The solution was removed, anti-αSMA primary antibody-Alexa Fluor 488 (1/1000, v/v, 0.5 µg/mL) diluted in 1% BSA was added, and the samples incubated at 4 ºC for 24 h. Then, the solution was removed, and the samples were washed three times with PBS for 5 min each. A solution of phalloidin-Alexa Fluor 647 in DMSO diluted in 1% BSA (1/300 v/v, 0.22 µM) and