Two control samples were prepared f or the evaluation of Boc deprotection. A negative control was used with the Boc protected samples and incubated into the Rhodamine without f urther treatment. The positive control was prepared af ter a treatment with 50% TFA acid f or one hour, before the same incubation with Rhodamine. The f luorescence was evaluated normalizing a spot with a f ield of view ~ 50 μm to the signal f rom an equivalent glass treated with traditional deprotection in TFA acid.