Specifically, the eluate collected from a PFKDOPA-coated beads column yielded negligible absorbance at 380 nm (Figure , blue), indicating complete hydrolysis. In contrast, both nitrocefin eluent (prior the insertion into the column), and the eluate collected after passage of nitrocefin in a column containing bare silica beads (not displaying PFKDOPA), produced spectra showing the significant nitrocefin signals at 380 nm, indicating no hydrolysis. Figure , inset, demonstrates the recyclability of the coated beads; essentially, ~90% degradation was achieved even after five consecutive cycles, indicating that the PFKDOPA amyloid fibrils attached to the beads remained catalytically active through multiple β-lactam hydrolysis cycles.