Our approach holds promise for the bio-engineering field of artificial cells. Dynamic topological changes such as fusion and fission are essential functional processes in living cells and hence are highly desirable features to engineer into minimal artificial cells. To enhance this approach for the engineering biology toolbox, the leakage of GUV contents should be minimised to prevent loss of vital functional biomolecules from the GUV lumen, and further regulation of the interactions between LCNPs and GUVs might direct the morphological and topological transitions towards a specific mechanism (e.g., fusion, fission, ILV formation).