The 2'/3'-formyldipeptidyl-esters are equivalent to the acceptor stem of a tRNA at the A-site after peptide bond formation but before translocation. In extant translation, releasing the mature peptides is assisted by the Gly-Gly-Gln motif in the Class I release factors. But hydrolysis of 2'/3'-peptidyl-esters in a prebiotic context has not been documented to the best of our knowledge. In this work, with the help of aminoacyl transfer chemistry, we have built a small but diverse series of 2'/3'-(N-formyl)dipeptidyl-esters and characterized their hydrolytic stability (Table ). The longest half-life among the six 2'/3'-formyldipeptidyl-esters is 2'/3'-fAla-Leu-ester (t½ = 6.6 days at 10°C, pH 6.8). This suggests that peptidyl RNA conjugates can survive and become enriched in the prebiotic environment without auxiliary macromolecules.