The aim of this work was to evaluate the applicability of microflow chromatography combined with a multinozzle emitter featuring the throughput capability of a narrow-bore setup with a sensitivity comparable to nanospray ionization. The μF/mnESI measurements were achieved with a method run time of 19 min, shorter compared to those reported for nanoflow lipidomics methods. Retention time variations across the sequence were consistent with those in the existing literature and the observed drift could be effortlessly corrected in the preprocessing step. Similar drifts were reported by He et al. with their nLC/NSI setup . Peak broadening constituted the main issue observed with the current setup, especially for polar lipids. We attribute it to the aqueous ligand of the stationary phase, which could interact with polar heads of the lipids. This observation was further supported by the fact that these effects were significantly less pronounced for neutral species. To note, the Waters M-Class LC used in this work was outfitted to minimize non-interactions, featuring an MP35N alloy loop and inactivated PEEKsil material for the autosampler needle and fluidic connections.