The HILIC LC method (Table ) appears to be the most suitable method for future non-target experiments. Since most of the enriched clusters (Figure ) are highly hydrophilic (logP < -1), an expansion of the non-target methods to explore the hydrophobic part (lipidome) of the CSF could reveal extra information in future efforts. Overall, while MS-DIAL provided a higher number of annotated chemicals, the combination of different software and suspect lists enhanced the annotation of a variety of chemicals, increasing the general understanding of the CSF metabolome/exposome. Furthermore, the identification of some chemicals in this study (e.g. galacturonic acid, threonic acid, N-acetylhistidine, and some of the BAs) could help expand the current HMDB-CSF database, as they are not yet included in this resource.