In terms of chromatographic performance, it appears that the different capsid fill states of AAV5 are best separated, followed by AAV6 and AAV8. AAV8 moreover shows an increased peak width when compared to serotypes 5 and 6. Calculated chromatographic resolu9on for each separa9on supports these findings (Table ). With an experimentally determined chromatographic resolu9on of 4.30, AAV5