As the target compound (DT) is heat labile a fixed temperature (25 ℃) was considered and effect of higher temperature at this initial assessment was not done. The concentration of H + in the solution and between the micelles rises when the pH of the system is lowered. Due to the negative charges on the Brij micelle's exterior (Zeta potential: -21.22 and -26.45) the existence of H + ions cause an ionic firmness to occur on the micelle's external perimeter. As a result, the Van der Waals attracting forces increased in significance and caused the micelles to become unstable. However, a good stabilization and durability was seen in the presence of alkali because of the repulsive force caused by the same charge surrounding the negative-charged micelle surfaces . It can be explained from Figure that DT is stable in the Brij micelle that contains above CMC concentration for a duration of 16 days. In contrast, it was observed that within 24 to 48 hours, both the 'not entrapped DT' and the 'DT in the Brij micelle with sub-CMC concentration' turned unsustainable and degraded rapidly. As per figure , it was noticed that after 16 days, Brij S20 at a minimum concentration of 3. interesting finding was that the relevance of the negative zeta potential increased with Brij concentration. Generally, similarly charged particles repel each other, preventing agglomeration. As a result, similarly "charged particles" are usually found to be better segregated than "uncharged" ones . The relevance of the "zeta potential" values in this case implies that the DT entrapping micelles are evenly segregated, and have a longer stability, which is the primary explanation for DT's extended presence in the Brij micelle.