We performed similar experiments using SSAs 2 and 3, and found that each of these molecules was able to mediate a small amount of chloride efflux independently of the addition of anionophore 4 (Figures ) and 2 (d)). However, the total chloride efflux observed when adding these SSAs in combination with anionophore 4 was not significantly larger than the sum of the efflux mediated by the SSA and 4 independently. We hypothesise, that due to the amphiphilic nature of SSAs, that these observations maybe attributed to the SSA surfactant which result in small amounts of vesicle lysis at higher concentrations (see also Section S4).