215 However, we found that there is a significant decrease in monodispersity of liposomes around 216 15 μm. To investigate this issue, we conducted simulations using ANSYS Fluent commercial soft- 1.5 nm), to confirm the lamellarity of liposomes due to its easy accessibility and ease of handling 231 . To confirm the unilamellarity of liposomes generated by budding, we performed 232 α-hemolysin insertion assays in the microfluidic device. For the detection of α-hemolysin insertion 233 activity into the membrane, 5 μM Alexa 488 was encapsulated inside the liposomes as a leakage 234 signal. Changes in fluorescent intensity were recorded using confocal microscopy.