In the presence of elongated guests, such as a long aliphatic chain, two OA hosts can encapsulate a guest forming a ternary complex. This phenomena is more likely to occur as polarity decreases for the groups at both ends of the guest . However, as described earlier in section 3.5, SAMPL7 was designed around systems which exhibit 1:1 binding. Isothermal titration calorimetry (ITC) experiments have shown that short-chain fatty acids, amphiphilic molecules, and large polarizable anions form 1:1 complexes , as do the guests reported here.