Fr. 1 8.39 mg (7.1%), Fr. 2 90.49 mg (76.0%), Fr. 3 6.43 mg (5.4%), Fr. 4 12.06 mg (10.1%), Fr. 5 1.71 mg (1.4%), Fr. 6 < 1mg (<1%). The curcumin knock-out materials obtained from both CDS were then compared with previously reported curcuminoid-enriched turmeric extracts. Using authentic reference compounds for comparison, Fr. 1 showed the presence of 4, Fr. 2 was identified as 1, and Frs. 3 and 4 represent minor amounts of 1 eluting at the tail-end of the chromatographic peak of 1. Observed NMR signal shifts are likely due to differences in residual water, concentration, and interaction/complexation with metal ions. However, the congeners 2 and 3, which are more highly retained in this SS, could not be detected at all, indicating the absence of a natural mixture of natural curcuminoids in the CDS. The polar fractions 5 and 6 showed the typical signals of saccharides (3.0 -5.2 ppm) (Figure ). CCS-based curcumin knock-out methodology is orthogonal to NMR, widens the dynamic range of subsequent analysis (see also Figure ), and, by virtue of its liquid-liquid partition mechanism and 100% recovery, can help confirm (or refute) the phytochemical integrity of a metabolome.