The motion of the bases within this process was also given rotation degrees of freedom around a fixed atom of the base bonded to the sugar-phosphate backbone. It was determined that the hydrogen bonds between the bases extended quasi-linearly during DNA strand separation and that the bases rotate to preferentially limit the extension of the upper and lower, but not central, hydrogen bonds between the G-C base pair. Additionally, the MD investigation provided estimations for the timescale of DNA stand separation. The authors concluded that the lifetime of the tautomeric state must outlive 1.7 ps -a speed two orders of magnitude smaller than quoted by Florian and Leszczynski. In this letter, we model the tautomerisation of A-T during DNA strand separation.