Discrimination by visual inspection in the FTIR spectra is not simple because the FTIR spectra pattern is very complex, and the assistance from chemometrics methods is necessary to resolve this issue . The benefit of using chemometrics to interpret FTIR is the ability to relate the spectral pattern to secret information found in a sample . FTIR spectral analysis was widely used alone or in conjunction with chemometrics methods to classify species and discriminate against some closely related species .