All strains examined in this study originated from Carlsbad thermal springs and were previously isolated and identified by methods described by Smrhova et al. The strains were reinoculated from UCTP collection glycerol stocks stored at -80 °C on TSA plates (1.7% Casein Peptone, 0.3% Soy Peptone, 0.25% Dextrose, 0.25% Dipotassium Phosphate, 0.5% NaCl solidified with 1.8% agar). The plates were then incubated at 37°C in the dark until visible growth was observed. The identity of the grown strains was verified by MALDI-TOF mass spectrometry measurement . Each strain was tested for notable growth in liquid TSB medium (TSA without agar) at the lowest and highest possible temperatures. Subsequently, the strains were cultivated under tested temperatures and 130 rpm in the dark. The strains were cultivated until visible growth was observed. In the case of the lowest growth temperature, this took up to 4 days. Cell cultures were centrifuged at 5,000 g for 5 minutes and the resulting pellets were lyophilized for 24 hours.