Biology uses pumps to maintain concentration gradients, 1-2 regulate blood pressure, 3 cell volume, or electrolyte balance. For instance, the Ca 2+ ATPase is a pump that transports molecules from the cytosol to the extracellular medium against a concentration gradient, using ATP in the process (Fig. . A). Specifically, ATP phosphorylates the ATPase, causing a conformational change that triggers the transport of Ca 2+ ions. Dephosphorylation of the pump ensures it continuously operates. This sophisticated mechanism inspires the creation of supramolecular pumps to transport molecules directionally across barriers with potential applications in nanotechnology. Examples are pumps that purify water from pollutants or aid in biomedical applications. The field has mainly focused on molecular pumps based on interlocked systems , i.e., systems that pump a non-covalently bound guest from one site to another. For example, a pump that transports a macromolecular ring along a polymer backbone powered by chemical fuel was described. However, there has been a lack of active molecular transport of small molecules and ions across membranes. Notably, two studies recently reported molecular transport, where a cage shuttles ions or molecules from a sender to a receiver phase driven by light. A similar synthetic design driven by the chemical potential of fuels remains a challenge. In this work, we demonstrate the transport of molecules from an aqueous sender phase to an aqueous receiver phase across a centimeter-sized chloroform domain (Fig. ). The transport takes place directionally and against a concentration gradient. The nonequilibrium process operates by transducing high-chemical-potential fuel in the sender phase into a waste molecule with low chemical potential. Notably, we achieve active and directed transport without complex machinery but simply by transiently changing the partitioning coefficient of the transporter molecule by a carbodiimide-driven chemical reaction cycle. Despite relatively poor fuel efficiency, repetitive fueling fills the receiver phase while the sender phase empties. Additionally, we demonstrate that we transport molecules selectively, enabling the sorting of a mixture of molecules in a scenario illustrating Maxwell's Demon thought experiment. Our experimental work, combined with theoretical calculations, provides guidelines for using chemically-fueled reaction cycles to regulate the active transport of molecules across hydrophobic domains.