In this assay, test substances were diluted to 100,50,25 and 12.5 μg/ml concentrations in a 96well microtiter plate. Each well contained a 0.01% resazurin dye, test metallogel, and bacterial suspensions. Ciprofloxacin (10 μg/ml) served as the positive control, while bacterial cultures in DMSO without any test metallogel act as the negative control. Three replicate plates were prepared and incubated at 37°C for 8-10 hours. The minimum inhibitory concentration (MIC) was determined as the lowest concentration that retained the blue colour of resazurin, indicating no bacterial growth .