Figure : This graph is a t-test. A t-test shows whether there is a significant difference between the behavior of fish in different doses of caffeine. We had 3 replicates (n=3) for each dose. The confidence level used in this experiment is 0.05, meaning if the t-test value is more than 0.05, it means there is no significant difference between the different types of trials. If the t-test value is less than 0.05, it means there is a significant difference between the different types of trials.