Sequence alignments of the top 500 sequences from the final selection round then allowed us to identify consensus peptide sequences that were divided into distinct families. To validate the identified sequences, we selected two of the most abundant hits, Bpa-P1, Bpa-P2, which accounted for 32% and 5% of the total sequencing reads recovered in round 5 respectively (Figure and). We synthesised both unlabelled (Bpa-P1, Bpa-P2) and fluorescently tagged (Bpa-P1-FAM, Bpa-P2-FAM)