In conclusion, the present work manifests the role of cyclopropane rings towards the survival of E. coli bacteria during cold shock. We have simulated a realistic lipid bilayer of an E. coli bacteria along with the modified membranes, replacing the cyclopropane rings either with single or double bonds in both CG and AA resolutions. The simulations are performed at various temperatures between 250 and 350 K. There is hardly any difference between the impact of cyclopropane ring and double bond over the structural and dynamical properties of the membrane in CG resolution, which indicates the insufficiency of the CG model of cyclopropane containing lipids to explain the membrane properties. Nevertheless, in all-atom resolution, we have obtained visible difference in membrane properties due to the presence of cyclopropane ring and unsaturation specifically at lower temperatures. Notably, the cyclopropane rings are more effective to restrict the closer packing of the membrane lipids compared to double bonds by providing a rigid kink along the acyl chain. In addition, cyclopropane rings not only prevent the fluid-to-gel phase transformation of the corresponding lipids, but also inhibits the phase transition of the unsaturated lipids as well by interacting with all different lipids present in the membrane. Therefore, this study provides an explanation on why the E. coli bacterial strain, susceptible to freezing environment, relies on the cyclopropanation of the acyl chain-double bond. Similar explanation may also be equally applicable for other bacteria, adopting cyclopropanation as cell-survival strategy.