Commercially available fragment hits binding in or close to the malonyl-CoA binding site or the fatty acid binding site (Table ) were subjected to binding constant determination using bio-layer interferometry (BLI) as described earlier. These were all compounds from the DSi-Poised library except of 33 which was unavailable for re-purchase (Table ). However, reliable binding data could not be obtained for any of the hits as no or only low signals were obtained in the BLI assay (Table ).