In view of the anisotropic flow properties of the LIG-E, we examined its electrochemical characteristics with the aim of correlating these with capillary properties. The permeable electrodes and their unique wicking properties form the building block of the electrochemical platform. For this reason, understanding the effect of wetting on electrochemical performance is essential. We designed and fabricated our electrochemical sensors using a three-electrode configuration, in which voltages are applied and reported relative to a pseudo-reference LIG-E, instead of a standard reference electrode, such as silver/silver chloride. This way, the simplicity and scalability of the fabrication process is greatly improved, while still being sufficiently accurate for almost all electrochemical sensing methodologies. First, we investigated the effect of LIG-E wettability on the effective electroactive areas using a standard redox probe (ferro-/ferricyanide) in two different flow configurations (Fig. ). We interrogated the electrodes using cycling voltammetry (Fig. ) and computed the electroactive area using the Randles-Ševčík equation for diffusion-controlled processes (Fig. ) . The electrochemical porosity factor, η, is defined as the ratio of the electroactive area to the projected area (from the design). In lateral flow, we found that η = 1±0.4 for LIG-E due to poor wettability, but is drastically increased (η = up to 4.0) in oxygen plasma-treated LIG-E (Fig. ). On the other hand, and echoing the moving front experiment, we observed high porosity factors in the vertical flow format for both LIG-E and plasma-treated LIG-E, showing η = 5.7±0.2 and 6.3±0.3, respectively. These findings suggest that the top surface is both hydrophilic and electroactive, although it is not fully accessible in lateral flow configurations. We next evaluated the electron transfer rates of the LIG-E using impedance spectroscopy (Fig. ) .