Our major purpose in studying enzymatically created α-SeNPs is to adapt the enzymes to make enzyme/nanoparticle conjugates for use in biological EM for molecular contrast. Relatedly, a currently cutting edge method in biological imaging is 'Correlated Light and Electron Microscopy' (CLEM). Enzymatically produced α-SeNPs are visible in electron microscopy, but not light microscopy. Due to their luminescence, the metal selenide NPs produced from α-SeNPs may enable simultaneous contrast in light and electron microscopy. The use of these nanoparticles for CLEM, however, depends upon their in situ conversion not displacing the original particle.