Proteases are a class of enzymes targeting peptide bonds, which are often released by bacteria in the ESKAPEE group. Protein-based hydrogels are therefore good candidates for protease susceptible sensing systems. Proteases are organized into different classes, namely aspartic, glutamic, metallo, cysteine, serine, asparagine, and threonine proteases, based on their different catalytic sites. They have also been grouped according to similarities in the active sites and substrate affinities, for example in the MEROPS database. Different types of collagen are known to be degraded by bacterial collagenolytic proteases such as metalloproteases of the M9 family, as well as serine proteases from S1, S8 and S53 families. Among the common pathogenic bacteria found in wound infections, S. aureus, P.