T-azoJSiR640 was weighed, dissolved in methanol and aliquoted to 1.5 mL Microtube Black for Shading (WATSON ® BIO LAB). The solvent was evaporated and the tubes were stored at -25℃. The samples were reinstated in an appropriate solvent before use. The probe solution was prepared individually for each experiment and not reused. T-JSiR640 was solved in DMSO and stored in Safe-Lock Tubes 1.5 mL at -25°C. The concentrations of stock solutions were calculated from the by ε values.