In the sorgoleone synthesis pathway (Fig. ), a specialized polyketide synthase called acyl-resorcinol synthase forms the aromatic ring and links this ring to the C-16 acyl tail which results into a compound called 5-pentadecatrienyl resorcinol. Then 5-pentadecatrienyl resorcinol with the help of an O-Methyltransferase is converted into 5-Pentadecatrienyl resorcinol-3-methyl ether, which is then converted to dihydrosorgoleone through a putative P450 monooxygenase (Fig. ). Acyl-resorcinol synthase enzymes are intracellular, and are localized to the membrane surface. O-Methyltransferase and a putative P450 monooxygenase are localized to the cytosol, but were not tested for membrane binding. Localization of sorgoleone synthesis pathway enzymes to the cytosol suggests that sorgoleone is manufactured on the plasma membrane or the endoplasmic reticulum, even if localization of O-Methyltransferase and the putative P450 monooxygenase are not well established.