Alternatively, a high temperature of 100 °C can be applied to achieve the same outcome. However, both ways are not suitable for POC applications because of the need for personal protective equipment (PPE), heating equipment, and a way for disposal of used chemicals. Importantly, reusing a biosensor requires detailed studies on their stability after heat or NaOH treatment conditions. Clearly, the use of nucleic acid-based sensors for continuous monitoring in, for instance water treatment facilities, is now limited.