developed for the rapid, sensitive, and portable detection of human serum albumin (HSA) for the first time. The paper-based ME biosensor uses the magnetoelastic effect of NiFe2O4 nanoparticles and the excellent mechanical properties of paper to transform the surface stress signal induced by the specific binding of HSA and antibody modified on the paper into an electromagnetic signal. By optimizing the concentration of NiFe2O4 nanofluid and the impregnated numbers of the NiFe2O4 nanofluid-impregnated papers, the biosensor's sensitivity has been improved. The experimental results have demonstrated that the biosensor exhibits a linear response to HSA concentrations ranging from 10 μg•mL-1 to 200 μg•mL-1, with a detection limit of 0.43 μg•mL-1, significantly lower than the minimal diagnosis limit of microalbuminuria. This NiFe2O4/paper-based ME biosensor is easy to fabricate, highly sensitive, and selective, providing a valuable analytical device for early monitoring and clinical diagnosis of microalbuminuria and nephropathy. This study highlights the successful integration of the paper-based biosensor and the ME sensing analytical method, providing a highly-sensitive, easy-to-use, disposable, and portable alternative for point-of-care monitoring. This work demonstrates a new and simple method for ME biosensor design by exploiting the unique properties of NiFe2O4/paper, and provides a new idea for the application of cellulose paper in the field of ME biosensors, making it an effective method for early prevention of diabetes and diabetic complications.