The generated composite particles, mRNA-PEI@ZIF-8 (mRPZ), underwent treatment with RNase A and exposure to biological media (Figure ). When comparing mRPZ to RNase-treated mRNA, minimal to no degradation of the mRNA was observed, as the band remained approximately the same length as the RNA control. This validates that mRPZ shields the mRNA from RNase as well as mRNA@ZIF-8, a crucial requirement for effective gene delivery. Following exposure to biological media, the isolated mRPZ pellet was analyzed on a native RNA gel, revealing a distinct band of mRNA present for all biological media for up to 4 hours. This confirms that mRPZ is stable longer than mRNA@ZIF-8 in biological media. This improved stability can be attributed to the incorporation of PEI as the mRNA@ZIF-8 alone was not stable.