At high resolu on (Figure , le ), the spectra are dis nct for each component and consistent with the corresponding spontaneous Raman spectra, except for the rela ve intensi es of the spectral featuresthe SRS intensi es towards the center of the spectral range are higher while those at the edges are diminished, when compared to the spontaneous Raman spectra. This difference is a consequence of the changing pulse overlap employed during spectral focusing (see Figure in the Suppor ng Informa on for a pictorial explana on), causing a Gaussian-like intensity profile mul plying the spectra. The lowresolu on spectra (Figure , right) are dis nct for RTV, PVPVA and buffer, but cr-RTV and am-RTV are not clearly dis nguishable. For this reason, correla ve SFG together with the high-resolu on spectra was addi onally recorded at the end of each experiment to detect any RTV crystalliza on.