where the sum runs over T=250 trajectory frames for each repeat separately with coordinate vector r t being either the Cα positions or the backbone bead positions and r 0 is the coordinate vector of the starting position. For both the RMSF and RMSD calculations, each monomer frame was aligned to the starting crystal structure of the monomer (this is required to determine the mean coordinate vector for the RMSF calculation, so we chose the starting structure for consistency across all test systems).