In the previous section, we have already recommended optimal values for various parameters involved in ABFE calculations. Here we additionally suggest a "minimum" value for each parameter that may be used in cases where computational resources are limited and/or cost-cutting is desired (for instance, coarse-grained applications at large-scale). Table summarises all our recommendations. We urge practitioners not to go below the "minimum" value in any case to ensure reliability of results. We acknowledge that there is no one-size-fit-all solution for all systems given the huge variability in proteinligand structures. Therefore, our recommended protocol is kept flexible such that various parameters will need to be adjusted in order to fulfil two criteria of reliability. The first criterion is bringing the standard errors (using independent estimates from ensemble simulations) below a pre-defined threshold value. The second one (specific to the NEQ method) is to ensure that the distance value is below a preset threshold value. We suggest using 0.5 kcal/mol as the threshold for standard errors and zero for the distance value which are assumed in our following discussion. However, users may choose their own values based on their requirements, preferences and error tolerance.