Herein, we report the automated synthesis of [ 18 F]FSPG on the GE FASTlab, one of the most widely used synthesis module for routine clinical radiotracer production. Additionally, we demonstrate how small adaptations to the production process can maximize output for preclinical studies, even when starting from limited amounts of radioactivity. The final radiotracer concentration is highlighted as a central component in the process's optimization. This optimized method reliably produced [ 18 F]FSPG in good radiochemical yield (RCY) and high radiochemical purity (RCP).