Water-dispersible g-Odots were prepared by encapsulating these emitters and the mCP host in an amphiphilic DSPE-PEG2k polymer. The MeODPA-DiKTa g-Odots showed a deeper red emission at lPL of 663 nm compared to the PhDPA-DiKTa g-Odots (lPL = 618 nm). Both families of g-Odots maintained their TADF properties, with td of 203.9 µs for PhDPA-DiKTa g-Odots and 131.6 µs for MeODPA-DiKTa g-Odots. These g-Odots were successfully used as biological imaging probes of HeLa cells, demonstrating the feasibility of single-photon excited microscopy measurements. This work highlights the potential of MR-TADF emitters in bioimaging applications, combining high color purity and TADF properties for improved imaging performance.