Having developed H2BK120-and H2BK43-selective LANA-DBM-BAHA catalysts 20 and 25, we synthesized H2BK108-selective LANA-DBM-BAHA catalyst 32 (Figure ). Acylation at H2BK43 or H2BK108 was previously reported; 24 however, how those PTMs modulate properties of chromatin is elusive. Therefore, we used three catalysts 20, 32, and 25 to compare functions of H2BK120ac, H2BK108ac, and H2BK43ac. Using LANA-DBM-BAHA catalysts with optimizing the reaction time, we produced recombinant mono-nucleosomes and 12-mer-nucleosomes bearing approximately 30% acetylation at H2BK120, H2BK108, or H2BK43 (Figures ), and then examined biochemical properties of these nucleosomes. It was reported that Mg 2+ -promoted nucleosome self-association was significantly inhibited by H2BK120ac. Using 12-mer-nucleosomes, the inhibition effect of H2BK43ac produced by 25 was even greater than that of H2BK120ac produced by 20 (Figure ). On the other hand, the effect of H2BK108ac produced by 32 was smaller than that of H2BK120ac (Figure ).