The crystal structure of hit 21 in complex with PaFabF C164Q was loaded into SeeSAR. The Inspirator Mode of SeeSAR was used iteratively to grow hit 21 into bigger and bulkier analogues, trying to maximize their interaction with the active site residues, especially with the catalytic histidines. After every round of analogue designing, new analogues were docked or scored-in-place and their binding was analysed using the Docking and Analyzer Modes from SeeSAR, respectively. High scoring compounds that had similar binding modes to the hit compounds and that that formed additional interactions in the binding site were short-listed for purchase.