We devised two alternative TLIS to reduce the amount of D2O solvents: (a) CCl4/D2O/C6H14 and (b) CCl4/ D2O/C6H6 respectively. From the set-up CCl4/D2O/C6H6, the SEN 13 C spectra of a sample of sucrose (4.66 mg) in 1.8 and 1.3 cm samples (panel B & C in Figure ) showed a ≈1.8 fold and ≈2 fold S:N enhancements relative to the standard volume sample (panel A, 4 cm D2O). The reduced-volume sample comprising of single layer 1.8 cm D2O was also investigated and displayed in Figure , which showed ≈1.2 to 1.5 fold enhancement in S:N for various peaks but the peaks were slightly broader and auto shimming procedure were longer (5 minutes instead of regular one minute, and it may even fail occasionally).