The wavelength-dependent fluorescence signal-to-noise ratio simulation was written in Python (version 3.8, packages used: numpy 2.0.1, scipy 1.14.1, matplotlib 3.9.2, pandas 2.2.2, colorcet 3.1.0). The code used for the simulations is available on GitHub at . Extended methods section 1 (Supporting Information) explains the workflow and the fundamental equations of the simulation. In Tables , and S3 all parameters that influence the simulation are listed and referenced.