effects of the binding of two nucleotides, ATP and 2'-deoxy-ATP (dATP). We determine that ATP binding to SERCA, compared to dATP, induces differential allosteric effects. The most likely information pathways from the bound nucleotide to the calcium binding domain are also predicted using our MI estimator in combination with network analysis tools on the SERCA pump, which differs based on the bound nucleotide. Netsci is shown to be a useful program for the estimation of MI and GC within general datasets, and for the analysis of intraprotein communication and information transfer, in particular.