One of the factors limiting the characterization of non-equilibrium colloidal assembly such as OM structure is not only their dynamics but also their relatively high packing density which becomes a problem for standard optical microscopy method when the size of the particle's approaches (or are smaller than) the diffraction limit as they cannot be individually resolved. In this section, we demonstrate that our FRAME is also able to characterize optical matter even when formed by particles smaller than the diffraction limit. For this, we used carboxylate-coated fluorescent polystyrene nanoparticles with diameters of 300 nm and 200 nm. After 20 minutes of NIR laser irradiation, a large-scale assembly structure was formed and locked using FRAME methodology. Subsequently, we employed 3D confocal microscopy and SEM imaging to resolve the structure of the resulting permanent optical matter.