The thermodynamic solubility of analogs was determined via an adapted protocol from literature. Compounds were dissolved at a concentration of 0.5 mg/mL in DCM (minimum total volume 1.5 mL). 250 µL of this stock were transferred in triplicate to three separate HPLC vials equipped with stirbar, designated as experimental vials. A separate 200 µL was transferred to an additional HPLC vial for use as the standard. The DCM was then evaporated via gentle stirring on hotplate at 40°C for several hours. Vials marked as experimental were then diluted with 500 µL phosphate-buffered solution (pH 7.4), and the vial marked as standard was diluted with 500 µL DMSO. The vials were then stirred at room temperature for at least 24 hours. The stirbars were then removed and the vials were allowed to sit at room temperature for an additional 24 hours. The vials were then centrifuged to pellet undissolved compound and the supernatants were then injected on HPLC. Determination of thermodynamic solubility was then ascertained by comparing the integrated AUC of the standard vial compound peak (known concentration of 200 µg/mL) to the integration of the experimental vial compound peaks (AUCexp./AUCstd= Conc.exp/Concstd where Conc.exp= measured thermodynamic solubility).