Presently, there is a growing concern about the negative impact and threats to sustainable agriculture resulting for chemical agents such as chemical fertilizer. The continuous use of bio-fertilizers proves to be economical in terms of production, eco-friendly in terms of use, more efficient, productive and attainable to farmers over chemical fertilizers. Bio-fertilizer is favourably the need for present day agriculture, since it is safe and residue free food is increasing on daily basis. In view of the shifting focus towards organic farming and reduction of negative effects and residues in the ecosystem, it is necessary to promote the production of bio-fertilizers in large scale in order to meet the present demand. Bio-fertilizers became popular due to high negative impact of chemical fertilizers .