Chagas disease (ChD) or American trypanosomiasis is caused by the parasite Trypanosoma cruzi (T. cruzi) and transmitted in 90% of cases by triatomine vectors (more than 150 species are involved) ; other mechanisms of transmission are transplacental route during pregnancy, by transplants of infected organs, by transfusions of blood products, as well as by the consumption of food or beverages contaminat ed with the parasite, with the vector or its feces . In addition to biological factors, the macrophages, generating reactive oxygen and nitrogen species such as superoxide anions, peroxynitrite, myeloperoxidase and hydrogen peroxide , these chemical molecules have high reactivity and their main consequence is the oxidation of biomolecules such as carbohydrates, lipids, nucleic acids and proteins; causing irreversible and severe damage that makes it impossible for trypanosomatids to replicate. T. cruzi confronts this environment with a sophisticated and efficient antioxidant machinery consisting of pathways linked between a thiol-dependent redox system: triparedoxin (TXN), an oxidoreductase that regulates low molecular weight dithiol-dependent oxide-reduction reactions, and NADPHdependent trypanothione reductase (TR), an enzyme that regenerates the reduced form of trypanothione .