Taking all these findings together, the KiSSim methodology compares well with established methods while often improving predictions between kinase pairs without an obvious relationship based on the sequence. The pocket sequence and IFP based methods are much faster than the structure-based methods KiSSim and SiteAlign, however, the overall kinase similarity assessment benefits from the added structural pocket information. KiSSim's setup and runtime are more convenient than for the SiteAlign method, however, KiSSim does rely on the KLIFS 85-residue pocket alignment.