Among these methods, non-hydrogenative parahydrogen induced polarization (nh-PHIP, Scheme 1) has been used to detect analytes in urine, whiskey and coffee extract, making it a promising tool for the quantitative analysis of complex biological mixtures. However, previous studies have also highlighted the challenges in achieving compatibility between the nh-PHIP hyperpolarization system and the inherently complex biofluid matrix. In the case of urine, solid phase extraction or ammonia stripping was required to remove biofluid components that interfere with the hyperpolarization process. These sample pretreatment methods allowed the detection of N-heterocyclic metabolites and oligopeptides in urine. On the other hand, amino acids could be detected with nh-PHIP without pretreatment due to their relatively higher concentration in urine and the slightly modified iridium catalyst used in the process.