Binding Mode Rationale via S/TEM Imaging. Electron microscopy was further employed to capture "snapshot" images of the QR-lectin complexes in order to provide a more detailed understanding of their interactions. Here, QR-lectin samples, prepared with a PQR of 4:1, were plungefrozen and then vacuum dried, before being placed for S/TEM imaging. We have shown previously that this method allows for the successful capture of the native dispersion state of nanoparticle assemblies. Binding of DC-SIGN demonstrated mostly isolated individual QRs (Figure ) which correlated nicely with the single 𝐷 h distribution for DC-SIGN•QR-DiMan complexes observed by DLS (Figure ). Additionally, binding of DC-SIGNR yielded both clustered QR assemblies and non-clustered individual QRs (Figure ), which again agreed well with the two distinct 𝐷 h species observed in DLS (Figure ).