To ensure the modeled metabolic pathways (described above) are relevant to more than just individual model organisms, an enzyme conservation analysis was performed across 69 key enzymes spanning 16 metabolic pathways (Table ). The presence or absence of each enzyme was evaluated across data from 11 microalgae metabolic reconstruction data sets from seven different microalgae species: Chlamydomonas reinhardtii, Nannochloropsis oceanica, Chlorella protothecoides, Chlorella vulgaris, Chlorella variabillis, Ostreococcus lucimarinus, and Ostreococcus tauri. If the enzyme was present, it was marked as conserved in that data set for the specific microalgae species. A literature review for experimental evidence was also completed for enzymes that were not included in the metabolic reconstruction data sets but were believed to be present in microalgae metabolism (additional details in Section S1 of the SI).