To provide additional evidence of the efficiency of lipid removal from blood products with the developed protocol, 1 H 1D NMR spectroscopy was used to compare spectra obtained for the dSPEtreated and untreated plasma (Figure ). It can be clearly observed from the 1D spectra that the broad lipid signals, that can be assigned in the untreated plasma samples to the terminal CH3 and various types of CH2 and CH groups from long-chain saturated and unsaturated fatty acyls of lipoproteins , are to a large extent removed from the dSPE-treated plasma sample. The singlet at 2.04 ppm, corresponding to acetyl groups of glycoproteins, is not present on the spectrum of dSPEtreated plasma due to the precipitation of proteins occurring simultaneously with the lipid removal.