In the second process, a solution of the peptides is allowed to evaporate at room temperature. The evaporation causes an outward capillary flow that localizes a high concentration of the peptide and the salt ions at the perimeter of the droplet, which in turn forms coacervates (Figure and Video S1). These coacervates coalesce in a similar fashion to those formed in solution (Figure and Video S2). The same solidification behavior can be observed for the coacervates that form during evaporation. Additionally, when the solution is placed on a hydrophobic substrate such as parafilm (contact angle 106 °), which lowers the spread of the droplet, allowing the solution to age for a longer duration before complete evaporation, a higher intensity of the fluorescence of the peptide can be observed and the spherical shape of the coacervates is distorted (Figure ). This further implies that the coacervates solidify as they age, beginning from their outer surface. When mixed with a fluorescent dye, lines can be observed where two or more droplets coalesced (Figure S3B and Video S2). This also indicates the formation of a semisolid outer shell that allows for coalescence but does not behave like a liquid, similar to the FRAP results in Figure .