We specified trypsin/P digestion and allowed for up to two missed cleavages for peptides with 6 to 25 amino acids. Tandem mass tags (11plex TMT) were specified as fixed modifications. Methionine oxidation (+15.99492 Da), asparagine deamidation (+0.98402 Da), protein N-terminal acetylation (+42.01056 Da) were set as variable modifications. As reduction and alkylation steps were not performed during sample preparation procedure no other modification were set. False discovery rate (FDR) calculations were performed by Proteome Discoverer search engine by Percolator module for Sequest search and Byonic hits were evaluated inside of the Byonic node independently.