To report on electrochemistry measured in the detection cell, we need an understanding of the processes which may occur in the reporting cell and how the design of the closed bipolar cell can influence the reporting process. For example, the most commonly used fluorogenic redox molecule to report electrochemistry, Amplex Red (AR), is extremely susceptible to photooxidation. In this process, the fluorescent resorufin is continually produced in any environment with sufficient oxygen and illumination. The accumulation of resorufin results in an increasing background fluorescence during the course of an experiment (Video S0). Furthermore, the rate of photooxidation appears to be greater in solutions which have already undergone some photooxidation (Figures S3 & S4). To limit the impact of photooxidation, some literature sources suggest replacing conventional electrolytes with glucose and sodium hydroxide. Deprotonated glucose, which is generated in the presence of sodium hydroxide, reportedly acts as a chemical reductant, reducing any resorufin produced during photooxidation to dihydroresorufin. It should also be noted that if glucose reduces resorufin produced by photooxidation, it should also reduce resorufin produced by charge passed through the closed bipolar electrochemical cell (i.e. an EC' mechanism with a pseudo first-order rate constant, since glucose is in excess). It has been suggested that this may allow for a direct relationship between the current and the fluorescent signal, as opposed to needing to integrate the fluorescent signal over time. However, the fluorescent response to chronoamperometry induced through a closed bipolar cell, shown in Figure , indicates that the rate constant of the chemical step is not fast enough for a direct relationship between the current and the fluorescence signal. When the working electrode is later held at a reduction potential for the generated hydroxymethyferrocenium cations in the detection cell, the fluorescence observed in the reporting cell dropped exponentially. When the electrode was held at open circuit potential (OCP) after the chronoamperometry, the decline in the fluorescent signal was much slower.