Based on the minimal QM region containing the base pair G7-C14, we performed sbPCVA analogous to the saaPCVA scheme. A single point calculation is performed for each of the remaining 18 bases in which the charges of the corresponding base are varied. Afterwards, sensitivities are calculated for each base. In contrast to protein systems, here we will waive the calculation of indicators because they do not add value to the results since we deal with a linear distance behavior due to the structural properties of a DNA helix, and will thus use the sensitivity ranking directly for the construction of QM regions.