To further confirm the directing effect of aryl-OH groups, analogous to CO2H and NH3 + functionalities, we prepared hydroxyaryl-H-tetrazines and demonstrated their capability for omnidirectional cleavage of C2TCO. Near-quantitative release was observed upon accelerated click reaction with rate constants of up to 1300 M -1 s -1 (Tz 16, PBS, 37 °C), which we applied to achieve efficient intracellular bioorthogonal disassembly of a pretargeted ligand-C2TCO-dye conjugate.