In order to assess the environmental impact of the enzymatic synthesis process conducted under different conditions, namely in bulk and diphenyl ether, the E-factor values were estimated using equations ( ) and . Reagent calculations were performed to obtain 6 g of PBA-DLA copolyesters. The reaction consisted of 1,4-butanediol (21.0 mmol, 1.89 g), diethyl adipate (23.7 mmol, 4.80 g), and dilinoleic diol (2.77 mmol, 1.50 g) in diphenyl ether (96.22 mol, 16.38 g), which yielded 5.28 g of PBA-DLA_S (88% by weight). Alternatively, the reaction conducted in bulk (without diphenyl ether) resulted in 5.72 g of PBA-DLA_B (95% by weight). Additionally, calculations considered the amount of tetrahydrofuran (THF) used for CAL-B purification for subsequent reaction cycles to ensure possibly comprehensive results.