We determined whether the transporter molecule transports from the sender to the receiver after being activated to its corresponding anhydride by chemical fuel. Thus, we added 100 mM EDC to 10 mM Cbz-D in the sender phase (Fg. 3A). Over 8 days, we took daily aliquots from the sender and receiver phases and measured the amount of EDC and Cbz-D using high-performance liquid chromatography (HPLC, Fig. ). We found that, within an hour, all EDC was consumed, and 5.0 ± 1.2% Cbz-D partitioned into the chloroform phase (Fig. , Fig. ). Over 5 days, 2 ± 0.3% Cbz-D transported to the receiver phase, and 97 ± 1.3% Cbz-D remained in the sender. This result implies a four-fold greater transport than the nonfueled. As more fuel implies more anhydride formation, we hypothesized it should control the amount of transported Cbz-D. Indeed, we found that by fueling with various fuel concentrations, we can regulate the amount of transported Cbz-D between 0.64 ± 0.05% and 5.4 ± 0.8% (Fig. ).