The reactors were inoculated with different sources: R1 with AS, R2 with AD, and R3 with CM. The reactor setup, inoculation, and flooding procedures followed the protocol previously detailed in . For the initial 20 days after inoculation, reactors were only flooded weekly without additional feeding of nutrient media. After this period, each reactor received a weekly feed of 200 mL nutrient media, with an equivalent volume being removed to maintain a steady liquid level in the sump.