Data reduction parameters optimized for raw, un-adjusted diffraction frames were used unchanged for processing of their CL-adjusted counterparts. CL adjustment of diffraction frames improved their dynamic range, particularly for low-resolution reflections (Supplementary Figures ). The consistency and accuracy of adjusted reflections were judged in part by data reduction statistics (Figure ). Analysis of twin law tests for unmodified and CL-adjusted EBEC MicroED data from salen ligand and biotin crystals illuminated with low fluence showed uniformly reduced evidence of pseudotwinning after CL adjustment, consistent with its anticipated improvement of dynamic range (Figure , Supplementary Table ). However, applying the CL adjustment to higher flux (0.045 e -/Å 2 /s) and fluence (2.25 e -/Å 2 ), salen ligand and biotin crystal datasets generally yielded negligible changes to the estimated twin fraction. In most of these cases, CL adjustment decreased the estimated twin fraction, albeit slightly, and only in the case of one salen ligand crystal did we observe a negligible increase in the estimated twin fraction upon applying the CL adjustment.