Initial DLS screening of copolymers was conducted in 384-well plates using the DynaPro DLS Plate Reader III (Wyatt Technology) which uses a wavelength of 830 nm and scattering detector angle of 173°. For this, copolymers were diluted directly from the reaction volume into HBS-EP+ buffer (10 mM HEPES, 150 mM NaCl, 3 mM EDTA, 0.05% P20 at pH 7.6) (VWR) such that solutions contained 1% (v/v) DMSO. Hydrodynamic radius (Rh) and diffusion interaction parameter (kD) of copolymers was quantified by cumulants analysis by using auto-attenuation with 20 acquisitions, 5 s/acquisition, and four concentrations depending on the molecular weight of the copolymer. This screening enabled selection of copolymers, polymer-PEG conjugates, and polymer-peptide conjugates for later SAXS characterization. For this, copolymers and copolymer conjugates had to be lyophilized to allow for proper SAXS buffer matching. DLS data was collected for lyophilized samples in 384-well plates using the same acquisition settings for concentrations of 1, 2, 5, and 10 mg/mL for polymer backbones and 1, 2, and 5 mg/mL for copolymer conjugates. All samples were centrifuged at 10,000 g for 10 min before loading the supernatant into 384-well plates for data collection. A data filter was applied to all data to assess data quality, following the Wyatt Technology default parameters (minimum amplitude = 0, maximum amplitude = 1, and baseline limit = 1 ± 0.01).