The KEGG pathway of T. maritima in comparison to the reference pathway was analyzed (purine metabolism: tma00230 and pyrimidine metabolism: tma00240). All enzymes involved in the nucleotide pathway were chosen for gene synthesis. Furthermore, the genome of T. maritima (accession number: NC_023151.1) was analyzed for proteins being able to phosphorylate either nucleosides, NMPs or NDPs.