Just after time = 60 seconds, the trap contents were CO2-laser heated again for a few seconds, but at 2% duty factor (~0.2 W). While the CO2 laser was on, the APD signal increased to ~350,000 counts/sec, and after the CO2 laser was turned off, the 532-nm-excited emission intensity was ~2.5 times larger than it had been before 2% duty factor heating. In addition, after this second heating pulse, two Fz resonances were observed (green and blue dots), indicating that at least two NPs were present with Fz resonances in the 0.1 to 10 kHz scan range. It is possible that the appearance of two NPs resulted from fission of the original NP during heating. Note, however, that when NPs are first heated to new, higher temperatures, they often undergo thermionic electron emission, possibly shifting Fz for the second NP into the resonance scan range. The Fz associated with the original NP also increased, to 2.5 kHz, which could have resulted from mass loss from fission or from thermionic emission.