Curiously, this design initially resulted in seemingly well-folded yet circular bundles (Supplementary Fig. ). Kinetic traps occasionally occur in scaffolded self-assemblies and may be responsible for these circular structures. The same design folded with a different 3246 bp fragment of the 60R21N-9kbp plasmid folded with good yield (70%, n = 76) into a straight square-lattice bundle (Fig. ). A more complicated square-lattice eight-fold bundle with up to four neighbours per folded segment is shown in Figure . It is assembled from a 7296 bp linearized scaffold and 100 TFO staples, which mostly have 15 nt recognition domains. Most adjoined segments again have a crossover density of two crossovers in each 60R21N section, but this is halved between two central folds to conserve staple length. The design folded successfully but its yield was poor (<10%). Similar to the 12-fold honeycomb bundle, malformed structures were mostly split or partials and TEM analysis again revealed a significant extent of internal disorganization. The eight-fold bundle was also assembled with the full-size plasmid as its scaffold (Supplementary Fig. ).