Next, we investigated pharmacokinetics in mice receiving i.p. doses of 0.5, 2, and 5 mg/kg/d on seven consecutive days in order to select a dosing scheme for the significantly more challenging 4-weeks model of M. tuberculosis infection. Although the single 10 mg/kg i.p. dose was well tolerated in the P. multocida sepsis model, we observed severe adverse effects in the highest dose group (5 mg/kg/d) on day 2. However, seven consecutive doses of 0.5 mg/kg/d and 2 mg/kg/d were well tolerated. Based on PK results (Fig. ) it seems plausible that the toxic effect of multiple 5 mg/kg doses is caused by an accumulative effect due to the long half-life of MyxA.