Another cost-effective, highly sensitive, and specific paper-based microfluidic platform has been developed for rapid and multiplexed detection of important biomarkers in various body fluids, such as urine, saliva, serum, and whole blood. , The platform comprises five individual sensing channels with various functionalities that require only a small sample size, which is split into aliquots by the built-in paper microfluidics. To functionalize the various bioreceptors, wax barriers and "paper bridges" were used in an easy and low-cost manner. Pyrene carboxylic acidmodified single-walled carbon nanotubes (PCA/SWNTs) were deposited by quantitative inkjet printing with optimal 3-dimensional semiconductor density on a paper substrate. Multiple antibodies were immobilized onto the SWNTs surface for highly sensitive and specific field-effect transistor (FET)/chemiresistor (CR) biosensors. The sensing platform was optimized to achieve high sensitivities and specificities towards the target biomarker proteins, including human serum albumin (HSA) and human immunoglobulin G (HIgG), and achieved an ultralow detectable concentration of HSA and HIgG at 1.5 pM. Additionally, origami folding was used to simplify the fabrication process further. The platform is cost-effective, semi-automated, and user-friendly, and it envisions the potential for a point-of-care device with full analysis for practical diagnostics in an ASSURED (Affordable, Sensitive, Specific, User-friendly, Rapid and robust, Equipment-free and Deliverable to end-users) fashion for quick testing of targets of interest. The biosensing platform is expected to promote the future development and adoption of FET/CRs on paper substrates not only in resource-limited diagnostic applications such as ASSURED devices but also in other sensing scenarios, including on-site environmental monitoring, rapid detection of pathogens, and quick examinations of forensic samples.