We employed a composite model fitting approach to analyze the simulated data, allowing us to validate the matching between the input parameters used in the simulation and the values obtained through the fitting process (Supplementary Figure ) . The composite model is built from several distinct components, each of which contributes to the overall distribution of pairwise distances between localizations at the droplet interface. The composite model is composed of parts describing the rotational symmetry, repeated localizations, and arrangement of proteins. The rotational symmetry component represents the isotropic distribution of proteins at the circular droplet interface. The localization precision component represents the influence of localizing the same protein. The arrangement of proteins component accounts for proteins that are adsorbed closely, causing the protein localizations to become unresolvable in the clusters.