CRISPR/Cas-based diagnostics leverage the precise targeting capabilities of the CRISPR/Cas system, particularly variants such as Cas9, Cas12, Cas13, Cas14 and Cas3, to recognize and bind to a target nucleic acid sequence followed by, cleavage used to generate a detectable signal. The key mechanisms of various CRISPR/Cas-based platforms developed for disease diagnosis have been described in Table and details of individual detection platforms are summarized in Tables . -detect and cleave ssDNA without requiring the presence of a PAM 360 -they are significantly more compact (400 to 700 amino acids) than Cas12a (about the half size) -Cas14, unlike Cas12, has lower tolerance to nucleotide mismatches between sgRNA and the target template, which greatly reduces on-target activity of Cas14 allowing use of Cas14 to detect SNPs in DNA 302 ▪ HARRY: Highly sensitive aptamerregulated Cas14 R-loop for bioanalysis 363