Understanding this, we developed a platform, using the CFR Part 11 compliant software Chromeleon, capable of both, rapid serotype identification and serotype specific PTM monitoring for product quality. Analysing serotypes AAV2, AAV5, AAV8, and AAV9, we demonstrated how our rapid serotype identification method can be universally applied to accurately determine the serotype in each VP separation analysis, even for runs with modified MS and LC parameters. Using AAV2 as a proof of concept, we then developed a method that can be used to characterize PTMs in different AAV serotypes. Such a method can then be used to monitor PTMs across various production batches to help ensure product quality. This method can easily be adopted to other serotypes for broad use analyzing AAV products. Within Chromeleon™ both methods can be applied to the same chromatographic runs, because of the separation capabilities and improved spectral quality that DFA as an ion pairing agent provides, allowing both rapid identity testing and PTM characterization within a single analysis. Additionally, being designed within QC-compliant software, this platform enables