Because of the possibility that the template structures used in the AF2 modeling could bias the resulting models, we also generated structures without homology templates. The template-free models resulted in structures with similar pLDDT values compared to those generated with templates; without templates, the highest confidence models maintained average pLDDT values greater than 95 -as compared to above 96 when templates were used (Tables ). Structural alignment of the template-free models showed both templated and non-templated models were very similar. We compared the templated and non-templated models by calculating the root-meansquare-deviation of α-carbon positions (RMSDCα) of the top ranked structures made with and without templates. For GRLMR and SeR the RMSDCα difference between template and without template was 1.357Å and 0.933Å respectively. These small RMSDCα values indicate that use of templates did not bias the resulting models. For downstream analysis, we selected the GRLMR and SeR models with the most favorable pLDDT and PAE metrics from the AlphaFold2 calculations (generated with homology templates).