Here we detail a facile single-step method that generates a sensitive and selective Cu(II) sensor on CFMs for utilization with FSCV. We perform anodic deposition of 1,4-diethynylbenzene (DEB), a symmetric compound with two ethynyl moieties, directly onto CFMs. The primary inspiration for this work is from Geiger and colleagues , who demonstrated that ethynyl functional groups can be anodically deposited on glassy carbon electrodes with complete surface coverage (and even multilayer coverage) in a controlled manner. We demonstrate here that we can anodically deposit ethynyl-containing compound like DEB onto CFM surface in a similar fashion. Moreover, the covalent attachment of DEB improves both sensitivity and selectivity of CFMs to Cu(II). This approach bypasses the need for a protection step and an ionophore step and reproducibly generates Cu(II)-selective biosensors in a more facile fashion. The long-term goal is to use these Cu(II)-selective biosensors to measure Cu(II) levels in cell and rodent models of neurodegeneration to elucidate the role of Cu(II) levels in the etiology of AD and other neurodegenerative disorders.