The order of salt stripping and tautomeric canonicalization is reversed in OpencanSARchem pipeline compared to the canSARchem, with salt stripping performed before generating the canonical tautomer. This was chosen as most molecules in ChEMBL, BindingDB, and other databases are not transformed during tautomeric canonicalization, and by desalting first in our pipeline, we aggregate more molecular and bioactivity information sooner within the hierarchy. To determine if the order affects the resulting canonical tautomers in OpencanSARchem, we first extracted the molecules with more than one fragment after standardization step and excluded the inorganic compounds. This subset represents 4.8% of the total records in ChEMBL 32. Then we compared the results when performing tautomerization before or after the salt-stripping.