Next, we initiated the system using different thiol triggers. We focused on biologically relevant thiol-analytes comprised of various sizes, including glutathione (SH-GSH), the protein bovine serum albumin (SH-BSA) and thiol functionalized DNA (SH-DNA) with concentrations of 132, 1.32 and 0.132 µM, respectively. Importantly, each SH-trigger (SH-BSA 37 , SH-GSH and SH-DNA) contains one thiol equivalent per molecule. Note that SH-BSA has additional disulfide bridges, which account for an additional 27% disulfides in the hydrogel system. We hypothesized that variations in the concentration of SH-trigger would lead to a differential selfpropagation speed and signal amplification rate which could be observed by the naked eye.