in complex with the C-nucleoside 5-methyl-2'-deoxypseudouridine (PDB: 1F8Y), which cannot undergo transglycosylation, was previously determined by Ealick and co-workers. Although that report also featured details of a complex of LlNDT with 2ʹ-deoxyadenosine, no coordinate file for this complex is available in the PDB, so a reconsideration of complex interactions is timely.