Fa y acid profile by GC-FID. For fa y acid profile analysis, total lipids (TLs) were extracted from the microalgae according to the method described by Bligh and Dyer with small modifica ons. The extrac ons were performed in triplicate. Fa y acids methyl esters (FAMEs) were obtained in triplicate by acid-catalysed transmethyla on of dry lipids extracts using 2 mL of 1 % sulfuric acid in methanol and 1 mL of toluene to improve transmethyla on of non-polar lipids. To avoid oxida on, the tubes were filled with N2 gas, and then the reac on was performed at 50 °C for 16 h. A er transmethyla on, tubes were le at room temperature to cool down, and then 4 mL of hexane/diethyl ether (1:1 v/v) with BHT (0.01 % w/v) and 2 mL of KHCO3 (2 % w/v) were added to each tube. The tubes were vortexed, and the hexane phase was recovered a er centrifuga on at 2000 rpm for 5 min at 4 °C. The aqueous phase was washed with 4 mL of hexane/diethyl ether, and a er centrifuga on the hexane phase was collected and combined to the previous organic phase to be dried under a stream of inert nitrogen. FAMEs were resuspended in hexane and filtered through a 0.45 µm filter prior injec on.