In 2D Fano resonant structures, precision is heavily dependent on the spatial resolution of lithography methods, which are often constrained to planar plate arrays with spacing adjustments. In contrast, the synthesis of colloidal nanostructures via bottom-up approaches is governed by distinct physical principles, offering new possibilities to explore Fano resonance in structures and scales previously unstudied. DNA, an encodable molecule tailored for nanointerfacial engineering, provides a synthesis toolbox with precision spanning from the sub-nanometer scale to tens of nanometers.