In this work, we built a protocol to generate LNPs with the following parameters: a core hydration level of approximately 12 water molecules per lipid, and a predefined lipid species distribution with a total IL:DSPC:CHOL (for cholesterol) ratio of about 50:10:40 mol%. The protocol constructs a low pH LNP with an inverse hexagonal core, encapsulated 7 strands of RNA with water and neutralizing ions, and a coating consisting of a monolayer along the core channels and a bilayer at the channel ends, closing off the channels. The core comprises IL and cholesterol in a 3:1 ratio, while the coating is made of the remaining IL, cholesterol, and DSPC. These considerations are based on experimental data for an MC3 LNP .