Given the high number of consecutive sampling it is important to test if the sampling quality is consistent throughout the experiments. Fig. shows that, at each time point, the total area of all peaks is well conserved throughout, with a relative standard deviation of only 2.27% and 2.30%, respectively for samples number 3 and 4. Fig. shows that indeed stirring at different speeds leads to different rates of the exponential growth, borne by the established role of fibers and their breakage in this process . This is in agreement with previous observations. It is noted that at the elevated temperature experiments were performed here (40 °C), hexamer formation and replication speed are similar to the speed of BB oxidation, which would make it difficult to observe differences under different stirring rates. Therefore, experiments shown in Fig. were done by first pre-oxidation the building-block solution and then comparing 1000 with 200 rpm stirring rates (see experimental section).