Here, we present an extended HDX-MS system that enables automated sample delipidation for lipidsolubilized membrane proteins. We incorporated a ZrO 2 -packed phospholipid trap column online with protein digestion and subsequent peptide analysis. This setup allows phospholipids to be retained in the ZrO 2 matrix of the phospholipid trap column, while proteins pass through and undergo digestion with subsequent peptide trapping. Therefore, the system not only provides an automated but also an economic, and environmentally friendly way of protein delipidation (i.e. reduction of phospholipid trapping beads and plastic usage). We compared ZrO 2 and TiO 2 beads in terms of delipidation efficiency and unspecific protein/peptide binding. ZrO 2 beads have been shown to outperform TiO 2 with delipidation efficiency, which was well above 99% for POPC throughout a course of 30 injections. The efficiency of ZrO 2 beads was also shown to be independent of the type and length of phospholipids, and unlike TiO 2 , even capable of retaining Fos-choline-12. We also assessed the level of back-exchange and peptide carry-over for the extended system, verifying that both do not show any noticeable increase compared to the standard HDX -MS configuration.