We further evaluated our method to sample rotational and translational degrees of freedom by applying MolDarting to sample the binding modes of toluene bound to T4 lysozme L99A. Toluene exhibits four binding modes when bound to T4 Lysozyme L99A. These binding modes vary by rotational and translational degrees of freedom; two are distinct and vary by a rotation, and the other two binding modes are symmetry-equivalent to the first pair. We applied MolDarting sampling with BLUES to the non-symmetric binding modes of toluene. The populations of the two binding modes were selectively sampled using MolDarting, without sampling the non-symmetric binding modes (Figure ). MolDarting also was able to recover the correct populations of the binding modes, with the correct population split being 60:40, and our triplicate runs giving 58% ± 3% for the dominant binding mode and 42% ± 3% for the less populated binding mode. The acceptance rate for these moves over these trials was approximately 22%, which is roughly two times the acceptance rate for random center of mass moves we explored in the original BLUES paper, which further shows the benefit of targeted moves.