Manganese ferrite nanoparticles (MnxFe3-xO4 MNPs) have attracted considerable attention for their potential applications in magnetic hyperthermia therapy (MHT) and magnetic resonance imaging (MRI) . The substitution of Fe 2+ by Mn 2+ in the spinel structure can significantly alter their magnetic anisotropy, saturation magnetization (Ms), heating, and contrast efficiency due to the changes in their structural properties, including crystal structure, crystallite size, and lattice parameter . While the impact of the Mn 2+ content on the magnetic properties has been investigated in recent years using many different MnxFe3-xO4 MNPs, a direct correlation between the Mn 2+ content and the magnetic properties is not yet clear.