In the HRMS method, the focus was primarily on detecting saturated species, however with the use of highly sensitive QTRAP instrument we were able to detect a few additional monounsaturated species. The confirmation of identification of these monounsaturated species was performed by evaluating their retention time behaviour (Figure ). The intensity of these species was much lower compared to their saturated form, nevertheless their detection can provide additional information and contribute to understanding the biological context of the study samples. The targets along with their sMRM parameters have been mentioned in Table .