For both BLUES and MD, the gauche(-) rotamer is the dominant state regardless of the starting point; however, the increase in the Val111 torsional barrier post-backbone relaxation is reflected in the relative increase in occupancy of this state. For BLUES, the relative occupancies of the gauche(-) conformation for T0, T250, and T1000 are 0.58, 0.78 and 0.88 respectively (with not enough data yet collected for convergence). By comparison, the relative occupancy of the gauche(-) rotamer state for the MD simulations is 0.97, 1.0, and 1.0, though the occupancies computed from MD are even further from convergence than those from BLUES since the number of transitions is far lower. Overall, it seems that some slow protein relaxation is impacting at least the likelihood of rotamer transitions, but potentially also the relative preference of different rotameric states.