With the multilayered vascular scaffold successfully fabricated, the next phase involves thorough characterization. The robust resistance against varying blood pressures, and the prevention of leakage are foundational requisites for functional vascular grafts . Equally vital is elasticity, ensuring resilience under physiological cyclic loading without dilation. Host vessel compatibility, ensuring an anti-thrombotic lumen through autologous endothelium, adds another layer of complexity . The ultimate tensile strength represents the maximum force the graft can withstand before breaking. This parameter is essential for evaluating the graft's mechanical integrity and performance under physiological conditions. By conducting tensile strength testing, the study aimed to determine and compare the mechanical suitability and structural integrity of the hierarchical structure, mimicking the human internal mammary artery. The findings are particularly significant in the context of target application, which is the design of vascular graft scaffolds for human mammary artery. Remarkably, the obtained mechanical properties of these scaffolds, even with the incorporation of PEG, surpass those of native arteries.