Metabolite profiles produced by the methods described herein, illustrating the field of metabolome capture across chemical space from smaller, polar, and ionic molecules (HILIC) to larger and more moderately hydrophobic molecules (RPC small molecules) and lipid species (RPC lipids). Two-dimensional feature maps (x = chromatographic retention time; y = m/z) show noise-filtered data (using the previously defined criteria of feature precision and correlation to dilution ) from selected studies where urine (blue features) and blood products (red features) were analysed. LogP values were calculated using the RDKit, an open-source cheminformatics toolkit ().