To our knowledge, this represents the first high-throughput approach for conducting a Hill plot analysis of anion transport. Our assay provides a foundation for further advancements in highthroughput methodologies. Given the current intense research interest in ion transporter design, we hope that our methods will accelerate the pace of discovery and translation in this area. The enhanced capacity for data generation will allow for the exploration of larger anionophore libraries. This broader exploration of chemical space will contribute to a deeper understanding of structureactivity relationships and aid in the identification of new design principles. By making these highthroughput methods accessible to the research community, we aim to alleviate the experimental bottleneck in ionophore screening, ultimately advancing research and facilitating the discovery of novel biologically active transporters.