The analysis of the MD trajectories showed similarities between dynamic behaviors but also differences that can be potentially captured and exploited using 3DDPDs. Such differences can be better observed aligning the Root Mean Square Fluctuation (RMSF) values to a GPCR class A MSA (Figure and Figure in Additional file 2, that contains all supplementary figures). Across GPCRs there is a shared pattern of reduced mobility in the TM domains compared to extracellular (ECL) and intracellular (ICL) loops or N-and C-terminus.