Both the low-angle ADF-STEM image and the corresponding diffraction patterns show distinct differences to the features observed in C31H64 crystals (Figure , Figure ). First, no grain structure is observed in the low-angle ADF-STEM image of C30H61OH. Overall, the crystal exhibits a blocky structure as seen in TEM (Figure ), but grain structure is not observed within these blocks in either TEM or SED. Where there are variations in contrast, inspection of diffraction patterns indicate that these arise from mass-thickness contrast (Figures ).