However, the combination of nitrification and urease inhibitor could significantly reduce the N loss and improve the environment. The hydrolysis rate of urea increased in control as compared to inhibitors treated samples with an increase in incubation time, which reported that A3 combination presented the better reduction of urea hydrolysis as compared to control (urea alone). However, the minimum urea hydrolysis of about 1.58 IU/g soil was observed for Faisalabad cultivated soil after 10 th day of observation, while control (urea alone) value was noted 18.24 IU/g soil, which showed that A3 combination presented much exceptional inhibition results than control. Similarly, the minimum urea hydrolysis of about 2.54 and 2.13 IU/g soil was observed after 4 th and 6 th day of observation, with the rate of 0.50% for Gujranwala and Sheikhupura, respectively. While the control showed 19.13, and 27.65 IU/g soil inhibition for the same districts, which indicated that A3 combination presented much more prime urea hydrolysis results as compared to control for all three districts. Generally, when soil was not treated with ammonium thiosulfate (ATS), nitrate rapidly increased throughout the two weeks of incubation period; however, using ATS delayed the initial nitrification rate and significantly reduced NO -3 formation. It was reported that ATS has the ability to delay nitrate formation .