Cells were counted using Biorad TC20 Automated Cell Counter combined with a trypan blue exclusion assay to determine viability. Number of cells mentioned hereinafter correspond to live cells counted otherwise stated. U-87 MG (ATCC HTB-14), KB (ATCC CRL-3596) cells were grown in Eagle's Minimum essential medium (EMEM, Gibco Invitrogen), supplemented with 10% fetal bovine serum (FBS, Lonza), 2 mM L-glutamine (Gibco-Invitrogen), 1% non-essential amino acid solution (Gibco-Invitrogen), sodium pyruvate 1 mmol/L and phenol red at 37 °C in a humidified 5% CO2 atmosphere. HEK293T (ATCC CRL-3216), LNZ308 (CVCL_0394), EA.hy926 (ATCC CRL-2922) cells were grown in Dulbecco's Modified Eagle's Medium (DMEM) with supplemented with 10% fetal bovine serum (FBS, Lonza), L-Glutamine and phenol red at 37 °C in a humidified 5% CO2 atmosphere. Cells were seeded onto a chambered coverglass (IBIDI) at a density of 5 × 10 4 cells/well 24 h before the microscopy measurement. For microscopy imaging, the attached live cells in IBIDI dishes were washed once with warm OptiMEM solution (OptiMEM , Gibco-Invitrogen); after that, 1 mL of a corresponding dye solution in HBSS was added and the cells were incubated for 5 min at room temperature. Then, the attached cells in IBIDI dishes were washed twice with HBSS before imaging.