The analysis of standards yielded 119 files in .raw, of which 56 were from method 1 and 63 from methods 2 and 3. The files were converted to centroided .mzML format using MSconvert 36 (version 3.0.23056) for the method 1 files, while ThermoRawFileParser 37 version 1.4.2 was used for the Orbitrap IDx files (i.e. methods 2 and 3). This last conversion allowed to remove the fluoranthene calibration masses. Subsequently, the MS/MS spectrum for each standard was manually selected using MZmine3 (version 3.7.0) , focusing on the peak apex identified through the extracted ion chromatogram (EIC) with a 3 ppm mass error relative to the theoretical [M-H] precursor. When a MS/MS was extracted from a mixture of standards, the identity of each isobaric feature was confirmed by the relative retention time and specific MS/MS fragments published in our previous works 2 or in the mixture datasheet for commercial mixtures. Then, the MS/MS spectra were converted to .mgf format resulting in 195 different spectra of which 131 are unique compounds. This .mgf files were submitted to GNPS libraries employing the 'Batch Upload of Annotated Spectra' workflow (). Additionally, another submission was done employing 217 MS/MS .mgf files of commercial standards obtained from Watrous et al. study focused on enzymatic oxylipins . This building procedure was summarized in Figure .