Anderson pointed out that symmetry breaking was formulated from quantitative to qualitative differentiation in his science paper "More is Different", which underlined the universality of symmetry breaking in complex condensed phases or aggregates. Although symmetry breaking can elegantly explain some common physical property changes at different states, its application in unveiling the optical changes of luminescent materials at different state are scarcely deeply explored except chirality. Therefore, aggregation-induced symmetry breaking (AISB) may have the potential to account for the variations between single molecular state and aggregate state. To get an insight into AISB, a series of closed-form rhodamine-based AIE luminogens (AIEgens) were introduced into this work. Traditional rhodamine-based compounds, with a planar π-conjugated xanthene core, are favored with high absorption coefficient, high fluorescence quantum yield, water solubility and biocompatibility. In most cases, turn-on behavior of rhodamine-based compounds from "closed" to "open" form is widely used, working as fluorescent probes and biosensors. However, both closed and open forms were non-emissive in the solid state, thus solid-state fluorescence would expand the potential of rhodamines. A great endeavor has been put to make the rhodamine-based dyes AIE-active by structural modification. The most frequently used method is the introduction of rotors to the backbone based on RIM working mechanism, such as attaching tetraphenylethene moiety/ planar polycyclic aromatic rings by covalent bonding to the periphery of rhodamine backbones (Scheme 2a). Although these modifications are effective, they usually suffer from complicated molecular design, rigorous synthetic conditions, and tedious organic synthesis. Different from the previous work, we creatively modified the backbone structure by simple synthesis, successfully generating AIE-active rhodamine-based compounds with cross-shaped six-membered structure, as shown in Scheme 2b. Through systematic study, we found that their AIE properties could be understood from a new viewpoint of AISB other than the conventional RIM. Food spoilage detection of seafood was also realized, showing their promising prospect for portable prototypes. The target compounds so-named BISX, ISX, MISX and MTSX were readily achieved via a facile synthetic route from their corresponding imidazole or triazole derivatives using the C-N and C-C coupling cyclization reaction with rhodamine B chloride, according to our previous work. Details of the experimental procedures are provided in the Support Information (Scheme S1). DFT calculations were carried out to investigate the reaction mechanism of MISX (Figure ). All compounds were fully characterized by 1 H NMR and C NMR spectroscopies, high-resolution mass spectrometry, and the obtained singlecrystal structures, respectively (Figure -S13, Table ).