Particle size and polydispersity index (PDI) were determined by dynamic light scattering (DLS) using a Zetasizer Nano ZS (Malvern, UK). Liposomes were diluted (to 1 mg/mL) in relevant buffer prior to measurements. For each sample three consecutive measurements were performed at a scattering angle of 173° and at a temperature of 25 °C. For zeta potential measurements the liposomal sample was loaded into folded capillary cells and three consecutive measurements were conducted at a temperature of 25 °C.