Chen et al. ( ) achieved even higher sensitivity with a LOD of 10 CFU/mL in less than two hours. In this study, a sandwich-based structure with antibodies modified magnetic beads on one side and aptamers on the other was used for STEC recognition. In the presence of STEC in the sample, the CRISPR/Cas12a complex was triggered leading to hairpin DNA cleavage and effectively signal alteration, detected with DPV as well. Another study by used the CRISPR-Cas12a system coupled with EIS to detect E. coli and S. aureus. In their work, a gold disc electrode was modified with thiolated probe DNA, and the detection principle was schematical presented in Figure . Upon a successful recognition and cleavage of the target DNA by Cas12a, the probe DNA immobilised on the electrode got effectively cleaved by the trans collateral activity of the enzyme. The corresponding change in the electrode's resistance could be subsequently recorded by EIS. Using this system, the authors reported an LOD of 3x10 -9 M. This work is opening the door for further developments of using the novel CRISPR/Cas system for biosensors development. More studies are, however, needed to assess the potential of this novel technology in POC application.