Infected blood was taken from the tail vein of a donor mouse with a parasitemia level of 20% to 25% using heparinized capillary tubes and then transferred to a sterile plain beaker. Two milliliters of blood were diluted with ten milliliters of normal saline, yielding 0.2 milliliters of infected red blood cells. The mice were then given 0.2 mL of blood suspension intraperitoneally.