Human tyrosinase (hsTYR, EC 1.14.18.1) is a type-3 copper-containing metalloenzyme characterized by a coupled binuclear active site that performs both the ortho-hydroxylation of monophenols and the oxidation of catechols. Especially, hsTYR carries out the hydroxylation of L-tyrosine to L-DOPA and the subsequent oxidation of L-DOPA to dopaquinone, thereby initiating the spontaneous synthesis of melanin pigments in specialized skin cells, i.e. the melanocytes. Melanin plays a crucial role in cell protection against UV radiations and free radicals.