The construction of a library of glycopolymers with mucin-like densities of glycans was successfully achieved by derivatizing HA with propargyl groups and then attaching azide-functionalized glycans by CuAAC. The resulting HA-g-Lac L , HA-g-Lac H , HA-g-Gb3, HA-g-Le x glycopolymers and unmodified HA were biotinylated at their reducing termini to allow their assembly into glycocalyx models on a SAv-on-SLB surface. An alternative strategy for anchoring His-tagfunctionalized glycopolymers to a (Ni 2+ -NTA)3-presenting SLB achieved a higher density of glycans on the surface as a result of the smaller footprint of the His-tag relative to SAv. However, we note that the (Ni 2+ -NTA)3-presenting SLB can have the disadvantage of glycan-independent binding to proteins with multiple surface histidine residues, and would therefore be incompatible with recombinant lectins having His6 purification tags.