Table . Binding kinetics and affinity of anti-idiotypic antibodies to therapeutic. All anti-IDs were used at a concentration of 100nM. Table . Summary of epitope binning data indicating there is no antibody response with the addition of a second anti-ID. for sandwich anti-ID ELISA screening. B) Representative plate map for labeled anti-ID screen. Six Sulfo-TAG-anti-IDs were used per plate with a single biotinylated-anti-ID to measure the concentration of therapeutic over >2 orders of magnitude +/-soluble antigen. The non-specific binding (NSB) +/-soluble antigen was also measured. C) PK assay formats screened. Six PK assay formats were screened: three homogenous formats, two modified assay formats, and one sequential assay format. D) Scoring matrix for rank ordering of anti-ID pairings. E) Cumulative total score of two concentrations of anti-ID pairings across all PK assay formats. Scores were established for each anti-ID pairing/PK assay format per concentration. The scores of all six PK assay formats per anti-ID pairing were tabulated. The top two pairings across all PK assay formats are indicated by (*). F) Cumulative total score of PK assay formats across all anti-ID pairings. The scores of all anti-ID pairings per PK assay format were tabulated. The top PK assay format is indicated by (*). (Logo) WAND is a software tool with graphical user interface to automate experiment set-up and data analysis by incorporating information on several assay formats, detailed error handling for specific formats and automated data analysis to minimize human error. The user specifies different assay parameters, conditions, and scoring weights, generating comprehensive reports as excel sheets for further analysis. (A) The file selection panel allows WAND to access and process plate reader files. (B) The antigen selection and screening type panels allow the user to specify the appropriate experimental descriptors for a given plate reader file. (C&D) The experimental details, and subsequent panels, are displayed such that prior specifications dictate the inputs available that ensure proper descriptor entry. (E) ID entry is performed in a separate window which allows users to input the appropriate name of reagents. (F) Dynamic scoring entry allows for users to modify the interpretation of plate reader files such that desired analytical values are given higher pertinence post analysis.