During the sprouting process under salinity stress, pea seeds were exposed to stress induced by salt (NaCl). The seeds were placed on a seedling tray, with half of each seed submerged in water containing NaCl at concentrations of 0, 25, 50, 75, or 100 mM. The germination process lasted for 36 to 168 hours. As for the stress induced by alkali, Na2CO3 was used at concentrations of 1.0, 2.0, 5.0, and 10.0 mM. Drought stress was simulated using polyethylene glycol (PEG-2000) at concentrations of 0.5, 1.0, 2.0, and 5.0%. To induce heavy metal ion stress, Pb 2+ was introduced at concentrations of 0.25, 0.5, 1.0, and 2.0 mM. Additionally, the effect of Bacillus subtilis (B. subtilis) in mitigating stress of each group was tested at concentrations of 1.0, 2.5, and 5.0×10 8 CFU mL -1 . After sprouting for 36, 72, 120, or 168 hours, six pea sprouts from each seedling tray were treated with TBPBB (40 µM in water medium) for 1 hour in darkness before fluorescence imaging.