To scrutinize the binding of PB-inhibitors to PLY several mutants of PLY were constructed. Initially, two crucial amino acids from oligomer-formingregion, E151 and D205, were mutated by site-directed mutagenesis, and the proteins PLY-E151Q and PLY-D205R were expressed. The two mutant proteins were inactive in the hemolysis assay, presumably as protein-oligomerization was affected, however, both featured binding with PB-2 in the biolayer-interferometry assay (BLI) (Supplementary Figure and).