Current knowledge about the impact of protein coronas on the interaction of NPs with biological membranes, and hence the potential impact on biomedical functionality and cytotoxicity that arise, still presents considerable gaps. The myriad of parameters influencing the non-specific biomolecular coating of nanomaterials leads to highly complex and variable structures formed under different experimental conditions, which makes it difficult to extract unified conclusions . Understanding protein corona formation and their influence on NP stability, reactivity and biocompatibility is therefore an extremely challenging task requiring the combination of different experimental perspectives. In this context, reductionist studies represent a first step to provide fundamental information on structural and dynamical aspects of protein corona assembly, their influence on NP stability and their interactions with biological membranes.