Plate panning was performed regarding the general concept presented by Barbas After washing, 100 μl of elution buffer (0.2 M glycine-HCl, pH 2.2) was added, and after 10 minutes it was collected. 10 μl was used to titrate the output phase, while 90 μl was directly transferred into the bacteria culture for amplification. Lysates (products of amplification and purification of phages) were tittered and used for subsequent pannings or single clone isolation.