A calculation script based on MATLAB has been developed to assess the efficacy of the evaluation process. To validate the precision of the ANN predictions, five observation times were selected, distributed throughout the experiment at times 𝑇 : hours. These observation times are consistent with the experimental timeline. The range of the input 𝐷 is the same as in the training datasets from 5.5 × 10 to 3.55 × 10 [𝑚 /𝑠] with a very small interval of 1 × 10 . The precision was chosen to be very high to fully discover the solution space, which is necessary for testing the optimization algorithms presented in the following section. This precision led to 30,000 total nMSE calculations, corresponding to 150,000 simulations that generated five concentration profiles for each input 𝐷, completed in just 19.75 minutes with the results shown in Figure .