Our observations reveal a rich array of morphological and topological transitions driven by the kinetics of adsorption of MO-LCNPs into the lipid bilayer and equilibration processes within the GUV once seeded with excess area and enhanced stored curvature stress in the membrane. The new insights and understanding we have presented in this study are not achievable in previous work on planar bilayer membranes, where the membrane dynamics are suppressed by a solid support, or in living cells, where deep mechanistic insight is frustrated by the complexity of living matter. Future exploration of the broad parameter space of the structure and composition of LCNPs and GUV membranes will promote optimisation of drug delivery and artificial cell technologies.