For fluorescence spectroscopy 198 µl sample solution was placed in a 96 well plate. For analyte sensing, 2 µl of the analyte was added to the sample solution. The well plate was placed on an IX73 microscope (Olympus) and fluorescence spectra were acquired by a Shamrock193i spectrometer (Andor Technology Ltd.), connected to the microscope. Sample excitation was performed using a gem 561 nm laser (Laser Quantum) at 100 mW excitation power.