Synthesis of third derivative of sugar ester-based mangiferin, using raw mangiferin with isobutyric acid and tert-Butyl alcohol in the presence of immobilized lipase lipozyme in a water bath with thermostat control vibration shaking table at 60 °C for 24 hours, the reaction mixture was further concentrated with the help of a rotary evaporator that provided a residue that was subjected to purification with column chromatography using chloroform : methanol as eluent to provide the desired compound 3.