Alanine dipeptide is a 22-atom system that is commonly used as a test system for new methods. Initial structure was obtained from ALA2/. TIP3P water model was used to solvate the system explicitly. For GaMD-WE, GaMD was run for 50 ns, and WE was run for 11.95 µs so that the total simulation time amounted to 12 µs. Simulation time, τ for WE was set to 10 ps, equal to GaMD's sampling frequency. The target number of walkers per macrostate n w was set to 4. CVs were set to be the dihedral angles, φ and ψ. Macrostates were evenly spaced in intervals of 10 • for both φ and ψ (ranging from -180 • to 180 • ).