Values taken for electricity use, methanol use, steam use, NOx emission factor for biodiesel, and fertilizer use are almost same in studied literature. However, there is high uncertainty over the values taken for seed yield, and life cycle span; therefore, several scenarios are analysed for parameters: seed yield and life cycle span of Jatropha. Scenario analysis is also done for the Electricity parameter as it is observed to be highly sensitive and it is expected that emission intensity of Indian power grid will decrease in coming decades due to expected increase in share of renewable energy.