Figure shows that with a 40× objective lens and a g-Odot concentration of 7.5´10 7 particles mL - 1 , there is low positive correlation between the g-Odots and the LysoTracker; however, this value may be artificially closer to zero due to noise in the image from the dim signal of the g-Odots. When imaged with a 63× objective lens and a g-Odot concentration of 1.5´10 9 particles mL -1 (Figure ), the correlation nearly doubles, with a Pearson correlation coefficient of 0.617. Previous work by Adachi and co-workers in 2019 and Hudson and co-workers in 2022 showcased g-Odots with much larger average diameters for cellular imaging in HEK293 cells such that cellular uptake likely occurred through micropinocytosis whereas for particles much smaller than 200 nm, macropinocytosis may not be the most likely uptake pathway. Their large size may have also been the reason the particles were traceable over many instances of passaging, with some g-Odots still observed within cells after 21 days; larger particles typically accumulate within lysosomes less efficiently than smaller ones, which could explain why these particles are located more frequently in the lysosomes after just 2 hours of incubation. Additionally, on a cell-by-cell basis it is apparent that there is less uptake of the g-Odots than the small molecule LysoTracker dye. This is evidenced by comparing the signal-to-noise (SNR) and signal-to-background (SBR) ratios (Figure ). The LysoTracker exhibits a SNR of 689.4 ± 133.3 and a SBR of 960.2 ± 185.6, while in contrast, the g-Odots exhibit a SNR of 8.04 ± 6.7 and a SBR of 1.06 ± 0.88. Compared to previous literature reports, these values are much lower and support the notion that uptake is significantly reduced for these smaller g-Odots. As well, nanoparticles with coronas made of poly(ethylene glycol) have been shown to have increased circulation time in circulatory systems as a result of increased hydrophilicity, which could explain the limited uptake by g-Odots in immortalized cell lines. As such, we believe that in future studies these nanoparticles would be well suited to applications such as vasculature imaging, where increased circulation time is an asset and deeper light penetration is required, or assays in which cellular uptake is not required.