From the FLIM images it is also possible to prepare time-gated images. From the sum of the decays of all the pixels of the FLIM image, two time gates are defined (Figure ): one capturing shorter lived emission (TG1: 0 -30 ns) and one capturing longer lived emission (TG2: 30 -120 ns). Additional time gating regions were considered based on a balance between signal distinguishment while retaining sufficient intensity (Figure ). From these defined time gates, two new time gated images are prepared.