text: Reverse transcriptases (RTs) are polymerases capable of synthesizing complementary DNA (cDNA) using either RNA and DNA templates and have become essential biotechnology tools in both clinical and laboratory settings. OTTR makes use of a unique non-long terminal repeat (LTR) retroelement RT called BoMoC, due to its specialized ability to synthesize cDNA opposite templates containing modified bases or sugar backbones and being highly processive across discontinuous RNA templates. Originally purified from the silk moth Bombyx mori, OTTR BoMoC is N-terminally truncated and modified to introduce a stabilizing active site mutation.