Fifty-three of the FBMN matches were also annotated with NAP. Thirty-seven of these annotations result from at least one structure candidate of the in silico fragmentation search with the MetFrag algorithm , including 25 unique spectra. NAP allowed the annotation of 156 not previously annotated features, using only in silico fragmentation search with Met-Frag. Hence, the in silico fragmentation search allowed the annotation of 39.8% of all the considered features by the NAP algorithm versus 4.2% of all data set using only experimental spectral database search. In total, NAP led to the annotation of 9.9% of all the detected features.