As a natural continuation to the introduction of ROMANCE in our previous article, we herein present ROMANCE v2. This new version of the software has been developed to take into account new operational scenarios such as non-constant electric fields, the use of more than one reference compound, and the influence of the conversion or the ionization regime on the peak area. These and other relevant fundamental considerations are described in detail in the provided theoretical framework before illustrating their utility and performance using a panel of reference compounds used for validation and then, a metabolomics study conducted on a small set of cell culture samples.