To further study the e ect of conformer dependent partial charges on calculated AHFEs, we mine FreeSolv for 12 molecules with variable q bond di erences. We run 5-peats of AHFE calculations for these molecules where the only di erence between repeats is the partial charge set employed. We compare the calculated AHFE for each repeat back to experimental hydration free energy data. We find that the FreeSolv molecules also display conformer dependent partial charge variability that a ects our AHFE calculations despite having fewer rotatable bonds than the PLB molecules. The FreeSolv molecules vary in maximal partial charge di erence by up to 0.493 e and in maximal q bond di erence by up to 0.835 e (Table ). We see discrepancies of up to 6.9 ± 0.1 kcal/mol across the five repeats. We find that our AHFE calculations tend to agree with previous freesolv calculations in 9 out of 12 cases (SI Figure ). However, in comparing back to the experimental free energies for the FreeSolv molecules, we find that we are not able to reproduce experimental values.