Positive B22 values indicate repulsive interactions, minimizing aggregation, while negative values of B22 indicate attractive interactions that promote aggregation. B22 can be qualitatively assessed by examining the concentration series depicted in Figure . The observed trend of a decreasing hydrodynamic size with increasing concentration suggests the presence of repulsive forces, as it indicates an increase in diffusive motion. Consistent with the B22 observations, the DLS interaction parameter kD, which is derived from the slope of the plots in Figure , is positive in the samples with 10 mM CAPS pH = 11.2 and close to zero in the samples with 10 mM HEPES pH = 7.4. This is interesting, because one would expect greater electrostatic repulsion in the lower pH samples in which KI8 is charged; however, electrostatic screening and possible specific buffer effects are at play, to be explored herein.