DSF experiments were performed on a Biorad CFX96 RT-PCR machine. 5 µl of SBC2 were incubated with 100 µl MN551 (2 % DMSO) or DMSO control in 50 mM Tris, pH 8.0, 50 mM NaCl and 6.6 x SYPRO Orange. The temperature was ramped up in 1˚ C steps between 25 and 95˚ C with 30 s incubation at each step. Melting curves were analysed by determining the minimum of the 8irst derivative using the Biorad CFX Manager software.