-3,4,9,10-tetracarboxylic diimides (PDIs) are the class of highly admired organic dyes and pigments with far-reaching applications in the fields of academia and industry. PDIs with their extraordinary structural, photophysical and redox properties have been exhaustively investigated by researchers as non-fullerene photovoltaics , organic electrode materials , photocatalysts , and fluorescent probes. Despite of all, the polyaromatic π-conjugated structure of PDIs inclined towards significant aggregation in aqueous solutions leads to fluorescence quenching which restricts its suitability in the domain of molecular recognition. On the other hand, the perylene tetra-(alkoxycarbonyl) (PTAC) as structural counterparts of PDIs with four carboxylic ester chains attached to the perylene core provide the advantages of better solubility, high fluorescence quantum yields, excellent thermal and photochemical stabilities, ease of synthetic and functional modifications which provides high tunable photophysical properties in the organic solvents. Therefore, recently PTAC gained much attention from the researchers and but there are very few reports available in the literature for the development of PTACbased fluorescent probes. Herein, in the present work a novel simply designed perylene -3,4,9,10- to the electron-deficient perylene core (acceptor). We successfully investigated the photophysical mechanisms that participated in the complexation of PTE-EA with Cu 2+ in HEPE buffer-CH3CN (3:7, v/v, pH 7.3) by using spectroscopic techniques.