The infrared (IR) spectrum of the matrix-isolated sample is collected from a single pass of the externally coupled FTIR beam through the matrix-isolation chamber and sample before being focused into the external MCT-A detector. The FTIR is equipped with a broadband KBr beamsplitter, limiting the spectral range to 7400-600 cm -1 . The spectra are recorded with a resolution of 0.5 cm -1 -which corresponds to a data spacing of 0.241 cm -1 -and background subtracted. Spectroscopic signatures corresponding to each conformer are integrated using OriginPro to determine the cis and trans conformer total absorbances.