While our simulations are performed with only a single copy of a structure, practical self-assembly involves synthesizing multiple copies at once. However ɑ(T,ε') should be similar regardless of the number of copies, as all interactions between blocks are identical between the two except for the possibility of self-interaction in the latter. We confirmed such behavior through simulations of nine copies of the N = 10 chain, which displays a similar ɑ(T,ε') = 1 domain as that of a single copy of the chain (Figure , Table ). Moreover, experiments often create "closed" structures, as opposed to the "open" structures (such as chains, and shapes without closed loops) we have thus far examined. The assembly outcome of such closed structures is harder to predict, with success highly dependent on the sub-structures that develop during the synthesis pathway. For example, along the pathway to forming a 3X3 square tile typical of DNA tiles, smaller correct structures can form, but are unable to join together due to steric hindrance (Figure ). Nevertheless, we observe that closed structures such as the 3×3 tile also exhibit a broad triangular domain of ɑ(T,ε') = 1. Thus the behavior from our CG-MD results should be broadly applicable to practical selfassembly experiments.