Moving from a polar organic DMSO solution to an aqueous environment, the presence of lower and higher order self-associated SSA aggregates was confirmed using quantitative 1 H NMR spectroscopy in a D2O/ 5% EtOH solution, at a total SSA concentration of 5.56 mM, as shown in Table . Here EtOH was used as the internal standard, and confirmed the presence of higher order aggregates, demonstrating solid like properties for homogenous solutions of 1, 3, 5, 7 and 9. As expected, the proportion of the SSA incorporated into higher order aggregates remained comparable for homogenous solutions of both enantiomers, with no significant difference in the proportion of SSA to be incorporated into the higher order aggregates of 3, 5, 7 and 9. However, when supplied as a 1:1 enantiomeric mixture, although the trend in increased incorporation of 7 + 9 over 3 + 5 into the higher order aggregate structure was retained from previous observations in polar organic solvents, the proportion of SSA incorporated into these higher order structures was approximately 20 % lower for the 1:1 enantiomeric mixture of 3 + 5 over the homogenous solution of those same SSAs. This supports the hypothesis that, as observed within a DMSO-d6 1% DCM solution (Table ), the combination of SSA enantiomers influences SSA self-association events, causing the amino acid R-group to play a greater role in influencing higher order SSA aggregation events. The size and stability of those self-associated structures present were confirmed through a combination of dynamic light scattering (DLS) and zeta potential measurements respectively (Table ). The dH of the higher order species present at 5.56 mM, calculated from intensity distributions, were found to exhibit similar properties to those SSA self-associated species previously published. The aggregated species produced from 3, 5, 7, 9 and their 1:1 enantiomeric mixtures under these experimental conditions exhibited zeta potential values between -66 to -55 mV, confirming the stability of the self-associated species present to be greater than those of 1 at the same molecular concentration. Therefore, the presence of the alkyl/aromatic R-groups within the amino acid appended SSA are confirmed to increase higher order self-associated aggregate stability.