We selected octanoic acid (OA), a medium-chain fatty acid as our model fatty acid. Its CAC is influenced by environmental factors, such as pH and ionic strength , with a critical micelle concentration (cmc) ≈ 0.4 M at high pH (above pH 7) . Molecular dynamics simulation studies have provided insights into its aggregation behavior and molecular interactions , while its temperature-dependent self-assembly behavior has been thoroughly investigated . By conjugating OA with three different amino acids, glycine, threonine, and phenylalanine respectively,, we aim to systematically investigate their critical aggregation concentration (CAC) and self-assembly morphology.