It was remarkable that targeting central, lipid or elongated 1k4c cavities, only one dominant scaffold was predicted for each of those cavities (representative top-child scaffold) (Figure , Table ). It was also remarkable that despite the many peripheral atom variations for each dominant scaffold, most of the 100 top-children targeted similar docking cavities than their corresponding parents (Supplementary Material /*.pse). Some critical interactions between the top non-toxic fitted-children representatives and the amino acids at the KcsA chains included previously described amino acid contact in several chains. For example, the 64 R and 89 R implicated before in lipid head interactions (Table , red numbers), the 75 T of the K + binding 75 TVGYG sequence (Table , green numbers) and/or the inner α-helix 100 I and 103 F residues known to stabilize qac binding (Table , blue numbers). There were also other amino acids implicated in the elongated cavity that were not targeted by the other ligands. Nevertheless, despite having longer alkyl-chains, most of the elongated non-toxic fittedgrandchildren predicted 10-100-fold lower affinities than those evolved by targeting the central or the lipid cavities (Compare affinity values of Figure , green star) data with the non-toxic fitted-children candidates described here predict that none of these research on-going molecules predicted higher affinities than most of the children candidate alternatives proposed here. and Supplementary Material Red spheres, Oxygens. Blue spheres, Nitrogens. Light green spheres and sticks, Carbons and bonds. 7810, top-child predicting docking to the central cavity (Figure ). 34511 top-child predicting docking to the lipid cavity (Figure ). 20346 top-grandchild predicting docking to the elongated cavity (Figure ).