Next, the ditopic diazene aldehyde was screened with the same selection of tritopic amines as computationally modelled (Table ). The same conditions were used as for the previous screen with the tetratopic diazene aldehyde. In all combinations, Tri 2 Di 3 cages were formed as indicated by HRMS. However, only the combination with tris(2-aminoethyl)amine (TREN) formed a Tri 2 Di 3 cage in high conversion as indicated by 1 H NMR analysis, with the other combinations containing large amounts of insoluble precipitate and residual aldehyde.