Fluorescence anisotropy of mAG reduced in a chain length-dependent manner, indicating the peptide lengthdependent assembly (Fig. ). Considering that NES-YK13-mAG did not form droplets, we supposed that the interaction between Nck1(1-258) and N-WASP contributed to droplet formation. To investigate the actin polymerization, we performed phalloidin staining after cell fixation. The results revealed that the actin comet tails were extended from the artificial droplets (Fig. ).