Here, we have densely coated a CdSe/CdS based QR with DHLA-EG11-DiMan ligands to make the first glycan-QR probe for DC-SIGN/R related MLGIs (Figure ). By developing a QR-FRET based ratiometric MLGI affinity readout, together with hydrodynamic size and S/TEM analysis of lectin binding induced QR assemblies, we show that QR-DiMan with distinct glycan displaying curvatures can effectively discriminate the MLGI properties between DC-SIGN and DC-SIGNR. DC-SIGN binds strongly and simultaneously to one QR-DiMan regardless of surface curvature with sub-nM 𝐾 d , comparable to that with QD-DiMan. In contrast, DC-SIGNR binds simultaneously to the glycans displayed on the central cylindrical section of the same QR but cross-links between glycans displayed on the spherical ends of different QRs. This result demonstrates that the QR's unique geometry together with its strong fluorescence and high EM contrast allows us to reveal how glycan presentation, induced by scaffold curvature, affect MLGIs.