We presented the capability of the CF-SMD simulation to estimate the dissociation time at equilibrium. We demonstrated that the CF-SMD simulation with the DHS model provided the dissociation rates of the NaCl, FKBP, and streptavidin-biotin systems, and the rates were comparable with those obtained from equilibrium MD simulations or an experimental value in earlier studies. The CF-SMD simulation could estimate the dissociation time over a wide range of dissociation times.