Eurofins kinase enzymatic radiometric assays were executed at the Km value for ATP in duplicate using a single concentration (1 μM) to generate PoC values for TTBK1 (accession number: NM_032538.1, 1-479 recombinant construct) and TTBK2 (accession number: BC071556.1, 1-333 recombinant construct). TTBK1/2 was incubated with the substrate (2 mg/mL casein) and tracer (γ-33P ATP) in 8 mM MOPS (pH 7.0), 0.2 mM EDTA, and 10 mM magnesium acetate. The reaction was started by adding the Mg/ATP mix. After incubating at room temperature for 40 min, the reaction was stopped by adding phosphoric acid (0.5%). The reaction mixture was then spotted onto a filter, washed four times with phosphoric acid (4 min in 0.425%) and once with methanol, and dried prior to scintillation counting. Staurosporine was used as a positive control compound for the assay. Further information for these two kinase assays is available on the Eurofins website: .