For the lipid analysis in serum, the LC-HRMS raw data files were processed in MS-DIAL (parameters given in Table ) using the in silico LipidBlast library. To complement the MS-DIAL lipid annotations, which are similarity-based, LipidMatch , a rule based software, was employed (see Figure ). The annotations from MS-DIAL and LipidMatch were combined; LipidMatch annotations were selected in case of duplicates.