Silanization in particular, was used in Pečar et al.'s work to bond thermoplastic substrates such as PC, acrylonitrile butadiene styrene (ABS) and PMMA to PDMS microchannels 108 . The oxygen plasma treated thermoplastic substrates were coated with either a 5% 3aminopropyltriethoxysilane (APTES) or a poly [dimethyl siloxane-co-(3-aminopropyl) methyl siloxane (Amine-PDMS). After proper heat treatment, the amine-functionalized thermoplastic substrates together with PDMS were again oxygen plasma treated and brought into contact using methanol as an aligning medium, followed by an additional curing at 80°C for 1 h. Similarly, Sivakumar et al. chemically modified the surface of PDMS using anhydride silane and amino silane reagents resulting in a permanent bond with PET at room temperature via the formation of a stable succinimide group without the requirement of additional pressure to initiate bonding. The hybrid PDMS-PET microfluidic channel can thus be used in high-pressure experiments such as such as the separation of blood and plasma 109 . Bis-[3-(trimethoxysilyl)-propyl]-amine (Bisamino Silane) has also been utilized to bind PC/TPE-hybrid layers to different substrates such as TPE, COC, PS, PC, and glass .