Having easily prepared the triazoles, they were then deprotected by standard Zemplén deacetylation with fresh 0.1 M NaOMe solution to obtain pure ligands 1 as white solids upon drying. They can also be precipitated from EtOH. All deprotection reactions resulted in high yields (>95%) at a scale in the hundreds of milligrams, with facile isolation, which makes them convenient synthetic targets. Complete disappearance of the characteristic ester C=O band in IR spectrum of 1Gal compared to 1Gal OAc confirmed deprotection (see ESI †), alongside absence of the characteristic acetyl CH3 resonances in 1 H NMR spectra. The 1 H NMR spectra of each glycoconjugate ligand shows a single set of peaks, corresponding to the formation of single anomers without epimerisation, evidenced by coupling constants of the anomeric proton resonances at of 9.2 Hz for 1Gal and 1Glc, and 9.2 Hz (lactoside glucose moiety) and 7.8 Hz (lactoside terminal galactose moiety) for 1Lac respectively. The lactoside is linked to the triazole via the glucoside anomeric position thus making it more de-shielded than the galactoside moiety. High resolution mass spectrometry (ESI+, Q-TOF) was used to further characterise the ligands and in all cases the mass found agreed with the calculated m/z of the ion. For instance, for 1Glc, calculated for C25H33N9O12 + [M+H] + m/z = 652.2326; found m/z = 652.2320.