where F v (0) is nominally the INDUS free energy (Eq. ( )) associated with no water being present within the defined probe volume, and normalization by Ñv 0 (the average number of water molecules in v under equilibrium/unbiased conditions) permits more facile comparison across polymers. In other words, β∆f σ is intensive according to a system-specific, equilibrium reference. We note that INDUS calculations can result in irreversible system behavior for large probe volumes. We find that flexible, amorphous surfaces, can present hysteresis in calculation of Eq. ( ), depending on whether it is performed in the context of de-or re-hydration (see Supporting Information, Section S7). Therefore, our analysis and use of Eq. ( ) is restricted to removing water from the probe volume, and a consistent treatment enables us to use the calculation as a hydrophobicity metric, even if it may not rigorously correspond to a free energy.