The chloroform /methanol (1:1) crude extract (12 g) was adsorbed on equal amount of silica gel and subjected to silica gel column chromatographic separation (150g silica gel) and eluted with increasing gradient of ethyl acetate in n-hexane. A total of 105 fractions were collected and each fraction was monitored by TLC plate (Table ). Fractions that showed similar RF values and the same characteristic color on TLC were combined.