Liposomes were prepared by the standard lipid film hydration method. the mixture of phospholipids and cholesterol (8.1 mg DPPC, 1.1 mg DPPG, 0.9 mg cholesterol) was dissolved in 10 ml of methanol:chloroform solution (1:1 by volume). Subsequently, the solvent mixture was evaporated on a vacuum rotary evaporator (60 °C, gradually reducing the pressure from atmospheric to approximately 80 mbar). This process produced a dried lipid film which was subsequently dried in a desiccator for at least 3 hours (30 mbar).