Dimethomorph, a commonly used fungicide in grape cultivation against mildew, was detected in almost all the wines except for two, one of which was produced in 1991 before the commercialization of dimethomorph. Figure To characterize the quantification performance of the approach, the concentration levels in wine samples were estimated using the single-point calibration of XIC of the TOF experiment using intensities ratios analyte/IS of standards spiked in pesticide-free red and white wine. Using 80 µL injection volume of sample the estimated concentration from the 27 pesticides detected ranged from 75.6 pg/ml to 35.4 ng/ml (Table ). For neonicotinoids, the estimated concentrations were 12 ng/mL for acetamiprid and 2.8 ng/mL for imidacloprid. LOD values were calculated for all pesticides using TOF MS signal intensities and CID, EAD, and UVPD, single fragment (Table ). The results show that TOF MS and TOF MS/MS exhibit similar LOD down to the picogram/ml level but are compound dependent. For boscalid, the CID LOD was higher than for EAD or UVPD, showing the selectivity and sensitivity benefits of using different fragmentation techniques.