We assess the Jaccard similarity between all pairs of peptides in the local neighborhood by considering the sets of overlapping k-mers that describe the sequence, where a k-mer of a peptide is a subsequence of length k, and the spectrum of k-mers has previously been demonstrated to contain significant information about AMP properties . We consider k-mers of lengths two and three in this analysis. 0 ≤ J(A, B) ≤ 1 by design, and a Jaccard similarity of zero implies that two sequences share no subsequences of length k whereas a Jaccard similarity of one implies that two sequences are composed of identical subsequences.