Sequences for the enterovirus genus were identified using The National Center for Biotechnology Information's (NCBIs) "Taxonomy Browser" database. Archetypal representative strains, exclusive to human reference sequences in the enterovirus genus, were selected and aligned using MUltiple Sequence Comparison by Log-Expectation (MUSCLE), which is found in open source Molecular Evolutionary Genetics Analysis (MEGA) software. The longest uniformly conserved nucleotide base sequence amongst reference isolates returned was 23 base pairs long -5' ACU UUG GGU GUC CGU GUU UC 3', residing in the 5' non-coding region of the viral genome. NCBIs Basic Local Alignment Search Tool (BLAST) highlighted this sequence was exclusive to enteroviral genus species and had 100 % identity matches with 8291/8648 (96%) sequences. These 23 bases were selected as a target from which a bespoke aptamer sequence was designed.