We use extensive all-atom molecular dynamics simulations to explore the microscopic characteristics of water in the presence of co-solvents. In this investigation, we use reline as the co-solvent in a solution which consists of a well-known protein denaturant urea and a well-known protein secondary structure protecting osmolyte choline chloride. We gradually increase the amount of reline within the system to observe the change it could invoke around the water. An accumulation of water molecules is observed while the wt% of reline is increased in the system, as evident from the radial distribution plot. There is also the likelihood of more amounts of co-solvents surrounding the water molecules (Fig ). The position of the water-oxygen (Ow) self pair-correlation peak is in good agreement with that of the experimentally verified one . Further insights are drawn from the relative number fraction ( 𝛷 𝑖 (𝑟)), which accounts for the spatial occupancy of the constituents surrounding the water-oxygen (Ow). It shows whenever there is an increase in the amount of water surrounding the water molecule there is an overall decrease in the amount of urea and choline (Fig ).