Then, CD spectra of RBT and SBT in the THF-H 2 O mixture were further studied to evaluate the impacts of aggregates formation on their chiral-optical properties. As shown in Figure and Figure , although the CD band had slightly shifted parallel with the aggregates formation, the CD intensity and sign was nearly independent on the formation of nano-aggregates. This was different from the aggregation-induced CD annihilation phenomenon observed for chiral small molecules and polymers derived from BINOL moieties. For most derivatives of BINOL, the changing dihedral angle between two naphthyl rings would contribute to the annihilation of CD signals after aggregates formation. This remarkable distinction had strongly supported the shape-persistent skeleton of these CCMs, in which the dihedral angles within BINOL were highly constrained owing to the conjugated macrocyclic skeleton.