Two decades ago, there were no commercially available QC materials bespoke for LC-MS/MSbased proteomics, though QC1 single-protein (e.g., bovine serum albumin) tryptic digests were commonly used. In part to address this lacuna, the Proteomics Standards Research Group (sPRG) of the Association of Biomolecular Resource Facilities (ABRF) developed two multi-protein standard mixtures, UPS1 and UPS2, in collaboration with Sigma-Aldrich in 2006 . These standards are still commercially available in 2023. The sPRG has also developed a multi-species material that is available through JPT Peptide Technologies and a phosphoproteomics standard that is commercialized by Thermo Fisher Scientific, and a multi-species whole proteome mixture currently in development. These and other QC materials have different applications that in principle can be combined to provide a richer quality evaluation. Though laboratory-specific QC materials are still used, there is added value in using commercial materials continuously available to all labs regardless of instrumentation and geographic location. Broadly, QC materials fall into one or two of the categories defined above (QC1, QC2, QC3, and QC4) and will be discussed here in terms of increasing complexity (Table ).