Another new approach to improve materials and enzyme immobilization for the construction of an impedimetric glucose biosensor is proposed. The approach involved silanization of titanium dioxide nanoparticles with (3-aminopropyl)triethoxysilane (APTES) for the preparation of cross-linked material nanoparticles with carboxylic graphene. The modified electrode showed an increase in electron shuttle and was used to develop the impedimetric glucose biosensor. The biosensor demonstrated a wide operating linear range of 50 µmol to 1000 µmol and a low limit of detection of 24 µmol. The results suggest that the proposed method can be useful in the development of low-cost and easy-to-use detectors for medical use.