We proceeded by testing how the presence of three emulsifiers would change the protein distribution at the droplet interfaces in model emulsions prepared with a high-pressure homogenizer. In the first experiment, we added phospholipids in addition to phosvitin and SDS to observe their impact on the spatial distribution of phosvitin. Again, phosvitin was labeled with a phosvitin antibody conjugated with Alexa Fluor 647. In this experiment, the absence of dyes at the interface of many droplets indicates the presence of either phospholipids or SDS that outcompete phosvitin at the droplet interface. Visual inspection of the super-resolved image of the sample qualitatively showed a more heterogeneous distribution of phosvitin at the interface of those droplets that had phosvitin present (Figure ). To assess the distribution quantitatively, we applied the segmentation to isolate all droplets' localizations in the field of view (Figure ) and calculated the RPD over all droplets. The peak analysis indicated a Arel value of 0.43; thus, we consider the phosvitin distribution at the interface in the presence of phospholipids and SDS as heterogeneous. This finding is also reflected in the RPD histogram, which shows a clear deviation from the fingerprint of a homogeneous distribution (Figure ).