Infection with any one of three wheat mosaic viruses-wheat streak mosaic virus (WSMV), wheat streak mosaic virus (TriMV), or highland wheat mosaic virus (HPWMoV)-leads to the complex disease known as wheat mosaic disease . Wheat streak mosaic virus is a tritimovirus belonging to the family Potyviridae, which includes the very large genus Potyvirus, similar to tobacco mosaic virus . The wheat streak mosaic virus (WSMV) is a member of the family Potatoviridae, genus Tritimovirus, and species Tritimovirus. WSMV is a positive-sense, single-stranded RNA virus . Even though seed transmission of wheat mosaic virus is relatively infrequent, the wheat curl mite (WCM, Aceria tosichella) is an important vector for this virus. While both wheat and N. benthamiana are susceptible to CWMV infections, inoculation is most effective at a temperature of 17 °C [7] . Plants with the disease are short and have yellow rings of parallel, broken stripes on their leaves .