Based on the molecular structure of 4ABA and the Raman evidence, both the amino and carboxyl functional groups appear to interact simultaneously with the metal surface, suggesting a horizontal binding orientation on the AuAg NP surface. Unlike adsorbed 4MPy, no significant difference was observed in the TE and TM SERS profiles of adsorbed 4ABA, which could be due to the planar orientation of the molecule compared to the upright orientation of adsorbed 4MPy.