To highlight the new modifications to the ILSA, we present an example analysis using both the original and updated algorithms followed by a possible interpretation of the results. Consider the low-fragmentation is-CID mass spectrum shown in Figure . This spectrum is a DART-MS measurement of a methanol solution containing equal masses of xylazine, acetyl fentanyl and heroin (purchased from Cayman Chemical, Ann Arbor, MI). We collected the spectra using identical instrumentation and method parameters as , including parameter switching to simultaneously collect three is-CID fragmentation spectra (at +30 V, +60 V, and +90 V orifice 1 voltages).