants possessed normal morphological characteristics. Until then the percentage germination for somatic embryos of the genus Musa oscillated between 45% and 80% according to the genotype and culture medium (Bieberach 1995, Escalant et al. 1995, Côte et al. 1996, Schoof 1997, Grapin et al. 1998). Figure 8b shows the potential regeneration of somatic embryos from cell suspensions. Escalant et al. (1994) have obtained the highest germination percentages, achieved by using temporary immersion systems with other banana cultivars.These experiments have enabled us to standardize a protocol for obtaining embryos of Musa AAA cv. \"Grande naine\" from cell suspensions and by using growth regulators. The initial cell suspension was maintained with 45 g saccharose + 100 mg/L myoinositol. An initial pH of 4.74 and four subcultures of 14 days each guaranteed a sufficient cell volume and embryos with a good percentage viability.The optimal dose of growth regulator for the efficacy of the process is 1 mg/L of 2,4-D applied as an exogenous hormone.The morphological observations reveal that the protocol has allowed the development of viable cells which are easily transformed into embryos. The germination of embryos has validated the entire method and the doses used. Unlike most other Central-American countries, the production of banana and plantain in Nicaragua is low (Table 1). The major production zones of bananas and plantains are located in the coastal area near the Pacific Ocean. In the Chinandega region (North-West) an estimated area of 2000 ha Cavendish (Musa cv. AAA) is grown for export, while in the Rivas region (South of Managua) 13 000 ha plantain are cultivated for local consumption. For many small-and medium-scale farmers in Rivas, plantain is the most important crop. Gros Michel (Musa cv. AAA), Bluggoe (Musa cv. ABB) and Silk (Musa cv. AAB) bananas are cultivated all over the country, mainly by small-scale farmers in backyards. In the higher regions of Central Nicaragua, with altitudes up to 1300 masl, bananas are grown in combination with coffee or cacao. Bananas and plantains are also important for the people at the Atlantic Coast. Pelipita (Musa cv. ABB) and Red (Musa cv. AAA) bananas are found in some regions of Nicaragua. Plantain (Musa cv. AAB) is most preferred because it is an attractive cash crop. The most common landraces are False horn plantain with an average of only 25 fingers. The price of plantain on the local market is much higher than the price of the other bananas (Gros Michel, Bluggoe, Silk) because of its much larger finger size and longer green life. Cavendish only enters the local market as after being rejected on the export plantations. Its price is even lower than that of Gros Michel. Over the last five years prices of plantain have continued to increase, which reflects the high demand and insufficient supply of bananas and plantains caused by poor cultivation practices, drought and pests and diseases.Diseases and pests are the main problems; black Sigatoka (Mourichon et al. 1997) and plant material contaminated by weevils (Gold and Messiaen 2000) are the major constraints affecting the small-scale plantain producer. Another important problem, especially in the Leon-Chinandega region, is the uneven distribution of annual rainfall. Without irrigation banana and plantain yields are reduced due to the long dry season.The objective of the intervention is to contribute to food security and food quality of resource-poor farmers by giv- were built with about 20 national and international organizations operating in Nicaragua (Table 3), to accelerate the distribution of improved plants and technologies, and to obtain a maximum feedback from the farmers.The project started in mid-1996. Rooted plantlets were sent by KULeuven for weaning in the small nursery of the UNAN farm in Leon, located a few kilometres from Leon City centre. These plants were used for the first test plots at the University farm.The tissue culture laboratory of UNAN was built in 1997. Tissue culture techniques were transferred from KULeuven to UNAN that produced plantlets to extend the test fields at the University farm. In cooperation with the Centro de Enseñanza Técnica Agropecuaria (CETA), workshops were organized in six communities in Chinandega.In 1998, five extension brochures in cartoon style were developed for distribution to participating farmers (Figure 2). At the test farm of UNAN, a field collection was established containing both the introduced and the locally grown varieties (40 in total), and 2 plots of 36 plants of each variety were evaluated (Table 2).In 1999, two trained Nicaraguan technicians of the tissue culture laboratory produced 6500 plants. One hundred and forty new test plots were planted in the northwestern region of Nicaragua, mostly in Chinandega because of the cooperation with CETA and the larger agriculture activity of that region.In 2000, 20 000 plants have been distributed to 370 new farmers, comprising farmers of the Leon region as well (Figure 1). Ten thousands plantlets were imported from KULeuven to accelerate the distribution of the new varieties. Oxfam-Belgium contracted the UNAN to distribute 25 000 plants of superior varieties to nearly 1000 families that were relocated after the hurricane Mitch in October 1998 and urgently needed new plant material. Therefore the shade house was extended to 700 m 2 .In 2001, a few test fields were also planted in the Rivas, central Nicaragua and Atlantic coast regions, where a selection of farmers received in vitro banana and plantain plants.The number of plants produced and distributed by UNAN's tissue culture laboratory increased from 2000 in 1998 to 15 000 in 2001. The number of farmers participating also increased considerably -from 40 at the start of the project in 1998, to a total of 820 having received A total of 2757 farmers were trained in different workshops and 1500 brochures dealing with field selection and preparation and plant material were distributed. Workshops for farmers were organized in collaboration with local NGOs, governmental and international organizations, which drastically increased the contact with farmers (Table 3). The project also participated in the organization of regional and national workshops for extension workers. Six new brochures were developed about diseases and pest control. A catalogue of the new accessions following the format of Musalogue (Daniells et al. 2001) was made available.Close contact is being maintained with the farmers who are growing the new varieties (Figure 3) to assess their reaction and improve the efficiency of the intervention. Interviews are conducted to determine the acceptance rate of the new varieties and to identify the underlying reasons, e.g. appearance, taste, cultivation as a cash crop and/or food crop, etc. (Table 4). The most popular variety so far is FHIA-03, because of its drought resistance and large bunches that are comparable to the local Bluggoe cooking banana. Tasting sessions are organized on a regular basis and the new varieties are prepared according to prevailing Nicaraguan customs i.e. fried, green and ripe plantains, plantain chips, cooked green and ripe plantain, and banana as a dessert. People are asked to compare the new fruits with the local fruits (False horn plantain, Bluggoe or Silk). First results confirm the acceptability of most varieties but also show that palatability tests are absolutely necessary since visual aspects can determine consumers (Table 5).Currently the laboratory has the capacity to produce 50 000 in vitro plants per annum. Plans are being made to further upscale the production capacity to ensure the sustainability of the tissue culture laboratory by selling the plant material. Small-scale farmers will receive plant material at subsidized prices while commercial growers will have to pay a higher price.The best accepted banana varieties will be produced in large amounts as well as other food crops for which a demand exists in Nicaragua. Distribution and extension work will be increasingly coordinated by local organizations and NGOs. To facilitate this, the UNAN/VVOB staff participated in 2001 in the foundation of a national Musa network, MUSANIC.A baseline study about the socioeconomic situation of the collaborating farmers has been carried out to be able to measure the project-impact within a few years. (Simmonds and Weatherup 1990). Therefore, a wide variety of classification and identification systems exists for banana. Up to now, the traditional classification and identification have been based only on morphology and quantitative traits. Recently, molecular markers have been used to study diversity on plants, animals and microorganisms.The random amplified polymorphic DNA (RAPD) technique, which utilizes polymerase chain reaction (PCR) amplification with single primers of arbitrary nucleotide sequence, has been developed by Williams et al. (1990) and Welsh and McClelland (1990) to produce molecular markers for genetic analysis. RAPDs have been shown to be useful in genetic fingerprinting (Yang and Quiros 1993, Orozco-Castillo et al. 1994, Lanham et al. 1995). In this study, we have used RAPDs for identifying and classifying some banana cultivars.In this study, six indigenous banana cultivars of Vietnam (Table 1), which were obtained from the Institute of Agricultural Genetics (Vietnam) were screened for RAPD markers.DNA was isolated from banana leaves using the method of Murray and Thompson (1980) with some modifications. Four grams of fresh leaf material were grounded in liquid nitrogen in presence of glass-sand. The powdered leaf tissue was stored at -20 °C for two hours. Ten milliliters of extraction buffer [1,5% cetyltrimethylammonium bromide (CTAB); 100 mM Tris-HCl) (pH 8); 20mM ethylenediaminetetraacetic acid (EDTA) (pH 8); 1.4 mM NaCl; 0.2% mercaptoethanol] heated to 65 °C was added, and the mixture was then incubated at 65 °C for 30 min. The mixture was shaken lightly with 1.5 volume of chloroform: isoamyl (24:1) for 20 min at room temperature. The sediment was removed by centrifugation at 3000 rpm for 20 min. The DNA was precipitated by the addition of 0.8 volume of freezing propanol (or 1.5 volume of 96% ethanol). The pellet was washed 2-3 times in 70% ethanol. At the end, DNA was redissolved in a minimum volume of TE (about 200 ml).Twelve primers from Operon Technologies, each ten bases in length, were used to amplify the DNA (Table 2). PCR was carried out in 25 ml reactions containing 20 ng of template (genome DNA), 200 mM of each dNTP, 2.5 units of Taq-polymerase, 15 ng of primers, 10 mM Tris-HCl (pH 8.3), 50 mM KCl, 1.5mM MgCl 2 , 0.001% (w/v) gelatine and 20 ml of mineral oil overlay. Forty-five amplification cycles were performed, each consisting of 94 °C for 30 s, 36°C for 1 min, 72°C for 2 min. Products were analyzed by electrophoresis in 1.1% agarose gels at 100 V for 3 h, stained with 0.01% ethidium bromide and photographed under UV light.• Coefficients of similarity among cultivars were calculated by using the formula of Nei and Li (1979):N i + N j where: N ij = number of bands in common between cultivars i and j, and N i and N j = number of bands for cultivars i and j, respectively.• The dendogram of cultivars studied was produced using the NTsyspc 2.0 computer programme.Twelve primers were used to amplify the banana genome DNA. Nine of them amplified to give multiple PCR amplification products (Figure 1: example with primer H08), and three primers (G6, Y14, Y15) did not. There were two kinds of bands: monomorphic bands which were present in all cultivars and polymorphic bands which were asynchronously present or absent in all cultivars. Nine primers were amplified into 79 bands, of which 67 bands (84.81%) were polymorphic and 12 bands (15.19%) monomorphic. The high proportion of polymorphic bands was due to the very different origin of the cultivars studied. Two primers (D07, G14) amplified 5 bands only, while H07 amplified 17 bands. The size of the bands ranged from 360 Kb to 3200 Kb.The Nei and Li formula allowed to calculate coefficients of similarity among cultivars based on RAPD data. Similarity coefficients reflected the relationship between cultivars. Similarity coefficients between the original cultivars from M. acuminata ranged between 0.764-0.826, while those between the original cultivars from M. balbisiana ranged between 0.696-0.835 (Table 3). Cultivars belonging to the two groups had low similarity coefficients, ranging between 0.317 and 0.461.Specific RAPD markers are bands that are present only in one cultivar. In this study, we found 12 specific markers for 4 cultivars (Table 4). These results suggest that RAPDs can be used for the selection of banana breeds in agriculture. Using RAPD technique for identifying and classifying some banana cultivars in VietnamBased on RAPD data, a phylogenetic tree of banana cultivars was constructed using the NTsyspc 2.0 program. The phylogenetic tree had two branches: the cultivars originating from M. acuminata were on one branch, and the cultivars originating from M. balbisiana were on the other branch (Figure 2). These results are in agreement with the cytological analysis of these banana cultivars. n Nigeria, banana and plantain have always been very important traditional staple foods for both rural and urban population. They serve as a source of revenue for smallholders who produce them at the compound farms, mixedcropping farms and small-scale sole cropping farms (Baiyeri 1996, Ajayi andBaiyeri 1999).Nsukka Urban is heavily populated. It has a large market centre, operated on a daily basis. Men, women and the youths within Nsukka Urban and neighbouring communities converge at the market centre to buy and sell. Agricultural products such as banana, plantain, vegetables, pepper, mangoes, palm oil, other fruits, honey, yam, livestock, etc., are sold. In the area, banana and plantain are found in compound farms and they are mixed with other crops. Each of the banana and/or plantain growers in the area has less than 50 stands and a greater proportion of them grow more banana than plantain (Baiyeri and Ajayi 2000). However, banana and plantain marketing is most prominent among women, especially within Nsukka Urban, University campus and the neighbouring communities. The sale of banana and plantain provides means of livelihood for many households in the area.In view of the significant contributions of banana and plantain to the economic, health and nutritional well-being of both rural and urban households in Nigeria, it is very important that efforts should be made continually to improved their marketing and consumption patterns. In planning such a national banana and plantain marketing and consumption improvement programme, data based on the consumption and expenditure patterns of their consumers in both rural and urban areas would be necessary. The role of Agricultural Extension (AE) in the gathering of data, planning, implementation, monitoring and evaluation of such a programme cannot be overemphasized. AE is a primary process through which the households can learn the reasons for change, the value of change, the results that can be achieved, the process through which change is achieved, and the uncertainties inherent in change (Williams 1978).Expenditure patterns of households in Nigeria vary from place to place. Apart from the income of the households, factors such as preference of a particular product by a member of the household, quality and quantity of the product sold, environment under which the product was processed and sold, and the relative price of the products, also influence the expenditure patterns of the households (Anyanwu 1985).Food consumption pattern, in a broad sense, means not only what the people eat or consume, but also the quantities as well as the forms in which these foods are consumed (Dury et al. 1999). According to Olagoke (1989), food consumption patterns vary from one place to another due to factors such as household size, educational levels of members of the household, relative prices of the food items, environment in which the consumers are living, social values attached to some food items, nutritive values of the food items, type or status of job performed by members of the household, household's tastes and preferences, season/period of the year, and culture/religion of the household members.The study was designed to assess the consumption and expenditure patterns of banana and plantain consumers in Nsukka Urban in Enugu State, Nigeria. Specifically, the study was designed to: Out of the above-listed 11 clusters, five were selected through simple random sampling. From each of the five clusters, 12 households were selected, using clustering and simple random sampling techniques. In all, a total of 60 households were involved in the study, and the head of each of the households was interviewed. A structured questionnaire schedule was developed and used in obtaining relevant information from the consumers of bananas and plantains. The data collected were analysed through the use of percentage distribution and bar charts.Consumption and expenditure patterns of banana and plantain among households in Nsukka Urban are presented in the following figures and tables:Figure 1 shows that the consumption rate of bananas is higher than the consumption of plantains.Most of the consumers depend on the market for their banana and plantain supply. A very small proportion of consumers produce their fruits regularly (Figure 2).It clearly appears in Figure 3 that people prefer to eat plantain (consumed boiled, roasted or fried) in the morning and at night, and banana as a 'snack' in the afternoon.Respondents preferred fried plantain for breakfast. For lunch, pounded and roasted plantains are the most eaten. For dinner, plantain accompanied with rice, beans or yams is preferred (Table1).It is to be noted that plantains are more expensive than bananas (N12 1 or N15 per plantain finger and N5 per banana finger). This could explain why the majority of consumers surveyed buy banana more regularly than plantain (Figure 4).Most of the respondents (Figure 5) spend only 1% of their income on bananas and plantains. The main factors that determine the percentage of their monthly income spent on the purchase of bananas and plantains are the availability of money, closely followed by the family interest, and then the price of the fruits (Figure 6 -NB: more than one factor was given).Bananas are mostly purchased ripe, whereas the respondents prefer to buy unripe plantains (Figure 7).Table 2 shows that most of the respondents do not change their habits in buying bananas when the price increases, but they buy more if the price decreases. In the case of plantain, more than half of the respondents would buy fewer plantains in case of increased price and 75% would buy more in case of decreased price.According to Table 3, wives play the most important decision-making role in banana and plantain purchasing and consumption process. On the other hand, the husband plays the major role in the utilization of the peels, while children play the greatest role in determining the purchasing interval and storage period.Three major problems militating against the effective consumption of banana and plantain fruits were identified by the respondents (Figure 8). These included storage problems such as pest (house rats and insects) attacks, over-ripening and mould formation due to sustained bruises; processing problems such as lack The analysis of these results leads to the following conclusions: 1. the consumption rate of bananas was higher than that of plantain; 2. a greater proportion of the respondents depended on the market for their supply of bananas and plantains; 3. bananas were consumed mostly in the afternoon, while plantains were mostly consumed in the morning; 4. plantain meals were prepared and consumed in various forms; 5. plantains were more expensive than bananas; 6. the major factors that determined the proportion (%) of the monthly income spent on bananas and plantains included availability of physical cash, family interest, price, quality and availability of fruits; 7. bananas were mostly purchased in a ripe form, while plantains were mostly purchased unripe; 8. households responded accordingly to change in banana and plantain prices; 9. wives played the greatest decisionmaking role in banana and plantain purchasing and consumption processes than their husbands and children; and 10.the major problems militating against effective consumption of bananas and plantains in the area included storage, processing and transportation problems.1. Since fluctuation in the market price of bananas and plantains affected the consumption patterns of the households, there is a need for the establishment of a consumer cooperative organization for bulk purchase and retail sale of the fruits. Capable extension agents should be attached to each of the so formed organizations for the purpose of monitoring and evaluating the activities of members and giving relevant pieces of information if and when necessary. Iris Engelborghs B anana plants are the most important world fruit crop and show a large diversity in shapes and sizes, of which one is the dwarf type. Unlike the normal type, the dwarf variant has a shorter pseudostem and wider leafs. Because these plants have the same number of leaves as the normal variant, their photosynthetic ability is not reduced and therefore the bunch size almost identical. In addition, its reduced height prevents it from toppling during tropical storms. These characteristics make the dwarf variant a valuable plant for the tropical banana farmer. Different naturally occurring dwarf varieties exist which have a normal variant, but this phenotype is often obtained by in vitro culture too, e.g. in an in vitro germplasm collection or during rapid in vitro multiplication.This DNA fingerprinting technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA and involves three steps: (i) restriction of the DNA and ligation of oligonucleotide adapters, (ii) selective amplification of sets of restriction fragments, and (iii) gel analysis of the amplified fragments. With this method, sets of restriction fragments are visualized by PCR without knowledge of nucleotide sequence. The power of this DNA fingerprinting technique was assessed on a few world species, but not on Musa spp. Therefore, it was here first optimized for banana. In addition, the technique was adapted for non-radioactive detection of the AFLP patterns using a more recent detection method. Fluorescein labelled primers were used in the PCR reactions which allow the computer based separation and detection on a sequencing gel.The assessment of the power of the AFLP technique and its variants threeendonuclease-(TE)-AFLP, cDNA-AFLP and the methylation sensitive amplified polymorphism (MSAP) technique for the characterization and the early detection of the dwarf type was performed in this study on a range of dwarf-normal banana pairs. The dwarf 'Curare enano' and its in vitro generated normal-sized off-type were first used for the optimization of the AFLP technique. Later, the analysis was extended to three naturally occurring dwarf varieties 'Cachaco enano', 'Figue rose naine' and 'Prata ana', which have a normal variant called 'Cachaco', 'Figue rose' and 'Prata', respectively. In addition, the extra dwarf 'Dwarf parfitt', the normal 'Cavendish' and the giant 'Giant cavendish' were analyzed.Differential AFLP, TE-AFLP, cDNA-AFLP, cDNA-TE-AFLP and MSAP patterns were obtained and different levels of polymorphisms were observed between the dwarf and normal type depending on the technique, the primer combination and the variety. For each variety, a distinction could be made between the dwarf and the normal type. However, no dwarf specific fragment was found to be common for all dwarf varieties, which is an indication that (i) either the observed polymorphisms are not related to the phenotype, (ii) or the different varieties originated in a different way, (iii) or that several genes are involved in the process. Differential fragments were cloned and sequenced. Primers were designed on the obtained sequences and used with genomic DNA of the respective variety to confirm the differential and unique nature of the fragments. However, specificity was lost.Besides the above-described analyses at the DNA level, some physiological assays were performed on the dwarf-normal banana pairs. The relation between dwarfism and gibberellic acid (GA) is described for several mutant dwarf species, e.g. rice and wheat, as well as some Musa spp. Two categories of dwarf types are described, i.e. a GA-(in)sensitive group and a GA-deficient group. The influence of GA was tested in this study on the in vitro growth of the dwarf-normal banana pairs. The (in)sensitivity appeared to be variety dependent, suggesting that the different dwarf varieties originated in a different manner and that other mechanisms may be involved than the GA pathway alone. When grown on ancymidol (a GA-synthesis inhibitor) in vitro growth of all the tested dwarf varieties was inhibited, which indicates that these plants are not GA-deficient, and the signal transduction after the synthesis must be impaired.From these results we can conclude that the AFLP technique allows a fast and early fingerprinting of these particular dwarf banana types, that the mechanism behind dwarfism is complex and seems to involve gibberellic acid, (de)methyla-tion… and that the dwarf varieties here analyzed probably originated via different mechanisms. ■ Annemie Elsen D iseases and pests are the major constraints to the productivity of bananas and plantains. Nematodes cause important yield losses in Latin America, West and East Africa and Asia. Usually, banana nematodes are controlled by nematicides. These are not only very expensive but also extremely toxic for non-target organisms, including the user, and they pollute the environment. Arbuscular mycorrhizal fungi (AMF) are obligate symbionts that biotrophically colonize the root cortex and develop an extramatrical mycelium which helps the plant to acquire water and mineral nutrients from the soil, in exchange for carbon as an energy source. In addition, AMF increase the ability of the plant to control the spread of soil-borne pathogens. In Musa the association occurs naturally when plants are transplanted into the field. The association of AMF with plant-parasitic nematodes and the beneficial effect of the mycorrhizal symbiosis on plant growth and nematode resistance/tolerance led to investigations into the potential of AMF to limit yield losses due to nematodes.In the first part of our study, the relative mycorrhizal dependency (RMD) and the AMF-nematode interaction were studied in four Musa genotypes, selected for their known host plant response to nematodes (i.e. 'Grande naine', 'Gros Michel', 'Pisang jari buaya' and 'Yangambi km5'). Mycorrhization with Glomus mosseae (AMF) resulted in a significant better plant growth, even in the presence of Radopholus similis and Pratylenchus coffeae. No differences in RMD were observed among the four genotypes. Glomus mosseae could protect 'Grande naine' and 'Pisang jari buaya' against R. similis and P. coffeae, since the nematode reproduction was suppressed. Only in the case of R. similis (Indonesian population with low pathogenicity) in 'Pisang jari buaya', no suppression was observed. However, when reproduction is already very low (due to low reproductive fitness of the nematode population and/or the resistant host plant response of the tested genotype), the presence of the AMF has no effect on the nematode reproduction. The AMF reduced the root necrosis, caused by P. coffeae. For R. similis, no reduction was observed. The nematodes reduced the frequency of mycorrhization, without reducing the intensity of the mycorrhizal association.In the second part, the RMD and the AMF-nematode interaction were studied in Musa genotypes differing in root morphology. The influence of the AMF on the root system and the influence of the altered root system on the nematode reproduction were examined. Mycorrhization with G. mosseae resulted in a significant better plant growth, even in the presence of P. coffeae. The effect of AMF on the root system was related to the RMD of the genotype. Musa genotypes with a low RMD will not experience changes in the branching of their root system in response to mycorrhization. But in genotypes with a high RMD, the root system will be more branched. We showed that P. coffeae also affects the root system, by reducing the branching. The effect of AMF on the nematode reproduction was not very clear. The nematode population density tended to be reduced, but was not significant in the experiment with 'Obino l'ewai'. In the root system, it appeared that the decreased branching caused by the nematodes was counterbalanced by the increased branching caused by the AMF. Therefore application of AMF could be used as a strategy to decrease susceptibility to nematodes.In the third part of our study, AMFnematode interactions were studied under in vitro conditions. Firstly, aseptic nematode cultures were established using alfalfa callus as a host tissue. Until now the lack of completely sterile culture systems limited in vitro nematode-host studies and AMF-nematode interaction studies. Secondly, three model systems were developed: Ri T-DNA transformed Daucus carota roots, in vitro Musa plants and in vitro Arabidopsis thaliana plants.Finally, the transformed D. carota roots were used to study the AMF-nematode interaction under sterile conditions. The results reported in this study confirmed the suppressive effect of AMF on nematode reproduction. Glomus intraradices could suppress the R. similis, P. coffeae and to a lesser extent M. javanica population in the roots. The internal and external developments of the AMF were not affected by the presence of these plant-parasitic nematodes.Although this in vitro system has several limitations, there are still many legitimate reasons to use this system to study the AMF-nematode interaction. The AMF develops appressoria, arbuscules and vesicules in the root cortex, produces profuse extraradical mycelium and spores and is completing its life cycle in vitro. The early colonization occurs in a similar way as under in vivo conditions. The nematodes, R. similis and P. coffeae, can infect and reproduce in the roots, and cause similar damage in the in vitro roots as in in vivo roots. In addition, the effects of the interaction reflect those observed in vivo. Although the dixenic system used is artificial, it may represent a valuable tool for studying the AMF-nematode interaction, complementary to classical experimental approaches. ■ The project will be implemented in two phases. The first phase includes the multiplication of planting material of at least ten improved Musa varieties in each country and the distribution of these plants to farmers for on-farm evaluation. At least 150 farmers per country participate in the trials. The second phase will consist of funding support in the form of loans to small-scale farmers to enable them to purchase planting material and essential inputs for the more wide scale production of improved hybrids. The project will also include market studies on the improved hybrids and the training of farmers in improved production techniques, focusing on the integrated management of pests and diseases. The main result from the project will be the increased production of improved Musa hybrids by small-scale farmers. These varieties will produce higher yields and will not require chemicals for pest and disease control. In addition, farmers and entrepreneurs will be assisted to set up banana-related businesses (the production of planting material for sale etc.), thus contributing to increased income generation for rural communities. The major beneficiaries will be small-scale banana farmers.For more information on the project, please contact Suzanne Sharrock, project coordinator, at INIBAP Headquarters.The 3rd International bacterial wilt symposium was attended by 110 scientists from all over the world who presented more than 100 papers either orally or as posters. Aspects discussed were: epidemiology, disease management, breeding and deployment for disease resistance, host response and disease development, pathogen genetics, diversity and diagnosis.Bacterial wilt caused by Ralstonia solanacearum is reported to be one of the major constraints for many important crops such as potato, tomato, groundnut, banana, tobacco and ginger. In many cases, the disease causes very significant yield losses.There is still a big gap in the research progress between developed and developing countries. In developed countries, scientists are generally more interested in the molecular aspects of the pathogen such as pathogen genetics, diversity and diagnosis. Except for diagnostics, other aspects under study are not directly related to control of the disease. Therefore, research being carried out on disease control by scientists in developing countries, where the disease is more serious and widespread, needs to be strengthened. Some work on breeding and deployment for disease resistance has been carried out and good results have been obtained for groundnut and potato. However, very little has so far been done for many other important crops such as banana and ginger.Significant progress has been made in studies of the genome of R. solanacearum. The pathogen has a 3,716,413 base pair (bp) chromosome and a 2,094,509 bp megaplasmid, which taken together encode over 5000 proteins. The chromosome harbours all essential genes, whereas the megaplasmid is involved in the biosynthesis of various amino acids, cofactors, and fitness to environments. There are about 200 candidate genes for pathogenicity distributed both in the chromosome and megaplasmid. This information is essential in understanding the biodiversity in R. solanacearum in relation to host specificity. Traditionally, R. solanacearum strains are grouped into five races, based on host range, and five biovars based on the oxidation on certain carbon sources.A new classification scheme has been proposed based on the molecular analyses of R. solanacearum. Strains of R. solanacearum are classified into four phylotypes such as Phylotype I 'Asia' (include biovars 3 and 4, race 1, 4 and 5), phylotype II 'America' (biovar 1 and 2, race 1, 2,and 3), phylotype III 'Africa' (biovar 1, 2), and phylotype IV 'Indonesia' (biovar 1, 2, Pseudomonas syzygii, and blood disease bacterium -BDB). This shows that strains of Indonesia, including P. syzygii which causes Sumatra disease of clove, and the BDB on banana, are separated from other strains.Various diagnostic kits have been developed, especially for quarantine purposes and for monitoring the pathogen in symptomatic and latently infected plant materials, surface water, soil, vegetable washings, and processing waste. Methods used are selective isolation and enrichment, bioassay, immunofluorescence, serology, and polymerase chain reaction (PCR). For use in developing countries, serology methods such as ELISA are more appropriate as they are cheaper.The 4 th Symposium will be held in 2007, possibly in the UK.The American Plant Pathology Society will publish the papers presented in the Symposium.Further information about the symposium is available from Dr Supriadi, Research Institute for Spice and Medicinal Crops, Jalan Tentara Pelajar No. 3, Bogor 16111, Indonesia. Fax: (0251) 327010.A preliminary report on the status of Musa acuminata in Northern Borneo has recently been prepared by Markku Häkkinen and Edmond De Langhe. This report is based on a survey of Musa in Sabah, Sarawak and Brunei carried out by the first author in August 2001. Although the main focus of the survey was the section Callimusa, a large number of photographs of Musa acuminata were also taken. With the expertise of Edmond de Langhe, a tentative taxonomic identification of the plants has been made.The photographs showed that all the plants exhibited the basic characteristics of M. acuminata, with the typical top-like male bud, the horizontal-to-oblique inflorescence and bunch, and rather slender fingers. The flowers appeared to be white-to-creamy, but the details were not visible in the photos.The specimens in question were found to fall into four main categories: • M. acuminata ssp. microcarpa or truncata • M. acuminata of uncertain status • Edible AA diploids • Unclassified accessions.The most important result of the Häkkinen visit to Northern Borneo, an area previously little explored for wild bananas, is the domination of a wild M. acuminata population with a combination of characteristics typical of the subspecies microcarpa and truncata. The occurrence of yellow-green male bud types is the first time this character has been recorded for these subspecies. It is possible that the two supposed subspecies may actually form one large and compound population, and further studies on this material in a field genebank are required to confirm the status of the accessions in this group.A number of accessions were characterized by a moderately to strongly imbricated male bud and a trend for the bract colour to be pink/red/purple. Male buds with visibly imbricate bracts are characteristic for the subspecies siamea and burmannica, but are not expected for the acuminata's in Borneo or the Indonesian islands, with the exception of Java. Further studies are required to confirm if these are edible diploids or indeed truly wild plants.A number of plants are recorded as edible AA diploids. Amongst these are plants that were found to be populating large areas along the sides of roads, as truly wild populations do. Since there were no villages in the proximity, these may be the remnants of human population from remote time.The findings presented in this report are clearly of a preliminary and tentative nature, as they are based on studies of photographs only. However, it does provide a basis for further studies, which the authors hope will be stimulated by this report.Copies of the report, including colour photographs of all the accessions, are available in PDF format from the INIBAP web site (http://www.inibap.org/publications/borneo.pdf) or in printed form from INIBAP Headquarters, Montpellier.Inge Van The general infrastructure of the laboratories was improved and various items of equipment were bought. An e-mail/internet connection was established in order to improve the communication between the different project partners and with other nematologists in the world, and to have access to information from the worldwide web.During the time of the project, two staff members of the Agrobiotechnology Department of VASI followed the postgraduate international nematology course in Belgium. In this respect:Duong Thi Minh Nguyet defended her MSc thesis entitled: «In vivo and in vitro studies of Radopholus similis and Pratylenchus coffeae associated with banana» in 1999 (Promotor: Prof. D. De Waele).Nguyen Thi Tuyet defended her MSc thesis entitled: « In vitro and in vivo screening for Radopholus similis resistance in Musa» in 2000 (Promotor: Prof. D. De Waele).species in natural habitats in North Vietnam Three survey trips were undertaken in natural habitats in North Vietnam and root samples from three species of wild bananas were taken. With the exception of R. similis, the most important Musa nematode species, i.e. Meloidogyne spp., P. coffeae and Helicotylenchus multicinctus, were found. This indicates that the natural soils in Vietnam are infested with these nematode species and that the three wild banana species are susceptible to these species.Five survey trips were undertaken in six provinces in North and Central Vietnam and root samples of three commonly cultivated banana genotypes were taken. Again, Meloidogyne spp., P. coffeae and H. multicinctus were found, but not R. similis. Damage parameters showed a clear relation with the presence of certain nematode species in the roots. For example, root-knot galling was positively correlated with the number of Meloidogyne spp. in the roots, while root necrosis was positively correlated with the number of P. coffeae found in the roots.population and Meloidogyne spp. on plant growth and yield of banana A field was planted with over 150 banana plants, of which one third was inoculated with P. coffeae, one third with Meloidogyne spp. and one third was kept nematode-free (control plants). The preliminary results showed that infection with P. coffeae and Meloidogyne spp. can reduce the plant height and the number of standing leaves in comparison with the uninfected control plants.The reproduction of P. coffeae on carrot discs under in vitro conditions could be described by the Gompertz equation: log (nem + 1) = 0.725 + 2.561 exp [-exp (1.742 (5.044 -time))].From a greenhouse experiment that was repeated monthly over a period of one year, it could be seen that temperature has a strong effect on the reproduction rate of P. coffeae on bananas. During the winter months, the reproduction was very low, while during the summer months, the population increased significantly. The extent of root-necrosis followed more or less the same pattern.A greenhouse experiment was set up to assess the influence of irrigation on the reproduction of P. coffeae. A shortage of water had a very strong negative effect on the plant growth, while the nematodes could still reproduce well. A very high application of water reduced the general plant growth slightly, but the nematodes could barely reproduce. An intermediate water volume was best for the growth of the plants, but also favourable for the nematode reproduction.The reproduction of a P. coffeae population on banana plants in the field was followed for more than one year. From preliminary results, it can be seen that temperature and rainfall have an effect on the reproduction rate of the nematodes.germplasm for resistance to a Pratylenchus coffeae population in the greenhouse Twenty-four Vietnamese banana genotypes were screened for resistance to P. coffeae in the greenhouse. The most promising genotypes are 'Tieu xanh', 'Tieu mien nam', 'Com chua', 'Com lua', 'Man' and 'Ngu thoc'.germplasm for resistance to Meloidogyne spp. in the greenhouse Twenty-two Vietnamese banana genotypes were screened for resistance to Meloidogyne spp. in the greenhouse. No sources of resistance were found.germplasm for resistance to Meloidogyne spp. in the field Eight Vietnamese banana genotypes, 'FHIA-01', 'FHIA-02' and 'Yangambi km 5' were evaluated for their host plant response to Meloidogyne spp. under field conditions. 'FHIA-01', 'Ngu thoc', 'Tay' and 'Com lua' were found to be less susceptible to Meloidogyne spp. 'FHIA-01', 'Ben tre' and 'Bom' were less sensitive to the knot-forming activity of Meloidogyne spp. The number of juveniles recovered from the roots was strongly influenced by the weather. During the cold and dry season, the numbers dropped very significantly. The number of egg-laying females in the roots (ELF) was much less influenced by the environmental conditions: there was a stagnation during the cold and dry season but no decline. Meloidogyne spp. seem to overwinter as eggs in egg-masses. Root-knot galling and ELF can be used as easy parameters to estimate the infection of Musa with Meloidogyne spp. No effects of the nematodes on plant growth were found. The number of nematodes in the roots seems to be related to the physiological stage of the plants. The highest nematode numbers were found during flowering.Duong Thi Minh Nguyet started a research programme on the occurrence of Radopholus similis in Vietnam and its morphological and biological aspects. Two surveys were carried out in Tay Nguyen (Western Highlands) to assess the occurrence of R. similis on coffee, durian, bananas, etc. One R. similis population was collected from durian roots and is being maintained on carrot discs under in vitro conditions. Since R. similis is still a quarantine pathogen in Vietnam, Duong Thi Minh Nguyet went to Belgium for a period of three months to study the collected population. She determined that the optimal temperature for reproduction of R. similis from Vietnam on carrot discs was 25°C. She also compared the reproduction of the Vietnamese population with populations from Indonesia and Uganda.Nguyen Thi Tuyet is studying the biodiversity of Pratylenchus coffeae in Vietnam. She is collecting P. coffeae populations from various crops and places in Vietnam to study the morphological, biological and genetic diversity between the different populations.Banana wilt caused by Fusarium oxysporum f. sp. cubense is one of the serious threats to banana cultivation in Kerala. The acidic soils of the state and the susceptibility of the major commercial varieties offer an easy spread of the disease throughout the region, causing a yield loss of 10-15%.The symptoms of the disease appear with the yellowing of older leaves which extends rapidly from the margin towards the midrib. These leaves hang withered around the pseudostem and the infection spreads to all leaves except the top, which hang down. The heartleaf also withers after 3-4 weeks. The plant exhibits longitudinal splitting with bulging and elongation of the pseudostem. When the rhizome is cut open, the discolouration of the vascular bundles can be seen and the cut stem smells of rotten fish.A survey conducted by Estelitta et al. revealed that the disease was prevalent in all the districts in Kerala causing serious damage to the crop. It was also noticed that 'Nendran', the most important commercial variety in the State was susceptible to Panama wilt while Cavendish group, 'Palayankodan', 'Karpooravally' and cooking varieties such as 'Monthan', 'Kanchikela', etc. were not seen to be affected by the disease.Several studies were conducted at the Banana Research Station, Kannara under Kerala Agricultural University on integrated management practices for Panama wilt disease. The pathogen was found to be soil borne and its entry to the host plant was through roots. Since the conidia can survive in the soil for as long as seven years, a package of agronomic practices are recommended based on the findings of the studies.Field preparation should be carried out in a systematic way. In disease affected locations, weathering the pits for a week or more and burning the soil with dry leaves is recommended. Field sanitation, especially removal of grass weeds is necessary as they become critical alternate hosts.Cultivation of tolerant varieties is suggested for disease prone areas. In other cases, suckers should be selected from disease free areas. Dipping pared suckers in 0.2% solution of carbendazim was also found to be an effective prophylactic measure.It was also found that the application of lime at 1 kg per plant as a soil amendment at the onset of the monsoon and good drainage were helpful in checking the disease.The studies further indicated that use of organic manures in banana cultivation could give a better stand of the crop against the disease, probably due to improved soil structure with more aeration.In the case of disease occurrence, removal and destruction of the diseased plants is recommended to check its further spread. Application of lime at 0.5-1 kg in the diseased plant pits and in the basins of surrounding plants also gave encouraging results in checking the further spread of the pathogen.From experiments carried out on different chemicals to control Panama wilt, it was found that corm injections with 2% solution of carbendazim at 3ml/corm during the 5 th , 7 th and 9 th month after planting could help to control the disease. Drenching the soil with 0.2% carbendazim was also found to be effective.Since the commercial banana varieties in Kerala are often cultivated in extensive wetlands, studies indicated that crop rotation, intermittent fallowing or flooding followed by fallowing are also effective ways of reducing the spread of the disease.Further information is available from S. Estelitta, Associate Professor, Kerala Agricultural University, Mannuthy, Thrissur, Kerala, S. India.Before initiating a hybridization programme in banana breeding, cross-compatibility between desirable parents has to be assessed. Such work is presently ongoing at Tamil Nadu Agricultural University (TNAU) in India. Seventeen varieties, including commercial triploids, diploids and TNAU-bred synthetic hybrids were included in the study (see Table 1). Anthers were collected from the male parents just prior to dehiscence and the pollen grains were extracted and smeared on the stigma of the female flowers of female parents on the day of opening in the early morning between 6.00 to 9.00 a.m., when the receptivity of stigma was good and ensured by stickiness by touch. After pollination, the flowers were covered with perforated paper bags. Once ripe, the fingers were longitudinally cut and the seeds, if present, were extracted carefully.Among the 74 cross combinations tested, compatibility was found only in eight combinations (Table 2), thus indicating the existence of compatibility between clones. The successful crosses were between diploid x diploid and triploid x diploid. Out of the 10 male parents tested, 'Pisang lilin' and 'Anaikomban' were compatible with all female parents. 'Nendran' which was earlier reported as female sterile (Alexander 1970) was confirmed as female sterile in this investigation. The study indicated that with the exception of 'Karpooravalli', the clones of commercial importance have a very low percentage of female fertility, and diploids provide the best female fertile clones. Seed set was however good in the triploid x triploid cross of Karpooravalli x Robusta, which indicates the possibility of new line in banana breeding, bringing the 'Cavendish' genome into new hybrids. H-201 (Pedigree: Bareli chinia x Pisang lilin x Robusta) is a good female parent and has hybridized with diploid as well as triploid parents (viz. Robusta). Sathiamoorthy and Balamohan (1993) reported that H-201 was a potential female parent par-ticularly in the synthesis of triploids of bispecific origin. Seed production was maximum in H-201 x Pisang lilin followed by H-201 x Anaikomban and Karpooravalli x Robusta. In other successful combinations, seed production was very low despite the crosses being compatible.More information about this work is available from: V. Krishnamoorthy, Dept of Fruit Crops, Horticultural College and Research Institute, TNAU, Coimbatore-641003, Tamil Nadu, India. The northeastern states of India, namely Assam, Arunachal Pradesh, Megalaya, Tripura, Mizoram and Manipur are a rich source of natural diversity in Musa. Since 1998, INIBAP has been supporting a series of Musa collecting missions in this region. These have been conducted by the National Research Centre for Banana (NRCB), Trichy. Specimens of the wild species Ensete glaucum, Musa balbisiana, M. acuminata, M. ornata and other Rhodochlamys species have been collected, together with a range of cultivated varieties. All the collected material has been established in the NRCB genebank for formal identification and characterization.In Arunchal Pradesh, it was noted that where Eumusa and Rhodochlamys bananas grew together, the Rhodochlamys had a closer eco-compatibility with M. acuminata than M. balbisiana, although M. acuminata was clearly dominant over the Rhodochlamys species. In Assam, Bhimkol, a seeded balbisiana clone is widely grown in backyards for its medicinal qualities. Although seeded, the seeds are soft enough that the fruit can be consumed along with the seeds. Throughout the region, the unusual practice of selling male buds was noticed. The immature male buds of wild bananas are harvested even before shooting and are used for the preparation of special dishes. A summary of the wild species collected during the missions is provided in Table 3.Hélène Laurence, an intern funded by the Ministère des Relations internationales du Québec joined INIBAP as a Research Assistant in January 2002. She is based at the Regional Office of INIBAP in Kampala and will begin work on a 3-year study of the impact of improved banana varieties (Musa) on the livelihoods of households in Eastern Africa. She will work closely with INIBAP scientists and regional NARS partners. Hélène has a BSc in geography and a MSc in Agrometeorology.Olivier Guinard, also funded by the Ministère des Relations internationales du Québec, joined the INIBAP programme in Montpellier as an intern in April 2002. During his 6-month internship he will be working on a project using the MGIS database (Musa Germplasm Information System) to carry out a series of tracer studies on important germplasm accessions as well as visualizing geographical assignments for the different accessions. Olivier comes from Québec where he studied for his BSc in Biology, specializing in molecular biology/biotechnology at the University of Quebec in Montréal and has just completed his MSc.A training course on the use of the Musa Germplasm Information System (MGIS) for the management of information related to genetic resources of bananas and plantains (Musa spp.) was held recently for Musa germplasm curators from Africa. The objective of this training course was to provide these curators with the expertise and tools to better manage information related to the accession in their collections. The use of MGIS will also allow them to exchange genetic resource information with other researchers and curators throughout the world. This training course was held thanks to funding support provided by the Technical Centre for Agricultural and Rural Cooperation (CTA).The training course gathered 23 participants from West, Central, Eastern and Southern Africa (see list below). The course was held in French and English, with translation ensured by the trainers. All documents and training materials were provided in both languages.The course included both field and computer-based training. Exercises on the taxonomical and botanical identification of varieties were held in the field using the list of descriptors published by IPGRI, INIBAP and CIRAD. The large germplasm collection maintained by CARBAP provided an excellent resource for the field exercises. This collection consists of over 400 accessions, representing a very large range of African varieties, especially plantains, but also including some East African highland varieties.With regard to the computer-based training, participants learnt how to install the MGIS software on their computers, how to create users accounts, enter new records, and carry out information searches in the global database. They were also trained in the procedures for data exchange through the global database.Musaid.win, a software developed by CIRAD to assist in the identification of unknown varieties, was also explained by Xavier Perrier from the Biometry Unit of CIRAD. This software is provided to all participants in MGIS.Participants agreed that the training provided a useful tool for the management of genetic resource information and highlighted the importance of collecting and managing this information using a standard format. The workshop also provided a valuable opportunity for curators to make contact with their colleagues from the region as a whole, and also to identify resource people who can help them in their future work.Following the MGIS training course, a workshop on the \"Names and synonyms of plantains\" was held with the participants from West and Central Africa. More information about the DIVA software is available from the CIP website (http://www.cipotato.org/diva/). The software is freely available and can be downloaded, along with the user's manual, from the website.Fifth meeting of the regional steering committee of MUSACO From 11 to 12 February 2002, the regional steering committee of the banana and plantain research network for west and central Africa, MUSACO, met in Cotonou, Benin for its annual meeting. In attendance were representatives from Benin, Cameroon, Congo Republic, Côte d'Ivoire, Gabon, the Democratic Republic of Congo, Ghana, Guinea, and Sierra Leone as were those of CARBAP (formerly CRBP) IITA and INIBAP. Togo was admitted as the 13 th member of the network at this meeting.In a speech to officially open the meeting, Dr David Arodokoun, Scientific Director of the Institut national des recherches agricoles du Bénin acting on behalf of his Director General stressed that banana and plantain are crops that could contribute to food and nutritional security, alleviation of poverty, and the creation of employment in Benin. Banana and plantain are two of the crops that Benin is now promoting to diversify the food crop base. He reported that 33% of households in Benin regularly consume plantains and Musa production in Benin has increased from 22 000 tonnes in 1998 to 45 000 in 2001. He hoped that the research carried out in the network will help find solutions to constraints facing farmers in Benin such as lack of clean planting materials, pests and diseases and post-harvest transformation of the fruit.The first order of business was the adoption of the minutes of the last regional steering committee meeting held in Accra in April 2001. Before members would do that, they wanted to know if the recommendations of that meeting had been implemented. The President and secretary provided the following information on the various recommendations that were made in Accra: Each country representative gave a brief report on new activities that are taking place in their countries. To popularize banana and plantain production in Sierra Leone, nurseries and demonstration plots have been established in four districts. These will be extended to four more districts. Also, local varieties have been collected and planted for characterization purposes.As in Sierra Leone, to re-launch plantain and banana production in Littoral and in the Forest regions of Guinea (Conakry), nurseries have been established to produce clean planting materials that will be distributed to farmers. Among the varieties to be given to farmers are hybrids of IITA and CARBAP Studies are being planned in Côte d'Ivoire to attempt to explain the observation that Pratylenchus spp. seems to be replacing Radopholus similis as the main nematode on Musa. IITA is interested in the issue of changes in species composition and will consider offering a doctoral fellowship for work on this in collaboration with KULeuven. It was recommended that a nematological survey be conducted in all member countries to determine if the nematode diversity and the relative abundance of the species remain the same as before.The other new activity in Côte d'Ivoire was the high planting density technology that Ivorian scientists are testing on-station. The high planting density trials being conducted in Côte d'Ivoire and also in Cameroon are the follow-up of a visit made by ten scientists, farmers and extension agents from West and Central Africa to the Dominican Republic and Costa Rica to study the high density plantain plantation production technologies being used there. The delegation was impressed by the up to 60% yield increases that have been obtained in the Latin America and Caribbean region from managing 'False horn' plantain as an annual crop at densities from 2500 to 5000 plants per hectare.Ghana's new representative on the steering committee, Dr Anno-Nyako informed the meeting that Musa breeding has started in Ghana. A plant biology laboratory with a tissue culture unit has been established in Gabon.Being the first time to participate in the meeting, the Togolese representative, Dr S. Dogbe, gave an overview of the research on banana and plantain in his country. Bananas and plantains are cultivated in the cocoa/coffee zone as a shade for the cash crops. The programme has a field genebank consisting of 32 accessions many of which originate from Ghana. A major problem confronting the production of the crops in Togo is the lack of good quality planting materials.Members reported on the two regional projects, Musa germplasm evaluation and periurban Musa production.Germplasm evaluation trials have been established in almost all the network countries but it is only in Côte d'Ivoire that the first crop has been harvested. It was reported that 'FHIA-23' is the most productive among the bananas ('FHIA-01', 'FHIA-18' and 'SH-3460') that are being evaluated in Côte d'Ivoire, but it also has the longest crop cycle. The inoculum pressure of black leaf streak disease (BLSD) was so high that at harvest, 'Orishele', the susceptible plantain variety had virtually no functional leaves. Consequently, bunch weights of 'Orishele' were low compared to the resistant hybrid, 'CRBP-39', which maintained six green leaves to harvest. Tests to determine consumer acceptance of the fruits are being conducted but the results are not yet available.The periurban Musa production project is making good progress in Benin with the vitroplantlets provided by CARBAP. Among the hybrids established in Benin are . Survival of plants in the field was very high so is the enthusiasm of the 40 farmer participants. The visit to some of the farms generated a lot of interest as the plants were doing very well. The researchers and farmers exchanged views on farmer participatory research approaches. These discussions continued when the scientists returned to the meeting place the next day. A survey on periurban Musa production conducted in Benin revealed that 56% of Musa farmers cultivate plantain on an average plot size of 0.8 ha. In Benin also, inadequate supply of planting materials is cited a bottleneck to expanded productionThe periurban project has not really taken off in Ghana as the national institution that was supposed to supply the planting materials failed to do so. Materials imported from South Africa have been weaned and will be supplied to farmers at the beginning of the rainy season.CARBAP and IITA each sent a delegation of several scientists to present the different areas of Musa research at their centres. Dr Lutaladio representing FAO talked about collaboration with the network.CARBAP presented the advances of their programmes regarding plantain breeding, agronomy and integrated systems, plant pathology and pest control with an integrated pest management approach (leaf spot diseases, nematodes and weevil), post-harvest technologies and socio-economics. Achievements of the centre include the development and transfer of in vivo multiplication techniques to produce large numbers of clean planting materials, and 'CRBP-39', a plantain-like hybrid that has been released and distributed within the MUSACO network and to more than 40 countries around the world in the framework of the 3 rd International Musa Testing Programme coordinated by INIBAP. Secondary triploids with resistance to diseases have been created by different breeding schemes and short and early yielding plantain hybrids are also under evaluation. Banana and plantain-based snack foods, infant formulas and flour have been developed.Presentations from IITA were on participatory research, integrated pest management, Musa breeding and agronomy. In farmer participatory evaluations conducted in eastern Nigeria, farmers preferred 'PITA-14' one of IITA's plantaintype hybrids over 'Agbagba', the local variety. 'PITA-14' gave higher financial returns as well. The meeting was informed of a pilot project supported by USAID in which hybrids developed at CARBAP, IITA and FHIA are being evaluated in farmers' fields in Nigeria. It is hoped that after the pilot phase, the project will be expanded to other countries in the West and Central Africa. IITA continues to develop plantains hybrids with superior resistance or tolerance to diseases, including a practical physiologicalgenetic and biotechnological approach to control banana streak virus, and good agronomic characteristics such as earliness, short stature, and good rooting. Integrated pest management research has included developing and testing methods such as using hot or boiling water to clean suckers contaminated with pests. There are also studies to determine the efficacy of nematicidal plants such as Flemingia interplanted in plantain fields.The four projects of INIBAP, namely germplasm management, germplasm improvement, information and communications and regional networks were briefly described and activities in Africa under each were mentioned. The objectives and modus operandi of PROMUSA, the global programme for Musa improvement, coordinated from INIBAP headquarters were also described.The FAO representative at the meeting, Dr Lutaladio, traced the history of the collaboration between his department and INIBAP. In 1999 AGPC/FAO and INI-BAP discussed collaboration on the gathering and exchange of information and the transfer of technology. A young professional officer (YPO) was recruited and posted to the INIBAP/MUSACO secretariat to develop instruments for the collection and compilation of baseline information and to incorporate collected information into HORTIVAR, a database on performances of horticulture cultivars in relation to environmental conditions and cultivation practices. In addition, the YPO was to provide inputs in the urban and periurban horticulture programme in relation to food security and was to assist in project proposal development. Dr Lutaladio informed the meeting that the YPO has prepared preliminary reports on the different tasks he was given at the beginning of his assignment.In the immediate future the FAO will assist at least two MUSACO member countries in designing projects for improvement of banana and plantain production for small-scale growers through the setting up of efficient and cost-effective multiplication systems for production of disease-free planting materials. The FAO will collaborate with MUSACO, CARBAP and IITA to collect and characterize Musa germplasm in the Congo basin, to upgrade tissue culture and nursery facilities in certain countries, and to train researchers in handling tissue culture plantlets, virus indexing, and rapid production of planting materials. Finally, the FAO will assist in the development of a protocol for mass propagation and distribution of quality planting materials.The delegates discussed ways to improve the operation of the network. For a start, it was agreed that working groups based on identified research priorities should be set up. These will meet as often as necessary depending on available resources. Working groups are to be formed immediately on (1) rapid multiplication of clean planting materials; (2) profitable plantain production systems and ( 3) farmer participatory research. The secretary of the network was asked to identify leaders for each of the three working groups. Activities proposed by the working groups will be approved and their implementation monitored by the regional steering committee. The current unsatisfactory communication links among members and between members and the secretariat was attributed to the lack of information technology equipment in many of the research stations where the Musa programmes are based.The assembly passed the following recommendations: Coming 13 years after the last international meeting on this topic, it provided a timely opportunity to analyse the current situation regarding Mycosphaerella leaf spot diseases at the global level. The meeting also allowed new lines of investigation to be suggested and facilitated the re-orientation of breeding programmes and biotechnology strategies for the genetic improvement of bananas and plantains.More than 60 scientists attended the workshop from both the private and public sectors, representing more than 16 different countries from Latin America and the Caribbean, Europe, Africa, Asia and the Pacific, including Australia.In order to maximize the outputs of the meeting and to guarantee the development of new strategies in the control of the different Mycosphaerella leaf spot diseases, participation in this workshop was by invitation only. The results of the meeting will however be widely disseminated through the publication of the proceedings.The meeting was inaugurated by Dr Jorge Sauma, Director of CORBANA. Dr Emile Frison, Director of INIBAP, welcomed all the participants and paid tribute to Ramiro Jaramillo Celis, former INIBAP regional coordinator for Latin America and the Caribbean, in recognition of his invaluable contribution and tireless efforts to the development of the regional Musa research network. The official opening was made by Dr Salvador Monge, Executive Director of the Secretary of sector-based planning of the Ministry of Agriculture of Costa Rica.The workshop was organized around five main topics: 1) Impact of Mycosphaerella leaf spot diseases of bananas; 2) Population biology and epidemiology; 3) Host-pathogen interactions; 4) Genetic improvement for a management of durable resistance and 5) Integrated disease management.During the workshop, participants had the opportunity to learn about the distribution and impact of the different Mycosphaerella leaf spot diseases in several countries around the world. Discussions were held at the end of the each session to allow research priorities and corresponding activities required at the global level to be identified or refined, in order to significantly reduce the impact of these diseases and thus make Musa a more sustainably productive crop.Introductory papers presented information on the global spread, current distribution and impact of the three Mycosphaerella leaf spot pathogens -M. musicola, M. fijiensis and M. eumusae. Other papers described techniques developed in Australia for the rapid diagnosis of M. musicola and M. fijiensis, the effects of M. musicola and M. eumusae on banana cultivation in South Africa and the impact of M. fijiensis in Cuba, Brazil and tropical Asia. The latest taxonomic work undertaken on the anamorph of M. eumusae and on other Mycosphaerella species was also described. M. fijiensis continues to spread to new areas. From 2000 to 2002, the pathogen was identified for the first time in Madagascar, the Bahamas, and the Galapagos Islands of Ecuador and in the north Queensland banana growing area where eradication is being attempted. M. eumusae leaf spot has also been observed on 'Mysore' (AAB) in Sri Lanka. As this clone has strong resistance to M. musicola and M. fijiensis, this is the cause of some concern.It was agreed that more taxonomic information about Mycosphaerella spp. is needed, as well as information on other related genera that either form or occur in banana leaf lesions. A greater knowledge of Mycosphaerella pathogens /saprophytes and those in related genera is a prerequisite to the development of rapid diagnostic tests to distinguish leaf spot pathogens. The exact distribution of M. eumusae also needs to be investi-gated. Further surveys in South and Southeast Asia to determine where M. musicola, M. fijiensis and M. eumusae occur are necessary. More information on the effect of M. eumusae on the growth and yield of banana clones is needed. Information suggests that Cavendish and Plantain cultivars are very susceptible.Pathogenicity and distribution variability, sources of resistance, epidemiology and population structure of the main species (M. fijiensis, M. musicola and M. eumusae) at the national, regional and international levels were defined as fundamental information for the continued success of banana production. Such studies are particularly necessary in Asia, which is the centre of diversity of the three pathogens and where little research has so far been conducted. A study of the evolution of host-pathogen relationships for the three pathogens, particularly involving resistant cultivars, is of a special concern, in order to identify pathogen populations that could break down plant resistance and to evaluate selection pressure. Molecular tools such as microsatellites have been recommended to monitor the genetic variability of the pathogen populations and pathogenicity should be evaluated concurrently. Epidemiological studies, including disease dispersal, are needed to better understand the distribution and the spread of the pathogen and will complement, together with the studies on pathogenicity and genetic variability, all the information required to anticipate the evolution of pathogen populations and to define resistance management strategies.Several cases of an unexpected level of susceptibility to black leaf streak disease (BLSD) have been reported. Although different reasons have been offered to explain the phenomenon (poor nutrition, environmental stress), the problem of the erosion of resistance cannot be ignored and requires a precise characterization of the pathogen population. A greater understanding of the mechanisms involved in plant-pathogen interactions continues to be needed to ensure the long term success of breeding programmes. Further studies are also needed to compare the effect of infection by each of the three pathogens (M. fijiensis, M. musicola and M. eumusae) on the host plants.Other pathosystems (such as Magnaporthe grisea) have shown the powerful nature of the genetic approach to identify without any a priori the pathogenicity factors. These approaches include the study of gene expression during production of pathogenicity mutants, comparative genomic and gene function validation techniques. Consequently, the development of genetic and molecular biology tools for M. fijiensis in collaboration with M. graminicola groups and the launching of a genomic initiative to access genomic tools and set up a genomic-wide comparison of M. fijiensis with M. graminicola have been recommended.It is also recommended that the different mechanisms of resistance (partial or vertical) should be studied.During this session, progress that has been made towards the creation of new varieties resistant to BLSD, either through conventional and/or modern technologies, was presented. New tetraploids hybrids resistant to BLSD are already available and some of these are widely grown around the world. However, because of the lack of new sources of resistance and due to the presence of the activable form of the banana streak virus (BSV) in interspecific hybrids (A x B), the production of a new generation of triploid hybrids is seriously jeopardized. Good progress has been reported in the development of a molecular toolbox for bananas and plantains in the area of the genetic transformation, allowing the production of transgenic banana plants.The study of the diversity of the Musa balbisiana genome, using both morphological and molecular characterization was the first recommendation from this session. It was also recommended, that in anticipation of the needs of genetic improvement programmes, the T and S genomes, Musa textilis and Musa schizocarpa respectively, should also be screened.Mutation induction techniques should no longer be seen as an independent genetic improvement strategy but more as a tool that can contribute to crossbreeding programmes by increasing genetic diversity of parental lines. Mutants could also help in understanding the mechanisms of resistance (functional genomics).Yellow and black Sigatoka control strategies on banana can, according to the country and the scale of production, include not only chemical and cultural practices but also the use of mixed crops or resistant clones. The important inhibitory effect of some natural substances derived from microorganisms antagonistic to fungi, have also been reported as effective in reducing the development of M. fijiensis in vitro.The integration of various or specialists from different disciplines has been recommended to facilitate the development of an achievable integrated pest management (IPM) approach for banana leaf spot diseases. The participants in the workshop also recommended to investigate the potential of natural or synthetic substances able to promote or activate systemic acquired resistance in its broadest sense.The proceedings of the workshop will be published shortly by INIBAP. This publication will include the full papers of all presentations and a summary of the discussions and recommendations. It is expected that the proceedings will become a reference document of Mycosphaerella diseases for the next ten years.Report of a meeting held in Arlington, USA, 17-20 July 2001 ISBN: 2-910810-48-08 The Global Musa Genomics Consortium was launched at a meeting held in Arlington, Virginia, which was critical for laying a solid foundation for future collaboration in Musa genomics and allowed the first important steps to be taken towards the development of a coherent strategy for Musa genomics.This document provides further background information about the establishment of the Consortium and its aims and objectives. It also provides a review of the current status of Musa genomics research and provides details of the nature and scale of the work to be carried out by the Consortium members. Information is provided on an incremental strategy developed by the Consortium to achieve its goals and the proposed modus operandi, as agreed during the Arlington meeting. Further details are provided in the Annexes.Copies are available from INIBAP Headquarters.To complete the revised version of the 'Descriptors for Banana, Musa spp.' and responding to demand from East Africa, additional characters specific to the East African Highland bananas were incorporated as an addendum in 2001. The descriptors for Musa are unique in including a colour chart. This helps to remove the subjective nature of colour recording, and leads to a common understanding of such characters.Copies of the descriptors and their addendum are available from INIBAP Headquarters.Edited by D.G. Cayón and F. Salazar Alonso ISBN: 958-96885-1-9 CORPOICA could certainly be proud for the effort they made recently to identify, analyse and compile the existing information on plantain research and technology transfer in Colombia under the title 'Resúmenes Analíticos de la Investigación sobre Plátano en Colombia'. This information product is a unique inventory of the major part of the scientific literature published on this topic including grey literature. It includes 792 abstracts and authors-and thematic indexes which make the search easier for the reader.This important document in Spanish is available in both electronic (database on CD-Rom) and printed (400 pages) formats and will certainly be highly appreciated by all those working on plantain at international level.The document is available on request at CORPOICA, Apartado Aéreo No 1087, Av Bolivar Sector Regivit 28 Norte, Armenia, Quindío, Colombia -Fax: ( 57 Information series Q101013 ISBN 0727-6273 During the period 1987-1996, the Queensland Department of Primary Industries (QDPI) was the lead agency of the ACIAR project 'Banana improvement in the South Pacific'. In the course of the project, banana varieties were collected from all around the world. One hundred and six varieties are reported in this publication which represent significant cross-section of those available for evaluation. They include some hybrids from conventional breeding programmes, selections originating as offtypes from tissue culture propagation, existing cultivars and wild species.The report put together the agronomic information collected along with colour photographs of bunches which permit a good appreciation of each variety. Many readers will also find these photographs useful for identification purposes.Clean & green bananas -Where to from here?Information series Q101014 ISBN 0727-6273 Current sales for clean & green/organic bananas are very limited in Australia. Organic export is risky and uncertain. However, the market is shifting in this direction and in the longer term, the organic niche will probably eventually grow to 10-15% of the market. Major sales of both products will be facilitated by supermarket participation and the current price of the existing organic product will need to come down. Efficiencies of production must be improved by specific research on limiting factors such as soil fertility management, leaf disease control and greater development/extension/adoption of existing technology. Good progress has been made by the banana industry to reduce pesticides use but it is now necessary to pull together the body of knowledge and develop an ECO-OK type system implemented on commercial farms which complies to auditable standards and market development so that producers are rewarded for their efforts.The two publications mentioned above are available on request at Department of Primary Industries, GPO Box 46, Brisbane QLD 2001, Australia. text, followed by the acronym in parenthesis.• References: All literature references made in the text should be referred to by author(s) and year of publication (e.g.: Sarah et al. 1992, Rowe 1995) : These should be numbered consecutively and referred to by these num-ber in the text. Each table should include a title. Illustrations: These should be numbered consecutively and referred to by these numbers in the text. Each illustration should include a clear and simple caption. Graphs: provide the corresponding raw data with the graphs. Drawings: provide originals if this is possible. Black and white photographs: provide them on bright paper and with good contrast. Colour photographs: provide good quality proofs and films or original slides. Note: When plant material used for the experiments reported originates or is registered in the INIBAP genebank, its accession number (ITC code) should be indicated within the text or in a tabular form.these instructions This will facilitate and accelerate the editing work.The meeting started with a brief presentation by each participant of their research capacity, in terms of human resources, research facilities, and future and ongoing projects related to Mycosphaerella leaf spot diseases. Participants also commented on their participation in PROMUSA, defining their areas of interest where they would like to develop partnerships with other participants. The participants identified various research priorities and defined the main activities that should be carried out.The survey of the distribution of the different species requires wide sampling at the national level of the different agroecological areas where Musa is found, and the morphological characterization of the species through the observation of the anamorph stage (conidia), including molecular characterization using PCR diagnostics.The PROMUSA Sigatoka working group ratified the recommendation made during the \"2 nd International workshop on Mycosphaerella leaf spot diseases of bananas\" held from 20 to 23 May 2002 in San Jose, Costa Rica: 'The exact distribution of M. eumusae needs to be known. Further surveys in south and southeast Asia to determine where M. musicola, M. fijiensis and M. eumusae occur are necessary. The name of the banana clone affected, an indicator of the severity of the leaf spot and local environmental data would be useful as this may help explain distribution. IMTP trials are seen as ideal locations for assessing the reaction of different clones to the different leaf spot pathogens. The collection and diagnosis of specimens of leaf spot from IMTP trials sites needs to be continued. The cooperation and collaboration of scientists in south and southeast Asia is viewed as essential. Identification tools should be provided to enable diagnoses to be undertaken locally '.The PROMUSA Sigatoka working group ratified the recommendation made during the \"2 nd International workshop on Mycosphaerella leaf spot diseases of bananas\": A reliable, rapid test to distinguish M. musicola, M. fijiensis, M. eumusae and possible other Mycosphaerella pathogens/saprophytes needs to be developed to aid identification. Information on how to distinguish the 3 pathogens on morphological characteristics also needs to be produced and circulated to banana scientists. INIBAP was asked to address this need\".The creation of a national collection of different strains from different Mycosphaerella leaf spot pathogens on Musa is of special relevance in the understanding of the population structure. The collection must be based on single-ascospore cultures with an in vitro characterization of the anamorph stage (in vitro sporulation of conidia). The Global Programme for Musa Improvement (PROMUSA) is a broad-based programme which aims at involving all the major players in Musa improvement. It was developed as a means to link the work carried out towards addressing the problems of export banana producers, with those initiatives directed towards improving banana and plantain production at the subsistence and smallholder level. The global programme builds upon existing achievements and is based upon ongoing research initiatives. PROMUSA is therefore a mechanism to further maximize the outputs and accelerate the impact of the overall Musa improvement effort. The programme is an innovative mechanism to bring together research carried out both within and outside the CGIAR, creating new partnerships between National Agricultural Research Systems (NARS) and research institutes in both developing and developed countries. The formation of such partnerships will also contribute to strengthening the capacity of NARS to conduct Musa-related research. The major thrust of PROMUSA is to develop a wide range of improved banana varieties from which growers worldwide can select those most suited to their needs. The programme brings together conventional breeding based on hybridization techniques with genetic engineering and biotechnological breeding approaches. This broad-based genetic improvement effort is supported by research being carried out on specific pests and diseases within the various PROMUSA working groups. An efficient mechanism for evaluating new varieties produced within the framework of PROMUSA is also an essential component of the programme. been recommended to provide the participants with a protocol to sample, establish and maintain the collection. INIBAP was mandated to collaborate with CIRAD in the development and distribution of the technical information required. The establishment of a national collection should be promoted and facilitated through the organization of a training course; especially for those countries that develop breeding programmes, but also where disease resistant hybrids of banana are used on an industrial scale, and where the high diversity of Musa would have originated similar diversity in the pathogens.The study of the genetic population structures of Mycosphaerella leaf spot diseases is already on going at national, regional and international levels. However, the group recommends increasing the number of countries involved at the national level, which will allow refinement of both regional and international studies. Both biological (morphological) and molecular determinations have been recommended to improve the understanding of the different population structures. The sampling protocol and methodology should be standardized and the recognition of the different species facilitated through the development of a technical factsheet to be widely distributed. INIBAP and CIRAD agreed to work together in the preparation of this information which should include several detailed illustrations of the different pathogens and their anamorph stages. This information will also become part of the IMTP guidelines. The development of more molecular markers as SSR and CAPS should allow the study of the different populations to be refined. The recommendation to include partners from south and southeast Asia made during the last global meeting of PROMUSA in Bangkok was reiterated by the participants who strongly suggested that the INIBAP regional office for Asia and Pacific strengthen and facilitate any exchange between Asian partners and the rest of the PROMUSA community.The pathogenicity of the different strains should be approached using either the in vitro or in vivo inoculation systems. However, it is recommended that the different methodologies that currently exist be standardized. The methodology for the in vitro inoculation on leaf fragments developed at CIRAD should be distributed, together with the methodology used to isolate, cultivate and produce the inoculum of the different pathogens. INIBAP and CIRAD have been requested to compile, in a single technical document, all the different informa-tion already published on these different methods.The need of new sources of resistance to Mycosphaerella leaf spot diseases has been identified several times in the past. Collecting missions in Indonesia, north India and Vietnam have already taken place but information has only been provided on the characterization of the resistance of the different materials collected. The PROMUSA working group recommended that INIBAP help to gather any information already available. The group also recommended to stimulate the characterization of existing collections where M. eumusae has already been reported together with other Mycosphaerella spp. e.g. MARDI, in Malaysia. In order to facilitate the screening, the group suggested using the 'severity index' as the unique parameter to detect any source of resistance. This information should allow the definition of the different reference clones needed to evaluate the resistance to Eumusae leaf spot disease.The 'severity index' will also be used to evaluate the PROMUSA segregating populations hosted at CORBANA.Several leaf fungal diseases have been reported on Musa and other related species. The group recommended the development of specific diagnostic tools according to the three main species of Mycosphaerella pathogen on Musa: M. fijiensis, M. musicola and M. eumusae. The achievement of these diagnostic tools will remain within the development of a worldwide collection of Mycosphaerella isolates; the morphological description of all the different Mycosphaerella subspecies associated with banana leaves and the development of primers species-specific as microsatellites and ITS-sequences and their test on the worldwide collection of Mycosphaerella isolates.It is therefore suggested: • to develop diagnostic tools to distinguish the main pathogens and assess currently available molecular methods for specificity, • to develop a manual with descriptions of symptoms and morphological characters, • to develop protocols for collection and analysis of samples, • to transfer to and train PROMUSA participants on the different technologies required (collection and sampling, monoascosoporing cultures and molecular markers).Significant changes in the levels of resistance to Sigatoka and black Sigatoka diseases have been reported in Australia, India and Cuba. However these may exist just because of high inoculum loads. Thus, changes in pathogen populations should be distinguished from particular epidemiological effects. Therefore, the group recommended studying the changes in pathogen populations in response to selection pressure from new banana genotypes resistant to Sigatoka diseases. It is essential to monitor changes in pathogen populations in areas where new resistant hybrids are being grown on a large scale. A special recommendation towards the development of specific trials in Cuba has been made. Two different aspects of the durability of the resistance need to be addressed: the genetic drift of the pathogen resistance and the selection effect within the pathogen population. Participants in PRO-MUSA Sigatoka working group recommended:• the selection of areas where resistant hybrids have been grown for a long time (e.g. Cuba) and to follow the evolution of the pathogen populations, isolating Mycosphaerella strains on resistant and susceptible cultivars or hybrids, • the development of molecular markers linked to the pathogenicity of the fungal strains (molecular markers will inform on the genetic drift when pathogenic evaluation will be related to the selection effect), • the quantification of the selection pressure over the time, and • the study the breakdown of resistance by in vitro testing.M. eumusae is currently limited in extent throughout most of Asia, although there is some evidence that the pathogen may have reached Africa. The dynamics of the disease are not fully understood. Some projections indicate that this disease will become more important than black Sigatoka. In order to prepare adequate disease control strategies, a detailed knowledge of the epidemiology of this pathogen is urgently required. To address the epidemiology of the different Mycosphaerella spp. pathogens on Musa, the group recommended:• the collection of disease incidence data from the field and literature,• the development of methodologies to understand the mechanisms of spore release and spore survival in the air at laboratory level, and • the clarification of laboratory data at plantation level and to assess the potential for windborne dispersal (as opposed to further spread of the disease by the transfer of inoculum).The genetic approach has been shown to be extremely powerful when studying hostpathogen interactions in some pathosystems (such as Magnaporthe grisea). This approach does not require the identification of pathogenicity factors a priori and includes the study of gene expression during infection (differential display, DNA chip, SSH, etc.), production of pathogenicity mutants, comparative genomic and gene function validation techniques.Here again, the PROMUSA Sigatoka working group ratified the recommendation made in the framework of the \"2 nd International workshop on Mycosphaerella leaf spot diseases of bananas\" to study: \"the development of genetic and molecular biology tools for M. fijiensis in collaboration with M. graminicola groups as well as to launch a genomic initiative to access to genomic tools (EST collection, physical map, genome sequence) and set up a genomic-wide comparison of M. fijiensis to M. graminicola\".The group recommended to develop an international core collection of M. fijiensis, M. musicola and M. eumusae. The different strains should be conserved as fungal mycelia and DNA. CIRAD was suggested to The working group in collaboration with INIBAP, through PROMUSA, looks for funds for the operation of the core group. Usually, PROMUSA working group meetings are held back-to-back with other international meetings, a cost-effective way of organizing meetings.• Identify sources of resistance, • Develop screening methods and protocols, • Agree on references/checks. The following suggestions were made:• To compile and exchange information on methods and checks. Standardizing sampling methods is a prerequisite for developing screening methods, • To have standard protocols for screening germplasm and for identifying sources of resistance, • To compile information on mechanisms of resistance, • To assess the possibility of site specific differences regarding resistance, • To develop research priorities that address the compatibility of genetic improvement with other management practices. • To consider developing IPM research priorities that contribute to the genetic improvement of the banana, Certain institutions whose research interests go beyond genetic amelioration, raised the possibility of establishing the working group as a separate entity from PROMUSA for fear of being restricted by the latter's mission. In the end, everybody agreed there would be a core working group on activities related to genetic improvement, but that the general membership would include policy makers and all those working on banana weevil (including its biology and status as a pest, control methods and technology transfer). It was also suggested to create a list-server to facilitate information exchange on all aspects of banana weevil research.The fact that PROMUSA focuses on genetic improvement does not mean that other crop protection research activities, e.g. pheromones and entomopathogens, are less important. These should be addressed more efficiently as researchers benefit from the multidisciplinary dynamics created by PROMUSA.Formation of the core group -This group should include scientists who actively contribute to genetic improvement, e.g. breeders and scientists working on host plant resistance, mechanisms of resistance, hybrids resistant to weevil, sources of resistance, relevant genetic studies, conventional breeding, biotechnological methods and screening methods.It was not felt necessary to split this group into scientists working on plantains, bananas or other banana types. For the time being, the group can include anyone working on any aspect of crop improvement of all bananas and plantains.It was suggested that the group adopt the same procedures for forming this group as those used by other working groups. It was agreed that: • Members who were present at this meeting form the working group, • A convenor should be elected to take charge of the working group, • The convenor should organize a meeting within the coming year to work out the way forward. In Cameroon, bananas and plantains are a major staple food for a large proportion of the population. A total of 1.7 million tonnes are produced annually. These crops are threatened by a wide range of pests and diseases among which the banana borer weevil (Cosmopolites sordidus) is the major insect pest. For more than six decades, investigations have been carried out on this pest but emphasis was given to testing insecticides that satisfy the needs of large-scale commercial banana plantations. It is only recently that studies on integrated control options were initiated in order to develop control strategies that could also be used by resource limited farmers. This report presents the activities carried out in Cameroon over the past ten years on the banana borer weevil.Four weevil species are found in the banana and plantain producing areas of Cameroon:Cosmopolites sordidus (Germar), Polytus mellerborgi (Boheman), Metamasius hemipterus sericeus (Olivier) and M. hemipterus (L.). C. sordidus seems to be the only weevil of economic importance in banana and plantain plantations (Fogain 1994, Ysenbrandt et al. 2000). The insect is found in all banana and plantain producing areas in Cameroon (Fogain 2001). A survey carried out in all the banana and plantain producing areas showed that the percentage of occurrence of C. sordidus in Cameroon varies between 50 and 90%, and that 82.5% of the farmers are aware of the problem and capable of recognizing weevil damage (Ngamo and Fogain 1998). Research on the population dynamics of the weevil in two of the most important production zones indicates that higher populations are observed beween August and September. However, this result need confirmation.In commercial banana plantations, chemical control, the use of clean planting material, and weevil habitat management are the most common methods for controlling weevil populations. Development of alternative control measures and of an integrated pest management strategy are highly recommended for resource limited small-scale farmers, the major producers of plantain.At the beginning of the 70s, weevil populations were efficiently controlled with Kepone (Chlordecone) in Cameroon's commercial banana farms. Between 1975 and 1983, the withdrawal of the product from the market caused a significant increase in weevil populations because of its replacement with HCH and other less effective insecticides, like Dursban (chloropyrifos-ethyl) and Primicide (pyrimiphos-ethyl) (Kehe 1985). The rapid decline of banana production was halted by the arrival on the market of Curlone (Chlordecone) in the early 90s. With one or two applications per year, weevil populations where effectively controlled. But the product was soon withdrawn from the market because of its limited degradability. Regent (fipronil) later came on the market, allowing efficient control of weevil populations with two or three applications per year. In Cameroon, this prod-uct is still the only efficient insecticide used in commercial banana farms. However, its continuous application will probably produce resistant weevil populations in the near future. Therefore, it is recommended to use it alternately with nematicides, like Counter (terbuphos) and Furadan (carbofuran), that have insecticidal activity and can be used when populations are relatively low. In the Moungo department, the threshold for treatment in industrial banana plantations is when 5% of the 20 sampled mats per hectare are attacked, based on the method proposed by Vilardebo (1973). Other insecticides with interesting properties are: tebupyrimphos, athiamethoxam, cartap and imidacloprid. Timely chemical control is an efficient way of knocking down adult weevil populations in commercial farms, but is too expensive for the majority of resource limited farmers and has unfavourable side effects on beneficial nontarget organisms. According to a survey conducted in southwest Cameroon, 57% of smallholder farmers said they did not use pesticides (Chantelot 1993). Forty-three percent, mostly in mixed plantain-cocoa plantations, treated suckers before planting and 87% used insecticides generally referred to as 'gabaline', insecticides used against timber or cocoa pests and which include lindane (HCH), Dursban (chloropyrifos-ethyl) and methylparathion. Three percent of the farmers who treat suckers before planting use a nematicide with insecticidal activity, such as Mocap (Ethoprophos), and 10% use other products (Chantelot 1993). Another survey in west, southwest, central and south Cameroon revealed that only 11% of smallholders use pesticides, 57% do not use anything and more than 32% use ashes because they believe it controls weevils (Ngamo and Fogain 1998).It is important to plant an uninfested field with clean planting material which can be obtainedCIRAD, Guadeloupe, expressed the desire to participate in agronomy and biotechnology related activities and to coordinate activities in Guadeloupe and Montpellier. Spanish organizations will also provide support to the working group.CORPOICA, Colombia, will contribute to the screening of cultivars for resistance to banana weevil and nematode. CORPOICA will provide support for screening methods and could also provide a weevil biotechnology specialist (Consuelo Castrillon).CORBANA, Costa Rica, will provide support for screening methods and evaluation of germplasm.EMBRAPA, Brazil, was not represented but may be interested in conventional breeding, biotechnology and screening for local stress. Needs to be contacted.FHIA, Honduras and EMBRAPA will be contacted to find out their interests (Marline Fancelli).ITSC, South Africa, proposed to screen new banana varieties, especially 'Cavendish' (Schalk Schoeman). The University of Pretoria will supervise students conducting research in banana biotechnology.CARBAP will screen for resistance to weevils, nematodes and black Sigatoka (Roger Fogain).IITA has a breeding programme on highland bananas and plantains. IITA works very closely with NARO and banana networks in East and West Africa. IITA is interested in mechanisms of resistance, as well as conventional and biotechnological methods for developing resistance (Cliff Gold).It was proposed that Dr Cliff Gold of IITA be selected as convenor. He has worked extensively on banana weevil, speaks both English and Spanish and has access to communication facilities. He can easily coordinate the preliminary activities. Therefore Dr Cliff Gold was nominated and unanimously approved.from weevil-free plantations or tissue culture facilities. Ninety-five percent of smallholders practice paring of suckers before planting (Chantelot 1993), but since the availability of good planting material is a major limitation in Cameroon, infested suckers of minor quality are often planted. Residual corms in the soil should be destroyed and post harvest residues slashed in order to prevent the multiplication of weevils. Weeding should be done regularly in order to avoid development of a favourable humid weevil habitat. In smallholder farms, habitat management is neglected because labour is limited or rented labour is not productive enough. Weeding is minimal (two to three times a year) and herbicide application is rare. A minority of farmers prop with bamboo even though the practice can give good results with minimal investments. In a survey carried out between February 1997 and March 1998 of 240 plantain plants in eight smallholder farms, plant losses due to nematodes, weevils, and water and nutrient stress represented 60% of the losses, of which 37% were due to toppling or falldown (mainly at the beginning of the rainy season, due to violent winds) and 29% were due to breakage of the pseudostem (mainly at the end of the dry season due to water stress) (Anonymous 1998). More than 30 % of smallholder farmers use household ashes at planting because they believe it reduces damage to the corm (Ngamo and Fogain 1998). It is not clear whether ashes have an insecticide or merely a fertilizer effect. Under laboratory conditions, ashes have a repellent effect on adult C. sordidus, but the toxicity to adults is quite low (Messiaen 1999). Commercial banana plantations are renewed every five to six years. During the fallow period, residual corms are usually destroyed by local women who use the fallow field for food crop production. Tissue cultured plants are treated with Regent5G (fipronil) or Counter10G (terbufos) at planting and two or three times a year. Crop hygiene (weeding, herbicide application, slashing of residual pseudostems and toppled mats) as well as propping and guying are commonly practised.At CARBAP, research on biological control using the entomophagous fungus Beauveria bassiana started in 1994 with the discovery of local strains in Cameroon (Fogain 1994). Since then, studies have been carried out under controlled conditions to test the efficiency of the strains and the possibility of mass production for field trials. Three strains of Beauveria bassiana, isolated from infected weevils caused 92% mortality after nine days under laboratory conditions. Research is presently carried out on the maintenance of viability with regards to delivery systems and the feasibility of mass production for farmers or economic agents in Cameroon. Entomopathogenous nematodes have been isolated from soil samples collected in Cameroon using C. sordidus larvae.Dipping suckers in a 20% neem (Azadirachta indica) seed solution at planting protects the young suckers from weevil attack for several months, but a crown application three times a year is not effective in reducing damage (Fogain and Ysenbrandt, 1998). It achieves this result by reducing oviposition, through its repellent effect on adult weevils, and by blocking egg hatching (Messiaen 1999).Trapping using pseudostem traps does not always reduce weevil populations, depending on the cropping system, the level of weevil immigration from neighbouring infested plots, the number of traps placed and the initial population. In Cameroon, trapping does not seem to be a viable control option in smallholder farms because of the unrealistic amount of pseudostem and labour needed, and because of weevil immigration from adjacent plots. Testing of a mass trapping system using ramp traps baited with sordidin, an aggregation pheromone, indicated that the traps were not attractive enough to constitute a viable control option in industrial banana plantations, but additional research is needed to assess whether the attractiveness can be improved with another type of trap and kairomones. In smallholder farms, pheromone mass trapping does not seem to be a viable option for controlling weevil populations because of problems of storage and costs (Messiaen 2000a).Screening for resistance to the banana borer weevil at CARBAP stated in the 1994 with the discovery of the field resistant 'Yangambi km5' and of the highly susceptible clones of the plantain subgroup (Musa AAB) compared to the 'Cavendish' (Musa AAA) (Fogain and Price 1994). Since then, techniques for early screening in the field and under controlled conditions have been refined. In a recent screening, more than 80 varieties were tested. Several varieties, including CARBAP hybrids, have been selected for enhanced screening in the field. Results of preliminary screening show a large variety of responses to weevil attack between and within genomic subgroups (Messiaen 2000b). No genotypes are more susceptible to weevil attack than the ones in the plantain subgroup. Preliminary results point to differences in larval development. If the results are confirmed in the field, it will be possible in the short or medium term to develop hybrids partially resistant to C. sordidus.Significant information has been gathered over the past ten years on the distribution and population dynamics of the weevil. Despite gains in knowledge on the dynamics of C. sordidus in the Fako and Mungo divisions (littoral and southwest Cameroon), investigations are needed for other provinces, such as the major plantain producing areas of the centre and south (Anonymous 2000). The insect was found to be present everywhere in the country where bananas and plantains are produced. A larger spectrum of insecticides is available, but mainly to commercial growers. Several insecticides from different chemical groups are now available and can therefore be used in rotation to avoid the development of resistance to C. sordidus. As for botanical and biological control agents, neem (A. indica) and the entomopathogenic fungus B. bassiana show great potential for weevil control. But field trials are needed to confirm greenhouse results. Sources of resistance to the weevil have been identified and breeding programmes can now use them to develop genotypes resistant to the insect. Research on other non-chemical control methods, such as cultural control and the use of pheromones, continues.The biology and management of the banana weevil, Cosmopolites sordidus in South Africa P. Govender 1 and A. Viljoen 2 The banana weevil, Cosmopolites sordidus, introduced in South Africa about 30 years ago, is the most important insect pest of banana, causing economic losses in the Mpumalanga and the south coast of Kwazulu-Natal regions. Collectively this area represents about 78% of the total 12 078 hectares under commercial banana production in the subtropical pockets of South Africa. The weevil has a limited potential to migrate from its current distribution area unless it is transferred with infected planting material. Information on the life cycle appears to be consistent with published literature; the total developmental period being about 33 days. Adults emerge during spring and late summer, and their nocturnal activity increases during or after rainfall. Females generally lay one egg per week from late August to February but this number can increase during optimal environmental conditions and low pest densities. Adults have a life span of about two years. Although weevil numbers are low in the winter months (May to July), they increase rapidly in spring and early summer (August to November). Weevils are monitored using pseudostem traps at a density of 50 traps/ha. Economic threshold values are 1-2 adults/trap/week and 10 or more larval tunnels/corm. Weevils are strongly attracted to the 'Williams' and 'Chinese Cavendish' cultivars. A tentative recommendation algorithm was developed for the management of C. sordidus in South Africa. It integrates standard cultural control, trapping, biocontrol and chemical control. Two local entomopathogenic fungi (Aspergillus flavus and Beauveria bassiana) have been isolated and tested in South Africa but their host specificity and pathogenicity requires further investigation. Various nematicides with insecticidal properties have been tested in limited field trials and currently only aldicarb 15% GR, at a rate of 2.03 to 3.00 g a.i./planting station, is registered for use against the banana weevil. The banana weevil is an important constraint on the production of East African highland bananas and plantains. The larvae attack the corm, reducing nutrient uptake and weakening the stability of the plant. Attack in newly planted banana stands can lead to crop failure. In established fields, weevil damage can result in reduced bunch weight, plant loss, mat die-out and shortened stand life. In Uganda and Tanzania, it has been implicated as a primary factor in the decline and disappearance of cooking bananas in traditional growing areas. Uganda has ranked banana weevil as the most important biotic constraint on highland banana production. Salient features of the weevil's biology are restricted host range (Musa and Ensete), long life span (up to 4 years), low fecundity (1-3 eggs/week), 1:1 sex ratio, nocturnal activity, uncommon flight and limited dispersal capability. The weevil most commonly enters new fields through infested planting material. Because of populations building up over time, pest problems are more pronounced in older plantations. In one trial in Uganda, yield loss increased from 5% in the plant crop to 47% in the third ratoon. This loss was attributed equally to plant loss and bunch weight reductions. By the 7 th cycle of the second trial, 35% of the mats had died out in weevil-infested plots compared to 2% in controls. Overall yield losses were 50% for the trial. Although pesticides can be an effective control method, in Uganda the weevil has developed resistance to one chemical. IITA and the Ugandan National Banana Research Programme work closely together on cultural and biological controls, and on host plant resistance. Clean planting material is an important means of keeping weevils out of new plantations, but the effect normally disappears within a few crop cycles. A one-year trapping study showed some positive effects on population reduction but this control is beyond the resources of most Ugandan growers. Current emphasis are on the use of neem, endemic ants, microbial control (i.e. Beauveria bassiana and endophytes) and host plant resistance. Available data indicate that all highland banana clones are susceptible to weevil. However screening trials suggest that many resistant Musa clones do exist and that antibiosis is the predominant means of resistance in these clones.Luís Nuno and V. P. Ribeiro Direcção Regional de Agricultura da Regional Autónoma da Madeira -Portugal Direcção de Serviços de Produção Agrícola/Divisão de Bananicultura, Centro de Bananicultura -Lugar de Portugal Located between 32°38' north and 16°54' west, the island of Madeira lies about 800 km from the coast of Morocco. Banana plantations occupy nearly 850 hectares of the island and are exposed to a subtropical climate. In Madeira, the culture of banana started in the 18 th century. The most widely grown variety on the island is the 'Pequeña enana', introduced in 1842.Although its presence has been known for some time, the banana weevil, Cosmopolites sordidus, is not a major source of problems. The first identification dates back to the 19 th century. In 1992, started the installation of spot irrigation systems and the use of varieties produced in vitro. In both instances, this has been accompanied by outbreaks of C. sordidus. In one case (spot irrigation), outbreaks increased because farmers stopped doing certain things like burying plant residues, a practice which helped control the pest. In the other case, available data suggest that C. sordidus prefers plants produced in vitro. Even though they occur in all plantations, these problems are more frequent in the above-mentioned situations. For some years, banana growers applied to the base of the pseudostem an insecticide (Baytion) which has foxime as an active ingredient. They also used a pirimifos-ethyl based product (Bullit) but stopped when these insecticides were banned. They currently apply etoprofos (Mocap 10G) directly on the soil. A recent innovation is the use of pheromone traps, produced by N.P.P. Calliope (France), to fight C. sordidus. The first results are promising.Ruth Torres del Castillo and Clemente Méndez Hernández ICIA, Tenerife, Canary Islands, SpainThe agricultural service of the government of Tenerife Island (Cabildo) did a series of surveys to determine the extent and severity of damages caused by Cosmopolites sordidus Germar. Carried out between 1996 and 2001, the surveys were repeated every three years, depending on the study area. Peeled off pseudostem double-disc traps and those with a horizontal section of the corm were highly correlated (R2=0.93). The distribution of C. sordidus in relation to the type of irrigation, the altitude of the plantation and the variety used ('Pequeña enana' or 'Gran enana') was also studied. Time trends were done to see the evolution in the distribution of this pest and the damages it causes.Sección de Fitopatología y Entomología, Dirección de Investigaciones de CORBANA S.A., Costa Rica Chemical control and cultural practices are the base for the management of banana weevil in plantain and banana plantations in Costa Rica. Usually the damage of the pest is more important in plantains (Musa AAB) than in bananas (Musa AAA), due to differences in varietal susceptibility and in crop management. We are carrying out studies on the ecology and biology of the pest, and on methods for chemical, biological, ethological and cultural control. The combination of some of these methods could be the best alternative for the management of the pest. Collaborative research is being conducted in Uganda to assess the microbial control potential of Beauveria bassiana (Balsamo) Vuillemin (Hyphomycetes) for the banana weevil, Cosmopolites sordidus (Germar), (Coleoptera: Curculionidae). Since the early 1990s, isolation, characterization and pathogenicity studies have come up with a selection of indigenous isolates of B. bassiana that have good growth and production traits, causing 50-100% weevil mortality within 10-21 days after inoculation, depending on the isolate. In small-scale field trials conducted at Kawanda Agricultural Research Institute, one B. bassiana isolate (code G41), which showed high pathogenicity to C. sordidus as well as superior growth and sporulation compared to other isolates, was tested. Three methods of delivering B. bassiana, namely (i) application of the fungus on topsoil around the base of the banana mat (ii) application of the fungus with pseudostem and disc-on stump traps and (iii) application of the fungus to banana planting suckers, were evaluated. Treating banana suckers with a B. bassiana dry maize culture formulation and a soil-maize based formulation (2.3 x 10 12 conidia/planting hole), reduced weevil damage by 20-30% within a period of eight weeks after planting in holes dug in a 2 to 3-year-old banana field of local EAAH cooking cultivar. Dead C. sordidus adults and larvae with B. bassiana fungal growth were observed in the treated suckers indicating immature stage infection. When the maize and soil-based formulations of B. bassiana were applied beneath the pseudostem and disc-on stump traps, it was observed that the moist conditions under the traps, in addition to attracting weevils, also provided a favourable environment for extra sporulation of B. bassiana and this enabled the fungus to remain potentially infective. B. bassiana cultures collected from the field traps in the first five weeks after application were highly infective causing 60-100% weevil mortality in 14 days, but the infectivity of the fungus was significantly reduced in the wet season; likely due to contamination by other soil micro-organisms. The maize culture formulation (2 x 10 15 conidial/ha) and maize-soil based formulation (2 x10 14 conidia/ha) applied at the base of the mats reduced the adult banana weevil populations by 30-50% and kept them at lower levels than in the untreated plots. The treated plant also showed reduced weevil damage and up to 16% B. bassiana disease infection was observed in dead weevils in the field. Previous studies have demonstrated that good potential exists for the use of B. bassiana for microbial control of the banana weevil. The collaborative team, composed of scientists from the International Institute of Tropical Agriculture (IITA), the National Banana Research Programme of the Uganda National Agricultural Research Organization (NARO), CABI Biosciences UK and University of Reading is undertaking further research into the mass production and formulation of B. bassiana and exploring other delivery systems of B. bassiana for integration with other control measures under farmers' conditions. Research is geared to developing economically viable mass production, formulation and delivery systems that will overcome the problems associated with field fungal efficiency, persistence and transmission. Further research on the ecological relationships between banana weevils and entomopathogens will also be undertaken to understand the conditions under which B. bassiana is likely to be most effective in controlling this pest. This include studies on the behaviour of the weevil, which might influence the likelihood of the insect contacting the pathogen; the biotypes in the Cosmopolites sordidus species, which might exhibit different susceptible levels to the pathogen; and pathogen viability and virulence under aerobic (ordinarly pseudostem traps) and anaerobic conditions such as in the semio-chemical-based trapping systems. We acknowledge funding from the Rockefeller Foundation, DFID and BMZ in support of this work.Universidad Autónoma, Barcelona, Spain Entomopathogenic nematodes (Heterorhabditis spp. and Steinernema spp.) are used in biocontrol against different insect pests in soil and cryptic habitats. They are symbiotically associated with bacteria of the genera Photorhabdus and Xenorhabdus, respectively. Nematode dauer juveniles, harbouring cells of their specific bacteria in their intestine, search for insects in the soil. After penetration in the host insect, they release their symbionts. The bacteria multiply and produce suitable conditions for nematode reproduction in the dead insect. After about two weeks, dauer juveniles emigrate from the cadaver and search for a new host. The use of entomopathogenic nematodes for the control of Cosmopolites sordidus should now be economically feasible. Production and formulation techniques have been improved to provide these nematodes to growers at a cost equivalent to or lower than the one of chemical insecticides. Application of entomopathogenic nematodes requires less labour than insecticides, avoids the problems of insecticide resistance and has little or no adverse effects on the environment. However, for reliable results, it is necessary to improve the application technique, and to select the appropriate species and strains of entomopathogenic nematodes. Finally, various strategies for the control off Cosmopolites sordidus using entomopathogenic nematodes are discussed.Dennis Alpízar M.Estación Experimental Los Diamantes. Ministerio de Agricultura y Ganadería. Guápiles, Limón, Costa Rica In Costa Rica, insecticide-nematicides are commonly used to control Cosmopolites sordidus. Pseudostem or corm traps are also used while the utilisation of the aggregation pheromone Cosmolure®, introduced at the end of the 90s to control the banana weevil, is still a new agricultural practice in the country's banana and plantain plantations. The objective of this study is to compare the effect of using or not using, under the same conditions over two years, two insecticidenematicides: terbufos (four applications) and etoprofos (one application). After two years the results show, using the Fisher t-test (0.05), that the 'weight of the bunch' was slightly but significantly higher in the non-treated plots while the 'number of nematodes (Radopholus similis) in the functional root of the sucker' was slightly lower in the treated plots. The other variables were not statistically different.The cost of applying the insecticide-nematicide was $US 1200 per hectare for the twoyear duration of the study.A. Padilla Cubas, F. García del Pino, L.V. López Llorca and A. Carnero Hernández ICIA,Apartado aéreo 60,38080 La Laguna,Tenerife,Islas Canarias,Espaa In the Canary Islands, Cosmopolites sordidus (Germar) is the most important pest in plantations of banana plantain. Given the results of chemical treatments, alternatives are sought to control the weevil. We therefore sampled soils, cultivated and not cultivated, in the province of Santa Cruz de Tenerife looking for parasitized organisms. Using 'Galleria mellonella' traps, we especially searched for entomophagous nematodes and fungi. Entomophagous nematodes were found in two sampling points from which we isolated Heterothabditis spp. and Steinernema spp. As for entomophagous fungi, we isolated Aspergillus flavus, Beauveria bassiana, Metarhizium anisopliae and Paecilomyces spp. Verticillium lecanii was isolated form white flies collected in different localities. We characterized the morphology of the fungi and studied their germination and sporulation capacity, their production of biomass and their behaviour under various naturally occurring conditions of humidity, temperature and pH. We also studied their enzymatic activity: quitino, amylo, proteo, lipo and pectinolytic. We also conducted biological assays using 'Galleria mellonella' and, based on the results, we inoculated C. sordidus using two methods. Finally, we evaluated the interactions between these isolates and Fusarium oxysporum, the main pest of banana. We studied the use of ornamental plant vegetal residues from nurseries to produce inocula of antagonistic fungi, including entomophagous fungi. Seeds of Phoenix dactylifera turned out to be excellent for the production of Beauveria bassiana. Scanning electron microscopy revealed a very porous substrate, a feature which facilitates fungal development and sporulation. A soil-based formulation of B. bassiana can sporulate and overcome fungistasis. Thanks to this type of seed, the fungus can survive and maintain itself in the soil during at least three months. During bioessays, the mixture infected a pest of palm trees (Carpophilus dimidiatus) similar to the weevil. Moreover, B. bassiana can colonize the petioles of P. dactylifera. We think that such endophytic behaviour is useful in controlling pests like the weevil. The research focuses on integrated pest management along the same lines as CARBAP. The results presented focus on the combination of two types of synthetic pheromones; the use of antomopathogenous bacteria and nematodes; and the efficiency and limits of chemical insecticides. Under laboratory conditions the development of the insect and its non-preference for feeding and oviposition will be studied for the same genotypes in order to identify the types of resistance involved in the interaction between the weevil and the banana plant. The duration and viability of the larval and pupa phases, the weight of the pupa after 24 hours and the number of adults with defects, will be noted. Tests for attractiveness and consumption will be carried out. Analyzes will be done to identify the presence of attractive/repellent substances, as well as phago stimulants and/or phago deterrents. Rhizome hardness will be evaluated using a penetrometer. The banana weevil (Cosmopolites sordidus Germar) is probably the most important pest affecting the production of banana and plantain. Attack by the weevil results in severe crop losses from plant toppling, snapping, death and reduced bunch weights (INIBAP 1986). Pesticides are effective, but uneconomical for small-scale subsistence farmers.In addition, the weevil is resistant to a wide range of insecticides and although cultural controls may help, labour and material requirements often limit their adoption (Gold 1998).Host plant resistance shows potential for the long-term control of the banana weevil on small-scale farms, within an integrated pest management perspective (Seshu-Reddy and Lubega 1993). However, the development of weevil resistance in banana is still in its infancy and breeding programmes have only recently included weevil resistance as a criteria for introgression into cultivated Musa. The cumbersome nature of resistance screening methods has made work on weevil difficult, slow and at times expensive. Lack of understanding of resistance mechanisms and their associated genes, coupled with long generation times, triploid sterility of most edible cultivars and poor seed set due to incompatibility, have limited conventional breeding efforts on Musa.The literature on weevil resistance in Musa reviewed by Pavis and Lemaire (1997), Kiggundu et al. (1999) and Kiggundu (2000) suggests that antibiosis is the key resistance mechanism. Sources of resistance have also been found in the Musa germplasm tested by Fogain andPrice 1994, Lemaire 1996, andKiggundu et al. (in press).From a screening trial conducted over four crop cycles at the International Institute of Tropical Agriculture-East and Southern Regional Center (IITA-ESARC), in Uganda, we found that East African highland bananas (EAHB) (AAA-EA) and plantains (AAB) were the most susceptible. The beauty of genetic engineering is that genes from several sources can be exploited and that these can be transformed using a gene pyramiding strategy. However, a great deal of information about the complex nature of weevil resistance is missing and molecular marker analysis can assist in genetic analysis and mapping. Opportunities also exist for quickly developing weevil resistance through genetic transformation.The The objectives of the project are: 1) to optimize the use of pheromone to control the weevil; 2) to evaluate the relation between the extent of the damages caused by the weevil and yield losses;3) to determine weevil inter and intra-population variability in relation to biological control; 4) to identify and study the behaviour of the entomophagous organisms attacking the weevil. Our participation in this project consists in determining weevil inter and intra-population variability among the populations affecting the banana plantations of Tenerife, Gomera and La Palma, in order to provide information which could help in controlling this pest, such as the origin and the dispersal patterns of these weevils.Detecting DNA polymorphism will be done using the randomly amplified polymorphic DNA technique, following the methodology used to study the population genetic of another species of Curculionidae, a pest of sugarbeet in Andalusia (Taberner et al. 1997, J. Mol. Evol. 45:24-31).We also plan to identify and characterize the digestive enzymes of the weevil, and to conduct bioassays with protease inhibitors in order to determine their effect on the survival and development of this pest. This information is necessary for the eventual production of transgenic banana plants expressing defence proteins, a possibility which would offer new means of controlling the weevil. Preliminary studies suggest that larvae and adults possess a complex proteolytic system which includes aspartyl, cysteine and serine endoproteases as well as amino and carboxypeptidases. Once the characterization of digestive proteases is finished, we will study their interactions with specific inhibitors in order to determine which inhibitors, or combination of inhibitors, could be incorporated by genetic manipulation in potentially resistant banana plants.XV ation of chimerism and developing flow cytometry protocol.Several young students benefited from this CRP in completing their master and PhD programmes in Israel, Czech Republic, and Belgium. Some of the participants presented their results in international conferences. A total of 51 research papers have been published in conference proceedings and international refereed journals.Many international trainees received training on several aspects of banana tissue culture, molecular cytogenetics and molecular markers at KULeuven and Faculté universitaire des sciences agronomiques, Gembloux (FUSAGx), Belgium; Institute of Experimental Botany (IEB); and University of Frankfurt, Germany. The trainees came from China, Cuba, Egypt, Mexico, and Rwanda. The outcome of these trainings was very successful. For example, a Cuban trainee was successful in establishing new somatic embryogenic banana cell suspensions from Cuban plant material. In addition, he successfully irradiated plant material in Cuba. In Sri Lanka, 20 persons from countryside were given training in tissue culture technology for mass production of banana. Post-graduate training on indexing of banana viruses was organized.Flow cytometry facilities were established at the International Institute for Tropical Agriculture (IITA, Nigeria) and The Malaysian Institute for Nuclear Technology MINT, Malaysia). The transfer involved staff training in the Institute of Experimental Botany (IEB, Czech Republic) and expert visit.1. Detection of DNA methylation polymorphism in banana micropropagated plants with amplified fragment length polymorphism (AFLP). 