Following the validation of our library, we established a classical molecular network that incorporated MS/MS data from the aligned oxPUFAs features (see all oxPUFA), serum samples, and algae samples, as illustrated in (Figure ). For the sake of reusability of oxPUFA data for the community, we chose classical molecular network workflow since FBMN would come short of utility in this case as the alignment procedure is machine/sample dependent. Our oxPUFA data, depicted by the blue nodes, exhibit strong connections to nodes exclusively found in the serum extracts (depicted in red) and algae (depicted in green), indicating that oxPUFA data is particularly effective in discerning other NEO-PUFAs as the ones already present in oxPUFA data (potentially originating from other fatty acids).