In the present study, we have successfully realized transparent bactericidal coatings via hand spray-based approach under ambient conditions for 15 min. The as-generated fivemetal oxide coatings exhibit transmittance values of 99.74% with a strong adhesion on screen protectors of smartphones. The high bactericidal activity against E. coli (with viability at 3.14%) has been observed. The films exhibit strong durability and retain bactericidal activities against the finger swiping and wet cleaning for at least a month. The incorporation of Cu 2+ is responsible for the major activities, while the presence of Ag + , Zn 2+ are crucial to achieve nearly 100% bactericidal activity, as compared to only Cu 2+ doping cases. We further demonstrated the success of realizing this bactericidal coating on other typical hand-touched surfaces (e.g. stainless steel railings, rubber hand rails, and plastic switches) commonly seen in public areas.