To test this new protocol, Papain-like protease protein (PL-pro) for SARS-CoV-2 coronavirus was used as a case study. 46 PL-pro is expressed during SARS-CoV-2 viral replication and strong evidence in support of its function suggests it as a promising target in combatting SARS-CoV-2 infection. Multiple crystal structures of SARS-Cov-2 PL-pro, co-crystallized with inhibitors are available on the Protein Data bank (Table ).