Please do not adjust margins USP5: Improved accessibility Last but not least, an open-source graphical user interface (GUI) named "Auto-VTNA Calculator" was developed using PySimpleGUI (Fig. ). This provides access to the benefits of Auto-VTNA without requiring knowledge of Python coding. Upon uploading kinetic data as an Excel file or multiple csv files, dropdown menus allow the experiments, reaction species for time axis normalisation, and the output species to be selected for VTNA or Auto-VTNA analysis. Calculation and data visualisation features can be adjusted in settings menus, and the results from automatic VTNA can be saved as an Excel or csv file. A "Crop Data" feature can be used to streamline the modification of the input data to investigate the impact on reaction order values, for example, to remove outliers, or to truncate excess data points recorded after reaction completion. By clicking "show + info", the user can also view the overlay score intervals of each reaction species for a selected % from the optimal overlay score (see ESI, Section S8).