to the anti-parallel and parallel β-sheet vibrations, respectively (Figure ). Notably, the positive peak elongation is more pronounced for Gal3C-PEG 550 Da compared to unconjugated Gal3C, and this elongation is further enhanced for Gal3C-PEG 5 kDa. This trend is observed at both waiting times of 150 fs and 1400 fs, the peak elongation introduced by PEGylation could be related to three main factors: the increase of structural heterogeneity and/or the solvent exposure, energy transfer among the amide I modes.