The System Builder module was utilized to integrate the receptor into a 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid membrane surrounded by SPC water molecules in an orthorhombic box, using the OPLS4 force field . The electronic charge of the system was neutralized, and a 0.15 M NaCl concentration was established. Finally, the system underwent equilibration using the default relaxation protocol with Desmond.