The current approach to VTNA experimentation involves performing a "standard" reaction, under synthetically relevant conditions, followed by "different excess" experiments, where only the initial concentration of each reaction species is altered. This experiment design we define as "sequential VTNA", as the orders are obtained for one reaction species at a time by identifying the order that makes the standard and relevant different excess profiles overlay. Pleasingly, we found that Auto-VTNA could identify the order values giving optimal overlay in all cases using sequential VTNA. A monotonic polynomial degree of 5 was sufficient in most instances to avoid inaccurate order values due to underfitting. However, for concentration profiles exhibiting significant curvature, a 7 th degree polynomial was necessary to ensure convergence to the optimal overlay order value (see ESI, Section S3.3)