To further investigate the potential toxicity and biosafety of the P-TN-Dox@CM nanogels, the blood was collected from healthy BALB/c mice treated with PBS and P-TN-Dox@CM nanogels after 14 days post intravenous injection to analyze the blood routine and biochemistry index. There was no significant difference between treated group (P-TN-Dox@CM nanogels) and control group (PBS) in terms of complete blood count indexes including white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), and platelet (PLT) (Figure ). Additionally, in comparison with the control group (PBS), liver/kidney functions with the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and serum creatinine (Scr), of mice treated with P-TN-Dox@CM nanogels fell within the normal ranges and no significant differences were found (Figure ), indicating that the P-TN-Dox@CM nanagels did no detectable effects on the liver and kidney functions. As a result, the proposed P-TN-Dox@CM nanoplatform is highly safe and biocompatible in vivo, which could be an ideal candidate for precise chemo-photothermal synergistic therapy of tumors.