PLT viability after incubation and exposure to IONFs was also assessed through flowcytometry. A control group was considered along with samples at four different IONF concentrations, and incubation times of 30 minutes, 3 hours, and 6 hours. expected, analysis of the control solution indicated no pseudopod formation. For this reason, and in agreement with previous studies on PLTs, the side formation in the scatter plots was associated with morphologically changed platelets (i.e. activation induced by IONFs).