Based on the above-mentioned information, it is evident that enzymes are widely acknowledged for their catalytic efficiency and selectivity in chemical synthesis. However, their industrial application is often hindered by challenges related to scalability and sustainability. One significant challenge in enzyme-based reactions, particularly in polycondensation reactions for polyester synthesis using enzymes like CAL-B, arises from the presence and selection of solvent. Despite the common use of buffered water as a solvent in many enzymatic reactions, CAL-B's sensitivity to aqueous environments poses limitations, impacting its efficacy in polycondensation. Consequently, achieving optimal enzyme activity and reaction efficiency often demands strictly anhydrous conditions, necessitating the use of organic solvents. This challenge not only carries environmental implications but also contributes to energy consumption and costs due to the purification and precipitation steps involved in obtaining the final product. Even if the solvent is recovered after synthesis, additional energy is required. Therefore, there is a necessity to explore new, more sustainable approaches that can simultaneously result in a lower Environmental factor (E-factor). E-factor is a simple, yet reliable measure used to estimate the efficiency of production by comparing the amount of waste generated to the quantity of product created .