Half-life times (t1/2) of soluble HheG D114C and D114C CLECs at 22 °C were obtained based on the determination of deactivation rate constants (kd). Deactivation rate constants at 30, 32 and 34 °C for soluble enzyme, and 36, 38, 40 °C for CLECs were determined by incubating the enzyme preparations at respective temperatures (T). After 0, 15, 30, 60, 90, 120, 180 and 240 min of incubation, samples were taken for reactions containing 50 µg soluble enzyme or 100 µg CLECs, 20 mM cyclohexene oxide adjustments with Coot . Alternating rounds of manual optimization and phenix.refine were used for further refinement. Data collection and refinement statistics are listed in Table .