To construct the VEBD, the variant target identifiers were mapped to ChEMBL bioactivity data based on assay-target pairs. Duplicated data from several assays for the same variant target were joined into one single point by dropping data with questioned validity, considered lowquality, and calculating the mean pChEMBL value or the most common activity flag. This resulted in 1,870,748 compound-target pairs across 6,777 targets, of which 25,259 contained variant targets -736 with undefined variants -and the rest were WT. The ChEMBL 31 annotated set was merged with the fraction of the Papyrus dataset version 5.5 originating from the Christmann subset, keeping only targets with at least one variant defined for the follow-up analysis of variant-annotated bioactivity. The final combined VEBD for bioactivity analysis contained 455,839 compound-target pairs across 335 proteins, of which 25,086 data points represented data on variant proteins. Of these, 22,992 compound-target pairs originated from ChEMBL 31, 672 from the Papyrus Christmann subset, which were not present in ChEMBL, and 1,422 from both sources. In the following sections, we explore in detail the VEBD.