For the second phosphorylation step five nucleoside monophosphate kinases, namely an adenylate kinase (TmAMPK), a guanylate kinase (TmGMPK), an uridylate kinase (TmUMPK), a deoxythymidylate kinase (TmTMPK) and a (deoxy)cytidine monophosphate kinase (Tm(d)CMPK) were identified (Figure ). Additionally, in the genome a pyruvate-phosphate-dikinase (TmPPDK) was found. This enzyme was described before to catalyze the phosphorylation of nucleoside monophosphates directly to nucleoside triphosphates using phosphoenol pyruvate and pyrophosphate as substrates .