An insert holder was created to help seed cells onto the dECM insert and house them in a 12-well plate, as detailed in Figure . Figure demonstrates the assembly of the dECM insert into the 3D-printed insert holder device, with the bottom insert holder and a top hollow lid. After a circular insert was placed in the holder, a silicone O-ring was placed onto the insert before the hollow lid was screwed into the insert holder via the printed threads.