By enabling transformations in a fashion orthogonal to established chemical approaches, enzymes streamline the preparation of complex natural products and introduce new retrosynthetic disconnections and synthetic strategies. While dearomatization with Rieske dioxygenases 3 and lipase-catalyzed deracemization of early alcohol intermediates have found a solid foothold in contemporary natural product synthesis, several other classes of enzymes have recently made an appearance in routes toward natural products and their analogues. Owing to their versatility, enzymes have found applications in nearly all phases of (total) synthesis and continue to reshape the way organic chemists approach complex target compounds. As a primer to the state-of-theart concerning strategic uses of enzymes in natural product synthesis and the underlying concepts, this review highlights selected recent literature examples (covering 2020 to 2022) employing enzymes for the provision of chiral starting materials, endgame transformations, convergent synthesis and cascades facilitating complex reaction sequences (Figure ).