After shock, 1 mL of Middlebrook 7H9 broth with ADC and leucine was immediately added into the cuvette. The culture was incubated for 3 hours at 37°C and 10% portions of the culture were plated onto a Middlebrook 7H10 agar plate supplemented with ADC, 20 μg/mL kanamycin, 20 ng/mL anhydrous tetracycline and 50 μg/mL leucine. The plates were sealed by parafilm and incubated at 37°C. Seven days after electroporation, the overall editing efficiencies were evaluated using the method described in the Editing efficiency evaluation Section of the experimental procedures and a single colony was cultured in 5 mL of 7H9 broth supplemented with ADC and leucine at 37°C for two days in the absence of kanamycin. Then the cells were plated onto Middlebrook 7H10 agar plates supplemented with ADC and leucine in the absence of kanamycin.