The objective of this study was to conduct a thorough sustainability analysis on the synthesis processes of three porous chitosan particles. This analysis aims to evaluate the environmental and economic sustainability linked to these synthesis processes. The indicators employed to quantify these impacts were first normalized to per gram of beads produced from one synthesis cycle. The next level of analysis normalized indicators to percent removal of methylene blue, since water treatment was the primary motivation for this work. The methods for the methylene blue removal experiments are described in the Supplementary Information (SI) Section 1 with the results in Figure . The assessment of sustainability as well as the uncertainty and sensitivity analyses were completed in Python. The scripts and underlying assumptions for this work are openly available on GitHub for implementation. To estimate the costs of the synthesis processes for the three porous chitosan particles, TEA was used. This cost estimation was performed for materials and utilities, including chemicals, supplies, and electricity. The electricity cost was determined based on the energy consumed throughout the synthesis process. Although the overall cost of the general method used for general chitosan bead synthesis remained constant, variations emerged in the costs linked to procedures using different precursors. The environmental impacts for the different chemicals, inputs and energy were estimated by using LCA. Environmental impacts were estimated for acidification potential (kg SO2-Eq), global warming potential (kg CO2 eq), ecotoxicity: freshwater (CTUe), eutrophication potential (kg N-Eq), human toxicity: carcinogenic (CTUh), human toxicity: non-carcinogenic (CTUh), ozone depletion potential (kg CFC-11-Eq), particulate matter formation potential (kg PM2.5-Eq), and maximum incremental reactivity (kg O3-Eq). These impacts for the inputs used in the synthesis of different chitosan particles were taken from the ecoinvent v3.10 database.