To test this, full-length AAV2 VP analysis was performed using a UHPLC-MS platform involving HILIC with 0.1% DFA added to the mobile phases for LC separation and in-line fluorescence (FLR) and MS detection for VP characterization (discussed in Section 3.3). Separations were performed at 25°C, 45°C, and 60°C to observe the impact of column temperature on VP separation, with triplicate injections performed at each temperature. As demonstrated in Figure , separation of the three VPs was achieved at all temperatures tested (Figure ) and high-quality MS spectra was obtained for each VP peak (Figure ). VP elution was found to be consistent with previous VP separation on HILIC ,