Open-ComBind has been developed as a Python package with an accompanying command-line interface for running the entire pipeline. We provide the pre-processing, docking, featurization, and pose selection as easy-to-use Python modules or a single command on the command line. After installation of Open-ComBind and its pre-requisites from our github repository: www.github.com/drewnutt/open-combind, users can run the entire Open-ComBind pipeline with the command: open_combind prep -dock -and -predict helper_ligands . csv where helper ligands.csv is a comma-delimited file of the helper ligands SMILES strings and identifiers. The user must specify the location of the raw protein-ligand crystal to use for docking. Other protein-ligand crystals can be placed in the same directory for pre-processing.