The recall of the hotspots and non-hotspots for sixteen different systems are illustrated in bar-chart, (Figure .). A recall value of 1.0 means 100% accurate match between the hotspots categorized by FMOPhore on apo-structure to hotspots categorized on the holocomplexes of the same protein target. In all systems the recall for hotspots is found to be above 0.8 (80% of the binding site residues are correctly categorized as hotspots using the apo-structure scanning) except for the HIV which recall 0.78. Some systems showing a recall of 1.0, e.g., JAK-2, PKB, PPARs, and p38. Other systems, such as Aurora-A, Beta-secretase, Biotin, CDK, and Ephrin-A2, demonstrate high recall values for hotspots of more than 90% accuracy. For non-hotspots, the recall rates are slightly lower in comparison to hotspot counterparts across all systems. This is mainly due to the FMOPhore scan on apo-structures which can detect more residues in the binding site vicinity and categorizes them in the four different categories, where such residues can be missing from being categories in the holo-complexes as they might have been not