As expected, each sensor produced a measurable current signal for its specific antibody with minimal crossreactivity (Figure ). antibodies. Experiments were performed at 25 °C in a 20 μL solution containing each of the GOx-DNA duplexes (100 nM each), the antigen-conjugated strands (160 nM each), the EGFR-DNA strand (180 nM) and the indicated antibodies (100 nM). After addition of the antibodies, the solution was allowed to react at 25 °C for 30 minutes and then transferred to the electrode surface for the amplification reaction (see legend of Figure for experimental conditions of the amplification).