In addition to the western blot analysis, we assessed the live cell kinetic degradation displayed by 86 in HiBiT-BRD4 HEK293 cells (Figure , Table ). Strikingly, 86 showed near equipotent degradation of BRD4 with the best heterotrivalent degrader 27, with a Dmax 50 value of 1.1 nM vs 0.6 nM. Interestingly, 86 was ~3-fold more potent than its trivalent counterpart 26, with a Dmax 50 value of 1.1 nM vs 3 nM (Table ).