In turn the architecture of the brushes generated and their solution morphology regulated the adsorption of BMP2. Dense and thick PSPMA brushes led to significant levels of adsorption of BMP2, whilst this was reduced on thinner brushes, regardless of their density. In contrast BMP4 did not adsorb as significantly, perhaps reflecting differences in the charge distribution and associated electrostatic potential at the surface of the corresponding proteins. QCM-D data also indicated the surface adsorption of BMP2 to thick dense brushes, with some evidence of infiltration within thinner coatings, perhaps as a result of increased polydispersity at early polymerisation time points. Overall, these results indicate a predominantly secondary surface adsorption to polymer brushes, in particular with high densities and thicknesses. This is in contrast with the adsorption of nucleic acid molecules adsorbing deeply into cationic brushes .