We then investigated the fate of the nanogels after their internalization by the cells. First, we studied colocalization of FITC-labeled lipids with NG-633 at different time after their internalization by the cells. We found that 30 minutes after delivery, fluorescent signal corresponding to FITC-lipid could be colocalized with the NG-633 fluorescent signal, suggesting that NG-633 and FITC-lipid are still associated. After longer period (5 h and 26 h post incubation with FITC-LPX-633), only fluorescent signal corresponding to NG-633 is observed inside cells.