Nevertheless, it is relatively difficult and costly to prepare mixed-ligand samples with deuterated ligands. The more convenient way is to directly apply the DIPSHIFT measurement on a mixed-ligand NC with fully protonated ligands (Figure ). We show that the derived interaction energy (𝐸 (mixed), the black line in Figure ) is in good approximation with the result obtained on the samples with selective deuteration: 𝐸 (mixed) ≈ 𝐸 (long + short), despite the signal overlap of long and short ligands. This suggests that the dynamic volume analysis is robust for predicting interaction energy of variable samples without selective deuteration.