Additionally, a GMP compliant method that enables complete VP separation can be utilized for screening assays to rapidly confirm serotype identity before product release. However, this necessitates complete separation of AAV VPs for as many serotypes as possible. Unfortunately, chromatographic separation of AAV2 VP2 and VP1 has proven difficult to achieve using MS compatible mobile phases. While recent full-length VP separation studies have shown promise, illustrating an ability to achieve complete VP separation for multiple serotypes , those that succeed in full VP separation of AAV2 suffer from MS ion suppression effects .