To quantify the heterogeneity of the experimental data, we first plotted the RPD histograms for aggregation probabilities  of 0, 0.6, and 0.95 (Figure ). We have shown earlier (Figure ) that the relative amplitude of the first maximum increases while the amplitude of the second peak decreases with increasing aggregation probability. To obtain a more quantitative readout, we defined a relative peak amplitude Arel as the first peak amplitude divided by the sum of the amplitudes of the first and the second peak. We obtained relative peaks amplitude Arel of 0.29, 0.49, and 0.93 and localization precision amplitudes (ALP) of 1.73, 6.75, and 12.18, respectively. We then determined the amplitudes of the local maxima e for the simulated data sets with aggregation probabilities  from 0 to 0.99 from Figure and determined the amplitudes of the local maxima (Figure ). The resulting graph shows Arel being constant around 0.25 for an aggregation probability 0 <  < 0.3. For 0.3 <=  < 0.6, Arel slightly increases to around 0.4. For  > 0.6 Arel increases, ultimately approaching unity. Moreover, when fitting over the RPD histograms, the green triangled line representing the amplitude of localization precision (ALP) variation of the RPD histograms show a similar trend as the relative peak amplitude (Supplementary Table ).