In order to more clearly explore and evaluate the performance of BLUES as compared to MD given the observed backbone remodeling, simulations of T4 lysozyme were run using different snapshots from the microsecond trajectory as starting points. The first set was run using the initial input coordinates for the system (time 0). The next set commenced from the 250ns point, just after the observed backbone transition. And the last set of simulations was run using a snapshot from the end point of the microsecond simulation. Hereafter, these starting points will be referred to as T0, T250, and T1000, respectively. For each starting point, 60ns of classical MD data was generated such that the trajectory frames were written with the same frequency as BLUES; this was important for ensuring that no fast transitions were missed as a result of differences in reporting frequency (as could have also affected the prior comparison for the microsecond simulation).