The information in Table shows that tau protein has HNH endonuclease, HNH nuclease, HTH CENPB-type, HNH Cas9 enzyme, and HTH La-type RNA-binding domains. The information in Table shows that tau protein has tRNA intron endonuclease catalytic, tRNA-splicing endonuclease subunit Sen15, and tRNase Z endonuclease domains included. The gRNA guides the cas enzyme HNH Cas9. This suggests that telomeric RNA directs the tau protein to the complementary intron region. Subsequently, the HNH Cas9 enzyme eliminates the complementary intron region. This means that the tau protein transposase activity's target region is close to the intron region that matches the guide RNA. This region could be either upstream or downstream of the target intron. This telomeric RNA-guided transposase can stop positioning itself over and over in the same DNA target region by getting rid of the target intron. (4) Endonuclease domains For Extracellular Endonuclease subunit A (IPR020821) to work, it needs divalent ions like magnesium. It cuts DNA and RNA into single-stranded and double-stranded nucleic acids. This residue is located in a conserved region alongside aspartic acid residues, potentially linked to the binding of divalent ions. DDE (IPR032874) A diverse array of transposases, such as those in IS240, IS26, IS6100, and IS26, contain the putative DDE endonuclease domain.