With the initial method established, we focused on automating the most labour-intensive aspect of the assay: the preparation of the multi-well plates. The final composition of each well was standardised, and all volumes were scaled down to one-third to accommodate the smaller capacity of 384-well plates compared to 96-well plates. We developed a liquid dispensing protocol using the Opentrons OT-2 liquid handling robot, with the goal of minimising pipetting errors and enhancing data throughput by utilising 384-well plates. The protocols were crafted using the Opentrons Protocol Designer, and the method files are provided as supplementary material to this article.