To perform the matrix screening, the compounds mixtures were pre-dispensed to empty 1536-well white solid bottom plates with Echo 650 acoustic liquid handler (Beckman Coulter), 20nl/well each. Then, the plates were dispensed with PANC-1 cell suspension at 500 cells/5ul media density and placed in a tissue-culture incubator (5%CO2, 37ºC, 95% humidity). CellTiter-Glo ® reagent was added to wells 72 hours later for detection as described above.