Classification based on multivariate analysis of global clustering results. The relative distribution of pixels in each cell belonging to each of the 17 clusters was computed and used as input for linear discriminant analysis (LDA), which was used to aid in the visualization of the global clustering results at the cell level and to classify the cells according to their respective leukemia subtypes. Three main subsets were considered, namely "AMLs+ALLs", comprising the full dataset of 315 cells belonging to the nine leukemia subtypes; "AMLs" for the six AML subtypes (165 cells); "ALLs" for the three ALL subtypes (150 cells). LDA was performed on each subset separately and then followed by leave-one-cell-out cross-validation (more details in the Supplementary Information).