In addition to the time-dependent salt equilibration, the model analysis further reveal that the overall vesicle size is slightly reduced while the size polydispersity is increased, immediately after salt addition. The vesicle size then relaxes back towards that of the reference in salt free buffer during the course of the equilibration, and the effect appears to be slightly more pronounced for LL-37 at 1:100 and indolicidin at 1:50 PL ratios, which displayed the slowest transport. This can perhaps be attributed to the remaining osmotic pressure before the salt concentration is fully equilibrated, as the effect is transient and almost non-existent in the case of aurein which display the most rapid equilibration. It should be noted, however, that transient shape fluctuations potentially resulting from the initial osmotic pressure, or even the turbulent mixing, can have a very similar effect on the scattering curves as that of polydispersity, which makes the two difficult to distinguish. Furthermore, a very small reduction in the lipid volumes is observed, with ∼ 1 % for the lipid tails and ∼ 5 % for the lipid heads. While this can perhaps be attributed to charge screening and solvent effects, these values are very close to, or within, the experimental uncertainty and no strong claims can be made.