For low-THC cannabis, joints were prepared using 1 g of cannabis per joint. Using a routine analytical cigarette smoking machine according to ISO 3308:2012 (RM 20 H, Borgwaldt, Hamburg, Germany) the joints were smoked without prior conditioning and the combined smoke of 5 joints adsorbed on a filter paper (92 mm). The filter paper was then extracted with methanol and the extract was analyzed using LC-MS/MS .