As the use of AAV vectors grows, the need for full-length AAV particle separation methods that can be used within good manufacturing practice (GMP) settings increases. Liquid chromatography mass spectrometry (LC-MS) is increasingly utilized for the characterization of AAV VPs, with hydrophilic interaction liquid chromatography (HILIC) and reversed phase (RP) chromatography being the two LC separation phases most used . Complete separation of VP1, VP2, and VP3 is desired for comprehensive full-length AAV VP characterization and effective determination of VP ratios.