A simple protocol was used to test the newly obtained coarse-grained parameters for disaccharides: 500 steps of steepest descent minimization followed by 100 ns equilibration in the NVT ensemble and 1 µs NPT production run (T = 300 K, pressure = 1 bar). The LINCS algorithm was used to constrain bonds in the ring moieties, enabling the use of a 20 fs timestep, unless otherwise stated. Electrostatic interactions were treated using the reaction-field approach with a cutoff of 1.1 nm and a dielectric constant of 15 . The same cutoff was used for van der Waals interactions with the implementation of a shift function for the potential.