Liposomes were synthesized by cold extrusion. A mixture of 70% DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) 30% cholesterol was prepared in chloroform, next, the solvent was evaporated by rotatory evaporation at 40º C with 475 mbar, followed by 0 mbar, led to formation of thin film of dried lipids. In the next step, the dried lipids were rehydrated in PBS buffer and the suspension was overnight shaken at 500 RPM at room temperature. After that, 4 freeze-thaw cycles were carried out in liquid nitrogen. A polycarbonate filter (100 nm) was applied to extrude liposomes over a manual extruder (Avanti polar lipids. USA) in 25 cycles at room temperature. The liposomes were characterized by dynamic light scattering (DLS) with a mean diameter of 101.7 ± 27.7 nm and polydispersity index of 0.074, at 23º C with an intensity of 300 KHz.