The data above shows chemical fuel can induce a morphological transition from vesicles to oil droplets to smaller vesicles. However, the intermediate oil droplets are destroying the original vesicles, and the process is too drastic to call it self-division. We hypothesized that smaller amounts of fuel could prevent the complete collapse of the vesicles into oil droplets and, instead, lead to a bilayer swollen with some oil. The local production of fatty acids through the oil's hydrolysis could produce new vesicles. Such a process would be closer to self-division. Following this approach, we added less fuel to the multilamellar vesicles (10 mM EDC, 65 mM C10, pH 4.9). Data obtained by confocal microscopy showed that activation of the precursor results in a moderate increase in fluorescence intensity of the vesicles surface, indicating a slower minor conversion of the precursor to anhydride (Fig. ; Movie S3). Importantly, the multilamellar vesicles mostly stayed intact instead of fully converting to an oil droplet. Surprisingly, after 8 min, we could observe the formation of thin, unilamellar membranous structures on the surface of the multilamellar vesicles, which slowly grew into elongated compartments (Fig. ). These new membranous structures remained attached to the mother vesicle for more than 7 min until they finally separated from the mother vesicle. This process of producing daughter vesicles continued for 15 minutes. The division behavior was heterogeneous as some vesicles produced many small daughter vesicles, whereas others produced only a few. The heterogeneity is likely a result of localized variations in the concentration of reactants and the structural integrity of the vesicle membrane. Observing these varying growth rates provides valuable insights into the mechanistic aspects of vesicle division, highlighting the complex interplay between chemical kinetics and membrane dynamics (Fig. , Movie S4-S5).