Although several parameters need to be chosen a priori as stated in Step 1, simulation time τ can be selected without having to worry about fulfilling the Markovian property, a requirement that other enhanced sampling methods have such as Markov State Models (MSMs) and milestoning. The simulation time τ should be chosen to be short enough so that WE does not inadvertently miss transitions. However, since many macrostates have to reach convergence to extract correct thermodynamic and kinetic properties of the system, WE can be computationally costly if the initial states are not close to steady-state.