Fully hydrated multilamellar vesicles (MLVs), composed of POPC and CHOL with the latter present at 050 mol-% with 5 mol-% increments, were prepared for small angle X-ray scattering (SAXS) experiments using rapid solvent exchange as described previously. This avoids the precipitation of CHOL crystallites at high concentration, 56 yielding non-phase- separated samples up to 50 mol-% CHOL content. Lipids, purchased from Avanti Polar Lipids (Alabaster, AL, USA), were used as dry powders without any further purication.