First, we measured free MSP1E3D1 -the utilized MSP protein -on the standard two-valve HDX-MS configuration. Subsequently, we applied the extended delipidation configuration to measure MSP1E3D1 in the free as well as nanodisc state (Figure ). The base peak ion chromatogram displays 2-3 x lower intensities when performed on the extended three-valve configuration delipidation system (Figure ), which is in agreement with previous observations for unspecific protein adsorption under optimized conditions (Figure ). More importantly, intensities obtained from analysis on the delipidation system both in absence and presence of lipids, i.e. free and nanodisc MSP1E3D1, are of equal magnitude.