Figure shows DSC thermograms of free ctDNA and ctDNA with COSAN at a [COSAN]/ctDNA(bp)] ratio of 0.13. As expected for genomic DNA, ctDNA has a complex melting profile with several transitions because ctDNA consists of long strands with many domains, which melt independently. The thermogram of ctDNA with COSAN shows the same shape and position as the melting profile of free ctDNA. The values of the maximum temperature of the molar heat capacity curve (Tmax) are the same for both free ctDNA and ctDNA with COSAN (Figure ). Furthermore, by analyzing the thermograms using Gaussian models in the NanoAnalyze program, we deconvoluted both melting profiles into five separate transitions (Figure ). With COSAN, the melting temperatures (Tm) of each transition showed no significant differences with free ctDNA (Table ). Based on these results, COSAN most likely does not affect the melting transitions of DNA and, thus, should not intercalate into DNA.