The original SCoPE MS method had a fundamental limitation in the number of cells that can be analyzed as well as the number of proteins that can be detected across all the cells in a given study. There are two fundamental challenges to a successful study where cell heterogeneity are the protein level must be resolved -depth of the proteome coverage and the consistency of protein abundance measurements across all studied cells. These two factors determine the amount of protein that can be used for the final analysis. The amount of carrier channel is usually around 100 cells, which typically provides several hundred proteins that can be detected across all cells in the study. Several techniques like DO-MS and DART ID were developed to overcome these issues and increase the total number of proteins that were consistently detected in such studies. We believe that a greater number of cells in the carrier channel can serve to improve the stability of large number of protein detection across all cells in a given study. A direct increase in the carrier channel shows that after about 200 cells the original SCoPE technique starts losing peptide identification rates and shows a decrease in the total amount of proteins that had been detected across the study due to high peaks of TMT label from the carrier channel. To overcome this issue, we proposed to move the carrier proteome labeling channel from the 131 position to the 126 position and to remove this peak from MS2 spectra detection to improve spectra quality and create an environment where more MS2 spectra can be identified across many more single cells. To implement this feature, we simply acquire all MS2 spectra with a QE HF-X Orbitrap instrument starting from fixed mass of 126.5 m/z. This type of acquisition allows us to detect all channels except the carrier channel itself.