Although short term experiments suggest that minimizing electrochemical scanning improves monolayer stability, longer term data reveals that for a monolayer stable in terms of intermolecular forces, scanning actually further improves monolayer stability by suppressing oxidation of alkylthiolate molecules (Figure ). Several papers have assessed the impact of electrochemical scanning on the degradation of aptamer sensors and have shown that loss of sensor redox tag current is dependent upon the number of scans performed and the width of the potential window used for interrogation. In order to assess the effects of electrochemical scanning in light of the thermal effects discussed in the previous sections, we additionally tested MCH and MCO sensors by performing scanning only once-a-day at either 4 ˚C or 37 ˚C. Tests were performed in both PBS (Figure ) and in serum (Figure ) with a surprising and similar conclusion for both media: longer term data over multiple days show that more frequent scanning is preferred as it can actually help further stabilize the monolayer. To illustrate this improved stability, we compared the voltammograms obtained by repeated scanning in PBS at 37 ˚C (Figure ) with those recorded once-a-day (Figure ). MCH sensors scanned oncea-day underwent rapid degradation such that they were unreadable after just 2 days. While the MCO sensors were able to survive for 7 days (Figure ), background currents increased significantly more than those repeatedly scanned in the same conditions (Figure ).