To evaluate the impact of the storage at -80 °C on cell metabolites, we performed comparisons between Group 1 and Group 3 as well as between Group 2 and Group 4. We discovered increased (58) and decreased (134) metabolites in the comparison of Group 1 vs. Group 3 (Figure ). Pathway analysis based on tentatively labeled metabolites did not reveal any pathway significantly impacted (Table ). The comparison between Group 2 and Group 4 showed 144 increased and 100 decreased metabolites (Figure ). Pathway analysis based on tentatively labeled metabolites revealed that galactose metabolism and starch and sucrose metabolism were significantly impacted (Figure , Table ).