Transgenic male tgAPPSwe mice, 18 and 23 months of age (n=3 per age group), carrying the Swedish (K670N, M671L) mutations of human APP were studied. The animals were reared ad libitum and housed in groups at an animal facility at Uppsala University, Sweden, under a 12/12 light cycle. The animals were anesthetized with isoflurane and sacrificed by decapitation. The brains were dissected quickly within >3 min postmortem delay and frozen on dry ice. All animal procedures were approved by an ethical committee and performed in compliance with national and local animal care and use guidelines (DNr #C17⁄ 14 at Uppsala University). For this study no randomization, blinding, and sample size calculations were performed. All animal experiment and reporting was performed according to the ARRIVE guidelines. This exporatory study was not preregistred and not randomized. No inclusion or exclusion criteria were applied. No sample size calculation was performed, though poshoc power calculations were included for validation (see Data Ananlysis).