where C b corresponds to the salt concentration in the bulk, P (t) P 0 is the relative probability of opening a pore, A pore is the area of the pore taken from the MD simulation, A vesicle is the experimental mean surface area of the vesicles, and R is the radius of the vesicle. We used the average experimental value (35 nm) for R. We sum the first 10 5 terms of the equation to obtain C(t, N ). The full derivation can be found in SI.