Erlymenyer flask (5000ml); measuring cylinder; Whatman No.1 filter paper; separator funnel; Heidolph rotary evaporator to concentrate filtered solution; Capillary tubes for TLC spotting; droppers; graduated cylinders; taste tube; refrigerator; TLC plates (20 x 20 cm); TLC chamber; UV lamp (254 nm and 366 nm) and vials samples. Column chromatography was performed using silica gel 60 (60-120 mesh) Merck; Mueller-Hinton agar (Merck, Germany) was used to investigate the antibacterial activity; Nutrient agar (Merck, Germany) medium was used for maintenance the bacterial strains; Micro titration plates (Citotest, China); Spots of compounds on TLC was detected using UV-254 nm and 365nm. Gallenkamp was used to measure the melting point of isolated compound. NMR spectra of the compound was recorded on a Bruker Avance 400 spectrometer in CDCl 3 operating at 400 MHz for 1 H-NMR; 100 MHz for 13 C-NMR and DEPT-135 with TMS as internal standard. Chemical shift values for all NMR data are reported in parts per million (ppm) relative to internal standard.