The calculated EC50 value (k) will be correctly assigned to the concentration of the transporter (in mol%), which exhibited 50% of the total (100%) chloride efflux observed in the study. However, fitting the dataset of a transporter that does not reach 100% efflux, possibly due to poor transportability, issues with selectivity or solubility (see Troubleshooting section), will produce incorrect results. Instead, using non-linear curve fitting with Equation (11) in conjunction with the EC50 value, which has been set as a derived parameter (Equation ( )), the correct EC50 can be calculated: