To prove the versatility of the proposed method, DOPC liposomes embedding three different RRs molecules were prepared and mixed with the AuNPs dispersion (Figure ). More specifically, we prepared and compared DOPC liposomes containing the same amount (1% mol/mol) of MBA, RR2, and RR6, specifically designed in a previous work to have welldistinguishable Raman peaks in the 1000-2500 cm -1 region. First, we verified that the probes retained their Raman profiles by acquiring several spectra of the different DOPC(RRs)-AuNPs batches (Figure ), where the characteristic peaks for each tag were highlighted. Then, the effect of liposome and RRs concentration, as well as the colloidal stability and reproducibility of Raman and plasmonic signals, were investigated. As reported in the SI (Figure and S5), no significant differences in the preparation protocol were detected when varying the RRs structures. As shown for MBA-containing liposomes, the SERS signal follows the AuNPs clustering-induced plasmonic variations and the interaction can be controlled by varying experimental parameters such as liposome composition and concentration. These results confirm that RR nature doesn't significantly affect the interaction mechanism (see S4 and S5