As highlighted before, membrane properties such as formation of lateral domains or curvature, which all usually involve changes in cholesterol content, can well be studies with advanced microscopic techniques such as fluorescence, Raman or AFM, reporting e.g. on local membrane composition, fluidity and order, or curvatures. Recent advances in spatial and temporal resolution of these techniques have brought them closer to studying the molecular scale and sub-second and -millisecond fast processes, i.e. reaching the scales accessible by MD simulations. In this way, MD simulations can be fed as well as tested by information from experimental data, and experiments can be guided by insights from MD simulations . This of course accounts also for studying the molecular details of the interaction of AMPs with bacterial membranes, potentially opening new routes to the design of improved antimicrobial actions.