Experimental order parameters can be determined for lipid C-H bonds with NMR techniques such as 2 H NMR ( ) and 1 H- 13 C NMR (10-12) using quadrupolar splitting and dipolar splitting, respectively. These methods are very accurate and highly sensitive to changes in the lipid structural ensemble . There are large amounts of experimental S CH data available in the literature for different lipids measured with both 2 H and 13 C NMR, all in good agreement with each other . Experimental order parameters have been estimated to have at least +/-0.02 accuracy ; the error range of 0.02 is used also in this study, as suggested by Botan et al. , as a sweet spot within which simulated order parameters should ideally reside compared to experimental data.