To increase accuracy for hotspot prediction we introduced the Dynamic-FMOPhore (Dy-FMOPhore) approach, wherein the probes were allowed to scan the binding site of the target in a complete dynamic solvated environment using supervised molecular dynamics (SuMD). We performed Dy-FMOPhore on systems that reported a recall lower than 0.9 A good example that demonstrates the success of Dy-FMOPhore is the PL-pro, (Figure .). PL-pro binding site is known to be formed by a P-loop (Tyr-A-268, and Gln-A-269) that is highly flexible, we previously observed that the binding site is formed upon the induced-fit binding event that happened between the binding site residues and the ligands. By Scanning of the PL-pro apo-structure (PB-ID: 6W9C), static binding site using the probes library, it was found that FP-score categorizes Asp-A-164, and Arg-A-166 as the key Anchor-category residues. While Lys-A157, Glu-A-167, and Asp-A-302, were categorized as Transient-category. Accessible-category residues were Pro-A-248, Tyr-A-264, Tyr-273, and Thr-A-301. The rest of the binding site was categorized as Nonhotspots.