The analysis of TBARS (thiobarbituric acid reactive substances) was performed as described by Steppeler et al. . The assay is based on the formation of a stable chromophore through the binding of aldehydes like malondialdehyde (MDA) to thiobarbituric acid (TBA) under acidic conditions and heat. In order to precipitate the proteins and stop the enzymatic activity, trichloroacetic acid (TCA), propyl gallate, and EDTA was added to the samples. MDA equivalents (µmol/kg sample) were quantified as the difference in absorbance between 532 and 560 nm (SPECTROstar Nano, BMG LABTECH, Ortenberg, Germany). TBARS analyses were performed in triplicate.