For the Michaelian type of reaction mixture, the relation between the initial rates and the concentration of the substrate is nonlinear; usually a polynomial-the quadratic kind-is the outcome with a higher coefficient of determination than the linear regression outcome. This is not to imply that with appropriate software (graph pad, for instance), a hyperbolic curve is no longer relevant. Thus, a factor, φ is introduced in the following relation to account for nonlinearity. This is intended to address a situation in which the substrate concentration, [S0], is ≫ the enzyme concentration, [E0], such that the following equation may hold.