The critical dose of {431} and {110} Bragg planes improve by a factor of 1.29 and 2.79, respectively, when the accelerating voltage is increased from 200 kV to 300 kV . Liang et al. emphasize the importance of selecting the right incident electron energy to balance image resolution, contrast, and beam-induced damage to organic two-dimensional (2D) materials . By testing different acceleration voltages (300 kV, 200 kV, 120 kV, and 80 kV), they found that lower acceleration voltages increase inelastic scattering and radiolysis damage, suggesting higher voltages are generally beneficial. However, 120 kV offered the best resolution and information coefficient within the critical dose, improving signal-to-noise and image contrast. Russo et al. also compared the radiation damage to bacteriorhodopsin and paraffin (C44H90) at 100 kV and 300 kV by observing the fading of diffraction spots . They found that radiation damage per incident electron is 1.57 times higher at 100 kV than at 300 kV. However, 100 kV offers 25% more useful information for most biological structures compared to 300 kV . It appears that higher accelerating voltage, such as 300 kV, improves critical dose ; however, within the critical dose limit, lower accelerating, such as 100 kV or 120 kV, provides better information coefficient, resolution, and image contrast .