The modified geometrical route with the Boresch restraints was employed to calculate the binding free energies of the coiled coils depicted in Figure . When we refer to binding free energies ∆G • bind hereafter, we mean aggregation free energies of fully folded α-helices. Coiled coils of different oligomerisation state are then compared with the metric ∆G • bind per helix (divided by the number of helices in the complex). Details about the metadynamics protocol with Boresch restraints are provided in the SI (SI Section 5).