Our results show that the RuAAC reaction can work even in crude extracts of biological samples, and that the triazoles formed by RuAAC are detected with electrospray-based mass spectrometry with much higher sensitivity than the parent alkynes. Our protocol does not require any anoxic technique (e.g. Schlenk lines) or anhydrous conditions, and thus could easily be adopted by most laboratories capable of electrospray-based LC-MS analysis. The primary limitation of the technique is that yields are low (less than 10%), probably because we did not employ anoxic techniques. Nonetheless, we found the technique as we performed it could improve the electrospray response of internal alkynes by up to 32,000-fold.