Continuous, long-term monitoring was achieved through a network of sensors and analyzers that interfaced with a supervisory control and data acquisition system (SCADA) and were complemented by alternate-day elemental analysis of the solids and twice daily aqueous and total suspended solids (TSS) analyses during weekdays. These long-term monitoring data were supplemented with batch kinetic assays to characterize nutrient and carbohydrate storage dynamics in the mix tank and PBRs. Ultimately, a deeper understanding of the EcoRecover system will support further system optimization and control to advance the sustainability of microalgal wastewater treatment technologies and biological nutrient recovery.