Simulations were carried out in triplicate, for a minimum 23 µs per replicate, for a total simulation time beyond 2.3 ms (some replicates were simulated significantly longer than others; see Supplementary Table ). The GROMACS 2020 simulation package was used with a Verlet neighborlist scheme, at a 20 fs time-step. Typical Martini settings were used for nonbonded interactions : Lennard-Jones and Coulombic potentials were cut-off at 1.1 nm, with Coulombic interactions further treated under a reaction-field scheme with a dielectric constant of 15. Temperature was kept at 300 K by a V-rescale thermostat with a 1 ps coupling time. A Parrinello-Rahman barostat with 12 ps coupling time was used to maintain pressure at 1 bar; pressure was coupled isotropically and semi-isotropically (independently in the xy and z directions) for the coiled-coil systems and WALP/membrane systems, respectively. After system assembly and energy-minimization, but prior to production runs, a 2 ns pressure/temperature equilibration was run for all systems, using the more robust Berendsen barostat with a relaxation time of 3 ps.