In the C NMR spectrum, seven separate regions are presented, as according to definitions used in previous reports Generally, information extrapolated from the C NMR spectrum of TRM-0522 agrees with the analogous 1 H NMR spectrum. Furthermore, the general regions and rough peak shapes correspond to previously reported spectra . While there are observable peaks in the aromatic/olefinic region, they are relatively minor when compared to those derived from saturated carbon functionalities. This is consistent with the relatively low content of lignin, humic, and fulvic substances in oceanic samples . A carbonyl signal is observed in the region typically associated with carboxylic acid and amide functionalities (~ 190 -170 ppm), while no detectable signal is associated with other carbonyl functionalities (ketones, aldehydes, carbamates, carbonates). In agreement with the 1 H NMR spectrum analysis, a strong band corresponding to the alcoholic carbons in sugar compounds (95 -75 ppm) is observed. While the anomeric signals are obscured by the residual water peak in the 1 H NMR spectrum, a peak attributable to the anomeric carbons of carbohydrates is observed in the C NMR spectrum (~ 105 -95 ppm). The remaining two regions contain strong aliphatic signals, with the signal reducing in intensity outside the 55 -15 ppm range. This is consistent with the presence of cyclic and linear terpenoid derived material, with cyclic material tending towards the higher chemical shift range, and linear material tending towards the lower chemical shift range .