Our investigation into vitamin D's anti-aging potential started with evaluating its binding affinity with several key aging proteins. To elucidate how vitamin D modulates aging effects, we selected six aging receptors known as molecular targets in aging research. Three distinct docking simulations were conducted: docking with a grid, blind docking (without a grid), and hydrated docking. Both vitamin D2 and vitamin D3 were docked to each protein, with ten independent docking calculations performed for each.