Some kinase activities measured in the profiling dataset are rather unexpected from a sequence-based similarity point of view. For the EGFR-Erlotinib example, we use the KinMap server to plot the profiling-based and KiSSim-based ranked kinases onto the kinome tree by Manning et al. . For example, we highlight kinases with measured activities against Erlotinib as well as the 50 most similar kinases to EGFR as detected by KiSSim. All kinases that are part of the KiSSim dataset are shown as well to define which data points are available for similarity predictions.