Pseudomonas putida is a cellular organism that can live in wide range of habitats. The natural habitat for microorganisms can be harsh and unforgiving. Through natural selection and evolution these bacteria have adopted different mechanism to survive in harsh or stressful conditions. One mechanism that we have discussed is the cis-trans isomerization of lipids in the membrane bilayer. This cis-trans isomerization is hypothesized to change the fluidity of the membrane to protect the membrane for denaturation under stress condition. We quantified fluidity of the membrane in terms of different physical properties like membrane thickness, lipid order parameter, diffusion coefficient, and lateral pressure profile.