Mouse model of M. tuberculosis: C57Bl6 female mice were housed in biocontainment racks and maintained in accordance with the Animal Welfare Act, the Guide for the Care and Use of Laboratory Animals and all applicable regulations, standards, and policies in a fully AAALAC International accredited Animal Biosafety Level (ABSL) 3 vivarium suitable for housing Mycobacterium tuberculosisinfected animals . All procedures were performed utilizing appropriate anesthetics when needed as listed in the NIAID DIR Animal Care and Use Committee approved animal study proposal LCIM-4E. Euthanasia methods were consistent with AVMA Guidelines on euthanasia and endpoint criteria listed in the NIAID DIR Animal Care and Use Committee approved animal study proposal LCIM-4E. For additional method details see SI, section 8.