After preparing both structures, AutoDock Tools generates grid and docking parameter files. Following that, AutoDock performs the docking technique, forecasting the optimum ligand binding locations inside the protein's active site and providing information on likely binding affinities and interactions. The alpha hemolysis binds to the plasma membrane and results in pore formation on the surface of the plasma membrane, which results in cell lysis. We hypothesize that blocking the pore of the protein could block the pore formation on the cell surface and prevent cell lysis. We have docked chemical ligands that could bind in the protein's pore. We have identified five ligands that can bind to the protein pore and potentially inhibit it. In the current investigation, Ligands III and IV form the strongest interaction with the protein pore.