The methods were developed for now-conventional ultra-performance liquid chromatography systems with a maximum operating pressure of 15,000 psi. All methods are designed for ideal operation within 80% of this limit, providing some overhead for increases in system pressure often incurred during long sequences of sample analysis. The injection-to-injection cycle time was standardised to exactly 15 minutes across all methods, allowing the analysis of a complete 96-well plate of samples (80 study samples and 16 QC samples) in a 24-hour period.