Biomass from stationary-phase batch cultures was harvested by centrifugation at 8,500 ×g, lyophilized and stored at -20 ºC until extraction. The freeze-dried biomass (42 g) was extracted with MeOH (6 × 500 mL). The resulting crude extract (7.8 g) was fractionated by C18 reversed-phase Vacuum Liquid Chromatography (VLC) using a gradient of decreasing polarity -10%, 30%, 50%, 70% MeOH (aq), 100% MeOH, 100% iPrOH and 100% CH2Cl2), yielding a total of seven fractions (A-G). After each separation during the isolation process, an LC-HRESIMS analysis was performed in order to check in which fraction the target compounds were present. The fractions D-F showed to contain the microginins with higher abundance.