Coupled GC/EAD analysis of pheromone gland extracts was conducted on a Varian 3800 GC equipped with a flame ionization detector (FID) and a splitless injector. The column effluent was split 1:1 between the FID and EAD apparatus. Antennal depolarization was detected using a high-resistance EAD Probe (Signal Interface Box, Type ID-02) and Intelligent Data Acquisition Controller (Type IDAC-02) (Syntech, Hilversum, The Netherlands). Antennae from 2 to 3-day-old males collected from Lincoln and Little River were excised at the base and attached to the silver electrodes housed in saline-filled glass electrodes using a micromanipulator (Narishige, Tokyo, Japan) to facilitate electrical connection. Up to five antennal preparations from each location were tested with different female extracts from the same location for GC/EAD analyses. A 30 m  0.25 mm internal diameter (ID)  0.25 µm VF5-MS capillary column (Factor Four, Varian Inc.) and a Y splitter (Alltech, Deerfield, IL) were used for the analysis. The oven temperature was programmed to increase from 80°C (held for 1 min) to 240°C at 10 °C/min. Helium was used as the carrier gas.