Another assumption, now made in building protocols and unbiased fusion assays, concerns the variability of LNP morphologies, which are possibly metastable assemblies that can vary in size and structure depending on the experimental process used in their production. Integrative modeling approaches for prebuilding models seem ideal in this scenario, as precisely modeling microfluidic experimental processes used to synthesize LNPs is currently not feasible with computational methods. However, the prebuild approach requires experimental information, particularly some low-resolution data about the expected organization of the LNP core. Lack of information on the concentration of water-soluble ions inside the internal water compartments may be a concern in this approach. The self-assembly of small LNPs, as discussed in Section 2.4, highlights potential issues with ions. More experimental and theoretical studies should be conducted to clarify the amount of ions and each LNP component in different subcompartments.