There are various techniques to calculate LODs. One common technique is to use the signal-to-noise ratio and calculate at which concentration this ratio would equal to 3. However, in both LRMS and HRMS, there is not always a defined background noise level. Therefore, another technique was used which involves calculating the LOD from standard deviation of multiple technical replicates, as recommended in the Eurachem guideline and used in the LRMS workflow. Though different HRMS acquisition modes are available, such as product ion scan, data were acquired in full scan as it is the most commonly used data acquisition mode and typically one of the first steps in untargeted workflows.