1 ml of DT was added in 100 ml water. Then varying amounts of Brij S20 and Brij 58 (0.1-4 mM) was dropwise added (separately) in it with mild stirring. Brij concentrations were gradually raised to their corresponding CMC and higher to induce surfactant's self-assembly and simultaneous trapping of the DT in micelles hydrophobic core. The mixing process took place for 30 minutes in a light-restricted environment at room temperature (25 ℃) and neutral pH. Then a vacuum lyophilizer (Make: Eyela FDU 1200) was utilized to remove the excess water part. The concentration of DT in micelle was measured periodically for the next 16 days while the samples were kept at room temperature i.e. 25 ºC. As DT is heat labile compound, higher temperature values were not considered for this initial preparation and stability analysis stage. The amount of DT was measured using 4-marcaptopyridine assay method, explicitly mentioned in a previous research work . After 16 days the minimum amount of Brij that stabilized the DT (insignificant amount of degradation than day 2) was noted. That sample was again kept for 2 days at different temperature and pH (20 to 60 ℃ and 3 to 11 pH). After that DT concentration present in the micelle was again evaluated. The % concentration of DT present in the micelle were computed using the following equations .