We validated that the truncated HIF2a molecules still induced partial charge variation by comparing the partial charge variation of the whole molecules to partial charge variation of the truncated molecules. We chose 5 HIF2a molecules at random and charged the whole and the truncated versions of the molecules. We confirmed that a subset of these molecules still produced conformer-dependent partial charge variability. The truncated molecules still had large partial charge variations of over 0.09 e at a single atom. This level of variation is nearly identical to the level of variation seen in non-truncated molecules. We also confirmed for a reference molecule that the truncated and whole molecule also produced comparable variability in calculated AHFE across 5 repeats of each (SI Figure ).