1. Click on the "Start new session" button. This action sets all the string variables to blanks and integer variables to zeros and clears the textboxes. 2. Enter a project title of choice in the top most text box of the UI (optional). 3. Enter the id of the query sequence, the accession number for example, in the short textbox located at the far left of the third row in the UI. 4. Enter the query sequence in the textbox located next to the sequence id box in the third row of the UI. Leave all other text boxes empty and then click "Enter" (While querying sequences, the sequences are copied and pasted in this textbox one at a time).The output appears in the display box which is a large textbox in the fourth row of the UI. The output consists of a Group label with a group number and the sequence itself followed by a serial number within the group as shown in Figure . Newly entered sequences will appear in new groups if they are not identical to any of the previously queried sequences.