From the top 500 compounds, we further selected and were able to synthesize 50. Testing of these synthesized compounds revealed that 21 exhibited measurable activity, as indicated by negative curve class values. Further scrutiny within this active group identified 6 compounds with IC50 values below 30 µM and efficacy (Eff) values of less than -50%. The most potent compound within this selection stood out with an IC50 of 35 nM, showcasing the strength of our integrated approach in discovering highly active compounds for potential therapeutic use.