Then, two wounds were created on the back side of animals with a diameter of 10 mm after shaving them. Afterward, 20 µl of a 0.5 MaFarland S. aureus suspensions was employed for each wound to infect it. After 15 min, the related treatments were employed in each group. The wounds in each group were monitored and photographed at days 0, 3, 5, and 12. The images were analyzed by ImageJ software. on the 5 and 12th day, the wound and the surrounding repaired tissue were harvested and fixed in 4% paraformaldehyde. After dehydration with respectively: alcohol 70 %, 80%, 90%, absolute 1 and absolute 2, the samples were embedded in paraffin, section with 5-7 μm thickness were obtained by a microtome, finally, the samples were stained by H&E to investigate the wound repair and inflammation.