The results shown in Figure reveal that the key impact contributing activities within process step I are the consumption of ozone reagent and the use of methanol solvent. Therefore, the reduction of ozone and methanol consumption in step I represent important tasks for impactful process optimisation. In this study, ozone reagent was fed at 87% excess to the ozonolysis reactor, as initial experiments showed that product yield was lower for nearstoichiometric ozone feed and plateaued for larger ozone supply at the tested operating conditions. By investigating yet unexplored ranges between near-stoichiometric and 87% excess ozone feed, and by fine-tuning residence time and other operating parameters, further research should reduce the overall ozone consumption without loss in product yield.