2. Somatic embryogenic cell suspension cultures (ECS) were developed for several banana cultivars including plantains (AAB). Three cryopreservation techniques were developed for long-term conservation of meristems. An INIBAP technical guideline for cryopreservation of banana was published in English, French and Spanish. 3. Induced mutations generated a series of improved clones that were screened for different traits such as early flowering, reduced height, large fruit size, and tolerance to Fusarium. 4. Both Agrobacterium-mediated transformation and particle bombardment methods were used for banana transformation, and transformation rate was cultivar dependent. 5. Virus indexing procedures were transferred to Sri Lanka for indexing local banana virus strains. 6. An early screening technique was developed for Fusarium wilt using tissue culturederived plants in a double-tray system. 7. A selection system was developed against black Sigatoka disease by using Mycosphaerella fijiensis crude extract, the semi-purified, and one purified fraction (juglone). 8. Screening techniques for nematode resistance were developed in Musa under shade-house and field conditions. Aseptic cultures of Radopholus similis and Pratylenchus coffeae were established using alfalfa calli, and their pathogenicity was confirmed after greenhouse tests. 9. DNA flow cytometry was used for detection of polyploidy, monitoring of cytochimera dissociation, and analysis of karyological stability of ECS. 10. Transposon mutagenesis was explored for gene tagging, using maize Ac element, in banana genome. A substantial number of distinct mutants were generated and characterized. 11. Fluorescence in situ hybridization (FISH) protocol was developed for Musa for detailed studying of karyotypes, providing distinct chromosome landmarks, gene localization, analysis of long-range chromosome structure, and linking to physical and genetic maps. 12. A total of 28 allele-specific simple sequence repeat (SSR) markers were generated for Musa and used to detect: polymorphisms between the A and B genomes, identify hybrids, and trace back the B genome in hybrids. These markers are now used within the CRP and worldwide. A total of 24 locus-specific, highly polymorphic SSR markers were also produced for Mycosphaerella fijiensis to discriminate them from other species.A. James, S. Peraza-Echeverria, V. Herrera-Valencia and L. Peraza-Echeverria Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico.The extent of DNA methylation polymorphisms was evaluated in leaf tissue of micropropagated banana (Musa AAA cv. 'Grande naine') derived either from the vegetative apex of the sucker or the floral apex of the male inflorescence using the methylation-sensitive amplification polymorphism (MSAP) technique, which utilizes the restriction isoschizomer pair Msp I and Hpa Il, whose ability to cleave at the sequence 5'-CCGG-3' is affected by the methylation state of the cytosines. In all, 465 fragments, each representing a recognition site cleaved by either or both of the isoschizomers were amplified using eight combinations of primers. A total of 107 sites (23%) were found to be methylated at cytosine in the genome of micropropagated plants. The highest number of DNA methylation polymorphisms was detected in plants micropropagated from the male inflorescence with 14 (3%) and the lowest in plants micropropagated from the sucker with 8 (1.7%). These differences were not statistically significant. In leaf tissue of conventionally propagated plants DNA methylation polymorphisms were not detected. Micropropagated plants were relatively hypermethylated in comparison to conventionally propagated plants, with some bands being methylated in all micropropagated plants but non-methylated in all conventionally propagated plants. These results demonstrated the usefulness of MSAP to detect DNA methylation events in micropropagated banana plants and indicate that DNA methylation changes are associated with micropropagation.different banana biotechnologists. Disease resistance and improvement of fruit quality have been the focal points of most Musa breeders. However, despite growing interest in banana biotechnology, the pool of Musa genes in public databases is relatively small (of the approximately 300 accessions placed in the NCBI database less than 25% are annotated cDNA's). Our laboratory is currently employing several approaches for the identification of functional genes in the Musa genome. These include transposon tagging, 'high throughput' random mutation by ribozyme cleavage of mRNA, suppression subtractive hybridization (SSH) and bioinformatic annotation of clustered EST's. We have introduced the maize Ac transposable element into the Musa genome and followed excision and insertion of the element in numerous transgenic lines. The goal was to investigate the frequency of transposition and distribution of insertions along chromosomes. The constructs we have used include an Ac element fused to GUS reporter under the 35S promoter. PCR analysis of a variety of mutants revealed that most carried a chimeric pattern with regard to expression of the foreign genes. Consequently, only a few transgenic lines (tissue cultured siblings) showed detectable differences in the banding pattern on Southern hybridization blots. Attempts were made to stabilize the Ac element following a limited number of transpositions, by silencing the gene encoding the transposase enzyme after excision. Differentially expressed genes, which are activated in the post climacteric phase of fruit development, were analyzed in the peel and pulp of banana fruit. Using suppression subtractive hybridization (SSH) we have isolated over 200 partial cDNA's encoding genes which are expressed during the final stages of fruit development (senescence). High throughput screening by membrane hybridization was employed for preliminary selection of candidate genes involved in regulation of the onset of senescence. Sequence analysis and blasts against GeneBank databases revealed approximately eighty non-redundant clones, which were up regulated in the post-climacteric phase. Most, but not all of these genes were up regulated, after exposure of green fruit to 1000 ppm ethylene for 24 hours. The sequenced pool of up-regulated cDNA's fall into one of three major categories: Genes involved in metabolic processes, mainly carbohydrates and lipid components. Genes involved in cellular regulation (protein kinases, transcription factors etc.). Genes involved in protection from pathogens and environmental stress conditions -metallothionein like protein, super oxide dismutase, osmotin-like protein, pathogen related proteins etc. A significant number of sequences showed no substantial homology to functional genes in the GeneBank. This project focuses on the analysis of Musa genome at nuclear and chromosomal level with the aim to understand long-range organization of Musa chromosomes and to characterize changes of chromosome structure during speciation and evolution of cultivated clones.We have used flow cytometry to determine ploidy levels of Musa accessions held at the INIBAP Transit Center (KULeuven). Flow cytometric ploidy assay involved preparation of suspensions of intact nuclei from small amounts of leaf tissue and the analysis of fluorescence intensity after staining with DAPI. Chicken red blood cell (CRBC) nuclei were included in every sample as an internal reference standard (Figure 1). From the 890 accessions analysed so far, 8.4% were classified for the first time, and 7.6% accessions exhibited other ploidy then reported previously. In 2% of the accessions, plants of mixed ploidy were detected. A reliable and highthroughput system for ploidy screening in Musa is an important outcome of the study. The use of CRBC nuclei, allowed high-resolution analysis, and the results obtained so far indicated suitability of this system for rapid detection of aneuploidy. As the materials for analysis were sent by express mail, this work demonstrates that it is possible to perform flow cytometric ploidy analysis in distant laboratories. In attempt to characterize DNA sequences contributing to structure and evolution of Musa chromosomes, we have constructed partial genomic DNA libraries in M. acuminata and M. balbisiana and screened them for clones carrying highly repeated sequences, and sequences carrying rDNA. Isolated clones were characterised in terms of copy number, genomic distribution in M. acuminata and M. balbisiana, and sequence similarity to known DNA sequences (Table 1). In contrast to many plant species where mobile elements and their remnants contribute most of the nucleotide content, our observations indicate that these elements do not represent a major fraction of the Musa genome. All repetitive sequences were more abundant in M. acuminata. As the genome of M. acuminata is larger compared to M. balbisiana, the present results demonstrate that the increase in genome size of M. acuminata was due to multiplication of some repetitive sequences. The findings of this study improve the knowledge of long-range organization of chromosomes in Musa. The availability of homologous probes for fluorescence in situ hybridization (FISH) will allow more specific mapping of rDNA sequences. A novel protocol for isolation of high-molecular-weight DNA in Musa has been developed and the work is in progress to construct bacterial artificial chromosome (BAC) library for the B genome of Musa. Availability of the BAC library will permit isolation of clones containing low proportion of repetitive DNA. Such clones will be localized using FISH and will be used to increase the number of already existing chromosome landmarks. Furthermore, BAC library will be screened for clones, which con- tain molecular markers and/or genes of interest. This strategy should result in effective integration of genetic and physical maps. Once developed, physically mapped molecular markers will facilitate map-based cloning of genes of interest including those induced by irradiation and chemical mutagenesis. Ploidy screening of Musa germplasm was supported in part by INIBAP. Banana (Musa acuminata) and abaca (M. textilis Nee) are two of the most economically important Musa species cultivated in the Philippines for fruit and fibre, respectively. These crops are both difficult to breed by conventional means and they share a number of common diseases. This report summarizes our efforts during the past five years to generate useful induced mutants of Philippine banana cultivars and to evaluate the usefulness of DNA markers in characterizing genomic alterations in the advanced generations of induced mutants. Furthermore, this report highlights the valuable contribution made by this CRP project in analyzing genetic variation in the related Musa species, M. textilis, by utilizing some of the results of the DNA marker techniques generated for banana. The highlights of our accomplishments are: • Advanced generations of induced mutants of two Philippine banana cultivars 'Lakatan' (AAA) and 'Latundan' (AAB) with promising traits were obtained from irradiation using 40Gy gamma ray and 3Gy fast neutron and subsequent in vitro culture manipulation and field evaluation. • DNA marker techniques such as RAPD, SSR and AFLP were successfully used to characterize genomic differences between the two banana cultivars used. However, only RAPD and AFLP techniques were able to detect genomic alterations between nonirradiated and induced mutants in the two cultivars. Due to better reproducibility and higher multiplex ratio, AFLP technique is preferred over RAPD technique. Hence, this technique was used to detect polymorphism between the original mutated clones and derived suckers. Silver staining procedure for SSR and AFLP analyses were routinely used.• RAPD, SSR and AFLP techniques developed for banana through this CRP project were found to be highly applicable to abaca.A number of SSR primers developed for banana gave amplification products using abaca DNA. With complimentary funds provided by a grant from the Philippine government, RAPD, SSR and AFLP analyses were successfully conducted to evaluate the genetic variation in the abaca germplasm collection of the Philippines. Comparison between morphological and molecular analyses was also conducted.N. Roux 1 , A. Toloza 1 , J. Dolezel 2 , R. Swennen Banana is the most widely consumed fruit in Sri Lanka, and is an attractive perennial fruit crop for small-scale farmers. This is due to its high economic value throughout the year. Thus, lowland rice fields in some areas are being converted for banana cultivation. Among the local cultivars, 'Embul' (Mysore, AAB) has the highest demand for cultivation. The annual production of banana is around 450 000 metric tonnes. Until recently, banana cultivation was limited to small plots, but large fields are now being established. More and more rice farmers are changing over to banana cultivation as the net profit is about four times higher than for rice, and less labour and other inputs are required. During the last six years, 2500 ha have been converted to banana cultivation (Anon. 2001(Anon. , 2002)). In addition, it was noted that the nutritional level in farmers' families has improved due to the habit of consuming more fruit. Usually, farmers do not use pesticides for banana cultivation and this has benefits for human health and the environment.Since 1990, research on banana micropropagation through shoot-tip culture, induced mutation through gamma irradiation, cell suspension cultures and somatic embryogenesis, and ploidy analysis for variation detection has been conducted by the University of Colombo. From 1995, both 'Embul' and 'Cavendish' cultivars were included in the mutation breeding programme. After irradiation of in vitro shoot tips with 45 Gy, two selections were made with shorter height and early fruiting, six PROMUSA XIX month after planting (Hirimburegama et al. 1997). Micropropagated plants of these selections were tested for stability of the characters until the second generation. The technology was perfected and is being transferred to the farmers (Laksiri and Hirimburegama 1999).Early fruiting and harvesting of micropropagated banana plants saves at least one month in comparison to traditionally grown plants that usually need eight months to flower. Thus, the number of ratoons in two years becomes three instead of the usual two, thereby increasing the income of farmers with 25% (equivalent to about US$350 per ha per year). Mass production of plants is in progress. Thus, indexing/testing of plants for viruses, i.e. banana bract mosaic (BBrMV) and banana streak virus (BSV) has become essential, since virus-free, indexed mother stocks are required for micropropagation. The causative agent of the banana bract mosaic disease was confirmed in the widely cultivated local variety 'Embul' from Sri Lanka by Thomas et al. (1996Thomas et al. ( , 1997)). This disease is more prevalent in cultivations, which are not properly managed, but the impact on the yield appears to be significant.All the currently reported disease symptoms were observed in infected plants in the field but in varying degrees. Spindle shaped purple or dark red coloured streak patterns on the pseudostem in addition to dark spindle shaped streaks on bracts were the most common symptoms of infection. Mature plants with inflorescence having black or reddish brown streaks on the outer surface of the open bracts also had streaks on the pseudostem. The splitting of the base of young suckers could also be due to other banana virus diseases.A study was done with the objective of developing a low-cost DAS-ELISA detection kit. Anti-serum for BBrMV (of Queensland Department of Primary Industries -QDPI, Australia) was tested as the coating antibody to replace the relevant component of the Agdia commercial kit. Results showed a relatively high efficiency with the QDPI antibody.Work is also in progress to make the alkaline phosphatase enzyme conjugated antibody to substitute the one of the test kit. Once the local antiserum is produced, it is expected that an effective and low cost local diagnostic kit will be developed for the routine indexing of banana plants for BBrMV. Purification of the virus extract (Thomas et al. 1997) is a limiting factor for obtaining the antigen for the antibody production process.Jorge López Torres Oxygen uptake was measured by Warburg's manometers. Based on our results, a short stature mutant ('Parecido al Rey' 6.44) was obtained. Few more mutants were selected, which were tolerant to black Sigatoka with increased yield, in some cases ('Parecido al Rey' 6.32 and 'Gran Enano' 6.44 mutants and III-2). However, these characters were unstable, probably affected by the environment, or in vitro culture conditions. Therefore, we looked for other alternative approaches for improving induced mutation rates, e.g. somatic cell suspension cultures and later mass of somatic embryogenic cells were established.From each batch of somatic embryogenic cell suspension mass cultures, 3250 to 6625 somatic embryos were obtained with 20.7% germination and 95% plant survival in ex vitro conditions. Their genetic stability is presently being tested in field conditions. The action of M. fijiensis extract on cell suspension culture resulted in large number of oxidized cells at higher extract concentrations. Damaged cells were characterized by a compact cytoplasmic content with a dark colour in its centre and leaving an empty space between it and the cellular wall. Forty-five days after incubation, more than 60% cells were found in the previous described conditions due to toxin diffusion from discs. Oxygen uptake valves had decreased normally in non treated control of all IMTP cultivars, and the maximum decrease observed in treated plants in comparison with non-treated plants was: 'Yangambi km5' (38.7%); 'Calcutta 4' (27.0%); 'Pisang berlin' (13.4%); 'Pisang lilin' (20.9%) while oxygen uptake was increased by 70% in 'CEMSA ?' clone. The cultivars with higher resistance to black Sigatoka showed a tendency to decreased oxygen uptake; this is probably due to the damaged tissues, instead of a toxicity effect of toxin treatment. At present, the first somatic embryos obtained from irradiated cellular suspensions are in the germination phase and somatic embryogenesis in genotypes from the IMTP is being developed. The introduction of foreign genes into the plant genome is a basic technique to study gene expression and physiological processes in plants and for breeding programmes.Improving the agronomic value of major crops is likely to involve the introduction of multiple genes, many of which will not provide directly screenable phenotypes among the initial products of transformation. Major restrictions of current transformation techniques are that only a few genes can be transferred at the same time and that selectable marker genes have to be used, which frequently results in transgenic plants containing undesirable antibiotic resistance genes.The objective of the present study is to determine the efficiency of cotransformation with visually scorable marker genes using Agrobacterium tumefaciens and banana (Musa spp.). Cell suspension culture of four cultivars were infected with two different A. tumefaciens strains each carrying a distinct disarmed T-DNA containing one of three reporter genes [Luciferase (LUC), ß-Glucuronidase (GUS) or Green Fluorescent Protein (GFP)] as well as the neo selectable marker gene. Multicellular structures expressing multiple genes were recovered, and cotransformation frequencies were measured. The cotransformation frequency was less than the sum of the frequency of each single transformation. Negative correlation was found between the transient expression of two visual marker genes introduced together for cotransformation. Significant differences in (co-)transformation frequency were detected between the banana cultivars tested. We anticipate that the simultaneous use of multiple reporter genes will provide a convenient method for the accurate determination of cotransformation and will contribute to a strategy for multigene transformation.A. Elsen and D. De Waele Laboratory of Tropical Crop Improvement, KULeuven, Kasteelpark Arenberg 13, B-3001 Leuven, Belgium Among nematodes parasitizing bananas throughout the world, Radopholus similis and Pratylenchus coffeae are important migratory nematodes, causing severe yield losses in commercial and local consumption cultivars. Chemical control is currently the most used method to manage the nematodes although nematicides are dangerous, toxic, and expensive. Therefore, nematode control through genetic improvement of banana is widely encouraged. Many Musa cultivars have been screened to find resistance against these root pathogens. Screening research is time consuming because it most be carried out both under field and greenhouse conditions. In vitro screening could facilitate and hasten incorporation of genetic nematode control into bananas. However, an in vitro screening method requires aseptic nematode cultures.In this paper, the development of aseptic cultures of R. similis and P. coffeae and an in vitro screening method are discussed. Alfalfa callus on modified White's medium has proved to be a good aseptic culture system for both R. similis and P. coffeae. Although the reproduction is significantly lower compared to carrot disc cultures, this system has many advantages. The nematodes are not only cultured under complete aseptic conditions but this system is also less labour intensive and offers a more continuous inoculum production.In addition, culturing on alfalfa callus did not alter the pathogenicity of R. similis and P. coffeae. Both R. similis and P. coffeae could infect and reproduce on the roots of in vitro grown 'Grande naine' plants. For both nematodes necrotic lesions were observed in the roots within 2-3 weeks after inoculation. In a last experiment, the reproduction of R. similis was tested in vitro on six different Musa cultivars with a known host response to R. similis. Except for 'Yangambi km5', their host response under in vitro conditions corresponded to their host response under greenhouse or field conditions. The susceptible status of 'Grande naine', 'Gros Michel' and 'Cachaco' was confirmed as well as the resistant status of 'Pisang jari buaya' and 'SH-3142'. The effect of lectins on the plant parasitic nematode Radopholus similis was studied in a series of experiments. FITC-or colloidal goldlabelled lectins of Canavalia ensiformis (ConA), wheat (WGA) and Helix pomatia (UPA) were found to bind the nematode in the head region, at the excretion pore, the pores of the reproduction system and those of the phasmids. The viability and the chemotactic response towards plant roots, after treatment of nematodes with lectins, were examined in vitro by analyzing movement tracks left on agar plates. The assay included six plant lectins of five different classes and the banana thaumatin-like protein. A 1% concentration of Phaseolus vulgaris agglutinin (PHA) had a toxic effect on R. similis females: 68% showed no or very little movement after inoculation compared to an average of 30% for other lectins and 5% for the control treatment. A 0.05% concentration of PHA still reduced the viability of R. similis females by 75%. ConA and WGA did not alter the chemotactic response towards plant roots, despite of the demonstrated binding of both lectins to R. similis. In contrast, Galanthus nivalis agglutinin (GNA) reduced orientated movement of R.similis females towards plant roots. Finally, the secretions of R. similis were stained with Coomassie Brilliant Blue R. These secretions appear at the amphids, the excretion pore, the vulva, the spicules and the phasmids. Moreover, nematodes treated with GNA produced less abundant secretions. Differential AFLP patterns were obtained and up to 25% polymorphism was observed depending on the primer combination and the cultivar. TE-AFLP analysis generated shorter and a lower number of fragments resulting in only relatively few polymorphisms between the dwarf and normal-sized cultivars. The somaclonal variants obtained in vitro from the dwarf 'Curare enano' might have been caused by methylation induced by in vitro conditions. MSAP analysis, based on the methylation (in)sensitivity of a pair of isoschisomeric restriction enzymes, appeared to be a valuable tool in revealing differential cytosine methylation. Cloning and sequencing differential fragments did not reveal significantly homologous matches in public databases. cDNA-AFLP analysis between the dwarf and normal 'Curare enano', revealed a normal-specific fragment, while cDNA-TE-AFLP analysis resulted in a dwarf-specific fragment. AFLP and the variant techniques have shown the potential to differentiate between closely related genotypes. More primer combinations and/or alteration of restric-tion enzymes will increase the chance of finding more dwarf-related sequences. Banana (Musa spp.) is an important and major fruit crop in India, which is the largest producer of bananas in the world. However, diseases and pests such as black Sigatoka and Panama disease, bunchy top virus and nematodes remain as major threats to production. Genetic modification using embryogenic cell suspensions (ECS) appears to be a suitable approach for integrated genetic improvement. Progress has been made in the development of protocols for the establishment of ECS, and immature male flowers as well as proliferating in vitro cultures have mainly been used.Especially in vitro proliferating meristems ('scalps') are an ideal starting material, because they can be generated all round the year from most cultivars. The method includes a preculture of the proliferating meristems on high cytokinin medium which provides embryogenic competence. Several important Indian cultivars [('Robusta' (AAA), 'Basrai' (AAA), 'Shrimanthi' (AAA), 'Karpoora valli' (ABB)] have been employed in this study for the induction of good quality scalps and embryogenic callus. Of these, 'Robusta' have shown the formation of embryogenic callus, which subsequently has been used for the establishment of ECS. In addition, the effect of alternative cytokinins such as meta-topoline (MT, thidiazuron (TDZ) and N-chloro-4-pyridyl-N'-phenylurea (CPPU) has been studied on isolated meristems of 'Cachaco' (ABB), 'Williams' (AAA) as well as on scalps of 'Robusta'. On isolated meristems ('Williams', 'Cachaco'), CPPU has only resulted in the development of single shoots and roots, whereas MT resulted in the formation of watery callus and proliferation. TDZ mainly induced swelling of the explants. TDZ proved to be better cytokinin over MT in the induction and maintenance of good quality scalps. These are currently under evaluation for embryogenic induction. The established ECS will be characterized and used in cryopreservation and genetic transformation experiments.Philippine banana cultivars This report summarizes the results of our efforts to evaluate the usefulness of DNA marker techniques, such as random amplified polymorphic DNA (RAPD), microsatellites or simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP), to characterize the genomic alterations in induced mutants of the two Philippine banana cultivars. Mutants were induced in the two most popular Philippine edible banana cultivars by gamma and fast neutron irradiation from in vitro shoot-tip cultures. Promising clones were selected and evaluated further using molecular markers. In banana, several DNA marker techniques were used to investigate genetic relationships between Musa accessions and to determine differences in somaclonal variants and radiation induced mutants. In this study, RAPD, SSR, and AFLP techniques were successfully used under local conditions and found useful in characterizing irradiated and non-irradiated banana clones. RAPD, SSR and AFLP markers also showed sufficient polymorphism to differentiate between the two cultivars used. However, SSR and AFLP markers were found to be more highly reproducible. One of the most significant accomplishments of this CRP project is the development of a high quality, nonradioactive, silver-staining technique for AFLP analysis, which can be easily adopted under laboratory conditions in developing countries. AFLP markers showed both high reproducibility and discriminating capacity. Polymorphic AFLP markers were identified (Table 2) and found useful in fingerprinting bananas and other Musa species. AFLP was the only marker technique tested, which was able to detect variation in DNA profiles of induced mutant clones, their first cycle suckers and non-irradiated control clones (Figure 2), which ELISA assays on extracts from lyophilized banana pulp showed that the concentration of the Ace-AMP1 peptide reached up to 0.0316% of the total amount of soluble proteins or six times above the background signal measured in non-transformed banana pulp. We tested whether this expression level had an effect on rats fed on a diet containing transgenic banana pulp. While energetic content was comparable in transgenic and control pulp, dry matter and protein content were lower and higher in transgenic pulp than in control pulp respectively. Twenty per cent of lyophilized meal from control or transgenic bananas were incorporated in regular rodent food and supplied to male and female Wistar rats. Feeding of the transgenic meal during six weeks did not cause any difference in food intake, growth rate and weight of internal organs in comparison to feeding on control diet. Also, a complex blood analysis did not show any effect in rats consuming the transgenic banana meal. Then, the putative virus-free material was tested again after greenhouse acclimatization. The virus eradication rates after cryopreservation for CMV and BSV reached 30% and 90% respectively. In comparison, the frequency of virus-free plants regenerated directly from highly proliferating meristems, which reflects spontaneous eradication, reached 0% and 52% for CMV and BSV respectively. The conventional meristem culture resulted in 0% CMV-free plants and 76% BSV-free plants.In conclusion, cryopreservation seems to be a very promising technique for virus eradication from Musa germplasm enabling to faster distribute germplasm of interest. The most sensitive reporter system, bioluminescent luciferase (LUC) is used in T-DNA mediated promoter tagging. Since integration of the promoterless luc gene is random, the level of LUC expression is suboptimal in most transformants to be screened requiring a highly sensitive detection. Preliminary results on in vivo screening of LUC expression in hundreds of putative promoter tagged cell cultures will be presented. Chemiluminescence has been for several years the method of choice in our laboratory for non-radioactive hybridization analysis.Although exposure to and development of an X-ray film is a sensitive technique, it is time consuming and costly because multiple exposures are needed for the evaluation of results.In addition to increased flexibility and higher sensitivity of detection, signals can be captured faster with the CCD camera than with film. Good results can be obtained with a single exposure by adjusting the gray scale of the captured image as will be demonstrated.In addition to luminescence the camera can also detect fluorescent signals, which is demonstrated by the ability to monitor green fluorescent protein expression in transgenic banana cultures. Quantification of light intensity by software analysis will be demonstrated. Genetic transformation of banana (Musa spp.) by particle bombardment and Agrobacterium is established only in a few laboratories worldwide. In general, transformation frequencies are reported to be cultivar dependent. Thus, there is a need to optimize established transformation protocols for any particular type of banana. In this study, the two transformation methods were compared and the effect of physical parameters on transformation frequency was investigated in four banana cultivars: 'Grande naine' (AAA), 'Obino l'ewai' (AAB), 'Orishele' (AAB), and 'Three hand planty' (AAB). DNA transfer frequency was measured by monitoring expression of the b?glucuronidase and the green fluorescent protein gene. The results indicate major differences between the two transformation systems. Significantly higher transient and stable gene expression, in all banana cultivars, were obtained with the Agrobacteriumbased method. The effects of age and volume of cell suspensions as well as the length of infection were optimized. The cultivars were categorized on the basis of their competence for transformation and their capacity for regen-eration. Molecular and biochemical analysis will be performed to confirm integration and expression of the transgenes in the different cultivars. The initiation of embryogenic cell suspension cultures of banana is still difficult and timeconsuming, irrespective of the starting material used (immature male flowers, immature zygotic embryos or proliferating in vitro meristems). The embryogenic response is very low and slow. Indeed for most cultivars less than 1% of initial explants give rise to an embryogenic callus suitable for cell suspension initiation and 9-26 months are needed before such embryogenic cell suspension is established. Moreover, once established, these cell suspensions are subject to somaclonal variation and microbial contamination and a prolonged culture period may result in a lower and eventually a total loss of morphogenic capacity.Up to now, transformation protocols of banana rely on embryogenic cell suspensions. Particle bombardment as well as Agrobactenum-based protocols resulted in transgenic banana plants. Somatic hybridization and protoplast electroporation are also both depending on the isolation of regenerable protoplasts from embryogenic cell suspensions. Finally, embryogenic cell suspensions can be used for mass propagation as an alternative to shoot-tip cultures.The safe preservation of this valuable suspensions through cryopreservation is thus of outermost importance. A cryopreservation technique was developed which involves cryoprotection with 7.5% DMSO (dimethyl sulphoxide) and 180 g/L sucrose, followed by slow freezing at 1°C/min to -40°C and plunging into liquid nitrogen. Currently, 651 cryotubes containing embryogenic cell suspensions belonging to 48 independent cell lines and 14 different cultivars are safely stored in liquid nitrogen for the long term. Recently, banana cell suspensions were recovered after five years storage in liquid nitrogen. The ability to produce somatic embryos remained intact. Also their competence towards Agrobacterium-mediated transformation was screened and compared to a noncryopreserved cell suspension of the same cell line. The transient expression of the introduced marker gene as well as the regeneration efficiency of transgenic plantlets was comparable. The mechanisms of action of M. fijiensis toxins in black leaf streak (BLS) disease were studied. The ethyl acetate crude extract (EaCE) from the pathogen culture filtrates and juglone (5-hydroxy-1,4-naphthoquinone), which is a purified metabolite from EaCE, were injected in the leaves of two banana cultivars. The cultivars 'Grande naine' and 'Fougamou' served as a susceptible reference and partially resistant reference respectively. These bioassays induced necrosis and showed decrease of the vitality index determined according to chlorophyll fluorescence data (Lichtenthaler et al. 1986). The 'Grande naine' cultivar was more sensitive than Fougamou whatever the bioassay (induction of necrosis or chlorophyll fluorescence) taken into account. The lightdependence of the toxicity revealed by these tests, the early effect on chlorophyll fluorescence (Harelimana et al. 1997) and the swelling of 'Grande naine' chloroplasts after injection with EaCE, are indicative that chloroplasts could be a potential target site for M. fijiensis toxins.A mechanical protocol (Leegood and Malkin 1986) to isolate physiologically intact chloroplasts from banana leaves was developed. A new bioassay based on the addition of juglone to banana chloroplast suspensions was used to analyse the impact of M. fijiensis metabolites. By performing the Hill reaction (Allen and Holmes 1986) with the so treated suspension to measure the ability of chloroplasts to transfer electrons, a direct inhibiting effect of juglone on this physiological activity was clearly demonstrated. Moreover, this effect was again higher with 'Grande naine' chloroplasts than with those of 'Fougamou'. Since chloroplasts constitute one of the sites of active oxygen species production in plants (Sutherland 1991, Foyer et al. 1997), their direct interactions with juglone in bananas led to a new hypothesis. Hence, oxidative events were suspected to be at the origin of the physiological damages in the isolated chloroplasts.In fact, involvement of fungal naphthoquinone metabolites in oxidative process in not uncom-mon (Medentsev and Akimento 1998). Their auto-oxidative property responsible of the oxidation of NADH and NADPH leads to the removal of these molecules from the oxidative phosphorylation system as potential sources of reduction equivalents for the respiratory chain.In the case of BLS disease, assessment of this hypothesis was performed by considering possible interactions between juglone and banana antioxidant systems. We observed that juglone causes an in vitro oxidation of ascorbic acid, the most abundant antioxidant in plants (Smirnoff 2000). The occurrence of oxidative phenomena induced by this metabolite in bananas was also assessed by analysing the superoxide dismutases (SOD) patterns at several intervals of time following juglone injection into leaves of the two reference cultivars. In fact, superoxide dismutases are assumed to play a central role in the defence against oxidative stress (Beyer et al. 1991, Scandalias 1993). Our preliminary observations showed that there was a repressive effect on one SOD isoform in 'Grande naine' while a stimulating effect on another SOD isoform in 'Fougamou' seemed to occur. On the base of the results obtained with the two antioxidant systems analysed previously, juglone could be supposed to deprive bananas partly of their antioxidant capacity.Further investigations have to be done in this area in order to determine exactly all the mechanisms affected by the pathogen metabolites during BLS development.Finally, a first in vitro selection assay for BLS resistance was also performed. Therefore, different juglone concentrations were mixed with embryogenic cell suspensions of two lines of THP ('Three hand planty' cultivar) as well as with suspensions containing somatic embryos of the same cell lines. After an overnight incubation, the material was transferred onto juglone-free fresh media. In general, both plant materials necrosed and did not show any weight increase during the period of incubation following the treatment. However, from somatic embryos of one treated line, some plants were obtained from tissues that did not become necrotic with 50 ppm of juglone. These regenerated plants are going to be evaluated for their eventual BLS resistance with the abovementioned bioassays as well as by artificial inoculation of the pathogen. These plant regenerates will constitute a precious material to further analyse the role played by M. fijiensis toxins in development of BLS disease.","tokenCount":"47643"} \ No newline at end of file diff --git a/data/part_6/0845647738.json b/data/part_6/0845647738.json new file mode 100644 index 0000000000000000000000000000000000000000..f5aeb1af616de2efe466b328c9db0124a3b310ed --- /dev/null +++ b/data/part_6/0845647738.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"62f033f4c6d7c51e6d731f23c9520984","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c196e2ef-5583-4297-8ae3-6465c05b8a18/retrieve","id":"-1751694123"},"keywords":[],"sieverID":"41330420-96f2-4f02-a154-7ff780669482","pagecount":"11","content":"This study applied logit and transformed logit regression to examine factors affecting the adoption of orange flesh sweet potatoes, and intensity of such adoption, by a representative sample of 340 farmers in the Busia and Rachuonyo (OFSP) districts of Kenya in 2009. A double-censored Tobit model was also used to study factors affecting intensity of adoption. The study also investigated whether participation in a value chain extension intervention programme increased farmers' likelihood of adopting OFSP. The participation variable was first tested for endogeneity and \"purified\" before using it as a \"proxy\" in the adoption regression. The results suggest that the district where the farmer comes from, knowledge on value addition and nutritional benefits, and availability of vines were the key factors for adoption. The results also suggest that participation in a value chain extension programme enhanced the probability of adoption. Factors affecting intensity of adoption were site, value addition, vines availability, level of commercialization and having a child of up to five years.Sweet potato (Ipomea batata L.) is an important traditional crop that is grown customarily by small-scale farmers in many developing countries mainly for household consumption. It is traditionally regarded as a \"poor man\"s\" crop as it is typically grown and consumed by resource poor households, and mainly by women, and it gives satisfactory yields under adverse climatic and soil conditions, as well as under low or non-use of external inputs (Carey et al., 1999;Kung\"u, 1999;Ndolo et al., 2001;Githunguri and Migwa, 2004).As a food security crop, it can be harvested piecemeal as needed, thus offering a flexible source of food and income to rural households that are mostly vulnerable to crop failure and consequently fluctuating cash income. In addition to being drought tolerant and having a wide ecological adaptation, it has a short maturity period of three to five months. It is also an excellent source of vitamin A, especially the orange fleshed varieties (Ndolo et al., 2001). The orange fleshed varieties are also tasty and have attractive color to children (Kaguongo et al., 2008a) hence have high potential to address caloric and vitamin A deficiency problems of children among the poorest communities (Stathers et al., 2005;van Jaarsveld et al., 2006;Low et al., 2007). However, most varieties in sub-Saharan Africa are white-fleshed, low yielding and lacking beta-carotene, the precursor of vitamin A (Stathers et al., 2005).Sweet potato is produced in most parts of Kenya, being concentrated in districts of Nyanza and Western provinces. About 60% of the households in these two provinces live below the poverty line (Central Bureau of Statistics (CBS), 2003), an indication of a potentially high proportion of the population without adequate quantity and quality of food intake. The potential of sweet potato\"s contribution to food security, increased incomes and reduction of nutritional deficit is, therefore, considerable and is yet to be fully exploited in developing countries (Woolfe, 1992).Although commercialization of sweet potato is still low in most parts of Nyanza and Western provinces, its gross margin of USD 238 to 362 ha -1 is highly competitive when compared with that of maize (USD 55 to 244 ha -1) and cassava (USD 97 to 171 ha -1) which are the other important crops commonly grown in these regions (Nyoro, 2002;Stathers et al., 2005;Kaguongo et al., 2008b;Fermont et al., 2010). However, the area allocated to sweet potato is often a small fraction of area allocated to maize or cassava because sweet potato is often grown as a subsistent crop (Gakonyo, 1993;Kaguongo et al., 2008b;Fermont et al., 2010).The Traditional Food Project was a programme in Kenya and Tanzania jointly implemented between April 2007 and September 2009 by the International Potato Center (CIP), Farm Concern International (FCI), Urban Harvest (UH), and World Vegetable Center (AVDRC-Asian Vegetable Development Center). Its aim was to increase productivity, utilization and marketing of Traditional African Vegetables (TAVs) and sweet potatoes, specifically orange flesh sweet potatoes (OFSP). The project aimed to achieve this through the delivery of improved extension services to the farmers participating in the programme. The three OFSP varieties promoted in Busia and Rachuonyo districts in Kenya were Ejumula, Vindolotamu and Vitamu-A.To promote increased adoption, commercialization and marketing of improved varieties of the targeted crops by the programme farmers, the project used a \"Commercial Villages\" (CV) approach. The approach uses a collective approach in interventions aimed at increasing adoption, productivity and commercialization by the rural poor. In the scheme, farmer groups are clustered together to form one large group called a \"commercial village\" that aims to benefit from economies of scale in extension work, input sourcing, production and marketing activities.To evaluate the impact of interventions from this programme, the researchers plan to conduct impact analyses using baseline and adoption data generated from participants (members of the programme) and nonparticipants (non-members of the programme). However, before undertaking any impact assessment, it is imperative to establish whether the programme participation by farmers was instrumental in the adoption of improved technologies and innovations and control for confounding factors that affect the adoption and impacts measured in terms of any outcome variable. The objective of this study is, therefore, to analyze the adoption of OFSP among sample farmers in two provinces of Kenya by identifying key determinants of adoption and intensity of adoption of OFSP, and establishing whether programme participation enhances adoption and intensity of adoption. The review of adoption studies by Feder et al. (1985) indicated, inter alia, that adoption decisions are influenced by a number of socioeconomic, demographic, ecological and institutional factors and are dependent on the nature of the technology. Studies of the key determinants of technology adoption by farmers growing upland rice and soybeans in Central-West Brazil (Strauss et al., 1991) and to evaluate the role of human capital and other factors in adoption of reduced tillage technology in corn production (Rahm and Huffman, 1984) found that farmers\" education and experience play a crucial role in facilitating technology adoption. Doss (2003) reported that the major reasons for not adopting farm-level technology in East Africa were: (1) farmers\" lack of awareness of the improved technologies or a lack of information regarding potential benefits accruing from them; (2) the unavailability of improved technologies; and(3) unprofitable technologies, given the farmer\"s agroecological conditions and the complex set of constraints faced by farmers in allocating land and labor resources across farm and off-farm activities. The mismatch between technology characteristics and farmers\" technology preferences has also been identified as the most important factor for the low level of technology adoption in Ethiopia (Wale and Yallew, 2007).Other studies have revealed that off-farm incomes and availability of information influence technology adoption decisions through affecting risk aversion levels of smallholder farmers. Risk aversion level is likely to be negatively associated with adoption as farmers are less certain about the profitability (productivity) of new technologies when they use them for the first time. Farmers level of risk aversion (which is a function of their poverty level, lack of information on the productivity of the technology, and non-stability of the impact of the technology) is also an important factor in the adoption decision (Feder and Slade, 1984;Feder et al., 1985;Kristjanson, 1987;Kaguongo et al., 1997).To improve availability of relevant information for increasing adoption, many development agents have devised several approaches and innovations. When the innovation system is linked to farmers to promote effective communication, problem identification, problem solving and personal interactions of a formal or informal nature, higher adoption of technology is likely (Steffey, 1995). Putler and Zilberman (1988) revealed the importance of physical capital endowment in the adoption process. Physical capital commonly associated with adoption of technologies has been identified as farm size or cultivated land, livestock and farm implements owned (Feder and O\"Mara, 1981;Rahm and Huffman, 1984;Shapiro, 1990;Nkonya et al., 1997). Financial capital and credit access have also been shown to affect adoption of agricultural technologies and innovations especially when such adoption does not involve increasing diversification, which is viewed as a reducing measure (Feder and Umali, 1993;Cornejo and McBride, 2002;Simtowe and Manfred, 2006).A Kenyan study, which evaluated the effect of women farmers\" adoption of OFSP in raising Vitamin A intake, found that women farmers were likely to adopt the OFSP if the clones were sufficiently high in starch (high dry matter), low in fibre, and if they were introduced through community-level education programmes that focused on the health of young children (Hagenimana and Oyunga, 1999). A study in Mozambique revealed that some of the key factors affecting adoption of OFSP included availability of vines, intensity of extension service and number of times the respondent received vines (Mazuze, 2005). A number of studies have also revealed that most of the factors affecting adoption also affect the intensity of adoption (Alene et al., 2000;Kaliba et al., 2000).The study was conducted in Rachuonyo (Nyanza province) and Busia (Western province) districts of Kenya. The Rachuonyo site comprised of the most commercialized sweet potato area in the country. Nyathiodiewo, a local variety which is yellow fleshed, is the most commonly grown variety accounting for over 90% of total production in the area. Traders from major towns of the country (Nairobi, Kisumu, and Nakuru) bought sweet potatoes from the district and transported them using large trucks. Sweet potato is also regarded as a food security crop in the area and is particularly important when there is an undersupply of maize. The site is located in the lower midland tea zone (LM2), with elevation ranging from 1,300 to 1,700 m and mean annual precipitation of 1,300 to 1,700 mm. The long rains occur from February to June while short rains occur from August to November.The Busia site comprised of an area where sweet potato is less commercialized although sweet potato is important as a food security crop and farmers produce it on a small scale mainly for home use and only sell when there is an excess or when there is a pressing demand for cash. The area falls within the marginal sugarcane zone (LM1), with elevation ranging from 1,200 to 1,300 m and annual precipitation of 1,400 to 1,550 mm. The mean annual temperature ranges from 20.4 to 22.3°C. Sweet potato is planted in the months of April through June during the long rains and September through mid-November during the short rains. Sweet potato varieties grown are mainly white fleshed such as Bungoma and Kampala, and none is predominant in the area.Farmers were grouped into participants if they participated in the Kaguongo et al. 495 traditional Food Program and non-participants if they did not.Baseline and adoption survey data were collected for the purpose of undertaking impact assessments by comparing both participant and non-participant farmers before and after the programme implementation. The baseline survey was conducted in September to October 2007 while the adoption survey was conducted in November to December 2009. The two surveys used structured questionnaires to gather information on socio-economic characteristics, cultivation, consumption and marketing of sweet potatoes by the households. Each site had four CVs comprising of about seven farmer groups each and with an average of 18 participants per group. Representatives of participant farmers (beneficiaries) and nonparticipant farmers (non-beneficiaries) were interviewed during the baseline and adoption surveys. During the baseline survey four farmers were randomly sampled per group per CV. The same farmers in the baseline survey were targeted during the adoption survey, but due to high attrition additional participants were sampled for the adoption survey. The non-participant farmers who acted as the \"control\" for the study were sampled from villages with similar characteristics as those from which participant farmers in CVs originated. Twenty non-participant farmers were randomly sampled to act as control for each CV. A total of 340 farmers were interviewed during the adoption survey, of which 205 were participants and 135 were non-participants. Due to the nature of the programme interventions, the sample selection criteria included two priorities: the first priority was targeting farmers who were members of farmer groups and the second priority involved targeting farmers with children of up to five years during the onset of programme implementation (2007). However, lack of foreseeable benefits by would-be \"control\" farmers resulted in non-willingness to participate in the study, hence the priority one was relaxed for \"control\" farmers. Relaxation of priority one and retaining priority two resulted in more \"control\" farmers having children of up to five years and fewer of them belonging to farmers groups compared to nonparticipant farmers.Data collected were entered and \"cleaned\" using CSPro. The SPSS software package (Norušis, 2005) was used for data processing while the STATA package (StataCorp, 2008) was used for econometric analysis.Modeling farmers\" decision making about whether to adopt or not to adopt a technology constitutes a discrete (whether or not to take up the technology) and continuous (the intensity of use of the technology) decision (Wale and Yallew, 2007). Most adoption models are based on the assumption that farmers are faced with a choice between two alternatives and the choices they make depend on identifiable characteristics of the technologies (Pindyck and Rubinfeld, 1997).In a logit model, the parameter estimates are linear and, assuming a normally distributed disturbance term (μ), the logit maximum likelihood (LML) estimation procedure is used to identify explanatory variables affecting the adoption of OFSPs.According to the logit model, the likelihood of an individual farmer adopting a new technology t2, given a well-defined set of socioeconomic and physical characteristics (X), is represented as:(1)Where: Pi = is likelihood of the i th farmer adopting a technology, labeled as Adoptioni; Xi = exogenous variables; â = the coefficients. The adoption logit model was specified as:Table 1. Summary description of the variables used in modeling adoption and intensity of adoption. The wealth index (WID) was calculated from cultivated area of land, total livestock units and number of equipment items and tools owned by the farmer using principal component analysis (PCA). Attempts to use individual assets in regression models result in an unnecessarily high number of explanatory variables and create multicollinearity. Use of PCA is convenient since it solves the problem of aggregating assets of different units and controls multicollinearity which is likely to occur when many types of asset variables are included in the regression equation (Nieuwoudt, 1977;Filmer and Pritchett, 2001;McKenzie, 2003;Vyas and Kumaranayake, 2006). Table 1 shows summary details of variables in the econometric models used in this study.Since participation in extension programme was one of the factors evaluated for its influence on the adoption of OFSP, there was a need to test for endogeneity. Selection bias occurs because participation is rarely random and this is often correlated with the outcome variable of interest (Heckman, 1979;Goodfellow et al., 1988;York, 1998;Cuddeback et al., 2004). A Hausman specification test (Hausman, 1976) was performed to evaluate if the participation variable (Pi) was endogenous.The exogeneity of Pi was tested using the estimated residual ( i) from the reduced form equation (participation regressed on its instruments) as explanatory variables in the structural equations (with adoption as the dependent variable).(2)Where Yi is a vector of variables postulated to affect participation and Wi is a vector of exogenous variables postulated to affect adoption of OFSP.If the residual variable of the reduced form Equation ( 2) is correlated with the dependent variable in the structural Equation (3) of adoption, then it means the participation variable is endogenous (Gujarati and Sangeetha, 2009). To get rid of the correlation between the Pi and a Heckman\"s two-stage regression approach was used where the participation variable (Pi) was first regressed on all the predetermined variables in the whole system, involving participation Equation ( 2) and adoption Equation (3) (Zuehlke and Zeman, 1991;Arendt and Holm, 2006;Kacagil and Demir, 2006).The predicted i estimated from the instrumental equation, which is free from influence of the stochastic disturbance ì, is used in the second stage regression replacing Pi.The intensity of adoption in this paper is defined as the proportion of area under OFSP and is estimated as a fraction of total area under sweet potatoes. The larger the proportion the more intensive is the adoption of OFSP. Wale (2010) used logit transformation regression to explain land share allocated to local coffee varieties in Ethiopia which was the response variable. In the present study, the (4)For proportion data with 0, 1 extremes and continuous values inbetween, use of OLS regression is inappropriate because predictions are likely to go beyond the 0 to 1 range. Papke and Woodridge (1996) indicate that the drawbacks of linear models for fractional data are analogous to the drawbacks of the linear probability model for binary data. Logit transformation is performed on the dependent variable as shown in Equation 5 (Birkhaeuser et al., 1991;Grigoriou et al., 2005;Wale, 2010):However, this procedure cannot be applied directly if the dependent variable takes the extreme values of 0 and 1, that is, the transformed variable cannot be evaluated. Hence, to deal with this problem the extreme values (0 and 1) are substituted with close approximations (Birkhaeuser et al., 1991;Grigoriou et al., 2005;Pryce and Mason, 2006;Wale, 2010).There were 263 (77.4%) zeros and 8 (20.4%) ones which were replaced with 0.000001 and 0.999999, respectively. After this OLS regression is conducted on the transformed dependent variable (Equation 6): In all sites, 38.2% of respondents had adopted growing of OFSP, with 66.1% of 168 households and 11.0% of 172 households adopting in Busia and Rachuonyo districts, respectively. The mean age of household head was 47.3 years and 34.1% of household heads were female. There was no statistically significant difference between adopters and non-adopters by age or gender. Households with off-farm income were also not statistically different between adopters (63.8%) and nonadopters (69.0%). More adopters (33.1%) than nonadopters (10.0%) were doing value addition of sweet potato and also more adopters (48.5%) than nonadopters (19.5%) knew that OFSP contain vitamin A (beta carotene).The number of years of formal education for adopters (7.6 years) was significantly higher than that of nonadopters (6.7 years) indicating possible positive association between education and adoption of OFSP.Testing of endogeneity started with a search for instrumental variables that influenced participation in the programme but with no direct effect on adoption of OFSP. This evaluation indicated that gender of the household head, belonging to a farmer group, total area allocated to all types of sweet potatoes, number of children of up to five years of age and area under cassava were good proxies for participation. Table 2 shows the details of the instrumental variables used to predict participation in the extension programme.The programme targeted farmer groups as a way of increasing effectiveness of extension and ensuring collective action was easily achieved, and this may explain why belonging to a farmer group highly increased the odds of participating in the programme. The coefficient for gender of the household head was also significant (p < 0.05) indicating that female headed households were more likely to participate in the programme than male headed households (Table 2).Having children of up to five years had a negative and statistically significant coefficient. The results indicate that households with more children were less likely to participate in the intervention programme possibly because child care and attending programme trainings were competing for the available time. Although area allocated to sweet potato and cassava positively affected participation in the programme as expected and improved the model fit, their coefficients were not statistically significant at the 5% level of significance. The endogeneity test using residuals from the participation instrumental equation yielded a p-value of 0.064 indicating that the Null hypothesis of exogeneity would be rejected at the 10% level of significance but accepted at the 5% level of significance. Hence, to yield efficient and consistent estimates we treated farmers\" decisions to participate in the programme as endogenous in adoption decision and followed a two-stage procedure to solve the endogeneity problem. The predicted participation variable (PRE_2) obtained from the first stage regression using all predetermined variables in all the systems was used in the adoption equation as a \"proxy\" for participation.The value from the Hosmer and Lemeshow Chi-square test was non-significant (0.108), which indicates that the binary logit model adequately fitted the data while Omnibus tests of model coefficients indicated that all predictors jointly predicted the dependent variable well at the 5% level of significance. The classification table also showed good prediction performance of 85.1% (90.5% of ones and 78.2% of zeros correctly predicted), which compared well with the Count R 2 of 89.5%. Some variables hypothesized earlier to explain adoption of OFSP were dropped from the model either because including them in the regression analysis reduced the goodness of fit of the model and their estimated coefficients were not statistically significant at the 5% level, or they were correlated with some of the factors that improved the goodness of fit. The variables dropped were such as the number of children in the household, number of years of formal education of the head, number of days in a week the household consumed sweet potatoes, and selling any type of sweet potato. Table 3 presents the estimated logit regression model results.The results indicated that site, participation in the programme, skills of value addition, knowledge about vitamin A and availability of vines were crucial factors determining adoption of OFSP. The results also suggested that age of the household head and asset ownership influenced adoption although their coefficients were only significant at the 15% level of significance. However, labor availability did not affect adoption possibly because there was no difference in labor requirement between OFSP and other varieties grown by the sample farmers.Some of the identified variables mirrored the findings from Mazuze (2005), who observed that adoption of OFSP varieties is affected by the district where the respondent resides, quality of extension and availability of vines to farmers. The study further observed that to spur adoption of OFSP, it was important to identify market opportunities for processed products and link farmers to potential processors and market outlets.According to the results, being in Busia district increased the odds of adopting OFSP than being in Rachuonyo. A farmer in Busia is 54 times more likely to adopt. This could be due to several underlying factors, which included the fact that sweet potato was more commercialized in Rachuonyo District than in Busia District and the yields of the local varieties grown in Rachuonyo were comparable to the yields of OFSP varieties being introduced. More importantly, the short time of programme implementation might not have had sufficient effect on traders\" preferences that might not have been willing to trade in the less familiar OFSP in Rachuonyo Districts. Increasing promotion campaigns targeting traders and consumers might have increased the probability of farmers in Rachuonyo adopting OFSP.The estimated coefficient for the participation variable was statistically significant at the 5% level and had a positive sign (in reference to participants), as hypothesized. The odds for the farmers who were in the programme adopting OFSP were three times higher than those who were not. This was according to the expectation of the programme implementers and researchers, who postulated that collective action resulting from aggregating farmers in commercial villages, would make it easy and cost effective for farmers to access extension services and planting materials. Although the programme was implemented for about 2.5 years, it means that farmers participating in the programme had a higher probability of adopting OFSP. This result offered justification for impact analysis, that is, researchers could conduct a more robust econometric analysis to evaluate the intensity and impact of adoption using differences in differences (DD) as earlier planned.As hypothesized, farmers who had the know-how of processing sweet potatoes were about four times more likely to adopt OFSP than those who did not have the know-how. Some farmers processed sweet potatoes into dried chips for long storage or made sweet potato flour and mixed this with sorghum or millet flour to make porridge, or mixed sweet potato flour with wheat flour for making baked products such as cakes, bread, scones and buns. Although a few farmers sold these products in the markets and institutions such as schools the majority used them at home to improve the nutritional value and reduce the costs of making the products. The products made using OFSP varieties are tastier, nutritious and appealing to farmers and hence farmers were more likely to prefer OFSP for further value addition. This means if dissemination of value addition techniques was included in intervention programmes, the adoption rate would have even been better.The regression results suggested that farmers who had knowledge about the nutritional content of OFSP were about three times more likely to adopt OFSP than those who did not have this knowledge. This was in conformity with a priori expectation as knowledge of the nutritional value of OFSP was likely to motivate adoption of OFSP, especially for home consumption. This means any Kaguongo et al. 499 programme that includes effective training on the nutritional value of OFSP is likely to enhance its adoption.As hypothesized, the results suggest a negative impact of constraint of vines, i.e. farmers who have limitations in accessing vines are less likely to adopt OFSP. However, the odds of not adopting due to constraints of vines were not high (0.104). This could be because most farmers who were not able to preserve planting materials or get them from neighbors were linked to the seed multipliers by the programme, and this reduced the odds of not adopting.The age of the household head had a negative sign as expected. According to the results, if age of the household head increases by one year, the odds in favor of not adopting increase by 2.0%. The main reasons given for older people being less likely to adopt new technologies was that they were said to be less receptive to new ideas and were less willing to take risks. This means there may be a need to review methods of technology dissemination used in the intervention programme to ensure that they are attractive to both young and old farmers.Testing the results of the OLS regression of the transformed dependent variable for heteroscedasticity using the Breusch-Pagan-Godfrey test rejected the OLS model with homoscedasticity ( 2 tabulated = 14.07 and  2 calculated = 88.99). To remedy the heteroscedasticity, a weighted least squares regression was run using White\"s heteroscedasticity-corrected variances (Robust standard errors) using Stata (Gujarati and Sangeetha, 2009).The HC3 estimator was used for heteroscedasticity correction following the suggestion that it is the best estimator, especially in small samples (Long and Ervin, 2000).Results of the transformed logit model revealed that participating in the programme and nutritional knowledge of OFSP did not influence the intensity of adoption of OFSP. However, in addition to site, value addition and constraints of vines, having a child of up to five years and selling any type of sweet potato also significantly influenced the intensity of adoption (Table 4). Having a child of up to five years positively affects intensity of adoption at the 10% level of significance. However, this variable was not significant in the double-censored Tobit model. The results of the double-censored model were similar to those in the logit transformation regression for other variables (Table 5).Site had a positive and statistically significant coefficient in the logit transformation regression results, indicating that being in Busia had a positive effect on the intensity of adoption. The same site specific reasons affecting adoption were expected to affect intensity of adoption. However, the results indicated that participation did not influence the intensity of adoption. This means once the programme influenced farmers to adopt new varieties, other non-programme factors were more important in determining the proportion of land allocated to OFSP. Similarly, although nutritional knowledge had a positive effect in intensification, it was not statistically significant.Results suggested that having know-how of value addition had a significant positive effect on intensity of adoption. Farmers who had processing techniques were able to earn extra cash from OFSP products (chips, flour and mandazi), and hence they were willing to put a greater proportion of land under OFSP. This suggests that market access was crucial to adoption intensification as suggested by the coefficient of marketing variable which was positive and statistically significant. This means that once programme participation and other factors influenced farmers to grow OFSP, possibly for home consumption, the possibility of marketing the OFSP alongside other varieties increased the likelihood of intensifying adoption. Since OFSP was promoted for both home consumption and marketing, it emerged that those farmers who commercialized any type of sweet potato were more likely to increase the proportion of land under OFSP than those who were not.Logit transformation results also indicated that constraints of vines (planting material) affect intensity of OFSP adoption negatively and significantly. The results from the two regressions (binary logit and logit transformation) mean an intervention programme that includes training farmers on how to preserve their vines and how to source vines is more likely to increase both adoption and intensity of adoption.Although having a child of up to five years of age did not seem to affect adoption of OFSP, its estimated coefficient in the logit transformation regression was positive and statistically significant. This suggests that once the farmer has made decision to adopt OFSP (for other reasons reported in Table 4) having a child of up to five years of age affects the rate of intensification positively. This could mean that the observation by farmers of how their children devoured the OFSP after harvest and the awareness that children benefited the most from consumption of OFSP, which was one of the messages delivered by the programme, probably affected intensification positively.This study evaluated the factors affecting adoption and intensity of adoption of OFSP in Busia and Rachuonyo Districts in Kenya using adoption data collected from 340 farmers in 2009. The main objective was to determine the adoption rate of OFSP, the key factors determining adoption of OFSP and intensity of adoption, and to investigate whether participation in an extension intervention programme significantly increased the probability of adopting OFSP.The empirical results revealed four factors that are important in influencing both adoption of OFSP and the intensity of adoption. These factors include (1) district where the farmer resides, (2) know-how on value addition, (3) knowledge on nutritional value, and (4) availability of vines.The study suggests that to enhance technology adoption and its intensity, the attribute preferences of farmers and site specific factors (such as annual precipitation, soil fertility and performance of local varieties) will have to be integrated into the development of improved varieties and the extension approaches should be packaged so as to build on the value systems and preferences of both experienced and young farmers.The results suggest that yield performance of OFSP in Rachuonyo district is one of the possible areas that need to be addressed to promote adoption at the site. There is a need for intervention packages to address competitiveness of new varieties against local varieties and create awareness of the potential benefits of OFSP among the value chain players. The results also suggest that farmers who had processing (value addition) techniques and those who were linked to a OFSP processor were able to earn extra cash income; hence, had a bigger proportion of land under OFSP. This demonstrates the importance of linking agricultural technology adoption with value addition and marketing. The results underpin the importance of using a value chain intervention and a collective action approach in the framework of a commercial village, where production and marketing innovations are sought, to ensure that the adoption programme succeeds. Knowledge about the desirable features of OFSP varieties and farmers\" participation in the intervention programme also boosted adoption, confirming that adoption interventions need to create awareness, train beneficiaries and engage farmers in the implementation of a programme such as the Traditional Food programme.Availability of vines also affected both adoption and intensity of adoption of OFSP; hence an extension programme should ensure adequate access to vines either through conservation in wetland or irrigated areas or through establishment of a sustainable network of vine multipliers.The age of the household head only affected adoption of OFSP and had no effect on intensity of adoption. The negative effect of age means the promotion campaigns and extension approach should be appropriate for both the young and the aged, and the attributes of the Kaguongo et al. 501 technology need to be adapted to all ages of farmers. Two factors, (1) selling sweet-potato and (2) having a child, affected intensity of adoption and not adoption of OFSP. This implies that market access is very important if an adoption programme has to push adoption of any improved varieties to a higher level. Although adoption of improved varieties may be boosted by the knowledge of nutritional benefits of home consumption, commercialization of the varieties is important for intensification. Targeting households with young children, especially when the main concern is increased consumption of OFSP, is also likely to increase the intensity of use. This also implies that intervention programmes that create awareness of the nutritional benefits of improved varieties to children are more likely to increase adoption intensity.The programme also underscores the importance of creating awareness of nutritional value and commercialization in enhancing adoption of traditional crops commonly viewed as inferior food crops. Finally, it is recommended that the benefits and costs of the programme should be comprehensively studied, considering financial, environmental, poverty and food security dimensions, and the cost effectiveness of using a commercial village approach.","tokenCount":"5530"} \ No newline at end of file diff --git a/data/part_6/0856788548.json b/data/part_6/0856788548.json new file mode 100644 index 0000000000000000000000000000000000000000..fe24b5ce127eceda69622ee8e7fc76d0e2547e5e --- /dev/null +++ b/data/part_6/0856788548.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"97b6aed1759b3f6e210431b058f8b0e3","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c5983634-101f-4a8b-a132-cb826a079286/retrieve","id":"1866692180"},"keywords":[],"sieverID":"8665447c-b337-4798-8f38-d7f2b99e4469","pagecount":"16","content":"This publication has been prepared as an output of the CGIAR Research Initiative on AgriLAC Resiliente. Any views and opinions expressed in this publication are those of the author(s) and are not necessarily representative of or endorsed by the CGIAR System Organization.Na�onal and local mul�-stakeholder pla�orms are strengthened to become func�onal and sustainable in addressing development tradeoffs and genera�ng strategies for effec�ve food, land, and water transforma�on.CGIAR partners develop and scale innova�ons that contribute to the empowerment of women, youth, and other social groups in food, land, and water systems.Interna�onal NGOs and na�onal farmers/ trade associa�ons scale agrifood system innova�ons with the poten�al to increase the net farm income of smallholders and pastoralists in targeted areas.Producer associa�ons, AgriTech companies, government agencies, NGOs, and public extension services in two LAC countries are empowered by a digitally-enabled ecosystem to offer agro-advisory services to at least 180,000 farmers and other value chain actors to manage climate risk (CRM) more effec�vely and sustainably intensify (SI) produc�on and value chains.Na�onal and local governments in two LAC countries integrate low-emission strategies with development objec�ves at the agro-ecosystem or value chain level, with an expected impact of ~150,000 ha (2022 -2024).Public-private sector, Na�onal Agricultural Research and Extension Systems (NARES), and civil society actors across subna�onal agricultural innova�on systems in three LAC countries use InnovaHub learning, knowledge management, and evidence to understand how to accelerate on-farm uptake of socio-ecological-technological (SET) innova�ons by making them more gender-responsive, produc�on-friendly, and context-specific reaching, at least 15,000 people (2022)(2023)(2024).Public and private ins�tu�ons in three LAC countries use CGIAR science, evidence, and tools to inform and shape to Agrifood Systems (AFS) related policies, incen�ves, and ini�a�ves that are more transforma�ve, sustainable, mi�ga�on-comprehensive, and climate adapta�on-friendly (2024 -2030).End hunger for all and enable affordable healthy diets for the 3 billion people who do not currently have access to safe and nutri�ous food.Reduce by at least half the propor�on of men, women, and children of all ages living in poverty in all its dimensions according to na�onal defini�ons.Offer rewardable opportuni�es to 267 million young people who are not in employment, educa�on, or training.Implement all Na�onal adapta�on Plans (NAP) and Na�onally Determined Contribu�ons (NDC) to the Paris Agreement.Stay within planetary and regional environmental boundaries: consump�ve water use in food produc�on of less than 2500 km3 per year (with a focus on the most stressed basins), zero net deforesta�on, nitrogen applica�on of 90 Tg per year (with redistribu�on towards low-input farming systems) and increased use efficiency; and phosphorus applica�on of 10 Tg per year.Shaping nutri�on-sensi�ve socioecologicaltechnological (SET) 'best bets' to opera�onalize local AFS transi�on to climate-resilient nutri�on pathways.Inclusive digitally-enabled agro-advisories for risk management.AFS development that meets both mi�ga�on and sustainable development objec�ves.InnovaHub networks for agrifood innova�on and scaling.Science-informed policies, investments and ins�tu�ons.Section 2: Progress on science and towardsThis is a simple, linear, and static representation of a complex, nonlinear, and dynamic reality. Feedback loops and connections between this Initiative and other Initiatives' theories of change are excluded for clarity.End of Initiative outcome AA Action Area IA Impact Area SDG Sustainable Development Goal Note: A summary of Work Package progress ratings is provided in Section 3.The CGIAR Research Initiative on AgriLAC Resiliente has laid the groundwork for achieving its EOIOs in Colombia, Guatemala, Honduras, Mexico, and Peru through agricultural innovation system networks operating at local to regional scales. AgriLAC Resiliente connects with a variety of networks through the Local Technical Agroclimatic Committees (MTA in Spanish) placed at the subnational scale, which brings together local, national, and regional actors. The Initiative's InnovaHubs increased the number of actors involved in strengthening climate resilience and ecosystem services, in addition to boosting farm productivity, from 68 to 131. The Initiative generated 122 knowledge products in 2023, 23 of them in collaboration with five CGIAR Initiatives: Digital Innovation, Climate Resilience, National Policies and Strategies, Low-Emission Food Systems, and Livestock and Climate. In 2023, these contributions resulted in publication of one book and three peer-reviewed articles: (i) A review of agronomic research on the milpa, the traditional polyculture system of Mesoamerica (EOIO 1), associated with SET innovations to enhance farmers' livelihoods; (ii) The development of a farmer decision-making mind map to inform climate services in Central America (EOIO 2), enhancing climate information services for more informed decision-making; and (iii) Examination of cultural and economic barriers and opportunities for women's participation in agricultural production systems: A case study in Guatemala (EOIO 5), which will support policy makers in developing tailored interventions using science-based recommendations.AgriLAC Resiliente is making significant progress toward achieving EOIO 1 across Colombia, Guatemala, Honduras, and Mexico. This Initiative is enhancing farmers' access to climate-resilient and nutrition-sensitive technologies and validating improved varieties of maize, beans, and rice customized to local conditions for over 2,200 households and close to 8,800 people. Through collaboration, the Initiative established 17 research platforms across Guatemala (2), Honduras (2), and Mexico (13) to validate climate-resilient and nutrition-sensitive technologies. Moreover, two biodiverse plots, one involving an indigenous community, were established in northern Colombia to compare agroecological practices with conventional methods. Insights gained from both the research platforms and the biodiverse plots will better inform producers' decision-making processes and help to refine and develop more tailored technological menus, as well as the MTAs to improve recommendations' technical aspects in local agroclimatic bulletins.Capacity sharing in 1,584 individuals was increased to enhance knowledge on agricultural topics, including postharvest practices to minimize grain loss and food preparation practices to preserve the nutritional value of food prepared by local communities. Two innovations were developed in 2023 aligned with EOIO 1: a user interface of e-Agrology directed to farmers as a one-stop shop where farmers can consult localized decision support for field management and Protocols for developing Food Products from Biofortified Rice. Workshops on marketing strategies resulted in development of prototype products using cocoa and biofortified rice, while knowledge exchanged during the Latin American Agronomic Research Network symposium facilitated collaboration among researchers from the region. These efforts culminated in joint publications, including a review of agronomic research in Mesoamerica and initiatives promoting resilient agricultural practices in Mexico. Toward scaling nutrition-sensitive innovations (EOIO1), significant evidence has been generated regarding the co-innovation and scaling processes of biofortified crops. Through a comprehensive case study, relevant lessons from CGIAR's Harvest Plus program have been compiled to provide insights into the enabling factors and leverage points that drove the scaling of this innovation in a regional context.The progress towards EOIO 2 underscores AgriLAC Resiliente's commitment to empowering organizations in the region to provide digitally enabled agroadvisory services. This benefits smallscale farmers and other stakeholders in managing climate risks and promoting sustainable intensification. Notably, substantial headway has been made in enhancing ETL data processes, particularly in collaboration with pivotal partner organizations such as the Meteorological Services of Guatemala and Honduras. Our collaboration with the Digital Inclusion Initiative led to the development of an e-Agrology innovation, a tailored agronomic data collection and monitoring system for Central America. In 2023, continued training and data analysis, implemented in collaboration with Work Package 4, has proven crucial for improving and effectively using e-Agrology to generate recommendations for small-scale Guatemalan farmers.AgriLAC Resiliente is enhancing information services to support farmers, with significant strides seen in Guatemala, Honduras, and Mexico (EOIO 2). In Guatemala and Honduras, scientific advances have enabled mapping information needs and implementing usercentered design. These initiatives improve the usability of local agroclimatic bulletins and drive the design of better information products for small-scale producers in both countries. In Mexico, the Initiative conducted an outcome-harvesting study demonstrating the diverse transformations achieved through MTA. Scaling MTAs to 12 states in Mexico provided approximately 100,000 farmers with essential climate information. Furthermore, empirical evidence has been gathered concerning the facilitating factors and leverage points within the co-innovation and scaling processes of the MTA's network in LAC. This evidence is synthesized in a robust case study, offering vital insights to inform future scaling strategies aimed at further extending the reach of MTA's network (EOIO 2).Progress towards EOIO 3 focused on work with stakeholders in agrifood systems to integrate climate change mitigation within the Sustainable Development Goals (SDGs) in Colombia and Peru. Opportunities were identified for formulating public policies and supporting territorial peacebuilding, mitigating the effects of climate change, and improving living conditions in conflict-affected areas. Work has highlighted the primary causes of forest loss in Peru, including access to forests and demographic changes. The Initiative also characterized greenhouse gas emissions from cocoa and livestock value chains in Colombia's Caquetá Department. Strategies for improving cocoa chains in Colombia and the Guinean pig value chain in Junín, Peru, will be scaled up and validated at governmental levels. Innovative initiatives such as the Sustainable Cocoa Innovation Challenge in Colombia, the Perumin Inspira Challenge in Peru, and the \"EncontrAR\" knowledge platform have enhanced collaboration and skills among sector entrepreneurs, promoting climate action. An analysis of the relationship between child nutritional problems in Colombia and livestock revealed significant connections, offering opportunities to design strategies that both mitigate climate change and strengthen food security, particularly in high deforestation areas in Colombia and Peru.AgriLAC Resiliente InnovaHubs link various approaches and scales, developed over years with innovation system actors, to address sustainability and climate resilience in addition to productivity issues. In Guatemala and Honduras, InnovaHubs have enhanced farmer and local organization knowledge on topics such as agroclimatic data and soil conservation (EOIO 4). Drawing on past CGIAR successes in the region, including MTAs and climatesmart villages, and using a multisectoral hub approach, similar to one implemented in Mexico, AgriLAC Resiliente aims to adapt and bundle innovations to improve climate resilience, ecosystem services, and nutrition. Annual InnovaHub meetings in both countries foster innovation through collaborative learning and knowledge sharing, with 25 institutions in Guatemala and 14 in Honduras participating in 2023.In line with AgriLAC Resiliente´s commitment to bolstering local capacities and fostering innovation, a comprehensive training strategy has been implemented to empower stakeholders through the InnovaHubs framework (EOIO 4). Emphasizing practical application and knowledge exchange, this strategy ensures that local actors are equipped with the skills necessary to drive agricultural advances effectively. A significant initiative in Guatemala involved establishing a Digital Agricultural plot, supported by the Digital Innovation Initiative and the Centro Universitario de Oriente. Serving as a testing ground for advanced agricultural technologies (EOIO 4), this initiative directly benefits small-scale farmers in the dry corridor of Guatemala, providing them with access to cutting-edge tools and practices aimed at improving productivity and sustainability in their operations.In 2023, the Initiative spearheaded a series of studies in Guatemala aimed at unraveling insights into climate resilience, migration dynamics, and gender roles. These efforts included creating tools to support informed decision making. The public policy mapping helped identify opportunities and feasible applications of an Integrated Agrifood System Initiative (IASI) methodology, resulting in tailored public policy recommendations. This mapping underwent validation with stakeholders and significantly influenced deliberations at the Second National Forum on Migration and Climate Change, in Guatemala. The subnational mapping of key programs and interventions in food security and nutrition, along with studies on women's participation in agricultural activities and climate-induced emigration, reflects a strategic effort toward informed policy formulation and resource optimization in Guatemala.To engage in the policy science interface, we have supported the CAC in activating the Technical Group of Innovation, which gathers the NARS of eight countries. Together they have developed an innovation agenda that AgriLAC will support by enhancing their capacity to develop and scale agricultural innovations in the context of climate change. These initiatives signify progress towards EOIO 5. We also developed a case study about the co-innovation and scaling processes of climate-smart agriculture country profiles, aiming to inform the scaling of socio-institutional innovations and sciencepolicy engagement processes (EOIO 5).The MELIA team is assessing EOIO progress by conducting studies on AgriLAC Resiliente's impact. In Guatemala, they've worked with Work Package 5 to publish research on rural women's challenges and prospects in agriculture. They're measuring the Initiative's effects at three levels: 1) at the farmer level in Guatemala, where they've gathered initial data for future analysis due to budget limits, 2) at the stakeholder level, collecting baseline data from partners for a future social network impact evaluation in 2024, and 3) for meso-and macro-level outcomes, they have devised a qualitative evaluation and plan fieldwork in 2024. AgriLAC reached 2,207 farming households, benefiting some 8,800 people with more nutritious and climate-resilient biofortified seeds in Colombia, Guatemala, and Honduras.A menu of technologies was adapted to local conditions for maize, rice, and beans and validated in Colombia, Guatemala, and Honduras.A diagnostic study was made on establishing a rice processing plant to enhance productivity and quality for better access to markets-a prototype product was made with cocoa and a protocol for a prototype of food products was made with biofortified rice.EOIO 2: 180,000 farmers and agrifood system actors in two LAC countries are empowered by a digitally-enabled ecosystem to manage climate risk more effectively.AgriLAC Resiliente scaled information services in Honduras, Guatemala, and Mexico, reaching more than 100,000 farmers.ETL processes -to change how partners manage data -are in place for partners in Guatemala and Honduras.Well-established partnerships, including i.e. deploying weather stations with some partners to target blind spots for meteorological monitoring. Building from the bottom up, many technologies and capacities will underpin the Data Hub.Data analysis with an innovative Explainable Machine Learning analytics workflow enabled the generation of the first set of recommendations for small-scale Guatemalan farmers, which will be disseminated through established delivery channels and as part of the technological menu of the Innova-Hub (with Work Packages 1 and 4).AgriLAC Resiliente developed approaches to integrate climate change mitigation efforts and those aimed at delivering SDGs in Colombia and Peru.In Peru and Colombia, we identified potential to reduce greenhouse-gas (GHG) emissions from the production stage.In Colombia, sustainability strategies for cacao chains were scaled up to governmental levels and have been validated in two departments, while a roadmap for the Guinean pig value chain will be validated in Peru.AgriLAC Resiliente innovated mechanisms to share knowledge among stakeholders through a Sustainable Cocoa Innovation Challenge in Colombia and Perumin Inspira Challenge in Peru and established a knowledge platform (\"EncontrAR\").Analysis of the relationship between child nutritional problems in Colombia and livestock, to open opportunities to design strategies that simultaneously address objectives of climate-change effects mitigation and strengthened food and nutrition security, especially in areas with high deforestation rates in Colombia and Peru.Dedicated efforts fostered collaboration and innovation among stakeholders at the subnational level in Guatemala, Honduras, and Mexico.Characterization studies implemented in Guatemala and Mexico to map nutrition, market access, and technical information dissemination offered insights into Innova-Hub's impact and challenges regionally. Dedicated efforts were made to disseminate agroclimatic information through MTAs in Guatemala and Mexico and to enhance the reach and usefulness of agroclimatic bulletins.Annual Innova-Hub meetings were conducted to facilitate learnings among agrifood actors in Guatemala and Honduras.EOIO 5: Three LAC countries use CGIAR science to inform and shape agrifood system-related policies, incentives, and initiatives.Newly implemented or continued studies in Guatemala produced insights into climate resilience, migration, and the dynamics of gender roles, and also generated new tools.Public policy mapping in Guatemala on food security, climate change, and migration was finalized and validated with stakeholders and informed discussions at a national forum.Subnational mapping of key programs and interventions in food security and nutrition to identify areas with possible investment gaps and saturation of interventions in Guatemala caught the attention of a group of donors; development of an interactive platform is in process.Two quantitative studies in Guatemala (one with MELIA) led to identifying and designing additional studies for Guatemala and Peru. Set of tools and methods for exploring demand condi�ons (and associated marke�ng opportuni�es) local and regional food systems informing targe�ng and scaling strategies to be used by governments, ins�tu�ons, producer organiza�ons, NGOs, and agri-sector companies.Nutri�on-sensi�ve socioecological-technological (SET) climate-smart innova�ons adapted and co-designed with Agrifood Systems (AFS) actors (farmers, processors, small-medium enterprises (SMEs)), Na�onal Agricultural Research and Extension Systems (NARES) enable local AFS in four LAC countries to effec�vely align the technical aspects of transi�on processes with the socio-ecological needs of at least 50,000 beneficiaries (2022)(2023)(2024).Nutri�on-sensi�ve socio-ecologicaltechnological (SET) climate-smart innova�ons adapted and co-designed with Agrifood Systems (AFS) actors, (farmers, processors, small-medium enterprises (SMEs)), Na�onal Agricultural Research and Extension Systems (NARES) enable local AFS in four LAC countries to effec�vely align the technical aspects of transi�on processes with the socio-ecological needs of at least 50,000 beneficiaries (2022-2024).In 2023, to facilitate farmers' access to climate-resilient and nutrition-sensitive technologies, locally adapted crop varieties such as maize, rice, and beans were validated with 2,207 households in Colombia, Guatemala, and Honduras. Seventeen research platforms (1 per Innova-Hub in Guatemala and Honduras, 5 in Chiapas, 5 in Oaxaca, and 3 in the North Pacific hub of Mexico) were implemented with local research partners to validate sustainable production technologies (output 1.1.1). Information on available climate-resilient, nutrition-sensitive technologies was summarized in technological menus for Oaxaca and Sonora and training material produced on good postharvest practices to minimize grain loss. Two biodiverse plots (one of them involving an indigenous community), were implemented in northern Colombia to compare agroecological practices to conventional methods. The results will contribute to decision-making by producers and to the technological menus (Output 1.1.1).Moreover, 1,584 people participated in AgriLAC Resiliente capacity building on topics including seed systems, agricultural practices, postharvest food processing (four training sessions), and markets (Output 1.1.2). A diagnostic study was carried out for establishing a rice processing plant, aiming to enhance productivity and quality for better access to markets. Additionally, 11 workshops were conducted to strengthen marketing strategies as part of the scaling pathway. The Work Package also developed a prototype product made with cocoa and a protocol for a biofortified rice food product prototype (Output 1.1.3). Producer associa�ons, AgriTech companies, government agencies, NGOs, and public extension services in two LAC countries are empowered by a digitally-enabled ecosystem to offer agro-advisory services to at least 180,000 farmers and other value chain actors to manage climate risk (CRM) more effec�vely and sustainably intensity (SI) produc�on and value chains.Work Package 2 progress against the theory of change AgriLAC Resiliente's Work Package 2 is driving a major digital transformation in agro-climate information service delivery and scaling in Latin America. Work Package 2 made substantial progress in all outputs, intermediary outcomes, and research questions. Related to output 2.1.1, ETL processes to fundamentally change how data are managed by Work Package 2 partners are in place for the Meteorological Service of Guatemala (INSIVUMEH), Institute for Climate Change (ICC), the Meteorological Service of Honduras (CENAOS-COPECO), and the Asociación de Organizaciones de Los Chuchumatanes (ASOCUCH). Well-established partnerships (output 2.1.2) underpin the progress. Substantial progress was also made with the World Food Program network of \"climate monitors\" and rain gauges. Notably, in the case of CENAOS-COPECO, the ETL effort has been implemented together with deploying 10 weather stations specifically targeting \"blind spots\" for meteorological monitoring. These processes have helped build, from the bottom up, many of the technologies and capacities that will underpin the Data Hub. Alongside the ETL and data management transformation efforts, a technological and partner scoping of the Data Hub has begun, delivering a clear roadmap for implementation, with initial focus in Guatemala. The roadmap includes both the technological (output 2.1.1) and the partnership (output 2.1.2) aspects to implement, deploy, and sustain the Data Hub. Continued training in collaboration with Work Package 4 has been critical for the continued improvement and use of e-Agrology. Data analysis of legacy data with an innovative Explainable Machine Learning analytics workflow has generated the first set of recommendations for small-scale Guatemalan farmers. These will be included as part of the MTAs' regional network and climate information delivery and scaling channels (bulletins, WhatsApp, AClimate) and as part of the technological menu of the Innova-Hub (in collaboration with Work Packages 1 and 4). These tools and approaches are all new additions to an already rich digital ecosystem fostered and enhanced by Work Package 2 (output 2.1.3). Lastly, information services (output 2.1.4) are being delivered at scale in Honduras, Guatemala, and Mexico. In Mexico, a recent outcome harvesting study documents the various transformations produced by AgriLAC Resiliente. For Guatemala and Honduras, scientific progress has allowed a comprehensive mapping of information needs, as well as implementation of user research in both Honduras and Guatemala. These efforts are already improving the usability of agro-climate bulletins and helping to drive the design of improved information products for small-scale producers across both countries (see for example: Agroclimatic bulletins for farmers, Radio spots, and Agroclimatic bulletins). All Work Package 2 theory of change assumptions hold thus far. Na�onal and local governments in two LAC countries integrate low-emission strategies with development objec�ves at the agro-ecosystem or value chain level, with an expected impact of ~150,000 ha (2022-2024).Na�onal and local governments in two LAC countries integrate low-omission strategies with development objec�ves at the agro-ecosystem or value chain level with an expected impact of ~150,000 ha (2022)(2023)(2024).Public-private sector, Na�onal Agricultural Research and Extension Systems (NARES), and civil society actors across subna�onal agricultural innova�on systems in three LAC countries use InnovaHub learning, knowledge management, and evidence to understand how to accelerate on-farm uptake of socio-ecologicaltechnological (SET) innova�ons by making them more gender-responsive, produc�on-friendly, and context-specific reaching, at least 15,000 people (2022-2024).During 2023, we combined our efforts with agrifood system stakeholders to develop approaches that integrate climate-change mitigation efforts with those aimed at achieving relevant SDGs in Colombia and Peru. The Work Package also generated data, knowledge, and innovations to address the challenges posed by climate change in territories affected by armed conflict. This was achieved by identifying opportunities that could be enhanced through public policy formulation and implementing actions specifically aimed at contributing to territorial peacebuilding, climate-change mitigation, and improved living conditions.In Peru, access to forests, when coupled with demographic changes and the expansion of agricultural infrastructure, significantly contributes to forest loss. Enhanced accessibility to forests can expedite deforestation by amplifying human activities that directly or indirectly result in depletion of forest resources. In Colombia, we worked in the Caquetá department, where GHG emissions from cocoa and livestock value chains were characterized. In both countries, we identified the potential to reduce emissions from the value-chain production stage. This could become a reality with the help of sustainability strategies for Colombian cocoa chains, which were scaled up to governmental levels and have been validated in Caquetá and Cesar, while Guinean pig value chain was chosen for validation in Junín.We also innovated the way knowledge is transmitted among stakeholders through a Sustainable Cocoa Innovation Challenge in Colombia, Perumin Inspira Challenge in Peru, and the establishment of a knowledge platform named \"EncontrAR,\" which promotes learning and growth among sector entrepreneurs and fosters climate action. This is achieved through collaboration and the contribution of individuals with diverse skills and profiles, providing them with opportunities to improve their technical and business management skills, evaluating potential markets, analyzing financial instruments, and designing a roadmap for accessing sources of funding.Lastly, we have analyzed the relationship between child nutrition problems in Colombia and livestock, finding a significant connection for integrating climate-change mitigation objectives and SDGs. This generates big opportunities for designing effective strategies that simultaneously address climate change mitigation and food and nutrition security objectives, especially in areas in Colombia and Peru with high deforestation rates. Public-private sector, Na�onal Agricultural Research and Extension Systems (NARES), and civil society actors across subna�onal agricultural innova�on systems in three LAC countries use InnovaHub learning, knowledge management, and evidence to understand how to accelerate on-farm uptake of socio-ecological-technological (SET) innova�ons by making them more gender-responsive, produc�on-friendly, and context-specific reaching, at least 15,000 people (2022-2024).Producer associa�ons, AgriTech companies, government agencies, NGOs, and public extension services in two LAC countries are empowered by a digitally-enabled ecosystem to offer agro-advisory services to at least 180,000 farmers and other value chain actors to manage climate risk (CRM) more effec�vely and sustainably intensity (SI) produc�on and value chains.Public and private ins�tu�ons in three LAC countries use CGIAR science, evidence, and tools to inform and shape to Agrifood Systems (AFS) related policies, incen�ves, and ini�a�ves that are more transforma�ve, sustainable, mi�ga�oncomprehensive, and climate adapta�on-friendly (2024-2030).Work Package 4's annual report on InnovaHubs documents collaborations and innovations in Guatemala, Honduras, and Mexico, with intensified characterization studies in Central America. In Guatemala, emphasis is on nutrition and market access, while in Mexico, an infographic guide details InnovaHubs' regional impact. Communication and outreach are critical for scaling innovations and fostering stakeholder engagement through in-field achievements (output 4.1.1).A targeted training strategy bolsters local stakeholder capacities within InnovaHubs, fostering communities of practice among professionals, extensionists, farmers, and scientists to exchange knowledge and best practices (output 4.1.2). The dissemination of agroclimatic information via the MTAs' network in Guatemala and Mexico aims to improve accessibility and comprehension among farmers, aiming to enhance the reach and usefulness of agroclimatic bulletins.Local partners in InnovaHubs provide technical support for farmers through co-learning spaces and extension areas, facilitating the adoption of field recommendations for improved agriculture practices. Furthermore, in Oaxaca, Mexico, the agriculture ministry aligns interventions with InnovaHubs, resulting in over 20,000 registered farmers' fields with researcher support (Output 4.1.3 and Output 4.1.4). A Digital Agricultural plot was established in Guatemala in partnership with the Digital Innovation Initiative, serving as a testing ground for advanced agricultural technologies, benefiting small-scale farmers in the dry corridor (Output 4.1.4).Annual InnovaHub meetings in Guatemala and Honduras facilitated knowledge exchange among agrifood actors, fostering innovation through collaborative learning and adaptation to local contexts. In November 2023, the second round of these meetings occurred in diverse locations across Guatemala and Honduras, gathering 25 and 14 institutions respectively. These gatherings identified local needs and research priorities, strengthening positive impacts, and advancing innovation network management. Agrifood actors collaborate to support capacity development in each InnovaHub's operational space, promoting regional agricultural advancement (Output 4.1.5).Engagement strategy with strategic Na�onal and Regional partners and stakeholders to support AgriLAC's outcomes achievement including scaling and impact-oriented efforts.Comprehensive assessment of migra�on drivers, including socioeconomic, environmental, security, and cultural factors that force or push people to relocate. Integrated Agrifood System Ini�a�ve (IASI) strategies plan to recommend strategies, ac�ons, and quan�ta�ve, SDG-aligned targets with high likelihood of suppor�ve public and private investment.Assessments of Women and youth agricultural prac�ces and adop�on of new technologies given their specific constraints and informa�onal needs while considering their skills, knowledge and aspira�ons.Monitoring and targe�ng tool mapping key interven�ons and actors combined with a comprehensive profile at the subna�onal level to iden�ty opportuni�es to improve investments in agrifood systems.Public and private ins�tu�ons in three LAC countries, use CGIAR science, evidence, and tools to inform and shape to Agrifood Systems (AFS)-related policies, incen�ves, and ini�a�ves that are more transforma�ve, sustainable, mi�ga�on-comprehensive, and climate adapta�on-friendly (2024)(2025)(2026)(2027)(2028)(2029)(2030).Public and private ins�tu�ons in three LAC countries use CGIAR science, evidence, and tools to inform and shape to Agrifood Systems (AFS) related policies, incen�ves, and ini�a�ves that are more transforma�ve, sustainable, mi�ga�on-comprehensive, and climate adapta�on-friendly (2024-2030).In 2023, the Work Package 5 conducted and continued several studies in Guatemala focused on producing insights into climate resilience, migration, and the dynamics of gender roles, and creating tools.Work Package 5 finalized the public policy mapping in Guatemala on food security, climate change, and migration. This identified opportunities and the most feasible applications of the IASI methodology that should lead to customized public policy recommendations. This document underwent a validation process with stakeholders and informed the discussions at the Second National Forum on Migration and Climate Change in Guatemala (output 5.1.4).In addition, Work Package 5 achieved its goal of engaging with AgriLAC Resiliente partners by facilitating consultations at InnovaHubs' annual meetings in Guatemala and Honduras organized by Work Package 4. The consultations aimed to align the Initiative's objectives with partners' needs using a bottom-up approach. (output 5.1.4).The subnational mapping of key programs and interventions in food security and nutrition to identify areas with possible investment gaps and saturation of interventions caught the attention of a group of 13 donors in Guatemala and a related exercise is being performed with them, including developing an interactive platform. This will help to better coordinate and complement efforts between stakeholders and streamline effective use of resources for implementating food security and nutrition actions and programs across the country (output 5.1.3).Two quantitative studies were finalized in Guatemala: i) Cultural and economic barriers and opportunities for the participation of women in agricultural and livestock activities (a collaboration with MELIA published in a special issue and presented at various forums, The team optimized available (reduced) resources to collaboratively deliver a streamlined research approach that developed nutrition-sensitive and climate-smart technologies. This was done with local agrifood system actors in four countries. Working together in a research network is enhancing local capacities from production to market access. It is forming the base of innovation and adaptation in the InnovaHubs (Work Package 4).Progress was made according to plans, with all theory of change assumptions still holding. All four major Work Package 2 outputs are on track, namely, Data Hubs and data architecture modernization, new partnerships and partnership models, a climate risk management toolkit, and improved information services for farmers and small-and mediumsized agricultural enterprises (Ag. SMEs). In particular, we highlight the data management and analysis transformation in INSIVUMEH, ICC, and CENAOS-COPECO, as well as several farmer organizations, and the continued delivery of agroadvisories that stem from MTAs and are scaled through digital channels.The annual progress in Peru and Colombia is largely aligned with the Plan of Results and Budget and the theory of change of Work Package 3. This is evidenced by the development of low-emission sustainable development strategies that integrate the SDGs and help foster the participation of local stakeholders. Progress has also been made in developing instruments to promote markets for investments in climate change mitigation at the value chain level. Planned outputs have been developed, such as those associated with the methodological framework, digital innovations, and science-and market-based solutions.InnovaHubs in Guatemala, Honduras, and Mexico have been consolidated and established. They form the operational base for the subnational actors, including farmers and their associations, local governments, and civil society to operate around a common goal, which includes establishing field infrastructures. Here, technicians and farmers work together to implement and validate best-bet recommendations related to agronomy and climate advisory and digital extension services, as well as local action towards social inclusion. Capacity development schemes are being implemented around this infrastructure and integrate the MTAs' experiences but at a local agroecology scale. Within these, local actors connect and exchange experiences to establish collaborative plans and priorities.Work Package 5 successfully met its objectives for 2023, completing all the planned studies on climate change, migration, and gender, as well as conducting a comprehensive national mapping of public policies in Guatemala. Additionally, Work Package 5 effectively engaged with InnovaHub partners, providing valuable information to support decision-making processes following a bottom-up approach. The subnational mapping exercise of interventions has garnered interest from key stakeholders in the country, significantly contributing to the achievement of the Initiative's outcomes. Overall, the Work Package is on track and all major theory of change assumptions remain valid. 