Numerous SCMS techniques have been developed to perform single cell metabolomics experiments under vacuum or ambient conditions. Among them, a group of methods are based on mass spectrometry imaging (MSI) techniques. MSI has emerged as a powerful technique for spatial metabolomic analysis, offering the ability to map the spatially-resolved molecular information within biological samples. Unlike traditional mass spectrometry (MS) methods, which provide average measurements of metabolites from homogenized samples, MSI allows for the visualization of spatial distributions of metabolites and their abundances directly within tissue sections or individual cells. This spatial information is invaluable for understanding the microenvironmental context of metabolic processes, revealing metabolic gradients, and identifying cellular subpopulations with distinct metabolic states.