To understand many of the biological pathways that could be affected by the complex mixture of plastic-associated chemicals, the solvent extracts from the spilled plastic were screened for their bioactivity using several FACTORIAL bioassays (TF, NR, and AquaTox). In total, across the three different bioassays, the activity of 70 human transcription factor response elements and nuclear receptors and 6-7 nuclear receptors for each of three phylogenetically distinct fish species were measured in response to the solvent extracts from white nurdles, burnt plastic, and combustion remnant pieces.