Overall, we tested the C-MST assay on 10 reference compounds whose tubulin binding structures were resolved using X-ray crystallography and available in the PDB, and over 30 additional compounds, whose interactions with tubulins were characterized using alternative methods in this study or elsewhere. Based on these tests, the assay has shown the accuracy in the range of 91-97%, the sensitivity in the range of 81-95%, and the specificity close to 100% (see Methods).