Chignolin represents a common protein structural motif that undergoes folding and unfolding during brute force MD simulations. We tested the DeepWEST method by estimating rate constants between regions of interest, especially in the folded and unfolded regions. WE was run for a total simulation time of 120 µs averaged over three independent runs of 40 µs each. Similarly, the GaMD-WE approach ran six independent 500 ns of GaMD with varying boost potentials, and starting conformations were selected from the GaMD run that had the largest PES coverage. It was then followed by 39.50 µs of WE simulations, totaling a simulation time of 120 µs averaged over three independent runs of 40 µs each. Likewise, the Table : Rate constants (ns -1 ) between different metastable states for chignolin obtained after 40 µs of aggregate simulation time. In the case of brute force simulation, the first value indicates the average rate constant while the value within the brackets indicates the 95% confidence interval computed using Bayesian bootstrapping. For WE, GaMD-WE, and the DeepWEST methods, the error bars represent 95% confidence intervals obtained from the standard deviation of three independent runs. DeepWEST approach ran an unbiased MD simulation of 100 ns followed by 39.90 µs of WE simulation, totaling a simulation time of 120 µs averaged over three independent runs of 40 µs each. Figure demonstrates the ability of the deep-learned kinetic modeling approach to cluster the MSMs for chignolin. Initial conformations were selected from these metastable states for WE simulations. Figure and Figure represent the average free energy profiles of chignolin from the WE and the hybrid GaMD-WE approach, respectively, while Figure represents the free energy profile from the DeepWEST approach. Figure demonstrates the average free energy profiles of chignolin from three separate runs of WE, GaMD-WE, and DeepWEST simulations at different stages the simulation. The PES coverages for chignolin from the three approaches are comparable and demonstrates that the DeepWEST method can sample the thermodynamics of the system accurately.