Although mass spectrometry imaging is recognized as a potent analyWcal technique that can idenWfy and map "hundreds of molecules in a single experiment" , we must remember that imaged molecules have to be able to ionize. At the beginning of our study, we aimed to compare the NEDC matrix and glucose derivaWzaWon with betaine aldehyde. Then, considering the ease of the procedure and the features of the spectra, wanted to choose the be]er and more straigh}orward technique.