Biotin-JVZ007. To 62.5 µL of a 96 µM solution of α-oxo aldehyde JVZ007 in pH 7.5 buffer (40 mM sodium phosphate) was added 25 equivalents of biotinylated SCALP-donor probe S6 via the delivery of 37.5 µL of a 4 mM solution of S6 in water. This yielded 100 µL of a solution containing 60 µM α-oxo aldehyde JVZ007 and 1.5 mM S6 in pH 7.5 buffer (25 mM sodium phosphate). The resultant solution was then incubated at 37 °C in darkness for 2 hours, after which time the biotin-JVZ007 sample was diluted to a volume of 1 mL and dialysed at 4 °C for 18 hours into pH 7.5 buffer (25 mM sodium phosphate) using 6-8 kDa dialysis tubing (SpectraPor®). The sample was then desalted into HPLC grade water using a PD MiniTrap™ G-25 desalting column (Cytiva), flash-frozen using liquid nitrogen, and lyophilised.