• 10 µL Alexa405 SiO2 NP 1% w/v% To quantify the encapsulation efficiency, post-polymerization aliquots were diluted 1:10 in PBS, and run through PD 10 desalting columns (Cytvia; gravity protocol) to remove the unencapsulated molecules. The second fraction (7 mL, per supplier's instructions) was recovered as well. Alexa405 SiO2 NP were instead recovered by centrifuging a 1 mL-aliquot of vesicles (500 x g, 3 min, in an Eppendorf microcentrifuge), and recovering the supernatant.