Following the results from the parameterization, we investigated the aggregation of eight disaccharides using the new Martini 3 parameters and compared the results to atomistic control simulations performed using the GLYCAM-06j force field. We performed simulations comprising a homogenous 50 gL -1 solution of the disaccharide of interest in water. At this concentration, we expect all of the disaccharides to be readily soluble in water (see methods for further details).