Multiple studies have explored metabolite adjustments to restrict or interfere with glycolysis and improve the ratio of T SCM and T CM cells. 2-deoxy-D-glucose (2-DG) suppresses glycolysis by replacing the 2-hydroxyl group with hydrogen. Utilizing 2-DG during T cell activation to limit the reliance on glycolysis pushed CD8 + T cells towards a differentiation pathway, favoring the formation of T SCM and T CM memory cells . The inhibition process preserves the formation of long-lived memory CD8 + T cells, helping to improve the efficacy and survival of CAR-T cells.