HABA Biotin Binding Assay with MinE07-Biotin, MinE07UA-Biotin, MinE07UA'-Biotin, MinE07UB-Biotin, MinE07UC-Biotin and MinE07UC'-Biotin Aptamers. Streptavidin-AP at a 1:1000 dilution in binding buffer was prepared, 20 µL was added to each well and it was incubated for 30 minutes at room temperature shaking at 450 rpm with 20 µl of Tropix CDP-Star ready to use substrate. The plate was then read using the chemiluminescence protocol on the Victor X4 plate reader to check it was giving a signal. 20 µL of HABA (5 µM) was added to the wells and it was incubated for 30 minutes at room temperature shaking at 450rpm. MinE07UA-Biotin, MinE07UA'-Biotin, MinE07UB-Biotin, MinE07UC-Biotin and MinE07UC'-Biotin were added to relevant wells and incubated for 30 minutes at room temperature shaking at 450rpm. The plate was then read using the chemiluminescence protocol on the Victor X4 plate reader.