Jansen et al. have developed a tool for the analysis of high-pressure liquid chromatography (HPLC) measurements termed as 'HappyTools', able to calibrate retention times, perform peak quantitation, and use various quality criteria to curate the compiled data. For the quantitation and calibration of the chromatographic peaks the user can either input a peak list containing the time retention time and retention time window of the target chemicals, or it can use an automated peak detection algorithm removing the need of user input. The peak detection algorithm was developed using a loop to attain the user-specified cut-off value of the highest intensity peak. A new univariate spline is fitted for each iteration, from which the local maxima and minima are determined. Overall, HappyTools showed similar or better performance in comparison to existing commercial software. In particular, HappyTools showed an enhanced throughput demonstrating up to a 10-fold reduction in the total processing time for biopharmaceutical samples. The authors have released the source code and an executable program in an online repository to be employed freely for research purposes.