In the PK/PD study, adult male C57Bl/6 mice with 7-8 weeks of age were dosed orally with compound 37 as a suspension in 1% Tween 80 in 0.5% methylcellulose at 150 mg/kg BID q4h. Terminal tissue collection was conducted at 4, 6, and 10 hours after treatment (11 mice/timepoint). Mice being euthanized for the 4-hour group were euthanized before 2nd dosing. At each collection timepoint, mice were euthanized by CO2 asphyxiation and blood was collected via cardiac puncture into vials containing K + EDTA anticoagulant. Then, brains were extracted, and cerebellum dissected, separated into 2 equal parts then placed into pre-weighed 1.5ml tubes. Terminal CSF was collected for 10h treatment group only. Upon collection, all tissue samples and CSF were weighed, snap frozen in liquid nitrogen and stored at -80 °C for analysis.