Circularly polarized luminescence. Owing to the stable helical cyclic skeleton within these chiral macrocycles, their electronic circular dichroism (CD) and CPL spectra were further explored. As shown in Figure , these four pairs of chiral macrocyclic AIEgens all exhibited strong and fully mirror-symmetric CD signals which indicated their enantiomeric structures. In pure THF, the chiral macrocycles RBT all showed strong negative first Cotton effects around 378 nm, while SBT exhibited positive ones. The identical CD bands of all RBT and SBT series indicated their similar chiral skeleton relative to RBT-OMe and SBT-OMe. And thus, the TAE units within RBT were all in P-configuration, whereas TAE moiety in SBT all adopted the M-helicity. In addition, the first cotton band around 380 nm also slightly res-shifted from R/SBT-H to R/SBT-2N, which also agreed well with the decreased E opt observed in UV-vis absorption curve. The CD bands of these CCMs also agreed well with their simulated CD spectra based on time-dependent (TD) DFT calculations (Figure ).