The conjugated OA-Gly-OH, OA-Thr-OH, and OA-Phe-OH were synthesized through the reaction between n-octanoyl chloride and amino acids respectively. The resulting products are confirmed via NMR spectroscopy.The selection of these substrates was guided by their varying side chain structures: glycine, with no side chain, threonine, with a branched side chain, and phenylalanine, with an aromatic side chain. These structural differences result in a progressive decrease in hydrophilicity in the order of glycine > threonine > phenylalanine. This systematic variation in side chain properties provides a foundation for studying how an addition of amide bond and different side chains affect the self-assembly behavior and CAC of the conjugates.