To further study the size, shape, and morphology of AP205 VLP, the SAXS data were analyzed with the generalized indirect Fourier transformation (GIFT) method (for details, see supporting information) The measured zeta potential in PBS of -8.8 ± 2.1 mV (see Figure ) was used to approximate the effective particle charge for the structure factor, S(q), model. The S(q) parameters are summarized in Table . The effective interaction radius and volume fraction were varied to find the best possible fit of the model to the experimental data, resulting in 18.3 ± 0.5 nm and 1.15 ± 0.07 %. The volume fraction shows good agreement with the experimental mass fraction of approximately 1 wt%. This model leads to a stable pair distance distribution function, p(r), showing that the virus particles were quasi-spherical with a diameter of around 30 nm, obtained from p(r) = 0, see Figure , which aligns reasonably well with the radius predicted by the S(q) model.