These non-lamellar phases, particularly the hexagonal and cubic arrangements, are characterized by their highly organized internal structures, which result in distinct physical and chemical properties 147 . The ability of lipids to transition between these phases is crucial for understanding their behavior in biological systems, such as membrane fusion . Non-lamellar lipid phases are important in industrial applications due to their unique structural and functional properties 151 . They can be employed to create stable emulsions and encapsulate bioactive compounds while offering improved stability, with applications in drug delivery and vaccines , functional food products , and cosmetics . Specifically, the application of inverse hexagonal and cubic lipid phases has shown great potential for encapsulation of drugs in e.g. lipid nanoparticles (LNPs) . Figure ,D shows an example of an LNP with the ionizable lipid MC3, built and simulated using the recent parameters from Kjølbye et al . LNPs can be tailored to accommodate specific drugs, making them excellent candidates for advanced drug delivery systems 152, 159-162 . The ability of lipid models to replicate the inverse hexagonal phase under appropriate conditions is a crucial aspect of these models. Specifically, phosphatidylethanolamine (PE) lipids, which are well-characterized in the literature, can form the inverse hexagonal phase at specific temperatures, pressures, and hydration levels . The same is the case for monoolein (MO), a well-characterized and commonly used lipid for creating cubic phase systems . MO, and other monoacylglycerides, can form cubic phases spontaneously at room temperature and pressure . Here, we validate our refined Martini 3 lipid parameters in producing the inverse hexagonal phase of PE and cubic phases formed by MO under various conditions. The formation of the hexagonal phase of PE lipids was tested using two approaches.