Analysis of simulation trajectories was carried out within Maestro. The trajectory density profiles were created using the Trajectory Density Analysis module by averaging the density profiles of each component from the trajectory snapshots over the last 20% of the total simulation. Tilt angles for covalently attached 18-MEA fatty acids were computed using the Surfactant Tilt Calculation Panel. We define a vector pointing from the carbonyl group of 18-MEA to the last carbon on the end of the chain furthest from the surface and measure the tilt angle between this tail vector and the plane defined by the hair surface. To account for symmetry of both surfaces in the simulation cell, only the smaller of the two possible complementary tilt angles were reported. To calculate the spacing of covalently attached 18-MEA fatty acids during the course of each simulation, radial distribution functions were generated over the last 500 ns of each simulation using the sp 2 carbon of the thioester linkage.