As discussed previously, zirconium frameworks have been extensively used in drug delivery studies, for anchoring biomolecules such as enzymes and phospholipid vesicles. A strong attraction of zirconium MOFs for phosphoric acid groups was observed in Zr-O nodes in UiO-66 nanoparticles. The nodes participated in Zr-O-P bond formation to capture phosphorous species. Similar cationic interaction was observed with zirconium ions and oxygens of the phospholipid headgroup in the GUV membrane that ensures stable anchoring of the lipid bilayer. We investigated two zirconium MOFs that share common chemistry but significantly different topology due to varying ligands: UiO-66 (made with linear disposed terephthalic acid) has relatively smaller particles and MOF-808 with trigonally disposed benzene tricarboxylate as a ligand. We have previously demonstrated GUV immobilization with Zr-BTDZ and MOF-808. We extend our understanding by exploring its interaction with the DOPC:DPPC:Chol lipid mixture and investigating GUV anchoring for extended duration of over 12 hours, insights that had not been reported. The results showed GUVs anchoring with both UiO-66 and MOF-808 at multiple instances using all the lipid mixtures (Figure , S4, S5), supplementary videos SV7 and SV8 showcase GUV@MOF immobilization. The trend in the vesicle diameter was the same for both zirconium MOFs where the POPC:Chol mix showed narrow GUV size distribution producing smaller GUVs (mean diameter 17 µm with UiO-66 and 18 µm with MOF-808) (Figure , S10). In terms of GUV yield, a high number of GUVs were formed in presence of MOF-808 compared to UiO-66 for all lipid compositions (Figure ). We anticipate this is because UiO-66 particles are smaller and more dispersed in the electroformation solution and would lead to more interactions with the phospholipid layers during GUV electroformation. Our study also revealed that the zirconium MOFs with benzene tricarboxylate ligand (i.e. MOF-808) demonstrated GUV binding seen at multiple sites in the imaging well and notable increase in GUV yield and compared to dicarboxylate MOF UiO-66.