182 out of 412 AMS hits and 17 of the 28 confirmed Enamine hits matched computational AlphaScreen frequent hitters filters, suggesting some fraction of our hits would be removed by a confirmatory screen. Nevertheless, the high initial hit rates, potency, and chemical diversity of our prospective screens is quite encouraging. The next steps after validating our AMS and Enamine hits would include screening and optimizing for specificity, toxicity, metabolic stability, and bacterial growth inhibition.