In short, when the degradation is limited (i.e. between 0.5 to 10 %), both SEC and MALDI-TOF analyses can be employed to confirm the occurrence of degradation. SEC can be used to quantify precisely the extent of degradation, while MALDI-TOF analysis can be used to identify the products formed, and in particular the end groups generated. Note that if the depolymerization medium is rich in salts, samples must be desalted before MALDI analysis, which is highly sensitive to the presence of ions. Conversely, when there is a need for high-throughput analysis, MALDI can be used in the first instance to rapidly observe degradation, without however being able to quantify it, before carrying out a quantitative SEC analysis on the most interesting samples. In the special case where depolymerization takes place in exo, HPLC can also be considered as a complement to these two techniques, in order to precisely identify the lower molecular weight products formed, provided they are available as analytical standards. If depolymerization exceeds 10 %, NMR and FT-IR can be added. Interestingly, the latter may be sufficient on its own initially to quantify degradation, if a calibration curve can be made beforehand. As this technique requires very little sample processing before analysis, and as we have succeeded in adapting it to high throughput, it could prove to be a very good approach for large-scale screening, particularly when studying microorganisms or enzymes. Finally, we generally recommend considering coupling at least one method for identifying reaction products with a method for quantifying the progress of depolymerization. MALDI-TOF is an effective approach for identifying each end group and, consequently, the various reaction intermediates, and can also be adapted for high-throughput screening. Coupled with the low sample quantity and solvent requirements, it is certainly an interesting first option, if the polymer can be analyzed in this way, as is the case with PBPAC. -Signal superposition is possible, especially when product diversity is high, which can make it impossible to identify reaction intermediates. HPLC 20 % * -Highly sensitive method in the case of an exo degradation, with a BPA detection limit of 6 µM; -Quantitative, and allows the identification of all degradation products as long as analytical standards are available.