Once op�mized the parameters for the fabrica�on of the 3D microscaffolds, the PDMS-induced alignment effect was examined by fabrica�ng an alignment cell as depicted in Figure . Specifically, the glass substrate with the printed PDMS scaffolds and a second substrate were glued together using spacers in between to define the cell thickness. Subsequently, a liquid crystalline material, i.e, E7 mixture, was infiltrated (see details in Experimental Sec�on). To prove the effect of the PDMS scaffolds, the glass substrates used in the study were spin-coated with polyimide (SE-5661, Nissan Chemicals).