LA-EG4-DiMan ligand-capped GNPs (Gx-DiMan, x = 13 or 27) were prepared by treating the citratestabilised GNPs with LA-EG4-DiMan in an aqueous solution. Briefly, GNPs were incubated with the LA-EG4-DiMan ligand under a fixed GNP: ligand molar ratio of 1:3000 for G13 or 1:10000 for G27. The resulting solutions were stirred at room temperature (RT) in darkness (wrapped with foil) overnight to complete the GNP glycosylation via self-assembly. Any unbound free ligands were removed by centrifugation followed by washing with pure water. The successful preparation of Gx-DiMan was supported by greatly improved resistance against salt-induced aggregation (citrate-stabilised GNPs readily aggregate and produce colour changes upon the addition of NaCl salt which can effectively screen their electrostatic repulsions). Moreover, an increase of Dh by a few nm was also observed for both G13 (from ∼16 to ∼22 nm) and G27 (from ∼29 to ∼32 nm) after glycosylation. This result was consistent with what was expected for G13 or G27 coated with a self-assembled monolayer of LA-EG4-DiMan molecules. The Gx-DiMan solutions were found to be highly stable, and no changes in physical appearance or precipitation were observed after storage at 4 ˚C for more than one year.