To verify that 258HP-pso-TFO-PEG complex formation was through TFO-HP Hoogsteen interactions, we also performed AGE analysis of the pso-TFO-PEG5K complex at pH 8.0, with the expectation that increasing the pH during electrophoresis would lead to dissociation of the pegylated TFO. As expected, the mobility of the nanostructure at pH 8.0 returned to that observed for the TFO-free 258HP nanostructure (Fig ).