Cyclic voltammetry (CV) in 1 mM FCA was carried away on the bare gold IDEs for all the six individual sensors to evaluate the repeatability in a chip. Relative standard deviations were calculated as follows: RSD% = (σ/μ) x 100, (n = 6), where σ is standard deviation and μ is mean of the limiting currents. Followed by CV were performed from -0.2 V to 0.4 V at 50 mV/s in 1 mM FCA for collector-generator (CG) electrodes. During these scans the protonator IDEs were held at 0 V while the working IDEs were swept as above. The concentration of o-PD (in mM) was evaluated in relation to the addition of 0.5 mM glucose at the surface of the modified electrode. The optimum pH activity for GOx was determined in a wide pH range (4.0 -8.0) to the addition of 2 mM glucose at 25 °C. An additional set of CV tests were conducted using 0.1 mM H2O2 and 1 mM FCA, respectively, to investigate various modifications to the electrode surfaces. Furthermore, electrochemical impedance spectroscopy (EIS) was performed after each surface modification in a solution of 1 mM FCA prepared in 10 mM PB. The charge transfer process, which takes place at the interface between the electrode and the solution, was analyzed using EIS after each electrodeposition. EIS measurements were conducted over a frequency range from 0.3 Hz to 100 kHz, at a potential of 0.2 V. Finally, SEM, EDX and AFM images were taken to visualize surface morphology among the bare Au, Pt-B, Pt-B/o-PD/β-CD and Pt-B/o-PD/β-CD/GOx modified electrode surfaces.