- -On-line 2,6-DHAQ simulated-crossover experiments: Background ferricyanide losses are observed in the absence of 2,6-DHAQ; however, the rate of ferricyanide loss was increased three-fold when 2,6-DHAQ is added. An inverse kinetic isotope effect was observed for ferricyanide losses, but only in the presence of 2,6-DHAQ. There is also evidence that formamide forms chemically within the system, which could be a product of cyanide ligand decomposition. The rate of formamide formation was increased when 2,6-DHAQ was added.