The peptide hybrids presented in this work are enzyme-triggered controlled release systems, the structural, chemical and physicochemical properties of which were examined using multiple methodological approaches, including circular dichroism, thioflavin T assay, transmission electron microscopy, atomic force microscopy, scanning electron cryomicroscopy and rheology. The peptides were tested for cytotoxic and pro-proliferative properties on cultured keratinocytes and fibroblasts and, finally, they were applied topically to examine the effects on skin wound healing in mice.