Our previous study demonstrated ML-driven SERS platform exhibits exceptional performance on identifying purified glycans . However, the additional purification process is time-consuming and may result in the loss and degradation of glycans. A new approach allowing direct analysis of unprocessed biological samples and intact glycoproteins will benefit the scientific communities. For the proof-of-concept, we first evaluate the feasibility of analyzing milk oligosaccharides in the unprocessed milk samples.