Encouraged by the success of the 800-mer syntheses, considering that the CBP method can potentially pick up oligos with unlimited length from crude mixture with unlimited complexity, we decided to synthesize the 1,728-mer Φ29 DNA polymerase gene. Because glass beads had close to zero errors, and had a higher percentage of correct sequences than glass wool (96% vs 85%) for the 800-mer syntheses, glass beads were chosen for the synthesis. The synthesis, deprotection and cleavage, and CBP purification procedures as well as PCR amplification, cloning, and Sanger sequencing were the same as described for glass wool. The scale of the synthesis was 33.28 nmol, which corresponds to 130 mg glass beads. The quantity of oligo obtained was 2.83 µg (5.28 pmol) for the synthesis using 130 mg glass beads (41 pmol/g, Entry 9, Table ). The overall yield for the entire 1,728-mer synthesis and purification was 0.016% (Entry 10), which is similar to that of 800-mer synthesis using glass beads as support.