Two wastewater treatment plants (WWTPs), corresponding to two catchment areas, were selected for this project. This study has previously been reported in . Both sites are within the Southwest of the UK and are a close distance from each other (figure ). First, is the city of Bath with a population of 120,113 and second is a small town called Keynsham, population estimation of 21,247. Both sites have limited contribution from industry waste (<1 %). Bath, a UNESCO heritage world site, is popular with tourism, and has two Universities residing in its catchment area. Keynsham resides between Bath and the city of Bristol and so observes less tourism in general but will likely have a commuter input. Influent (untreated) wastewater was collected using 24-hour composite flow proportional samplers for this project. Samples were collected every 15 minutes over the 24-hour period with composite samples combined, to create one 24-hour sample, before being transported on ice to the laboratory for sample processing (<1 hour). Wastewater samples were collected over a 13-month period between 2018-2019.