With the design of our prototype system validated, we installed the heterogeneous sample analysis method on all our walk-up LCMS instruments. This proved to be the critical phase of this project, as these systems are closely monitored for performance metrics with comprehensive records compiled featuring details on time and reason for an instrument being out of service. Instrument performance has been constantly under evaluation since 2010, and Figure provides a snapshot of the number of instances of "instrument clogging" over a four year period, with the back-flush being implemented and rolled out to the six walk-up LCMS sequentially over a 10 month period. We felt that this provides a representative body of data to provide an assessment of the impact of the change to our operations. It is important to stress that over this period, the number (and identity) of the users and the types of chemistries evaluated (typical medicinal chemistry reactions) has remained approximately constant, while the number of samples run has increased. None of these instruments have either been replaced or undergone significant upgrades in the time of review, and each instrument typically hosts 6-8 users depending on its specific location within the building. The one exception herein is the placement of one of the instruments in a controlled high potency laboratory, which is used on a far less frequent basis than our other systems (vide infra). Given this, there were no extraordinary events (such as taking instruments out of commission either completely or for an extended period of time during the evaluation) at the time of the heterogeneous sample analysis method implementation shown in Figure that could have also had such a sizable impact on the trends observed.