Nanoparticle-based delivery systems have emerged as an attractive approach for the safe and effective delivery of a diverse range of drugs through altered organ biodistribution and controlled drug release. Upon administration to a biological system, colloidal nanoparticles will encounter and interact with biomacromolecules and cells in their immediate environment over time, following which spontaneous surface-adsorption of biomolecules including proteins occurs. The protein corona-has a fundamental impact on the chemical and biological identity of nanoparticles, and their subsequent biological fate . Formation of the protein corona is a process governed by nanoparticle physical and chemical properties, and the composition of biological fluids to which nanoparticles are introduced. A variety of nanoparticle and biological fluid composition parameters are known to influence this process. Knowledge of the protein corona composition and its impact on nanoparticle physical and chemical characteristics is crucial for understanding protein corona effects on biological fate of nanomedicine, which can inform the design of novel nanoparticle prototypes with pre-defined target organs, release rates, immune responses, and circulation times . These observations have been attributed to the formation of the surfaceadsorbed nanoparticle protein corona. More recently, the non-specific adsorption of other biomolecules (e.g., DNA and sugars) has been considered to impact the composition of the protein corona and lead to observed effects on nanoparticle biological fate . Though associations between nanoparticle surface charge, morphology and the composition of the protein corona have been reported in numerous studies , a direct comprehensive link between nanoparticle physicochemical properties, composition of the protein corona and biological fate are yet to be established.