Aqueous extraction refers to the soaking of seeds in distilled water and using this water for further analysis. 100g of N. sativa seeds were dried and stored in a 500 mL glass beaker. 150mL of distilled water was added to this and soaking was done for 24hrs. The solution was then filtered using a Whatman filter paper -1 with a pore size of 110nm. On filtration, the filtrate was sealed and wrapped in a container and store at 4 0 celsius Preparatory High-pressure liquid chromatography for extraction of Thymoquinone.