All dSPE, LLE and SPE preparation protocols were performed using seven replicates of the pooled EDTA plasma. Aqueous extracts from the proposed dSPE method, SPE plates, and LLE aqueous extracts were analyzed by C18 RPC+/-. Evaluation of the LLE and SPE protocols against the dSPE technique were based on recovery measurements for annotated LMWMs using the R package peakPantheR .