In a typical example, 130 g of dried C. asiatica leaves were extracted with ethanol-water 80:20 (v/v) (3 x 250 mL) at room temperature. Evaporation of the solvent under reduced pressure yielded 22.0 g raw extract. The raw extract was added to 200 mL ethanol/water 1:1 and 60 mL aqueous 20% NaOH, and heated at 70 °C for 4 hours. The hydrolysate was acidified with 60 mL 5% HCl to pH 4.0. After standing overnight, 14.2 g of solid was precipitated. Both the raw extract and the solid were analysed for MA, AA and TA by HPLC.