This study reports mesoporous silica's successful synthesis and functionalization for controlled molecule delivery applications. The synthesized materials were thoroughly characterized. Crystal Violet dye was used as a model molecule to examine release activities at room temperature, controlled temperature, and various pH values. Notably, a significant difference in release was observed between pH 7.5 and 5 for MCM-ZD and MCM-Z nanoparticles. As expected, dextrangated MCM-Z exhibited higher delivery at pH 5 more than MCM-ZD due to the pH-responsive nature of the incorporated molecules. Interestingly, the release profiles at room and controlled temperatures were comparable. This work presented a successfully functionalized MSN with succinic anhydride in 1,4-dioxane, achieving the desired level of surface charge for the first time.