The serum of COVID-19 patients contained proteins such as ORF1ab, ORF3a, ORF8, S, and N. Antibody detection methods for these proteins are also used to confirm the infection with SARS-COV-2. It demonstrates that viral proteins can also be secreted into the serum by infected cells. ORF3a, and S protein must be secreted into serum to attack red blood cells. The V-type pathway was initially described as the IgA1 protease. It ensures that the proteins secreted through the outer membrane are completely contained within the secreted protein . An autotransporter is a protein that is secreted in this manner(93) (Autotransporter). Some proteins autocatalyze cleavage after the Autotransporter domain induces itself export, while others dose not . Some eukaryotic viruses use the asparagine-aspartate self-cleavage mechanism during capsid maturation . The SARS-COV-2 virus protein may also use an autocrine mechanism similar to this.