MS 2 Rescore 3.0 is easy to use and widely applicable MS Rescore can be installed through a one-click installer on Windows, or with a single Pip, Conda, or Docker command on all platforms. The GUI (Figure ) allows any re-searcher to use MS 2 Rescore, regardless of their programming skills. Through psm_utils, twelve different search engine output file formats are currently supported, and through pyteomics, spectrum files are accepted in both mzML and MGF file formats. Currently, five feature generators are implemented: basic, maxquant, ms2pip, deeplc, and ionmob. The basic feature generator adds simple rescoring features that can be derived from any PSM file, such as search engine score, precursor mass error, etc. The maxquant feature generator was specifically written for rescoring MaxQuant 37 msms.txt PSM files, where more rescoring features can be calculated from the spectrum annotations that are embedded in the file. The ms2pip, deeplc, and ionmob feature generators provide machine learning-derived features from MS2 spectrum intensity, retention time, and collision cross section, respectively.