Incorporation of complex lysophospholipids into supramolecular assemblies. As 10:0 lyso G>P is known to self-assemble in aqueous solution, we used dynamic light scattering to evaluate the presence of aggregates in the lysophospholipid mixture before and after the dry-state reaction with EA. Prior to heating, two populations of supramolecular assemblies (4.8 nm and 124.1 nm average diameter), consistent with micelles and small vesicles, respectively, were detected. Intriguingly, upon heating at 80°C for 24 hours, only one population of aggregates (19.2 nm average diameter) was observed (Fig. ). The DLS data, and the absence of a precipitate, suggest that the newly formed amphiphilic 10:0 lyso GPEs integrate readily into the pre-existing supramolecular aggregates, impacting their size and distribution. Further studies will shed light on the ability of complex lysophospholipids to self-assemble into membrane-like structures or modify the physicochemical properties of preexisting primitive compartments.