By applying the VIEC approach to each group of DLL and SLL, the dynamics of release from the vesicles in each group were statistically analysed (2182 spikes for DLL and 2301 spikes for SLL, 10 traces for each, see details in Supporting Information). Typical amperometry traces for DLL and SLL and the spike analysis parameters are shown in Figure and Figure , respectively. The results reveal that the average quantities of both monoamine molecules within each individual liposome and the height of the spike (I max ) of both groups are similar, while a significant increase in t half and also a relative decrease in fall time (t fall ) is observed in the SLL compared to the DLL. Typical spikes (in the form of logarithmic dimensionless current) for each of DLL and SLL are shown to the right of Figure . The decay of the DLL spike has been fit to a rapid decay (~1 ms) and subsequent slow long decay (~10 ms), and it is better fit to a DblExp decay function than a SigExp function, whereas the spike of SLL fits better to only a a SigExp function. We further compared the number of spikes having DblExp vs. SigExp decay for all spikes from DLL and SLL. Indeed, when the ratio of χ 2 (single)/χ 2 (double)>1 set as a cutoff, the percentage of spikes that have a better fit to DblExp decay in the DLL group (~70%) is higher than for the SLL group (~60%). This result shows that dopamine release from the liposomes is more complex and generally slower than 5-HT, which might result from the slower desorption of DA from membrane lipids (liposomes have no dense core).