The target number of molecules to keep is calculated from this user defined proportionality value. • If the calculated number of points of a cluster to keep is less than 15, then random sampling is used to select the members of the cluster that are kept as well as one which is taken as the nearest neighbor of a fitted Gaussian's mean (cluster centroid). This is to aid in downsampling accuracy, as Poisson disk sampling may yield greater or fewer molecules than the target with proportionately significant disparity, due to spatial constraints, when the target value is this low. • If the calculated number of points of a cluster to keep rounds to 0, then the point closest to the cluster centroid is kept.