The mushroom and plant samples were purchased from local stores (Table .3). After acquisition, each sample was freeze-dried, and approximately 1 g was ground to a fine powder in a mortar and pestle. Then, roughly 50 mg was weighed into a 2 mL micro reaction tube (Table .3), and 500 µL extraction solvent (water:acetonitrile:methanol, 1:1:1, with 1% v/v formic acid) was added. The sample was briefly vortexed, placed in a thermoshaker (1 h, 75°C, 500 rpm), and centrifuged (10 min, 22°C, 18000 x g). The supernatant was subsequently transferred to an amber 1.5 mL LC vial. The extraction procedure was repeated a second time, and the supernatants combined. For the untargeted workflow, approximately 11 mg of Moringa leaves were extracted with 300 µL of extraction solvent since a higher dilution was needed as this sample contained elevated levels of polyphenols compared to the other organic material. The samples were prepared independently a second time for the targeted measurements (Table .3), with a 7-point calibration curve in neat solvent (Table .2). Two additional samples (50.5 and 50.1 mg, respectively) of white button mushroom were prepared and spiked with the reference standards (spike matrix-matched, Table .2) to estimate extraction efficiencies and signal suppression and enhancement (SSE) effects.