First, we measured the discriminating power between kinases by comparing KiSSim fingerprint distances between DFG-in structures of the same kinase and of different kinases, i.e. intra-kinase and inter-kinase distances, respectively. With a median of 0.02 compared to 0.11, the (about 200000) intra-kinase distances are significantly lower than the (about 8.2 million) inter-kinase distances as shown in Figure , indicating that the fingerprint can discriminate between kinases. Note that the distances between structure pairs describing the same kinase pair can vary a lot (Figure ); for the all-against-all comparison, we consider only the most similar structure pair per kinase pair.