The intramolecular restraints are required to prevent distortion of the intramolecular degrees of freedom by the intermolecular restraints, which would otherwise introduce error into the ∆G o Release calculated using Equation . The strength of the intramolecular restraints required to eliminate this error is dependent on how how permissive the intermolecular restraints are; highly restrictive intermolecular restraints necessitate aggressive rigidification.