The cells were stimulated with compounds or DMSO for 72 hours at 37°C, 5% CO2, 95% humidity. For detection, we used CellTiter-Glo ® Luminescent Cell Viability Assay (Promega, G7573) an ATP-based detection method of viable cells. This assay applies the properties of a thermostable firefly luciferase to generate a stable "glow-type" luminescent signal which could be measured within minutes when added directly to cell culture.