Acifluorfen could not be quantified due to co-eluting interferences. All analytes affected by poor repeatability with the comparison method showed instrumental repeatability ≤ 14% RSD in injection solvent at the same concentration in vial. Therefore, with a median instrumental imprecision of 3 % RSD across analytes, the additional variance of the SAX-SPE method was by comparison much lower. The larger imprecision associated with the WAX-SPE approach is due to compound specific low ionisation efficiency and signal suppression from co-extracted matrix. However, by employing a more sensitive targeted multiple reaction monitoring, the overall imprecision of both methods is expected to decrease significantly. Matrix effects (mainly as ion suppression) for the proposed method herein were 7 % lower and more consistent on the SAX-based method (12 ± 9 % versus 19 ± 20% for WAX, expressed as mean ± standard deviation, n=3). Within the limitations of the experimental design and choice of analytes, silica-based SAX still showed promising performance overall when compared to mixed-mode SPE with a WAX-based resin.