How these excited states are influenced by the environment requires elucidating the alignment of within the membrane. Furthermore, we also measure the integration of the hydrophobic chromophore core (fbf) in the DPPC membrane with confocal microscopy, which does not show a half-moon shape, presumably due to a random orientation of fbf within the lipophilic part of the membrane. Further exploration of the alignment of [1] 2+ in the membrane can be found in the SI section S6. The transmembrane alignment of [1] 2+ is ensured by its geometry matching with the geometry of the membrane.