Leveraging these hollow microneedles, herein we report the first on-body, human dose-response data derived using an EAB sensor. In achieving this, the first in-human data for any type of continuous, affinity-based biosensor, our work has surmounted a number of key milestones in the practical, continuous molecular monitoring of analytes beyond glucose. First, the work presented demonstrates practical tools for the manufacturing and dry-shelf storage of devices employing electrochemical aptamer-based sensors. Second, we demonstrate the utility of employing simple, gel-pad skin adhesive counter and reference electrodes, which simplify device construction and testing . Finally, our design reduces the skin damage of previous (enzymatic sensor) in-vivo microneedle demonstrations, which required 10s to 100s of perforations , by employing only 3.