The final re-equilibrated state of the GUVs often resulted in remaining excess membrane area being taken up in membrane buds at the GUV surface. These small buds often have a high fluorescence intensity, suggesting that they may be multilamellar structures (Fig. ). The estimated composition of the MO and DOPC mixture in these buds would favour lamellar structures, based on previous small angle X-ray and neutron reflectivity experiments (note, the MO to DOPC ratio was about 45 mol%; see also discussion below). Neutron scattering studies have revealed an initial rapid adsorption of intact LCNPs at the DOPC bilayer interface, followed by exchange of lipids. Furthermore, SAXS confirmed the strong interaction between LCNPs and DOPC vesicles including unilamellar and multilamellar that initially leads to changes in the size of the unit cell of the cubic phase before transition to the lamellar structure (DOPC/MO molar ratio of 0.01-0.18).