Western blot was performed to study the expression of some of the proteins of interest (Figure ). CD63 and CD81 were investigated due to their EV specific nature and to confirm the presence of these which was indicated from FACS. It was observed that both CD63 and CD81 were much more abundant in the CE-EVs compared to the CE-cells (Figure ), supporting the presence of membrane-bound vesicles in the sample. Calnexin, on the other hand, is an endoplasmic reticulum (ER) protein used as a negative staining since it should not be present in the CE-EVs.