'View' mode was used for generating montage overviews of the center working area of a grid. The typical montage was configured to sample an array of 7x7, 155x magnification images. These images were recorded with an exposure time of 0.25 s, 2x binned, yielding sufficient resolution to identify potential crystallites of interest (Supplementary Figure ). Record mode was used to corroborate the positions of crystals identified in the grid montage overview. To achieve this, it was configured to acquire real-space images at 2500x -4300x magnification, with the C2 lens condensed to illuminate the same area as would be sampled by a parallel beam when sampling diffraction. The selected area aperture, not under the control of SerialEM, was inserted as needed to confirm each crystal remained eucentric within its bounds throughout the entirety of the tilt range used for data collection (+/-50 °).