Minimum distance analysis for transmembrane proteins. For a given lipid species of interest, we tracked as a function of time if each lipid molecule satisfies a distance cutoff of 5 Å from any protein residues and from selected protein residues that define binding sites: For Kir2.2, in each monomer the minimum distance was computed for residues R78, W79 and K188; for the ADP/ATP carrier, we used residues 272-274, residues 72-74 and residue 176 for CDL sites 801, 800 and 802, respectively. The analysis was performed using the Distance analysis module from MDAnalysis 207 .