For BtPI-PLC (PDB ID 3EA1/2OR2), WTSaPI-PLC (PDB ID 4F2T), and PH domain (PDB ID 1MAI), the proteins were converted to CG representations with an elastic network force constant of 700 kJ mol -1 nm -2 and with an elastic bond upper cut-off of 0.8 nm. Side chain corrections were applied using the scFix option. These CG structures were placed in a box of dimension 8.96 x 8.96 x 15 nm 3 using insane to build the corresponding bilayers. These resulted in 288 lipids for single component The FGF2 protein was placed 2 nm away from the membrane (consisting of 5 mol% PI(4,5)P2 in a POPC background) in 10 different orientations, and each replica was simulated for 10 μs. We coarse-grained the atomistic model from previous