Typical immobilization levels were 1 ± 0.2 nm for monoclonal antibodies, and 2 nm for rabbit polyclonal antibodies. Following the load step, all sensors were equilibrated to baseline for 120 seconds in 1× Kinetics Buffer. An association step was performed for 300 seconds with analyte at 100 nM quantity, followed by 300 second dissociation into 1× Kinetics buffer.