1% gel solutions were prepared in water and incubated. A stock of Thioflavin T (ThT) was prepared in water. Peptides and Thioflavin T were mixed in 96-well plates (COSTAR Flat plate Black) with final concentrations of peptide of 1 mg/ml and 10 µM of ThT. The fluorescence spectra were measured from 455 to 600 nm at a scan rate of 100 nm/min. The excitation wavelength was 420 nm. All fluorescent spectra were recorded using an Infinite 200 Pro (Tecan) plate reader. Experiments were performed in triplicate.