To assess the behaviour of GlyR residues depending on ligand occupancy, root mean square fluctuations were calculated and reported in Figure , in particular for residues of the loops constituting the binding pockets and on the principal subunit. It was previously suggested that the highest levels of fluorescence at residue ALA68 on Loop 2 were directly linked to the ability of an agonist to bind and function. We found that when glycine is The variations in pocket properties which indicate their degree of contraction (e.g., water content, Loop C angle, and convex hull volume) may correlate with subunit movements.