To date, ICG is the most common used clinical fluorophore, as it has an absorption peak maximum around 800nm and an emission peak maximum around 820nm, providing a higher signal-tobackground ratio due to lower autofluorescence and increased tissue penetration. Besides its preferable fluorescent characteristics, ICG's widely acceptance is also attributed to the confinement to the vascular compartment by binding with plasma proteins, fast and almost exclusive excretion into the bile, low toxicity, and safeness to be used with low incidence of allergic reactions. In the last few decades, ICG has been widely used in clinical research from sentinel lymph node (SLN) mapping, surgery guiding by intraoperative cholangiography of solid tumours/biliary tract/vascular flow, to accessing of anastomotic perfusion during reconstructive surgery.