Although ELISA is more sensitive than immunoaffinity chromatography (approximately 10 × 10 8 /mL), it requires cumbersome multistep preparation and a relatively long time for antibody binding and enzymatic reactions. Other highly-sensitive detection apparatuses can be used to directly detect captured antigens on an antibody-coated solid substrate. For example, antigens adsorbed on the affinity microbeads can be detected using fluorescencebased flow cytometry (FCM) after fluorescence labeling. It is also possible to detect antigen adsorption without labeling using mass-based detection methods such as the quartz crystal microbalance (QCM) and the surface plasmon resonance (SPR). However, these apparatuses are only available in well-funded central laboratories. There is still a strong demand to establish a simple, fast, and sensitive target-antigen detection technique for pointof-care (PoC) applications to be used in common medical facilities and laboratories.