MeODPA-DiKTa g-Odots in water are shown in Figures and. The MeODPA-DiKTa g-Odots show a deeper red emission at l PL of 663 nm, compared to the 5 wt% doped film of MeODPA-DiKTa in mCP (l PL = 655 nm), while the PhDPA-DiKTa g-Odots emit at l PL of 618 nm, similar to that in the 5 wt% doped film in mCP (l PL = 617 nm). Both g-Odots exhibit TADF, with a t d of 203.9 µs for PhDPA-DiKTa and 131.6 µs for MeODPA-DiKTa (Figure ). The F PL values of the g-Odots has been slightly enhanced (Φ PL = 77% for PhDPA-DiKTa g-Odots; Φ PL = 38% for MeODPA-DiKTa g-Odots) for both compounds compared to the corresponding doped films, a similar phenomenon observed in our previous report. Due to the higher F PL of the PhDPA-DiKTa g-Odots, their potential as cellular imaging reagents was evaluated first. HeLa cells were cultured inside of an 8-well plate, and the cells were incubated with g-Odots suspended in serum-free Dulbecco's Modified Eagle Medium (DMEM) and LysoTracker TM Blue DND-22 (100 nM) to determine whether the g-Odots localize within lysosomes.