To illustrate the approach, here we show the case of KAXQIL, where the barrier detection was performed using an energy step 𝛿𝐸 of 0.3 kJ mol - . A single symmetrically unique type of channel was identified in KAXQIL, with a minimal energy of -44.3 kJ mol -1 -the various channels shown in Figure are all symmetrically equivalent. The code detected a single merge that resulted in a fully connected component within the channel. This merging occurred at an energy of -25.7 kJ mol -1 (as depicted in Figure ), indicating that the estimated activation energy is 18.6 kJ mol -1 with an uncertainty of 0.3 kJ mol -1 (due to the energy step used). 1