Here, we report results from two workflows for mapping the proteoform landscape of solid tissues and present the first iteration with five commonly studied human tissues (heart, lung, kidney, small intestines, and spleen). Initially, the extracted proteoforms were pre-fractionated using Gel-Eluted Liquid Fraction Entrapment Electrophoresis (GELFrEE), followed by subsequent CZE-MS and nano RPLC-MS analysis. This study contributes 7,373 proteoforms to the Human Proteoform Atlas (HPfA) a FAIR knowledgebase that now contains approximately 60,000 unique proteoforms linked to their biological context.