Mass Spectrometry Metabolomics on the Fraction Library: Upon thawing fractions A -H (10 mg/mL in DMSO), 2 μL of fractions A -D and E -H were combined in a LCMS vial containing 90 uL of 50% ACN/H2O. An aliquot (2 μL) of this mixture was analyzed by LC-qToF under the following conditions: Phenomenex Evo C18 column (2.6 × 100 mm), 0.3 mL/min, hold 30% ACN + 0.1% FA/70% H2O + 0.1% FA for 1 min then gradient to 100% ACN + 0.1% FA over 12.5 min and held for 1 min before reequilibration at 30% ACN + 0.1% FA/70% H2O + 0.1% FA for 4 min. MS data were collected using an Agilent 6530 q-ToF in positive mode using Agilent MassHunter software. MS1 data were collected from 100 to 2,000 m/z. MS/MS spectra were collected using data-dependent acquisition, for which the top three abundant ions in the MS1 scan were selected for fragmentation, while employing dynamic exclusion to avoid fragmenting the same ion more than twice in a 2 min range. In addition, an exclusion list was employed that contained features that were present in the control extract of the three-nutrient media. Collision-induced dissociation was applied using a linear formula that applied a higher voltage for larger molecules [CID voltage = 10 + 0.02 (m/z)]. MS scan rate 1/s, MS/MS scan rate 3/s, source gas temperature 330 °C, gas flow 34 L/min, nebulizer 35 psig, Global Natural Product Social Molecular Networking (GNPS) on fraction library: The raw MS files were converted into mgf by MSConvert and analyzed through the following GNPS platform. A molecular network was created with the online workflow () on the GNPS website (). The data were filtered by removing all MS/MS fragment ions within +/-17 Da of the precursor m/z. MS/MS spectra were window filtered by choosing only the top 6 fragment ions in the +/-50 Da window throughout the spectrum. The precursor ion mass tolerance was set to 1 Da and a MS/MS fragment ion tolerance of 0.5 Da. A network was then created where edges were filtered to have a cosine score above 0.65 and more than 6 matched peaks. Further, edges between two nodes were kept in the network if and only if each of the nodes appeared in each other's respective top 10 most similar nodes. Finally, the maximum size of a molecular family was set to 100, and the lowest scoring edges were removed from molecular families until the molecular family size was below this threshold. The spectra in the network were then searched against GNPS' spectral libraries. The library spectra were filtered in the same manner as the input data. All matches kept between network spectra and library spectra were required to have a score above 0.7 and at least 6 matched peaks.