Most of the Tecovirimat-resistant mutations mapped between F13L ~ 200 to 300 amino acid residues, before the near-canonical phospholipase-like motif (Figure , green and red circles). In contrast, both E353K (unrelated to Tecovirimatresistance) and I372N, mapped after the phospholipase motif. The phospholipase gap of mutations (Figure yellow vertical line), could be explained for the need to preserve phospholipase activity for poxviral survival and/or replication.