ITO electrodes are also commonly applied for the biosensors development due to their high conductivity, wide electrochemical window and ease of modification . However ITO electrodes have poor electrocatalytic properties that are usually compensated for in biosensors development by their modification with catalytically active metals, such as gold or zinc oxide . Such modification is also necessary for the attachment of biomolecules, such as probe DNA, antibody, or aptamers . For instance, Deshmukh et al. ( ) modified the ITO surface with Aminopropyltrimethoxysilane (APTES) creating amine groups on the surface in order to achieve the immobilisation of amine modified probe DNA via a common crosslinker glutaraldehyde. The label-free detection of z3276 gene from STEC O157:H7 was recorded by using EIS. The authors demonstrated detection of chromosomal DNA and achieved linear range between 6.3x10 -16 -3.2 x 10 -14 M and an LOD 2 CFU/mL in potable water samples in less than one hour also including DNA extraction. Two E. coli strains negative for z3276 gene and Bacillus subtilis were detected using the developed biosensor to evaluate the specificity which is a critical parameter for biosensors. were used to confirm the specificity of the sensor.