To assess the difficulty of the D-ribosome project as a whole, we have assigned each protein a predicted difficulty rating -Easy, Medium, Hard, or in the "Danger Zone" -based on our own experience and precedents from literature. The Max Path score is a good gauge of synthesis complexity, as increases in this score correlate with protein size as well as diversity of ligation junctions (Figure ). Thus, we can use Max Path as a simple rule of thumb (easily counted by eye) to initially judge synthesis difficulty.