Based on this rate, numerical calculations would suggest that 80 counted e -/pix/s should be adjusted to ~96 e -/pix/s, to account for CL. Although an ~2 e -/pix/s standard deviation is associated with this correction, that degree of uncertainty is lower than the magnitude of the error due to potentially lost counts (Figure , Supplementary Figure ). These calculated adjustments were applied to raw measured pixel counts, creating CL-adjusted datasets with increased electron counts.