Structural differences accompanying phosphorylation of G159D, in both magnitude and sign, can be observed in respect of the interaction of the N-helix of cTnC and the beginning of the H1 helix of the NcTnI (Supplement Table , Supplement Figure H,I). The key phosphorylation-dependent interactions between cTnI Arg19, Arg21, Ser22, Ser23 and Arg26 with cTnC Lys33 and Lys39 are similar to wild-type.