From this MIPE4 library, the most active 32 molecules were selected based on their IC50 and curve response class (CRC) for further matrix combinations. They were run in "all versus all" combinations, totaling 496 unique pairwise combinations, as 10x10 matrices/blocks where two compounds were applied in nine 1:2 serial dilutions. The concentration range for each compound was selected individually to arrange their IC50 roughly in the middle of that range. Each block had a DMSO control (IC0), as well as Bortezomib, a well-known cancer drug, as the positive control (IC100). Each block was tested in duplicate.