The newly generated spectral library in positive ion mode for the MCEBIO library was imported into mzmine and reprocessed to a feature list. Only MS 2 and pseudo-MS 2 spectra were used, resulting in 48,069 spectra, to reduce data complexity and the limited use of most tools for MS 2 spectra. The feature list annotation was exported as a .csv retaining the original information, e.g., compound name and adduct for later comparison. Furthermore, the list was exported with the mzmine module named molecular networking files in an .mgf data format, compatible with running feature-based molecular networking (FBMN) utilizing the Global Natural Products Social Molecular Networking (GNPS) infrastructure. The parameters for FBMN were set to precursor ion and fragment ion mass tolerances of 0.02 Da, a minimum pairs cosine value (min. pairs cos.) of 0.7 (minimum cosine score necessary to connect to experimental MS/MS spectra), a network TopK value of 1000, a minimum number of matched fragment ions of 4, maximum connected component size of 0, and a maximum shift between precursors of 500 Da.