The possibilities of finding vesicles in the region where the total concentration was less than 3 mM were most promising. Therefore, in AL 6, half of the samples (24 samples) that had the highest probability and the other half that had the lowest probability of forming vesicles were chosen. The composition of those samples can be seen in Figure . Of the 24 samples predicted to have the highest probability of vesicle formation, only 1 did not have vesicles present (4% false positive). For the 24 samples having the lowest probabilities of vesicle formation, only 2 samples were observed to have vesicles (8% false negative rate). Thus, the experiment results are consistent with the model prediction, which demonstrates the ability of the GPC model to extrapolate to low concentration conditions.