In vivo fluorescence imaging of liver ischemia in mouse models. All procedures were approved by the Animal Care and Use Committee of the University of Tokyo. Female Jcl: ICR mice (7 weeks) were used. T-azoJSiR640 (200 M) in PBS (150 L) containing 4% ethanol was administered by intravenous injection. After probe administration, the mice were anesthetized with a combination of domitor, butorphanol and midazolam. Fluorescence images were captured at 10 min, 20 min and 30 min after administration of the probe, and then the portal vein was ligated with suture thread at 30 min. Further fluorescence images were captured at 40 min, 50 min and 60 min. All fluorescence images were captured with the CRi Maestro imaging system (CRi Inc., Woburn, MA).