37), as outlined in Figure 1 above. Notably, within the outputs category, there was a significant uptick in the production of knowledge products (125 more than in 2022) and in capacity sharing for development (145 more than in 2022). Moreover, 5 additional innovations were developed in 2023. In the outcomes category, 21 more CGIAR innovations were used in 2023 than in 2022 and 5 new policy changes were documented.Not targeted: The result did not target any of the Impact Area objec�ves.The result has made a significant contribu�on to any of the Impact Area objec�ves, even though the objec�ve(s) is not the principal focus of the result.The result is principally about mee�ng any of the Impact Area objec�ves, and this is fundamental in its design and expected results. The result would not have been undertaken without this objec�ve. The chart above reflects how results of AgriLAC Resiliente reported in 2022 and 2023 have significantly contributed to the five CGIAR impact areas, primarily in Climate change adaptation and mitigation (155), Nutrition, health and food security (36), Gender equality, youth, and social inclusion (25), Environmental health and biodiversity ( 16), and Poverty reduction, livelihoods, and jobs (5).Keywords within the Initiative's knowledge products c l i m a t e c h a n g e AgriLAC's results notably contribute to SDGs, particularly in addressing No Poverty (SDG1), Climate Action (SDG13), and Gender Equality (SDG5), with over 300 contributions each, while also aligning with broader aims to combat hunger, promote sustainable land use, and foster inclusive economic growth. Over the past two years, AgriLAC Resiliente has produced a diverse range of knowledge products, including 70 reports, 11 briefs, 9 journal articles, 6 manuals, 5 working papers, 5 case studies, 3 posters, 2 books, and 1 dataset. These resources serve as valuable repositories of insights into agricultural resilience. Moreover, it has also developed 37 dissemination products, such as presentations, blog posts, videos, audio recordings, brochures, press items, infographics, and newsletters.The innova�on is validated for its ability to achieve a specific impact under uncontrolled condi�onsThe innova�on is tested for its ability to achieve a specific impact under uncontrolled condi�onsThe innova�on is validated for its ability to achieve a specific impact under semi-controlled condi�onsThe innova�on is being tested for its ability to achieve a specific impact under semi-controlled condi�onsThe innova�on is validated for its ability to achieve a specific impact under fully-controlled condi�onsThe innova�on is being tested for its ability to achieve a specific impact under fully-controlled condi�onsThe innova�on's key concepts have been validated for their ability to achieve a specific impact F���������� The innova�on's key concepts are being formulated or designedThe innova�on's basic principles are being researched for their ability to achieve a specific impactThe innova�on is at idea stage AgriLAC has developed 14 innovations, comprising 7 technological, 6 capacity development, and 1 policy, organizational, or institutional innovation. These innovations span incremental (10), disruptive (3), and radical (1) categories. Incremental innovations denote existing innovations undergoing constant progress and improvement, built upon previous CGIAR work in the region. Disruptive innovations introduce new concepts necessitating significant reconfiguration of farming, market, and policy/business models. Radical innovations introduce entirely new products, systems, or services without necessitating major reconfiguration of existing models. Data here represents an overview of reported results in 2022 and 2023. One result can impact multiple countries and can therefore be represented multiple times.AgriLAC Resiliente strengthened the agricultural, climate resilience and market capacities of 4,833 individuals across Latin America and the Caribbean, collaborating with 64 partners (Figure 6 The AgriLAC Resiliente external partner network responds to the systemic and on-demand approach of the Initiative. This network of partners has strong experience and capacities at different levels throughout the Latin America and the Caribbean agrifood system. AgriLAC Resiliente's external partners have been selected based on their previous and current successful collaborations and partnerships with CGIAR as well as their expertise and skills, farmer outreach and delivery capabilities, and capacity to influence public policy.The extensive external partner network boasts an impressive array of over 150 organizations strategically distributed throughout the region. AgriLAC Resiliente has collaborated with government institutions to strengthen their institutional capacities and respond to the demands of public policy with science-driven initiatives (EOIO 2, EOIO 3, EOIO 5). Key collaborations include the ministries of agriculture in Colombia, Guatemala, Honduras, and Mexico, as well as the meteorological services in Guatemala and Honduras. These partnerships are essential to ensure that agricultural policies and strategies are backed by robust scientific evidence and implemented effectively to promote sustainable development in the region. Less resultsConnections are sized by the number of reported results. Collaborations where only one result was reported with a linkage between two Initiatives are excluded.Source: Data extracted from the CGIAR Results Dashboard on 7 March 2024.Portfolio linkages and AgriLAC Resiliente's impact pathways With Climate Resilience, we generated knowledge products related to agroclimatic information through the work of the MTAs, together with the Livestock and Climate Initiative. The information generated by the MTAs increasingly allows more people (especially farmers) access to agroclimatic data, enabling them to make informed and timely field decisions. This information has been disseminated through different mechanisms, such as radio adverts, characterized by their reach to remote rural areas with easily understandable messages, thanks to the comprehensive approach inherent in the human-centered design methodology, WhatsApp, and technicians using this information when supporting farmers.From our collaboration with the Low-Emission Food Systems and National Policies and Strategies Initiatives, we generated knowledge product tools addressing deforestation. This has been through assessing and designing instruments that enabled the construction of two strategies for the sustainability of the cocoa value chain in Cesar and Caquetá in Colombia. These were complemented by a financial analysis of the potential of carbon markets for cocoa production systems, leading to the design of a mixed financial mechanism and evaluation of its potential.These partnerships have been key to promoting the use of innovations, which include biofortified seeds and climate information services among other innovations. Some of the innovations we generated, together with the Digital Innovation Initiative, include the in situ monitoring system in the dry corridor of Guatemala, which provides precise information about climate and soil to enable producers to overcome their unique challenges.Several results were achieved through the contribution from non-pooled projects. With the Fortalecimiento de Resiliencia de la Producción de Maíz en Guatemala project (International Cooperation and Development Fund), we were able to develop an R package to provide users with convenient access to a wide range of agroclimatic forecasts offered in the AClimate platform. With the ProResiliencia project (European Union funder), we continued the use of Participatory Integrated Climate Services for Agriculture (PICSA). This is a knowledge product that proposes an approach of agricultural extension and climate services. This product helps farmers to formulate plans and decisions adapted to individual farmer contexts according to their production system. This highlights the importance of collaborating with other components of CGIAR portfolio, to increase our reach and impact.Section 7: Adaptive managementModify EOIO 2 by increasing the number of countries from two to three.EOIO 2: Producer associations, AgriTech companies, government agencies, NGOs, and public extension services in three LAC countries are empowered by a digitally enabled ecosystem to offer agro-advisory services to at least 180.000 farmers and other value chain actors to manage climate risk more effectively and sustainably intensify production and value chains.The adjustments stem from the scaling efforts undertaken by the Agriculture Secretariat (SADER) in Mexico. These efforts have enabled Work Package 2 to leverage the MTAs and the InnovaHub network of agroclimatic information services, thereby gaining deeper insights into the network and collaborating with partners to improve the delivery of agroclimatic services. Furthermore, the adjustments align with a more precise identification of the capacities of organizations capable of participating in digital agriculture initiatives.Modify EOIO 3 by adjusting the narrative to incorporate two strategies instead of two countries.Two national or local governments or key stakeholders in LAC countries integrate low-emission strategies with development objectives at the agro-ecosystem or value chain level, with an expected impact of around 150,000 ha.We are giving more emphasis to the number of strategies than the number of countries. These changes are proposed in the context of budgetary changes, and our capacity to align bilateral funding toward accomplishing the outcome.Modify EOIO 5 by decreasing the number of countries from three to two.Public and private institutions in two LAC countries use CGIAR science, evidence, and tools to inform and shape agrifood system-related policies, incentives, and initiatives that are more transformative, sustainable, mitigation-comprehensive, and climate adaptation-friendly (2024-2030).The adjustment in the EOIO stems from budget reallocations, which necessitated prioritizing specific activities within certain countries, and the time needed to produce essential outputs such as knowledge products, instruments, and tools. These outputs play a crucial role in informing local stakeholders and have the potential to shape initiatives and policies in the designated countries.Cacao training on harvest and post-harvest processes, San Vicente Chucurí, Colombia. Credit: Juan Pablo Marín.Section 8: Key result story For many years, the states of Cesar and Caquetá in Colombia have suffered severely from armed violence. This has led to forced displacement and a slowdown in various economic activities, with coordination between public and private entities and farmers' associations to implement effective development plans in the cocoa value chain hindered.A sustainable land use system (SLUS) project and CGIAR's AgriLAC Resiliente and Low-Emission Food Systems Initiatives have worked together to i) consolidate and empower communities through cocoa committees; ii) coordinate interinstitutional interactions, and iii) formalize processes to position cocoa cultivation as an alternative way to reforest and restore degraded landscapes. These activities have supported land restitution and formalization, forest conservation, and the development of sustainable business models based on cocoa cultivation to increase carbon storage and improve rural livelihoods.Establishing close coordination between the key local and national actors in Colombia's cocoa sector was an imperative. The Ministry of Agriculture, the Ministry of the Environment, the Departmental Secretariats, the National Cocoa Council, the cocoa industry, research institutions, marketers and producers continue to work together to further scale and improve the value chain with a clear and sustainable strategic plan. In it, work continues to expand training in low-emissions practices, with a focus on sustainable land use systems such as cocoa agroforestry and silvopastoral production-systems that can enhance people's livelihoods while protecting their environments.This process aims to reconcile climate-change mitigation objectives with development objectives, integrating i) the territorial approach, focused on local decisions on land use, and ii) the market approach, which considers the dynamics of the value chain and consumer decisions in sustainable food systems. This approach will continue to add the participation of more actors with compatible business models. In turn this will overcome identified farm-and value-chainlevel barriers, in addition to measuring the contribution to climatechange mitigation and peacebuilding in the country.Promoting sustainable cocoa involves increasing its production and ensuring that it is done efficiently and sustainably. This includes expanding distribution channels for certified plant material, which guarantees not only crop quality but also its long-term sustainability. Furthermore, the exchange of technical knowledge and institutional strengthening is crucial for identifying and prioritizing needs and establishing specific actions that contribute to the sustainable development of the region, thus fostering local economic growth and improving the livelihoods of cocoa farmers. By strengthening the coordination among actors in the production chain, a solid foundation is created that facilitates continuous strengthening and effective management. This strategic collaboration not only benefits farmers by improving their practices and increasing their productivity but also promotes sustainable practices and more responsible management of natural resources.Thus, the sustainable development of crops and rural communities depends to a large extent on the promotion of economic activities such as cocoa cultivation carried out in productive and environmentally friendly ways. This requires not only efficient crop management but also institutional measures that strengthen infrastructure and generate equitable opportunities for all involved. By prioritizing sustainability and equity in rural development, we can build a more prosperous and harmonious future for communities, where agriculture and environmental conservation go hand in hand.Cocoa has become an opportunity to generate income, to put down roots, and to start over-with more knowledge, more care for the environment, more sense of belonging and more trust in farmers, including those who were displaced by violence. ","tokenCount":"7163"} \ No newline at end of file diff --git a/data/part_6/0882612921.json b/data/part_6/0882612921.json new file mode 100644 index 0000000000000000000000000000000000000000..1ea32a8885a19e832e93bb180f2f93cd48570494 --- /dev/null +++ b/data/part_6/0882612921.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"1e0eaba600627fae94135c73ef9e2cb5","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/fc621161-b58f-41cc-8375-02cdc68db2fd/retrieve","id":"-1279977896"},"keywords":[],"sieverID":"0539e3d7-8764-4709-9013-46be1e99ae82","pagecount":"32","content":"6. The Casamance is characterized by forests and savannah with trees (CIRAD, 2015). Its agricultural production includes mainly rain-fed rice along with diverse other crops. With a total surface area of 28,324 km2, it is divided into three zones-lower, middle, and upper. The region faces challenges such as lowland soil acidification, water erosion, a loss of forest diversity, increased soil salinization, iron toxicity, and acute mangrove degradation (Alessandro et al., 2015).FIGURE 1: Agro-ecological zones of Senegal (left), cropped areas (center), and areas with pastures (right). Data for the left panel were adapted from the Directorate of Water, Forests and Hunting Conservation, and the center and right panels were taken from Ramankutty et al., 2008. - -------------------------------------------------------------------------------------------------FIGURE 2: Current crop suitability in Senegal - --------------------------------------------------------------------FIGURE 3: Changes in suitability for selected value chains by the 2050s for RCP 4.5. 1.0 denotes increasing suitability and -1.0 a loss in suitability. ---------------------------------------------FIGURE 4: Spatial distribution of natural hazards across Senegal - --------------------------------------- -----------------------FIGURE 8: Vulnerability difference between RCP 4.5 and RCP 8.5 for 2050s --------------------------FIGURE 9: Contribution of different agricultural value chains to overall climate change vulnerability by the 2050s for RCP 4.5. Supplementary Figure S4 in the Annex show results for RCP 8.5 by the 2050s.-------------------------------------------------------------------------------------------------FIGURE S1. Crop suitability of the nine value chains by the 2050s for RCP 4.5 ----------------------- -------------------------------------------------------------------------------------------------------TABLES TABLE 1: Key natural hazards in Senegal - --------------------------------------------------------------------------TABLE 2: Adaptive capacity variables used in the vulnerability assessment for Senegal --------viiThe Adaptation and Valorization of Entrepreneurship in Irrigated Agriculture (AVENIR) project aims to improve the socioeconomic well-being and resilience of farming households in the regions of Sedhiou and Tambacounda, Senegal. The project focuses on smallholder-irrigated systems through promotion of climate-adapted irrigation and agricultural practices, particularly for women and young people. AVENIR seeks to promote crop diversification through the integration of rice, agroforestry, and horticulture. In Goudiry and Tambacounda Departments within Tambacounda Region, the project focuses on rice, baobab and horticulture value chains. In Bounkiling and Goudomp departments within Sédhiou Region, the project focuses on rice, mango, cashew, and horticulture value chains. Among the important associated crops prioritized in the two regions are ditakh (Detarium senegalense), madd (Saba senegalensis), onion, okra, and pepper.The vulnerability assessment for the selected crops in Senegal is based on the interaction of sensitivity to change, exposure, and adaptive capacity. We use the conceptual framework of climate-related risk from the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC) Working Group II (WGII) to examine the impacts that climate change is likely to have on agriculture and food security. The ultimate purpose of this study is to assess if the future climate has a neutral (no change), negative (decreasing), or positive (increasing) impact on crop productivity, and to identify regions of concern and opportunities for climate change adaptation. We used the Maxent ecological models under intermediate and high-emission climate scenarios -Representative Concentration Pathways (RCPs) 4.5 and 8.5, respectively -to assess the sensitivity of nine crops to climate change: rice, baobab, cashew, mango, okra, onion, pepper, madd and ditakh. To produce a crop-specific vulnerability index and a final accumulative score, we combined the components of vulnerability using equal weighting. We have also mapped the hotspots of climate change vulnerability and identified the underlying driving indicators. For example, we found that the south, east, and southeastern regions are most vulnerable, especially Tambacounda, Kaffrine, Sedhiou, Kolda, and Kedougou regions. There is a high vulnerability for baobab trees and cashew to the north, as well as cashews, ditakh, okra, onions, and rice to the northeast. This study highlights how the adaptive capacity of the farming population can be enhanced by augmenting access to education and health services, improving nutrition, and developing infrastructure for marketing, transportation, and irrigation.Senegal is in western Africa on the Atlantic Coast between the latitudes of 12°30° and 16°30°N and the longitudes of 11°30° and 17°30°W. The southern region of Senegal has a sub-tropical climate, while the northern region lies in a sub-tropical, semi-arid belt called the Sahel (Mcsweeney et al., 2008). Rainfall is mainly controlled by the movement of the Intertropical Convergence Zone (ITCZ) (Lucio, 2012). The movement of the ITCZ determines the onset and duration of the rainy season (Salack et al., 2011). For example, the south has more rainy days and a longer rainy season than the drier north.Temperatures in Senegal exhibit an east-to-west gradient, such that inland temperatures are normally higher than along the coastline, with the highest temperatures occurring in the northeastern parts of the country (Fall et al., 2006). Matam region located in the northeast, for instance, experiences a maximum temperature of above 40°C in the hottest month of May. The coastal regions, on the other hand, experience cooler temperatures between 25°C to 28°C (Mcsweeney et al., 2008). In the cooler seasons, average temperatures can fall below 25°C at the coast but still climb to 30°C in the east (Fall et al., 2006).Rainfall begins in the southeast around May or June, and spreads northwest throughout the summer months through September (Marteau et al., 2009). August accumulates the highest amount of rainfall (Camberlin and Diop, 2003). The dry season, on the other hand, lasts for about six months in the south and eight months in the north. The highest seasonal rainfall is received in the southern parts of the country, measuring approximately 1000 mm, while the northern parts receive less than 400 mm (USAID, 2017(USAID, , 2015)). The rainy season ceases with the migration of the ITCZ to the south around October (Nicholson, 2018).The agricultural sector of Senegal contributes about 17% of the country's gross domestic product (GDP), employing more than 70% of the workforce (World Bank, 2018). Senegal is one of the most stable and promising countries in West Africa with great potential to increase its agriculture-led economic growth (USAID, 2015). However, a large portion of Senegal's landmass lies within the Sahel, which is arid and highly prone to droughts (Mcsweeney et al., 2008). This location makes rain-fed agricultural production highly variable, a situation that climate change is exacerbating (D ' Alessandro et al., 2015).Senegal is divided into six agro-ecological zones (Figure 1) based on biophysical and socioeconomic characteristics (Alessandro et al., 2015;CIRAD, 2015). Moving from north to south, these are the following:1. The Senegal River Valley characterized by alluvial plains and sandy uplands with irrigated rice production; it covers a surface area of 9,658 km 2 . Most agricultural production occurs with irrigation (Alessandro et al., 2015). Although salinity is a problem in some areas, much of the land has high fertility levels because of regular flooding and siltation.2. The Niayes on the Atlantic coast features a temperate climate and produces fruits and vegetables (CIRAD, 2015). This 100km -280km strip occupies 2,759 km 2 . Niayes is a densely populated area and faces challenges including soil and water salinity and coastal erosion (Alessandro et al., 2015).3. The sylvo-pastoral zone of north-central Senegal supports extensive livestock production and covers 55,561 km 2 (CIRAD, 2015).4. The Groundnut Basin of south-central Senegal is a zone of savannah dominated by groundnut and millet production (CIRAD, 2015). It covers an area of 46,367 km 2 and is densely populated. Ecosystem degradation and depletion of land resources, mainly soil fertility and timber resources, affect the area (Alessandro et al., 2015). Because of upland soil acidification and lowland salinity, soil regeneration has declined.5. Eastern Senegal is characterized by savannah with trees. Its agricultural production involves primarily cotton and livestock. It covers an area of 56 529km 2 (Tappan et al., 2004) and is subject to rampant rural poverty because of extreme population pressure on natural resources, despite its robust agropastoral potential (Alessandro et al., 2015).La Casamance est caractérisée par des forêts et des savanes arborées (CIRAD, 2015). Sa production agricole comprend principalement le riz pluvial ainsi que diverses autres cultures. Avec une superficie totale de 28 324 km2, elle est divisée en trois zones -basse, moyenne et haute. La région est confrontée à des défis tels que l'acidification des sols au niveau des zones basses, l'érosion de l'eau, la perte de la diversité forestière, la salinisation accrue des sols, la toxicité ferreuse et la dégradation aiguë des mangroves (Alessandro et al., 2015). The topography of Senegal is generally flat with rolling sandy plains but rises to hills in the southeast.Most areas have elevations of less than 100 m above sea level. Senegal encompasses over 19 million ha of land, of which only about 20% or 3.9 million ha are suitable for arable crops. The rest comprises undeveloped bush and arid areas used for livestock grazing (Alessandro et al., 2015). Around 40% of the arable land is constantly cultivated, although 10% receives less than 500 mm of rainfall per year, which limits crop production. Only 10% of the cultivated land is under irrigation, mainly along the Senegal River and in the Casamance (Peterson et al., 2006). This challenge aligns with the goal of the AVENIR project, which aims to improve access for irrigation technologies and improve the governance and management of water resources, working with government, civic groups, and market actors in Sédhiou and Tambacounda regions.Population increases have led to land pressure (Place and Otsuka, 2000), which in turn has brought about soil degradation and declining soil fertility due to many years of unsuitable agricultural practices such as tillage practices, mono-cropping, and incorrect use of chemical inputs (Doso Jnr, 2014;Sow et al., 2015). Soils in most areas have low percentages of clay and organic matter and therefore low cation exchange capacities, resulting in increased vulnerability to nutrient depletion (Mahé et al., 2002;Matlon, 1987).Millet, rice, maize, and sorghum are the major food crops grown in Senegal. Other crops such as groundnuts, sugarcane and cotton are important cash crops. A wide variety of fruits and vegetables are grown for local and export markets. Cowpeas and cotton are also cultivated. Because food production does not meet domestic demand, the country imports rice and wheat (Diagne et al., 2013). Senegal exports cotton, groundnuts, and horticultural products, mainly green beans, tomatoes, cashew and mangoes (D 'Alessandro et al., 2015).Crop production in Senegal falls into several categories: subsistence smallholders, commercial smallholders, and pure commercial producers (D' Alessandro et al., 2015). Subsistence smallholders produce food mainly for consumption with occasional surplus for sale, while commercial smallholders produce cash crops for sale and some food crops for their own consumption. About 90% of the rural population of Senegal are involved in livestock production, which accounts for 30% of the country's GDP (Diagne et al., 2013). Livestock includes cattle, goats, sheep, and poultry farming. The cattle provide plowing power which is used in cropped lands (D' Alessandro et al., 2015).Senegal is highly vulnerable to risks associated with climate change (USAID, 2017). Years of erratic rainfall patterns and rising sea level has led to increased soil erosion, agricultural soil salinization, and the destruction of infrastructure. Droughts and floods associated with climate change have increased the country's vulnerability to food security (Fall, 2020). In the Senegal River Valley, the Niayes, and the Casamance, agriculture and fisheries are some of the main economic activities, and they are highly vulnerable to reductions in rainfall, coastal erosion, salt water intrusion, and flooding (Fall, 2020).The methodology used to prepare this report hinges on the conceptual framework of climate-related risk from the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC) Working Group II to explore the potential consequences of climate change for agriculture and food security (Adger, 2006;O'brien et al., 2007;Sharma and Ravindranath, 2019). The IPCC defines vulnerability as \"the extent to which a natural or social system is susceptible to sustaining damage from climate change impacts, and is a function of exposure, sensitivity and adaptive capacity\". The impact of climate change on agriculture and livelihoods therefore can be conceptualized as the aggregation of these components (Foden et al., 2013).Exposure refers to the amount of climate variation to which a system could be subjected by hazards. Sensitivity, meanwhile, is the degree to which the system could be affected by that exposure. Finally, adaptive capacity is the ability to adjust, cope with, or benefit from expected climate variations. The analysis was implemented by obtaining indicators relevant to Senegal for each dimension of vulnerability. These indicators were then aggregated as shown in equation 1 below to compute the vulnerability of each crop and administrative unit or arrondissement.In the vulnerability framework we use here, the vulnerability of each crop is calculated using crop-specific sensitivities, exposure to natural hazards, and a series of indicators of adaptive capacity (equation 1), and the results are then summed up to obtain overall vulnerability (Parker et al. 2019). Where i denotes each crop; Growing area refers to the extent of suitable area for the crop i; Total area pertains to all crops; S i is the sensitivity of the value chain i; Ei represents the exposure of each crop; and AC is the adaptive capacity.Sensitivity index, Si, was determined by computing the difference between the future and current crop suitability followed by normalizing the values to a scale ranging from -1 and 1. Exposure indices, Ei was determined by obtaining the variables representing the value chains exposure such as aridity, flood etc. and extracting the values for each arrondissement. The resulting values were then normalized to a scale ranging from 0 and 1. Adaptive capacity, AC on the other hand was determined by obtaining the variables that enhance the adaptive capacity of Senegal such as literacy rate, health, poverty etc. and extracting the values for each arrondissement. The resulting values were also normalized to a scale ranging from 0 and 1. Crop suitability was modelled for nine selected crops: baobab, cashew, ditakh, madd, mango, onion, okra, pepper, and rice, using the Maxent suitability model package in R Statistical software (Figure 2). These crops were selected from a value chain study conducted for the AVENIR project in February to March 2020, which identified these as the most promising crops for socio-economic progress in the regions of Tambacounda and Sedhiou. To obtain one index for each variable in the equation e.g., one index for AC in calculating total vulnerability for a specific crop, all indices were added and normalized to a scale between 0 and 1.Crop suitability determines the effectiveness of a specific area for the production of a particular crop within a defined system of agricultural production based on agro-climatic conditions related to temperature and moisture, and on agro-edaphic conditions pertaining to soils and landforms (Kassam et al., 2012;Nisar Ahamed et al., 2000).We carry out a Maxent suitability model using available crop presence data and gridded climate data for current and future scenarios pertaining to the 2050s, and for two Representative Concentration Pathways (RCPs): RCP 4.5 and RCP 8.5. The RCPs describe various climate futures whose likelihood of occurring depends upon the volume of anthropogenic greenhouse gases (GHG) emitted over the years. RCP 4.5 is an intermediate scenario premised on the employment of a range of technologies and strategies for reducing GHG emissions. RCP 8.5 is a high-end scenario characterized by increasing GHG emissions over time. Our value chain presence data were derived from two sources; the Global Biodiversity Information Facility public database (http://www.gbif.org) as well as on farm observations of value chain presence obtained from local extension officers. The variables we use include climate data with bio-climatic variables relating to temperature in addition to slope, soil texture, soil pH, and waterlogging. We obtained current climate data from WorldClim 2.0 (Fick and Hijmans, 2017), whereas we downloaded future climate data from CCAFS-Climate (Navarro-Racines et al., 2020). We calculated slope data from a digital elevation model obtained from the United States Geological Survey's Earth Explorer and determined soil texture and pH from the International Soil Reference and Information Centre World Soil Information database. Finally, information on waterlogging is obtained from the United Nations Food and Agriculture Organization (FAO) Global Assessment of Soil Degradation (Fischer et al., 2008).We present this information in two ways: (1) the current (baseline) magnitude of suitability; (2) the magnitude of change under future climate projections. The magnitude of change reflects whether crop suitability will increase or decrease relative to the baseline period. In the following section, we show agreement maps that rank the current suitability from zero, meaning areas are projected to be climatically unsuitable for production of the crop, to one, where the area presents greatest climatic conditions suitable for crop production.The current suitability of the selected crops in the project areas, show that, cashew, ditakh, madd, mango, and rice have overall high suitability under current climate in Sedhiou, while pepper will have moderate to high suitability. In Tambacounda, pepper has moderate suitability. Baobab, onion and rice show high suitability to the northeastern areas bordering Mali, and low suitability in other areas of the region. Madd shows high suitability in the southern part. Rice in Tambacounda is suitable along the Senegal River to the east as well as to the south near Kolda region. Okra shows low suitability in both regions. Baobab and onion show very low suitability in Sédhiou while ditakh and cashew show low suitability in Tambacounda. Sensitivity expresses the relationship between human-induced emissions and the temperature changes that will result from these emissions. It is the amount of warming caused by increases in atmospheric carbon dioxide (CO2) (Hawkins and Forster, 2019). Frequently, sensitivity is defined as the change in temperature resulting from a doubling of the concentration of CO2 in the atmosphere. In this case, we calculated sensitivity using crop suitability such that we understood sensitivity as the change in suitability. Therefore, we computed the difference between the future and current crop suitability.We analyzed the sensitivity of all crops under future climate projections for 2050s under RCP 4.5 (Figure 3). All the selected crops experience some degree of sensitivity, either an increase or decrease. Increasing sensitivity infers that climate change will affect productivity patterns for the crop. The change in suitability maps range from -1 meaning areas with 100 percent crop suitability loss, to +1 representing areas where there are 100% suitability increases. By the 2050s under RCP 4.5, cashew, pepper, and rice are the most sensitive crops, while baobab, mango, okra and onion are least sensitive. In this scenario, increasing suitability is strongly exhibited by baobab and okra in most areas of Senegal, cashew and ditakh towards central Senegal and madd to the southeast and central Senegal. The suitability of onion along the Senegal river valley and in the Casamance especially Kolda region is seen to increase with the suitability decreasing to the west of the country. Decreasing suitability is likely for rice along the Senegal River valley as well as in Sédhiou with increase in suitability in the Casamance areas shifting to Kolda region to the east of Sédhiou. There is a decreasing suitability for pepper in the Casamance, Tambacounda, Kedougou and the western part of the country with an increasing suitability in the central areas.Changes in suitability for selected value chains by the 2050s for RCP 4.5. 1.0 denotes increasing suitability and -1.0 a loss in suitability.For the AVENIR project areas, there is a likelihood for a decreasing suitability in rice, pepper, madd, cashew and ditakh in Sédhiou with an increasing suitability for mango to the north and okra. Pockets of increasing suitability for Cashew and ditakh are seen across the region. Although baobab is not currently suitable in this region, further reduction in suitability is exhibited in this scenario. In Tambacounda, there is a likelihood for an increase in suitability in okra, baobab to the east, cashew and ditakh to the west and east and mango and onion to the east which could lead to an increase in production by 2050 for RCP 4.5. Cashew and ditakh however, show decreasing suitability in central Tambacounda while madd, pepper and rice show a high decrease in suitability by 2050 for RCP4.5.Results for RCP 8.5 for 2050s are shown in the supplementary figures in the Annex. The results show an increasing suitability for baobab and okra with increases in the suitability of cashew, ditakh, and madd towards the east. The suitability of mango, okra and onion shifts towards the north of Senegal, while pepper suitability increases towards central, east and south eastern parts of the country. The suitability of rice on the other hand, decreases along the Senegal river valley but increases towards the central parts of the country and in the Casamance and more so in Sédhiou. This shows a high probability of these value chains shifting form the current production areas which might lead to a decrease in production in the areas currently under production and consequently food insecurity. This calls for production consideration of these new areas.Senegal remains vulnerable to climatic shocks including natural hazards that are predicted to increase in magnitude and extent because of climate variability (Simonet and Jobbins, 2016).These hazards include droughts and floods, which recur seasonally, affecting livelihoods. Increasing precipitation and rising sea level pose a great risk to people living in coastal, urban areas, who account for approximately 67% of Senegal's population (Croitoru et al., 2019;USAID, 2011). Variation in the start of the growing season, meanwhile, increases the vulnerability of farmers who lack access to irrigation because they are unable to schedule the timing of cropping activities such as planting and harvesting. In assessing vulnerability, we mapped natural hazards as shown in Table 1 below.Droughts experienced in the 1970s and 1980s contributed to food insecurity in Senegal and the Sahel in general (USAID, 2017). Recent drought events in 2000 led to a 74% decline in groundnut revenues and diminished revenues for millet and sorghum by 60% (World Bank, 2011). More droughts occurred in 2002, affecting 284,000 people;in 2006/2007;and2011, affecting 806,000 people (WFP, 2013). Droughts in 2014, 2017, and in 2018 impacted 245,000 people (Bhaga et al., 2020).Floods, on the other hand, have become frequent due to increasing heavy rainfall events (USAID, 2017). Between 2000 and 2012, damages resulting from floods occurred in at least 8 years. In 2002, 179,000 people were affected;in 2008, 250,000 people;andin 2009, 360,000 people (WFP, 2013). The 2012 floods along the Senegal River and in low-lying areas of Greater Dakar affected over 265,000 people, exacerbating the flood-induced food security crisis of 2011/2012 (WFP, 2012). More recently, 2020 received higher-than-normal rainfall that led to flooding (ReliefWeb, 2020). In the absence of mitigation practices, consecutive floods and droughts can cause severe land degradation, worsening the crisis of food insecurity. Land degradation in Senegal is linked to the salinization of agricultural land, especially of rice paddies; water erosion that causes stripping and gullying; and to wind erosion that removes the surface layer of soils and destroys its potential for production (Sow et al., 2016).We extracted the data in raster format as an average for each arrondissement of Senegal and then normalized to a scale from 0 to 1. Senegal is subdivided into regions, which are further subdivided into departments and arrondissements. For each arrondissement, this data was later used to calculate vulnerability by applying equation 1. Figure 4 shows the spatial distribution of the six natural hazards considered: aridity, erosion, fire, flood, salinization, and waterlogging. Aridity affects the northern areas of Senegal, fire the southeastern areas, and erosion the central areas. Flooding and salinization are particularly impactful in the southwestern areas of Ziguinchor and Sedhiou and in areas close to the coast. Waterlogging is mostly experienced in the southern areas of Senegal, such as the Casamance, which also receives high levels of rainfall. Exposure to arid conditions is particularly acute in the north and less severe in the south.We calculated changes in temperature for the four scenarios to show the rate at which temperatures in Senegal are projected to increase (Figure 5). By the 2050s for RCP 4.5, temperatures are projected to increase slightly in eastern and southeastern Senegal, with the highest increase in Tambacounda Region. In some parts of Thiès and Louga Regions, temperatures will remain similar to the present conditions. Even greater temperature changes are projected under RCP 8.5 for the 2050s, particularly for the east, south, and southeastern areas of Senegal. Some arrondissements in Tambacounda Region will experience the greatest increases in temperature. Changes in temperature signify heat stress, which negatively impacts agriculture, particularly for livestock. Adaptive capacity refers to the ability of a system to prepare for climate stresses and changes in advance (Smit et al., 2003) or the ability to adjust and respond to the effects caused by climate change (IPCC, 2014). Increased adaptive capacity means better opportunities for systems to manage climate impacts of varying magnitudes (IPCC, 2012). For this vulnerability analysis, we gathered geospatial data on the variables that enhance adaptive capacity in Senegal (Table 2). We then normalized these variables so that 0 indicates lack of adaptive capacity and 1 corresponds to absolute adaptive capacity. For example, poverty was reciprocated so that a higher value shows low adaptive capacity.Variable DescriptionObtained by counting the total number of crops that are growing in each arrondissement. Crop distribution areas were taken from MapSPAM (You et al., 2017).The percentage of the population in each age group that can read and write. The adult literacy rate was obtained from the proportion of adults who had accessed primary education.The ratio of the number of children aged 0-14 years and older persons aged 65 years or above, to the working-age population between 15 and 64 years old (UN, 2006).Access to health care Distance to health care facilities (Maina et al., 2019).The institutional capital index relates to the \"governance index\", \"conflictuality index\", and \"environmental management index\" of an institution (ClimAfrica, 2014).Travel time to major cities is used as a measure of the accessibility to markets.The proportion of the population living in households below the international poverty line, where the average daily consumption or income per person is less than $1.25 a day measured at 2005 international prices adjusted for purchasing power parity.The percentage of children under 5 years old whose standard score (z-score) falls below -2 standard deviations from the median height-for-age according to the World Health Organization (WHO) Child Growth Standards.The percentage of children under 5 years old whose standard score (z-score) falls below -2 standard deviations from the median weight-for-height according to the WHO Child Growth Standards.The percentage of children under 5 years old whose standard score (z-score) falls above +2 standard deviations from the median weight-for-age according to the WHO Child Growth Standards.Technological capacity A combination of two underlying indexes: the \"household technology index\" and the \"infrastructure index\". Technological capacity is linked with the diffusion of basic life technology and infrastructure, is linked to transportation networks.Distance to the nearest water body. This variable represents access to irrigation and water for household consumption.Under-5 mortality Refers to the probability of dying between birth and exactly five years of age, expressed per 1,000 live births.Percentage of the population living with HIV per 1000 people.People experiencing insufficient food consumption. This variable is expressed as poor or borderline food consumption, according to the Food Consumption Score.Areas considered to have high potential for irrigation by their closeness to water sources, and areas with soils containing more clay are also more suitable for irrigation because clay improves soil's water holding capacity.Under-5 mortality from malaria infections.Access to electrical energy (Falchetta et al., 2019).Residual water irrigation potential Areas where water is predicted to remain on the soil longer after rainfall. This variable is estimated using data such as slope and soil's water-holding capacity, clay, and organic carbon content.Soil fertility Soil fertility index (Lu et al., 2002).Phone access Number of households owning a mobile phone.The indicators of adaptive capacity represented here varied greatly across Senegal's various regions and arrondissements. For instance, some arrondissements had very low adaptive capacity associated with poverty, but high adaptive capacity associated with crop diversification and access to hospitals, markets, and irrigable areas. Rates of stunting, underweight children, wasting, malaria, and poverty are generally high in the western parts of the country. In addition, access to health care, water, markets, and soil fertility are low in western areas. These variables improve in the east and toward Dakar. Regions around Dakar and Ziguinchor have lower rates of stunting, underweight children, and wasting, and better access to health care, water, and markets. These regions are also characterized by low poverty rates and higher levels of soil fertility. In comparison to western Senegal, eastern regions face high rates of HIV prevalence and low access to electricity (Figures 6a and 6b). By applying equation 1 using all the indicators for sensitivity, exposure, and adaptive capacity, we computed the vulnerability of the Senegalese agricultural sector to climate change. Our analysis focused on the future climate for RCP 4.5, an intermediate scenario that assumes partial implementation of the Paris Agreement, and RCP 8.5, the business-as-usual scenario, for the 2050s.Overall crop vulnerability in the 2050s under RCP 4.5 is greatest in the regions of Kaffrine, Tambacounda, Sedhiou, Kolda, and Kedougou, which are mainly in the central, southern, and southeastern parts of Senegal. There are also other specific arrondissements with high vulnerability elsewhere across the country (Figure 7a). On the other hand, vulnerability is lowest in areas near Dakar and Thiès. Some areas in the north, especially in the regions of Thiès and Louga, have lower vulnerability to climate change, probably because of the ease of access to markets, high technological and institutional capacity.For RCP 8.5 in the 2050s, vulnerability to climate change increases as compared to RCP 4.5 (Figure 7b) but remains higher in the regions of Kaffrine, Tambacounda, Sedhiou, Kolda, and Kedougou. Under RCP 8.5 for the 2050s, climate change will lead to increased vulnerability especially in the northeastern areas of Senegal on top of the areas that already have high vulnerability (Figure 7b). Vulnerability comparison between RCP 4.5 and RCP 8.5 show an increase in vulnerability for all regions of Senegal for RCP8.5 compared to RCP4.5 (Figure 8). This shows that in the RCP 8.5, which is an unlikely high-risk future, Senegal regions will be more vulnerable to the effects of climate change compared to RCP 4.5, which gives a more optimistic future. The highest increase in vulnerability is exhibited in the arrondissements of Sédhiou except one to the north while the lowest is seen in most arrondissements of Kedougou and Tambacounda. Other regions with lower increase in vulnerability include Zinguinchor, Kaolack, Fatick and Thiés.FIGURE 8: Vulnerability difference between RCP 4.5 and RCP 8.5 for 2050sIn the areas of greatest vulnerability, that is, the central, southern, and southeastern parts of Senegal, most of the crops under analysis faced a significant reduction in suitability. These areas also experience heightened poverty rates, limited access to health facilities, high rates of stunted, wasted, and underweight children, and elevated malaria mortality. In additions, these areas have limited access to markets, depressed literacy rates, significant food insecurity, and low technological capacity. However, these areas also promise the greatest opportunity for farming using irrigation and residual soil moisture.All nine crops under analysis are most vulnerable towards southeastern Senegal, in the regions of Tambacounda, Kaffrine, Sedhiou, Kolda, and Kedougou but with madd exhibiting a lower value of vulnerability compared to other value chains. In the north, baobab trees are slightly vulnerable. Okra is slightly vulnerable to the northwest; onion to the northeast and rice to the northeast along the Senegal river valley. Mangoes, madd and pepper are the least vulnerable crops in Senegal (Figure 9). This situation is attributed to heightened climatic variations in the east and south of Senegal that leads to overall declines in crop suitability. More than 70% of Senegal's population depend on agriculture, especially in the rural areas. With increasing youth populations, it is essential to empower young people with technical skills for profitable entrepreneurship and employment opportunities in agricultural value chains. The lack of economic opportunities is a key driver for outmigration in Senegal. Despite having about 1.5 million hectares of cultivated land and the potential to irrigate up to 240,000 hectares, at present the country irrigates only 10 percent of the cultivated area.The vulnerability assessment for the selected crops in Senegal is based on their sensitivity to climate change, exposure, and adaptive capacities. We identified drought, soil erosion, flooding, fires, salinization and waterlogging as the main natural hazards representing exposure to climate change in Senegal.The adaptive capacity of the farming population can be enhanced by boosting literacy rates; increasing access to educational institutions and health facilities; improving nutrition outcomes to reduce the rates of stunted, wasted, and underweight children; and developing marketing, transportation, and irrigation infrastructure. Priority areas for ameliorating crop vulnerability include the eastern, southern, and southeastern areas of Senegal, which encompass Kaffrine, Tambacounda, Sedhiou, Zinguinchor, Diourbel, Fatick, Kolda, and Kedougou Regions.Under climate change, there will be a decline in crop suitability for all the nine value chains especially in the regions to the south and the southeast of Senegal. There is also a notable shift in suitability for cashews, ditakh and madd towards the east and okra and onions towards the north. Various climate smart interventions will therefore be of most importance to reduce the effects of climate change on the selected value chains. Adoption of practices such as reforestation as well as technologies that increase crop cover and reduces erosion could enhance soil water retention. Other opportunities include irrigation and the harvesting of flood waters for cultivation.Agricultural interventions for improved nutrition including diversifying crops for healthier diets, increasing production for food and nutrition security and communication strategies to promote positive changes in knowledge, attitudes, norms, beliefs and behaviors will enhance the ability to adjust, cope or benefit from the expected climate variations across the country. ","tokenCount":"5569"} \ No newline at end of file diff --git a/data/part_6/0885203322.json b/data/part_6/0885203322.json new file mode 100644 index 0000000000000000000000000000000000000000..7a918a5da04b66c0ebf41ab052156b8b90f961a0 --- /dev/null +++ b/data/part_6/0885203322.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"534803905f29e75cd82ac0c9ecdb8f3e","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/2fd4b539-8fb3-437d-91ff-93ebd9c81567/content","id":"497519225"},"keywords":[],"sieverID":"d7ce8cea-82ea-4b5c-8127-f7efc160b3e4","pagecount":"15","content":"Genomic prediction models have been commonly used in plant breeding but only in reduced datasets comprising a few hundred genotyped individuals. However, pedigree information for an entire breeding population is frequently available, as are historical data on the performance of a large number of selection candidates. The single-step method extends the genomic relationship information from genotyped individuals to pedigree information from a larger number of phenotyped individuals in order to combine relationship information on all members of the breeding population. Furthermore, genomic prediction models that incorporate genotype ´ environment interactions (G ´ E) have produced substantial increases in prediction accuracy compared with single-environment genomic prediction models. Our main objective was to show how to use single-step genomic and pedigree models to assess the prediction accuracy of 58,798 CIMMYT wheat (Triticum aestivum L.) lines evaluated in several simulated environments in Ciudad Obregon, Mexico, and to predict the grain yield performance of some of them in several sites in South Asia (India, Pakistan, and Bangladesh) using a reaction norm model that incorporated G ´ E. Another objective was to describe the statistical and computational challenges encountered when developing the pedigree and single-step models in such large datasets. Results indicate that the genomic prediction accuracy achieved by models using pedigree only, markers only, or both pedigree and markers to predict various environments in India, Pakistan, and Bangladesh is higher (0.25-0.38) than prediction accuracy of models that use only phenotypic prediction (0.20) or do not include the G ´ E term.G lobal wheat production is increasing by less than 1% annually and recently, wheat yields have stagnated in many regions of South Asia (Ray et al., 2012). In South Asia, the wheat crop is already being grown under high temperature conditions; however, because of climate change, temperatures could increase well beyond the optimal for growing wheat, which would further reducegrain yield. As a result, South Asian countries may not be able to meet the region's already growing demand for wheat grain.Well-managed crop improvement programs are necessary to increase food production in different parts of the world. Several molecular marker methods have proven their relevance in different cereal crops. Genomic selection (GS) is becoming a standard approach to achieving genetic progress in plants because it reduces the generation interval by reducing the need to have progeny field-tested every cycle. Breeding values can be predicted as the sum of the effects of all markers by regressing the values of the phenotypes on all markers (Meuwissen et al., 2001). Several authors have successfully implemented GS in plant breeding with intermediate to high density marker coverage for traits such as grain yield, biomass yield, resistance to several diseases, and flowering evaluated under different environmental conditions. Studies have demonstrated that some of the factors determining prediction accuracy in GS are the heritability of the trait, the number of markers, the size of the training population, the relationship between the training and the testing sets, and G ´ E (de los Campos et al., 2009;Crossa et al., 2010Crossa et al., , 2011;;Pérez-Rodríguez et al., 2012;Burgueño et al., 2012;Hickey et al., 2012;González-Camacho et al., 2012;Riedelsheimer et al., 2012;Weber et al., 2012). Furthermore, including highdensity marker platforms with G ´ E interactions adds power to GS models (Burgueño et al., 2012;Jarquín et al., 2014;López-Cruz et al., 2015;Heslot et al., 2012).Recently, genomic predictions have been extensively studied in bread wheat using elite germplasm sets (de los Campos et al., 2009Campos et al., , 2010;;Crossa et al., 2010;González-Camacho et al., 2012;Heslot et al., 2012;Pérez-Rodríguez et al., 2012;López-Cruz et al., 2015). The results have proven that the use of dense molecular markers coupled with pedigree information increases the prediction accuracy of unobserved phenotypes. One of the problems usually encountered by GS in animal and plant breeding is that the number of evaluated lines exceeds the number of genotyped lines, because of the genotypic costs. Nejati-Javaremi et al. (1997) were the first to propose incorporating genotypic information for predicting the breeding values of animals in a similar manner to the way pedigree information is used in the best linear unbiased predictor method. When the pedigrees of all phenotyped individuals were available but only some were genotyped, dairy cattle researchers (Misztal et al., 2009;Legarra et al., 2009;Aguilar et al., 2010Aguilar et al., , 2011;;Christensen et al., 2012) derived a unified (single-step) computation approach for Genomic Best Linear Unbiased Predictor (ssGBLUP) for combining phenotypic, pedigree, and genomic information based on Henderson's (1975Henderson's ( , 1976) ) standard mixed model equations. These authors augmented a pedigree-based relationship matrix (Matrix A) with contributions from a genomic relationship matrix (Matrix G) of the genotyped individuals. They showed how to modify the original Matrix A to obtain Matrix H, which includes not only the pedigreebased relationship matrix but also a matrix that contains the differences between genomic-based and pedigreebased matrices. These authors also developed efficient computer algorithms for inverting Matrix H computed from large numbers (millions) of animals in the data.Although augmenting Matrix A by using only a fraction of the individuals that were genotyped would reduce genotyping costs, the ssGBLUP method has not been extensively applied in plant breeding. Just recently, Ashraf et al. (2016) were the first to investigate the impact on prediction accuracy when some wheat lines were not genotyped and only pedigree and phenotype information was available; the authors concluded that the ssGBLUP method for deriving Matrix H can provide higher prediction accuracy than either genomic or pedigree-based prediction. In plants, the ssGBLUP approach proposed by Ashraf et al. (2016) has been used with a limited number of lines. The approach has not been tested on large datasets [e.g., CIMMYT's Global Wheat Program (GWP), which generates thousands of new breeding lines that are candidates for field evaluation every cropping cycle]. Applying GS in the GWP is economically feasible (i) when advancing breeding lines in the first preliminary yield trials to predict the performance of the selected lines in multienvironment trials or (ii) for predicting a selected set of lines in different international target environments using the parents evaluated in Mexico and the progeny to be predicted in international environments such as those in South Asia as a training set.In recent years, the GWP aimed to form a large reference dataset comprising 58,798 breeding lines, including the lines' phenotypic and pedigree data from the last seven cropping cycles in Ciudad Obregon, Mexico, and South Asia. This large reference set contains complete phenotypic data and pedigree information; however, only 29,484 of the lines have been genotyped. Therefore, an H matrix that combines wheat lines that have molecular markers only with those that have pedigree and phenotype must be generated.The main objectives of this study were (i) to use the large reference set for predicting the performance of wheat lines in several environments in South Asia; and (ii) to perform predictions using phenotypic, pedigree, and genomic information to evaluate the wheat lines genetically using a single-step model that combines pedigree and marker information into a unified H matrix. Here, we used information for genotyped and nongenotyped individuals combined by applying the method proposed by Legarra et al. (2009) and Aguilar et al. (2010). Prediction accuracy was studied using a G ´ E interaction multiplicative model (the reaction norm model of Jarquín et al., 2014) with pedigree information (Matrix A), genomic information (Matrix G), or both (Matrix H) and comparing its prediction accuracy results with those of a genomic model that does not include the G ´ E interaction. This reaction norm model uses highly random dimensional matrices for the genomic and pedigree matrices. We also describe the statistical and computational challenges encountered when developing the pedigree and single-step models in such large datasets.The dataset included a total of 58,798 wheat lines that were evaluated at the Norman E. Borlaug Experiment Research Station in Ciudad Obregon, Mexico, under various field management conditions (optimal, drought, late heat, severe drought, and early heat) during seven cycles (2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016). Some of the lines were also evaluated under the same conditions in South Asia (Jalbapur, Ludhiana, and Pusa in India; Faisalabad in Pakistan; and Jamalpur in Bangladesh) during 2013 to 2016. The original data from each year comprise a large number of trials, each established using an a-lattice design with three replicates. The field management conditions under which each trial was established in each year are described in Table 1. The condition-location combinations will be referred to as environments. Table 2 shows the number of lines evaluated in each environment.The basic model fitted to each of the 12 environments described in Table 2 comprises the random effects of the trials, the random effects of the replicates within trials, the random effects of the incomplete blocks within trials and replicates, and the random effects of the breeding lines.A pedigree relationship matrix (A) for the 58,798 individuals was computed using a modified version of the software 'pedigreemm' (Bates and Vazquez, 2009) that accounts for self-pollination; the latest version of the routines can be found at https://github.com/Rpedigree/ pedigreeR (accessed 5 Apr. 2017). Given the dimensions of A, it is difficult to hold it in random access memory (RAM) and compute it. Appendix A shows the small R script (R Core Team, 2016) that was used to obtain and store the relationship matrix. It uses results from partitioned matrices to obtain the results and speed up the computations; R was recompiled from the source and linked with OpenBLAS [http://www.openblas.net (accessed 5 Apr. 2017)]. For further details on the computations, see Appendix A. In total, 29,484 individuals were genotyped using genotyping-by-sequencing (e.g., Elshire et al., 2011). We kept all the single nucleotide polymorphism markers and imputed the missing values using observed data. Markers with a minor allele frequency of less than 0.05 were removed; after this process, 9045 markers were available for prediction.Recently, Jarquín et al. (2014) and López-Cruz et al. (2015) proposed statistical models for performing genomic predictions taking G ´ E into account. The models were originally developed to incorporate genetic information from molecular markers and, in the case of Jarquín's model, it is also possible to incorporate environmental covariates. Jarquín's model has also shown to be useful when the genetic information is obtained from a pedigree (Pérez-Rodríguez et al., 2015). Here, we describe Jarquín's model based on genomic and pedigree information. To speed up the computations and make them feasible, we reparametrized the original model by using very well-known results from Cholesky decomposition and mixed models (e.g., Henderson, 1976;Harville and Callanan, 1989).The parametric G ´ E interaction model takes the main effect of environments (E), the main effect of genotypes and the interaction between genotypes and the environment into account. In matrix notation, the model can be written as: ~( , ( )#( )) MN u 0 Z GZ Z Z , where # denotes the Hadamard product (cell by cell) of the two matrices in parentheses (see Jarquín et al., 2014;Pérez-Rodríguez et al., 2015). Finally, we assume that the residuals are distributed as follows:e MN e 0 I , where e is the residual error; MN is the multivariate normal, and I is the identity matrix.Since A is positive definite and symmetric, it can be factored asA LL by using Cholesky decomposition where Matrix L is a lower triangular matrix with positive diagonal entries and is usually named the Cholesky factor. Therefore, from Eq. [1]:where s * 2 1 u ~( , ) MN u 0 I . Furthermore, it is not necessary to calculate the Z g L product because for each row of the resulting matrix, we just need to copy the k th row of L, where k is the column in the i th row of Z g that is different from zero (i.e., Z g (i, k) = 1). The matrixZ Z is a block diagonal; blocks different from zero correspond to matrices with ones:) is a square matrix with ones whose dimensions correspond to the number of genotypes evaluated in environment j. Since# Z AZ Z Z , we just need to compute the corresponding block elements in the diagonal ofThe block diagonal elements of  A can be computed as follows:where , jj A corresponds to the relationship matrix for individuals evaluated in environment j. From Eq. [3] and Eq. [5] and by using Cholesky decomposition, the term# Z AZ Z Z can be obtained as follows:( ))whereTherefore, from Eq. [6], we obtain: Therefore, using the results from Eq. [2] and Eq. [7], Model 1 can be written as:Equation [1] and Eq. [8] are equivalent, but Eq. [8] has at least two advantages over Eq. [1]: (i) it avoids many matrix products and (ii) it can be implemented relatively easily using the well-known Gibbs sampler (Geman and Geman, 1984) in the Bayesian framework.Let W be a ǵ p matrix of standardized markers, where g is the number of genotyped individuals and p is the number of markers; letbe the genomic relationship matrix (López-Cruz et al., 2015). A model similar to Eq. [8] can be obtained by replacing A with G.Model 3: G ´ E Interaction Using Molecular Markers and Pedigree (Single-Step Approach)In this model, the information for genotyped and nongenotyped individuals is combined using the approach proposed by Legarra et al. (2009) and Aguilar et al. (2010). A relationship matrix that includes full pedigree and genomic information is given as:where the matrix is divided according to whether the individuals have been genotyped or not. Submatrices gg nn , A A , and gn A are submatrices of A containing the relationships among genotyped individuals, among nongenotyped individuals and between genotyped and nongenotyped individuals, respectively (Legarra et al., 2009;Christensen et al., 2012). G a is an adjusted relationship matrix obtained from the genomic relationship matrix given by López-where b and a are obtained by solving the following system of equations:where G a is a rescaled matrix such that: (i) the average of the diagonal elements is equal to the average of the diagonal elements of A gg , and (ii) the average of all the elements is equal to the average elements of A gg . See Christensen et al. (2012) for further details. Note that in this formulation based on H (and not its inverse), H does not need to be full rank.The Appendix shows the R code that allowed us to build Matrix H. A parametric G ´ E interaction model takes the effect of the environments, the main effect of genotypes and the G ´ E interaction into account. A model that uses information obtained from markers and pedigree can be obtained by replacing the A matrix in Model 1 with the Matrix H described above (Eq. [8]).Note that models that do not include the G ´ E term can be derived from Model 1 to Model 3 just by removing the corresponding random G ´ E term. For example, by removing the term u 2 representing the effect of G ´ E from Model 1 (Eq. [8]), it becomesIn this case, the resulting models are equivalent to the cross-environment genomic best linear unbiased predictor model of López-Cruz et al. (2015). We include models without the G ´ E term to compare the prediction accuracy of models with and without G ´ E interactions. The singleenvironment model was not included because all the wheat lines included in the prediction of South Asian environments had complete pedigree and markers, and thus developing Matrix H for the single-step model did not make sense.The main interest of breeders is to predict the performance of nonevaluated lines in South Asian sites (Jalbapur, Ludhiana, and Pusa in India; Faisalabad in Pakistan; and Jamalpur in Bangladesh). To mimic that situation, we designed a cross-validation scheme where we fitted the G ´ E models (Models 1-3) as well as models without G ´ E using all available records under drought, late heat, optimal, and severe drought conditions obtained in Ciudad Obregon (Mexico), and 20% of available records in each of the South Asian sites assigned at random as the training set. In the prediction process, 80% of lines in the corresponding sites in the South Asian countries (India, Pakistan, and Bangladesh) were predicted using the rest of the records. A total of 20 random partitions (such as the ones described above) were generated.The models' predictive abilities were compared by using Pearson's correlation coefficient. The models that used the A and H matrices included the phenotypic information of the 58,798 wheat lines, whereas the model that was based on markers only included information for 29,484 wheat lines that correspond to the individuals that were genotyped. The genotyped individuals were a subset of the individuals with pedigree information; therefore, lines in the testing set had pedigree and marker information. The numbers of individuals in the testing sets in South Asian sites were shown in Table 3, so in each random partition, the same individuals are predicted with three different models based on the A, G, and H matrices.The models described above were fitted using a modified version of the BGLR package (de los Campos and Pérez-Rodríguez, 2015). The package was modified to accept big.matrix objects created using the bigmemory package as input (Kane et al., 2013). The bigmemory package was used to handle the huge matrices that had to be used during the analysis and also to take advantage of what in computer science is known as \"shared memory\". Once loaded into RAM memory, the data can be accessed from several processors, making it possible to perform a crossvalidation relatively easily.Figure 1 shows a boxplot of grain yield per location and median yield per location. From the plot, it can be seen that the optimal conditions had the highest grain yield, whereas the late heat and severe drought conditions had the worst grain yield. Yields in South Asian environments, especially in Pakistan and Bangladesh, were usually lower than those in Mexican environments. Table 4 shows the number of lines evaluated in each environment and the number of lines in common between pairs of environments. It also shows sample correlations for grain yield for each pair of environments. The number of lines evaluated in common between pairs of environments ranged from 537 to 4735. The phenotypic sample correlation ranged from -0.05 to 0.53, which suggests large G ´ E effects.Figure 2 displays the distribution of the diagonal entries for Matrices A, H, and G. Note that in the A matrix, the diagonal entries are around ~1.5; in this case, ( ), where i F is the inbreeding coefficient of the i th individual. The diagonal entries of Matrix G are around 1.0, reflecting the fact that the markers were centered and standardized. The diagonal entries of Matrix H are around 1.5, which stems from standarding G to be on the same scale as A.Table 4 shows the phenotypic correlations between pairs of environments. For example, the phenotypic correlation of the 4062 wheat lines in common between the environment with the bed planting with five irrigations at Obregon and the bed planting with two irrigations at Obregon is 0.156, whereas the phenotypic correlation of the 1537 wheat lines in common between the bed planting with five irrigations at Obregon and standard management conditions at Pusa, India, is 0.210. In general, the phenotypic correlations were not high, ranging from -0.051 to 0.481. Table 5 shows the average Pearson's correlations between observed and predicted phenotypes and their corresponding SD for the model without G ´ E. The average correlations come from 20 random partitions with all the data records available in Mexico and 20% of the data available in South Asia. Note that these are the predictions for 80% of the entries included in the six South Asian environments. The prediction accuracies are relatively low, with those based on pedigree being slightly higher than those based on markers or on both pedigree and markers.Table 6 shows the average Pearson's correlations between the observed and predicted phenotypes and the corresponding standard obtained using the same cross-validation scheme described above but including the G ´ E Table 5. Correlations (plus SD in parentheses) between predicted and observed values obtained by using the cross-validation where all the wheat lines from Ciudad Obregon, Mexico, plus 20% of the wheat lines in each of the environments in India, Pakistan, and Bangladesh were used in the training set to predict 80% of the lines in the corresponding environments in India, Pakistan, and Bangladesh for models without genotype ´ environment effects (G ´ E). term. The predictive ability of models based on pedigree, markers, and pedigree + markers is about the same, with pedigree prediction accuracy being higher than genomic and pedigree + genomic prediction accuracy in four environments (delayed planting at Faisalabad, standard management at Faisalabad, standard management at Jamalpur, and standard conditions at Pusa). Ludhiana and Faisalabad under standard management conditions (0.3785, 0.2455, respectively) were the best predictive models for the genomic and pedigree + genomic model, respectively.Figure 3a-c shows scatterplots of the predictive correlations for each of the 20 cross-validations across the six environments in South Asia. Figure 3a depicts the correlations between predicted values based on markers (Matrix G) versus those based on Matrix H and shows that the prediction accuracy based on Matrix G was superior to that obtained based on H. Figure 3b displays the correlation based on markers (Matrix G) versus that obtained based on pedigree (Matrix A), where the prediction based on pedigree seems slightly better than that based on Matrix H (Fig. 3c).Table 7 shows the percentage of change in the prediction accuracy of models with and without G ´ E. The percentage of change was calculated as:where G×E r is the Pearson's correlation for a model with the G ´ E term and no G×E r is the Pearson's correlation for a model without the G ´ E term. From the results in Table 7, it is clear that models that include the G ´ E term predict better than those that do not include G ´ E. For example, the G ´ E model using Matrix H gave a 66% increase in prediction accuracy compared with the model using Matrix H but without G ´ E.Figure 4 presents a bar plot of correlations for each predicted environment in South Asia using the H matrix. Black bars represent the mean of the weighted phenotypic correlation of a given environment and the rest of the environments in Table 4. The phenotypic correlation for environment j in South Asia can be obtained as follows:where = ¼ 1, ,6 j (environments in South Asia) andrepresents the set of environments in South Asia and Mexico excluding environment j, n jk corresponds to the number of lines in common between environments j and k, = å j jk k n n , and r jk is the phenotypic correlation between environments j and k. As an example, Table 8 presents the information needed to compute the weighted correlation for the environment with delayed planting at Faisalabad; the columns present the information needed to compute the weighted correlation (note that this information was obtained from Table 4). The rest of the correlations were obtained by using the approach described above. The gray bars represent the means of the correlations between the observed and predicted values obtained from cross-validations. Note that in general, the G ´ E models gave good predictions, usually better than those from the phenotypic correlations.Although we predicted 80% of the records in each of the South Asian environments, the correlations are higher than the phenotypical correlations between a given environment and the rest of the environments.In wheat breeding, the cost of genotyping thousands of plants in segregating populations or in advanced generations makes the application of GS unfeasible. One possibility for solving this problem would be to augment the numerical relationship Matrix (A) of all individuals with the genomic relationship matrix (G) of the genotyped individuals and to perform predictions based on the resulting complete Matrix H, which would allow us to predict nongenotyped individuals in the testing set. Augmenting Matrix A by using only a fraction of the genotyped individuals would reduce genotyping costs. Furthermore, as described by Christensen et al. (2012), the single-step method allows the genomic relationship matrix of genotyped individuals to be extended using pedigree information to a combined relationship Matrix H of all individual plants or lines. This allows one to use all phenotypic data and not merely data from phenotypes that have pedigree and marker information; this extra phenotypic information should also enhance prediction Table 7. Comparison of the predictive ability of models with and without genotype ´ environment effects (G ´ E).-----------% change † ------- 6 using Matrix H. Black bars represent the weighted mean of the phenotypic correlation of a given environment and the rest of environments in Table 4; for example, for DPL_FAS, the weighted correlation can be obtained by using the data shown in Table 8. FAS, Faisalabad, Pakistan; JAM, Jamalpur, Bangladesh; JBL, Jabalpur, India; LDH, Ludhiana, India; OBR, Obregon, Mexico; PUS, Pusa, India; STN, standard management conditions; DLP, delayed planting conditions.accuracy. This makes the models and methods developed by Misztal et al. (2009), Legarra et al. (2009) and Aguilar et al. (2010;2011) very attractive for predicting unobserved and nongenotyped plants.In a recent article, Fernando et al. (2014) proposed a single-step Bayesian regression strategy that allows the use of all genotyped and nongenotyped individuals by means of imputed marker covariates for nongenotyped individuals. The advantage of the Bayesian approach over the single-step best linear unbiased predictor is that it does not require one to compute the inverse of G. However, this model has not yet been applied to realistic datasets.The single-step approach of Misztal et al. (2009), Legarra et al. (2009) and Aguilar et al. (2010;2011) was used in dairy cattle studies and first applied to plant breeding data by Ashraf et al. (2016) in a set of 1176 genotyped CIMMYT wheat lines and 11,131 nongenotyped wheat lines tested in five environments in Ciudad Obregon, Mexico, during the 2012-2013 cycle. We developed optimized weighting factors for Matrix H and applied a multivariate method for assessing G ´ E and found that the prediction accuracy of the single-step H matrix was higher than the accuracies achieved using the A and G matrices. The present study used seven selection cycles of CIMMYT wheat breeding with a total of 58,798 wheat lines evaluated in Ciudad Obregon and predicted several wheat lines in South Asian environments (India, Pakistan, and Bangladesh).From the results in Table 5 to Table 7, it is clear that models that include the G ´ E term predict the environments in South Asia better than models that do not include the G ´ E term. The gain in the prediction accuracy of models that include G ´ E ranges from 16 to 90% with an average of 40%. However, models that do not incorporate G ´ E but use pedigree or high-density molecular markers, or both are still superior in terms of prediction accuracy than those that use phenotypic data only.In this study, we assessed the prediction accuracy of a large number of wheat lines evaluated in several environments and years in Ciudad Obregon, Mexico, and predicted lines in several South Asian environments. For Ludhiana, Pusa, and Jabalpur, about 1227 wheat lines were predicted on the basis of the performance of these lines in six environments in Ciudad Obregon plus the performance of about 57,000 wheat lines related to those to be predicted (1227) and evaluated in previous years in Ciudad Obregon, Mexico.Prediction accuracy was the correlation between the predicted values of the lines in Ciudad Obregon plus a low proportion of them (20%) in six environments in South Asia using three G ´ E models (those using Matrices A, G, and H) with the observed values of 80% of the lines in the six environments in South Asia (which were not phenotyped). The correlations for all the environments were around 0.25 to 0.27, except for Ludhiana in India, which showed higher prediction accuracy (0.36-0.37). These genomic prediction accuracies were higher than the prediction accuracies computed from the common phenotypic correlations between all pairs of environments. These results indicated that the prediction accuracy with which breeders make selections in Ciudad Obregon, Mexico, is lower than the accuracy they could obtain by performing genomic selection and prediction. Although wheat breeders expect that lines selected in Ciudad Obregon will perform well in South Asian environments, the results of this study should prompt them to increase research on genomic selection in Ciudad Obregon (a very stable site with high radiation) of candidates for selection that will perform well in several environments in different South Asian countries (India, Pakistan, and Bangladesh).The prediction accuracy of models with Matrices A, G, and H for models with or without G ´ E did not change much. This is an important result that allows, through the use of Matrix H, one to use all phenotypic data to predict the genetic values of the unobserved wheat lines, thereby avoiding having to use only a subset of the phenotypes of lines with pedigree data n 1 = 15,448 r 1 = 0.18 † k = 1,…,11 represents the environments, r 1 represents the weighted phenotypic correlation for Environment 1 in South Asia (i.e. Faisalabad), and n 1 is the total of the column labeled as n jk . ‡ FAS, Faisalabad, Pakistan; JAM, Jamalpur, Bangladesh; JBL, Jabalpur, India; LDH, Ludhiana, India; PUS, Pusa, India; STN, standard management conditions; DLP, delayed planting; B5I, bed planting and five irrigations; B2I, bed planting and two irrigations; MEL, Melgas flat planting; DRB, bed planting and drip irrigation; EHT, early heat; LHT, late heat and another subset of phenotype data from lines with marker data only. The single-step method for computing Matrix H allows the inclusion of both components of the breeding value to be predicted: the parental average or between-family variability captured by the pedigree and the Mendelian sample component (or with family variability) accounted for the by markers.Big Data Used to Derive Pedigree and Combine it with Markers into the Single-Step Prediction Method with a G ´ E Model So far, no studies using plant breeding data on more than 50,000 lines have been reported in the GS literature. This is the first study to show that large training populations can provide genomic predictions that are more precise than phenotypic predictions. This is the first time that the theory used to develop and implement the pedigree system for such a large number of lines has been reported in plant breeding. Although the models used for prediction are now well known, from the computational and statistical points of view, it is necessary to develop algorithms and data structures that allow researchers to handle the data and fit the models efficiently.In this study, we used the G ´ E reaction norm model on a large dataset in conjunction with pedigree, markers, or both, in GS and prediction. We compared models including and excluding G ´ E. In the genomic prediction literature, there are plenty of examples where including those interactions significantly improved the prediction accuracy of untested individuals. The single-step method that combines the use of pedigree and markers through Matrix H allows the use of all the available information. Also, the reaction norm G ´ E model allows us to swap information among positively correlated environments, although the predictive power of the model was similar to that of the model that included markers only. Ashraf et al. (2016) used the single-step H approach on a set of 11,131 nongenotyped and 1176 genotyped wheat lines.Animal breeders make extensive use of the fact that the relationship Matrix A has a very sparse inverse that can be computed directly from the pedigree, if all individuals (including those with no phenotype) are included (Henderson, 1976(Henderson, , 1977)). This results in a sparse -1 H structure as well (Aguilar et al., 2010;Christensen and Lund, 2010), with a storage cost that is quadratic in the number of genotyped individuals but is only linear in the number of nongenotyped individuals. These sparse inverses exist for any level of autopolyploid species (Kerr et al., 2012) and could potentially be used for prediction with large data sets. However, this would preclude the use of Cholesky decomposition as used in Eq. [8].This study shows how to solve statistical and computational challenges when incorporating and combining high-dimensional pedigree and genomic matrices into a single-step model for predicting unobserved individuals in other environments. We found that the genomic prediction of genotyped and nongenotyped wheat lines produces higher prediction accuracy than that of lines predicted from phenotypic data. The results provide evidence that the single-step approach that combines pedigree and marker information is useful for reducing genotyping costs while maintaining the prediction accuracy of unobserved individuals at relatively intermediate levels. The incorporation of G ´ E models using a combination of pedigree and genomic information is another way of increasing the prediction accuracy of unobserved candidates for selection and offers plant breeders an important alternative for predicting germplasm evaluated under different environmental conditions.This script computes relationship Matrix A. Inputs:(1) A text file with pedigree information for the individuals that we are interested in. The file should have three columns separated by tabs, ID (the identification number of the individual), Sire (male parent), and Dam (female parent).(2) A text file with the individuals that we are interested in.Output: The relationship matrix.To speed up the computations, we used dense partitioned matrixes and linked R with OpenBLAS (http://www. openblas.net, accessed 5 Apr. 2017). At the end of the process, the relationship matrix was also stored as a partitioned matrix on hard disk in binary R format (RData). Below, we detail the steps used to build the matrix.Step colnames(a)=c(\"Mparent\",\"FParent\",\"ID\") a=a[!duplicated(a),] cat(\"nrow=\",nrow(a),\"\\n\") cat(\"selfing=\",sum(a[,1]==a[,2]),\"\\n\") #Read the ids of individuals with phenotypic records ids=scan(\"GIDsForUSAIDprediction_20160406.csv\") ids=as.character(ids) pede=editPed(sire=a$MParent,dam=a$FParent,label=a$I D,verbose=TRUE) ped=with(pede, pedigree(label=label, sire=sire, dam=dam))Now use the relfactor function for the pedigree, that is:where X full is an upper triangular, sparse (right) Cholesky factor of the relationship matrix. In this case, X full is a matrix with n = 177,376 rows and the same number of columns. The code for obtaining the relfactor is given below.Xfull=relfactor(ped)We do not need A full ; we just need a subset of this matrix with the 58,798 individuals so we can take a subset of 58,798 columns from X full . The columns correspond to the individuals that we are interested in. Let X be the resulting matrix; we then have = A X'X , where X has n = 177,376 rows and p = 58,798 columns. The R code for obtaining this matrix is shown below.Step 2: Partition the Relationship Factor Since X is a huge matrix, it is very difficult to obtain A directly; furthermore, since X is sparse, the product cannot be parallelized easily. We then partitioned X into several submatrices and saved the submatrices as binary files that can later be retrieved in order to obtain the product. For example: where X ij is a submatrix obtained from X.The R code below was used to partition matrix X into five submatrices and save the results to binary files. ) cat(\"Submatrix: \",i,\" \",j,\"\\n\"); cat(\"from_row: \",from_row,\"\\n\"); cat(\"to_row: \",to_row,\"\\n\"); cat(\"from_column: \",from_column,\"\\n\"); #Conventional matrix object so that we can use #optimized dense matrix products Xij=as.matrix(X[from_row:to_row,from_column:to_ column]) save(Xij,file=paste(\"X_\",i,j,\".RData\",sep=\"\")) } }Step 3: Compute the Relationship Matrix using the Partitioned Matrices from Step 2 Given the partition of the relationship factor, we can compute the Matrix A as follows:where: A X X X X X X X X X X ,[A4]Note that now we need to calculate several products of matrices. There are optimized libraries that can be used for this task. For example, in R, we can recompile the program so that we can use OpenBLAS. Details are given at http://www.openblas.net/ (accessed 5 Apr. 2017) and http://www.rochester.edu/college/psc/thestarlab/help/ moreclus/BLAS.pdf (accessed 5 Apr. 2017). We recompiled R version 3.2.3 (R Core Team, 2016) in order to use OpenBLAS so it can perform matrix operations in parallel. The next fragment of code obtains Matrix A 11 using the partitioned matrices. rm(list=ls()) n_submatrix=5 A11=matrix(0,nrow=25000,ncol=25000) for(i in 1:n_submatrix) { cat(\"i=\",i,\"\\n\") load(paste(\"X_\",i,\"1.RData\",sep=\"\")) A11=A11+crossprod(Xij); } save(A11,file=\"A11.RData\")The rest of the matrices can be obtained similarly. With this approach and by using eight cores for the matrix product, we obtained the 58,798 ´ 58,798 Matrix A in less than 3 hr in the CIMMYT-BSU server, which has 12 Intel Xeon Cores (Intel, Santa Clara, CA) @ 3.47 GHz and ~ 48 Gb of RAM.The script presented below computes the relationship Matrix H that includes full pedigree and genomic information (see equation 4 in Legarra et al., 2009). It adjusts the elements of genomic relationship Matrix G, so that the entries of the relationship Matrix A share the same scale (Christensen et al., 2012). Inputs: 1) Matrices A and G. The row and column names of both matrices include the identification numbers of the individuals.Output:1) Matrix H. ","tokenCount":"6130"} \ No newline at end of file diff --git a/data/part_6/0892536308.json b/data/part_6/0892536308.json new file mode 100644 index 0000000000000000000000000000000000000000..034453378a7bbb2ce5de7e81f2fdbe72f88e2733 --- /dev/null +++ b/data/part_6/0892536308.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"2db2ec55b3cc37325e299c721533098a","source":"gardian_index","url":"http://ciat-library.ciat.cgiar.org/Articulos_ciat/ipm/pdfs/phytoplasmas_oil_palm_alvarez.pdf","id":"-1166366490"},"keywords":[],"sieverID":"a470f21e-498f-4f4d-b9b3-1b62a5827409","pagecount":"1","content":"The specific primers R16mF2/R16mR1 and R16F2n/R16R2 were successfully used in a nested-PCR assay (Figure 2) to detect and confirm that phytoplasmas were associated with 'marchitez letal'.Phytoplasmas were detected in 'marchitez letal' infected oil palm meristems, inflorescences and leaf bases.Staining methods. The presence of phytoplasmas in meristem, inflorescence, and leaf tissue was confirmed by DAPI and Dienes' stain (Figures 3 and 4). 'Marchitez letal', caused by an unidentified microorganism, is a major disease affecting oil palm and has been observed with increasing frequency in Colombia. Incidences of up to 30% have been recorded in several commercial fields in production areas of Villanueva and Casanare. Its symptoms are similar to those caused by infection with phytoplasma and include leaf discoloration, lower leaves will turn brown and hang downwards like a collapsed umbrella (Figure 1).PCR is a very sensitive method for phytoplasma detection, identification and classification.On the basis of DNA sequences, the oil palm phytoplasma was classified as a member of the 16 SrI in the aster yellows group. This is the first report of a phytoplasma in oil palm in Colombia.Insects associated with 'marchitez letal' were identified as Cicadellidae and Membrasidae.Transmission from diseased oil palm to either healthy oil palm or Catharanthus roseus was achieved by grafting.. Plant tissue. Leaf bases, inflorescences, and meristem tissue from infected and healthy oil palm plants were used in this study. Insect survey. Leafhoppers were collected from the leaves of palms affected by 'marchitez letal'. The presence of the phytoplasma was verified by nested PCR. Staining methods. Two staining methods were used as follows: DAPI, which stains the phloem (Sinclair et al., 1989), and Dienes' stain, which metabolizes and produces a blue color (Deeley et al., 1979). DNA extraction. Total DNA was extracted as described by Gilbertson and Dellaporta (1983). Nested PCR analysis. The primer pairs P1/P7 or R16mF2/R16mR1 were used for the first amplification, with an annealing temperature of 55°C. For the nested PCR, diluted PCR products were used for amplification, with the primer pair R16F2n/R16R2, at an annealing temperature of 50°C. PCR products were analyzed by electrophoresis on 1.2% agarose gel. The occurrence of what seems to be a phytoplasma different from that occurring in coconut and other palm species has been recorded in an oil palm plantation in the West New Britain area of Papua New Guinea (1) , where electron microscopy has played an important role in detecting phytoplasmas in infected tissue (Figure 1b), and in Kerala, India (2) .1. Jones, P. and Turner, P.D. 1979. The occurrence of mycoplasma-like organisms in oil palm (Elaeis guineensis). West New Britain (Papua New Guinea). 2. Babu and Nair. 1993. Distribution of spear rot disease of oil palm and its possible association with MLO disease of palms in Kerala, India. RFLP analyses. The amplified PCR products were digested with the restriction endonucleases MseI and AluI. The restriction products were analysed by electrophoresis on 5% polyacrylamide gel.Cloning and sequencing of 16S rRNA and tRNA genes. The amplified fragment was cloned and sequenced with primer T7 and Sp6.Transmission. Transmission was performed by grafting.Control treatment. Oxytetracycline antibiotic was applied to oil palm by liquid injection into the trunk (5.0 g per palm) over a 4month period.Insects found associated with oil palm in Colombia, in areas where 'marchitez letal' was present.: Grafting RFLP analysis. Restriction digestion with MseI and AluI. of the amplified product showed similar restriction patterns. Sequence analysis of the 16S rRNA genes revealed that the oil palm phytoplasma was similar to the aster yellows group, with a sequence homology of 99% regarding phytoplasma from oil rape seed (GenBank accession no. U89378). ","tokenCount":"595"} \ No newline at end of file diff --git a/data/part_6/0898359378.json b/data/part_6/0898359378.json new file mode 100644 index 0000000000000000000000000000000000000000..e06a4a09e7fe3573e5330614746618c479687818 --- /dev/null +++ b/data/part_6/0898359378.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"887dfb94b2fd87834d5733380e1084c5","source":"gardian_index","url":"https://publications.iwmi.org/pdf/H_2352.pdf","id":"1921072333"},"keywords":[],"sieverID":"19c78806-680a-47b6-9a41-948abbcc7cbc","pagecount":"26","content":"Agronomic p r a c t i c e s f o r i r r i g a t e d d i v e r s i f i e d c r o p s . T i m i n g o f c u l t i v a t i o n f o r d i v e r s i f i e d c r o p s i s p a r t i c u l a r l y i m p o r t a n t . I n a r e a s where t h e r e i s d r y s e a s o n r a i n f a l l , most d i v e r s i f i e d c r o p s c a n b e g r o w n i n e x p e n s i v e l y w i t h o u t i r r i g a t i o n . Two m a j o r p r o b l e m s r e m a i n unanswered: how t o i r r i g a t e w i t h o u t s a t u r a t i n g t h e s o i l a r o u n d t h e c r o p s , a n d how b e s t t o c o n v e r t t h e s o i l f r o m p u d d l e d c o n d i t i o n i n t h e w e t s e a s o n when r i c e i s g r o w n , t o a w e l l -s t r u c t u r e d a n d a e r a t e d c t , n d i t i o n f o r d i v e r s i f i e d c r o p s i n t h e d r y s e a s o n , and v i c e v e r s a . 9 . Economic a n d i n s t i t u t i o n a l a s p e c t s o f i r r i g a t i n g d i v e r s i f i e d c r o p s . U n s t a b l e p r i c e s a n d h i g h i n p u t c o s t s o f n o n -r i c e c r o p s d i s c o u r a g e f a r m e r s f r o m a d o p t i n g i r r i g a t e d d i v e r s i f i e c . c r o p p i n g . E x p e c t a t i o n s o f p r o f i t a b i l i t y is one o f t h e f o r e m o s t c o n s i d e r a t i c , n s o f f a r m e r s i n m a k i n g t h e i r c r o p p i n g d e c i s i o n s .The IRRI-IFPRI Studylon f,od demand and supply for Developing Member Countries, using the Philippines a s a case study, concluded that the Philippines has comparative advantiige in producing both irrigated rice and irrigated diversified (non-rice) cr'ops. A second phase of the study i s now underway t o further refine strateg:.es for agricultural development for the Philippines and to assist in the formulation of plans to attain optimum productivity in rice, corn and othor crops in the different regions of the country. The analysis will includf! the development of regional agricultural development strategies, estimating levels of investment for alternative irrigation options, assessing the possibilities for crop diversification, and recommending appropriate agricultural and irrlgatlon policies for the country.1 The International Rice Resetrch Institute (IRRI) and the International Food Policy Research Institute (IFI'RI) jointly conducted a regional survey o f 20 developing member countries. Tile survey, financed by the Asian Development Bank (ADB), was entitled \"Study on Food Demand and Supply and Related Strategies for DMCs: Regi')nal Technical Assistance No. 5116, Phase I -2 -2 .A n important aspect of tho second IFPRI-IRRI study i s to assess the technical and socio-economic constraints to irrigated crop diversification. A Technical Assistance ( T A 654-PHI:t was granted by the Asian Development Bank (ADE) to the Government of the Repiiblic of the Philippines for this purpose.This TR entitled \"Study on 1rrigat:on Hanagement for Crop Diversification\" is being implemented by the International Irrigation Hanagement Institute (IIHI) since February 1985. The IIHI study is designed t o provide detailed information about the prospects of diversified cropping under irrigated conditions.The IINI study (hereafter referred to as the Phase I Study) examined: (1) constraints to crop diversification, with special attention t o the irrigation constraint; ( 2 ) way!; in which the management of irrigation systems, particularly their operat:.on and maintenance ( O h H ) , can overcome these constraints and thereby promote crop diversification; ( 3 ) preliminary agronomic and economic comparisons of different irrigation management alternatives with various crops; ( 4 ) assessment of ObH institution-building requirements resulting from crop d:.versification, and ( 5 ) required follow-up actions. The results from the Second Phase IFPRI-IRRI s';udy are not yet available in published form, but the preliminary findings have not supported the earlier conclusions which strongly supported irrigated crop diversification in the Philippines.In short, the prospects for efficient and profitable production of irrigated crops other than rice remains highly questionable in the Philippines. Technical (in respec': of both agricultural and irrigation technologies), economic, and insti'xtional factors affecting the performance of irrigated non-rice crops are no'; yet adequately understood to permit definitive assessment of future cropping trends. The Phase I Study utilized field studies to examine the more important of these issues i n depth.Results and Assessments o f the 11\" Phase I Study 6.Irrigation water manageme&.The study showed that to effectively irrigate diversified crops, large 'canal capacities a r e needed, particularly in areas of sandy soils. Existing rice gravity systems designed for rice can accommodate these large and intermittent demands by extending the duration of water delivery periods, but only if appropriate control of water deliveries i s maintained.On-farm irrigation facilities built for rice require modifications to provide the proper water regime for diversified crops. Continuous flows of irrigation, the norm for rice, result In water-logged conditions which harm the prospects of non-rice cro3s. Irrigation of diversified crops require scheduled water deliveries on an intermittent basis and an intensive system of field channels and drains. On-farm irrigation methods involve basin flooding in some cases and furrow irrigation in others.e n s u r e t h a t t h e s t u d y c o n t r i b u t e t c t h e l a r g e r g o a l s o f a g r i c u l t u r a l p r o d u c t i v i t y i n i r r i g a t e d a r e a s o f t h e P h i l i p p i n e s , t h e Committee recommended t h a t p r i o r i t y b e g i v e n t o t h e e x t e r s i o n o f s t u d i e s o n (1) managing t h e m a i n and d i s t r i b u t i o n n e t w o r k o f i r r i g a t i o n s y s t e m s , ( 2 ) o n -f a r m i r r i g a t i o n methods and f a c i l i t i e s , ( 3 ) a g r o n o f l i c p r a c t i o e s , a n d ( 4 ) e c o n o m i c a n d i n s t i t u t i o n a l a s p e c t s o f i r r i g a t e d c r o p d i v e r s i f i c a t i o n . D u r i n g t h e p a s t t w e n t y y e A r s t e c h n o l o g i c a l change has r e s u l t e d i n a g r a d u a l i n c r e a s e i n t h e v a l u e of i r r i g a t i o n i n t h e d r y s e a s o n . The m a i n r e a s o n f o r t h i s s h i f t i s t h e s d o p t i o n o f modern r i c e v a r i e t i e s whose y i e l d p o t e n t i a l i s much h i g h e r i n t h e dr.1 s e a s o n t h a n i n t h e w e t .The eCOnOmiC v i a b i l i t y o f f a r m i n g and o f i n v e s t m e n t s i n i r r i g a t i o n s y s t e m s 1 s i n C r 0 a S l n g l y d e p e n d e n t upon d r y -s e a s o n c u l t i v a t i o n .