This approach would allow microtomy to be performed on the entire stack. Alternatively, sapphire disks can be employed as bottom and top film (like CryoCapCell) due to their high thermal conductivity. However, these would have to be removed prior to microtomy. A 50 µm-thick inert gold (Au) ring separates the top and bottom discs. An emulsion is placed in the middle of the Au ring for monitoring. Furthermore, a newly designed sample holder that can be mixed in a microscopy stage allows for the placement of multiple carriers (left). This is advantageous as monitoring oxidation can be performed in a parallel/circular fashion, considering that oxidation in food emulsions is a long-term process. Moreover, the carrier can be used for both upright and inverted microscopy since both enclosing membranes are transparent. (c) Design of the disc used in HPF carrier to identify oxidation events during cryo-microtomy. The zoomed-in region of the disc shows a marker that helps to identify the region of interest during cryo-microtomy. Cryomicrotomy is necessary to prepare thin samples for cryo-TEM imaging. In this cryo-CLEM workflow, a specific location of oxidation events/regions can be roughly identified from a sign/pattern of the disc in the HPF carrier and further trimmed to obtain cryo-samples containing the regions of interest. The thin section of a sample is then deposited in a finder grid and further imaged by cryo-FM, after which cryo-TEM is conducted at the same location.