The presence of silanol sites can vary the reference point of the measured signal leading to hampering the detection of the biomolecule's actual signal. Thus, with an increase in the population of the silanol sites in presence of IPP, the shifts towards the left-hand side, as shown for 2<pHpzc<14 in Fig. . The minima and maxima points of ⁄ clarifies the transition or saturation of charges with the increase in silanol sites [Fig. ]. The charge saturation/transition can be observed in sensor output between the inflection points for silanol sites close to 50% whereas for the silanol population close to 10% or 90% can be differentiated by the change in drain current amplitude across the pH range. Similarly, to the potential, the CT minima shifts towards lower pH values with the increase of silanol mimicking the pHpzc [Fig. ].