In this continuation, the inhibitory effect of captopril molecules on the nanozymatic activity of peroxidase-like nitrogen-doped carbon dots was investigated. In this regard, the carbon dots were synthesized via a simple solvent-free method and then characterized for their FL properties and enzyme-like activity. Thereafter, the effect of captopril molecules on their nanozymatic activity was evaluated by calculating their nanozymatic activity in the presence and absence of captopril. The results reveal that the relative activity of nanozymes was inhibited by increasing the inhibitor concentration and finally reached 23% of its initial activity, revealing a strong inhibitory effect of captopril on the nanozymatic behavior of peroxidase-like nitrogen-doped carbon dots.