Compactness (Rg/Rh) and flexibility (Porod exponent) are two parameters of interest in the realm of SCNP design. Figure illustrates kernel density estimation (KDE) and principal component analysis (PCA) that were completed for the dataset to observe differences in flexibility between subgroups. Compared to copolymer backbones, the conjugation of the EPO peptide on average seemed to shift behavior towards that of proteins. These EPO-SCNPs tended towards increased Porod exponent compared to that of copolymer backbones, showing greater similarity to the behavior of the PDB proteins. The other subgroups of synthesized SCNPs (PEG and WDCL) displayed more of a disparity with PDB proteins compared to EPO-SCNPs (Figure ). Rg/Rh seems to have greater complexity as the two groups of polymer-peptide conjugates, the EPO-SCNPs and WDCL-SCNPs, have greater overlap with compactness of PDB proteins than copolymer backbones and PEG-SCNPs (Figure ). This is also reflected in the shift in median Rg/Rh and Porod exponent, respectively, between the groups: Backbone (1.13, 1.8), PEG-SCNPs (0.98, 1.8), WDCL SCNPs (0.91, 2.1), EPO-SCNPs (0.88, 2.8), and PDB proteins (0.85, 3.6).