The difference in coulombic interactions with the stationary phase results in an unacceptable analyte peak shape for reliable quantitation. One charge state is usually fixed (for ionic compounds) by way of eluent additives. Psilocybin has a predicted pKa of . For ionizable or permanently charged complexes like psilocybin, eluent pH must be judiciously prepared for ideal peak shape and resolution. Further, the various methods of preparation or practices may result in some confusion and must therefore be reported clearly to improve reproducibility between labs.