Experimental spin-spin coupling constants (J-couplings) measured by NMR spectroscopy are widely used to determine the structural properties of saccharides in solution. J-Couplings are sensitive to valence bond angles, bond torsion angles, and bond lengths within and adjacent to the coupling pathway. Vicinal (three-bond) J-couplings ( 3 J ab ) depend strongly on the torsion angle between the two coupled nuclei, and experimental values can be interpreted quantitatively via Karplus or Karplus-like relationships, revealing conformational preferences in solution. Geminal J-couplings ( 2 J ab ) are used in structure determinations less frequently, and are affected by the configurations of the terminal coupled carbons and by the configuration of the central carbon. In the latter regard, J CCC values depend strongly on the rotameric properties of the C-O bond involving the central carbon, and less so on the rotameric properties of the C-O bonds involving the coupled carbons. Direct (one-bond) J-couplings ( 1 J ab ) have a strong dependence on bond length, with larger values corresponding to shorter bond lengths (greater scharacter). Despite suggestions that 1 J values could serve as valuable structural constraints in saccharides, detailed conformational interpretations of them have proven challenging. Experimental determinations of saccharide conformation often rely on computational methods such as density functional theory (DFT) or molecular dynamics (MD) simulations. Current approaches combine experimental parameters with appropriately restrained calculations to derive a model that best matches the experimental data. While this approach has proven valuable, it is not without limitations stemming from limited experimental validations of the computational methods. The structural dependencies of experimental parameters may not be understood quantitatively and/or the quantity of experimental data used to derive a conformational model may be limited, placing greater reliance on the model obtained from calculations. X-Ray crystallography and solid-state C NMR spectroscopy have been used synergistically to determine the structural properties of carbohydrates in solution by addressing some of the limitations discussed above. MA'AT analysis, a new experimental method to model molecular conformation in solution, combines DFT calculations and experimental NMR J-couplings to give probability distributions of molecular torsion angles in solution. MA'AT probability distributions are superimposable on those obtained by MD simulation, allowing direct comparisons of experimental and calculated models. The method uses 2 J and J values primarily to model various conformational elements in saccharides (e.g., O-glycosidic linkages, ring conformation, side-chain conformation ). To extend MA'AT analysis to multi-state models, it would be useful to employ J CC and J CH values as inputs due to their high abundance in saccharides and their attractive conformational properties (e.g., large dynamic ranges).