Full-length capsid protein separation of AAV2 was performed using an Acquity UPLC Glycoprotein BEH Amide Column, 300 Å, 1.7 µm, 2.1 × 150 mm (Waters Corporation, Milford, MA, USA). One microgram (1 µg, 8.75 µL) of AAV2 sample was injected neat, with triplicate injections performed. AAV capsid separation was performed following a modified version of the LC parameters outlined by Liu et al. for VP separation . They were as follows: At a flow rate of 0.100 mL.min -1 , an isocratic hold at 85% mobile phase B (85% B) was performed for 3.500 min followed by a decrease to 64.5% B over 0.100 min. An Isocratic hold was performed for 7.067 min followed by a linear decrease in mobile phase B from 64.5% to 58.5% over 21.323 min. A column wash was then performed by decreasing mobile phase B to 5% over 1.000 min while simultaneously increasing the flow rate from 0.100 mL.min -1 to 0.260 mL.min -1 . A hold at 5% B with a solvent flow rate of 0.260 mL.min -1 was performed for 3.500 min. Column re-equilibration followed by increasing mobile phase B to 85% over 0.500 min at 0.260 mL.min -1 and holding for 2.000 min at 85% B before increasing the flow rate to 0.400 mL.min -1 over 0.000 min. The flow rate was held for 2 min at 0.400 mL.min -1 before being decreased to 0.100 mL.min - 1 over 0.000 min. The flow rate was held for 4.000 min at 85% B to ensure complete equilibration before next injection. The column temperature was kept constant at 25°C, 45°C, or 60°C, dependant on which column temperature was being tested during a run. Mobile phase A was UHPLC-MS grade water containing 0.1 % (v.v -1 ) DFA and mobile phase B was Optima™ LC-MS grade ACN containing 0.1 % (v.v -1 ) DFA.