This experiment consisted of three separate templates: a nucleic acid sample, RNase-free Water (Negative Control or No Template Control [NTC]), and a sample SARS-CoV-2 (Positive Control or PC). The initial step consisted of preparation of a master mix that consists of 2 μL mixture of enzymes (enzyme mix or EM), 4 μL 5x buffer mix (reaction mix or RM), 4 μL mixture of primers and probes (oligo mix or OM), and 7 μL distilled, sterile, RNase-free water. The measurements were for one round of the PCR test. For the experiment, each template was tested four times for accuracy and precision, meaning that a total of 12 times (3 template x 4 repetition + 3 more times to ensure sufficiency of the components) of each component was used. The vortex and the centrifuge were utilized to mix these components. Then, the master mix was split into 12 separate wells in a 96-well plate, and each of the four wells were mixed with 3 μL of the test sample, NTC, and PC each. After this process, the well plate was placed into a small box before moving to the room with the PCR machine to avoid any possible contamination or exposure to strong lights, as failures in sample collection and storing methodology could create false-positive or false-negative results .