After optimization, we aimed to test the automated delipidation workflow on nanodisc samples. Nanodiscs utilize a membrane scaffold protein (MSP) -a derivative of apolipoprotein A-1 -capable of encasing an inner lipid core, providing a phospholipid bilayer for membrane protein solubilization . Thus, the MSP protein exists in lipid-free as well as nanodisc form, which makes this type of lipid vesicle an ideal membrane protein system to test the performance of novel HDX-MS workflows.