To study if C1 can inhibit the cellular biogenesis of miR-377, its effect was studied in HUVECs, as they differentiate into tubules in a miR-377-and VEGFA-dependent manner, which can be used to measure angiogenic capacity directly. 27 C1 reduced mature miR-377 levels in a dose-dependent fashion, with an IC50 of ~5 μM (Fig. ).