We found that, for the 1HP origami-pso-TFO complex, the patterns of the Cy5-labeled products, their mobilities, and the kinetics of their formation were essentially indistinguishable from those in the minimal 1HP junction-pso-TFO complex (Fig . The observed efficiencies of the bis-adduct formation increased for irradiation times of 1, 3, and 9 seconds (74%, 80% and 81%, respectively), while the efficiencies of the intermediate mono-adduct formation decreased for the same time points (10%, 4%, and 3%, respectively). The same crosslinking patterns and kinetics were observed when we repeated the 1HP origami experiment with pso-TFO-PEG5K (Fig ), except that the mobilities of all detected adducts was shifted accordingly to the extra molecular weight of PEG5K, indicating that the bulky PEG5K residue did not interfere with the efficiency of pso-dependent crosslinking in origami. Crosslinking of 1HP-pso-TFO complexes prepared by the one-pot method followed the same kinetics as crosslinking of 1HP origami saturated by pso-TFO (compare 1HP origami gels in Figs and). In all cases, the pso-TFO-dependent crosslinking of 1HP was highly specific as no significant amounts of adducts of sizes bigger than the expected bis-adduct were detectable on the PAGE gels.