The SP2/0 myeloma cells and splenocytes from the selected mice were mixed for cell fusion with the assistance of PEG 1500. The fused cells were propagated in HAT medium, and the hybridoma cells were screened for antibody production, followed by subcloning based on the limiting dilution method. Clones with high affinity were cloned three times before ascite production. The above experimental procedures were previously described by our group. The obtained mAbs were purified from ascites for immunoassay establishment using a protein-A affinity column, as described in the Supplementary Information.