Even though there are significant differences in physicochemical parameters such as δ (20.7 to 47.85), V (18 to 106 cm3/mole), and V f (0.01 to 0.14), all total and fractional ∆H T m values stay constant. This again proves the correctness of Eq.31 that validates the . Based on this data, we calculated the fractions of the enthalpy responsible for specific mechanisms of DNA denaturation.