Previously, X-H BDEs for amino acids in proteins have been computed by Moore et al. using the isodesmic reaction method at the B3LYP/6-31G(d) level of theory. This study was an important step forward, as it was the first detailed catalogue of BDEs for proteins. However, while in the past often used, B3LYP is one of the less well performing functionals for radical reactions. Also, the set of references used previously 5 lacks proper reference compounds for benzylic C-H, carboxylic O-H, and aromatic N-H bonds. Instead, tert-butyl amine was used as a reference compound for all side chain N-H bonds.