Liver homogenates, mouse kidney and the tumour bearing liver tissues were sectioned at 10-15 µm thickness using a cryo-microtome (Leica, Nussloch GmbH, Germany) at -20 º C and thaw-mounted onto microscope glass slides. Following sectioning, samples were stored at -80ºC until required. Once required, all tissue sections were coated with NEDC matrix using an automatic sprayer (TM-Sprayer, HTX Technologies, LLC, Chapel Hill, NC, USA). NEDC matrix was dissolved in a 7:3 mixture of MeOH:Water (v:v) at a concentration of 14 mg/mL. pressure, nozzle temperature of 80 º C and a 1200 mm/min velocity. Matrix density is reported at 0.003500 mg/mm 2 .