In the arthrosis inflammation imaging experiments using NIR-II CLNPs, TPE-BBT successfully generated strong NIR-II CL signals. The highest SBR of TPE-BBT CLNPs in the inflammation area was about 130, and the SBRs remained larger than 10 even after 62 min. The CL imaging quality using TPE-BBT CLNPs outperformed that using TPA-BBT, which demonstrated the importance of the QYs of NIR-II energy acceptors. These results also demonstrated that TPE-BBT showed great potential for CL imaging and sensing.