To accelerate HT experimental screening, an Opentrons liquid handling platform (OT-2) was employed (Figure , top), and the reactions miniaturised. The OT-2 deck consists of standard micro-titre plate (MTP) positions and is traditionally used in aqueous-based biological settings but was selected here as a low-cost and open-source accessible solution that was adapted to tolerate harsher chemicals. Previously, HT organic cage synthesis has only been reported using high-cost robotic platforms, arguably limiting the uptake of automation as a tool in materials discovery. The OT-2, in contrast, offers a low-cost suitable alternative. Protocols for both HT synthesis and sample preparation for characterisation were written in Python using the Opentrons open-source Python package and application programming interface (API). A general Python input script was written that included adjusted aspirate, dispense and gantry speeds, along with functions to pre-wet and swell the pipette tips and lower the maximum volumes of the pipettes -this was required to prevent damage to the pipette, uncontrolled dripping of higher density or more volatile solvents across the platform deck into untargeted wells, and most importantly, to ensure accurate and precise dispenses of the nonaqueous solvents and precursor stock solutions (see Table and Figure for further details and solvent calibrations). This script then calls upon dictionaries containing precursor stock locations and volumes with their corresponding transfer steps for each plate.