As seen in the t-test in figure , most t-test values throughout the experiment were more than the significance level of 0.05, except for the high dose caudal fin expansions and rams against the tank when compared to their behavior without an intruder. This means that there is not much significant difference between the different types of trials, meaning the Betta splendens with different caffeine doses had similar behavior despite seeing another Betta splendens fish around its territory. In addition, as seen in figure , there is no consistent pattern regarding the behaviors of the Betta splendens. For example, when the caffeine-dosed Betta splendens fish saw an intruder Betta splendens, there is no general pattern regarding whether the sampled fish will be more or less aggressive with more caffeine concentrated in their water. This indicates that across all experimental groups, caffeine consumption by the Betta splendens does not have an effect on their behavior.