For IP-HILIC and IP-RPLC an Agilent 1290 Infinity II UHPLC system was used, consisting of a 1290 binary pump (G7120A) containing a V35 jet weaver mixer and a 1290 Multisampler (G7167B) with a draw and eject speed set to 100 and 400 µL/min, respectively. It contained a 1290 MCT (G7116B) with InfinityLab Quick-Connect heat exchangers (P/N: G7116-60015) and a 1290 DAD FS detector (G7117A) containing a Max-Light Cartridge Cell (10 mm; V 1 µL). UV absorbance was measured at 260.0 nm with a bandwidth of 4.0 nm at 10 Hz. The system also comprised two 1290 valve drives (G1170A) containing a 12-pos/13port bio inert valve 200 bars for solvent selection. For the AEX measurements, the same modules were used, except for the pump, which was a 1260 Bio-inert quaternary pump (G5654A). LC-UV-MS measurements were performed on an 1290 Agilent infinity II UHPLC system, consisting of binary pump (G4220A) and a HiP Sampler (G4226A). Moreover, it contained a column compartment (G1316C) and a DAD detector (G4212A). The LC system was hyphenated to a Thermo Q-Exactive Orbitrap MS equipped with a heated electrospray ionization (ESI) source set to negative mode. The following parameters were used: ISCID, 20 eV; capillary temperature, 275 °C; spray voltage, 3.30 kV; Slens RF, 50; sheath gas, auxiliary gas, and sweep gas flow, 60, 10, and 0 units, respectively. Full scan experiments were performed with a resolution of 140.000, 4 microscans, AGC target of 1E6, maximum IT of 200 ms, and scan range of 400-2500 m/z.