Interestingly, monoolein cubosomes gave considerably higher % loading efficiency of this hydrophilic dye than phytantriol cubosomes and phyt./toc. acetate hexosomes. The Pn3m phytantriol particles showed minimal loading efficiency. Hexosomal particles, while present at the same lipid concentration and with a similar size distribution, encapsulated dye concentrations below the dynamic range of the standard curve. This result could be attributed in part to differences in lattice parameters/phase behaviour, with water channel volumes expected to follow the trend: Im3m cubosomes >> Pn3m cubosomes > p6mm hexosomes.