GROMACS software package is used for all CG-MARTINI simulations. After short energy minimization, the NM system is equilibrated for at least 2 𝜇𝑠 followed by production run of 10 𝜇𝑠 at 310 K. With the last configuration generated after production run, we have rapidly cooled the system to 250 K and equilibrated as a minimum for 10 𝜇𝑠 (since at lower temperatures, systems take longer time to equilibrate). For MMS system even a longer simulation time of about 40 𝜇𝑠 is required for equilibration. We gradually increased the temperature at 20K interval. Thus, we have simulated the NM, MMS and MMU membrane systems at 7 representative temperatures: 350, 330, 310, 300, 290, 270, and 250 K.