Electron diffraction data using 200 kV electrons was acquired at a Talos F200C TEM equipped with an Apollo direct electron detector with a frame frate of 60 Hz . During data acquisition, sets of 30 frames were integrated (2 frame per second effective rate) and binned to 2048x2048 images to produce frames in the movies used for further analysis in MRC file format. For each type of sample, well-diffracting crystals were located using diffraction mode settings focused away from the back focal plane to view a projection of the sample while delivering negligible fluence, and circumventing need to repeatedly reconfigure the lens system between imaging and diffraction mode. For data acquisition, the microscope was operated in microprobe diffraction mode with a parallel beam, illuminating an area approximately 5 microns in diameter. All data was collected using a 100 micron selected area aperture, with a projected diameter of approximately 2 microns on the plane of the specimen. Diffraction movies on stationary crystals were acquired without any rotation of the TEM sample stage over a 5 minute period, for the small molecules studied, and a 2.5 minute period for AVAAGA, thiostrepton, and proteinase K.