Raman Spectroscopy is a non-invasive and versatile technique often employed as a technique to overcome this challenge in sensing applications . Raman scattering is inherently weak due to the low probability of the Raman process , but adsorbing analytes on the surface of certain metal nanoparticles can increase the Raman signal by a factor of 10 2 -10 14 through surface-enhanced Raman scattering (SERS) caused by the excitation of localized surface plasmon resonances of nanoparticle coinage metal resonators . SERS can detect analytes at very low concentrations, which makes it very useful in the study of complex molecules like proteins by reducing the requirement for sample concentrations . When integrated with scanning probe microscopy (SPM), SERS is modified into Tip-enhanced Raman spectroscopy (TERS), capable of probing the configuration of small molecules on gold or silver nano-tips, with singlemolecule resolution .