Continuing our exploration of the membrane structure, we notice more commonalities with previous reports. The double bonds at the unsaturation sites introduce a kink in the lipid tail, particularly for cis stereoisomers. By measuring the angle made between the terminal carbon, the kink, and the carbonyl carbon, we note that the kink is around 15 • greater in magnitude for cis lipids compared with trans (Fig. ), analogous to prior reports. This manifests with less order for unsaturated cis fatty acids compared with trans fatty acids, both for typical phospholipids (Fig. ), as well as cardiolipin (Fig. ). The greater order for trans fatty acids changes the phase behavior for membranes in general, and is associated with transitions from a disordered L d to a more ordered L o or L β phase, with all the accompanying changes in membrane properties.