Phase contrast microscopy images of four fields of view per well were recorded using the ORCA-Flash4.0 V2 camera (Hamamatsu Photonics, Hamamatsu, Japan) and the Plan-Apochromat 20×/0.4 objective with ZEN software. The recording duration was 48 hours, with a 10-minute interval between frames. Time-lapse series were analyzed using Fiji software, and life histories of individual cells were constructed and analyzed as described previously.