Researchers developed a portable microfluidic device that combines filter paper strips with screen-printed carbon electrodes (SPCE) for the direct detection of Pb(II) and Cd(II) in aqueous samples. The device was tested for its analytical performance for the direct quantification of multiple analytes without pretreatment. The square wave anodic stripping voltammetric (SWASV) signal was significantly enhanced and displayed excellent analytical performance for Pb(II) and Cd(II) detection from 0 to 100 ppb with low limits of detection of 2.0 and 2.3 ppb, respectively. The proposed electrochemical devices exhibited good selectivity and stability for analyses of real samples of gas dissolved salty soda water and ground water with physical contamination. This paper-based microfluidic device is simple, low-cost, easy-to-fabricate, and portable, making it an ideal candidate for point-of-care applications in environmental monitoring, public health, and food safety.