4. Stacks (Fig. ) were subtracted by a background corresponding to the medianaverage of around 10 frames corresponding to the image area prior to platelet growth (Fig. ), which generates the background corrected result as shown in Fig. . 7. After setting the measurement scale, particle parameters, such as area, long/short axis length, and aspect ratio were then collected using the built-in 'Analyze Particle' function in Fiji. iv