The APL vor was calculated only for the leaflets in contact with water, see Supplementary material for further description of the analysis. Membrane thickness values were obtained from the average membrane electron density profile peak values along the z-axis with the assumption that the membrane is planar and its surface is perpendicular to the z-axis. Thus, the membrane thickness describes the overall thickness of the whole membrane structure with all lamellar layers considered. The thickness of the individual bilayers was also determined for the double bilayer configurations; the distance considered in this case extended from the outer leaflet peak to the electron density peak at the center of the membrane.