This study also aimed to determine the effectiveness of a novel, cost-effective spectroscopic method of detection for the Fenton reaction. Significant research has been done on the direct effect of radiation on DNA, yet there is limited research on the reactions between hydroxyl radicals and DNA that make up a substantial portion of mutations. This study incorporates a new method of determining the environment in which hydroxyl radicals are readily produced through the spectroscopy of ferric thiocyanate. Many other studies take advantage of NMR spectroscopy to directly measure the concentrations of radicals produced which can be costly and limited. One of the biggest disadvantages of the Fenton reaction is high cost [3] and using UV-Vis spectroscopy will allow for the study of the production of hydroxyl radicals to be more widespread and cost-effective. This is pivotal for future study in how these lead to the development of cancer and determine methods of regulating these radicals for patients who undergo radiotherapy.