As these Lys residues are located around acidic patch, we hypothesized that acetylation of these Lys residues may affect histone interactions with acidic patch binding proteins. We examined the binding affinity of LANA peptide, a representative acidic patch binder, to acetylated mono-nucleosomes. EMSA assay showed that H2BK43ac, but not H2BK108ac or H2BK120ac, significantly increased the binding of LANA-FITC to nucleosome (Figure ). These data demonstrated that effects of histone acetylation on biochemical properties of nucleosomes were variable among three Lys residues, and the impact of H2BK43 acetylation was higher than others.