Thanks to the integrated ChEMBL data, retrieving annotated compounds with reported activity against a specific target is possible. We applied this approach to retrieve the ChEMBL-reported anti-T. cruzi activities of compounds annotated in the Melochia umbellata [(Q6813281) (Malvaceae, Q156551)] extract we had evaluated as active against T. cruzi. For the 10 distinct returned compounds, we also queried the taxa in which they are reported from WD, returning 14 structure-organism pairs at the time of writing (Table , query 5). Interestingly, among these 10 distinct compounds annotated in the active M. umbellata extract, all are quinoline alkaloids reported in Waltheria indica (Malvaceae). The overall chemical similarity between these two taxa is confirmed at the experimental spectral level using the integration of features' peaks and neutral losses into ENPKG. For this we designed a SPARQL request that can be used to retrieve the features with the highest number of peaks and neutral losses in common with the MS 2 spectrum of the [M+H] + ion of waltherione G (Q110090875) detected in the Waltheria indica (Q7966688) aerial parts extract profiled in 2014 using another LC method and another Orbitrap spectrometer (Table , query 6) . This query reveals that the feature sharing the highest number of peaks and losses among those from the set of 1,940 PI analyses is feature id 1 in the PI mode analysis of the active extract of Melochia umbellata (with a confirmed cosine similarity of 0.98). This feature shares 20 peaks and neutral losses with the waltherione G [M+H] + ion, and the fasst search against the GNPS libraries further points towards a strong spectral match with waltherione G. Together, the previous SPARQL queries allowed us to putatively identify the compound(s) responsible for the observed anti-trypanosomatid activity of M.