AmpC and D4. We evaluated the retrospective applicability of RAD in large-scale virtual screening using two protein systems. We obtained results for 99,459,562 molecules docked to AmpC β-lactamase (AmpC) and 138,312,677 to D4 dopamine receptor (D4) using DOCK from Lyu et al. We obtained Glide docking results for the same molecules and proteins from Yang et al. DUDE-Z. To assess the reusability of the HNSW structure across many protein targets, we created a dataset of 1,169,461 molecules from the DUDE-Z "Goldilocks" set docked to the 43 proteins included in the DUDE-Z dataset 36 using DOCK 3.7. We used the parameters, INDOCK files, and grids provided by the DUDE-Z dataset for each protein. Molecules that failed to dock to a given protein were assigned a score of infinity for that protein. On average, 89% of the molecules per protein were successfully docked and scored, each exploring 5,226 orientations and 294 conformations.