allyloxy)methyl)-quinolone 1a-2a, 4a-6a, and 8a-10a with high conversions and excellent selectivities. Reactions to produce 3a, 7a and 11a are performed with EnT1.2, which gave higher levels of selectivity compared with EnT1.3. With N-methyl quinolone 12, reactions with EnT1.3 proceed with modest selectivity, which can be improved with a rational Y121F mutation. Reaction conversions are the mean of biotransformations performed in triplicate. The absolute stereochemistry of products 2a-12a were assigned by analogy to the product (-)-1a formed by EnT1.3. Reaction conditions for the synthesis of 1a-12a are presented in SI Table .