Timescales for the third to sixth eigenvectors are similar for each trial, however t 2 clearly increases with decreasing VAMP2(2) score. The second-ranked model is omitted for clarity because it does not follow this pattern. We suggest the reason for this behavior is due to the fact by enforcing reversibility in the estimation of the transition matrix it is difficult to get numerical consistency between the three count matrices (C 00/0t/tt ) and the eigenvectors (U/V) in Equation .