This study describes the preparation of a bioink for the inkjet printing of biosensors for the detection of hepatitis B surface antigen (HBsAg). The ink consisted of single-walled carbon nanotubes (SWCNTs) conjugated with anti-HBsAg antibodies (SWCNT-Ab). The ink was inkjet printed by an office printer as rectangular channels to form a conductive film for the detection of HBsAg. Multiple layers of ink were printed to achieve sufficient electrical conductivity. The demonstrative biosensors showed a linear response range from 1-10 mM HBsAg, with a detection limit at 0.5 mM. The study concludes that the inkjet printing of biosensors using SWCNT-Ab bioink is promising for producing large scale and lowcost sensors with high sensitivity for HBsAg detection. The results suggest that this method could have potential applications in resourcelimited settings for the early detection and accurate diagnosis of HBV.