text: Another FCS based approach to studying molecular interactions uses fluorescence resonance energy transfer (FRET) instead of fluorescence, and is called FRET-FCS. With FRET, there are two types of probes, as with FCCS; however, there is only one channel and light is only detected when the two probes are very close&mdash;close enough to ensure an interaction. The FRET signal is weaker than with fluorescence, but has the advantage that there is only signal during a reaction (aside from autofluorescence).