Search mode was configured to sample selected area diffraction as described in methods section 2.2, with a virtual camera length of 420-670 mm. In this mode, the Apollo was set to record single, 100frame movies per dataset. Target crystal locations were identified from the montage image, confirmed in 'Record' mode, and added to the navigator. A script (Supplementary Script 2) was then used to collect continuous rotation MicroED data from consecutive target locations, stored in the navigator.