Extracellular vesicles (EVs) are membranous particles released by diverse cell types. EVs participate in intercellular transport and communication. Their cargo may include proteins, nucleic acids, lipids and cell metabolites, which can be valuable biomarkers of human diseases or metabolic changes. Mass spectrometry (MS) analysis of the EVs' cargo composition requires isolated and disintegrated/digested vesicles. The EVs isolation from biological samples (e.g., blood plasma, urine) is laborious and timeconsuming and includes ultracentrifugation, filtering, size-exclusion chromatography, or affinity-based purification on functionalized beads. Recently the characterization of charged liposomes based on their gas-phase mobility was introduced as nES GEMMA (nano-electrospray gas-phase electrophoretic mobility molecular analyzer) and was applied as a separation stage for Raman and mid-infrared spectroscopy. Established separation techniques usually require larger amounts of material, which can be limited in samples from children or in the case of cerebrospinal fluid. The size of isolated vesicles is then characterized by dynamic light scattering, nanoparticle tracking analysis, flow cytometry, or transmission electron microscopy, which further reduces the yield of EV separation. The size of analyzed EVs is a critical parameter. There is a large variety of EVs produced by cells and those within a smaller range (50-300 nm in diameter) are considered as more informative about the cell state or the local environment than large EVs. Large unilamellar lipid vesicles (LUVs) are a widely used model system for studying EVs, particularly because they can mimic some aspects of the structure and properties of EVs. LUVs can be used to gain insights into fundamental properties like membrane interactions, fusion, and vesicle stability, but they cannot fully model the intricate biological functions of EVs that arise from their complex composition and biogenesis. 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine used for synthetic vesicle preparation is a common lipid mimicking cellular membranes in model systems. Recently, joint efforts of Jarrold and Clemmer groups at the University of Indiana have yielded a new approach to studying intact EVs. In particular, the intact EVs were characterized using charge detection mass spectrometry (CDMS) with nano-electrospray. The precisely measured charge and m/z of the EVs transported into the vacuum and characterized by the charge detector provided exact mass and charge distributions, the last one reflecting structural and surface information. CDMS enabled the distinguishing of keratinocytic exosomes in diabetic and non-diabetic mice based on the charge distribution subpopulations retrieved by a Gaussian mixture model. CDMS instrument used in these studies consisted of multipole non-resolving ion guides, acting simultaneously as a high-pass filter, to transfer charged particles generated in nano-electrospray from atmospheric pressure toward a multi-pass charge detector in vacuum. In other investigations charged particles with m/z>10 5 were also analyzed using electrodynamic traps, including different designs of Paul and linear quadrupole traps . It is worth noting that MS using linear quadrupoles is generally limited to m/z < 10 5 with relatively low quadrupole resolution. For even higher m/z the quadrupole driver electronics must feature extraordinary specifications, and a few special approaches to the quadrupole operation were introduced, i.e., using the frequency scan or digital modes . In this work, we showcase the resolving regime of frequency-scanned quadrupole in m/z 10 5 -10 6 for the separation of synthetic lipid particles -LUVs using SELINA (Selected Ice Nanoparticle Accelerator) instrument. SELINA was designed to produce mass selected beams of highly charged water ice nanoparticles with 50-1000 nm diameter mimicking space dust found in the vicinity of several icy moons in our Solar system. Impact mass spectrometry of such particles accelerated electrostatically to hypervelocities is supposed to be the best terrestrial analog experiment for past and future space missions with impact ionization MS instrumentation onboard. Moreover, it is the first step towards the development of novel, high resolution, space probes based on impact ionization MS. The SELINA design is also suitable for the production and measurement of sub-micrometer solid particles from liquid samples nebulized by electrospray. A special care must be taken to fully desolvate the particles. In fact, this procedure was successfully carried out for 300 and 600 nm polystyrene beads during the charge detector calibration experiments. Here we present an operational mode of SELINA, which enabled us to isolate and weigh LUVs from aqueous samples.