Ag@C NPs display a particle size of 1-5 nm with an average particle size of 2.5 nm. The nanoparticles were embedded into carbon. Ag@C NPs were investigated as antimicrobial agents in bacteriospermia of fresh semen collected from five fertile bulls. They exhibited high antibacterial activity against bacteria species found in semen such as Escherichia coli (E. Coli),