Recent impressive developments in DNA nanotechnology have led to significant progress in sensing, imaging, and mechanical devices , , . As a result, there is an optimistic outlook that the creation of self-sufficient vehicles, capable of identifying issues within the body and taking appropriate action, may soon become feasible . Two DNA nanomachines detected pH changes in specific parts of cells using two different pathways, providing real-time monitoring of early endosomes and the trans-Golgi network. They worked independently and accurately captured the pH of their target organelles . The recent breakthroughs in DNA nanotechnology have enabled the realization of DNA nanorobots that can execute preprogrammed functions. By integrating sensory, controller, and actuation modules, these DNA nanorobots demonstrate the ability to execute diverse mechanical motions in response to specific stimuli. DNA nanorobots possess a wide range of functionalities, such as targeted drug delivery, precise biosensing, and the capacity to autonomously influence cellular behavior according to specific requirements . Researchers created an autonomous DNA nanorobot using DNA origami that can deliver payloads to tumors. Incorporating thrombin and a nucleolin-targeting aptamer, the nanorobot utilized a single entity that acted as both a targeting domain and a molecular trigger. It induced intravascular thrombosis and inhibited tumor growth in tumor-bearing mouse models. In both mice and miniature pigs, the nanorobot demonstrated a safe profile and did not elicit any significant immunological responses . DNA origami is being investigated as a platform to enable the development of in vivo interactive nanorobots and trigger the activation or deactivation of molecular payloads through logical outputs. After successful ex vivo experimentation, researchers advanced to using the DNA origami robots within live cockroaches to exert control over a molecule that selectively targets their cells .