Sensor 2 with flow. To test the pore holder within the fluidic chip, an initial assessment of the baseline current was carried out to illustrate the pore was wet and able to conduct a current. A silicon membrane, either SiN or SiO2, could be placed into the screw thread along with a small amount of ethanol to remove air bubbles from the top and bottom o-rings. Once the pore was wet, the holder was attached to the fluidic device (Figure ) and the ethanol replaced with an electrolyte. A typical baseline current is shown in figure . Regardless of flow rate, voltage and particle concentration, no translocations were observed when 400 nm particles flowed into the fluidic device, and this observation was consistent across multiple SiN and SiO2 pores.