These results highlight the potential of CuO NPs to stimulate field research and help reduce the use of conventional fertilizers and promote more sustainable agricultural management. However, long-term studies and trials under real field conditions are needed to assess the viability of this technology in agricultural systems. It is also essential to study the interaction of CuO NPs with conventional fertilizers and to carry out ecotoxicological studies to ensure the environmental safety of their application. In this sense, we suggest that future work should investigate the optimization of CuO NPs concentrations for different soil types and crops, as well as the assessment of long-term ecological effects, in order to better understand the applicability of NPs in the field.