For quantitative estimation, we calculated the time difference Δτ between the division timings of B-and C-droplets (Figures 6d): ∆𝜏 = 𝜏 /01_, -𝜏 /01_. , where 𝜏 /01_, and 𝜏 /01_. are defined as the times when rdiv_B and rdiv_C were both approximately 0.5, respectively. As shown in Figure , Δτ > 0 was observed when the RNA concentration difference ∆𝑐̃= 1.25 (i), 0.5 (ii), and -0.5 (iii), indicating that the division occurred through Pathway 1. Alternatively, Δτ < 0 was observed when ∆𝑐̃= -1.0 (iv) and -1.25 (v), indicating that Pathway 2 was selected. These results demonstrated that the division pathway changed depending on the RNA concentration differences, confirming that the concentration comparator for the miRNA sequences worked as expected.