So far, the utility of the 'capture-and-release' strategy has been demonstrated to highlight the improvements in sensitivity that can be obtained when low affinity surface capture limits assay performance. This same issue also affects the surface anchoring of larger, higher signal nanoparticles in LFAs where reduced diffusivity and sterically hindered collisions between immunoassay components at the solid phase reduce sensitivity. Here, we assess the performance of large nanoparticles, and particularly their deployment as detection labels in the AmpliFold architecture.