The TOF MS scan on the Synapt G2-S was performed in both positive ESI mode (ESI + in negative mode, we decided to measure protonated species utilizing positive ionization mode for our analysis. This choice was based on the previously reported studies and the fragmentation observed in positive ionization mode offers a more straightforward pattern for acyl-CoA analysis. Table presents the observed m/z of all the targets with their identified retention time in the HRMS method.