The automated approach used here overcomes certain limitations by reducing the time required to prepare and execute the experiment while also providing for an opportunity to create the volume of samples per compound that were desired for this iteration of the SAMPL challenge. This approach extended into the chromatography analysis by way of an autosampler and a highthroughput sequence on the HPLC instrumentation for data collection. Because the experimental design was automated, the goal was to prepare samples that ensured the solute would go completely into solution, thereby avoiding the need for determining mass balance.