text: This pathway has been demonstrated in Chloroflexus, a nonsulfur photosynthetic bacterium; however, other studies suggest that 3-hydroxypropionate bicycle is used by several chemotrophic archaea. T In E. coli 3-hydroxypropionate bicycle has been studied and found to be insensitive to oxygen. This means that within the pathways there is nothing that oxygen can affect because in either part of the pathway or the oxygen is used to drive the reaction forward.