Analogous experiments with CTB revealed selective and dose-dependent binding to Le x , and no measurable response for the glycocalyx models presenting Gb3 (Figure ). There was also no or minimal response to the Lac glycocalyx, which probably reflects the very low affinity of CTB for galactosides. CTB did not bind to bare SAv-on-SLB coated surfaces (Figure ), demonstrating that biotin-anchored model glycocalyces are suitable for interaction studies with CTB. CTB did bind to (Ni 2+ -NTA)3-presenting SLBs though (Figure ), most likely due to the histidine residues exposed on the surface of native CTB, illustrating that the method of anchoring the glycopolymers to the surface is an important consideration for glycocalyx model design. ), with results indicated in the table (inset). Data taken from (A) for STxB/Gb3 interactions, and from Figure for CTB/Le x interactions. Conditions: SAv-on-SLB with maximal (HA-g-Gb3)-B and (HA-g-Lex)-B coverages, corresponding to 𝑐 𝐺𝑏 3 = 0.024 M and 𝑐 Le x = 0.021 M (Table ), in HBS working buffer.