As previously mentioned, a time step of 10 fs was used, when simulating the coarse-grained LHA models. This was done because the occurrence of instabilities at 20 fs, which is commonly used for Martini simulations, and higher time steps, made it impossible to observe a trajectory with a sufficient simulation time, such as 500 ns. A more detailed investigation with a high output of frames from the trajectory revealed the parts of the system that are most likely to be responsible for this, for instance, the ones that were connected to the HS30 fragment. These parts showed a tendency towards instability, due to an extreme rocking of the beads within a relatively short time. It can be regarded as a relatively rare event but makes it impractical to run long simulations at a larger time step.