Depth analysis (Figure ) mirrored these results, showing membrane penetration by the first 12 residues of the protein. Beyond the N-terminus, W238 and F241 are unique, in that they may also be found beneath the membrane surface. These data indicate that the protein primarily contacts the membrane along two helices in the DHDDS domain, one stretching from L11 to K21, and the other from T236 to L245.