Neutron scattering studies have shown that cholesterol is preferentially located in the bilayer center, parallel to the membrane plane, in poly-unsaturated lipid bilayers, namely composed of DAPC 103 , as well as in cases where the extended length of a cholesterol molecule (15 Å) exceeds half the width of the acyl chain region 104 . This matches well our observation for the preferential location of our Martini 3 cholesterol model in the center of DAPC bilayers. It must be noted that experimental structural studies have only observed this non-standard location of cholesterol in these previously mentioned cases. Studies in DOPC membranes have shown that cholesterol is predominantly inserted in its canonical upright orientation (which matches the behavior we observe in our Martini 3 model); however, it is unclear whether there is room in these experimental findings for a minor population in the center of the bilayer.