where V (s(R, t)) is the time dependent bias used during the simulation, and the exponential is averaged over the WT-MetaD run. The main idea behind this approach is to infrequently deposit the bias onto the free energy landscape to speed-up the transition and to effectively observe it at atomistic resolution during the run. At the same time, the V (s(R, t)) has to be deposited infrequently during the WT-MetaD, in such a way to prevent/minimize the deposition of bias on the transition barrier. The transition times (t) estimated from multiple infrequent WT-MetaD runs allow building a transition probability distribution, P n 1 , defining the probability to effectively observe at least one transition by time t: