Figure shows the fluorescence microscopy images and the fluorescence intensity profiles for SOµC-Bdpy1 after 1 h to 12 h incubation in the GSH solution (λex = 488 nm, λem = 498-531 nm). As for the Bdpy1-GSH conjugates at the surface of SOµC, λex = 560 nm and λem =565-632 nm were chosen. By increasing the time from 1 h to 12 h, the enhancement of the fluorescence intensity in λem =565-632 nm is clearly observed with a reduction, which is also evident from the fluorescence intensity profiles. This observation results from the kinetics inherent to the GSH conjugation process away from the equilibrium, which is consistent with the nature of Bdpy1 as an irreversible GSH probe.