(Fig. ). The equilibrium mass density for NAv/BPT ratio to increase as function of tether length for both mushroom and brush conformations, while this increase was found be distinctly higher in case of the mushroom conformation. (Fig. ) Such increase in NAv/BPT ratios indicates that it is possible to obtain a greater density of NAv, for the same number of BPT receptors, just by increasing the length of the tethers. Previous instances in literature have demonstrated that reducing tether density would benefit the adsorption of the ligand owing to a reduced analyte-analyte hinderance. The BPT in mushroom conformation is expected to present a lower steric hindrance for NAv adsorption, which could explain the higher observed NAv/BPT ratios for the mushroom (black curve) as compared with the brush conformation (red curve) as seen in min for increasing tether lengths of 3.2 nm (APT-400), 4.5 nm (APT-600), 7.8 nm (APT-1k) and 79.5 nm (APT-10k). (Fig. )