In conclusion, we found efficient non-aqueous interactions between the anticancer therapeutic leuprorelin (1) and ochratoxin A (OTA). The homogeneous interaction between 1 and OTA shows a binding constant of 10 6 M -1 in acetonitrile, while the fixation of 1 onto the surfaces of a mesoporous polymer resulted in stronger binding sites to OTA with a binding constant of 10 8 M -1 at 273 K. Thermodynamic analysis and DFT calculations revealed that 1:1 binding occurred between 1 and the mycotoxin even at the functionalized polymer interface, and this could facilitate the efficient extraction of OTA from food matrices, including fatty mixtures extracted from coffee beans, with improved reusability.