To determine the ability of CV-N to cause shedding of the S protein complex from SARS-CoV-2 spike trimers upon treatment, SARS-CoV-2 WT pseudoviruses (50 ng of p24 worth of pseudovirus) were treated with various concentrations of CV-N and ACE2 (human recombinant ACE2 protein, 10108-H08H, Sino Biological US Inc., Wayne, PA,) for 24 hours at 37°C, before washout of lectins and ACE2 using a similar protocol to that described in the washout experiment. Untreated pseudovirus controls were incubated and washed under identical conditions. After washing, the flow-through from the washes of "CV-N-treated" pseudoviruses and "ACE-2 treated" pseudoviruses were tested for S1 content in each treatment using the western blot protocol as described.