Co-transfection of genes coding for different enzymes was suggested for cancer therapy and treatment of metabolic diseases. But it was shown in some cases to result in the expression of only one or the other enzyme rather than the desired dual expression of both enzymes, because of the competition for the use of intracellular resources available for gene expression. Direct delivery of NGs containing defined enzymatic composition avoids the co-expression step and can thus overcome this issue. Moreover, high local enzyme concentrations within the nanogels can be of particular interest for creating or restoring complex metabolic pathways requiring close spatial positioning of several catalytic processes within an enzymatic cascade.