was made by combining ethyl cyanoacetate with thiocarbohydrazide (10) at the latter's melting point of 140-150°C. The reaction was carried out by the straightforward addition of thiocarbohydrazide (10) to the nitrile and carbonyl groups in ethyl cyanoacetate with the elimination of ethanol to produce the intermediate 20, which then followed by water elimination to give the compound 21. Compound 21's IR spectra provided two bands at 3482 and 3306 cm -1 , which are characteristic of NH2 group, and two bands at 3273 and 3206 cm -1 , which are characteristic of two NH functions. Compound 21's 1 H NMR spectrum exhibited three D2Oexchangeable signals at δ 5.65, 8.41, and 8.65 for NH2 and two NH protons, respectively, and a singlet signal at δ 7.12 for the pyrazole ring proton. Additionally, the molecular ion peak in its mass spectrum was found at m/z (%) 154.94 (39.36). The current research proceeded further to examine the susceptibility of compound 21 to αhalocarbonyl compounds. Thus, with the objective of producing only one isolable product, 2-(6amino-5H-pyrazolo[5,1-c][1,2,4]triazol-3-ylthio)acetaldehyde derivative (24), compound 21 interacted with an equimolar amount of chloroacetic acid 22 in refluxing ethanol with a catalytic quantity of triethylamine. Comparable to this, when compound 21 was combined with the 2bromo-acetamide derivative 14, the result was a single product (as determined by TLC), for which the two structures 26 and 27 as described in Scheme 5 appeared to be feasible. However, the spectral information of the reaction result precluded the other alternative structure 27 and was only compatible with (2E)-2-(6-amino-5H-pyrazolo[5,1-c][1,2,4]triazol-3-ylthio)-3-amino-N-(5methylthiazol-2-yl)acrylamide derivative (26), its IR spectrum indicated two amino groups with four absorption bands at 3306, 3273, 3204, and 3176 cm -1