Filamentous fungi from the genus Aspergillus are prolific producers of secondary metabolites which display a diverse range of bioactivities. As part of our ongoing chemotaxonomic exploration of rare and talented Australian Aspergilli, we recently reported the novel species Aspergillus burnettii, isolated from soil collected in Queensland, Australia. Chemical profiling of A. burnettii revealed a variety of unprecedented secondary metabolites, including the polyketide-peptide hybrid burnettramic acids, which displayed potent antifungal activity. Continuing the chemical exploration of A. burnettii, herein we report the structure, biological activity and biosynthesis of the novel polyene-decalin polyketide, burnettiene A (1).