A compression-responsive degradable hydrogel was then generated by incorporating EGTAloaded vesicles into the alginate hydrogel. The EGTA concentration for the vesicle encapsulation was 880 mM. This concentration was chosen based on a simple experiment to check the required EGTA concentration for degrading the entire gel within a short amount of time (~20 min) (see Supporting Information, Figure ). Following encapsulation of EGTA in vesicles using cDICE, the final EGTA concentration within the vesicles was examined, as it is common that the inner solution of the droplets can be diluted by the outer aqueous solution during the bilayer formation. The EGTA concentration within the vesicles was found to be 844 mM, indicating an encapsulation efficiency of ~96% (Figure ).