Overall, the results suggest successful transfection of the PTH1R receptor in both conditions (24h and 48h). A stable cell line of PTH1R overexpressing HEK293 cells was created by selecting of transfected cells with 600 µg/mL G418 (as part of the complete media). Successful creation of the cell line (HEK-PTH1R) was confirmed by Western Blot. A stable cell line was created by selection of transfected cells with geneticin. Figure .3a shows that stably transfected HEK293 cells (HEK-PTH1R) present a stronger PTH1R signal. Densitometry further revealed higher ratios (>0.3) compared to control cells (average: 0.22), however, as this analysis was not conducted in triplicates no judgement on its significance can be made. Further, the observation of anti-Flag-Tag bands in the stably transfected cell lysate is further indication that the selection of PTH1R overexpressing cells was successful.