These results are in good agreement with neutron reflectometry data previously reported for PDMAEMA brushes. Hence Tran and co-workers reported comparable swelling ratios for brushes with dry thicknesses in a range matching the thickness of our thinnest coating (near 10 nm) and intermediate grafting densities (0.23 chains/nm 2 ) . In this study, thicknesses calculated from neutron reflectometry data were extracted from the normalized first moment of the brush volume fraction profiles (extracted from SLD profiles). Therefore, although methodologies used for quantification differed in our study, comparable conclusions and swelling behaviours were extracted. In addition, Tran and co-workers also observed a gradual increase in solvent content in upper brush layers, with profiles that were strongly dependent on the pH of the environment. As in our data, solvent contrasts do not enable to confirm whether the origin of such profiles is simply a reflection of homogenous brush morphologies, or whether protonation states may also vary, dependent on the depth position within the brush. However, we note a sharp increase in monomer volume fractions close to the substrate at neutral pH, comparable to brushes poorly swollen in methanol. Overall, the swelling of brushes was also found to reduce with increased dry thicknesses.