Discovery proteomics is a powerful tool for understanding cellular biology, supplying information on cellular status through identification and quantification of proteins. The physical, analytical, and computational work required for discovery quantitative proteomics means that it is not suitable for routine analysis. Translating discovery proteomics results into a targeted MRM assay provides exquisite detail on altered pathways in a simpler assay. By taking this approach, we can report on key processes that in turn inform on the cellular status of bioreactors during production of mAb from CHO cells. In doing this we translate biological understanding gained from discovery proteomics into a rapid, quantitative, and simple to perform assay with easily read data.