All experiments were carried out under the authority of a licence granted by the UK Home Office under the Animals (Scientific Procedures) Act 1986. Prior to submission to the Home Office, the application for the project licence was approved (approval number WEC2024-19) by the University Welfare and Ethical Use of Animals Committee, acting in its capacity as an Animal Welfare and Ethical Review Body as required under the Act. Fifteen female mice of the Balb/c strain were obtained from Charles River Laboratories, UK. Animals were maintained under a 12-hour light / 12-hour dark cycle, in Thoren mouse caging containing EC0 aspen bedding (DBM, UK), with "Sizzlenest" and "Rodent Rolls" nesting material (DBM). Enrichment materials included aspen chew sticks, cardboard houses, tunnels, swings, and sunflower seeds. All animals had ad libitum access to food (5LF2 Extruded Diet, IPS Ltd, UK) and tap water throughout.