The calibration curve and chromatograms for psilocybin standards are seen in Figures and. The run time is short and may feasibly be kept under 1 minute for psilocybin or 2 minutes for psilocybin and psilocin separation. Standards below the lowest concentration reported in the calibration were eliminated as outliers below our method limit of detection (2.10 ppm). The linear fit for this calibration demonstrated good agreement (0.985 Pearson R 2 ) for the concentration range used (2.53 -81.9 ppm).