The SER results showed that molecules with different orientations interact differently with the electric field environment excited by different polarizations of light, indicating that the OCB system provides a qualitative system for differentiating molecular orientations. To expand the application of the OCB system, the SER response of 4ABA functionalized MNPs depositing on the OCB was also tested, revealing polarization-dependent distinctions to the AuAg substrate upon deposition of the MNPs. However, further investigation is needed to fully understand the complexity of the OCB-nanoparticle interface environment.