In the previous section, we have described results from unbiased MD simulations and shown that the sampling may vary substantially on repeating a simulation. In this section, we include results from the biased simulation protocol named the "splitting protocol" which involves biasing the sampling of phase space towards sites of interest (described in detail in section 3). It should be noted that, in principle, such splitting steps can be continued further until the desired level of sampling has been achieved (for instance, if there is a substantial variation in the binding poses across sub-replicas that need to be explored further, and so on).