Although origami have been functionalized with numerous types of organic and inorganic moieties, methods that allow their site-specific attachment to the structure are still not trivial. Such guest molecules are frequently conjugated to oligonucleotides that are introduced before or during the assembly of the nanostructure. However, some molecules cannot tolerate the relatively harsh conditions required for origami folding, e.g., if the guest molecule is a protein, high annealing temperatures can lead to its denaturation. Moreover, the attachment of more than one guest molecule to the structure requires its conjugation to individual staples which can substantially increase cost. A different approach relies on the recruitment of conjugated oligonucleotides via Watson-Crick (W-C) hybridisation to single-stranded staple overhangs projected from the assembled nanostructure. However, such non-covalent interactions can result in stochastic dissociation of the oligonucleotides and loss of the introduced component.