The performance of PubChemLite is monitored weekly with every build using the evaluation dataset of 977 compounds established in the original publication . The ranking performance has been quite stable over the three-year period, with median rank=1 of 794 (81.3%), 1-2 of 917 (93.9%), 1-5 of 960 (98. Overall, despite PubChem increasing in content dramatically over that time, PubChemLite has remained generally stable at ~380,000 entries, growing slowly over time. The systematic increase of the BioPathway category (purple, Figure ) starting in October 2022 introduced a number of irrelevant candidates in preliminary results of non-target studies and caused continual expansion; this content was switched to the "Pathways" subcategory rather than the entire "Interactions and Pathways" heading in May 2023 (see Figure ), improving performance and interpretability of candidate hits. The dramatic increase in FoodRelated information was due to the integration of FooDB 41 into PubChem annotation content; despite the large increase in that category, the overall candidate numbers remained stable, indicating that many of these candidates already had other annotation content in PubChemLite. The increase and then decrease of content in the DiseaseDisorder category in March-April 2024 was due to an update of one data source that suddenly introduced many low-quality candidates -upon contacting the contributor they checked their data and identified several issues; the fixes were processed by 12 April (see Figure ). Overall, the continuous monitoring and use of PubChemLite in various NTS studies helps ensure relevance and usefulness for the community.