The APL was calculated using two methods: 1) by multiplying the box dimensions in the xand y-directions with each other and dividing the result by the number of lipids in one leaflet, and 2) through 2D Voronoi tessellation. Using the latter method allows the determination of individual APL values for each lipid, whereas the former gives only the average APL for the whole membrane with the assumption that the membrane is flat. Membrane thickness values were obtained from the average membrane electron density profile peak values along the z-axis with the assumption that the membrane is planar and its surface is perpendicular to the z axis. The thickness of the individual bilayers was also determined for the double bilayer configurations; the distance considered in this case extended from the outer leaflet peak to the electron density peak at the center of the membrane.