We measured the amount of DNA origami accumulation in the DNA condensate by their fluorescence intensity (Figure ). The accumulation of DNA origami increased with increasing the binding valence, and the both-sided binding design showed more accumulation than the one-sided one. Next, we quantified the efficiency of DNA origami accumulation on the DNA condensate surface by calculating the ratio, I surface /I internal , where I surface and I internal denote the surface and the internal intensities of the DNA condensates, respectively (Figure ). I surface /I internal in the one-sided binding design was much higher than that in the both-sided binding design, indicating that the one-sided binding design had a more efficient coating feature, while the condensate-binding tags on both faces promoted the penetration of the DNA origami into the DNA condensate. In the case of the one-sided binding design, there was a slight trend of the increase in I surface /I internal with increasing the binding valence. These results suggest that the one-sided binding design of 3D DNA origami with more valences will be a better design for coating DNA condensates.