In the summarised studies 100-103 authors showed that the analysis of mutations' effect can be helpful not only in studying the TLRs' structural dynamics but also in uncovering their mechanism of action, especially in the context of ligand or adaptor protein binding. However, we still have limited knowledge regarding the particular TLRs. Given the fact that many more mutations in TLRs are reported (e.g. in the UniProt or ClinVar 104 databases), more research should be carried out to clarify the effect of those substitutions.