One of the matrices that could be used for analysis of this molecule is the N-(1-naphthyl) ethylenediamine dihydrochloride (NEDC). It was used for the first Wme by Chen et al. , who analysed glucose in rat brain micro dialysates using dried droplet approach. Measurements shown that peak for m/z 215.03 is characterisWc for glucose adduct with chloride in the negaWve ion mode [glucose+ 35 Cl] -. Chen's group was able to perform quanWtaWve analysis of glucose in micro dialysates using mix of glucose soluWons. Since the NEDC matrix has a significant tolerance for high salt concentraWons it was also used for mass spectrometry imaging in the negaWve ion mode. ApplicaWon of Matrix NEDC was opWmized by Guo et al. , where they used special EFASS system (electric field-assisted scanningspraying) for matrix spraying. In their research they sprayed matrices (NEDC, 9-AA, and DHB) in three different voltages on mouse Wssues, to facilitate small metabolites analysis. Glucose was only menWoned here as a possible molecule for analysis. Barre et al. used SunCollect spraying system for NEDC and 9-AA matrices for lipids and metabolites analysis. They combined in vivo imaging and MSI to track molecular signatures in diffuse large B-cell lymphoma -there was no direct glucose analysis menWoned here. Matrix NEDC was also used by Wang et al. who used MALDI MSI for colorectal cancer liver metastasis analysis. In their research they imaged glycerophospholipids and small molecules. They have shown that NEDC matrix, which was put on slide by electrospray deposiWon, is relevant for imaging glucose and glycerophospholipids in animal Wssues at a high spaWal resoluWon (10 µm) . Zahraei et al. used MALDI MSI for mapping glucose metabolites in the normal bovine lens. In their research they opWmized method for glucose analysis. Using 9-AA, DHB, 1,5-DAN and NEDC they evaluated method for sample preparaWon in bovine lens analysis. NEDC matrix was applied on the slide in two different soluWons-90% MeOH or 50%EtOH in H2O. The opWmizaWon was validated by GC-MS analysis of dissected lens regions. Due to this validaWon, they proposed spectra normalisaWon with the internal standard -to receive consistent results. That research was conWnued in the arWcle of Zahraei et al. describing ex vivo bovine lens incubaWons that uWlizes stable isotopically labelled (SIL) glucose. They used previously opWmised method for sample preparaWon: NEDC matrix in 90% EtOH, and finally, showed metabolite analysis using MALDI MSI.