vector is parallel to the membrane surface. Comparing the distribution of protein poses (Figure ), the wild type protein spends more time occupying a narrow range of poses, while the double mutant occupies a wider array of poses across its simulations, and does not show as strong of a preference to any pose. In the wild type, the most favorable position was found at 42°tilt (Figure right side), which closely resembles the binding position previously described, while the mutant's most favorable position is found at 32°tilt. This shows that the W238AF241A mutant results in a decreased stability of pose specific to the binding area of the protein, supporting the hypothesis that, although the double mutant does not result in dissociation of h-cisPT from the plasma membrane, it may be prevent the formation of a stable binding pose necessary for ligand insertion.