Da and the higher CNLs are not possible (i.e., precursor ion mass < CNLs) for more and more compounds. Additionally, a distribution of the fragment m/z counts was also generated for comparison (Figure ). Here it can be seen that the fragment m/z counts are less clearly defined compared to the CNL distribution (Figure ). Moreover, when comparing the number of unique CNL to fragments, using the mass window of 0.001 Da, a ≈4 fold reduction was observed (i.e., 216,974 fragments to 61,803 CNLs). Finally, the CNL counts were converted to probabilities and still the same trend was observed (Figure ). However, the probabilities in the TN distribution are much smaller than the TP probability distribution due to the larger number of TN cases than TPs. Therefore, a score CN L is calculated that evaluates the relationship between P (T P ) and P (T N ). To provide an idea of what these probabilities mean in terms of chemical information, an overview of a few CNL masses with high TP probability can be found in table to the neutral losses of the precursor ion, meaning that a fragment mass might not have the same m/z value for multiple precursor ions while their CNL mass with that fragment is actually the same. Moreover, the CNL occurrence probabilities could potentially be used to assist fragmentation pattern prediction tools, by, for example, ranking the fragments based on their CNL occurrence probability.