We expand the investigation of our device by demonstrating its application for two diverse applications, i.e., single-cell manipulation as well as microfluidic mixing. HeLa cells are a prominent model organism for biomedical as well as genetic research and their controlled manipulation exposes great potential to allow for further insights on a single cell level. The image sequence in Figure a) presents the slow and stable out-of-plane rotation of a single HeLa cell near an embedded microbubble with a wall thickness T = 5 µm, while the piezoelectric transducer is excited with a constant frequency of 69.2 kHz and voltage of 5 V P P .