In developing this new platform our philosophy was to not only achieve depth of proteome coverage for each single cell and demonstrate that we can specifically target the low abundance part of the proteome, but to also show the practicality and applicability of this innovative workflow. For example, we showed that we can monitor cell heterogeneity under different biological conditions and study particular pathways. The platform also lays the foundation for rigorously investigating protein posttranslational modifications (PTMs) at single cell resolution. We purposefully built SCREEN to allow proteomics laboratories around the world to reproduce what we have done and perform their own high quality single cell analysis. To this end, all required reagents and consumables for this platform are commercially available and don't require specialized solutions such as ultra-precise microfluidics, hard to achieve chromatographic performance from nano columns, and ultra-low flow rate liquid chromatography setup which make the analysis time impractical for large cell population studies. All parts of the platform have been tested successfully for reproducibility and the platform has been applied to a variety of cell types under different biological and experimental setups. We have also benchmarked the platform. The current throughput is 700 cells per week utilizing a single mass spectrometer equipped with a tandem nano HPLC pump, fast trapping column injections and a double column set up for TMT11plex method. Under this configuration 8 single cells can be quantified per mass spec run. Utilizing a TMT16plex based scheme performing 12 single cells per mass spec run, we can analyze at least an unprecedented 1000 cells per week.