For this, the first step of FRAME (Figure ) was performed, during which widefield microscopy images were taken before and after gelation, confirming the absence of structural changes. Then, we performed 3D confocal microscopy imaging which successfully resolves the structural properties of the OM in 3D (Figure ). Finally, after drying and sputtering with Au/Pd, the sample was imaged using SEM. Note that the SEM imaging was performed in high vacuum condition, which can result the partial drying of the sample, emphasizing the need for checking that the structure remain unchanged throughout. For this, we correlated the confocal images (before SEM sample preparation) and SEM imaging (Figure ) of the optical matter. We overlaid the two images and quantified the displacement of microparticles (MPs) in the optical matter between the SEM and confocal images (Figure ). The average shift of the particles in the X and Y directions was 43 nm and 33 nm, respectively, with some errors attributed to the use of two different imaging systems with distinct environments, magnifications, and other factors.