text: Assuming a two state denaturation as stated above, one can derive the fundamental thermodynamic parameters namely, ,  and  provided one has knowledge on the  of the system under investigation.
  The thermodynamic observables of denaturation can be described by the following equations:
  where ,  and  indicate the enthalpy, entropy and Gibbs free energy of unfolding under a constant pH and pressure. The temperature,  is varied to probe the thermal stability of the system and  is the temperature at which half of the molecules in the system are unfolded. The last equation is known as the Gibbs–Helmholtz equation.