For cell-based assays, dose-response curves and corresponding the half-maximal inhibitory/effective concentrations (IC50/EC50) were determined. For the biochemical assays, compound-targets showing an experimental readout greater than 25% (inhibition or stimulation) at 30 µM were selected for follow-up, and dose-response curve characterization and determination IC50/EC50 (two or three replicates, multiple concentrations, maximum 100 µM concentration). IC50 values were determined by non-linear least squares regression analysis, using RStudio v. 1.3.1073 (PBC, Boston, MA, ), MathIQTM (ID Business Solutions Ltd., UK) and GraphPad Prisma v. 9.1.0 (GraphPad Software, San Diego, CA).