The transition temperature of the liposome formulations was determined using a TA X3 differential scanning calorimeter (TA instruments). A 10 μL aliquot of each liposome formulation was loaded into a hermetic aluminium pan. A buffer aliquot was prepared as reference. Each sample was subjected to two heating cycles from 25 °C to 65 °C at a rate of 1 °C/min with the gas flow (nitrogen) maintained between 300 -400 mL/min. The obtained data were analysed using the TRIOS Software.