Among the pressures tested, the range of 8.17 to 32.67 kPa produced the highest SNRs, with no significant differences in SNR across this range (Figure ). Meanwhile, the %CV values were lowest when 32.67 kPa was applied, at intra-assay %CVs of 6.76% and 2.47%, as well as interassay %CVs of 4.99%. This shows that the value of 32.67 kPa was most repeatable and reproducible for industrial applications. Although 16.33 kPa resulted in intra-assay %CVs of 5.69% and 10.05%, and inter-assay %CVs of 8.91%, which are within acceptable industrial ranges for repeatability and reproducibility, 32.67 kPa demonstrated greater consistency overall, with lower %CVs. (Table ). Additionally, among all the fluorescence images in Figure , 32.67 kPa produced the most uniform and complete squares. Exceeding 32.67 kPa resulted in a significant reduction in SNR. At these high pressures, stamps also experienced more buckling, higher damage to the PDMS features and higher occurrences of double printing. Additionally, the inter-assay values begin to increase and exceed the industrial threshold, reaching 10.48% and 27.65% for 40.82 kPa and 49 kPa, respectively (Table ). While all squares were successfully printed, buckling of the stamp features caused visible holes in the fluorescence images in the fluorescence images, resulting in nonuniform fluorescence and increased inconsistency (Figure ).