Evolutionary analysis of natural enzymes can deepen our understanding of life's evolution, highlighting the significance of enzyme combination, utilization, and modification. For instance, Bozhüyük et al. utilized evolutionary insights to develop a method known as XUT ("exchange unit between T domains"), successfully demonstrating a proteasome inhibitor's production by an enzyme complex derived from five distinct systems . This method significantly advances synthetic biology and NP engineering, crucial for clinical drug discovery, development, and optimization, underscoring the importance of evolutionary studies. Similarly, Burkhardt et al.'s analysis of the phylogenetic tree of type I TSs from various organisms led to the discovery of widespread octocoral TSs as a new monophyletic group within class I TSs in the animalia kingdom. This discovery illustrates animals' general ability to maintain and evolve capacities for producing complex specialized metabolites. Burkhardt et al.