The LCC gene was cloned in pExp-Bla plasmid and transformed into Shuffled T7 express cells (New England BioLabs, catalogue number C3029J). The cells were grown at 37 °C in 1L of LB media containing 100 μg mL-1 ampicillin until the OD600nm reached 0.5 to 0.6. The expression of the recombinant protein was induced by adding 0.4 mM of IPTG at 20 °C for 20h.