To demonstrate the approach could be used to target specific pockets on a protein, the Fusicoccin pocket on 14-3-3 was then targeted with an extended library of aldehydes (165 in total). Two longer hydrazide peptides were synthesized (Fig. ) extended by one (hDMX361-370 pSer367 Gly-hydrazide) and two (hDMX361-371 pSer367 Gly-hydrazide) residues so as to reach the Fusicoccin pocket. Similar results (in terms of potency) were obtained for each peptide in the dynamic ligation screen; thus for clarity, only data for hDMX361-370 pSer367 Gly-hydrazide are described here (Fig ; screening and validation, including for hDMX361-371 pSer367 Glyhydrazide is shown in the supporting information Fig ). More hits with a hDMX361-374 pSer367 */14-3-3η response greater than the control were identified in this second round.