The surface was blocked with an injection of 200 μL of 1 M ethanolamine, and 100 μL of the diluted model analyte at a proper concentration was injected at a speed of 20 μL/min to interact for 240 s (Step I). After dissociation and stabilization (Step II), the detecting mAb at 2 mg/mL was sampled to observe the binding signal of the antibody-analyte-antibody ternary complex (Step III). The surface was regenerated with an injection of regeneration buffer (10 mM HCl, pH 2.0) at a speed of 150 μL/min between each analyte injection, and the chip was washed in 10 mM HCl (pH 2.0) to remove impurities. Data were collected at a rate of 5 Hz, single referenced with blank injections, and fitted to a 1:1 interaction model using Trace Drawer analysis software.