Table | Banked samples were used to compare performance of select anti-nucleocapsid antibody pairs in LFAs. In total, six RT-qPCR-confirmed SARS-CoV-2 positives, three SARS-CoV-2 negatives, and two potential coronavirus cross-reactive samples were screened. The robotic screening system automated the first screening round against GM-rNP at 50 ng/mL in the round's positive tests, and a buffer control in the round's negative tests (Round 1). Subsequently, the robotic screening system automated three more rounds against AB-rNP at concentrations of 50, 25 and 10 ng/mL in each round's positive tests, and a buffer control in each round's negative tests (Rounds 2, 3, and 4, respectively). We stepped down the concentration of the AB-rNP in three successive rounds because as vendors developed (and we sourced) new α-NP antibodies, better antibody pairs emerged and the average signal intensity in the LFA tests reached the non-linear region of the response curve.