Along with experimental studies, the mechanistic aspects of folding-unfolding events in various tetraloop hairpin RNA motifs received significant attention by several computational methods in recent years. The molecular dynamics simulation with various enhanced sampling techniques show promising results in understanding the key intermediates and transition events involved in these processes. It is evident from the literature that the many physical properties of biomolecules including various RNA motifs show marginal difference in the presence of chiral small molecules. Even though the dynamics of various RNA tetraloop motifs are studied extensively using molecular dynamics simulations, the impact of chirality-dependent folding -unfolding studies using computational studies are not well explored. The molecular driving force and mechanistic aspects causing the specific effect of enantiomers on foldingunfolding events remain as a mystery. Molecular-level understanding of these processes is necessary to comprehend and solve many such scientific ambiguities related to enantiospecific dynamics of RNA motifs. These challenging aspects of chirality-dependent folding-unfolding problems and experimental evidence motivated us to provide molecular insights into the foldingunfolding of GAAA tetraloop in the presence D-arginine and L-arginine using multidimensional free energy landscape aided by enhanced sampling techniques. We hope that our discussions on chiral dependencies on RNA dynamics will be beneficial for further small molecule dependent enantiospecific studies of various biomolecules and comprehend chiral amino acid-based theories on origin of life.