The selectivity and mixture selectivity of the histamine detection were carried out to prove its potential for real-sample detection. For the OA nanozyme-colorimetric sensing systems validation of selective histamine detection, several biological, agricultural, and food molecules are prepared, and conducted the colorimetric assay parallelly along with the controls (with positive/negative(blank) control) and the histamine solution (all concentration at least 1mgmL -1 ).