To study the impact of the starting conformation on the geometry of UBP we explored MUDANTI and MUDPAR structures as starting points for the simulations. Similar to the MUDSYN scenario, commencing with MUDANTI also revealed a propensity for random conformational changes of the UBP. Here also WCF and nWCF structures are generated during the simulations where nWCF structures are found to be predominant. Noticeably, in this case outerphase nWCFO structures are not observed. Conformational change between SYN and ANTI further verifies the occurrence of intra-strand flipping, a phenomenon evident through the corresponding O-S distances (Figure ). Conformational change through Inter-strand flipping is also discernible from the UB-NB distance curves (Figure ). Here the pattern of the RMSF looks similar to the one obtained for MUDSYN, underscoring the flexibility of the UBP, which remains dynamic in nature and doesn't depend on the initial structure. Stability of WCF and nWCFP1 structures are confirmed by low RMSD values whereas high RMSD value of distorted structures indicates that they are comparatively less stable (Figure ).