China). Additionally, Ba/F3 cell lines stably expressing HRAS(WT) and HRAS(G12C) or NRAS(WT) and NRAS(G12C) were used. Cell culture and CellTiter-Glo assays using sotorasib and adagrasib were performed according to manufacturer's instructions (Kyinno biotechnology and Promega). Briefly, cells were cultured in 10% FBS supplemented RPMI-1640 medium and seeded in 96-well plates (3,000 cells/well). Cells were cultured overnight prior to sotorasib and adagrasib (0-10 µM) addition, and plates were further incubated for 72 h. Cell viability was measured using a CellTiter-Glo assay according to manufactures instruction by measuring the luminescence signal (Envision, PerkinElmer, Waltham, MA).