Samples of Co(II)-porphyrin were prepared by crushing commercially acquired crystalline powders of the compounds between glass slides and dusting onto TEM grids. Samples of Cu(II)serine and Zn(II)-histidine were prepared by crushing macroscopic crystals into powder between glass microscope slides and dusting onto TEM grids. Samples of biotin, thiostrepton, and Zn(II)methionine were prepared by suspending recrystallized material (in ethanol, for biotin and thiostrepton, and water, for Zn(II)-methionine).TEM grids were prepared by dropcasting 2 μL of crystals in suspension onto the grid and wicking the excess solvent away until dry. Samples of AVAAGA were prepared by harvesting hanging drops filled with crystals, diluting two-fold in water, and dropcasting 2 μL onto TEM grids and wicking excess solvent away until dry. To compare the degree of beam-induced reorientations in dry samples to frozen-hydrated ones, cryoEM samples of the AVAAGA peptide were also prepared by dropcasting crystals diluted in water onto R2/1 Cu Quantifoil grids, then blotting off excess solvent and plunge-freezing at an FEI vitrobot. CryoEM samples of proteinase K crystals were prepared by dropcasting crystal suspension diluted tenfold in 0.1 M Tris HCl pH 8.0 onto R2/1 Cu Quantifoil grids, then blotting and freezing at a vitrobot. Frozen-hydrated samples were stored under liquid nitrogen prior to examination at the TEM.