As would be expected given the pK a of SA, SA-amine nucleation at 263.15 K is significant, reaching values above 1 cm -3 s -1 at a SA concentration of 10 5 cm -3 with high amine concentrations, and at a SA concentration of 10 6 cm -3 with low concentrations. Noting the overestimation by 1-2 orders of magnitude due to the limited cluster sizes in the simulation, this means that we should have 10 2 cm -3 s -1 or more in the simulation to be certain of significant nucleation. At low temperatures, this threshold is reached at SA concentrations between 1 × 10 6 cm -3 to 3 × 10 6 cm -3 , depending on the amine concentration. At higher temperatures, this threshold is reached at a SA concentration of 10 7 cm -3 at high amine concentrations.