Moreover, the bore diameter of the detector flow cell plays a crucial role and must be dimensioned according to the size of the analyzed particle. However, adjustments of detector flow rate did not improve the accuracy of the size measurements, and after discussions with the manufacturer of the MALLS and DLS detectors used in this study, it became clear that the bore of the installed flow cell of the MALLS detector was simply too narrow and thus not suitable for online DLS measurements of particles with radii > 50 nm. While a flow cell with a wider bore diameter was not available for our MALLS785nm-it was possible to measure the LNP samples on a AF4 system hyphenated with a MALLS detector (λ= 660nm) equipped with a wide bore flow cell and a build