The only target that went unmatched with the updated ILSA in this example is Target 6. To most analysts, it will be clear that Target 6 is the minor isotope of xylazine. Adding minor isotope matching to the ILSA was discussed, but deemed unnecessary for our current application spacewe have not seen many minor isomers as targets in our evaluation of seized drug evidence. It may be worth re-visiting additional targeting approaches, such as minor isotope matching or dimer matching, in application areas outside of seized drug analysis. 1 Note that output FPIE and RevMF scores using the original ILSA described in were composite values that included other metrics like the Δm/z. Here we present just the average FPIE and RevMF scores comparable to the updated ILSA (i.e., demonstrating the effect of implanting a noise filter and set mass range, and the widening of matching window when computing spectral similarity).