The pre-steady state and steady-state kinetics aimed at establishing the kinetic mechanisms of the enzyme were collected using a stopped-flow spectrophotometer SX20 (Applied Photophysics). The solutions were treated with argon for several minutes to provide anaerobic conditions. All reported concentrations are the final values obtained after mixing and diluting the reactants. All measurements were performed in triplicate. Data were collected using Pro-Data software and processed using OriginPro 2019b software.