8) Incorporation into the bioactive compound. Finally, we needed to show the utility of aliphatic CF 3 -diazirines for medicinal chemistry projects. Therefore, we incorporated representative CF 3diazirine into a bioactive compound. Previously, sulfonamide 47 was identified as a promising broad-spectrum inhibitor of cancer cell lines (Table ). Here, we replaced the fragment of piperidine with the corresponding CF 3 -diazirine 6g to obtain compound 48 (please, see SI for details). Both compounds were tested for their in vitro antitumor activity against a panel of sixty cancer cell lines (please, see SI). Results against five selected tumor cell lines are summarized in Figure . Original compound 47 inhibited the growth of all five tumor cells K-562, NCI-H522, HCT-15, SW-620, OVCAR-3 (Table ). It's CF 3 -diazirine derivative 48 was also active against three cell lines NCI-H522, HCT-15, SW-620. These results demonstrated that the trifluoromethyl-diazirine fragment was compatible with the bioactive compound, did not significantly affect its bioactivity, and hence could be used in medicinal chemistry projects.