In vivo fluorescent imaging (Fig. ) showed that the fluorescence signal was primarily concentrated in the cotyledons, with a central intensity peak that diminished towards the periphery. The intensity of the collected fluorescence of TBP (reaction products of TBPBB and ROS, part S6 in SI) showed a gradually increasing trend (Fig. ), indicated a positive correlation with increasing concentrations of stress agents (ROS) during the germination. Fluorescent images of pea sprouts coming from the germination process from 36 h to 168 h and then incubated with TBPBB for 1 h in dark, (G) the adsorbed TBPBB concentration determined from UV-vis spectra, (H) The fluorescence intensity of TBP of the peas captured from (F),  ex: