Figure shows SEM images of the various microporous structures obtained under different freezing conditions. Figure in Supporting Information details the Local Thickness estimation of macropore distributions. The most prominent morphological change is observed between the cryogel scaffold frozen at -12 ℃ when compared with that frozen rapidly in liquid nitrogen. The slow freezing process associated with high-temperature freezing resulted in polydisperse pore structures with large pore sizes (Figure ), while rapid, low-temperature freezing produced closely aligned and layered structures, and narrow pore diameters (Figure ). A sample macropore size calculation of CNC cryogel scaffold frozen at -12 ℃ using the Local Thickness method is shown in Figure .