Mixture A was prepared with varying amounts of salts ranging from 3.7 to 29.3 mg, totaling to 112.0 mg (Table ). The mixture composed of Sodium bicarbonate (14.4% v/v) (11.9% v/v). When this mixture was loaded to the microscope, several crystals appeared "melted" as their edges were not sharp suggesting that somehow in the process hydration may have occurred which may lead to a loss in crystal order and resolution. Hydration could have happened in the test tube prior to grid preparation. We also observed that several crystals clumped together so they were not suitable for analysis. Regardless of these concerns we selected 1961 crystals for autonomous diffraction and out of these only 913 (47%) delivered sufficiently good data for identification using unit cell parameters as discriminators (Table ). Since we also recorded the images of the crystals, we could go back and look at the morphologies of the crystals that did not diffract. Indeed, most of the problematic crystals had the issues described above suggesting that sample preparation should be improved in the future (Figure in Supplementary Information). Despite this concern we could identify all components in the mixture and even identify the constituent with lowest percentage. Calcium acetate monohydrate which was added at ~3.3% in the input was identified in 40 crystals corresponding to a 4.4% counting ratio (Figure ).