In addition to aluminium, iron, copper, and zirconium-based MOFs, we evaluated three biocompatible MOFs (MOF-177 (zinc), CaBDC (calcium), and MgMOF-74 (magnesium)), to investigate their GUV immobilization potential. Zinc MOFs have been extensively studied in biomedical applications due to their compatibility and biodegradability with biomolecules. The zinc based MOF-177 showed viable GUVs being produced and anchored in all three lipid mixtures (Figure , S4c, S5c). The number of GUVs produced with a negatively charged lipid mixture was two-fold more than the other two lipid compositions (Figure ), see supplementary video SV9 for GUV@MOF adduct. The vesicle size with DOPC:DPPC:Chol lipid was relatively large (mean diameter 28 µm) compared to POPC:POPG:Chol and POPC:Chol lipids mix (18 µm and 12 µm, respectively) (Figure , S7). We also observed some oblongshaped GUVs with increased membrane elasticity in addition to perfect spherical GUVs (Figure ). We attribute this to the presence of the zinc cation during the GUV electroformation process. The cellular membrane curvature depends on lipid composition, influence of integral and peripheral membrane proteins, and cytoskeletal modifications. Other than lipid composition our liposome models do not contain other structural influences. In the literature low concentration of ions such as La 3+ and Gd 3+ are reported to affect membrane curvature and induce shape changes in DOPC GUVs. We suspect free Zn 2+ ions are acting in the same way. Since the cations cannot pass through the lipid bilayer, they induce lateral compression pressure on the exterior of the lipid membrane that causes a decrease in the surface area of the external monolayer while the area of the internal lipid layer remains the same. Such differences in the area between membranes of a bilayer cause reversible change in the shape of the vesicle for the duration that the ions are present in the proximity of the liposome. We do observe degradation of MOF-177 over the course of 12 hours in aqueous environments, which is consistent with existing literature. We also tested two alkaline earth metal-containing MOFs. CaBDC only anchored to a negatively charged lipid mixture (supplementary video SV10) and MgMOF-74 did not show GUV anchoring and produced the smallest GUVs among all the MOFs in this study (Figure , S4, S5). Research has shown that calcium ions have a greater tendency to bind negatively charged lipids in membranes than zwitterionic lipids. Therefore, some GUVs were seen anchored to CaBDC in POPC:POPG:Chol lipid mixture despite of low electron affinity of calcium (Figure ). Calcium MOFs are known for high hydrolytic stability because of strong coordination bonding. The robust structural integrity results in the absence of solvated calcium ions that could potentially interfere with GUV swelling. GUVs were successfully produced in the presence of CaBDC with both POPC:Chol and DOPC lipid mixtures but the observed vesicle diameter was reduced (Figure ). There was also no observed GUV@MOF anchoring. MgMOF-74 showed the lowest yield of GUV formation along with the smallest diameter of GUVs formed. A possible reason is MgMOF-74 is unstable under humid conditions, suggesting that it would be very unstable in the aqueous electroformation environment. This is because of the weak coordination bond between magnesium ions and oxygen atoms that dissociate the framework into its constituents releasing metal ions and organic molecules in the environment. We have observed similar results on GUV formation with the zinc -dicarboxylic acid framework MOF-5, which also decomposes in aqueous environments. Such MOFs will find important applications in drug delivery where shortterm degradation of MOF is desired with the released metal ions such as magnesium and zinc being biocompatible.