Mice were randomly assigned to the experimental groups of four males. 5m and the sham-treated group were administered intraperitoneally (i.p., 10% kolliphor in saline with 5% DMSO). Based on described by Gorecki et al. , in methanol) for brain homogenate, 5 µl for plasmatic samples, with the final concentration being 100 nM, then the sample was vortexed and 100 µl of 1 M sodium hydroxide (brain homogenate) or 50 µl (plasma) and 700 µl of ethylacetate (ACS reagent, VWR chemicals, Fontenay-sous-Bois, France) for brain homogenate or 350 µl for plasma was added. The units, auxiliary gas: 12.5 arbitrary units, spare gas: 2.5 arbitrary units, probe heater temperature: 300 °C, max spray current: 100 µA, S-lens RF level: 50.