At last, we examined the effect of the sample temperature on the critical dose tolerance limit of ZIF-8. The sample is cooled down to cryogenic temperature, and the critical dose of {431} and {110} Bragg planes are measured at 200 kV and 300 kV, as shown in fig. . Fig. ) shows the diffraction patterns of ZIF-8 cooled down to cryo temperature at 300 and 200 kV, respectively. Fig. ) shows the fading of the relative intensity of {431} and {110} Bragg plane at 300 kV and 200 kV, respectively, under cryo temperature. The critical dose of {431} and {110} Bragg planes at 200 kV were determined as 10.7 ± 2.5 e -Å -2 and 19.7 ± 3.8 e -Å -2 , respectively, which increased at 300 kV to 17.7 ± 2.6 e -Å -2 and 57.0 ± 9.2 e -Å -2 , respectively. Note: The local peak maxima in the relative intensities of Bragg planes are results of particle reorientation during the exposure of an electron beam at room temperature (RT) as well as at cryotemperature . We also investigated if there is an effect of any potential presence of a residual volatile substance in the pores of ZIF-8, e.g., water/moisture or other solvents, which might cause additional interaction with the electron beam. ZIF-8 was heated in the TEM to 125°C for 1 hour using an in-situ heating holder for sample drying and then cooled back to RT. Fig. ) shows diffraction patterns of ZIF-8 at 300 kV at 125°C and after cooling down to RT, respectively. Fig. ) shows the fading of the relative intensity of {431} and {110} Bragg plane at 125°C and RT. The critical dose of {431} and {110} Bragg plane at 125°C was measured as 7.3 ± 1.2 e -Å -2 and 26.0 ± 0.8 e -Å -2 , respectively, and plotted in fig. ). After cooling down to RT, the critical dose for {431} and {110} Bragg plane was again around 10.0 ± 0.8 e -Å -2 and 34.0 ± 2.2 e -Å -2 , respectively, close to the values at RT measurements (fig. ).