Previous reports of cellular imaging using g-Odots have evaluated nanoparticle cytotoxicity, brightness within cellular matrices, and efficacy of long-term particle tracking. However, the subcellular locations where g-Odots may localize remain unknown. Therefore, we investigated whether g-Odots would localize within lysosomes by using a commercially available blue-emissive dye that targets lysosomes, which we could readily spectrally resolve from the red-emissive g-Odots.