The development of simplified assays for safety assessment is a key element within the changes taking place over the last decade in the field of chemical toxicology testing. By moving from the classical observation of apical endpoints in animals towards cheaper and faster assays performed on cell cultures, it is possible to cut costs and save time, paving the way to increased-throughput testing . When it comes to toxicity assessment of molecules with neurotoxic potential, astrocytes are an appealing model system, since their activation upon exposure to different neuroinflammatory triggers can take them to either neurotoxic or neurotrophic states . To check this approach, and as a first proof-of-concept, 2Dcultures of astrocytes exposed to different natural neuroinflammatory triggers, namely interleukin 1β (IL-1β), tumoral necrosis factor α (TNFα), and lipopolysaccharide (LPS). In order to study polar metabolites involved in these processes, and as a showcase of the full ROMANCE workflow on actual metabolomics data, we have analyzed the astrocyte samples using the previously described CE-MS approach. First, raw Agilent .d files were transformed to the open format mzML. Then the files were either directly imported into the Progenesis QI peak-picking software, or transformed (and area-corrected) into the effective mobility scale by ROMANCE prior to the peak-picking step. Peaks were manually reviewed to ensure correct identifications against an in-house library, producing a set of 38 identified features in ESI+ mode and 28 in ESI-mode, common to both electropherogram and mobilogram peak extraction. To compensate sample amount variability in the samples, probabilistic quotient normalization (PQN) was applied to both datasets, a widely used normalization technique in metabolomics . Drift and other analytical effects were in turn corrected with the inclusion of quality control (QC) samples to ensure analytical consistency, used to apply a principal component based correction to cancel out the sources of variability between the QCs .