All the selected protein-peptide complexes underwent energy minimization using the Rosetta macromolecular modeling suite, version 2022.16 . The coordinates were refined using the FastRelax protocol. The protocol was used with the latest scoring function in cartesian space (-beta_cart flag) with the default repack and minimisation cycles parameters and a cutoff distance of 9.0 Å. A ramp-profile of 0.02, 0.25, 0.550 and 1.0 of the repulsive weight was used. At each step a repack is followed by a minimisation with a tolerance of 0.01, 0.01, 0.01 and 0.00001 respectively. The lbfgs_armijo_nonmonotone minimizer was used for the energy minimisation. Coordinates were constrained to the starting pose during the relaxation. In total, the energy minimisation was repeated 20 times for each protein-peptide complex, and the model with the best scoring pose was selected.