Our main objective is to develop a method that quantitatively reveals the role of different intermolecular forces in DNA that are responsible for maintaining its helical structure. The controllable chemical denaturation of DNA has been selected as a tool to detect and evaluate these forces. The theory of this denaturation process and the corresponding mathematical protocol have been developed. Our approach shows that when a target molecule can be split apart by a solution in which it is placed, the forces inside the molecule can be deduced by analyzing the properties of the surrounding solution.