Deconvolution was not conducted on the peak between 500 and 600 nm, as this region was attributed to the SPR peak of the AuNPs. The LN-MIE-Gans model fitting taken from literature of 20 nm AuNPs evidences this (Figure ), as it can predict the radius of the AuNPs based on the fit of the model to the spectra (Figure ). For the spectrum of 20 nm cit-AuNPs, the radius is predicted to be 10 nm with a resultant predicted diameter of 20 nm. The predicted diameter correlates with those calculated previously from the TEM at 19.15±2.12 nm and DLS at 19.57±1.03 nm (cit-AuNPs), therefore it can be assumed that the only region of interest for deconvolution in these examples is within the Soret band region.