Actively growing bacterial stock culture in "Mueller Hinton Broth" having around 5.5×10 7 living cells were taken (separately for both bacteria species). The simultaneous release of DT from the micelle (by the method described in section 2.7) and the reduction of viable bacterial cell count were observed for both Brij systems. For solid state culture the assessment was performed through inhibition zone measurement using two model bacterial species grampositive Bacillus subtilis and gram-negative Escherichia coli . The bacterial stock culture was prepared in a low pH (1.5-2.0) and human body temperature (37 ℃) system so that they can withstand the DT release medium having acidic pH resembling simulated gastric environment which was used to execute the DT's release.