The TGI level of responses for (aR)-6 and (aS)-iso-6 were subsequently compared to known tubulin interactors using a pattern recognition algorithm (COMPARE analysis, Figure ). Many of these known compounds had been tested at multiple different high concentrations, and a single set of high concentration tests was chosen for each compound where it displayed the most robust selectivity pattern. Notably, (aR)-6 showed a meaningful correlation (Pearson correlation > 0.5) to (-)-colchicine, while (aS)-iso-6 fell short. However, a few tubulin-targeting molecules had a significant correlation with both molecules -the colchicine site binder centaureidin, the vinca site binder dolastatin 10, and taxol. However, the closest correlation was in fact between (aR)-6 and (aS)-iso-6, supporting a shared mechanism of action. While several well-established tubulin inhibitors did not have meaningful correlations with either molecule, enough positive correlations supported testing the compounds in biochemical assays. and polymerization inhibition at select concentrations, as well as 50% growth inhibition concentration (GI50) of select cell lines, along with standard error. -denotes that an experiment was not performed, and > denotes that the no binding or growth inhibition was observed within the detection limit of the assay.