The surface pressure -area (Π-A) isotherms were determined with a KSV NIMA -Biolin Scientific, Gothenburg, Sweden. The surface pressure was monitored using a paper Wilhelmy plate. The thermostated Teflon ® trough of the effective film area of 765 cm 2 (58 x 15 x 1 cm) was equipped with two hydrophilic Delrin ® barriers (symmetric compression). Before each measurement, the subphase surface was cleaned by sweeping and suction. Then, the lipid or lipid/CyP-OBu chloroform solutions were spread with a Hamilton syringe on the subphase water and left for 60 min to allow solvent evaporation and to reach an equilibrium state of the monolayer.