After successful in vitro testing, we scaled and translated mRPZ into an in vivo gene delivery model using Balb/C mice. Instead of eGFP expressing mRNA, utilizing the same synthetic encapsulation process, we encapsulated 12 µg of firefly luciferase mRNA (1920 nt). Firefly luciferase generates bioluminescence in the presence of luciferin, which can be injected into mice to monitor luciferase expression (Figure ). The work performed here demonstrates one of the first applications of mRNA delivery using a MOF platform in vivo.