For instance, LC separation performance and drift can be monitored by known retention time peptide spikes like the PRTC or iRT, albeit using IDs of endogenous peptides themselves can also provide metrics by modeling predicted retention times . When proteins or proteomes are used true digestion efficiency can be calculated along with ID-based metrics like tryptic miscleavage rates to accurately qualify digestion performance. Additionally, quantitative standards can be used to determine detection limits, either of a targeted assay (Pierce System Suitability Standard 7 x 5 mixture) or of isobaric labels (TMT11 yeast digest). Moreover, different data acquisition techniques may give unique performance measures, such as isotopic and isobaric labeling, that can capture labeling efficiency and channel missingness . These metrics are useful in that when there is a problem between sample processing and data analysis, troubleshooting can be directed by the results.