However, even with readily built structures, the simulation timescales currently accessible with supercomputers may not be sufficient for observing full interdigitation due to possibly existing kinetic factors. A possible future solution to the issue of obtaining self-assembled structures below T m could be a multiscale approach similar to that utilized by Shamaprasad et al. (2022) to study lipids of the stratum corneum. In addition, achieving a gel phase is particularly difficult with the CHARMM36 force field without considerable finite-size effect, and the phase behavior of lipid membranes at low temperatures has been shown to depend on the thermal history and the conditions they are prepared under. These limitations underline the need for further experimental validation to confirm the results on tail order parameters and tilting. Since membrane interdigitation involves a complex balance of forces, it should be acknowledged that other factors may also influence the interdigitation of MMG analogs.