Despite these efforts, our understanding of LNP structure remains incomplete, with various factors such as cargo type, lipid formulation, manufacturing technique, pH, and buffer composition still needing comprehensive exploration . Proposed models for LNP organization range widely-from multilamellar structures and nanostructured cores to homogeneous core-shells, worm-like models, inverse hexagonal phases, bicontinuous cubic phases, and even "bleb" compartments for LNPs containing mRNA . Among these, the inverse hexagonal model is frequently mentioned for smaller cargoes such as siRNA or polyA RNA, while the 'bleb' compartment model is often proposed for LNPs carrying mRNA .