ATS (ammonium thiosulphate) had retarded the hydrolysis of urea when added in a higher amount. It was observed that in soil, ATS converted into active tetrathionate to reduce the activity of urease. Tetrathionate is also required in a higher amount (2500-5000 µg/mL) to inhibit the activity of urease . Soares et al. reported that DCD (10%) treated urea showed that 80% N recovery at a pH value of 6.8, while NBPT treated urea presented an N recovery of 70%. Overall, it is concluded that 0.50% inhibitor concentration showed better results for all three districts. It means that based on results, it could be observed that the 10% urea could be saved in the future with the application of 2-Chloro-6-(trichloromethyl)pyridine and ammonium thiosulfate in combined form. This is not only a big innovation regarding the save of urea and N loss but also for environmental cleanliness.