Using STORM, we could perform single-molecule localization of antibodies bound to protein emulsifiers (phosvitin and apoB) present at the droplet interfaces in mayonnaise model emulsions. The binary phosvitin/SDS model emulsion showed a partial heterogeneous phosvitin distribution at the interfaces of the droplets. In our ternary model emulsions, based on SDS/phospholipids, we showed that adding a third protein emulsifier (phospholipid or apoB) resulted in heterogeneous distributions at the droplet interface. Our proposed method will enable to quantify the effect of formulation and processing on protein emulsifier distribution at droplet interfaces and ultimately on physical and chemical food emulsion stability. ). E) Extracted histogram of distances (gray bar chart), the fit over the data (blue line), and the peak analysis lines (dashed vertical lines). We calculated the relative peak amplitude of 0.36 and localization precision amplitude of 2.77, which suggests that the phosvitin distribution at the interface is partially heterogeneous. , and the peak analysis lines (dashed vertical lines). We obtained the relative peak amplitude of 0.43, which indicates a heterogeneous distribution of phosvitin in the presence of phospholipids. D-F) as in (A-C) but for an apoB/phospholipid/SDS model emulsion. We labeled the apoB with a secondary antibody conjugated to Alexa Fluor 555. We obtained a relative peak amplitude of 0.47, which suggests again a heterogeneous distribution of apoBs at the interface.