In order to expand DNA-based protein delivery to strong local concentrations of delivered protein, here we propose to incorporate a large number of active protein molecules into three-dimensional DNA nanogel network. We have recently reported a new type of DNA nanogels where the intramolecular crosslinking was achieved not by hybridization or covalent DNA crosslinking, but by noncovalent specific interactions between a multibiotinylated DNA (bt-DNA) and streptavidin. Here, we show different strategies for functionalization of these nanogels with two model cargo proteins: green fluorescent protein (GFP), and alkaline phosphatase (ALP) -enzyme which has been proposed for replacement therapy against the hypophosphatasia disorder. And we investigate the delivery of the resulting functionalized nanogels, containing up to 100 µM local protein concentration, into cells by using lipidic nucleic acid transfection agents, known for promoting endosomal escape after internalization of lipoplexes by disrupting the membrane integrity.