We implemented linearly decreasing crossflow profiles to overcome the limitations of recovery and resolution (Figure , center). This crossflow profile was suggested by Gigault et al. for fast separation of two species with acceptable resolutions of 𝑅 𝑆 > 1.0. Initial crossflow rates are high enough to provide sufficient separation, as documented by resolutions of 1.0 (for 0.4 mL/min) to 1.3 (0.6 mL/min). Again, peak broadening indicates an aggregation tendency for higher crossflow rates. The average crossflow rates are lower; consequently, the adsorption of particles on the membrane decreases, and the recovery increases up to 73-79%. However, significant losses of > 20% still occur due to crossflow-independent adsorption on tubes and walls as described above. For 0.5 mL/min and a linearly decreasing crossflow profile, a resolution of 1.2 and a recovery of 79% is obtained, and sufficient separation success is achieved.