To conclude, our findings suggest that coumarin-30 is a valuable tool for studying tubulinligand interactions. Its application in the MST technique provides a convenient, sensitive, and accurate method for screening and classifying diverse tubulin-targeting compounds. The development of inexpensive and informative express methods like the C-MST assay holds promise for accelerating the pipeline for the discovery of novel tubulin binders. The assay's capability to sort ligands could be crucial in identifying small molecules that bind at diverse sites on tubulin, facilitating development of new drugs to address resistance to existing cancer chemotherapeutics. valuable comments and assistance. Fluorescence anisotropy measurements were performed using equipment funded through the federal project "Infrastructure Development for Scientific Research and Personnel Training", part of the national program "Science and Universities".