The composition of the nanoparticle protein corona is dynamic, complex, and directly dependent on the makeup of the biological media to which the nanoparticle is introduced. The adsorption and desorption rate for each protein on the nanoparticle surface will dictate whether the protein remains as a component of the irreversible corona layer (hard corona) or adsorbs and desorbs (soft corona) in equilibrium with its surrounding environment . Many nanomedicines are intended for intravenous administration, which will subject them to the forces associated with blood flow rates within the circulatory system, as well as biomolecules and cells contained within blood. Therefore, understanding the role of hemodynamic parameters as a function of nanoparticle circulation time and techniques used to recover nanoparticles for downstream analysis of protein composition, and their implications for nanoparticle-protein interactions and subsequent cellular effects is an important aspect requiring consideration in the early assessment of nanoparticle biological performance.