We have described the combination of TDP data collected with online separation by RPLC and CZE to expand the depth of human proteome coverage. All proteomics methods face the challenge of measuring low-abundance analytes, so identifying robust approaches that introduce new proteoform selectivity are highly sought. RPLC and CZE were shown to possess differential proteoform selectivity that manifests as different physiochemical properties and PTM profiles. In a TDP study of five human tissues, we dramatically expanded the number of proteoforms associated with these tissues by combining the two methods.