Having established the amplification system, we then sought to apply this chemistry to a synthetic material. For this, we fabricated hydrogel structures using N,N-dimethylacrylamide (DMA) with N,N'-cystamine bis(acrylamide) (BAC) crosslinks (3.5 wt%) by free radical polymerization . We anticipated that upon SH-analyte-sensing, compound 1 releases TPPTS inside the redox active material matrix. Subsequent amplification reactions reduce internal disulfide crosslinks, thereby physically transforming the hydrogel from gel to sol, making the process visible to the naked eye (Figure ). Since analyte detection occurs through the amplification system, only sub-stoichiometric amounts of SH-analyte are needed to trigger material dissolution. Consequently, we studied the material at hand by exposing it to various biologically relevant SH-analytes and evaluated their sensitivity and application for naked eye analyte detection.