Overall, it is concluded that A3 combination showed better enzymatic activity results in all three districts (Faisalabad, Gujranwala, and Sheikhupura) soil at a pH value of 6.5. So, based on results, it was observed that 6.50-6.67 is the optimum pH for enzymatic action of urease, while urease showed the minimum urea inhibition at a pH value of 3.5. However, the effect of pH on urease activity could be clarified in terms of the changes in the state of ionization of the enzyme.