Figure shows representative distributions exhibiting a high degree of overlap between systems. Further, the only structural property distribution for which both defect systems simultaneously exhibited a slight, but noticeable offset from the wild-type distribution was in the case of the major groove depth. However, the observed difference of the order of less than 0.1 Å can still be considered negligible. Furthermore, because regions 1 and 2 both reside in the minor groove, there is no obvious correlation between the location of region 1 and 2 and a possible impact on major groove. Figure shows the slight offset for both defect systems in the major groove property.