In an input of either m1 or m2, the RNA droplets maintained the initial dense phase 3.5 h after the input (See also Movies S2 and S3). In the single-input reactions, the sensing X-motif allows only one strand displacement reaction, with the valency being conserved throughout the process (𝑓 = 4), as depicted in Figure . Thus, the initial network-like conformation does not yield macroscopic disassembly (Figure ), although the structural flexibility might become higher (Figure ). This increased flexibility is prone to the osmotic pressure from the surrounding phase, which was indicated by transient pore formation in the middle of the reaction for the input of m2 (Figure , Movie S3). In the inputs of m1 and/or m2 described above, we double-checked the samples after a one-day incubation at RT. In the inputs of both m1 and m2, the dissolved phase state did not yield unwanted recondensation, indicating that the network disassembly was conserved well. In the input of either m1 or m2, the final dense phase remained invariant (Figure ). These observations firmly suggest that the motif restructuring and resulting phase state were thermally irreversible.