Most recently, we have developed fluorescent antagonists, which are capable of detecting GLP1R in its unstimulated/antagonized state at the membrane . Advantageously, these probes, termed LUXendins, are equipotent to native antagonist, work well in the periphery and brain, display excellent brightness and can be formalin-fixed . To date, LUXendins have been freely and widely distributed to dozens of other labs for academic use , opening up new GLP1R biology. The LUXendins were necessarily furnished with red and far-red fluorophores, not only allowing conventional microscopy, but also for the aims of our study, total internal reflection (TIRF) microscopy and stimulated emission depletion (STED) nanoscopy . Aiming for more experimental modalities and taking on board comments from end users, we now expand the color-palette of the LUXendins, further increasing their utility for widefield, confocal, intravital and near-infrared microscopy, allowing imaging from the single cell to the whole animal.