In conclusion we have shown that a rapid 1 H-15 N HMQC experiment allows for the first time, direct observation of inter-and intramolecular hydrogen bonds to nitrogen in multiple supramolecular architectures including interlocked architectures and hydrogen-bonded dimers, at natural 15 N abundance and readily accessible temperatures. This method should be practical on many spectrometers and can resolve conformational and tautomeric configuration. The experiment is likely to be particularly powerful in deconvoluting complex systems comprising multiple different hydrogen-bonded motifs to resolve speciation. Taken together our data demonstrate the broad utility of this rapid 1 H- N HMQC experiment for potential analyses of an extensive array of supramolecular assemblies involving hydrogen-bonds to nitrogen. Future studies will be directed towards harnessing the experiment for more quantitative analyses of such hydrogen-bonded systems, and developing approaches to other classes of hydrogen-bond e.g.