We assessed the two different constraint topologies of cholesterol on the membrane properties by performing runs with the original Martini 2 cholesterol model and with the optimized geometry of virtual sites. We simulated both models using lincs order = 4, 6, 8 and time steps of ∆t = 10, 20 and 30 fs to check for possible temperature gradients and to evaluate the dependence of structural and dynamic properties on the lincs order and the time step.