The first purification step was performed at room temperature via column chromatography (10% MeOH in DCM) using a Sigma-Aldrich silica gel (pore size 60; mesh particle size 220-440; particle size 35-75 µm). The reaction mixture was adsorbed onto silica gel by addition of 1.8 g of silica to the aqueous reaction mixture and evaporation of the solution in vacuo. The resulting powder was stored at -20 °C until loading on a silica column for purification. Collected fractions were analyzed by TLC (10% MeOH in DCM) using UV detection. Fractions containing the 2-Senucleoside were combined and dried under reduced pressure and stored at -20 °C until further purification by semipreparative HPLC.