Raw MS data of 48 High-pH fractions were searched using MaxQuant search algorithm (Vesion 2.0.3.0) against Uniprot Mouse database (Release version May 20021 containing 25,375 sequences). 10 plex TMT reporter ion MS3 workflow was loaded and used following search parameters. Trypsin as a protease was selected by allowing two missed cleavages, deamidation of Asn and Gln; Oxidation of Met were used as variable modifications and Carbamidomethylation of Cys as a fixed modification. The default mass error tolerance for MS1 and MS2 (4 ppm and 20 ppm) were used. Min of 2 unique+razor peptides were selected for the quantification. The data was filtered for 1% PSM, peptide and protein level FDR. The output protein group .txt files were further processed using the companion Perseus software suite (version 1.6.15.0) . Decoy hits, contaminants, proteins identified by sites and single peptide hits were filtered out. The data was then log2 transformed and T-test was performed between the sample groups and the p-values were corrected using 5% permutation-based FDR to identify the differentially regulated protein groups. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD034055.