To implement UVPD fragmentation on the ZenoTOF 7600 system, the EAD cell was modified, and one of the two EAD filaments was replaced by a mirror (12.7 mm diameter -45° angle) to redirect the UV photon beam through a 12.7 mm diameter UV-visible grade calcium fluoride CaF2 window into the center of the EAD cell. To get the photons from the laser inside the vacuum chamber, a high transmission UV-visible grade calcium fluoride CaF2 window was mounted on the vacuum chamber cover (outside diameter 25.4 mm, 2 mm thickness). The laser used was a 266 nm laser Nd:YaG laser (Teem Photonics S.A, Meylan, France) with a power of approximately 0.5 µJ (4.7 eV per photon) and pulsing at a frequency of 19 kHz. EAD and UVPD fragmentation techniques were operated in two different modes: the flow-through mode or the simultaneous trapping mode. These two modes were first described by Takashi Baba et al. on a Q-TOF instrument. In the simultaneous trapping mode, a potential is applied at the exit of the EAD cell to trap precursor ions within the cell while they are irradiated with UV photons or electrons. In the case of the flow-through mode, the process occurs in two steps. First, precursor ions are trapped by applying a potential at the exit of the EAD cell, accumulating a certain number of ions. Then, in the second step, another potential is applied at the entrance of the EAD cell to trap the ions without allowing new ones to enter, and UV photons or electrons subsequently irradiate them. The experiments used simultaneous trapping mode for both EAD and UVPD fragmentation techniques. Product ion spectra of standards were acquired for each activation technique by LC-MS under the following conditions: i) CID, the collision energy range was from 10 to 100 eV with steps of 10 eV, ii) EAD, the electron kinetic energy range was from 0 to 25eV with steps of 2 eV and, iii) UVPD, the reaction time (irradiation time) was in the range 60 msec.