The sample-suspension mixture was vortexed and incubated for two hours at 4°C, then centrifuged for 10 minutes at 3214 x g, and 50% of the total supernatant was collected and dried under a gentle flow of nitrogen. After drying, the sample was resuspended in water in half of the original sample volume to maintain the sample dilution for the C18 RPC+/-analysis. The remaining supernatant was subjected to C8 RPC+ to assess the lipid species present. Sham control samples were also prepared.