To tune the time delay of the binary-mixed DNA droplet division, we introduced L † AB in addition to the original DNA linker, LAB (Figure ). We describe this binary-mixed DNA droplet as "A:B-droplet," where ':' (double dots) indicates that YA and YB are crosslinked with two DNA linkers, LAB and L † AB. A:B-droplets divide only when both LAB and L † AB are cleaved. In addition, linkers and triggers with " †" indicate those that can achieve a time delay in the presence of inhibitor RNAs and RNase H (Figure ). LAB is cleaved by the active division triggers TABi (i=1, 2), while L † AB is cleaved by active division triggers T † ABi (i=1, 2). Inhibitor RNAs R † ABi hybridize with T † ABi, and form inhibited division triggers iT † ABi, inducing the time delay of A:B-droplet division. This time-delay circuit was inspired by intracellular time-delay control via reaction suppression based on small RNA expression . For such biological meaning and applications shown later, we used natural miRNA sequences, miR-6875-5p and miR-4634 , for R † ABi sequences, respectively (Supplemental Table ); that is, if either of the miRNAs exist, the A:Bdroplet division is delayed.