all the systems, we find a co-existence of two humps and the conversion of the first hump to a sharp peak at higher reline concentration. The stability of these two peaks can be expressed in terms of change in free energy and the position of their respective minima. This point out the propensity of a system to move from the highly structured region to the locally stable unstructured region and viceversa. The change in free energy (∆𝐺) is calculated as follows,