Low dose of 1.74 X 10 4 particles cm -3 ; medium dose of1.27 X 10 5 particles cm -3 ; high dose of 1.32 X 10 6 particles cm -3 of AgNPs size 13-15 nm Inhalation exposure for 6hrs a day, 5 times a week for 4 weeks Size and number of Goblet cells containing neutral mucin increased. However, no remarkable changes in nasal and lungs canal. Sprague-Dawley rats Low dose of 0.7 X 10 6 particles cm -3 ; medium-dose of 1.4 X 10 6 particles cm -3 ; high dose of 2.9 X 10 6 particles cm -3 of AgNPs size 18 nm Inhalation exposure for 6 hrs a day, 5 times a week for 13 weeks Lung inflammation and decrease in tidal volume and minute volume. Sprague-Dawley rats Low dose of 1.74 X 10 6 particles cm -3 ; medium dose of 1.27 X 10 6 particles cm -3 ; high dose of 1.32 X 10 6 particles cm -3 of AgNPs size 12-16 nm Inhalation exposure for 6 hrs per day,5 times a week for 4 weeks Both male and female rats show no significant changes in the body weight, hematology and blood biochemical values. Sprague-Dawley rats Low dose of 30 mg kg -1 ; medium dose of 300 mg kg -1 ; high dose of 1000 mg kg -1 of AgNps size 60 nm Ingestion exposure in which the AgNPs are mixed with diet for 28 weeks Gender related difference in accumulation of AgNPs in kidneys; significant dose dependent changes in alkaline phosphate, cholesterol values. Toxicity in non-mammals Numerous non-mammals are been considered and used to test the adverse effects of AgNPs. It is not a surprise that majority of these tested and adversely affected non-mammals are aquatic organisms as a large quantity of AgNPs are released from fabrics and textile would flow into aquatic system. Few non-aquatic non-mammals such as Fruit Fly (Drosophila melanogaster) are studied. Zebrafish has been used as a correlative and predicted model in many studies to evaluate thre effect of AgNPs . A study, in which embryos of Zebrafish were exposed to a dose concentration of 0.04-0.71 nM for 120 hours. It was noted that single AgNPs of size 11.6±3.5 nm could transport into the Zebrafish embryos through Chorion pore canal, AgNPs were detected in each development stage. At a concentration of 0.19 nM development abnormality could be triggered . Another study consisted of synthesizing four different size of nanoparticles of size 3 nm, 10 nm, 50 nm, and 100 nm respectively, which were then tested on Zebrafish embryos to test its toxicity, only a few differences were observed between them. It was reported that AgNPs induced 100% mortality when exposed for 120 hours at 250 µM. At a dose of 100µM, variety of embryonic morphological deformations were reported . In another study the author reported that larger AgNPs of size 41.6±9.1 nm were more toxic and produced sever deformation of Zebrafish than smaller AgNPs of size 11.6±3.5 nm .