A major obstacle to the TEM study of these wax materials is the high sensitivity of organic materials to the electron beam and their high susceptibility to damage, in particular by radiolysis. Long chain alkanes have previously been reported to undergo significant damage at a cumulative fluence as low as 5-10 e -/Å 2 under 300 keV irradiation, but recent advances in low-dose electron microscopy techniques have enabled improved access to nanoscale resolution in organic molecules. Scanning electron diffraction (SED) has particularly demonstrated suitability for low-dose operation. In SED, a nearly parallel, low convergence angle probe is scanned across a sample at nanometer scale steps while 2D diffraction patterns are simultaneously acquired at every probe position. A simplified schematic of the 4D-STEM technique is presented in Figure . By combining imaging and diffraction data at nanometer spatial resolution (< 5 nm) using low beam currents (~1 pA) and fast detector readout (~1 ms), SED has advanced real and reciprocal space structural analyses of beam sensitive materials, including orientation mapping of semicrystalline polymers, defect mapping in MOFs, and resolving the local crystalline nanostructure in organic molecular crystals. Here we present a combined TEM, AFM, and SED approach for the microscopic analysis of C31H64 and C30H61OH crystals and their binary mixtures. We first identify the characteristics of the pure C31H64 and C30H61OH crystal structures by TEM and AFM. Then, through SED we identify the nanoscale spatial arrangement of chain-ordered lamellae within the pure components and evaluate lamellar structure, lamellar size, and interlamellar disorder. These results outline microstructural models consisting of hierarchical grain structure containing domains with highly ordered lamellar packing. In turn, we discuss the trends in lamellar packing and ordering across a series of binary mixtures. Together, these findings describe the native structural heterogeneity in replica leaf waxes at the nanoscale, providing new insights for models of water vapor and other molecular diffusion through IW layers as well as microstructural principles for the examination of wider wax structure-function relationships.