Surface pressure-area isotherms. Surface pressurearea isotherms of monomolecular layers were recorded at room temperature (22 °C) using a KSV NIMA KN 2002 (Biolin Scientific) Langmuir trough equipped with a teflon trough (243 cm or 555 cm 2 ) and symmetric delrin barriers. A Wilhelmy plate made of a 30 mm×14 mm piece of Whatman #1 filter paper was used to monitor the surface pressure. At first, the trough surface was rinsed with methanol. Next, Milli-Q water was used to thoroughly clean the trough. Typically, a solution of the lipids of known concentration in chloroform (or 9:1 chloroform: methanol) was slowly added dropwise onto the surface of the water using a glass microsyringe. The solvent was allowed to evaporate for at least 20 min. The barriers were then compressed at a rate of 10 mm/min and pressure-area isotherms were recorded.