Upon receipt of the plated compounds, we determined that four compounds were incompletely dissolved. These compounds were removed from further consideration, leaving 1,024 for testing. Among the 1,024 compounds, 701 compounds were in the top 1,500 from the RF-C and 705 were in the top 1,500 from the Similarity Baseline, including 382 compounds selected by both models. All 1,024 compounds were tested in duplicate at a concentration of 33.3 µm to reveal 412 hits. Our AMS hit criteria required that both replicates exceeded 50% inhibition and the compound passed a pan-assay interference compounds (PAINS) filter (Section 4.8).