P-SPR is a re-emerged and important topic, now it is possible to synthesize them on a large scale and generate analog series from an interesting initial sequence. But, the synthetic methods have been updated more rapidly than the peptide design area. Although different efforts to reduce this methodological gap have been developed, it remains challenging to fully decode the P-SPR since it can be highly complex in nature. Interestingly, peptides could resolve a large list of clinical, biological, chemical, pharmacological, alimentary, and agrochemical current issues . For this reason, it is important to use novel approaches to quantify and understand, as much as possible, their SPR.