To control the proton concentration in diff erent solvents, our strategy was to use electrochemically generat ed acid that was monitored by a ref erence f luorescence label. We used a design of an electrochemical reactor previously proposed by us, where the acidity is generated by proton exchange reactions . The platform holds several miniaturized electrochemical reactors each of these consists of three electrochemical partial cells containing the working, counter, and ref erence electrodes (WE, CE and RE, respectively) and separated by diff usion barriers. In the c ell containing the WE, the acidity is regulated by a voltage bias applied respect to the ref erence cell, which controls the release or adsorption of protons by molecules f unctionalized in the WE. The electrodes are isolated f rom the electrolyte using a f luorinated proton permeable membrane (Naf ion) . The diff usion barriers assure that the number of protons in each cell is stable for periods much longer than the dissociation dynamics, while the small dimensions of the cell assure a rapid f eedback of the reactions in the cell. The electrochemically generated acid released protons, f ree of counter ions and f ree to interact with the elements of the electrolyte. Thanks to these characteristics, the acidity can be controlled and estimated by the f aradaic currents. To monitor the proton activity in our cell, we used the f luorescent dye carboxyfluorescein (FAM). FAM allowed a calibration using an external titration that coincided with the proton concentration calculated using the Faradaic currents. We also calibrated the system using a special tautomerization process that FAM undergoes at a particular pH, and we used the proton quantif ication to control the acidity in acetonitrile (ACN). We used the f luorescence to calibrate the proton activity of acids in an organic electrolyte, observing the formation of super-acids due to the inf luence of proton carrier salts in the electrolyte. Finally, we used our electrochemical cell for the deprotection of acid labile groups. In our case we proved the deprotection of ter-Buthyloxycarbonyl, (Boc) groups f rom an APTES monolayer grafted on a glass surf ace upstanding to the electrodes in the cell of the WE. This kind of reactions added a proof of the activity of electrochemically generated acid, which in traditional chemistry is obtained af ter using high acid concentrations.