To investigate the animal toxicity of hydrogel, in addition to measuring the toxic effect of hydrogel and drug-containing hydrogel on important organs such as kidney, liver and spleen, the function of these organs is also evaluated. For this purpose, at day 12, 2 ml of blood was taken to check hematological parameters including White blood cells (WBCs), Red blood cells (RBCs), hemoglobin (HGB), Hematocrit (HCT) and Platelets (PLT), Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and biochemical factors including total protein (TP), albumin (ALB), calcium (Ca), phosphorus (ph), blood urea nitrogen (BUN), creatinine (CREA). Moreover, to evaluate the morphological changes in main organ of animals including kidney, liver and spleen, the tissue of these organ was harvested and first fixed in 4% paraformaldehyde, dehydrated, embedded in melted paraffin, cut into thin slices with a microtome, and stained with H&E and was observed under an optical microscope.