To address the identified knowledge gaps, we investigate the abiotic transformation pathways and adsorption-desorption mechanisms and kinetics of glyphosate with protein-Mn oxide associations. Experiments were conducted across minerals containing different Mn oxidation states (II, III, and IV) and under environmentally relevant pH conditions (4.6 and 7.2). This research employs in-situ time-resolved ATR-FTIR spectroscopy and microfluidic chamber studies coupled with LC-MS and XPS analyses. Overall, Our findings reveal how glyphosate's interaction mechanism and its transformation pathways are tightly regulated by protein adsorption on Mn oxide minerals.