Commercial vendors have collections of this type of building blocks, for example, Enamine (12,000 fragments), ChemDiv (74,000 fragments), Maybridge (30,000 fragments), and Life Chemicals (65,000 fragments). The Center for Research and Advancements in Fragments and Molecular Targets (CRAFT) has made available its synthetic database of compounds and fragments at . CRAFT is a Brazilian inter-institutional collaboration between the University of Sao Paulo and the Federal University of Goias. A major goal of CRAFT is to advance drug discovery, with a special emphasis on neglected infectious diseases as well as emerging diseases such as bacterial and viral infections. CRAFT has compiled a fragment library containing structures based on new heterocyclic scaffolds and compounds derived from NPs. All the fragments in the CRAFT library were obtained experimentally, making them pharmacologically attractive. Molecules generated from the CRAFT´s fragments are designed to be synthetically accessible. The goal of this study was to analyze the contents, properties, and chemical diversity of fragment libraries obtained from two large natural product libraries (COCONUT 2.0 and the most updated version of LANaPDB), chemical synthesis (CRAFT), and commercial fragment libraries (Enamine, ChemDiv, Maybridge and Life Chemicals).