The isoprenoid synthase superfamily (InterPro No.: IPR008949) was queried against the UniProt database, yielding over 200 thousand matches. These results were refined by taxonomy (P. clavata) to obtain 23 relevant matches. The sequences associated with terpene biosynthesis in P. clavata underwent phylogenetic analysis with the neighborjoining method and were annotated using the Family & Domains databases within UniProt (Fig. ). Besides the short sequences and polyprenyl synthase related to the synthesis of terpene diphosphate precursors, the 11 identified TS genes, featuring conserved motifs typical of type I TSs (Fig. , Supplementary Figure ), were categorized into 8 groups. Subsequently, one representative from each group was synthesized into the bacterial expression vector, pET28a, for activity assessment.