Untargeted LC-MS is a practical tool to measure analytes comprehensively, however, analyte annotation/identification is a challenging endeavor, and fragmentation spectra (MS 2 ) are important for gaining structural information . One MS 2 acquisition technique is data-dependent acquisition (DDA) and it involves selecting specific parent mass-to-charge (m/z) ratios for fragmentation . Moreover, suspect screening, an intermediate approach between targeted and untargeted methods, allows to screen for analytes with known structures that may be present in the samples . Therefore, suspect screening can be combined with DDA to selectively investigate polyphenols in biological matrices.