We performed an all-against-all comparison of the KLIFS IFP describing interactions between co-crystallized ligands and the KLIFS pocket. For each pocket residue, seven potential protein-ligand interaction types were defined as described by Marcou and Rognan . The presence or absence of a certain type of interaction is noted as 1 or 0 in the bit-string. This results in an 85 • 7 = 595-bit long IFP per pocket-ligand pair. The Jaccard distance is used to compare the IFPs. If multiple IFP pairs describe the same kinase pair, we selected the minimum distance as the representative measure for the kinase pair, following the same procedure as described for the KiSSim methodology.