As mentioned in Section 3.2, a small peak, named VP3 prime, was detected between VP3 and VP1 during separation. Processing parameters in BPF were modified to try and identify this feature, as it is not detected within the parameters currently utilized (Table ). Here the samples separated at 25°C with an MS resolving power of 45K and the samples separated at 45°C with an MS resolving power of 15K were reprocessed as these samples provided the best identifications at their respective MS resolutions. The reprocessed data suggests that this feature contains a mixture of multiple VP3 proteoforms, predominately deamidated forms. At a resolving power of 15K, the prominent feature detected was a deamidated VP3. At a resolving power of 45K the highest scoring features detected were a deamidated VP3-Ox, a VP3-2Ox, and a deamidated VP3-Oxidation to kynurenine. These finding suggest that on-column oxidation or deamidation might be occurring, but alone are not sufficient to conclude this. Further investigation is required to determine the exact nature of this peak.