The gpGVHSV from 89 amino acid sequences of different VHSV isolates from diverse world-wide sources were aligned by Clone Manager vs9. The 67 EFEDIN and 302 LNHLIT amino acid sequences around the R4-linker and the TrD showed only one (I71T) or three (N203G) mutations, respectively (Supporting Materials / 89VHSValignement.docx).The gpGVHSV amino acid sequences were aligned to gpGVSV and other rhabdoviruses, maximizing Cystein and the most important amino acid sequences implicated in the gpGVSV fusion transitions (Table ) . The crystallographic gpGVSV ectodomain RCBS-PDB models (5I2S for pre-fusion and 5I2M for post-fusion), were employed as templates to Swiss model the gpGVHSV sequence (VHSV07.71 of 507 amino acids from UniProtKB P27662, GLYCO_VHSV0) (). Previously to the modeling, the corresponding homotrimer structures were generated from the original pdb files containing crystallographic information (PyMol/A/Generate/ symmetry mates/within 4 Å). The Swiss-modeling was preferred to alphafold because of similar monomer and best pre-fusion homotrimer structure predictions.