Mass spectrometry-based proteomic analysis revealed that exosomes were enriched with neurotrophic factors such as BDNF, GDNF, and VEGF, as well as anti-inflammatory miRNAs like miR-21, miR-124, and miR-146a. These molecules are critical for modulating inflammation and promoting neuronal survival. Flow cytometry further verified the expression of exosome surface markers, adding quantitative validation to the characterization efforts.