Multi-conformer partial charge generation methods take multiple conformations as input and attempt to output a single "best" set of partial charges, thereby reducing conformer dependence. OpenEye's Electrostatically Least-interacting Functional groups (ELF10) method works by averaging the partial charges of 10 diverse conformers from the top 2% of a molecule's conformers with the most favorable electrostatic intra-molecular energies after all partial charges are made positive (this approach is designed to exclude conformers with strong internal electrostatic interactions). Here, using this approach (OpenEye AM1-BCC ELF10), we generated input conformers using rdkit v2024.03.2. Conformers were generated starting from 50 di erent random seeds to generate 50 sets of 500 random conformers, the minimum required for OpenEye AM1-BCC ELF10 charges. We then charged each molecule 50 times, one for each set of 500 conformers, with OpenEye AM1-BCC ELF10 partial charge generation method using open -toolkit v0.14.3 and openeye-toolkits v2023.2.3. 40