unphosphorylated wild-type and G159D. The most significant change from the WT is the formation of a strong, ~80%, ionic interaction between Arg83, at the bottom of the D-helix and the Asp159 The Arpeggio analysis reveals interactions between the C-terminus of cTnT and both cTnC and cTnI not previously noted (Figures ). There is a medium interaction formed between the NcTnC EF1 hand, Asp33, and the CcTnT Arg286 upon phosphorylation which correlates with the repositioning of the cTnC Lys39 and the phosphorylated serines described previously.