The use of automated synthesis also avoids the requirement for large-scale aqueous workups and purifications. Synthesis of the library under classical conditions would require up to 3732 chromatography stages, which when combined with the work-up stages results in a total solvent usage of 4740 L. In contrast, the automated protocol avoids the requirement for purification, with an estimated total solvent usage of 5-15 mL (Table ) per analogue. This would require a total of 18.7 L of solvent, an over 250-fold reduction in costly and potentially polluting solvent usage.