The selectivity of SOµC-Bdpy1 and SOµC-Bdpy2 for GSH detection respect thiol containing cysteine (Cys) and Homocysteine (Hcy), possessing distinct roles in cellular function and metabolism, was also examined. For this purpose, the microchips were incubated in 1 mM solutions of either Cys or Hcy overnight. Figure exhibits a negligible change of fluorescence intensity indicating the high selectivity exhibited by SOµC-Bdpy1 for GSH detection. Figure also shows comparable results for a similar experiments using SOµC-Bdpy2. These results manifest an high selectivity of SOµC-Bdpy1 and SOµC-Bdpy2 for the GSH detection, especially taking into consideration the much higher intracellular concentration of GSH compared to the Cys and Hcy . SOµC-Bdpy1 and SOµC-Bdpy2 exhibited comparable fluorescent intensity when they are incubated in PBS alone or cell culture medium during overnight incubation time indicating that there is no negative effect on the application SOµC-Bdpy1 and SOµC-Bdpy2 in the presence of cell culture medium (Figure ).