Proteins were separated by SDS-PAGE on NuPage 4-12% Bis-Tris gels and transferred to Amersham Protran 0.45 NC nitrocellulose membranes (GE Healthcare) using wet transfer. Membranes were blocked using 5% w/v milk in Tris-buffered saline (TBS) with 0.1% Tween-20. Blots were probed using anti-VHL (CST-68547), anti-CRBN (Novus, NBP1-91810) and anti-Tubulin hFAB-rhodamine (BioRad, 12004166) primary antibodies, followed by incubation with secondary anti-Rabbit IRDye 800CW (ab216773) or anti-rabbit HRPconjugated (CST-7074) antibodies. Blots were developed using a Bio-Rad ChemiDoc MP Imaging System or the Amersham ECL Prime Western blotting detection kit and Amersham Hyperfilm ECL film, as appropriate. Band quantification was performed using the ImageJ software. Band intensities were normalized to the tubulin loading control and reported as % of the average 0.1% DMSO vehicle intensity. Degradation data was plotted and analysed using Prism (Graphpad, version 6). DC 50 values (concentration to reach 50% maximal degradation) were estimated by fitting band intensity against log[concentration] with a onesite inhibition model. Apparent half-life values (time to reach 50% maximal degradation) were estimated by fitting band intensity against time using a single-phase exponential decay model.