While a priori knowledge of possible boost coordinates is required when using msesMD, the use of glycosidic torsions, as previously noted by similar locally biased methods, seems to be appropriate. The form of the biasing potential used here appears to be effective for these carbohydrates, only introducing a slight amount of noise as seen in free energy profiles sLe a and sLe x when the number of boosted dihedrals increases (Figures and). In conclusion, the msesMD method appears well suited as an intuitive efficient tool for the exploration of oligosaccharides and other similarly sized systems, providing important physical insights into solution conformations for understanding of function and potential design of ligands.