Media loaded with 10 µM WinterGreen-WAY was irradiated at 561 nm for 800 seconds to check for aberrant release of WAY-161503. No response was observed, so we concluded that WinterGreen-WAY is not activated by extended irradiation of low intensity 561 nm light. To test for aberrant release of NDMC, media loaded with 10uM WinterRed-NDMC was irradiated at 561 nm for 800 seconds, then the media was spiked with 5 µM WAY-161503. The fluorescence intensity was compared to that of a positive control sample containing no WinterRed-NDMC in the media. No change in fluorescence intensity relative to the control was observed, so we concluded that WinterRed-NDMC is also unaffected by extended irradiation of low intensity 561 nm light.