Specific activities of selected HheG variants (wild type and M45F) in the dehalogenation of 2chlorocyclohexanol (2e) were determined by halide release assay as described previously. Reactions were performed in 1 mL 25 mM Tris•SO4 buffer, pH 7.0 containing 20 mM 2e and 400 µg biocatalyst at 22 °C. Samples were taken after 30, 80, 180, 270 and 360 s. Specific activities were calculated based on released halide ions. Reactions were performed in duplicate. Chemical background dehalogenation in reactions without enzyme addition was substracted.