The antibacterial effect of silver-based materials has been extensively studied and known for a long time, and its activities have been reported to be entirely due to its release of silver ions and negligible direct influence from the shape or size of the particle . The AgNp can serve as an effective vehicle to deliver Ag ions directly to the bacterial membrane and cytoplasm by evading binding with natural ligands (which reduces its bioavailability) . Others showed that AgNp activity against E. coli and S. aureus decreases the activity of the respiratory chain dehydrogenase of both bacteria, enhances the protein, and reduces sugar leakage from the bacterial membrane by increasing its permeabilities, and forms reactive oxygen species which can damage the bacterial protein structure and its intracellular system . This also agrees with other published work that came to a similar conclusion that Ag acts through three main mechanisms such as membrane damage, production of reactive oxygen species, and cellular uptake of silver ions. .