Besides that, it is unable to analyse specimens with thickness > 200 nm ; TEM operates also at a high vacuum which renders many soft polymeric or biological samples mechanically unstable. Furthermore, soft polymeric or biological samples, not being conductive, accumulate charge on their surface when electrons bombard on them leading to distortion at their surface, usually termed as drift . Application of AFM for studying kinetics of evolution of a species like nanoparticles on soft solid/liquid interfaces is limited by the probe size . Softness and stickiness of the surface too makes application of AFM difficult. In many situations, the reaction occurs within a confined space which does not allow direct access to the AFM tip.