Table captures the AMD (determined by SE) and the thickness (h) (determined by QCM-D) of the glycopolymer brushes with the most relevant target glycans (i.e., Gb3 for STxB and Le x for CTB). The brush thicknesses (15 to 20 nm) exceed the hydrodynamic diameter of the toxin B5 molecules (~5.5 nm) by several fold, indicating that the toxin can fully immerse and will experience a three-dimensional glycan environment within the brush. The grafting density (Γ) was here assumed to equal the surface-density of available biotin binding sites on the SAv monolayer (as determined by SE; Figures ). From the AMD and Γ, the number average molar mass (Mn,anchored) of glycopolymers was determined. It is notable that the average masses of the surface-anchored glycopolymers (Table ) are lower than the corresponding average masses of the glycopolymers in solution (as determined by SEC-MALS; Figure ). Most likely, this is due to the process of surfacegrafting preferentially selecting smaller polymer chains, as reported earlier. Considering in addition the degree of substitution of the glycopolymers with pendant target glycans (Figure ), the average contour length of the HA backbone (Lc,anchored) was obtained. That the brush thickness is comparable to or only marginally smaller than the contour length (Table ) implies that the glycopolymer chains are almost fully stretched in the brush environment.