The first children libraries obtained from evolutionary-fitting the brodifacoum-rat VKORC1 showed that the brodifacoum-parent predicted more and higher children affinities (lower docking-scores) (Figure ) than any of the two other parent fragments derived from brodifacoum containing either its tail or hydroxycoumarin head moieties (Figure , DWR4, DWR5, respectively). Additional evolutionary-fittings were performed therefore, starting from the whole brodifacoum molecule as the parent to generate brodifacoum-children.