The CAC for the three self-assembled systems in aqueous solution were determined using the I₁/I₃ fluorescence intensity ratio of pyrene. Absorption and emission spectra were recorded at different concentrations, with the excitation wavelength set to 334 nm and a fixed pyrene concentration of 5.0 × 10⁻⁷ M. Fluorescence spectra were measured at concentrations of the OA-amino acids both below and above the CAC.