Articles were first screened by title, followed by abstracts and finally full text. Articles selected were peer-reviewed English publications quantitatively examining the TSD of blood. The following articles were excluded: i) commentaries, editorials, reviews, opinions; ii) articles examining the TSD of other biological fluids, iii) articles providing a qualitative assessment of blood TSD in which there were no convertible summary statistics (r, R 2 , F), iv) articles in which blood was not deposited on a substrate, v) articles not in English; and vi) articles where the full text was not available. Data from retained articles were extracted and inserted into a summary table. Data collected, if reported, included summary statistics (r, R 2 , F, t, p-value), the number of biological replicates, the class of biomolecule examined (whole blood and cellular elements, genetic material, proteins), the analytical technique used to analyze the timewise trends, blood source (e.g., human, bovine), the temperature, substrate porosity, length of time series, and author affiliation (country).