The different equations for the determination of turnover number (TON), catalytic cycle (CC) frequency, and number of fragments per molecule of the enzyme (NF/E) were derived. The parameters differ from each other: the TON is the number of times it takes a mass concentration of the enzyme to convert the substrate to product; the speed (or rate) is the TOF. TON can be <, >, and = 1 for polysaccharides or any hydrolyzable polymer. The CC is the totality of all events leading to the formation of the product and its release; the number of CC per unit time is called the CC frequency; the TON per unit time is not equal to the first-order rate constant (kcat). TOF may vary, but kcat is expected to be constant for