Structure is known to be more conserved than sequence, and previous studies have shown that including structural information adds orthogonal information to shed light on unexpected similarities between kinases and off-target effects. To help detect such relationships between more distantly related kinases, we generated KiSSim kinome trees based on the DFG-in conformations, as described in detail in the KiSSim tree section, to investigate all-against-all relationships between kinases compared to the sequence-based kinome tree by Manning et al. . Note that we can base the comparison on structurally resolved kinases only, i.e., 257 out of the roughly 500 human kinases.