Similar observations were found for gels which were normalized and loaded in each lane based on total protein content (i.e., 20 µg per lane, see supplementary information). Across all polystyrene nanoparticle types examined, a change in the protein corona composition was observed following incubation under various shear flow conditions. Across all nanoparticle chemistry examined, the introduction of shear flow conditions at 0.85 cm/s led to an increase in the number of bands observed and their corresponding intensity, suggesting the importance of examining protein corona formation under physiologically relevant flow conditions. We also observed time-dependent changes in the intensity of gel bands and relative composition of protein between 2-and 24-hour incubation timepoints. This observed effect was more pronounced for unmodified and amine-modified nanoparticles.