50°C for 10 min 1 Enzyme activation 95°C for 3 min 1 PCR Amplification 95°C for 1 sec 2 60°C for 20 sec 95°C for 1 sec 40 60°C for 5 sec Table . The temperature conditions for each step of the RT-qPCR. Although the optimum temperature for the enzymes is 72°C, the cycle does not change the temperature for simplicity and faster speed in PCR testing.