A 96-well plate coated with an antibody specific to Human PSA was used. A standard PSA solution of 80,000 pg/ml was diluted in Sample Diluent NS to perform calibration curve measurements of 62. Venous blood was extracted and centrifuged to coagulate and remove the red blood cells. The test was performed directly on the separated plasma fluid remaining after diluting by a 4-fold in Sample Diluent NS provided in the kit.