Ultimately, twenty-three compounds were successfully measured for pH-solubility using an HTE approach. These included weak acids, weak bases, amphoteric, and (apparently) non-ionizable molecules. The primary goal was to efficiently conduct the experiments with a simplified and standardized design, while also ensuring accurate data capture for the range of Britton-Robinson buffers selected. A primary limitation to consider was a lack of abundant drug substance availability, so it was determined that experiments which utilized a 96-well plate were ideal for this first portion of the study. The limitation of available drug substance also prevented any possibility of running these experiments with replicate samples. Following sample preparation, the vials were mixed for 24 hours at room temperature to ensure that full drug saturation was achieved. A standardized analytical HPLC method was developed with the intent of using the same primary method for all drug substances investigated in this study, with the exception of establishing the appropriate wavelength and retention time for each drug substance. The final pH of each sample was collected from the multi-tip pH probe configuration on the Unchained Labs CM3 platform. This automated pH measurement process includes a water bath followed by blow-drying each pH probe in between measurements. It is possible that some error is introduced into the final pH reading, if there remains a small droplet of water on the pH probe when it is being inserted into the 500 µL volume sample. This is likely not to be a considerable introduction of possible error, but it needs to be included as a possible source if one exists. This experimental approach seemed ideally suited for pKa determination. With solubility data that was collected, ionization constants were computed using Kaleidagraph software . Once established, the ionization constants were then used to confirm at which pH the appropriate aqueous buffer would be selected for the subsequent distribution coefficient experiments.