The formation and evolution of silver nanoparticles with time were studied using UV-Vis spectroscopy. To limit the affecting factors on the kinetics of nucleation we used HLNPs prepared only from HL85 and modified the particle size by varying the initial concentration of HL85 and keeping the dispersion concentration of HL85NPs constant in the silver reduction reactions. The reactions were followed by UV-Vis absorbance spectra collected every 10 minutes for 2 hours. There was a hypsochromic shift in the growing surface plasmon resonance (SPR) to a lower wavelength as the particle size of HL85NPs decreased (Figure and). This can be attributed to the difference in the surface to volume ratios of the particles. Smaller nanoparticles have a higher surface area and hence there is a higher number of sites for heterogeneous nucleation of silver. Consequently, with higher number of nuclei available, the silver ions available in the solution will be shared among higher number of seeds and there will be less free silver ions for the growth step. This results in a higher number of silver nanoparticles with smaller size.