Research into two-dimensional (2D) materials has evolved in the last ten years from the labscale proof of concept to pilot-scale demonstrations. One of the key challenges in converting the prototype to a product is the energy cost of 2D materials' production; since all of the material synthesis uses external energy sources such as electricity, chemicals, mechanical force etc., and limits to decrease the cost of the production. However, there are several biological processes wherein nature produces compounds without the aid of external energy. For example, collagen in living species is produced without any external energy source. Previously, there have been attempts to mimic such processes using yeast for the production of chiral centres etc. However, such self-mediated natural processes have never been explored to produce 2D materials. Here, we report the self-exfoliation and functionalization of graphene by using yeast in the presence of glucose without any external energy source. We show that, in such a process, the yeast cells exert much-needed mechanical shear forces to exfoliate graphite from the edges.