Compounds 24-25 showed selective antibacterial activity against the Gram-positive bacterium, S. aureus using disk diffusion method at a concentration of 30 μg/well , whereas the same study revealed that compound 24 had a selective cytotoxic activity against human CNS cancer cells XF-498 with IC50 of 22.39 μg/mL. In addition, averantin and its derivative 1'-Omethylaverantin ( ) displayed a weak antitumor activity against the bone marrow cancer cell line K562 at a concentration of 100 μg/mL . Another study mentioned that compound 27 exhibited modest cytotoxic activity against human glioblastoma SF-268, human breast adenocarcinoma MCF-7 and human large-cell lung carcinoma NCI-H460 cell lines with IC50 value ranging from 33.59 to 44.22 μM, whilst compounds 26 and 28 displayed weak to moderate cytotoxic activity against MCF-7 with IC50 value of 45.47 and 29.69 μM, respectively . Also, compounds 26-27 displayed potent antioxidant activity, whereas compound 28 exhibited weak antioxidant activity in terms of antioxidant capacity compared to Trolox suggesting that the presence of oxygen in the side chain of the anthraquinones may play role in their antioxidant activity.