In order to increase the sensitivity of these assays, a number of alternative signal transduction mechanisms have been demonstrated. Examples include the use of femotliter (fL) detection volumes , or signal conversion with nanomaterials . Nevertheless, the traditionally used absorption-based detection methods still represent the gold standard , especially in low budget settings. As such, ELISAs are often restricted by their sensitivity as well as their dynamic range and a method to extend the dynamic range of this assay system would be highly beneficial.