Up to now, there have already been numerous studies to learn the effects and mechanism of mutations in the RBD domain . In particular, RBD mutations containing E484K, N501Y and K417N have attracted considerable attention from researchers . Whereas, the details of the NTD mutations have been still elusive until now, owing to the absence of attaching great importance to this antigenic site. When it comes to researches about NTD, recent reports elucidated that several currently circulating variants, comprising Beta (B.1.351) and Alpha (B.1.1.7), harbors some NTD mutations like Y144del, R246I, etc., and these lineages will partially or completely escape neutralization mediated by a variety of mAbs revealed that the RSYLTPGD246-253N insertion mutation in the N-terminal domain of the Lambda S protein, is responsible for evasion from neutralizing antibodies . By comparison, even though A222V possesses a high mutation rate according to statistic by GISAID sequence database , it makes no difference to immunogenicity .