Confocal imaging was conducted by using an upright Leica TCS SP8, equipped with internal Hybrid (HyD) detectors and Acusto Optical Tunable Filter (Leica microsystems CMS GmbH, Germany). Dye excitation was performed using a 561 nm laser at 1.0 % power. Emission signal was collected using an internal HyD detector at the range of 571-649 nm. Images were acquired using the galvometric scanner with a 20X air objective (HC PL APO 20X/0.75 CS2) providing images with FOV = 123.1 mm, pixel size = 0.152 mm. Z stacks were acquired using the galvo stage, with 0.281 µm intervals. The acquired images were visualized using ImageJ.