Herein we present an accessible and facile approach to improving the interaction of cells with an organic semiconducting (OSC) polymer film through UV-Ozone treatment. Variation of UVO exposure time was carried out to analyse the effect on pDPP3T films, which revealed an increase in the surface energy in combination with reduced surface roughness through contact angle and AFM measurements respectively. These properties culminated in an enhancement in Schwann cell growth on the surface with exposure times as low as t = 30 s. In addition to enhanced biocompatibility, retention of the optoelectronic properties in conjugated polymers is central to their application in bioelectronics. The effects of UVO exposure were monitored using UV-Vis absorption and field-effect measurements. These revealed that while the ICT band decreased relative to the π-π* band and the hole mobility declined with increasing exposure time, both changes were negligible for t ≤ 30 seconds.