First, we determined the sequence coverage and percentage of fragments explained for each of the three selected spectra. To compare the performance of IsoDec against existing tools, we benchmarked the results of IsoDec against those of TopFD and FLASHDeconv. IsoDec and TopFD produced the highest sequence coverage on all three spectra (Figure ). The two tools performed similarly, yielding between 63-77% sequence coverage across the three spectra. FLASHDeconv achieved from 41-55% coverage.