We have thus far described fits to unnormalized data that are direct experimental observations. However, it is a common practice in the analysis of the CRC experiments to normalize the data prior fitting the Hill equation model. Normalization helps "accommodate minor changes to assay protocols to ensure robustness", a key goal of the AGM. ? Minor changes in the assay protocols could include variations in the amount of time between the initiation of enzymatic activity and measurement of the product concentration. Here we address the question of how normalization affects the MLE estimators θc and θctb .