The workflow for autonomous MicroED data collection and processing involves several steps. First, a low-magnification atlas is used to screen the grid containing a mixture of compounds, and suitable grid squares are selected for medium-magnification montages. Crystals are then chosen and added to the list for data collection. Next, continuous rotation MicroED movie and an image of the crystal are collected autonomously using SerialEM. The data is then processed automatically in real time. Crystal volume is estimated for compositional analysis. This workflow allows for efficient and autonomous MicroED data collection and processing of thousands of data sets per night, reducing the need for human intervention and saving researchers' time while producing high-quality data. The bars represent the number of crystals identified for each composition in mixture A.