Finally, by adjusting the concentrations of these components and the duration of UV light irradiation, the gel mesh size was optimized to effectively lock smaller nanoparticles in place. We specifically tailored the gel mesh size to match the dimensions of the nanoparticles. To validate this, we conducted single-particle tracking (SPT) experiments using a multiplane widefield microscopy system (Figure ), which allows for 3D rapid tracking of particle diffusion. The SPT experiments demonstrated that particles with a size equal or larger than 23 nm are effectively immobilized within the gel mesh network (see Section S3, Supporting Information). To evaluate the FRAME workflow, optical matter (OM) consisting of 1 µm polystyrene microparticles was formed by focusing a 1064 nm laser beam at the glass/solution interface (Figure ). Once the OM reached the desired size and arrangement, the UV-LED was turned on to effectively lock the structure in place.