TEM revealed distinct ultrastructural modifications within the parasite mitochondrion induced by 14 already after 4-6 h of compound exposure. Proteins binding to compound 14 were identified using differential affinity chromatography followed by shotgun mass spectrometry. These assays revealed that 128 proteins in T. gondii specifically bind to 14, the most abundant (26% of the binding proteins) being a protein with unknown functions annotated as YOU2. This protein is a homolog to the human mitochondrial import inner membrane translocase subunit Tim10. Additionally, the dataset contained proteins involved in key steps of metabolism and nucleic acid binding proteins. The fact that this compound binds to a considerable number of T. gondii proteins indicates that it could interfere in different cellular pathways.