G*-T, whereby the reactants start as a wobble mismatch and, via proton transfer, result in a Watson-Crick-like conformation. We determine that the reaction wobble(G-T) to G*-T proceeds through a G-T* intermediate state in a step-wise mechanism. The minimum energy pathway can therefore be described by two steps; in the first step, the wobble(G-T) passes through the transition state of wobble(G-T) ⇀ ↽ G-T*. In the second step, through an intermediate local minimum and, the G-T* intermediate converts to G*-T. In comparison, the G-T* reaction (reaction 1) contains one transition state with no intermediate minimum.