As a first step, PDMS 3D microscaffolds were fabricated via 2PLP (see Figure in the Suppor�ng Informa�on), which offers several advantages compared to the conven�onal micro-replica molding methods. 2PLP enables not only the construc�on of mul�ple 3D scaffolds with varied geometries in a single print, but also avoids rough surfaces and ar�facts which are typically observed when using molding methods, causing undesirable topological defects . For this purpose, a commercially available PDMS-based ink (IP-PDMS, Nanoscribe GmbH) was employed . Due to the so� elastomeric proper�es of PDMS, a specific prin�ng protocol was developed involving first prin�ng a densely crosslinked contour at a slower scanning speed (20 mm/s, 30 mW) and then rapidly filling the interior (100 mm/s, 40 mW) to ensure good structural stability.