However, deviations from this pattern are common when comparing i) members of different families (e.g. adrenergic 5-hydroxytryptamine receptor 1B (5HT1B) and nucleotide adenosine A1 receptor (AA1R) in their overall dynamic behavior), ii) members of the same family but different subfamilies (e.g. nucleotide receptors adenosine A2A (AA2AR) and P2Y purinoceptor 1 (P2RY1) in TM2, ICL2, ECL2, ICL3, and C-terminus), or iii) even members of the same subfamily (e.g. 5-hydroxytryptamine receptors 5HT1B and 2B (5HT2B) in Nterminus, TM3, TM4, ECL2, ICL3, and ECL3). The observed similarities and differences in dynamic behaviors between GPCRs were effectively captured by the optimized rs3DDPDs (Figure and Figure ) and ps3DDPDs (Figure and Figure ). While rs3DDPDs reflected the dynamic fluctuations on a residue level that resembled more closely the RMSF pattern itself, ps3DDPDs showed a more generalized embedding of each protein dynamics compared to all the targets in the set thus enhancing the differences among targets. Of note, rs3DDPDs did not represent merely a transform of the RMSF values, as exemplified for the positions corresponding to the Nterminus and TM1 in P2RY1 and P2RY12 (Figure ). This suggests that information other than the atom coordinate variability, such as the type of atoms and residues encoded by partial charges, were picked up by the 3DDPDs.