However, they are lacking information on the product and on the time course of substrate or product concentrations. PyEnzyme was used to add the product and time course data to the EnzymeML document (see SI 3.4). By a single function in the API, the time course of substrate concentrations was simulated from the kinetic parameters for initial concentrations from 0 to 0.5 mM for a time interval of 200 seconds to visualise kinetic behavior and study the effect of kinetic parameters