Next, we analyzed the influence of the fuel concentration. Low fuel amounts lead to short-lived droplets, offering less time for the long PSS to mature and form speckles (Fig. ). Indeed, we found that the coacervates fueled with 25 mM EDC did not show division. Fueling with 50 and 75 mM EDC leads to, on average, eight offspring droplets. Surprisingly, using 100 mM EDC or more decreases the offspring to roughly three (Fig. ). We assume that with the increasing lifetime of the droplets, the chance that speckles combine increases, leading to fewer offspring. The amount of EDC we used did not influence the offspring's lifetime (Fig. ). Next, we kept the total PSS concentration constant at 20 mM as expressed as monomers but varied the fraction of long PSS from 0% to 65%. Increasing the fraction of long PSS leads to the formation of increasingly more viscous droplets, as measured by FRAP experiments (Fig. ). We observed that droplets without long PSS dissolve homogeneously without producing any offspring (Fig. ). In contrast, droplets with as little as 10% PSS displayed a few speckles and consistently produced droplet fragments, albeit only a few. With an increasing fraction of PSS, the number of offspring increased. However, when we tested 40% long PSS or more, the fusion of the droplets was so slow that the initial droplet was rugged and inhomogeneous. At 65% of long PSS, it became evident that the large droplet was merely a conglomerate of the droplets but so viscous that they did not fuse. While the dissolution led to droplet fragments, it was clear that the fragments were simply the remnants of the original, unfused droplets. Given that the original droplets were never homogeneous, we argue that this process is hard to define as division. We also analyzed the offspring's lifetime, which increased with increasing ratio of long PSS from an average of 40 seconds to 70 seconds from 10% to 35%, respectively (Fig. ). Finally, we tested the length of the long PSS on the droplet division behavior and found that a long PSS of 261 kDa at 25% did not produce any droplet fragments. Lengths beyond 261 kDa consistently produced offspring with nearly identical lifetimes (Fig. ). We conclude that the division mechanism is a concerted effort between the short and long PSS-the short is required to produce liquid droplets that enable the long PSS to mature in the next generation of droplets. Too much short PSS hardly produces offspring. Too much long PSS prevents the fusion into the necessary droplet for maturation.