Daptomycin is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus, originally isolated from a soil sample collected at Mount Ararat, Turkey . Despite its more than 20 years of clinical use, Daptomycin resistance has been rare, though resistant strains of Bacillus subtilis, Staphylococcus aureus, and Enterococcus faecium are emerging . Daptomycin is unusual in that it is an anionic peptide that targets bacterial membranes, which are also negatively charged. This specificity is facilitated by calcium ions (Ca 2+) ), which promote Daptomycin oligomerization and mediate its interaction with bacterial cell membranes.