E. coli BL21Gold(DE3) was used to express the plasmids containing CDF-MKI4 (See Supplementary Table for more details) and pET28a-PcTS1, and was cultured in 500 mL of lysogeny broth (LB) supplemented with kanamycin (50 mg/L) and streptomycin (50 mg/L). After overnight incubation, 10 mL cultures were inoculated into 30 X 1 L flasks of fresh LB medium. When the optical density at 600 nm (OD600) reached 1.5, IPTG (0.5 mM) and isoprenol (1.0 mM) were added. The cultures were then further incubated at 28 °C and 220 rpm for an additional 18 hours. Subsequently, the cultures were centrifuged at 4000 rpm for 15 minutes, and the resulting pellet was extracted with methanol (MeOH). The organic extract was then partitioned with petroleum ether (PE).