Although most ambient-based SCMS techniques allow for the analysis of live cells, they generally have relatively low throughput (e.g., 15 cells from nano-DESI MS , 32 cells from microprobe Capillary electrophoresis (CE)-ESI-MS , and 108 cells from the Single-probe SCMS ), largely due to necessary manual selection and analysis of individual cells. Because of the dynamic nature of cell metabolism, cell metabolites may vary during lengthy sample preparation and measurement. To preserve metabolomics features of live cells, researchers used quenching methods after cell isolation . Quenching can stop cellular metabolism and metabolomic transformations by lowing temperature (e.g., using liquid nitrogen (LN2) for snap freezing) or denaturing enzymes (e.g., adding organic solvents or acidic solutions) of cells. Quenching is pivotal to effectively arresting the cells' metabolic activities, encapsulating a momentary freeze-frame of its biochemical state . This is crucial for accurate metabolomic studies, in which capturing the precise temporal details of cellular metabolites is essential for understanding cellular function.