The 1D C CP ssNMR spectra of human cartilage showed enhancement values (ε) of 22.5 -34.5 under DNP. Even accounting for depolarization effects that can cause overestimation of the true enhancement values, we can conservatively estimate a time-saving of one to two orders of magnitude. The effects of formalin-fixation was investigated (Supplementary Fig. ) and found to not interfere with HGA-derived pigment signals.