The simulations with OPC3-pol water were prepared similarly as the ff19SB+OL3 simulations with OPC water model, with a few exceptions. The water box of OPC3-pol water is not equilibrated, and thus the box buffer was increased to 12Å. The increased buffer size lead to about the same number of water molecules that was present with the OPC simulations. The solvent box dimensions were then added using tleap to match the initial dimensions of the equilibrated OPC box from the previous simulations. To equilibrate this system more gently, the first equilibration simulation length was increased from 400ps to 1ns. The production run parameters were similar to the other Amber simulations.