Comparing the two pressure profiles for the cis and trans membranes together show only modest changes between the two membranes, with somewhat greater compaction possible due to the negative pressure difference near the membrane interface. The repulsive term near the membrane center is effectively the same, in agreement with prior simulations tying that feature closely to the degree of unsaturation within the lipid bilayer. Since we only change the isomerization, but not the degree of unsaturation, the profile midpoint is mostly unchanged. Cis lipids tend to have a higher repulsion term and have higher peaks in the lateral pressure profile where the unsaturation sites are farther from the membrane midplane (Fig. ). Trans lipid membranes have properties more similar to saturated lipid membranes, with more negative pressures when compared to the cis membranes. The decrease in the lateral pressure profile is also linked with the fluidity, as shown in a previous study in which adding cholestrol to lipid membranes increases the pressure profile for the tail region. Stiffness or reduced diffusion that we observed for trans membrane can be correlated to lateral pressure exerted by the tail region.