For the hydrogen bonding reference datasets, RS24 introduces organic compounds containing N-H bonds. These mainly include primary/secondary (1 ○ /2 ○ ) amines. RS25 introduces organic compounds with O-H bonds, including alcohols and carboxylic acids. The remaining reference datasets once again use various combinations of the functional groups from the non-hydrogen bonding and hydrogen bonding sets to fit cross-interactions. Additional functional groups such as amides or oximes can also be found in the later reference datasets (RS26-RS34).