Immediately after incubation, 20 µL of incubated AAV sample was loaded manually into the sample well, that had previously been emptied after BGE prime. The AAV analysis was performed with a 5minute run time per injection. For each of the samples, 5 separate injections were performed per 20 µL sample load. The sample well was rinsed and a BGE refresh was performed after all injections of a sample were run.