Rescue experiments with 17c via Simple Western™ immunoblot analysis. MM.1S cells were treated with tubastatin A (10 M), pomalidomide (10 M), MLN4924 (10 M) alone or in combination with 17c (1 M) for 24 h according to the depicted scheme. For co-treatments binding-inhibitors and neddylation inhibitor MLN4924 were added 30 minutes before 17c. GAPDH was used as loading control. Representative images from a total of n = 3 replicates.