Taking the systems chemistry approach described above for the combined synthesis of GPCs and GPEs, the dry-state reaction of 10:0 lyso G>P was performed with EA and Ch, or EA and Ser. In these experiments, 10:0 lyso GPCs or 10:0 lyso GPSs were obtained in higher yields than before, alongside 10:0 lyso GPEs ( 1 P-NMR; Fig. ). The presence of each 10:0 lyso G3PX was established by 31 P-NMR, and supported by spiking studies with spectroscopic standards. LC-MS analyses confirmed the presence of both lysophospholipid isomers in the various mixtures of products.