nm. An increase in size was observed in the presence of one and two equivalents of NADH (888.5 nm and 1380 nm, respectively) (Fig. )). However, considerable increase in size was noticed when NADH was added at higher concentration. The size was 1776 nm at 3 equivalent and 5429 nm in size at 30 equivalents of NADH (Fig. ). It is to be mentioned that we preferred not to use PBS due to possible interference of PBS salt during DLS studies. Overall, the DLS study was no detectable species found at 656 nm, which indicated that the aggregated species formed in the presence of only higher concentration of NADH.