However, we acknowledge that this interpretation is specific to the used assessment criteria and penalty scores. Other advantages of biological reactions such as greenness and close-to-ambient reaction conditions have not been covered by the current methodology. We also did not implement any quantification of the drawbacks of biological reactions. For example, it is common for biological reactions to be highly dependent on the rest of cellular metabolic network, which increases operational costs of reactions. We also did not consider product separation for any of the reactions in the current implementation.