Electrochemical analysis includes Polarization and Electrochemical Impedance Spectroscopy (EIS) what both of those are the most important electrochemical tests in fuel cell researches. Polarization analysis provide significant information related to cell efficiency such as power density (mW.cm -2 ), voltage (V) and current density (mA.cm -2 ). Impedance spectroscopy that counted as the most popular analysis techniques in electrochemistry and electrical engineering, presents valuable information about structure of material, resistance of cell, constant phase element and others that helpful to explain how cell efficiency change in different ways. Equivalent circuit of cell structure is necessary for extraction the parameters from impedance plots . Therefore, by mention of experimental details of cell structure such as anode, cathode, anode and cathode support, electrolyte conditions and test properties can be claimed that the dataset provide remarkable and essential information about DBFC performance for researchers who want to use empirical data for study on experimental fuel cell setup, simulation or machine learning analysis to build an analytical and a mathematical model based on resulted data.