One of the advantages of the novel soft gold probe developed in this research is to minimize the side effect caused by the topography interference. The soft gold microelectrode could scan the rigged sample surfaces in contact mode (figure and). Therefore, the FcMeOH + generated from HRP-antibody-EGFR complex could be mapped on the tissue surfaces. Although the thickness of the tissue was about 20 μm, the interference caused by probe-sample change and topography was not obvious in figure 3a. If the topography could affect the current signals, the currents over the tissue surface should have been lower. On the contrary, the higher signals from HRP were detected on the tissue surface than the glass surface in figure , suggesting that the effect of topography change could be ignored. As a result, SECM can be used as an imaging method that can uncover certain biomarkers, including IL-6 and EGFR.