The f x value, the inflection point of the titration curve, is guessed by fitting three non-overlapping linear functions to the isotherm. The guessed f x value is then obtained as mean of the intersection of first with the second function and the second with the third function (Fig. ). The heat of formation is calculated using the heat of the third injection Q 2,3 divided by the change in concentration of the added guest [B] component. It is assumed, that at the start of the titration the concentration of the formed complex is nearly the same as the added guest concentration since [B]