The sign of 𝛥𝜖 ; %&%' depends on the shape of the orbital 𝜑 ; (𝑟 ⃗) and the distribution of the polarization charges {𝑞 ! " }. Here, we qualitatively analyze the case for a large molecule with charge separation (Figure ). Based on the CPCM formalism, the polarization charges {𝑞 ! " } have the opposite sign to the adjacent solute charge distribution. In a zwitterionic peptide, the C-terminal carries a negative partial charge, so the surrounding {𝑞 ! " } are positive. Hence, a MO localized around the C-terminal has 𝛥𝜖 ; %&%' <0 and gets stabilized by CPCM (𝜑 : in Figure ). In contrast, a MO localized around the N-terminal gets destabilized (𝛥𝜖 ; %&%' >0) because of the surrounding negative {𝑞 ! " } (𝜑 7 in Figure ). The CPCM stabilization of a delocalized MO is between these two extreme cases.