The amount of betamethasone 17-valerate added during manufacturing of the cream which is 0.122 g and the amount found by spectrophotometric analysis were subjected to statistical analysis to validate the results and investigate whether the discrepancy between the two values arise from systematic errors. The performed t-test indicated that the results are affected by indeterminate sources of errors. The proposed analytical method provides results with satisfactory degree of accuracy as it yielded a small margin of error of 1.63% and percentage recovery of 101.6 ± 0.003786 % w/w which shows that other excipients do not interfere with the analysis of the cream.