To further confirm the configurations of all the isolated compounds, phloroglucinolysis was performed. Dental Bioassay. Dentin fragments were sectioned into 0.5 × 1.7 × 7.0 mm (H × W × L) pieces and demineralized using 10% phosphoric acid for 5 h. Specimens were treated with the compounds at 0.65% w/v in 20 mM HEPES buffer (pH 7.2) for 1 h, and a control group was kept in HEPES buffer only (n=5). The apparent modulus of elasticity (E) was measured using a 3-point bending method with universal testing machine as previously described. Data were statistically evaluated by one-way ANOVA and Tukey's post hoc tests ( = 0.05).