Herein, hydroxyapatite nanoparticles were synthesized greenly using Euclea natalensis roots as a template. Where the synthesis variables, Euclea natalensis, synthesis temperature, and dripping speed on the size and morphology of hydroxyapatite, as well as its potential as a carrier and delivery system for artemisinin in PBS 1X at pH 7.4, were evaluated. This work showed that Euclea natalensis metabolites on the surface of hydroxyapatite nanoparticles improved the artemisinin loading and release.