Figure shows the cavity formed in the center of the protein. The figure illustrates the difference between the two cavities; one with glutamate is smaller, and the cavity becomes larger in the absence of glutamate. This is because, due to the presence of glutamate, the amino acids surrounding the cavity come close to interacting with glutamate and cause the cavity to shrink. This change in the binding site has also been observed in the GpdQ enzyme. The amino acids that form interactions with glutamate are Ala496, Arg667, Asp668, Glu422, Glu719, Ile420, Pro497, Tyr470, Tyr745, and Val666.