We have previously used a combination of fluorescence and transmitted light microscopy to confirm the adhesion and permeation of intrinsically florescent 3 through both Gram-positive and Gram-negative cell membranes, however we have yet to confirm this mechanism of action for live C. albicans. Figure presents the results of complementary studies of 3 with C. albicans (SC5314). Here, C. albicans biofilms were cultivated on a glass coverslip, 3 was added and a live recording of the fluorescence microscopy experiment initiated. Figure show stills collected from this time lapse video included within the supplementary materials. SSA 3 is shown to adhere to the surface of the microbial cell before internalising, demonstrating a preference for the (pseudo)hyphae. These regions are the 'tail-like' protrusions projecting out of the spherical cell body and are involved in pathogenesis. The comparative concentration of SSA present at the cell surface and within C. albicans was also determined with respect to time, Figure . Upon the addition of 3, a measure of background fluorescence intensity was determined (Figure and), any increase in fluorescence intensity, indicating an increased concentration of 3 could then be confirmed. At 180 seconds into the video (360 seconds in real time) (Figure and i) the external surface of the (pseudo)hyphae increases in fluorescence intensity (Figure ), compared to the yeast cell body (Figure ). It takes double the time for the yeast cell body to increase to a comparative fluorescence intensity (360 seconds into the video, Figure ), at which point, 3 has completely permeated the (pseudo)hyphae (Figure ).