SH-SY5Y neuroblastoma cells (Purchased from NCCS, Pune) were cultured in a humidified atmosphere, containing 5% CO2 in a complete medium composed of Ham's F-12K (Kaighn's) Medium (A modification of Ham's F-12 nutrient Mixture), 100 units/ml penicillin, 100 μg/ml streptomycin and 20% fetal bovine. Every 2 to 3 days the medium has been replaced. Cells were separated by using a 0.125 % trypsin solution and harvested by centrifugation at 1000 rpm for 5 min. Cells were resuspended in 1 ml of medium and viable cells were counted using trypan blue (0.4 % w/v in PBS).