After measuring urease inhibitor potential, different urease kinetic parameters including temperature, pH, and incubation time were calculated. The colorimetric procedure was used to evaluate the effect of temperature by changing the temperature of the incubation period as 10, 20, 30, 40, and 50 o C for soil samples. The effect of pH on urease activity in soil samples was evaluated by changing pH as 3.50, 4.50, 5.50, 6.50, and 7.50 . Similarly, the effect of incubation time was observed by changing incubation time from 5, 10, 15, 20, and 30 min by adopting the method of Khan et al. with little modifications.