Second, we screened all fractions that exhibited biofilm inhibition for biofilm disruption properties. Biofilms for each pathogen were pre-formed and then treated with each fraction. The biofilms were incubated with the fraction overnight, washed, and the remaining biofilm quantified. Only six fractions disrupted > 50% of S. aureus biofilms (15.7% of the biofilm inhibitors), while no fraction significantly disrupted P. aeruginosa biofilms (Fig. ). The fractions that disrupted S. aureus biofilms were produced by Bacilli (three), Actinomycetia (two) and Flavobacteriia (one).