Little is known about the scaffold at this point from an off-target perspective. The pyrazolepyrimidine substructure has been recently reported in only one other manuscript to possess antileishmania properties via promoting microtubule polymerization. Moreover, three analogs tested for activity against four human kinases relevant to off-target toxicity displayed moderate-to-no inhibition at a single dose of 10 µM. This would suggest that kinase inhibition may not preclude this scaffold from advancement, however, the kinases tested are not exhaustive and additional experiments are necessary to understand the potential impact the molecules may have on the human kinome. Additionally, even though AC8 maintains significant residual activity at higher concentrations of inhibitor we still do not know if this will be sufficient to alleviate potential learning deficits similar to those observed in the AC1 and AC8 double knockout mice. Further analysis in learning and behavior assays are required to fully investigate any potential impact, or lack thereof, on learning and memory.