To gain further insight into the mechanism of yeast-assisted exfoliation of graphite as a function of time using fully atomistic Molecular Dynamics (MD) simulations. Here, we discussed the role of thickness in exfoliating graphene sheets. To this end, we initially considered two layers of graphite (area = 30 Å x 25Å) without any defect which were confined between two cell membranes of the yeast cell, modelled as a phospholipid bilayer made of phosphatidylcholine (POPC) molecules (see methodology and supplementary section for details) at t=0 ps, as shown in Fig. . Within t=16 ps of simulation time (Fig. and)),