Next, the impact of linker size is examined by using a shorter bidentate linear linker molecule compared to the previously employed one. Specifically, a dpe linker is utilized in place of the longer dpb linker. This substitution leads to the formation of Cu10-dpe CAM. The synthesis of Cu10-dpe CAM is carried out under reaction conditions identical to those used for the preparation of Cu6-dpb CAM, allowing for a direct comparison of the structural and functional effects of the different linker sizes. SCXRD analysis reveals that this Cu10-dpe CAM crystallizes in a monoclinic crystal system with a space group of P21/c (14). The core structure of the cluster consists of ten Cu(I) atoms, which are stabilized by six S t Bu -ligands and four CF3COO -ligands, along with four dpe linker molecules resemblance to the structural architecture of Cu10-dpb CAM. So, a similar redistribution of Cu(I) atoms occurs with the incorporation of an additional Cu4 sandwiched layer, despite using the same solvent and surface-protecting ligands as before, indicating that the change in linker molecule drives this structural variation. Anyway, the cluster forms an extended 2D square framework (Fig. ), with individual cluster nodes spaced 13.6553 Å apart within the plane. The dpe linker itself measures 9.641 Å in length, suggesting that the shorter dpe linker favors the formation of a larger cluster node compared to the longer linker molecules. However, we noted a specific difference in this CAM compared to the Cu10-dpb CAM regarding the connection of the cluster node. In this case, we observed an alternate orientation of the adjacent cluster nodes that was not present before. However, when a relatively larger, non-linear linker molecule dpt was introduced into the synthesis process following a similar reaction pathway, we again observed the formation of a similar Cu10 cluster node structure. Due to the non-linear nature of the linker, however, the resulting framework exhibited a wavy, two-dimensional network, unlike the more planar structures typically seen with shorter, linear linkers (Fig. ). Thus, both the shorter and longer than dpb linker produced a Cu10 cluster node, while the same reaction protocol is being used. This suggests that the determining factor for cluster node formation is not directly related to the length of the linker molecule, but rather to the lone-pair donating capabilities of the pyridine moieties within the linker. We measured the zeta potential values of the linkers and found that both the dpe (-13.9 mV) and dpt (-1.92 mV) linkers exhibited negative zeta potential values, in contrast to the dpb linker (2.06 mV), which shows a positive value at neutral pH medium (Fig. ). This indicates a variation in the electronic environment on their surfaces. We assume that the negative zeta potential values are due to the stronger lone-pair donating capabilities of the pyridine moieties in the dpe and dpt linkers, which facilitate the formation of larger aggregates of Cu(I) atoms, leading to differences in cluster size and structure. This nuanced interaction between linker properties, especially their electrondonating abilities, highlights a critical factor in determining the structure and stability of Cu(I) cluster nodes in these frameworks. To further elucidate the rearrangement of the Cu10 cluster node in the Cu10-dpb CAM when altering the solvent environment compared to the Cu6-dpb CAM, we propose that