A 15x15x15 Å (15x) grid centered at the R4-TrD interface was employed to optimize the target cavity of the parent candidates described above. A wider grid of 45x45x45 Å (45x) centered to the PyMol / centerofmass, surrounding most of the pre-fusion gpGVHSV homotrimer (~ blind-docking) was employed to confirm affinity and R4-TrD target of the ADVconformers. Because of the large numbers of children, only the best-fitting ADV-conformer of 9 possible per child was selected for further studies. The corresponding output ADV-conformer docking-scores in -Kcal/mol were converted to ~ nM affinities by the formula, 10 9 *(exp (Kcal/mol/0.592) ).