by centrifugation at 2000 rpm for 10 minutes. The plasma was warmed to 37 °C, and the compound solution was added to achieve final concentrations of 50 nM and 1 µM, respectively. A control was prepared in 4% BSA (w/V) in water containing 1 µM of the respective compound. Each approach was applied in triplicate. All samples were incubated at 37°C and shaken at 400 rpm over 24 h. Sample collection was conducted 0, 30, 60, 120, 240, 360, and 1440 minutes. The samples were immediately precipitated with ice-cold acetonitrile, which contained the internal standard. Following vortexing and shaking for 30 minutes at 1000 rpm, the samples were centrifugedated for 10 minutes at a speed of 14,000 rpm. The supernatant was evaporated under a gentle stream of nitrogen at a temperature of 37 °C and the residues were covered and stored in a refrigerator at 4 °C. Following the final sampling period, all residues were reconstituted in 70/20/10 water/dimethyl sulfoxide/formic acid (v/v, containing 10 % formic acid) and analyzed.