The Boxer group used Stark spectroscopy to demonstrate that cobalt-substituted liver alcohol dehydrogenases (CoLADH) exert 𝐸𝐸 "⃗ (𝑟𝑟) favorable for aldehyde hydrogenation, which can be enhanced by introducing the S48T mutation . They observed 𝐸𝐸 "⃗ (𝑟𝑟) in the same direction as the reactive C=O bond, which corresponds to reducing the reaction barrier by stabilizing the chargeseparated TS (Extended Data Fig. ). PyCPET's tools allow us to observe this 3-D 𝐸𝐸 "⃗ (𝑟𝑟) along the C=O bond (Fig. ) and understand what residues contribute toward reactivity. We compute 𝐸𝐸 "⃗ (𝑟𝑟) streamline distributions across MD simulations of WT Co-substituted LADH and S48T Cosubstituted LADH (Fig. ), capturing changes in 𝐸𝐸 "⃗ (𝑟𝑟) throughout dynamics, with an emphasis on the S48 residue influence. However, the representative electric fields in the S48T mutant are similar in geometry to representative WT electric fields (Extended Data Fig. ), a finding that does not clarify the observations that the S48T mutant enhances 𝐸𝐸 "⃗ (𝑟𝑟) along that direction.