Besides demonstrating the capabilities of the models for the three LNPs, we also inserted the LNPs into a lipid vesicle representing the same membranes studied in the PMF fusion assays of Section 2.3. Two different membrane models were used: one plasma membrane model without charged lipids in the interacting leaflet, and another, called endosomal membrane model, with 10 mol% of PAPC lipids replaced by negatively charged PAPS.