Having demonstrated the concept of 'capture-and-release' within a full AmpliFold format, our attention turned towards establishing a model example where the limits of signal amplification could be explored. Here, the receptor density of printed PSA was modulated to achieve a regime where captured sandwich immunocomplexes were uniformly distributed across a large capture area. Whilst poor capture affinity typically detriments the sensitivity of traditional LFAs, we hypothesise that such conditions represent an opportunity where enhanced analyte recovery, over a large capture area, can be leveraged to afford maximal signal amplification, when using a 'capture-and-release' approach.