The equal amount (15ml) of aqueous phase (SA+ water) and organic Phase (Extractant + diluent) were charged into a 100 ml Erlenmeyer flask. For efficient extraction, magnetic stirrer was used for phase mixing. The mixing of two immiscible phases at constant temperature were carried out by stirring with a magnetic bar at 1000 rpm for 2 hr. After each extraction batches centrifugation was followed for the clear separation of two phases. The mixed phases were centrifuged at 5000 rpm for 15 min. The two clear phases of organic and aqueous phases were obtained in the centrifuge tube and these two phases were separated by using micropipette. Samples of each phase were taken for the analysis for the concentrations of succinic acid.