values have been reported for only C12-and C14-BACs in the literature, and they agree with the EC50 values obtained in this study within a factor of 2-4. 8, 19 EC50 values from the solvent spiking and passive dosing methods from this study agree well for C14-BAC (Figure ). However, the EC50 values from the passive dosing method for C16-BAC and C18-BAC are lower than those from the solvent spiking method.