To ensure that the introduction of new data does not cause extensive changes to the decision tree model, a new model was trained with the addition of 14 analytes to the initial 36 analytes in the training set. The new model obtained by training with all 50 analytes presented in this study is shown in Figure . The minimal changes in chemical features seen in this model indicate that the new model does not have many logical changes as compared to the previous model shown in Figure . The first three comparisons were the same between both the original 36-analyte model and the new 50-analyte model and the new model only introduced four additional functional groups. Three of these new functional groups were related to the nitro group present in the compounds in the new training set: 4-nitropyridine N-oxide and 4-nitroquinoline Noxide. Therefore, one can deduce that the model has added an additional comparison to prevent these compounds from being predicted as "reactive". As the number of protonated analytes with known reactivities towards MOP grows, this model could be retrained to incorporate these new analytes, yielding improved predictions in the future while retaining baseline performance and simplicity. Figure . The decision tree model obtained from retraining the model using the 70% cutoff and all 50 reactions (original 36 and 14 test reactions). This model is similar to the one obtained via a training set of 36 reactions but has an additional check for a nitro group which was not included in the original model. The lack of major change from model shown in Figure indicates that the final model is robust and is able to incorporate new functional groups.