Step 1: Target Identification. In this step, the low-fragmentation is-CID mass spectrum of the mixture is used to identify a set of target mass-to-charge ratios (m/z) with relative intensities greater than a user prescribed threshold (e.g., 5 % relative intensity). Each of these target peaks are considered representative of one or more potential pure components in the mixture. There have been no updates to this step of the algorithm.