Herein, we report the isolation, purification, and comprehensive characterization of corneal epithelial EVs (CE-EVs) as well as their loading in the aforesaid hydrogel for sustained release. A fraction of the encapsulated CE-EVs could be released from the hydrogel over ten days by diffusion through the hydrogel matrix, and the remaining can be released on demand through enzymatic degradation of the hydrogel. This represents a significant advancement in the field of corneal EV delivery. A perforation or epithelial wound healing would benefit from a moderate EV-release profile, while stromal regeneration after keratoplasty with a corneal implant would be supported by a very slow EV-release profile, occurring as the implant gets remodeled in vivo.