However, to understand which mechanism is more plausible, let us consider the kinetic aspect. For this, assume a solution of 0.1 M total glycine reaching the isoelectric point. Equation (10) can be used to estimate the concentration of H₃O⁺, assuming no other reactions occur. That is, the first equilibrium will produce equal amounts of H3O + and A -, which is set to x in the following: