To confirm the in silico-predicted ability of all compounds to penetrate through the BBB, the parallel artificial membrane permeability assay (PAMPA) was used . The assay validation has been performed using standard compounds whose CNS status is experimentally known from in vivo conditions . The results of commercial drugs and new investigational compounds are summarized in Table . Based on the results, most compounds demonstrated a high probability of crossing BBB via passive diffusion. The derivative 5o exhibited the most favorable outcome. However, permeability predictions for compounds 5h, 5i, 5j, and 5m remain uncertain as these compounds reached permeability values that lie between assay positive and negative outcomes.