Afterwards ATP consumption and NMP formation was analyzed using the luminescence-based assay and data were compared to standard HPLC analytics. Using the luminescence-based assay NMP formation was calculated based on ATP consumption. The determined NMP formation (Figure ) and ATP consumption (Figure ) were very comparable for both methods used. The deviations between both methods were between 0.49 to 7.53 % (Table ), with the difference being highest at low (deoxy)NMP formation. For Ado, Guo and Urd there was up to 9 % ATP consumption detected by the luminescent assay while no or only very little NMP formation was observed by HPLC. The susceptibility of the assay to error has been observed