Considering the modest sample preparation requirements and ability to directly analyze extracts, the wealth of qualitative information achievable from 1D 1 H NMR spectra in tandem with their intrinsic quantitative capabilities via 100% qHNMR evaluation make NMR a highly versatile adulteration assay that combines targeted and untargeted in a single method. The approach is ready for adoption to further applications in DS, food, herbal medicine, and drug analysis. The qHNMR data were acquired at 25°C under quantitative conditions using a 90° single pulse experiment with a relaxation delay of 60 sec, a receiver gain of 46, and 64 scans. Post-acquisition data processing was performed using zero-filling of the 64k FID to 512k data points, a mild Lorentzian-Gaussian window function (Exponential factor -1.8, Gaussian factor 0.9 in GF mode), and baseline correction with a fifth-order polynomial function. The residual DMSO-d5 signal was used for chemical shift referencing (2.5000 ppm). The NMR spectra were exported as a jdx (JCAMP-DX) file for quantitative processing in Mnova. H NMR spectra of the CPC fractions of one of the "curcumin"-containing dietary supplements. CPC was employed as an orthogonal and preseparation method for two reasons: first, to widen the chemical window and dynamic range for the targeted identification of the declared curcuminoids, vitamins, and piperine alkaloids; second, for the untargeted detection of unwanted constituents. The separation was performed as in normal phase (ascending) mode with hexanes-CH2Cl2-MeOH-water (3:7:7:3) as a two-phase solvent system. The components eluted and eventually extruded (EECCC) in order of increasing polarity and were divided into seven fractions (see main text for weight distribution). The vertical scale of the spectra was chosen to show the major components. The intensity scaling factors reflect the vastly different abundance of the various components and highlight the achievable dynamic range enhancement of CCS pre-fractionation. (1). The NMR fingerprints also contained the diagnostic signals of piperine (4; reference spectrum in A), despite its relatively abundance at 0.54% and 2.01% for batch A and B, respectively. In addition, four aromatic impurities were recognized in both CDS batches. Among the impurities compounds, impurity 4 revealed an AMX spin system which may synthetic precursor has the partial structure as the aromatic part of curcumin. For the relative qHNMR analysis, the integrals of the impurities were selected as indicated by the boxes (see Table ).