The pooled Nigerian urine sample, including MS2 data measured in negative and positive ionization mode with iterative exclusion lists (n=4), was used to screen for potential additional xenobiotics not covered by the targeted evaluation using authentic reference standards. The suspect screening was performed in R, applying the patRoon package . Solvent process blanks (only in the corresponding polarity) were defined as blank measurements. The raw data files were converted to mzML-files and centroided with ProteoWizard . For peak picking and grouping, the "openms" algorithm was set with the following parameters: noise threshold: 4E3, chromFWHM: 3, minFWHM: 1, maxFWHM: 30, chromSNR: 5 and mzPPM: 3. Only features with a minimum absolute feature intensity of 3E5, a minimum feature intensity above blank of 10, present in at least 60% of replicates, were kept and blank analyses were removed after this step. A suspect list from the ENTACT trial based on the EPA's ToxCast library, including > 4000 substances, was adopted for this experiment. Analytes which had already been included in the targeted list were removed to avoid redundancy. For the suspect screening, an m/z window of 0.002 was set. MS peak list data were extracted with the mzr-algorithm (precursor m/z window: 0.5) and filtered (relative intensity threshold: 0.02, top 10 MS/MS peaks