Decarboxylation of coumaric acid 1a, caffeic acid 1b, ferulic acid 1c, and sinapic acid 1d catalyzed by cell-free extracts of PAD was performed in 500 mg-scale. For the successful decarboxylation of sinapic acid, the variant BsPAD I85A was employed. This mutant was created analogous to a mutant of Bacillus pumilus PAD and has increased activity towards p-hydroxycinnamic acids bearing two substituents in meta-position. With all four phenolic acids investigated, the reactions proceeded quickly to completion as shown in Scheme 2, and the reaction mixture was immediately used for experiments on the O-methylation.