The main objective of this analysis was to determine whether the 11 λ windows protocol used in the generation of RBFE-Space was sufficient to describe statistical fluctuations of RBFE perturbations. Although at n=11 convergence does not seem to have been reached in all cases, this number does offer a reasonable approximation of the statistical fluctuation with acceptable sampling cost. Notably, RBFENN SEM predictions consistently show the correct order of magnitude for all 12 perturbations described in this analysis. This confirms that the SEM estimator can be used to discriminate perturbations with high SF from perturbations with low SF.