PARK7 is a chaperone-like peptidase that can repair proteins damaged by a series of aldehyde products, including methylglyoxal and glyoxal. PARK7 specifically protects GAPDH from cysteine and lysine adducts, including glycerate damage caused by metabolic products generated by GAPDH itself . PARK7 is also significantly destabilized after propachlor treatment and thus could be inactive. A cellular assay designed to quantify PARK7 ability to deglycate glyoxal modified proteins in HEK293T cells has been previously established . We measured the ability of endogenous PARK7 to degylcate glyoxal-modified proteins after incubation with 1 mM propachlor for 30 minutes (Figure and Figure ). In the presence of propachlor, the intensity of proteins converted to carboxy-methyl-lysine after glyoxal treatment increased significantly in comparison with the control experiment (DMSO vehicle treatment).