The LC-vDMS-SIM/MS methods were compared to an LC-MRM/MS method, and in all cases, interassay precisions were lower than 12.2% and inter-assay accuracy better than 115%, despite the reduced analysis times in the LC-vDMS-SIM/MS methods. Compared to flow injection analysis approach, the LC dimension remains important as it allows preconcentration of the analytes and reduces matrix effects demonstrating the benefit of LC and DMS for developing high-throughput methods as applied here for DoA analysis. MRM. SIM and DMS parameters of drug of abuse and their metabolites (n=13).