[NiFeSe] H2ase is less susceptible to H2 inhibition as other H2ases and is hence an ideal candidate for application in confined architectures. However, if the local concentration was high enough it is likely that inhibition would still occur. Thus the simulation does not show the same decline in current density over time and predicts a greater amount of H2 (77.5 μmol cm -2 ). Therefore, the optimised electrolyte has effectively reached the highest current densities achievable in this electrode system due to the limitations imposed by the production of H2, and other accelerated sources of enzyme-film loss.