The second fragment growing strategy was the core scaffold fragment, (Figure . B), PDB-ID; 2W6O, that was found to bind to the Lys-A-159, and Leu-A-204. This emerged into the lead compound, PDB-ID; 2W6P, and maintained the same interaction with Lys-A-159, and Leu-A-204, yet gained another Anchor-category residue interaction with Glu-A-201 and a Transient-category residue interaction with Lys-A-202. This resulted in a better IC50 value and total experimental LE. However, the molecule (PDB-ID; 2W6P), also gained multiple Non-hotspot-category interaction residues; His-A-209, Gln-A-233, and Ile-A-287. This means that the ligand grew substantially in size with moderate influence on the efficiency of interaction. This is shown in the LE with only 0.05 difference from the starting fragment.