Notably, the proposed SAR model is inherently multifactorial. No single parameter, such as flexibility, backbone conformation type, side-chain packing density, or polar backbone accessibility, alone predicts hemolytic activity. Instead, the combination of these factors defines the activity profile. This SAR model offers a valuable framework for the rational design of related peptides or peptidomimetics, enabling the development of compounds with tailored pharmacological profiles. (Milwaukee, WI, USA). Ultra-pure water was purchased from the Milli-Q system (Temecula, CA, USA). MTS assay kit (98%) was purchased from Promega (Madison, WI, USA). Single donor human Plasma K2 EDTA was purchased from Innov-Research (Novi, MI, USA). All phospholipids and cholesterol were purchased from Avanti polar Lipids (Alabaster, USA).