Lastly, the maximal sensitivity was observed for the 1000 V/s scan rate (highest scan rate tested) over the lower values (Figure ). This makes sense as there is an expected increase in signal over background with an increase in scan rate, in agreement with those reported previously . oxidation. The average current densities were calculated by averaging the points in the bolus region of the IT curves for each electrode and then averaging these values together between electrodes (and propagating the appropriate SEMs). a) is an overlay of the original and optimized waveforms. Point A indicates holding potential, B is the transition potential, and C is the switching potential. b) Current density was greatest for a holding potential of -0.3V over those at -0.2V and -0.4V. c) The largest signal for Trp was found to be with a transition potential of +0.6V over those of +0.5V and +0.7V. d) The average current density was highest for switching potential of +1.2V when compared to those at +1.1V and +1.3V. e) The switching potential duration of 2 ms yielded the largest average current density when compared to longer hold times (3, 4, or 5 ms). f) The scan rate that yielded the largest signal was 1000 V/s. **p <0.01, ****p<0.0001.