3. **Structural Homology and Functional Validation**: The study employed structural homology modeling to compare the T1βOH enzyme with other known enzymes in the paclitaxel pathway, such as T5αOH. TB574 demonstrated structural similarity to known hydroxylases, confirming it as part of the CYP450 family. This helped validate its role as T1βOH, an uncharacterized enzyme in the taxane pathway.