Compositional analysis of mixture B and C shows the relative volumes of the compounds in the actual mixture compared to the analysis based on autonomous MicroED data. In mixture B, the relative volumes of the compounds were found to be consistent between the original composition and the MicroED analysis, with only slight deviations for some compounds. The RatioInp represents the volume ratio (%) for each component in the original composition, while the RatioObs was calculated by dividing the ratio of the total area of one component to the total area of all crystals in the MicroED analysis. Overall, the results demonstrate the feasibility and accuracy of using MicroED data for composition analysis of complex mixtures.