Recently, additive manufacturing and 3D printing techniques have gained a lot of attention for their use in the rapid manufacturing of thermoplastic microfluidics -usually composed of PLA and ABS, due to technological advancements that have yielded effectiveness with complex designs and improved precision. Additive manufacturing of microfluidics is especially useful for the fabrication of innovative monolithic and heterogeneous microfluidics in which multiple layers of chips with different functionalities are stacked together to obtain a small 3D platform exhibiting superior performance. These systems have showed high applicability within complementary metal oxide semiconductor (CMOS)-integrated optical and electrical sensors, microfluidic cooling systems on 2.5D or 3D integrated circuits (ICs), and some other lab-on-a-chip applications .