This approach was also used to investigate the reorientation of phospholipids at the lipid/water interface via HR relaxometry of 31 P, highlighting how temperature and cholesterol impact lipid dynamics. This study showed that cholesterol reduced the energy barrier for phospholipid motion, thereby contributing to membrane fluidity and stability. Supporting this, Sivanandam et al. applied C high-resolution field-cycling NMR to assess the "wobble" of PC in vesicles,