For each sample, about 1 μg of total tryptic peptides were analyzed on a Waters M-Class UPLC using a 15cm Thermo EASY-spray column (75µm inner diameter; 3µm particle size; p/n ES900) coupled to a benchtop Thermo Fisher Scientific Orbitrap Q Exactive HF mass spectrometer. Peptides were separated at a flow rate of 400 nL/min over a 65 min gradient, including sample loading and column equilibration times. Data was acquired in data independent mode using Xcalibur 4.5 software. All raw data were analyzed with Spectronaut software version 17.2 using directDIA analysis methodology and searched against Uniprot P35236 (HePTP). Oxidation of methionine, protein N-terminal acetylation, carbamidomethylation on cysteines, and modification by the treated compound on cysteines were set as variable modifications. Trypsin/P was set as the digestion enzyme.