This section presents the results of a heuristic simulation study that was designed to further illuminate the behavior of the tight-binding inhibition model and gain a practical experience in handing k obs values derived under tight-binding experimental conditions. The numerical simulations were performed by using the software package DynaFit . All algebraic computations data fitting procedures were also independently verified by using the software package GraphPad Prism version 9.1 (GraphPad Software, San Diego, California USA, www.graphpad.com). The GraphPad Prism files, including the embedded user-defined fitting equations, are made available as part of the Supporting Information attached digitally to this report.