With the development of high-speed, high-resolution techniques, the shape and surface morphology of nano-sized colloidal systems can be visualized using a variety of electron microscopic techniques, such as the surface electron microscope (SEM), the transmission electron microscope (TEM), and the atomic force microscope (AFM). Each of these techniques has varying degrees of applicability to the characterization of the colloidal system. Among these techniques, TEM analysis is most commonly used to visualize lipid shell structure. The spherical nature of the particles can be visualized using both TEM and AFM techniques. In the morphological evaluation of CSHN systems, SEM analysis is not always a viable alternative because it deforms the nanoparticle structure due to the high vacuum applied during the imaging process.