The step-wise decomposition of the flux network is carried out by first identifying the strongest pathway (i.e., the pathway with largest minimum flux among all possible pathways). This process is continued until the network is fully decomposed to be free from A to B pathways. For detailed discussion on TPT, readers are directed to Metzner et al. the center of the polar head group (cf. Figure and S7 -S8). Within the subsequent coarsegraining of the microstates using PCCA++, four nicely-separated macrostates are identified for both solution conditions based upon the projection of the first two TICA components (IC1 and IC2) (Figure ). These are labelled NP1-4 and P1-4, respectively for the NonPol and Pol solutions. Using the stationary probabilities (Table ), the relative free energies for these states are found to be within 1.6 kJ/mol; the four states are essentially energetically degenerate within the TICA space and statistical sampling obtained. Interestingly, the mean first passage times (MFPTs), which is the expected time required for a conformation within a macrostate to reach another macrostate, indicates varied barriers between these states.