The decomposition of microcapsules by using sodium citrate and trypsin-EDTA were investigated. Microcapsules encapsulating FITC-dextran (Mw: 2000 kDa) were treated by sodium citrate (150 mM) and trypsin-EDTA (0.25% and 0.125%, w/v) for different time, separately. During treating, the release of FITC-dextran and the morphological evolution of capsules were monitored and imaged.