Kibron Microtrough G2 with a surface area of 280 cm 2 was utilized for floating layer studies. Langmuir trough is made of hydrophobic Teflon to maintain subphase meniscus and prevent interaction to deposited specimen. Trough barriers are made of hydrophilic polyacetal to allow for compression process. Surface pressure was calculated from surface tension as measured with Wilhelmy microbalance with platinum plate. Water for subphase was bidistilled and deionized, to achieve (at least) 18MΩcm resistivity. Experiments were performed in the ambient conditions (in air atmosphere at 20±1 • C) BODIPY-C18 was deposited as 130µl of CHCl 3 solution with 4.45•10 -4 mol/L concentration on a deionized water subphase.