The plasmids were diluted to 75 ng/μL in DNAse free water to a total volume of 15 μL. Each sample was prepared in duplicated to which 2 μL of either CMV (forward primer) or BGH (reverse primer) was added. Sequencing was performed by Eurofins. The nucleotide sequence was obtained in a FASTA file. For the data obtained from the reverse primer the forward sequence was obtained using a reverse complement tool. All forward nucleotide sequences were translated using the Expasy translate tool. The blastp tool was used on all translated sequences. Selected transformants were scaled up and the plasmid DNA purified using a NucleoBond Xtra column (Macherey Nagel, Germany).