Details relating to assay components, test strip specifications, and operating procedures for particular experiments can be found in the Supplementary Information. The following is a typical protocol for 'capture-and-release' AmpliFold experiments: To a microplate well, assay components (antigen, FabHER conjugates, nanoparticles) were added and premixed (5 min). To each well, printed capture strips were added and allowed to wick (5 min). Strips were added into wells containing wash solution and allowed to wick (5 min).