Classification System (BCS) classes has been chosen for co-permeation experiments (Table ). Based on their lipophilic/hydrophilic character, the drugs were incorporated into liposomes either by the aqueous route (i.e., dissolved in the hydration medium together with CF) or by the lipidic route (i.e., dissolved in chloroform and methanol together with the membrane lipids). For lipophilic compounds mildly solubile in water (HCTZ and NX), both loading methods were used (Table ). The acronyms of individual substances are given in Table . The data points are mean values, the error bars represent standard deviations (n = 3). Note the difference in the y-axis scale between cases (A) and (B).