PfCLK3 kinase domain (residues 334-699 with a C-terminal TEV cleavage sequence and His6tag) was cloned into pFastBac vector and expressed and purified from Sf21 insect cells. Cells were infected using P2 BIICs at an MOI of 0.2 and left to express for 72 hours. Harvested cells were lysed and centrifuged before purifying using IMAC and SEC in a final buffer containing 20 mM HEPES pH 7.4, 150 mM NaCl, 1 mM TCEP and 1 mM MgCl2.