Steered molecular dynamics were run with Amber20 and PLUMED v2.6.1 via BioSimSpace. The collective variables used are outlined in Supplementary Figures 1. In all cases the first 4 ps were used to apply the force, maintaining the CVs at original values. Open to closed loop conformation sMD was carried out over 150 ns with a 3500 kJ mol -1 force constant, while closed to open sMD was carried out over 100 ns with a 2500 kJ mol -1 force constant. The target values for the allosteric residues were taken from 1 µs equilibrium MD simulations (Supplementary Figure ). All simulations were run at 300 K and 1 atm.