Microscaling the previously reported leukotriene-like formation by oxidation of 15-HETE described by Rector et al. and retrieve the partially reported information (MS/MS data) could be an answer to redeem annotation of oxylipins uncovered in past studies. Furthermore, solvent-assisted rearrangement of PGH1 and PGH2 was microscaled with the hope of acquiring the MS/MS data of the main produced metabolites, i.e., the 15-levuglandins LGE1 and LGE2 . Microscaled V70 oxidation of commercially oxylipins. A solution of 30 µL of V70 (0.15 mol/L) in CH3CN was added to 50 µg of 15-HETE redisposed in chromatographic glass vials with 250 µL insert with conical bottom and quickly rotated with small angle using a Bio PTR-35 360° Multi-Functional Tube Rotator from Grant Instruments, Royston, UK) at 40 °C for 24 h in an oven. The agitation conditions were the following: 100 rpm orbital, a reciprocal rotation of 11° turning angle for 6 seconds followed by a 2° vibration for 1 second. The solution is then evaporated with vacuum concentrator and diluted in 100 µL of mobile phase before LC-MS injection. PGH rearrangement. Commercially available standards PGH2 (50 µg) and PGH1 (25 µg) were placed in chromatographic glass vials with 250 µL insert with conical bottom, dissolved in 30 µL of DMSO , and mechanically agitated 1 hour at 37 °C using the tube rotator with the same parameters of microscaled V70 oxidation. Finally, 270 µL of mobile phase were added immediately before the LC-MS injection.