PBS buffer containing enzyme at 25 units/ml (pH 7.25) was prepared and sodium azide (POCh, Gliwice, Poland) was added to the solution at a concentration of 0.05% to prevent bacterial growth . The degradation medium was changed every 48 hours to maintain enzyme activity. The samples were placed in polystyrene microplates and kept in dynamic conditions by shaking them in an orbital shaker at 37°C. The degradation study was measured at 6 different time points (days 1, 2, 5, 10, 15, and 20) with 4 repetitions for each copolymer at each time point. After each time point, the samples were collected, rinsed with distilled water, wiped with a cloth, weighed accurately, transferred to a vacuum oven for 10 days, and weighed again. The weight loss percentage (D) and swelling degree (S) (water uptake) were calculated using the following formulas (1) and ( ):