For the majority of all three troponin subunits, phosphorylation results in very small perturbations of the dynamic stability of the cTnI, cTnT and cTnC subunits, with the standard error overlapping throughout the sequences. There is, however, a significant change in the flexibility of the NcTnI residues (1-30) with phosphorylation resulting in an approximate 33% to 50% reduction in RMSF values. The RMSFs for G159D, similarly, do not change significantly with phosphorylation and are not significantly different from wild-type.