To test further the correlation between experimental data and the changes in troponin dynamics due to phosphorylation we studied the cTnC G159D mutation, that is associated with familial dilated cardiomyopathy. The key effect of the mutation is that it abolishes the shift in Ca 2+ -sensitivity due to PKA phosphorylation of serines 22 and 23 that is crucial for the lusitropic effect and this may be sufficient to cause the DCM phenotype . We found that the G159D mutation, has significant effects upon the dynamics of troponin