Once the native structure(s) are established, native contact pairs are identified as particles which are less than a distance cutoff c cut from each other. Then, the pairwise distances between native contacts in the native structure (r native ) are used to compute the fraction of native contacts (Q) of each uncorrelated frame in the replica exchange trajectory set. A multiplicative tolerance factor (c tol ) is applied when scanning the trajectories for native contacts. Thus, a pair is considered a native contact if 1) it is a native pair defined in the native structure, and 2) the distance between two particles is less than or equal to r native c tol . Using MBAR, the expectation of Q as a function of temperature is computed from the reduced energies evaluated at all thermodynamic states of interest and the corresponding native contact fractions. In a well-defined foldamer system which transitions from a disordered state to a single folded state, the Q vs T data is well-described by a sigmoidal hyperbolic fitting function: