Control group 1 received 0.3 µL of diminazene aceturate as a prophylactic treatment four days prior to infection. Control group 2 was infected with T. b. brucei but received no pretreatment or haemolymph administration. All experimental mice were monitored for 21 days, with parasitaemia assessed every three days. Parasite load was determined via microscopic examination of tail blood at 40× magnification. Additional parameters, including body weight (measured using an Electronic Kitchen Scale, EK3250) and packed cell volume (PCV), were evaluated using the microhaematocrit method (Dacie & Lewis, 1999).