Comparing the WOP between different optical microscopes, but using similar dyes, samples and field of view, can be done since the WOP is agnostic to differences in absolute signal intensities. WOP values obtained with optical microscopy, however, should not be directly compared with values obtained from analysing MRI data due to the significant differences in spatial resolution (here ~90 μm pixel size in MRI vs ~200 nm resolution in microscopy) and contrast-inducing features.