Curcumin proved useful as a spectroscopic tool for measuring the hydrophobic encapsulation efficiency of each LLC particle, with a logD value around 4 at pH 7.4 (estimated using ChemAxon). Lipid films were prepared along with curcumin in ethanol, at 5 mol % with respect to lipid concentration. Samples were redissolved in the appropriate amount of anhydrous ethanol and focused using an aqueous Pluronic F-127 solution, adjusting flow rates such that final lipid:polymer ratios were 10:1 as before (FRR 10). Three separate experiments were carried for each composition and compared to controls in which no lipid was present.