We believe that further exploration of this principle will result in methodology that fully exploits the rich structural repertoire of DNA-based interactions, and can greatly expand the scope of bottom-up fabrication in general. The scope of HS binding motifs may potentially be broadened with non-canonical bases, thereby allowing TFOs to target and organize any dsDNA sequence. Finally, understanding the folding properties of dsDNA also has biological relevance beyond in vitro nanotechnology, as implied by the spatial organization of genomic DNA by triplex-forming long noncoding RNA.