The EC50 range for each of the toxicants tested was determined based on the relationship between its concentration and the calculated 𝐼 𝑟 value., To determine the exact EC50 values, the experiments were replicated for each of the toxicants using the same methodology as described above, but different series of decreasing concentrations based on the EC50 ranges found for each compound: 2.84, 2.13, 1.60, 1.20, 0.90, and 0.625 mg dm -3 for ILs comprising a single anion, less compared to previous reports. The resulting bromide was isolated by filtration followed by washing with an apolar solvent, but originally used hexane was substituted by significantly safer heptane. These changes made it possible to reduce the negative environmental impact of the process. were obtained by mixing AB0.9 and B according to an analogous method. All of the obtained systems were colorless liquids at 25 °C. The formation of water during synthesis, combined with highly hygroscopic properties of ion exchange products, results in a high water content in the raw products (approximately 4-6%, Table ) and difficulties in its removal. Such a high water content would significantly affect and alter analysis of the physicochemical properties of the obtained DSILs (e.g., density, refractive index). Therefore, an effort was made to remove most of the water from the DSILs via a two-step azeotropic distillation. Thorough removal of solvents was facilitated by subsequent drying of the residue using a Schlenk line with a vacuum of approximately 2•10 -5 bar.