We used four samples of oxylipin extractions from human blood serum and three from microalgal biomass to demonstrate the practical application of NEO-MSMS and oxPUFA data with real samples. Serum samples had been processed for the quantitative profiling of oxylipins following our extraction protocol (including the KOH saponification and the SPE like the oxPUFA samples described above) and the extracts stored at -80 °C. Blood samples of healthy donors were obtained from EFS (Etablissement Français du Sang, Montpellier). All procedures in use by the EFS are defined by the law and follow institutional guidelines.