For data collection, the grids with milled lamellae were carefully rotated by 90° and transferred into a Titan Krios 3Gi, operating under cryogenic conditions at a 300 kV accelerating voltage. An all-grid atlas was obtained initially to locate the lamellae sites, which are characterized by the appearance of stripes of missing material with a small, semi-transparent substance in the middle. Each site was moved to the eucentric position before MicroED data collection. Data was collected from each site in a 63° wedge over 420 seconds using a Falcon 4i direct electron detector, without employing a beamstop.