Virus Inhibition. The inhibition effects of Gx-psDiMan (50% and 100%) on 293T cell entry of particles pseudotyped with the Ebola virus glycoprotein (EBOVpp) were assessed using our established procedures. Briefly, 293T cells seeded in 96well plates were transfected with plasmids encoding DC-SIGN or control transfected with empty plasmid (pcDNA). The cells were washed at 16 h post transfection and further cultivated at 37 °C, 5% CO2 in Dulbecco's modified eagle medium (DMEM) containing 10% fetal bovine serum (FBS). At 48 h post transfection, the cells were exposed to twice the final concentration of Gx-psDiMan inhibitor in OptiMEM-medium for 30 min in a total volume of 50 μL. Thereafter, the resulting cells were inoculated with 50 μL of preparations of vesicular stomatitis virus (VSV) vector particles encoding the luciferase gene and bearing either EBOV-GP (which can use DC-SIGN/R for augmentation of host cell entry) or the VSV glycoprotein (VSV-G), which cannot use DC-SIGN for augmentation of host cell entry). Under these conditions, binding of Gx-psDiMan particles to 293T cell surface DC-SIGN receptors can block EBOV-GP interactions with these lectin receptors, resulting in reduced transduction efficiency of the virus particles and hence reducing the cellular luciferase activity. At 16-20 h post infection luciferase activities in cell lysates were determined using a commercially available kit (PJK), following the manufacturer's instructions, as described in our previous publication. ASSOCIATED CONTENT Supporting Information.