Development of a wetting method. The pores were initially cleaned post fabrication to remove any contaminants by submerging them in piranha solution (3:1 mixture of H2SO4 and 30% (w/w) H2O2 for 5 min., followed by their submersion in deionised water. The pores were then cleaned in an acetone bath and the process repeated with ethanol to remove any residual contaminants. Also, ethanol's low surface tension aids the removal of air bubbles. Next, the pores were plasma treated in air for 30 s per side and then loaded into the measurement cell, where they were wetted with a small amount of ethanol (approximately 25-50 µL). The ethanol was replaced with buffer until the baseline current was stable.