This result seems to imply that the total chiral signal is zero. However, in reality, the symmetric and asymmetric signals are shifted in frequency relative to one another due to both vibrational coupling and heterogeneous local environments. The asymmetric component is typically assumed to have the higher frequency because the average intramolecular coupling is negative. Hence, the symmetric and asymmetric responses do not perfectly cancel, and instead they produce a characteristic two-peak line shape (Figure ). This two-peak line shape can be "up-down" or "down-up". When the symmetric stretch response is positive and the asymmetric stretch is negative, the line shape will be "up-down" (Figure ), and vice versa for a "down-up" line shape.