Membright 647 (MB 647) labelling have been performed in OptiMEM by addition of 1 µL of MB647 solution in DMSO (100 µM) per mL of medium (final concentration 100 nM) and incubated at RT for 5 min before imaging. Lysotracker green (Invitrogen, thermofisher scientific) has been used according to supplier's protocol at 50 nM in OptiMEM with 5 min incubation time at room temperature before imaging. Hoechst 33342 (Invitrogen, thermofisher scientific) labelling has been performed at 1 µg/mL with 30 min incubation time in growth medium before applying other staining for live cells imaging or incubated with secondary antibodies for immunostaining experiments. Wheat Germ Agglutinin AlexaFluor conjugate labelling (WGA 488 or WGA 647, Invitrogen, Thermofisher Scientific) has been performed according to supplier's protocol in HBSS for 10 min at 37 °C at 5 µg/mL. For immunofluorescence staining, cells were incubated for 15 min with 4% (w/v) formaldehyde solution in phosphate-buffered saline (PBS) at room temperature. The fixative solution was removed and the cells were washed with PBS 3 times. Then, the cells were treated with 1 mL of 0.1% Tween-20 in PBS respectively for 10 min at room temperature. The permeabilization solution was removed and washed 3 times with PBS. Cells were blocked with 0.5 % solution of BSA in PBS for 2 h at room temperature. Then, primary antibody (H5C6 anti LAMP-3) solution in 0.5 % BSA in PBS has been added and cells incubated at + 4 °C overnight. Primary antibody were washed 3 times with PBS and then secondary conjugated antibody (Invitrogen AlexaFluo 647 conjugated anti mice) has been added with additional phalloidine AF488 conjugate at 100 nM and Hoechst 33342 and incubated for 2 h at room temperature. Cells were washed 3 times with PBS before imaging.