For stability validation, large sterol containing bilayers 30 × 30 nm 2 were simulated at 20 fs for over 5 microseconds. For sterol behavior validation, 10×10 nm 2 DPPC lipid bilayers containing increasing concentrations of sterols were simulated at 323 K. Several membrane biophysical properties were then probed, such as area per lipid, membrane thickness and acyl chain order.