To begin an experiment, a paper Wilhelmy plate was first soaked in the subphase for 30 minutes. An ethanol-cleaned, fully dried black glass plate was placed in the trough to prevent unwanted radiation scatter from the BAM laser. The trough was then filled with ca. 52 mL of subphase. The plate was then transferred to the balance hook and lowered to the first point of contact with the subphase in the trough. At this point, the balance was set to zero. A micro-syringe was then used to spread a monolayer on the subphase using ca. 20 μL dropwise. This was accomplished by depressing the plunger just enough to create a droplet and then touching this droplet to the subphase without contaminating the syringe. Droplets were deposited in different locations to spread the monolayer effectively. After sufficient monolayer was spread, the chloroform was allowed to evaporate for 20 minutes. The BAM head was positioned over the trough and initial images were collected. Compression with a single barrier was then carried out at a rate of 10 mm/min and images were frequently taken to observe morphology changes in the monolayer, with the surface pressure balance readings manually recorded with each image, up to ca. 30 mN/m. Images were then analyzed and features unique to LPS or lipase subphases that did not occur for NaCl only control experiments are presented in this report.