text: Bacteriochlorophyll and carotenoids are two molecules responsible for harvesting light energy. Current models of the organization of bacteriochlorophyll and carotenoids (the main constituents) inside the chlorosomes have put them in a lamellar organization, where the long farnesol tails of the bacteriochlorophyll intermix with carotenoids and each other, forming a structure resembling a lipid multilayer.
  Recently, another study has determined the organization of the bacteriochlorophyll molecules in green sulfur bacteria. Because they have been so difficult to study, the chlorosomes in green sulfur bacteria are the last class of light-harvesting complexes to be characterized structurally by scientists. Each individual chlorosome has a unique organization and this variability in composition had prevented scientists from using X-ray crystallography to characterize the internal structure. To get around this problem, the team used a combination of different experimental approaches. Genetic techniques to create a mutant bacterium with a more regular internal structure, cryo-electron microscopy to identify the larger distance constraints for the chlorosome, solid-state nuclear magnetic resonance (NMR) spectroscopy to determine the structure of the chlorosome's component chlorophyll molecules, and modeling to bring together all of the pieces and create a final picture of the chlorosome.
  To create the mutant, three genes were inactivated that green sulfur bacteria acquired late in their evolution.  In this way it was possible to go backward in evolutionary time to an intermediate state with much less variable and better ordered chlorosome organelles than the wild-type. The chlorosomes were isolated from the mutant and the wild-type forms of the bacteria. Cryo-electron microscopy was used to take pictures of the chlorosomes. The images reveal that the chlorophyll molecules inside chlorosomes have a nanotube shape. The team then used MAS NMR spectroscopy to resolve the microscopic arrangement of chlorophyll inside the chlorosome. With distance constraints and DFT ring current analyses, the organization was found to consist of unique syn-anti monomer stacking. The combination of NMR, cryo-electron microscopy and modeling enabled the scientists to determine that the chlorophyll molecules in green sulfur bacteria are arranged in helices.  In the mutant bacteria, the chlorophyll molecules are positioned at a nearly 90-degree angle in relation to the long axis of the nanotubes, whereas the angle is less steep in the wild-type organism. The structural framework can accommodate disorder to improve the biological light harvesting function, which implies that a less ordered structure has a better performance.