The lumen of the multilayers micro-nano tubular scaffold was coated by gelatin, using the perfusion technique to mimic the tunica intima of blood vessels, thereby promoting blood compatibility and endothelialization. Gelatin, known for its favorable properties in these applications, was chosen as the coating material. To prepare the gelatin solution, gelatin was dissolved in distilled water at a controlled temperature of 60 degrees Celsius to achieve the desired consistency and homogeneity. Subsequently, the gelatin solution was loaded into two injection syringes, enabling the precise control of the coating process.