The ratio for the sample to the KBr used is 1:100. Take two stainless steel discs from the desiccator and place a piece of cardboard between the plates followed by the addition of thymoquinone ground with the KBr. Place this sandwich into a hydraulic press with a pressure of 100psi to obtain a neat clean pellet. Transfer this pallet into the sample loading spot of the machine and run the software to obtain a spectrum.