The preceding results (Figure and Table ) establish the viability of RuAAC as a qualitative derivatization tool for naturally occurring internal alkynes, even in complex matrices such as crude carrot extract. The technique is effective in all of the solvents tested, and maximal derivatization occurs after 6-10 hours at room temperature. No special anoxic or anhydrous techniques are required to observe significant signal increases. The data also showed that primary alkyl azides (2b and 2c) both outperformed aryl azide (2a) as a derivatization agent. Ethyl azidoacetate (2b) was particularly effective, as both the absolute intensity of the doubly-derivatized falcarindiol (7b) and the ratio of 7b to mono-derivatized falcarindiol (6b) was higher for derivatizations with 2b compared to other azides (2a and 2c). For these