To prepare the inocula, large particles were first removed by sieving each sample through a 0.25 mm mesh. Due to the differences in physical properties and relative ease of trickling, inocula for AS and CM were prepared differently than AD. AS and CM were sieved through the 0.25 mm mesh and used directly as inocula. For nutrient media preparation, the remaining quantity of AS and CM, along with the AD, were centrifuged at 4800 rpm for 10 minutes and filtered through a 0.125 mm mesh. The AD filtered through 0.125 mm mesh was also used as an inoculum. 36.0 ± 1.0 52.5 ± 1.1 42.4 ± 1.0 30.2 ± 0.5 31.9 ± 1.4