A major portion of urea applied for enhancing crop yield and quality is lost every year in the form of NH3, N2O, and NO3 and contaminating air and water. These losses increase the economic burdens on farmers. The reduction in N losses in the form of ammonia volatilization into the air and NO3 leaching in water are necessary for the safety of the environment throughout the world. The present study objective was to examine the combined effect of urease and nitrification inhibitors on rice crop of Faisalabad, Gujranwala, and Sheikhupura (Pakistan) to minimize the urea hydrolysis to reduce ammonia emission. It was also observed that the combination of nitrification and urease inhibitor (A1 and A2) showed better results regarding the reduction of N loss in the form of ammonia as well as emission of NO2. The highly significant (p<0.05) results were recorded from 1-14 th days when 0.50% inhibitor dose was applied. Based on results, it was detected that 6.50-6.67, 35-37 ℃, and 30 min were the optimum pH, temperature, and incubation time for enzymatic action of urease in all three districts (Faisalabad, Gujranwala, and Sheikhupura) soil with A1 inhibitor combination. In addition, Faisalabad soil showed better plant height, root length, number of tillers, and spikes as compared to other districts due to rainfall and irrigation. However, it was well documented that 25-40% of the urea is lost in the environment and causing air and water pollution. In addition to the financial impact of (AST + Nitrapyrin) by saving >10% urea, this strategy could also be adopted to clean almost 60-100% of environmental and water pollution caused by urea losses. However, the present findings provide a scientific basic recommendation on how to apply urease and nitrification inhibitors for rice crop production.