Brightfield imaging with a 20x magnification lens (Nikon Plan Fluor) and a depth of field 2.5 -4.5 µm was used along with a high speed camera (Edgertronic SC2+) of maximum framerate of 31,000 fps and minimum exposure time of 10 µs. The resulting resolution is 0.67 µm/pixel, and the maximum particle displacement per frame is 17 µm/frame at 20 µL/min as compared to a field of view that spans 170 x 68 µm at 31,000 fps. A custom built MATLAB code that incorporates topological constraints and symmetries in the flow field was used for accurate tracking at these extreme flow conditions. Higher flow rates could not be used due to the limitations on the field of view and frame rate inherent to the camera.