Datasets were imported with the following Lipostar parameters: 5 ppm m/z tolerance, peak intensity > 1% base peak intensity, peak detection frequency > 1% and a minimum spatial chaos > 0.7. Ion images were displayed as TIC normalised and visualised with the viridis colour settings (except when ion images are overlayed) with hotspot removal (high quantile of 99%) and no linear interpolation or denoising applied.