Until now, we have discussed the variation across long timescale MD trajectories and emphasised that ensemble simulations are necessary for UQ irrespective of the duration of simulation. However, as already discussed in section 1, several accelerated sampling protocols (including the splitting protocol employed in this study) that are based on performing "ensembles" are also expected to exhibit similar variation and would require performing ensembles for UQ. We have already shown this for replica exchange methods in some of our previous works. Nevertheless, this aspect has not been addressed adequately in the literature for other accelerated sampling protocols as the reported errors for such methods are all derived from the data generated from a single execution of the protocol, but never from ensembles comprising multiple instances. One reason of this shortcoming might be the computational cost associated with all these methods. We hope to return with a subsequent study where we will discuss this issue systematically.