As the DCvC of azines was reported to require acid as a catalyst, we wondered if we could u7lise pH as an addi7onal s7mulus to ac7vate anion transport. For this study, we used liposomes with preincorporated TT (1 mol%), but now at pH 7 (225 mM NaNO3, 5 mM HEPES). Then, 5 mol% AA was added, and no transport was observed (Fig. ). The remaining liposome suspension was divided into two parts. The first part remained as a control sample at pH 7. The second part was acidified by addi7on of dilute nitric acid to reach pH 5. Then the anion transport in both samples was studied afer 2h. The acidified sample showed clear transport of Cl -(Fig. ,iii), comparable to previous results obtained in liposomes originally prepared at pH 5 (Fig. ). The control sample remained unchanged with no transport observed (Fig. ,ii). Indeed, even afer 24h no ac7ve TA was formed at pH 7 (see Fig. ). These results show that we can control the azine metathesis inside the membrane externally by changing the pH of the environment, which is relevant in biological contexts.