The anti-coagulant activity of solutions containing varying amounts of polysulfated mangiferin derivatives (1-3) is shown in Table . The anti-coagulant effect of the polysulfated mangiferin derivatives was examined in vitro using prothrombin (PT), activated partial thromboplastin (APTT), and thrombin time (TT) tests on human plasma. To investigate the impact of the sulfate group on the anti-coagulant activity, mangiferin sodium sulfate was also taken into consideration.