in QELS module at Wyatt Technology (Waters Corporation, Dernbach, Germany). Using a wide bore flow cell allowed to accurately determine the Rh of LNPs (Figure and Table )-for LNP-1, LNP-2, LNP-3 and LNP-4 an Rh,avg= 88, 121, 111 and 124 nm and Rh,avg= 65, 80, 62, 77 nm was determined by using the wide bore and narrow bore flow cell, respectively (Table and Table ). Moreover, the determining Rh by AF4-MALLS660nm-DLS-RI enabled to establish the shape factors  for the LNPs (Table ). For all LNPs  in the rage of 0.9-1.07 was obtained, indicating that the particles slightly differ in compactness, e.g., LNP-1 with = 0.9 is more compact than LNP-2 with = 1.07.