The instrument has a maximum output of 50 mW. The prin ng GWL files for 3D structure fabrica on were generated from STL files of the desired geometries with the help of the Describe so ware (Nanoscribe). Slicing and hatching were set both to 300 nm. Prin ng was performed with laser powers in the range of 10 -30 mW and scanning speeds between 200 -20000 µm s -1 depending on the desired structure. Silanized cover slides were a ached by tape onto a commercial sample holder (Nanoscribe) for oil immersion mode. Immersion oil was added on the unfunc onalized slide surface, and the ink on the func onalized one to ensure good adhesion of the 3D printed microstructures. To maintain the environmental condi ons of the ink as reproducible as possible, the ink was loaded into a PDMS mold and covered with a circular coverslip during prin ng. A er prin ng, the PDMS mold was removed and uncured ink was removed. Different developing procedures were screened, including developing 10 min in hexane, 5 min in 1:1 acetone/isopropanol mixture and washing with water and soap as previously reported, followed by drying. Employing water and soap yielded similar results to using organic solvents. 3D prin ng and the en re sample prepara on and development were performed under yellow light condi ons.