Similar bulky residues have been identified as membrane anchors in other peripheral membrane proteins. The small variation in bound residues and cumulative contacts over an additional microsecond of simulation suggests that the protein-substrate complex may occupy a stable position upon the membrane in which the N-terminus and the 235-245 helix make two points of contact with the membrane surface during prenyl chain extension. Protein-membrane dissociation events were not observed at any point during these simulations(Figure ).