To 20 mL of a solution containing alcohol (10 mM) in MOPS buffer (50 mM; pH 8) supplemented with MgCl2 (5 mM) were added 80 mg of Au/CaO. The reaction mixture was then placed on a rotary shaker (revolving tube, thermoscientific, USA) at 60 °C for 24 h. The carboxylic acid formation was monitored by 1 H NMR. Then, ACL was added to the reaction mixture at a final concentration of 0.15 mg.mL -1 , along with AMFC (5.5 mM final) and ATP (50 mM final), as well as MgCl2 to maintain a final concentration of 5 mM. The final volume of reaction was of 2 mL. The solution placed at 60 °C for 24 h. Products formation was followed by H NMR (Figures ).