The selection of scaffold for DNA origami, such as the m13mp18 viral genome, depends on size and complexity, and staple strands can be purchased as oligonucleotides or produced by oligo synthesizers. DNA origami yield depends on cation concentration, typically using buffers with varying Mg 2+ concentrations. After that, self-assembly is done through thermal annealing, with duration based on complexity. Here, denaturing polyacrylamide gel electrophoresis (PAGE) purification is used for dynamic structures. Stepwise assembly may be employed for integrating materials or creating hierarchical structures. Achieving high-yield complex DNA nanostructures at a constant temperature would be valuable. DNA origami structures assemble through base stacking interactions between terminal bases, promoting geometric matching and surface contact for shape complementarity in hierarchical assemblies. To ensure reproducibility in DNA origami assembly, several key aspects need to be considered. Optimization of annealing procedures and cationic strength is crucial for target product yield and avoiding by-products. Purification methods, such as size-exclusion chromatography, ultracentrifugation, gel or filter purification, and polyethylene glycol (PEG) precipitation, should be chosen based on factors like yield, duration, and residual contaminants. Purifying and concentrating intact origami nanostructures to high concentrations of greater-scale structures, which typically involves synthesizing a high quantity of staple strands, is still the significant barrier to their practical use in biological applications. The stabilization of DNA origami structures is dependent on the suitable storage temperature and cationic strength, with options including thermal stability, photo-cross-linking-assisted stability, lyophilization, and the use of block copolymers for long-term storage. The success of the self-assembly process is determined by utilizing ensemble methods, such as UV-visible spectroscopy, gel electrophoresis, fluorescence spectroscopy along with circular dichroism. Although providing information about the overall characteristics of the assembled molecules through these techniques, individual details may remain unresolved . DNA origami structures provide a surface for the precise positioning of chemical species at nanometer distances. Each nucleobase can be chemically modified, allowing the precise placement of molecules along the helical axis with a spacing of 0.34 nm. Methods such as single-molecule fluorescence microscopy, transmission electron microscopy (TEM), and atomic force microscopy (AFM) have been applied to examine and characterize DNA origami formations experimentally . It appears that purification remains a significant challenge that hampers the practicality and productivity of DNA origami production methods. This challenge arises from various factors, including the actual efficiency of oligo synthesizer instruments achievable in practice and the need for strict controls to be implemented throughout the process, such as preventing moisture from entering the system. One possible approach to address these challenges could be changing the basis of production methods beyond chemical synthesis to include biological synthesis, similar to the high-throughput and adequately efficient procedure that naturally occurs in living cells. For instance, the exploration of enzymatic tools or methods inspired by the process through which the DNA primase (Fig. ), a ribonucleic acid (RNA) polymerase type, catalyzes the synthesis of short RNA molecules during DNA replication such that the enzymatic process can be utilized for staples and short strands production.