1. Hydrogen bonding Watson-Crick base-pairing to design and assemble synthetic DNA strands into complex structures with high precision and specificity. One of the effective bottom-up fabrication techniques based on this type of interaction, in DNA nanotechnology, named DNA origami, which is raster-filling a pattern by folding a long ssDNA called 'scaffold' by binding with some short strands, called 'staple', via baseparing to make the whole pattern stable. This well-defined methodology, which can be assisted by computational designation, has provided the possibility of precisely knitting various patterns and structures from one-dimensional (1D) to threedimensional (3D) or even hierarchical assemblies , , , . The DNA tile-based method, which is also based on hydrogen bonding interaction derived from immobile branched DNA junctions , is the other approach in synthesizing DNA nanostructures , and can also be used in crystals and periodic assemblies with characterization and programming of designed arbitrary shapes and geometries , , , . 2. Stacking interactions between adjacent base pairs in the DNA helix, mediated by der Waals forces, contributes to the overall stability and rigidity of the double helix structure and thus stabilizes the assembly of DNA nanostructures, which is effectively applied in designing dynamic nanodevices , .