The EnzymeMap dataset version 2.0 (along with the remapped KEGG and MetaCyc files) is available via Zenodo at doi.org/10.5281/zenodo.7841781 as a machine-and humanreadable CSV file containing the EC number, the original reaction text, mapped and unmapped reaction SMILES, as well as further metadata as described in the methods section. The code to reproduce all processing steps from a raw BRENDA entry to a validated, mapped reaction is available as easy-touse Python package at github.com/hesther/enzymemap. The repository furthermore contains scripts for the retrosynthesis and regioselectivity models. Instructions to retrain the transformer model are available in Ref. 43 and were used as provided.