Next, we explore the stability of the designed helix to changes in the backbone equilibrium bond-bending angle (θ B,o ) and backbone equilibrium torsion angle (φ B,o ) through a series of REMD simulations. Along with the fixed parameters specified in Section 3.1, the following force field parameters were used: σ S = 1.556, ε S = 3.33, k a = 100/rad 2 , and k t = 3.33. We also applied the symmetry constraint that 2θ S,o + θ B,o = 360 • , a simplification that we study further in the subsequent section.