The hypothesis on the function of this domain was corroborated by the prediction of membraneembedded residues of 178-198 (H8 and H9) by the PPM 3.0 server (Figures and). The predictor classified AcmB as a peripheral protein with Gtransfer of -12 kcal/mol. This important difference from KstD1 may also result in the concomitant stabilization of the ligands (see below). As a result, we named the Y153-R204 fragment the putative membrane-associated domain.