Briefly, 5 µL of 250 µM FAD and 5µl of 50mM D-Serine were added to all the wells in the assay ready plate (containing 250 nL compound / DMSO vehicle per well). 5 µL of a working solution containing 250 µM Amplex Red and 0.5 U/mL HRP was added to all the wells except for the negative control wells. 5 µL of 250 µM Amplex Red was added to the negative control wells containing 250 nL of DMSO vehicle. 10 µL of 5 µM H2O2 was added to all the wells. The plate was incubated for 10 minutes in the dark at 25˚C before measuring fluorescence in each well using the Envision plate reader with excitation at 530 nm and emission at 595 nm.