The copolyester of poly(butylene adipate)-co-(dilinoleic adipate) (PBA-DLA) with 70-30 wt% hard to soft segment ratio was synthesized via two-stage polycondensation method in diphenyl ether using CALB as biocatalyst. Briefly, CALB (10 wt% of total monomers), BD, DA, DLD, and diphenyl ether (200 wt% of total monomers) were added to a round bottom flask and placed into a heated oil bath on a magnetic stirrer. The first step was carried out under inert gas flow at atmospheric pressure and at an initial temperature of 80 °C. After 1 h the temperature was slowly increased to 95° C and the collection of ethanol was monitored for 3 h. Further, oligomerization was conducted under a pressure of 600 Torr for 21 h. In the next step, the pressure was gently reduced to 2 Torr, while still maintaining the reaction temperature at 95 °C for 72 h. Upon completion, the product mixture was dissolved in chloroform and filtered to remove CALB. The obtained chloroform solution was added dropwise to cold methanol under continuous stirring to precipitate a white polymer product. The precipitated product was filtered, washed three times with cold methanol, collected, and dried in vacuo at 40 °C for 24 h.