Structures of higher spatial complexity (e.g. the 12-fold and 8-fold bundles) had low folding yields due to splitting defects and showed significant internal disorganization even in fully compacted structures. Internal disorganization may also be responsible for the observed flexibility in simpler bundles, as tsDNA is reportedly stiffer than dsDNA and there are no DNA backbone interruptions in dsDNA regions that could produce high levels of local flexibility.