If true exhaustive sampling was achieved, we would expect to find a left-and right-hand version of this single helix. Identifying a single helical cluster does not change the calculated energy-gap Z-score and RM SD inter from the lowest energy cluster to other clusters; however, it does indicate that in some cases these sampling techniques may not be able to accurately capture the other identified clusters. In particular the θ B = 116 • model (figure ) and the θ B = 128 • (figure ) model exhibit this poor sampling.