Protein-liposomes sample preparation for Cryo-TEM. To prepare protein-liposome sample, liposomes (2 mM, 70:30 DOPC: Cholesterol) were incubated with PLY (1 mg/ml) for 1 h, then Cymal-6 (0.56 mM) was added and 10 min further incubated at 37 ºC, after this sample was ready for application on to the Cryo-TEM grid. In case of protein-inhibitor and liposomes, samples were prepared in exact same manner except, PLY (1 mg/ml) was pre-incubated with PB-2 (100 μM) for 10 min. Liposomes (2 mM) alone sample in PBS was kept as control (n =