Lipid bilayer used in the study consisted of in the sequence of native UL49.5. In the next step, the protein was positioned in a fully hydrated bilayer followed by the minimization and MD simulations. We performed MD for the UL49.5 protein from both viruses, i.e. BoHV-1 and HSV-1. For each of the proteins, we energyminimized the systems, ran equilibration to obtain proper density and temperature, then conducted three independent 1000 ns simulations (trajectories) for each of the proteins, including two variants of HSV-1, e.g., with charged and uncharged His67. At the beginning we checked what is the minimum distance between the periodic images of the proteins (Figure )