The structure of CISH in complex with Elongin B and C was predicted using Alphafold-Multimer 67 . The sequence of either the expression construct (CISH 66-258 Δ174-202, ElonginB 1-104 and ElonginC 17-112) or full-length CISH was used. The full database of reference sequences and structures was used with structures published after 01-01-2022 excluded and 5 seeds per model where generated. The best prediction by pLDDT was used for a 8inal relaxation step to improve local geometry.