We performed the experiments shown in Figure for the timing-controlled division of the A:B-droplets. The droplet division reaction started by adding active triggers (TABi), inhibited triggers (iT † ABi), excess inhibitors (R † ABi), and RNase H into an A:B-droplet solution (Methods in detail). Figures and show time-lapse images of A:B-droplet division. The required time for the division was elongated with decreasing 𝑐 ' "# or increasing 𝑢 ) & $%'