-5 -2 0 .C o m p e t i t i o n f o r the l i m i t o d s u p p l y o f d r y -s e a s o n w a t e r has i n c r e a s e d g r e a t l y a s a r e s u l t . Because i t t i i k e s a l m o s t t w i c e a s much w a t e r p e r ha t o grow r i c e i n t h e d r y s e a s o n a s i n t h e w e t , t h e s y s t e m s do n o t have a d e q u a t e c a n a l c a p a c i t i e s t o d e l i v e r t h e f u l l d r y -s e a s o n w a t e r r e q u i r e m e n t f o r r i c e e v e n t o t h e l i m i t e d a r e a s p l a n t e d i n t h e d r y season.Head-end f a r m e r s t a k e w h a t e v e r measures t h e y c a n t o a p p r c s p r i a t e inore w a t e r t o t h e i r f i e l d s .T h i s s y s t e m o f i r r i g a t i o n i s c h a r a c t e r i ~e d b y d i s o r d e r , i n e f f i c i e n c y a n d i n e q u i t y . o n c e n t r a t e d . The r e s u l t i s t h a t f a r m e r s a t t e m p t t o grow r i c e on t h e a r e a s l e a s t w e l l s u i t e d f o r t h a t c r o p . l h e s e a r e a s r e q u i r e much l a r g e r r a t e s o f w a t e r s u p p l y t h a n t h e s y s t e m i s deriigned t o c a r r y b e c a u s e o f t h e h i g h seepage l o s s e s f r o m s u c h l i g h t s o i l s .I t tlas been e s t i m a t e d t h a t some i r r i g a t i o n s y s t e m s s u p p l y c v e r 60 p e r c e n t o f t h e i r t o t a l w a t e r t o o n l y 1 5 p e r c e n t o f t h e i r commands f o r t h i s r e a s o n . Develop on-farm irrigatior methods for at least one upland crop;( 5 )Design and field-test operating procedures for publicly-managedRecommend those policies likely to support more profitable farming practices and more profitable investment in irrigation development as related to diversified crops.It is important to be clear what the study does not propose to do. It is not proposed to undertake varietal or agronomic trials o f crops, nor to compare different diversified crop: with the objective of finding optimum crops or cultivation practices. It is not proposed to undertake macroeconomic analyses of market prospects for any crops. The study is not designed to carry out research on irrigation structures at either the system or on-farm levels. It i s the objective of the proposal to develop and fieldtest practices which will make divfrsified cropping with irrigation more profitable, but it is Government's prerogative if it uishes, not IIMI's objective, to press rice-growing farmers to adopt them. On Mindanao Island:( 1 )Allah River Irrigation Prcject (CIRIP),( 2 )Eanga River Irrigation Syztem ( E A R I S ) , and( 3 )Mani River Communal Irrigrtion System (MCIS);On Luzon Island:(1). Bustos-Pandi Pump Irrigation System (BPIS) or a similar pump system in Bulacan,( 2 ) .Laoag-Vintar River Irrigabion System (LVRIS),(3).Upper Talavera River Irri(1ation System (Upper TRIS), and( 4 ) .2 1 .These systems provide a r;.nge of climatic and soil conditions representative of the two most inporti.nt irrigated regions o f the Philippines. Their selection was based on many factors including the availability of N I A field and counterpart staff who will assist i n carrying out the studies. (1).Determine f o r one system 1.n Mindanao and one i n Luzon t h o s e limited a r e a s f o r which s e l e c t e d t l i v e r s i f i e d c r o p s a r e p a r t i c u l a r l y well s u i t e d , t a k i n g i n t o accouiit t h e n a t u r e of t h e s o i l s , t o p o g r a p h y , d i s t r i b u t i o n system, r a i n \" a l 1 , and o t h e r r e l e v a n t f a c t o r s . From t h i s i n f o r m a t i o n a more generalized methodology f o r i d e n t i f y i n g such a r e a s will be developed and f i e l d t e s t e d on one o r more a d d i t i o n a l s y s t e m s ; For two o f t h e systems, the r e s u l t s will be f u r t h e r a n a l y z e d a c c o r d i n g t o d i f f e r e n t a s n u a p t i o n s o r d a t a on t h e management of i r r i g a t i o n s u p p l i e s ;( 3 ) .t h e i r l a n d t o p r e p a r e f o r wet s e a s o n r i c e a g a i n , g i v i n g s p e c i a l a t t e n t i o n t o l a b o r and poi,rer r e q u i r e m e n t s f o r t i l l a g e , t i m e l i n e s s , m o i s t u r e r e g i m e s , p r o v i s i o n f o r f i e l d c h a n n e l s , and o t h e r r e l e v a n t f a c t o r s ;( 4 ) .[ A ] . Determine and f i e l d -t e s t a p p r o p r i a t e c o s t -e f f e c t i v e i r r i g a t i o n methods a t b o t h t h e f i e l d l e v e l and system l e v e l i n one system i n Nindanao and one i n Luzon, t o f i n d p r a c t i c a l v a l u e s f o r recommended ( a ) i n t e r v a l s between, ( b ) d u r a t i o n , and ( c ) s t r e a m s i z e o f i r r i g a t i o n s . The f i e l d -h v e l s t u d i e s will g i v e s p e c i a l a t t e n t i o n t o r e l a t e between t h e on-far'n and main-system p r a c t i c e s . The s y s t e m sl e v e l s t u d i e s will g i v e s ' i e c i a l a t t e n t i o n t o c o s t -e f f e c t i v e and manageable means of p r o v i j i n g i r r i g a t i o n on an i n t e r m i t t e n t b a s i s , keeping i n m i n d t h e need t o i r r i g a t e both r i c e and d i v e r s i f i e d c r o p s from t h e same s y s t e m ; -8 -[ B ] . Document and a n a l y z e c u r r e n t methods i n u s e d u r i n g t h e d r y s e a s o n f o r i r r i g a t i n g divv!rsified c r o p s i n t h e f i v e o t h e r sample systems, a n d a n a l y z e them f o r more g e n e r a l a p p l i c a b i l i t y ; and( 5 ) .Recommend a p p r o p r i a t e i r r : . g a t i o n management p r a c t i c e s from t h e a b o v e , g i v i n g s p e c i a l a t t o n t i o n t o a s s o c i a t e d i n s t i t u t i o n a l and management a r r a n g e m e n t s i n c l u d i n g ( a ) s t r u c t u r e of i r r i g a t i o n s e r v i c e f e e c h a r g e s , ( b ) !;ystem s t a f f i n g p l a n a n d staff t r a i n i n g , ( c ) i r r i g a t i o n a s s o c i a t i o n s and t h e i r r e l a t i o n t o publicly-managed 3 0 .NIA will be t h e l e a d agency f o r t h e two i r r i g a t i o n p r o j e c t s and o t h e r i r r i g a t i o n systems i n which ':he s t u d y s i t e s will b e l o c a t e d . NIA will a l s o be t h e e x e c u t i n g agency f o r a g r i c u l t u r a l development i n ARIP, w h i l e f o r t h e o t h e r s t h e NIA i r r i g a t i o n systms o f f i c e s will be t h e c o o p e r a t i n g a g e n c i e s . MAF will a l s o be a coopi!rating agency i n r e s p e c t of t r i a l s w i t h v e g e t a b l e s . IIMI H e a d q u a r t e r s o r H e a d q u a r t e r s -c o n t r a c t e d S t a f f i n c l u d e a l l i n t e r n a t i o n a l l y -r e c r u i t e d S t a f f as!;igned t o P r o j e c t w o r k .They w i l l i n c l u d e e n g i n e e r s , e c o n o m i s t s , a g r i c u l t u r a : , s c i e n t i s t s , management s t a f f a n d s o c i a l s c i e n t i s t s t o t a l l i n g e i g h t m o n t h s o f t i m e . Research E q u i p m e n t . 3.6.7. There are existing irrigation systems in the Philippines that irrigate diversified crops, particularly in the dry season. Nain system and farm level practices have evolved throush ad hoc procedures undertaken to cope with limited water supply in the dry season. Corresponding agronomic practices for growing non-rice crors have been developed to the extent that production of these crops hase beceme profitable for the farmers.The sources of moisture for ttlese diversified crops are derived from rainfall, diverted river flows for irrigation, and groundwater. Information on crop-water use and production tclchnology for diversified crops is available. However, there is very little information and few guidelines on effective irrigation management for diversified crops in large systems i n the dry season. There i s a clear need to examine the constraints and to identify critical factors for promoting irrigated crop diversification.IIMI is concerned with irrigal.ion management practices that will alleviate the irrigation and assoc:ated factors that inhibit the profitability of irrigated diversified cropping in the service areas of systems with suitable soils during the dry season. One of the objectives of the study is to develop a suitable technology for managing irrigation at the m a i n and distribution systems leve!.~, as well as at the farm level. w a t e r u s e c h a r a c t e r i s t i c s . M o i s t u r e s e n s i t i v e s t a g e s o f c r o p g r o w t h were i d e n t i f l e d . F o r c o r n , optimum w a t e r u s e was shown t o b e e f f e c t i v e f o r g r a i n y i e l d whfin i r r i g a t e d a t t h e t a s s e l i n g and g r a i n f o r m a t i o n s t a g e s i n a r e a s w i t h s h a l l o w w a t e r t a b l e .2 5 . There i s widespread u n f a m i l i a l ~i t y w i t h n o n -r i c e c r o p p r o d u c t i o n under i r r i g a t e d c o n d i t i o n s . At t h e A l l a h V a l l e y , oorn i s grown under r a i n f e d c o n i t i o n s o r through s e e p a g e from a d j a c e n t r i c e f i e l d s . I n d r i e r a r e a s , t h e r e is some a c c e p t a n c e of i r r i g a t e d c r o p d i v e r s i f i c a t i o n , b u t i n a r e a s w i t h s i g n i f i c a n t dry season r a i n f a l l , t h e b e n e f i t s of i r r i g a t e d n r c e p d u c t i o n must be d e m o n s t r a t e d . l'iming of d i v e r s i f i e d crop c u l t i v a t i o n i s i m p o r t a n t because t h e f a c t o r s of temperature, i n c i d e n c e of pests and d i s e a s e s , a n d r i s k of w a t e r l o g g i n g t h r o u g h heavy r a i n f a l l a r e c r i t i c a l . T h e r e s u l t s from C a v i t e show t h a t agroriomic c o n s t r a i n t s can be m i t i g a t e d w i t h a p p r o p r i a t e e x t e n s i o n e f f o r t s . Page 1 production is the foremost consideration of farmers in irrigated agriculture. Where market prices are assured and have comparable stability to rice prices, there is clear evidence that crop diversification can be achieved. In order to alleviate the marketing problem!; for non-rice crops, it is recommended that investigations on the market !itructure and post-harvest facilitiss be undertaken. Other indirect incentives such as reduction o r removal of irrigation fees should be further !studied.36. Better communication between farmers and systems operators should be established. This will reduce the uncertainty over water delivery schedules for irrigating diversified crops need large and intermittent volumes of water. Studies on the joint managsment of systems between the irrigators associations and N I A should be undertaken to fully utilize the capabilities those of organizations in providing effective irrigation service to the farmers.Proposed Phase I 1 Study on Irrigation Nanagement for Crop Diversification 3 1 . Rationale. The results o f tht! initial study showed that there are inportant technical and socio-economic aspects to irrigation management for diversified cropping that should be, addressed. Several constraints were identified, together with suggestetl strategies for promoting irrigated crop diversification. However, these rf:sults must be considered preliminary due to the limited study period ( 2 2 morlths and only one dry season) during which the study was conducted. T o arrive: at more definitive conclusions, further and more detailed study is needed.38. The Study Rdvisory Committee its 13 August 1986 meeting, strongly endorsed the extension or second phase of the study to fully capitalize on the indicative results obtained thus far. Continuation o f the studies on (1) the management o f t t l e main and distributions network of systems, ( 2 ) on-farm irrigation methods and facilities, (3) agronomic practices and ( 4 ) economic and institutional dspects of irrigated crop diversification is envisaged i n thf second-phase study to contribute t o the larger goals o f agricultural productivity in irrigated areas in the Philippines. Proposal focusses primarily on irrigation system management for crop diversification, with a view to asriessing the technical and socio-economic feasibility of several crops grown in limited parts of irrigation systems during the dry season. The Phase 1 1 Proposal will examine (1) the factors that constrain irrigated crop diversification with special attention to the irrigatlon constraints, ( 2 ) ways irl which irrigation practices at the farm and system levels can overcome the5.e constraints ( 3 ) agronomic aspects particularly identifying and testirlg practices for converting puddled t o comparing irrigated rice with rainled nOn-riCe crops, (5) OLN institutionbuilding requirements necessary for. developing and managing irrigated crop upland soil, ( 4 ) the economics o f irrigated non-rice crop production in ","tokenCount":"5828"} \ No newline at end of file diff --git a/data/part_6/0925744000.json b/data/part_6/0925744000.json new file mode 100644 index 0000000000000000000000000000000000000000..b69b4f9112ce4ebbd43409c068f293b3c667478c --- /dev/null +++ b/data/part_6/0925744000.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"88b67ad5990571c60c58e3308ca5913b","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/a969af50-7767-446e-a49e-b8f9274b742c/content","id":"-1216103883"},"keywords":["durum wheat","genome wide association study","GWAS water use","agronomic traits","MTAs","candidate genes","TKW","sedimentation volume","SDS","YR"],"sieverID":"32b199b3-1608-4869-a99f-5f51e597cb51","pagecount":"23","content":"Final grain production and quality in durum wheat are affected by biotic and abiotic stresses. The association mapping (AM) approach is useful for dissecting the genetic control of quantitative traits, with the aim of increasing final wheat production under stress conditions. In this study, we used AM analyses to detect quantitative trait loci (QTL) underlying agronomic and quality traits in a collection of 294 elite durum wheat lines from CIMMYT (International Maize and Wheat Improvement Center), grown under different water regimes over four growing seasons. Thirty-seven significant marker-trait associations (MTAs) were detected for sedimentation volume (SV) and thousand kernel weight (TKW), located on chromosomes 1B and 2A, respectively. The QTL loci found were then confirmed with several AM analyses, which revealed 12 sedimentation index (SDS) MTAs and two additional loci for SV (4A) and yellow rust (1B). A candidate gene analysis of the identified genomic regions detected a cluster of 25 genes encoding blue copper proteins in chromosome 1B, with homoeologs in the two durum wheat subgenomes, and an ubiquinone biosynthesis O-methyltransferase gene. On chromosome 2A, several genes related to photosynthetic processes and metabolic pathways were found in proximity to the markers associated with TKW. These results are of potential use for subsequent application in marker-assisted durum wheat-breeding programs.Wheat is one of the most widely grown crops worldwide (FAO, 2015), and is essential for the human diet [1]. Its importance and worldwide dominance are due, in part, to its agronomic adaptability. Durum wheat (Triticum durum) is a tetraploid wheat species (AABB genomes) mainly grown in the Mediterranean basin, in the Northern Plains (between the USA and Canada), in the arid areas of South Western USA and in Northern Mexico [2]. Durum wheat is well-adapted to a broad range of climatic conditions (including dry environments) and marginal soils, and has low water requirements [3,4]. Climatic conditions, as temperature and water availability, together with biotic stresses, can strongly affect durum wheat development and production [3][4][5][6]. Crop adaptation is a central objective for breeding progress, driving improvement in final production, quantity and quality [7,8]. For over two decades, CIMMYT (International Maize and Wheat Improvement Center) has had an intensive breeding and improvement programme focused on the acceleration of durum productivity in developing countries.Grain quality is an important breeding aim determining product end-use linked to financial returns. It is influenced by both genetic and environmental conditions [9], and biotic and abiotic stresses during growth and at key development stages [10]. Temperature, water availability and soil properties, especially nitrogen content, influence the final quality and protein content of wheat and its end-products [11][12][13].There is a growing need to increase wheat yield without losing grain quality [14,15]. Key end-use grain quality traits include grain protein content (GPC), gluten strength, kernel size and vitreousness [7,16] and are all influenced by climatic conditions [17]. A number of agronomic components influence final productivity, including phenology (maturity) and plant architecture (plant height and lodging resistance). The majority of important agronomic traits, including yield, are controlled or influenced by multiple genes and are quantitatively inherited [18]. In addition, most are influenced by the environment and interactions between environmental and genetic (GxE) effects [19][20][21][22][23]. One of the most common methods currently used for dissection of quantitative agronomic and quality traits is the association mapping (AM) approach [24].AM, originating in human genetics, was initially combined with linkage disequilibrium (LD) to identify the role of genes and linked markers for the determination of disease loci [25]. It is now widely used in plant and crop genetics. Some of the first studies based on LD mapping applied in plants were done in maize [26], rice [27] and oat [28]. AM has the main objective of determining, based on LD, correlations between genotypes and phenotypes in a panel of selected individuals [29]. It can support the development of new genetic markers for use in marker-assisted plant breeding [30]. It also facilitates the analysis of genetic variation underlying traits for further characterisation of the loci of interest [31].Single nucleotide polymorphism (SNP) markers are commonly used in quantitative trait loci (QTL) mapping experiments [32,33] and genome-wide association studies (GWAS) for the detection of marker-traits associations (MTAs) in wheat [34][35][36][37][38]. DArTseq, a variant of the microarray-based DArT technology, has also been widely used in QTL mapping [39][40][41]. It reduces the complexity of the genome, using combinations of restriction enzymes [42] and next-generation sequencing. Several studies have assessed MTAs in durum wheat. The analyzed traits include grain yield, yield and yield components [6,37,43,44], heading date [6], and grain quality traits (thousand kernel weight, vitreousness, protein content, sedimentation index [17,[45][46][47], yellow colour [48,49]).In this study, three panels of elite durum wheat lines from CIMMYT were assessed in field trials conducted over multiple seasons and with differing water regimes. The AM approach was used to detect SNP and DArT markers associated with heterogeneous agronomic and quality trait data in order to test the approach as a tool for marker discovery within a live and ongoing breeding programme.Panels of elite durum lines from CIMMYT wheat preliminary yield trials (PYT), comprising a total of 294 accessions (Supplementary Materials Table S1) were used for agronomic and quality assessment. PYT trials consisted of the best advanced breeding lines which were promoted to unreplicated trials, including one or two repeated checks. The trials were sown, assessed and analysed according to their specific statistical designs [50] and consisted of two blocks with different water treatments, one with full irrigation (FI) and the other with reduced irrigation (RI). In the FI treatment four to five irrigations were applied during the field season to maintain the optimal soil moisture conditions, whilst in the RI block a single irrigation was applied at planting, in order to ensure establishment. In both water treatments the irrigation was applied by gravity in furrows. The rainfall data (https://www.meteoblue. com/en/weather/historyclimate/weatherarchive/ciudad-obreg%c3%b3n_mexico_4013704) for the four field seasons is included in Supplementary Materials Figure S1. The agronomic and quality assessment of the panels over seasons is summarised in Table 1. Wheat panels 2 and 3 were phenotypically assessed across years, while panel 1 was only grown in 2012. The experimental plots (1.6 × 3 m) were sown in November/December of each year and harvested in May of the following year. Data for yellow rust were assessed in semi-controlled conditions at CIMMYT's experimental station in Toluca (Mexico).Plant material for genetic analysis was harvested for each line at the 4th leaf stage (growth stage 14 on the Zadoks scale [51]) and immediately frozen in dry-ice. Samples were stored at −80 • C until DNA extraction. Approximately 100 mg of frozen tissue was used for DNA isolation with a DNeasy Plant Mini Kit from Qiagen, following the manufacturer's protocol. DNA sample quality and concentration were assessed using electrophoresis on a 0.8% agarose gel and the restriction enzyme Tru1I (ThermoFisher) was used to check for the absence of nucleases in DNA prior to genotyping.Samples were genotyped by Diversity Arrays Technology Pty Ltd. (Montana St, University of Camberra, Bruce ACT 2617, Australia) (DArT) using DartSeq TM . A total of 35,509 polymorphic dominant DArT markers and 9142 biallelic SNP markers were generated. Both datasets were thinned by removing one marker from each pair with a correlation coefficient of >0.95. The final dataset consisted on 14,588 DArT markers (of which 8411 were mapped) and 5716 SNP markers (4142 mapped markers). DartSeq TM genotyping and mapping of the corresponding markers to the wheat reference genome sequence RefSeq v1 from the International Wheat Genome Sequencing Consortium (IWGSC, http://www.wheatgenome.org/) was performed by DArT (diversityarrays.com), as described by Sukumaran et al. [43]. The distribution of markers across the A and B subgenomes is given in Table 2. Ten agronomic and quality traits were assessed for three durum wheat elite line panels: days to heading (days, DTH); plant height (cm, PH); lodging (%, LOD); yellow rust (%, YR); yellow colour (YC); sedimentation index (cm 3 , SDS); sedimentation volume (cm 3 , SV); grain protein content (%, GPC); thousand kernel weight (g, TKW); and grain yield (Kg/ha, GY). Agronomic traits (DTH, PH, LOD, YR, TKW and GY) were assessed under both water treatments (FI and RI), and quality traits (YC, SV, SDS and GPC) were only evaluated under FI conditions. Visual disease evaluation and phenology assessments were made in the field, while quality parameters were evaluated on grain samples post-harvest. DTH, PH and LOD and YR were visually assessed at the field trials in Yaqui, while YR was assessed at Toluca. To assess DTH, heading date was recorded as the time when 50% of the spikes have emerged from the flag leaf sheath (stage 59 in Zadoks scale [51]); PH was recorded by measuring the distance between the stem's base and the top of the spike (awns not included); LOD was assessed as the percentage of lodging plot; and YR was assessed as the percentage of leaves with rust pustules. YC was assessed by a rapid colorimetric method with a Minolta color meter following CEN/TS 15,465:2008 [52][53][54]; SDS was evaluated following UNE 34,903:2014 [55,56]; SV and GPC were assessed by Near-infrared spectroscopy (NIRs) [57]; TKW was measured by weighing 2 samples of 100 entire kernels randomly chosen previously dried at 70 • C for 48 h.The correlation between the assessed traits was analysed using the 'cor' function in R [58][59][60]. Then, an analysis of variance (ANOVA) was undertaken, using the 'aov' function in R [61], to obtain the descriptive statistics for each trait.Traits were analysed using a Q + K linear mixed-model [62,63] which follows the model equation:where y is a vector of observed phenotypes; X, S and Z are matrices related to β, α and µ, respectively; β is a vector of fixed effects; α is a vector of marker effects; Qv is a vector of population effect; µ is a vector of polygenic effects (with covariance proportional to a kindship or relationship matrix); and ε is a vector of residuals. These analyses were carried out using GenStat (14th Edition) to generate the best linear unbiased estimates (BLUEs) of variety performance in different ways: (i) across years and blocks; (ii) across years for each block (FI and RI); (iii) across a reduced dataset (years 2013 and 2014) and blocks; and (iv) across the reduced dataset for each block. The resulting datasets (available in Supplementary Material Table S2) were then used in different association mapping analyses.Population structure was assessed using principal component analysis (PCA) based on the combined DArT and SNP genotyping datasets. Euclidean distances were calculated using the R package 'ggfortify' [64] and the PCA was visualised with 'ggplot2' [65].The pattern of linkage disequilibrium (LD) was assessed between each pair of SNP markers on the same chromosome across the two constitutive genomes with the allele frequency correlation (r 2 ) using the 'popgen' package in R [66]. A heatmap was obtained with the D' and r 2 values for each chromosome and a scatterplot to determine LD decay (genetic distance in cM).The AM analyses were performed on the BLUEs obtained above using an additive model with 'rrBLUP' [67] and 'GWASpoly' [68] packages in R in different ways. Two marker-based kinship matrices (k-matrix), created from a subset of 14,588 DArT and 5716 SNP markers, respectively, were used for the adjustment based on relatedness of individuals (Supplementary Materials Tables S3 and S4). A minor allele frequency (maf) threshold of 0.05 was used. To establish a p-value detection threshold for statistical significance of associations, the Bonferroni correction, which employs a threshold of α/m to ensure the genome-wide type error I of 0.05, was applied with a total of 1000 permutations.Associated DartSeq TM and SNP markers were blasted against the wheat reference assembly RefSeqv1 [69] with no indels or mismatches allowed, using an ad hoc Java program, to confirm their physical mapping location on the A or B genomes. The molecular markers were also mapped against the durum wheat genome (https://www.interomics.eu/durum-wheat-genome) to confirm their physical positions. In addition, to identify candidate genes, results were filtered, selecting the hits with best e-value for each molecular marker, and candidate genes were manually selected based on their annotations.Results from the ANOVA for all the traits across years and water treatments are shown in Table 3. The mean phenotypic values across years were calculated for each block and panel to evaluate the influence of water conditions on the assessed traits (Table 3). Days to heading during the field seasons assessed ranged from 63 to 94 days. In plots with lower water availability (RI block), the spike emergence from the flag leaf took place approximately 11 days earlier than in FI plots. Plant height ranged from 39 to 110 cm showing differences between water regimes, with a decrease of 25-30 cm under RI conditions. Likewise, and as result of the RI treatment, GY (ranging from 1.35 to 10.63 ton/ha) and TKW (from 29.6 to 63.2 g) also varied, being reduced by 4-5 tons/ha and 7-10 g, respectively, in the low water availability RI treatment. This strong RI treatment resulted in very low heritability values for DTH, PH and LOD.Several significant phenotypic correlations were observed between the analysed traits (Figure 1 and Supplementary Materials Table S5). The most correlated traits were PH and GY (r = 0. The PCA used a total of 14,588 DArT and 5716 SNP markers. The first and second principal components explained 3.91% of the genetic variation (Figure 2). No underlying genetic structure was detected within or between the panels assessed. LD was estimated using the mapped SNP markers dataset. LD decay was determined within 20-30 cM for all the chromosomes (Figure 3). Using the classification defined by Maccaferri et al. [70], the markers presented loose linkage (Class 2), showing a distance value between 21 to 50 cM.Agronomy 2020, 10, x FOR PEER REVIEW 8 of 27The PCA used a total of 14,588 DArT and 5716 SNP markers. The first and second principal components explained 3.91% of the genetic variation (Figure 2). No underlying genetic structure was detected within or between the panels assessed. LD was estimated using the mapped SNP markers dataset. LD decay was determined within 20-30 cM for all the chromosomes (Figure 3). Using the classification defined by Maccaferri et al. [70], the markers presented loose linkage (Class 2), showing a distance value between 21 to 50 cM. Thirty-seven significant marker-trait associations (MTAs) were detected for TKW and SV across all years and water treatments with most of the significant markers located on chromosome 2A (Table 4). Twenty DArT and seven SNP markers were found in association with TKW on chromosome 2A (with additive effects ranging from −3.41 to 3.46). In addition, eight unmapped DArT and one SNP marker were also associated with TKW (additive effects ranged from −3.39 to 3.46 g). Most of these MTAs showed a negative additive effect, reducing the final weight value (ranging from −2.84 to −3.19 g), and only two MTAs were found to increase TKW (values of 2.97 and 3.09 g). Finally, a single SNP associated with SV was located on chromosome 1B (showing a positive effect increasing the final value by 1.26 mL). The resulting Manhattan and QQ-plots from this AM analysis are included in Supplementary Materials Figures S2-S5.The AM analyses on partitioned subsets of the data consistently detected the QTLs for TKW and SV. Nevertheless, the individual assessment of the water treatments significantly reduced the number of MTAs found, due in part to less available data for the RI block (Supplementary Material Table S6). The initial dataset of 294 durum wheat elite lines was reduced to 200 lines (assessed during the 2013 and 2014 seasons) to give a dataset balanced across assessment years. Using this reduced dataset for AM analysis, the results confirmed the QTLs previously found for the full dataset (Supplementary Materials Table S6). The analysis also detected an additional locus for SV on chromosome 4A, and a locus for YR on chromosome 1B (with additive effects of −0.84 and 2.79, respectively). The marker SNP620, located on chromosome 1B and detected in association with SV, was found included into a cluster of 12 genes encoding blue copper proteins (BCP), with homoeologs in the two durum wheat subgenomes (Figure 4, Table 5 and Supplementary Material Figure S6). In the hexaploid wheat genome, this set of genes form a cluster of homeolog triads [72] with a total of 31 genes (Supplementary Materials Table S7 and Figure S7). Additionally, another interesting gene (TraesCS1B01G568400LC.1) was found closer this marker, coding for the ubiquinone biosynthesis O-methyltransferase.There were several markers located in chromosome 2A, in close proximity to some interesting genes. Markers SNP1183, SNP1184 and DArT3165 were found next to several genes encoding reductase-1 (Figure 4 and Table 5). In addition, the marker SNP8395, also located on the same chromosome, was found in proximity to the gene TraesCS2A01G309700.1, which encodes a type A response regulator 1 (Figure 4 and Table 5).Significant MTAs from the partitioned analysis also allowed the identification of potentially interesting genes. On chromosome 1B, marker DArT1744, previously described by Mérida-García et al. [73] related to high molecular-weight glutenin subunits, was found in proximity to genes encoding isocitrate dehydrogenase kinase/phosphatase G and leucine-rich repeat receptor-like protein kinase family protein. These genes participate in the carbohydrate metabolism during the Krebs cycle and play a crucial role in plant development and stress responses, respectively [74,75]. On this chromosome, another marker (SNP809) was found in proximity to some interesting genes encoding sugar transporter proteins. Additionally, some markers located on chromosome 2A were found in proximity to Acyl-CoA N-acyltransferase genes (SNP1206, SNP8395 and DArT3180) and chloroplastic zeaxanthin epoxidase (SNP1189) (Supplementary Materials Table S8).Agronomy 2020, 10, x FOR PEER REVIEW 13 of 27The marker SNP620, located on chromosome 1B and detected in association with SV, was found included into a cluster of 12 genes encoding blue copper proteins (BCP), with homoeologs in the two durum wheat subgenomes (Figure 4, Table 5 and Supplementary Material Figure S6). In the hexaploid wheat genome, this set of genes form a cluster of homeolog triads [72] with a total of 31 genes (Supplementary Materials Table S7 and Figure S7). Additionally, another interesting gene (TraesCS1B01G568400LC.1) was found closer this marker, coding for the ubiquinone biosynthesis Omethyltransferase.There were several markers located in chromosome 2A, in close proximity to some interesting genes. Markers SNP1183, SNP1184 and DArT3165 were found next to several genes encoding reductase-1 (Figure 4 and Table 5). In addition, the marker SNP8395, also located on the same chromosome, was found in proximity to the gene TraesCS2A01G309700.1, which encodes a type A response regulator 1 (Figure 4 and Table 5). Significant MTAs from the partitioned analysis also allowed the identification of potentially interesting genes. On chromosome 1B, marker DArT1744, previously described by Mérida-García et al. [73] related to high molecular-weight glutenin subunits, was found in proximity to genes encoding isocitrate dehydrogenase kinase/phosphatase G and leucine-rich repeat receptor-like protein kinase family protein. These genes participate in the carbohydrate metabolism during the Krebs cycle and play a crucial role in plant development and stress responses, respectively [74,75]. On this chromosome, another marker (SNP809) was found in proximity to some interesting genes encoding sugar transporter proteins. Additionally, some markers located on chromosome 2A were found in proximity to Acyl-CoA N-acyltransferase genes (SNP1206, SNP8395 and DArT3180) and Table 5. Candidate genes for markers with significant marker-traits associations. Genes located in proximity of markers found in association with TKW and SV across years and water treatments (within a ±50 kb window). Values for physical position and distance are indicated in base pairs (bp). Chr: chromosome position. Blue copper proteins are shown in blue colour; ubiquinone biosynthesis O-methyltransferase in purple colour; the regulator response gene in brown colour; and for reductase 1 genes in green colour. Physical positions and gene annotations are based on the wheat reference assembly RefSeqv1 [69]. The maintenance of crop production is a current and pressing need given growing populations and reduced availability of arable land [76]. There is an increasing need for breeding programs to improve yield potential and the adaptation of new varieties to different biotic and abiotic stresses [77]. Abiotic stresses, including drought and heat, are impacting productivity on the million hectares of wheat grown worldwide each year [78]. Detailed molecular and phenotypic characterization are valuable tools in the dissection of complex traits [79], and especially those that are influenced by water availability [14].The improvement of key traits is essential for better end-use production quantity and quality in wheat [80]. In this study, we analysed a set of 10 agronomic and quality traits under full irrigation conditions (FI), with an additional six traits also assessed under low water availability (RI) in order to understand trait variation under contrasting water regimes in the CIMMYT durum wheat breeding programme. Irrigation conditions influenced some important yield and yield-related traits such as GY and TKW, as well as adaptive traits including DTH and PH (Table 3). The RI treatments had decreased final yields in line with previous observations [6,81]. Previous reports have also shown TKW to be reduced by high temperatures [17], most likely related to water availability. Groos et al. [82] assessed the genetic basis of grain yield and protein content in a segregating population of wheat RILs grown at six locations and also identified effects from GxE interactions involving protein content and yield. Our mean trait values corroborated this, with the highest values for GY recorded for FI blocks across panels (see Table 3). A similar trend was shown for DTH, PH and TKW, which decreased under low-water regimes.Correlations between the assessed traits showed that GY was positively correlated with two different phenology traits (PH and DTH). This is in agreement with Maccaferri et al. [6], who showed important positive and negative correlations for GY and DTH, and also positive correlations for GY and PH in several environments with different water regimes. DTH and PH were also positively correlated (Supplementary Materials Table S5), with taller plants having a longer time period to the emergence of the tip of the spike stage.Wheat TKW is an important yield component with a direct effect on grain yield [83,84]. In line with this, our results showed a significant and positive correlation between TKW and GY. However, the previously reported negative correlation between TKW and DTH [6] was not observed, potentially as result of temperatures and water availability from emergence to heading, and also from heading to harvest. Rharrabti et al. [17] previously highlighted a positive correlation between protein content and TKW, which is in agreement with the results obtained in the present study. They highlighted that warm temperatures during grain filling could negatively affect this correlation.Significant associations between endosperm proteins (gliadin and glutenin subunits) and SV have been previously highlighted [85,86]. Here we found a positive correlation (r = 0.15) between SV and GPC. This correlation is thought to be the result of grain protein content influencing the sedimentation volume value [87], which depends on the degree of protein hydration and oxidation [88]. Finally, for sedimentation index (SDS) analysis, we observed a negative correlation with protein content, in agreement with results presented by Rharrabti et al. [17,45]. This is also in agreement with Oelofse et al. [89] who highlighted the significant influence of protein content on the SDS sedimentation test [90][91][92].The SNP and DArT markers used to analyze patterns of genetic structure (Figure 2) and LD (Figure 3) for the durum wheat lines revealed no detectable genetic structure and consistent patterns of LD across chromosomes (LD was estimated to extend ~20 cM). These results support the suitability of durum elite line sets currently in use in breeding programmes for the practical application of GWAs analysis. The rate of unmapped markers was lower for SNP than for DArT markers (27.5% vs. 42.0%, Table 2), indicating higher precision in genetically mapping SNP markers, probably as a result of co-dominance.In the assessment of MTAs for quality and yield-related traits, different AM analyses were performed on subsets of the dataset. Several MTAs for SV and TKW were detected across years and water regimes, located on chromosomes 1B and 2A, respectively. All GWAS analyses corroborated the major QTLs previously detected, and reported two new QTLs, one for YR in chromosome 1B, and another for SV in chromosome 4A.Associations on chromosome 1B were significant for wheat quality. There are known loci including Gli-B1/Glu-B3 on this chromosome, which are of great importance for some gliadin and glutenin subunits [93]. In fact, the candidate gene analysis reported the presence of a high molecular-weight glutenin subunit (HMW-GS) gene in the proximity of marker DArT1744 (found to be significantly associated with SV and SDS), which was previously described by Mérida-García et al. [73] in association with specific weight. In line with this, Pogna et al. [93] highlighted the importance of Glu-B3 for determining protein quality with these endosperm proteins showing significant effects on SV, which also showed a high and positive correlation with SDS in our study (Figure 1 and Supplementary Materials Table S5). Likewise, Blanco et al. [86] reported three QTLs on chromosomes 1B, 6A and 7B (based on the analysis of 259 polymorphic markers) associated with SDS and SV in a recombinant inbred line population. In the present study, we found a SNP marker (SNP620) associated with SV, showing a positive additive effect of 1.26 (see Table 4) and also with SDS (marker effect of 0.11) (Supplementary Materials Table S6). This marker was previously placed on chromosome 1B, in the same location as MTAs for gluten index and sedimentation index [73]. Other previous studies, such as Reif et al. [94] and Fiedler et al. [95], also reported markers associated with SV on chromosome 1B, but with differing genetic positions. The additional locus for SV found on chromosome 4A (marker DArT9459) has not been previously reported.Marker SNP620, significantly associated with SV, is located within a cluster of homoeolog gene triads coding for blue copper proteins (Table 5 and Supplementary Materials Figure S6). These proteins have been described containing a copper atom, and participate in redox processes [96], with a crucial role in the electron shuttle in plants [97]. In addition, Yao et al. [98] described the blue copper protein genes as the targets of miR408 in wheat, which is involved in the regulation of gene transcription required for heading time [99]. In our study SNP620 was also found in proximity to a gene coding for an ubiquinone biosynthesis O-methyltransferase. Liu et al. [100] highlighted its crucial role as an electron transporter in the electron transport chain of the aerobic respiratory chain. This ubiquinone gene is involved in plant growth and development, is implied in chemical compounds biosynthesis and metabolism which are involved in plant responses to stress, and also participates in gene expression regulation and cell signal transduction [100].On chromosome 1B we also found a significant MTA for yellow rust, in agreement with previous studies in durum and bread wheat, which placed different markers significantly associated with this trait, but in differing genetic positions [101][102][103][104]. The candidate gene analysis revealed the proximity of this marker (SNP809) to sugar transporter protein genes. Sugars are formed during the photosynthetic process and are essential for plant nutrition. The sucrose transport has been considered of great importance for plant productivity [105]. In line with this, the sucrose is involved in the gene expression regulation of the supposedly sugar-sensing pathway [106,107].The majority of MTAs for TKW were located on chromosome 2A, showing both positive and negative effects. Previous studies have reported different markers in association with this quality trait, including a number mapped on chromosome 2A [38,[108][109][110]. One of the markers found by Yao et al. [38] (SSR marker gwm445 on chromosome 2A (68.2 cM), belongs to the same QTL found in this study for the marker SNP1153 (chromosome 2A, 68.6 cM), and also found by Juliana et al. [111] in bread wheat lines from CIMMYT's first year-yield trials. Sukumaran et al. [43] analysed a durum wheat panel of 208 lines under yield potential, heat and drought stress conditions, and identified markers on chromosome 2A with a similar position to those detected in this study (4 markers at 70 cM and 6 markers at 69 cM) under heat stress conditions. They highlighted that several SNP markers were related to transmembranes or were uncharacterized proteins. We found several candidate genes for this important TKW QTL (Table 5) among which the most striking feature is the presence of four reductase 1 genes (NADPH-dependent 6 -deoxychalcone synthase) and a type A response regulator 1 (Figure 4). These genes are both related with photosynthesis. Hu et al. [112] highlighted that NADPH plays a crucial role in biological processes in plants, such as the regulation of the production of reactive oxygen species (ROS) for the stress tolerance [113,114]. Additional GWAS analyses using reduced datasets revealed other interesting genes for this QTL (chromosome 2A, Supplementary Materials Table S8), encoding for the Acyl-CoA N-acyltransferase and the chloroplastic zeaxanthin epoxidase. The first gene has several functions in signaling and metabolic pathways [115]. The zeaxanthin epoxidase plays an important role in the xanthophyll cycle and abscisic acid (ABA) biosynthesis. The xanthophyll cycle has a main function in the dissipation of light energy excess and also increasing the photosynthetic system stability [116].The proposed approach has successfully detected genetic markers with significant associations with TKW, SV, SDS and YR. These are of potential use in durum wheat breeding programs, and can be further interrogated to the candidate gene level using the RefSeqv1 bread wheat genome reference [69] and the durum wheat genome reference [71].The following are available online at http://www.mdpi.com/2073-4395/10/1/144/s1: Figure S1: Rainfall data for Ciudad Obregon (Mexico) for the growing seasons 2012-2015; Figure S2: Manhattan plots for durum wheat mapped DArT markers. DTH: days to heading; PH: plant height; GY: grain yield; TKW: thousand kernel weight; YC: yellow color; SV: sedimentation volume; SDS: sedimentation index; and GPC: grain protein content; Figure S3: Manhattan plots for durum wheat mapped SNP markers. DTH: days to heading; PH: plant height; GY: grain yield; TKW: thousand kernel weight; YC: yellow color; SV: sedimentation volume; SDS: sedimentation index; and GPC: grain protein content; Figure S4: Quantile quantile-plots from genome-wide association studies (GWAS) analysis for durum wheat DArT markers (mapped and unmapped). DTH: days to heading; PH: plant height; LOD: lodging; GY: grain yield; TKW: thousand kernel weight; YC: yellow color; SV: sedimentation volume; SDS: sedimentation index; and GPC: grain protein content; Figure S5: Quantile quantile-plots from GWAS analysis for durum wheat SNP markers (mapped and unmapped). DTH: days to heading; PH: plant height; LOD: lodging; GY: grain yield; TKW: thousand kernel weight; YC: yellow color; SV: sedimentation volume; SDS: sedimentation index; and GPC: grain protein content; Figure S6: Blue copper protein gene cluster on durum wheat chromosome 1B. High confidence genes are shown in green colour, low confidence genes are shown in yellow; Figure S7: Cluster tree of blue copper protein gene homoeologs in bread wheat (RefSeqv1 [69]). For chromosome 1A, high confidence (HC) and low confidence (LC) genes are shown in brown and orange colour, respectively; for chromosome 1B, HC and LC genes are shown in dark and light green colour, respectively; for chromosome 1D, HC and LC genes are shown in dark and light blue colour, respectively; Table S1: Durum wheat elite lines assessed; Table S2: Best Linear Unbiased Estimates (BLUEs) outputs for all assessed traits and the association mapping analyses performed in durum wheat: [i] across years and blocks;[ii] across years for each block (FI and RI); [iii] across years and blocks for a reduced dataset (years 2013 and 2014); and [iv] across the reduced dataset for each block. DTH: days to heading; GPC: grain protein content; GY: grain yield; PH: plant height; SDS: sedimentation index; SV: sedimentation volume; TKW: thousand kernel weight; and YC: yellow colour; YR: yellow rust; LOD: lodging; Table S3: Kinship matrix for durum wheat DArT markers; Table S4: Kinship matrix for durum wheat SNP markers; Table S5: Phenotypic correlations between the assessed traits in durum wheat and their corresponding p values. YR: yellow rust; DTH: days to heading; PH: plant height; LOD: lodging; GY: grain yield; TKW: thousand kernel weight; YC: yellow color; SV: sedimentation volume; SDS: sedimentation index; and GPC: grain protein content; Table S6: Marker-trait associations found for the association mapping analyses performed in durum wheat: [i] across years and blocks; [ii] across years for each block (FI and RI); [iii] across years and blocks for a reduced dataset (years 2013 and 2014); and [iv] across the reduced dataset for each block. SV: sedimentation volume; TKW: thousand kernel weight; SDS: sedimentation index; YR: yellow rust; \"-\": unmapped marker; Table S7: Homoeolog triads for blue copper protein genes mapped in the wheat reference assembly RefSeqv1 [69]; Table S8: Candidate genes for GWAS analyses performed in durum wheat: [i] across years for each block (FI and RI); [ii] across years and blocks for a reduced dataset (years 2013 and 2014); and [iii] across the reduced dataset for each block. Molecular markers mapping positions are shown both in the durum wheat genome [71] and the wheat reference assembly RefSeqv1 [69]; Supplementary Material S1. R script used to perform the GWAS analysis; Supplementary Material S2. R script used to perform the LD analysis. Funding: This research was funded by project P12-AGR-0482 from Junta de Andalucía (Andalusian Regional Government), Spain (Co-funded by FEDER). ARB is supported by the UK Biotechnology and Biological Sciences Research Council (BBSRC) 'Designing Future Wheat' cross-institute strategic programme","tokenCount":"5536"} \ No newline at end of file diff --git a/data/part_6/0990225389.json b/data/part_6/0990225389.json new file mode 100644 index 0000000000000000000000000000000000000000..db65407e88b81abc030a78c4e8bca2e8936b5351 --- /dev/null +++ b/data/part_6/0990225389.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"3bae0275af90830c70db88a1867abd2b","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/0ab982c8-6e87-4ad5-9c9f-35f60ca32b1e/content","id":"-723637332"},"keywords":["Maize","Zea mays","Hybrids","Plant breeding","Seed production","Seed industry","Public sector","Private sector","Germplasm","Development projects","On farm research","East Africa","Southern Africa AGRIS Category Codes: E14 Development Economics and Policies F30 Plant Genetics and Breeding Dewey Decimal Classification: 338.1968"],"sieverID":"3d738949-ec54-483c-967d-1f512a98899e","pagecount":"43","content":"CIMMYT® (www.cimmyt.org) is an internationally funded, nonprofit, scientific research and training organization. Headquartered in Mexico, CIMMYT works with agricultural research institutions worldwide to improve the productivity, profitability, and sustainability of maize and wheat systems for poor farmers in developing countries. It is one of 16 food and environmental organizations known as the Future Harvest Centers. Located around the world, the Future Harvest Centers conduct research in partnership with farmers, scientists, and policymakers to help alleviate poverty and increase food security while protecting natural resources. The centers are supported by the Consultative Group on International Agricultural Research (CGIAR) (www.cgiar.org), whose members includes nearly 60 countries, private foundations, and regional and international organizations. Financial support for CIMMYT's research agenda also comes from many other sources, including foundations, development banks, and public and private agencies.Future Harvest® builds awareness and support for food and environmental research for a world with less poverty, a healthier human family, well-nourished children, and a better environment. It supports research, promotes partnerships, and sponsors projects that bring the results of research to rural communities, farmers, and families in Africa, Asia, and Latin America (www.futureharvest.org).Tables Table 1. Coverage In 1997, the International Maize and Wheat Improvement Center (CIMMYT) launched a major study designed to document the impacts of international maize breeding research in developing countries. The study was intended to update and extend the findings of CIMMYT's first global research impacts study, which had been published three years earlier in a report entitled Impacts of International Maize Breeding Research in the Developing World, 1966-90 (López-Pereira andMorris 1994). Given the enormity of the data collection task, the follow-up study was divided into three regional studies-one each for Latin America, Asia, and sub-Saharan Africa. The sub-Saharan Africa study focused specifically on eastern and southern Africa; a separate impacts study for western and central Africa was conducted by the International Institute for Tropical Agriculture (IITA), which holds the mandate for maize germplasm improvement work in that region. This report presents the results of the eastern and southern Africa study.The results presented in this report are based on information collected during 1998 and 1999 through a comprehensive survey of public and private maize breeding organizations and seed production agencies in 12 countries of eastern and southern Africa. The survey collected information on many aspects of maize research, seed production, and seed distribution (e.g., maize breeding activities, products of breeding programs, maize seed production and sales operations, seed industry regulations and policies). The countries covered by the survey accounted for more than 95% of all maize produced in the region in 1998/99. The organizations contacted as part of the survey currently control about 97% of the total maize seed market in the 12 countries.Major findings of the study are summarized below.In many countries of eastern and southern Africa, policy reforms introduced during the 1980s and 1990s have succeeded in liberalizing national maize seed industries by opening up maize seed markets to increased private sector participation. This represents a major change from earlier years, when maize seed industries in most countries throughout the two regions were dominated by public agencies. Major policy reforms have included the lifting of outright bans on private seed companies, removal of restrictions on importation of commercial maize seed, and elimination of direct seed price controls. In a number of countries, however, implicit restrictions in the form of strict seed certification requirements and lengthy varietal registration procedures continue to limit the participation of private firms, both local companies and multinationals. Moreover, governments in some countries still are trying to influence maize seed prices indirectly by subsidizing maize seed production, especially where public agencies continue to operate and control large shares in the seed market.Throughout eastern and southern Africa, privatesector investment in maize breeding research has been growing rapidly. In eastern Africa, public-sectorDuring the 1990s, the private sector effectively took over the seed supply function throughout most of eastern and southern Africa. In 1996, the most recent year for which complete data are available, private seed companies controlled more than 97% of all commercial maize seed sales in the region. Efforts to privatize maize seed markets were thus largely successful. It is important to note, however, that in a number of major maize producing countries (e.g., Kenya, Zambia, Zimbabwe), the leading private seed company is simply a transformed version of a previously public or parastatal seed agency that had long monopolized the local seed market. In several instances, these privatized parastatals continue to control more than 80% of the national seed market.With privatization, the maize seed industry is becoming more concentrated as a relatively small number of large multinationals acquire, merge with, or buy large shares in local seed companies. While consolidation could lead to scale economies, reduced competition is a concern to farmers, who worry that they may eventually face restricted choices and have to pay higher prices. The fact that seed prices in many countries have not risen significantly may reflect continued attempts by governments to keep maize seed prices affordable by subsidizing public seed production agencies.With the emergence of a flourishing private seed industry, the share of hybrids in varietal releases and seed sales has risen steadily. Coupled with the fact that public breeding programs are also concentrating increasingly on hybrids, this has led to a situation in which varietal releases and seed sales are now completely dominated by hybrids. This could have negative impacts on small-scale subsistence-oriented farmers, many of whom may lack the resources needed to buy fresh seed every season. To date, little empirical evidence has emerged to indicate that small-scale farmers have been adversely affected by the shift to hybrids, but the matter will require careful attention, because supplying seed to smallholders may not be of interest to profit-motivated private firms.Commercial maize seed sales data support estimates made by researchers and seed industry insiders that adoption of improved varieties increased steadily during the 1990s in eastern and southern Africa. However, adoption patterns have differed between countries and regions. Total sales of improved maize seed have fluctuated around 20,000 tons per year in eastern Africa, whereas in southern Africa total seed sales grew steadily in the early 1990s, peaking in 1992 at just under 100,000 tons before declining. Within individual countries, the percentage area planted to improved maize seed varies from less than 10% to nearly 100% in several major maize producing countries. The variability between countries in adoption rates can be attributed to differences in economic, institutional, and policy factors that affect the availability and affordability of improved seed.CIMMYT's maize breeding program has had significant impacts in eastern and southern Africa, especially in recent years. Of all maize varieties released by public and private breeding programs since 1966, about 24% (35% if South Africa is excluded) were developed using CIMMYT source materials. Use of CIMMYT germplasm showed a strong positive growth over time; of the varieties released since 1990, over 31% (55% if South Africa is excluded) were developed using CIMMYT source materials. In 1996, more than 1.6 million ha in eastern and southern Africa were planted to varieties that had been developed using CIMMYT germplasm, representing over 20% of the area planted to modern varieties. Excluding South Africa, where farmers grow mainly temperate materials not targeted by CIMMYT's breeding program, nearly 37% of the area planted to modern varieties in eastern and southern Africa was planted to varieties containing CIMMYT germplasm. Taken together, the varietal release data and the adoption data indicate growing demand for CIMMYT source materials on the part of public and private breeding programs, as well as growing acceptance by farmers of varieties developed using those materials. The observed growth in demand for and use of CIMMYT germplasm in eastern and southern Africa over recent years validates the decision by the CIMMYT Maize Program to establish regional maize breeding programs in eastern and southern Africa and to allocate increased resources to local adaptive breeding.The International Maize and Wheat Improvement Center (CIMMYT) holds a global mandate to increase maize production and boost the productivity of maize-based cropping systems in developing countries. In pursuing this mandate, CIMMYT collaborates with many public, private, and non-governmental organizations (NGOs) involved in technology development and diffusion activities. A crucial component of CIMMYT's strategy is to develop and distribute improved maize germplasm through an international network of maize breeding and seed production organizations.During the early 1990s, CIMMYT carried out a study designed to document the impacts of international maize breeding research in developing countries, the results of which were published in 1994 in a report entitled Impacts of International Maize Breeding Research in the Developing World, 1966-90 (López-Pereira andMorris 1994). The objectives of the study were to document-for CIMMYT, its collaborators, and the donor community-the impacts of international maize breeding efforts in developing countries. The study generated a wealth of useful information about levels of investment in maize breeding research, the spread of modern varieties throughout the developing world, and farm-level impacts of varietal adoption. 1 In subsequent years, CIMMYT's maize research impacts database served as an important source of information for organizations involved in maize research and development activities. Based on the continuing strong demand for this information, the decision was taken to update and expand the database periodically. Accordingly, a follow-up study was launched in 1997. An additional factor motivating the follow-up study was the need to include more and better data from the private sector, given that limited effort was made during the first study to comprehensively survey private seed companies.Given the enormity of the data collection task, the follow-up study was divided into three regional studies-one each for Latin America, Asia, and sub-Saharan Africa. The sub-Saharan Africa study focused specifically on eastern and southern Africa; a separate study focusing on western and central Africa was conducted by the International Institute for Tropical Agriculture (IITA), which holds the mandate for maize improvement work in that region. This report presents the results of the eastern and southern Africa study.The objectives of the eastern and southern Africa regional impacts study resembled those of the Latin American and Asian regional impacts studies:• to estimate the level of public and private sector investment in maize breeding research; • to document the germplasm outputs of public and private maize breeding programs; • to document the use of CIMMYT source materials by public and private maize breeding programs; and • to estimate the rate of farm level adoption of improved maize germplasm.The information and analysis presented in this report are based on data collected in 1998 and 1999 through a comprehensive survey of research organizations and seed production agencies located throughout eastern and southern Africa. A structured questionnaire was used to gather information about each organization's maize research activities, maize seed production activities, and/or maize seed distribution activities. Secondary sources also were tapped for additional information about seed regulations and seed policies.The survey covered 12 countries (Table 1). Collectively, these countries include more than 90% of the total area planted to maize in eastern and southern Africa and account for more than 95% of all maize produced in the region. All major public organizations that engage in maize improvement research and/or seed production were contacted, as were 31 private seed companies (including all of the industry leaders). Between them, these organizations controlled 97% of the total maize seed market in 1996. The survey could not be administered in a few countries due to civil strife, but given that these countries account for a negligible proportion of total regional maize area and production, the omissions are of minimal significance.Data collection proceeded in stages. First, copies of the questionnaire were mailed to directors of national maize research programs and to senior researchers (mainly plant breeders) in public seed agencies and private seed companies. After the respondents had been given time to review the questionnaire and assemble information, they were personally interviewed, in most cases by one or more of the study authors, and in a few cases by experienced non-CIMMYT researchers. Following the interviews, data recorded in the questionnaires were extensively cross-checked for accuracy and consistency. During this process, staff of the CIMMYT Maize Program helped to resolve numerous questions related to the genetic backgrounds of commercial varieties. In a number of cases, the original respondents were contacted a second time to clear up inconsistencies.Maize dominates the food economy of eastern and southern Africa, where it is by far the dominant staple crop grown by the vast majority of rural households.Maize in sub-Saharan Africa is produced in a wide range of production environments. Based on agro-climatic factors and grain maturity characteristics, the CIMMYT Maize Program has identified eight distinct maize production environments, known as mega-environments: Maize production statistics for sub-Saharan Africa show pronounced variability through time, reflecting the vulnerability of Africa's mostly rainfed maize production systems to extremely unpredictable weather patterns (Table 2). Disregarding short-term variability, over the longer term the area planted to maize has been expanding in eastern and southern Africa, growing at an annual average rate of 1.8% from 1961-70 before accelerating to 2.5% during the 1991-97 period. In contrast, no clear long-term pattern has been discernible in western and central Africa; during the past four decades, the area planted to maize in this region alternately expanded and contracted.Maize yield growth has been similarly variable (Table 2). Throughout sub-Saharan Africa, annual growth in maize yields fluctuated around 1% during the 1960s and 1970s before accelerating noticeably during the 1980s and 1990s. In eastern and southern Africa, particularly large yield increases were realized during the mid-and late 1990s as maize production recovered from the devastating drought that affected southern Africa in 1991/92. During the latter part of the 1990s, El Niño-related weather phenomena again disrupted maize production in a number of countries, especially in southern Africa, but on the whole the effects were less devastating than expected.Maize production trends reflect combined effects of area and yield variability (Table 2). In eastern and southern Africa, periods of unfavorable weather have often been followed by periods of favorable weather, which has served to reduce the variability in production growth over the longer term. For eastern and southern Africa as a whole, maize production growth averaged about 2.2% throughout most of the 1960s, 1970s, and 1980s before accelerating sharply during the 1990s. Production growth has been much more variable in western and central Africa and actually fell to negative levels during the 1970s as a result of the Sahelian drought.Consumption of maize is high throughout most of the region, reflecting its role as the primary food staple (Table 3). Maize accounts for over 50% of total calories consumed in eastern Africa and 30% of total calories consumed in southern Africa. In southern Africa, per capita annual consumption of maize averages more than 100 kg in several countries, including Lesotho (149 kg), Malawi (181 kg), South Africa (195 kg), Swaziland (138 kg), Zambia (168 kg), and Zimbabwe (153 kg) (CIMMYT 1999). In eastern Africa, per capita annual consumption is somewhat lower, ranging from a low of 40 kg in Burundi to a high of 105 kg in Kenya. In eastern and southern Africa, as in other developing regions, seed of modern maize varieties reaches farmers through the efforts of many different organizations, including public national research institutes, public and parastatal seed production agencies, private multinational and national seed companies, public international agricultural research centers, NGOs, and farmer cooperatives. Over the years as the institutional and policy environment has evolved, some division of labor has emerged, and many of these organizations have become quite specialized.During the 1980s and 1990s, many countries in eastern and southern Africa made significant progress in liberalizing and restructuring their maize sectors. Policy and institutional reforms targeted both output markets (markets for maize grain) as well as input markets (markets for maize seed). Reform of maize grain markets started earlier, however, and therefore has gone on longer.The distinction between output and input markets is important, because reforms needed to restructure grain markets often differ from those required to induce desired changes in seed markets. In attempting to reform output markets, policy makers are interested mainly in the welfare of consumers, given the importance of maize as the major food staple for the vast majority of the population. In the case of input markets, policy makers are interested mainly in the welfare of producers, since improved maize seed is a key component of maize production technology. The distinction is to some extent arbitrary, however, because output and input markets are linked, directly and indirectly. For instance, efforts to help consumers by imposing a ceiling on grain prices represent a disincentive to maize farmers; if price controls reduce the profitability of maize production, farmers may respond by reducing the area planted, which in turn will depress the demand for seed and reduce availability of maize grain to consumers. Similarly, improved seed is an important input in maize production, the price of which directly influences production costs and hence farmers' income. Thus the availability and price of maize seed influence farmers' production decisions, which in turn affect the total supply of grain maize and determine its price and availability to consumers.Given the importance of seed as a key technology component, the maize seed industry often receives special attention from policy makers (Tripp 1998, Pray andTripp 1998). In eastern and southern Africa, as in many regions of the world, targeted institutional and policy provisions are in place to ensure that national seed industries perform well. For example, varietal registration and seed certification are mandatory in most countries, ostensibly to control the genetic and physical purity of commercial seed sold to farmers. Moreover, policies related to restricting or increasing participation in the seed industry, foreign investment and trade in commercial seed, price controls and intellectual property rights for protection of germplasm ownership are the norm. How have national maize seed industries in eastern and southern Africa been affected by recent changes in the prevailing policy environment? Private seed companies are currently operating in all of the surveyed countries. The presence of an active private seed industry reflects a fundamental shift in policy, as private-sector participation was legally proscribed in most countries until recently. However, implicit restrictions limiting the participation of private seed companies are still in place in most countries. Barriers to entry faced by private firms include lengthy varietal registration procedures and mandatory seed certification requirements (Table 4).One important policy change affecting national maize seed industries in all of the surveyed countries has been the lifting of restrictions on the importation of commercial maize seed. This change promises to be particularly beneficial for small countries in which the limited size of the seed market makes establishment of local seed production capacity uneconomic. It may also benefit countries in which multinational firms have refrained from selling their best hybrids for fear of losing the valuable inbred lines to competitors. In several countries, however, commercial seed imports are still subject to import duties; if these duties are passed along in the form of higher prices, imported seed may be unaffordable for many farmers. While direct controls on maize seed prices have significantly diminished, governments in some countries continue to attempt to influence seed prices indirectly, for example by subsidizing the price of seed produced by public agencies.The recent policy reforms are encouraging, but one area in which significant progress has yet to be made is in the area of intellectual property rights. As of 1998, no country in eastern and southern Africa was a signatory to the International Union for the Protection of New Varieties (UPOV) agreement, although several countries had enacted plant varietal protection laws or had extended patent protection statutes to plant varieties and/or plant genetic materials (Table 4). Yet even if intellectual property regimes relating to plant germplasm are still relatively undeveloped, some organizations are successfully using legal measures to appropriate benefits from investments in breeding research. For example, in Malawi, Mozambique, South Africa, and Tanzania, public research institutes collect royalties from private companies on the use of public varieties. Following widespread efforts to liberalize national seed industries, today there are only four public maize seed organizations operating in eastern and southern Africa, compared to 47 private seed companies (Table 5). Between 1992 and 1998, the number of public maize seed organizations decreased by nearly half, whereas the number of private maize seed companies more than doubled.Changes in the relative numbers of public and private seed companies were reflected in a shift in the composition of seed sales. At the beginning of the 1990s, public and parastatal seed agencies accounted for most of the commercial maize seed sold in eastern and southern Africa, but by the latter half of the decade, private companies were supplying about 95% of the total amount sold (just over 90,000 tons were sold in 1996).In the four countries that still have public seed organizations, only in Tanzania has the importance of the public seed industry declined in the face of growing competition from the private sector. In Angola, Ethiopia, and Uganda, public seed organizations continue to hold sizeable market shares, and private seed companies have made few inroads. The factors that have contributed to the continuing dominance of the public seed industry in these three countries are unique to each country. In Angola, the political atmosphere is not yet ripe for private seed companies to start doing business, mainly due to continuing civil strife. (The eight \"private companies\" listed for Angola in Table 5 are all NGOs, some of which distribute seed at no cost.) In Ethiopia, policy makers have been unusually reluctant to encourage the emergence of a private maize seed industry. In Uganda, commercial incentives needed to attract private-sector investment have been lacking, and the only maize seed company currently in operation is a public seed production project based at Kwanda Agricultural Research Centre.The data presented in Table 5 clearly show that except for the four countries mentioned earlier, during the 1990s the private sector assumed control of national maize seed industries throughout most of eastern and southern Africa. It is important to note however, that in several countries (e.g., Malawi, Zimbabwe, Zambia), the leading private seed company is merely a transformed version of what was previously a public or parastatal agency. Since often these public or parastatal agencies had enjoyed monopoly status, their privatization has left the national maize seed market heavily dominated by a single firm. Although the entry of additional private companies is expected to improve industry competitiveness in the future, currently the transformed parastatals remain in control of more than 80% of the national maize seed market in several countries.The ability of the transformed state and parastatal seed agencies to maintain their dominant position can be explained by a number of factors. At the time of their privatization, these companies inherited well-established networks of seed growers, conditioning and storage facilities, distribution depots, and wholesale and retail distribution networks. Furthermore, these companies enjoyed (and in many cases continue to enjoy) a privileged relationship with the public research systems that have been the source of most of the popular commercial hybrids grown in the region. 2 The situation is hardly static, however, and indeed the structure of many national maize seed industries continues to change. Since the initial wave of privatization, additional concentration has taken 2 For a more detailed review of the evolution of maize seed production and marketing in eastern and southern Africa, see Rusike and Smale (1998), Rusike (1998) Howard and Mungoma (1997), Smale and Heisey (1997), Hassan and Karanja (1997), Rusike and Eicher (1997) and Eicher and Kupfuma (1997).Many countries in eastern and southern Africa feature very strong public maize breeding programs. Historically, these public breeding programs have played a crucial role in transforming local maize production practices. For example in Kenya, South Africa, and Zimbabwe, public maize breeding efforts began more than 50 years ago, and public breeding programs in these countries continue to be the main source of improved germplasm (see van Rensburg 1994, Byerlee and Jewell 1997, Eicher and Kupfuma 1997, Hassan et al. 1998). Until quite place in several countries as a result of mergers and acquisitions among private companies. Although industrial concentration could be beneficial if it allows seed companies to capture scale economies and to pass the resulting cost savings along to farmers in the form of lower seed prices, industrial concentration could also be harmful if it leads to reduced competition in the seed industry. Reduced competition could allow companies to engage in monopolistic pricing practices, which might threaten the affordability of improved maize seed for the small-scale, subsistence-oriented farmer. recently, in almost all of the countries covered by the CIMMYT survey, public breeding programs were the only source of improved maize germplasm adapted to local conditions.Traditionally, public breeding programs in eastern and southern Africa did not engage directly in seed production. Improved varieties released by public breeding programs were usually handed over to public or parastatal seed agencies, which multiplied seed for sale to farmers. The public and parastatal seed agencies often enjoyed a de facto legal monopoly; regulations restricting the participation of private companies in the maize seed industry were common in almost all countries (López-Pereira and Morris 1994).Except for Lesotho and Swaziland, 3 all of the countries covered by the CIMMYT survey currently feature public maize breeding programs that engage in varietal development activities (Table 6). Public and parastatal seed organizations no longer engage in varietal improvement research; the four public seed companies still in operation (in Angola, Ethiopia, Tanzania, and Uganda) have eliminated their research divisions and now serve exclusively as the seed multiplication arm of the national breeding program. Half of the private seed companies surveyed (14 out of 28) maintain inhouse breeding programs and produce improved germplasm. The rest of the private companies that operate in the region either import seed from foreign affiliates or restrict themselves to multiplying seed of varieties developed by another branch of their company located elsewhere in the region.Two international agricultural research centers that are members of the Consultative Group for International Agricultural Research (CGIAR) provide support to national maize research programs in Africa: CIMMYT and the International Institute for Tropical Agriculture (IITA). These two centers, whose mandate is to strengthen and support maize research capacity in developing countries, have developed strong linkages with national maize research programs.Since IITA focuses mainly on western and central Africa, CIMMYT has by far the stronger presence in eastern and southern Africa. Through offices in Ethiopia, Kenya, and Zimbabwe, CIMMYT provides direct and indirect support to national maize programs throughout the two regions. CIMMYT does not provide finished varieties intended for release directly to farmers. Instead, CIMMYT develops and distributes intermediate germplasm products that are designed to be used as inputs into public and private breeding programs.During the 1960s and 1970s, much of the maize germplasm provided by CIMMYT to national breeding programs in eastern and southern Africa originated from CIMMYT's breeding program in Mexico. After it became evident that materials developed in Mexico required substantial additional selection in order to become adapted to African production environments, in 1985 CIMMYT established a major regional research station in Zimbabwe to strengthen its maize breeding efforts in eastern and southern Africa. The function of the regional breeding program in Zimbabwe is to develop stress-tolerant, high-yielding maize germplasm adapted to the mid-altitude environments of eastern and southern Africa. Germplasm products of the CIMMYT regional breeding program include sources of resistance to prevalent biotic and abiotic constraints, open pollinated varieties, and inbred lines for use in hybrid formation.Currently more than 100 seed shipments are distributed each year from the CIMMYT Harare program to breeding programs located throughout eastern and southern Africa. These shipments include more than 200 yield trials (each consisting of a set of elite varieties) and several hundred experimental lines. In accordance with standard CIMMYT policy, germplasm developed by CIMMYT is available free of charge to researchers in public and private breeding programs, both within the region as well as elsewhere throughout the world.Managers of public and private maize breeding programs contacted for the CIMMYT impacts survey were asked to provide information about human and financial investments in maize breeding research. In eastern Africa, the number of maize scientists employed in the public sector far exceeds the number employed in the private sector (Tables 7 and 8). In southern Africa, by contrast, the number of scientists employed in the two sectors is very similar. These numbers suggest that maize breeding in eastern Africa remains largely concentrated within the public sector, unlike in southern Africa, where private breeding programs have made considerable advances.With regards to the intensity of research investment (measured here as the number of research scientists employed per million hectares of maize area planted), significant differences are evident by sector and region. Public research organizations in eastern Africa employ more than twice as many maize scientists per million hectares of maize area planted as public research organizations in southern Africa. This pattern is reversed in the private sector: private breeding programs have concentrated their investments in southern Africa, while largely ignoring eastern Africa.What can be said about the productivity of maize scientists employed in the public and private sectors? Based on the average number of varieties released, scientists employed in the private sector have been twice as productive as scientists employed in the public sector, both in eastern and southern Africa. 4 The figures can be expected to change, however, as the private seed industry matures. Since private seed 4The figures in Tables 7 and 8 may understate the relative productivity of scientists working in the private sector, because the productivity measure for private-sector scientists takes into account only varieties being sold in 1998. By contrast, the productivity measure for public-sector scientists takes into account all varieties released between 1966 and 1998, a much longer period. companies are still relatively new in most countries of eastern and southern Africa, they have been releasing varieties at an unusually high rate in an effort to win a greater share of markets that have long been dominated by public agencies.Investment indicators and productivity indices based on numbers of scientists can be misleading if they conceal significant differences in levels of financial support received by each scientist. Table 9 summarizes data on the cost of supporting senior maize scientists in the public and private sectors (salary and benefits, operating budgets). In both eastern and southern Africa, maize scientists employed in the private sector receive nearly twice as much financial support as maize scientists employed in the public sector. These data may explain the productivity difference between the public and private sectors; private-sector scientists produce twice as many varieties as public-sector scientists, but they do so with double the financial resources.In interpreting the data relating to research investment and research productivity, it is important to remember that the average number of varieties released per scientist is not an ideal measure of research productivity, because all scientists may not have the same objectives. For example, scientists working in public breeding programs typically place greater emphasis on \"upstream\" research activities, such as population improvement, development of special trait materials, and other forms of \"prebreeding\" work, whereas scientists working for private seed companies typically place greater emphasis on development of finished varieties. Furthermore, scientists working in the private sector are usually supported by well-established testing, production, and marketing systems, whose goal is to increase seed sales and maximize profits. By contrast, scientists working in the public sector normally face less pressure to increase sales and profits; instead, they are often encouraged to focus on activities that are expected to generate important social benefits.The impacts of maize breeding research ultimately are felt when modern varieties are adopted and grown in farmers' fields. Farm-level technology adoption decisions are affected by many factors that cannot be controlled directly by breeders, however, so varietal adoption rates provide an imperfect measure of breeding productivity. Widespread adoption of modern varieties indicates that breeding efforts have been productive, but lack of adoption does not necessarily mean that breeding efforts have been unproductive. In many instances breeding programs have developed excellent varieties, only to see adoption stymied by Numbers and types of varietal releases therefore provide useful intermediate indicators for judging the productivity of breeding programs. One objective of the 1998/99 survey was to update CIMMYT's varietal releases database for eastern and southern Africa. Compared to the original 1992 survey, the 1998/99 survey went to much greater lengths to collect data from the private sector. Based on the information provided by many private seed companies, the varietal releases database was updated and considerably expanded.Varietal release data are analyzed in this section of the report. In comparing the results of the 1998/ 99 survey with the results of the earlier 1992 survey, minor differences can be observed in varietal release data. Most of these differences arose because a number of research organizations that were formerly public or parastatal had been privatized since the original impacts report was published; varieties released by these organizations were reclassified as private-sector releases. In addition, two adjustments were made to avoid multiple counting of individual varieties. In cases where two or more organizations were found to be selling the same variety in a given country, the variety was listed only once in the varietal releases database. Similarly, in cases where the same variety was being sold in two or more countries, it was listed only once.Last but not least, it is important to keep in mind that the temporal coverage of the public-and private-sector varietal releases databases is not the same. The public-sector varietal releases database includes information about all public varieties released from 1966 through 1998. Since the data form a complete time series, they provide insights into changes through time in the numbers and types of varieties developed by public breeding programs. In contrast, the more limited private-sector varietal releases database includes information only about private-sector varieties that were being sold in the late 1990s; it does not include information about private-sector varieties that may have been sold in the past but that had been discontinued by the late 1990s. The private-sector varietal releases database thus provides a detailed snapshot of the varieties available in the market in the late 1990s, but it does not provide a complete picture of changes that have occurred through time in the numbers and types of varieties developed by private seed companies.Summary information about the maize varieties released by public breeding programs in eastern and southern Africa between 1966 and 1998 appears in Table 10. Of 133 total releases, one-quarter (35) were varieties developed in eastern Africa, and threequarters (98) were varieties developed in southern Africa. While the absolute number of releases was much greater in southern Africa, since the area planted to maize is also much greater in southern Africa, the number of releases per million hectares of maize was similar across the two regions.Regional differences are evident in the types of materials developed by public breeding programs. In eastern Africa, public-sector releases have been evenly divided between OPVs and hybrids, while in southern Africa hybrids have dominated. Summing across both regions, hybrids have constituted a 30% greater share in the overall number of releases. Interestingly, the composition of public-sector varietal releases has not changed appreciably in recent years. The share of hybrids increased during the 1960s and 1970s before stabilizing in the 1980s at about 55% (Figure 1).Regional differences also are evident in the types of hybrids that have been released. Almost all the hybrids released in eastern Africa have been doublecross hybrids, top-cross hybrids, or varietal hybrids. In contrast, breeding programs in southern Africa seem to have emphasized \"large hybrid vigor,\" as more than 70% of the hybrids released in southern Africa have been single-cross hybrids or three-way-cross hybrids. Single-cross and three-way-cross hybrids are generally very uniform and tend to perform especially well under high levels of management, making them suitable for large-scale commercial farmers. These advantages come at a price, however: single-cross hybrids and three-waycross hybrids are time-consuming and difficult to develop, and because they are characterized by low seed yields, seed is more expensive to produce (Pandey, 1998). Double-cross hybrids, top-cross hybrids, and varietal hybrids are less uniform and generally do not yield as well, but their seed is less costly to produce, and their performance is not as severely affected by seed recycling. For these reasons, they are more suitable for small-scale, subsistence-oriented farmers, who are not always able to provide high levels of management, and who are more likely to recycle seed. These differences in the types of hybrids being released suggest that public breeding programs in each region have targeted different groups of farmers. Public breeding programs in eastern Africa evidently have focused on providing for the needs of the small-scale, subsistence-oriented farmer, whereas in southern Africa the main target has been commercial producers. This difference could be linked to the timing of political independence in the two regions: many countries in eastern Africa achieved independence earlier and therefore were quicker to shift the focus of breeding away from large-scale commercial production systems (which had been favored under colonial rule) to smallholder farming systems (which had traditionally been neglected).Summary information about the maize varieties being sold during the late 1990s by private seed companies in eastern and southern Africa appears in Table 11. Compared to public breeding programs, private seed companies have placed much greater emphasis on hybrids. Out of 137 total private-sector releases, only six were OPVs; the remaining 131 (or 95%) were hybrids. Private-sector varietal releases have originated mainly from southern Africa, reflecting the much greater amount of private-sector activity in that region. Only six varieties have been released by private seed companies in eastern Africa; the remaining 131 have been released by seed companies operating in southern Africa (mainly in Malawi, South Africa, and Zimbabwe). Similar to public-sector varietal releases, the composition of private-sector varietal releases has not changed appreciably in recent years. Summarizing across the two regions, the share of hybrids increased during the 1960s and 1970s before stabilizing in the 1980s at about 95% (Figure 2). In terms of the types of hybrids that have been released by private seed companies, regional differences are evident. In eastern Africa, the number of \"high vigor\" hybrids (single crosses, three-way crosses) has been half as large as the number of \"low vigor\" hybrids (double crosses, top crosses, varietal hybrids). By contrast, in southern Africa \"high vigor\" hybrids have clearly dominated.Taking into account both public-sector and private-sector releases, and adjusting for differences in the area planted to maize in each region, relatively more varieties have been developed for southern Africa (21.6 varieties per million ha maize area) than for eastern Africa (11.0 varieties per million ha maize area). This could be due to the greater diversity of maize production environments found in southern Africa, to the higher level of competition prevailing in the private seed industry in southern Africa, or both.In attempting to discern trends through time in patterns of varietal releases, it is important to remember that the temporal coverage of the publicsector varietal releases database is much more complete than that of the private-sector varietal releases database. Despite the lack of information about private-sector releases in earlier years, however, there has clearly been significant growth through time in the proportion of releases coming from the private sector. Of all new varieties released since 1995, close to 100% have come from private seed companies (Figure 3a). Not surprisingly, given the commercial orientation of private seed companies, nearly 95% of these releases consisted of hybrids (Figure 3b).The ecological adaptation of maize varieties released by public breeding programs and private seed companies in eastern and southern Africa reflects the characteristics of local production environments (Tables 12 and 13). About two-thirds (66%) of all released varieties have been adapted to subtropical environments, which is roughly congruent with the share of subtropical environments in the region. Another 13% of all released varieties have been adapted to lowland tropical environments, and an additional 10% have been adapted to mid-altitude environments. Just under 6% of all released varieties have been adapted to highland environments; these highland varieties The predominant grain color of maize varieties released in eastern and southern Africa has differed by region and by type of breeding program. Among varieties released by public breeding programs, 100% of those released in eastern Africa and 88% of those released in southern Africa have been whitegrained (Table 14). Since white maize is the dominant food staple in both regions, this indicates the strong influence of consumer preferences on public breeding strategies. Among the varieties released by private seed companies, 100% of those released in eastern Africa but only 56% of those released in southern Africa have been white-grained (Table 15). Yellow-grained varieties have been released in South Africa and Zimbabwe, where they are in demand for use as animal feed (mainly in Angola, South Africa, Zambia, and Zimbabwe).Variability by region and by type of breeding program has also been evident in the predominant grain texture of maize varietal releases. In both eastern and southern Africa, public-sector releases have included roughly equal numbers of hard (flint) and soft (dent) grain types (Table 14). However, the predominant grain type has differed between countries. Soft-grained varieties have been relatively uncommon in Malawi (11%) and Tanzania (21%), whereas they have dominated in most other countries (including Zimbabwe, where 100% of all public-sector releases have been softgrained). These differences reflect differences in consumer preferences. In Malawi, for example, most consumers strongly prefer flinty grain types, which has influenced the national breeding program to concentrate on hard-grained materials (Ellis 1959, Heisey and Smale 1995, Rusike and Smale 1998). In Tanzania, farmers similarly prefer flinty grain types, which not only lend themselves more easily to traditional processing methods (hand pounding) but also store better. With regard to grain texture, private seed companies also have demonstrated sensitivity to consumer preferences, although in this case the consumers being targeted are often different. Soft-textured grain types have dominated among private-sector varietal releases, accounting for 100% of all releases in eastern Africa and 84% of all releases in southern Africa (Table 15). Soft-grained dent maize has superior industrial processing qualities that millers prefer and hence is preferred by many commercial farmers.Most of the maize varieties released by public breeding programs since 1966 have been intermediate or late maturing (Table 16). In eastern Africa, 20% of all public-sector releases have been intermediate maturing, and 66% have been late maturing. In southern Africa, 26% of all publicsector releases have been intermediate maturing, and 64% have been late maturing. In contrast, a larger proportion of the varieties released by private seed companies have been early maturing. Of all private-sector releases, 43% of those released in eastern Africa and 18% of those released in southern Africa have been early maturing (Table 17). Breeders in the private sector evidently have placed more emphasis on developing short-duration materials that are less vulnerable to drought occurring late in the growing season.Since many maize-growing environments in eastern and southern Africa are susceptible to drought, especially in areas populated mainly by small-scale, subsistence-oriented farmers, the relatively small proportion of short-duration varieties among all releases suggests that the supply of germplasm suited to these environments is quite limited. In an effort to expand the range of technology choices available to farmers, CIMMYT recently initiated a major breeding project in southern Africa, the Southern Africa Drought and Low Fertility Project. The objective of the project, which is being carried out in collaboration with public NARSs and private seed companies, is to develop materials showing increased drought tolerance and enhanced nitrogen use efficiency. Early results appear extremely promising, and improved germplasm developed through the project is rapidly making its way into breeding programs throughout the region. The CIMMYT Maize Program, working out of its headquarters in Mexico and out of regional offices located in Central and South America, sub-Saharan Africa, and Asia, produces improved germplasm that is distributed to public and private breeding programs around the world for use in developing finished varieties. Since CIMMYT does not release finished varieties, the impacts of CIMMYT's maize breeding efforts are best estimated by investigating the extent to which CIMMYT germplasm has been used by public and private breeding programs. This is not an easy task. Tracking the use of CIMMYT germplasm is difficult for several reasons. To begin with, modern maize breeding is an extremely complex undertaking. The development of OPVs and hybrids normally involves repeated cycles of crossing involving a wide range of source materials; consequently, it is often very difficult to trace the complete genetic history of individual varieties. Documenting the origins of individual varieties is greatly complicated by the fact that the pedigrees (genetic background and crossing history) of most commercial hybrids are confidential, since most private breeding programs and even some public ones are reluctant to disclose pedigree information for fear of providing information that might be useful to potential competitors. Despite these difficulties, for this study an attempt was made to compile information on the use of CIMMYT-derived germplasm by public and private maize breeding programs in eastern and southern Africa. Although it was not possible to obtain complete pedigree information for most varieties, especially commercial hybrids developed by private seed companies, the survey respondents were asked the following three questions about each cultivar that had been developed by their breeding program:1. Does the cultivar contain CIMMYT germplasm? 2. If it does contain CIMMYT germplasm, which CIMMYT population(s), pool(s), and/or inbred line(s) were used in developing the cultivar?3. How were the CIMMYT population(s), pool(s), and/or inbred line(s) used? 5In the analysis that follows, data for southern Africa are often reported in two ways: (a) including South Africa, and (b) excluding South Africa. South Africa is sometimes excluded from the analysis because commercial maize hybrids cultivated in South Africa contain mainly temperate germplasm, most of which comes from the US and Europe. CIMMYT maize breeders do not work with temperate germplasm, so most of South Africa's maize-growing area falls outside the area targeted by the CIMMYT Maize Program. In assessing the impacts of CIMMYT's breeding efforts, it is therefore often appropriate to exclude South Africa.Of all maize varieties released by public and private breeding programs since 1966 and whose parentage is known, 24% were developed using CIMMYT source materials (35% if South Africa is excluded).Use of CIMMYT germplasm has varied by region and by type of breeding program (Table 18). Among public breeding programs, use of CIMMYT germplasm has been more extensive in southern Africa (35% of all public-sector releases for which the CIMMYT germplasm content is known contain CIMMYT germplasm) than in eastern Africa (22% of all releases for which the CIMMYT germplasm content is known contain CIMMYT germplasm). Among private breeding programs, the pattern is reversed; use of CIMMYT germplasm has been more extensive in eastern Africa (67% of all private-sector releases for which the CIMMYT germplasm content is known contain CIMMYT germplasm) than in southern Africa (15% of all releases for which the CIMMYT germplasm content is known contain CIMMYT germplasm; 44% if South Africa is excluded). In interpreting these percentage figures, it is important to remember that the total number of private-sector releases is approximately 10 times higher than the number of public-sector releases.Aggregating across both regions (eastern and southern Africa) and both types of breeding programs (public and private), CIMMYT germplasm shows up much more frequently in OPVs than in hybrids (Table 18).How has the use of CIMMYT germplasm changed through time? Trends in the use of CIMMYT germplasm are shown in Table 19 and Figure 4. In both regions, use of CIMMYT germplasm has increased steadily among public and How has use of CIMMYT germplasm varied across ecological zones? In both regions, public and private breeding programs have used CIMMYT germplasm most frequently in developing varieties targeted for tropical lowland and mid-altitude tropical environments (Table 20). Use of CIMMYT germplasm in developing highland materials has been more variable; none of the highland varieties released in eastern Africa contained CIMMYT material, compared to 40% of the highland varieties released in southern Africa. One interesting finding is that public and private breeding programs have made use of CIMMYT germplasm in developing varieties targeted for temperate production environments; 40% of the temperate varieties developed by Kenyan breeding programs and 14.3% of the temperate varieties developed by South African breeding programs have contained CIMMYT germplasm.Simply knowing whether or not a variety was developed using CIMMYT germplasm provides a useful measure of the impact of CIMMYT's maize breeding program. Even more useful would be quantitative information about the importance of CIMMYT germplasm in the genetic makeup of each cultivar (i.e., the percentage share of CIMMYT germplasm). In the absence of complete pedigree information, unfortunately it was not possible to calculate such a measure.Survey respondents were generally reluctant to provide details about the use of particular CIMMYT population(s), pool(s), and/or inbred line(s), so this information was obtained for relatively few varieties. Within the limited sample for which data were available, Population 21 (Tuxpeño) was the most popular CIMMYT source material, followed by Population 22 (Mezcla Tropical Blanco) and Population 32 (ETO Blanco). All three of these populations are adapted to lowland topical environments.Public and private breeding programs in eastern and southern Africa have tended to use CIMMYT germplasm in different ways (Table 21). Public breeding programs have made extensive use of CIMMYT populations, pools and experimental varieties; most of the time, the CIMMYT source materials have been used directly, with little or no additional selection. In contrast, private seed companies have used mainly CIMMYT inbred lines; in most cases, the CIMMYT lines were subjected to further selection before being used.Aggregating across public and private breeding programs, among all varieties that were developed using CIMMYT germplasm, in 43% of cases CIMMYT source materials were incorporated with little or no additional improvement at the hands of local breeders. Conversely, in 57% of cases CIMMYT source materials were further improved before being used. This suggests that although many national breeding programs have developed the capacity to do their own improvement work, a considerable number of breeding programs-especially public breeding programs-continue to make direct use of CIMMYT germplasm. maize germplasm. Through the questionnaire, individuals with knowledge of the maize sector (e.g., scientists working in public research organizations, representatives of private seed companies) were asked to estimate (a) the total area planted to maize in 1996, and (b) the proportions within that total area planted to local varieties, improved OPVs, and hybrids. In addition, data were collected on commercial seed sales by public seed agencies and private companies in 1996. Based on the seed sales data, and using knowledge of average planting rates in each country, it was possible to estimate the area theoretically planted to commercial seed. These area estimates were then compared with direct area estimates to provide a consistency check.Table 22 shows the estimates made by the survey respondents of the total area planted to maize in each country in 1996, along with the 1996 maize area reported by the Food and Agriculture Organization of the United Nations (FAO). With the exception of Angola 6 and Lesotho, where the FAO figures were about 22% lower than the survey respondents' estimates, in all countries the estimates made by the survey respondents closely matched the FAO data (difference of less than 10%). Aggregating to the regional level, the divergence between two estimates was negligible (1.2% difference in eastern Africa and 2.4% difference in southern Africa).Table 23 shows the estimates of the percentage area in each country planted to improved OPVs and hybrids. According to the national program respondents, in 1996, more than one-half of the maize area in eastern and southern Africa was planted to modern varieties. Adoption rates varied, between individual countries, however. Adoption of modern varieties was highest in South Africa (98%), Zimbabwe (95%), Swaziland (75%), Lesotho (75%), and Kenya (72%). Adoption was lowest in Ethiopia (6%), Mozambique (8%), Tanzania (10%), and Malawi (11%). The pronounced inter-country variability in adoption rates presumably reflects differences between countries in terms of institutional and policy factors, including seed delivery infrastructure, economic incentives to adopt modern varieties, and the strength of the local extension service. These factors may be more important than agroclimatic suitability for the successful diffusion of modern varieties. The varietal adoption estimates reveal interesting patterns in the types of modern varieties being grown in eastern and southern Africa. Generally speaking, use of hybrids was much more extensive than use of improved OPVs. According to the national program respondents, in 1996, hybrids occupied approximately 46% of the maize area planted, compared to only 7% occupied by OPVs. The greater use of hybrids reflects the increasing dominance of the private seed industry.Seed sales data for 1996 collected from public and private companies were used to derive an alternative set of estimates of the area planted to commercial maize seed in eastern and southern Africa. This was done by dividing total commercial seed sales in each country by the average planting rate for that country. In eastern and southern Africa, recommended planting rates for maize generally range from 20-25 kg/ha, although they are lower in some countries, including South Africa (10kg/ha), Lesotho (10 kg/ ha), Uganda (16 kg/ha), and Swaziland (16 kg/ha). Many of the survey respondents indicated that farmers often plant at rates lower than the recommended rate, however, so the recommended planting rates were adjusted downward. Accordingly, an average planting rate of 17.5 kg/ha was used for all countries except South Africa and Lesotho, where an average rate of 12.5 kg/ha was used. These planting rates implicitly accommodate a certain amount of wastage, since the seed sales data were not adjusted to allow for the fact that in most years a certain amount of seed remains unplanted, some farmers replant the same field several times in order to establish a satisfactory stand, some of the area planted to commercial seed is never harvested, etc. Generally speaking, the seed sales-based estimates of the area planted to improved germplasm were consistent with the direct estimates made by the survey respondents (Table 23). In many countries, the estimates based on seed sales data were lower than the direct estimates, but this is expected, since the seed sales-based estimates include only area planted to newly purchased seed, whereas the direct estimates additionally include area planted to recycled seed. Countries in which the direct estimates significantly exceeded the seed-sales based estimates tended to be those in which a large amount of seed recycling is known to occur (e.g., Angola, Tanzania, Uganda). 5a and 5b show recent trends in commercial maize seed sales in eastern and southern Africa. 7 After increasing sharply during the early 1990s, maize seed sales leveled off around 120,000 tons for several years before dropping by more than 25% between 1994 and 1996. The decline in commercial maize seed sales during the mid-1990s can be attributed to several factors. In a number of countries (e.g., Kenya, Malawi, Zimbabwe), economic reforms resulted in the removal of input subsidies and elimination of exchange rate distortions, which had the effect of significantly increasing the price of maize seed. Unfavorable weather conditions also played a role, as a prolonged drought caused the area planted to maize to drop significantly in several major maize producing countries (e.g., South Africa).The commercial seed sales data confirm that the maize seed market in eastern and southern Africa is now almost entirely privatized. By 1996, the most recent year for which data are available, private seed companies controlled more than 90% of the total market (Table 24). The domination of the private sector was pronounced not only in southern Africa, where private seed companies are relatively numerous, but also in eastern Africa, where private seed companies are still relatively scarce. 