In this work, we developed and optimized a μCP protocol using industrial risk assessment tools. The use of a lubricant-infused surface provided a significant advantage by minimizing nonspecific attachment, while incorporating a crosslinker enhanced the stability of biomolecule immobilization through covalent bonding. We established an optimized bioink ratio of 50:50 between crosslinker and biorecognition molecule. It was also determined that 0.25% glycerol is the most effective additive in reducing the highly common coffee ring effect, which significantly improved the SNRs of our printed lubricant-infused microarrays. Finally, environmental factors, such as temperature, relative humidity and force application were all optimized, at 20℃, 60% relative humidity and 32.67kPa, respectively.