As we had orthogonal UV and blue photocages that could be attached to amino-C6-dT modifications, we next aimed to generate two ASOs that would bind different mRNA and selectively degrade their target with each wavelength (Fig. ). To allow the fluorescent measurement of a different target in CFPS, we aimed to identify a new ASO sequence that could target the mRNA encoding for the red fluorescent protein mCherry (mC), without binding mV-mRNA. Being derived from fluorescent proteins of different organisms (dsRed vs. avGFP), mC has an orthogonal excitation/emission spectrum to mV and a large difference in the DNA sequence. After screening several ASOs to identify a good target point in mC-mRNA, with sequences containing multiple Ts across the length of the ASO (Supplementary Fig. ), a second screening process was carried out to find a sequence with orthogonality to mVenus (Supplementary Fig. ). The same test was also performed with the mVenus ASOs, in which we identified that V2 was orthogonal to mC, whereas V3 showed some crosstalk (Supplementary Fig. ).