The instrumentation setup consisted of a Vanquish UHPLC system coupled to a Q-Exactive HF Ultra-High-Field Orbitrap mass spectrometer (Thermo Fisher Scientific) equipped with an Ion Max II electrospray ionization (ESI) source. Separation was achieved using a Phenomenex Kinetic Biphenyl (2.1 × 150 mm, 2.6 μm) LC column fitted with a Phenomenex phenyl guard cartridge (4 × 2.0 mm). The sample injection volume was 20 μL (provided by a Vanquish VF split autosampler, held at 4 °C), and samples were eluted using an acetonitrile-water gradient (Table ). The Ion Max II consists of a heat ESI probe (HESI-II) with sheath, auxiliary and sweep gas pressure set at 50, 13 and 3 arbitrary units, respectively. The spray voltage was 3.5 kV. The auxiliary gas heater and capillary temperature were set to 420 °C and 260 °C, respectively.