Compounds 1-28 were subjected to preliminary testing for LXRα and LXRβ activation in hybrid Gal4 reporter gene assays with HEK293T cells. This assay relies on chimeric transcription factors composed of the respective human nuclear receptor ligand-binding domain and the DNA-binding domain of the yeast protein Gal4. Gal4-responsive firefly luciferase served as the reporter gene, and a constitutively expressed Renilla luciferase was used for normalization and to monitor the toxicity of the test compound. This assay also captured the cell penetration and cytotoxicity of the test compounds.