This study advances the field by employing an innovative approach that integrates metabolomics, microbial sequencing, and silymarin catabolite analysis. Additionally, we demonstrate how age can influence the metabolism and potential efficacy of dietary polyphenols, a perspective not previously explored. Our findings open new pathways for agetargeted dietary interventions and deepen our understanding of the complex relationships between diet, microbiota, and metabolic health. CZ.02.01.01/00/22_008/0004597). For the purpose of Open Access, a CC-BY public copyright licence has been applied by the authors to the present document and will be applied to all subsequent versions up to the author-accepted manuscript arising from this submission.