Microorganisms, though unseen by the naked eye, play a significant role in causing various diseases. Consequently, the search for antimicrobial agents becomes crucial in combating these issues. Secondary metabolites identified from P. sclerotiorum extract that possess antimicrobial properties. Penicilazaphilone C (33) displayed potent antibacterial effects against Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus, surpassing the antibacterial activity of Penicilazaphilone B (32). This compound emerged as a new azaphilonidal derivative, exhibiting strong antimicrobial properties along with selective cytotoxic effects. HLL113. These compounds, obtained from yellow oil, showed solubility in methanol and chloroform and demonstrated α-glucosidase inhibitory activity. Regarding phytotoxicity, compounds such as isochromophilone H (12), sclerotiorins A (58)