Afterwards, it was ramped to 100% B over 2 min and held for an additional minute at this concentration before returning to 95% A. For extended LCMS runs, separations were conducted on an Agilent 1290 Infinity II LC System using an Agilent Infinity Lab PoroShell 120 EC-C18 column (30 °C, 2.7 μm, 2.1 × 50 mm). LC conditions were set at 95% water with 0.1% formic acid (A) ramped linearly over 15.1 mins to 100% acetonitrile with 0.1% formic acid (B) and held until 15.3 min. At 15.4 min the gradient was switched back to 95% A and allowed to re-equilibrate until 18.0 min. Injection volume for all samples was 4 uL. Preparative HPLC was performed using an Agilent 1260 Infinity II LC System equipped with a Phenomenex C18 column (PhenylHexyl, 30 °C, 5 μm, 75 x 30 mm) using the following method: Solvent A: water + 0.05% TFA; Solvent B: Acetonitrile; flow rate: 30.00 mL/min. LC conditions were set at 95% A ramped linearly over 26 min to 100% B and held until 28 min at 100% B. At 30 min the gradient was switched back to 95% A. Final compounds were determined to be ≥95% purity by analytical LCMS.