The 20 ml fractions of the two CPC experiments (elution and extrusion) were characterised by TLC. TLC was performed on pre-coated silica gel 60 F254 Merck plates, with the migration solvent system consisting of ethyl acetate/toluene/acetic acid/formic acid (4/6/1/1, v/v). Compound migration was visualised under UV light at 254 nm and 366 nm and revealed by spraying consecutively the dried plates with 50% (v/v) H2SO4 acid and vanillin (10 g / l) ethanolic solution followed by heating. As a result, six fractions were obtained from CPC1. The first fraction (consisting in the seven first elution minutes (t0 of the experiment)) was used for CPC2. The other fractions were noted F08, F09, F10, F11 and F12 (Figure ). The fractions from CPC2 were arranged in reverse order of collection (because CPC1 was performed in descending mode while CPC2 was performed in the ascending mode) to obtain a chemical profile continuity between the two fractionation steps. The fractionation of the bark extract resulted in twelve chemically simplified fractions of polarity ranging from the highest (F01) to the lowest (F12) (Fig. ), ten produced by elution and two by subsequent column extrusion. The fractions were then analysed in parallel by LC-HRMS 2 and 13 C NMR. Experimental spectral data were then processed and annotated while taking advantage of purposely created databases. A comparison of the resulting candidate structures from both workflows was carried out to enhance confidence in the final profiling outcomes.