HPLC system (Agilent 1260) was composed of the quaternary pump with a diode array detector. The sample was eluted by 0.00625M H2SO4 solution endowing at a rate of 1.0 ml. min -1 in a reversed-phase C-18 column (4 mm ID × 125 mm in length). SA was identified at a wavelength of 210 nm. The preceding methods' findings were compared with a margin of error of less than 2%. A standard calibration graph was obtained by running different dilution of the standard SA, for the quantification of acid in the aqueous phase, that is presented in fig. . The values of R 2 close to approximately 0.99 are appearing negligible deviation within the result. The data presented in this paper is from an HPLC system. A mass balance was used to determine the acid content in the organic phase.