Unphosphorylated G159D troponin has a lower mean A/B helix angle than unphosphorylated wild-type and higher probability of being in the range of angles defined as the open state. The mean hinge angle is similar to phosphorylated wild-type troponin but the distribution is narrower. In general, unphosphorylated G159D resembles phosphorylated wild-type more than unphosphorylated wild-type.