Inducing cis-trans isomerization on the benchtop while simultaneously measuring mem-brane properties is exceptionally difficult. However, molecular dynamics (MD) simulation offers a computational microscope for biomolecules, and is uniquely suited to offer insight into well-controlled and precise interrogations into the molecular motions of lipids and proteins. Previous studies into cis/trans isomerization viewed through the computational lens had identified trans-specific interactions with cholesterol. Rather than repeating those studies on model membranes, the P. putida membrane offers a greater diversity of lipid types and unsaturation profiles than prior studies. By comparing the membrane structure and dynamics between membranes composed of all-cis and all-trans fatty acids, we quantify the triggered response to environmental stresses in P. putida noted in prior LC/MS experiments. All-trans membranes are thicker with a lower area per lipid, and diffuse noticeably slower than the all-cis membrane controls. Crucially, the cis unsaturation sites are more exposed to solution, and may be the origin for the increased bioavailability of cis fatty acids in addition to altering the membrane mechanical properties.