1 % (w/v) carrageenan suspensions were prepared and treated in a water bath at 60 °C until the polysaccharides were completely dissolved. For partial enzymatic hydrolysis, 250 µL of the carrageenan solution were mixed with 200 µL of water and 50 µL of enzyme solution. The samples were incubated for 24 h at 37 °C with constant agitation. After incubation, the enzymes were inactivated at 95 °C for 10 min. For comparison, carrageenan solutions without the addition of enzyme (250 µL of 1 % (w/v) carrageenan solution, 250 µL of water) and enzyme solutions without the addition of polysaccharide (50 µL of enzyme solution, 450 µL of water) were prepared.