Molecular imaging based on fluorescent probes is assumed to be a sensitive technique providing online fluorescence sensing of GSH. Although these probes have made great progress, there are still a number of challenges, including water solubility and aggregation-caused quenching (ACQ), which limits the long-term tracking of the intracellular GSH levels . Due to the limitations of fluorescent probes, most of the reported works related to the recognition of intraocular GSH concentration are based on qualitative evaluation, and long-term tracking of intracellular GSH level has not been considered in these studies. On the other hand, nanostructured materials have attracted considerable attention owing to their exceptional properties, becoming integral components in the development of highly sensitive chemo-and biosensors, which they might sort out some of the inconveniences of the use of molecular probes . However, despite these advancements, concerns persist regarding toxicity, real-time, and long-term studies of intracellular GSH detection. Moreover, the use of non-reversible nanomaterials complicates efforts to conduct thorough studies on the interactions of nanomaterials within biological systems, hindering their ability to achieve reliable quantitative results. Additionally, the challenge of effectively eliminating nanomaterials from cells further underscores the need for comprehensive research and development in this field.