2D carbon nanomateials such as g-C3N4 and GO were reported for repairing of bone defects in rabbit femurs . Biological analysis of osteogenesis was performed using X-ray, computed tomography (CT) and qPCR analysis. The expression meeasurment for osteocalcin (OC) and osteopontin (OP) were also included. Based on the data analysis, g-C3N4 exhibit amount of OC and OP leading to bone defects repairing. Graphene can be used as a surface for SALDI-MS .