Lipids were annotated in MetaboScape 2021b (Bruker Daltonics) following the extraction of the four-dimensional data (m/z, ion mobility, intensity and spatial coordinate). Briefly, spectra of the entire measurement region per sample were exported from SCiLS Lab 2021c and imported into MetaboScape 2021b. Spectra from 10x10 pixel were averaged before feature extraction using the T-Rex 3 processing algorithm. An intensity threshold of 200 was used for peak detection and [M-H] -ions were selected as primary ion, seed ions were forbidden.