Hot embossing is a common technique for the mass production of thermoplastic polymers with relatively low costs . In hot embossing, the polymer plate is heated above the glass transition temperature while it is pressed against a master mold with channel protrusions by a hydraulic press to form the microfluidic arrays as cavities in the polymer. The thermoplastic polymer plates used in hot embossing could be fabricated by injection molding . Before performing the hot embossing process, the polymer plate may be annealed to reduce residual stress . Depending on the polymer type, thickness, polymer chain orientation, and the experimental design, the applied pressure and temperature vary. After the process, which takes a few minutes (e.g. 2-20 min), the pressure is usually maintained as the samples cools down to enhance the uniformity .