HCT116 cells were cultured in DMEM culture media with 10% FBS, and 0.1 U/mL penicillin and 0.1 mg/mL streptomycin under 5% CO2 atmosphere. For cell viability assay, 3000 HCT116 cells were cultured in the each well of 96-well cell culture plate and were grew for 24 hours in complete DMEM medium before incubation. Then, DMEM medium was removed, and 100 L