Chemical shift perturbation measurements and relaxation experiments (T1, T2, heteronuclear ( 1 H, 15 N) NOEs) were performed, in the absence and presence of AM-7209, for WT and I19G (~1 mM and ~480 µM protein concentration respectively and complexes in molar ratio 1.2:1 ligand:protein). NMRPipe was used to process all the 2D and 3D spectra and subsequently analyzed using NMRFAM-Sparky and POKY. The same samples were used for backbone