For each of the three tested datasets (intact protein, peptide, and glycan data), IsoMatchMS calculated, matched, and visualized isotope profiles in under 90 seconds (Table ). Datasets where molecular formula information was already calculated from a theoretical database (glycans) completed faster than formulas derived from experimental ProForma 17 strings. Across all datasets, highly confident annotations derived by using other tools were also identified by IsoMatchMS at a Pearson score threshold of 0.7 (Table ). Even at higher Pearson score thresholds of 0.9 and 0.95, there was high overlap between the number of manual and IsoMatchMS annotations. An example of the trelliscope display output for the intact protein data was provided (Fig. ). While IsoMatchMS was able to annotate several dozen high-confidence isotopic profiles within the presented datasets, the number of matches per number of molecular formulae searched (Table ) was relatively low. This was due to the reduced dynamic range of MALDI-MS detect several dozen to a couple hundred species in comparison to LC-MS/MS which can routinely detect several hundred intact proteins or several thousand digested peptides.