The tumor samples we studied were obtained from female and male patients aged 12 to 68 years old. Before scanning with SECM, the formalin-fixed paraffinembedded (FFPE) oral cancer tissue slices were soaked in xylene solution for 10 minutes to remove paraffin and then immersed in five concentrations of alcohol (100%, 95%, 90%, 70%, 30%) for five minutes, respectively. After rehydrating the tissue slices, they were placed in a beaker containing TRIS buffer (10 mM, pH 8) and heated to 95 degrees to restore the activity of the antigen on the tissues. 3% hydrogen peroxide was added to avoid interference from the endogenous peroxidase. Then, BSA was added to block the non-specific adsorption. Afterward, the solution of primary antibodies against EGFR or IL-6 (100 times diluted in PBS) was added and incubated for 1 h at room temperature (RT) and washed three times with Triton X-100 and PBS by placing the tissue in the buffer for 5 min. Finally, the slices were immersed in secondary antibodies -HRP (200 times diluted in PBS), incubated for 1 hour at RT, and washed with Triton X-100 and PBS as in the previous step.