The relevant domains of synthetic lipopolysaccharide are: Acyl_transf_3 domain (Interpro entry: IPR002656) is the acyltransferase 3 domain. Acyl_transf_3 includes NolL protein, and NolL protein functions as an acetyltransferase . Acetyltransferase is an important enzyme in the process of lipid synthesis. The CTP_transf_like domain (Interpro entry: IPR004821) is a cytidine transferase-like domain. This protein family includes lipopolysaccharide core biosynthesis protein KdtB 116 . The Glyco_transf_8C domain (Interpro entry: IPR013645) is at the C-terminus of the glucosyltransferase and galactosyltransferase proteins. Glyco_transf_8C has lipopolysaccharide 3-α-galactosyltransferase activity, jointing the process of lipopolysaccharide biosynthesis. The Glycos_transf_1 domain (Interpro entry: IPR001296) is family 1 of glycosyltransferases. Glycos_transf_1 domain protein transfer UDP, ADP, GDP or CMP linked sugars to various substrates, including glycogen, fructose 6-phosphate, and lipopolysaccharide. Glycos_transf_1 can take part in various biosynthetic processes, including exopolysaccharide biosynthesis and lipopolysaccharide core biosynthesis 117 . PglL_A domain (Interpro entry: IPR031726) is a protein glycosylation ligase domain. PglL O-oligosaccharyltransferase differs from WaaL O-antigen ligases. PglL O-oligosaccharyl transferase (O-OTase) transfers oligosaccharides to serine or threonine in proteins . Glycos_transf_N domain (Interpro entry: IPR007507) is the N-terminus of 3-deoxy-D-manno-octulosonic-acid transferase. Glycos_transf_N can transfer activated sugars to various substrates, including glycogen, fructose 6-phosphate, and lipopolysaccharide 119 . Glycos_transf_N proteins with this domain transfer UDP, ADP, GDP, or CMP-linked sugars. A signal peptide flanks the N-terminus of this region. Rubredoxin_2 domain (Interpro entry: IPR041166) is LapB (lipopolysaccharide assembly protein B) 120 , rubredoxin metal-binding domain. The rubredoxin-like domain (Interpro entry: IPR024934) is a similar rubredoxin domain. The Wzz domain (Interpro entry: IPR003856, PFAM: PF02706) is the N-terminal domain of the polysaccharide chain length determinant. The chain length determining protein (or wzz protein) is involved in lipopolysaccharide (lps) biosynthesis, giving the O-antigen component of lps a modal distribution of chain length 121 .