In this study, new naturally-derived quaternary ammonium salts (QASs) with quinine-based cation and two amino acid anions: L-asparaginate and L-alaninate were obtained for the first time using a sustainable, environmentally friendly synthesis method. and the acquired FT-IR and NMR spectra allowed confirmation of their chemical structures. The new compounds exhibited many times higher solubility in water compared to known 1-alkylquininium bromides. The antimicrobial activity of the analyzed quinine-based QASs increased with the elongation of the alkyl chain in the cation, and the chemical structure of an anion had no influence on this effect. The 1-dodecylquinium salts inhibited the growth of most of the microbes tested at concentrations below 16 mg dm -3 , thus showing activity at the level of