Coulter-counter or resistive-pulse sensing is widely used to characterize particle size, charge, shape, and concentration. Resistive-pulse sensing was introduced in 1951 as an automated technique to count and size red blood cells, and is still commonly used today. In resistive-pulse sensing, a cell or particle is detected as it passes through an opening separating two ionically conductive solutions by partially blocking the electrical current established between the electrodes placed on each side of the opening. In this work, the orifice of a laserpulled glass nanopipette is used to resistively sense the translocations of liposomes as they enter and leave the nanopipette. Recent biological applications of resistive-pulse sensing include the characterization of exosomes originating from the MDA-MB-231 breast cancer cell line, delivery of liposomes containing redox active species to electrode surfaces, deformation measurements of multilamellar liposomes and pseudo-type human immunodeficiency virus (HIV-1), and characterization of DNA. The characterization of biologically relevant particles via resistive-pulse sensing generally has limited sizing precision when translocating the particle one time through an opening separating two electrolyte solutions. While single passing particles enable high throughput measurements, there is ~15% error in the measured size based on a single translocation. This error is due to variations in the position of the particle as it passes through the sensing zone. Particle translocation near the edge of nanopipette results in slightly larger resistive pulse blockage currents compared to particle translocation through the center of the nanopipette. To overcome this source of error in the resistive pulse measurements, multipassing particles through the nanopipette orifice many times enables extremely high precision measurements of the particle size. For example, multipassing a single 250 nm radius polystyrene (PS) particle as few as 10 times reduces the standard error (SE) of the measurement to less than 1 nm. The application of multipass resistive-pulse sensing to characterize biologically relevant particles (e.g., liposomes and EVs) would enable precise sub-nanometer size characterization on an individual particle basis. Combining fluorescence imaging with multipass resistive-pulse sensing enables simultaneous sub-nanometer sizing precision and the detection of fluorescent markers on the surface of biologically relevant particles. Fluorescent markers are commonly used to detect specific proteins on the surface of a biological particle. The combination of these two methods provides a powerful means to simultaneously characterize the presence of surface proteins and the size of biological particles.