Human induced-pluripotent stem cells (hiPSC; IMR90-4, WiCell Research Institute, USA) were cultured in mTeSR™1 medium (Stem-Cell Technologies, Canada). The iPSC-derived BBB model was built as previously described. Cytotoxicity of A-pBuOzi-A/ATV or DMSO/ATV against hiPSC-derived endothelial cells (hiPS-ECs) was evaluated for three concentrations (45 µM, 90 µM and 150 µM) after 4 h and 24 h incubation. After treatment, CellTiter-Glo® 3D Luminescent Cell Viability Assay (Promega, Mannheim, Germany) was performed according to manufacturer's instructions. Luminescence was measured with an Infinite M200 fluorescence reader (Tecan Group, Switzerland). Cytotoxicity studies were performed in triplicates and three independent biological replicates and results are presented as means ± SEM.