Escherichia coli (E. coli, NTCT 10418) and Staphylococcus aureus (S. aureus, ATCC 29213, purchased at DMSZ as DSM 2569, wound isolate, Methicillin sensitive S. aureus) glycerol stocks stored at -80°C were streaked onto LB (Lysogeny broth) agar (Carl Roth, Karlsruhe, Germany) and incubated at 37 °C until visible colonies were grown. Overnight cultures were prepared by inoculating 5 mL of LB (Luria/Miller: 10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.0 +/-0.2) with a single colony and incubated at 200 rpm and 37°C (incubator MaxQ6000, Fisher Scientific, Hampton, NH, USA) for 16 h to 18 h. Overnight cultures were set to an OD 600 of 0.5 in LB in semi-micro cuvettes (1.6 mL Rotilabo single-use, Carl Roth) and diluted 10-fold in LB. 1 mL of the cultures were then centrifuged at 10.000 rpm for 10 minutes at RT and the pellet was washed 2 times with PBS (phosphate-buffered saline, without calcium or magnesium; Lonza Walkersville, MD USA) and finally resuspended in 1 mL PBS. A further 100-fold dilution of the bacteria suspension in PBS resulted in a starting bacterial inoculum of 5.9 +/-0.2 log 10 CFU/mL for S. aureus and 5.3 +/-0.1 log 10 CFU/mL for E. coli.