CLSM imaging was performed on a Zeiss confocal microscope (Zeiss LSM 880 Airyscan, Zeiss, Germany) equipped with an optional Airyscan unit, or a Nikon body equipped with a Re-Scan confocal unit (Confocal.nl, Netherlands). The Zeiss was equipped with a Plan-Apochromat 63x/1.4 NA DIC M27 Oil immersion objective (Zeiss, Germany) and the Nikon with a Plan-Apochromat 60x/1.4 NA DIC H Oil immersion objective (Nikon, Japan). The Zeiss confocal microscope used a 405 nm laser line at 4% (7 μW) laser power for confocal, and a 514 nm laser line at 5% (1.7 μW) laser power for confocal, and 1.5% (1.2 μW) for Airyscan. The detector ranges for CFW are 410-509 nm and for RhB 531-703 nm. The Nikon used 3% (25 μW) for the 561 nm laser line and 20% (3 μW) for the 405 nm laser line, and the detector ranges from 410-480nm for the 405nm line and 565-610 nm for the 561 nm line. The power values were measured in the focal plane of the objective.