All image analysis was performed on an Xeon W-11855W processor with 32 GB of DDR4 RAM and an RTX A5000 Laptop GPU. Alignment Fourier Transform (version 0d63f0a, ), implemented in MATLAB (Mathworks, v2023b), and OrientationJ were used to analyse synthetic data. AFT used a window overlap of 75% and the indicated window size. OrientationJ used a local window (σ) of 2 pixels using the Cubic Spline Gradient. The function 'Vector Field' was used with a grid size of 10 to sample the Tensor. The Skeletonize scripts used . All source code of RFT can be found, together with instructions, at .