We extracted lyophilized carrot powder with four different organic solvents, spiked the extract with our test alkyne 1, and subjected these extracts to RuAAC using three different azides (2a -2c). Our procedure entailed adding of small amounts (1 mg) of solid catalyst powder directly to reaction vials, an operation prone to imprecision in the exact amount of catalyst added. Thus, to fairly compare the reactivity of different azides, we used two azides in each reaction, ensuring a given azide pair could be compared at a fixed catalyst loading.