Metabolomics is a critical field of study for understanding biochemical processes and disease mechanisms at a molecular level. This research area holds potential for uncovering biomarkers for disease , elucidating metabolic pathways , and advancing medicinal research. Despite significant progress in the field, a profound understanding of cellular heterogeneity and the spatially resolved cell-cell interactions remains a formidable challenge in traditional metabolomics studies. Single-cell analysis is essential for various reasons. In complex tissues, cellular heterogeneity plays a crucial role in functions and responses to environmental stimuli. Tumor microenvironments, for example, consist of a diverse population of cells with distinct metabolic states, contributing to cancer progression and drug resistance. Understanding these variations at the single-cell level can reveal critical information about disease progression and therapeutic targets that are otherwise obscured in bulk analyses. Single-cell metabolomics addresses this challenge by providing insights into the unique metabolic profiles of individual cells, which can differ significantly even within the same tissue or culture. Single-cell mass spectrometry (SCMS) is gaining popularity as a powerful tool for single-cell metabolomics studies due to its label-free approach and ability to sensitively detect a broad range of analytes. Unlike traditional metabolomics methods, such as liquid chromatography (LC)/MS, based on bulk sample analysis, SCMS enables direct studies of biological processes of individual cells in heterogenous populations .