These two compounds were found to be extremely potent, presenting IC 50 in the nano-molar range (0.025 and < 0.005 μM for deguelin and rotenone, respectively), coupled with low cytotoxicity to the host cell (11.117 and 7.906 μM respectively). This is the first report of the activity of deguelin and rotenone, and, more broadly speaking, of rotenoids as a chemical class, against intracellular T. cruzi amastigotes despite rotenone being used as a pharmacological tool in several Trypanosoma metabolism studies . This discovery illustrates how the combination of MEMO-based visualizations and the ENPKG workflow enables hypotheses to be efficiently formulated and tested on a natural extract dataset, which can lead to the rapid identification of bioactive molecules. Link to query C) This hypothesis was confirmed experimentally by evaluating the anti-T. cruzi activity of two of the annotated rotenoids, deguelin and rotenone.