As shown in Figure and Table , the Val11 χ 1 rotamer transition frequency for Val111 decreases for the simulations that start after the T4 Lysozyme Simulations -Backbone RMSDs backbone relaxation (T250, T1000). For the MD simulations, transitions are only observed in the T0 simulation using the original input files. For all three starting points, BLUES exhibits a marked improvement in sampling the rotamer states compared to MD, with MD showing one or zero transitions to the alternate rotamer state. However, as observed in our long MD simulation, there is a decline in rotamer transition rate (and associated move acceptances) for the T250 and T1000 simulations, compared to T0. (Table ). Thus, the rotamer transitions per million force evaluations in BLUES drops from 3.8 to 2.1 after the backbone relaxation and down to 1.3 in simulations starting from the final structure (Figure . This further suggests that some sort of system relaxation is causing an increase in the torsional barrier for Val111. Nonetheless, BLUES still performs favorably compared to standard MD, dramatically accelerating rotamer transitions.