Validation of the compound by NA enzymatic activity assay. Eleven compounds were evaluated at a concentration of 100 μM, with Zanamivir as the control at 0.02 μM. 1:64 dilution of virus was selected for the assay. Data is normalized to the virus-only group, which is arbitrarily set to 100%. The graph summarizes n = 3 independent experiments. Error bars show mean ± SEM (unpaired two-tailed t-test). *p<0.1, **p < 0.01, ****p < 0.0001, ns = not significant. Comparisons between the virusonly group and compound 1, compound 7, and Zanamivir yielded p-values of 0.0146, 0.0079, and <0.0001, respectively. Structural insights from molecular docking and MD simulations.