Although TPP primarily focuses on DDA workflows, it does support library-free analysis of DIA data via the DISCO tool. DISCO reads mzML files containing the instrument-produced spectra and uses signal processing approaches to isolate the fragment ions in the multiplexed MS2 spectra that correlate with precursors in the MS1 and write the results to new mzML files that may then be searched with standard DDA search engines and downstream tools, including target-decoy analysis. This provides a comprehensive analysis of DIA data without the need for building a spectral library first. It is somewhat less sensitive than library approaches, but not limited by currently available libraries, and is complementary to library-based approaches. The most recent version of DISCO in TPP 6.2.0 also supports ion-mobility mass spectrometry such as from the Bruker timsTOF, and allows the extraction of precursors and spectra acquired with an ion mobility dimension.