However, it should be noted that the number of intramolecular hydrogen bonds is generally very low in all cases. To further elucidate hydrogen bonding behavior in our systems, we computed the hydrogen bond ACFs from overall direct hydrogen bonding (intra-and intermolecular combined); the results for this analysis can be found in Figure . Although the tail-corrected hydrogen bond ACFs are almost indistinguishable between MMG-1 and MMG-6, the decay profiles display differences between the systems. The initial decay of the hydrogen bond ACFs is overall fastest with the single bilayer system, indicating that the double bilayer configurations have more stable hydrogen bonds. Contrary to the other membrane configurations studied here, all MMG molecules are in contact with water in the single bilayer system. With order parameters and the tail tilt showing the most considerable differences in the single bilayer system (Figures and), we investigated if these properties could be linked to differences in headgroup hydration.