If structural DNA nanotechnology enables to build virtually any architecture on the nanometer scale, the combination with actuation mechanisms could fulfill the dream of a fully synthetic biomolecular machine . Several strategies to control the actuation of DNA nanodevices have been employed. Toehold-mediated strand displacement reaction is a popular approach that relies on user-designed DNA sequences with partial complementarity making it easy to implement. A limiting factor in this strategy is the 2 nd order kinetic of hybridization between DNA sequences that require high concentrations of DNA strands to achieve operational speed in the range of seconds. Alternative approaches for actuation of DNA-based nanodevices include light-responsive molecules , redox stimuli ,pH-dependent secondary structures , actuation by variating the ionic strength of the medium or the temperature and thus controlling the stacking interaction of nucleobases in blunt ends , through the use of external electric or magnetic fields , or biomolecules such as antibodies and enzymes . Most of these strategies proceed stepwise, i.e., the structure switches from one static state to another upon application of the stimulus. Additionally, they operate profoundly differently compared to naturally occurring biomolecular machines in the way they harness the energy required to function. Specifically, the dynamics in synthetic examples either result from a change in the overall environment (e.g., by changing pH, ionic strength), thereby irreversibly driving the system into a new global minimum, or, in the case of light, lifts the system to a different energy landscape. Conversely, natural molecular machines, such as ATPases or motor proteins, couple the catalysis of a chemical reaction, e.g., the hydrolysis of nucleotide triphosphates, to their conformational change: they incessantly consume fuel to drive their motion .