7 Since it was not possible to obtain complete historical seed sales data for Angola, Angola was excluded from the analysis of trends in seed sales. In terms of market size, southern Africa is a much more important market for private seed companies than eastern Africa; the volume of private-sector seed sales in southern Africa is more than three times larger than the volume of seed sales in eastern Africa (Table 24). For public seed companies, the reverse is true; public seed companies continue to sell more maize seed in eastern Africa than they do in southern Africa (Table 24).Aggregating across the two regions, sales of hybrid seed have grown as a proportion of total seed sales. By 1996, hybrid seed accounted for more than 92% of total seed sales (Table 24 and Figure 6). The only significant exception to the trend toward greater emphasis on hybrids has occurred in southern Africa, where public-sector seed sales have become increasingly dominated by OPVs. This finding can be attributed to the fact that with Angola excluded from the analysis, the only public seed company still operating in southern Africa is in Tanzania, where public seed agencies have made a conscious decision to concentrate on OPVs. Given the current environment, most smallholders in Tanzania cannot afford to purchase hybrid seed and other inputs such as chemical fertilizer.The rise of the private maize seed industry and the related increase in the production of hybrid seed raises difficult questions for policy makers. In particular, it is justifiable to ask whether current trends will lead eventually to further marginalization of small-scale, subsistence-oriented farmers, many of whom are likely to find it difficult to buy fresh hybrid seed every year. To date, no definitive answer has emerged to this important question. Despite the widespread belief that hybrid technology is inappropriate for smallholders, evidence from a number of countries in the region suggests that hybrids can be adopted successfully by small-scale producers. In Kenya (Hassan et al. 1998b), Zimbabwe (Eicher andKupfuma 1997, Eicher 1995), Lesotho, Swaziland and South Africa, smallholders have achieved rates of adoption of hybrid maize that are comparable to those achieved by large commercial farmers.How extensive is the area planted in eastern and southern Africa to maize varieties that were developed using CIMMYT germplasm? In the absence of detailed information about the area planted to individual varieties, the area planted to varieties containing CIMMYT germplasm had to be calculated indirectly based on (1) estimates of the area planted to all modern varieties, (2) quantitative data on the use of CIMMYT germplasm in all varietal releases, and (3) qualitative information provided by public-and private-sector breeders. For most countries, it was assumed that of the total area planted to modern varieties, the proportion planted to varieties containing CIMMYT germplasm was identical to the proportion of all varietal releases that have contained CIMMYT germplasm. Implicit in this approach is the assumption that on average, varieties developed using CIMMYT germplasm have been adopted at the same rate as varieties developed without the use of CIMMYT germplasm. For five countries in which only limited information was available about the CIMMYT germplasm content of varietal releases (Angola, Lesotho, South Africa, Swaziland, Uganda), the proportion of modern varieties containing CIMMYT germplasm was subjectively estimated based on information provided by breeders in the public and private sectors about the use of CIMMYT source materials in their breeding programs.Based on these estimates, it is estimated that in 1996 over 1.6 million hectares in eastern and southern Africa were planted to varieties containing CIMMYT germplasm (Table 25). Of this amount, 0.32 million ha were located in eastern Africa (representing 8% of the total maize area in that region, and 21% of the area planted to modern varieties) and 1.31 million ha were located in southern Africa (representing 12% of the total maize area in that region, and 21% of the area planted to modern varieties). Excluding South Africa, where maize is grown mainly in temperate production environments that are not directly targeted by CIMMYT's breeding program, 16% of the total maize area in southern Africa was planted to CIMMYT derived varieties, representing 47% of the area planted to modern varieties. In 1997, CIMMYT launched a major study designed to document the impacts of international maize breeding research in developing countries. The study was intended to update and extend the findings of CIMMYT's first global research impacts study, which had been published three years earlier in a report entitled Impacts of International Maize Breeding Research in the Developing World, 1966-90 (López-Pereira andMorris 1994). Due to the enormity of the data collection task, the follow-up study was divided into three regional impacts studies. This report has presented the results of the regional impacts study carried out for eastern and southern Africa.The results presented in this report are based on information collected during 1998 and 1999 through a comprehensive survey of public and private maize breeding organizations and seed production agencies in 12 countries of eastern and southern Africa. The survey generated information on many aspects of maize research, seed production, and seed distribution (e.g., maize breeding activities, products of breeding programs, maize seed production and sales operations, seed industry regulations and policies). The countries covered by the survey accounted for more than 95% of all maize produced in the region. The organizations contacted as part of the survey currently control about 97% of the total maize seed market in the 12 countries.Major findings of the study are summarized below.During the past decade, national maize seed industries in eastern and southern Africa have undergone major structural changes. In most countries, policy reforms introduced in an effort to scale back the role of the state have paved the way for increased private sector participation in seed research, seed production, and seed distribution activities. These policy reforms have induced marked changes in the organization and performance of national maize seed industries.The most obvious change has been the emergence of a flourishing private maize seed sector. Private maize seed companies currently operate in every country in the region. This represents a major difference compared to earlier years, when maize research and maize seed production were largely restricted to monopolistic government agencies and parastatals. Major reforms that were instrumental in bringing about this change included the lifting of prohibitions on private seed companies, removal of restrictions on importation of commercial maize seed, and elimination of direct seed price controls.Despite the recent liberalization measures, however, in many countries the participation of private seed companies continues to be constrained by implicit restrictions in the form of cumbersome seed certification requirements and lengthy varietal registration procedures. Moreover, a number of governments are still trying to influence maize seed prices indirectly, for example by subsidizing maize seed production. This practice is especially prevalent in countries in which public agencies continue to operate and control large shares in the seed market.Interestingly, the rise of the private seed industry appears to have happened in the absence of welldefined intellectual property regimes. In most countries of eastern and southern Africa, little progress has been achieved in establishing intellectual property rights and implementing plant varietal protection legislation. Private seed companies apparently are relying mainly on trade secrets approaches to keep their most valuable germplasm out of the hands of potential competitors, thereby protecting the investments they have made in maize breeding research.The recent policy reforms have had a pronounced effect on research investment patterns. Although data on research investment are difficult to come by, private investment in maize breeding research clearly has increased as a share of total research investment in many countries. In eastern Africa, public-sector maize breeders still outnumber privatesector breeders, but in southern Africa the number of breeders is now roughly the same. Numbers of scientists provide an imperfect measure of total research investment, however, since they reveal very little about the cost of supporting each researcher. In eastern and southern Africa, as elsewhere in the developing and developed world, the cost of supporting a senior scientist (salary and benefits plus operating funds) tends to be considerably higher in the private sector. Adjusting for the difference in support costs, total research investment by the private sector probably exceeds total research investment by the public sector, certainly in southern Africa and probably for both regions.The productivity of investments in breeding research can be judged by examining the rate at which breeding programs release new varieties. In both eastern and southern Africa, maize breeders employed by private seed companies have on average released more varieties than maize breeders working in public research organizations. This difference in varietal release rates can be attributed to differences in the quantity and quality of investment, as well as to the commercial orientation of private seed companies compared to public breeding programs, which focus more on noncommercial breeding objectives.The composition of varietal releases reflects steady growth in the role of the private sector. By the late 1990s, all maize varieties released in eastern and southern Africa were originating from private companies. The recent decline in public-sector varietal releases no doubt also reflects reductions in public funding for agricultural research that have occurred in many countries in the region.Seed industry liberalization has led to a significant decline in the role of public seed agencies throughout eastern and southern Africa. Seed sales data make clear that the private sector has effectively taken over the maize seed market in many countries. In 1996, the most recent year for which seed sales data are available, more than 90% of total commercial seed sales were made by private companies. It is important to note, however, that in a number of major maize producing countries (e.g., Kenya, Zambia, Zimbabwe), the leading private seed company is simply a transformed version of a previously public or parastatal seed agency that had long monopolized the local seed market. In several instances, these privatized parastatals continue to control more than 80% of the national seed market. At the time of their creation, these companies already had an edge over potential competitors because they inherited well established networks of seed production facilities, conditioning plants, and distribution depots.With privatization, the maize seed industry is becoming more concentrated as a relatively small number of large multinationals acquire, merge with, or buy large shares in local seed companies. While consolidation could lead to scale economies, reduced competition is a concern to farmers, who worry that they may eventually face restricted choices and have to pay higher prices. The fact that seed prices in many countries have not risen significantly may reflect continued attempts by governments to keep maize seed prices low through the supply of subsidized public maize seed.Since private seed companies have strong commercial incentives to concentrate on hybrids, it is not surprising that the emergence of a flourishing private seed industry has been accompanied by a rising share of hybrids in new varietal releases and seed sales. Because many public breeding programs are now also concentrating on hybrids (because of their superior performance), this has led to a situation in which varietal releases and seed sales are now completely dominated by hybrids. Some observers have raised questions about the increasing popularity of hybrids, pointing out that hybrid technologies may not be suitable for smallscale, subsistence-oriented farmers, many of whom lack the resources needed to buy fresh seed every season. Little empirical evidence has emerged to indicate that small-scale farmers have been adversely affected by the shift to hybrids, but the matter will require careful attention, because supplying seed to smallholders may not be of interest to profit-motivated private firms.Commercial maize seed sales data support estimates made by researchers and seed industry insiders that adoption of improved varieties increased during the 1990s in most countries of eastern and southern Africa. However, significant differences in adoption patterns are evident between countries and regions. Total sales of improved maize seed have fluctuated around a modest 20,000 tons per year in eastern Africa, whereas in southern Africa total maize seed sales grew during the early 1990s to peak at just over 100,000 tons in 1992 before declining. Within individual countries, the area planted to modern varieties varies from less than 10% to nearly 100%. The large differences between countries in adoption rates can be attributed partly to differences in economic, institutional, and policy factors that affect the availability and affordability of improved seed.CIMMYT's maize breeding program has had significant impacts in eastern and southern Africa. Of all maize varieties released by public and private breeding programs since 1966 and whose parentage is known, 24% were developed using CIMMYT source materials (35% if South Africa is excluded). Use of CIMMYT germplasm increased steadily over time; of the varieties released since 1990 and whose parentage is known, over 31% were developed using CIMMYT source materials (55% if South Africa is excluded). In 1996, approximately 1.6 million ha in eastern and southern Africa were planted to varieties that had been developed using CIMMYT germplasm, representing nearly 21% of the area planted to all modern varieties. Excluding South Africa, where farmers grow mainly temperate materials not targeted by CIMMYT's breeding program, nearly 37% of the area planted to modern varieties in eastern and southern Africa was planted to varieties containing CIMMYT germplasm. Taken together, the varietal release data and the adoption data indicate growing demand for CIMMYT source materials on the part of public and private breeding programs, as well as growing acceptance by farmers of varieties developed using those materials. The observed growth in demand for and use of CIMMYT germplasm in eastern and southern Africa over recent years validates the decision by the CIMMYT Maize Program to establish regional maize breeding programs in eastern and southern Africa and to allocate increased resources to local adaptive breeding.","tokenCount":"11567"} \ No newline at end of file diff --git a/data/part_6/1035589605.json b/data/part_6/1035589605.json new file mode 100644 index 0000000000000000000000000000000000000000..1b81b26d4afefe42e739d112deb80cadc4e4cf07 --- /dev/null +++ b/data/part_6/1035589605.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"dba6245ea2b80d25ef808474fd18a364","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/fa664cea-50dc-4d1d-aa3b-815f6fbeee9d/retrieve","id":"-772398633"},"keywords":[],"sieverID":"848aaa9a-431b-4009-a0f5-8a34cb101031","pagecount":"4","content":"Tackling gender inequalities is becoming increasingly important for voluntary sustainability systems to address. More and more, sustainability systems are looking to integrate gender into their standards and the management of their organisations. Sustainability systems that are not gender-responsive can result in unnecessary health and safety risks for women and girls, and lead to unequal impacts and unintended consequences. This briefing note provides a collection of good practices to support sustainability systems, including ISEAL Community Members, to get started and progress on gender issues.A CGIAR-ISEAL scoping study on the state of gender integration among ISEAL Community Members in the agri-food sector highlighted three steps to approaching gender equality in sustainability systems: minimum prevention of harm within standards, proactively supporting gender equality within standards, and promoting gender equality and women's rights beyond the standard. This briefing primarily focuses on the first and second steps that address gender equality within standards and draws examples of good practice from ISEAL Community Members.At the bare minimum, standards should have basic requirements to protect women from harm. Key areas include:• anti-discrimination and equality of treatment • zero tolerance for harassment or violence • accountability requirements • gender-specific fair working conditions Below are some examples of how these have been incorporated into standards. • policy/strategy requirements for members and producer organisations • representation (from ground-level committees through to organisational governance and internal processes)• enabling environments and systems changeA significant step that standards can take to proactively support gender equality is to require their members to have their own gender policies and strategies in place.For Fairtrade International requires smallscale producer organisations to develop and implement a gender policy, ensure members are aware of this policy and its contents, and involve women in the development and implementation of the policy.Examples of topics that can be included in a gender policy, from the Fairtrade Standard for Small-scale Producer Organizations 4.3.1 are:• promoting the participation of women in boards, leadership positions and other structures within the organisation • measures against sexual harassment • a grievance mechanism • collection and use of gender-disaggregated data • investing in projects and programmes focusing on women's needs.Standards can support gender equality by requiring women's improved representation at various levels. At the ground level, this can include ensuring that committees include representation of diverse groups, that grievance committees are gender-sensitive and that there is a specific gender committee. At an organisational level, gender equality can be promoted through employment practices. ","tokenCount":"404"} \ No newline at end of file diff --git a/data/part_6/1035627754.json b/data/part_6/1035627754.json new file mode 100644 index 0000000000000000000000000000000000000000..05446bca2543c4656947002c9d83c208e83ab621 --- /dev/null +++ b/data/part_6/1035627754.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"c43bd562cf4794036c0a22a457b8dd7b","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/dda61fd7-f6ee-4447-b9df-81c4464f95fe/retrieve","id":"-1088660241"},"keywords":[],"sieverID":"cd66f055-95f1-40a2-a678-d896b1e9d2f9","pagecount":"8","content":"Comparative genomics is the analysis of genomic relationships among different species and serves as a significant base for evolutionary and functional genomic studies. GreenPhylDB (https://www. greenphyl.org) is a database designed to facilitate the exploration of gene families and homologous relationships among plant genomes, including staple crops critically important for global food security. GreenPhylDB is available since 2007, after the release of the Arabidopsis thaliana and Oryza sativa genomes and has undergone multiple releases. With the number of plant genomes currently available, it becomes challenging to select a single reference for comparative genomics studies but there is still a lack of databases taking advantage several genomes by species for orthology detection. GreenPhylDBv5 introduces the concept of comparative pangenomics by harnessing multiple genome sequences by species. We created 19 pangenes and processed them with other species still relying on one genome. In total, 46 plant species were considered to build gene families and predict their homologous relationships through phylogenetic-based analyses. In addition, since the previous publication, we rejuvenated the website and included a new set of original tools including protein-domain combination, tree topologies searches and a section for users to store their own results in order to support community curation efforts.Plant comparative genomics resources usually compare reference genomes to compute homology sequences and en-able functional annotation transfer (1,2,3,4,5). However, with the growing number of whole genome sequences available within the same species, it has been shown than a single reference is not enough to capture its total genetic diversity (6). A pangenome, usually defined as the full gene repertoire within a species, can be partitioned into core genes that are shared by all individuals and dispensable genes that are present only in a subset of individuals (6,7,8). Characterizing them can have a great potential in plants for crop improvement (7,9,10) as candidate genes can potentially be missing in the genotype used to set up a reference genome. Pangenomic studies have recently been conducted in several crops, revealing significant differences with presence absence variations (PAVs) and/or copy number variations across genotypes (11,12,13,14,15,16,17,18). It became obvious that distinguishing core from dispensable genes is important as dispensable genes can be associated with useful trait diversity (10). Finally, PAVs can have an influence on orthology detection as specific genotype gene losses can lead to false negative results in interspecific comparisons or to pseudo-orthology (19).Until now, comparative genomics databases have not fully taken advantage of these new datasets. Here, we present an updated version of the GreenPhylDB, a database that features multiple genomes for 19 species (e.g. rice, maize, banana, grape and cacao) as well as 27 other species with single reference genomes, for a total of 46 genomes. Publicly available genomes were processed to generate representative pangenes (i.e. a set of representative or consensus sequences) for species that were used in multi-species sequence clustering. Resulting gene families were functionally annotated and analysed with orthology detection methods.We retrieved 132 publicly available datasets (coding DNA sequence and protein-coding genes) for 46 (Supplementary Table S1) and assessed their gene annotation predictions using BUSCO Plants v3.0.2 (embryophita odb10) (20). We checked that the number of CDS was consistent with the number of proteins and that they shared the same locus tag name. When protein-coding genes were missing, we generated the sequences from GFF files originating from data providers. Finally, alternate splices were filtered, and the longest sequence was conserved.Out of the full dataset, 105 genomes were considered to produce 19 pangenes computed with the get homologues-est software v20092018 (21) (Table 1), based on NCBI Blast-v2.2 and using the following program options (-M -F -t 0 -m cluster) as previously applied to the Brachypodium distachyon dataset (13). We processed each cluster the following way : i. For single-gene copy clusters (a single sequence per genome), protein sequences were aligned using MAFFT v7.313 (22) (parameters adjusted according to the number of sequences) and an automatic procedure to generate a consensus sequence was applied (Figure 1A). For each position of the alignment, we kept the most frequent amino acid. In case of a tie, the amino acid of the genome with the highest BUSCO scores ('complete' then 'fragmented' and finally lowest 'missing' scores) was selected. Finally, if more gaps than amino acids were present, this position was removed from the sequence. ii. For multi-copy clusters (multiple sequences per genome), we applied the same procedure as for singlegene copy clusters but added a preliminary step to select a representative sequence by cluster. Multiple sequence alignments were used to generate a distance matrix using distmat (Jukes-Cantor correction method) from EMBOSS v6.6 (Figure 1B). The matrix was required to define the distance for each sequence of all other genomes and the sequences with the smallest sum of distance was selected as representative of the considered genome (Figure 1C). Then, the consensus step was applied. It is worth mentioning that get homologs-est generated sequence clusters of not too distantly related sequences. Large gene families can include several multi-copy clusters being grouped together at the sequence clustering step. iii. For genotype-specific clusters (paralogs in a single genome), we generated a distance matrix between all sequences and the sequence with the lowest average distance (min(d/sum(d))) of all sequences was putatively considered as the most representative sequence. Those sequences were added to the pangene. iv. Finally, singletons (cluster of one sequence) were searched for similarity using DIAMOND (23) with a default e-value on the protein-coding genes of all other species genomes to predict their putative prediction accuracy. Sequences with a minimum of one hit in at least two species were added to the pangene; otherwise sequences were excluded.As a unique identifier was required for each pangene, we defined a nomenclature with a prefix composed of the [5-letter UniProt taxonomy database code] pan, followed by p (for protein) and an auto-increment of 6-digits (e.g. musac pan p029014 for Musa acuminata).Pangenes and protein-coding genes of reference genomes (without pangenes) were searched all against all using DI-AMOND. We then performed a clustering using Tribe-MCL (24) (M = 1.2, 2, 3 and 5), defining 4 levels of stringency (from 1 to 4) to take into account potential subclassification and we obtained 9419, 18 805, 23 409 and 29 345 clusters, respectively.We then scanned all sequences for protein domain signatures using InterProscan (25,26) and also crossed linked matches with UniProtKB-SwissProt entries (27). Cluster names resulting from curation from previous GreenPhylDB versions (2,28) were transferred when at least 51% of sequences were found clustered together as before (based on species in common between releases). In addition, for this release, we implemented an automatic method to name clusters based on the name of InterPro domains (family type only) that were found specific to clusters. In other words, when detected in at least 51% of the sequences composing an unannotated cluster, the name of the InterPro signature was assigned to it. In total, GreenPhylDB comprises 3538 clusters functionally characterized across the four levels.The previous phylogenetic-based methodology that we applied in the previous version has been conserved but uses a larger set of genomes to update our automated pipeline. The pipeline uses MAFFT for the multiple alignment step. FastTree 2 (v2.1.11) (29) was preferred over PhyML (30) due to the size of the clusters. Gene rooting and orthologous scoring was computed with Rap-Green (31) using the viridiplantae species tree extracted from NCBI taxonomy and converted into PhyloXML (2). We successfully produced gene trees at level 1 for more than 99.8% of the clusters (n = 9413) which enabled us to predict ∼17.8 million of orthologs and ∼1.8 million of in-paralogs (or ultraparalogs) relationships. The pipeline was complemented by a Reciprocal Best Hits (RBH) method--computed between all pairs of genomes--that resulted in more than ∼12.1 million orthologous relationships.With this updated version, the website has received a facelift. It now takes advantage of the bootstrap and D3.js frameworks to improve the user experience and to be more responsive. Alternatively, it can also be accessed programmatically using Resource Description Framework (RDF) as implemented in AgroLD, a knowledge-based system relying on semantic web technologies (32).All cluster (or gene family) pages present the same type of information divided into several tabs :1. Gene family composition: a bar chart allows users to visualize at a glance the composition of the gene family by species (Figure 2A). Species are ordered taxonomically to easily detect possible variations between phyla. Each bar is clickable and produces a ta-ble with the list of sequences and associated crossreferences (i.e. InterPro, UniProt). Sequences can be exported in multiple formats and/or stored in a user list. 2. Gene family structure: sequences are clustered at four levels of clustering, from less stringent to more stringent, in most cases narrowing the number of sequences (Figure 2B). 3. Protein domains: here, InterProscan was used to assess the domain conservation consistency and the specificity of the sequence clusters (Figure 2C). For each cluster, we performed statistical analyses to determine whether InterPro signatures were specific and therefore not found in any other sequences of clusters of the same level. 4. Phylogenomic analyses: this section includes multiple sequence alignments that can be downloaded and visualised using MSAviewer (33) and gene trees can be explored with InTreeGreat (https://www.southgreen.fr/ content/intreegreat-tool) and PhyD3 (34) (Figure 2D). Some gene trees can be very large, and the interface proposes an option to prune automatically the tree based on user choice for a range of species. 5. Homologous predictions: the interface enables users to display and refine all the homologies detected by the phylogenetic-based approach and Reciprocal Best Hits (RBH). It is possible to filter and select only a subset of species of interest.The new page type for pangene sequences is a central and unique concept in this version as they were used for the clustering and homology predictions instead of all individual sequences that compose it. When browsing these pages, users can quickly see which genes are present or missing by looking at the status: core or dispensable compartments.Then, information related to the sequence composition is reported. Users can access information about pangene classification, the consensus sequence (except for singletons) with the multiple sequence alignments used to create it as well as related homology predictions (Figure 3). In the case of multi-copy clusters, it is possible to see which sequence was selected as representative (.rep) or participant (.p) and also why they were selected by browsing the distance matrix.The database can still be searched via keyword searches or by entering a query sequence for similarity search using DI-AMOND (which replaces BLAST for faster processing), enhanced by new tools to further explore those datasets.Quick search. A new interface has been designed to retrieve in a comprehensive and concise way all the information associated with gene family names, sequence annotation and annotations from InterPro and UniProt mappings. ularly helpful when searching for transcription factors for which sequences must contain some domains but not others as the Markov Cluster Algorithm (MCL) may fail grouping them accurately. The interface allows the use of various operators (e.g. AND, OR, NOT, ONLY) to filter a set of sequences for all genomes. Results can be compared with the MCL automatic clustering to check consistencies or differences.TreePattern --Tree topology search. A tree search can be done by filtering on gene tree topologies (31). Users can draw the topology with species or taxonomic groups as leaves or nodes of the tree and apply constraints such presence or absence of duplications. Resulting trees can be accessed individually (or exported in bulk results as CSV file) and defined patterns are highlighted. This feature is useful for identifying gene families with an expected evolutionary scenario due to gene duplications.While automatic clustering is a relevant and efficient starting point, sometimes limitations (e.g. missing sequences, er-rors in gene annotations) are present and prevent access to ready-to-use datasets, justifying a deeper characterization that will eventually lead to a refinement of the automatic clustering. As a result, knowledge generated on individual gene families is often available only in publications and their supplementary information as PDFs. To encourage knowledge capture, we developed a section for advanced users to create and share their own gene families. Two methods are possible: users can either start from scratch and upload their data or use existing clusters and take advantage of multiples operations implemented in the 'MyList' features: such feature was indeed developed to facilitate intersecting, combining clusters. This new tool can be valuable during the review process by providing a unique identifier to referees--and eventually to users--to explore the structure and composition of the submitted gene family.In this section, we describe three possible uses that are enabled by this new GreenPhylDB version. Concrete examples related to each of them are further documented in Supplementary Data. This new version of GreenPhylDB provides a unique way to scale up plant comparative genomics studies across multiple plants species by leveraging pangenomic datasets. This release paves the way to the transition from reference-based genomics to pangenome-based systems and tools. In this context, the website includes new powerful search interfaces to explore the content of the gene family collection. Advanced users can also deposit the results of their expert gene family curation for further use and reference. GreenPhylDB is an important resource to understand the genetic basis of genome diversity among plant species and has the potential to accelerate gene discovery to support crop improvement.","tokenCount":"2207"} \ No newline at end of file diff --git a/data/part_6/1090138271.json b/data/part_6/1090138271.json new file mode 100644 index 0000000000000000000000000000000000000000..19cad30a0a760ab280baa4b48e2d3dc8506d9ff1 --- /dev/null +++ b/data/part_6/1090138271.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"b73445c34e3c06e7acd000882dd1338d","source":"gardian_index","url":"https://repository.cimmyt.org/server/api/core/bitstreams/b0de483f-34d1-4bbd-af2d-99bcd3c005c0/content","id":"946609345"},"keywords":[],"sieverID":"72b07aed-ec03-47ae-be4f-a495d45ab15e","pagecount":"6","content":"This report describes the process carried out for the development of a component in the e-Agrology mobile application, which allows the farmer to accept informed consent, thus authorizing the sharing of data on their agricultural production.Development of a component for the acceptance of informed consent to share production data.As part of the activities of the digital initiative, the need to generate a methodology to obtain the consent of the farmer so that he/she authorizes the sharing of his/her production data with third parties was raised, to start the automation of the consent the initial approach was to develop a module within the mobile application of the e-Agrology system, for which we worked together with Digital Green, a global development organization whose objective is to empower small farmers to get out of poverty by harnessing the collective power of technology.The need for this development arose after more than 3 years of collecting agronomic data from projects in Mexico where it was necessary to collect the consent of the farmer, in each of the cycles the collection of consent has been done in a traditional way through the signature of the consent on a sheet of paper, then the sheet was scanned and sent by email with all the compiled forms. Subsequently, a manual cross-referencing of the consents with the e-Agrology system database was performed.The proposal generated by Digital Green consisted on the following: Yellow Component:1. Consent is obtained through the e-Agrology mobile application, this would be done by adding a module in which the farmer views a video and through a question authorizes or declines to share the data, these records would feed the main database of e-Agrology. 2. Cleaning of the agronomic database through an ETL process, generating at the end a database with the transformed data.Green Component:1. Copy of the clean database which in turn feeds a third database that combines the agronomic data with the authorizations to be shared. 2. FS Connector: Development of Digital Green that allows the extraction of data to certain users who have been previously authorized to do so, the user logs into the application and it performs a filter to the information allowing the extraction of an excel which is sent to the end user's email.During the first half of 2023, work was done in together with Digital Green on the implementation of this solution and some testing of the Farmstack Connector was conducted.Image 2. Farmstack connector login page in operation.where the consent clause is read. The acceptance of the farmer's consent is done by saving his fingerprint, in this step it is necessary that the technician performs a validation by means of his previously saved fingerprint.In case the registration is done online the approved consent is sent to the server at that moment, otherwise it is stored only on the phone until it is connected to the network.Once the data is saved, the database is cleaned by means of an ETL tool, and a database with clean information is generated. Finally, with the Farmstack Connector component developed by Digital Green, it is possible to perform queries to this database and process the information outputs according to the user's authorization.Currently completed in the mobile version of e-Agrology is the development of the consent component that allows to save the fingerprints of the farmer and the technician, the next step to complete the development is the connection of the Farmstack Connector of Digital Green with the clean database.","tokenCount":"574"} \ No newline at end of file diff --git a/data/part_6/1116537258.json b/data/part_6/1116537258.json new file mode 100644 index 0000000000000000000000000000000000000000..e630ea8c93a03a14828d96e7ad63b888e9684180 --- /dev/null +++ b/data/part_6/1116537258.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"a4f36a1163990b7f73abd8082c3f5a20","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/abd08311-1a17-4073-88af-268cf7843b96/retrieve","id":"-1520990606"},"keywords":[],"sieverID":"c91c1d25-c128-4709-80ac-d7489a5c0b25","pagecount":"14","content":"Highly pathogenic avian influenza (HPAI) is an infectious disease in poultry and can result in high mortality in domestic poultry. Infections in poultry occur as a result of direct contact with infected birds. These could be prevented by good husbandry, nutrition and vaccination. The government has put in place measures to improve poultry farming such as training farmers on biosecurity measures, improving quarantines and live bird markets, vaccination and provision of compensation to affected farmers.The study was carried out Nigeria with an aim of assessing the effectiveness of biosecurity training among the poultry farmers. A total of eight states were included in the study. These were later grouped into four clusters where each cluster comprised of a high risk state paired with a low risk state so that we had: With the aid of secondary data farmers that were trained on biosecurity practices were identified. The households were further classified into four categories:From the four categories, three farmers were singled out in each state to hive an overall sample size of 96 farms. A total of 82 poultry farmers participated in the survey. The data was originally in access then imported into STATA 11.1 for analysis. The data contained three categories of variables; those that described the farm and respondent characteristics, the independent variables and the outcome variables (biosecurity related variables).All the variable names were not clear hence renamed.Those that were missing labels were labelled for comprehensibility. Variables like city, town and village had to be dropped because they had very few records thus did not give sufficient information. Location was then used in place of these variables as it had more records and also covered a lot of information about the above stated dropped variables.Individual responses especially string entries in the open ended questions were verified with invalid ones being dropped and others summarised to make sense. In cases where the respondent was allowed to give more responses outside the list of those provided, such entries too were verified where most of them that had close meaning to those in the choices were fitted into the most likely option.The data was also checked to identify irrelevant entries for instance where a farmer gave a negative answer to a question then further gives a positive answer to a question related to that which he had earlier on responded to negatively, entries of this sort were dropped.Ambiguous entries were deleted these included cases where counts for animals or workers were given as decimal numbers take for instance the number of poultry attendants on a farm.Other procedures involved in cleaning the data included combining multiple response variables and generating new ones for example the variable on farmers' sources of veterinary services was recorded as a set of indicator variables, one for each possible response, these variables were combined and a new one generated so that we had 4 sets of variables representing the sources of veterinary services The data cleaning process further involved procedures such as encoding string variables to numeric as STATA does not recognize string variables.Scores used ranged from 0 (worst biosecurity practice) to 4 (best biosecurity practice). A new variable containing the sum of the scores of the biosecurity related variables was generated.Principle component analysis was used to get a small set of variables which were uncorrelated with each other hence avoid the problem of multicolinearity. Normally, when using principle component analysis, cases that have a missing record are automatically deleted, to avoid such loss of data, all the missing entries in the biosecurity related variables were recoded to zero after which principle components were derived. Normally, the first principle component explains the most variation in the data so using the factor scores from the first principal component analysis as weights; a dependent variable was constructed for each farm. The indices derived were relative measures of the implementation of the biosecurity measures, the higher the index the higher the implementation of the biosecurity measures. This variable was used as the dependent variable in the regression model. To test whether training had an influence on the biosecurity practices mixed models were used having biosecurity index, training, gender age, distance of closest poultry farms and number of chicken kept as the fixed effects and state as the random effect.To validate the model, residuals were first obtained and the check for normality performed on the model's residuals using the command knorm. The results obtained showed indications of normality.The pnorm command was also used and the result obtained also indicated that the model's residuals had a normal distribution. Majority of the respondents (62% n=49) were males. Of the farmers interviewed the most dominant were those aged 31 years and above and only 5(6%) below 30 years. The number of birds kept were categorised into 4 groups (Table 3). Only 20(24.39%) farmers kept less than 20 chickens while majority kept more than 320, indicating that large scale poultry farming is a common practice among the farmers. Most farmers (82% n=63) cited eggs as their main chicken production type. Of those interviewed Fifty seven per cent (n=45) of the respondents' poultry had not been infected with HPAI in the past while thirty eight per cent (n=30) had been infected.it was also noted that a further 87 %( n=68) of the respondents had never vaccinated their poultry against Highly Pathogenic Avian Influenza. On the contrary, a greater number (94% n=75) said they had used vaccines to control Fowl cholera and Gumboro diseases.Majority of the farmers (68% n=48) had registered their farms, they went on and suggested that they had registered their farms as the government required them to do so. It was also noted that most of these farm registrations took place in years 2006 and 2007. When further asked if the indicated biosecurity measures they had implemented on the farms were still in place, majority of the farmers had the above measures still in place. Only 8 out 51 (15%) farmers had not been able to train someone else using the knowledge they had obtained from these courses.Thirty eight per cent (n=31) of the farmers interviewed cited they had had a HPAI outbreak or suspected HPAI infections on their farms in 2006(n=14 48%) and 2007(n=15 52%).A higher proportion of trained farmers (n=17 74%) reported the outbreaks/suspicions to the authorities. Eighty eight per cent (n=30) of all the farmers that had outbreaks on their farms said that the HPAI team made a follow-up on the outbreak/suspicion after reporting. Both trained and untrained farmers had the carcasses sampled. Of the infected farms, thirty two per cent (n=9) indicated that the carcasses were sampled by NAICP desk officers, response team (32% n=9), state/private veterinarians (18% n=5), NVRI (14% N=4) and other (4% n=1). The types of samples collected as indicated by the farmers in decreasing order include:  Carcasses(54% n=14)  Blood and carcasses(19% n=5)  Carcasses, swabs and blood(8% n=2)  Carcasses, blood and serum(4% n=1)Out of 35 farms,28(80%) were disinfected by the field team during the first outbreak, PPE equipment was used in 30 farms(86%) and culls were conducted in the surrounding area in 21 farms(60%) *There was a significant association between training and the mode of selling eggs, a larger proportion of trained farmers (24% n=10) sold and delivered their eggs to traders while none of the untrained farmers practised that.There was evidence that training (p=0.038) was significant. Farmers who had been trained on the biosecurity measures were better than the untrained ones. Those aged between 41 and 50 years were noted to be better enactors of the practices. However the gender of the farmers was not significant. Both female and male farmers had similar practices. Proximity of the other farms did not influence the implementation of the biosecurity measures taught. The variables used to generate the indices had quite a number of missing entries.  Poor variable names which were difficult to comprehend hence had to rename all the variables.  Poor coding -some variables were not properly coded where codes were assigned to non-existent groups and even missing values were coded. As a result some codes had to be replaced for accuracy.","tokenCount":"1349"} \ No newline at end of file diff --git a/data/part_6/1117663117.json b/data/part_6/1117663117.json new file mode 100644 index 0000000000000000000000000000000000000000..7bf42cf28e4d9d73b0abec8da42f7aabe5c7deb5 --- /dev/null +++ b/data/part_6/1117663117.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"7d8a4b975e61a1951ea2270fc9b4052e","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/599a024d-ef97-40ef-a7f2-3e4a81d66577/retrieve","id":"-787943044"},"keywords":[],"sieverID":"6dc20cec-374f-4efe-a748-803fa1b606a9","pagecount":"140","content":"Kay ñankamuka wiñachishkami yanapak mashikuna papa lancha jawa allpapi llankakkunata jawalla yachachi tukuchun. Pishka yachana kamupimi chawpishka kay ñankamuka. Tukuy yachana kamukunami allpapi llankakkuna papa lancha jawa yachakchayachuntak rurashka kan. Kay yachaykunata tukuchishka washaka yachakuk mashikunaka kashnami llukshinkakuna:Riksinkami imashina unkuy rikurik kashkata shinallatak imami chay unkuyta rurakta (Yachana Kamu 1). Riksinkami imashina chay unkuy kawsayta charikta (Yachana Kamu 2). Riksinkami imalla papakunatak lanchapak sinchi mana sinchikunapish (Yachana Kamu 3). Fungicidakunawan papa jampi tukunkami lanchamanta jarkarinkapak (Yachana Kamu 4). Tarpushka papa chakrata rikurayashpa lancha unkuywan mana unkuchun sakinkachu, utkashpami jampikunata churay tukunka.Kashna shina ushaykunataka 2006 kallari watapimi kay Ecuador mamallaktapi allpapi llankakkuna, yanapakkuna, extesionistakuna shinallatak fitopatólogokunapish tantanakushpa yuyarishkakuna. Kipata, ñawpaman rurashka kamukunata japishpami kay ñankamutaka rurashka kan, chay ñawpaman rurashka kamukunaka kay mamallaktapimi rurashka Bolivia 1 , Ecuador 2 , 3 , Perú 4 shinallatak Salvador 5 . Kipataka kay ñankamukunataka alli mana alli kaktapish yanapak mashikunata shinallatak Escuela de de Campo de Agricultorespipish (ECAs) yachaykuna kushpami rikushka kan. Kay yachaykunataka 2006 kallari watapi rurarirka kay Ecuador mamallaktapi. Shinallatak Yachana Kamukunataka 2007 watapi, Perú mamallakta, Cajamarca villapi      * Ni tukurinmi sinchi jarkarik muyuka mana shuktak tukuchu tarpushka pachakunapi.PaPa lanchata jarKanata YachanKaPaK ñanKamuPhytophthora infestans (Mont.) de Bary manta shamushka lanchaka tukuy pachapimi papata unkuchishpa llakichikun. Ecuador mamallaktapika kay papa unkuytaka lancha shutiwanmi riksinchik chay unkuymi tarpushka papakunata chinkachishpa allpapi tarpuk mashikunamanka mana imata pukushpa llakichin. Centro Internacional de la papa (CIP), División de Manejo Integrado de Cultivos shinallatak Proyecto papa Andinawan tantarishpa; Instituto Nacional Autónomo de Investigaciones Agropecuarias del Ecuador (INIAP), Programa Nacional de Raíces y Tubérculos-rubro Papawan tantarishpa; shinallatak Secretaría Nacional de Ciencia y Tecnología del Ecuador (SENACYT), ayllullaktakunapi llakikunata rikushpa shinallatak imata ruranatapish mashkashpami kay yachana panka ñankamuta allichishkakuna: \"Papa lanchata jarkanata yachankapak ñankamu\" Kay ñankamuta rikukkunaka (paykunami punllanta rikukkuna kan) allpapi llankak mashikunata yachachikkunami kan, paykunamanka yanapak shutiwan riksinchik. Ashka kutinpi, yanapakkunaka allpapi llankak mashikuna kan paykunapak makikunawantak rurashpa yachashkakuna. Shinapish, kay ñankamuwanka técnico extensionista mashikunapish yachay tukunkuna ashtawankarin agropecuariomanta yachaya kuk jatun wasipi yachachikkunapish. Kay ñankamumanta yachakrikkunaka (paykunami kay ñankamupi tiyashka yachaykunataka japinkakuna) papata ashalla pukuchikkunami kanka. Shinapish, wakin yachaykunataka agronomía jawa yachakrikkunapish japi tukunkunami.Kay ñankamutaka rurashkami kan allpapi llankak mashikuna lanchamanta imashina jamutak mana jamuktakta rikushpa. Allpapi llankakkunaka papa lanchata jarkakkuna kachun shuyashpami kay ñankamuka rurashka kan. Jamuktak mana jamuktakta rikushpami yachana kamukunataka allichishka kan, yuyaypipish jawalla japinkapak, ruranapakpish jawalla kachun shinallatak tukuy yachaypi jawalla kachun. Chay tukuyta rikushpami paktaykunata churarkanchik imalla yachanamanta rikunkapak, imalla ruraykunata churankapak shinallatak yachashkata rikunkapakpish. Allpapi llankak mashikunapak yachay kashkamantami yanapak mashikunaka sumakta yachachina kan tarpushkakunapi llakikuna tiyakta mishachunkuna.Shuyanchikmi kay ñankamu maypipish minishtirik kachun shinallatak tukuy pachapi tiyak Centro Internacional de la Papaman kayshuk mashikunapakpish yanapay kashka kachun. shuk yachay tantanakuyta rurashpapish yanapak mashikunawan rikushkami kan. paktachiska kay Sierra Central del Ecuadorpi 2006 watapi. Yachanakunaka shinallatak japikatishkami kan yanapakkuna shuk yachana rurashkapi Cajamarca, Perú 2007 watapi. Shinallatak, ashtawan allichishka yachana kamukunataka inglés shimimanpish yallichishkanchik científico mashikunawan alli kakta mana alli kaktapish rikunkapak, kay yachay tantatankuyka Pyongyang (República Popular Democrática de Corea) pimi rurarirka.Kay ñankamupika achka yuyaykunami tiyan manarak yachay kallarikpimi rikush ka kana kan: Yachanapak rurashka. Kay ñankamutaka shuktak shuktak yachaykunapi yachashka kay tukun punllanta yachaykuna imapi, ashtawankarin ECAspi shinallatak ashalla yachaykunapipish. Ashka rikuchikkunatami charin yachaykunata yanapankapak (rikuchishpa, rikushpa, yuyarikuna, ruraykuna, rimanakuykuna, rurash yuyayta japikuna shinallatak kashka shina rurashpapish) shinallatak ñawpakman churan allpapi llankakkunapak yachashkakunata chaywan yachaykunata sinchiyachinkapak, chashna yachanakushpami papa lancha jarkarinamanta ashtawan yachashpa llukshishun. Chaymantami, yanapak mashika chashna yachaykunata ñawpakman churakmi kana kan mana paylla yachashka shinachu kana kan. Allita rikuchin. Kay ñankamuka lancha ima kakta mana yalliktakka rimakunchu ashawan yachashun nishpaka shuktak killkakunapi shinallatak kamukunapimi shinallatak killkashka uchilla pankakunapipish 6 rikuy tukunchik. Kay ñankamuka lancha papata ama japichun minishtirishka yachaykunallatami rikuchikun. Shimikuna. Kay ñankamuta yachankapak ama skinchi shimikuna churankapakka wakin rimaykunataka jamutanalla shimikunapimi killkashka kan. Churashunlla Phytohthora infestans shimita killkakunkapak rantika Fitóftora shutiwan churashka kan killka katinapak shinallatak rimaypakpish jawalla kachun. Shinallatakmi micelio shimipak rantika pelusilla shutita churashkanchik chay lancha wiñarichun yanapakkunataka. Chayshinallatak P. infestans omomiceto nishkataka hongo shutita churashkanchik hongoman rikchak kashkamanta. Imalla ministirin kay ñankamukata yachankapak. Kay ñankamuta yachachikkunaka killkana shinallatak ñawirinatapish yachakmi kan kan. Ashtawanka, papata tarpuk mashikuna kana kan shinallatak yachaykunapipish tantanakushpa yachana munayta charikkuna. Yachakukkunaka killkana ñawirinatapish mana yachakkunapish kay tukunmi.     6. Pérez W. shinallatak Forbes, G 2007. Lanchata jarkankapak chikan chikan llankaykuna. Willaykunata yallichik uchilla pankakuna 1 manta 6 kama: 1. ¿Imatak kan lancha?; 2. ¿Imatak kan lanchata jarkankapak chikan chikan llankaykuna?; 3.¿Imatak kan sinchikay rikchayachiy?; 4. ¿Imatak kan plaguicidakuna?; 5. ¿Imatak kan fungicida?; 6. ¿Imapak bomba de mochilata yaku pakta yallichun allichinarak kan? Centro Internacional de la Papa (CIP). Lima -Perú.Kay ñankamuta yachachikrikkuna. Kay ñankamumanta yachachikrikkunaka kamupi nikushkata manarak yachachi kallarikpi paykunarak yachayta chaskina kan. Kay yachaykunaka ishkay mana kashpaka kimsa punllakunatami ruray tukunkuna lancha unkuy ama japichun jarkarikunamanta yachak mashikunawan shinallatak imashina kay kamu rurarishkata yachak mashiwanpish. Maypi yachachina. Kay ñankamutaka rikushka shinallatak yachashka kana kan maypi kawsakta rikushpa shinallatak imami tarpukkunata rikushpapish, ashtawankarin Ecuador mamallaktamanta kanllaman yachachishun nishpa. Churashunlla Tantachiska 2 fungicidakunapak shutikuna jawa, mana kashpaka shuyukuna, rimaykuna shinallatak rikuchikakunapish rikushkami kana kan kay shukktak mamallaktakunapi kay ñankamumanta yachachinkapakka.Llika. Tukuy yachana kamukunapi kashna churashka kan: Kay ñankamutaka papa lanchata imashina jarkanamanta yachaykunallapimi rikushka kana kan. Asha yachaykuna tiyanmi shuktak murukunapi lanchamanta jarkanapak, tomatemanta rimashpa. Shinapish, chashna ima kashpapish ministirishkashina churashkami kana kan. Yachana Kamu 1 manta 5 kamaka washanmi yachashka kanata charin. ECA yachaykunapika imashinami tarpushkakuna wiñarimukpi yachana kamukunataka yachashpa katinata charinkuna. Mana sinchi muyukunata tarpushka kakpi shinallatak ashka tamya kay pachakuna imapika utkami fungicidakunata churashka kana kan manarak Yachana Kamu 4pi yachachikushkata rikushparak. Shina kakpika, yachakukkunamanka ninami tukun fungicidakunamantaka kipatami allita riksishun shinapish kay jampikunata churaykunaka ministirinmi lanchata jarkankapak. Yachana 3 pi sinchi muyukunata churanamanta yachachikun chay yachaytaka shuk kutinwan tarpushun nishpaka tikra yachana kan. Pachakuna Kay yachanaka ishkaypimi chawpirin, kallarik tantanakuyka kimsa pachakuna shinallami kan, katik tantanakuyka kanchis punllakunata rurana, kimsa chunka tatakikuna yachaykunata rurana.Kallari rimay Lanchataka shuktak papa unkuykunami yuyashpa mana utka rikunchiklla chaymantami minishtirin alli riksina imashina chay lancha japik kashkata shinallatak yachana chayka fitóftora nishka hongomanta japik kashkata. Chay hongoka papa yurakunapimi rikurinlla kutakunata churashka laya chaymi ashka llakichik uchilla kurukunaka waranka waranka tukun.Paktaykuna Kay yachay pankata tukuchishka kipaka yachakukkunaka kaykunatami ña ruray tukunkuna: Tantanakushka mashikunapak ñawpakpi shayarishpa imashina lancha japik kashkata rikuchinata ushankami chashnallatak shuk rikuchinatapish ninkallami runamanta unkuymanta. Imashina papa pankakunapi, yuyukunapi, papa muyukunapipish chay lancha unkuykuna japik kashkata shinallatak sapikunapi kayshuk unkuykunami yuyashpa mana pantarinkachu. Shuyushpa rikuchinatapish ushankaami lancha ima kashkata shinallatak chayka ima rurak kashkatapish rimankallami.Tantarishka mashikunaman papa pankakunapi chay kuta churashka shina rikurik kurukuna ima nisha nishkata shinallatak imata rurak kashkatapish riksichi tukunkunami 1.3.Allimanta kay killkata rikuna kan. 1, 2, 3 yachayruraypi nishka shina imakunata mashkana kan. Killkashka pankakunapak rikchata llukchina kan, chay rikchakunataka tullpuwan llukchina kay pankakunamanta 20, 21 shinallatak 27 (tukuy yachakukkunami rikcha pankakunata charina kan). Killkashka pankakunapak yurak yanapi llukchishka rikchata charina kan kay pankakunamanta 33, 34 shinallatak 36 (Tukuy yachakukkunami rikcha pankakunata charina kan). Rurasha nishpalla kaytaka rurana. Yachashkata rikuna minishtirishpaka manarak yachaykuna kallarikpi shinallatak yachay tukuchipi yachashkata rikunapak pankakunata allichinami (panka 39 shinallatak tantachishka 1). Rurashpatak yachankapakka pakalla pushtutami mashkana kan (shuyukunata rurankapak, microscopiowan rikunkapak, shutuklla cajakunata rurankapak imapish) shinallatak papata lanchata katichishpa yachay tukun.1.5.Yachana Kamu 1: Papata imashina lancha japik kashkata shinallatak hongo Fitóftora imashina rikurik kashkatapish riksishunchik Yachana Kamu 1 Kunan yachaypika yachakukkunawanka kay llankaykunatami rurana kan Tantanakuy 1Kay yachay rikuytaka ruranatakmi. Yachakukkunamanka kay tapuykunata rurana kan kay rimaykunata kushilla kallarinkapak, paykuna yachashkata japinkapak shinallatak chayta rurashpaka mashna yachakkuna kashkata yachankapak. ¿Kikinkunamantaka pitatak uju japishka? ¿Imatak kan unkuy? ¿Imatak kan unkuy rikuri? ¿Mashnakunatatak papata lancha japishpa llakichishka? ¿Pantarishkankichikchu shuktak unkuymi nishpa? ¿Imallatak rikurin pankakunapi, yuyupi, papa muyupi? ¿Kankunaka imata yuyankichik imamantatak lancha japinka? ¿Imatak kutakunata shina papa pankapi rikurishpa? ¿Chayka imatatak ruran? Rayawan kak tapuykunata kutichishkakunaka shuk jatun pankapi killkashka kana kan. Kay yachana kamupi yuyaykunata killkashka shina (37 pankapi rikuy) chimpapurashpa rikuna kan yachakukkunapak yachashkakunawanpish chaypimi rikurinka wakin yachaykunaka riksishka shinapish shuk yachaykunaka mushuk kashkata rikuchinami. Rurasha nishpallami kay yachay rikuchitaka rurana kan. Yachakukkuna mashna yachashkataka yachankapanka 39 pankapi killkashkata kutichishpami yachana kan chayllatatakmi yachay kamuta ña tukuchishpapish rurana kan. Imashina ruranamantaka tantachishkata 1pi rikuna kan. Yachakukkuna kay kamumanta imata shuyankunata yachankapakka tapuykunatami rurana kan, churashunlla kay tapuyta ruray tukunkichik ¿kunan punllaka imapak tantanakushkanchik? Kipami yachakuk mashikunaka kay kamupi imata kachakrishkataka yachak chayana kan, jatun pankakunapi killkashpa rikuchi tukunkichik.1 shinallatak 2 tantanakuyta rurankapakka mashna pachakuna minishtirishkatarak rikuna kan. Chashnallatak achiktami sakina kan ima jawa mana rimakrishkata, churashunlla:Papapak shuktak unkuykuna. Papaman ama lancha japichun jarkaykuna.Yachana Kamu 1 Yachayruray 1. Papata pankakunapi, yurapi shinallatak muyupipish lanchata japik kashkata yachashun Paktaykuna Kay rurayta rurashka washa yachakuk mashikunaka kayta rurankapak yachashka sakirinkakuna: Tantanakushka mashikunapak ñawpakpi shayarishpa imashina lancha japik kashkata rikuchinata ushankami chashnallatak shuk rikuchinatapish ninkallami runapak unkuymanta. Imashina papa pankakunapi, yuyukunapi, papa muyukunapipish chay lancha unkuykuna japik kashkata shinallatak sapikunapi kayshuk unkuykunami yuyashpa mana pantarinkachu.1.Minishtirinmi Kunanlla papata lancha japikukta shinallatak papa shuktak unkuywan kaktapish charina (Mirachishka yuyay 1). Charishpaka sapan yachakukkunami lupata charina kan. Shuyunkapak imakuna: killkana pankakuna shinallatak shuyuna killkana kaspikuna. Shuyushpa mishanakuypak kunpitaytapish charinami.Imamanta kay rurayta rurakrishkata yachakukkunaman willanami. Chusku mana kakpika pichka mashikuna pura tantachinami. Yachakuk mashikunaman muestrakunata, lupa shinallatak imalla minishtirishkata kunami. Yachakuk mashikunata mañanami muestrakuna akllachun papa ima unkuywan kakta rikushpa amamanta chashna akllashkata willana kan. Minishtirishpatakkarin amamanta chashna unkuy japik kashkata willanata charinkuna. Yachakukkuna papa lanchata shinallatak yurak kutakunata shina papa pankakunapi kak kurukunata rikuy tukuchun yanapanami.1.3.Tukuykuna tantanakushpa parlanakuna kan imatak kan unkuy rikuri, imamantatak papaman lancha japin pankakunapi, yuyupi shinallatak muyupipish. Yachakuk mashikunaman mañanami papa lancha imashina rikurikta shuyuchun, kunpitay tiyanmi nishpa willana kan kay yachay 2 rurashkamanta. 6.20, 21 pankakunapi killkashkakunapak rikchata 8. llukchishpa yachakuk mashikunamanka kuna chay killkakunata kipa punllakunapi ñawirishpa ashitawan achiklla yachaykuna kachun.Papa tarpushkata mana charishpa imaka maypi papa chakra tiyashkapii rikunaman rina kan yachakukkuna papa lanchakunata tantashpa apamuchunkuna. Manatak chakra tiyakpika yanapakmi maymanta kakpipish rukuchita apamuna kan llankayta rurankapak. Chay rikuchitaka tutamantakunatami tantana kan sumak waylla pankakuna kachun. Ama chakirichunka refrigeradorapimi plastiku fundakunapi churashpa wakichina kan.Yachana Kamu 1Tizón tardíoka papata japik shuk unkuymi kan chay unkuyka shuk uchilla kurukuna shina pankakunata, yuyuta shinallatak papapak muyutapish llakichinmi, chay unkuytaka papa lancha shutiwanmi tukuykuna riksinchik.Uchilla kurukuna Uchilla kurukunaka kawsayta charikkuna kan shinapish mana jawalla rikuypak (Mirachishka yuyay 2 rikuy). Wakin kurukunaka runaman yanapakkuna tiyan. Churashunlla allpapi kawsak kurukunaka wanuta ismuchinkuna. Shinallatak tiyanmi uchilla kurukuna unkuykunata kuk kurukuna. Churashunlla papa lancha, runaman gripeta kuk kurukuna, mana kashpaka warmi wakrapa ñuñupi japik mastitis nishkata kuk kurukuna imapish.Unkuy Runata kashpa, wiwata kashpa, ima shuktak kawsayta charikta kashpa imapish unkuykunaka chayanmi shuk uchilla mana rikiuypak kurukunawan shinallatak shuktak shinapish japi tukunmi: Uchilla kurukunawan japishka unkuykunaka kanmi papa lancha, runaman gripeta kuk kurukuna, mana kashpaka warmi wakrapa ñuñupi japik mastitis nishka imapish. Shutak shina japik unkuykuna kanmi papapi papapi nitrógeno illaymanta, mana alli mikuyta runa mikukpi wiksa kachari, wiwakunapi millma urmay minerales illaymanta.Unkuyta rikuchik Kawsayta charikkunaka ima nanaytapish rikuchin. Papa yuramanta rimakpika yanayashpa rikurin lancha japishka kakpika, runamanka gripe charishpaka umata nanan, warmi wakrakunapikka ñuñumanta razutiklla shukllin ñuñu unkushka kashpaka.Kay yuyachi alli Kankan karka unkuy ima kashkata ashtawan riksichinkapak shinapish mana yalli minishtirishkachu kan unkuyman ashtawan yachana.¿Imatak unkuy rikuchika? Kawsayta charikkunaka unkuywan kashpaka imalayapish rikuchinmi. Nishunlla papa yuraka yana manchata rikuchin lancha japishka kashpaka.Kay pankataka yachakukkunaman kuna kan manchakuna shina muyurishka rikurin. Wakinpika chay yanayashpaka muyuntikpika asha killuwan wayllawan rikurin.Yurapi unkuy rikuri: yana shinaman chakishka manchakuna rikurin. Yuraka chay yanayashkapi uturi tukunmi.Muyupi unkuy rikuri: Papa muyupi yana shinaman manchakuna rikurin, ismukuk shina kan. Papata chawpishpa rikukpika chakishka manchakuna shina yanalla rikurin. Papa muyu lanchawan kakpika mana imata ashnachinchu.Papa chakra lanchawan kashpaka ismuska pankaman ashnachin.Yachana Kamu 1Papa lanchawan kashpa ama shukta unkuykunawan pantarishunPapata kasa japishka kashpaka pankakunaka asha yanayashka chakishka shinallatak pakirikuk rikurin ashtawan yallika jawaman kak pankakunapi.Papa lanchaka muyurishkapi yanawan killuwan rikurin. Chay unkuywan kak pankaka killuyari tukunmi.Fomosis nishka unkuyka rikuchinmi uchilla manchakunata shinami paykunapura tantari tukunmi pankata ashawan yanayachinkapak.Septoriosis nishkaka uchilla manchakuna ashawan uchilla yana puntukunata rikuchin. Chay manchakunapak muyuntikpika killuyashka rikuri tuyunmi.Foto: CIPYachayruray 2. Papa lanchata kuk hongo kuruta riksishun Paktaykuna Kay yachayta tukuchishpaka yachakuk mashikunaka ruray tukunkakunami: Shuyushpa rikuchinkakunami imami papaman lanchata kuk kashkata shinallatak willankami imashina papa lanchawan kakta yachanamantapish. Fitóftora uchilla kurukuna ima kashkata shinallatak imata rurakkuna kashkatapish willay tukunkakunallami tantarishka mashikunapak ñawpakpi.1.Shuklla microscopio imapish (Mirachishka yuyaykuna 3). Microscopiopa vidriomanta placakuna (shuk placa sapan microscopiopa minishtirinmi). Cinta adhesiva transparente. Yaku. Algodón, goma shinallatak kutatapish minishtinchikmi espora fitóftorakunata rikuchinkpak.Yachakuk mashikunaman willana kay ruraywan imatami shuyanchik. Ñawpa punllakunapi tantarik mashikunallatak tantarinata charinkuna paykunaman imalla minishtirishkata kuna kan. Esporas ima kashkata yachankapak shuk rikuchita rurana kan kashna rurashpa: Vidrio placa jawapi shuk gota yakuta churana.1.3.Asha cinta adhesivata pitina shinallatak allimantalla papa pankapi kutakuna shina tiyakuk jawapi cinta adhesivawan chay kutakuna shinata citapi pigachina.Vidriopi shuk gota yaku churashka jawapi cintata churana. Mana yalli aysana.Yalli yaku kakpika papel higiénicowan chakichina. Kay rimaywanka microbio uchilla kurukuna tarpushkakunata llakichik kurukunata riksinchik. Kay kurukunataka mana jawalla rikuy tukunchikchu shinapish waranka waranka tantarishka kashpaka rikuy tukunchikllami (Mirachishka yuyay 4).Yurak kutawan shinallatak pushkawan shina pankakunapi muyurishka rikurin shinallatak tamyalla tuta mana yalli chiri pakarikpika yurakunapipish rikuri tukunmi. Chay kuruka waranka waranka esporakunawan pushkakunapi pillurishka shina kan. Yurak kuta pushkawan shina pelusilla kakpika papa lancha kanka chashnami shuktak unkuykunawan mana pantari tukunchik. Fitóftorakunamanta esporakunaka limónkuna shina kan.. Signo rimayka wakin yachakuk mashikunaka pantari tukunmi chaypak rantika \"pelusilla'','' algondocilla'', ''lanilla'' shutiwan yachachi tukunchik. Yachakuk mashikunaman willana kay ruraywan imatami shuyanchik. Shutuk caja ima kashkata shinallatak imapak kashkatapish willana. Kay ruraytaka shuk shuk runana kan. Minishtirishkakunatapish tukuykunaman kuna kan.1.2.Panka 33 ta rikcha llukchishpa tukuy yachakukkunaman kuna. Shutuk cajata imashina ruranata rikuchina. Sapan yachakukkunaman mañana ishki shutuk cajakunata rurachun. Sapan shutuk cajapi kimsa mana kashpaka chusku alli papa pankata uri sinkaman churana.Punta rurashka shutuk cajapika tawka gota yakuta uri singa churashka panka jawapi churana, chayta rurana kanchik mondadienteswan.4.6.Cajata tapashpa killkana kanchik \"Yaku\" killkata. 7. Shuk rikuchita rurana kanchik pelusillata mayllankapak, mayllashka washaka esporakuna tukuchun: 10 mana kashpaka 15 lanchawan kak pankakunata japishpa pitina kanchik pelusillawan kakta. Chay pitishka pankakunatami plástico vasopi churana ashalla yakuwan churashka pankakunata tapachunllami yakutaka churana. 8. Paktaykunaman chayankapakka tantanakuy 1 tukuchishpa kay kamupi imalla yachashkakunata tikra yuyarishun, kay yachaykunami karka: Imashina unkuy rikurik kashkamanta. Papa lancha imashina rikurik kashka. Imamantatak lancha japik kashka shinallatak imashina chay unkuyta riksi tukunchik shuk papa yurapi. Fitóftora ima kashka shinallatak imata rurak kashkatapish.Kayta rurankapakka yachakukkunaman rikchata llukchishka kushka pankakunawan rimay tukunchik. Kay pachapika rikuna kanchik kay yachay kamuta manarak yachay kallarishpa imatami yachakuk mashikunaka wakin tapuykunata kutichishkakuna, chimpapurashpa rikunkapak kay kamuta ña tukuchikukpi ashawan yachashka mana yachashkata rikunkapak. Shinallatak rikuy tukunchik yanapak mashi killkashkakuna tantanakuykunapi rimarishkakunatapish.Tantanakuy 2 tukuchishka washa tikra yuyari tukunchik imalla yachashkakunata shutuk cajakunata rurashka washa. Yachakukkunaka achik sakirina kan Fitóftoramanta esporakunawan papa lancha japik kashkata yachaspa. Kay yachayta rikuytaka ruranatakmi: Yachaykuna paktashkatak rikunapakka chay pacha tawka yachakukkunata kay llankaykunata rurachun mañana kanchik: Tukuykunapak ñawpakpi síntoma ima kashkata rimachun shinallatak shuk rikuchita churachun runapak unkuy síntoma jawa. Papa lancha imashina rikurin pankakunapi, yuyupi, muyupipish shinallatak imashina mana shuktak unkuykunawan pantarinamantapish rimachun. Shuyushpa rikuchina imashina lancha rikurik kashkata, pitik unkuchik kashkata shinallatak imashina papa yurapi riksi tukunchik jawa rimachun.1.3.Tukuykunapak ñawpakpi fitóftoramanta esporakuna ima kashkata shinallatak imata rurak kashkatapish willachun mañanami.Rurasha nishpallami kay yachayta rikuchitaka rurana. Yachashkata ashtawan rikusha nishpami kay tapuykunata kutichichun mañana kanchik, kay tapuykunaka Panka 39pi killkashka tiyakun, imashina ruranataka Tantachishka 1pimi willakun. Chay tapuykunata kutichishka pachallatakmi alli kutichishkata rikunachina kan shinallatak yachakukkunawan parlanakuyta rurana 4.Kay yachay kamu imashina rikurishka jawa yachakukkunata kay tapuykunata rurana kanchik: ¿Papa lancha imashina rikurik kashkata yachankapakka minishtirirkachun shuyushpa rikuchina? ¿Lupakuna shinallatak microscopio yanaparkachu yachaykunapi? ¿Fitóftoramanta esporakuna papata lancha japik kashkata yachankapak allichu karka shutuk cajakunata rurana? ¿Ima llakikunatatak charirkanchik? ¿ Yachankapak rurankapakpish pachakunata paktachu karka? Kallari rimay Papata ama lancha japichunka imashina lancha rikurik kashkata, maymanta lancha shamuk kashkata yachana kanchik shinallatak chay kurukuna maypi wiñak kashkata yachana kanchik. Kay yachaykunamantami kay kamupika rimashun.Ruray 1: Imashina lancha rikurik kashka maymanta lancha shamuk kashka shinallatak chay kurukuna maypi wiñak kashkatapish rurashun.Paktaykuna Kay kamuta tukuchishka washaka yachakukkunaka shuyushpa rikuchi tukunkakuna imashina lancha rikurik kashkata, maymanta lancha shamuk kashkata shinallatak chay kurukuna maypi wiñak kashkatapish.Allimanta kay killkata rikuna kan. Yachayruray 1pi willakushka shina imalla minishtirishkata charina kanchik. Panka 53 rikchata llukchina kan (tukuy yachakukkunami rikcha pankakunata charina kan). Rurasha nishpalla kaytaka rurana. Yachashkata rikuna minishtirishpaka manarak yachaykuna kallarikpi shinallatak yachay tukuchipi yachashkata rikunapak pankakunata allichinami (panka 55 shinallatak Tantachishka 1). Yachayruray 1 pi imashina rurashkata tikra rikuna. Imashina lancha rikurik kashkata, maymanta lancha shamuk kashkata shinallatak chay kurukuna maypi wiñak kashkamantapish alli yachana kanchik. Rurashpatak yachankapakka pakalla pushtutami mashkana kan (imashina lancha rikurik kashkata shuyunkapak) shinallatak ushashpa imakarin papa chakra lanchawan kakta charina kanchik.1. 2.4.6.Yachana Kamu 2Kay rimanakuypakka Yachana kamuu 1 kay yachaykuna jawa killkakunamanta rikchata kushka killkata rikuy tukunchik. Kay yachayta rikuytaka ruranatakmi. Yachakukkunamanka kay tapuykunami rurana kan kay rimaykunata kushilla kallarinkapak, paykuna yachashkata japinkapak shinallatak chayta rurashpaka mashna yachak kashkata yachankapak. ¿Fitóftora maypi kawsan? ¿Imashinatak mirankuna? ¿Lanchaka imashinatak wiñan? (Mirachishka yuyay 1). ¿Fitóftoraka maypitak kawsanata munan? (Mirachishka yuyay 2). ¿Tamya shinallatak kunuk pachaka imashinatak yanapankuna lancha wiñachunka?Kay tapuykunata kutichishkakunaka shuk jatun pankapimi killkashka kana kan. Kay yachana kamupi yuyaykunata killkashka shina (54 pankapi rikuy) chimpapurashpa rikuna kan yachakukkunapak yachashkakunawanpish chaypimi rikurinka wakin yachaykunaka riksishka shinapish shuk yachaykunaka mushuk kashkata rikurinka chay yachaykuna mushuk kashkata yachakuk mashikunata rikuchina kanchik. Rurasha nishpallami kay yachayta rikuchitaka rurana. Yachakukkuna mashna yachashkataka yachankapakka 55 pankapi killkashkata kutichishpami yachana kan chayllatatakmi yachay kamuta ña tukuchishpapish rurana kan. Imashina ruranamantaka tantachishkata 1pi rikuna kan.Yachakukkuna kay kamumanta imata shuyankunata yachankapakka tapuykunatami rurana kan, churashunlla kay tapuyta ruray tukunkichik ¿kunan punllaka imapak tantanakushkanchik?kipami yachakuk mashikunaka kay kamupi imata kachakrishkataka yachak chayana kan, jatun pankakunapi killkashpa rikuchi tukunkichik mana kashpaka tarjetakunapipish killkay tukunchik.Manarak yachay kallarikpi mashna pachakuna minishtishkata rikuna kan, ashtawanpish wakin rimaykuna jawa mana yacharishkatapish achikta willana kan, churashunlla:Shuktak unkuykuna imashina wiñak kashka jawa. Lancha ama japichun jarkaykunamanta.Lanchapak ciclota alli jamutachinkapakka kayta rimay tukunchik: mashi Juanka kashnami: taytamamaka yunka llaktamantami shamushkakuna kan, paykunaka 25 watakunata charishpa sawarirkakuna chay washami Juantaka kay pachaman apamushkakuna. Juanka Guaranda villapi wacharishka wiñashkapi, 30 watapimi sawarishka kunanka ishki wawakunatami charin.Kay Ecuador mama llaktapi papata tarpukkunaka tizón tardíotaka lancha shimiwan riksinkuna chaymanta yachakukkunaman lancha shimiwan rimashpa yachachina tukunchikllami.Mirachishka yuyay 1 Kay yachay kamu imashina rikurishka jawa yachakukkunata kay tapuykunata rurana kanchik: ¿Imapaktak lancha imashina kallari-tukrinta yachana alli kashka? ¿Ima llakikuna tiyashka? ¿Rurankapakka pachakuna paktachu karka? Manarak tantanakuyta kallarishpa yanapak allichirinapakYachana 1ta shinallatak Yachana 2tapish riksina kanchi.Pachakuna Ishkay pachakunata minishtinchik shuk tantanakuyta rurankapak.Kallari rimay Lanchata jarkanata yuyashpaka alli sinchi papakunata tarpushpallami jarkay tukunchik. Kay yuyay, shinallatak shuktakkunata ñawpakman yachashpami, ashka alli kan ima papakunata tarpunata yuyarina pachakunapi. Kay yachana kamupika shuk ruranapishpimi tiyan yachakukkuna papakuna lanchamanta jarkarinamanta shuktak yuyaykunata riksishun munashpaka ruray tukunllami.Paktaykuna Yachakukka kay yachanata tukuchispaka ushankami: Shuyukunapi sinchi muyuna shinallatak mana sinchi muyukuna ima kashkata rikuchinata ushankami, willay tukunkunapish imashina kimsa shuktak shina kakta shinallatak imamanta sinchi muyukunata charinata.Llaktapi tiyak papakunapak shutikuna killkashpa rikuna kanchik mayjanlla papakunata lancha japik kakta mana japik kaktapish. Rurasha nishpaka kayta ruray. Tantanakushkapak ñawpakpi rikuchina imamantatak sinchi lanchamanta jarkari muyukuna kakta, shinallatak imamanta kipataka mana sinchi kay tukun, ruranapi rikushpa ruray tukunkillami.1.3.Allimanta kay killkata rikuna kan. Kay yachana kamupi yuyaykunata killkashka shina (77 pankapi rikuy) chimpapurashpa rikuna kan yachakukkunapak yachashkakunawanpish chaypimi rikurinka wakin yachaykunaka riksishka shinapish shuk yachaykunaka mushuk kashkata rikurinka chay yachaykuna mushuk kashkata yachakuk mashikunata rikuchina kanchik.Yachakukkuna yachashkataka japitukunchikmi panka 79 manta tapuykunawan, chayllatatakmi yachanata tukuchiska kipaka tapuna. Rikuchik allichishakaka Tantachishka 1 pi churashka.Yachakukkuna imata kay yachanamanta shuyashkata yachankapakka kay tapuykunata churay tukun: ¿imapakmi kunan punllaka tantanakunchik? Kipami yachakuk mashikunaka kay kamupi imata kachakrishkataka yachak chayana kan, jatun pankakunapi killkashpa rikuchi tukunkichik mana kashpaka tarjetakunapipish killkay tukunchik.Minishtirintakmi kay yachanapi mana rimanakunataka mana rikushunchu nishpa, churashunlla:Químicowan imashina lanchata karkanata.Ninkunami shuk muyuka \"yaykushka kanmi\" shuk Centro de Investigación mana kashpaka shuktak institucionkuna tarpukunapak makipi churakpi, mana kashpaka chayshuk tarpushkakunamanta apamushka kashpapish.Yachana Kamu 3 Mirachishka yuyay 1. Yachashun imatak kan sinchi muyukuna shinallatak mana sinchikunapish Paktaykuna Kay rurayta tukuchishka kipaka yachakukkunaka ushankakunami shuk shuyupi rikuchinata ima sinchi muyuna kashkata shinallatak mana sinchi muyukuna kaktapish, kimsa shina chikanyachikkunatapish rikuchishpa shinallatak ama lancha japichun mayjanlla papa muyukunata tarpunamantapish riksichi tukunkakunami.Fitóftoramanta pelusillata rikuchinkapakka algodón shinallatak gomata minishtinchik. Uchilla yurak papel muyuchishkakuna mana kashpaka espuma sintética lancha pillurishka kakta rikchinkapak. Puka marcadorkuna.3 jatun cartulinakuna (70 x 50 cm) shuktak shuktak colorkuna (2 wayllakuna shinallatak shuk ankas). Kaykunatami shuyuna: 2 papa yurakunata 2 waylla cartulinakunapi. Tamyataka ankas cartulinapi.Imashina rurana Kay ruraytaka ruray tukurinmi lancha japishka papa tarpushkapi shinallatak rikushkami imashina lancha kallarin shinallatak imashina lancha tukurin (Yachana kamu 2). Imamanta kay rurayta rurakrishkata yachakuk mashikunaman willanami. Chusku mashikunata akllana paykunaka lancha tukuchun, shukka papa yura kachun shuk alli muyumanta wiñashka, shuktak yuraka mana sinchi papamanta wiñashka shinallatak shukka tamya. Lanchata rikuchikuk mashiman kuna yurak papel pilluchiskakunata mana kashpaka espuma sintéticatapish (rikuchinkami lanchata apakta) shinallatak algodónka (unkuyta wiñachikta).1. Papa yurakunata shinallatak tamyata rikuchik mashikunamanka cartulinakunata papata shinallatak tamyata shuyushka cartulinata kuna kan. 4. Tantanakushpa yachakukkunawan rimanakuna imalla rimaykunata rikushkata paykunallawantak nina imatak sinchi muyuna kashkata shinallatak mana sinchi muyukuna kaktapish, kimsa shina chikanyachikkunatapish rikuchishpa shinallatak ama lancha japichun mayjanlla papa muyukunata tarpunamantapish. 5. Shuk rikchata kuna panka 66 tukuy yachakukkunaman ima kashkata achikyachinkapak shinallatak ima kashkakunatapish ama kunkarichunkuna.Yachashun imatak sinchi muyukuna shinallatak mana sinchikunapishTamyakuy pachakunapi ashallata unkuk muyukunami, shinaka pukunatami charin.Tamyakuy pachakunapi ashkata unkukkunami shinaka papaka mana ashka pukuy tukunmi.Ashalla lancha yuraman yaykurin Ashka lancha yuraman yaykurin Allimanta manchakunaka wiñan Manchakuna utkami wiñarin Ashalla pelusilla tukun papa yurapi Ashka unkuyta wiñachikkuna tiyarin ¿Ima allikunatak tiyanka shinallatak ima mana allikuna? Mana ashka fungicidata minishtin Ashalla kullkiwan ashalla pachakunapi fukuy tukun. Wayratapish ashallata mapayachin. Kausayta ashallata wakllichin. arpushkaka tamya wata kakpipish mana chinkarinkach. Yachana Kamu 3Paktashpaka kay llankayta rurana Yachayruray 3. Yachashun imanishpatak mayjan muyukunaka lancha japishka kakpipish mana unkurin, kipataka jawalla unkuriklla kashkamanta Paktay Kay rurayta tukuchiska washaka yachakukkunaka ushankakunami tantanakushkakunapak ñawpakpi rikchinata ima muyu jarkarik kashkata shinallatak imamantatak kutin kipataka ña mana jarkari tukun, ruranapi rikuchishkakunata katishpa.Shuk plancha de espuma sintética. 5 yurak aujakuna shinallatak 5 puka aujakuna. Shuk candado paypak llavewan shinallatak 4 shuktak llavekunatik. 20 uchilla waylla cartulina tarjetakuna (2 x 2 cm). Shuk papa yurata shuyuna chay tarjetakunapi.Ima yuyaywan kay rurayta rurakushkata yachakkukunaman willana.3 mana kashpaka 4 papa yurakuna tarjetakunapi shuyushkata plancha de espuma sintéticapi jawapi churana. Kay muyukuna rikuchinmi kunanlla apamushka papakunata chaymantami ashalla tiyan manarak achka muyukuna tiyachu nishpa yachakukkunaman willana. Yachakukunaman kuna yurak aujakunata chaymantaka nina rikuchinmi lanchata apakta, mana ñukanchik muyukunawan tarpushkata llakichinka. Yuyarina chay lanchata apakka shukta papa yurakunamantami shamun, shuktak yuritakunamanta shinallatak chay unkuywan unkushka sachakunamantapish.Yachakukkunata mañana yura pankakunapi shitachun chay aujakunata. Chay auja mana pankaman chayashka kashpaka rikuchinmi lanchata apakka pampaman urmashpa wañushkata.1.3.Shuktak auja pankata japikpika ninami kay yuraka mana lanchawan unkunkachualli sinchi jarkarik muyumanta wiñashka kashpa. Rikuchina chay jarkariklla muyuka imamantatak mana unkun, mañana shuk mashita chay yurata rikuchik kachun shinallatak shuk wishkashka candadota kuna. Mayjanmi chay aujawan shitakmanka kuna shuk mana alli llaveta chaymantaka paskay nina. Muyuka sinchi jarkariklla kashkamantaka, candadoka (yurakunapak jarkarikta rikuchik) mana alli llavewanka mana paskarik tukun (chayka rikuchinmi lanchaka mana jarkashkakuna mishashpa yaykuy tukuk kashkata). Chay muyupi lancha kawsankapak imashinapish tukunami kan. Chayta rurankapakka yurak aujatami puka aujawan cambiana tukun. Puka aujakunaka rikuchinmi jarkarishka yurakunata mishay tukushkata, chaymanta paskashpa yaykuri tukun unkuykunantik. Llutana 5 mana kashpaka 6 mushuk yurakunata chay espuma sintética jawapi shinallatak nina yurakunaka mirarishkami tarpukkuna munashpa tarpushkamanta, shinallatak ashka allpapi tarpushkakuna. Kutinllatak yanapakkunata mañana aujakunata shitachun. Puka aujakunata charikuk mashi aujawan pankapi shitashpa paktapika ninami chay yuraka lanchawan unkunmi. Rikuchina kanchik shuklla aujawan ashalla pankakuna tiyakpi mana jawallachu, achka pankakuna tiyakllapimi jawalla unkuchinka puka aujakunaka. Imamanta chay yura unkuk kashkata rikuchinkapak, wishkashka candadota kuna yurata rikuchikman. Mayjanmi puka aujawan paktarka pay mashiman kuna chay candadopak llaveta jawalla paskay tukuchun.7.8.10.12.Nina tukun lanchata apakkunaka puka aujakuna shinami kanka paykunallami kawsay tukunka. Yura aujakunapak rantika puka aujakunatami churana. Llutana 5 mana kashpaka 6 mushuk yurakunata espuma sintética jawapi shinallaak nina yurakunaka mirarishpami katin tarpukkuna munashpa chay sinchi muyuta tarpushkamanta. Kutinllatak yachakkunata mañana kutin aujakunata shinatachunkuna. Nina shinallatak ashka yurakunami jawalla rikushka kay tukun chay jutkukkunawan. Rikuchina shinallatak ña chay muyukunaka mana sinchi jarkari tukuk kashkata. Tantakushpa rimanakuna imalla rimashkakunamanta shinallatak ninakuna imata jarkarik muyu kashkata shinallatak imamanta ña mana sinchi kay tukun. Rikchakunata kuna panka 76 tukuy yachakukkunaman shinallatak yachashkata tikra rikushpa ama kunkarichunkuna.13.14.16. 17.Kaytaka rurasha nishpalla rurana ¿Imatak sinchi muyu?Mana jawalla lanchawan unkurin. Lancha japikpika, lanchata apakkuna mana yuraman yaykuy tukunkuna shinallatak manchakunapish mana wiñarin, lanchata wiñachikpish mana wiñarin. Chay muyukunaka mana fungicidakunata minishtinchu, ama kipata japichun nishpaka churanallami. Chaymanta jamutankapakka yuyarina tukumun chay muyuka candadowan wishkashka shina kakta shinapish lanchata apakkunaka mana llaveta pasknakapak charinkunachu. Chaymantami, lanchata apakkunaka mana manchata wiñachi tukunkuna, ni papa yura washakunapi mana tiyarinchu lanchata wiñachinkapak shinallatak wañushpami tukurin. .Chay sinchi muyutaka mana candadota paskay tukunkakunachu. Lanchachaka waranka warankami wiñarin shinaka ima pachapish shuktak shina muyukunata wiñachishpaka unkuchita ushankakunami. Sinchi muyukuna kachunka fungicidakunata churana. Ashka watakuna washami sinchi muyukunaka mana sinchi kay tukuns. Muyu kunanlla yaykushka kashpaka mana ashka muyukuna tiyanchu chaymantami ashalla tarpushkakuna tiyan. Chaymantaka, tukuy unkuyta apamukkunami chayashpa wañunllakuna.2. Muyutaka tarpukkuna purakunarakmi fukuchita kallarinkuna chaypimi punta yurakuna unkushka rikurin.Chay muyukuna alli fukushka kakpika ashtawanmi tarpuyta kallarinkuna. Ashalla espora kakpipish kunanka jawalla unkuchi tukunllami ashka yurakunapika. Shinashpaka rikuri kallarinmi tarpushka ukupi ashka unkushka yurakuna.Chay yurakunamanta manchakunaka kunanka wiñachi esporakunata wiñachi tukunmi shuktak alli yurakunata unkuchinkapak.Tarpushkakuna mirarishka, shinaka kunanka tukuy lanchata apakkunami unkuchi tukunkuna chay yuritakunata. Chaymanta, sinchi muyuka ña mana sinchi kanchu.Mana sinchi yurakuna kay tukun lancha ashtawan mirarishkamanta. Papa yuraka mana cambiarin, ashtawan panakakuna mirarishkallami.Muyukuna ña sinchi kanata sakishpaka kipataka tukuy tukunmi kutinllatak sinchi muyu mana kashpaka mana sinchi muyu. Inmunes ninkuna unkuymanta jarkariklla muyukunata ¿Imamanta mana unkurin jarkariklla sinchi muyu? Yurakunapak jarkarinaka shuk candado shinami lanchata apakkunaka lleve shinami kan shinapish mana jawalla pashakanchu. Lanchata apakkuna mana alli llaveta charishpaka mana paskanchu, chaymantami mana unkun. ¿Kutin imamanta shuk pachakuna washka jawalla unkunkuna? Chayta alli jamutankapakkarikushun katik killkashkata.shinaka sinchimi kan. Ashka lanchata apakkunami mana unkuchi tukunkuna mana alli llavekunata charishkamanta.Chay unkuchi tukuypak lanchata apakkunaka jawalla yurakunapi urmashpa manchakunata shinallatak wiñachikkunata churay tukunllami. Shuk mancha wiñarikpika tukuy lanchata apakkunami alli sinchi muyukunata unkuchi tukunkuna.shinallatak ña ama sinchiyashpa katinchu. Ashka lanchata apakkunami unkuchi tukunkuna alli sinchi muyukunata.Shuk lanchata apakka alli llaveta chari tukun candadota paskankapak, ashalla yurakuna tiyashkamantaka, pampaman urmashpa wañunllakunami.Lanchata apakkunaka mana wiñachi tukunchu manchakuna ni lancha wiñachikkunatapish. .Lanchata apakkunaka manchakunata shinallatak wiñachikkuna ruray tukunmi.Tukuchinkapak ruraykuna Yachanata ashallayachishka Kay rimashkakunamantami yachana yuyaykunata sinchiyachinkapak ashallayachishun:Imatak kan sinchi muyu shinallatak mana sinchi muyu.Imashinatak shuktakyachin sinchi muyumanta mana sinchi muyumantapish. Imalla allikuna tiyashka sinchi muyukunata charikpika lanchamanta jarkankapak.(Paktashpa) Imatak sinchi jarkariklla muyu, imamantatak mana sinchi muyu tukuy tukun. Kaypakka kushka rikcha pankakunata rikunallami. Chay pachallatami rikuna kutichishkakunatapish imatami killkashkakakunata rikunkapak kunanlla yachashkawanpish.Kay yachay rikuytaka ruranatakmi.Yachana yuyaykuna paktashkata yachakapakka yachakkunamanmi mañana uraman rikuchishka ruranakunata rurachunkuna: Shuk shuyushkapi ima sinchi shinallatak mana sinchi muyukuna kashkatapish rikuchina, kimsa imashina kakta rikuchishpa shinallatak alli sinchi muyukunaka imashina yanapan lanchamanta jarkarinapak. Lanchamanta rikushpa rikuchina shuk tablapi imashinalla kakta shinallatak llaktapi tiyak muyukunaka mashna alli kashkatapish. Paktashpa. Tantarishkapak ñawpakpi rikuchina imatak kan sinchi muyu shinallatak imamanta mana sinchi tukushpa unkuri tukun, ruranapi rikuchishkata katishpa chaytaka rurana kanchik.Minishtirikpika yachakkunata mashnata yachashkata rikuna tukun chaypakka japitukunmi panka 79 manta tapuykunata. Chay washallatakmi alli kutichishkakunata willana kan chaymanta yachakukkunawan rimanakuna, 1.2.Ay yachanamanta yachakukkuna imatami yuyanta tapuna. Kipataka kay tapuykunata rurana Minishtirinchu yachana sinchi muyumanta shinallatak mana sinchi muyumantapish ¿Imapak?: ¿Ima llakikunatatak tarirkankichik? ¿Pachakunaka paktachu karka? ¿Rurankapakka pachakuna paktachu karka? Tantanakuyta manarak kallarishpa yanapak allichirinapakKay yachaykunaman yaykunapakka yachana kamu 1, 2 shinallatak 3 tami yachana kanchik.Pachakuna Kay tantanakuypakka chusku pachakunata minishtinchik. Kallari rimay Lancha japita fungicidakunata churashpa jarkana ashka allimi, chaypakka minishtirinmi yachana ima chakrushka fungicidakuna kakta, randinapak fungicidapak shutita, imashina jampin shinallatak imashina churanapish. Shinallatak riksinami randina wasikunapi tiyak jampikunata. Yachanapishmi mashna alli fungicida kakta, unkuymanta jarkanata shinallatak datos del campotapish, kaykunata: mashna lanchakuna rikurikta tarpushka ukupi shinallatak papa tarpushka kanllamanpish, lancha japina pachakunata, sinchi u mana sinchi muyu kakta, jampishkamanta pacha imashina wiñakta. Kay yuyaykunami ashka alli kan ima fungicidata churanata yuyarinkapak shinallatakmi mashna kutin jampinamanta rimashun kay yachanapika.Paktaykuna Kay yachanata tukuchishka kipaka yachakukkunaka kaykunatami ruray ushankakuna: Shuk rikuchita rikuchishpa, nina ima fungicida kashkata.Rikuna imashina chakrushka jampikunata, rantina shuti, imashina jampin (karkak mana kashpaka kampik fungicida kashkata) shinallatak imashina churanata ishki fungicidakunawan, ima kashkata rikuchishpa. Kay yachanapi kushka fungicidakunamanta rikuchik pankapi rikuna imashina chakrushka kakta, rantina shutikuna, imashina unkuyta jampik kashkata, shinallatak jampinapak imashina chakruna kashkata. Tantanakushka ñawpakpi rikuchina ima yuyaykunata charinata manarak jampikunata churashpa: mashna alli kakta, lanchata jarkakta shinallatak datos del campomantapish (mashna lancha tarpushka 1.3.ukuman shinallatak kanllaman tiyan, ima pachakunapi mirarin, ima papakunatak jarkarin, imashina wiñan jampishkamanta pacha). Ima jampiwan jampinata yuyarina shinallatak mashna kutin churanamantapish, kay yachanapi rikuchishka yuyaykunawan rikuchi tukunkallami.5.. Allimanta kay killkata rikuna kan Yachayruray 1, 2 shinallatak 3 pi nikuk minishtirishkakunata mashkana. Rikcha killkakunata japina kay pankakunamanta 90, 93, 95, 98, 106, 107 shinallatak 137manta 142kama (Tantachishka 2) (chay rikcha pankakunaka tukuypak kana kan). Paktashpaka. Minishtirikpika yachakukkuna yachashkata rikuchi tukunmi chaypakka allichinami shuktaka yachayta kallarinapak shinallatak shuktaktaka tukuchinkapak (panka 113manta, 114kama shinallatak Tantachishka 1). Wayrakun pambata mashkashpa tantanakuna Yachayruray 1ta rurankapak mana kashpaka wichkashka ukupimi rurana kanchik 2 -3 Yachayruray rikuchikushkata.1. 2. Kay ruraytaka ashka wayra tiyanpimi rurana wasikunamanta karuyachishpa. Mana tiyachinakuna jawapi ruranachu ashatawankarin pampapimi rurana. Yachakukkunawan ima yuyaywan kay rurayta rurakushkamanta willana. Kimsa u pishka uchilla tantanakuykunapi fungicidakunata kuna (Mirachishka yuyay 1). Chay uchilla tantanakushkakunapika shukllapish killkana ñawirinata yachakmi tiyana kan. Chay tantanakuykunamanka panka 90 manta llukchishka rikcha pankakunata kuna kan.1.3.Yachana Kamu 4Chay jampi wakichinataka chushaklla kakpipash mana kipataka imapak minishtirinchu, mayllakpipish chay fungicidaka sakirinkallami chaymanta kikinta wakllichi tukunmi, ñatak wakichishpa kutin imapak japikunkichikman. Chay jampi apamuk fundaka alli wichkashka kana. Ama jicharichunka shuk achiklla plástico fundapimi churana. Guantesta churana. Mana imata mikuna fungicidakunawan yachay pachakunapi Mana wawakuna kuchuyayta sakikunachu shinallatak wasi ukupi kausak wiwakunatapish. Rurayta tukuchishpaka makikunata allita mayllana. Hongokunamanta wacharishka unkuykunata jampik wañuchik jayak jampikunami kan.Rantina shuti Katunapak churashka shuti.Wañuchik jayak jampipak shutimi kan.Shuktak shukta shutiwan jampikunapish tiyanmi, chay jampika chayllatakmi kan. Mayjan jampika ishki shina jampikunawan chakrushkami. Kayshukkunaka ishki mana kashpaka ashtawan jampikunawan chakrushkakunami tiyan. Yakumanta ishki shina rikuchita rikuchina: razuta shinallatak yaku (razu -yaku). Yachakukkunaman tapuna razuwan yakuwan chikan kashkata.Chaykunatami chimpapurashpa rikuchina chay fungicidakunapish kuta kay tukun mana kashpaka yaku shina chuyapish kay tukunllami. Yachakukkunaman mañana shuktak chay shina rikuchikunata churachun (Mirachishka yuyay 2). Tantanakushpa rimanakuna imatak kan fungicidakunata chakruy. Yachakukunawan rimanakuna ima allikuna tiyan shinallatak imalla mana allikuna tiyan chay ishki shina chakuykunamanta. Yachakukkunaman kushka fungicidakuna imalla jampikunawan chakrushka kashkata rikushpa panka 90pi killkachunkuna mañan kanchik. Yachakukkunaman rikcha pankakunata kuna (panka 95) fungicidakuna chakuy ima nisha nishkata shinallatak ima kashkatapish achikyachunkuna.Yachana Kamu 4Yachashun imatak jampikunata chakrunaImashinami fungicidakuna tiyan: Shukka kankunami jarkaka mana kashpaka jampik (jarkak -jampi).Chay kuta fungicidakunaka ashka llakichikmi kan. Chakrukushpaka ashnayta wayrapi aysay tukunchikmi shinallatak ama tanquepi sakirichunka ashkatamami kuyuchina kan. Shinapish, kuta fungicidakunatami ashalla chanipi ranti tukunchik yaku fungicidakunaka ashawan kullki chanimi kan.  Kuta fungicida Yaku fungicida Kuska 4 Yachashun fungicidakuna ima rurakta.Kayta rurankapakka kimsa mashikunapak yanapayta mañana: Mama Esperanzaka ishki wawakunatami charin, Lucho shinallatak José wawakunata. Luchoka lluki chaki waktarishkami shinallatak Josétaka ujumi japishka. Esperanzaka yuyarinmi Luchotaka wirawan kampinata shinallatak Josétaka pastillakunawan jampinata. Wirata churakukpika Luchoka ninmi shuyay rikusha, mamaka ninmi kayka aycha jawallatami jampin. Luchoman pastillata kushpaka ninmi kayka aycha ukutami jampin. 2. Tantanakushpa rimanakuna fungicida ima rurakta yuyayta llukchina. 3. Rikuchina imami karka jarkak fungicida shinallatak jampik fungicidapish cartulinawan rurashka papa pankapi, chay panka celofánwan, chay yurak pilluchishka uchilla papelkunawan mana kashpaka espuma sintéticawan, shinallatak algodónwan, kayta rurankapak kashna ruray: Chay puka panka celofánwanka pilluchina cartulinawan rurashka pankata kayka jarkak fungicidawanmi nishpa. Uchilla pilluchishka pankakunawan mana kashpaka espumawanka rikcuhinami lanchata apamuk kashkata. Lanchata apak chay fungicida churashkapi chayashpaka wañunllami nishpa rikuchina. Chay panka ukupi algodónta churashpaka ninami lanchata wiñachikmi papa yuraman yaykushka. Chay jawapika puka panka celofánta pilluchina chaymantaka ninami kay jarkak fungicidaka mana imata ruran yurapi ña lancha wiñakukpika. Jarkak fungicidakunataka kay pachakunapimi churana nishpa ashka yuyaypi kana.Mana ashka tamyakuy punllakunapi. Manarak manchakuna yurapi rikurikpita. 9. Kuska 5Yachakukkunaman kuna fungicidakunata imashina apanata rikuchik rikcha pankakunata (Tantachishka 2), chay pankawanka jampikunata rantinaman rinkapakpish yanapankami nishpa willana kanchik (Mirachishka yuyay 3). Yachakkunata amañachina fungicidakunata imashina apana fankata juntachinata yachachunkuna shinatallatak chay pachallatak mañana chay pankata juntachichunkuna katunapak shuti, chakrushka jampi, imata rurak shinallatak mashnata churana lancha unkuymanta jarkankapak.1.Killkana ñawirinata mana yachak mashikuna fungicidakunata rantishun nikpika ñawirinata killkanata yachak mashiwan ushashpaka rina kan fungicida jampikunata rantinapi yanapachun.Paktaykuna Kay yachayta tukuchishka washaka yachakukka tantanakushkapak ñawpakpi rikuchi tukunkami kay yuyaykunata charinata manarak fungicidakunata churashpa: mashna alli kashkata, imashina lancha ama papakunata japichun jarkanata shinallatak datos de campo japinamantapish (tarpushkapak muyuntikpi shinallatak tarpushka ukupipish mashna lancha tiyakta, punllakuna imashina kakta, sinchi -mana sinchi papakuna, yurakuna imashina wiñakukta shinallatak fungicida churashka punllamanta mashna punllakuna yallishkatapish).Jatun pankakuna shinallatak jatun killkana kaspi marcadorkuna.Imamanta kay rurayta rurakushkamanta yachakukkunaman willana kanchik. Jatun pankata charina chaypika killkashpa katinalla imallata rimashkakunata. Kay rimanakuykunata rukuchishka washaka Panka 106, 107 pi rikuchishka shina shuk cuadrota rurashpami tukuchina kanchik. Yachakukkunawan shuyuna imashina lancha wiñarik kashkata (Yachana 2 panka 53 rikuna). Kay shuyushkataka minishtishunmi shamuk rimaykunapakpish.Yuyarishun fungicidakuna mashna alli kashkata Yachayruray 1, Kuska 4-ta yuyarina. Shuyushpa ashata rimanakuna jarkak shinallatak jampik fungicidakuna yurapi churashka washa ima tukukta. Kaytaka imanakuytaka chimpapurashpa rikuna lancha imashina wiñarikta:Jarkak fungicidakunaka kutakunata shina pankakunapi tiyarishkakunallatami wañuchin mana manchakunataka jarkanchu. Jampik fungicidakunaka yuraman yaykurishka hongokunata wañuchinmi. Wiñarikuk manachakunata wañuchinmi.2. Tantanakushpa rimanakuna mayjan fungicidatak ashtawan alli kanka nishpa. Manarak fungicidakunata churashpa yuyarinami kanchik mayjan fungicidatak pakta kashkata nishpa willana kanchik.Riksishun imamantatak yurakunata lancha ama japichun jarkana allí kakta Rikuchina shuk metal jawapi óxido ima tukukta shinallatak shuyuna pintura imata ruraktapish. Yachakukkunawan rimanakuna imapak shuk metalta pintanaka minishtirin ama oxidachun. Wawakunatapish uchillarak kashpa vacunanchikmi paykunata ama unkuy japichun nina. Chay shina rikuchikunata churachun yachakukkunamanpish mañana. Imashina chay lancha unkuy wiñarik kashkata rikuchihspa allita yuyachina chay jarkak fungicidakunami pankakunaman ama lancha chayachun jarkak kashkata.Tantanakushpa rimanakakuna imamantatak yurakunata ama lancha japichun jarkana alli kashkamanta.Riksichun mayjanlla dato de campota charina kanchik manarak fungicidakunata churashpa.Kay datoskunatami yachakukkunawan rimanakuna kan: » »Tarpushkapak muyuntikpi shinallatak tarpushka ukupipish mashna lancha tiyakta Punllakuna imashina kakta Sinchi -mana sinchi muyukuna Yurakuna imashina wiñakun Fungicida churashka punllamanta mashna punllakuna yallishkata.Tarpushkapak muyuntikpi shinallatak tarpushkapak ukupipish mashna lancha tiyakta Shuk jatun panakta chawpina. Chay chawpishka pankakunapika cuadrokunata shuyuna papa tarpushkakunata rikuchishpa shinallatak chay cuadrokunapak chawpipika shuk llaktatapish shuyuna. Ishki shuyushka llaktakunataka shutikunata churachun nishpa yachakukkunaman mañana. Allpapi llankak (Juan) mashi chay ishki llaktakunapi papa tarpushkata charin nishpa rikuchina. Chay ishki tarpushkakunaka kaykunami nishpa shuyuta rikuchina. Mashi Juanpak kuchilla tarpushkakunapi shinallatak Juanpak tarpushkapipish lancha ña japikushka manchakunata shuyuna. Shuk niki llaktapika ashalla manchakunata shuyuna, ishki niki llaktapimi ashka manchakunata shuyuna. Yachakukunawan rikuna Juanka mayjan llaktapitak ashtawan fungicidakunata churana kan. Lancha wiñarikukta rikuchishpa allita yuyachina wayrapi esporakuna tiyakpi fungicidata churana kashkata. Kay lanchaka tiyay tukunmi kuchulla tarpushkakunapi, mayjan yurakunapi, sacha yurakunapi shinallatak tarpushka ukumantatak unkushka yurakunapi. b. Punllakuna imashina kakta (tamya shinallatak asha junuklla chirilla punllakuna) Lancha wiñarikukta rikushpami yuyarina imatami tamya shinallatak asha chirilla junuklla pacha kashpapish rurak kashkata. Shuk unkushka yuramanta kayshuk alli yuraman apan tamyawan salarishpa Jampishka fungicidata panka jawamanta mayllan. Chirilla kunuklla kay pachakunaka yanapanmi manchakuna wiñarichun, Ashtawan junuklla kapika ashtawan utka manchakunaka wiñarin. Yachakukkunawan rimanakuna tamyakuy pachakunapi shinallatak kunuklla kay pachakunapimi ashtawan fungicidata churanamanta, chakishka shinallatak chiri punllakunata yalli.Jatun pankapi shuyuna ishki papa pankakunata, shukpika sinchi muyupak pankata shuktakpikka mana sinchi muyupak pankata. Yachakukkunawan tikra rikuna kimsa yuyaykunata imamantatak sinchi muyukuna shinallatak mana sinchikuna kaktapish (Yachana 3, Panka 66 rikuy). Tantanakushpa rimanakuna imamantak mana sinchi muyuka ashka fungicidata minishtin sinchi muyutapish yalli. Yuyarina ashalla fungicidata churashpaka fukuchinkapak mana ashka kullki minishtirin shinallatak mana pachamamatapish llakichinchik. Fungicidakunawan lanchata jarkanapakka kaykunatami yachana: Mashna alli fungicida kakta. Imamanta lanchamanta jarkarinamanta. Datos del campotapish yachanam.1. Fungicida mashna alli kan 1.Jampik fungicidakunami ashawan alli jampikuna kan chayshuk jarkak fungicidakunatapish yalli shinapish rantinapakka yalli chanimi shinallatak mana alli fungicidapish kay tukun.Chay lanchaka utkashpa llakichik shuk unkuymi: japikpika shuk kanchisripi ñami tarpushkata chinkachinllami. Chaymanta lanchata wiñachi tukuypak pachakuna kakpika utkashpami fungicidakunata churana kanchik manarak manchakuna yurakunapi rikurikpi. Yachana minishtirinmi mashna punllakunatak fungicida churashkamantaka yallishka shinallatak chay punllakunaka imashina karka, tamyaka fungicidata mayllanllami. Asha punllakuna ñawpaman fungicidata churashka kakpi kipa punllakuna usya kashka kakpika pankakunapi fungicida tiyakunkarakmi. Shinaka mana jampina minishtirinchu.Ñawpalla punllakuna fungicidata churashka kakpi chay kipaka tamya urmashpalla katishka kakpika pankakunata mayllashkankami, chaypika minishtirinmi kutinwan jampina. Tantanakushkapak ñawpakpi rikuchina imamantatak manarak jampikunata churashpa fungicidakunatarak riksina: alli kakta, lanchata jarkakta, datos del campo nishkamanta (tarpuska kanllaman shinallatak ukumanpish mashna lanchakuna tiyan, ima pachakuna mirarin, ima muyuwan tarpushka kakta, fungicidata churashkamanta wichimanka imashina wiñashpa katin).1.3.Ima fungicidata churana shinalaltak mashna kutin, imalla pachakunapi, kay yachana kamupi rikuchikuk yuyaykunawan).Kay yachay rikuytaka rurasha nishpalla ruray. Yachashkata ashtawan rikusha nishpami kay tapuykunata kutichichun mañana kanchik, kay tapuykunaka Panka 113, 114 pi killkashka tiyakun, imashina ruranataka Tantachishka 1pimi willakun. Chay tapuykunata kutichishka pachallatakmi alli kutichishkata rikunachina kan shinallatak yachakukkunawan parlanakuyta rurana.Imata yuyashkata tapuna kay yachaymanta. Chay kipaka kay tapuykunatami churay tukunchik: ¿Rurashkakuna achikchu karka? ¿Imashinatak sinchiyachi tukurin? ¿Ima llakikunatak tiyarka? ¿Yachaykunata chaskinapakka pachakunaka paktachu karka? Pacha Shuk pachata tantanakuna.Kallari rimaykuna Kay yachana kamuka kayshuk yachanakunamanta rikukpika ashallami kan, shinallatak ruranami papa tapushkakunata rikunaman rishka pachakunapi. Papa unkuyka utka mirariklla unkuymi, chaymantami kanchisripi shuk kutinllapish rikushka kana kan. Chay rikuykunaka yanapanmi lancha unkuykuna japikushkata yachankapak, chayta rikushpa imashina jarkana mana kashpaka mishanata yuyarinapak. Kay yachanaka rikushkami kay tukun análisis del agro-ecosistemawan metodología del Escuela de Campo de Agricultoresta rurashkawan (ECA).Paktaykuna Yachakukkunaka kay yachaykunata tukuchiska kipaka ushankami: Tantanakushkapak ñawpakpi rikuchinata lancha unkuyta jarkankapak imapak katirayashpa tarpushkata rikurana alli kashkata. Imalla ruranakunamantapish lancha japichun jarkankapak tarpushkata rikushka yuyaykunawan, ima jampi churana, imashina jarkana, ukuman-kanllamanpish mashna yurakuna unkushkata, ima pachakuna, jarkarik sinchi papakuna shinallatak jampishkamanta pacha wichimanka imashina tiyakukta rikuchinata ushankami.1.Allimanta kay killkata rikuna kan. Yachayruray 1 shinallatak 2 pi rikuchikuk minishtirikunata charina. Rikcha pankakuna 123manta, 127 kama charina (Tukuy pankakunami yachakukkunapak tiyana kan).1. 2. Shuk allpapi tarpukka papa tarpushkatami charin Loma Grande nishka llaktapi. Chay mashikuna kawsana llaktaka 2.900 metros mama kucha jawamanmi sakirin Kulla killa 2 punllapimi tarpushka karka. INIAP-Gabriela nishka muyutami tarpurka, chayka lanchata jawalla japina muyumi. Mashna yupay shinallatak yuyaykunata rikuchik tablapi rikukpika, sinchi muyuta rikuchikpika kimsa casilleropimi yaykun. Chay mashna yupayka tukuy tarpunka kamami sakirin. Papata tarpukka febrero pishka punllapi tarpushkata alli mana alli kaktapish rikurka.Chay pachakunaka ashka tamya pachakunami karka. Yuyaykunata shinallatak mashna yupaykuna rikuna tablapi rikukpi, casillero 2 pimi kana kan 'ima pachakunata rikukpika'. Tarpushkaka alli wiñakuy pachapimi karka. Chaymantami, 2 niki yupayka maypimi kana casilleropimi yaykun. Tarpushka kanllaman shinallatak ukumanpish ashka lanchami tiyarka, Chaymanta, 3 niki yupay chaypi kana kan. Panchi killa 5 punllakamaka mana ima fungicidata churanachu. Chaymantami, \"punllakuna ima pachamantami fungicida churashkamanta\" casillataka yurakpimi sakina.Tarpukkunaka ñawpaman mashna yupaykunata mirachishpa chunkamanmi chayankuna, \"Ima ruranata rikuchik\", chaypika jampik fungicidatami churana nikurka. Chay pachallatak yuyarirka lanchata jarkana ashka alli kashkata. Chay datoskunawanmi yuyarirka nishkakunata chaskinata shinallatak jampik fungicida churanata. Kanchis punllakuna washa (panchi killa 12 punlla) tarpukka kutinllatakmi imashina tarpushka tiyakukta rikurka. Chay pachakunaka ashka chakishkami karka. Yuyaykunata rikuchik shinallatak mashna yupaykuna rikuna tablapika 1 niki yupaymi chaypakka. Tarpuyka wiñakuy pachapimi katirkalla. Chaymantami, 2 yupaypak kan. Tarpushka kanllaman shinallatak ukumanpish mana ashka lancha tiyanchu. Chaymantami, chaypakka 2 yupay kan. 7 punllakunami fungicidata churashkamanta pachaka. Chaymantami , shuk niki chayñiman rina kan. Tarpukka ñawpakunamanta tukuy tantachishpaka 9 tami japirka. 'Ima rurana rikuchik\" tablapi rikuchikpika, jarkak fungicidata churana nishka karka. Shinapish, anki uyaripi uyarkami sinchi tamyata chay llaktapak chaymantami mana llakikunata charinkapakka shuk jampik fungicidata churanatami yuyarirka. Tantanakuyta manarak kallarishpa yanapak allichirinapak Kallarichi rimay Kay yachashkata rikunkapak nishkataka prueba de caja, prueba de campo mana kashpaka prueba de chakra shutiwanpish riksishkami kan. Kayka shuk yachayñanmi kan Escuelas de Campo de Agricultores (ECAs)pika kaywanmi llankankuna. Kay ECA nishkata kallarishpaka kay tapuykunaka yachakkuna yachashkata rikunapakmi kan, ima illakpika chaytapish yachaypak allichinkapak. ECA ta tukuchishka kipa tantanakuykunapi yachashkakunatami kay yachayñanwanka rikuchinka. Kay yachayñan yachashkatak rikunkapak killkawanka kallarinkapak shinallatak Yachana kamuta tukuchishpapish minishtirishka kana kan chashnallami yachakukkuna mashna yachakushka mana yachakushkatapish riksishun.Ñawpak allichirina Shuk mana kashpaka iski punlla ñawpaman. Abreviaturas: cc = centímetro cúbico, g = gramo, kg = kilogramo, ha = hectárea Equivalencias: 1 litro = 1.000 cc, 1 kg = 1.000 g, 1 ha = 10.000 m 2","tokenCount":"6524"} \ No newline at end of file diff --git a/data/part_6/1124999168.json b/data/part_6/1124999168.json new file mode 100644 index 0000000000000000000000000000000000000000..a94e4d31688fc550ccb1c6c286108055c12da12c --- /dev/null +++ b/data/part_6/1124999168.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"c0587aac35f7eafe17a017aaef560c5d","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/1c846b59-3c78-4fbc-972b-d7a2cc49c5d8/retrieve","id":"-270233058"},"keywords":[],"sieverID":"dd091471-6f67-42a4-b204-0e87cb72ec7a","pagecount":"13","content":"desarrolló un taller para trabajar con familias rurales en el fortalecimiento y comprensión de conceptos sobre cambio climático, vulnerabilidad y adaptación al cambio climático. Al mismo tiempo se pretendía sensibilizar a los agricultores para asumir un rol más activo frente al cambio climático en su territorio.Durante la sesión se preparó un plan de trabajo de acuerdo a los avances y las lecciones aprendidas, en el que los agricultores definieron de manera participativa los temas y actividades para para el manejo y la implementación de prácticas agricultura climáticamente inteligente (ACI) que demanden los sistemas productivos.Con este taller continuo la preparación de los hogares rurales para usar información climática que les conduzca a implementar mejores prácticas de adaptación en sus sistemas de producción.América Central y Nicaragua por su geografía son zonas de alta vulnerabilidad, caracterizadas por ser parte de los territorios más susceptibles del mundo a sufrir los efectos adversos del cambio climático global y de la variabilidad climática, debido al nivel de exposición de vastas zonas a las diferentes amenazas e impactos de los eventos vinculados al cambio global y a la variabilidad, unido al predominio de ecosistemas frágiles, altos índices de pobreza y un uso inadecuado de la tierra (Milán 2009).La agricultura familiar, representa sin lugar a duda, para muchas familias de la región centroamericana una de las principales actividades económica generadora de ingreso y que dan soporte a la seguridad alimentaria nutricional, sin embargo, los sistemas de producción han sido afectados de forma recurrentes por los efectos del cambio climático y la variabilidad climática.La situación, en vez de mejorar, empeora, ya que este sector se caracteriza por un acceso limitado a bienes de capital, infraestructura, asistencia técnica, recursos humanos e institucionales, información, recursos tecnológicos y financieros, que explican su baja capacidad de adaptación y respuesta (CEPAL et al. 2015).A diferencia de otras actividades económicas, la agricultura se caracteriza porque la producción es una variable aleatoria, influenciada principalmente por el clima y sus efectos, además de que la demanda y la oferta son inelásticas, lo cual ocasiona que los precios e ingresos sean altamente volátiles (CEPAL et al. 2015).En esta sesión se pretende introducir y reflexionar con familias integrantes de ECAs multirubro y multitemáticas, elementos conceptuales relevantes para trabajar sobre el tema de cambio climático, vulnerabilidad y adaptación al cambio climático. También se espera reconstruir el proceso de aprendizaje usando el enfoque de ECAs implementados por el CATIE a través de su programa MAP entre 2013-2016 y preparar un plan de trabajo para el segundo semestre del 2017 de forma participativa que rescate los avances, las lecciones aprendidas y continuar con el fortalecimiento de hogares rurales de forma que estén mejor preparados para usar información climática que les conduzca a implementar mejores prácticas de adaptación en su sistemas de producción, asentados en el TeSAC el Tuma -La Dalia.Las personas participantes:• Reflexionan sobre los resultados alcanzados a través de las ECAs desarrolladas con proyecto MAPNoruega y los retos inmediatos y futuros para mejorar su capacidad de adaptación. • Fortalecen sus conocimientos respectos a los conceptos de vulnerabilidad y cambio climático.• Discuten y definen de forma consensuada un plan de trabajo a desarrollarse durante el periodo de julio-diciembre 2017.Productos tangibles al finalizar la sesión.• Las personas participantes, tienen un entendimiento práctico sobre los conceptos de vulnerabilidad y cambio climático. • Un plan de trabajo consensuado, que delimita las acciones a desarrollan con las familias entre julio y diciembre del 2017, con una mirada prospectiva hacia el ciclo agrícola 2017-2018.Aspectos técnicos que requiere la persona facilitadora para hacer estas actividades con familias ECAs.• Recorrido de campo por las fincas de las familias que participaron o participan de las ECAs, para conocer los avances del plan de patio y finca realizado en años anteriores y las acciones prioritarias de cara a los próximos 6 meses del presente año (julio-diciembre 2017). • Tener documentado (resumen), de las acciones que las familias han priorizado para los próximos 6 meses, según sistemas productivos priorizados en sus fincas (café, pasturas, granos básico y producción de patio). • Considerar los aspectos de logística, en este caso, definir el sitio más adecuado para hacer la sesión (considerar un punto céntrico en la comunidad), hacer la convocatoria al menos con una semana de anticipación para garantizar la participación de las familias (dos personas por familia, un hombre y una mujer), garantizar los materiales y la alimentación requerida para participantesHabilidades metodológicas que requiere la persona facilitadora:-Conocimiento y manejo de herramientas de capacitación participativa (uso apropiado de preguntas para explorar, promover el análisis, el razonamiento agroecológico y climático. -Dominio del contexto en función de aspectos clave relacionado con clima Tiempo aproximado para la sesión (tres horas y media).-Bienvenida, presentación, objetivos y metodología de la sesión (30 min).-Hilo conductor de proceso ECA MM y su evolución hacia una ECA+++ (30 min) -Plan de trabajo conjunto con la comunidad junio-diciembre 2017 (60 min).-Homologación de lenguaje relacionado con el tema de CC y vulnerabilidad climática. (60 min). -Acuerdos y evaluación de la sesión (15 min)-Papelones (20 unidades), marcadores de 4 colores (una caja), cinta adhesiva (dos rollos) -Dos cuerdas -Un porta rotafolios -Tarjetas de diferentes colores (30 unidades)Desarrollo del evento Paso 1. Bienvenida, presentación, objetivos y metodología de la sesión.La sesión inicia con una breve introducción, en este caso estará a cargo del personal facilitador de NITLAPAN, acá se destacan temas como la relación de CATIE-NITLAPAN-CCAFS y sus contendidos de trabajo en el TeSAC, hacer referencia particularmente al trabajo previsto con las familias a través de las ECAs y los impactos esperados de estas acciones.Después de la breve introducción se hace la presentación de participantes, a través de la \"Dinámica de Telaraña\".Se pide al grupo que formen un circulo. Ideal si se hace en un espacio abierto fuera del salón. La persona que facilita se ubica en el círculo, sosteniendo una madeja de hilo o cuerda. Se explica la dinámica, la cual consiste en lanzar la madeja a cualquier persona escogida al azar.La persona que recibe la madeja, debe decir en voz fuerte su nombre, lugar de procedencia y si posee algún cargo en la comunidad, o si pertenece a alguna organización asociativa. Sin soltar la cuerda este lanza nuevamente la madeja, a otro asistente al azar, el cual procederá de la misma forma hasta haber incluido con todos/as y formado la red.La persona que facilita explica entonces que la RED construida, es un ejemplo de lo que se quiere hacer con la metodología \"Construir conocimientos con la participación de todos/as y una red de trabajo en torno a la adaptación al cambio climático\".Luego se presentan los objetivos, los contenidos y la metodología del taller, explicando brevemente cada uno de estos tópicos, y se pregunta al grupo, si desean agregar o quitar algún aspecto en la agenda propuesta para la sesión de trabajo del día.Paso 2: Hilo conductor de proceso ECA MM y su evolución hacia una ECA++.La persona que facilita pide a los y las participantes que en forma de lluvia de ideas, recuerden que fue lo que paso con el proceso de aprendizaje realizado a través de la ECAs que desarrollaron entre 2013-2016 con el CATIE y la UCA o La COMULACS (según sea el caso para NicaCentral), para este ejercicio se sugiere usar las siguientes preguntas:-¿En qué periodo se desarrolló la ECA con la UCA o la COMULACS? -¿Qué actividades se hicieron en la ECA? -¿Cuáles fueron los temas de capacitación? -¿Cuáles fueron los temas de asistencia técnica? -¿Cómo podemos valorar el cumplimiento de las acciones que de definieron para el patio y la finca?