BIOMX-DB was built using Next.js, a common web app framework known for its performance and SEO optimization. It employs a single-page application (SPA) architecture with three tiers for clear separation of concerns: frontend, backend, and database. The frontend utilizes HTML, CSS, JavaScript, and libraries like React and Material-UI to enhance the user interface. Meanwhile the backend leverages Node.js as its runtime environment and runs on an Nginx web server, JavaScript was used for internal APIs while python was used for molecules 'calculations. MongoDB, a NoSQL database, stores and manages the application's data. Table summarizes additional technical details for the creation of BIOMX-DB.