This work presents the standardization and benchmarking of a nanoindentation protocol for 3D printed elastomeric microstructures, addressing key challenges associated with adhesion forces at the tip-sample interface and substrate effects. The optimized nanoindentation method employs a conospherical indenter tip (r = 10.26 µm), a modified trapezoidal displacement profile lift-off segments at the start and end of the indentation, and the utilization of the nano-JKR model for data analysis, allowing for consistent elastic modulus determination of the 3D printed microstructures. Via thorough testing with four newly developed and readily accessible PDMSbased elastomer inks for 2PLP, the protocol effectively measured modulus ranging from 0.9 to 3