At the moment the most commonly used hydrophilic polymers on the market for biomedical applications are poly(ethylene glycol) (PEG), poly(N-isopropyl acrylamide) (PNIPAAM) and other polymers which contain acrylic or methacrylic acid, partially hydrolysed poly(vinyl acetate), poly(vinyl alcohol) or poly(vinyl pyrrolidone). Poly(2-oxazoline)s (POx) are intensively investigated as alternative biomaterials, with one member of the family, poly(2-ethyl-2-oxazoline) being FDA approved as indirect food additive. POx are a class of polymers obtained via (living) cationic ring opening polymerization and because of structural similarities to poly(amino acids) they are sometimes also called pseudo-polypeptides or bioinspired polymers . Furthermore they exhibit good thermal stability (decomposition temperature typically > 300 °C) and are typically considered safe biomaterials, which makes them promising materials in medical applications in general and hydrogels in particular . Therefore, in recent years they are intensively investigated for these purposes. Several strategies to obtain poly(2-oxazoline)s containing hydrogels can be found in the literature. Very early, Chujo et al. reported in a series of groundbreaking studies on chemical crosslinking of 2-methyl-2-oxazoline (MeOx) and 2-ethyl-2-oxazoline (EtOx) by Diels-Alder reaction, disulfide coupling and other reversible reactions . Wang and Hsiue reported the crosslinking of PEtOx dimethylacrylat with three-arm poly(D,L-lactide) trimethacrylate by photopolymerization . Dargaville and co-workers reported the mild photo-crosslinking of copolymers of 2-methyl-2oxazoline (MeOx) or 2-ethyl-2-oxazoline (EtOx), respectively, and 2-(dec-9-enyl)-2-oxazoline (DecEnOx) with dithiol crosslinkers and investigated the swelling properties of the resulting monolithic hydrogels . Dargaville also reported on photo-crosslinking of water soluble copolymers of 2-methyl-2-oxazolin and 2-(3-butenyl)-2-oxazolin (PMeOx-co-ButenOx) as well as copolymers of 2-methyl-2-oxazoline and 2-undecenyl-2-oxazolin (PMeOx-DecenOx) copolymers using a low molecular dithiol . Later, Hartlieb and co-workers reported on crosslinking copolymers of EtOx and 2-(5-amino valeric acid)-2-oxazoline and the resulting swelling properties of the hydrogels as well as their ability to absorb and release DNA . Zahoranova et al., reported the synthesis of PEtOx based hydrogels with different bis(2-oxazoline)s by "one pot" random copolymerization . All those reports dealt with chemical crosslinking. Lorson et.al. reported on thermogelling block copolymer based on the hydrophilic poly(2-methyl-2-oxazoline) (PMeOx) and the thermoresponsive poly(2-n-propyl-2-oxazoline) (PnPrOx) which is an interesting material as bioinks and biomaterial inks for three-dimensional (3D) cell culture and biofabrication . Haigh and coworkers reported on UV crosslinking of poly(EtOx-co-2-(3-butenyl)-2-oxazoline) for creation interconnected pores inside resulting hydrogel's meshes with potential application cell scaffolds or drug delivery systems . Overall, POx based hydrogels have seen a surge of interested in recent years and recent reviews by Kelly and Wiesbrock as well as Dargaville and co-workers provide an excellent overview .