Final conditions: See the procedure for Figure Preparation of Pd solutions for calibration curve See the procedure for Figure Preparation of 900 µM TFP/100 mM NaBH4/375 mM NaOH in 6% DMSO/44% EtOH/water See the procedure for Figure Assay 3) Measure fluorescence with a green filter. If the standard deviation is too large, pipette again onto a different plate.