Our interaction network data also shows an increased number of interactions for Chimera 3 at the N-terminal portion of the WPD-loop (Figure and D) as a result of the G352T and N353T substitutions. Consistent with this, our RMSF calculations determined this part of the WPD-loop to be more rigid in Chimera 3 than in wild-type YopH, but failed to reject the null hypothesis.