Our computational predictions could be tested with an experimental setup suitable to study single pore expansion. Accordingly, Khunlo et al. used single vesicle-to-supported bilayer fusion assay to dissect the role of α-synuclein in membrane fusion. Also, Nellikka Rohith et al. used an in vitro approach with v-SNARE-reconstituted nanodiscs and t-SNARE-reconstituted black lipid membrane to study α-synuclein modes of action in the micro-and milli-second time-scales. By replacing synuclein by synaptotagmin, these experimental setups could be applied to test our theoretical and computational results.