To further validate our IL models, we took advantage of our building block approach for constructing ILs and extended our tests to a larger sub-selection of headgroups, linkers, and tails in 15 × 15 nm 2 IL:DSPC (50:50 mol%) bilayer patches. From these simulations, we calculated the area per lipid (APL) and the hydrophobic thickness, specifically the distances between the two leaflets excluding the polar headgroups. See Figure for the head/linkers and tails under study along with the APL and thickness values. The APL was only calculated for the charged species that maintained the lamellar topology. A trend was observed across the lipid tails, with the DU tail (CDDC CDDC) having the highest APL. The fully saturated tails DL and DS consistently showed lower and similar APL across the lipid types (see Figure for more details). This trend aligns with observations in common phospholipids, where polyunsaturated tails exhibit higher APL compared to saturated ones . The choice between saturated and unsaturated tails for LNP usage is a topic of discussion in the literature; unsaturated tails, despite being prone to oxidation and presenting an instability issue, have demonstrated a significant improvement in LNP transfection efficacy .