To further support the SAXS fits and interpretation, SPA was used to obtain and group together multiple cryo-TEM projection images of 0%, 50% and 100% PBd22-PEO14 hybrid vesicles to give a high-resolution image of sections of membrane for each sample as shown in Figure . A fast Fourier transform (FFT) of 10 cryo-ET images resulted in a scattering pattern similar to the SAXS scattering curves (see SI Section 6). These FFT patterns were also fitted with either the 3-Gaussian model (Eq 1) for thin membranes or the 4-Gaussian model (Eq 2) for thick membranes to give an electron intensity profile of the membrane that can be compared to the electron density and intensity profiles from SAXS and cryo-TEM techniques.