Additional recombinant antigen for assay development and validation was produced in-house by the AuCoin laboratory. The RVFV MP12 strain NP sequence was retrieved from GenBank (accession number AF134530.1). This sequence, including an N-terminal GS linker and polyhistidine tag, was cloned into the pcDNA3. The LFIA strips were assembled using a 8980 conjugate pad (Ahlstrom, Helsinki, Finland), a UniSart CN95 nitrocellulose membrane (Sartorius, Germany), and a CFSP203000 absorbent wicking pad (EMD Millipore, Billerica,MA), all mounted onto an adhesive backing card (DCN Dx, Carlsbad, CA). The conjugate pad was pre-treated with a blocking buffer consisting of 10 mM borate buffer and 0.25% Triton X-100 (Sigma Aldrich, St. Louis, MS). The nitrocellulose membrane was pre-treated with a blocking buffer containing 10 mM sodium phosphate, 0.1% bovine serum albumin (BSA) ( Sigma Aldrich), 0.2% PVP-40 (Sigma Aldrich), and sucrose (Sigma Aldrich). The BioDotXYZ3060 was implemented in the striping of nitrocellulose membranes. The capture antibody of the RVFV LFIA was dispensed on the test line at a concentration of 1.0 mg/mL, while goat anti-mouse IgG polyclonal antibodies (Southern Biotech, USA) were applied to the control line at 1 mg/mL. Finally, the strips were cut to a width of 4 mm.