Each lipid type was simulated with 100 lipids in each leaflet, in either pure, or 20% in POPC, DLPE, or SSM-CHOL membranes. The membrane builder insane was used to setup all systems, recently extended to handle both Martini 3 and 2 lipid nomenclature (). The initial box size was 8 nm x 8 nm x 10 nm and solved with water, neutralizing ions and 150 mM NaCl. Same equilibration steps were used as described in Section 3.2.1 and each system ran for 1 µs at 310 K and the first 200 ns were excluded from analysis. For comparison, available Martini 2 lipids corresponding to any of the tested Martini 3 lipids were also simulated following the same protocol. Note, some Martini 2 analysis is shown in duplicate as the same Martini 2 lipids can correspond to up to two Martini 3 lipids due to the finer acyl chain granularity (2 carbon increments compared to 4 in Martini 2).