Larvae were then anaesthetised on ice for 5 to 10 min before being placed in individual prefilled 2 mL tubes with 3.0 mm diameter zirconium beads with 1000µL PBS. They were then macerated briefly using the BeadBug 6 Homogenizer (Benchmark Scientific, USA) in two 45 second intervals at 4350 rpm. After homogenisation, serial dilutions of 1:100, 1:1000, and 1:10,000 were then made for each larvae/tube and 100 µL of dilution was spread plated onto SDA plates with 50 ug/mL chloramphenicol. Plates were then incubated at 27C for 48 hours before CFUs were counted.