Ten thousand soybean seeds of Arısoy variety (Glycine max L.) were applied a 0.1% EMS (Sigma Aldrich, M0880-25, Taufkirchen Germany) solution. All the treated seeds along with the wildtype as a control (no treatment) were sown in the experimental field of Akdeniz University (36°53'N, 38°30′E and altitude 33 m) in Antalya, Turkey in 2018. A commercial herbicide named Hammer10 WP (Doğal Kimya Company, Antalya, Turkey) with 10% chlorsulfuron active ingredient was used in this study. Three hundred thousand M2 soybean seeds were planted in experimental field and sprayer with chlorsulfuron active ingredient was applied with a concentration of 10 g da -1 when the plants were at V2-V3 stages. As a result of field trials for two years, one mutant plant in 300.000 M2 population was determined as completely resistant Then, offspring were obtained from this resistant mutant and, the AHAS gene region sequence analysis of resistant and sensitive plants was performed. 197 amino acids found different because of point base mutation (paper is under review) in mutant and wild type sequence analysis. After the resistant was determined, herbicide was applied at doses of -10-20-30 to both sensitive wild type and resistant plants. To confirm the resistance, 10 seeds from the herbicide-resistant mutant named as AntSoy (sourced from the M2 population under field conditions) and 10 wild-type ArıtSoy seeds were planted in 5 L pots and grown in the greenhouse. When the plants reached the stage of V2-V3, the lowest dose to achieve full efficacy (10 g da -1 ) chlorsulfuron was applied in the greenhouse. The resistant progenies of AntSoy were also grown in a greenhouse to determine the resistance level at higher doses. For this purpose, 20 and 30 g da-1 (2X and 3X) herbicides were applied to the mutant progeny along with the wild-type.