The non-covalent interactions with active site residues in this complex largely align with the previously reported 5-methyl-2'-deoxyuridine complex (PDB: 1F8Y). The exocyclic amine of 8 forms a non-covalent interaction with the C-terminus of Y157, whereas the endocyclic N3 forms a hydrogen bond with the side chain of Q46. Finally, hydrogen bond interactions were observed between O2 of 8 and both Q46 and D72 side chains.