The dynamic experiments were executed as a set of 6 different experimental points (Figure ) to explore a broad chemical space. Three different temperature levels (180 °C, 190 °C, and 200 °C) were investigated, with two dynamic ramp sets for each. The ester concentration was varied between 0.3 M and 0.5 M. The equivalents of amide and TBD were varied between 1.0 -2.0 and 0.25 -0.50, respectively. Product inhibition was investigated in the last experiment by adding 0.15 M (0.3 equivalents) of product to the reaction mixture. At the beginning of the dynamic experiment the system was equilibrated to steady state (for 5 min) with a residence time of 1 min. Then, a residence time ramp over 15 min was executed, from the shortest residence time of 1 min to the longest of 10 min. At the end of this ramp, the system was allowed to reach steady state again for 10 min. Then, a change for the next reaction conditions was achieved by dynamically changing all reaction parameters (residence time, temperature, equiv amine, equiv TBD, and concentration of ester) over 15 min. The resulting experimental program consisted of 4.5 h, resulting in a total time of 6 h, when combined with the initial calibration/standard addition levels.