In situ 1D fiber growth 50 µL of 2.51 nM (0.1 µg/mL) PCL 45 -b-PDMA 348 seed solution was spin-coated onto the cleaned coverslip twice at 3200 rpm for 50 seconds, immediately followed by 4000 rpm for 30 seconds. A cleaned plastic spacer was then placed onto the seed-coated coverslip to form a reaction chamber. This reaction chamber has then been mounted above the objective with the objective being adjusted to focus on the surface (Fig. ). PCL 73 -b-PDMA 204 unimer stock solutions were diluted in methanol to achieve the targeted concentration (0.06 µM, 1.67 µg/mL) and added into the reaction chamber, followed by iSCAT imaging immediately.