The rotational symmetry component involves three parameters, the 'radius of averaged droplets' (RR) and 'RPD broadening' (RB) describe the spread in the pairwise distances between the proteins around a droplet. The 'amplitude of RPD broadening' (ARB) determines the amplitude of the pairwise distances between the proteins. The localization precision component involves two parameters LP and ALP. The 'localization precision' (LP, denoted as σlp) represents the uncertainty in the repeated localizations of the same protein. The 'amplitude of localization precision' (ALP) determines the amplitude of the pairwise distances between the localizations belonging to the same protein. The arrangement of proteins involves two additional parameters; the 'arrangement of proteins broadening' (AB) describes the spread associated with the pairwise distances of localizations within a cluster. The 'amplitude of arrangement of proteins broadening' (AAB) determines the amplitude of the pairwise distances between the localizations in the clusters (Supplementary Figure ).