Charged liposomes were prepared as described under Section 2.3.1 but by adding oleic acid to the lipidic mixture (1:1 molar ratio with respect to the phospholipids) prior to preparation of the thin film. The film was rehydrated to a concentration of 6.25 mg/mL of phospholipids. Following preparation of the thin film, teriparatide loaded charged liposomes were obtained by dehydration rehydration. A physical mixture of the empty liposomes (800 μL), teriparatide (20 μL, 10 mg/mL) and sucrose (10 μL, 100 mg/mL) was prepared and freeze-dried (dehydration), then rehydrated as described under Section 2.3.1.