After sequencing, samples were decoded and analyzed using the DELTA toolkit . The features with a PolyO score of 4 are defined as significantly enriched features. Concurrently, the performance of these features through a historical analysis of hundreds of targets was also applied to identify and remove potential frequent hitters. Compounds on the enriched features with a sequence count >5 are defined as the positive dataset. The nega(ve dataset was assembled using compounds similar to posi(ve hits (2X the number of posi(ves), and through random sampling based on molecule ID (8X the number of posi(ves).