Compared to the SHEATH-sudden-ZF sequence, the NMR signals obtained after application of the SHEATH-adiabatic-ZF sequence are 30% smaller (e.g., integral of the 2-J peak is approx. 35 vs. 27 pT). This reduction occurs because maintaining adiabaticity during the field variation step requires employing a relatively long ramp for [ 15 N]-ACN, whose Jcoupling is only around 1.7 Hz-orders of magnitude smaller than the typical single bond C-H J-couplings(∼ 140 Hz). During this ramp, the sample experiences relaxation that lowers observable signals. In our work, a ramp time of 869 ms was employed as a compromise between achieving good adiabaticity and minimizing relaxation effects.