this compound restores activity of the ALDH2*2 mutant enzyme to near wide-type activity. Indeed, we observed that SNU-182 cells, which express the ALDH2*2 E487K mutant enzyme, treated with Alda-1 for 24 h show a reduction in excess mitochondrial FA load relative to vehicle-treated cells (Figure ). Interestingly, we also found that the basal FA pool was also lowered in HeLa cells by treatment with Alda-1, which establishes that MitoRFAP-2 is sensitive enough to detect endogenous pools of mitochondrial FA in live cells and indicates that ALDH2 activation can be observed when treating cells possessing wild-type ALDH2 with Alda-1 (Figure ).