In Figure we provide a summary of all APL and Dʜʜ thickness measurements from extended simulations of lamellar bilayers (512 lipids each, 1 µs of production simulation) using the Martini 3.0.0 4 lipid models, together with the parameters obtained for Representations 1 and 2, along with reference experimental data . We obtain improved parameters of both Representations 1 and 2 with respect to Martini PC lipids version 3.0.0 in the context of this training set, demonstrating the usefulness of such optimization protocols even when exploiting only the APL and Dʜʜ thickness measurements during training. For Representation 1, however, the transition in between liquid and gel phases is not observed for DMPC and the transition temperature is not respected for DSPC (Fig. ). This can be associated to the usage of a mixture of small and regular beads in the lipid tails (Fig. ). For Representation 2 the transition temperatures are better respected, while also the behavior of lipid mixtures appear improved, as a posterior result of the optimization of the structural properties in pure composition bilayer simulations.