Hybrid hydrogels endowed of shear thinning properties to be injectable through fine needled and capable to rapidly recover their mechanical properties with formation of a solid depot have been engineered for sustained delivery of corticoids, immunosuppressive peptides and small molecules (bimatoprost), in challenging anatomical sites such as the inner ear or the vitreous cavity. Non-covalent interactions enabled the polymer chains to stably entrap drugloaded nanocarriers allowing prolonged exposure to the active molecules. For instance, a single intravitreal injection, in a rabbit model, of bimatoprost-loaded PLA-b-PEG NPs embedded in HPMC-C12, maintained measurable levels of the drug in vitreous humor for up to 8 weeks. Interestingly high-shear thinning hybrid materials have also shown practical application for intracardiac delivery in a large animal model (i.e., sheep) with infarcted myocardium using a clinical catheter. This provided evidence of the real-life potential of materials that had previously shown positive results in rodent models. The excellent injectability of ALG has enabled to apply with a minimally invasive procedure an ALG/liposome hybrid hydrogel to bone defects with different geometries, enabling it to be used as a bone grafting material. By loading the matrix with a bacteriostatic drug (Van) and liposomes with angiogenic and osteogenic molecules (i.e., DFO and Dexa) the resulting hybrid hydrogel outperformed the commercially available Bio-Oss, a bone substitute material widely used in regenerative dentistry for filling purposes, in terms of vascularized bone formation.