Living cells have evolved various mechanisms to acclimate to environmental changes. The ability of an organism to become accustomed to new ambient conditions plays a vital role in keeping the cells alive. Bacterial cells employ multiple adaptive mechanisms under various environmental stresses. For example, heat shock and osmotic pressure influence eukaryotic cell membrane fluidity and gene expression. Bacteria respond to environmental conditions through both short-term and long-term adaptations. Short-term responses need to occur immediately to avoid lipid phase transitions due to temperature fluctuation, osmotic stress, or toxic substances. Gram-negative bacteria are known to show a short-term response to environmental stress by isomerizing unsaturated cis lipid tails to trans through the cis-trans isomerase (Cti) enzyme. This alteration causes increased packaging of acyl chains in trans conformation which increases the rigidity of the phospholipid membrane and leads to an increase in membrane stability under stress. Pseudomonas putida, a Gram-negative bacterium, is resistant to harsh conditions like high pH or nutrient scarcity. Due to its fast growth in simple bacterium culture, P. putida has drawn researchers' attention to its use in the industry, from synthesizing biopolymers to degrading xenobiotic substances. For instance, polyhydroxyalkanoates (PHA), a biocompatible polymer produced by P. putida, has various applications in biodegradable packaging and tissue engineering, and P. putida can incorporate multiple exogenous aromatics into its metabolism for industrial use. Besides polymer production, this bacterial strain is an excellent host for expressing genes from other bacteria.. As an example, P. putida can produce some complex natural products of myxobacteria which are applied as high-values pharmaceuticals. The tractability for engineering P. putida makes it an excellent candidate for a starting point to investigate cis-trans isomerization, particularly as cis-trans isomerization is heavily regulated in Pseudomonas species which may be exploited to change membrane properties by design.