P values were calculated via paired sample T tests, to investigate any seasonal effects of AAs and ARGs. By combining sample data, the seasons considered were winter (November '18, December '18 and January '19), spring . Statistical significance in all tests was defined as p ≤ 0.05. Pearson correlation coefficients were used to investigate potential relationships between ARG and AA abundance. Positive correlations were considered >0.5 and negative correlations were considered <-0.05. Statistical significance testing was performed to highlight correlation results that were significant.