Reagents should be under argon when in use and stored properly using parafilm. Diluent and activator should be stored in a dessicator cabinet supplied with Drierite (Fisher Scientific, 07-578-3A). Triphosphorylating and diphosphorylating reagents should be stored in a jar with dessicant (Drierite) at 4C. All tubing and seals on the MerMade 12 should be checked frequently to ensure no leaks exist within the system. Fresh diphosphorylating reagents should be used when executing a new synthesis and taken off before the machine will be dormant for several days to avoid clogging of the lines. Bottles should be baked for a minimum of 1 hour at 150C and cooled prior to use for reagents. Prior to any addition to a reagent prime each syringe with argon twice to ensure a dry environment.