The AFM measurements were conducted with an Asylum Research Cypher ES instrument (Oxford Instruments Co, Ltd). The mechanical testing was conducted in Fast Force Mapping mode with HQ:NSC15/ Al BS (MikroMasch), RTESPA-300-30 (Bruker), and biosphere B300-NCH (nanotools) cantilever probes. To ensure accurate measurements, the tip radius was confirmed by a blind tip reconstruction method using calibration standards. We used a TGTZ-4000 calibration specimen (Ted Pella) for the B300-NCH probes, and a titanium roughness sample (RS from Bruker) for the other probes. The spring constant was calibrated using the v4 GetReal TM function of AR software (Oxford Instruments, Co, Ltd). The nominal tip radius Rbox, nominal spring constant kbox, and nominal fundamental eigenfrequency fbox of each cantilever type are summarized in Table along with our own measurements of the fundamental eigenfrequency f, fundamental quality factor Q, static spring constant via GetReal k, and probe radius R.