In this article, we used computer aided design in Autocad, to directly imprint a desired electrode pattern on ITO coated glass surfaces with the help of a CO2-laser. The process was optimized to obtain electrodes with widths as small as 25 µm. The electrodes thus obtained were characterised using electrochemical methods and optical and scanning electron microscopes were used to identify the geometric parameters of the electrodes. A supplementary method was used for creation of larger area electrodes where the high resolution of direct laser cutting was not necessary, in which a stencil was prepared using the laser and the undesired ITO, outside the electrodes was removed by wet-etching. This method is much more efficient than direct cutting of big areas using the laser, but suffers lower resolution. Both methods together form a complete toolbox, which allows for low-cost and fast fabrication of ITO electrodes for wide variety of applications.