The different compounds were added to the liposomes at a concentra7on of 1 mol% with respect to the lipid concentra7on (0.4 mM) and the transport results are shown in Fig. . As assumed, aldehyde T is a good anion transporter, similar to its analogue without the aldehyde group. We did not observe any pH dependence of the anion transport by T when comparing pH 5 and pH 7 (Fig. ). Azine TT was poorly soluble in methanol or acetonitrile and preincorporated into the membrane of the liposomes during their forma7on. In agreement with our hypothesis that the two different urea binding sites would inhibit its transport, TT showed no significant anion transport ac7vity. This was also the case for smaller azine TB, which has an OH group that can interact with the phospholipid headgroups. Encouragingly, the more lipophilic azine TA did show clear anion transport ac7vity. Higher rates of transport by TA were observed upon increasing its concentra7on (Fig. ).