For data collection at 100K, grids prepared with crystals were loaded dry into a cryo-transfer holder at room temperature, inserted into the microscope, and then cooled to 100 K prior to data acquisition. For the proteinase K crystals, which would collapse if dried out prior to loading into the TEM, grids were only studied under frozen-hydrated conditions at 100K. Crystals of the AVAAGA peptide were additionally studied under frozen-hydrated conditions to explore the impact of vitrification on beam-induced reorientation.