The proportion of frames with occlusion, and the associated uncertainty, were compared between glycoforms for each model (Supplemental Figure ). The Wilson score 95% confidence intervals for each proportion is shown in red and calculated from the occlusion autocorrelation lag times, which were used to estimate the number of independent occlusion configurations sampled. Differences in occlusion propor-tions, particularly between the low occlusion batch and the high occlusion batch, are statistically distinguishable and this finding is true for all 5 model sets. Differences within occlusion batches are not statistically differentiable. To assess the dependence of these results on the starting structural model, for each glycoform we averaged the proportion of frames with occlusion across the five models and compared the means and associated standard deviations (Figure ). Occlusion proportions are consistent across the models for almost all the glycoforms, particularly those in the low and high occlusion batches. There is considerable variability in the glycoforms of the medium occlusion batch (GF 6, GF 11) but considering the consistency in the other models, this might result more from initial glycoform minimization/optimization than the model itself.