Table . Mass of 2NapFF and volumes of hydroxide and water needed to make 1 mL of various concentrations of 2NapFF solutions. Asterisks represent when 1M hydroxide was used to make the 1:1 XOH:2NapFF ratio possible. 3 mL of each concentration of 2NapFF solution (5-100 mg/mL) were prepared using the quantities in Section 1.3, using NaOH or TBAOH. Solutions were made in Sterilin vials, wrapped in Parafilm, and left to stir overnight at 1000 rpm using 13x3 mm stirrer bars. The following day, once a homogeneous solution was present, the pH was adjusted to 10.5 ± 0.1 using 0.1 M, 1 M and 2M of the corresponding hydroxide; or 1 M and 2 M HCl as required. All solutions were prepared in this way, unless specified.