Contrast within images is fundamental to their qualitative and quantitative interpretations. Objects with similar illumination source interactions within a sample produce similar detected signal, becoming camouflaged like a chameleon on a leaf. Biological samples have limited intrinsic contrast in the aforementioned imaging modalities. Therefore, an extensive set of extrinsic contrast agents and genetically encoded tools such as Green Fluorescent Protein (GFP) that produce intrinsic contrast are now available.