Several methodological limitations should be considered when interpreting these results. The quantum chemical calculations underlying our kinetic predictions carry inherent uncertainties from the use of PCM solvation models, DFT-level electronic structure methods, and finite conformational sampling. Recent benchmarking studies in related reaction networks suggest these approximations introduce uncertainties of 3-6 kcal/mol in computed barrier heights and reaction energies. While these uncertainties affect quantitative comparisons between specific reaction pathways, the qualitative conclusions about reaction preferences remain robust given the larger energy differences observed between competing channels. A more fundamental limitation arises from restricting reaction exploration to b2f2 mechanisms within one step of the main cycle. More complex reaction sequences involving three-bond changes or multiple steps could potentially enable parallel cycles that bypass the identified kinetic bottlenecks. The water-catalyzed mechanisms investigated here also represent a minimal model of solution-phase catalysis, as certain reactions might proceed through mechanisms involving multiple water molecules or alternative catalytic species. The systematic computational approach developed in this work enables broader investigations of prebiotic carbon-fixation chemistry. This methodology can be directly applied to analyze other anaerobic carbon fixation cycles proposed to operate under early Earth conditions, to interrogate their kinetic viability and catalytic requirements. The reaction network exploration strategy demonstrated here can be accelerated through machine learning assistance in TS searches and reaction pathway prediction, enabling more exhaustive mapping of the chemical space around carbon fixation cycles. Such enhanced sampling could support discovery of de novo carbon fixation cycles optimized for specific criteria such as kinetic efficiency or minimal catalytic requirements. The resulting fixation cycles could provide new hypotheses for prebiotic chemistry while suggesting efficient chemical pathways for carbon dioxide reduction and sequestration.