(1) 2.5. Fourier Transform Infrared Spectroscopy (FTIR) FTIR was used to examine the change in the secondary structure of neat lyophilised BSA powder at 278 K. F2 was analysed at 278 K and after being heated to 370 K. F4 was analysed at 278 K and after being heated to 410 K. F6 was analysed at 278 K and after being heated to 330 K. F7 was analysed at 278 K and after being heated to 370 K.