To assess the dynamics of the assembly and disassembly process, the turbidity of the solution in response to EDC was followed in a plate reader (Fig. ). While the Fmoc-GGD-based vesicles dissolved to yield a transparent solution as the product concentration decayed, the fibers by Fmoc-AVD did not. After 24 hours, a substantial amount of turbidity was observed for Fmoc-AVD, despite all anhydride having hydrolyzed within the first hour of the reaction cycle. From this observation, it is clear that the precursor remained kinetically trapped in the fibers.