The suspension of LUVs was prepared as described below. The required volume of chloroform (Merck, Darmstadt, Germany) solution of POPC (1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine; Avanti Polar Lipids, Alabaster, AL) was transferred to a glass test tube. The solvent was evaporated under a stream of nitrogen and the lipid film was kept under vacuum overnight. The dry lipid film was hydrated with Mili-Q water (Milipore, USA). After 4 minutes of continuous vortexing the suspension of multilamellar vesicles (MLVs) was extruded with a mini extruder (Avestin, Ottawa, Canada) through a polycarbonate membrane (Whatman; Little Chalfont, UK) with a nominal pore diameter of 200 nm. The lipid concentration was 1 mM and the LUVs suspension was used at different dilutions later on.