For sample preparation, pipette or Echo liquid handling robots diluted and mixed up to ten compounds in a well with a concentration of between 5 µM and 20 µM. We developed a dualpump flow-injection MS n method to gather high-quality spectra on multiple adducts of up to ten compounds in a single injection. The acquisition of an ion's MS n tree is time-consuming and can easily span multiple seconds, depending on the tree's depth and breadth. Additionally, the mass resolution and maximum injection time, together with the automatic gain control (AGC) setting, limit the scan rate to 5-8 Hz. For example, the presented method resulted in a maximum acquisition time of ~ 13 seconds per picked MS 2 precursor ion with up to MS 5 , 25 precursor ions (5 for MS , 5×2 for MS , 10×1 MS 5 ), 75 spectra including three collision energies, and a 200 ms maximum injection time. Consequently, this renders fast LC-MS unviable, considering its compound elution profiles in the lower seconds range. We optimized a high-throughput flow injection method to reach a rectangular-current intensity profile of 1.5 minutes within 3 minutes of total analysis time, including a washout phase (see Fig. ). The final method provided enough time for ten mixed compounds per injection, including multiple adducts, considering that only a few precursors trigger a full MS n tree. This method is based on a dual-pump liquid chromatography system with a sample delivery pump and a secondary makeup pump to modulate the elution profile and improve washout behavior (see the Online Methods). Most crucially, the data-dependent acquisition (DDA) mode with combined static and dynamic precursor exclusion was used. The static exclusion list was generated for measured blanks (i.e. the dilution solvents) in each polarity. After that, mzmine was used to extract all m/z values with a minimum intensity ten times lower than the intensity threshold set for the Orbitrap ID-X to trigger MS 2 and those detected in multiple blank injections. The dynamic exclusion was set to 1.2 min-width to guarantee better coverage of various ion adducts and compounds within the same run. This still allowed individual ions to be picked at the start and end of the elution peak.