where one should recall that [E0] is the mass concentration of the enzyme. Besides, as explained in several preprint presentations , given very accurate initial rates under well-defined quasi-steady-state assumptions, which may be either of the following: rQSSA, sQSSA, or tQSSA, , initial rates that follow a polynomial can enable the determination of the kinetic parameters, including the KM, as desired in this study instead of going through several plots. Such an equation in its simplest form is given as: