Levine et al. also studied the effect of the concentration of T4 bacteriophage DNA on the degree of its denaturation. The authors show that a higher degree of DNA denatura-tion in 1.0 M urea at 346 • K occurred in more dilute solutions for DNA. This observation fits the prediction based on analysis of Eq. 30. An increase in DNA concentration, V f , in the second term on the right-hand side of Eq 30, leads to a decrease in the lnK term.