In general, we were able to demonstrate that the addition of a methylated group does not change the photophysical properties of the modified 6-Aza Uridine. The theoretical calculations showed that the addition of a group does not notably affect the spin-orbit coupling values. The steady-state experiments supported by the calculations demonstrated that MTAU and TAU primarily emit photons through fluorescence, indicating that the S 1 excited states predominantly return to the ground state by a radiative decay pathway. A small fraction of the 1 pp* population is predicted to intersystem cross to the triplet manifold in both TAU and MTAU. We were also able to demonstrate that the addition of the methylated group prevents the tautomerization of the modified 6-Aza Uridine compound.