The next step was to verify our hypothesis regarding the anion transport ac7vity of the synthesised compounds T, TT, TA, and TB. For this, we have chosen the commonly used lucigenin assay because it is a convenient method that can be used at different pH levels. As azine metathesis was found to be most efficient in the presence of an acid A pulse of NaCl was added to the exterior of the liposomes to create a Cl -gradient of 25 mM and the transport process was monitored by fluorescence spectroscopy. When Cl -is transported into the liposomes, it quenches the fluorescence of lucigenin. The NaNO3 solu7on is used to allow the displacement of charge upon transport of Cl -into the liposomes to be balanced by the transport of NO3 -out of the liposomes via an an7port mechanism.