The fourteen-component realistic E. coli bacterial membrane is taken from literature both for CG and AA-simulations. To examine the role of cyclopropane rings (CP) in the acyl chain of lipid over single or double bonds, we have prepared additional two modified membrane systems. The model E. coli membrane containing cyclopropane rings is denoted as NM (Native Membrane), in another system we have replaced all the cyclopropane rings with C-C single bonds and named it as MMS (Modified Membrane with Saturated chains), and in the third system we have replaced all the cyclopropane rings with C=C double bond and named it as MMU (Modified Membrane with Unsaturated chains). The naming of the lipids is done based on acyl chains followed by headgroup (Figure of the Supporting Information (SI)).