We have applied the LCA methodology to evaluate the environmental performances of an innovative route to produce a new whole fish oil (AnchoisOl) and a new organic fertilizer (AnchoisFert) starting from the anchovy fillet leftovers citrus-derived limonene both as a solvent and as stabilizing (antimicrobial) agent. The analysis of the process was referred to laboratory (LS) and industrial scale (UP). The quantitative assessment enables to evaluate the environmental impacts associated to each single flow and production step.