Euclea natalensis root extracts are rich in naphthoquinones, terpenoids, and other phenolic compounds that present C=O in the structure . Therefore, the appearance of this peak in uncalcined hydroxyapatite nanoparticles confirms the presence of extract molecules on the surface of hydroxyapatite nanoparticles. Additionally, peaks at 2845 and 2929 cm -1 are observed in non-calcined hydroxyapatite with the template and in the extract. These correspond to symmetric and asymmetric stretching of CH,