To characterise the antagonist pharmacology of the synthesised compounds at both the A 2A and A 2B receptor, the ability to inhibit NECA induced cAMP accumulation was measured. Pharmacological characterisation of the compounds at the A 2B receptor, showed no inhibition of NECA induced cAMP accumulation at the concentrations tested. The previously mentioned Compound 7 and 9 caused inhibition of cAMP at the A 2A receptor, in the presence of NECA, further suggesting inverse agonism.