In the PCR room, the instrument used to run the test was called the Bio Rad CFX96 Real-time PCR detection. The CFX96 used the following fluorescents to detect the following genes in the sample: FAM to detect S gene, HEX to detect ORF8 gene, Texas Red to detect N gene, and Cy5 to detect IC (RNase P) gene. The PCR system temperature conditions are as shown in Table .