Methanol is a polar solvent which is used extensively in the process of pre-treatment of N. sativa seeds. The seeds were dried and ground using an electric grinder by adding 10mL of water to obtain a powder. 10g of it was kept for soaking in 50 ml of methanol. The mixture was placed for stirring on a magnetic stirrer for 120mins. Filtration was performed using Whatman filter paper -1 with a pore size of 110nm. Thymoquinone is a light-sensitive compound, on filtration store the yellow filtrate in a sealed and wrapped container at 4 0 celsius.