A diverse range of AAs and metabolites were observed and quantified across the 13-month sampling period (appendix 1). In total, 17 parent AAs and 8 metabolites were quantified consistently in wastewater in both sites during the sampling period. AAs and metabolites from the macrolide and sulphonamide classes were well represented, with AAs from the aminoglycoside not detected once during the sampling period. Average wastewater concentrations for the consistently quantified AAs and metabolites were reported at 0.50 ± 0.58 ng/L for Bath and 0.52 ± 0.75 ng/L for Keynsham over all studied months. Whilst the concentration of AAs and respective metabolites in wastewater will be impacted by consumption patterns at the community level, variable wastewater flowrates will also have an impact, highlighting the importance of normalising concentrations with flowrates when monitoring both spatial and temporal trends.