Deeper studies are required to address remaining fundamental questions about the factors contributing to power overshoot in MFCs and ensuring that the measurement does not influence the results. Of specific interest is the role of an improper measurement in producing overshooting power density curves and, relatedly, the connection among acclimation, time to reach stability, and the measured performance. In designing such studies, pure-culture G. sulfurreducens could be an ideal EAB because it can simplify interpretation (compared to mixed-species EABs) by eliminating fluctuations in interspecies populations and by limiting electron transfer to direct mechanisms only. Precise control over operational conditions is also required to probe the overshoot effect and potentially cure it. Microfluidic microbial bioelectrochemical systems have been used to study the effect of flow rate, chemical composition of the nutrient solution, and other experimental conditions on measurable performance indicators, such as EAB electrical outputs power production, kinetic parameters, and EAB pH. When applied to MFCs, certain efficiencies can be realized in a microfluidic format, such as reduced start-up time, ability to eliminate the need for ion exchange membranes, optimization of biofilm formation and improved outputs and assay studies via high parallelization. The recent availability of microfluidic MFCs that are reliably operational over a timescale of months provides the opportunity to finally study overshoot in mature MFCs vis-a-vis solution concentrations, convection and other related hydrodynamic effects under highly controlled conditions. In addition to eliminating the concentration cycling that occurs in batch-fed MFCs, continuous-flow microfluidic electrochemical cells can allow accurate prediction and manipulation of concentration gradients, thus presenting an opportunity to validate whether overshoot is indeed correlated to nutrient availability. A microfluidic approach can also be used to manipulate the EAB via controlled application of shear stresses over a large range of values.