Additionally, as shown in Figure , the offset concentration 𝜎 was approximately -0.5, indicating that the trend is consistent with the experimental result. These results suggest that the differences in the cleavage rate between L † AB and L † AC and the required amount of linker cleavage for B-droplet and C-droplet divisions resulted in 𝜎 ≠ 0. Furthermore, numerical simulations were performed using different parameter values (Supplemental Figures ), producing different offset concentrations. These results suggest that changing DNA sequences could potentially control the offset concentration 𝜎. Note that, when more 𝑐& , the simulation results reproduce the delay in the overall reaction as observed in experiments due to the competition in the RNase H reaction.