Analysis was performed using a Vanquish Horizon UHPLC coupled to a Q-ExacNve Plus hybrid quadrupole Orbitrap mass spectrometer with extended mass biopharma opNon (Thermo ScienNfic, Bremen, Germany). The system was controlled via Chromeleon CDS (Version 7.3.1) for data acquisiNon and data analysis. The intact workbook was created using Thermo ScienNfic Biopharma Finder (Version 4.1). SEC analysis was carried out using a 2.1 × 150 mm, 1.7 μm Waters Acquity Protein BEH SEC column (Waters CorporaNon, Milford, MA, USA). 50 µg of protein was injected onto the column and analysis was performed using isocraNc condiNons with 50 mM ammonium acetate in water at a flow rate of 300 µL/min. The column temperature was set to 30 o C. Analysis was performed in high mass range (HMR) mode, using 35,000 (at m/z 200) as resoluNon se}ng. The tune parameters were set to the following: a sheath gas flow rate of 20 au, an auxiliary gas flow rate of 10 au, a spray voltage of 3.8 kV, a capillary temperature of 300 °C, a vaporizer temperature of 250 °C and a RF lens se}ng of 200%.