Current approaches for selecting anti-idiotypic reagents for assay development typically follow a general approach where multiple clones that show a positive titer for binding to the drug molecule are selected and further screened . The types of screenings used may vary based on the type of target and overall assay requirements . Epitope binning is commonly used to select optimal antibody pairs, but ideally multiple pairs are selected to avoid instances where performance changes with the assay format and platform being used. Assay format, including homogenous and sequential, can have an impact on overall assay performance and usually are not evaluated during the reagent selection process, especially when automated approaches for sample preparation and data analysis are not available. More comprehensive screening using automation has been demonstrated by Salimi-Moosavi et al. that enables screening of a large number of antibody pair configurations for a traditional colorimetric ELISA . We have expanded this approach and leveraged automation to encompass six different assay formats, multiple concentrations of drug, soluble ligand and anti-IDs.