After running this command, Infliximab_pqr.pqr file was generated, and the titration states of each histidine could be determined. Figure shows that residue 32 on the heavy chain was predicted to be HSD. We can modify the Infliximab.tcl to add this mutation to change the titration state. After modifying all the histidine titration states, we can rerun Infliximab.tcl script to generate the final PDB and PSF files.