The previous findings were strategically employed to evaluate the potential of TBPBB as biomarkers for early screening and approximate estimation of pea seed yield in stressful environments. To this end, five pea seed varieties were cultivated in soil under four different stress conditions for 10 days. The fluorescence images of the germinated seed after 72 hours were listed in Fig. , the photos of sprouted plants at 10 th day were shown in Fig. and the planting-harvesting results were shown in Fig. . On the 10 th day, Vicia faba L showed varying reductions in embryonic root length under alkaline, drought, and heavy metal stress, with measurements of 10.31  1.17 cm, 9.93  1.73 cm, and 9.16  1.60 cm, respectively, compared to 11.63  1.66 cm in the blank control group (Fig. ). In contrast, under salt stress, the root length increased to 12.04  1.26 cm. Stem lengths under all stress conditions exceeded those of the blank control group. By the 10 th day, the germination percentage was 100% in the control and salt stress groups, while it decreased to 94.4% in metal stress group, 88.9% in the alkaline and drought stress groups. In comparison with Vicia faba L, the control groups of Sweet crispy, Pea sprout 2, ZW6, and ZW11 peas exhibited shorter roots and stems (Tables ).