The interaction energy between the binding of a single amine and a full pore can be quantified by observing a difference between the single amine binding and average amine binding. In cases where these values are approximately the same, one can suppose that there is little interaction energy. Larger discrepancies indicate an interaction energy that could make a given prediction less favorable in the presence of many amine molecules. Indeed, results in the next sections show that, in the case of the tetramines, the single amine binding energy does not always align with the prediction in the case of a fully loaded pore.