Table : Free Energies (in kcal mol -1 ) for removing specific components from (G) 1 (A) 2 (SA) 2 at 298 K. "Frozen" and "Optimized" indicate whether the remaining cluster underwent reoptimization of geometry, while "Global Minimum" refers to allowing the remaining cluster to re-optimize to the global minimum. The computed free energies for removing various neutral and ionic components from the (G) 1 (A) 2 (SA) 2 cluster are summarized in Table , with details of the calculations provided in the SI. Note that the free energies listed in Table were computed for three different scenarios. Given the 30 ms activation time of the CID experiments, it is likely that the remaining cluster underwent geometry re-optimization. The "Optimized" free energies required for removing a single neutral A, G, or SA from the (G)