In the following, we establish our procedure to predict the interphase thickness from modulus maps alone and discuss the minimum thickness required to apply our approach. We estimate the interphase thickness by fitting a sigmoid function to the difference between master curve and normalized modulus profile, i.e., (E/EFF-EMC). To exclude the modulus increase near x = 0, we use the range where (E/EFF-EMC) < 1.1 and xdirect < x for the sigmoid fitting. To probe the robustness of fitting, we conducted 500 bootstrap iterations for each fit. For a quantitative discussion of the lower limit of interphase resolution, we defined E0 as the value of the fitted sigmoidal function at x = 0. The modulus ratio between I and M layer is 1.8 in our artificial interphase model, which ideally yields E/EFF-EMC = 0.8 at x = 0. Thus, we expect that a successful sigmoidal fitting will return E0 = 0.8. the case where (E/EFF) collapses with the master curve and (E/EFF-EMC) does not show the evidence of an interphase (λphase /aFF) < 2, E0 becomes negative which indicate that the sigmoid fitting fails.