Each experiment continued for a total of t = 750 s, with the vesicles being lysed with detergent after a total of t = 600 s. The experiments are longer than the HPTS transport assay due to the increased time required for transport equilibration to occur once the transporter has been added. Hill fitting of the transport data at t = 600 s enabled both EC50 values and Hill coefficients (n) to be obtained for thorough analysis. Conforming to the previously established Cl -transport activity trend, the highly fluorinated transporters 1c, 2c, 3c, and 4c displayed the highest activity in the series (Table ).