Finally, the further effects of the tested compounds were studied in vitro via measuring their effects on the intracellular NO and cell migration alongside their cellular uptake. While NO enhanced the migration of TNBC cells, via its scavenging, this behaviour can be controlled with multiple effects of the different compounds on cells whether NO was introduced extracellularly or intracellularly. Hence, our work demonstrates a proof of concept for a new approach targeting the modulation of cancer cell migration via NO-scavenging. For that, some of the introduced compounds can be candidates for further in vivo studies utilizing cancer models. Meanwhile, in the long term, by following the same methods for hemin modification we may be able to enhance the NO-scavenging efficiency of hemin, while modulating its physicochemical properties in aqueous solutions. We are currently investigating these approaches, and testing the in vivo activity of certain hemin derivatives will be our focus in the future studies. Ultraviolet-visible absorption spectra (UV-vis) were scanned using a Biologic MOS-500 spectrometer.