A common characteristic of intI1 is its occurrence alongside sulphonamide resistance (M. ). As a result, positive correlations are usually observed between abundances of intI1 and sul1 in wastewater . Results in this study showed agreement with this, demonstrating strong positive correlations between absolute and relative wastewater loads of intI1 and sul1 (r = 0.90, p = < 0.05 absolute loads, r = 0.90, p = <0.05 relative loads). Correlations of intI1 with other genes investigated were not observed (table and). WQIs for selected samples over the studied period were investigated (figure ). In both Bath and Keynsham, the average monthly values of certain WQIs parameters showed statistically significant positive correlations with each other (table ). When investigating relationships between monthly AA concentrations and physiochemical parameters, Bath showed limited statistically significant correlations (table ). Regarding Keynsham, negative correlations were observed for the loads of certain AAs with certain physiochemical parameters, including BOD, TOC, and dissolved organic carbon (table ). In other studies, Zieliński et al. have reported, positive correlations between AA including clarithromycin, ofloxacin, and sulfamethoxazole with physicochemical properties such as BOD, COD, and TOC .