Compared to plain Amber14SB, using Amber14SB-H significantly reduced the AUE from 2.06± 0.33 pK to 0.64 ± 0.11 pK and increased the Pearson correlation from 0.42 ± 0.19 to 0.88 ± 0.05 (Figure ). Unlike previously observed for aspartate, glutamate, and lysine, we found a consensus estimate for CHARMM36m and Amber14SB-H resulted in an predictor that exceeded the performance of either method alone (i.e., AUE: 0.24±0.04 pK, Pearson correlation: 0.98±0.01).