In summary, we present a straightforward method to translate the turnover of enzymatic reactions into the NIR spectrum and amplify the optical signal by using a nanosensor. To this end, we used as model system tailored SWCNTs, which are sensitive to different substrates and products used in typical ELISAs and western blots. Conceptually, this presents a novel approach for a signal translation to a different spectral region with a non-linear signal enhancement that is applicable to any fluorescent sensor/probe. Most importantly, the nanosensor amplifies the signal compared to absorption-based read-outs of colored products. This way it is possible to follow enzymatic reactions in the NIR below the normal limits of detection. The nanosensors can be easily added to and enhance the signal. Therefore, this approach can boost the performance of analytical techniques in which low detection limits of the enzymatic substrates and products are desirable.