have already been investigated in detail in a number of computational and experimental studies, including DLPNO-CCSD(T)/LED investigations. The protein-ligand binding energies computed at This analysis allows us to quantify the strength of this interaction considering all cooperative effects within the receptor, revealing a significantly stronger binding energy to the protein for nicotine compared to imidacloprid. The interaction between the ligands and the S142/W143/T144 moiety accounts for 79% of this difference.