Impact of different MS1 isolation window resolution in ZenoTOF 7600 system was also investigated. The charge-state distribution of light chain-1 payload (LC1) and heavy chain-3 payloads (HC3) of ADC1 is shown in Figure . 1800 m/z was chosen as a generic precursor ion with a wide isolation window to quantify both LC1 and HC3. The m/z peak closest to 1800 m/z is 1761.4 m/z (z=14) for LC1 and 1792.7 m/z (z=30) for HC3. Both peaks are not the most abundant peaks in the charge distribution of light or heavy chain. Nevertheless, we could dissociate the payload from both LC1 and HC3 with 1800 m/z as precursor ion and achieve adequate MS2 product peaks for quantification using open MS1 resolution (Figure , Figure ). With open resolution more ions enter collision cell compared to low resolution, which can result in MS2 saturation at higher concentration. This demonstrates the application of a more generic approach, which requires minimal method optimization for specific ADCs, which will further simplify the workflow. If a more sensitive method is desired, using more abundant m/z for precursor ion should be considered.