With four key model parameters already calibrated using the batch experiment data, the remaining four parameters were calibrated using 25 days of reconciled data from March 12, 2022 to April 5, 2022. The model aimed to predict microalgae biomass concentration (g-VSS•m -3 ) in the return tank, effluent orthophosphate concentration (g-P•m -3 ), and biomass yield on the limiting nutrient (g-biomass produced•g-P removed -1 ; this value is particularly important for solids management and the finances of the treatment plant). Biomass yield was calculated as a 1-day moving average to be at a similar timescale as the solids residence time (SRT) of the EcoRecover process (3-5 days). Using the identical TPE algorithm, the remaining four parameters (to which model outputs were most sensitive) were calibrated to the following values: exp (7.57), f CH max (9.61 g-COD•g-COD -1 •d -1 ), arre (6663.36 K), and KP (6.07 g-P•m -3 ). During the parameter optimization process, certain combinations of parameter values required an excessive amount of time (~hours)