Iron oxide is formed by chemically combining iron and oxygen. In nature, iron (III) oxide is found in the form of rust. Generally, iron oxides are prevalent, widely used as they are inexpensive, and play an imperative role in many biological and geological processes. High amounts of bacterial toxicity mechanisms have been observed in metal-based nanoparticles which collectively make it difficult for the development of resistance and result in broadening the array of antibacterial activity. Due to their low toxicity, superparamagnetic properties, such as surface area and volume ratio, and simple separation methodology, magnetic iron oxide (Fe3O4 and Fe2O3) NPs have attracted much attention and found their use in biomedical applications The Pharmacological activity of iron oxide nanoparticles have been illustrated in Fig .