(Biotage conical 0.5-2.0 mL, Part No. 352016) and probe sonicated (Sonics Vibracell VCX 230, ¼" probe, 50% amplitude, centered and tip at 15 mm from bottom of tube) for 15 min in an ice bath. After, the contents were transferred to a 1.5 mL microcentrifuge tube and centrifuged in a fixed angle rotor at 20,000 rcf, for 1 h, at 4 o C. The supernatant was transferred to a new 1.5 mL centrifuge tube and the pellet was discarded. The suspension was then re-centrifuged for an additional hour at 20,000 rcf, 4 o C, in the same rotor. The supernatant was then removed, and 2 nd pellet discarded. To account for possible differences in preparation, multiple 1 mL preparations were run this way and combined to create one bulk solution (10 preparations total). An aliquot of this bulk supernatant was diluted (10X) and absorbance measured at 632 nm (NanoDrop One C) was used to estimate stock concentrations. The bulk supernatant solution was diluted to 100 ppm with 0.1M NaCl and stored at 2-8 o C until use.