Figure shows the cross-sectional fluorescence images of AAO after incubation with NBDliposomes, where many bright spots were clearly observed on the membrane. Moreover, the spots were distant from each other and located on longitudinal axis of AAO pores. These results indicate that NBD-liposomes were immobilized inside the AAO pores without obvious rupture and fusion. We then investigated the leakage of the immobilized liposomes during the storage of the AAO membranes in the buffer. The fluorescence analysis of the supernatant revealed negligible leakage of the liposomes when the membranes were incubated even for one week at room temperature (Figure ). Hence, the liposomes immobilized in the AAO pores are not spontaneously released from AAO membranes incubated in the buffer.