To cure the editing plasmid in M. smegmatis after base editing, one colony was cultured in Middlebrook 7H9 broth with ADC in the absence of kanamycin. After growing to the stationary phase (4 days), the cells were plated onto a Middlebrook 7H10 agar plated supplemented with ADC. A single colony was picked and diluted into 5 mL Middlebrook 7H9. A fraction of the diluted cells were plated onto a Middlebrook 7H10 agar supplemented with ADC without kanamycin and another fraction was plated onto a Middlebrook 7H10 agar supplemented with ADC containing kanamycin. The cells whose plasmid was successfully cured could only grow on the plate without kanamycin.