Following the absorption on the cell surface, phages inject their nucleic acid into the bacterial cytoplasm to immediately express early genes and manipulate DNA replication. They are divided into two groups, temperate and virulent phages. While temperate phages can integrate their genome into the genome of bacteria (prophage), virulent phages can only replicate within the bacterium and lysate cells for the viral progeny release. Phage release from cells occurs thanks to the production of holin and endolysin which target membranes and cell walls, respectively . Bacteriophages are also able to target biofilm-embedded bacteria, by degrading extracellular matrix due to phage depolymerases, and to kill persister cells .