Another example of the application of Dy-FMOPhore is the PI3K system, (Figure ). The results highlight that in the static scanning of the binding site the Trp-A-812 was in a closed conformation and makes the orientation of the indole ring not in favorable 3Dgeometry to form a Pi-Pi interaction with the probes. By applying Dy-FMOPhore, the ensemble of the simulation allowed the rotation of the side chain that led to opening of the indole ring. This is shown on the FP-score diagram, where the Trp-A-812 was categorized in the Non-hotspot category and shifted to the Accessible-category using the Dy-FMOPhore.