The downstream effects of • NO-scavenging by the different compounds were further evaluated via wound healing assay. While DETA-NO enhanced the rapid closure of the gap, its addition to the cells in combination with each compound caused inhibition of the cell migration with different rates. This was dependent on the type of added compound and its concentration. ). Moreover, at 8 μM, all compounds except C-PTIO also inhibited cell migration. One of these effects may relate to the interactions of these compounds with one or more of the pathways involved in cell migration (Supplementary fig. ). For that, and as the first step to elucidate that, the cellular uptake of the different hemin compounds was measured via quantification of total iron after incubation with cells in the presence and absence of DETA-NO.