Segmentation analysis was performed on the SEM images (Figure ), and the centroids (𝐶𝑥, 𝐶y) were mathematically calculated based on the center of mass of the pixels constituting the particles (see Section S5, Supporting Information). The centroid of the confocal microscopy image was determined by 2D Gaussian fittings. The centroids from the confocal and SEM images were overlaid to calculate the particle shift observed in SEM relative to confocal imaging.