The enzyme (Dura or LCC) stock solutions were spun at 14,000xg (4 °C) for 10 min to eliminate protein precipitation. The protein concentration in the supernatant was determined by measuring absorbance at 280 nm using Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies). The supernatant was diluted to a concentration of 1 μM with carbonate buffer (see composition above), and 1 mL of diluted enzyme solution was incubated with either PCL or PET (films or nanoplastics) for 2 days. The incubation temperature for Dura and LCC were 37 °C and 65 °C, respectively. Prior to the photoreforming tests, the solutions were centrifuged at 20000 xg for 10 min to remove any solid residues from the solution.