The custom MatLab program analyzed each band by first allowing the user to define the boundaries of the lane in which the bands were located. The program then allowed the user to define an upper and a lower bound and the width of the lane that encompassed a particular band of interest. It was important that the program objectively defined the actual boundaries of each band to prevent user error from influencing the obtained intensity values. The user's only influence on the program was to define the rough boundaries of a band in which the program could then search for and define the actual band.