The 20 mL scale biotransformation was performed in glass petri dish (10 cm diameter), using bromo alkene 7a (2 mM) and purified enzyme (0.2 µM) in 100 mM KPi buffer pH 7 containing 50 mM glucose, 0.5 mM NADPH and 1 mg mL -1 GDH with 15% MeOH. The reaction mixture was incubated for 3 h with shaking at 750 rpm. The reaction was quenched with 1 volume MeCN with precipitated protein removed by centrifugation (4000 rpm for 10 minutes).