For our second set of bootstrapping/sub-sampling calculations on the PTP1B and PDZ3 systems, we focused on the scenario of only using 10 randomly selected replicas (5 replicas from each conformation/state simulated), and instead, using a different spacing between the frames used. For the PDZ3 domain we evaluated 9 different time frame spacing scenarios between 0.1 ns and 10 ns, whilst for PTP1B, we evaluated 8 different time frame spacing scenarios between 0.2 ns and 10 ns (frames for PTP1B were initially collected with a time spacing of 0.2 ns).