It is significant that the liposomes immobilized into the pores of AAO can be released intact by ultrasonication. In this study, electron-assisted dielectric microscopy (SE-ADM) , which is a powerful tool for observing various biomolecules in water with little radiation damage and highcontrast imaging, was used to evaluate the structures of the released liposomes by ultrasonication. As shown in Figure , the spherical-shaped particles were observed for the released liposomes. Pseudo color images and three-dimensional maps of the liposome particles showed similar appearance to previously reported liposome imaging using scanning dielectric microscopy by G. Gomila et al. (Figure ). We found negligible change in the particle diameter and shape for the released liposomes compared to control liposomes before immobilization into AAO membranes. The results strongly suggest that liposomes maintained their vesicle structure during immobilization and release on AAO membranes. The examination of nanoparticle tracking analysis revealed the size distribution for the released liposomes is comparable to that for control ones (Figure and Table ), which supports the observation by SE-ADM. Therefore, the liposomes released from AAO pores can be intact.