These isothermal amplification techniques were incorporated in the development of biosensors and state-of-art devices, showing great progress in POC devices development. For instance, Ben Aissa et al. ( ) have reported biotin-streptavidin based approach. Briefly, the target DNA was hybridised with a biotinylated capture probe allowing its attachment to streptavidin-modified magnetic beads. Subsequently, the RCA was undertaken to amplify the target DNA strand and the product of the RCA reaction was labelled with HRP enzyme. The biosensor was constructed onto a SPCE, and the used method was SWV. The enzymatic detection was studied in the presence of hydroquinone as a mediator and H2O2 as a substrate. The biosensor achieved a LOD of 6.7x10 -13 M which corresponded to 10 4.3 CFU/mL in approximately two and a half hours. have applied several amplification strategies to improve the sensitivity of the developed STEC O157:H7 detection method. First, they used the 3D DNA walked strategy followed by RCA to amplify the target DNA and hybridization chain reaction (HCR) to immobilise electrochemical indicators (1, 1′-dicarboxyferrocene (Fc) and doxorubicin (Dox)) to the amplicons allowing for an enhanced electrochemical signal, see Figure . (A). As a result, the biosensor exhibited a very high sensitivity with an LOD of 7 CFU/mL in approximately 2.5 hours including the DNA extraction.