8.5.1. Description of the isolated compounds. atmosphere for 70 h. 10 µl resazurin solution (resazurin, 12.5 mg in 100 mL double-distilled water) was then added to each well and incubation continued for a further 2-4 hours . Then the plates were read with a Spectramax Gemini XS microplate fluorometer (Molecular Devices Corporation) using an excitation wavelength of 536 nm and an emission wavelength of 588 nm. The data were evaluated in Excel. For each test concentration, the percent growth inhibition was calculated in comparison with an untreated control and melarsoprol at 0.07 μg/mL was included as positive control.