Capture strips were cut 17 mm from the bottom of the test strip, removing the absorbent pad. As depicted in the Supplementary Information (Figure ), a strip of adhesive tape (10 × 30 mm) was then used to fix and assemble the AmpliFold test, such that 2 mm from the top of the capture strip interfaced the detection strip, 2 mm from the bottom, with aligned, membrane-to-membrane contact.