One of the limitations of the Martini 2 force field was the inability to recreate the experimentally observed phase separation 134-137 that occurs in three-component systems comprising a low Tm lipid (such as DOPC), high Tm lipid (such as DPPC), and cholesterol . In order to utilize Martini to investigate phase separating phenomena, one either had to use the less physiologically-relevant lipid DliPC (18:2/18:2, 1,2-Linoleoyl-sn-glycero-3-phosphocholine) or modify the Martini parameters . Thus, the degree to which the Martini 3 lipid can reproduce phase coexistence in these three-component systems is of great interest.