Glyphosate-based herbicides (GBHs) are mixtures of chemicals rather than a single compound (Fig. ). The commercial product includes "active" ingredients that are subject to strict regulations for home and agricultural use. As a result, the main focus on human exposures has been limited to glyphosate, the active ingredient of GBHs. Little information is available about additives in the formulation. They facilitate glyphosate absorption and perfusion into plants. The most often employed co-formulants are polyethoxylated tallow amine (POEA) surfactants. Recent studies suggest that POEA additives in GBHs, rather than the glyphosate itself, exert significant endocrine-disrupting effects in human cells. However, there is an enormous knowledge gap about human exposure to POEA co-formulants including their toxicokinetics and appropriate biological matrices for biomonitoring.