Carbon nanotubes (CNTs) have been extensively studied and utilized in various applications due to their exceptional mechanical and electrical properties. The high surface area of CNTs is one of their major advantages, allowing for a multitude of functions such as chemical and biological sensing, energy storage, battery, tissue engineering, structural colors, MEMS, and medical applications. Despite this, challenges remain in harnessing the full potential of CNTs in novel inventions while producing cost-effective working devices. One of the simplest methods to employ CNT bundles for practical production is through screen printing. Screen printing is a popular method due to its simplicity, low cost, and flexibility, and is being employed for biomedical sensing as transducers to detect biomarkers such as IgG, glucose, DNA, RNA, and others. Other methods to pattern CNTs include vacuum filtration, inkjet printing, spray printing, electrophoresis, and direct patterning. Paper-based microfluidic analytical devices, also known as μPADs, have gained significant attention from researchers for developing various sensing devices to measure different quantities and concentrations of molecules of interest. μPADs are a promising technology for the low-cost fabrication of affordable analytical devices for resource-limited areas such as developing countries due to their accessibility, biocompatibility, disposability, and cost-effectiveness. Compared to conventional substrates, paper offers many advantages such as disposability, lightweight, and flexibility. Moreover, paper has a natural porous structure that enables negative wicking through capillary forces. Therefore, paper can be manufactured with different microfluidic controls to automatically transport microfluids to the functional layers. This feature is particularly attractive for bioassays, which often require multiple reagents and sequential incubations. Hydrophobic and hydrophilic regions can be easily established by patterning hydrophobic materials into the paper matrix, such as wax printing, soft lithography, and paper surface plasmon treatment. These barriers can easily control the dimensions of the device sensing channels. Therefore, μPADs are very promising for simple onsite analysis.