The major limitation of POC devices developed for STEC from agri-food samples is usually the very low concentration of target bacteria among the existing other species in the same sample It is worth mentioning that these low bacterial counts can cause life-threatening infections. Main approach in order to increase the concentration of the target DNA is including the amplification step. For this purpose, PCR is the most common technique, however, it requires a high precision thermal cycler for temperature control, and long sample preparation, and is prone to non-specific amplifications . These working conditions of PCR limits the application for POC application. The alternative approach which has gained attention in the recent years is isothermal amplification such as loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), helicase dependent amplification (HAD), or rolling circle amplification (RCA) . The details of each technique have been previously described in the reviews by and . In brief, the isothermal amplification techniques include enzymatic DNA denaturation, which allow to eliminate the high temperature application for denaturation step of PCR. This approach provides an opportunity to use a simple device which can keep the temperature constant for primer annealing and extension.