The molecular formula of yeast is assumed to be CH1.61O0.56. While fed-batch and draw-fill operation modes lead to higher yields and titers compared to standard batch operation, the mode of operation in this study is limited to batch due to the high nitrogen content of the substrates. Nitrogen serves as the limiting nutrient for growth and its absence drives microbes to accumulate oil during the production phase. The C:N ratio (by weight) of the oilcane bagasse hydrolysate and juice were measured to be 40 and 54, respectively, which are far lower than the optimal for the production phase (~120 C:N ratio): as a result, continuous feeding would stop the microbes from entering the production phase. The starting C:N ratios of the substrates are closer to the optimal starting C:N ratio for batch operation (70). It may be possible to supplement with hydrolysates from other feedstocks with lower C:N ratios (e.g., wood chips or pure sucrose) to enable other modes of operation, but these were not considered in this study due to the complexity of the design and logistics.