-¿Que continuamos haciendo después de finalizadas las ECAs con la (UCA o la COMULACS) en nuestras fincas y patios?Las respuestas se van anotando en tarjetas o bien en un papelografo. Al final, la persona que facilita presenta a través de carteles previamente elaborado un resumen del proceso desarrollados en las ECAs MM. Paso 3. Homologación de lenguaje relacionado con el tema de CC y vulnerabilidad climática.Con frecuencia se reconoce que el tema de cambio climático aun es un tema muy académico y que un mejor entendimiento por parte de las familias rurales es un paso importante hacia su sensibilización como actor y receptor activo de este hecho en el mundo que habitamos. En esta sesión de trabajo se pretende mejorar el conocimiento y la comprensión de los conceptos de variabilidad, CC y vulnerabilidad climática, para ello se invita a los participantes a que nos ayuden a desarrollar el siguiente el ejercicio \"Hablemos el mismo lenguaje\" 1 a. Explicar a los participantes, en un lenguaje coloquial.La persona facilitadora del taller, explicará cada uno de los conceptos presentados en esta sesión (ver material de consulta en anexo 1), incluyendo ejemplos de la vida cotidiana, que permitan una asimilación rápida por parte de los asistentes. Las siguientes preguntas guías pueden ser utilizadas para conducir la reflexión.b. Construir con los participantes las definiciones.1 Adaptado de Ortega, L.A.; Paz, L.P. 2014. Manual para la formulación de planes prediales de adaptación a la variabilidad climática.Página 7 de 13De los conceptos básicos en su lenguaje: la persona facilitadora invita al grupo a construir sus conceptos a partir de la exposición realizada por la persona facilitadora.Se forman grupos de 4-5 personas, cada grupo trabajará un concepto; se les entrega tarjetas para que puedan ir definiendo los conceptos o la diferencia entre concepto según corresponda, a partir del entendimiento local y con base a un ejemplo. Los grupos se pueden organizar se acuerdo a los siguientes conceptos:Grupo 1: clima y tiempo Grupo 2: variabilidad climática y cambio climático Grupo 3: efecto invernadero y calentamiento global Grupo 4: fenómenos extremos Grupo 5: adaptación Grupo 6: concepto fenómenos del niño y la niña c. Socialización de los resultados por grupo de trabajo:Cada grupo elige a una persona que los representa para exponer sus definiciones, las cuales son pegadas de forma visibles para todos/as. Una vez finalizada la socialización de los grupos, la persona facilitadora debe hacer un resumen de los resultados.Paso 4. Plan de trabajo conjunto con la comunidad junio-diciembre 2017.Con el objetivo de concertar un plan de trabajo y acordar compromisos con las familias; la persona facilitadora elabora una propuesta de plan de actividades para el periodo (en este caso es de julio -diciembre 2017). Esta propuesta se elabora una vez que el facilitador haya visitado todas las familias participantes de la ECA, ya que el contenido del mismo se fundamenta en las actividades prioritarias que las familias mencionen en la visita de asistencia técnica realizada por el facilitador previo a esta actividad.Las sesiones de escuela de campo, los contenidos de asistencia técnica u otras acciones que queden propuestas en el plan de trabajo, además de abordar la herramienta PICSA, dará repuestas a las demandas de conocimientos y habilidades técnicas que hayan sido identificadas y/o demandadas por las personas involucradas en la implementación de la ECAS+++ (familias productoras, personas facilitadoras y especialistas), para el manejo y la implementación de prácticas ACI que demanden los sistemas productivos priorizados (café, cacao, granos básicos, ganadería y el patio).En este caso se presentará una propuesta consolidada que retoma contenidos propuestos por las 30 familias integrantes de los dos grupos ECAS+++ representantes de 2 de las 7 comunidades que conforman el TeSAC \"El Tuma -La Dalia\". En este ejercicio las personas participantes pueden avalar, modificar y/o añadir actividades a fin de que se puede tener un plan de trabajo consensuado y de interés para todos/as. El resultado del ejercicio regirá los contenidos a abordar en el resto del periodo del año en curso y será una herramienta de guía en el proceso de fortalecimiento de capacidades en las ECAs+++.Paso 5. Acuerdos y evaluación de la sesión.En un cartel o papelón visible para todo el grupo, se van anotando los acuerdo y próximos pasos a seguir en la escuela de campo.Antes de finalizar la sesión se realiza la evaluación del día, para este ejercicio se utilizará la dinámica, del estado del tiempo (soleado, nublado y tormenta), colocada en un cartel o papelón donde cada participante marcara con una \"x\" cómo valora \"el tiempo\" que se vivió en la sesión. Por ello, el estudio del tiempo está a cargo de la meteorología, mientras que el clima está a cargo de la climatología. Como se puede observar entonces, el tiempo es una característica de un momento o un día, mientras que el clima, hace relación a la colecta de datos diarios durante meses, años o cientos de estos, que permiten hacer análisis estadísticos y definir cómo se comporta una variable (temperatura, precipitación) en un lugar determinado.Así existen fenómenos meteorológicos como las granizadas, el arcoíris, los truenos, los relámpagos y la misma lluvia, mientras que fenómenos climáticos son el fenómeno de El Niño o La Niña.El cambio climático hace referencia a cualquier cambio en el clima, que se manifiestan en cientos o miles de años, ya sea debido a su variabilidad natural o como resultado de la actividad humana. Un ejemplo de ello: Hasta hace 35.000 años la Laguna de la Tota (Boyaca), estaba cubierta de hielo y la temperatura promedio era de aproximadamente 5°C. En la actualidad los hielos han desaparecido, los casquetes más cercanos que aún quedan están a 115 Km de distancia en la Sierra Nevada del Cocuy. El clima actual en la región de La Tota, es frio con una temperatura promedio de 12°C. Se puede apreciar entonces, que ha habido un cambio del clima para esta localidad.Por su parte, la variabilidad del clima, según el Centro Internacional para la Investigación del Fenómeno del Niño (CIFEN), es una medida del rango en que los elementos climáticos, como temperatura o lluvia, varían de un año a otro. Incluso, puede incluir las variaciones en la actividad de condiciones extremas, como el número de aguaceros de un verano a otro. La variabilidad climática es mayor a nivel regional o local que al nivel hemisférico o global.En la siguiente figura, se puede apreciar un ejemplo de variabilidad climática. Durante el periodo 1990 a 2008, los meses de abril, mayo y junio fueron los más lluviosos y diciembreenero los más secos, pero en el año 1991, abril fue casi tres veces más lluvioso que el promedio, mientras que el mes de mayo fue mucho más seco de lo normal. En el caso del año 2002, abril y mayo fueron meses menos lluviosos que el promedio y diciembre un mes mucho más lluvioso.Es común escuchar hoy en día, estos dos términos y asociarlos como si uno fuese consecuencia del otro. A pesar de ello, se debe resaltar que el efecto invernadero es un fenómeno por el cual, gases existentes naturalmente en la atmosfera como el dióxido de carbono, el vapor de agua, el metano, el óxido nitroso y el ozono, retienen la energía que el planeta libera después de haber sido calentado por la radiación solar, lo que permite mantener la temperatura y facilitar el desarrollo de la vida, por lo cual no debe relacionarse necesariamente con algo perjudicial.Este efecto, es aprovechado comúnmente en las zonas rurales, por ejemplo, en el cultivo de tomate. Dicho cultivo, no resiste las heladas, entonces los agricultores los siembran en \"camas\" y los protegen con plástico, estructura conocida como invernadero. Lo que hace el plástico, además de proteger las plántulas de tomates de las lluvias, es aumentar la temperatura y brindarles un ambiente más cálido, que el existente en el exterior, es decir, que el plástico hace las veces de los gases efecto invernadero.El calentamiento global, por su parte, es el incremento en la temperatura promedio de la atmosfera terrestre y de los océanos, generado, principalmente, por las emisiones de gases efecto invernadero (dióxido de carbono, el óxido nitroso, el metano y halocarbonos) derivados de las actividades del ser humano. Así lo confirma el quinto reporte del IPCC, en donde se afirma que la temperatura media superficial global incremento 0,85˚C durante el periodo 1880-2012 y se estima que a corto plazo (2016-2035) incrementara 0,9-1,3˚C y a largo plazo (2081-2100) incrementara 0,9-2,3˚C. Estos valores, determinan, por ejemplo, que el nivel de los mares aumente y ponga en riesgo a las poblaciones que habitan en sus orillas.Entre las actividades humanas que conducen al calentamiento global, se encuentra la emisión de dióxido de carbono por tala de árboles, por utilización del petróleo, el gas o el carbón como combustible, la producción de cal para fabricar cemento. Entre las actividades humanas que generan metano, se encuentran los procesos digestivos del ganado vacuno o equino, al igual que todo aquello que implique la descomposición de la materia orgánica (cultivo de arroz en humedales, vertederos o rellenos sanitarios).Se llama evento extremo a un evento que es raro en un determinado lugar y estación. Los extremos varían de un lugar a otro. Un extremo en un área especifica puede ser común en otra. Los eventos extremos no pueden ser atribuidos al cambio climático, ya que estos se pueden dar de manera natural, sin embargo, se espera que el cambio climático pueda incrementar la ocurrencia de eventos extremos. Ejemplos incluyen: inundaciones, sequias, tormentas tropicales y olas de calor. Nicaragua es el cuarto país en el mundo que ha sido más afectado por los eventos climáticos extremos entre 1994 y 2013, de acuerdo con el Índice de Riesgo Climático Global 2015, publicado por la organización medioambiental alemana German Watch.Es la propensión o predisposición de un sistema a verse afectado negativamente. Implica una variedad de conceptos incluyendo sensibilidad o susceptibilidad al daño y la capacidad de enfrentar y adaptarse a efectos adversos del cambio climático, incluida la variabilidad climática y los fenómenos extremos. Puede ser vulnerabilidad de ecosistemas, económica, en la salud de las personas, del recurso hídrico, entre otros.Un ejemplo de vulnerabilidad económica es, el caso del evento extremo ocurrido en Piendamo (Cauca), los campesinos cultivadores de café son altamente vulnerables al vendaval y la granizada, dado que no tienen el dinero suficiente para recuperar sus cultivos y mantener sus ingresos después de ocurrido el fenómeno extremo.Adaptación, es el proceso de ajuste al clima actual o esperado y a sus efectos. En sistemas humanos, parece estar moderada por el daño o por el aprovechamiento de oportunidades benéficas, buscando reducir la vulnerabilidad. En sistemas naturales, la intervención humana puede facilitar el ajuste al clima esperado y a sus efectos.Un ejemplo de adaptación al clima actual de paramo por parte de los frailejones (plantas) es la disposición de sus hojas en roseta, abundante pubescencia (similar a vellos) en las hojas, mantener las hojas muertas en su tronco, entre otras, que le permiten superar el frio. Por su parte, el ser humano que vive a orillas del mar, lagunas o ríos, construye su casa como palafito, es decir, apoyada sobre pilares que les permite protegerse de las variaciones en el nivel del agua.La capacidad de adaptación, es la capacidad de sistemas, institucionales, humanos y otros organismos para ajustarse al cambio climático (incluida la variabilidad climática y los cambios extremos), a fin de moderar los danos potenciales, aprovechar las consecuencias positivas o soportar las consecuencias negativas. Un ejemplo de ello, es la capacidad que tenga un agricultor para enfrentar la escasez de agua, en donde tendrá mayor capacidad, aquel que haya diseñado en su vivienda un sistema de recolección y almacenamiento de aguas lluvias. Por su parte, los animales y las plantas que tienen amplio rango de distribución tendrán mayor capacidad de adaptación que aquellas restringidas a hábitats específicos, teniendo en cuenta que la adaptación de la fauna y la flora responde a procesos evolutivos que, generalmente, incluyen largos periodos de tiempo.Una medida de adaptación, es la práctica de identificar opciones para adaptarse al cambio climático y evaluar tales opciones en función de criterios como disponibilidad, beneficios, costos, efectividad, eficiencia y factibilidad. Como ejemplo, se encuentra la implementación de prácticas de conservación de suelos en terrenos susceptibles a la erosión, diversificación de cultivos, implementación de sistemas agrosilvopastoriles, entre otras. Para especies de flora y fauna nativa, se incluye la protección de continuidades o conectividades de hábitat, la restauración ecológica y la conservación de bancos de germoplasma.Anticipada (proactiva, planeada, preventiva): es la que se produce antes de que puedan observarse los impactos del cambio climático.Reactiva: Adaptación que se produce después de haberse observado los impactos del cambio climático.Autónoma: esta adaptación no es una respuesta consciente a los estímulos climáticos, sino que es provocada por cambios ecológicos en los sistemas naturales, cambios en el mercado o en el bienestar de los sistemas humanos. También se denomina adaptación espontanea.Planificada: resulta de una decisión política deliberada, basada en la comprensión de que las condiciones han cambiado o están por cambiar y que se requieren medidas para volver a un estado deseado.Privada: Adaptación iniciada y ejecutada por personas, familias o empresas privadas. La adaptación privada suele responder a un interés fundado de quienes la realizan.Pública: Adaptación iniciada y ejecutada por cualquier nivel de gobierno. La adaptación publica suele orientarse a necesidades colectivas finalmente, el manejo adaptativo, es un proceso continuo de planificación, implementación y modificación de estrategias para el manejo de los recursos buscando enfrentar la incertidumbre y el cambio, implica ajustar enfoques en respuesta a observaciones de sus efectos y cambios en el sistema generando efectos de retroalimentación y otras variables.Es el nivel en que un sistema o especie resulta afectado, ya sea negativa o positivamente, por estímulos relacionados con la variabilidad o el cambio climático. El efecto puede ser directo (p.e. cambio en los rendimientos de los cultivos por cambio de la temperatura media) o indirecto (p.e. cambios en la disponibilidad del forraje que afectan la producción ganadera). Un ejemplo de ello, son los glaciares, altamente sensibles al incremento de la temperatura que determina la fusión del hielo como efecto adverso.Es la presencia de personas, medios de vida, especies o ecosistemas, servicios y recursos ambientales, infraestructura o bienes económicos, sociales o culturales, en lugares que pueden verse afectados negativamente, es decir, por su ubicación en una zona geográfica que puede sufrir impactos determinados por la variabilidad o el cambio climático. Un ejemplo de ello, es la exposición a inundaciones de los habitantes de la ciudad Rama-RAAS, en Nicaragua.El fenómeno de El Nino -Oscilación Sur (ENOS) es un patrón climático recurrente que implica cambios en la temperatura de las aguas en la parte central y oriental del Pacifico tropical. En periodos que van de tres a siete años, las aguas superficiales de una gran franja del Océano Pacifico tropical, frente a las costas del norte de Perú, Ecuador y sur de Colombia, se calientan o enfrían entre 1° C y 3° C, en comparación a la temperatura normal. Este calentamiento oscilante y el patrón de enfriamiento, es conocido como el ciclo ENOS (ENSO por sus siglas en Ingles), afectando directamente a la distribución de las precipitaciones en las zonas tropicales y puede tener una fuerte influencia sobre el clima en los otras partes del mundo. El Nino y La Nina son las fases extremas del ciclo ENOS; entre estas dos fases existe una tercera fase llamada Neutral. El nombre de El Nino (refiriéndose al niño Jesus) fue dado por los pescadores peruanos a una corriente cálida que aparece cada año alrededor de Navidad. Lo que ahora llamamos El Niño les pareció como un evento más fuerte de la misma, y el uso del término se modificó para hacer referencia solo a los hechos irregularmente fuertes (INTA, consulta 2014).Como ejemplo se puede citar lo publicado por FIDA 2010: \"En Nicaragua el cambio climático está incidiendo en los sistemas de producción agrícola, pecuaria, en los ecosistemas y sobre la población del país. La información disponible refiere que la temperatura ha variado de 0.2 a 0.9 grados centígrados en los últimos 30 años. Los aumentos de temperatura van desde 24.5 0C (década 1961-1970) hasta 25.9 0C (década 2001-2011) ","tokenCount":"3934"} \ No newline at end of file diff --git a/data/part_6/1136138015.json b/data/part_6/1136138015.json new file mode 100644 index 0000000000000000000000000000000000000000..59f3e11050bd0a57cf468e8c1916dde4f956c291 --- /dev/null +++ b/data/part_6/1136138015.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"4d49607e2989440fbfb82c82860463bc","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/5c435f76-893f-45a1-b4a5-6da2e7a2f0eb/retrieve","id":"-332812862"},"keywords":[],"sieverID":"b137acab-be28-4ee2-b935-4ac196016b20","pagecount":"17","content":"Heans are produced prlmarlly by small scale farmers in Uganda (Bank of uganda, 1966). The productlon systems are dlverse and often complexo Uganda's favorable climatlc and edaphlc condltlons allow for a wide range of crops to be grown and beans are found grown In pure stand and in assoclatlon with various cereals, root crops, vegetable crops, tobacco and bananas.These assoclatlons are most often intercropping systems, but also relay lntercropplng and rotatlons are found.The unreliability of input and output markets necessitates flexibility in the production systems. The farmers' bean production objectives are several and vary in relative importance, from farm to farm, district to district and year to year. Resources aval1able to sroall farmers are often few and diverse and these must be allocated between different production activlties to obtaln sufflclent but stable productlon. These characterlstlcs of sroall scale bean production systems lend to their complexlty and dlverslty and underly the need for worklng closely with farmers to understand their decision making envlronment, to ldentlfy feasible technologlcal alternatives, and to evaluate these alternatives.The complexlty and dlversity of bean production systems in Uganda underlies the need for farmer participation In research.Farmers can provide local knowledge of their objectlves and produqtion systems and can contrlbute to the evaluation of technologles in 11ght of their objectlves and constraints.Through on-farro experlmentatlon, technologies can be evaluated undel:: falmer:5' envlronmental .'1I1,j ma.nager 1,'11 condl t lon:5. Farmer:5, in any case, make the final declalon on the worth of a technology and the aooner they can be involved in the evaluation of alternative technologles, the more efficlent the research process la l1kely to be. In response to the fal1ure of upland farmers to adopt lmproved cropplng patterns, farmers were 1nvolved in the ldentlfying, analyzlng and solv1ng of systems product1on problems in the Phllippines (Lightfoot et al, 1967 andLightfoot et al, 1988), but rather than addresslng the crop components of the system, they dealt with soil fertillty and the invasion of rotatlonal fallow by Imperata cylindrlca. In India, researchers collaborated with farmers to establlsh criteria for screening upland rlce varletal material (Maurya et al, 1988) select on the conduct of the trials and to evaluate the results of the trials (Norman et al, 1988). In Colombia, Ashby (1966) In each of the dlstrtcts, three or four villages were selected for a dlagnostlc survey to be eonducted by researchers, together wlth selected extenslonlsts. A one-day tralning course on lnterviewing techniques was held, and a questionalre was elther formulated or revised on that day. lnterviews were held wlth 27 to 40 farmers per dlstrlet, over a perlod of 2-4 days.The interviewers dlscussed thelr ftndlnga at the end of each day.After the surveys, the information collected was analyzed and interpreted.Constralnts were listed and prioritized, posslble solutlons were identified.On-station and on-farro experlments problems.were designed whlch focused on the important More recently, surveys have been eonducted which were dlrected to speciflc aspects of bean produetlon. ","tokenCount":"481"} \ No newline at end of file diff --git a/data/part_6/1153773042.json b/data/part_6/1153773042.json new file mode 100644 index 0000000000000000000000000000000000000000..ee1e10d7cdd9ffafc44de5a6fcdf1145c475c424 --- /dev/null +++ b/data/part_6/1153773042.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"c9b98390d01905b91490c7bec0a655fa","source":"gardian_index","url":"https://publications.iwmi.org/pdf/H041933.pdf","id":"845644868"},"keywords":["проф. В.А. Духовного","д-ра В.И. Соколова","д-ра Х. Мантритилаке от теории к реальной практике. Опыт Центральной Азии. Под ред. проф В.А. Духовного","д-ра. В.И. Соколова","д-ра. Х. Мантритилаке -Ташкент: НИЦ МКВК","2008. -364 с Духовный В.А.","Соколов В.И.","Мантритилаке Х.","Казбеков Ж.","Анарбеков О.","Мирзаев Н.Н.","Пинхасов М.А.","Алимджанов А.","Мухамеджанов Ш.Ш.","Нерозин С.А.","Галустян А.Г.","Хорст М.Г.","Стулина Г.В.","Зиганшина Д.Р.","Масумов Р.","Кадыров А.А.","Умаров П.Д.","Бегимов И.","Хегай В.В.","Тучин А.И.","Жерельева С.Г.","Рощенко Е.М. и др. ISBN 9965-32-627-4 ББК 26.22"],"sieverID":"7f193069-58be-4340-b12a-36dc49112a13","pagecount":"14","content":"Настоящая публикация предназначена для широкого круга специалистовводников, включая лиц, определяющих водную политику и принимающих решения, которые формируют ход и содержание современных реформ в сфере руководства и управления водой.Публикация также предназначена для широкого круга представителей гражданского общества -заинтересованных сторон в надлежащей реализации водохозяйственных реформ. Содержание книги позволит читателям проникнуться пониманием, что человечество и природа практически повсеместно на Земле столкнулись с серьезными проблемами вокруг воды. Эти проблемы сегодня невозможно эффективно решать с использованием привычных, сложившихся в последние десятилетия традиций, структур руководства и методов управления водой. Интегрированное управление водными ресурсами (ИУВР) рассматривается здесь как новый и потенциальный подход решения указанных проблем. Настоящая книга обобщает первые опыты внедрения этого нового подхода в Центральной Азии.• организация учета оросительной воды на границе фермерских хозяйств;• технологии использования оросительной воды;• повышение эффективности и продуктивности воды и земли;• виды и нормы внесения удобрений;• борьба с вредителями;• оптимальные сроки и виды агротехнических мероприятий;• повышение урожайности сельхозкультур.Кроме того, консультативной службой проводится оценка обеспеченности и стабильности водоподачи по различным уровням каналов и ассоциаций водопользователей. Развитие опытнодемонстрационных участков для изучения и отработки методических подходов для решения прочих -не рассмотренных ранее проблем, имеющим место в фермерских хозяйствах.Социальная мобилизация -это процесс, посредством которого все заинтересованные стороны привлекаются к участию в управлении водными ресурсами, принятии ключевых решений по руководству и обеспечении надлежащего состояния инфраструктуры [7]. Социальная мобилизация организуется как консультативный процесс, где все заинтересованные стороны вырабатывают общее понимание проблем и потребностей в сфере управления водными ресурсами и ведут постоянный широкий диалог по согласованию коллективных действий, направленных на решение, этих проблем через создание общественных органов управления водными ресурсамитаких как АВП, СВК и совместных государственно-общественных партнерств как ВКК.Социальная мобилизация является непрерывным процессом, когда специалисты по социальной мобилизации встречаются с общественностью, фермерами, представителями водохозяйственных организаций, руководителями местных администрации и т.д. Специалисты объясняют организационную структуру, необходимую для ИУВР, шаги усовершенствования организационной структуры управления, процедуры создания того или иного общественного органа, которые в дальнейшем будут участвовать в руководстве системой или АВП. Разъясняется суть передачи части полномочий от государства в руки самих водопользоваталей, роль государства в управлении водными ресурсами. Социальная мобилизация также является процессом, где учитываются мнения всех заинтересованных сторон. Это -так называемый двусторонний диалог, где рассматриваются и учитываются новые идеи заинтересованных сторон, дается возможность подумать и представить свои идеи, которые фиксируются в отчетах и в дальнейшем служат основой для уточнения стратегии общественного участия.В этом разделе излагается опыт СМИР 6 по третьей фазе проекта «ИУВР-Фергана». Если на начальных этапах проекта работы по СМИР были направлены на создание единичных общественных организаций -пилотных АВП, Управлений каналов и комитетов водопользователей, то в третьей фазе задачи и стратегия СМИР носили более интенсивный и расширенный характер. В частности, мероприятия по СМИР были направлены на укрепление ранее созданных органов по управлению водой и распространению опыта пилотных объектов на большую территорию с конечной целью обеспечения устойчивости создаваемых организаций.Организационная структура полевых работ по мобилизации приведена на рисунке 4. 26. Для обеспечения эффективности работ было решено увеличить число полевых консультантовмобилизаторов под руководством единого координатора. В задачи последнего кроме органи-зационных работ входила выработка единого подхода и координация действий со всеми ключевыми лицами и заинтересованными сторонами.В начале каждого года, работники проекта выезжали и консультировались с заинтересованными сторонами по вопросам правильной, эффективной и интенсивной организации СМИР на каждом пилотном канале. Все рекомендации и предложения записывались. На основе анализа поступивших предложений по улучшению работ и задач проекта разрабатывалась стратегия осуществления работ по СМИР применительно к каждому каналу. По результатам разработки в каждой стране с командой по СМИР проводился рабочий семинар, где каждый консультант имел возможность выступать и высказывать мнения по поводу предлагаемой стратегии, т.к. полевой и практический опыт мобилизаторов был всегда полезен в выработке планов работ. На основе уточненных и скорректированных в результате обсуждений генерального плана, каждый член команда СМИР разрабатывал индивидуальный план работ, который в течения года служил инструментом для мониторинга и оценки продвижения работ и деятельности команды.Содержание СМИР по странам может различаться в связи с местными особенностями, водохозяйственными и социо-экономическими условиями, но строится вокруг следующей целостной структуры:• Встречи с представителями ВХО, местных властей и водопользователей для общего представления и выявление зоны деятельности;• Обход обозначенной территории для выявления проблемных участков и встречи непосредственно с местными ВХО, властями, первичными водопользователями, существующими АВП и вторичными водопользователями; СМИР через сотрудничество с местными учебными заведениями. Для обеспечения долгосрочной устойчивости результатов проекта «ИУВР-Фергана» было решено передать накопленный положительный опыт и материалы проекта в местные учебные заведения, специализирующиеся в подготовке специалистов водного хозяйства среднего и высшего звена. Для достижения этой цели были установлены рабочие отношения и подписаны соответствующие меморандумы о взаимопонимании с высшими и средне-техническими учебными заведениями (Мархаматский гидромелиорационный колледж, Ошский сельскохозяйственный институт, Ходжентский филиал Таджикского технического университета). Были организованы семинары по обмену опытом, поездки на пилотные объекты, переданы все технические материалы и наработки, созданы рабочие группы из педагогического состава учреждений для дальнейшей адаптации опыта в учебные пособия и внедрения их в учебный процесс.В 2007 году вышеперечисленные учебные заведения ввели в учебный процесс дополнительные занятия по ИУВР (50 академических часов -20 часов теоретических и 30 часов практических занятий). В 2007 году 50 студентов 2 и 3-го курсов проходили производственную практику на пилотных каналах, помогали мобилизаторам, АВП и гидротехникам. Были также установлены рабочие отношения с Бишкекским и Душанбинским аграрными университетами. Подписаны соответствующие меморандумы по сотрудничеству, также переданы материалы проекта.С 2007 года были начаты мобилизационные работы по распространению опыта на Правобережный канал в Кыргызстане. Идет работа по гидрографическому анализу водохозяйственной обстановки зоны канала. Начаты работы по мобилизации среди руководящих работников по созданию единого управления систем канала. Ведется разъяснительная компания среди ключевых субъектов по подготовке водопользователей для создания СВК.Положительный опыт социальной мобилизации, приобретенный на пилотных объектах через совместные семинары и тренинги передаются к зонам 2-х пилотных трансграничных малых реках (ТМР) -Шахимардансай и Ходжабакиргансай. Распространение принципов ИУВР будет организовано по аналогии с проектными мероприятиями на каналах; и стратегия социальной мобилизации будет реализована на следующих уровнях: Система (ТМР и ирригационная система), АВП (и местные сообщества), и ниже уровня АВП (распространение методики проекта через местных консультантов).Развитие АВП через социальную мобилизацию. Если задача социальной мобилизации на 2-ой фазе была направлена на создание единичных, т.е. пилотных АВП, то на 3-ей фазе она фокусировалась на всеобъемлющее и экстенсивное распространение опыта пилотных проектов на всю территории пилотных каналов, тогда, как все новые АВП создавались преимущественно административным методом. Поэтому все мобилизационные работы направлены на повсеместное развитие АВП вдоль пилотных каналов аналогично пилотным АВП. Для этих целей были подобраны полевые команды по СМИР, обученные новой стратегии развития АВП. Стратегия по странам отличалась в связи с местными особенностями, водохозяйственными и социально-экономическими условиями. Мобилизационные мероприятия включали в себя такие работы как создание гидрографических АВП, реорганизация административных АВП в гидрографические, укрепление созданных АВП, обучение персонала (организационные вопросы, управление водными ресурсами в АВП, бизнес план), активизация советов АВП и их участие в работе полевых подразделений или в самих СВК, создание эффективных ГВП. В создании и реорганизации АВП участвовали члены СВК, БУИС, БУВХ, специалисты районных хокимиятов, хукуматов, местных администраций, председатели махаллинских комитетов сельских населенных пунктов, сельских управ, руководители АВП, МТП, специалисты гидроучастков канала, и сами водопользователи -фермеры. Все мероприятия координировались и осуществлялись совместно с областными исполнителями и полевыми консультантами НИЦ (см. табл. ","tokenCount":"1135"} \ No newline at end of file diff --git a/data/part_6/1177524449.json b/data/part_6/1177524449.json new file mode 100644 index 0000000000000000000000000000000000000000..baeee72580952dc6c7a24dbc15fc5ee8cd0dcd60 --- /dev/null +++ b/data/part_6/1177524449.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"0084acec99aea6afb1ad8e7dabb1897b","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/6afb6394-0f72-41d6-9e41-ab51c0cca28b/retrieve","id":"1122239142"},"keywords":[],"sieverID":"181b7029-1bdf-4082-86c3-9e4723c8b6f1","pagecount":"65","content":"ILRI would appreciate being sent a copy of any materials in which text, photos etc. have been used Acknowledgements: The authors thank Rosekellen Njiru and for Itseng Malao for their contributions to organizing this meeting.In November 2023, the wider COHESA project convened in Pretoria for the project's first biennial conference. Tasked to review progress, share lessons, insights and innovations and to formulate priority actions for the coming two years, eighty-five people participated from all 12 project countries.The first day zoomed in on activities in the four work packages -looking first, package by package and then country by country. These collective exercises revealed project-wide strengths across the countries, such as the collaborative networked approach, capacities developed, inclusion of diverse institutes and issues, the Netmapping of actors and interests, the emerging evidence base, experimental approaches like sandpits to identify solutions, work on One Health platforms and outreach to schools, and the flexible funding approach. The country team interactions revealed a wealth of activity as well as some important challenges and lessons encountered. Beyond general issues around lack of One Health awareness, participants pointed to the dangers of institutional and sectoral silo's, One Health funding challenges at country level, and, sometimes, missing key groupssuch as from environmental and ecohealth.Thematically, the second and third days focused on One Health governance (and platforms) and on One Health outreach to schools. For both topics, country cases were presented as starting points to identify opportunities and priorities for further work. Success in the governance space was frequently framed around strategy and policy development, advocacy to policy communities, effective coordination and leadership and financing for One Health. Pathways towards such successes were identified as critical success factors (collaboration, inclusion, ownership …), and what to avoid (duplication, fragmentation, silo's …). For the schools agenda, integrating One Health through interaction with teachers and educational curriculum and content design was highlighted; a key priority is to work with Ministries of Education as well as other groups with strong interests in early childhood education. The question as to when such efforts can best deployed -the target age groups -was discussed by not fully answered.The event timing half-way through the project offered a good opportunity to look to the coming years, accelerating activities and results and also identifying critical elements of 'exit strategies' at country level. Beyond fundraising for continuation or complementary projects, participants emphasized in-country advocacy and alliance/network building as important to sustain momentum post-COHESA. They also identified a number of 'products' that need to be in place to ensure that key evidence, messages and collaborations continue to have outcomes beyond the project end.Finally, a reflection team gave daily feedback and, in the final session, shared their perspectives on COHESA strengths, weaknesses, opportunities and threats. They observed strengths around the network itself, the commitment and engagement of participants and the diversity represented. Weaknesses highlighted included the visibility of the project as well as concerns around sustainability, and monitoring and evaluation. Opportunities mentioned included the potential for some elements to be replicated and scaled elsewhere, donor interest in these issues, and an urgency to build on and really exploit the power of the network. Threats identified included, particularly, the resistance of many actors to change that calls for innovative approaches from the project. Around these discussions, participatory sessions were organized to facilitate exchange of ideas and innovations across the network. Three sessions provided opportunities for participants to hear inspirational presentations on wider topics.As we increasingly recognize the inter-connecting factors that influence the health of people, animals and the environment, 'One Health' -defined as an integrated, unifying approach that aims to sustainably balance and optimize the health of people, animals and ecosystems -is seen as a very promising way to frame and take action at different levels -international, regional, national and local.Essentially, the argument for One Health says that successfully reducing future health risks and impacts for people and livelihoods, as well as for animals and ecosystems, is most likely to come when we apply diverse expertise across public, veterinary and environmental health.The importance of adopting the One Health approach has been reinforced by lessons from recent disease outbreaks including COVID-19, Ebola and avian influenza. In addition, One Health thinking is embedded in current efforts to reduce the spread of antimicrobial resistant pathogens, to ensure food safety, reduce water and waste-borne contamination, manage human and livestock interactions with wildlife, and reduce aflatoxin contamination in crops and livestock products, to name only a few examples. It can also be seen in structural efforts to establish 'One Health' collaborative, cross-departmental organizational structures. To succeed, all require a triple 'input' of public, veterinary and environmental health expertise together with an understanding of the wider systems involved.While the overall approach has been around for some time, implementation of genuine One Health faces several challenges, key being the many sectoral, domain, disciplinary, academic, organizational and investment silo's that limit necessary cross-communication and integration of efforts and which ultimately segregate people and ideas, restricting the development of integrated, comprehensive solutions.With financial support from the EU, funded through the OACPS Research and Innovation Programme, the 'Capacitating One Health in East and Southern Africa' (COHESA) project is tackling key One Health capacity gaps in the region. It is implemented through enhancing the knowledge base for research and policy-making, strengthening national and subregional cross-sectoral collaboration, building academic and research capacities and One Health education, and growing the abilities of actors to deliver One Health solutions.COHESA is led by the International Livestock Research Institute (ILRI), the French Agricultural Research Centre for International Development (CIRAD -Centre de coopération internationale en recherche agronomique pour le développement) and the International Service for the Acquisition of Agri-biotech Applications (ISAAA).Convened by ILRI and hosted by the University of Pretoria, this first biennial conference brought together project partners and 'multipliers' (local project implementors) to take stock of progress and set priorities for the coming years. Over the three days, 85 people (41% women; 59% men) from 13 countries participated in reviewing progress and activities, discussed experiences around OH governance and in schools, shared innovations and identified activities for the coming years.Participants were welcomed by Hung Nguyen-Viet, Program Leader at ILRI. Additional remarks were provided by Margaret Karembu, ISAAA-Africenter; Alexandre Caron, CIRAD; and Gerard den Ouden, OACPS Research and Innovation Programme.Theo Knight-Jones, ILRI Principal Scientist and COHESA lead introduced the objectives and structure of the COHESA project with its four work packages:1. OH Knowledge sharing: Increased relevance of One Health research and policies in Eastern and Southern Africa. 2. OH Governance: Enhanced national and subregional cross-sectoral collaboration between government entities with OH mandates and OH stakeholders across society. 3. OH Education and research: Educational and research institutes equipped to train the next generation workforce in tackling OH issues. 4. OH Delivery: Increased capacity of government and non-governmental stakeholders to identify and deliver OH solutions to final beneficiaries.After an introductions exercise, the facilitator introduced the overall agenda of the three days in relation to the formal objectives of the workshop:• Progress and Planning: Participants will gain insights into the progress achieved, highlighting successes, challenges, and plans for further development.• Knowledge Sharing: Participants will exchange perspectives, share experiences, and explore innovations aimed at addressing complex health challenges across human, animal, and environmental spheres. • Synergy and Collaboration: Participants will discuss shared goals and challenges, leading to synergies and collaborative opportunities identified and future initiatives inspired.In terms of process, the facilitator emphasized the participatory nature of the event with group interaction and just a few presentations. Throughout the process, key points would be captured on notes and flip charts, interactive sessions would be followed by quick sense-making by two participants -Carol Mufana and Clovice Kankya; while a reflection team comprising Adana Mahase-Gibson, Lucinda de Araújo, Brian Perry and Gerard den Ouden provided regular daily inputs as well as a closing synthesis.The core activities of the project are delivered axcross the countries through four work packages. After a short introduction by Theo Knight-Jones (ILRI), each package was briefly introduced covering: ambitions and outcomes; progress, results, plans; insights, lessons, challenges; and a call to action. After all the presentations -designed to refresh all participants, the table groups carried out a SOAR exercise to reflect on project overall and specific work package progress.Ambitions and outcomes• Determine the OH status in research and innovation, governance, education, and implementation • Where are strengths and gaps? -how can gaps be filled • Determine where gaps align with project objectives • Determine where COHESA can align with ongoing OH projects/initiatives Progress, results, plans• 11 baseline assessments complete inclusive of reports• Somalia about to start as they recently joined the project • Netmapping completed in 9 countries (original plan for 4)• ISAAA supported this with train the trainer events (2) and assisting all countries to implement and report on findings Ambitions and outcomes• COHESA is a project about OH institutionalization (= setting up the framework from national to local level for OH to be operationalized) Following the presentations, participants reflected on the project's perceived strengths, opportunities, aspirations and results. These are presented in the tables below. The afternoon of the first day was organized around the experiences of the countries. For this exercise, participants formed country teams. Members prepared summary information on each work package -highlights plus 3 to 5 activities, lessons, challenges -in advance of the conference that could be shared as insights with other countries in a peer to peer learning process.During the exercise, some team members remained at the table to share insights with people from other countries, while other team members visited other country teams seeking insights to include in their own plans. Through this exercise, team members from all countries engaged with all other country teams.The images below show the framework that each country populated. This is followed by the summary material compiled by each country -the detail as well as the summarty 'slides' used for the exercise. Annex 5 presents all the individual highlights, activities, challenges and lessons by work package.. Learnt that there is still a big gap among \"OH-sectors\", despite acknowledgement of the existence of the OH platform. Learnt that a District that adopted and actualised OH early was able to effectively respond and handle the Anthrax situation better. Wrapping up discussions on work packages and country lessons and insights, participants formed small groups to identify: 1) powerful ideas or lessons learned during the day and 2) important actions to take.Important actions The morning of the second day focused on lessons and experiences with 'One Health governance'.The main components were:1. A presentation of Netmapping findings by Bibiana Iraki (ISAAA-Africenter) 2. Short country cases from Gabriel Shirima (Tanzania), Namibia: Simon Angombe (Namibia)and Joshua Onono (Kenya) 3. A talk show/panel of regional actors moderated by Kristina Roesel (ILRI) in conversation with: Musonsa Ngulube (UNEP), Gerald Mucheru (FAO), Hardwick Tchale (World Bank), Gaolathe Thobokwe (SADC) and Fahari Gilbert Marwa (EAC). 4. An exercise by participants in country groups exploring 1) success for OH governance in their countries and 2) Critical Success Factors to to achieve this (do's and don'ts)Bibiana Iraki (ISAAA-AfriCenter) shared findings from COHESA Netmapping exercises.She explained why COHESA uses it -to understand stakeholder relationships and connections, emphasizing its purpose as a participatory, reflective tool for advanced problem solving and stakeholder engagement; its importance contributing to effectiveness in complex multidisciplinary networks such as OH and its credibility.For COHESA, it helped to identify three emerging scenarios:• Working towards operationalisation of OH • Institutionalization of a national OH entity • Working towards integration of OH issue-based TWGs; national OH entity; Institutionalized Findings from across the countries revealed some commonalities:• Institutionalization key to fully operationalize OH. Ensures effective coordination and accountability at national level • Limited advocacy happening with key actors needed to institutionalize OH entities i.e. Prime Ministers Office, Office of the President, Legislators and Ministry of Finance • Collaboration extensive between key-line ministries and technical actors. However, they tackle specific OH issues or happen unconsiously -except for MoH and MoA • MoE and wildlife ministries PLUS some key regulatory agencies not as active • Limited collaboration with key drivers needed to deliver and adopt OH solutions e.g. grassroots groups, local governments, private sector • Funding is available but for sectoral OH issues -contributing to poor integration. • OH agenda is largely donor driven -yet limited engagment with Ministries of Finance. Grants go through development partners and not directly to line ministries. • OH approach is an abstract concept that is practised in ivory towers -experts speaking among themselves • Advocacy is absent/limited among key actors. Key for increasing importance/value of OH and achieveing goal but identified as an area of weakness • Lack of integration not just within government but also across OH initiatives She also highlighted som critical success factors for effective operationalization of OH governance: Effective communication skills, conflict resolution, negotation skills, financial accountability and knowledge sharing.The three presenters were asked to give updates on the current OH governance platforms in their countries, covering: current status, prospects and plans, lessons and insights, as well as a critical success factor.Current status • Kenya's OH office formed between line ministries of human and animal health This session was introduced by Florence Mutua (ILRI) and followed by 4 short country cases and an interactive session in which participants were asked to reflect and identify priority actions in this area.Mutua first introduced this area of work as looking towards a future with professionals who are better prepared to tackle OH issues and exposed 'early.' Some of the questions are around this notion of earliness: How early is early and which level/ grade do we focus on? Further, are there models we can tap into, who else is working on this space, what content is appropriate (for primary, secondary schools, others), which approaches suit different ages and contexts and where should the focus be -extra-curricular, in the curriculum, via teacher education, etc.?The four cases introduced different approaches and experiences, setting out for each: the ambitions and outcomes; the actors and their roles; progress, results and plans; insights, lessons and challenges; and a call to action.Shauna Richards introduced a case where primary and secondary schools engaged with One Health through learning about safety around animals. The project trained primary (2022) and secondary ( 2023) school teachers in one sub-county, covering:• learning about One Health, with examples relevant to the sub-county/student education level, and how to integrate One Health into the secondary school curriculum; and • understanding how safety around animals is related to One Health, with examples of prevention of zoonoses (with rabies as a specific example), prevention of injury from animals and improving animal welfare and the health of people and animals while considering environment health.Teachers were trained on technical materials and provided with lesson plans and teaching materials covering 1 : • Ideally continue at a few diverse sites in Kenya to ensure material is appropriate • Bring M&E + report to Ministry of Education to consider about fully integrating into curriculum in future • Trainers can be students from Higher Education Institutions for in service teachers; new teachers to learn in their program.Catherine Wood reported on a collaboration between the Lilongwe University of Agriculture and Natural Resources (LUANAR) and a local NGO -Ladder to Learning -to extend One Health in Primary Education in Malawi. The project worked through existing literacy hubs, learning spaces and libraries, mentoring programs and digital skills training, involving the LUANAR veterinary students association in content development, delivering and mentoring for the children.Xavier Edziwa explained how the University of Zimbabwe is infusing the One Health concept in preservice teacher education.As part of the assignment of the UZ Faculty of Education to lead a Review of Teacher Education Curriculum, this provided an opportunity to introduce OH in the curriculum, aiming to:• Conduct a needs assessment at five teacher training colleges to establish the level of preparedness for the adoption of OH as part of the school curriculum. • Devise strategies to infuse One Health into the school curriculum, particularly through teacher development programmes that are currently under review (Teacher Education Curriculum Transformation).The assessment revealed that the current teacher education curriculum has some OH themes already, though lacking an interdisciplinary approach and not strongly visible.The next steps are for the implementing institutions to develop specific learning outcomes and instructional strategies that incorporate OH principles across identified subjects.The main challenge is that the pace of implementation will be determined by the rolling out of the teacher education curriculum transformation.The Faculty of Education has started engaging with participating colleges to start making OH more visible and it will run capacity building workshops on identified OH knowledge and skills gaps for teacher education college lecturers.Paul Buyugu introduced the work of 'One Health Lessons', an global initiative to inspire children and adults around the world to value One Health. Focusing on its Train-the-Trainer Program that certifies lesson leaders to provide training for adults, communication skills and age-appropriate lessons from age 6 and upwards 2 .Following the presentations, participants identified some priority actions in this area for COHESA to take forward (see box below).• Advocacy for the infusion/integration of OH at the highest level of institution with this mandate On day 3, to facilitate discussions and collaboration among participants, an open space session was held where individuals shared ideas where they wished to collaborate with colleagues. The champions and topics in this session were:Noting that the project is already half through its cycle, participants brainstormed actions that can be planned and implemented now to ensure effective 'exits' for COHESA activities. Recognizing also that the legacy and products of COHESA will continue after the end of the project, and indeed that we want them to have impacts, the groups also identified some attributes of products that will make them 'future-ready' -for a post-COHESA phase.Advocate for multipliers to be included on the national OH platforms or OH advisory committee Advocate to increase government budgets for OH -cost benefit analysis, demonstrated public good Align COHESA OH activities to government objectives Build co-ownership of all activities Create another project to sustain existing networks and collaborations and benchmarking/best practices Develop new collaborations / partnerships Develop sustainable cross sectoral collaborations using the existing OH platforms at local and regional levels Disseminate products through media cafes, ministerial websites, co-authored/ownership Document and begin implementing the exit strategy now Engagement (PPP) and advocacy Establish legal framework for OH -link it with budget framework Explore alternate funding mechanisms Facilitate national OH platforms and incorporate other stakeholders Find mechanism to ensure continued stakeholder engagement Hold Conference/Seminar/Symposium in individual countries annually Identify and establish partnerships with other OH related entities Identify parliamentarian to be a OH champion Increase iInvolvement and commitment of government, private sector and community in OH activities Institutionalise OH across borders Integrate OH into government programs and plans Leverage the strengths of the existing network Mobilize additional funding for continuity of specific activities Optimise search for funding opportunities Organise an evaluation of the project implementation Organise an exit conference with the relevant stakeholders Package the products such as policy briefs, white papers, strategies to get buy in Project documentation tell the COHESA story Publicise OH benchmarks and encourage HEIs to utilise them with curricular development Raise country and government commitment to institutionalise OH activities Reach long term impact at community level by integration/transfer of OH principles at community levels Resource mobilisation for post-COHESA Run webinars Secure extra funding -Funding collaborative, Grant applications. Science Foundation Africa Strengthen existing OH platforms and initiate OH platforms where they don't exist Support government structures to secure internal/domestic funding Sustain relationships between academia and government agencies for evidence based policies eg updating future OH strategic plans Use COHESA as a benchmark /model for future OH projects Use network to fund future collaborative project Validate strategic plans and assign roles and responsibilities for uptake of activities by network partners Before the closing, the reflection team members shared their observations and recommendations in the form of a SWOT. ","tokenCount":"3310"} \ No newline at end of file diff --git a/data/part_6/1183470292.json b/data/part_6/1183470292.json new file mode 100644 index 0000000000000000000000000000000000000000..cda0d360e40b5039887f1c6527032b6b2e437a40 --- /dev/null +++ b/data/part_6/1183470292.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"9928c4eacb1061a06127e91f34dd4802","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/343d8354-e35c-4cce-b49f-328dc4639479/retrieve","id":"-2069341305"},"keywords":[],"sieverID":"7b13fedc-f1a0-4e8d-a1a0-a5854e506eca","pagecount":"36","content":"This report is based on a scoping study carried out on behalf of the Ethiopian Forage Seed Consortium from September 2022 to February 2023. Its purpose was to gain a better understanding of the way in which the forage seed system works, as a basis to make recommendations for improvement, or to suggest targeted interventions. About thirty stakeholders from the public and private sectors, and regulatory authorities, were consulted through a mix of structured interviews and informal discussions.The context of the study is that wider use of cultivated forages has been strongly advocated for many years, both to improve livestock productivity and to reduce the degradation of natural pastures by overgrazing. The poor availability and quality of forage seeds is routinely cited as a major reason why there has been poor adoption of cultivated forages and all concerned parties would like to remedy this situation.A previous study conducted by the International Livestock Research Institute (ILRI) in 2019 confirmed that there is no official certification of forage seeds due to the limited resources of regional quality control agencies and the priority given to maize and wheat seed, although regulations do exist for forage seed certification. This has prompted an interest in the use of quality declared seed (QDS) as an alternative system and a draft QDS scheme has been prepared as an activity of the Ethiopian Forage Seed Consortium for consideration by the regulatory authorities. This topic is addressed in Section 5 of the report and will be elaborated in a separate document.In the absence of a controlled seed multiplication system, there is a particular weakness in the transfer of early generation material from breeding stations to producers. As a result, variety identity is lost and most seed is grown and sold under the species name only. This raises concerns about the value of breeders' work and the variety testing/ registration system. It would benefit all parties if there were a greater awareness about varieties and more information available on this topic.Most seed production is undertaken by private companies who use their own land or contract growers and they monitor the seed crop fields. Some also use 'collectors' who are in direct contact with farmers in different parts of the country but this system is relatively unsupervised and may lead to confusion about the quality of seed, or malpractice. Regional bureaus of agriculture (BoA) also undertake some seed production as part of their extension work but this is not on a commercial basis. Substantial amounts of seed are produced through these various channels and primary production does not appear to be a constraint.Transactions are mostly conducted through public tenders announced by the various development actors and for which the producers submit competitive bids. Although this is a standard mechanism for government purchases, it has intrinsic risks for seeds because it may place undue emphasis on a low price at the expense of quality. This risk is increased by the lack of properly equipped laboratories for testing the quality of forage seeds within the official system or among other buyers. In practice, producers use informal testing arrangements to comply with tender specifications when they submit bids.Tenders announced by government agencies often have substantial financial contributions from official development agencies, who thus subsidize the seed supply system. Many non-governmental organizations (NGOs) use the same approach for their own projects. The additional funding available from these external sources masks the true market price of seeds based on production costs and margins; it may also raise concerns about the transparency of the bidding process. The tender system restricts the development of direct market linkages so it is more difficult for producers to establish a reputation for product quality and to benefit from that. To summarize, the use of tenders for bulk seed purchase introduces many complications into the market and in fact there is no true 'market' for these seeds, apart from limited local retail sales by producers.Free distribution of forage seeds to farmers has been practiced for many years as an activity of projects supported by the government, by official development agencies and by NGOs. As a result, farmers are reluctant to purchase seed at a market price. However, the study has revealed examples of more direct connections between seed producers and forage users, which could provide alternative mechanisms of seed supply and help to establish a viable seed market.Farmers who grow forage crops find it difficult to make the direct domestic benefit/cost calculation unless they are connected to a dairy unit where increases in milk production can be measured. However, there are many urban and peri-urban small dairy units, which should sustain a market for fresh forage production as a cash crop if seed were readily available in appropriate pack sizes.Issues raised by this study have policy implications that should be addressed at a high level to resolve longstanding problems in the seed supply system. The challenge is to develop a sustainable market for forage seeds that will benefit producers and provide a regular supply of good quality seed to users, bringing benefits both to livestock keepers and to the environment.We believe our study and this report provide a good understanding of how the forage seed system operates and will serve as a baseline for any further work on this important topic. With this in mind, Annex 5 gives a summary of information gaps and suggests further actions.The problem of forage seed supply in Ethiopia has been recognized for many years and is well-understood by scientists and development professionals. There is a strong case for increasing the use of cultivated forages both to improve livestock feeding and to reduce pressure on the environment caused by overgrazing of natural pastures. The lack of good-quality seed is frequently cited as a constraint to increasing the use of cultivated forages by livestock keepers and by farmers who could grow these as cash crops.In 2019 ILRI carried out a study for the ACDI-VOCA Feed Enhancement for Ethiopian Development (FEED) III project to develop a certification scheme for forage seeds. The clear conclusion from this was that all the necessary regulations for such a scheme are already in place but they are not being used. Reasons for this include the complexity of the current seed standards and limited capacity of the regional certification authorities, particularly for seed sampling and testing. In practice, the certification of cereal crops, especially maize and wheat, is the priority and more specialized crops such as forages are overlooked. In this connection, the use of QDS was suggested and discussed in the final report 1 and in a policy brief 2 . Stakeholder workshops held at the end of the study confirmed these constraints and the need to find more pragmatic approaches to quality assurance 3 .Another positive initiative of the FEED III project was to assist the establishment of a Forage Seed Production and Marketing Association (FSPMA) which would bring together the key actors as a more organized group to promote their common interests and strengthen linkages in the marketing chain. It took some time to complete the official registration but the inaugural meeting of the association was held in August 2021 with a total of 10 members and it is hoped that others will join. FEED III closed in 2020 and there was no follow-on project. To maintain the momentum and interest that had been generated by the study, ILRI established a Forage Seed Consortium to facilitate communication and coordination between the various concerned parties. This coincided with the arrival of the COVID-19 pandemic so all meetings of the consortium were held virtually for two years. However, membership gradually increased during that time and the key purpose of information exchange was achieved in these meetings. One important feature of the consortium is that it provides a forum for representatives of the public and private sectors with both crop and livestock expertise.The first physical meeting of the consortium was hosted by ILRI on 5 September 2022 with 18 members in attendance and two main items on the agenda. First, to introduce a scoping study of the forage seed supply system to be carried out for ILRI by a local consultant, Alemu Ejeta, with support from an international consultant, Michael Turner. Second, to present an overview of QDS, which has been much discussed as an alternative quality assurance mechanism for forages, given the recognized constraints of full certification, as noted previously. Another meeting of the consortium was held in ILRI on 2 February 2023 at which preliminary results of the scoping study were presented and the status of the QDS dialogue was updated. This report deals mostly with the forage seed supply 4 system (or trade) following collection of new data through a series of interviews with key informants. Section 2 provides background information on the seed system in Ethiopia and Section 3 then deals specifically with forage seed supply. Section 4 presents a discussion of the many issues affecting the forage seed system based on information collected in the survey. The development of a QDS scheme will proceed based on an analysis of existing documents and a needs assessment for the various actors. The background and current status of this work is covered in Section 5 and the key output will be a draft scheme submitted to the regulatory authority for consideration. Finally, Section 6 presents key conclusions and recommendations.4. The study and this report only address forage crops that are grown from true seeds; two important grass species, Pennisetum glaucifoliun (Desho) and Pennisetum purpureum (Napier) are vegetatively propagated and their supply to farmers (as cuttings or splits) were handled in different ways. The same applies to the Brachiaria hybrid 'Mulato' bred by the International Center for Tropical Agriculture (CIAT).2 The seed system in EthiopiaEthiopia has a rich experience of studies and investment in the seed sector dating back to a World Bank project in the early 1980s, which established the parastatal Ethiopian Seed Corporation. Even before that, there was Swedish support to a large integrated rural development project in the Arsi region (ARDU) that had a strong seed component. Over the past 40 years there have been many institutional changes and strategic initiatives aimed at strengthening the seed sector but the focus remains on the key grain crops with little attention to forages. This contrasts remarkably with other official reports e.g., the 'national livestock plans', which have stressed the importance of livestock in the economy at every level, and the need to improve productivity. This may in part reflect the separation of crops and livestock within the Ministry of Agriculture (MoA) and the research system. However, it is more challenging to make strategic interventions in livestock management, which is more socially complex than the crop sector. During the past four decades of seed sector development, there have also been political changes resulting from the federal structure of governance. These have affected the organization of the public seed enterprises and the provision of regulatory services, which are both now regional government responsibilities.In common with 'national seed projects' of the time, the World Bank project put in place the key components of a formal (organized) seed industry, similar to those found in countries with a more developed agricultural sector. These included planned production of seed with generation control starting from the breeder, and industrial-scale processing plants and comprehensive quality assurance through a certification scheme, with final marketing in sealed, labelled sacks. There was an expectation that this model would gradually expand to provide an increasing proportion of the total seed supply in the country 5 . Subsequent experience in many countries has shown that this expansion occurs in a highly selective way in those locations and crops where a financially viable business can be made. This depends on several factors including the availability of hybrid varieties, good transport networks and relatively favourable production environments.In Ethiopia there was a substantial change in 1991 when Pioneer entered into a joint venture with the Seed Corporation to multiply their hybrid maize varieties before the company separated from the corporation to become an independent subsidiary of Pioneer International. With continuing political changes, other private companies have over the years entered the market and the Ethiopian Seed Association now has 38 active members including four public seed enterprises. However, the formal seed system still accounts for only about 20% of the total seed requirement, with marked differences between crops and regions, as expected. Maize and 5. In practice the Seed Corporation mostly supplied the state farms that were prevalent at the time, rather than the smallholder sector.wheat comprise most of this seed supply with only a small amount being for teff and pulse crops. It is reported that the official system is stretched to provide full certification services and that the internal company inspections of crops are more rigorous, particularly for hybrid maize production that requires intensive supervision to ensure genetic purity.In locations where favourable conditions do not prevail, traditional arrangements such as farm-saved seed, community exchange and local markets continue as the main source of seed. These mechanisms became collectively known as the 'informal sector' because they are endogenous and unregulated but they may still meet farmers' requirements at minimum cost and with low risk, except in times of drought or conflict. For the majority of smallholder farmers in Ethiopia and many similar countries, the informal system is still the default source of seed and it is a mistake to assume it is intrinsically weak or inferior in quality.With the recognition of these parallel formal and informal channels, there came a more holistic view of seed supply on a crop-wise or national basis that is referred to as the 'seed system'. This opened an immense area of socio-economic analysis and practical research in topics such as local/community-based production, seed banks, farmer seed groups, small enterprise development and integrated approaches that link the formal and informal systems in a complementary way. Related topics such as variety evaluation and participatory breeding may also come into play if they can be accommodated within these more devolved approaches to seed production and delivery.The overall goal of all these initiatives is to improve the supply of good-quality seed in ways that are sustainable and do not depend on costly external resources. However, in all seed systems, quality assurance is a critical issue because farmers must have confidence in the purchases they make. An official label may provide that but only if the supervision of crops is done correctly. Likewise providing some level of quality assurance in a local system can be challenging. Farmers who save their own seed commonly use a high sowing rate as an insurance against uncertain germination. They may also give extra attention to a small part of the crop which they harvest and store separately for use as seed. These are all part of traditional coping mechanisms, often linked to social networks within the community or at local markets. In practice there is a continuum of seed system structures from entirely formal (e.g., for hybrid crops in developed markets) through semi-formal arrangements involving local traders, to completely informal seed-saving by farmers. In most crops and locations, there is a partition of the total seed requirement between these alternative channels that exist in parallel.The way in which seed systems work is strongly influenced by some biological and agronomic characteristics of the crop, for example whether it is self-or cross-pollinating, the types of varieties available and the seed size/ sowing rate. The value of the harvested crop in relation to seed sown is also an important factor; for example, hybrid vegetable seeds are expensive but the end products often have a high market value and farmers can make a direct calculation of the benefit they obtain from seed purchase. The analysis of seed systems is still mostly applied to cereal and legume crops in which there is a substantial annual demand, while more specialized crops such as forages are given less consideration. The 'Strategy for Seed Sector Development 2013-2018' 6 , mentioned forages only twice in its 142 pages, and then only in a general way. Likewise the document 'Transforming the Ethiopian Seed Sector -Issues and Strategies' published by MoA in 2019 made only one passing reference to forage crops in 44 pages. We may conclude that forages are still not mainstreamed in seed sector discussions, despite the importance of livestock in Ethiopia's economy.6. http://www.ata.gov.et/download/seed-system-development 3 The forage seed system in EthiopiaThe local consultant began work in mid-September 2022 and his program of visits and meetings is shown in Annex 1. The international consultant made two visits to Ethiopia in October 2022 and early February 2023, on both occasions participating in visits and meetings, with particular reference to quality assurance. In practice, most meetings provided information on both the seed supply system and quality assurance because these topics are closely interwoven.The survey was conducted in accordance with ILRI guidelines and with appropriate safeguards. It used a formal questionnaire (key informant interviews [KII]) with a standard interview guide followed by informal discussion. In addition to targeting known actors in the forage seed sector, a snowball approach was used to expand the survey. Thirty interviews were conducted across four regions (Amhara, Oromia, Southern Nations, Nationalities, and Peoples' Region [SNNPR] and Sidama) and comprising seed producers and sellers (private companies), buyers (MoA, regional BoAs, NGOs and development projects) and research centres. It was necessary to be discreet with the commercial information gathered and it was often difficult to capture company people for a private discussion. However, we believe that the survey has provided a good understanding of how the forage seed system works and it has raised many issues that are discussed later in this report. Against the background presented in Section 2 and information collected from the survey, this section describes the current status of the forage seed system in Ethiopia. Although many seed crops were observed, the survey did not attempt to investigate production techniques, only the downstream channels of seed movement.There is very little (if any) formal certification of forage seeds and there is a very clear separation of formal and informal sectors by the Ministry (explained in Section 5.3) so by definition, all forage seed production is 'informal'. However, this is a simplification because there is planned and managed production of many crops in substantial quantities by several private entities, moreover the system is organized, not casual or opportunistic. Field inspection is done by the companies and they also arrange seed sampling and testing, for example by research stations such as Holetta or the University of Hawassa. Based on the test results, labels are attached to sacks stating purity and germination. These are not 'official labels' but they do provide the essential information required by buyers. This may be regarded as a semi-formal system, even if it is not officially recognized as such. Some companies like Eden Field have simple laboratory facilities to test germination and purity and others are in the process of establishing such facilities. Key players in this trade include companies like Anatoli, Eden Field and Simeon Sitotaw that are members of the Seed Producers Association (see Annex 2), while others are not in the association, for example Bridge (Hawassa) and Esuyawkal (Bahir Dar). In addition to a local trading license, some companies have an importing license, which would be required for seeds of vegetables and alfalfa, and for some other specialized inputs. Registered seed companies are required to hold a Certificate of Competence issued by the relevant authority, federal or regional. The companies have field staff who monitor the crops, often in collaboration with local development agents (DAs), so this is effectively a contract growing system although the exact relationship between company and grower may vary. Parent seed is supplied by the company free of charge and they may obtain that from a research station or by maintaining a stock themselves in subsequent multiplications.Another element of the production system involves the use of 'collectors' who know farmers that grow specific crops for seed in different regions. They act as agents for companies, arranging to collect and consolidate seed and then supply to the company. This system was not investigated in detail and the level of supervision will surely vary. In principle, it enables farmers in favourable locations and with the necessary expertise to engage in seed production, which is a normal and positive feature of commercial contracts. However, it is a less controlled system and if seeds from different collections and locations are bulked, then traceability is lost and there is scope for malpractice. This system is also used for tree crops where growers may have natural stands or plantations from which they regularly harvest seed. Some forage seed producers are also in the tree seed business and it may be that the same approach has been carried over.The regional bureaus of agriculture also undertake some production by providing seed (through DAs) to farmers who grow a crop, harvest the seed, retain part for themselves and return part to the bureau, who repeat the process with other farmers in the following year. As a bonus, the farmers also retain the dried forage material after harvest for their own use. This can therefore be regarded as a rolling program of production combined with extension but there is no commercial transaction involved because the bureaus are not mandated to do that. In Amhara, this work is handled by a Livestock and Fishery Development Agency but the mode of operation is similar in other regions. The extent of quality assurance in this system will depend on the diligence of the DAs in the field and the technical resources of bureaus, which we believe are limited.This is the most problematic part of the seed chain because there is no conventional market-driven system involving wholesalers and retailers. There are several reasons for this; one is the long history of free seed distribution by the bureaus and projects, which has suppressed the emergence of a real market. Another is the highly administered (and deeply embedded) delivery system in which demand is collected by woreda DAs and seed is then allocated according to need. This may be appropriate for the main cereal crops to ensure equity in distribution but it is too unresponsive to enable small quantities of specific forage seeds to be delivered to widely scattered users 7 . So even if farmers were prepared to buy seed at a market price, there is little opportunity to find the preferred crop/variety on sale in a small package at a local sales outlet. ('Availability creates Demand' was a founding principle of the Kenya Seed Company when it began trading over 50 years ago!). Equally, suppliers of agro-inputs (agro-dealers) will be reluctant to stock a perishable product like seed with uncertain demand. This is a fundamental conundrum of the supply chain that must be addressed if a commercial market for forage seeds is to emerge.7. It is interesting to note that a 'Direct Seed Marketing' initiative was promoted by the International Food Policy Research Institute (IFPRI) to address this bureaucratic impediment to seed delivery. In most countries, this would be the normal situation, not an innovation.There is a widespread belief that commercial dairy production will be the main driver for growing cultivated forages and thus for seed demand. There are many commercial dairy units in urban and peri-urban areas and they have a consistent requirement for high-quality feed to maintain milk productivity. The SNV Building Rural Income through inclusive Dairy Business Growth in Ethiopia (BRIDGE) project has been involved in this sector for some years and it has clear evidence of the milk gains that can be achieved from stall-fed crossbred cows fed with high-quality forage. The project has therefore promoted forage seed sales using a voucher system that reduces the purchase price by 50% to stimulate the development of a commercial market. The seed is obtained from known producers who package it into smaller units and then distribute to agro-dealers in target woredas where the project operates in Amhara, Oromia and Sidama regions. These dealers sell at half of the commercial price (including their own operating costs) and recoup the other half from SNV through the voucher. This is a serious effort to promote milk production and forage cultivation but at present it does still require significant support from the project to maintain the system, although the value of the voucher will be reduced progressively. Beef-fattening units are not major users of fresh forage because that is a highly seasonal business linked to public holidays and it relies more on concentrate feeds that deliver more protein.Finally, we may consider how the production companies actually sell their seed. Apart from the special case of SNV BRIDGE, most of the seed is offered in bulk in response to tenders issued by regional BoAs or development agencies/projects. NGOs may follow the same procedure or they may offer a direct purchase contract to a trusted producer. The bidder is required to provide a declaration of seed quality from an independent source, such a research station or a university, as noted in Section 4.3.The precise details of the tender process may vary between the different agencies but we understand that after accepting a bid from a company, the buyer normally sends a 'task force' to check that the offered quantity of seed is available and take a sample for testing to confirm the quality standard stated in the bid. This is exactly the procedure that would be expected but it still raises the question of where reliable test results can be obtained, considering the lack of dedicated facilities and technical skills for this task.It may be noted that regional BoAs are major buyers of seed while a separate department of the bureau has the regulatory responsibility for enforcing seed quality standards. Despite this, they still expect an independent statement of seed quality. This is hard to understand; either they have no in-house testing capability, or they do not have confidence in the results.We have not obtained estimates of the total volume of seed of the main crops or of the exact routes to users but clearly it is almost all sold in bulk through tender or purchase to a limited number of institutional players who pass it on to client farmers free of charge or at highly subsidized prices. We understand that projects of the World Bank, the United States Agency for International Development (USAID) and the Food and Agriculture Organization of the United Nations (FAO) are engaged in this form of distribution, besides many NGOs. Apart from SNV, other NGOs mentioned in discussions include Plan International and World Vision but we may assume that most have been involved in forage seed supply at different times, depending on their project activities and available funds. Moreover, most of this seed finally reaches farmers through the BoAs and their DAs as part of their extension activities.To summarize the key elements of this system:• primary production is by companies using contract growers and by farmers who supply local collectors; some producers also use their own land,• production companies 'sell' most of their seed by bidding for public tenders,• buyers who invite tenders are mostly financed by external agencies, and• end users (farmers) receive seed free of charge from projects or bureaus through local DAs, or directly through NGOs.4 Discussion of issues affecting the forage seed systemIt is a fundamental principle of plant breeding that breeders are responsible for maintaining their varieties in the original state and making elite seed available for multiplication. In some situations, this responsibility may be transferred or delegated to another party, but this should still be under the breeder's oversight. In practice the details of these arrangements vary but there must still be a defined point of reference for the variety, and ideally a detailed description, so that it does not deviate genetically from the original.Certification schemes are based on a supply of genetically pure 'breeder seed' that is multiplied through named generations for as long as the variety is in demand from farmers. This is an essential element of the formal seed system. The final generation sold to farmers as certified seed is not used for further multiplication, although farmers may do this themselves and varieties may then circulate within the informal seed system. The supply of early-generation seed (EGS) has become a major issue of concern in many national seed programs/systems in recent years. This reflects the intrinsic weakness of variety maintenance and seed production in public sector breeding programs that have limited resources and do not depend on revenue from downstream sales. This is in marked contrast to private sector breeding where market share is the critical measure of success and the source of income from sales that in turn support the breeding program.The only sources of elite forage material are the research stations of the Ethiopian Institute of Agricultural Research (EIAR) and regional bureaus. The 'Technical Multiplication Department' in EIAR headquarters is responsible for this work at headquarters but it is not known to what extent they engage with, or actively supervise, the regional research stations where most of the work is done. Discussions with seed producers during the study suggest that these key linkages are weak and certainly not systematic. Seed producers report that they received an original stock from research stations but generally do subsequent maintenance themselves so there is no official control of varietal purity in subsequent generations. This procedure is not necessarily faulty but it does mean that the system is uncontrolled and varieties may become contaminated. Much will depend on the level of management by producers, particularly through inspection of their seed crops. Procedures for maintaining the 'nucleus stock' of varieties at research stations and releasing elite material, were not investigated but this is a critical link in the seed chain and should be done in the best possible way.This is closely linked to the issue of maintenance and multiplication already discussed but it has additional implications for marketing. The key reference point is the 'crop variety register' published annually by the MoA (now by the Agricultural Authority) based on recommendations of the independent National Variety Release Committee. The most recent edition of the Register (#25) was published in June 2022 and is based on results from the cropping year 2020/21 8 . This is a substantial document of over 400 pages and in each edition, new varieties are added. Currently there are 82 varieties of 26 species in the section on forage and pasture crops, mostly coming from EIAR or regional research stations. When first added to the register, agronomic information about the variety is included while in subsequent years, only the year of introduction and the breeder/maintainer are noted. The summary list of species and varieties is attached as Annex 4. We understand that some of the varieties listed in the register are now obsolete and no longer available.The conventional requirements for registration are that the variety is Distinct, Uniform and Stable (DUS) and has Value for Cultivation and Use (VCU), i.e. superior in some way to other registered varieties. VCU assessment is inherently more complex for forages than for cereal/grain crops because of the different means of harvest and utilization of the harvested material. We do not know if these criteria are applied by the Variety Release Committee in Ethiopia or how accurate the system is in practice. It is possible that registration is largely based on information submitted by the breeder and evaluated by the committee, rather than by independent trials, which would be very costly. There is a network of 13 variety testing stations at which agronomic trials can be carried out, although these will handle the whole range of crops listed in the register.The definition of each variety through a DUS examination is of special importance when there are intensive breeding programs and many similar varieties, as in some cereal crops; this detailed description is also the basis for plant variety protection (breeders rights). While the forage seed sector is not at this stage of development, having a good morphological description with key identifying characters is necessary for the inspection of seed crops to ensure their identity and purity during subsequent multiplication. This would assist companies to do crop inspections and stimulate greater awareness of the varieties that are in circulation.Discussions with producers and users indicate that there is little awareness of varieties within the multiplication and marketing system; crops growing and seeds supplied are normally identified only by species. This confirms the results of a survey of user preferences carried out by the Forage Seed Consortium in 2020 and it has serious implications. Not only is variety identity soon lost once in the multiplication system but (more serious) the genetic improvements made by breeders are not delivered securely to benefit the end users. This calls into question the value of the variety testing and release system for forages. In cereal crops the varieties are more clearly identified during multiplication, especially if certification is done correctly. Cereal farmers may recognize the varieties in common use and may have their preferences based on experience or local information. This knowledge and understanding is absent for forage varieties so farmers may not exploit the full potential of the material they receive.Given the diverse agro-ecology of Ethiopia, it may be that varieties are mostly released into the localities for which they are best adapted but this may not be maintained during subsequent multiplications in the hands of producers. Likewise, it is possible that breeders only multiply and release their most recent variety, which should gradually replace any predecessors, although in a random way. These are all potential weaknesses in the forage seed system and they should be addressed.Because the multiplication system is informal, there are no official tests of quality and no official labelling of containers. The capacity of the official seed testing stations in forage seeds is doubtful because the certification procedures have not been implemented, although in principle a system exists for all forage crops. No visits 8. This edition is attributed to the new Ethiopian Agricultural Authority and specifically to the Plant Variety Release, Protection and Seed Quality Control Directorate; these functions previously belonged to a Department of the Ministry of Agriculture.were made to the seed testing facilities of regional bureaus; we were told there are 45 in total and they are responsible for both crop inspections and seed testing under the certification scheme 9 . However, both producers and buyers do need to know the purity and germination of their material so they arrange for these tests to be carried out in whatever way is convenient; Holetta and Debrezeyit Agricultural Research Institute-EIAR stations and Hawassa University were specifically mentioned as providers of this service. We understand that this testing service is free of charge despite the limited resources of the research stations. It is an informal arrangement between the parties.These testing facilities have been improvised to assist producers rather than by having a properly equipped seed testing laboratory. Moreover, some forage species (notably the perennial grasses) are more demanding in their test procedures than grain crops so staff would need training to do this work properly and in accordance with the 'rules' published by the International Seed Testing Association (ISTA). The ILRI germplasm unit has this expertise and facilities and has provided such training as part of courses on forage seed production. We understand that there is no functioning 'central seed testing laboratory' at present within the Federal Agricultural Authority that can provide a benchmark for quality assessment and, if this is the case, it is a weakness in the system.It would seem that there is virtually no official regulation of the forage seed sector at present, neither in production nor marketing. There are some peripheral elements in the overall framework but they do not affect the routine trade, for example;• The crop variety register lists the varieties that can be marketed, but this information is not reflected in the labelling of seed containers.• Companies operating in the trade are required to have a 'Certificate of Competence' issued by the bureau or MoA on the basis of an inspection visit but this may be more a general assessment of their facilities than of detailed management procedures.It is frequently asserted that the distribution of free seed to farmers has hampered the emergence of a true pricebased market. This is a logical conclusion but it is only correct if the seed distributions occur in locations and to farmers who are producing cut forage to meet a regular demand from dairy units, thus in competition with the 'BRIDGE project strategy' for developing the market. An alternative proposition is that seed is distributed to selected farmers in the target areas of projects as a justifiable and beneficial intervention but without any specific expectation of follow up or impact. Beneficiaries would obviously use the harvested crop, they might save or share some seed and neighbours might observe the benefits -thus it is a low-level extension activity but not necessarily undermining a sustained forage demand from dairy units. It would be interesting to know the justification and expected benefits of free seed distribution in the project documents of donors who provide funds for this activity.We do not know the extent to which existing dairy units buy cut forage from farmers in the vicinity or whether farmers who grow forages are able to sell their crop to dairy units, as in a 'hay market'? There is probably a limited local trade in cut green forage but this was not investigated. The availability of teff straw with a reasonable feed value may have some negative impact on the development of a fresh forage market in teff-growing areas.It would also be interesting to know whether there is support or encouragement from projects or DAs for beneficiary farmers to maintain/distribute seed after the initial allocation they receive. If so, one might expect these forage crops to be more widely known by now and with a significant level of availability and adoption through informal channels, as happens in cereal crops. It would seem that farm-saved seed and community exchange are still very limited in forage crops, with the possible exception of oats. In cereal crops these informal mechanisms provide most of the seed used by farmers.The technical challenges of forage seed production vary widely across the range of crops. Oats is the easiest to produce, being a conventional erect cereal that can be mechanically harvested and threshed, so any company handling wheat could also offer oats in its portfolio, but the profitability is low. Legumes such as lablab and vetch require more care because of their less manageable growth habit and sequential seed ripening while perennial grasses with small seeds need more experience to determine the optimum harvest time, but they can be mechanically harvested with the right equipment. These factors all affect the scope for recruiting new producers and increasing the total seed supply. Teff is the 'benchmark crop' for good farmers because it commands a high market price but it also needs more intensive land preparation. Teff straw also has a significant cash value and this may further complicate comparison with the profitability of cultivated forage crops.Discussions during our visits indicate that vetch seed production on contract could rival teff but only if the farmer receives good advice or has gained experience from successive seasons, as for all specialized seed crops. Conversely, farmers who receive free or subsidized seed will derive initial benefit from the crop but they may not have the confidence or motivation to continue with informal production or local sales in subsequent years. Moreover, they may need assurance that a market for forage exists before they decide to switch from a more conventional crop. To summarize, forage/seed production is not a game-changer for the average farmer, it is more likely to be an interesting addition for some innovative farmers.One positive aspect of perennial forage crops is that the harvested material can provide both useable hay as well as seed and this is a justification for integrating seed production with a livestock operation. These are opportunities that alert business people can exploit to their advantage, but each case is different 10 . If the demand were to increase, then mechanization of harvesting could have a substantial impact on the cost of seed. A large crop of Rhodes grass seen near Hawassa was being mechanically harvested and this would give the grower a significant cost saving/price advantage. Likewise, the very large stock of Panicum observed in a store in Hawassa must surely have been mechanically harvested. Since forage seed distribution/extension has been practiced by different agencies and projects for many years, and perhaps also by ILRI at times, there has been considerable exposure of these crops to farming communities. If the financial benefits of seed or fresh forage production were clear, one would expect a wider adoption by now.Discussions about increasing the use of cultivated forages routinely cite complaints about the availability and quality of seed. Weaknesses in quality assurance are clear because of the lack of adequate seed testing facilities and limited traceability of seed lots in the production system, especially that which comes from 10. In many countries, it is common practice for forage seed producers to graze or cut the crop for part of the season before harvesting the seed and this is factored into the overall budget for the crop.collectors. However, the question of quantity is less clear. There has been ample research on seed production methodology by ILRI and EIAR extending over many years and a conference on this topic was held in 2011, from which substantial proceedings were published 11 . Given that several large-scale producers have entered the market and are investing in facilities, we conclude that primary production is not a constraint and if demand is there, seed will be produced. In fact, demand is there from the tendering system but it creates an artificial market because the tenders depend on external funding that varies from year to year, making planning difficult for producers.Forage seed moves widely within the country because locations of production and utilization may be far apart and some areas may be especially suited to producing particular crops. This may also encourage farmers to develop and share expertise, leading to pockets of production in favourable locations. This is a normal feature of the seed trade and in vegetable crops it operates at a global level with some remarkable concentrations of production in a few locations. Ethiopia is characterized by its very diverse agro-ecologies and this presents opportunities where seed production of specific forage crops could become concentrated to the benefit of farmers in those areas, but there should be a means of supervision and inspection to maintain standards. There are no regulatory barriers to this movement at present, and the same should apply in any future system that is developed.One potential risk in this system is that 'collectors' in distant locations may acquire seed of a particular species and send it to companies who consolidate into bulk lots for the tendering process but without sufficient knowledge of its genetic origin or adaptation. This may then be distributed under the species name to locations for which the material is quite unsuitable, for example moving between lowland and highland areas. There are reports that this does happen so that tender bids can be fulfilled and it emphasizes again the importance of more accurate labelling of seed lots in the supply chain.We believe weak market linkages are a major problem because at present there is little direct connection between the producers and end users. Consequently, it is difficult for users to access the seed they need in a reliable way and producers are less confident because they do not experience a consistent market demand. The tendering system is a major complication because it creates a barrier between producers and users, although it does provide a convenient outlet for bulk seed. In the short-term, producers do not object to free seed distribution because they have a convenient outlet for their seed, but in the long-term they would benefit from better market linkages. Some have taken steps to achieve this and NGOs would be natural partners in this endeavour, rather than large agencies or government projects.The BRIDGE project is making a serious attempt to overcome these obstacles but it still provides coordination and finance support to maintain the system for its client agro-dealers and dairy units. The medium-term plan is to gradually reduce the voucher contribution so that buyers pay a larger share of the sale price but their efforts are still challenged if there is parallel free seed distribution by development agencies within the same area. We hope this does not happen in practice. The size of seed packages is a key factor for marketing because farmers must be able to purchase a quantity that corresponds with their sowing requirement. Moreover, the pricing of such packages might be problematic -what is the market price in the absence of a real market? The price used by BRIDGE as a basis for their voucher system would be one starting point but even that is based on a price offered by the producers for a bulk order, rather than from a competitive market.Another factor relevant to marketing is the relative weakness of the private sector in Ethiopia. This is an endemic problem reflecting a long history of government intervention and bureaucratic management that has discouraged entrepreneurism. Even in recent times the Agricultural Transformation Institute (ATI), established as a policy think tank, has become an input supply organization with retail outlets. The National Seed Association has over 30 members, (including four public enterprises) but they focus mostly on a few profitable crops, especially hybrids and vegetables, and none engage with forage seeds to a significant extent.It would be interesting to try some test marketing of small packages through other outlets, for example the ATI 'one-stop shops', in areas where there is a concentration of dairy cattle. This would need to be coordinated with the BRIDGE project to avoid local conflict and perhaps with external support for 'de-risking' those involved. BRIDGE reported that some farmers who are not within their voucher scheme have made full price purchases from local agro-dealers who have stocks available. This would indicate that there is latent demand, at least in the more productive areas.The announcement of open tenders is a standard mechanism used by governments to procure goods. The purpose is to secure the best value and to promote competition between bidders but in practice this can be compromised if confidentiality is not maintained between the various parties to the process. Purchasing seeds by tender has particular risks because of the key aspect of quality, which can vary between every batch that is offered. It should be supported by a reliable system of quality testing. In the case of forage seeds in Ethiopia, tendering is convenient for producers but it has negative impacts on the way the market works, and these may be summarized as follows:• There is no direct connection between producers and users that would allow normal trading linkages to evolve; likewise producers cannot easily develop a reputation for quality or service because the identity of their products is lost in the administered distribution system through which seed reaches farmers.• There is no real market price based on actual production costs and demand; the prices offered by bidders are by definition, confidential, unless they collaborate unofficially prior to submitting bids,• Because the funding for most purchases comes through donors to regional bureaus or directly within NGOs, there is a risk of price inflation to artificial levels, making the seed appear expensive, if it were to be sold at that price.• Unpredictable funding by donors means that the annual requirement for seed in tenders varies; this makes planning difficult for producers and increases the risk of them holding carry-over stocks, with possible loss of quality in storage.• Buyers tend to accept the lowest tender price in order to purchase as much seed as possible with the funds available, thus making quality a lower priority; this effect may be compounded if the buyers are not technically aware of quality issues or if facilities for testing are limited.• From a trade perspective, the tendering system is intrinsically divisive because it makes the bidders into 'blind competitors', although in practice there is surely some conversation between them. This oligopoly market, with only a few traders involved, can lead to reduced competition and increases in price.Seed importation is a separate issue but it deserves mention because we got some information about it from the study. The only forage seed that is normally imported is alfalfa -mostly from Italy, but it could surely come from other countries. It is fully EU-certified seed that has been treated or coated so is very expensive and beyond the reach of ordinary farmers but apparently supplied to some NGOs, raising concerns about sustainability. The price was said to be 10 times that of 'local alfalfa', which is in fact Melilotus albus (officinalis?), an annual crop of much lower nutritional value. We understand that this species was promoted by some projects in the past and this caused confusion in the market because the seed is very similar to true alfalfa in appearance, although it does have value as a honey plant for bees.Eden Field is working with the Feed the Future Resilience in Pastoral Areas (RIPA) project in the SNNPR in collaboration with GOAL, an international NGO. The target of the project is mainly lowland areas where commercial seed availability is low but ample land is available and also irrigation water from rivers. Primary and secondary out-growers are producing forage seed through pre-financing arrangements and the provision of initial seed from Eden Field. Finally, the company buys seed from these growers and they use the forage material for their livestock.5 Options for quality assurance of forage seedsThis is the standard approach to seed quality assurance in the formal system; it is a comprehensive process which involves monitoring every stage of the crop from field selection through crop inspection to laboratory testing and the official labelling of seed containers prior to sale. Besides the technical operations, there must be a parallel system of record-keeping that provides traceability based on reference numbers that extend through (typically) 3-5 named generations. Thus, in principle, any certified seed that is sold in a labelled container can be traced back to a small batch of elite seed released by the breeder. Although widely adopted in countries with a highly developed agriculture sector, certification is demanding in terms of resources and it has limitations in countries with a more traditional agriculture. Even when certification is implemented in principle, there are often deficiencies in practice. Certification is compulsory for field crops in the EU but the work is usually devolved by government to an agency and payments are made at each stage of the process so the costs are recovered and ultimately passed on to farmers in the seed price. For information, a summary of certification procedures is shown in Annex 3.Certification standards have been prepared by the Ethiopian Standards Institute for a very wide range of crops; they are remarkably comprehensive, particularly with regard to seed health requirements, and they are probably based on a synthesis from other countries. In practice, these standards cannot be implemented by the seed testing laboratories and are therefore not enforced for the majority of crops grown in Ethiopia, and certainly not for minor crops like forages. This was the conclusion of the study carried out by ILRI in 2019 and it prompted the discussion about QDS as a simpler alternative.Recognizing the problems outlined above, FAO prepared QDS as a lighter-quality assurance system in which most of the technical work is transferred to the producer, but with monitoring by the official system to maintain standards. The quality standards for QDS may be the same as for certification but in practice countries can interpret this system according to their needs and resources. The key quality attributes that buyers need to know when they purchase are germination and physical purity, and even the latter is not so critical provided it is reasonably high. Moisture content may also be a concern but this varies according to relative humidity in the atmosphere so is not a stable quality attribute, unless sealed containers are used. If moisture content is high during storage, then germination will decline rapidly and producers would normally avoid that situation by drying seed thoroughly after harvest and maintaining good store management.The other important attribute of seed is the variety, both its identity and its purity. This cannot be easily assessed in a laboratory and physical 'grow-out tests' are required; these are a standard (but costly) feature of certification schemes. One of the pillars of the FAO QDS scheme is that the variety is known and registered in a 'national list'.In the case of Ethiopia, this is the crop variety register, already described in Section 4.2.Some years ago, there was an initiative by the MoA to promote farmer-based seed production for the major grain crops in woredas where the formal system was not working well. To provide a minimum level of quality assurance, a task force was set up to prepare a quality declared seed scheme and this was published as Directive No 1 in 2015.It envisaged a clear and separate role for QDS with production mostly linked to cooperatives and supplying only within the selected woredas, all overseen by a technical committee of the regional bureau. We understand that QDS has been adopted (for cereals) in Amhara and SNNPR but not in Oromia. A key concern of the ministry was that there should be no overlap or confusion between certified seed and QDS, hence its movement is normally restricted to individual woredas. It is essentially a local system, with production usually being done by designated cooperatives, although movement between woredas can be authorized if necessary.The practical details of QDS implementation, and the extent of its use, have not been investigated but key issues of interest would include:• the ability of producers to carry out their own quality assurance tests,• the information available to producers for carrying out crop inspections,• the level of monitoring by official staff (recommended to 10%,), and• who produces and allocates the labels that are attached to sacks? A distinct QDS label is specified in the directive.These tasks might all be challenging if the official system is under pressure to maintain its routine commitments to certification of cereals in the more accessible woredas.The question of how QDS might be adapted to meet the needs of forage seed producers in Ethiopia and thus improve supply, is central to this study. The current scheme as set out in the Directive No 1 is not fit for that purpose because it was conceived with a different objective. For reasons already explained in Section 4.8, it is essential that forage seeds moves freely within and between regions so that locations of optimal production and widely distributed users can be easily connected through a functioning marketing chain. Another major obstacle is that registered seed companies (i.e. the formal sector) are not allowed to engage in QDS, whereas FSPMA members and other major producers would certainly require this flexibility to develop their market.There has been much discussion about policy and regulatory arrangements for seeds, both within the ministry and among outsiders who wish to assist. We understand that a new seed policy has been sent to the minister and endorsed in principle, but is not formally approved by the Parliament. More important, there is a new Seed Proclamation (1288/2023). This has been under discussion for several years and was approved by the Parliament in May, thus becoming law. However, it cannot be implemented until the subsidiary regulations have been prepared, scrutinized by the Ministry of Justice and approved by the Council of Ministers. This is now in progress but it may take some time to complete. The new proclamation includes some fundamental changes, for example by allowing companies to self-certify their crops. This is a positive step in principle (being closer to the concept of QDS and it would also reduce pressure on the official system. We assume that the provision for QDS will continue as in the current directive or until a new directive is issued.Another major change in the regulatory arrangements was the establishment of the Ethiopian Agricultural Authority (EAA) in 2022 under Regulation 509/22. This has transferred responsibility for regulatory matters in all agricultural sectors out of the ministry, which now handles operational matters such as extension services provision. Consequently, any proposal for a QDS scheme for forage seeds would have to be discussed with and reviewed by the EAA in consultation with the ministry. Since this would have the status of a directive, we understand that it can be approved at the ministry level.6 Conclusions and recommendations 6.1 Overview of the forage seed supply systemThis study has provided many insights into how the forage seed system in Ethiopia is organized and in particular the key role of institutional buyers such as the regional bureaus, NGOs and other projects. These largely replace the conventional wholesale and retail players in a seed supply chain. This gap may be attributed in part to the long history of government involvement in cereal seed supply/allocation and it was the motivation for the 'Direct Seed Marketing' initiative supported by IFPRI. However, it is more challenging to market small units of a specialized product like forages directly to users.Weak market linkages remain a serious problem and primary producers cannot be expected to carry their goods through to the point of retail sale. We need to identify some more players who can fill this space and make the marketing chain work in a more efficient and connected way. We also need to know the real pricing structure along this chain that rewards each player according to the work they do. This is essential knowledge for staple commercial crops like cereals but it is more difficult to determine for forages where there is no grain price for comparison, except in oats.There is little direct sale by producers to farmers, except on a local basis from retail shops where sacks of the products are available, and probably some advice as well. However, there are examples of direct linkages between seed producers and forage users linked to NGO projects.Recommendation: Alternative models of seed supply that link seed producers directly to forage users at reduced cost should be documented and replicated if possible. It would also be helpful to know more about the production costs of forage seed and existing price reference points as a basis for a market-based pricing system so that farmers would know the real cost and value of these seeds.In the absence of a certification scheme, there is no formal channel through which breeders can release their varieties for further multiplication. There is a weakness at the point of transition from research to the seed system and breeders may have regarded extension/demonstration plots as the best alternative. However, there are now several companies taking seed from research stations and doing organized seed production. The introduction of a QDS scheme would fill this gap in the supply chain without imposing the demands of full certification.A further consequence of this disconnection is that the identity and purity of varieties within the production system is uncertain and difficult to monitor. As a result, genetic improvements made by breeders are not delivered to users in a secure way. The current QDS directive designed for cereals assumes that certified seed is the starting point for multiplication but this does not work for forage crops, where no certified seed is available at present.Recommendations: This is a weak link in the seed chain and a concerted effort should be made to formalize and strengthen the transfer of pure seed stocks from the breeders to companies and crucially with a variety name that should be retained during subsequent multiplication. This process could be prescribed in a protocol or a standard operating procedure agreed by all parties.To increase variety awareness, it would also be helpful to have a convenient source of information in the local languages about the main forage varieties that are in circulation. This could be based initially on the information published in the crop variety register at the time of first release and enhanced with subsequent experience from users. This would make the register accessible to a wider community. It would also be useful to carry out a definitive trial of all registered varieties in the main forage crops, some of which may now be obsolete and no longer available.We still do not know the quality of seed in the system or the reliability of the tests that are carried out, even though these are the basis for substantial transactions. In fact, the forage seed currently available is 'quality-declared' but without routine verification or monitoring by an independent agency.Recommendation: A small number of seed testing laboratories should have the essential equipment and trained staff to do routine testing of the main forage species. In addition, samples should be taken by an independent person rather than being submitted by the owner of the seed. Improving seed testing facilities would require capital investment and that would have to come from a donor. This should be a high priority for any major investment project in the agriculture sector.We do not know the criteria for free seed distribution or its intended objectives from the perspective of the DAs, bureaus and projects, beyond being considered as a convenient way to help farmers and to use available donor funds! Seed distribution done as an extension activity by regional bureaus is probably one of the main conduits through which seed moves from research to farmers; it could lead to informal diffusion but not to systematic multiplication. However, it is less certain if the distribution of free seed is the main cause of weak demand. More likely that for most farmers, the direct monetary benefits of seed purchase are not clear, despite the environmental justifications. For traditional farmers who keep one or two cattle for diverse reasons, the benefits (for the household or the environment) cannot be monetized sufficiently to justify seed purchase. The same applies to tree planting, which is easily justifiable on environmental grounds and as a community benefit but would rarely be done at scale by individual farmers.Recommendation: It would be useful to engage with these projects and to learn more about their motivation, although there may be sensitivities about external scrutiny of their activities. There could be greater effort to ensure that seed distributed in the name of promotion/extension is multiplied and diffused more widely by the informal sector. This should improve livestock nutrition but would not strengthen the formal seed sector.Establishing a real demand-driven market for forage seed is still a challenge and would seem to depend largely on the development of dairy units where improved feeding can be directly related to increases in milk production.The SNV BRIDGE project is actively involved in this sector and is committed to developing a more commercial seed market. It is possible that availability is a constraint and that there is a latent demand from farmers who are willing to purchase seed but cannot easily do so. However, smallholder farmers will always make the food vs feed comparison and are unlikely to use their scarce resources to purchase forage seeds.Recommendation: A test marketing of small seed packages should be organized in selected areas that have a significant number of dairy units but are not within the scope of the SNV BRIDGE project. These packages should carry essential agronomic information to guide users.The use of tenders for official procurement is normal practice but it has serious disadvantages for seeds because of the critical importance of quality standards. Bidding for tenders provides a convenient channel for producers to sell their seed and for donors to allocate funds but it has prevented the establishment of a stable transparent market, based on real costs and demand. We believe that this mechanism of transaction is an equal impediment to the development of a real transparent market on the production side, just as the actual distribution of free seed to farmers is on the demand side.Recommendation: Serious consideration should be given to alternative mechanisms of procurement that would give producers a fair price and reduce the cost of seed to farmers through more direct market linkages.In discussions with the ministry staff, there was a recognition that the current QDS scheme does not work well for forage seed and they are open to proposals from the Ethiopia Forage Seed Consortium on how to address this need. Given the incompatibilities noted in Section 5.3, it will be difficult to adjust the present directive and we believe it is necessary to prepare a customized scheme for forage crops. This should have the same format as the present QDS guide including a justification as well as the technical procedures and standards for implementation. Some of the recommendations proposed in this section can be accommodated within a QDS scheme. The Ethiopian Standards Institute can support this process by preparing QDS standards for all forage crops and with achievable standards for the key quality attributes, namely physical purity and germination.It is essential that any proposed scheme facilitates seed movement throughout the country. This would become even more critical if the current trend for regional autonomy continues. For the same reason, it is essential that seed standards, as set out in the regulations, remain a federal issue and without any divergence. We understand that the EAA already has an arrangement in place to ensure the smooth movement of seed between regions, since this is essential for hybrid maize, for example.Recommendation: A draft QDS scheme for forage seeds will be prepared as an activity of the Forage Seed Consortium and all interested parties will be consulted to ensure that it meets their requirements and can be implemented. The Producers Association should play a key role in this and should be encouraged to use the scheme.The challenge of increasing the use of cultivated forage seed has been recognized for many years but has proved very difficult to overcome despite its importance. Several of the issues raised by this study have policy implications and we believe they should be discussed in a high-level meeting between stakeholders and policymakers from both the crop and livestock sectors. This was a key recommendation of the consortium meeting in February 2023 but it was also noted that there must be good preparation for such a meeting to facilitate a constructive discussion and favourable outcome. The opinions of some local seed experts were also obtained from informal discussions. Two meetings of the Forage Seed Consortium (September 2022 and February 2023) provided many further insights from stakeholders; summary reports were prepared for both meetings.Annex 3: Key elements of a seed certification scheme (and suggested importance or relevance to forage seed crops in Ethiopia) This must be checked/confirmed by the grower but it is less demanding than for cereals because of the small number of varieties in production Seed crop is inspected in the field and a report prepared; number of inspections may be specified, according to the crop This must be done by the company or its representative to confirm the identity and uniformity (purity) of the variety; a lower standard of purity may be accepted in forage cropsSample is taken from the seed lot after cleaning using approved sampling procedures Essential -correct sampling procedure must be followed to obtain a valid test result Sample is analyzed in a laboratory and report is prepared Essential -but only purity and germination are required for foragesIf test results meet minimum standards for the crop, seed lot is 'certified' and given a reference number Essential -to give the seed lot identity and reference number is shown on labels to maintain traceability All seed containers are sealed and carry an official label with required quality information and the reference number (label issue and printing should be regulated)Essential -but label must not have too much information so it can be easily printed Grow-out plot may be grown to confirm varietal purity of the seed lot This is costly and less important than for cereals crops because of the small number of varieties in use NB: All key stages must be recorded in an administrative system to provide traceability through the reference numbers given to seed crops and lots.A Quality Declared Scheme has the same elements but with simpler quality standards and with many of the practical tasks devolved to producers. QDS producers must maintain good records of crops and seed lots for inspection and monitoring purposes.Annex 4: Extract from the crop variety register, Issue No 25 (June 2022)","tokenCount":"11466"} \ No newline at end of file diff --git a/data/part_6/1216004911.json b/data/part_6/1216004911.json new file mode 100644 index 0000000000000000000000000000000000000000..825ff7b941c7c0840c36458901acd5ad9788cb60 --- /dev/null +++ b/data/part_6/1216004911.