Protein bead form factor parametrisation was performed on mature human GSN. The initial model was determined from the PDB entry 3FFN, whose missing loops and N-terminus were reconstructed using AlphaFold2 . Regarding the 56 residues B1, and the 230 residues GFP, the structures are based on the PDB entries 1PGB and 1GFL, respectively. All the structures were prepared with the following procedure. The histidine orientation and protonation states were optimised using Schrödinger Maestro Suite, release 2021-4 . The topology was built using DES-Amber 59 FF and the system was solvated with TIP4P-D The plain MD simulations of GSN, B1 and GFP were also used to evaluate the performance and accuracy of calculating SAS intensities at different resolutions. For nucleic acids, we followed the previous procedure to prepare and perform a 35 ns simulation of the 1,187 nucleotides large subunit ribosome fragment (PDB ID 1Z58) and a 14 ns simulation of single-stranded 12-mer RNA (AGUAGAUUAGCA). The former was used to assess the timing of the SAS intensity calculation, the latter to assess the accuracy.