text: The study of DHS profiles combined with other techniques allows analysis of regulatory DNA in humans:
  *Transcription factor: Using the ChIP-Seq technique, the binding sites to DNA in certain transcription factor groups are determined, and the DHS profiles are compared.  The results confirm a high correlation, which show that the coordinated union of certain factors is implicated in the remodeling and accessibility of chromatin.
  *DNA methylation patterns: CpG methylation has been closely linked with transcriptional silencing.  This methylation causes a rearrangement of the chromatin, condensing and inactivating it transcriptionally.  Methylated CpG falling within DHSs impedes the association of transcription factor to DNA, inhibiting the accessibility of chromatin.  Data argue that methylation patterning paralleling cell-selective chromatin accessibility results from passive deposition after the vacation of transcription factors from regulatory DNA.
  *Promoter chromatin signature:  The H3K4me3 modification is related with transcriptional activity.  This modification takes place in adjacent nucleosome to the transcription start site (TSS), relaxing the chromatin structure.  This histone modification is used as a marker of promoters, using it to map these elements in the human genome.
  *Promoter/enhancer connections: distal cis-regulatory elements, such as enhancers are in charge of modulating the activity of the promoters.  In this way, the distal cis-regulatory elements are actively synchronized with their promoter in the cellular lines which is active the expression of the gene controlled.  Using the DHS profiles, were looked for correlations between DHS to identify promoter/enhancer connections.  Thus, it was able to create a map of candidate enhancers controlling specific genes.
  The data obtained were validated with the chromosome conformation capture carbon copy (5C) technique.  This technique is based in the physical association that exists between the promoter and the enhancers, determining the regions of chromatin that enter in contact in the promoter/enhancer connections.
  It was confirmed that the majority of promoters were related with more than one enhancer, which indicates the existence of a complicated network of regulation for the immense majority of genes.  Surprisingly, they also found that approximately half of the enhancers were found to be associated with more than one promoter.  This discovery shows that the human cis-regulatory system is much more complicated than initially thought.
  The number of distal cis-regulatory elements connected to a promoter is related to the quantitative average of the regulation complexity of a gene.  In this way, it was determined that human genes with more interactions with distal DHSs, and with at least one more complex regulation, corresponded with those genes with functions in the immune system.  This indicates that the complex of cellular and environmental signals processed by the immune system is directly encoded in the cis-regulatory architecture of its constituent genes.