As for the collision energy influence, similar TP and FD rates were observed, namely 97.5 and 70.2% for the lower collision energy and 96.3 and 70.4% for the higher collision energy, respectively (Figure ). However, a difference in reduction rates was observed, which was 15.8% for the low collision energy and 12.3% for the higher collision energy. When these results are compared with the trend of decreasing reduction rates for higher CNLs masses, it is again confirmed. The lower collision energies, in general, create higher m/z fragments, which corresponds to lower CNL masses, and, hence, performs better in eliminating TN fragments, yielding a higher reduction rate than the higher collision energy fragments.