PaFabF C164Q was crystallized as described before. Purified PaFabF C164A (8 mg/mL) was mixed with well buffer (35% PEG 3350, 0.24M ammonium format) at 0.6:0.3 µL drop ratio and let to equilibrate at 25 °C using the vapor diffusion, sitting drop technique. Crystals were observed within 12 hours and crystals growth continued for up to 2 days.