Early in the cycle, i.e., after 6 or 15 hours, the vesicles comprised almost equal parts of the C6P, C8, and C8C6P. Later in the cycle, we found that the fraction of C6P had increased at the cost of both C8 and C8C6P. Noteworthy is that the reaction cycle with C8 and C8P also produced vesicles, as evidenced by confocal and electron microscopy (See Supplementary Figure and).