Samples of PEI-M MMT / Polysaccharide-M MMT/ Polysaccharide-MNPs/L-M MMT hybrid and inorganic (M MMT) of known weights (20 mg) were placed in a glass vial containing 15 ml of phosphate buffer at pH 6.5 at ambient temperature for slow release studies of loaded Chp loaded NCs. The set-up was gently shaken and the was weighed, pH was measured (Table in SI) and 1 ml of the liquid was removed for examination and supplemented with a fresh 1 ml of the medium to preserve the sink state. After syringe-filtering the aliquot, a UV-Vis spectrometer was used to investigate the release and correlate the concentration emitted with the Chp calibration map.