The refinement performance with the new sampling protocol with the original initial model and additional alternative models were benchmarked and compared with our previous refinement protocols. For the comparison, a protocol, named CASP12-simple, which is similar to the protocol during CASP12 or the conservative protocol used during CASP13 , was used as a reference for MD simulations with harmonic restraints on Cα coordinates and at a temperature of 298.15 K. Another protocol, named CASP13-simple, was also used as a reference protocol for using MD simulations with flat-bottom harmonic restraints on Cα coordinates and again at a temperature of 298.15 K. The CASP13-simple protocol is a simplified protocol of the iterative protocol used during CASP13. The key differences were that the iterative sampling part was omitted and RWplus scoring was used for the ensemble selection step. A new sampling protocol, named CASP14, adopted the combined restraints and performed MD simulations at 360 K. For the new sampling protocol, we tested a single initial model-based protocol, which uses the original initial model, and multiple initial model-based protocol, which uses the original initial model and additional alternative initial models.