We then evaluated the selectivity of probe ATP-LW towards different biologically relevant interfering species (Fig. and). Other ROS such as H2O2 and HOCl led to no change in fluorescence intensity of ATP-LW (Fig. ). However, ADP (10 mM) induced fluorescence enhancements (Fig. ). Nevertheless, this will not prevent the use of ATP-LW for the detection ATP in cells, since the concentration of ATP is around 1000-fold higher than ADP in cells. We then determined that ATP-LW exhibits good stability over a pH range from 3-11 (Fig. and).