It is worth noting that the bias potential from the WTMetaD-IB AL can be used to speed up the free-energy WTMetaD simulation. While the bias potential from AL is not directly proportional to the actual free energy, it might serve as a good starting point for the WTMetaD. The inherited bias generated after the 16 th iteration from WTMetaD-IB AL was employed as the starting bias in a WTMetaD simulation, referred to as WTMetaD+inherited bias (WTMetaD+IB) simulation. This accelerated convergence while maintaining the accuracy of WTMetaD started from the unbiased surface (Fig. ). The free energy barrier computed by WTMetaD+IB is 28.4 ± 0.4 kcal, which is consistent with standard WTMetaD (Fig. ).