As shown in Figure (b), AMUSET-TICA accurately predicts all four slowest ITS for the folding of the FIP35 WW domain, which agree well with those obtained from a reference 1000-state MSM (referred to as the "ground truth"). Notably, AMUSET-TICA only requires a lag time as short as ~40 ns to correctly identify the timescales of all four slowest CVs. In contrast, tICA requires a lag time of 100 ns to identify CV1, CV3, and CV4 and fails to correctly predict the ITS of CV2 for the range of lag time (up to 100 ns) that has been tested (green lines in