The response of LPO2-Ade38090/Glu30010 was finally enhanced to ∆R/R0 = -14.3% by increasing the thickness of the initial precursor coating to 100 µm (Figure ). This is owing to the tremendous material loss and the associated reduction of the mean thickness of the sensor by a factor of around two upon laser-treatment. Coatings with insufficient thicknesses of 45 µm or even 75 µm hardly allow for the envisaged formation of an efficient two-layer sensor heterostructure by preserving active N-sites from the adenine-CNFA precursor because of severe pyrolysis and graphitization of most of the films. However, further increase of the thickness leads to delamination (e.g. upon rinsing).