An Agilent 1220 Infinity II HPLC equipped with a variable wavelength detector (VWD) set to monitor 220 nm absorption was used for separation and characterization. HPLC hardware (including pumps and heaters) used were stock equipment only. The column used was a common octadecyl silica column (Agilent Poroshell 120 EC-C18 2.7µm 3.0x50 mm) which was held at 50.0±0.8°C for the duration of the experiments. Manual injection volume was fixed at 5 μL by injection loop. The flow rate was set to 1.00 mL/min with a set maximum pressure of 400 bar. See Table for the gradient elution program used.