Upon further increasing the dye content in the reaction mixture in the subsequent experiment, a decrease in the decolorization was noticed. For instance, when 5500 µL of the dye solution were added in the reaction mixture, the decolorization decreased to 70 % when an aliquot was measured after 2 min. However, as the remediation time elapsed, the decolorization efficiency increased. After 10 and 60 min, ~ 95 % of the dye was removed.