We used the morphological profiling data from the in-house Cell Painting Assay (CPA) to identify lysosomotropism in small molecules. We applied the lyso score to quantitatively score lysosomotropism. We confirm that mere presence of a compound in the well-established cross-section window of logP and bpKa values doesn't su ce it to be a lysosomotropic compound. By performing a MMPA, we were not able to detect key substructures that can be made responsible for a lysosomotropic e↵ect.