Polarization and Impedance curves have obtained from specific empirical operation condition. MEA structure defines as composition of anode, membrane and cathode. Temperature of anode, cathode and cell, pressure and flow rate of H2/O2 (ml.min -1 ) have been considered as operation condition during the evaluation. In Polarization curves, cell voltage (V) per current density (mA.cm -2 ) and cell power density (mW.cm -2 ) per current density (mA.cm -2 ) has been obtained at various relative humidity, gas pressure and membrane compression. Impedance analysis were done at the end of the each activation set and procedure at different cell voltages, relative humidity and H2/O2 pressure. Also, in each activation procedure, analysis has been accomplished by repeat of activation sets .