EH@Ag2S +US, (5) PBS -US, (6) EH -US, (7) Ag2S -US, and (8) EH@Ag2S -US by live/dead staining (calcein AM/PI) experiments. It had been found that the introduction of EH@Ag2S combined with US stimulation (1.0 W cm -2 , 1.0 MHz, 50% duty free) significantly induced MC38 cells death, and treatment effect was consistent with the ROS production (Fig. ). These results indicated that EH@Ag2S co-assembly could generate a large number of ROS, and the ROS generation could be further enhanced compared with Ag2S at the cellular level, which might be relevant to the targeting effect of EH that enhanced the cell uptake of EH@Ag2S.