It is important to note that linewidth parameters are added to provide best agreement with the experimental data in all sets of simulations. Experimentally, the observed linewidth depends on the experimental parameters including the pulse length used in the detection sequence. Here the linewidth broadening of simulated peaks is given as a Gaussian with a FWHM of 5 MHz which reproduces well all major experimental peaks. The linewidth parameter also encodes for the strain of hyperfine values, representing a variation across the molecular configurations present in the sample. It is possible that such variations in the open cubane structure provide larger strains in the hfcs of Mn3 and Mn4, thus reducing the observed amplitude of EDNMR signals from these couplings relative to Mn1 and Mn2. In the simulations of Chrysina et al., using the ENDOR algorithm, a linewidth for the anisotropic Mn4 coupling three times larger than that for any other coupling was used. Further supporting the open cubane model, the glycerol treated samples studied by Marchiori et al. show a similarly elevated D value to the methanol treated system, indicating that a similar perturbation occurs. In this study higher frequency measurements at 130 GHz (D-band) compared to 94 GHz (Wband) by Chrysina et al. , further raises the 55 Mn Larmor frequency, pushing the EDNMR transitions to higher frequency values and away from the central blind spot. However at higher fields, overlap of small hyperfine values with a 14 N spectral peak (υ 14 N = 17.536 MHz at 5.7 T) begins to occur. 2D-EDNMR spectroscopy may help to further aide in signal assignment of the overlapping signals of small magnitude transitions and 14 N transitions at higher fields.