XRD analysis of compounds 2-6 was performed to determine the molecular arrangement. Clear diffraction peaks were obtained for compounds 2-5 (Figure ), and these profiles suggested a with the lowest thermal energy, calculated using MOPAC in Chem3D software (PerkinElmer Software), was a compound in which the fatty acid chains attached to the 2-and 3-position hydroxy groups were in pairs, and the fatty acid chains attached to the 5-and 6-position hydroxy groups formed another pair, each extending in opposite directions. The α-position of the fatty acid chain attached to the 3-and 5-positions was twisted (Gauche), and the rest was in an all-trans structure. The calculated molecular lengths were 3.49 nm (C12GL (2)), 3.98 nm (C14GL (3)), 4.46 nm (C16GL (4)), and 4.94 nm (C18GL ( )). XRD results showed that for each compound, molecular lengths of 3.27 nm (C12GL