Preparation of CyP-OBu-loaded vesicles. CHCl 3 was evaporated to form a thin lipid film that was then hydrated with 10 mL distilled water heated at 60°C and sonicated for 30 min (30W). All subphases were prepared with pure Milli-Q water (Millipore, resistivity 18 MΩ cm, surface tension of 72.8 mN•m -1 at 20 °C). Finally, the solution was filtered through polycarbonate membranes with a pore size of 0.450 µm in order to remove the titanium particles released from the sonication tip, and stocked at 4°C.