Chadsia grevei and Cnestis palala extracts presented similar chromatographic profiles, only one extract from each species (C. grevei roots bark and C. palala roots, respectively) was selected for isolation. We performed gradient optimization to maximize the resolution in the chromatographic area of interest using analytical HPLC coupled to a photodiode array (PDA) detector (4.6 x 250 mm I.D., 5 µm, C 18 column). The obtained conditions were transferred to semi-preparative HPLC (19 x 250 mm I.D., 5 µm, C 18 column) coupled to dry-load injection (See Sup. Fig. to 9) using a geometrical gradient transfer . Since all targeted compounds share a characteristic UV-PDA chromophore, their isolation could be easily monitored using UV detection alone. Following this process, compounds of interest were isolated in a single high-resolution semi-preparative chromatography step. This led to the isolation of 9 compounds from Cnestis palala, 11 from Chadsia grevei, 17 from Pachyrhizus erosus, and 9 from Desmodium heterophyllum. This represents a total of 41 different pure NPs (Figure ), some being isolated in multiple extracts (see Supp. Table for the details about the originating extract(s) for each compound). isoflavones/prenylated isoflavones, and 11 rotenoids). For rotenone , the ring nomenclature has been added to ease the structure-activity discussion.