Herein, we apply our Hps40 affinity platform to identify proteins that are destabilized after cellular exposure to chloroacetanilide herbicides. We profile the effects of acetochlor, alachlor, and propachlor on the HEK293T proteome and discovered distinct profiles of destabilized proteins for each condition, with most protein targets harboring reactive cysteines. Propachlor in particular targets Parksinsons' associated proteins GAPDH and PARK7, decreasing their cellular activity. Hsp40 affinity profiling is a simple method for rapidly identifying proteins that are destabilized by cellular exposure.