N-terminal clipping was observed at 30-50% reduced levels for the peptide map MAM workflow, which suggests that clipping occurs on the N-terminal of one chain of the GLP-Fc fusion protein. Using this hypothesis, analysis via intact MS should yield levels of clipping for both the removal of the H and HG approximately 50% higher than that observed for the peptide analysis. The levels of clipping for both the N-terminal H and HG clippings at the intact level were 66 % and 62% higher compared to that observed via peptide map analysis. The results for the four commercial DP batches and drug substance