Propachlor treatment has the strongest effect on the DNAJB8 H31Q -associated proteome (Figure and Figure ). The two most prominent targets that are unique to propachlor are glyceraldehyde-3-phosphate dehydrogenase (GAPDH; fold change = 5.97, q-value = 6.09 x10 ) and Parkinson's disease protein 7 (PARK7/DJ-1, fold change = 3.8, q-value = 5.56 x 10 -6 ). GAPDH is an enzyme that canonically uses a susceptible active-site cysteine to bind and reduce nicotinamide adenine dinucleotide (NAD) in glycolysis , but consistent with its high abundance also engages in extensive moonlighting activities . Due to its high abundance and pK-perturbed active site cysteines, GAPDH is a frequent conjugation target of electrophilic molecules .