In this section, we present the preferential biding coefficients of cavity with urea and TMAO in waterurea, water-TMAO and water-urea-TMAO mixtures. The values of preferential binding coefficients of cavity are provided in Table . Urea molecules are always preferentially excluded from the solvation shells of neopentane cavities. The highly negative value of γ for urea indicates an excessive depletion urea from the solvation shells of cavity. Interestingly, TMAO molecules is also preferentially excluded from the solvation shells of cavity, however the magnitude of depletion is less compared to the urea depletion around cavity. In contrast to urea, it can be noticed that TMAO is preferentially excluded more in water-urea-TMAO solution.