The second step of FRAME is the highresolution characterization of the locked, non-equilibrium assembly, in this case, OM. We demonstrate that making the colloidal assembly permanent enables its further structural elucidation using 3D confocal imaging and/or SEM. However, since FRAME involves photopolymerization and post-processing the sample (in case of SEM), we need to ensure that these processes do not affect the created assembly. To achieve this, we utilized 1 µm fluorescent carboxylated polystyrene (PS) microspheres as their size is significantly larger than the diffraction limit of optical microscopy.