Validation, Example -2, amino acids substituted by deletions at various positions Output: 1/18/2024 5:18:30 PM order as shown in Table . Basically the Segment-1 resolved into three unique groups or variants. The Segment-2 also resolved into three unique groups or variants. The Segment-3 however formed only one group. Different regions of the protein molecules mutating at different rates is not uncommon.