The IC50 values for the compounds ranged from 0.7 to 8.7 μM, demonstrating that these drugs are promising hit compounds for the development of antimitotic chemotherapeutics. of the microtubule growth rate and catastrophe frequency in the presence of the tubulin ligands at the concentration specified in panel b. Each data point is a mean value from an independent experiment. Statistical significance was assessed using an unpaired t-test with Welch correction. ns -not significant; *, *** -p-value (two-tailed) < 0.05, < 0.001, respectively. d) Quantification of the fraction of cells in the G2/M phase in the population as a function of the drug concentration based on the distribution of the DNA content. Data points represent the means of N = 2-3 independent experiments. The upper gray zone indicates the mean ± standard deviation for cells treated with 0.3 or 1 μM nocodazole, used as a positive control. The lower grey zone indicates the mean ± standard deviation for cells treated with DMSO, used as a negative control.