Considering the significant number of compounds included in each method, i.e., 83 in ESI-mode and 52 in ESI+, it is essential to optimize the total cycle time needed to acquire 1xMS 1 + 10xSWATH windows + 1xMS 1 + 83xMRM transitions. This is related to the fact that, as a rule of thumb, a chromatographic peak should have at least between six and ten data points in order to achieve satisfactory gaussian shape and reproducibility. Consequently, the above-mentioned acquisition cycle has to be repeated at least six times along the width of every chromatographic peak for proper quantification. Therefore, accumulation time for each of the mentioned experiments must be fine-tuned. The parameters found to be the most important are the accumulation time and the retention time window of each MRM transition, both due to the high number of overlapping MRM transitions.