In general, extensive analysis of a high number of diverse polyphenols in different plants and fungi species is limited to date, especially as numerous polyphenols exist (Fig. ). To measure a multitude of polyphenols simultaneously, liquid chromatography (LC) coupled to mass spectrometry (MS) is the analytical platform of choice. Several studies of plants and fungi, such as white button or king trumpet mushroom, exist, however typically a targeted LC-MS approach was applied. Few studies involved an untargeted LC-HRMS workflow , although this approach would allow to investigate analytes for which reference standards are not (or hardly) commercially available .