To characterise the agonist pharmacology of the synthesised compounds, ability of the compounds to cause accumulation of cAMP in response to the ligands was measured. Compound 5 increased cAMP accumulation in the A 2A CHO-K1 (pEC 50 7.3) however, maximum cAMP accumulation levels were lower than that of NECA (E max 65%). This suggest that Compound 5 is a A 2A receptor partial agonist.