NiV has developed as a fatal zoonotic disease. Bats, the natural reservoir of the virus, are adept at viral propagation and human outbreaks continue to be documented routinely. Since bats may be found worldwide, we might expect to see new epidemics in previously unaffected regions. Acute illness progression and a high death rate make a correct diagnosis challenging. The absence of accessible, affordable diagnostic tests and laboratories to process viral samples makes the situation worse. The total caseload is low, and the course of infection is rapid. Thus there is a dearth of investigations into human subjects that might yield effective therapy and prevention. The selected protein's secondary and tertiary characteristics demonstrated the protein structure-based relationships and, therefore, more comprehending properties of the protein. The protein is a fusion protein deeply associated with viral infection. Therefore, the selected protein can be a target for both protein-based drug and vaccine design against the protein to minimize viral infections.