Red arrows depict fuel-driven activation reactions. Blue arrows depict spontaneous deactivation reactions. B) In chemically fueled, dynamic self-assembly, the activated building block should assemble while the deactivated building block disassembles. C) In kinetically trapped assemblies, disassembly does not follow deactivation. Building blocks get reactivated in the assembly. D) Transient negation of charges by the reaction cycle can be applied to drive self-assembly. EDC-driven reaction cycle negates the charges on a dicarboxylate.