Comparing the contacts-over-time for h-cisPT before (Figure ) and after (Figure ) substrate insertion, we can observe a general increase in membrane contact following substrate insertion. More specifically, we observe that following substrate addition, the h-cisPT protein does not dissociate from the membrane (as would be represented by contacts going to 0) in any simulations, while dissociation is observed 5 out of 6 simulations of h-cisPT prior to substrate insertion.