In another study, a multiplexed detection methodology for interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α) in saliva was developed by Zhang et al., using silicon nanowire (SiNW) fieldeffect transistor (FET) biosensors. The SiNW FET sensors were fabricated using a top-down lithography fabrication technique, and specific recognition of the two biomarkers with their corresponding antibodies modified on the SiNW allowed for the multiplexed detection of the two biomarkers in saliva. The established method had a low limit of detection of 10 fg/mL in 1×PBS and 100 fg/mL in artificial saliva, making it suitable for the early diagnosis of oral squamous cell carcinoma (OSCC). The label-free and multiplexed detection approach of the proposed method makes it highly sensitive and specific for the non-invasive analysis of OSCC.