AlphaFold 3 predicted the protein structure of clusterin protein . The models were acquired, and their structural accuracy was confirmed. The Vfold2D and Vfold3D servers received the aptamer sequences and produced 2D and 3D structural predictions, respectively . The stability and possible binding conformations of the secondary and tertiary structures were examined. Using molecular docking software, the 3D structures of the aptamers were positioned onto the anticipated protein structures. The binding locations and interaction strengths were determined by analyzing the docking results. Chimera software was used to visualize the individual structures and the docked complexes (23) . For additional investigation, figures showing the structural alignments and interactions were created. An aptamer binding site on the proteins was predicted using a graph neural network (GNN). Known aptamer-protein interactions were used to train and validate the GNN model. The protein sequences were subjected to phylogenetic analysis using MEGA software . Phylogenetic trees were generated to determine links and evaluate the conservation of the aptamer binding sites.