This study served as an assessment of the feasibility of the acetylation capabilities of Aspirin-NHS. Our focus was on investigating its potential as an acetylating agent within our model system of β-conglycinin. Through this, we aimed to assess the feasibility of inducing lysine residue acetylation and speculate on its potential implications for NF-κB. Specifically, we aimed to hypothesize the potential effects of RelA acetylation mediated by Aspirin-NHS on specific lysine residues, which could subsequently influence NF-κB functions. Although direct examination of NF-κB was not feasible in this study, we aimed to elucidate the mechanism of lysine acetylation through Aspirin-NHS within our experimental framework.