The profile dataset is comprised of liver and brain tissue samples of the salmonid species Chinook (Oncorhynchus tshawytscha). This data has been made publicly available and can be obtained from the Metabolomics Workbench repository: ST004904. Fish husbandry and euthanasia were performed in accordance with the Standard Guide for Conducting Acute Toxicity Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians 1 (ASTM, 2014). These methods were approved by Oregon State University Institutional Animal Care and Use Committee (IACUC-2022-0260). Fish were housed in the Aquatic Animal Health Laboratory (AAHL) at Oregon State University (OSU). Fish were acclimated to AAHL holding conditions in 100 L constant flow-through tanks containing ambient well water (16°C) and constant oxygen supply. Fish were fed a commercial salmonid feed at a daily rate of 1% body weight daily during acclimation until the average weight of approximately 3.5 g was achieved as a target experimental weight, including the un-fed control group. Each tank included five fish replicates and each condition was repeated to collect three biological replicates. Fish were sacrificed using MS-222 (Tricaine mesylate powder) + 50 g/L bicarbonate. In this study, the measurements from the fed and un-fed fish were used. The un-fed fish were no longer fed 24 hour prior to the euthanasia. Fish liver and brain tissues were dissected, weighed, and flash frozen in aluminum foil packets using liquid nitrogen and stored at -80°C. Liver and brain tissue samples (10 mg) were aliquoted in 2 mL screw cap vials prefilled with 40 µL volume of 1.4 mm ceramic beads.