Peptide alignment and trajectory analysis was done primarily using the MDAnalysis and NumPy Python packages, together with matplotlib for plotting and VMD version 1.9.3 for molecular visualization and rendering. Tilt angles were computed using the HELANAL algorithm as implemented in MDAnalysis. Because the simulated membranes may undergo a small degree of buckling, thicknesses were computed patch-wise, using the binning implemented in the LiPyphilic Python package .