Strains of bacteria would have to be prepared in order to perform this investigation. American Type Culture Collection(ATCC) strains for Gram negative and Gram positive organisms were purchased and utilized in this study. ATCC strains of Enterococcus faecalis(ATCC-29212), Methicillin-resistant Staphylococcus aureus(ATCC-29213), Staphylococcus saprophyticus (ATCC-15305), Escherichia coli(ATCC-25922) and Pseudomonas aeruginosa(ATCC-27853) were isolated and inoculated in peptone water for 2 hours and incubated at 37 °C. Lawn cultures were prepared for each organism on separate Blood Agar(BA) plates. Each of the BA plates were divided into equal 4 quadrants, two of which had antibiotic discs(Table ), one had a 6mm diameter hole punched and one was kept empty for positive control of the growth. 100mg of the Zinc Nanorod powder(obtained from evaporating the ethanol solution) was mixed in 5 ml of distilled water in a vial to prepare a Zinc Oxide Nanofluid. 100µl of the Zinc Oxide Nanofluid was placed in the 6mm punched hole on each of the BA plates. All the culture plates were incubated at 37°C for 24 hours and observed the next day for zones of inhibition .