was streaked out on LB agar from frozen stock. Liquid culture was created from a single colony isolated from the LB plates and allowed to grow for 6 hours at 37°C in 5 mL of LB media. 100 µL of LB media was added on top of 100 µL of solidified hydrogel of varying concentrations with the presence or absence of vancomycin in a 96-well plate (round bottom, Sarstedt), followed by 5 µL of bacterial culture. The lid of the 96-well plate was coated in anti-fog solution (0.05% TritonX-100, 20% Ethanol in water and allowed to completely dry under the clean bench hood. The closed with lid 96-well plate was placed in the Agilent BioTek Epoch 2