Biocatalysis is a promising field that offers sustainable and environmentally friendly solutions for industries increasingly driven by the remarkable capabilities of enzymes. Thanks to millions of years of evolution, enzymes are fine-tuned to carry out specific chemical reactions with high efficiency. This makes them attractive alternatives to traditional catalysis, which often relies on harsh conditions and toxic chemicals . Thus these biocatalysts find application across various industries, including pharmaceuticals, food production, and sustainability efforts aimed at reducing waste and energy consumption . Since natural enzymes often exhibit suboptimal performance in non-native environments, enzyme engineering is usually required to unlock their full potential . In addition to commonly used experimental approaches such as directed evolution, scientists can also expedite the process and reduce associated development costs by incorporating computational methods . These methods help navigate the vast sequence space, as it is estimated that only a fraction of all possible sequences fold into functional protein structures . Most natural proteins have marginal stability , thus posing a significant risk for any manipulations with their sequences. Many computational methods aiming to reduce the search space rely on homologous sequences . These sequences of different but related proteins stemming from a common ancestor contain rich evolutionary information . Homologous protein sequences can be employed to identify conserved and functionally important regions, suggest beneficial mutations, and create phylogenetic trees to reconstruct ancestral sequences . Ancestral sequence reconstruction has proven to be a promising strategy for enhancing protein stability .