Evaluation of pH stability, thermal stability, and reusability of BNNS-Arg@Lac. The catalytic ability to oxidize ABTS was used to evaluate the pH stability, thermal stability, and reusability of BNNS-Arg@Lac. BNNS-Arg@Lac and natural laccase were incubated in ABTS solutions with pH values ranging from 2.5 to 7. The relative activities at various pH values were normalized to activities at their optimum pH (pH 3). To determine the thermal stability, temperatures from 20 °C to 70 °C were utilized and both BNNS-Arg@Lac and natural laccase were incubated at certain temperatures for 10 hours. For the reusability test, 20 mg of BNNS-Arg@Lac was incubated with 2 mM ABTS and reused by high-speed centrifugation (20,000 g).