According to the MD simulations, conformational flexibility was also observed in the protein. During the 200ns simulation, conformational changes in the loop region of the protein were observed. This shows that the loop opens up in the absence of glutamate to accommodate the glutamate in the cavity. This conformational change is further confirmed in the RMSF graph shown in Figure . Based on the RMSF graph, the peaks between residue numbers 580-600 show the conformational changes in the loop opening.