There are several methods for creating asymmetric liposomes. Large unilamellar vesicles (LUVs) are usually preferred over giant unilamellar vesicles (GUVs) due to their well-defined size and low susceptibility to high curvature or substrate interactions. In contrast, GUVs, though more fragile, have the advantage of being compatible with confocal microscopy imaging and possess a size range of 5 to 100 μm, closely matching the dimensions of eukaryotic cells . This cell-like size makes GUVs valuable as model systems for studying protein-lipid interactions.