We observed that there is large change in the overall secondary structure of Y493H. Three different conformations are observed in the loop-region of the BTB-domain. The loop-region is dominated by the turn while it changes to the coil as the simulation proceeds. We have seen that the residues 417-422 are completely dominated by the 3 10 -helix in Y493H. This pattern of secondary structure is not observed in any other mutants. It indicates that mutation in the Y493H has large impact on the secondary structure as compared to the other mutants.