Using the C-MST-assay for identifying new tubulin binders and determining their binding sites. We took advantage of the C-MST assay to characterize diverse sets of compounds (Fig. ). griseofulvin-correlating compounds from the NCI-library. e) Results of the C-MST assay screen of compounds partially characterized previously by us or others, and the uncharacterized compounds. Each data point represents the average of 3-4 technical replicates of an independent experiment. Statistical significance was assessed using a one sample Z-test with Bonferroni correction applied for multiple comparisons. ns -not significant; *, **, *** -statistically significant after Bonferroni correction with 𝛼 = 0.05, 0.01 and 0.001, respectively and 32 samples. Dotted horizontal lines mark ± three standard deviations from the control. C-MST assay hits classified as colchicine site binders are shown in red. Compounds that had shown weak effects in vitro and no effects on cell division in cancer cell culture are shown as open circles.