Dissolution DNP has proven a useful tool to study fast, kinetic reactions in real time. Thus, in this work, we provide the first direct and quantitative evidence of selectivity of yeast G6PDH towards the β anomer of G6P. Moreover, we have shown that it can be quite robust and provides satisfactorily reproducible results. In this regard, only a few time-resolved studies of chemical or enzymatic reactions by dDNP provide estimates of the experimental errors on kinetic parameters. In ref , Lee et al. extracted a pseudo-first order kinetic constant of the anionic polymerization of styrene based on three different measurements, and found errors comparable to ours. However, to our knowledge, no error estimates on kinetic parameters are provided in the context of enzymatic reactions.