After the hydrogenation process, 10 mg of the oil sample was directly used for transesterification and GC analysis following the FAME analysis cited above. However, after the sulphur polymerization process, the polymer containing oil was subjected to ethanol extraction (20 mL) at 50 ºC for 2 hours. The ethanol in the solvent extract was removed by rotary evaporation at 50 °C, and lipid weight was determined gravimetrically. 10 mg of this dried lipid was used for transesterification and GC analysis.