For the refinement of GSN driven by SAXS-restrained MD simulation, the previous structure was modified. Since the experimental SAXS measurements were collected on a full-length GSN fused to a N-terminal His6-tag, we modelled an additional 23 residues, corresponding to the sequence "MGSSHHHHHHSSGLVPRGSHMAS", resulting in a 778-residue protein that was prepared as described previously. We ran 2x 1µs metainference 61 multi-replica simulations (10 replicas, 100ns each), one with and one without the solvation layer correction enabled. The representative SAXS intensities selected as restraints range between the 𝑞 values of 0.01 Å -1 and 0.25 Å -1 with a stride of 0.015 Å -1 . The analysis was performed on the last 50 ns of each trajectory.