This work demonstrated that it is possible to expand the design of multicomponent supramolecular hydrogels in minimalistic tripeptides, by including sequences with the Phe-Phe motif to promote selforganisation that differ not only in the third amino acidic residue, but also in overall stereoconfiguration. In particular, binary systems featuring His-Phe-Phe and Asp-Phe-Phe, either with the same or with the opposite stereoconfiguration, were successfully co-assembled into nanofibrillar hydrogels. The viscoelastic behaviour of these systems showed that adding Dff to Hff resulted in dramatically faster gelation kinetics, albeit the elastic modulus and the hydrogel resistance against applied stress were similar. We inferred that the negative charge of Asp sidechain played a favourable role in the selforganisation, likely establishing electrostatic interactions with the cationic N-terminal ammonium groups. Conversely, changing also stereoconfiguration of the components in the hydrogels obtained from Dff and hFF significantly slowed down gelation, suggesting that self-organisation was more difficult in this case. The hydrogels displayed lower elastic modulus that could suggested more defects in the supramolecular packing. The high heterogeneity of Dff-hFF samples was found also in terms of the nanofibrils widths' wider distribution relative to Dff-Hff that appeared more homogeneous. Nano-IR data confirmed lower variance in the latter sample, and it provided useful insights into the packing modes, suggesting co-assembly into amyloid-like fibers in all the two-component systems. Overall, we find this technique very useful to characterise multicomponent and supramolecular hydrogels and to reveal fine structural details that can discriminate between self-sorting and co-assembling gelators.