The next objective is to learn how to make essential changes to the protein structures in PyMOL. The final product from the PyMOL activity of module five left the group with a protein with some missing residues and sequence mismatches in its amino acid sequence because it was aligned on a template. To correct this in the structure, the groups learned how to make mutations to the protein using VMD. In addition, the groups learned how to use EMBOSS Needle, a protein sequence alignment tool. With EMBOSS Needle, the groups noted the incorrect or missing residues and then mutated or added the target residues. Once all the residues were mutated to the correct ones, the groups connected the two heavy chains, two light chains, and two glycans by linking all disulfide and glycosidic bonds. Moreover, the students were able to apply the PDB2PQR tool to determine the titration state of the histidine residues on antibodies at a given pH value. At the end of this process, the groups learned to create one complete full-length antibody structure necessary for conducting MD simulations.