It was also necessary to apply PSiFe onto the air/water interface using a special protocol adjusted from the literature. Nanoparticles were deposited onto the interface using a Hamiltontype syringe with the help of a glass rod. A clean glass rod was immersed in the center of the trough, and the sonicated PSiFe suspension was deposited dropwise onto the rod's unimmersed portion using a Hamilton syringe just above the water surface. After deposition, the rod was gently removed to facilitate the smooth spreading of PSiFe at the air-water interface.