Second, we measured KiSSim's discriminating power between DFG conformations by comparing fingerprint distances between structure pairs in DFG-in/in, DFG-out/out, and DFG-in/out conformations. For this analysis, we used the distances based on only the spatial fingerprint features to exclude the eight physicochemical features and thereby to focus on conformational information. While the distributions for the three categories are similar when considering all kinases (data not shown), they differ when split by kinase as shown exemplarily for the BRAF kinase in Figure , indicating that the fingerprint can discriminate between DFG conformations. We conducted this analysis for other kinases with sufficient structural coverage for DFG-in and -out conformations and observed the same for CDK8, EphA2, MET, and p38a (see details in notebook 62 ).