Inhibitors were subsequently administered orally 1 hour later. In the case of Cxcl1, all inhibitors led to significant reduction of chemokine expression (Figure ). Interestingly, iBETBD2 had no impact on Ccl2 and Cxcl2 levels, indicating a more selective effect on gene expression. Due to variability in the APAPtreated samples, statistically significant reduction in Cxcl2 and Ly6G was not reached for any inhibitors, however, I-BET151 and 3 reduced cytokine levels comparable to untreated cells. While BRD4-D1 selective inhibitor 1 reduced the expression of all investigated chemokines, 2 reduced expression of all but CXCL2. These effects did not reach statistical significance however, nor did they reach baseline levels of the untreated controls (Figure ).