Theoretical and experimental work cited above show the influence of several factors and parameters on DNA denaturation in the presence and absence of different solvents. However, the work will continue to develop an equation connecting the rate and degree of DNA denaturation with the following interrelated parameters: the structure and state of DNA, the composition of surrounding media (solvents, buffers, and pH), and temperature. Such information helps to select the proper (co)solvent or predict the required structure and concentration of an additive to a solution (buffer) sufficient for good DNA denaturation or double-stranded DNA stabilization at a given temperature.