Blood was collected at intervals of 0.25, 0.5, 1, and 3 h (for 1) and 0.5, 1, 3, and 5 h (for 2) post dose from the dorsal metatarsal vein and transferred into plastic micro centrifuge tubes containing anticoagulant of EDTA-K2. Blood samples were centrifuged at 4,000 g for 5 min at 4 °C to obtain plasma. The plasma samples from each time point were pooled and then analyzed by LCMS/MS. The PK parameters were estimated by non-compartmental model using