Unfortunately, this disease has no cure at present . Regrettably, existing therapies have adverse side effects and are expected to impose increasing socioeconomic problems on society and a significant challenge for biomedicine . To surmount the existing problems, worldwide P.D. patients are suggested to take medicines in the form of herbals . These natural products have been experimentally documented to possess numerous biological properties such as antioxidant, anti-inflammatory and anti-apoptotic activities. In vitro and in vivo studies have further renowned for the utility of natural products in various preclinical models of neurodegenerative disorders such as Alzheimer's disease, Epilepsy, Parkinson's disease . In recent years studies have been proven that, Celastrol is a quinone methide tri-terpenoid, which is isolated from the roots of the Celastraceae family has been reported as a potential candidate to have neuroprotective properties in animals' models of Parkinson's, Huntington's, Alzheimer disease . It possesses a vast range of biological activities including antioxidant properties. Studies have been reported that the antioxidant activity of Celastrol is powerfully more effective than alpha-tocopherol . So far, there have been very few reports are available on the efficacity of Celastrol against rotenone-induced P.D. model of SH-SY5Y cells especially on neurodegenerative diseases. reported about bioactive compounds of Tripterygium wilfordii belongs to the Celastraceae family of neurodegenerative diseases. The present study aimed to understand the efficacity of Celastrol in neuroprotection of SH-SY5Y neuroblastoma cells.