Cyclic AMP was manually docked to the active conformation of EPAC1 by aligning the EPAC2 complex with the cAMP analogue (Sp-cAMP) in the PDB entry 3CF6, and editing it with the software Flare. cAMP was further manually docked to the inactive conformation of EPAC1 by aligning the cNBD regions of the protein using PyMol and copying the ligand coordinates.