In each case, the guest was in the cell and the host in the syringe. The concentrations of the solutions were tailored for each complex, based on binding affinity, to obtain appropriate C values . Host concentrations ranged between 7 and 10 mM, and guest concentrations ranged between 200 and 500 μM, below their respective critical micellar concentrations . The raw data were analyzed with Microcal Data Analyzer in Origin 7.