Physical and biological degradation are two key modes of biodegradation in polyesters. In our study, biological degradation occurs through the action of microorganisms (lipase enzyme) operating under in vitro biological conditions by employing orbital movement and maintaining a temperature of 37°C. As the copolymers primarily consist of PBS, which contains ester bonds, the choice of a lipase enzyme becomes pivotal due to its water-soluble nature and its ability to hydrolyze ester bonds in water-soluble substrates . Numerous studies have explored the enzymatic effects of lipases on PBS-based polymers using enzymes derived from various sources. The choice of lipase species has been demonstrated to influence experimental outcomes, with factors such as enzyme source and specificity playing crucial roles .The lipase from Pseudomonas cepacia is recognized as a commercial enzyme, available in both soluble and immobilized forms. Its widespread recognition is attributed to its thermal resistance and tolerance to a broad spectrum of solvents and short-chain alcohols . These attributes make it a robust enzymatic agent for polymer degradation studies, providing stability under various conditions and allowing for versatile applications. Lipase was similarly employed in other study involving PBS-based copolymers containing PEG-like subunits . This consistency across diverse research reinforces the relevance of our method and highlights the recurring utilization of lipase for its unique attributes in such contexts. While our study primarily employed lipase for the enzymatic degradation study, it is noteworthy to mention other enzymes with specificities for ether bond-cleaving, which can contribute to PEG degradation. One such enzyme is 'diglycolic acid dehydrogenase,' which has been identified in PEG-utilizing symbiotic mixed cultures . Additionally, studies reveal enzymes such as PEG carboxylic acid or diglycolic acid dehydrogenase, PEG acetaldehyde lyase, and alcohol dehydrogenases, contribute to the intricate biochemical pathways facilitating the degradation of PEG . The degradation study was conducted over a period of 20 days, with sampling at 6 different time intervals. This approach allowed for a comprehensive understanding of the degradation behavior of the copolymers, with a PCL polymer included as a control.