We demonstrated a fatty acid-based vesicle division mechanism driven by the chemical potential harvested from the hydration of the condensing agent EDC without relying on complex molecular machinery. Using vesicles comprising a succinic acid derivative (C10) or decanoic acid (DA), we observed that both systems, independently, could undergo division into smaller daughter vesicles even at low EDC concentrations. The DA vesicles also demonstrated self-division, supporting the robustness of this mechanism across different amphiphilic systems. This process retained encapsulated contents, suggesting potential protocell-like behavior. The findings indicate that the rate of anhydride hydrolysis and subsequent vesicle formation can be finely controlled by adjusting EDC concentration, offering valuable insights into the dynamics of primitive cellular processes. These insights contribute to the foundational understanding of developing synthetic cells capable of autonomous self-replication. The ability of DA vesicles to undergo EDC-induced division without complete disintegration has significant implications for synthetic biology. This process provides a model for designing robust, selfdividing synthetic cells that mimic early protocellular behaviors. Future research could focus on integrating genetic and metabolic components within these vesicles, advancing our understanding of the minimal requirements for cellular life. The insights gained from this study could inform the development of novel biomimetic materials and systems with applications in drug delivery, biosensing, and the creation of artificial life forms. This detailed analysis enhances our understanding of vesicle dynamics and division, opening new avenues for exploring the origins of life and developing synthetic biological systems.