A simpler method of using the TPP on Linux machines with Docker () already installed (or if it can be installed) is to use our prebuilt Docker images. Docker is a containerization system that allows complex sets of tools to be pre-configured by the maintainer and then trivially run by anyone with the Docker system installed. Thousands of bioinformatics tools are available via Docker, often as part of the BioContainers infrastructure. The TPP Docker container uses the BioContainers scheme to provide Docker users with the ability to run the TPP, either in command-line mode via docker run commands or via the GUI by launching the web server in the container, with no installation required. Docker even runs under Windows and one can run the Linux version of the TPP under Windows when Docker is installed there.