The new combined workflow utilizing MP and Byos was able to annotate all peaks that were proposed in the original publication (Figure ). We observed the full linker-payload (A, A1) as well as partial loss (B, B1, C, C1, D, E). Proposed structures B, B1 were loss of 2 methoxy groups at the terminal of PBD ring. Proposed structures C, C1 were loss of PBD payload by cleaving between benzene and ester group. Proposed structure D and E were further cleavage along the linker amide bond. All proposed structures based on intact mass would require additional experiments such as middle-down characterization using collision-induced dissociation (CID) or electron activated dissociation (EAD) to fully confirm identity. To compare quantification between new and previous workflow, we plotted the change in major metabolites in LC as percent fractional abundance, which was calculated from peak intensity over total peak intensity at each time point for each animal (Figure ). The new data analysis workflow successfully recapitulated the overall trend in previous publication by Huang et al. The minor percent difference between new and previous values (Figure ) could be attributed to difference in deconvolution algorithm and the parameters used in BioPharma Finder vs Byos software.