Figure shows a schematic diagram of a glass bottle containing the homeopathic potency. The spectra of the solution have been taken from two different portions of the glass bottle (upper and lower part), as shown in Figure . Figure shows the variation of UV-Vis absorption spectra of the top and bottom part solution with different potencies. The presence of three absorption-band is observed for all samples. To know the exact positions of the bands, we have plotted the second derivative of a spectrum as a function of wavelength (Figure ). The occurrence of absorption band at ~557 nm and ~721 nm due to the presence of anisotropic structure of different sized Au nanoparticles with localized surface plasmon resonance (LSPR). Additionally, it also shows that the shape and absorption intensity of the spectra change with potencies. At higher potency (i.e., concentration-10 6 to 10 17 times dilution), a broad absorption band has been observed, and it gradually became narrow/sharp after 24C. Similar variation has been observed for both the upper and lower part of the potencies.