The reference trajectory was downloaded from the MolSSI website at . org/simulations/. It contains 3000 clones of the same simulation setup, with a total simulation time of roughly 1.8 ms and was saved every 0.5 ns. The sines and cosines of the backbone torsion angles was taken as the CVs and a TICA model was trained with the deeptime package. [8, 4] The PMF in the main text Figure was generated with kernel density estimation with a hyperbolic (epanechnikov) kernel and a bandwidth of 0.15.