Variation of 𝑁 loops at a fixed AL/RL ratio has a much more modest impact, with a larger number of loops leading to modest improvements in terms of hit scaffolds found (167±7 for 8 cycles vs 140±13 for 1 cycle) (Figure ) with unchanged hit diversity (0.19±0.003 vs 0.208±0.019) (Figure ) Following systematic experimentation of the RL-AL parameters 𝑁 acquired , 𝑁 batch , and 𝑁 loops , it was determined that a synergistic benefit is not achieved by simultaneously decreasing 𝑁 acquired to its lowest extreme (32) and increasing 𝑁 batch to its highest extreme (2048). While individually increasing both parameters enhances hit efficiency, 𝑁 acquired of 64 averages the greatest yield when considering all conditions, outperforming values of 32 or 128 after 30 000 oracle calls (Figure ). The relative diversity of each condition is largely unaffected by the selected settings. The highest average Tanimoto similarity was recorded as 0.277±0.026, with the lowest at 0.23±0.018. Detailed results for each condition can be found in