Furthermore, even the MO entrapped GOx with no additional redox shuttle was previously reported to only maintain 100% activity for the first 5 days. An explanation for this prolonged stability is the Hofmeister effect induced by PF6 -within the water channel, which may lead to a reduction in the protein/aqueous interfacial tension, or a reduction in water channel size, either of which in turn could slower down the denaturing or unfolding process GOx. As shown in Figure , after 10 days the relative activity of Fc12-PF6/MO/GOx gradually decayed, reaching a reduction up to 80% after 20 days and up to 20% after 30 days of operation. show that the magnitude of the anodic peak current has a linear dependence with the applied voltage scan rates, which suggests that the mass transport of Fc12-PF6 is no longer under diffusional control.