Macrophages are one of the leading cells in response to the trauma and support in healing of all bone types . Their depletion leads to an increase of membranous bone formation in the periosteum and decrease in the intertrabecular space, which suggests a deeper influence on the healing process. They fulfill an important role in onset and resolution of inflammation. Two primary macrophage types are involved in this process: the inflammatory M1 macrophages, which predominantly respond to infections, and the anabolic M2 macrophages, which are critical for tissue regeneration .