In parallel to their LC-MS 2 profiling in positive (PI) and negative (NI) ionization modes, these samples were screened against three human health-relevant trypanosomatids: L. donovani, T. cruzi, and T. brucei rhodesiense. To illustrate the capacities of the ENPKG approach for incremental sample addition, we also integrated data from three Waltheria indica (Q7966688) samples acquired in the context of a previous project at our lab, including samples obtained using different extraction protocols and profiled in 2014 in PI mode on a different analytical platform . To further showcase the data integration capabilities of our approach, we also integrated publicly available data from 337 methanolic extracts of Korean Pharmacopoeia plants profiled in PI mode on a Q-ToF spectrometer (MSV00008616) It is, for instance, possible to combine the ISDB and SIRIUS/CSI:FingerID structural annotations to retrieve the 33,254 features annotated with the same structure by both approaches (Table , query 1). Using CANOPUS annotations, it is possible to retrieve the samples with the most annotations belonging to a given chemical class, such as aspidosperma-type alkaloids (Table , query 2). This query showed that the extract presenting the highest number of features ( ) annotated with this chemical class was the one from the seeds of Tabernaemontana coffeoides (Q15376858) (Apocynaceae (Q173756)). Based on this information, using a federated query with the SACHEM IDSM endpoint , it is possible to further refine the search to retrieve 3 structural annotations from this extract that contain aspidospermidine (Q15410259) as a substructure (Table , query 3). Taking advantage of the links to WD, it is also possible to enhance the results of SIRIUS/CSI:FingerID structural annotations with their corresponding biological sources and retrieve the ones (17 planar structures at the time of writing) reported in the Tabernaemontana genus (Q310915) (Table , query 4). It is to be noted that this number, and all other results depending on Wikidata federated queries, might evolve if new organism-structure pairs are added (or removed) to (from)