Gel filtration by Sephadex G-50 was investigated for purification of liposomes form the free peptide and solvents. Sephadex G-50 resin was chosen allowing separation of molecules ranging between 1.5 kDa -30 kDa. Teriparatide having a MW (4.1 kDa) close to the lower limit may access a large fraction of the volume provided by the gel, which should result in separation from the liposomes. Compared to frequently used PD-10 desalting column which uses Sephadex G-25. These resins have a separation range of 800 -5000 Da. The peptide would be expected to elute rapidly as it's MW is close to the upper limit of the separation range and therefore excluded from accessing most of the volume. The prepared column successfully separates liposomes from any free peptide as can be observed from Figure .1a where the dark blue trace represents the elution of liposomes between fraction 4-6. The same fractions are devoid of any free peptide (light blue trace). Indeed, peptide elution was only observed from fraction 8 and continued through to fraction 14. The experiment further allowed to determine the volume required to reconstitute the column determined by complete elution of the peptide and was determined to be 8 mL.