For system 1, barstar was pulled away while barnase was kept fixed, for system 2 the two monomers of the HdeA dimer are identical, so one of the monomers was kept fixed and the other one was pulled. For systems 3 and 4, the peptide or ligand was pulled away while the protein was kept fixed. For system 5, the subunit A was pulled away from the rest of the protein. In Figure the dissociated parts are shown in orange.