UmetaFlow was validated and parameter-optimized (Table ) with in-house LC-MS-MS/MS data obtained from an UHPLC coupled to a Thermo Orbitrap IDX mass spectrometer from extracts of actinomycete strains that are producing known secondary metabolites, as well as commercial standards. This validation was performed at a pair of Intel(R) Xeon(R) CPU E5-2695 v3 @ 2.30GHz, with 14 cores per socket and 2 threads per core, with 512GB of RAM.