The application of our AmpliFold format was further evaluated in experiments where HER2 antigen was spiked into a human serum. Although this work aims to report AmpliFold primarily as a proof-of-concept using a model antigen system, these experiments sought to provide some insight into the robustness of AmpliFold chemistries and performance when detecting analyte targets within a complex sample matrix where the additional step of incorporating control lines was performed.