The second study, MTBLS404 , compared urinary profiles with liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) for 183 adults (health status unknown, participants with BMI above 32.9 kg/m2 were excluded). The study included 100 males and 83 females (age range: 40.9 ± 10.3 years); no information was shared on the ethnicity of the participants. In total, 120 metabolites were identified, out of which 30 were found to be related to age, by univariate statistics (Spearman rank correlation test) and orthogonal partial least-squares. For the data visualization, the log2 fold changes (log2FC) for urine from Table of the original paper were used, for which all metabolites related to age were annotated with the provided HMDB identifiers; for 22 compounds, no HMDB identifier was provided. If a compound was measured in positive and negative ionization mode, the average value for the log2FC was calculated.