Thus, overall, Cyclic IMS instrumentation with 1-pass cIM routine provided substantial improvement in peptide mapping data quality under identical HDX-MS conditions. Peptide coverage maps for SMO using SYNAPT G2-Si (orange), Cyclic 1-pass (pink), and Cyclic multipass (blue). Dashed boxes represent sequence segments not captured on SYNAPT G2-Si. Coverage and redundancy values were taken from Deuteros .