The CPA is a routine in-house screening assay at the Compound Management and Screening Center, Dortmund, and is used in identifying numerous biological clusters and pathways, among them lysosomotropism. The similarities between CP profiles can be measured by Pearson's similarity. The CP profiles are considered similar if their Pearson's similarity values are greater than 75%. Schneidewind et al. (2021) identified a biocluster in the CPA data whose mode of action is likely due to disturbed cholesterol homeostasis caused by lysosomotropism. Smoothened agonist (SAG), a well-established lysosomotropic compound, is present as a reference compound in the dataset and can be found in the reported biocluster. Because of its pronounced profile in the CPA, SAG was used as the reference compound for defining the lysosomotropic phenotype. This similarity score, termed as the lyso score, ranges from 0 (indicating no biosimilarity) to 100 (indicating full biosimilarity). Compounds with a lyso score above or equal to 75 were annotated as lysosomotropic given the high biosimilarity of their profile to the profile determined for SAG, the rest were labelled as non-lysosomotropic. Out of the 2065 compounds present in the PhysChem window, 1327 were labelled as non-lysosomotropic and the remaining 738 as lysosomotropic.