Prediction of host-guest binding in MOCs is non-trivial to do reliably via computation, and curation of experimental data to validate these predictions or feed a data-led approach remains a bottleneck. The most common approach to study and quantify host-guest binding in supramolecular assemblies is to study the change of one species' physical property, such as a chemical resonance in NMR spectroscopy, or the absorption band in UV-Vis spectroscopy. Typically host-guest binding studies using NMR are time consuming and expensive, requiring a large amount of material and deuterated solvents, especially for full titrations. Our aim here was therefore to develop a high-throughput host:guest assay in a plate-based format for MOCs. The ability to carry out many single-point NMR binding studies at a known host-guest equivalence in parallel, performed in an automated way, allows for the rapid identification of strong binding guests, the elucidation of trends in binding behavior, and the potential identification of promising molecular separations.