Simulations, performed over a 50 ns timescale (Figure ), showed that the calculated RMSD values for both BRD4(1) WT and BRD4(1) L94C were comparable, with deviations stable and remaining below 2.5 Å for BRD4(1) L94C , after equilibrium had been reached. This result suggested that the L94C mutation would not significantly disrupt the stability or structure of the protein.