Rheological properties and curing kinetics of different HA-based hydrogels were determined using a rotation rheometer (DHR3, TA Instruments, USA) equipped with a 12 mm parallel plate geometry as upper plate and a transparent lower plate connected to a UV/Vis illumination source (Omnicure, Series 1500) with a 400-500 nm filter to allow in situ curing of hydrogels at room temperature. For each polymer HA-NB, HA-MA and HA-AE, hydrogel precursor solutions were prepared as above. An aliquot was loaded onto the transparent lower plate of the rheometer using a micropipette and the upper parallel plate was lowered to a gap size of 300 µm. The sample was surrounded with silicone oil to prevent evaporation and covered with a metal cover to avoid disturbance during the measurements. Strain sweeps (0.1 to 100% strain at frequency = 2 Hz) and frequency sweeps (0.01 to 100 Hz at strain = 1%) were performed on previously crosslinked hydrogels to determine the linear viscoelastic region. Time sweeps where then carried out in the determined parameters. Storage and loss moduli were recorded over time using 1% applied strain and 2 Hz frequency. For continuous curing measurements, the samples were irradiated with 400-500 nm light (15 mW cm -2 ) from the 60-s time point onwards using TRIOS software to automatically start the illumination. For sequential curing measurements, the same light source was switched on and off manually. To compare crosslinking kinetics of the different HA derivatives, the gel point tgel where G' = G" was compared. Stiffness of the hydrogels were determined by comparing G' after 20 min of irradiation.