To investigate the loading efficiencies for hydrophilic cargo of each LLC particle, we sought to adapt a commonly used fluorescence assay involving a self-quenching dye used for liposomal systems. 5(6) -carboxyfluorescein (CBF) is quenched at high concentrations due to dimerisation and subsequent intersystem crossing. When lipid particles containing this dye are solubilised by a surfactant, the dye is released and diluted in the surrounding media, marked by a sudden increase in fluorescence intensity (demonstrated in the release profile plot in Fig. ).