In 2024, Luo, S., et al. established "a benchmark glycan profle for commercial therapeutic monoclonal antibodies (mAbs) approved by the US Food and Drug Administration (FDA)". The authors improved mAb production by analyzing glycosylation data from over 150 mAbs produced by various mammalian cell expression systems. Their main findings were that there are nine prevalent glycan epitopes, including N-acetylglucosamine, core fucose, terminal galactose, and several others -making up the benchmark glycan profile to aid in mAb biopharmacuetical development, quality control, and regulatory compliance in the industry . Paper 01 manuscript author: 86 word summary, Time: 5 minutes 31.66 seconds In February 2024, Fux, A., et al. established and compared the selection and production of lipopolysaccharide "(LPS)specific, monoclonal, human single-chain variable fragments (scFvs)" through yeast and phage display methods. The scFvs were fused to a human constant fragment crystallizable (Fc) conjugated to polystyrene microbeads. LPS endotoxin removal capacity was based on the functionalized bead binding properties. Five different scFvs were selected that had binding properties similar to a commercial polyclonal antibody, with two of the conjugated scFv-Fcs outperforming the commercial antibody, with 265x less endotoxins remaining in solution . Paper 02 manuscript author: 90 word summary, Time: 10 minutes 9.95 seconds In March 2024, Wang, X., et al. improved quality and productivity of an antibody-based biotherapeutic through the implementation of inverted frustoconical shaking bioreactors (IFSB) over stirred tank bioreactors. The authors found that IFSB "demonstrates the capability to produce an antibody-based biotherapeutic with either comparable or enhanced bioprocess performance and product quality" by reducing shear damage to cells, and enhancing viable cell density (VCD) at the 5-L scale. In addition, the authors found that protein expression was increased from 2.56g/L to 3.70g/L, with reduced acidic variants from 27.0% to 21.5%. A high VCD was also experienced for both 50-L and 500-L scale-ups . Paper 03: 112 words, Time: 8 minutes 7.