The lamellar phase can be observed at temperatures ranging ~273 to 295 K and with ~6 to 11 water molecules per lipid . The transition from stacked DOPE bilayers (Figure ,A) to the inverse hexagonal phase (Figure ,B) was tested for temperatures at 273, 295, 303, and 320 K, and a hydration level of ~3 and 11 water molecules per lipid. The inverse hexagonal phase was observed to form across all the temperatures at low hydration (~3 water molecules per lipid), while for the higher hydration levels, the inverse hexagonal phase was only observed at 320 K, whereas at 273, 295, and 303 K the lamellar phase remained. This aligns with experimental results , except for at 303K, where the inverse hexagonal phase is suggested to form experimentally.