The clipped grids were loaded in an aligned Thermo Fisher Talos Arctica Cryo-TEM (200 kV, ~0.0251 Å) at 100 K, equipped with a Falcon III direct electron detector (4096 × 4096 pixels). Intensity of 45.2% was found to be the condition for parallel beam during diffraction using the contrast of the objective aperture. For diffraction movies the data was collected with an 829 mm diffraction length and using the smallest C2 aperture of 20 µm without the selected area aperture. The resulting beam size was approximately 1.5 µm. MicroED data was automatically collected using the SerialEM software in microprobe mode.