Currently the organic reactions which can be used for constructing dissipative assemblies are still limited . The exploration of new organic reactions for dissipative assemblies can extend the current research domain of the construction of dissipative assemblies. Thioesters are high-energy compounds and play a crucial role in cellular energy transfer and various biochemical. Recent research indicated that thioesters could have played the role of adenosine triphosphate (ATP) in prebiotic metabolism before phosphor entered the metabolic reaction network . On the other hand, thioester can hydrolyze in aqueous solutions under acid or basic conditions. Thus, it is possible to construct a new thioester formation/hydrolysis reaction cycles for constructing dissipative assemblies. The challenge part is to synthesize thioesters in aqueous solution under a non-enzymatic condition. Through the literature search, we found that thioester can be synthesized in aqueous solution by activating the carboxylate with phosphate anhydride bond followed by automatic thioesterfication . Thus, we assume that T3P, the coupling reagent frequently used in amidation might be able to activate the carboxylate group for thioesterification because when reacting with a carboxylic acid, it forms a similar acetyl ethyl phosphate [20] that can then form a thioester. Thus here we investigated the construction a new type of primitive thioesterificaiton/hydrolysis reaction cycle and further explore to use the reaction to construct non-equilibrium molecular assemblies. This primitive reaction cycle could give some informative clue about how primitive metabolism-like reaction drive the generation of non-equilibrium molecular organization.