Combined with the analysis of the factors affecting the error, this paper believes that the validation method needs to be further improved. In order to avoid the influence of the printer performance and printing ink/paper on the detection, other substances can be considered to replace the printed spots, such as the commercial Polystyrene Microspheres of known size . Since the detection system designed in this project could detect particles at the sub-micron level, the polystyrene microspheres with a size of 1 μm could be detected by the detection system and thus a more accurate measurement error could be calculated. This paper predicts that if the second validation method is adopted, the influence of the printer and printing ink/paper on the detection will be avoided, resulting in the final error much smaller than that of the printing validation method, and further prove the accuracy of the detection system designed in this project is feasible and accurate.