Information of such interactions in both the organic and aqueous phases has been vital in our understanding of the formation of various local processes. For example, if the relevant chromophore of a surfactant exhibits a red shift in a non-polar environment, we may say that this is possibly due to intermolecular H-bonds, thus inferring the presence of dimeric, trimeric or polymeric states that they are in. Higher order shifts would also be possible in a non-polar solvent if the latter contains a small amount of water thus promoting the formation of such a selfassembled structure as an inverted water-in-oil emulsion. When the surfactant is present in aqueous phase, other types of chromic states could also be observed due to the polybasic character of the carboxylic acid , which could be interrogated via pH dependent Bathochromic titration (see below). While the UV spectroscopy allowed us to interrogate various types of self-assembled structures that are formed, these studies, when it is carried out in parallel with a dissipative particle dynamics (DPD) and/or combined with a molecular dynamics (MD) simulation, we gained even a deeper insight into these structures and their stability.