The observed Bragg spot intensity variations can be understood as arising from small variations in the local orientation of the crystal within these rectangular regions or 'grains.' Through comparison with kinematical simulations of mis-tilted C31H64 crystals (Figure ), the spatially isolated patterns (Figure ) were assigned to orientations <1° from the [100] zone axis. The misorientation analysis revealed two angular variations, one away from the [100] axis and a further rotation about this axis, though with no rotation of the pattern on the detector itself. The variation in the second rotation about [100] follows a consistent counterclockwise rotation in the diffraction data when moving from left to right in the image (Figure ), suggesting a degree of coherence likely emerging during crystal growth on or against adjacent grains within the larger wax crystal plate.