). To test our strategy, we designed 4 a prototypical origami triangle 3 held together by 261 staples and comprised of three independently folding sectors (Fig ). We extended both the 3′ and 5′ ends of 258 staple strands with the sequences described above and positioned hairpins on either side of the origami. We folded the 258HP nanostructure (258HP) using a 8064-nt M13 single-stranded DNA scaffold Taken together, these results show that addition of hairpins at the staple-staple junctions is compatible with origami folding and might also rigidify the nanostructure.