The RoboChem platform successfully performed reaction optimization, resulting in conditions that produced outcomes closely aligned with the model batch reactions. Notably, a significant increase in space-time-yield, up to 565-fold, was achieved, demonstrating substantial potential for scale-up in the flow reactor. During the scale-up process, a slight improvement in yield was observed compared to the optimization carried out on the platform. This can be attributed to the fact that, for scale-up, the internal volume of the reactor was multiplied by a factor of 6, while the residence time remained the same. Consequently, an associated 6-fold increase in flow rate was necessary to maintain the desired residence time, leading to improved mass transfer facilitated by a higher Reynolds number. This phenomenon accounts for the observed increase in yield compared to the platform conditions.