The time to detection of the lateral flow assay was also evaluated to determine its utility in clinical settings. This was conducted by monitoring the duration of time taken for the lateral flow assay to produce a detection band at a predetermined streptavidin region of interest. Using our experimental set-up, a detection band was observed in < 60 s (Supporting Movie 1). This is well within the time frame of a typical doctor-patient consultation (~10 minutes), providing sufficient time for a thorough clinical evaluation and sample extraction , prior to the implementation of a gold-aptamer-nanoconstruct enteroviral assay. Furthermore, in order to establish an unambiguous interpretation of the gold-aptamernanoconstruct based lateral flow assay, which could be used by both healthcare professionals and patients, a bespoke software and hardware solution was engineered. The desktop detector was composed of a light emitting diode (LED) and light dependent resistor (LDR), positioned perpendicular to a printed circuit board, separated by an opaque light barrier (Figure ). The light barrier only permits reflected light to be sensed by the LDR, therefore, in the presence of an enteroviral positive lateral flow membrane (Figure ), when compared to enteroviral negative membrane (Figure ), relatively less light was reflected and consequentially sensed by the LDR. The difference in light reflectance corresponded to a change in electrical resistance. A central processing unit was programmed to threshold the change electrical resistance, such that an user interface instantly displayed "POSITIVE" (Figure ) or "NEGATIVE" (Figure ) in the presence or absence of conserved enteroviral nucleic acid (Supporting Movie 2).