Household dust also acts as the main reservoir of microbial taxa in the domestic environment, since it includes particulate matter from soil, plants, human and animal skin. The household dust microbiome influences the host microbiome , with some studies investigating the potential effects of dust-borne microbial taxa on allergy, asthma, immune system, and gut microbiome , , . One of the challenges of metagenomic studies of dust samples is the high diversity of indoor microorganisms , with an overall microbial diversity on Earth estimated to be approximately one trillion organisms , and between 500 and 1,000 different species reported in household dust . A study examining paired samples of dust and feces revealed significant co-occurrences of Actinobacteria, Bacilli, Clostridia and Gamma proteobacteria in both samples . Another study indicated a potential link between household dust exposure and sphingolipid metabolism impairment associated with allergic processes . Furthermore, the dust microbiome may be involved in the biotransformation of chemicals analogous to what is seen in other environments . Household dust also contains multiple endocrinedisrupting chemicals impacting human physiology, leading to triglyceride accumulation in in vitro models (pre-adipocyte mouse cells) . Alterations in metabolism due to environmental exposures, including household dust, may be partially attributed to the effect of dust modulating the host microbiome, as previously proposed . Household dust is a diverse mixture of artificial and natural particles along with biological entities such as bacteria, molds and pollen, as well as ash, soot, skin particles, and residues from building and consumer products . Consequently, the analysis of household dust is of great interest to investigate the human exposome, offering valuable insights into the individuals' environmental exposures.