Recognizing that many food monitoring systems employ oligonucleotide probes, we immobilized fluorophore-labelled, single-stranded DNA oligonucleotides onto transparent polyethylene food wraps, which were then imaged with target food products. To this end, DNA oligonucleotides labelled with Pacific Blue, FAM, Cy3, and Cy5 fluorophores -compatible with DAPI, FITC, TRITC, and Cy5 channels, respectively, were deposited onto the polymer substrate via microcontact printing. Immobilization via physical adsorption was employed to eliminate the possibility of confounding fluorescence induced by crosslinking reagents.