It seems that reaction even with Ac2O, in the absence or minimisation of basic catalyst, allows for fine control over the enzymatic digestibility. For the purposes of this study pyridine was used as base, as it is a classical reagent used in catalytic or more stoichiometric reactions. However, it is not without its hazards. Fortunately, there are a wide range of organic and inorganic bases available that have more favourable hazard profiles.