At 310 K, the LNPs in inverse hexagonal phase (Lipid 10 and MC3) contained ∼12 mass% of water (Table ) while Lipid 2-based LNP consisted ∼16 % of water (Figure ). Heating and cooling the systems led to LNPs with ∼14 % of water in inverse hexagonal phase and 24 % in case of multicompartmental vesicle of Lipid 2. The original ∼690 Cl -beads stayed mostly inside the LNP, without significant differences between the systems (Table ).