Nevertheless, in 2019, Balusek et al observed variations in kinetic properties, particularly in diffusion constants when comparing 2 fs and 4 fs timesteps with HMR across various membrane-protein systems. , although for pure membrane systems, they found no significant differences in structural properties such as lipid order parameters, electron density profiles, and area per lipid. Recently, Sahil et al. investigated the mechanisms of protein-ligand recognition processes. Their research revealed that the 4 fs timestep with HMR required longer durations for ligands to identify proteins compared to regular MD simulations using a 2 fs timestep. These results suggest that while the use of a 4 fs timestep with the HMR technique can improve computational efficiency in various molecular dynamics applications it's important to note that the specific performance may depend on the system being studied.