Imaging-mode conditions at 200 kV, using the Talos F200C and DE Apollo detector, were configured to match the flux and illuminated area size used to illuminate stationary crystals in diffraction mode. The condenser lenses were set to C1 = 41.317% and C2 = 45.449% at spot size 11, identical to what was used for diffraction mode at spot size 11. A magnification of 4300x was selected, as at this magnification the beam under this lens configuration fully encompassed the area of Apollo detector sensor. Low dose presets were configured in serialEM, such that "Record" mode loaded these imaging settings, and "Search" mode loaded the equivalent-flux diffraction mode settings. Crystals dry-mounted on TEM grids at room temperature were located on a low-mag (155x) atlas, then imaged in cycles, where a single 1 second diffraction movie was collected followed by a 25 second imaging movie. In each mode, the detector was set to integrate 30 frames, such that each movie consisted of frames (2 per diffraction movie, 50 per imaging movie) integrating over 0.5 second exposures. Simple serialEM scripts were written to reconfigure the microscope optics and camera acquisition settings between each exposure while keeping the beam blanked at all times when not acquiring data. The beamstop and 100 μm selected area aperture were inserted manually prior to each diffraction movie. These were retracted, and a 100 μm objective aperture aligned with the unscattered beam inserted, prior to each imaging movie. Ten such "cycles" were performed for each crystal studied.