The results of weighted least-squares fit of the k obs values listed in the second column of Table to Eqn ( ) are shown graphically in Figure . Both rate constants k on and k off as well as the enzyme concentration [E] 0 were treated as adjustable model parameters. Non-symmetrical confidence intervals, at the 95% likelihood level, were computed according to the profilet method of Bates & Watts . The numerical results are summarized in Table to Eqn . For details see text.