Retention time features capture the behavior of the analytes on the liquid chromatography (LC) column. Based on theoretical retention times predicted for the candidate peptides, the difference with the experimentally observed retention time (ΔRT) can be calculated, typically in terms of the absolute differences. Most rescoring pipelines incorporate retention time features (Table ). DeepRescore reported that while dropping ΔRT and SA led to reduced peptide identifications, only minor reductions in the number of identified peptides were observed when only ΔRT and SA were considered. This indicates the dominant contributions of these features. The interplay between ΔRT and SA was further highlighted by the fact that eliminating either of these features resulted in a loss of peptide identifications. This observation implies a complementary contribution from both features, which is expected as the LC behavior of a peptide is independent of its MS behavior.