The new method of acquisition for HCD (Higher Energy Collisional Dissociation) MS2 spectra detection without the carrier channel is called SCoPE X (Single Cell ProtEomics eXclude carrier). This new method of detection with the same number of carrier channel (< 100 cells) shows marginal increase in peptide identification with about 10 % of the total amount of identified peptides but increasing the carrier channel number of cells beyond 200 shows improvement in the peptide identification rate by up to 40 % as compared to the standard SCoPE MS type of acquisition. It can be seen that many more peptide fragments were assigned in the SCoPE X MS mode with the same number carrier channel cells. With larger number of cells in the carrier channel even more peptides are identified from single cell analysis in all our studies as described below.