Using a UV-VIS Spectrophotometer, an absorbance spectrum was obtained(Figure ), to evaluate the absorbance tendency. The UV-Vis absorption spectrum shows that the ZnO excitation absorption band at 380 nm has extended to the violet/indigo area, compared to the bulk absorption edge (about 400 nm) for ZnO NPs. This can be attributed to the nano size effect of biosynthesised ZnO NPs . Thus the absorbance values confirm, to an extent, the presence of ZnO NP . The presence peaks for carbon, primarily seen in the range of 180-260 nm in all the carbon materials/impurities, was relatively low in absorbance as compared to the ZnO NPs.