The study confirmed the presence of 17 amino acids in arthropod hemolymph, with glutamic acid being the most abundant in spiders (5.08 ± 0.11a) and cystine the least abundant in crickets (0.02 ± 0.01e). The arthropods studied could serve as valuable sources of amino acids such as glutamic acid, arginine, and aspartic acid for use in animal and human nutrition. Additionally, the hemolymph of butterflies, beetles, scorpions, crickets, and spiders could be explored as culture media for Trypanosoma brucei brucei propagation in vitro, contributing to foreign exchange conservation.