Multivalent lectin-glycan interactions (MLGIs) are widespread and play a pivotal role in pathogen infection, immune regulation and cell-cell communication. It is thus unsurprising that glycan displaying nanomaterials have been widely exploited for potential antiviral and immunotherapeutic applications, due to simple polyvalent glycan functionalization. For glyconanomaterials, the geometric parameters, such as size and shape of nano-scaffolds, inevitably affect their surface glycan display. As natural lectins are often multimeric, variations in glycan display may lead to changes in the number of binding sites that can be occupied at one time, which dictates the strength and mode of lectin-glycan binding and, subsequently, its biological function. A large number of nano-scaffolds with different geometries have been employed to target lectins. These include spherical scaffolds (e.g. inorganic nanoparticles, fullerenes, dendrimers, hyper-branched polymers, polymersomes, etc), tubular structures (e.g. carbon nanotubes, cylindrical micelles), planar sheets (e.g. graphene nanosheets), as well as structures that are less well-defined (e.g. linear polymers) or more complex in shape. Since these results are obtained with scaffolds of different glycan composition, softness, size, shape, and glycan density, it is difficult to directly compare results from one another to draw general conclusions. While a few studies have shown that the scaffold size and shape can affect their lectin binding and viral inhibition properties, the molecular mechanisms underlying such differences remain unclear. Therefore, new tools need to be developed in order to identify the rationales behind these differences. In this regard, probes that can reveal how scaffold shape affects MLGI binding mode and affinity are highly valuable, allowing us to establish a geometric design rule for glyconanoparticles for potent and specific targeting of particular MLGIs for therapeutic application.