To obtain a soluble peptide for the TM region of UL49.5, the TMC.HSV fragment encompassing TM and C regions was synthetized (Table ). The TMC.HSV peptide showed improved solubility but was not soluble in 100 mM DPC (the solution used to measure NMR spectra). To further improve the solubility of the TMC.HSV peptide three additional lysine residues were added at the N-terminus of the peptide resulting in TMC.HSV(KKK) (Table ) .