In the Matyash extraction, the precipitate was neatly deposited at the bottom of the tube after centrifugation and therefore was easily avoided when aspirating the aqueous material. The organic LLE extracts were analyzed by C8 RPC+, requiring the resuspension of the dried organic phase in the method appropriate solution of water:IPA 1:4 (v/v). The analyzed organic fractions showed no evidence of protein in the spectra (in accordance with the literature, where lipid analysis from LLE has never indicated the presence of protein). In the less frequent cases where the aqueous phase is analyzed, HILIC methodologies are used, requiring further addition of MeCN to the aqueous extract . In both cases, the use of organic solvents plays a protective role by ensuring protein precipitation. This is not necessarily the case in the RPC analysis of the hydrophilic fraction. To mitigate these procedural issues and allow completion of the comparison experiments, all hydrophilic LLE extracts required an additional protein precipitation step using cold MeCN in the 1:3 (v/v) sample:solvent proportion. The summary of our observations during sample preparation can be found in Table .