The 2022 PSI Spring Workshop was held at the European Bioinformatics Institute (EBI) on the Wellcome Trust Genome Campus in Hinxton, United Kingdom, a fitting location for the 20 th anniversary as the inaugural PSI workshop was also hosted at the EBI. This workshop was also the first in-person workshop in three years, since the SARS-CoV-2 pandemic caused the 2020 and 2021 workshops to be held fully online. Previous workshops were held in Cape Town, South Africa in 2019, at the European Molecular Biology Laboratory (EMBL) in Heidelberg, Germany in 2018, and at the National Center for Protein Sciences, Beijing (Phoenix Center) in Beijing, China in 2017. The 2020 workshop was originally organized for the University of California San Diego, California, USA, but was forced fully online due to the pandemic.