This study aimed to explore AA usage in two communities. AA loads were higher in Bath than in Keynsham, corresponding to the larger population, indicating that AA usage is population size driven. This observation is consistent with our previous work carried out in this region. Several of the quantifiable AAs in wastewater had higher loads in winter when compared to summer, including macrolide AAs and metabolites, aligning with increases in winter respiratory infections. Strong correlations were not observed between AAs and ARGs in Bath wastewater, which may reflect the fact that selection pressure for resistance operates over longer time-scales than the fluctuations observed in AA abundance in wastewater. Both hospital and community wastewater had AAs that exceeded PNEC values. In general, though, hospital effluents had a greater number of quantifiable AAs exceeding PNECs. Hospitals are therefore an important consideration in AMR surveillance as could be high risk areas for AMR.