A stock solution of polymer, MilliQ water, and solvent (if a third pump was connected) were prepared and positioned so that the tubing was immersed in the solution while still being covered with Parafilm. Vapourtec SF 10 pumps, controlled by the automated algorithm, delivered the reagent solutions, water, and solvent (if applicable) at accurate flow rates using PFA tubing DLS measurements were conducted online via the Kalliope software. The particle size measurement mode was targeted at 25°C, with automatic adjustments made to quality, filter, and focus settings. The material was standardized for polystyrene, with water selected as the solvent for refractive index and viscosity calibration.