The arrangement of individual components greatly influences the optical output in the resultant assembly, regardless of whether the supramolecular chirality results from the combination of chiral/achiral subcomponents. While achiral molecules in a mixture of chiral and achiral components can be forced into chiral assemblies under the influence of strong interaction between both, chiral molecules generally prefer to form distinct chiral structures with specified supramolecular chirality. On the other hand, assemblies with exclusively achiral molecules, can generate supramolecular chirality by their stereospecific arrangements in 3D space, but generally are racemic. Thus, proper characterization of the resulting assemblies to gain insight into their absolute configuration and enantiomeric excess (ee) is of utter importance. The direct observation of chiral morphology in supramolecular assemblies has become feasible due to the rapid development in sophisticated microscopic techniques, including scanning tunnelling microscopy (STM), atomic force microscopy (AFM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). In contrast, spectroscopic techniques such as Raman optical activity (ROA), circular dichroism (CD), and vibrational circular dichroism (VCD) offer insights into the direct stereochemical imprint. Although single-crystal X-ray structural analysis is one of the most viable methods of determining the absolute configuration and understanding the self-assembly process, obtaining appropriate single crystals of these assemblies is a potential problem. Herein, we emphasize on the CD spectroscopic analyses, a frequently employed technique for the assessment of chirality.