In recent years, as a unique category of fluorophores, luminogen with aggregation-induced emission (AIE) characteristic has been extensively explored in biomedical fields. In particular, the photophysical properties of excited AIE luminogens (AIEgens) (i.e. fluorescence, photothermal conversion ability, and ROS generation capability) can be readily tuned by restraining or assisting the intramolecular movements of AIEgens. For instance, by constructing the unique confined microenvironments of AIEgens, the active molecular motion-related nonradiative thermal relaxation can be markedly restricted in aggregate state, consequently sparing more energy to synchronous enhancement of fluorescence emission and ROS generation in a single system. The performance of AIEgens in respect of fluorescence brightness, photothermal ability, and ROS generation can also be coexisted and rationally manipulated in a single system on the basis of the propeller-like twisted configuration and free rotational/vibrational conformations of AIEgens. In addition to integrating fluorescence, photothermal and photoacoustic multimodules in one single theranostic nanoagent, rational design of structure can endow the AIEgens with intrinsic cytotoxicity, which can be regarded as an effective drug. Therefore, developing AIE-based one-for-all nanoplatforms to coalesce various functional modalities for enhancing efficiency of precise theranostics is tremendously promising.