For Brewster Angle Microscopy experiments, a MicroBAM (KSV NIMA) was used in conjunction with a Langmuir Trough (KSV NIMA LB, S/N AAA100505). All solutions were prepared in MilliQ water with a resistivity > 18.1 MΩ. NaCl solutions were prepared using sodium chloride (Fisher) that was baked overnight at 200 °C to evaporate organic contaminants. Lipase solutions were prepared to a concentration of 16.5 mg lipase / 100 mL 0.4 M NaCl solution using lipase from Pseudomonas cepacia (BCL) (≥30 U/mg, Sigma Aldrich). LPS solutions were prepared to a concentration of 1 mg LPS / 100 mL 0.4 M NaCl using lipopolysaccharides from Escherichia coli (O111:B4, Sigma Aldrich). Hereafter, 0.4 M NaCl solutions containing either LPS, lipase, or no additional component (for control experiments) will be referred to as the subphase. Monolayers were prepared using a 1 : 2 : 4 : 3 ratio of LA : MA : PA : SA. Palmitic acid (>99%, Sigma Aldrich), stearic acid (>98.5%, Sigma Aldrich), myristic acid (>99.5%, Sigma Aldrich) and lauric acid (99%, ACROS) were individually prepared as 1 mg / mL chloroform (>99.9%, Fisher Scientific gold label) solutions. These four individual solutions were then mixed by volume according to the 1 : 2 : 4 : 3 ratio.