text: Hemoglobin A in humans can form hemichromes even under physiological conditions as a result of pH and temperature alterations, and the autoxidation of oxyhemoglobin. Hemichrome formation, followed by a band 3 clustering and the formation of Heinz bodies, can take place during the physiological clearance of damaged red blood cells. The difference between a normal red blood cell (RBC) and a red blood cell with unstable hemoglobin (such as in the case of hemolytic anaemia) is that, in a normal RBC, the formation of Heinz bodies is significantly delayed. In cells with unstable hemoglobin, hemichromes are formed soon after the cell has been released into the bloodstream and they precipitate on the membrane's surface.