Comparison of kinetic parameters of HheG and HheG-682 for the dehalogenation of 2chlorocyclohexanol (20). Reactions were carried out in a total volume of 1 mL with 1-150 mM haloalcohol in the presence of 2% DMSO in 25 mM Tris•SO4 buffer, pH 7.0, at 30 °C using 150 µg mL -1 HheG-682. Samples were taken after 30, 60, 90, 120, 150, 180 s as duplicates. Chemical background of negative control reactions without enzyme addition was subtracted. The Hill equation was used for data fitting in OriginPro. Kinetic parameters for HheG were taken from Koopmeiners et al. Enzyme K50