GUVs are formed by hydrating the lipid films, the lipid molecules due to their amphipathic nature self-assemble upon addition of water such that the hydrophobic tails are shielded, and hydrophilic heads extend outside forming the vesicle. In 1986, Angelova and Dimitrov improved the protocol by applying an alternating current (AC) electric field during the swelling process which significantly improved vesicle yield, monodispersity, and unilamellarity. The electroformation protocol is now well established as researchers have worked around the application of different AC frequencies, amplitudes, temperature parameters, and electrodes (ITO/Pt) to get GUVs in required conditions.