As Figure illustrates, the implementation of the heterogeneous sample analysis method on walk-up LCMS has decreased the instances of clogging, naturally resulting in increased instrument availability and users' satisfaction. This method led to savings in time resource by allowing chemists to continue their work without interruption. Additionally, this has led to a significant savings in resources for our Analytical team, therefore allowing these chemists to focus on value-added and project-based activities. To assess the impact on an instrument by instrument basis, Table provides details on the number of samples run across a 50 month period on each system (Instruments nominally labelled 1-6), as well as the number of clogging events per month prior to and after the implementation of the heterogeneous sample analysis method. demonstrates that in 2014 (the last full calendar year without implementation of the heterogeneous sample analysis method on any instrument), 54% of the service calls (of which there were in excess of 208) were to address clogging issues. This number was truly staggering and represented a resource drain on the Analytical team. For 2015, which represents a transitional year based on the fact that all the instruments were converted to incorporate the heterogeneous sample analysis method during this time (in total the instruments spent ~ 40% of the year without back-flush), we saw 89 clogging events, which was a reduction of 120. For 2016, the first year that all six instruments had the heterogeneous sample method implemented, we observed a precipitous drop in clogging events leading to a steady state situation of approximately six clogs per year since, this represents a 97% reduction in clogging rate since we modified our operations to incorporate the new methodology. Figure provides an alternative perspective on the impact of the heterogeneous sample analysis method by depicting the increased robustness of the walk-up LCMS systems. The plot shows a 30-40 folds improvement in the performance of the instruments from 2016 to 2018, compared with 2014 which was used as the baseline for the comparison. In addition, the rinsing of the needle seat through the injection port has significantly reduced sample carry-over, which often proves to be an issue in a walkup environment. This has enabled us to shorten the needle washing step, reducing the injection cycle time by 30 seconds, which leads to an increase in throughput efficiency of 25% and 17% for the two-and three-minute methods respectively. This in turn allowed us to further improve operational efficiency via eliminating the requirement for replenishing wash vials manually with clean solvent.