Elevated PSA levels in the blood can serve as an indication of prostate cancer. Normal PSA levels in healthy men range up to 4 ng/ml with values exceeding this threshold raising concerns about potential prostate cancer . Serum PSA levels in females are generally considered negligible, however, breast tissues can also produce PSA. Elevated PSA levels in female serum may be indicative of conditions such as breast cancer, cysts, or fibroadenomas . Consequently, the detection of PSA in blood has become an increasingly prominent role in medicine, diagnostics, and scientific research.