Seed cultures were inoculated from frozen stock in 25mL media A and grown to stationary phase, then subinoculated into 40mL media A at an OD730 of 1.0 to synchronize growth and grown overnight. PBRs were inoculated to an OD730 of 0.1 and induced after 24 hours with 1mM IPTG. At 24 hours, phosphate feeding was achieved with constant flow of 200mM K2HPO4 through a 3-channel peristaltic pump and feed rates were monitored by measuring reduction in mass over time.