96-well plate sort of 100% 6-carboxyfluorescein tagged microspheres. The FACS was calibrated using the 96-well plate set up program along with BD Accudrop beads. 6-carboxyfluorescein tagged TGbeads (2 mL) were put into a FACS sized falcon tube. 1 gate was created for the population of TGbeads. The TG-beads were sorted into wells B1-9, C1-9, D1-9 in a ½ log digression starting at 50,000 microspheres down to 5 microspheres. The 488 nm laser was used with filter 513/17 nm. All data was collected and analysed using the BD FACSJazz Sortware sorter program. The 96 well plate samples were then read on the Victor X4 plate reader (Fluorescein (485 nm/535 nm, 1.0 s).