Several POP6 lipids remain in close proximity to the protein for several microseconds (Figure ), and lipid exchange can be observed at the four PI(4,5)P2 sites (Figure ). The fractional occupancy analysis shows that POP6 lipids occupy the four PI(4,5)P2 headgroup sites in the tetramer, with occupancy maps confined around residues that are known to interact with PI(4,5)P2 (Figures and).