Inorganic phosphate is one of the most abundant intracellular anions and has essential roles in a wide range of biological processes, such as energy storage and bone mineralization. Maintaining the correct levels of inorganic phosphate is crucial for the overall health and survival of organisms. At the cellular level, membrane transport proteins precisely control the transport of ions and molecules. Phosphate homeostasis is maintained by the coordinated action of type II (SLC34 family) and type III (SLC20 family) sodiumdependent phosphate cotransporters and the phosphate export protein XPR1. Malfunctioning phosphate transporters can cause a variety of diseases, including osteoporosis and nephrolithiasis, Fahr's syndrome, and Fanconi syndrome. Synthetic molecules (ionophores) embedded in lipidic membranes have the potential to treat disorders derived from malfunctioning anion transport proteins by carrying specific anions across the lipid barrier. Despite numerous studies on synthetic transmembrane transporters, including those for chloride, 12 bicarbonate 13 and sulfate, 14 to the best of our knowledge, there are no reports of inorganic phosphate transport across lipid bilayers mediated by synthetic carriers. This can be attributed to the intrinsic properties of phosphate, such as its high hydration energy (DGhyd = -473 kJ.mol -1 for H2PO4 -versus -340 kJ.mol -1 for Cl -), speciation at neutral pH (H2PO4 -and HPO4 2-, pKa 7.2), and its tetrahedral geometry, which make its binding and transport across an apolar lipid bilayer particularly challenging.