Besides, 5b facilitated early differentiation measured by expression of differentiation markers involving CD14 vs. CD11b in HL60 cells, and CD133 vs. CD11b in Mutz-2 cells (SI Figure ). In this line, 48 h exposure of 5b to K562 cells significantly reduced the colony-forming capacity (SI Figure ). To further evaluate the efficacy of 5b on leukemic cells, we used the zebrafish xenotransplantation model (Figure ). MOLT-4 cells were transplanted into zebrafish embryos at 32 hours post-fertilization (hpf).