The FE-SEM analysis was conducted for the nanoparticles at a voltage of 20 kV as seen in Fig. .1 and 2.2. Furthermore, Fig. .1 was captured at a magnification of 8000x at a horizontal field width of 37.30 µm and a working distance of 10.7mm. Likewise, Fig. .2 was captured at a magnification of 1200x at a horizontal field width of 0.25 mm and a working distance of 10.7mm. Both the images showcased a cluster of nanoparticles which were structured and reduced due to the presence of phenolic compounds and saponins present as phytochemicals in the Camellia Sinensis var. Assamica tea dust. Regardless, further in the study, a closer magnification of the individual ZnO NPs would be conducted and analyzed through TEM. The biosynthesis ZnO NPs were analyzed under EDX spectroscopy. Apart from the primary presence of Zinc and Oxygen, the nanoparticles in the medium also showed a presence of carbon, as evident in Fig. . The presence of carbon and other organic matter could be attributed to the phytochemicals which remained post the biosynthesis process. This would mean that the particles need to be rinsed thoroughly before being prepared in a nanofluid for the antibacterial activity analysis. An EDX graph, as seen in Fig. , was also obtained for the sample, showcasing the presence of Zinc, Oxygen and Carbon. The percentage weight of Carbon was 57.6%, Zinc was 11.4% and Oxygen was 31.4%. As most of the carbon was present outside the sample, the ratio of Zinc to Oxygen still indicates a greater presence of Oxygen in the nanoparticles. The peaks for Zinc were visible approximately at energies 1.2 keV, 8.7 keV, and 9.7 keV. The peak for Oxygen was visible at an energy of 0.8 keV. Elements analysis was carried out once more in order to get better findings, and arrived with similar results. The presence of carbon impurities around the ZnO NPs should be addressed before the antibacterial assessment.