Beyond assay reagents, efforts to engineer our AmpliFold approach -comprising sequential capture, cleavage, and re-binding operations -within a single, comprehensive paper microfluidic framework necessitated the ability to perform multi-step LFA processes with controlled liquid flow. For the purposes of demonstrating our 'capture-and-release' approach as a proof-of-concept, a rudimentary 'two-strip' LFA procedure was employed where the capture (PSA-printed) strip was manually interfaced with the detection (anti-FITC pAb printed) strip using a ribbon of adhesive tape to maintain membrane-tomembrane contact. In essence, this assembly would be performed following the capture of material and wash steps, prior to the introduction of a thiol-based cleavage buffer. A full step-by-step schematic outlining a typical AmpliFold workflow is shown in the Supplementary Information (Figure ). This procedure enabled us to explore the capabilities of our AmpliFold approach within a proof-of-concept design which, at this stage, necessitated additional user steps, such as the manual removal of the capture strip absorbent pad and the transfer of strips 'well-to-well'. Ultimately, it is envisioned that future iterations of an AmpliFold device could simplify the manual assembly of capture surface by anti-fluorescein antibodies. This binding process is different from that of the capture step as our FluoroPerAuNP conjugates demonstrate high degrees of fluorescein presentation across the entire particle surface, vastly improving the rate of successful collisions at the anti-FITC test line (Figure ). As a result, the re-binding of immunocomplexes during the detection step occurs with faster association kinetics and higher affinity, thus yielding sharper test line profiles and amplified signal-to-noise compared to initial capture stages.