With the recent availability of AlphaFold3 and other models, AlphaFold2 remains the best choice as a hypothesis generator for our task. Most importantly, AlphaFold2 allows for Using the best pLDDT prediction from the full MSA run as the reference structure, all 1305 structures had a C α RMSD of < 6.0 Å (Figure ). Given this low RMSD, all structures were retained. We used all pairwise distances between the core domain and the NMP domain (residues 30-59) and between the core domain and the LID domain (residues 122-159) as the initial CVs. The core domain was defined as anywhere other than the NMP and LID domain. From each group, the top 200 distances with the highest coefficient of variance were selected, resulting in a total of 400 pairwise distances. The structures were clustered in this 400-dimensional space using regularspace clustering, with a distance threshold of 3 Å. Ultimately, 7 structures were selected, and MD simulations were run for 100 ns from each.