For the SAMPL6 challenge which involved the 11 molecules in figure , challenge organizers encourage participants to consider the effect of water-saturation on the predicted partition coefficients. The experimental log 10 P o * /w 1 values were all positive, indicating a preference for the octanol-rich phase, with values ranging from 1.94-4.09. This is larger than the SAMPL7 range of 0.58-2.96, indicating a greater preference of the SAMPL6 molecules for the octanol-rich phase. In general, it was found that the use of water-saturated 1-octanol instead of pure 1-octanol only slightly lowered the RMSE by 0.05 to 0.10 log units as compared to experiment. Methodological differences and the choice of force field were found to have a greater impact on the prediction accuracy than the composition of the 1-octanol phase . Table provides a set of reference predictions provided by the challenge organizers comparing the use of neat 1-octanol (REF07) and water-saturated 1-octanol (REF02) using the TIP3P water model and GAFF for 1-octanol and the solutes, along with the experimental values . For this set of reference predictions, the average difference and the average absolute difference between log 10 P o * /w 1 and log 10 P o/w 1 is 0.03 and 0.25, respectively. This is noticeably smaller than observed here for SAMPL7. Additionally, for 3 of the 11 solutes we find that the inclusion of water in the octanol-rich phase de-creased the predicted octanol/water partition coefficient, indicating a decreased affinity for the octanol-rich phase. For SM07, SM08, and SM12 the decrease is 0.17, 0.45 and 0.60, respectively.