text: The production of natural products is regulated by the biosynthetic gene clusters (BGCs) encoded in the microorganism. By adopting genome mining, the BGCs that produce the target natural product can be predicted. Some important enzymes responsible for the formation of natural products are polyketide synthases (PKS), non-ribosomal peptide synthases (NRPS), ribosomally and post-translationally modified peptides (RiPPs), and terpenoids, and many more. Mining for enzymes, researchers can figure out the classes that BGCs encode and compare target gene clusters to known gene clusters. To verify the relation between the BGCs and natural products, the target BGCs can be expressed by suitable host through the use of molecular cloning.