Within the scope of our preliminary work, a series of 11 structurally diverse SSAs were assessed for antibiofilm activity against biofilms of clinically relevant Gram-negative P. aeruginosa and fungal pathogen C. albicans. From these proof-of-principle studies, 2 demonstrated the greatest activity against P. aeruginosa biofilms, while 1 and 3 exhibited the greatest activity against C. albicans biofilms. When conducting preliminary structure activity relationship analysis, the data lead us to hypothesise that increased SSA anion self-associative hydrogen bonding strength was a contributing factor to increasing antibiofilm efficacy. Building on the results from this proof-of-principle study we now explore the effects of combining multiple SSAs (Figure ). In addition, we also establish the effects of increasing SSA anion