Another less common strategy for some specific microorganisms is cyclopropanation of the lipid acyl chains that protect the membrane from adverse effects of freezing during drastic cooling . Lipids with cyclopropane ring containing fatty acids (LCFA) are widely distributed in a variety of organisms, particularly in bacteria, e.g., Escherichia coli, Lactobacillus, Rhizobium, etc. They are generated in situ via methylation of the cisunsaturated fatty acid double bond in presence of cyclopropane fatty acid synthase and the concentration of LCFAs rises as the cell is exposed to a hostile condition . LCFAs are known to disrupt the ordering of the lipid acyl chains and thereby keep the membrane fluid at lower temperatures . Similar effect of LUFA is already known . Henceforth, the role of cyclopropane ring is akin with that of the double bond, as evident from the earlier literature survey.