It was also necessary to validate the ratiometric sensitivity of released microchips toward GSH with the microchips detached from the surface. For this purpose, SOµC-Bdpy1 (Figure ) and SOµC-Bdpy2 (Figure )) released microchips in suspension were incubated overnight in a 5 mM GSH in culture medium, and the ratiometric signal of the released microchips was measured using the average of eight fluorescence intensity profiles across each microchip. Since the orientation of microchips in the suspension is different from the x axis projection of microscope, it is of high importance to select and focus exclusively on the surface of microchips which are in flat orientation. The average ratiometric signal for SOµC-Bdpy1 (Figure ) was ca. 1.7 ± 0.2 (ca. 50 SOµC-Bdpy1) which showed a close ratiometric consistency with the particles attached to the surface incubated in 5 mM GSH in culture medium during overnight according to Figure . Regarding the SOµC-Bdpy2 (Figure )), the ratiometric calculations also was ca. 1.3 ± 0.1 (ca. 50 SOµC-Bdpy2) which is comparable with the ratiometric signal calculations experiments of microchips on the surface in Figure .