The results for P11 with single-use containers (SU) demonstrated a broader range of cannabinoid concentrations across the conditions. Notably, the SU operation for 20 seconds at 2,000 rpm was associated with the highest percentages of THCA and THC among the SU mill conditions. In contrast, the SU mill's operation for 10 seconds at 2,000 rpm was most effective for achieving the highest percentages of CBDA and CBD.