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"39ce4f9e262a787456489e8a5ef530b5","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c6694ffc-c10a-45d7-9e9e-7f4c8d9ceca9/retrieve","id":"-765599693"},"keywords":[],"sieverID":"8891b782-7123-489d-a495-fea75d2cb8cf","pagecount":"10","content":"o Carlos Lascano y Luis E. Tergas* Dos importantes decisiones que deben tomarse en una explotación ganade 6 ra con base en pasturas, es la de sistema de pastoreo y carga animal a 7 emplear. Básicamente las alternativas de sistemas de pastoreo giran alrede-S dor de un sistema continuo o alguna forma de rotación de potreros. 9Grandes discusiones se han formado en torno a los sister.1as de pastoreo como consecuencia de resultados experimentales divergentes. Es as! como 11 algunos informes señalan que el sistema de pastoreo rotacional es mejor que el continuo y otros muestran lo contrario. No es el objetivo de este trabajo dilucidar esta controversia, sino más bien hacer un análisis de algunos factores que se consideran importantes en la utilización de mezclas' gramíneas y leguminosas en el trópico, particularmente en áreas de suelos 161 ácidos e infértiles, en donde el valor de la tierra es relativamente bajo y el énfasis es el de aumentar producción por animal. Con tal fin se citan 18 y discuten algunos trabajos de la literatura en donde específicamente se h 1 . medido producción por animal y persistencia de las mezclas en términos de sistemas de pastoreo y carga animaLconsideran tres tipos de pasturas: (1) nativa ('2¡) gramíneas puras intro-2 ,ducidas y (3) gramíneas en asociación con leguminosas. Tanto en pasturas S nativas como de gramíneas puras, los sistemas de pastoreo en rotación 4 estudiados no ofrecieron ventajas sobre sistemas continuos en términos de 5 producción por animal. A igual conclusión llegaron Paladines y Leal (197B) 6 quienes reportaron que en Carimagua, Llanos de Colombia, un sistema de rotación de 4 potreros en sabana nativa produjo menores ganancias de peso 7 8 por animal que pastoreo continuo. 9La falta de respuesta en produéción por animal con sistemas de pasto-10 reo rotacional aplicados a pasturas con base de gramíneas parece deberse 11 a la eliminación en gran parte de la posibilidad de selección del animal, 12 que se ve forzado a consumir una dieta de menos calidad nutritiva '13 (Humphreys, 1967). Esto es particularmente ~ierto en el caso de gramíneas tropicales en las cuales la éalidad nutritiva cámbia considerablemente con edad y entre partes de la planta (í.e. hoja y tallo). (Lareqo y .ünson, 1973; Lascano, 1979; CIAT, 19BO).Por otro lado, la evidencia experimental en cuanto a efecto de siste-18 ma de pastoreo en asociaciones gramíneas-leguminosas es limitada e incons- 4 135 kg/an/afio) más producción por animal en el sistema alterno. Contraria-5 mente, Stobbs (1969b) encontró que un 5.7% de mayor aumento en el ciclo de 6 pastoreo de 35 días en relación a 17.5 días no fue signiricativo. En el 7 segundo trabajo de Stobbs (1969c) el pastoreo continuo y rotación en 3 po-8 treros produjo ganancias de peso similares (99 y 95 kg/an/año) pero mayores 9 que las ganancias obtenidas en la rotación de 6 potreros (84 kg/an/año). En 10 base a los resultados de los tres trabajos es difícil poder sacar una con-11 clusión general sobre el erecto de sistema de pastoreo en producción por 12 animal en asociaciones gramíneas-leguminosas. Además, es impqrtante tener 13 en cuenta que el sistema de pastoreo a emplear en una asociación gramínea-14 Leguminosa no debe estar únicamente basado en resultados de producción por 15 animal a corto plazo, sino que además debe considerarse el erecto del sis-, tema en la persistencia de las especies asociadas, lo cual está ligado con productividad de la pastura a largo plazo. Este concepto desde luego tarobién se aplicaría en el caso de pasturas a base de gramíneas en áreas donde existen problemas de malezas ( i.e. suelos fértiles y alta precipitación ).En el trabajo de Stobbs (1969c) se estudió el efecto de sistema de pastoreo 21 en la composición botánica de la pastura. Se encontré que después de 3 años de pastoreo había menos proporción (P<.05) de Panicum maximum en el trata-23 miento de pastoreo continuo que en cualquiera de los dos sistemas de rotacíán estudiados. Además se\"observó que el pastoreo continuo estuvo asociado con mayor invasión de malezas. El autor discute estos resultados en términ 26 de que en el pastoreo continuo hubo mayor posibilidad de selección que en :.:... '-.';: . , .~. 1. Irrigación y conservación de forrajes. La rotación facilitaría estas prácticas.2. Salud anim&l o animales con requerimientos especiales. Una rotació púede ayudar sn el control de parásitos internos o externos y simplificar el uso estratégico de pasturas en base a condición fisiológica de animales (i.e. vacas lactantes, secas, etc).3. Persistencia de especies más seleccionadas por el animal.El tercer punto expuesto por Humphreys (1976) es indudablemente de gran relevancia en el manejo de pasturas a base de gramíneas en áreas con problemas de maleza y en el manejo de asociaciones gramínea-leguminosa, en las que se sabe existen grandes variaciones durante el ano en la selección del animal de los componentes de la mezcla, tanto en zonas subtropica les como tropicales. Stobbs (1977) encontró que en una pastura de Setaria anceps cv. Nandi + Macroptilium at~opurpureum cv. Siratro, la selección hacia la leguminosa cambió con época del ano y con el consiguiente efecto aumentó la proporción de leguminosa de 6-9% a 37~~9%. Otros resultados ¡ 2 (Jones, 1979) muestran que una mezcla de Setaria anceps con Macroptilium 3 atropurpureum, bajo diferentes cargas y frecuencias de pastoreo, la legumi nosa disminuyó a medida que se aumentó la carga de 0,8 a 2.8 an/ha, par-5 ticularmente en la frecuencia de 3 Semanas, Sin embargo, la disminución de 6 leguminosas debido a carga fue menor en el pastoreo con frecuencia de 9 se 7 manas.Basados en la evidencia experimental revisada es difícil decidir so- nes bajo pastoreo podrta estar afectada por:1. Agresividad inherente de las especies, muy ligada al hábito de crecimiento.2. Pastoreo selectivo de las especies en función de palatabilidad relativa y época del afio.3. Manejo del pastoreo en términos tanto de sistema como ca~ga animal.El Programa de Pastos Tropicales del CIAT, a través de la Sección Productividad y ~anejo de Praderas ha diseñado una serie de ensayos de pastoreo en la estación de Carimagua, Llanos de Colombia con el objetivo de estudiar el efecto de sistema de pestoreo y carga animal en la producti vidad de asociaciones gramíneas-leguminosas contrastantes. Es así como en 1982 se iniciará el pastoreo experimental en mezclas de:1. Brachiaria decumbens -1' Desmodium ovalifolium CIAT 350 bajo pastoreo continuo y alterno cada uno con tres cargas (1.2, 1.8 Y 2.~ an/ha).2. Andropogon gayanus cv. Carimagua 1 en asociación con Desmodium ovalifolium CIAT 350 y con Pueraria phaseoloi~ en pastoreo alterno y con I 'i .i . Es bastante evidente que no existen marcados beneficios en producción 7 por animal con el uso de sistemas rotácionales de pastoreo en pasturas 8 nativas o de gramíneas puras introducidas. Es menos claro, sin embargo,. '.-10- ","tokenCount":"1129"} \ No newline at end of file diff --git a/data/part_6/1218046734.json b/data/part_6/1218046734.json new file mode 100644 index 0000000000000000000000000000000000000000..357f637e42a73a70454bf488d390fedaa3a434cd --- /dev/null +++ b/data/part_6/1218046734.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"afab41deec2f120b042ebc6404aeb7f9","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/75a36edb-2abd-4591-8164-3c1781611c14/retrieve","id":"549988402"},"keywords":[],"sieverID":"575efe3c-7784-4e35-937b-ca2b67294291","pagecount":"66","content":"Thr。υgh the cooperation of the Instituto Colombiano Agropecuari 。 (ICA). loundations were laid lor work on beel proouction systems . Gr.z ing trials were started on the north coa5t , at the Carimagua Research Center in the eastern plains (105 Ll anos) and at CIAT headquarters. ' 6 and IR22 are cJ ear when millecl and cook dry and Iluffy , demonstrating grain quality desired by Latin American consumers Despite lhe nutritional edvantages 01 high -Iy別 ne maize, problems of prcducer and consumer acceptance have been encountered. As most 01 these problcms relate to the Ilc-ury, soft cndosperm 01 the opaque-2 maize, ellorts to develop a Ilint-type maize which retains the high-Iysine characterist:c were accelerated , and preliminary results are enc。 υragmgThe cassava germ plasm collection , begun in 1969, was expanded t。 inc1 ude a total 01 2, 193 cult 川a 門, these coming from Colombia , Ecuad。人 Pυerto Rico, Panama and Peru. This col!ec'ticn , now being cfassified, will ba the basic material for the cassava production systems program , which is directed tcward an increase in the production and utilizaL(ion of improved quality cassava in the lowland tropìcsThe swine production program developed a I 刊e-cyc1e leeding system which replaces 'traditicnal commercial protein supplements wilh high-Iysine opaque.2 maize. Oepending on prevalent local mark_ et prices of these commercial pro(ein supplements, the possibility of substan'~ial savings, especia ll y for small farmers , appears feasible Individu 訓, program , and commodity work at CIAT is directed toward the development of integrated production 5γstems. Specific inputs pres. ently being developecl will eventually lorm a part 01 total larm production systems appropriate 10 the varying conditions of the lowland tropics Through sυch an orien'tation, CIAT hopes to help incorporate areas of low production within the lowland trop自 cs 川 to the mainstream of the national econom 悶 5 of l.atin America 、 'JThe year 1970 was one of basic organizational activity and imple_ mentation of research and trainir、9 projects. Seven senior staff members joined CIAT dυring the year, increasing the total number of continuing .nd term .ppointments to twenty-six. In April , CIAT he.dqu.rters w.s moved from Cali to \"EI Porvenir\" farm , where s'taff offices and housing facilities for some trainees have been temporarily located in the renovated larm buildings , pending completion 01 the perm.nent inst.11 剖 ions. Work began in June on the Lonstrυction of the fjrst permane叫t building, St.tion Operations Two organizatiuns joir、 ed thc Rockeleller, Ford and W. K. Kellogg Foundations in the oper.tion.1 lunding 01 CIAT programs during 1970 The Agency lor Internation.1 Development (USAID) contributed $ 275,000 to help finance research and training activi'ties in plant proteins and cass.va. The Interameric.n Development 8ank (81D) now helps to support CIAT training with a $ 300,000 contr.ct which provides lor 50 man-years 。 f crop and livestock production speciallst training ln Oecember, eleven graduates in animal sciences and veterinary med icine completed the 訂 rst ccurse of the livestock production specialist training project , which attempted to solidify and complement university training with direct on-the-job experience in the so)ution OT specific livestock production problems. This group 01 young men , alter completing an intensive three-month training period at CIAT headquarters, spent eight months on cattle ranches on the north coast of Colombia Results 01 breeding effor'ts with rice led to the joint decision 01 CIAT and ICA 10 n.me IR930-31 -1-18 as the variety CICA 4 lor release in early 197 1. This dwarf variety has yielded 70 per cent more th.n Bluebonnet 50, a variety popular with farmers and consumers , in trials in Colombi. . Along with CICA 4, CIAT and ICA will release seed 01 IR22. developed and named bγthe Internation.1 Rice Research Institlite (IRRI) in late 1969. While this variety was being multiplied and .dopted by tarmers in Southeast Asia , CIAT and ICA found , in accelerated tests in typic.1 lowland rice lields in Latin America , 'that I R22 yielded about 60 per cen'l more than Bluebonnet 50. The long, slender grains 01 CICA 4Several factors have influenced the development 01 a large beel industry in the tropics. There are extensive pasture lands and generally abundant leed reSQurces . Internal and external demands for beef are 5tron9. Beef cattle have Ht well in the evolυtionary develcpment of new lands and the exploitation of marginal Jands not suited for crop prodυction CIAT's Beel Production Systems pro gram is directed toward development 。f an adequate technological base and the training of action-minded produc tion personnel to support an efficient beel cattle indυstry in the tropicsWork in pastυres and forages con centrates on 'two ecological environments representative of the humid , semi humid lowland tropics of Latin America alluvial 50il grassland regions and latosclic grassland areas Alluvial 5 。自 1.Pasture vegetation in more alluvial soll areas includes introduced grass species (generally Irom Alrica) sυch as Guinea grass (Panicum maximum), Pυn1tero (Hyparrhenia rufa) , Para grass (8rachiaria m 叫 ica) and Molassses grass íM叫 inis minutiflora). Beef production in these pastures is commonly 70 to 80 kg(ha , while the potential productivity is more than 300 kgj ha . Prodυct 川 ty of the5e pastures decreases rap idly wi'th depletion 01 soil nitrogen . 圳的 the decrease in nîtrogen fertilîty , overgrazmg r-e sυIts as the grass specles lose their vigor, and the effective grazing area 1s continually decreased through weed invasion Farmers control weeds principally by hand , although in some instances they use organic weed killers which also kill the na'tive nitrogen-fixing leguminous specres Activitles in this ar~a , therefore, focus on improvement of so1l fertility though application of -àppropriate man agement in terms of stocking rate, grazing systems, and increasing soil ni trogen levels. Establishment of mixed grass-Iegume pastures and 川 troduction cl legυmes int。凹 1St 川9 grass pastures receive attention as nitrogen fertilizer app/ication on pastures used for cowcalf systems does not appear economi cal at present Coa s'tal alluvial plains such as the northern coast of C%mbia often con tain two distinct zones: (1) fertib val Icys (such as tho Sinu Valley) with a high watertable, and a dominance 01 Para grass and (2) rolling hill regions with poor quality soil5, ano a do minance 。 f Puntero grass, 5uch as in the De partme巾。 f Bolivar, where cow-::alf 。 perations predominate In cocperation with ICA, CIAT wi Jl establish a grazing tr1al at the Turipana Research Center in the S 川 u Valley to determine lhe effects of management 。 n the prcdυ c'tivitγ 。f Para grass pas-tures lor beel fattening. Three grazing systems will be used -con'tÎnu。υ5 , alternate (two-paddock rotation) , and rotati。他 1 (six-paddock rotation) . Each 01 the systems is 'to be tested under three stockìng rates Prelim 川 ary arrangements have been made to set Up trials on the ir、 trodυp tion and establ 時 hment 01 tr。戶 cal legumes in Bolivar in 1971. Grazing trials using grass-Iegume mixtures willl als。 be es'tablished in connection w 川、 CIAT' s Li vestock Prodυction Specialist Tr剖 n ing Program Fertile, high-valuo land noor population centers Near large pcpυlation centars , livε stock production is often a secondJry 。 ccupation. 8ut local demand for animal products is high , so poorer lands tend to be used lor livestock production. The Cauca Valley in Colombia , the Guayas rive'f basin in Ecuador, and 50me 01 the land surrounding Lake Maracaibo in Venezuela are examples of this type 01 enterprise 8eef production becomes more intensive through competition with other tγpes 01 larming. 1 npυts jncreδ5e to improve total animal outpu't per unit area, and nitrogen fertilization and irri gation take priority. This type 01 en terprise is directed toward growinglinishing ra'ther than bre.eding opera tions A study 01 beel production costs υnder an intensive management of Pangola (Digi 恤 ria decumbens) pastures with nitrogen fertilization and irriga tion is underway at CIAT's farm. Nitro gen levels used are 200, 4日口 , 600 and 800 kgj ha/ year_ Three stocking ra'tes are superimposed on the nitrogen treat ments in a 4 x 3 factorial des 呵n. AII treatments are irrigated according to needs Para grass (Brachiaria mutÎca) dominates ín the humid vallevs of Colombia and is widely used for finishing beef cattle. A similar trial to determine becf 8 prodυction ccsts using Para grass under intensive management with nitrogen fertilizatíon is also planned. Three levels 01 nitrogen -200, 40日, and 600 kg 'haj year -will be used. A single stocking rate will be adjusled to the product 的n 01 lorage in we't and dry seasons A trial ínìtiated in Oecember, 1969, was designed to measυre t he effect of castra'tìon, stilbestrol implantation (DES) , and inj 配叫。 n of vitamin A on lhe growth 01 grade bulls and steers A gr。υp of 284 animals was separat~d into bull and 舟er groups. Treatment in eilch group included the 101l0winQ control , 30 mg DES implantation , 400 IU vitamin A injecticn , and 30 mg DES implantation plus 400 IU vitamin A injection Partial results of this experiment are shcwn in Table 1. In general , weight gains showed an inverse relationship to ~he amount of rainfall , i.e, when rain inc:reased, iive weight gain decreased. This is possibly associated with a markocl decrease in forage intake during the rainγseascn , which in turn may be caused by the increase in moisture content of the pasture (Iow concentration 01 digestible energy and digestible pr。 tein ), as well as by the s'tress of the wet weather on the animals Over the entire perioc DES implantation markedly increased weight gains in steers but not in bulls. However, r~ sponse to DES in steers was obs. uved only during the mon'ths 01 high weight gain. DES seemed to have a negative effect in both steers and bulls dυrlng the low weight gain months. N 。悶, sponse was obtained from vitamin A injection. The experiment will continue during 1971Beel production levels are low on exis'ting native grass pastures due t。 inherent low nutritive valυe 01 these grasses and lurther accentuated by extensive systems of exploitation resulting in undergrazing , which , ln turn , !eads to grazing overly-mature pastures of low nul 鬥 tive value. Consequently, study of improved management systems for native pastures seems advisable Since grazìng ìntensjf;ca~jon Is usual-Iy associated with a change in th. z: b。 tanical composition of native pastures , low nutritive value species presently dominan't in the savannah (as a sub. climax of fire, poor soil fertility , and υndergrazing) cculd be replaced by species of higher feeding value under more intensive grazíng systems One experíment ;n Carimagua uses two grazing systems and three intens:ties of grazing to determine the effect 。f pasture rota'tion and stockìng rates 。 n the productivity of the native pastυre. Changes w hich take place in botanical composition as a result of pasture ro tation and stocking rate are observed. Continu。υ5 grazing and fourpaddock rotational grazing treatments each have stocking rates of 0.2, 0.35 , and 0 .50 animals per hectare Preliminary observations at the Carlmagυa Research Center and on private ranches in the Ll anos indicate that improv.ed grasses suc h as molasses grass (Melinis minuliflora) and puntero (Hyparrhenia rufa) can be satisfactorily established. Although establishment costs are high , particularly where fertilizer is applied , this initial investment in improved pasture can probably be quickly recovered if these pastures are used for weaners, during breeding sea son and for fattening st自 rs , and all periods during which adequate nutrient intake is highly critica l.At Carimagua a study is underway t。 determine the productivity and the economíc feasibility of growing and fjnish ing cattle on molasses grass pastures , with and without phosphate and potash fertilization and using varying grazing intensities. Fertilizer treatments used w hen establishing 'the pasture plots were: no ferti lizer; 500 kg, ha of bas 比 slag (75 kg of P, O,); and 500 kg!ha of basic slag plus 100 kg of mυriate of potash (50 kg of K). Three grazing intensities are used. The fertilizer effect has been apparent in that fertilized plots were ready for grazing 100 days after seeding, while the unfertilized plots were not ready after 300 days 抖。duction Systems Beef cattle production leveis are notorioυsly low in the Colombian Ll anos using traditional management practices Preliminary evidence indicates (h at productivity and profitabilitγcould be sυbstantiaJly increased using sound pasture management , feeding, breeding and herd health practic間, combined with some improved pasturas A series of beef catt le production herds using grade Zebus native to the area are being set up 'to test this hypothesi s. Comparisons include 1 ) traditionn l ve rsus improved cattle and pasture man agement systems, 2) na'tive grass versus native plus some improved grass Particular attention will be given t。 providing adequate nutrient intake dur ing the dry season. Primary consid. eration is identifying graSS ' 2S and gras:: legume mixtures that providc an ade quate diet through the dry season However, where available forage does not provicle aclequate nutrien't intake, attention will be given to prote 巾 , and perhaps energy supplementation Many soils 01 the lowland tropics are 01 low lertility, resulting in pa s tυre for agc deficier、 in certaln mlnera 峙,的, pecially phosphorus. Delinitive studies are needed to characterize mineraJ comØ positicn of these grasses, and consequently yearøround supplemen tal m 川 eral nee:Js In Palmi 悶 , attention will be given t 。 by-products and crop residue utiliza tion, and the USe o f cυJ'tivated forages for dry season feeding 10 ANIMAL HEALTH Tox. ic plant studies A syndrome referred to as \" vacas ca idas\" (Iallen cattle) by ranchers 01 the Icwland tropics has long been roc-O!:il nized . This disease at~acks all ages 01 cattle, bυt is more prevalent in young calves. Lack of muscular coordination .s usυa l1 y accompanied by a wheezing laryngeal sound , and when anlmals are moved any dis'tance they collapse from exhaustion . In an initial survey of the cattle-raîsíng areas of Colombia this sy ndrome was cbserved throυghout the ncrth coast area , thc Magdalcna vallev , and the Valledupar region. Insecticide into x ic剖 j o n , parasitism, vitamin E-seJe nium deficier、 cy , and various plants were mentioned as possible etiologic lactors . Common in all these areas was the pr.esence of two weeds, cansaviejo (Mascagnia concinna) and anamu (Petiveria alliacea)_ Detailed investlgatlons are being car. ried out in the new ICA-CIAT veteri. nary research laboratories in Turipana A condition similar to that observed in the lield has been produced by leeding cattle Iresh anamu at a rate 01 2 gm / kg live weigh t. In addition !to producing Disking native range jn preparation for seeding into Melinis minutiflora. a muscular dystrophy, the plant als。 appears to be toxic for the kidne' γs Studies in progress include pathogenesis of the disease syndrome, soil and plant analysis, and description 01 the disease in the field Anamu is aromatic and palatable, and grows in Ithe shade of fence rows. Animal s in the experiment appeared to ha ve a stro ng predilection for or ad-dic t ion to the plan t. This suggests that animals grazing on weedy pastures consume anamu thrcughout the year Another imp。內 ant disease 5γndrome in Colombia is \"piel caida\" (Iallen skin) . or photosensìtization. A great variety of plants produces this disease Studies are underway at Turipana t。 assay var的 us local plants lor their ability to produce this syndrome Melinis minutiflora seedlìngs three months after seeding.Crippled calf grazed on anamu-irúested pasture!: showing clinical 剖 gns of muscular djstrophy 80圳 ne enz:ootic hematuria Bovine enzootic hematuria is a tumor ccndition of the υrinary bladder whic h results in seri 。 υ s economlc losses to the ca ttle industry . In many developing zones it is the major limit. ing factor for efficíent meat and milk producticn A cooperative project has been initiated at \" La Esmeralda\" farm near CIAT headquarters where pastures have been indentified as having high , low, and intermediate incidence of hematυ ria. Plant classification sltudies and soil analyses are in progress . Bracken fern (Pteridium aq 叫 linum) is commonly found in pastures of high-disease inc 卜 clence . This plant is also associated with hematυria in other parts 01 the world Calves have been placed in each 01 the three pastures to ve rify the incìdence of hematuria. Complete urinalysis, hemograms and serum calcium j phcsphorus analyses are being per. formed 01 appr。別 mately 900 randomly selected urinary bladders Irom cattle in the Manizales area , 28 percent had gross lesions and 50 percent had microscopic lesions. Twenty-si x percent of the males and 32 percent 01 the lemales had lesions , 'thus eliminatìng sex of the animal aS . a va riable. Electron microscopy of this material is in progress Anamu (Petiv盯 la alliacia) is associa ted with a muscular distrophy of calves. This plant grows primarily in the shady areas 。( (ence rowsBovine anaplasmos時, babesiosis and t rypan osomi as is cause large production losses and mortality 01 cattle in the lowland tro pical areas of Latin America Since irradiated vaccines have been successful with o ther protozoa and me tozoa , an attempt is being made to adapt 竹、ese methods to bo圳 ne babesiosis caused , in this 臼峙, by 8abesi. bigemi na . Purilied blcod isolates c。仆 taining 1 x 10'0 organisms we re sub jected to 24,000 to 60,000 rad 01 ga mma irradiation and were inocul ated 。 nce into fou r groups of ça lves, eaçh with positive and negative irradiatecl çont rol5 , as well as he 帥 -inaçtivated organ 悶 ms and two lower dilution groups Twenty-eight days lollow ing the inocυ la tion 01 the irradiated bl∞d , all calves we re challenged with 8 . bigem ina organisms . Sυff峙 ient time has not elapsed to check the exact degree 01 immunity, bυin general immunity increased as radiat ion dosage deçreased . 11 appears, therefore, that there are poss;bilities for future use of a radiation-attenuated vacclne Premunitio n against b。圳 ne babesio-別 s has been studied using Gan aseg and the new 8urroughs-Welcome compound 4A65 . Ganaseg was administered t。 calves at levels from 0.5 to 2.5 mg/ kg e句 ht days alter in配 ulation 01 virulent 8abesia bigemina and 8abesia argentina . Result s showed that 0 .5 mg/ kg was near ly as effective as 2.5 mg/ kg in treating the early inlection . It did not ster ilize the infec'tion, thus giving excellent co-infectious immυn i ty . Compound 4A65 was us ed lor treatment 01 babes 的 S1 S 剖 dosag峙。1 0.3 and 2.0 mg/kg eight days alter inoculation 01 virulent 。 r gani s ms . At 0.3 mg/ kg the comp。 υnd was not effective for control of B. argen t ina , but did control B. bigeminaThe 2.0 mg/ kg dosage sterilized the calves, leaving them fully susceptible to reinfection Premunition against bovine anaplasmdsis wit h oxytetracycl ine and Bur. rough s-Welco me compound 356-C-61 has a lso been studied. The two drugs were υ sed separately at 5 and 12 mg/ kg, respec'livel y, to treat cattle 21 days after exposυre to the disease, with relative-Iy little success . The most successful treatment cons 叫 ed of a combined d缸 , age 01 12 mg, 'kg 01 oxytetracycline and 5 mg/ kg 0 1 356-C-61 , which was app lied 21 days after exposure to virulent Anaplas ma m argìnale within the bovine host Lesions caused by the growth and development of the protozoa in the tis ' sue5 of the ca'ttle were al50 examined These studies may reveal a weak link in the vertebrate cycle of the protozoa where the organism could be arrested without injury to the host Colonies of non-infected B. microplus ticks are being established in order t。 inlect thεm with A. marginale, B. bigemina, or B. argentina . These infected ticks will be used as a soυrce of inoculum for premun 忱的 n with treatme;1t They will also be used to study the lifecycle of the organisms in the t 時 ksVesicular 訕。 matitis (VSV) affects catt 峙 swine and horses in Latin Ame rica. I t frequently complicates r缸。gnl tion of hool-and-mouth disease (FMD) since the two dis.eases are undistinguishable in 'the fieJd. The source of virυs is still unknown. The natural history of VSV is being studied. During 1970 tw。 。 utbreaks were followed. An investiga tion of the ecology of this virus was started at a farm which usually has this disease. A master's thesis is in progress to study the role of white blood cells during VSV infection in cattle \" Vacas infladas\" \"Vacas infladas\" (inflated cows) is a condition of the pregnant bovine lemal. Th 悶 was lollowed by an eight month rn-servlce 廿 aining period on eleven private ranches in the Bolivar savannahs Following a preliminary survey conducted in December, 1969, an in -depth analysis was made of those ranches which the 間 itial sυr vey showed to have the greatest potential as collaborators with the training project , and as multipliers w 川\"\"1 in the communìty. On the eleven ranches selected a trainee was assigned to each for 30 days to give the program and the rancher opportunitγto evaluate each other, and to provide the pr。 gram with data essential to the devel 。 pment of a specif 悶 production im provement program. Nina ranches with a total area 01 10 , 5日 o hec'tares and a range in size from 170 to 4,000 hectares were included in the program. These ranches had a total 01 1 口, 500 animal units 01 cattle with a range 01 250 t。 4,000 animal units. Seven of the nine ranches were essentially owner-operated, and the othS! r two were v 時 ited once 。 r twic.e weekly by the owner Provision was made in technical assis tance contracts to pay reasonable fees for services rendered. The production improvement program , as developed in conference w 川、 each rancher, was appended as pa r't 01 the contract, as welJ as some allowance for limited extension efforts 川 the surrounding area , CIAT expects 'that these trainees will di sseminate the prac ti cal orientation t。 problem solving whi ch lormed the basis 01 their training . The multiplier effect that these trainees wil l have on key cducational and agricurturaJ assistance or ganizations within Colombia , and their ability to effect change in the orientation o f naticnal programs and the general phi losophy and app roach 01 decision-makers, will be the ultimate measure of 'the success of thìs projectIn the coming yea r, the project will strive to idenlì fy the training techniques which could lead to less costly (more trainees perυnit cost), more effective programs for tropical reg ions of the Americas To this end, ð ranch enterprise mocle l is being developed which will be used to simulate the overall functional 。peration of collaborating ranches. This wi ll provide the trainee insight and understanding 01 'the total operat ion al and 。 rganizational structure of a particular ranch (01 whlch he 悶怕 identily 18 the productio n-limiting lactors and then program the specific production improvement practices to increase productivity) AGRIC ULTURA L ECONOMICS In view of poor or non-existent statistics on most aspects of the cattle industry in Colombia, a large-sca le, on-thE• larm s'tud y was begun in 1970. The ques'tionnaire to be used has been fieldtested on the north coast. Data on calving rates, mortality rates, and growth rates in different areas 01 the country, as well 前 transport and marksava producti叭 , yìeid , and cultìvated area fo r all producing countrÎf.'s has been prep.red .t CIAT from second.ry data, partlcularly FAO publications . Production par c.pita has also been calculated lo r each country for the I.st 10 years World production 01 c.ss.v. h.s been increasing in the last fjve years. South America and Africa eac h account fo r .pproxim.tely 37 percen t of world c.ssava production , As Îa abo ut 25 percent , and Centra l America an d Oce.nia the remainder. On a per capita basis there has been a slight decre.se in worid cassava productionOther studies in progress include wholesale and retail cassava prices in 14 Colombìan cìtìes over the p.st 10 years , ∞ nsumer demand for cassava , cass.v. flour and st.rch in the Ur川ed 5tates durin日 the I.st 14 ye.rs; a descnpt 的n and analys 悶。f cassava starchproducing f.cilities in Colomb咱; the marl吋ting of cassava and cassava starch ìn Colombia; and an analysis of eco. nomic factors related to low yields in Colombia V -Cassava utl llzation through swine feeding CIAT's swine program contributes t。 'the increase of pork production in the lowland tropics through collaboration with national agencies in the development of production systems based On utilization principally of non-gra 川 leed stuffs available in the tropics, and throυgh trainin9 swine production and research specìalists for national institu tions.The use of opaque.2 maize to replace normal maize greatly reduces the quan tity 01 supplemental protein tha't is r-oquired for swine production. Research completed during 1970 demonstrated that a 12 percent protein diet based on 。 paqu e-2 maize plus a protein s upple卜 ment can replace a 16 percent protein diet based on normal maize plus a protein supplemen't lor 仰伊 during the growing period Irom 10 to 50 kilograms weigh t. During the finishing period (5日 t。中 o kilos). supplemental protein can be completely eliminated when opaque-2 maize ís used to replace a 12 percen't proteín normal maize-protein supplement diet A variety of supplements can provide the additional protein in opaque-2 maize diets for growing pigs . Optimal results have been ob'tained wi th soy bean meal , fish meal , and meat meal , 。 r combinations of fish and meat meal with cottonseed mea l. Less favorable results have been ob tained when cot tonseed meal or a combínation of cot tonseed and soybean meal ís used , demonstrating that 'the level 01 Iys ine in cottonseed meal 悶悶 t adequate t。叫 p. plement propèrly the opaque-2 maize when used in diets w川、 suboptimal levels 01 protein Diets similar 凹的。 se led growing pigs have also been led lactating sows Equa l numbers 01 equal-weight pigs can be produced Irom diets composed solely 01 opaqu• 2 maize plus a proper vi'tamín and míneral supplement. This 。 paque-2 diet replaced the recommended 16 percent crude protein diet based on feeding normal maize and soybean meal A lile .cycle swine product 的n system based on opaque-2 maize was develope::i :m the basis of these resul'ts and additional data from Purdue Uni versity relating to gestating sows. A grε , )hic representation of this system is presented in Figure 1 A substantial saving 01 protein 5upplement can be achieved with this system For examp 悟, per sow per breeding sea-50n , 214.5 kilos 01 protein 5upplem~nt con'taíning 50 percent protein can be replaced by 256.2 kilos 01 opaque-2 maize. Small and family farmers can use such a system for efficient production without bυying protein supplementThe overa ll economics of the system can be determined by consìdering exísting prices within specific areas. If the price of maíze ís high in relatíon to proteín supplements, 'there will be little if any economic advantage in using an opaque-2 maize system; conversely, if The system developed for swine may have greater sîgnîficance for human nutrition . If opaque-2 maize could be successfυ lI y introduced, the added pro le 川 requirements of pregnant and nursing mothers could be totally supplied by opaqυe-2 maize. Infant protein malnutrition could also be substantially lessened by introducîng opaque-2 maize into infant diets Despite the nu'tritional advantages of 。paque-2 maíze, its acceptance has been hampered by ils s。代, floury endosperm (See Maize Production Systems). Samples from a commercial harvest of tw。 Colombian cpaque-2 hybrids, ICA H-208 and ICA H-255 , were observed to be stîll segregatir可 9 with some crystalline kernels appearing among the predom• nantly soft , opaque grains. These crysta 川 ne kernels and completely opaque kernels were selected and analyzed for prote;n , Iysine, and tryptophane content Results are presented in Table 1 The crystalline samples tended to ha ve higher leve ls of protein than the' opaque kernels. The Iysine and tryptoph<Í ne levels of the opaque and crystalline H-208 samples were similar to those of the opaque H-255 samples, although the crystalline H-255 contained significantly lower levels of both amino acids Rat growth studies to evaluate biologically the various selections of opaque, semi-crystalline and crystalline kernels demonstrated tha't the nutritive val ues of the opaque H-208, crystalline H-208, and opaque H-255 were similar. The cryst.lline H-255 , .1 阱。υgh superior to normal maize, was inferior to the dthers in biological value Semi-crystalline H-255 kernels tested followed the amino acid analysis in supporting growth and proteìn effìcier、 cy ratios approximately m 地-way b令 tween the opaque and crystalline H-255 extremes Floury-2 maize Samples 01 double cross hybrids containing floury-2 genes and produced bγ ICA's maize program have been used in nutritional stud阻 s with swine, rats, and poultry. In all trials to date, floury-2 maize has not equalled locally available opaqυe-2 varieties ín growth support Or efficiency of protein utilization The amino acids Iysine, tryptophane, isoleucine, and threonine have been limiting in these floury samples 心 There are indications that some of the floury-2 genes associated with the modifica' Üon in pro tein quality may have been lost in the development 01 the dd uble cross material C\"\".V'I It has been demo nstrated that cassa-Vð can be used as the major SQurCe of energy during t he entire swine life-cycl e Production systems based on either fresh , chopped CaSSaVil ar dried cassavn mea l have been developedWhen dried cassava meal , prepare:l from \"the variety LJanera , was used as the major SOurce of energγlor growing pigs, a small but signi/icant growt h . rate depression cccurred . Thi s growth depression might be related to various lactors such as digestibili ty and energy utilization , hydrocyanic acid toxicity, poorυtiliza'tion of the protein fraction , amir、。 ðcid deficiencies, and possibly to del 時 ient o r marginal leve ls of fatty acids present in diets containing high levels of ca ssava meal Research with growing pigs and chicks has been directed to the identifi cation of the factor c r fac to rs respo n sible for \"t his depression. Swine meta bolism studies demon strated that the dry matter and energy Iraction 01 the cassava meal was di gested at a level 到 mi lar to that 01 commonly used leed ingredients such as maize and soybean meal . Despite this , however, growth depression occur red For swine grow th stud 阻 s , all diets were calcuJated to contair、 16 percent crude protein , of which 8.8 percent cam':! from 'cassava meal , and the balance of protein and energy from .a combination of soybean meal and maize These d 間 ts were 5υpp l e men'ted w川、 methionine, molasses, and beel lallow Data in this experi men t indicate ~hat , in the absence 01 beel tallow (Iat ), meth 悶、 ine supplemen 削。n In crea 叫 Qain s, as did a 10 percent increase ot fat alone to 'the d叫 H oweve r , when both fat and methionine were present , a depression in gains was recorded 26 Consequent旬, w hen soybean meal is us' ed to supplement diets containing cassava meal , methicnine appears to bε a limiting amino ac 肘 thus , correction 。1 th is de/iciency overc omes 'the growth depressioh, . However, i t i s not yet known whether the same response when 10 percent lat is added to the diet is related to a change in the amin 。 acid balance 01 the diet (as the amount 。f soybean meal is increased to main-taÎn a 16 percent protein dÎet) o r t。 an effect 01 lat por so (as lat supplies both concent rated energγand latty acids)Supporting studies with chicks pro. vided additional evidence that w hen cassava meal replaces m 刮目 in a diet supplemented with soybean meal , sesa me meal and fish meal , methionine be comes a limiting ami no acid . As with the above studies, the add ition 01 lat alon9 with methionine into L the diet re su lts in a growth depre ss 咱們。In studi es with pigs and chicks, ad dition 01 molasses sim ilarly lai led 怕 improve gains as when fa't was added, t hυs suppor ting the theory that lat per .0 supplies some fact o r (energy or latty acids) deficier、 in cassava diets Chemical an alysis 01 the nitr。同en fraction o f caSSðva pulp indicates that approximately 50 percent 0/ the nitrogen is not t r.ue protein but 問 present as non-prdtein nitrogen . Although the tot al nit rogen level 01 the peeling is higher than that 01 the pu1p, approximately 70 percent 01 the ni'trogen is non.proteín ni't rogen and therefcre of Ii t tle if any va lue to monogastric animals Preliminary data Irom chick growth studies in progress support the indications that the biological value 01 the nitrogen fraction ava ilable in cassava is poorIn many area s 01 Central and South Ame ri ca, Jarge quantities of bananas and plantains un fi't lor local or export market consυmption are available for Lactating sows nυrsing litters are, however, unabJe to consume ade呵 uate quantities of ripe bananas to me.:~t their daily energy requirements. In addition , consumption of more than 13-15 kilograms per day brinqs about di 月 estfve problems in the sow leading to diarrhea which , in turn , crea 、 es sanitation problems În the farrowing crates T 0 overcome I咐 i s problem and to extend the area of usefulness of reject bananas, s tudies ha ve been carried out to evaluate green banana meal as a possible replacement for ripe bananas. This qreen banana meal , in contrast to fresh bananas, can be stored for lonq periods of time after drying, and can be transported economically to areas of demand far removed from the banana zone Research results obtained in collaboration with INIAP demonstrated that dry green banana meal can replace maize as an adequate energy source for lactating sows , supplying up to 53 percent cf the lactating ration wi'thout reducinq the number of pigs weaned or weaning weights This dry 日 reen banana meal can als。 be used in the diets of growing-finishing pigs. Levels of up to 75 percent 01 the diet have been tes'ted with only small losses in daily gains. Each 25 percent increase in the level of green banana Efficient economical use of fresh or dried bananas instead of maize as the principal energy source for swine production depends upon Iccal prices of prctein supplements and of swine, as well as price per kilo of banana meal , w hich should be approximately 50 percent of that of maize, according to the studies in Ecuador R-ice Increasing rice production in Latin America points t。廿1e possibilitγof its future use as a replacement for maize and other grains in swine diets. In view of this , the swi ne program at CIAT ha s studied the biological value of rice and its by-products in relation to that of 。Þ!ther feeds Results of biological evaluations of w hite rice, brown rice, and rice polish ings, as compared with normal and opaque-2 maize, are shown in Table 3 Both white and brown rice supported gains and demonstrated a feed conver sion efficiency 2.5 times that of normal maize in rat qrowth studies at CIAT. However, nei'ther of these tw。 kinds 01 ri ce equalled the perfomance levels of opaqυ0-2 m 刮目, whic h supported Qains 47 percent higher than those obtained with either white or brown rice Efficiency 01 leed υtilization was similar lor aíl tre.tmen'ts except ground paddy rice, which required larger quantities 01 leed per kilo 01 g. 川, as the indigestible rice hull s were no t removed. from the ration Rice and rice by-products can , there fo re, be used efficiently to replace normal maize in swine diets during the period from weaning 怕 market , il the price of r;ce 悶 below that 01 maize Sugar Refined sug ar has been shown to be an excellent source of carbohyd rates fo r swine. Research has demonstrated that increasing the amolmt of refined sugar in the diet, up to a level 01 45 percent , resul'ts in a cor respondinq improvement in pig perfo rmance and leed efficiency However, as suga r is an export crop needed to balance the dollar drain in most of the producing countries in Latin America , there 時 little possibility th.t it will become available for national swi ne production Reports Ircm Enq land and 'the United States have ind;cated th at sucrose 28 may play an important r。他們 prod u cing high blood cholesterol leve ls and arteriosclerosis in swine, In feeding tri.ls carried Out at CIAT, where high sucrcse (refined sugar) levels were υ揖d , blood samples were taken Irom the pigs and cholesterol levels determined. At sl.ughter, the heart and aort. we re collected from each píg and examined lor lesions 01 latty dεgenerat10n Results 01 these analγses indicate that there were no íncreases in aor'ta lesions .ssociated with this tre.tment , although blood cholesterol levels were somewhat higher than those of control animal s fed diets containing no sucrose A second study comp.red a control die't of normal maize and s。γbean meal with diets containing 10 percent ani ~ mal lat, 60 percent suc 的時. or a combin.tion 01 these two ingredients. The animals remained on these diets for 182 days to allow adequate time lor lesion development. Blood samples taken near the end 01 the study demonstrated that diets containing animal lat signilicantly increased the cholesterol level , whereas the sucrose diet did no t prcduce an increase. No aorta lesions were 刮目ciated with the diets employed in this study .The difference between these results and those repor ted 川 t he previous study may be related to differences in diet . composition, as the first s tudy used purilied diets, while the second used natural ingredients Cowpeas Such protein supplements as soybean meal , fish meal , meat meal , cottonseed meaJ , and sesame meal often prove eCQnomically inefficient in swine diets be cause of limited supply, scarcity, or high cos't CIAT is evaluating other protein SQurces, particularly those that Cðn be grown and used in thc farm with mini-maJ processing. Initial work has been wj'th cowpeas (Vigna sinensis) because of their wide range of adaptability and ease of production. Initital tests with P句 s demonstrated that cowpeas , as 。 ther grain legumes, contained grow 怖, inhibiting factors which limited their usefulness. Results of rat studies indícated that germination , as well as heat treatment , destroys or reduces these in. hibitory factors. When supplemented with low levels of methionine, germinated cowpeas supported results equal to those obtained with a control di e!t of soybean meal Animal Health Swine research in animal heaJth has focused on two principal infectious dis. eases, mycoplasmosis and hoof-and. mouth dis~ase, as well as protein defi.Sarcoptìc mange. a com mon skin problem in protein-deficient swine cíencíes. Although these studies are con'tinuing, some preliminary conclu. sions may be drawn MycopJasmosis results in arthr i't 時 , pericarditis , and pleuritis in young swine. Initial research in this area has been started in collaboration with th~ ICA Graduate Shool at Tibaitata. A segment of the swine population is being sampled for prímary ísolation , and a serologic survey is underway. T。 date, M. hyorhinis has been isolated from swinù having excessive pericardial fluid Hoof-and-mouth disease of swine and lack of an effective vaccine continue t。 result in ser;ous economic losses. A pro. ject to stυdy the pa'thogenesis of this disease, fíeld cutbreaks , the role of trauma in transmission , and production 。f the clinical disease, will terminate in 1972A study made to determine organ regeneration in swine following protein deple!tion was completed in 1970. After an initial period of depletion using a diet of common maize alone, th-ree groups of pigs were fed separate diets containing common maize, opaque-2 maize, and casein as the only protein sources. Following the regeneration period, histological evaluatíon of the di-. .Characteristic lesions of foot-and-mouth di sease in swine gestive tract , liver, and bone from lhe animals in each group was made. Results indicated that opaque-2 maize was particularly effective in supporting regeneration. However, the diet based on protein from casein proved to be Înadequate for liver regeneration , which may possibly be associa1 ted with a significant deficiency of methionine in the casem It had been observed at the Univer s idad del Valle Hospital that epithelial regeneration was initiated when intestinal flora were controlled in proteindeficient pa'tients. In collaboration with the Uni versidad del Valle, CIAT mea sured the effect of antibiotics in t he regeneration of the intestinal epithelium 。f swine dυring protein depletion and repletion. Three basic diets with and wi'thout antibiotics were used. Preliminary results indicate that antibiot 自 cs have a detrimental effect at a low protein level , whereas a beneficial effect is 。bserved with nutritionally adequate CIAT' s rice program 咕。 riented t。 一 ward the solυtion 01 problems limiting rlce y 阻 Ids in Latin America. Although many lactors affect yields thrbugh。叫t the hemisphere, the need lor research is particularly critica l in three areas prodυction 01 h 旬 h-yielding dwarf vari• ties having superior cooking and milling quality; incorporation of stable resistance to the rice bJast disease; and develcpment of cultural practices for respcn 剖 ve varieties grown under both irrj. gated and upland condi tions During 1970 the effects 01 the green revolution started to be felt in Latin America through increases in rice p 巾, duction because 01 the use 01 high-yielding varieties, especially IR8. The Uni-'ted States Department 01 Agriculture estimates that 91 percent 01 Cuba's 叩門 ng c rop (105,000 hectares) was pJanted with this variety. The same Vðriety was planted on about 20,000 hectares ' 2ach in Colombia and Venezuela and on appr。削 mately 5,000 hectares in Ecuado r IR8 and IR5 occupied 12 .5 percent 。 f Peru's rice area and, for 'the first tìme in years, Peru is nearing self-sufficie r: cy 附 rice produc tion . It is likcly that Co:onlbia would have had to Import rice if It were not for the excep . tional yields 01 IR8. It is questionable, however, whethe r IR8 wi ll continue t。 íncrease markedly. As c。υntries achieve sel! -sullicier、 cy Or have small 的。 cks lor exp。門, the price lor IR8 will decline rapidly because of its inferior grain quality RICE BREEDINGFollowing the specilic breeding objecti ves detailed in the 1969 report , a total 0189 crosses was made in 1970.01 these, 36 were single crosses of dwarfs using various selections o1 1R579, IR6óS, IR841 .nd IR930 亡。 lombia 1, a line highl y resis tant to the blast disease in Colombia and .t the Intern.tional Rice Research Institυte (1 RRI) in the Philippines, was used as a parent in 42 single and line X F, crosses. The highly blast. resistant varieties Tetep, Marr、。 riaka , 。旭日 H.til, and C46-15 were used as parents in 11 crosses wi 't h selections of 1 R665 and 1 R841 Nurseries Six plantings 01 segregating material were m.de during 1970 a't the ICA experiment station at Palmira (January, March , May, July, October, .nd Novemb.r). These included 13,462 pedigree rows that were evaluated for vigor, earlinesses , plant type, cooking quality, grain appearance, and resistance to 50gatodes, hoja blanca, and blast. AII material in the program is now dwarf, and the majority has acceptable grain char acteristics . Fixed lines (F 7 ) from the lirst crosses made in 1967 will be advanced to preliminary yield trials in 1971 Additionally, 80 F, bulks 01 4,000 t。 6,000 plants each were grown and Solected. Those bulks having a blast-re-剖 stant , tall.statured parent were thor-oughly rogued 01 all tall plan'ts after flo we ring to allow survival and sel 配 tion of tne d warf segregates. Failure t。 cut out the 'tall segregates in directly sown bυIks would result in the loss 01 the desirable dwarf plants through competition for light F。υr international blast nurseries with 356 varieties each , and four 01 blast modera'tely resistant varieties with 212 each , from IRRI , were planted for evaluation at ICA's La libertad 5tation Some of these varieties we re already present in the CIAT nursery and, thus, ù ccmbined nursery was formed which will be distributed to Latin Arnerica in 1971 Yield trials A 'total of 289 va rieties and lines was grown in unreplicated observa tional yíeJd trial s. The majority were introduced from Brazil , the Philippines (IRRI) , Mexico, and the United 5tates Department of Agriculture. The USDA mate rial , compri 引 ng ncw promising U5A lines , was received specifically to test its resistance to blast , hoja blanca , and Sogat 吋剖. AII of the Brazil and the U5DA material was discarded because of low tillering and tallness. The Mexico and IRRI lines yielded well , nearly all being dwarf, but most were eliminated because of lateness and p∞ r grain tra i'ts One hundred forty replicated y 陪 Id trial entries were evaluated . Outstanding material included s, electìons from the following crosses: I R8x (Petaj5 x Belle Patna ), (Petaj3 x TNI) x Kha。 Dawk Mali , ond IR8 x IR12.17日 -2-3Fifteen se!ections of the most promisi ng long-groin dwarfs selected during the oast three years were grown 2 and 3, respectivelyThe upland results in Table 3 are part 肥ulariy noteworthy. The test 川 Paη-ama included 84 lines, 01 which IR930-31-1-1B gave the third highest yield Five 01 the 10 most productive lines were selections 01 IR930 . The 10 lowest yielders were all selections 01 IR665 Additional evidence that IR930 is adapt. ed to upland culture is the high yield 。 1 IR930-31-1-1B in Costa Rica. In a preliminary test at Tumaco, CoJombia, the same line yielded 4.9 ton j ha. This was 2.2 tonj ha greater than that 01 any 。 ther entryRepeated inspection of the trials reported in Table 3 and elsewhere led CIAT and ICA to jointly name and re. lea 阻 1 R930-31-1-1 B as the variety CICA 4 to be released next year . This variety was obtained by three cycles 01 selection of segregating ma'terial received from IRRI in 1968.ICA has about 45 tons 01 seed 01 CICA 4 01 which the major portion will be multiplied by registered seed producers in Colombia. The remainder will be distributed by ICA to small , marginal upland rice farmers About hall 01 the 10 tons 01 breeders' seed 01 CICA 4 produced by CIAT will be distributed outside 01 Colombia. The remainder is being planted in one hectare regional trials in Colombia In nearly 011 te5t5 01 irrigoted rice in Colombio and Ecuodor, the line IR665-23-1-1 B ha5 yielded more 'Ihan other se-lection5 (Toble5 1 ond 3). 1I will not be released , however, becau s-e its production of unbroken grains during mil. ling i5 rel 的 ively low (Toble 2) A5 individuol plant5 appeared to dilfer in milling quality, several hundred plants were selected and evaluated individually. Ab。 υt one hundred 01 these will be multiplied ond reevaluoted for milling quolity in 1971The tWQ lines in multiplication , 1 R665-33-5-8-1 B ond 1 R665-33-1-3-1 B CIC~ 4 rice grown under upland cullure in Ecuador (Photo court臼y of INIAPl.(Tables 1, 2, 3) were discarded as being inferior to CICA 4 and IR22A number of crosses involving CICA 4 and related IR930 select 的ns are beíng screened to identify lines that have earlier maturity, leaves that remain green until harvest , tighter threshing , anâ slightly less amylose in the endo sperm have kept their resistance 'through 14 plantings from April, 1969, through De cember, 1970, in the Ll anos and the northern coast of Colombía. 'However, a test carried out in the jungle of Peru showed that aboul half of these varieties were susceptible. This points to the need for further testing of these varieUes un der conditions favorable to the develop. ment of the disease Preliminary results of a .tudy of the nature of a horizontal resistance of rice to p ,。吋Z鷗 indi cate that susceptible varieties have more type 4 lesions, and in general more lesions than moderately resÎstant varÎeties. The averag~ size of the lesion in millimeters for Fanny (susceptible) was 29.1; Bluebonnet 50 (susceptible) , 11.0; Perola (moder-.telγ 闊別 stant) , 4.2; and Colombia 1 ( res 時 tant) , 1.9. Sporulation started ear lier and lasted longer in the susceptible va rieties ðS compared to 'the , res.is tan. t ones, and the number of spores produced wa5 significantly lower in those moderately resistan t. Fewer les;ons per leaf area, longer time to start sporulating and fewer spores produced ' by a lesion dυring its lifetime are three common features of va rieties having hor卜 z。nta|reslstance \"New fυngicides a~d methods of application for controlling rice blast were 36 U also studied. Direct applications to the s。刊。 r to the ìrrigation water, as well as combinations of s時d , 50il , and foliage ap闕 ications, were 峙的 ed , Five fungicides widely used in temperate zones to control this disease are being tested in the field in plots of 5 X 3 m到ers An experiment is also underway to test 40 fυngicides in screening plots of 1 m'. Re5ults will be reported in 1971In a preliminary experime巾, the 5yStemic fungicide Benomyl applied at 40 。 r more kg/ ha pr叫配 ted the rice plant from infectionυP to 300 days under continuous cropping , Thi5 wettable powder, or other systemic fungicides , applied in a granular form to the soil or to the irriga'tion water appears to offer an efficient and relatively low C05t control when combined with • parti.lly resistant varietyA previously developed method of isoJation and purification of this virus was improved. An antiserum was obtained which will ba used internation-.lIy to search for possible strains . 01 the virus. Electron microscope studi肘 。f the nature of the transov.ri.1 passage of ' the virus through several gen. erations of the vector Soqalodes ory. .;col. are being carried out in collabo. r.'tion with the University of Hokkaido, J.pan Information exchange on resistance to rice blast has been started with IRRIRice agronomic research at CIAT was expanded in mid:1970 with the addition of a rice agronomist to the staff. Research ;n rice agronomy has . been con. centrated on ob'taining information about cultur.1 practices appropriate t。 the new varieties produced, before releasing them to farm~s under Breeding ,的 e five most promi 別 ng I i nes were compared to tWQ widely grown varieties, IR8 and ICA 10, under uniform cultural practicesResυIts show that two of these new lines, CICA 4 and IR665.23.3 斗 pro.duced higher yields than IR8, while the other three produced Irom 80 to 86 percent 01 the IR8 yield. The yield 01 ICA 10 was also higher 'than IR8. 0η commercial farms , however, yields of this variety are generalJy much lower because of its sυsceptibility 10 rice blas t.Five different levels 01 nitrogen (0 , 25, 50, 75 and 100 kg j ha) and tw。 levels 01 P,O. (0 and 50 kg j ha) were applied to CICA 4. One-half 01 Ihe nitrogen and all 01 the P,O, were applied 33 days after seeding, and 'the remainder 01 the N was applied 52 days after seeding. Two additíonal treatments were included in which all 01 Ihe ferlilizer was applied 33 days after planling.Allhough there were some differences in height and maturlty because 'of the hlgher ra'tes of nitrogen , there was 。 nly a slight response to nitrogen at the 50 kgj ha rale and no response 1。 phosphorus. Yields were depres叫 W the higher rales 01 nilrogen. There did ndt appear to be any advantage 10 mak. ing more than one fertillzer applicalion 10 CICA 4 on Ihis lertile soil Rales 01 seeding Opinions differ greatly as to the correct amount 01 seed to use lor direct seeding 01 rice. It is generally believed that värieties which-do not produce a large number 01 tillers should be plant ed at a higher rate. To gain informa-'tion on this matter, two different types 01 rice (IR665-23-3-1-1B and ICA 10) were grown at widely differing rates 01 seeding Yields of both varieties were slightly lower at the 25 and 50 kgj ha s肥d ing rates. There was practically no difference in yields between the two types 01 rice at the higher seeding ratesAlth。υgh the resul'ts Iror甘 this ex periment show that it is possible to obtain high yields w 內 h much lower seeding rates than are commonly used, it appears desirable to use from 100 t。 Under the Crop Production training pro~ gram, students obtain practical experience and knowledge from CIAT's technical staff 38 125 kgjha on commercial plantings because 01 increased weed competition at lower seeding ra'tes. Rates higher than 150 kgjha seem to be unn缸essary and increase production costsIt is a general practice in Colombia to make three to lour applications of nitrogen to direct-seeded rice crops. An experirl!ent was designed to de'termine the most effective times lor applying nitrogen 10 one 01 the new lines, IR665-33-5-8-1 B. The applications were timed to coi ncide appr。別 mately with the 101lowing growth stages 01 this variety: Tillering Panicle initiation Booting Heading 25 days after seed 川9 60 days after seeding 67 days after seeding 77 days after seeding Results indicate Ihat no benelit was derived from incorporating the nitrogen in 'the soil prior to planting. As there were no significant differences between this tre的 ment and the unfertilized plot, it appears that the nitrogen was lost before the rice plants were sufficien'tly develop叫怕 use rtThe most effective treatments were th。但 in which 50 kg j ha of nitrogen were applied 25 days aft.r seeding , during the tillering stage. There was no benelit Irom the application of additional nitrogen during the panicle initiation , b∞ ting or heading stages Effecl \"l oil molsture 0\" nitrogen response Low availability of nitrogen is one of the principal lactors limiting rice yields in many parts of the world. _ With transplanted rice it has been lound most effective 10 incorporate the nitrogen in the soil just prior to transplan'ting. However, such applicati<:>~s with direct-seeded rice are not possible without damaging the rice planls. The two common methods of application are to incorporate it in the soil prior to planting or to broadcas't it in the growing rice as a top-dressing In an experimen't designed to com pare the effectiveness of these tw。 methods on the soil of the CIAT farm using 1 R665-23-1 -1 日 and a nitrOQen rate of 100 kg/ha , it was f。 υnd tha't the application on dry soil produced the highest yield. The lowest yield oc curred on the plots in which nitrogen was incorporated before planting. Yields 。btained from the applications on wet soil and in 'the water were 87 and 70 percent of that obtained from the application on dry soil. To equal the yield produced by the dry soil application , 140 kg/ha would have to be applied in the irrigation waterExperiments are continuing at the Carimagua station , where flooded rice has been successfully grown for two semesters , with yields of more than six ton/ha in the second semester. A num ber of managemen't problems have been solved, including soil puddling, pr• plant flooding , and water seeding. Considerable progress has been made in describing the chemistry of flooded oxisols. Iron toxicitγ , which appears t。 be a limiting factor for flooded rice, may require pre-plant flooding for satisfactory results The primary objectives of the maize program at CIAT are to increase production and to improve the nutritional quality of maize throughout the lowland tropics , with emphasis in Latin America Maize is an important ingredient in the diet of Latin Americð. For exam. p 峙. estimates of maize consumpt 叩n from Central America indicate that 60 percent of 'the calories and 50 percent 01 the prote川 come from maize In Colombia , maize supplies approximately 30 percent 01 the to'[al caloric intake. Maize production is increasing, partly because of an increase in land area dedicated to the crop, and partly because 01 higher yields per hectare Average produc'ti。代 however , remains between one and two ton5 per hectare Mor閻明 r I many farmers operate at the subsistence level. More than half of the farms engaged in maize prodυction have less than five hectares, and more than 80 percent cultivate less than 20 hectares . In most cases the crop is Çj rown and consumed on tho farm , and only about 20 percent of the crop enters commercial char、 nels Migration to urban centers accentuates problems of lood supply and nutrit 的n . Preferences for maize and maize products, as well as particular tastes for certain types of m訓 ze, are 臼 rried from the rural areas into the city. New maize varieties or hybrids with greater productivity and improved quality must incorporate grain characteristics which 40 are known and accepted by 'the intend ed customerDuring 1970, a concerted effort was made to bring in commercial hybrids, open-pollinated varieties, and variable popul 剖 ions from maize programs in Asia and the Americas in order to test these materials in the Andean zone and make them available to nalional p 巾, grams. Composite populations among these introductions include material from 叫 idely diverse commercial s。 υrc肘, as well as maize from the germ plasm banks. These mÎxtures and new 。 nes will hopefully contain a potential for wide adaptation and a relatively wide resistance 'to disease and insects AII these maize introductions will be grown in observation plots dυring the next year, and promising indivíduals and selections will be combined lor further observation and testing Early progeny testing and recombination of lines in improved maize popula'tions led to success in Kenya and appear to promise substantial improvement in CIMMYT's international program . CIAT wilJ begin with several basic populations (including opaque, brachytic, Andean-zone low altitude, Andean-zone high altitude, and the world composite ), and test early selec'tions Irom the目 populations in a number 01 locations in collaboration with ICA, INIAP, and other n.tion.1 progr.ms. These locations will include ð minimum 。f three low-elevation and one intermediate e'levation sta't ions. Evaluation at high elevation stations will be accomplished by national programs in the zone 州的 t recombinatÎ on wi ll be done in the intermeciate altitude of CIAT headquarters in Palmira (1 ,000 meters) , and further selections tested in the several regional stat 巾的In addition to the concentra ted effort in maize, a small nursery for sorghum introductìons was planted in 1970. Germ plasm collections, promisîng vari肘 îes , and commercial hybrids have been requested from programs ín several continents. These geno'types wi lJ be planted in 1971 , observed in the nursery, and selected lor desirable plant type and early maturity. Prom 山 ng selections wi ll be available at any stage 。 1 development to national 阱。gram s and commercìal companies in the trop-Wider adaptation of varieties or hy_ brids can lead to greater use of improved maize types by bringing these t。 a wider / range of micro-climates. T 卡 IS will also redυce 'the number of commercial materials and minimize complicated and expensìve seed production procedures , Factors which inlluence adapta-'t ion include photoperiod and temperature sensitivity, wide-based resistance t 。 insects and pathogens, and a mínimum susceptibility to drouth or high water leve 峙, strong winds, and other adverse climatic conditions Work on pho'toperiod insensitivity contin lJed during 1970, and several relatively insensitive lines were identified in the lield , The photoperiod sensitive reaction in maize appears to have tw。 critical light intensity levels . There is a marked delay in different 叫 ion and flowering when sensi'tive genotypes are exposed to intensities greater than five to seven 1。咽 tcandles , and the same materials are delayed s lightly at intensities between one to live loo'tcandles. In these stuclies , lower ìntensities had no ap pa ren t effect Preliminary results indkate a critical day length 01 14 Y, to 15 hours lor maize. Quantitative interactions between day length and light intensity have not been s'tudied , There is no apparent elfect 01 day length on gro\"';th rate as long as the temperature~is constanl for contrasting day length conditionl_ A c~~pe ~~~i.~~. .pro ject is being planned with CIMMYT to study a uñiform se't 。t materials at three altltudes in C。 lombia and three altitudes in Mexic。 怕 explo:e .the interactions of temperature and photoperiod sensiJtivity Excessive plant height and resultant lOdg~n~ sever~.?' limfts yield in many coastal areas (Photo taken. at Pichi1íngue, Ecuador)Efficient energy conversion into plant dry matter and grain are crucial to in~ creased productivity per hectare. 1 n the tropics, where a year~round growing potential must be utilized, yield evalua tion should include production per hectare per day, per unit leaf area , and per unit 01 Iigh't intercepted. Recent advances in the measurement of light , leal area, and physiological maturity have made it possible to measure more of these efficiency parameters at a low er cos t. A trial to evaluate the effect 01 luradan , a systemic insecticide effective in roo'tworm control (Oiabrotica sp.) , was made to determine whether this chemical would also offer residual in secticidal properties against the lall army worm . (Laphygma sp. ) attacking the whorl 01 the plan t. Evaluation 01 army worm damage in the early growth stages shows that luradan, applied in granular lorm to 'the whorl 01 the plant or as a foliar spray, was more effective than the conventional granular chemicals applied \\0 the plant whorl. Furadan applied to the soil was not effective. Às the plant developed, the residual effect 01 luradan did not prevent army worm damage to the new leaves Insee:t damage at this stage 01 growth may not appreciably affect yields Typical maize culture in the small farms of -the Andean zone. Maize is often combined with other crops (in this case with beans, in the highIands of Ecuador)CIAT must work with national agencies m order to reach the marginal farmers with improved varieties and practical production systems adapted to their conditions Furadan applied to the loliage was 刮目 effectiye in preventing leaf damage to young plants by the adult rootworm beetle. Ordinarily,的 is damage does not merit control measures In this test , severe lodging occυrred in the lield after tasseling due to heavy winds and rains. There was sìgnifican't-Iy less IOOging in the luradan-treated plots 'than in the non-treated ones Yields were higher in plots treated with higher rates 01 luradar、 bυt the overa 11 yield level was so low that the prolit ability of such a treatment is quest 的 n ableA multidisciplinary analysis of the factors associated with low maize yields in Colombia i5 in progress. The objec. tives 01 the study are 1 ) to identily important factors associated with low maize yields among small farmers in certain selected reg昀肘。1 Colombia, 2) 'to determine the interrelationships among these lactors, 3) to suggest avenues 01 approach to the problem 01 increasing yields, and 4) to develop a methodological Iramework which may be applied in similar studies in other reglons Information has been obtained from t h ree sou rces ﹒ 的 small larmers, b) input suppliers, and c) local agricultural extension and lending agencies. Personal ínterviews, field observa1 tions, and soil tests were used as means for obtaining the inlormation. Analyses 01 these re> sults will be completed in 1971 and the study will be extended to other c。υn tries in the Andean zone. The objectives of the study were t。 identify the main obstacles 'co a $uccessful expansion of the production. 'marketing. and human consumption of 。paque-2 maize. and to sυggest ways by which these obstacles may be overcome Basic datð were obtained from interviews with produce 悶 marketing a.gencies, apä ' consumers It was found thàt a rapid expansion of the product 叩n . ànd 祖SOJJS.u. 111ption of 。 paque-2 T maize was limíted by: 1) low consumer acceptance, 2) wholesale 悶, lack of interest in handling the product.3) low relative farm yields in comparison with high-yielding hybrids. 4) low producer acceptance among small farmers. and 5) storage prob lems . These items directly relate to the flou 吋 a-nd soft endosperm of opaqυe-2 ma ize, as compared to the commonly grown .flint-. type maize. The softness of opaque-2 results in low resístance to insect atlacks, low kernel densi'ty, and undesirable appearance and cooking characterislics Results suggest that sυccess in In tro . ducing and expanding commercial production for human consumption of the present vari.Hies of opaque.2 would ck.pend on a considerable governmer甘 sub. sidy to farmers in conjυoct10n with prom 叫的nal campaigns among producers. marke't ing agencies, and consυmers However. if a flint type high.lysine maize with a high.yielding capacity could be developed. government support cou ld be reduced or even eliminated Although home consυmption of the present fl。υry opaque-2 maize among low income farmers could be prom叫 ed al a somewhat lower government c。肘. such a program would demand a concentrated extension effort, and provision should be made to help the farm. er expand his production of ordinary maize or some other casn crop to cover the cost of opaque-2 seed and other additional inputs.• .,.Improved protein quality in opaque-2 maize will be of little advantage if peop~e do not accept the floury-type endosperm (below): the kernel on top has been selected to combine a flint endosperm with high quality in the grain.MAIZE PROTEIN QUALl TY 1 . Procluctlon of f1i nt.type opaqu...2 maize.The nutritional advantage of opaque-2 maize gives this product high priority in CIA T's genetic improvement pro.gram. 1I the genes which modily endosperm type can be identilied and concentrated in maize without losing the protein qυality advantage 01 the opaqu,• 2 major gene, many 01 the prodυction , storage, and acceptability prob lems associate d with present varieties can be elimina'ted. As a first step toward 。btaining a more desirable type 01 opaque-2 maize, Ilint and semi-Ilint grains from commercial harvest of the 。paqu令2 have been selected and also from lines , single crosses, and other backgrounds wi'th the opaque-2 gene Preliminary results from laboratory and biological stυdies are presented under Swine Prodυction Systems 2. Yields 叫。 poq ue-2 maize A lield program was launche d in 1969-1 中 70 to eva1uate the production 。f opaqυe-2 commercial hybrids on the farm. Traditional cultural methods were compared with improved technology, ïncJuding use of fertilizèrs , weed control , In sect contr訓, and irrigatiòn where possible. Thi s study compared the new 。paque-2 hybrids with regional varieties, and with the recommended hybrid for each zone. Six Colombian aoron。 mists assisted farmers in planting 110 trials in two seasons in 14 different departmen'ts of the country In the trials harvested, opaque hybrids were the highest yielding in 15 locations. Over all locations, the recommended normal hybrids yielded 14 percent more than the opaques. More significantly, the opaque-2 hybrids yielded more than 40 percent over the re-9ional varieties, e 川 her under . improved or tradìtional cul'tural methods . From these results, it was apparen~ that replacement of traditional variefies by an 。 paque.2 hybrid would siqnilicantly increase both the yield and the quality 。 f a small farmer's maize crop .3. Effects of altitude, temperalure, and 個 1.. 側ergy On qual1lyA cooperative study has been initia't-ed with Purdue University and CIMMYT to evaluate the effects 01 altitude, temperaturej and solar energy on p巾, tein content and qual i'ty. This is a liveyear study 01 adaptation which als。 can lead to a better understanding 01 plant growth and efficiency 4. N叫 rilion work wilh opaque-2 malz.Sludies con'tinued duri ng 1970 on the r目 uperation of undernourished chil _ dren. This work is carried 0'ut by the Department 01 Pediatrics 01 the University 01 Valle and the Metabolic Unit 01 the Departmental Hospital 01 Va l]e. This labora'tory has continued to co1laborate with b0'th ICA and CIAT in the analysis of protein and Iysine in maize samples. Research on several manufactured loods has continued with the co-。 perati 0' n 01 private industry. Duryea, a baby lood made by Fruco-Maize悶, S. A., uses opaque-2 n、 aize as a source 。f protein . Further results in the use of 。paque-2 maize in swine nu'trition are presentedυnder Swine Production Systems PROMOTION OF OPAQUE-2 MAIZE Although there are field production and marketing problems with the present II。υry-e ndosperm opaque-2 hybrids ,的 eir tremendous biological value suggest s an immediate rural extension effort to begin to alleviate rural nutrition deficiencies A cooperat 叫 e ICA-CIAT project was planned t0' promote the adoption 01 the present commercial . opaque-2 ' hybrids among small farme[主 i.n Golombia , and to stimulate its indÙstrial ' utilization in an effort 'to improve nutritional levels among urban populations . To attain these objeçtives, the project w刊 l 剖e tempt:1 ) To achieve in s'titutíonal coordination and integration of 'the various agencies working in the agricultural sector 01 Colombia; To examine strategies for the promotion of opaque-2 maize, a meeting was held with CIMMYT specialists in Mexi 司 co. CIAT staff members also met with 。 fficials 01 'the Ministry 01 Agriculture 。1 Panama, along with representatives Irom o~her agricultural organizations, 怕 discuss seed production and emph.size the nutrition.1 advantages 01 。 paque-2 maize. During the past year, samples 01 the two opaque hybrids have been sent t。鉤。 ut 35 ∞ untries in Latin America and thr。υgh。υt the world.Training in maize production and im. provement continues to carry a high priority. The six Colombian agronomists who conducted regional testing 01 。paque-2 hybrids completed their training in 1970. One agronomist worked as a trainee in the maizE卜breeding program lor lour months belore joining the CIAT st.ff as a rese.rch assistant. A scientist from Haiti will soon finish his master's degree in Mexico and join CIAT's m.ize program as • rese.rch lellow. An Ecuadorian agronomist will join the group 01 research lellows in 1971. In .ddition, training in the .gronomic aspects of maize produc'tion is an integral part 01 the crop production specialist training project reported un der the section of Trainìng and Communicatlon Research results in maize production systems are an in legral part of CIAT's training program.Much 01 the work at CIAT is directcd toward developing, 'testing, and establ ishing viable production systems for specific commoclities -beef, swine, rice, m 刮目, food legumes, and CðSSaVa Practical considerations dictate specific attention , as well , to the diverse multi-commodity production opera'tions already to be lound in the lowland trop ics or which are likely to be acceptable, economically sound , and viable in certain tropical environments Consequently, the CIAT s'taff considers such significant issues as these: a) rota'tions between and among crops; b) ∞ mplementary animal-crop system s; c) mυIti-c ropping and inter-tillin 日 and d) the complementary and competitive aspects of various commodities with respect to allocati。的。1 land, lab。人 credit , machin ery, power , wa'ter, and 。 th er resou rçes. Other con 剖 derations must include domestic and export rnarkets, the local marketing and processing potentials , and overall , the relative benefjts of various systems to individuals and society. As a consequence, the ra :1 ge 。 issu. es wi th which CIAT must contend, ei'ther directly or thr。υgh national , regi ona l, and other ín'cernational agencl 間 is broad and complexVarious maizc and soybean intercropping schemes w ith Ll anera cassava were planted to test 'their possible advantages under tropical conditions S叫 t reatment s were set up, three using the cassava planted into already Production Systems cxisting stands 01 ICA Pelican soybeans, 51 days after the latter was planted; two involving simultaneous plantings of cassava with either soybeans or H-253 maize; and a control plot of cassðva planted alone Cassava cuttings, 20 cm in length , were planted at a 45 degree angle, 180In the tropical lowlands the multiple cropring system offers many advantages to the small (armer (left). A ñame plant (cenler ) grows in a field cultivated with maiz.e and cassava cm between rows , and 110 cm between plan 峙, for all trea't mentsResults are presented in Table 1, Cas sava γields were lowered by the competition for light , water and nutrients from the companion crop; however , t。而 I gross return was quite similar for all cropping systems. If cos'ts of pro duction are considered for the double cropping systems , the dìfference in net return between the various systems would be very slight Production of cassava planted in exis-'t ing stands of s 。γbeans was apparently related to the amount of space available for plant growlh. Yields were highest when cassava replaced soybeans in the row (Treatment 3 ), and lowest when cassava was plan'ted 叭怕 the soybean rQw (Treatment 5). The cassava was more competítive than the soybeans when both were planted at the same time, and tended to crowd the soybeansThe maize companion crop grew con siderably taller and faster than the cas sava. Maize competed for a longer time than the soybeans planted carlier than the cassava Sorghum manag . ., ment 叮 stemsTwo varietíes of sorghum , P-25 and ICA-Pal-l , were planted in a lield tr 崎 l to evaluate length of sorghum survival through regrowth under different levels of managemen t. Plo'ts wíth and without irrigation were planted for both varie ties. ICA-Pal-l lailed to give sufficient regrowth after the first harvest for further harvests. Various treatments w. ~re applied to the remaining írrígated and non-irrigated plots 01 P-25, as lollows 、 1) fertilization and fìeld cu,ltivation; 2) fertilization alone; and 3) neither fertilization nor field cultivation. Yields in 'this experiment are presented in Table 2.Higher yields 01 'the non-irrigated treatments of the regrowth can be ex.plained by the lact that the treatment under-irrigation matured ccnsiderably earlier, resulting in an estimated 20 per cent loss of seed because of bird dam. age. The non-irrigated plots did not sυffer as much bìrd damage because seed was produced at a time when 。 ther fields were maturing, providing larger areas for the feeding birds 80th for the irrigated and non.irriga ted regrowth pl 。峙 I field cultivÐtion ap pears to have resulted in lower yields when compared to the plots that were lert 山 zed only. This may be explained in terms 01 root pruning by the lield cultivator. Differences were qui'te small Additional field observations wÎthin 咐 is experiment point to the fact that ncrmal combine harvesting results in a considerable 105s of seed , which then germina'tes and produces harvesting problems 01 the regrowth because 01 a w ide variation in maturity Grain and forage residue production In another experiment designed to evalυ 剖 e sorghum grain and forùge res idue production iJ t djfferent leve!s of matυrity , it was found 'that some varie ties are well-suited for early grain har. vest with a 25-3 日 percent moisture con tent. A system should then be used lor recovering the stalk and grain residues from t he comb川e . In 'th 時 manner , so;-ghum regrowth would permit a 5econd No visible improvement was observed in the borax treatments. However , yields were slightly higher when compared with the urea treatment s. There were no apparent cumulative effects when boron and nitrogen were applied togetherMaize is much more severely affected by boron delicier可 cies than adapted sorghυm varie ties such as P-25. An application of borax was made on part 。1 a production lield with little or n。 apparent effect on the first maize crop S自由 d aher application. Oifferences between no boron treatments were drama'tic in the second crop of maize. The lack 01 response by the lirst crop is ndt enti rely understood but was probably associated with inadequateηcor poration in the soi l. Boron has been known to be limiting lor allalla and citrus on many Cauca Valley soi ls for a number of years but had not been 50 reported as limi'ting for either maize or sorghum Soybean management systems Irrigat-ion and soil variabilíty Two recently-Ieveled lields were planted to ICA.Li li and ICA-Pelican sovbeans to observe soil variability 1011。心 ing leveling and to evaluate the effects of irrigat 叩門。 n soybean produc'tion Soybeans ;n the irriga 臼d treatments were planted on rai sed 50il beds sh aped with a bedder lo llow ing the lo rmation of ridges with a lister-type cultivator. Two rows, 70 cm apart , were plan!ted on each bed . On e furrow irrigation was made at flowering The effects of irrigation were shown in both va rieties by a considerable yield increase. I n one of the two fields occυ pied by the experiment, however, soy-bean5 we re poorly developed and d 悶 played symptom5 01 physiological stress These symptoms were similar 怕的。5e observed in plants inlected with 50ybean m。呵呵。r damaged by 2, 4-0, and 。 ccurred in rather large patches in the lield . Cau5e 01 thi5 phenomenon has not ðS yet b曲 n identilied , although as the same symptoms appeared in the same areas upon replanting, this would seem to rule out the possibiJ ity of an infect 悶 us disease unless it were a soilborne o rgani s 們nThe chemical DRC-736* showed prom. ising results last year for controlling bird damage caused by lhe eared dovo (Zenaida auriculata) to emerging soybean seedlings. Trials this year provided more evidence of its effectiveness In previous trials , 't he chemical was mixed with a s't icker.spreader substance (Roplex AC33) to provide better ad卅日。 n 01 the chemical to the seed The wettìng actlon of this substance caused some damage to the seed coat Consequent 旬, carn oi I was s'tudied as a substitute lor Roplex AC33 . The com. bination corn oil . Phosphorus levels are low 1n the so1ls at Carin、 agua (3 ppm wrth Braγ11 extractant). A few species of forage crops can be establishcd without added phos. phorus from fertilízer; but establishment is usually slow , and excessive seeding rates are required to get good lirst season stands. Applications 01 75 kg/ha 01 P,O, resul'ted in rapid s自dling establishmer、 t and development 01 mo~ lasses grass (Melinis minutiflora) using 12.5 kg 01 s開d/ 怕, about hall the rate locally recommended. Large plots (176 ha to'tal) for a grazing tríal were established with three fertilizer treatments 1) nc lertilizer; 2) phosphorus; and 3) phosphorus plus potassium. Fer. tilized plots were ready for grazing three to 10υr months after seed ing , whereas non .fertiljzed grass normally reqυires a lull year belore grazìng Three experiments were condυcted using herbicides to control native sod , and then introduced species were surface-seeded with no mechanical seed bed preparatìon. De lapon was 'the on ly herbìcide tested whìch ∞r、 sistently gave good control of the native grass composed prìmarìly 01 Tr.chypogon vestitus and Axonopu5 purpurei i. . Where control was adequate, satisfactorv stands of molasses 9日 5S were obtain2d, and, in some cases, seeding of Stylosanthes guyanensis was successfulThe estab l 時 hment of grasses in this manner is of interest from several standpoints: 1 ) erosìon hazard would be much less on rollìng land su rfaces than with mechanìcal seedbed preparalicn; 2) machinery investment could be kept to a rninimum, wi'lh herbicide being custom-app lìed eìther bγδirplane 。 r ground equipment Or with s mδ I! er , non-motorized equipment; 3) rnuch less time wou ld be required than with mechanical secdbed preparation; 4) reduc. tion of the overaH cost of seedling e-s tab lìshment mìght be possìble by sυbtit 叫 in9 chemical for mechanical control Es'tablìshment 01 the lorage crops was slow even on plots where excellent vegetation control was achieved , probùbly because of poor seed-soil contact Fυrther trials are projected in which a rolling rìpple-blade coulter will be used to prepare a narrow strip of soil where seed and lertìlìzer wìll be band 甜In another experiment , a number of legume and grass species were seeded alone and in limited mixtures at three fertility levels , with conventional seedbed preparation. Preliminary observations indicate that several spec 惜 s were easily established, even with no fertilizer or with phosphorus only . The most prom 自 sìng legumes ìnclude Stylosanthe5 guyanensis, Caliilpogonium muconoides, 52 Desmodium in!ortum , and Puer.ria phue叫。ides . The grasses whìch show most promise for this ar目 are Melinis minutifl。間, Hyperrhenìa rufa , and Bra . chiaria decumbens.Two major problems have been encountered in growing rice on ox 悶。 Is The first is one of reducing permea. bìlìty, often as hìgh as 1,000 mm / dav ìn Ireshly plowed L1 anos soìls . Permeabil. ity must be greatly reduced to grow flooded rice s uccess fυIly . The hìghly aggregated soil at Carimagua was puddled with a mounted rotary tìller working in wa'ter, and infiltration was sub. stant 開lI y reduced . The lìrst crop was grown from March to Jυne , and, after the raìn s started, the pump was requ ìred on ly once for 45 minutes to maintain a constan t depth 01 water ìn the lìeld.The dther problem ìs a physìologìcal disease known as \"anaranjam iento\" or \"orange leal\". Gr但 nhouse and labora. tory research result s obtaìned ìn 1970 support the view that iron 。別 city 悶 in part re sponsible for this disease. Extreme phosphorus defìcìency also appears to be involved. Preplant Iloodìng for three weeks allevìated 'thìs problem to some exten t. Floodìng and subsequent reduction normally bring about many changes in the 50ìl , inciudìng a rapid increase in pH , decrease in Eh (redox potential), and an initial increase in iron concen1 tration in the soil sol. ution. After an ini1ti 訓, and sometimes sharp and rapid increase, iron concentration usually decreases to a level intermediate between the initial level and the h 旬 h reached in most inorganic soil5 W 自 thìn tw。怕 three weeks.A series of greenhouse experiments on Eh, pH , electrìcal conductìvìty, and soil solution concentrations of iron , manganese, and phosphorus ì n I1∞ded 。xisols indicate that the soils at Carimagua are quite low in fertility and thatυpon llooding reductìon proc揖ds very slowly untìl lertìlìzers are added Negative Eh va lues were never mea-sυred withcut the addi~' ion of fresh organic materi 剖, even after 18 weeks of f100dingIt is believed essential not to drain the soils at Carimagua once they are flocded and puddled , as draining would interrupt the reduction process and might 內心It in critical post-plant levels of soil-solution iron . Drainag.! would also result in soìl cracking and a partial 1055 of the effect of puddling Rice, therefore, should be seeded ;nt。 water , Pregerminated seed has been successfully seeded into 5-7 cm of water, and stands have generally been good Yields in a farm-size paddy of IR 8 grown during the second semester of 1970 ranged from 4,500 to 6,300 kgjha dry paddyThe Inter-American Center for Photointerpre'tation (CIAF) has provided a complete photo-mosaic and a ten'日 tiV2 soils map based on photo-jnterpretation of the 20,000-hectare Carimagua Center , The soils map will serve as the basis for locating a livestock management un 間 ìn a long-range research pro . Germin剖 ion was between 60 and 100 percent for all entries, bυt only 760 varieti es produced seed . The remaining 240 varieties produced some flowers but did not set seedThe following agronomic traits were scored for each entry: days to germination , days to f1o wering, days 10 matu-rI句, habit of growth and disea se reaction (rust , common b1i ght , mosaic , and powdery mildew). Disease reaction was scored from 0 to 5, where 0 was the absence of disease sym阱。 ms , and 5 was ccmplete susceptibility. Mosaic score was made without identifying the type of virus causing it. Habit of growth was classified as bush , v;ny or sem i-v iny 州。 re than 80 percent of the entries exhibited a disease reac'tion greater than 3, which means that more than 800 varieties showed susceptibilitγto one or more diseases.If the range of 0 to 1.5 is regarded as the range of res istance, the percent-aQe of disease resistance was as follows ru st, 17 percent; common bligh't, 10 percent; mosaic, 12 percent; and powdery mildew, 18 percentTraining and communic 剖昀n 咕。ne of the prolessiona 1 gr。υps 0 1 CIAT and consists of the following acti vities: Training, Informat ion Se rv ic肘, Field Proiects and Demo nstrations , Li brary, 5ta-:tistics , and Hou sing and F。咽d Services The Train1n9 and Co mmuni ca tion Program enCCm pÐSSeS acti v山出 dcsigned to achieve th;-ee primary 90315 considered instru menta l to the develop ment process \" To mobilize -the identilying 01 institution s and individuals whose roles in the various countrìes involved are highly re levant to thc development pro::ess 2 , To energize -the bringing t。 gether of these institut 的 ns and individuals 川 meaningful waγ5 and w ith a catalytic-type action. Th 問 川cludes information di ssemination , as well as conferenc肘, symposi a and other exchange activi'Ues 3. To qualify -the traíning 01 individuals so that they can eHec tively 1υnctíon in their own in sU tutions and thus enable th ose 。 rganizations to contribute sig nifi 臼 n'tly to the developm,nt processThese activities invol\\le a ba 剖 c philos 。phy 0 1 development which underlies CIAT' s edυcat ional programso It is con sidered that the central element 01 the development proc, ess is man him self , and therefore 'the behavio ral changes Ih 剖 cðn be effe~ted in order to improve the decîsio n-making abilities of individυals wi l1 bear upon the 叩開d with w hich the development process takes p lace 州。 re specifica l1 y ,的 is 'ta sk involves \" Helping people to know and un 回 derstond the 01個rnatives for ac t ion when f aced w 川、 a probl,m;2. Helping people 10 develop criteria for choos ing among a1ternate cou rses of action; and 3. Helping peo ple to acq山 re the capacity to anticipote the probable con 揖quences of the vari。υs alternati \\l es, and to u間 them as criteria for evaluating the appropri aO te ness of the ac tion take n in sol \\l 川 9 a particular problem In addition , a prime consider 肘 lon In all 01 CIAT' s trair、 ing activities is to inculcate in participating individuals a se nse of urgency and dedication t。 wo r k for the devel o pment of 'their count riesThe training programs begin with a d~ar definitic n of objecti\\les in behav . 咕咕 I change and level 01 performance The type 01 prolessional CIAT aims t。 produce is one wi ~h five major competencieso In addition to acquiring practical exper ience and applied training in farming w 川 hin the con'text 01 the hυ mid tropics ecology, this profess ional should also obt ain Irom CIAT's program a familiarity with and ability t。 handle the econom ic, scientific, tech-nìcaJ, and communication aspects of modern agricultural production and dif. Beginning in June, 1970, seven trùinees were selected from various universities in Colombia to p, articipate in a crop production specialist training course. After 15 days of intensive training in in'terviewing techniques and diagnosis of maize production problems , theγpartic ipated in a study to identily some of the factors associated with low ma 阻 e yi elds cbserved among small farmers in CoJombia. These traine2:s learned 'to ioteract effectively with the small farmer as they interviewed somc 3 日 o maize producers , both on the 39ro nomic as well as the socio-economic 笛一 pects of their operations. The training prog月 m for these crop production specialists is continuing through June, 1971 , with instruction in the production techniques of other crops grown under tropical conditions InvoJvement of these production specialists (whose oùckground is mainly of a techni日 I nature) in socio-economic research , as in the case of 'the study mentioned above, has proven to be an efficient mechanÎ5m both for instruc. t 的 n and for motivation of the trainee He becomes , thr。υgh this type 01 。仆 the-job training, directly involved w 川 h farmers and learns to analyze their problems from more than j ust a technical standpoint . The trainee thus ac. quires an understanding of the social context wi\"t hin which these problems 。ccur and comes in contact wi th the complexity involved În f 附ding appro. priate solυtlonsIn-service training is predicated on direct supervision by one or more staff members. Areas of sludy covered in 1 中 70 included a broad range 01 special. izations. Beef prodυctlon , swine production , and animal health were grouped under animal sôence ; and crop produc tion , lood legumes , plant pathology , opaque-2 maiz-e production , rice product 的 n and cassava production within plant science. The 25 trainees within this category were distributed as fo! lows: animal science, 5; plant science , 15; agricultural 缸酬。 mics , 3; and agricultural engineering, 2 Conferences and symp伯伯 help CIAT to establish closer contaCts with key institutions and pers。肘 , and 'co disseminate inform 叫咱 n and influence policy decision5 regarding important agricultural issues for the tropical countries Through it5 rice produc'tion program , CIAT has produced improved varieties with a higher-yielding capacity. These varieties are now ready to be released to rice-producing countries, and, as a related activity, a seminar on rice in Latìn America is scheduled for October [10][11][12][13][14]1971 Simultane。 υs translation equipment has been purchased for immediate use this year in CIAT's present lacilities and lor luture permanent installation in th含 projected Continυing Education Center bυildings In addi'tion to international activities regarding conferences and symposia , an internal seminar series WðS begun at CIAT headquarters in September, 1970, lor staff members and trainees . These seminars focus on agronomic and related 缸。 nomic problems within th3 low. iand tropics and present staff rr.embers and trainees with the opportunity to exchange ideas on research problems and their possible solutìons lated statements of 1ncome and expenses and of changes ln ~ld balances for the year. Our examinatlon was made 1n accordance wlth general1y accepted aud1t1ng standards and accord1ng1y 1nc1uded such 乞 ests of the accountlng records and such other audltlng prooedures as we cons1dered necessary 1n the clrcumstanoes. 1n our oplnlon , except for the understa 包 ement of 乞 he excess of expenses. over income described 1n the followl.ns paragraph J 乞 he acoompany1ng f1nanc1al statements exam1ned by us present fa1rly the f1nanc1a1 pos1t1on of Centr。工 nternac1ona1 de Agr1cu1tura Trop1cal (CIAT) 的 December 抖 , 1970 and the resu1ts of 1ts operatlons for the year J 1n conformlty wlth generally accepted account-1ng pr1nc1p1es.We stated 1n our opinion da 巳 ed May 自 , 1970 that_we regard the 1nclus1on 1n the f1nanc1a1 statem~nts for the year 1969 of cer-ta1n comm1tments for future expenses (ma1n1y materla1s .and\"supp11es) as not be1n在 10 accordance w1th generally accepted account1ng pr1nciples. These expenses were incuηed 1n 1970 and as a resu1t CIAT operates under an agreement signed with the Colombian govemment, the most Important stipulations of which are as follows 1. The agreement is for ten years but rnay be extended iC so desired by the partles thereto 2 CIAT is of a permanent nature and terminatìon of the agreement would not imply cessation of CIAT's e世 stence 3. I! CIAT ceases to exist. all of its assets wìll bc transferred to a Colombian edu. cational or other instiluUon considered approprlate by the parties to the agreement 4. CIAT is exempt from all taxes 5. CIAT is permitted 10 import. free of customs duties and other taxes. a>1 the eQuipment and materlaJs requlred for ils programs 6 . The government provides land for CIAT's purposes under a. rent9.1 contra.ct for ten years, aL a nominal rent τ\"tl is contract may be extended by mutual agreement NOTE 3In conformiLy with generally aC且 pted accOlUlting principles applicable to nonprofit organiz.ations. CIAT does noL record depreciation of its property and equipment NOTE' Granls are generally designated as to purpose (acquisition o( capital assets or payme叫 。 C expenses ). When no such specification is made the part of the funds received which Is used to purchase capital as扭扭 is included in the c8pital 8sset fund and the remainder in income of the operating fund","tokenCount":"16533"} \ No newline at end of file diff --git a/data/part_6/1240751135.json b/data/part_6/1240751135.json new file mode 100644 index 0000000000000000000000000000000000000000..bbabd458985b84b2ffe9a6ac29c15980d39a37cf --- /dev/null +++ b/data/part_6/1240751135.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"3b4baa11e37de276b1852891bfef5be6","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/3da80000-0848-4c3e-b995-9c9291e5087a/retrieve","id":"-1463773288"},"keywords":["Climate change adaptation","breeding","crop modelling","environmental characterisation"],"sieverID":"d2c1de5d-a7b0-44a2-bed6-1803ccec8503","pagecount":"28","content":"Environmental characterisation to guide breeding decisions in a changing climate. CCAFS Working Paper no. 144. CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS). Copenhagen, Denmark.Julian Ramirez-Villegas works as a researcher on climate impacts at the School of Earth and Environment, University of Leeds, UK, and is affiliated with the Climate-Smart Agriculture Flagship of the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), and the International Center for Tropical Agriculture (CIAT). Julian can be contacted by email at: j.r.villegas@cgiar.org or J.Ramirez-Villegas@leeds.ac.uk.Alexandre B. Heinemann works as a researcher in ecophysiology at the Rice and Beans unit, at the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa), Brazil, where he leads research on physiology of rice and common beans.!Alexandre can be contacted by email at: alexandre.heinemann@embrapa.br.Agriculture faces and will continue to face multiple challenges. Most notably, the need to meet food demand for a rapidly growing and urbanising population under increasingly variable and warmer climates (Wheeler and von Braun 2013;Tilman and Clark 2014).Substantial evidence now exists suggesting that agricultural yields will have to increase significantly in order to meet food needs during the 21 st century (Ray et al. 2013;van Oort et al. 2015). One such way of increasing yields is to develop high yielding cultivars through crop improvement (Chapman et al. 2012;Dingkuhn et al. 2015). Additionally, the development of novel, climate-adapted varieties that ably tolerate stresses will be key in order to respond to regional climatic changes (Asseng et al. 2014;Ramirez-Villegas et al. 2015), particularly if no mitigation policies are enforced (Müller et al. 2015).The development of high yielding and climate-adapted crop varieties, however, requires an understanding of how crops respond to spatio-temporal variations in soil, climate and management, as well as an assessment of the main factors limiting yields. This is because genotype-by-environment interactions sometimes prevent plant breeding progress for broad adaptation and/or for adaptation to specific conditions within a region (Chenu et al. 2011).Therefore, understanding yield constraints and their spatio-temporal variations will ultimately lead to improved priority setting and more rapid progress in breeding programs. This Working Paper summarises the results of a CCAFS project named Target Population of Environments (TPE). The project aimed at providing actionable information to crop breeders and, therefore, inform breeding decisions. Our method focuses on classifying growing environments and stress patterns, using a combination of controlled field trials and crop simulation models driven by observed soil, climate and management data (Sect. 2). We also present the application of the method on two crops in Brazil (Sect. 3), as well as a web-based tool for visualisation of results (Sect. 4). We conclude by setting out potential avenues for future research (Sect. 5).As stated earlier, our method combines field experiments, observations of environment (climate, soils) and management, and crop simulation models to develop a classification of environments and stress patterns. These classifications are then quantitatively assessed against the current breeding pipelines for the regions under study in order to assess the potential impact of stress-tailored breeding strategies. Fig. 1 presents an overview of the process. The methodology consists of seven steps, as follows:1.! The first step is to define the study region and crop under study and the crop model to be used, and to collate experimental data for model calibration and evaluation.Because breeding is a highly crop-and region-specific discipline, the area and crop under study are straightforward choices in most cases. The crop model, however, needs to be defined with care. Particular attention has to be paid to whether a wellestablished model has already been calibrated and evaluated for the study region [e.g. Lobell et al. (2015)], or whether the model of choice is suitable for the conditions in the study region. The selected crop model needs to be able to simulate processes that are relevant to the region. In some cases, it may be desirable to use more than one crop model in order to assess crop model uncertainty (Asseng et al. 2013). Gathering experimental data is also key to the success of this approach. Trial data should include relevant varieties in the region, should ideally be multi-location and/or multi-year, and should include both potential yield and stress-induced yield trials. Field data should be as detailed as possible, including time-varying measures of multiple plant attributes (e.g. leaf area index, organ-specific biomass), as well as weather, soils and management inputs.2.! Once the crop model has been defined and the experimental data has been collected the next step is to calibrate the crop model using a set of field experiments. To this aim, the experimental data should first be thoroughly checked for possible errors and then split into a `calibration` set and an `evaluation` set. The objective of model calibration is to adjust influential model parameters within their reasonable ranges so that modelling results are comparable to observed data (Wallach et al. 2014). Multiple methods exist to derive model parameter values for crop models (Angulo et al. 2013;Wallach et al. 2014;Alderman et al. 2015), and some of these are already built-in and well-tested for certain crop models. However, regardless of the method, it is key to ensure that the parameter values fall within plausible ranges and represent the morpho-physiological attributes of the varieties being parameterised. 3.! Simulations are then run per crop variety and assessed against the evaluation data.The primary aim of this step is to ensure that the model is capable of reproducing an independent set of observations. The result should be a parameter set (per variety) that can later be used to run spatially-explicit and time-varying simulations for the study region. Evaluation metrics often used in crop modelling to measure the distance between measured and simulated values include the Root Mean Square Error (RMSE), the RMSE relative to the mean or the standard deviation (RRMSE), the mean absolute error (MAE), the correlation coefficient (R), and the Wilmott d-statistic (Willmott et al. 2012). Model performance can also be assessed both numerically and visually through the Taylor diagram (Taylor 2001).4.! The fourth step in this process is to run spatially explicit crop model simulations for the study area (across k sites), for a representative period of n years and for a number of management scenarios (m). For this, either high-resolution gridded daily weather or daily weather station records of a representative number of weather stations are needed. Weather variables needed for crop simulation are: minimum and maximum temperatures, downwards shortwave solar radiation, and precipitation. Soil profile data (i.e. lower, upper and saturation moisture contents) for all locations where the crop model is to be run are also needed. Management scenarios are constructed as a combination of planting dates, planting densities, and/or fertiliser application regimes (Heinemann et al. 2015a;Lobell et al. 2015). Model runs are finally performed for each of the site*year*management situations. 5.! Once simulations are completed, environmental groups (EGs) need to be determined.To this aim, statistical clustering is performed on the simulated crop yields of all the site*year*management scenarios. Clustering by yield helps separate situations of low and high yields, without necessarily assessing their causes -which will be assessed in step 6. Various clustering methods exist, including the hierarchical clustering used by Heinemann et al. (2015a) in Brazil, the k-means clustering used by Harrison et al. (2014), or more complex neural-network-based methods (Reymondin et al. 2012).Clustering efficiency and stability indicators are then used to determine the optimal number of EGs for the study region. However, it is also important to take into account the expert knowledge of regional breeders and/or agronomists to define the number of EGs. Using the results of this first clustering, maps of EG distribution and frequency can be produced.6.! With an understanding of which site*year*management combinations belong to the different EGs, the next step is to determine the main stresses for each group, i.e. stress profiles. Because this step requires statistical clustering of stress-related modelled variables (i.e. stress index), an a-priori idea of stresses in the region should inform this step. For example, the ratio of actual to potential evapotranspiration is usually a good indicator of water stress (Heinemann et al. 2015a). Similarly, the fractional reduction in grain-set from high temperature can help differentiate heat stressed and non-heat stressed situations (Lobell et al. 2015). For nitrogen or phosphorous stress, the ratio of uptake-to-required nutrients could be used. Clustering is performed individually per EG using the seasonal variation of the relevant modelled stress index.Step 5, a number of clustering algorithms can be used to perform this classification.7.! The final step is to use the environmental groupings and the stress profiles to calculate how much additional area in the study region can be covered if the breeding strategy is extended to include stresses that are not currently considered. This step is expected to make a clear case for, for example, the inclusion of additional sites for germplasm selection under specific conditions.Having described our methodology, we now present two case studies: one for upland rice in central Brazil (states of Goiás, Tocantins, Mato Grosso and Rondônia) and another for common beans in the state of Goiás in Brazil. We provide a summary of key results and conclusions. For more detailed descriptions the reader is referred to Heinemann et al. (2015a) for upland rice and Heinemann et al. (2015b) for common beans.Upland rice (UR) is a key part of the central and northern Brazilian diet, and is the main source of income for many smallholders in the savannah region. The current UR growing area is, however, half of what it was 10 years ago. To an unknown but likely significant extent, these reductions in UR growing area in central Brazil are a product of the UR breeding program strategy, whereby direct grain yield selection is performed primarily under optimal growing conditions. Recent evidence, however, suggests that drought stress conditions are prevalent across central Brazil (Heinemann and Sentelhas 2011), and hence limit the efficiency of the UR breeding program. Here, we hypothesise that the impact of the UR breeding program can be enhanced by better accounting for drought conditions across the UR growing region.The analysis region comprises the states of Goiás, Tocantins, Mato Grosso and Rondônia (Fig. 2). We gathered data from 17 different experiments conducted at the Embrapa Rice & Beans experimental station in Santo Antonio de Goiás (GO). Six of these experiments were used for calibrating phenology and growth parameters, and the remaining 11 were used for model evaluation. Model calibration experiments included measurements of dates of emergence, flowering, and physiological maturity, as well as of leaf area index and stem, leaf and panicle biomass. Evaluation experiments included only dates of flowering and maturity, and crop yield. We used the crop model Oryza2000 as it has proven to simulate rice yields accurately across a wide range of environmental and management conditions (Bouman and van Laar 2006;Li et al. 2013). Calibration of the model was performed using the built-in genetic algorithm of the Oryza2000 model for the cultivar BRS Primavera -a representative check cultivar for the four states under analysis. For model evaluation, we computed the Spatially-explicit simulations were conducted for 51 weather station zones (defined using Thiessen polygons), 7 soil types (defined based on texture), 8 sowing dates (defined at 10-day intervals from 1 st Nov. to 10 th Jan.), and 33 years , for cv. BRS Primavera. We then classified the simulated yields using a hierarchical clustering method (Ward 1963), and determined the optimal number of groups using the inertia gain, the within-groups sum of squares, and expert knowledge. Results indicate the existence of three EGs: a highly favourable environment (HFE), a favourable environment (FE), and a least favourable environment (LFE) (Fig. 2). The HFE showed mean yields of 3,168 kg ha -1 , and represented 19 % of the production region; the FE presented a mean yield of 2,610 kg ha -1 and represented 44 % of the simulated scenarios; and, finally, the LFE showed the lowest yield (1,661 kg ha -1 ) and represented 37 % of the seasons. The occurrence of HFE was associated with clay soils and early planting dates, whereas the occurrence of LFE was often associated with sandy loam and sandy soils and late planting dates.Based on our knowledge of the study region, we defined the stress index as the ratio between actual to potential evapotranspiration. In the Oryza2000 model, this factor is used to reduce photosynthesis, and hence is a suitable indicator of drought effects on biomass accumulation and growth dynamics. For each EG we clustered weekly variations in the stress index using the same hierarchical clustering method as that used for determining the EGs. Results indicate the existence of 2 stress patterns for HFE, and 3 stress patterns for both FE and LFE (Fig. 3).For HFE, the two stress patterns correspond to stress-free conditions (69 % occurrence in this EG, profile 1 in Fig. 3) and to terminal drought stress (31 % occurrence in this EG, profile 2 in Fig. 3). For FE, the stress profiles are: [1] reproductive stress (41 % occurrence in this EG); •! In the best environment (HFE, 19 % occurrence), the current strategy is likely to perform well. In Santo Antônio de Goiás (GO) (red flagged point in Fig. 2), where early generation yield testing is performed, HFE shows 62.5% probability of occurrence, suggesting that this site is suitable for selecting for potential yield. Sowing should be undertaken at the beginning of November and in clay soil. Irrigation, however, may be needed in order to avoid stress in certain years.