The standard deviations are in excellent agreement between the 0.2, 6, and 30 ns runs, illustrating rapid convergence (Figure ). In general, the curves of σ ∂H ∂λ are extremely similar between the vanish stages, but σ ∂H ∂λ is slightly higher for the discharge legs. This may stem from greater rearrangement of the polar solvent environment than of the less polar binding pocket upon discharging. The shapes of the standard deviation curves are ) are computed with respect to equally-spaced λ-windows.