Next, we investigated the effects of the top-ranked candidates, i.e., 5a, 5e, 5i, and 5m, on interaction with a specific subtype of NMDAR associated with neurodegeneration, namely the GluN1/GluN2B receptor . The rationale for testing the compounds for their affinity toward NMDAR is based on the well-established interaction of tacrine-based compounds with these receptors .