Fungi, a type of eukaryotes, are pathogenic to humans . It is reported that fungi-related infectious diseases have been emerging as an important public health problem . Evaluation of fungal viability and antifungal susceptibility was considered as key factors for the inspection and treatment of fungi-related infection in clinic . Previous methods widely used for fungal viability test are time-consuming and labor-intensive, involving counting the colonyforming unites (CFUs) after incubation, and using methylene blue to stain and count dead cells . In comparison, fluorescence imaging offers a more rapid and accurate method for fungi detection with the privileges of easier operation and higher sensitivity . In recent years, AIEgens that could stain fungi have been widely developed . An AIE-active probe DPASI has been designed by Zheng's group for sensing fungal viability (Fig. ) . This molecule is water-dispersible and could selectively stain dead C. albicans in a wash-free manner within 5 minutes. With the help of DPASI, live and dead C. albicans could be easily differentiated in fluorescence channel (Fig. ). The rapid imaging of dead C. Candida cells with impaired cell wall could be ascribed to the efficient binding of DPASI with mitochondria.