Please do not adjust margins different stimuli (i.e., depolarization, immune response, etc.) and can readily participate in events such as coordination with amyloid-b, production of reactive oxygen species, protect against excitotoxicity, etc. . Thus, making measurements of free Cu(II) dynamics in a spatiotemporally resolved manner in vivo is essential to elucidate the role of Cu(II) in the etiology of AD and other neurodegenerative diseases. Fast scan cyclic voltammetry (FSCV) at carbon-fiber microelectrodes (CFMs) has been gaining traction as a technique with high spatiotemporal resolution capable of in situ measurements. It typically operates with 100ms time resolution and can measure from 10s-100s synaptic clefts simultaneously . One of the limitations of this method, however, is selectivity. Researchers have leveraged different approaches to improve selectivity for quantitation of different analytes (reviewed in Ou et al. ). Recent research has demonstrated that FSCV at CFMs is sensitive to metal ion reduction, including for Cu(II) . To improve selectivity for Cu(II) sensing, Hashemi and colleagues grafted Cu(II)-selective ionophores onto the CFM surface . This methodology yielded a highly selective Cu(II) sensor while maintaining good sensitivity. The limitation, however, is that it involved a threestep synthetic approach, including electrochemical deposition of diazonium moiety, protection of unreacted hydroxyl groups with silyl ethers, and ionophore grafting via Click chemistry.