To assess the achieved improvements in the optimized cholesterol model, we computed a range of observables for the original and optimized cholesterol models. To show that our approach does not alter the original model in any detectable fashion, we extensively compared the Solvent Accessible Surface Area (SASA) and the equilibrium and RMSD values of pairwise bead distances of the single isolated cholesterols. The SASA was computed using gmx sasa and a probe sphere of radius 1.85 Å, while the bead distances were computed using a PLUMED 2.7 script.