Finally, we have computed the complete 3D RNA-bound nucleolin structure using the AlphaFold 3 software, as shown in Figure . Due to nucleolin's complex and flexible nature, obtaining the entire 3D structure of nucleolin using experimental techniques is very difficult. Only the rigid regions of the protein have been elucidated and were used in this study. Based on this structure, in the absence of RNA, the four domains of the protein are arranged in a square shape; however, in the presence of RNA, the protein fluctuates, and to accommodate RNA, the four domains rearrange themselves in a linear shape. In addition, the negatively charged RNA is specifically attached to the positive charged region of all four domains. The positive area of the protein is blue, while the negative is red. The 3D structure of the protein will provide new insights into the RNA binding mechanism of this protein.