Three core separations are employed which together capture analytes present in human biofluids with wide ranging hydrophobicity (Figure ). The first is an RPC method for the analysis of a broad range of analytes including smaller more hydrophilic species through to more moderately hydrophobic species. Very polar and ionic species (especially basic analytes) that are not well retained under these conditions are the targets of the HILIC separation. Finally, more hydrophobic species, principally neutral and complex lipid species ranging from lysophospholipids to triglycerides that would not be cleanly eluted from the RPC method, are the targets of an adapted RPC method for lipidomics. The first two are applied routinely to the analysis of urine and blood products, while the latter is generally applied to blood products only (as well as tissue extracts and other clinical sample types outside the scope of this paper). Notes for use, standard operating procedures and proformas are available at .