A fingerprint of the protein corona composition was obtained for samples incubated under various biologically-relevant incubation conditions using SDS-PAGE analysis. We demonstrated that there were changes in protein corona composition when incubated under shear flow as opposed to static conditions across all surface chemistry (unmodified, aminemodified, carboxylate-modified). Unmodified polystyrene nanoparticle data strongly correlates with a study conducted by Jayaram et al. in which similar particles were used, particularly with the trend observed at ~65 kDa where an increase in band intensity is observed at 0.85 cm/s followed by a decrease for corona composition at (8.5 cm/s). A similar change was also demonstrated across all surface chemistries for time-based incubations, which correlates with trends observed for multiple nanoparticle prototypes including co-polymers , and spherical nucleic acids . SDS-PAGE analysis suggests the identity of adsorbed proteins is influenced by the surface functionalisation of particles, with our results showing similar trends in composition to studies performed with the same nanoparticles.