A 0.1000 g of Betamethasone 17-Valerate standard was dissolved in HPLC grade ethanol and diluted to 100 mL with the same solvent. The solution was then diluted by 2-fold dilution and then 10-fold dilution respectively. It was run at a range of 300 to 200 nm. The wavelength for analysis of samples was chosen at 240nm because the figure 1 below of betamethasone 17velarate spectrum shows maximum absorption at 240nm.