Where 𝜎 is the standard deviation, n is the total number of samples, X is an individual sample and 𝜇 is the mean. Given the lack of opportunity to produce replicate samples over the course of this invesigation, the authors are confident that every opportunity was taken to ensure accurate sample preparation and data collection. In pre-candidate selection experimental methodology at GSK, it is commonly accepted to perform high throughput studies without replicates when access to drug substance is limited. Rather, the experimental approach may typically sacrifice statistical significance in favor of generating volumes of data over a larger span of experimental design.