3) enabling specific recognition and binding of biomolecules at surfaces via biotin-streptavidin interactions. In the final section of the manuscript, we demonstrate compatibility of the polypeptoid brushes with lithographic processes to generate nanoscale patterns offering highresolution chemical contrast and diverse chemical functionalities. We utilize these nanopatterned surfaces to achieve highly selective and localized immobilization of DNA origami nanostructures or streptavidin proteins.