RNA interference (RNAi) remains a powerful tool for targeted gene silencing, with improved vector designs and delivery methods enhancing its efficiency and specificity . Virus-induced gene silencing (VIGS) offers a rapid method for transient gene silencing, particularly useful for functional genomics studies . Emerging technologies such as optogenetic and chemogenetic tools are enabling spatiotemporal control of gene expression and protein activity in plants, offering new possibilities for metabolic regulation . Nanomaterial-mediated transformation, utilizing nanoparticles for DNA delivery, shows promise in improving transformation efficiency and tissue specificity . The integration of these tools with high-throughput phenotyping and genome-wide association studies is accelerating the pace of metabolic engineering in plants . This expanding toolkit provides researchers with unprecedented precision and flexibility in manipulating plant genomes and metabolism, driving rapid advancements in crop improvement and biotechnology.