Screening of ethyl acetate extract derived from rice culture of A. violaceus was conducted using hyphenated HPLC-DAD/MS revealed several protonated molecules with one at 266 Dalton as the main product. The extract was subjected to successive chromatographic separation procedures to isolate seven new compounds. In addition, the structure of 1 was previously reported by Nozoe et al. in a Japanese patent (1997). However, no detailed spectral data were reported.