A preparative scale reaction using CLECs of HheG M45-D114C was performed in 10 mL 50 mM Tris•SO4 buffer, pH 7.0 containing 50 mM cyclohexene oxide (1), 100 mM azide and 2 mg CLECs. The reaction was stirred for 2 h at room temperature and 800 rpm. Afterwards, the reaction mixture was extracted once with 10 mL TBME, the organic fraction was dried over anhydrous MgSO4 and the solvent was removed by evaporation yielding the product as yellowish oil . The product was further analyzed by achiral and chiral GC (Table ).