Where indicated, starter cultures were supplemented with glucose (final concentration: 0.2% w/v). The next day, 10 mL of overnight culture was used to seed 500 mL of LB-KAN + (Halo-PKA) in 2L baffled flasks, then grown to OD600 = 0.5-0.6; at this point the temperature was lowered to 17 °C and protein expression was induced with IPTG (final concentration 0.5 mM).