Results also exposed that the minimum urea hydrolysis of about 2.41, 2.79, and 4.68 IU/g soil was observed after 4 th -day incubation, with the rate of 0.50% for Faisalabad, Gujranwala, and Sheikhupura, respectively. While the control showed 25.43, 19.13, and 25.87 IU/g soil inhibition for the same districts, which indicated that A1 combination presented the minimum urea hydrolysis results as compared to control for all three districts. Hydrolysis of urea decreased as the incubation period increased. The average reduction in urea hydrolysis was observed between 1-15 th days, after that urease enzyme slow downed its activity. From 14 th to 37 th days, no significant (P > 0.05) results were obtained because the activity of urease in soil treated samples was restored due to inhibitors' potential losses. However, control (untreated) disclosed a high amount of urea hydrolysis as compared to 0.50% concentration of inhibitors in all three districts treated soil from day 1 st to 15 th .