To further determine the absolute stereochemistry of 1, which possesses three consecutive chiral centers (C1, C10, and C14) and two trisubstituted double bonds (Δ 2,3 and Δ 6,7 ) (Fig. ), 1 was subjected to crystallization. Thanks to our efficient MKI4 system, enough 1 can be easily obtained for direct or epoxidation-associated crystallization . Fortuitously, a single crystal of 1 was observed even by directly leaving a large amount (~400 mg) in the fridge (-20 °C), consistent with its nonflexible characteristic. X-ray diffraction analysis of this single crystal of 1 (Methods and Fig. ) allowed us to confirm the planar structure proposed by NMR data.