Then, vacuum filtrated device was used to filter the products solution. During the process of filtration, filter membrane with 0.2 µm holes was used. After the filtration, the extracts were washed with water three times by vacuum filtrated device. The final extracts were obtained after dried with vacuum oven for 4 h. Environmental microplastics samples from Warnow River and Baltic Sea were extracted using an enzymatic and chemical purification protocol. Parts of the extracts were filtered using Si filters (10 µm holes). The residues onto the Si filter were analysed by Raman microscopy. Subsequently they were dispensed with MilliQ-water for SEM investigations and removal experiments.