Latin America is a region with an extraordinary biodiversity and richness in endemic species. It is a region that may be home to at least a third of global biodiversity. Brazil, for example, is considered to host the earth's richest flora, with at least 50,000 species or one-sixth of the planetary total. Another example is Ecuador with its mega-diverse flora comprising more than 25,000 plant species (and thus twice the number of plant species found in Europe). Ecuador, also has the highest vertebrate species density worldwide. Therefore, Latin America is a major source of bioactive compounds. Moreover, it has been reported that several databases contain NPs isolated and characterized in Latin American countries. More than 92 molecules with therapeutic effects have been identified from Latin American NP databases. Just recently, a NP database from Argentina and Colombia were published. In 2023 was published the first version of LANaPDB, a compendium that aims to gather and standardize the NP databases of Latin America which was already included in COCONUT (). In early 2024, and update was reported regarding the NP-likeness profile of the database. Herein, we report a major update of LANaPDB, a compound collection that aims to gather and standardize all the Latin American NP databases. The analysis of the database includes the structural classification of the compounds, calculation of six physicochemical properties of pharmaceutical interest, visualization of the chemical space, quantification of the structural diversity, molecular complexity, synthetic feasibility, commercial availability, reported and predicted biological activity.