Cryo-micrographs were acquired on a Talos L120C G2 microscope (FEI/Thermo Fisher) operated at 120 kV, using a 4k × 4k BM-Ceta camera, an objective aperture of 100 µm and nominal magnification of 4200-92000. Grids were inserted into the microscope using a side-entry Dual Grid Cryo Transfer Holder Model 205 from Simple Origin, Pittsburgh, USA. Three independent rounds of liposome preparation were screened with at least 4 grids per sample at each round.