To increase the inter-and intramolecular crosslinking of the KAP5-1 segment used in our F-layer molecular model, the Crosslink Polymers module was used . Cysteine residues in proximity to each other were allowed to form disulfide bonds, and the resulting H 2 gas molecules were deleted immediately after a disulfide bond was created. These crosslinking simulations were performed by searching for candidate -S-H bonds with a maximum search radius of 5 Å. A maximum number of five disulfide bonds were allowed per iteration, followed by 50 ps of MD relaxation at 300K. Each crosslinking simulation terminated after 10 unsuccessful crosslinking attempts. An attempt is defined, in the context of the Polymer Crosslinking module, as a search/bond formation step followed by an MD relaxation in the NPT ensemble. For each unsuccessful attempt, the 50 ps MD relaxation step is still performed before progressing to the next iteration.