(R)-1NDI and (S)-2PDI were prepared according to synthetic procedures as described in our previous literature. Coassemblies of (R)-1NDI (1.0 × 10 -5 M) and (S)-2PDI (1.5 × 10 -7 M) were prepared in a mixture of chloroform/MCH (1:19) with annealing at 95°C followed by cooling to room temperature and then drop cast onto alkyl-treated and untreated glass slides. We modified the glass slides with alkylsilanes to make the glass surface hydrophobic and compatible with the supramolecular complex. Prior to silanization, the substrates were thoroughly cleaned as follows: the glass slides were rinsed 2-3 times with acetone, then sonicated in an ultrasonic bath for 20 minutes in 50% methanol/water, followed by an additional 20 minutes in chloroform, and further allowed to air dry. The dried glass slides were treated with plasma cleaner for 60 seconds as a surface activation method. After plasma cleaning, the glass slides were kept in an oven for 1 hour at 110 °C to produce a dehydrated surface. They were then immersed in 2 vol% alkylsilane solutions (n-octyltriethoxysilane and n-dodecyltriethoxysilane) in toluene and dried in an oven at 110 °C under N2 atmosphere for 1 hour to remove toluene completely before microscopy imaging.