In this study, we successfully demonstrated the enhanced tumor localization of bacteria using aptamer-assisted targeting. We designed in silico aptamers using computational tools and then docked these structures onto bacterial proteins. Two sets of aptamers from the previous research have been taken in this research: AS1411 and ULS11a. Later, mutant forms of these aptamers AS1411_M1, AS1411_M2, and AS1411_M3 for AS1411 and ULS11a_M1 and ULS11a_M2 for ULS11a were developed. The DNA-based aptamers and their respective mutants () dot and bracket notations are shown in Table . Understanding the surface chemistry of the protein has dramatically increased the understanding of protein aptamer binding functions. Firstly, GrASP utilized binding site prediction using GNN analysis. Figure shows the binding site. The binding site is displayed in yellow and green. However, in this figure, the aptamer cannot access it because the site is too small. Figures and show the receptor's ESP estimated with ChimeraX. It provides a look into the nature of protein interactions with other molecules. Positives are blue, negatives red, and neutrals white.