At the same time, diazepam showed low metabolic clearance (CLint = 4.60 μL/min/mg) and an extended half-life (t1/2 = 301.30 min) . In addition to the metabolic stability of the tested compound, the amount of formed 7-OH-THA metabolite was also monitored, as it is a precursor of hepatotoxic quinone methide derivative . Among the THA derivatives, compounds 5i and 5m exhibited high CLint and low t1/2 values, meaning that these compounds are rapidly metabolized, and their bioavailability in vitro will probably be low.