•! In the favourable environment (FE, 44 % occurrence), two distinct stress patterns occur roughly four in every five cropping seasons. One option is to perform selection for wide adaptation to drought, as suggested by Chenu et al. (2011). Another option is to weight the performance of genotypes according to the representativeness of the growing environments where they are tested (i.e. weighted selection).•! For the least favourable environment (LFE, 37 % occurrence), we suggest selection to be specific to high yield under reproductive stress, which is the most likely stress profile in this environment (68 %).Based on our analyses, we estimate that an additional 42% of coverage (from total) would be possible if breeders were to broaden their selection strategy to select for reproductive stress (which corresponds to 41 and 68% within LFE and FE,respectively). This amounts to a fourfold increase in the coverage of the UR breeding program, which we argue is likely to be cost effective. Realising this potential, however, will ultimately depend on the existence and efficiency of seed systems, and on the adoption barriers to new germplasm.Brazil is the largest producer and consumer of common bean (Phaseolus vulgaris L.) (FAO 2014). In the state of Goiás (a large bean producer in Brazil), crop production occurs in three growing seasons or `safras`. A first season (wet season), for which sowing occurs between 1 st Nov. and 31 st Dec.; a second season (dry season), for which sowing occurs between 1 st Jan. and 28 th Feb.; and a third season (winter season) for which sowing occurs from 1 st May to 30 th Jun. Both the dry and wet seasons are rainfed, whereas the winter season is irrigated. The difference in planting times and yields between seasons implies the occurrence of different types of stress across time and space. Major limitations in the wet and dry seasons are low soil fertility, drought, nitrogen deficiency due to poor nitrogen fixation, as well as several bacterial, fungal and viral diseases (Beebe et al. 2011;Souza et al. 2013;Araújo et al. 2015).The Brazilian common bean breeding program, led by Embrapa's Rice and Beans unit, focuses on developing germplasm with broad adaptation for all Brazilian bean production regions. In their scheme, the early generation screening yield trials (nursery) are always performed in the winter season under well-watered conditions (i.e. fully irrigated). Although this is a cost-effective strategy, the main caveat is that it increases the risk of developing genotypes that do not respond well under stress. We analyse drought stress patterns in the rainfed (wet and dry) seasons in order to determine the extent to which the current strategy of the common bean breeding program needs to be adjusted to include drought stress conditions.As stated above, we concentrate on the state of Goiás (Fig. 4), for both wet and dry seasons.We assembled a large database of field experiments (41 in total) for two cultivars: cv. Pérola for Agrotechnology Transfer version 4.5 (Jones et al. 2003). Model calibration was performed following Alderman et al. (2015), who adopt a Markov Chain Monte Carlo approach and the Metropolis Hastings algorithm for parameter estimation. The CSM-CROPGRO-DRYBEAN model showed acceptable performance for both cultivars in simulating end of season yield and phenology, with most simulated quantities within a 95 % confidence interval derived from observations. The model also captured well the seasonal variations in dry matter dynamics, leaf area index and soil moisture under moderate drought, but was unable to adequately simulate severe drought conditions. To our knowledge, however, severe drought only rarely occurs in the wet and dry seasons. Multi-environment simulations also showed good agreement with observations, with RMSE (MAE) values for Radiante and Pérola being 404 (328) and 322 (319) kg ha -1 , respectively.Simulations were conducted for the period 1980-2013 for 26 weather station regions (defined using Thiessen polygons), for 3 soil types (oxisol, ultisol, inceptisol), and for a total of 13 sowing dates (defined at 10-d intervals from 1 st Nov to 30 th Dec for the wet season, and from 10 th Jan to 28 th Feb for the dry season) for the two cultivars (Pérola and BRS Radiante).Clustering of yields and determination of the number of EGs was undertaken as it was for upland rice. Fig. 4 shows the EGs for both seasons.For the wet season, results indicate the existence of two markedly different EGs: a highly favourable environment (HFEw, 44 % occurrence) and a favourable environment (FEw, 56 % occurrence) (Fig. 4, top maps). The HFEw has an average simulated yield of 3,655 kg ha -1 , whereas the FEw has an average simulated yield of 2,870 kg ha -1 . For the dry season (Fig. 4, bottom maps), we also find two EGs: highly favourable environment (HFEd, 58 % occurrence) and favourable environment (FEd, 42 % occurrence). Yields in HFEd were For the wet season, two stress profiles exist for the highly favourable environment (HFEw).Namely, stress-free (pattern 1 in top panel of Fig. 5, 86-88 % occurrence depending onWe developed a web application to visualise the results of the application of this approach to different crops and regions: http://www.ccafs-tpe.org (Fig. 6). The tool consists of three main components:•! A map application to visualise spatially explicit environmental groups, to query daily weather data and soil data, as well as to visualise dynamic graphs of yield variation and of stress profiles.•! Case studies and applications providing summaries and key findings of the analyses we have conducted so far. These include: upland rice in Brazil, dry bean in central Brazil, and irrigated rice in Colombia. Additionally, we blog about papers that use methods akin to ours.•! Documentation and data downloads: we provide detailed information on our methodology and results of our case studies, including free versions of published journal articles, reports and tutorials. We also allow users to download our project input and output data. As part of the CGIAR Research Program on Climate Change, Agriculture and Food Security (CCAFS), we implemented a project named Target Population of Environments (TPE). We developed a methodology to classify environments and to determine stress profiles within these environments. Our results for upland rice in central Brazil suggest that breeding should be adjusted to include selection under drought conditions. On the other hand, results for common bean in the state of Goiás (Brazil) suggest that drought stress does not occur with enough frequency so as to warrant selection under drought conditions. However, for beans, we note that differences in observed farmer yields between seasons (wet, dry and winter)suggest that there may be other stresses acting to reduce yields. Future work should focus on identifying and characterising such stresses.The priorities identified by our analyses correspond to current climates, and, while they serve to identify clear priorities where breeding gains are necessary presently, climate change may imply shifts in some of these priorities [e.g. Lobell et al. (2015)]. As an obvious next step in this project, we are conducting analyses for upland rice and common beans in Brazil where we quantify changes in stress patterns under future climates. We are also extending the analyses to rice in sub-Saharan Africa, and flooded rice in southern Brazil.Finally, we wish to remark that other methods exist that classify environments for breeding.Many of these have been successful to orient crop improvement strategies in the last two or three decades (Hodson et al. 2002;Ortiz et al. 2008;Cairns et al. 2013). Future work should also focus on identifying how different approaches to environmental grouping can be used together to improve breeding practice.","tokenCount":"3480"} \ No newline at end of file diff --git a/data/part_6/1279640867.json b/data/part_6/1279640867.json new file mode 100644 index 0000000000000000000000000000000000000000..712e00bec80b0043111dbf16b7786643d4d7aff5 --- /dev/null +++ b/data/part_6/1279640867.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"7ce15634f947b22de1d3f3e65c91f4d3","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/0d4c4965-89c4-439a-a46f-d8412b675993/retrieve","id":"1324284486"},"keywords":[],"sieverID":"f377b237-4cc1-4425-b3ae-0675a4793b22","pagecount":"45","content":"En los últimos años se ha llegado a un consenso mundial acerca de la necesidad de un desarrollo sostenible. Además de un cambio de perspectiva acerca del desarrollo, el concepto del desarrollo sostenible implica nuevas demandas. Una de éstas es la producción de información, la cual debe jugar un papel critico, puesto que ella puede proveer de bases firmes al proceso de toma de decisiones y al seguimiento del desarrollo. Para responder a estas demandas, en 1995, el CIA T y el PNUMA iniciaron un acuerdo de cooperación con el fin de elaborar indicadores para el monitoreo del desarrollo y el medio ambiente en América Latina y el Caribe como herramienta de apoyo a la toma de decisiones y a la planificación. Como resultado de dicha cooperación se inicio el proyecto de Indicadores Ambientales y de Sustentabilidad: Una Visión para América Latina y el Caribe, con el objetivo de elaborar un producto, en forma de Disco Compacto, que permita:El Atlas CD contiene los siguientes directorios: X:\\ X: \\data \\docu X: \\data \\imgdata X: \\data \\mapas X : \\data \\mode~o X: \\data\\ tab~es X: \\program este directorio contiene setup. exe este directorio contiene toda la documentación este directorio contiene las imágenes este directorio (y los subdirectorios) contiene datos sobre vectores este directorio contiene el modelo de uso de la tierra este directorio (y los subdirectorios) contiene cuadros.dbf este directorio (y los subdirectorios) contiene el programa ArcView Data Publisher donde X es la letra del dispositivo para el CD-ROM.Para instalar el Atlas CD, ejecute setup. exe desde el directorio raíz del CD-ROM. Esta operación iniciará el proceso de instalación. El programa preguntará el lugar del disco duro en que usted quiere instalar el Atlas CD. Usted podrá elegir también entre instalar la base de datos (DATA) en su computadora o dejarla en el CD-ROM.Nota: El Atlas CD está disel'iado para tener acceso a toda la información desde el disco compacto. Sin embargo, usted puede trasladar los datos a un lugar más conveniente, especialmente si hay usuarios que no tienen acceso a una lectora de disco compacto. Si Usted escoge instalar la base de datos en su computadora es necesario disponer de 500 megabytes libres en su disco duro.Instalación del modelo de simulación de uso de la tierra Ejecute el archivo X : \\data \\mode~o \\insta~~ \\setup. exe.Este paso inicializa el programa de instalación del modelo. Se le solicita luego escoger un directorio en el cual instalar el programa Escoja el directorio por defecto. La rutina de instalación copiará entonces Landuse . exe en el directorio especificado; también copiará cierto número de archivos en su directorio de WINOOWS\\SYSTEM. Estos archivos permiten ejecutar el modelo.Este proceso debe repetirse en cada computadora en que se ejecutará el Atlas CD.Hay varias formas de iniciar el Atlas CD: Cuando usted instale el Atlas CD, se creará un grupo de programas y un ítem en Windows en su computadora personal. Dar doble click en el icono Atlas CD para iniciar la aplicación.Una forma alterna es, situándose en el Manejador de Archivos, dar doble click en el icono unepcia t. ex e del directorio X : \\ATLASCD\\bin.Nota: Atlas CD puede no funcionar correctamente si Usted tiene instalado Are view 3.0. Si Usted encuentra algún problema por favor visite nuestra pagina en Internet o contáctenos (ver pagina 40)Al abrir el proyecto, se le solicitará indicar la unidad de disco en la cual se almacenarán los datos. Toda la información debe encontrarse en una Alias de md1CaDores amb1entales y de suste ntab1hdad misma unidad de disco y debe mantenerse la estructura del directorio original; de otro modo, los nombres de las vlas de acceso serán incorrectos y los mapas no se cargarán en forma apropiada.¿fn cuál unidad de disco almacena los datos ? (On which drive are the data stored ?):le lí!1Atlas de md1CaDores ambientales y de sustentabrhdad También se le soliE:itará indicar el tamafto de la pantalla del monitor en que usted está visualizando los datos. Esta información es necesaria para que aparezca la escala correcta del mapa en la pantalla. Se le ofrecerán luego cinco opciones, que varlan desde una computadora portátil hasta una pantalla grande. Si usted no está seguro del tamafto de su Elige la dimensión de la pantalla (Choose your screen size}:Grande [large 20 inch)Scripts pantalla, o si ninguna de las opciones es apropiada, entonces haga clic en 'Otro'. Se le pedirá, entonces, que indique la anchura y la altura de la pantalla, en ese orden.El proyecto se carga ahora y aparece en pantalla la ventana del proyecto unepciat.apr. Esta ventana le mostrará las vistas que presenta el proyecto.Hay sólo una vista llamada ' Atlas CD' , que se abre automáticamente cuando usted accesa el proyecto.Cuando usted abre la vista, notará que cambian los menúes y aparecen más iconos en la barra de herramientas.Los seis primeros menúes son los convencionales de ArcView.Los siguientes siete menúes se relacionan únicamente con el Atlas CD.Estos menúes son: Estos serán, probablemente, los iconos más usados:Este icono elimina de la vista todos los temas activos.Este icono muestra la fuente, la fecha y el tipo de información de todos los temas activos.Este icono amplia hasta donde se extiendan todos los temas.Este icono amplia hasta donde se extiendan todos los temas activos.Este icono amplia hasta donde se extiendan cualquier característica elegida.Este icono activa la caja de diálogo de construcción de una consulta.Los ' temas activos' son aquellos indicadores que se eligen (es decir, aparecen resaltados) en la tabla de contenido de la vista.Estas serán, probablemente, las herramientas más usadas:Haga clic en este icono cuando qu iera identificar una característica en cualquiera de los temas activos. Esta herramienta modifica el cursor y le permite escoger una característica. Cuando usted haga clic en una característica, los atributos aparecerán en un cuadro.Haga clic en este icono cuando desee seleccionar características en los temas activos. Esta herramienta modifica el cursor y le permite a usted hacer clic en la característica o crear una caja de selección, haciendo clic sostenido y arrastrándola con el ratón. Cuando haya seleccionado las caracterlsticas del mapa, éstas se tomarán amarillas.Haga clic en este icono cuando desea un acercamiento del área de la vista en pantalla. Usted puede hacer clic una vez en el mapa para ir acercando (por un factor de 2), o hacer clic sostenido y arrastrar con el ratón para crear una nueva extensión.Haga clic en este icono cuando desee un alejamiento (visualización más distante) del área de la vista. Usted puede hacer clic una vez para ir alejando (por un factor de 2), o hacer clic sostenido y arrastrar con el ratón.Haga clic en este icono cuando desee desplazarse por el mapa cuando éste es demasiado ancho y no se ajusta por completo a la pantalla. Esta herramienta modifica el cursor y le permite a usted mover el mapa en la dirección del cursor. Si hace clic sostenido en el cursor y arrastra con el ratón, verá que el mapa se mueve con el cursor.Haga clic en este icono cuando quiera mirar las gráficas relacionadas con algunos indicadores. Esta es la herramienta de enlace; al hacer clic en el símbolo apropiado del mapa, usted puede ver la gráfica.Haga clic en este icono cuando quiera seleccionar formas o gráficos. Esta herramienta modifica el cursor y le permite elegir una forma. Sin embargo, no puede usted seleccionar las características de cualquiera de los indicadores, sino sólo las formas que usted ha dibujado.Haga clic en Guia de Uso. Aparecerá un menú desplegable. Este menú desplegable presenta dos opciones:Haga clic en la opción Programa para abrir la guía que está leyendo ahora.Haga clic en la opción Indicadores para abrir la gufa que contiene información sobre indicadores ambientales y de sostenibilidad.Introducción Esta sección contiene varios temas relacionados con los antecedentes del proyecto, donde encontrara el propósito y la definición del marco conceptual de referencia.Esta sección contiene análisis, cuadros y figuras para todas las variables de los indicadores en los menúes desplegables. Cada sección destaca los indicadores más importantes y debería ser una gufa útil para quienes diseñan la política. La sección contiene también notas técnicas que explican la forma en que se han creado Jos diversos mapas.El glosario defme muchos de los términos que se usan en el texto o que describen los indicadores.Cada sección contiene muchos enlaces de hipertexto. Los enlaces en verde lo llevan a otro tema de ayuda. Los que están en azul abren figuras relevantes y los de' color dorado abren cuadros. Hay también enlaces resaltados en rojo. Basta con hacer clic en el hipertexto, y se cargará un mapa en la ventana de visualización del At las CD. Usted puede regresar al Atlas CD en cualquier momento. Basta con cerrar la gula o dejarla abierto haciendo clic en la vt:ntana Atlas CD.Haga clic en Datos Básicos, en la parte superior de la pantalla. Aparecerá un menú desplegable. Este menú desplegable presenta JI opciones. Para visualizar cualquiera de estos temas, haga clic en el nombre respectivo de la tabla a la derecha. El mapa correspondiente al tema aparece al lado derecho de la vista, y la leyenda respectiva aparece a la izquierda en la tabla de contenido. El orden en que los mapas se exhiben en la sección de visualización de la vista está determinado por la lista de temas que aparece en la sección de la tabla de contenido de esa vista. Los temas que aparecen al fm de la lista se dibujan primero, y los que están en la parte superior aparecen de último.Para alterar el orden de los mapas, seleccione el tema o los temas que desea usted cambiar haciendo clic en la tabla de contenido. Haga un clic sostenido sobre el título del tema (o de los temas) y llévelo, arrastrando el ratón, hasta una nueva posición en la tabla de contenido. Los mapas aparecerán automáticamente según la nueva orden.Las imágenes se usan profusamente en todo el Atlas CD, pero hay varias cosas que usted debe tener presentes cuando las utilice.No es posible ver a través de una imagen. Por lo tanto, una imagen debe usarse principalmente como fondo. Es posible hacer que algunas partes de la imagen sean transparentes, pero los colores mismos siempre son sólidos. Por regla general, la mayoría de las imágenes se hicieron tan transparentes como fue posible para ev itar estos inconvenientes.Cuando se cargan, las imágenes se trasladan (por defecto) hacia el fin de la tabla de contenido. Esto significa que la imagen aparecerá primero y luego vendrán otros indicadores.Normalmente, no hay leyendas asociadas con las imágenes, a diferencia d. e los temas de tipo vector. Sin embargo, todos los indicadores que se visualicen mediante imágenes tienen también otro tema de ' leyenda' que se carga al mismo tiempo. Este tema de ' leyenda' aparecerá debajo de la imagen misma en La tabla de contenido.Una de las funciones más útiles que cumplirá el paquete es la superposición de diversos mapas. Este proceso es muy engorroso si se hace manualmente, pero es increfblemente sencillo cuando se usa el SIG. Los usuarios cargan sencillamente un tema después del otro y luego eligen el que debe aparecer encima de los otros. La superposición de mapas permite que los usuarios realicen análisis visuales sencillos. El análisis puede mejorarse mediante el acercamiento o visualización más cercana del área de interés.Si usted desea ver infonnación más explicita acerca de una característica especlfiéa del mapa (punto, linea o área), debe activar primero el tema del mapa haciendo clic en el nombre respectivo en la tabla de contenido.Después debe hacer clic en la herramienta Info 1 OJ de la barra de herramientas, y luego clic en la característica del mapa. De esta manera, traerá la entrada correspondiente a esa característica al cuadro de atributos asociados (ver el tema de ayuda en Hnea de ArcView 'ldentifying features on a view' [Identificando características de una vista] para detalles adicionales).Hay varias maneras de seleccionar características de una vista. Usted puede elegirlas según su ubicación o sus valores de atributo, o seleccionando sus registros en un cuadro. Usted puede seleccionar características de un tema o de varios. Para poder seleccionar las características de un tema, usted debe hacer clic en el tema en la tabla de contenido para activarlo.Las características se toman amarillas en la vista en pantalla cuando se seleccionan y permanecen resaltadas hasta que se hace una elección diferente o se cancela la elección original.Utilice la herramienta 'select Feature' l~bJ para elegir características en el mapa.Selección de una característica utilizando la herramienta 'Find' 'Find' (encuentre) es una forma rápida de seleccionar una característica específica de interés. Por ejemplo, si usted está trabajando con un indicador de ciudades grandes y quiere seleccionar ' Buenos Aires', sencillamente haga clic en el icono 'Find' 1\" 1 y escriba ese nombre en la caja de diálogo. El programa buscará los atributos de todas las ciudades para ver si el nombre aparece. La primera caracterfstica que lleve ese nombre será seleccionada y la visualización en pantalla se centrará en la localidad que tenga esa característica. Si esta no es la característica correcta, repita la secuencia y el programa encontrará la próxima característica que lleve ese nombre .Use la opción de consulta (ver pagina 16) cuando usted quiera seleccionar caracterlsticas según sus valores de atributo. Por ejemplo, usted puede hacer una consulta seleccionando todos los estados de Brasil cuya población sea mayor que 1 O millones de habitantes.La mayorla de los indicadores del paquete tienen un cuadro de atributos relacionado con el mapa. El valor de los atributos defme el color y la textura del mapa. Usted puede seleccionar caracterlsticas en el mapa abriendo el cuadro de atributos y seleccionando los registros. Cuando han sido seleccionados, los registros del cuadro se tornan amarillos y las características del mapa también se tornan amarillas. Para abrir el cuadro, debe asegurarse primero de que el indicador está activado en la tabla de contenido. Después debe hacer clic en el icono 'Tables' (cuadros)~-Verá entonces el cuadro de atributos del indicador.Cuando usted hace clic para seleccionar un registro del cuadro, el regisiueda resaltado; si usted regresa a la vista del mapa y hace clic en el icono 'Zoom to Selected Features' ~ , el mapa presentará un acercamiento hasta el nivel de cualquier característica que usted acabe de seleccionar. (Para más detalles, consulte el tópico de ayuda en línea de ArcView 'selecting features on a view' [Selección de características de una vista]).El Atlas CD permite a los usuarios hacer consultas más avanzadas a la información que posee si se hacen interactuar los diferentes indicadores de diversa manera.E\\ ejemplo que se presenta a continuación le indicará la forma en que usted puede seleccionar todos los centros de producción de frijol que queden a 5 Km de determinados caminos en una red vial.Cargue primero el indicador red vial y el indicador centros de producción de frijoles: Visualización de la fuente de información ' \"\\.. . \\,.. , Casi todos los indicadores tienen infonnación sobre la fuente. Esta infonnación incluye el nombre de la fuente, la fecha de la fuente y el tipo de datos.Para visualizar la infonnación sobre la fuente:Active primero el tema del mapa haciendo clic en ese nombre en la tabla de contenido .Luego hará clic en el icono 'Examine'(~ r situado en la barra de herramientas .Esta operación hace aparecer una caja de diálogo de infonnación que contiene la fuente, la fecha y el tipo de datos del indicador.Haga clic en OK para avanzar al siguiente tema (si usted ha activado más de un tema) o finalice .Acceso al modeloHaga clic en el menú titulado Modelo de Simulación .Aparecerá un menú desp legable. Este menú desplegable sólo presenta una opción: Modelo de Simulación del Uso de Tierras.La primera ventana que se abre da una breve introducción sobre los modelos de uso de la tierra.En la parte inferior de la ventana se le pide escoger un escenario.Se escoge un escenario haciendo clic en el botón contiguo al nombre. Hay dos posibilidades, Convencional (CDS) o Sostenible (SOS).Después de haber escogido un escenario, usted puede mirar las hipótesis en que se apoya ese escenario.Haga clic en el botón Mira Hipótesis. Se abre entonces otra ventana que detalla la hipótesis.Para regresar a la ventana de introducción, haga clic en el botón Regresa.Para abrir la siguiente ventana, haga clic en Próxima Etapa .La siguiente ventana se titula Zonas de Vida. Aqul es donde usted escoge la 'zona de vida' en que aplicará el modelo.Hay 18 opciones de zona de vida: • Para escoger una zona de vida, basta con hacer clic en el icono radiado a la izquierda del código de la zona de vida.Al escoger la zona de vida, se ejecuta el modelo. Se puede entonces visualizar los gráficos del uso proyectado de la tierra, leer el análisis de los cambios proyectados, regresar para cambiar el escenario o cerrar el programa.Visualización de los gráficos [ \"\"':..,M\"\"'\"''\"\"' 1 • Cambios en Uso de Tierra • Deforestación • Tierra Agrícola per capitaSi usted escoge Deforestación o Tierra Agrícola per capita, el gráfico se presentará inmediatamente en la pantalla. Sin embargo, si usted escoge Cambios en Uso de Tierra, tendrá que escoger, además, los tipos de uso de la tierra que quiera visualizar en el gráfico. Estos tipos de uso de la tierra pueden escogerse de la caja titulada Uso de Tierra situada en la esquina superior derecha de la ventana. Hay siete tipos de uso de la tierra:Haga clic en el icono situado a la izquierda de cualquiera de los tipos de uso de la tierra que desee visualizar.Luego haga clic en el botón Dibuja .Los cambios proyectados de uso de la tierra aparecerán en el gráfico.En la caja de Estado, aparecen:• Superficie en Uso • Superficie de Bosques Para usar el indicador Superficie en Uso -tal como se hizo en Cambios en Uso de Tierradeben escogerse los tipos de uso de la tierra que quiera visualizar en el gráfico.En la caja de Impacto/Efecto, aparece:• Tierra Alterada l .. \"\":'•\" ~rr::OOCUMEt\\ 1 ~~ IUH En la caja de Respuesta, aparecen:Los gráficos proporcionan un análisis visual muy bueno de las proyecciones de los modelos. Si usted desea mayores detalles o una interpretación de los resultados, puede abrir la ventana de Análisis.Para abrir esta ventana, haga clic en el botón Análisis de la ventana del gráfico o de la ventana de la zona de vida.La ventana de Análisis se divide en dos: un conjunto de cuadros y un texto. Los cuadros resumen los indicadores presentados en los gráficos. Hay también una interpretación de ambos escenarios, que destaca los resultados más importantes. Query Builder (constructor de consultas) está también disponible en la caja de diálogo de Theme Properties (propiedades del tema), donde se usa para definir la selección de una caracterlstica para un tema.Para construir una consulta, elija un Field (campo), luego un Operator (operador) y luego un Value (valor). Se formula una consulta al hacer doble clic sobre estas opciones con el ratón o al escribirla directamente en la caja de texto de la consulta. Por defecto, la consulta se enmarca entre paréntesis, aunque es posible, según la complejidad de su consulta, que no se requieran los paréntesis. Si la opción de Update Values (actualizar valores) está activada, haga un clic en un nombre del campo para que los valores de éste aparezcan en la lista de valores. Los nombres de campo se encierran siempre en corchetes cuadrados ([ ]). Si el valor que usted quiere emplear en la consulta no se encuentra en la lista de valores, escribalo en la caja de texto de la consulta.Por ejemplo, para seleccionar todas las casas de más de JO millones de habitantes, la consulta seria:Las cadenas, por ejemplo los nombres, se escriben siempre entre comillas en las consultas. Las cadenas no son sensibles a la caja, de manera que se puede seleccionar Brasil asl:Utilice • como comodín de caracteres múltiples. Por ejemplo, para seleccionar 'Nicaragua' podría hacerse la consulta: ([nombre_pais] = \"nica *\")Utilice? en una cadena como comodín de carácter único. Por ejemplo, para encontrar Catherine Smith y Katherine Smith, utilice:([nombre]= \"?atherine smith\")Para seleccionar todas las ciudades cuyos nombres comiencen con letras de la M a la Z, podría usar la consulta:Los campos de fecha se tratan como objetos de fecha. Para seleccionar todas las fechas anteriores al día de hoy, puede usar la consulta:Para seleccionar todas las fechas anteriores a una fecha específica, usaría la consulta:([fecha] < Date.Make(\"03/15/ 1971 \", \" MM/dd/yyyy\")) Para obtener más información, consulte la sección correspondiente a ejecución de operaciones en los campos de fecha.Se pueden formular consultas complejas combinando expresiones con los operadores \" and\" y \"or\". Por ejemplo, para seleccionar todas las casas que tienen más de 500 metros cuadrados y un garaje, haga la siguiente consulta:([area) > 500) and ([garaje]= \"S\") Utilice el operador \" not\" para excluir. Por ejemplo, para seleccionar todos los departamentos de Colombia excepto Narifio, haga esta consulta:([nombre_pais] =\"Colombia\") and (not ([nombre_dept] =\" Nariño \"))Una consulta puede comparar los valores de dos campos. Por ejemplo, para encontrar todos los condados cuya población esté descendiendo, baga esta consulta:Es posible incluir cálculos en las consultas. Por ejemplo, para encontrar los condados cuya densidad de población sea menor que 25 personas por kilometro cuadrado o igual a ese valor, se haría la consulta siguiente:([pob 1990]/area <=25)Generalmente ArcView evalúa una consulta de izquierda a derecha, pero las expresiones encerradas entre paréntesis se evalúan primero. Por ejemplo, la consulta:se evaluará de manera diferente a la siguiente:Opciones de la caja de diálogo Fields (campos). Esta opción presenta una lista de campos en el tema o cuadro que usted esté consultando. Si está activada la opción Update Values (actualizar valores), haga clic una vez en un campo para que todos sus valores aparezcan en la lista de Values (valores). Haga doble clic en un campo para colocarlo en la caja de texto de la consulta. Si usted escribe el nombre del campo en vez de hacer clic en esta lista, los nombres de campo no son sensibles a la caja, de manera que se permite escribir \"Area\", \"area\" o \"AREA\".Los campos que han sido ocultados no aparecen en la lista de Fields (campos). Si se han definido equivalentes de los nombres de campo, éstos aparecen en la lista de Fields (campos).Operators (operadores Los operadores matemáticos\"+,-,*, f' y algunas expresiones arbitrarias de \"Avenue\" también pueden usarse en las consultas.Para encerrar una expresión entre paréntesis, selecciónela en la caja de texto de la consulta haciendo un clic sostenido sobre ella y arrastrándola con el ratón; luego haga un clic en el operador ( ). La parte seleccionada de la consulta quedará encerrada entre paréntesis.Values (valores). Esta función presenta una lista de valores del campo escogido. Solamente aparecen los valores exclusivos. Por tanto, si cinco características o registros del tema o cuadro que usted está consultando tienen el mismo valor para el campo elegido, este valor sólo aparece una vez en la lista de valores. Haga doble clic en un valor para ubicarlo en la caja de texto de la consulta. Si el valor que usted quiere emplear no está en la lista, escrlbalo en la caja de texto de la consulta. No se pueden visualizar valores de cuadros que contengan más de 30,000 registros.Update Values (actualizar valores). Por defecto, la lista de valores se actualiza cada vez que usted elige un campo. Si hay muchos valores, su actualización puede requerir un poco más de tiempo. Desactive esta opción con un clic si no desea actualizar los valores. Esta función es muy útil cuando la consulta compara un campo con otro y no es necesario ver los valores, o cuando se desea escribir valores especlficos en la caja de texto de la consulta en vez de escoger valores en la lista de Values.Query Text Box (caja de texto de la consulta). En esta caja aparece la consulta a medida que ésta se elabora.Si usted emplea Query Builder (constructor de consultas) para seleccionar ya sea características en una vista o registros en un cuadro, dispone de las siguientes opciones para formular su consulta:New Set (conjunto nuevo). Hace un nuevo conjunto de selección que contiene las características o los registros elegidos en su consulta. Las características o los registros que no estén en este conjunto serán eliminados.Add to Set (adicionar al conjunto). Af'iade las características o los registros que se hayan seleccionado en su consulta al conjunto de selección ya existente. Si no existe un conjunto de selección, las características o los registros especificados en la consulta se convierten en un nuevo conjunto. Elija esta opción para ampliar la selección.Select from Set (seleccionar del conjunto). Selecciona las características o los registros de su consulta a partir del conj unto de selección existente. Solamente las características o los registros de este conjunto existente que se seleccionen en su consulta permanecerán en el conjunto de selección. Elija esta opción para reducir su selección.Consultas para definir una selección de características para un temaSi usted emplea Query Builder (constructor de consultas) en la caja de diálogo de Theme Propertíes (propiedades de l tema) para definir una selección de características para un tema, verá los iconos OK y Cancel en vez de New Set (conj unto nuevo), Add to Set (adicionar al conj unto) y Select from Set (seleccionar del conjunto).OK (aceptable). Selecciona las características de su consulta y hace que esta consulta sea la defmición de la selección de características para el tema. Solamente las características que cumplan con esta definición serán representadas en el tema. La consulta se aí'lade a la caja de diálogo de Theme Properties (propiedades del tema).Cancel (cancelar). Cierra Query Builder (constructor de consultas), sin ejecutar la consulta.Usted puede copiar y pegar las consultas. Por ejemplo, usted puede emplear Query Bullder (constructor de consultas) en una vista para hacer una consulta sobre un tema, y luego decidir usar esta consulta como la definición de la selección de características del tema. En este caso, puede copiar la consulta, abrir Query Builder (constructor de consultas) en la caja de diálogo de Theme Properties y pegar la consulta. Para copiar toda la consulta o parte de ella, seleccione lo que usted quiere copiar y luego pulse CTRL+C. Para pegar, pulse CTRL+V. Su plataforma puede tener otras formas de acceso directo a estas operaciones por medio del teclado, por ejemplo, en Windows se puede también copiar con CTRL+INS y pegar con SHIFT+INS.Cuando está haciendo una consulta sobre una vista o un cuadro, puede usar todavía los demás componentes de su proyecto mientras Query Bullder (constructor de consultas) esté abierto. De este modo usted puede encontrar los valores, datos y estadísticas que quiera usar en la consulta. Por ejemplo, si usted quiere seleccionar en una vista todas las regiones de ventas ~ue tengan un ingreso superior al promedio, puede emplear Statlstics (estadísticas) en el campo Revenue (ingresos) en el cuadro de atributos de tema para encontrar el ingreso promedio; luego regresará al Query Builder (constructor de consultas) para introducirlo en su consulta. (Cuando esté usando Query Builder (constructor de consultas) de la caja de diálogo de Theme Properties (propiedades del tema) usted no puede hacer esta operación; debe cerrar Query Builder (constructor de consultas) y la caja de diálogo de Theme Propertles (propiedades del tema) antes de poder usar otros componentes del proyecto).Después de emplear Query Builder (constructor de consultas) para seleccionar características en una vista, utilice Zoom to Selected Features ~(ampliar las características seleccionadas) para visualizar mejor el alcance de las características que usted ha seleccionado.Recuerde que los datos incluidos en el Atlas CD son en su mayoría visuales. Usted necesita entonces tener una pantalla y plaqueta gráfica que le permitan obtener una buena resolución, en caso contrario la visualización será de mala calidad.Recuerde cuando defina el \" tipo de letra\" bajo Windows de chequear si esta es \" letra pequeña\" o \" letra grande\". Si emplea letra pequeña conservara la estructura y diagramación de los recuadros y figuras. Si emplea letra grande Atlas CD puede alterar la diagramación y estructura de los recuadros y figuras.Recuerde que dada la gran cantidad de mapas e imágenes, el Data Publisher puede ser lento al abrir algunas de las aplicaciones. La única solución para mejorar el rendimiento, es cargar todo el Atlas CD en su disco duro. Para esto se necesita de una capacidad de 500 Megabytes (MB) en su disco. SI no tiene la capacidad en su disco duro, por favor tenga paciencia al emplear algunas de las aplicaciones.In recent years a world consensos has been reached on the need for sustainable development. Such a changed perspective on development implies new demands, one ofwhich is the production of information that will play a critica! role in providing a fmn basis for decision making and monitoring development. To respond to this demand, CJAT and UNEP made a collaborative agreement in 1995 to prepare indicators for monitoring development and the environment in Latín America and the Caribbean. These indicators would serve as tools to support decision making and planning. The project Environmental and Sustainability Indicators: Outlook for Latín America and the Caribbean resulted from this collaborative agreement.The project aims to develop products, like this CD-ROM, with which:• Data, statistics, and indicators can be related to management and administrative needs.Sets of data and indicators can be integrated on a geographical basis to support decision making at different \\evels (country, ecoregion) and scales (local, national, regional, worldwide). • lnformation exchange and quality of the information used in decision making and planning can be improved and made easier. • Useful regional, national, and local information can be disseminated for decision making.The production ofthis CD-ROM has been made possible only with the help and cooperation ofvarious individuals and institutions. Without the financia! and institutional support, data and information, as well as the comments, criticism and advice , it would have been impossible to have completed this work.Obviously the fmal responsibility rests with the authors.A special mention to all ofthe institutions that have provided the funds that allowed us to develop this CD-ROM: lntemational Center for Tropical Agriculture (CIA T) and United Nations Environment Programme (UNEP). We are also grateful to the following institutions for providing data and information: Land Management Unit (CIA T), GIS Unit (CIA T) To install the software run setup. exe from the root directory of the CD-ROM. This will initiate the setup process and will ask where you wish to install Atlas CD. You will also be given the choice of installing the data (DATA) on your machine or leaving it on the CD-ROM.Note: Atlas CD is designed so that all the data can be accessed from the CD. However you may wish to move the data toa more convenient location especially ifthere are users who do not have access toa CD reader. lf you choose to install the data base on the computer you will need to have 500Mb free on the hard disk.Run the file X: \\data \\modelo \\install \\setup. exeThis initialises the setup program for the Model.You will be asked to choose a directory into which the program will be installed. Choose the default directory.The setup routine will then copy Landuse. exe to the specified directory and will also copy a number of files to your WINDOWS\\SYSTEMdirectory. These files enable the Model to be run.This process has to repeated on each machine which will run the indicators package.There are a number ofways to start the ArcView project:When you install Atlas CD a program group and item will be created for your windows desktop. Doubleclick on the Atlas CD icon to start the application.Alternatively from File Manager double-click the unepcia t. ex e icon in the X: \\a tlascd\\bin directory.Note: Atlas CD may not function correctly ifyou have ArcView 3.0 already installed. Ifyou discover any problems please visit the project home page or contact us (see page 40)Atlas de mdrCaOores ambrentales y de sustentabrhdad On opening the project you wiU be asked to provide the drive on which the data are stored. All of the data must be on the same drive and the original directory structure must be retained, otherwise the path names will be incorrect and the maps will not load properly.¿En cuál unidad de disco almacena los datos ? (On whlch drive are the data stored ?1:Atlas de indiCaDores ambientales y de sustentabrhdad Y ou will also be asked to en ter the size of the monitor screen on which you are viewing the data. This is in order to display the correct scale of mapping on the screen. You are given five options, ranging from laptop size toa large screen. lfyou are unsure, or non e of the options are appropriate, then click 'Other'. You will then be prompted to enter the width and height ofthe screen respectively7Elige la dimensión de la pantalla (Choose your screen size) :Mediana The project now loads and you will see the 'unepciat.apr' project window . The window will show the views that are in the project.There is only one view. This is named 'Atlas CD'. This is opened automatically when you open the project.When you open the view you will notice that the menus change and more buttons appear on the toolbar.The frrst six menus are conventional ArcView menus.The next seven menus relate onJy to the 'Atlas de indiCaDores ambientales y de sustentabilidad para América Latina y el Caribe' project.These menus are: Click on Guía de Uso A pull down menu appears. There are two options from the pull down menu: Indicadores Programa.Click the Programa option to open the guide you are now reading.There are three sections to the guide:This section contains a number oftopics relating to the background ofthe project, ranging from the state of the environment and region to the conceptual model for the project.This section contains analysis, tables and figures for all ofthe indicator variables in the pull-down menus. Each section highlights the most important indicators and should act as a useful guide for policy-makers. The section also contains technical notes explaining how the various maps have been createdThe glossary provides definitions for many ofthe terms that are used in the text or which describe indicators.Each section contains many hypertext Iinks. Those links in green take you to another help topic. Those in blue open relevant figures and links in gold open tables. There are also links highlighted in red, just click on the hypertext anda map is loaded in the Atlas CD -..iew window.You can retum to Atlas CD at any time. Just close the guide or leave it open and click on the Atlas CD window.Click Datos Basicos at the top of the screen. A pul! down menu appears. There are 11 options from the pull down menu: To display any of these themes click the name in the list. The map ofthe theme appears on the right hand side ofthe view, whilst the legend appears on the left in the One of the most useful functions that the package will perfonn is the overlaying of various maps. This is a very cumbersome process by hand but is incredibly simple using GIS. Users simply load one theme after another, then decide which appears on top ofthe others. Overlaying maps allows users to perfonn simple visual analyses. The analysis can be improved by zooming-in to the area of interest.lfyou wish to see more explicit infonnation about a particular map feature (point, line or area) you should flrst actívate the map theme by clicking the name in the table of contents. Then click the lnfo tooJI.O J on the too) bar and click the feature on the map. This will bring up the entry for that feature in the associated attribute table (see ArcView on-line help topic \" ldentifying features on a view\" for further details).There are severa) ways to select features on a view. You can select them according to their location, according to their attribute values, or by selecting their records from atable. You can select features from one or more themes. Before you can select features from a theme, you have to click on the theme in the Table of Contents to make it active.Features turn yellow on the view when they are selected and remain highlighted until a different selection is made, or until they are deselected.Use the Select Feature toollfml to select features on the map.Find is a quick way to select a particular feature of interest. For example, ifyou are working with an indicator ofthe large cities and you want to select 'Buenos Aires', simply click the Find button icon IAI and type that name into the dialogue box. The program wi ll search the attributes of all the cities to see if the name appears. The first feature to have that name will be selected and the display will centre on the location of the feature. lf th is is not the correct featu re repeat the sequence and the program will flnd the next feature that has that name.Use a query wben you want to select features according to their attribute values. For example, you can build a query (see page 35) to select all the states in the Brazil with populations greater than 1 O million.Most indicators in the package ha ve a table of attributes connected to the map. The val u e of the attributes defines the colour and texture ofthe map. You can select features on the map by opening the attribute table and selecting the records. When they are selected the records in the tab le tum yellow and the features on the map also tum yellow. To open the tablif must flfSt ensure that the indicator is active in the Table of Contents. Then click the table button icon . You will now see the attribute table for the indicator. When you click and selecta record from the table it is h.ighlighted, ifyou retum to the View ofthe map and click the 'Zoom to selected features' ~ button icon the map will zoom to the extent of any fea tu res that you have just selected.(see ArcView on-line help topic \"Selecting features on a view\" for further details).Atlas CD allows users to perform more advanced queries of their data according to how different indicators interact.The example here will show how you can select all ofthose centres ofbean production that lie within 5 km of specific roads in the road network. Zoom in to the selected features using the ~ button on the toolbar.Nearly all ofthe indicators have source information. This information includes the name ofthe source (Fuente), the date of the so urce {Fecha), and the type of data (Tipo de Datos).To view the source information you should first actívate the map theme by clicking the name in the table of contents.Then click the Examine button ~ on the tool bar .This brings up an information dialogue box containing_ the source, date and type of data for the indicator.Click 'OK' to move to the next theme (ifyou have more than one theme active) or fmish .To cboose a life zone just click the radial button to the left of the life zone code.When you choose the life zone the model is run. Y ou can then view the graphs of projected landuse, read the analysis of the projected changes, retum to change the scenario or close the program.To view the graphs click the Grafica button.This opens up a new window. lnitially there is no graph showing. To see a graph you must first choose an indicator from the Presión (pressure), Estado (state), Impacto/Efecto (impact/effect), or Respuesta (response) boxes.In the Presión box there are:• Cambios en Uso de Tierra (Ch ange in Land Use) C lick the button to the left ofany ofthe land use types that you wish to see displayed. Then click the Dibuja button. The projected land use changes w ill appear in the graph.In the Estado box there are:• Superficie en Uso (Surface in Use)• Superficie de Bosques (Forest surface)For the Superficie en Uso indicator, as with Cambios en Uso de Tierra, you will have to choose which land use types are displayed in the graph. The graphs provide a very good visual analysis of the models' proj ections. To open the Analisis window click the button from either the graph window or the life zone window.The Analisis window is split into a set of tables and text. The tables summarise the indicators shown in the graphs. There is also an interpretation for bolh scenarios, highlighting the most important results.Date fields are treated as date objects. To select all dates before today, you could use:To select all the dates before a particular date, you could use:([date] < Date.Make(\"03/15/1971\", \"MM/dd/yyyy\"))See Perfonning operations on date fields for more infonnation.Complex queries can be built by combining expressions together with the And and Or operators. For example, to select all the houses that ha ve more than 1 ,500 square feet and a garage, use the query:( [area] > 1500) and ([garage]= \"Y\")Use the Not operator to exclude. For example, to select al! the New England states except Maine, use the query:( [sub_region] =\"N Eng\") and (not ([state_name] = \"Maine\"))Queries can compare the values oftwo fields. For example to fmd all the counties with a declining population, use the query:Calculations can be included in queries. For example, to find the counties with a population density of less or equal to 25 people per square mile, you could use the query:( [popl990]/area <= 25)Nonnally ArcView evaluates a query from left to right, but expressions that you enclose in parentheses are evaluated first. For example, the query:will be evaluated differently from: Performing a query on a view or a tableIfyou are using the Query Bullder to select features on a view or records in atable, the following options are available for perfonning your query:New Set Makes a new selected set containing the features or records selected in your query. Features or records not in this set are deselected.Add To Set Adds the features or records selected in your query to the existing selected set. Ifthere is no existing selected set, the features or records specified in the query become a new set. Use this option to widen your selection.Select From Set Selects the features or records in your query from the existing selected set. Only those features or records in this existing set that are selected in your query wiJI remain in the selected set. Use.this option to narrow down your selection.Performing a query to define a feature selection for a themeIfyou are using the Query Builder from the Theme Properties dialog box to define a feature selection for a theme you will see OK and Cancel buttons instead of New Set, Add To Set and Select From Set.OK Selects the features in your query and makes this query the feature selection defmition for the theme.Only those features that meet this defmition will be represented in the theme. The query is added to the Theme Properties dialog box.Cancel Closes the Query Builder without running the query.You can copy and paste queries. For example, you migbt use the Query Builder on a v iew to query a theme then decide to use this query as the theme's feature selection defmition. La conferencia de las Naciones Unidas sobre Medio Ambiente y Desarrollo (UNCED-92) condujo a un consenso general acerca de la necesidad de un desarrollo sostenible. Además de un cambio de perspectiva acerca del proceso de desarrollo, el concepto del desarrollo sostenible implica nuevas demandas. Una de estas es la producción de información, la cual debe jugar un rol critico, puesto que ella puede proveer de bases firmes al proceso de toma de decisiones y al seguimiento del proceso de desarrollo. Es asf como el capitulo 40 (Información para la Toma de Decisiones) de la Agenda 21 hace un llamado para el desarrollo de indicadores para el desarrollo sostenible. En particular se pide en el nivel nacional, que los paises, y en el niv el internacional, que las agencias internacionales y los organismos no gubernamentales, desarrollen el concepto de indicadores de desarrollo sostenible e identifiquen los indicadores aptos para monitorear el proceso de.desarrollo.Para el proceso de la toma de decisiones y el análisis y seguimiento de las políticas y estrategias de desarrollo existen una serie de datos, estadísticas e indicadores económicos y sociales que son usualmente utilizados en el nivel regional y nacional (UNEP, 1993;UNDP, 1996;World Bank, 1996;WRI, 1996). Sin embargo información ambiental equiv alente no existe o no se encuentra disponible para los usuarios, lo que impide que la toma de decisiones se efectúe tomando en cuenta todos los componentes y características del proceso de desarrollo. No obstante , en los niveles regional y nacional existen en muchos casos suficientes datos y estadísticas ambientales y socioeconómicas que permitirían incrementar el uso de la información dentro del proceso de toma de decisiones. Sin embargo estos datos y estadísticas están muchas v eces subutilizados o comprometidos por la ausencia de marcos metodológicos comunes, aceptados y validados int ernación al, r eg i o n al y naciona lmente.Para mejorar esta situación se requiere la elaboración de información que permita el seguimiento del desarrollo. Esta información debe ser concebida como un conjunto de datos e indicadores ambientales y socioeconómicos relacionados que permitan el monitoreo del proceso de desarrollo a múltiples escalas geográficas. De esta manera se podrá apoyar, mejorar y hacer más eficaz &1 proceso de l a toma de decisiones y la planificación así como incrementar el intercambio, la difusión y la comunicación de la información al nivel de los usuarios. Además se podrá guiar y perfeccionar el proceso de recolección y toma de datos, así como ayudar a identificar áreas y temas en donde la información disponible es inadecuada o inexistente. De esta manera la información producida debe permitir:1) Determinar los cambios y condición del medio ambiente y los recursos naturales en relación con el proceso de desarrollo y la sociedad .2) Dar un diagnóstico sobre las causas y efectos potenciales de los problemas actuales detectados o los cambios en el estado del medio ambiente y la sociedad a fin de elaborar las respuestas y acciones adaptadas .3) Pronosticar y predecir los impactos actuales y futuros de las actividades humanas sobre el medio ambiente para definir las estrategias y políticas alternativas.Par a hacer operativas estas funciones se ha elaborado el presente Disco Compacto que constituye un sistema de infor mación sobre la base a un conjunto de datos básicos e indicadores que permi ten:1) Conectar los datos, estadísticas e información relacionada con las necesidades de manejo y gestión en los niv eles local, nacional y regional.2) Integrar conjuntos toma de decisiones en ecoregión) y escalas 3) Identificar vacíos tareas de recolección de datos en una base geográfica para apoyar el proceso de función de los diferentes niveles (país, ecosistema, (local, nacional, regional, global). o duplicación en la información y hacer más eficaz las de datos en los niveles nacional, regional y global.4) Mejorar y facilitar el intercambio y la calidad de la información utilizada en el proceso de la toma de decisiones y la planificación. S) Comunicar a los diferentes tipos de usuarios información regional, nacional y local útil para la toma de decisiones.El contenido y productos del sistema incluyen varios tipos de información, que va desde un marco conceptual de referencia y la definición de los problemas y áreas prioritarias hasta conjuntos de datos e indicadores georeferenciados, estadísticas y análisis. Además como no todas las instituciones y paises se encuentran en el mismo estado de desarrollo para la adquisición, elaboración y uso de datos e indicadores el sistema puede servir como punto de referencia y de apoyo e intercambio de información y metodologías. Al mismo tiempo el sistema provee de otras herramientas como proyecciones, modelos y escenarios útiles para la definición de objetivos y metas de desarrollo y calidad ambiental.No obstante, cabe anotar que la difusión y uso del conjunto de indicadores para la toma de decisiones está sujeto al desarrollo de un proceso de intercambio entre productores y usuarios de estas herramientas. Esto implica un diálogo práctico y una comunicación continua entre los diferentes actores, para establecer nuevas necesidades y mantener la discusión y análisis para cada etapa del proceso de toma de decisiones para las cuales los indicadores fueron elaborados. Este proceso requiere, además de un intercambio fluido de informa~ión y de un proceso abierto de armonización, de un delicado balance acerca de la validez científica, la aceptabilidad política y la factibilidad económica y técnica para el desarrollo y uso de estas herramientas. Con este fin se ha creado una página en Internet para recibir y conocer sus necesidades, reacciones, comentarios, sugerencias e información, en la siguiente dirección: http://www.ciat.cgiar.org/land/indicators/project.html De esta manera, el producto elaborado y la ventana abierta en Internet, nos dan la seguridad de poder contribuir a que los países, instituciones y personas de América Latina y el Caribe puedan convertir la información en acción para que el transito necesario hacia modelos de desarrollo sostenibles en la región sea una realidad.","tokenCount":"8141"} \ No newline at end of file diff --git a/data/part_6/1333047526.json b/data/part_6/1333047526.json new file mode 100644 index 0000000000000000000000000000000000000000..b2f2f00733e16906133b9fd53ac5dbf1b15cfa41 --- /dev/null +++ b/data/part_6/1333047526.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"a74042deb8b8cfa87fda8b2de2ee9af3","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/4ba52f09-c986-4641-b836-8d073a73d24e/retrieve","id":"1603273682"},"keywords":[],"sieverID":"68ff2954-d285-4199-bff2-f9f0c7a8460f","pagecount":"4","content":"In India, the CGIAR Initiative on Nature-Positive Solutions works in remote parts of Western India's Maharashtra State. NATURE+ and its partners selected several sites (called clusters) for their high potential for implementing nature-positive activities to improve nutrition, climate resilience, food security and to conserve nature. People in these tribal areas also have rich traditional knowledge that is key to natural solutions.The CGIAR Initiative on Nature-Positive Solutions aims to re-imagine, collaboratively create and implement nature-positive solutions for agrifood systems that equitably support food and livelihoods and ensure that agriculture is a netpositive contributor to biodiversity and nature.There are several challenges to a nature-positive transition in India. There is limited local-level data on available resources and their utilization for naturepositive land use. There is a general lack of awareness on the efficient use of resources. There is little to no market for sustainable circular economy business models. Financing for nature-based solutions at the individual or farm level remains low.The Initiative works in collaboration with highly capable partner organizations that specialize in activities related to agricultural management and production. In addition, NATURE+ collaborates with relevant on-the-ground stakeholders to assess, develop, implement, and sustain nature-positive solutions in agri-food systems to ensure income generation, improve biodiversity, and deliver a net-positive impact of agriculture on nature. The Initiative creates opportunities to access the broader food basket through more nutrient-rich crop varieties to help develop more resilient and adaptable farming and seed systems.To provide strong institutional support in India for a transition to nature-based solutions, NATURE+ works to enhance income opportunities (particularly for women, youth and indigenous peoples) through value chains related to seeds, crops, fruit trees, waste, payment for ecosystem services, and public procurement. Improved restoration and soil management efforts, as well as improved recycling value chains, will create highly valued carbon sinks and reduce greenhouse gas emissions.In India, the CGIAR Initiative on Nature-Positive Solutions works in two clusters, Akole and Nandurbar, Maharashtra.Outlook 2024 • Collaborative design and implementation of pilot business models based on surveys and data collection, technology identification, and awarenessgeneration workshops for circular bioeconomy activity.NATURE+ completed a community-led planting drive on communal land, which included local species selected by the community. Some 35 hectares and 4,000 saplings were planted. NATURE+ built a priority species list of 237 tree species ideal for restoration and full trait and suitability modeling was completed for 177.The 237 priority tree species will be fully added to the Diversity for Restoration tool, and forest restoration activities will continue in the clusters. NATURE+ and partners will complete a comparative study on biodiversity and ecosystem services under grazed andA circular bio-economy innovation hub was launched with an accompanying website and the first meeting of the hub was conducted with new stakeholders in India.Agricultural and livestock waste was identified in microwatersheds to explore potential for circular bioeconomy activities. The Initiative conducted capacity building on agri-livestock waste management in local languages and farmers; 80 farmers were trained on management strategies.The Initiative will continue the development of the circular bioeconomy innovation hub and develop a technical brief on the hub, along with a training manual for biogas. NATURE+ will continue the identification of potential stakeholders for the hub. In the selected sites, researchers will identify the gaps in the existing bioeconomy interventions and will help address these gaps through appropriate business models.NATURE+ collected cross-cutting data for all work packages and conducted 1,227 quantitative household surveys related to crop area, livestock, income source, food, energy, fuel and waste management, along with 27 community surveys for land use and other services. Furthermore, qualitative data collection were collected using 20 key informant interviews and 20 focus group discussions with men and women farmers and results were reported to the NATURE+ Pause and Reflect meeting at the end of the year. Natural resource mapping for nature-positive solutions was completed.The Initiative will complete a preliminary analysis of the survey data and a technical report on the quantitative results will be prepared. Qualitative results will be reported in a discussion paper and journal article focused on gender and nature-positive solutions. A journal article has been prepared based on mapping above-ground carbon storage and sequestration using the Integrated Valuation of Ecosystem Services and Tradeoffs model in a separate watershed that will have implications for the NATURE+ sites.NATURE+ model farms will be created using the integrated farming system approach at selected sites. The Initiative will develop a composite index to study nature-positive indicators for land, soil, water, and agrobiodiversity. Support for an existing seed bank in Akole cluster will continue, and a new seed bank will be developed. The Initiative will support the establishment of an on-farm conservation center for Rabi (or \"winter\" crops, which include wheat). Research trials will assess drought-tolerant microorganisms, biochar, and hydrogel impact on the growth and yield of gram (chickpea) under drought stress on research plots. The Initiative will do measurements of hydro-meteorological parameters and installation of equipment at clusters for climatesmart watershed strategies. Furthermore, 50,000 cactus cladodes (cactus shoots) will be planted. How NATURE+ works in India non-grazed forests. Researchers will assess restoration impacts on ecosystem services and livelihoods. Additionally, the impact of a biogas unit -which supplants tree-based energy -on forest conservation will be assessed and reported. A national restoration workshop will discuss current restoration initiatives and gaps with scientists, practitioners, and stakeholders.","tokenCount":"878"} \ No newline at end of file diff --git a/data/part_6/1368342057.json b/data/part_6/1368342057.json new file mode 100644 index 0000000000000000000000000000000000000000..5c17da756d0dfbf1464eb19ba00d42daf13c35f1 --- /dev/null +++ b/data/part_6/1368342057.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"7406d311a2ea25e04e8dda9e2882e55a","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/a33c11ba-8a75-46e5-8f3b-55a1519d59ee/retrieve","id":"-1108745195"},"keywords":[],"sieverID":"674e7a88-674b-4b2f-a7f3-746265f0e70f","pagecount":"12","content":"Propuesta de un año de referencia para los compromisos voluntarios, para la producción libre de deforestación en la Amazonía peruana POLÍTICAS EN SÍNTESIS No. 76 1 2 3 4La selección de un año de referencia, para la producción libre de deforestación, es fruto del consenso de los actores públicos y privados, en el marco de los compromisos voluntarios, sobre una producción libre de deforestación. Con ello, se busca el beneficio de los agricultores y ganaderos amazónicos, los bosques y la competitividad de la Amazonía peruana.El año de referencia propuesto ha considerado criterios técnicos, entre estos: i) la viabilidad del monitoreo para los compromisos de no deforestación, a escala de finca, en la Amazonía peruana; ii) factibilidad de implementar mecanismos robustos de compensación; iii) las salvaguardas para evitar que sean deslegitimados y iv) las nuevas exigencias de las propuestas de regulaciones internacionales, sobre la deforestación importada, en los mercados de demanda de las principales cadenas de valor amazónicas.En las consultas con actores claves, tanto de los sectores públicos y privado de las cadenas priorizadas, se identificó que el 2020 (31 de diciembre del 2020) es la opción más factible para tomar como año de referencia, con respecto a los compromisos voluntarios para la producción libre de deforestación de las cadenas de valor de cacao, palma aceitera, café y ganadería en la Amazonía peruana.Para el cumplimiento del año de referencia, se necesitan condiciones habilitantes, como un marco legal adecuado, zonificación y ordenamiento forestal y la formalización de la propiedad, entre otros. Además, es recomendable la implementación de incentivos financieros y no-financieros, como el acceso al financiamiento, la asistencia técnica y un mejor precio por los productos de alta calidad.Al considerar que no todos los sectores se encuentran en una misma situación, se requiere una gradualidad estratégica, de modo que se pueda avanzar rápidamente en los escenarios menos complejos y diseñar soluciones para enfrentar los de mayor complejidad.En los últimos años, la comunidad internacional ha estado negociando varias propuestas normativas, con el fin de minimizar el impacto del comercio internacional en los bosques y el cambio climático. La Unión Europea, los Estados Unidos, el Reino Unido y la República Popular de China, entre otros, están debatiendo regulaciones para evitar la importación de productos y materias primas asociados a la deforestación y analizar los requisitos de trazabilidad para los commodities (p. ej. la soya, la carne bovina, el cacao, el café y el aceite de palma aceitera, entre otros) que se comercialicen en sus territorios. Estas propuestas de regulaciones se dan en línea con los compromisos globales, que buscan mitigar el cambio climático, como el Acuerdo de París y la Declaración de Glasgow sobre bosques y uso de la tierra, los cuales ponen sus reflectores sobre la deforestación, especialmente en regiones tropicales.A nivel nacional, el Gobierno peruano ha asumido múltiples compromisos para reducir la deforestación. El país es miembro de la Convención Marco de lasContribuciones Nacionalmente Determinadas (CND), cambio climático, deforestación, cadenas de valor (café, cacao, palma aceitera y ganadería), Amazonía peruana.por una Producción Sostenible (CPS) y del Acuerdo Cacao, Bosque y Diversidad, proponen un año de referencia para los compromisos voluntarios de producción libre de deforestación, producto de un proceso transparente y participativo, acotados en una primera etapa al cacao, la palma aceitera, el café y la ganadería, que promueve e identifica las condiciones apropiadas para implementarlos en la Amazonía peruana.La presente propuesta está basada en una revisión extensa de fuentes bibliográficas y entrevistas estructuradas, como parte de un estudio sobre el año de referencia para los compromisos sobre la producción libre de deforestación en la Amazonía peruana 3 . Posteriormente, la información ha sido validada a través de dos talleres de consulta con actores clave, donde se incluyen los tomadores de decisión del sector público, del sector privado y de los gremios representativos de las cadenas de cacao, la palma aceitera y el café; así como los actores de la sociedad civil 4 . Asimismo, esta propuesta es aplicable también a la ganadería u otras cadenas agropecuarias amazónicas.El Perú es uno de los diez países del mundo con mayor superficie de bosques, el segundo con mayor extensión de bosques amazónicos y el cuarto en bosques tropicales del mundo (FAO, 2020). Estos bosques se concentran en siete departamentos (Amazonas, Cusco, Huánuco, Loreto, Madre de Dios, San Martín y Ucayali), los cuales abarcan más del 92% del total nacional (MINAM, 2020). Además de ser una importante reserva de carbono, los bosques peruanos albergan una gran diversidad biológica y proveen bienes y servicios fundamentales, para el desarrollo del país y el bienestar de sus habitantes, en particular, de los pueblos indígenas u originarios.Naciones Unidas sobre el Cambio Climático (CMNUCC) y ha endosado el Acuerdo de París, a través del cual, se ha comprometido a reducir sus emisiones de GEI en un 40% para el año 2030, respecto al 2015. Para ello, se han definido medidas para alcanzar la Contribuciones Nacionalmente Determinadas -CND, con la finalidad de alcanzar esta meta y luego de aprobarse la Ley Marco sobre Cambio Climático -Ley n.º 30754, la Estrategia Nacional ante el Cambio Climático (ENCC), la Estrategia Nacional sobre Bosques y Cambio Climático (ENBCC) y la Estrategia Nacional sobre Diversidad Biológica (ENDB), las cuales proponen líneas y acciones estratégicas para conservar los bosques, y reducir las emisiones de gases de efecto invernadero (GEI), en el aprovechamiento del territorio y los sectores del cambio del uso de la tierra y la silvicultura (UTCUTS). Además, el país ha firmado la Declaración Conjunta de Intención (DCI) es un acuerdo voluntario de cooperación firmado por los Gobiernos de Perú, Noruega y Alemania para lograr la reducción de emisiones de gases de efecto invernadero producidas por la deforestación y degradación de los bosques en el Perú (ver Cuadro 1: Marco legal peruano vinculado a bosques, propiedad rural y cambio climático). A nivel regional, los gobiernos regionales amazónicos han suscrito la Declaración de Río Branco 1 , que busca reducir la deforestación en cada jurisdicción en un 80%, y han aprobado las Estrategias Nacionales de Desarrollo Rural Bajo en Emisiones (ERDRBE) 2 y los planes de competitividad libre de deforestación y bajo de emisiones GEI, para las cadenas de valor de palma aceitera, de cacao y chocolate. Lo anterior define una ruta estratégica para reducir la deforestación, incrementar la competitividad y sostenibilidad de la producción regional, y mejorar el bienestar de los ciudadanos amazónicos, al considerar la inclusión de las mujeres, los pueblos indígenas y los jóvenes.En este contexto, la Alianza de Bioversity International y el Centro Internacional de Agricultura Tropical (CIAT), en el marco del proyecto Sustainable Amazon Businesses (SAB) para abordar las causas de la deforestación financiado por IKI e implementado en coordinación con MINAM y MIDAGRI, la Coalición De acuerdo con la ENBCC, no es posible atribuir la pérdida de bosques húmedos tropicales de la Amazonía peruana a una única causa, directa o indirecta, u observar un único cambio de uso de la tierra; no obstante, es posible identificar las causas con mayor influencia en los mecanismos causales de deforestación y cambios de uso. La pérdida observada es el resultado de los mecanismos causales de deforestación, los cuales varían local y departamentalmente, y son dinámicos a lo largo del tiempo. En cuanto a las causas, las más frecuentes son las siguientes: la expansión agropecuaria, la apertura de vías y caminos, el narcotráfico, el acceso limitado al crédito, el aumento de la migración, la baja asociatividad, la implementación de políticas inadecuadas de titulación y de saneamiento físico legal de la propiedad agraria, el avance escaso en el ordenamiento forestal, la articulación intersectorial limitada y entre los niveles de gobierno, y las condiciones agroclimáticas adecuadas que incentiven el desarrollo de cultivos agropecuarios. Es prioritario implementar medidas para una producción sostenible con los pequeños agricultores, que poseen cultivos instalados en superficies menores a 5 hectáreas y cuya producción está destinada al mercado local o internacional; asimismo, con los medianos productores, quienes poseen extensiones de cultivos en áreas entre 5 y 20 hectáreas. Dado que la mayor pérdida de cobertura de bosques húmedos tropicales ocurre en áreas menores a 5 hectáreas (cerca del 70%), mientras que el 91% de la pérdida absoluta de cobertura ocurre en tierras sin categoría legal asignada, tierras de comunidades nativas, bosques de producción permanente, predios rurales y concesiones forestales maderables (MINAM, 2020).Los compromisos voluntarios son acciones conjuntas e individuales, las cuales contribuyen a la conservación de los bosques, y a evitar que la expansión de las áreas de producción agropecuaria sea un factor de deforestación. Estos tienen un enfoque de acción colectiva; es decir, firmados por empresas, organizaciones de productores, gobierno y sociedad civil, quienes se comprometen a eliminar la huella de deforestación en sus cadenas de valor. En este sentido, es relevante identificar y promover los diferentes incentivos que, más allá de la responsabilidad, permitan avanzar hacia la transformación productiva esperada, e involucrar a los actores de múltiples niveles y a los sectores en estos compromisos. La mayoría de los entrevistados para esta propuesta manifestó la importancia de los incentivos, sobre todo los financieros, para poder garantizar el éxito de los compromisos voluntarios de producción libre de deforestación. Ello debido a que, más allá de la buena voluntad y conocimiento de la importancia de mitigar el cambio climático, son conscientes de los esfuerzos necesarios para adoptar un modelo de producción libre de deforestación.De igual modo, es necesario reconocer que, para conseguir una producción libre de deforestación, se requiere que diferentes actores privados realicen cambios en sus procesos de toma de decisiones y en su modelo de negocio, de un business as usual, a un modelo de negocio libre de deforestación y bajo de emisiones GEI. Para el desarrollo de un modelo de negocio orientado a la conservación de los bosques, es necesario lograr una visión común de los actores vinculados a la cadena de valor del producto, desde el productor hasta el consumidor final, y al pasar por los proveedores e inversionistas. De esta forma, las decisiones de estos actores contribuirían al objetivo común de frenar la deforestación y conservar los bosques. Esta visión común debe ser asistida con la información, incentivos y herramientas necesarias, para que la producción y el consumo de productos cultivados en la Amazonía no generen deforestación de los bosques. Otro aspecto fundamental, para desarrollar un modelo de negocio libre de deforestación, es contar con un sistema de monitoreo y trazabilidad del producto que, por una parte, permita verificar que los cultivos no originan deforestación. 5 (ver Cuadro 3: Resumen de las principales opciones de incentivos y propuestos por los entrevistados, para catalizar los compromisos de deforestación voluntarios y buscados por la Coalición por una Producción Sostenible-CPS).5 Ver para palma: https://hdl.handle.net/10568/109750 y para cacao: https://hdl.handle.net/10568/109751Elaboración propia con base en el informe \"Alianza Colombia TFA (2021) Cero deforestación en Colombia: ABC de las cadenas cero deforestación de palma, cacao, carne y leche en Colombia.Mecanismo de control legal sobre empresas y asociaciones agropecuarias.Una propuesta para una regulación nacional o regional.Un esquema de certificación.Un sello sostenible que certifica que la cadena es cero desforestación.Un año de referencia para compromisos voluntarios de acción colectiva.Manifestación voluntaria de empresas, asociaciones, autoridades gubemamentales (nacionales, regionales y locales) hacia la cero deforestación.Compromiso gradual para acelerar la transformación del sector agropecuario amazónico hacia la cero de deforestación.Iniciativa de referencia (lecciones aprendidas y casos de éxito) para escalar incentivos positivos.Para qué es Qué no es• 2014: año de referencia para la Roundtable on Sustainable Palm Oil (RSPO), la cual es el estándar global para palma aceitera sostenible. Este año también enfrenta el reto de contar con información espacial de alta resolución; además, no permite abarcar a una mayor cantidad de productores.• 2017: año de referencia por la creación de la Coalición por una Producción Sostenible.• 2018: año de referencia por la disponibilidad de imágenes satelitales de alta resolución e, incluso, de acceso gratuito.• 2020: año de referencia que se alinea a las exigencias del mercado (p. ej. Unión Europea); además, cuenta con mayor disponibilidad de información espacial de alta resolución, y permite a las empresas y productores vinculados a un modelo de negocio cero deforestación concentrarse en las acciones que aseguren no deforestación asociada a sus cultivos, pensando en el futuro, en vez de centrar esfuerzos en documentar deforestación histórica que es costosa y con grado de incertidumbre dada las pocas imágenes de alta resolución de años atrás.A continuación, se presentan los cinco escenarios de los años de referencia (ver a continuación Cuadro 4) propuestos por los actores consultados:• 2011: año de referencia consistente con el utilizado para el otorgamiento de los Contratos de Cesión en Uso para Sistemas Agroforestales (CCUSAF), en el marco de la Ley Forestal y de Fauna Silvestre, y la referencia para el reconocimiento de la producción agropecuaria sostenible familiar, como una actividad elegible como REDD+. Es importante precisar que el año de referencia, para el otorgamiento de los CCUSAF, tiene por objetivo definir una línea base para otorgar un título habilitante forestal; por lo tanto, no adecuado para áreas fuera de los CUSAF, como los predios privados con bosques remanentes. La ventaja de usar el año de referencia del 2011 es que es consistente con el marco legal REDD+ y el forestal peruano, pero excluye a una gran área con bosques remanentes, que pueden ser conservados, y a la gran mayoría de productores agropecuarios amazónicos. De igual modo, es importante señalar que seleccionar este año de referencia trae consigo la complejidad y altos costos para conseguir imágenes satelitales de alta resolución, a escala de una parcela agraria o finca. Bosques: son tierras que se extienden por más de 0,5 hectáreas; están dotadas de árboles de una altura superior a los 5 metros y de una cubierta de dosel superior al 10 por ciento, o de árboles capaces de alcanzar esta altura in situ. No incluye la tierra sometida a un uso predominantemente agrícola o urbano. Justificación: es la definición bajo la cual se mide la cobertura de bosques tropicales en el Perú y se reporta a la Convención Marco de Naciones Unidas sobre el Cambio Climático (CMNUCC), y la definición internacional que será utilizada por las regulaciones internacionales de deforestación importada. 2Bosques regenerados (segundo crecimiento): que se vieron sujetos a impactos significativos en el pasado (por ejemplo, por agricultura, crianza de ganado, plantaciones de árboles o tala intensa), pero donde las principales causas del impacto han cesado o se han reducido de forma significativa. En estos, el ecosistema ha logrado recuperar mucha de la composición, estructura y función ecológica del mismo, anteriormente, o de otros ecosistemas naturales contemporáneos. 3Deforestación: eliminación de la cobertura forestal de un bosque natural ocasionada por el ser humano o la naturaleza. Justificación: definición utilizada por la LFFS (Ley forestal y de Fauna Silvestre) que es equivalente a las definiciones utilizadas en las propuestas de regulaciones internacionales. Nota: aquí es relevante precisar que el término bosque natural excluye a las plantaciones, pero incluye a los bosques primarios, bosques secundarios y purmas; por tanto, es importante contar con una buena línea base y una zonificación, con fin de no considerar como una buena práctica agrícolas a la deforestación.Cadenas productivas libres de deforestación: se refiere a la responsabilidad continua, debido al riesgo y el impacto negativo causado por una serie de acciones del productor al consumidor, en la cadena de suministro. Los esfuerzos de sostenibilidad también están orientados a mejorar la imparcialidad y los beneficios positivos para los proveedores (productores), trabajadores, clientes, usuarios finales y cualquier otra parte interesada. La sostenibilidad ofrece a las empresas la oportunidad de forjar nuevas relaciones comerciales y desarrollar competencias básicas más sólidas. • Consistente con el año propuesto en los contratos de cesión en uso, para sistemas agroforestales en LFFS• Utilizado como referencia por algunos productores de palma aceitera en la Amazonía peruana• Consistencia con objetivos de la Coalición por una Producción Sostenible (CPS)• Mayor disponibilidad de información satelital que facilita el monitoreo de los compromisos de producción, libre de deforestación, a un menor costo y mayor velocidad• Consistente con propuesta de la Organización Internacional del Cacao (ICCO, por sus siglas en inglés)• Mayor disponibilidad de información satelital que facilita el monitoreo de los compromisos de producción, libre de deforestación, a un menor costo y mayor velocidad• Aceptado por la comunidad internacional para fines comerciales• Posibilidad de incluir mayor cantidad de productores y productoras y, por lo tanto, conseguir mayores compromisos para la conservación de bosques• Por la antigüedad de los cultivos (palma aceitera, 3 años; cacao, 3-4 años y café, 3-4 años) incluye a todas las áreas actuales bajo producción• Excluye a productores que no puedan demostrar que sus parcelas fueron instaladas antes del 2011• Alto costo y un largo proceso para determinar que productores podrían cumplir con este año, ante la falta de imágenes satélite de alta resolución a la escala necesaria.• Al excluir a la gran mayoría de productores, los compromisos se convertirían en pilotos más que catalizadores para un cambio transformacional a gran escala• Excluye a productores que no puedan demostrar que sus parcelas fueron instaladas antes del 2014• Alto costo y largo proceso para determinar qué productores podrían cumplir con este año, ante la falta de imágenes satélite de alta resolución a la escala necesaria• Al excluir a la gran mayoría de productores, los compromisos se convertirían en pilotos, más que en catalizadores para un cambio transformacional• Referencia sólo conocida por algunos productores de palma. Año de referencia para RSPO para Perú en negociación• Excluye a productores que no puedan demostrar que sus parcelas fueron instaladas antes del 2017• Alto costo y largo proceso para determinar que productores podrían cumplir con este año, ante la falta de imágenes satélite de alta resolución a la escala necesaria• Al excluir a la gran mayoría de productores, los compromisos se convertirían en pilotos, más que catalizadores para un cambio transformacional a gran escala • Información general del productor o productora, del cultivo (nombre científico y nombre común), volumen de producción, coordenadas georreferenciadas de la parcela,• Propiedad o cesión en uso formalizada (en propiedad, con contrato de cesión en uso o constancia de posesión) con perímetro y zonificación interna georreferenciada• Información general• Para el caso de empresas: trazabilidad y registro de productores y productoras, que incluyan el área total cultivada y especificaciones técnicas del cultivo. Esto puede ser a través de padrones de cooperativas y asociaciones agrarias, el padrón agrario de MIDAGRI o mecanismos privados implementados por empresas u organizaciones de soporte• Que no se encuentre en áreas superpuestas para solicitud de titulación de comunidades nativas. Estas podrán ser incluidas, una vez se resuelvan los conflictos• Que los interesados no cuenten con denuncias penales o procesos administrativos por deforestación, cambio de uso de la tierra, tenencia ilegal de la tierra, corrupción, presentación de información falsa o similares• Universo potencial: 223.738 productores de café, 89.789 productores de cacao, 3.185 productores de palma aceitera y un número desconocido de productores de ganadería bovina• Monitoreo de los compromisos de cafetaleros, cuyas áreas de producción bajo sombra se confunden con bosques, aun con imágenes de alta resolución. Estas requieren complementarse con imágenes de radar o validación en campo• Para poder monitorear los compromisos de producción libre de deforestación se requiere contar con el perímetro total de las parcelas (tituladas o no) georreferenciadas y realizar una zonificación interna, que defina el área de bosques a conservar. Se recomienda además realizar un análisis de riesgos de posibles causas externas de deforestación (p.ej. invasiones, presencia de economías ilegales, entre otras)• Se requiere un registro de todos los productores y productoras, al igual que lo que se desea incluir. Similar a esto está la propuesta del padrón agrario promovido por MIDAGRI (en la actualidad, lleva registrado más de 100.000 productores), los padrones de las cooperativas y asociaciones agrarias amazónicas, asimismo, los registros de las empresas que implementan proyectos en asociación con pequeños productores• Gran porcentaje de los productores no están asociados y no cuentan con propiedad titulada (alrededor del 70 u 80%, a excepción en la cadena de palma aceitera, donde la formalización es mayor), por lo que conseguir la información georreferenciada implica un esfuerzo adicional• Es necesario definir los incentivos para conservación de los bosques remanentes o colindantes a conservar. Por sí solo, el reconocimiento no será suficiente• Riesgo reputacional y falsa percepción de \"green wash\"• Ampliar año de referencia de agricultura libre de deforestación como actividad elegible REDD+, según las salvaguardas abajo descritas• Actualizar el año de referencia para los Contratos de Cesión en Uso para Sistemas Agroforestales (CCUSAF)• Excluir a personas naturales y jurídicas que cuenten con procesos administrativos o penales en curso o con sentencia firme por deforestación, cambio de uso de la tierra, presentación de información falsa, corrupción de funcionarios similares• Incluir compromisos voluntarios de respeto a los derechos colectivos de los pueblos indígenas y para la implementación de buenas prácticas de transparencia y anticorrupción• En el caso de deforestación legal considerar criterios de compensación• Tener en cuenta la gradualidad y la mejora progresiva con diferentes niveles de compromiso (por ejemplo, el sistema de estrellas de la huella de carbono)La propuesta año de referencia debe permitir la incorporación a una mayor cantidad de productores y, por lo tanto, a los bosques remanentes en sus parcelas y áreas no categorizadas colindantes, bajo compromisos de conservación de bosques. Por ello, una propuesta de 2020 que es la más factible, desde el punto de vista de la capacidad técnica, y es consistente con las propuestas de regulaciones internacionales sobre deforestación importada, debe observarse como una mirada realista y una oportunidad para conservar los 771.854,49 hectáreas de bosques tropicales amazónicos en predios privados, y reducir la probabilidad de deforestación en 13.488.061,05 hectáreas de bosques tropicales, en tierras sin categoría legal asignada. a generar condiciones habilitantes (sistemas de monitoreo, promoción de la trazabilidad, pilotos de plataformas de servicios y diseño de incentivos, entre otros), para escalar este modelo de negocio libre de deforestación, de acuerdo con las demandas futuras del mercado internacional y al aportar al cumplimiento de los compromisos internacionales que se tienen como país.• La Alianza en el marco del proyecto SAB, como parte de la CPS, ha facilitado el proceso de definición de año de referencia, donde el 31 de diciembre de 2020 es el año preferido por los actores consultados. Los principales argumentos para esta selección son los siguientes: a) cuenta con mayor disponibilidad de información satelital, lo cual facilita el monitoreo de los compromisos de producción libre de deforestación, a escala de parcelas a un menor costo y mayor eficiencia; b) es aceptado por la comunidad internacional para fines comerciales y no es incompatible con los compromisos internacionales asumidos por el Gobierno peruano, en materia de bosques y cambio climático; c) brinda la posibilidad de incluir mayor cantidad de productores y productoras y, por lo tanto, conseguir mayores compromisos para la conservación de los bosques y la producción libre de deforestación; por último, d) por la antigüedad de los cultivos (palma aceitera, 3 años; cacao, 3-4 años y café, 3-4 años), incluye a todas las áreas actuales bajo producción.• La aprobación de las regulaciones internacionales de deforestación importada generará un riesgo destacado en el acceso al mercado de las principales cadenas productivas (café, cacao, palma aceitera y ganadería) de la Amazonía peruana, toda vez que estas cadenas -y las jurisdicciones asociadas -no cuentan con incentivos para promover la transición hacia la sostenibilidad y producción sin deforestación. Así mismo, todavía no se han construido mecanismos y herramientas de monitoreo y trazabilidad, que permitan demostrar que la producción no está asociada a la deforestación. Además, este nuevo requerimiento puede convertirse en una oportunidad para diferenciarse en los mercados internacionales.• Sobre la base del Sistema Integrado de Estadísticas Agrarias del MIDAGRI, un análisis especializado estima que el impacto de estas regulaciones podrá afectar a más de 223.738 productores de café, 89.789 productores de cacao, 3.185 productores de palma aceitera y un número desconocido de productores de ganadería bovina. Del mismo modo, puede tener un impacto económico anual de al menos USD 925,8 millones (USD 646 millones de exportación de café en 2020 y USD 279,8 millones de exportación de cacao en 2020).• En ese sentido, los compromisos voluntarios de producción libre deforestación para las cadenas de cacao, café, palma aceitera y ganadería -que promueve la CPS y proyectos que contribuyen a esta como el proyecto IKI SAB -pueden ayudarPróximos pasos• Si bien existe una base importante y representativa de actores que estaría de acuerdo con el 31 de diciembre del 2020 como año de referencia, se requiere continuar con los diálogos para fortalecer el consenso generado. En particular, esto es relevante a nivel subnacional y local, donde es necesario informar y retroalimentar la propuesta del año de referencia.• Aunque el año de referencia está vinculado a compromisos voluntarios, se debe armonizar con las regulaciones nacionales en los ámbitos que corresponda o aplique.• Es necesario involucrar a nuevos actores nacionales, como el Ministerio de Comercio Exterior y Turismo, al igual que al Ministerio de Relaciones Exteriores, para promover y articular los compromisos por la libre deforestación de la estrategia exportadora y la competitividad de mercado de las exportaciones de agricultura tropical en el Perú.• Al igual que con el Acuerdo Cacao, Bosques y Diversidad, procesos similares deben desarrollarse con la palma, el café y la ganadería. De este modo, se establecerán las acciones y los mecanismos para eliminar la deforestación, con base en el año de referencia propuesto. La CPS puede ser la plataforma sobre la cual se anidan y se diseñan estos compromisos.• Es necesario seguir avanzando sobre esquemas de monitoreo para productos agropecuarios libres de deforestación. El proyecto SAB avanzó con un prototipo aplicado a dos modelos de negocios en cacao y palma. Este prototipo puede ser validado con otros actores de la CPS y aplicado a nuevos modelos de negocios.• Las lecciones aprendidas en el desarrollo de los pilotos de modelos de negocios cero deforestación del proyecto SAB deben traducirse en acciones que permitan el desarrollo de nuevos modelos en el marco de la CPS.","tokenCount":"4318"} \ No newline at end of file diff --git a/data/part_6/1369570644.json b/data/part_6/1369570644.json new file mode 100644 index 0000000000000000000000000000000000000000..64bae4e7ac102ffe4e54936adae4508e7b437fbc --- /dev/null +++ b/data/part_6/1369570644.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"b03ff8853755ff7ace465aeb90b9a275","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/8f204910-4e4d-42c6-9f4e-6f9ed4b90286/retrieve","id":"-1937877478"},"keywords":[],"sieverID":"860f25ad-a39c-41b3-8948-30e0aacdce91","pagecount":"1","content":"Providing a variety of fodder options improves the nutritional content of feed, promoting healthier livestock and reducing the need for supplemental inputs. ▪ Key message 2. Climate Resilience: Planting diverse forages contribute to climate resilience by sequestering carbon, mitigating greenhouse gas emissions, and providing buffers against extreme weather events.▪ Key message 3. Community Engagement: Cultivating fodder diversity fosters community involvement in sustainable land management practices, promoting local knowledge sharing, and fostering a sense of stewardship over natural resources.In western region, conventional farming practices have led to monocultures, habitat destruction, soil degradation, and loss of biodiversity. These practices not only compromise the longterm health of ecosystems but also jeopardize the livelihoods of farmers and the resilience of food systems. In response to these challenges, there's a growing recognition of the importance of transitioning towards nature-positive approaches in agriculture. This approach aims to restore and enhance the health of ecosystems while simultaneously supporting human well-being. Diversifying fodder cultivation fits within this framework as it offers a multifaceted solution.Agroforestry Systems: Integrating fodder trees within agroforestry systems offers multiple benefits, such as enhancing biodiversity, improving soil health, and providing additional income streams for farmers. Fodder Crop Diversification: Promoting the cultivation of diverse forages species, including grasses, legumes, and fodder trees, enhances ecosystem resilience, supports pollinators and beneficial insects, and improves livestock nutrition. Community-Based Fodder seed Banks: Establishing community-managed fodder banks enables farmers to access a diverse range of fodder species, seeds, and planting materials, thereby promoting fodder diversity and resilience at the local level. ","tokenCount":"250"} \ No newline at end of file diff --git a/data/part_6/1383923210.json b/data/part_6/1383923210.json new file mode 100644 index 0000000000000000000000000000000000000000..9c863e3ecb01018b9704c040fdc7ab1ded68a42a --- /dev/null +++ b/data/part_6/1383923210.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"82c0192fc277ee4412dc77a08ab0680b","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/c317d4e6-37ea-41e5-99db-30c873338161/retrieve","id":"1742308087"},"keywords":[],"sieverID":"ab812a70-c568-4ca1-9fc3-5a7338aaeb49","pagecount":"13","content":"Peste des petits ruminants (PPR) demeure toujours l'une des principales causes de mortalité élevée du bétail en région sahélienne, avec pour conséquence des risques de fragilisation des systèmes pastoraux, baisse de revenu des éleveurs, insécurité alimentaire des ménages et les questions de santé publique. Présente dans plus de 70 pays et endémique particulièrement en Afrique de l'Ouest, la PPR est une maladie virale, à transmission directe, affectant majoritairement les caprins et les ovins. La PPR peut infecter jusqu'à 90% d'un troupeau et en tuer entre 30% et 90% des animaux infectés, avec des retombées importantes sur le revenu des éleveurs et l'économie nationale. Depuis 2015, la PPR fait l'objet d'une campagne mondiale d'éradication à l'horizon 2030 (OIE and FAO 2015).Le projet Epidémiologie et Contrôle de la Peste des Petits Ruminants (ECo-PPR) financé par l'International Fund For Agricultural Develpment (IFAD) et mise en application par l'International Livestock Research Insitute (ILRI) s'est donné pour objective d'informer et de soutenir les efforts en cours aux niveaux national, régional et mondial pour le contrôle et l'éradication de la PPR en générant les preuves nécessaires pour soutenir son dialogue politique (ILRI 2019). Spécifiquement, le projet ECo-PPR vise à combler les lacunes existantes en épidémiologie et mieux estimer l'impact socio-économique de la maladie. Ce dernier est mis en place dans six pays différents, dont trois en Afrique de l'Est (Tanzanie, Kenya, Éthiopie) et également trois en Afrique de l'Ouest (Sénégal, Mali, Burkina Faso).Dans ce contexte, une équipe de chercheurs de l'ILRI basée à Dakar, Sénégal organise une série d'ateliers de modélisation participatives avec diffèrent groupe d'acteurs intervenant dans la chaine de valeur des petits ruminants au Sénégal. L'objectif de ces ateliers participatifs est de rassembler les acteurs clés (hommes et femmes) comme éleveurs, commerçants, agents vétérinaires, les responsables administratifs locaux intervenant dans la vaccination et la santé animale pour mieux comprendre comment les pratiques courantes d'élevage influencent la propagation de la PPR. La finalité de ces ateliers est de concevoir ensemble des stratégies de contrôle de la PPR adaptées aux réalités locales ; et d'évaluer l'impact économique de la PPR.Pour atteindre lesdits objectifs, l'equipe de l'ILRI utilise la méthode dénommée Spatial Group Model Building (SGMB) qui a termes, conduira à l'élaboration d'un modèle de système dynamique quantitatif relativement complexe permettant d'évaluer l'impact socioéconomique de la PPR. Ces activités de modélisation participative se déroulent sur deux sites avec chacun un système agricoles distinct. Il s'agit des départements de Linguère au nord du Sénégal, dans la zone semi-aride où l'élevage extensif est prédominant ; et Koungheul dans la zone centrale qui est essentiellement agropastorale.Pour la mise en oeuvre de ces ateliers SGMB, les chercheurs de l'ILRI ont mis en place au niveau de chaque site d'étude d'une équipe de facilitatation localement recruté pour assister ces derniers sur le terrain.La formation de l'équipe de faciliation sur l'approche SGMB a pour objectif de doter les participants des connaissances et compétences nécessaires à la construction des modèles de groupe spatiaux avec les acteurs clés des chaines de valeur, et leur applications dans divers domaines, notamment dans celui de l'élevage des petits ruminants. Cette formation leurs a aussi permis aussi d'avoir une meilleure compréhension de l'approche systémique en général.Le renforcement des capacités du personnel de terrain est un catalyseur essentiel à la réalisation des résultats de recherche et des impacts du développement. Il est aussi intégré dans la stratégie CRP de l'élevage, mais également au niveau des théories du changement et des cannaux d'impacts indiquer par l'ILRI (Dror et al. 2019).La formation a durée cinq jours et est administré selon le programme présenté en Annexe. La plupart des materiels utlisés pour cette dernière sont accessible en ligne et présenté avec different focus, l'un sur l'approche systémique et l'autre sur la modelisation participative spatiale or SGMB (Rich 2020).Le premier jour, cette dernière a débuté par une séance de présentation des formateurs et des participants pour permettre aux uns et aux autres de mieux se connaitre. Ensuite, les formateurs ont procédé à une introduction à l'approche systémique et l'importance de l'utilisation de celle participative. Ainsi, de manière très détaillé le principal vocabulaire 1 de la méthode SGMB a été abordé avec plusieurs exemples illustratifs permettant aux participants de mieux les comprendre.Le deuxième jour, les principes de construction de modèle de groupe (GMB) et ceux de construction de modèle de groupe spatial (SGMB), ont été abordés. Cette session a permis aux participants d'avoir une meilleure compréhension du processus de construction de modèle de groupe spatial et les contextes dans lesquels ils peuvent être utiliser. En plus, une étude de cas pratique à travers l'article de Mumba et al. ( 2017) qui traite de la Fièvre de la Côte Est en Zambie en utlisant la méthode SGMB, a été effectuée.Le troisième jour, chacun des participants a fait une présentation à la fois descriptive et critique de l'étude de cas de la Zambie, suivi de l'appréciation de la formatrice qui a jugé nécessaire de reconduire l'exercice pour une journée additionnelle incitant les participants à mieux approfondir leurs connaissances des divers outils appris. Néanmoins, la deuxième partie des principes de construction de modèle de groupe spatial a été abordé. Après cela, il s'en est suivi la partie basée sur les planifications des sessions SGMB et de leurs agendas.• Planification : different scripts et leur processus de mise en oeuvre 1 Stock, flux, convertisseur, feedback etc.• Agendas : une suite d'activité pré-etablit qui décrit de manière détaillé les sessions de SGMB afin qu'elles soient méticuleusement préparées. Le cinquième et dernier jour de la formation était consacré à la phase de simulation et de terrain. Ce dernier a eu lieu au niveau de la commune de Sangalkam avec différents acteurs de la chaine de valeur des petits ruminants, c'est-à-dire, producteur, transformateur, commerçant et vétérinaire. La simulation s'est déroulée dans la salle de réunion de l'Unité de Production des Plants Fruitiers de l'ISRA basée à Sangalkam, entre neuf heures et midi. Après une séance de présentation des différents participants, le script 0 a été réalisé, à savoir la présentation du projet ECo-PPR, la présentation de l'activité de modélisation participative et des résultats attendus. Après cela, le script 1 a été réalisé, notamment en ce qui concerne l'explication du vocabulaire de la méthode SGMB, l'identification et la hiérarchisation des problèmes liés à la PPR dans la zone et l'identification des causes et des conséquences. Enfin, le script 3 a été aussi réalisé concernant l'utilisation de la carte (Layerstack) pour localiser les principales infrastructures pastorales de la zone telles que les points d'eau, les marchés à bétail, les zones de pâturage et les postes vétérinaires publiques et privées sur la carte de la zone. Malheureusement, le temps imparti n'a pas été assez suffisant pour permettre de procéder à la mise en relation des causes et des conséquences à savoir les feedbacks.A la fin de la simulation, une séance de débriefing a été effectuée sur place pour l'évaluation du test. L'importance de cette séance de débriefing est capitale car les points positifs et négatifs relevés à travers ce exercice vont guider et améliorer les compétences prochaines de l'ensemble de l'equipe pour les étapes futures de la modélisation participative.Les participants ont tous montré leur engouement à prendre part à la simulation et ont essayé d'apporter les meilleures réponses possibles. Chacun d'eux a essayé d'apporter des réponses par rapport au maillon de la chaine de valeur dans laquelle il se situe. Ainsi, les trois scripts testés de la méthode SGMB ont été exécutés tant bien que mal.Une pause-café a été observée pour permettre aux participants de souffler un peu et prendre plus de force pour la suite. En rapport à la situation du Covid 19, les mesures de protection individuelles et collectives ont tous été respectées. A la fin du test, les participants ont bénéficié d'un remboursement de transport à hauteur de 2500 FCFA par personne et ont été chaleureusement remerciés par l'équipe de recherche.Durant la simulation, certains téléphones portables ont plusieurs fois sonné, perturbant ainsi la séance. Au début du test, certains participants ont soutenu que la PPR n'était pas un problème majeur dans leur zone. Ce qui laisse penser que cette zone ne constitue pas le meilleur choix pour faire cette simulation. Toutefois, lorsque quelques participants ont commencé à donner des exemples de petits ruminants atteints par la PPR et les désastreuses conséquences que cela entraine dans leurs troupeaux, les autres ont reconnu le problème et ont commencé à participer au débat.Au début, les questions étaient trop générales et semblaient sortir du contexte de la PPR en ce sens que les participants ont surtout exposé des problèmes liés à l'élevage en général tels que l'accès à l'aliment de bétail, aux pâturages, à l'eau et au problème lié au vol de bétail. Cela est surtout dû à la manière dont la première question relative au probleme principal auxquels les participants sont confrontes a été posée. En effet, cette dernière n'était pas spécifiquement relative à la PPR ce qui causa une distraction passagere des participants. Heureusement le débat a été recentré sur la PPR et les causes et conséquences de celle-ci ont été largement abordées.Il est préférable de procéder d'abord à une présentation PowerPoint des concepts et vocabulaires de la méthode SGMB pour permettre aux participants de mieux comprendre et saisir plus facilement la différence entre les concepts.Concernant la cartographie de la zone, il serait bien de dresser des symboles pour chacune des infrastructures pastorales. Ainsi, ces symboles seront adoptés lors des prochaines séances afin de faciliter la représentation des infrastructures pastorales sur la carte. Cela évitera de représenter une même infrastructure pastorale de plusieurs manières dans différentes cartes.Les deux facilitateurs ou participants de la formation sur l'approche systémique et la modelisation participative, SGMB, se sont montrés très engagés à apprendre et dévoués à leurs diverses tâches respectives. Les séances de formation théorique sur le SGMB au siège de l'ILRI et celle pratique à Sangalkam ont permis d'identifier aussi bien leurs points positifs que ceux qui nécessiteraient davantage d'améliorations avant les sessions de modélisations participatives de Linguère, Sénégal.Sur le terrain, les facilitateurs ont mis un peu de temps à se mettre dans le vif du sujet et à prendre des initiatives personnelles allant dans leur tâches respectives. Mais après quelque temps, ils ont tous retrouvé leurs marques et étaient très operationels. Néanmoins, leurs aptitudes à diriger des sessions de SGMB demeurent assez limitées. En tant que formatrice et investigatrice principale de cette activité de modélisation participative, j'approuve aussi leur suggestion de faire une ou deux autres tests de simulations pour leur permettre ainsi qu'à nous, équipe de recherche, de développer de meilleures aptitudes pratiques de terrain ainsi qu'une synergie de groupe nécessaire pour la bonne marche des ateliers de SGMB à Linguère.Néanmoins, des séances d'apprentissage en ligne sont prévues toujours dans le but d'approfondir et de mieux capitaliser les connaissances et aptitudes tirées lors de la formation de même que les enseignements tirés de la simulation de terrain.Je remercie une fois encore chacun des deux facilitateurs pour leurs dévouements et espère qu'ils vont continuer à s'améliorer. J'espere les revoir très bientôt pour une autre phase de simulation de terrain. ","tokenCount":"1843"} \ No newline at end of file diff --git a/data/part_6/1394167940.json b/data/part_6/1394167940.json new file mode 100644 index 0000000000000000000000000000000000000000..4f22759021f528244d25da0b4d7c51a0db35d4b5 --- /dev/null +++ b/data/part_6/1394167940.json @@ -0,0 +1 @@ +{"metadata":{"gardian_id":"7a8f4bfbeb7113653e539e9ec21c68bf","source":"gardian_index","url":"https://cgspace.cgiar.org/rest/bitstreams/02784fa0-02a9-4b0d-9ea7-84e2e3f67531/retrieve","id":"-332204302"},"keywords":[],"sieverID":"a404b260-fda7-4b03-b913-41c31ebd9ed7","pagecount":"57","content":"The International Institute of Tropical Agriculture (IITA) was founded in 1967 as an international agricultural research institute with a mandate for improving food production in the humid tropics and to develop sustainable production systems. It became the first African link in the worldwide network of agricultural research centers known as the Consultative Group on International Agricultural Research (CGIAR), formed in 1971. IITA is governed by an international board of trustees and is staffed by approximately 80 scientists and other professionals from over 30 countries, and approximately 1,300 support staff. Staff are located at the Ibadan campus, and also at stations in other parts of Nigeria, and in Benin, Cameroon, Cote d'Ivoire, and Uganda. Others are located at work sites in several countries throughout sub-Saharan Africa.Financial support for the research agenda of IITA is provided by the Governments of Austria,Agricultural Development, Rockefeller Foundation, Sasakawa Africa Association, United Nations Development Programme, the World Bank and others.IITA's mission is to enhance the food security, income and well-being of resource-poor people primarily in the humid and subhumid zones of sub-Saharan Africa by conducting research and related activities to increase agricultural production, improve food systems, and sustainably manage natural resources, in partnership with national and international stakeholders. To this end, IITA conducts research, germplasm conservation, training and information exchange activities in partnership with regional bodies and national programs including universities, non-governmental organizations (NGOs) and the private sector. The research agenda addresses crop improvement, plant health, and resource and crop management within a food systems framework and is targeted at the identified needs of three major agro-ecological zones: the savannahs, the humid forests, and the mid-altitudes.Research focuses on smallholder cropping and post-harvest systems and on the following food crops: cassava, cowpea, maize, plantain and banana, soybean and yam. In addition to collecting and preserving the germplasm of these crops, IITA has also collected germplasm of bambara groundnut and now has about 2000 accessions of this species in its genebank. This collection has been partially characterized and documented and the germplasm is available to researchers worldwide.The International Bambara Groundnut Network (BAMNET) was founded as a result of an International Bambara Groundnut Workshop, held [14][15][16] November 1995 in Harare, Zimbabwe. Bambara groundnut is indigenous to Africa. It is an under-utilized crop and neglected by research. The genetic resources of this crop face a serious threat of genetic erosion in farmers' fields. The objectives of BAMNET are to increase the importance of bambara groundnut by improving its productivity, production, marketing and consumption. BAMNET has more than 120 members from about 28 countries (18 African, 8 European, Israel, USA), FAO and IPGRI. Activities of BAMNET encompass aspects such as agronomy, germplasm conservation and management, breeding, utilization, information, documentation, economics and sociology. However, at present, the activities of BAMNET will focus on crop improvement and breeding; processing and marketing; and information and communication.Citation IPGRI, IITA, BAMNET. 2000. Descriptors for bambara groundnut (Vigna subterranea). International Plant Genetic Resources Institute, Rome, Italy; International Institute of Tropical Agriculture, Ibadan, Nigeria; The International Bambara Groundnut Network, Germany. IPGRI encourages the use of material from this publication for educational or other noncommercial purposes without prior permission from the copyright holder. Acknowledgement of IPGRI's material is required. This publication is available to download in portable document format from URL: