The findings suggest that the building protocol is reasonable, with MC3 LNP serving as a control due to its known formation of an inverse hexagonal phase with polyA RNA at pH 6-4.5 . For Lipid 10 and 2 there is no experimental data on their organization at low pH, however, we do observe Lipid 2 adapting a completely different organization rather quickly. This shows, that even though we pre-built with an inverse hexagonal phase, other organizations can easily be observed and that the LNP, at least in this case, is not stuck in the inverse hexagonal phase.