In the present work, we investigated electrochemical and spectroscopic properties of 8aminoqinoline (8AQ). The conducted research was supported by quantum chemical calculations. On the basis of spectrophotometric titration, it was noted that 8AQ exists in two formsmonoprotonated (8AQH + ) and neutral (8AQ) which is closely related to the pH of the solution. Recorded voltammograms in solution from pH 3 up to 10, in anodic potentials, exhibited two oxidation peaks. The first one (Eox1) was located as a shoulder of the second oxidation peak (Eox2). Based on the linear dependence of the peak currents vs. the square root of the scan rate second oxidation (Eox2) process can be described as diffusion-controlled. In contrast, the first peak (Eox1) is associated with the adsorption of the substrate on the surface of the GC electrode. We have shown that the protonation of 8AQ affects the oxidation pathway of this molecule, which was further elaborated using high-level quantum chemical calculations. Experiments carried out in the cathodic potential range revealed that 8AQ undergoes a reduction reaction at very negative potential values below -1.2 V (vs. Ag/AgCl). The obtained linear relationship Ered vs. pH with slope of 59 mV per pH unit indicated that the reduction process over the entire pH range follows the same mechanism and involves the same number of electrons and protons.