In addition, the small molecules exhibit optimal molecular size and lipophilicity conditions that maximize clinical potential, making them promising agents against antibiotic-resistant Salmonella. Because many T3SS-dependent bacteria have similar secretion systems, the compounds identified here for Salmonella ATPase inhibition are favorable for broad-spectrum therapeutic development. The central T3SS ATPase among gram-negative bacteria is highly conserved, generating potential for T3SS inhibitors to treat a variety of infections.