We aimed to create a scaffold biologically relevant to the liver ECM in terms of microstructures and biochemical composition. Literature suggests that the native liver ECM is a fibrous network, with estimated ECM fiber diameters ranging from 100 to 500 nm . Thus, we used electrospinning to generate nanofibers similar in thickness to those in the native ECM. Electrospinning transforms a polymer solution into nano-/microfibers by pumping the solution through a syringe needle at a high voltage, forming a Taylor cone . By varying parameters such as voltage, needle diameter, flow rate, and distance to the collecting platform, the fiber size, orientation, porosity, and layer thickness can be tuned . Polycaprolactone (PCL), a biocompatible polymer, was used as the base material to make the fibers . Meanwhile, cut pieces of porcine liver, which have proven to be similar to the human liver for various biological applications , were decellularized to collect the native decellularized ECM (dECM), which was then ground, digested by pepsin and lyophilized. This dECM was added to the PCL solution before electrospinning so that the fibers would contain the native ECM components. Ideally, a larger ratio of the native dECM to PCL would be more biorelevant. However, we observed that if the native dECM exceeded the amount of PCL (weight), the dECM started to precipitate and form layers while in the electrospinning solution. Therefore, we made the solution with 10% PCL (w/v) and 10% (w/v) dECM, which remained homogenous for electrospinning.