The two-layers separation suggests that graphite-yeast interaction to be sufficiently strong to completely overcome the inter-graphite sheets Van der Waals (VdW) interactions. Similarly, we also considered six parallel graphite layers (area = 40 Å x 70 Å) shown in Fig. (d), to determine whether the layer separation could also occur for thicker structures. As observed for two-layer case, within t=16 ps of simulation time, layers started to separate, showing again that the graphite/yeast interactions are strong enough to overcome the Van der Waals forces of thicker layers. This phenomenon was supported by Fig. and), the concentration profile of the graphene corresponding to the [0 0 1] direction shows that the confined fluid has a higher density closer to the membrane surfaces indicating the sticking to be mediated by shear stress. The yeast sticking phenomena of six layer is slightly higher in comparison to two-layers, this can be attributed to the higher surface area. In summary, the MD results further support that the yeast-membrane interaction with graphite are sufficiently strong to wet its surface and induce exfoliation by shear stress, even considering the thicker graphite structures (further details given in the supplementary file).