The availability of nutrients is needed for the stability of anaerobic digester for biogas production which is operated with energy crops, animal excreta, crop residues, organic fraction of municipal solid wastes or any other type of organic waste , and have been studied in detail . As compared to the anaerobic digestion, the substrate for the ex-situ biomethanation in BTF is simple, i.e. H 2 and CO 2 , hence nutrients supplementation is more essential. However, to the best of our knowledge, analytical investigation of nutrient consumption and the analysis of nutrient dosing and supply methods have not been carried out for BTF, specifically for the long-term continuous operation of the reactor. In this study, an optimization of the nutrients dosing and supply method was studied for stable operation of ex-situ biomethanation in a BTF. Essential nutrients were identified along with their minimum concentration levels, followed by a systematic approach to find the effective method of supplying these nutrients. The dosing method relates to the frequency of supplementation of the nutrients in the nutrient media sump and the supply method relates to the method of trickling the nutrient media over the biofilm in the BTF. After the optimization of the nutrient dosing and supply methods, the maximization of methane production was investigated.