Brightfield microscopy has been widely used to characterize properties such as flocculation, coalescence, and size distributions of droplets in food emulsions but does lack target specificity to provide additional insights in interfacial structure. Fluorescence-based confocal laser scanning microscopy (CLSM) on the other hand provides good background suppression and some target specificity by using, for example, auto-fluorescence of proteins or dyes that report on the surrounding chemical environment, and was recently used to map lipid and protein oxidation in mayonnaise Despite the progress, finding and applying the right protein-specific marker has remained challenging and the spatial resolution achievable with CLSM of around 200 nm limits our ability to visualize interfacial structure . Electron microscopy techniques provide better spatial resolution, but resolving the droplet interfacial composition within an emulsion like mayonnaise involves cumbersome and invasive sample preparation steps, and only limited progress has been made . Single-molecule localization microscopy (SMLM) allows to overcome the so-called diffraction limit of fluorescence microscopy such as CLSM. The core idea is to switch fluorophores between a fluorescent and non-fluorescent state such that single emitters can be identified and fitted with a 2D Gaussian function to localize the emitter with high precision and accuracy. One particular implementation of SMLM is stochastic optical reconstruction microscopy (STORM), in which the induced blinking of standard fluorescent probes attached to targets of interest allows achieving sub-25 nm resolution . We recently applied STORM to localize fluorescently labelled phosvitin at the interface of turbid oilin-water model emulsions .