Other essential cores for this activity are a carbonate chain of twelve members and a lactonic ring, the carbonate chain has an excellent mean activity for this target, but the lactonic ring does not; this is because the lactonic ring has multiple compounds with a harmful activity while carbonate chain has a significant number of compounds with functional activity. PqsR and RhlR DS could not be analyzed with this method because the rules of fragmentation used, does not involve carbonate chains or rings .