The nanostructured Au film was functionalized with a mixed selfassembled monolayer (SAM) of WK3SH and 6MH, which was incubated overnight in a solution of 0.6 mM WK3SH and 0.4 mM 6 MH in 1-propanol. Evaluating the capacitance indicated a WK3SH:6MH ratio of ~70:30 on the electrode surface as required for the formation of tBLMs with 6MH-supported free standing BLM domains. Unilamellar POPC vesicles were prepared according to Schmitt et al. at a final concentration of 0.5 mg mL -1 by extrusion through polycarbonate filters with a pore size of 200 nm in 100 mM NaCl and 20 mM BTP buffer at pH 7.4. The tBLM was assembled by addition of the unilamellar vesicles onto the SAM. After two to three hours, the suspension was replaced by a vesicle-free buffer solution. The formation of the tBLM was confirmed in each experiment by recording SEIRA spectra as well as the capacitance and resistance of the membrane as shown previously. Ethanol-dissolved EB was added to the supernatant buffer solution at a concentration of 100 μM such that the ethanol solution did not exceed 2%.