Structure based drug design (SBDD) provides a specific, efficient, and rapid process for lead compound discovery and optimization. Researchers have discovered highly potent and selective molecular glues with SBDD strategies, such as crystallization, in silico modeling, computational docking analysis, rationally designed chemical library constructions, biochemical screening, phenotypic screening, and structure-activity relationship analysis (Table ). • Crystallography • Molecular Docking • Structure activity relationship (SAR) studies • Protein-protein interaction assay of scaffold analogs 68 • E3 ligase dependent activity assay of scaffold analogs • Binding assays • Biochemical methods validating target degradation