DiPaC-SLIC is based on long amplification PCR combined with homologous nucleotide overhangs which allows for in vitro DNA assembly via SLIC of the BGC directly into the selected expression vector, in this case pET28-ptetO::gfpv2. DNA extraction from M. aeruginosa LEGE 91341 and genome analysis was described previously. The first step of the cloning strategy was to identify putative terminators in the mic BGC using the ARNold tool, but none were detected. Because after several attempts, we were unable to amplify the entire 25 kb mic BGC, we split it in two fragments (Figure ) to facilitate their insertion into pET28-ptetO::gfpv2.