The effect of temperature was especially noteworthy for crystals of Zn(II)-methionine, their sharp intensity changes observed at room temperature were not observed at 100K. Instead, Zn(II)methionine crystals at 100K underwent smooth, prolonged periods of non-monotonic intensity fluctuation akin to those observed in other beam-sensitive samples at room temperature (Fig ). An interesting exception to this trend was noted in a select few crystals of Zn(II)-histidine and the Co(II)-porphyrin, which at 100K exhibited more pronounced anomalous fluctuations than what was generally observed at room temperature (Fig ). These cases contribute to an increased mean fluctuation in values of the derivative of total intensity profiles for these compounds at 100 K with respect to crystals of the same type at room temperature (Fig. ), though it should be noted that the typical behavior of these crystals at 100K was to yield very subtle or entirely absent non-monotonic fluctuations as fluence accumulated (Fig. ). Apparent charging, evidenced by warping of the shape of the electron beam in diffraction mode, and visible bulk translations of crystals when illuminated, limited measurement of Cu(II)-serine crystals at 100K. We therefore could not include Cu(II)-serine crystals interrogated at 100K in our comparative analyses.