Folding module The folding module wraps the ColabFold package to perform reduced-MSA AlphaFold2 inferences. MSA generation was performed using the ColabFold MMseqs2 web server. The user can specify the MSA depth based on their requirements. Generally speaking, a lower MSA depth increases structural diversity but reduces confidence. We also find that sampling larger conformational changes requires shallower MSA depth. Additionally, structures generated with different MSA depths can be combined.