β-cyclodextrin (β-CD) was immobilized on the Ox-80 and Plasma samples using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and 4-dimethyl aminopyridine (DMAP) to activate the carboxy groups on the PP surface. Various combinations of the condensation agents and catalysts, such as N,N'dicyclohexylcarbodiimide (DCC), N-hydroxysulfosuccinimide sodium salt (sulfo-NHS), were examined, and the combination of EDC and DMAP was chosen as the optimal condition (Figure ). In the IR spectra of Ox-80-CD, new peaks were observed at 3400, 1640, and 1080 cm -1 , derived from the O-H stretching of β-CD, O-H bending vibrations, and C-O stretching of C-O-C, respectively. These peaks suggested the possibility of esterification. However, little change was detected in the IR spectra of the Plasma-CD (Figure ).