Table . PhoLip_ATPase domains of monkeypox virus E protein F13 Downloaded phospholipid transport related sequences from the Uniprot database were compared one-by-one with the F13 protein from the monkeypox virus using the built-in MEME program. There are PhoLip_ATPase_N and PhoLip_ATPase_C domains in the F13 protein of the monkeypox virus, as shown in Table . There is some duplication between PhoLip_ATPase_C and PhoLip_ATPase_N. The active site of phospholipid transport (flipse) of the EF13 protein can be located at positions 115-417 by combining the ranges of PhoLip_ATPase_N and PhoLip_ATPase_C. However, this region did not overlap with the PK_Tyr_Ser_Thr A region and contained no Mg2+-ATP or Mg2+-GTP binding site. This result suggests that Mg2+-GTP may still be involved in the conformational change of PhoLip_ATPase. There's a chance that cysteine proteases help create the fusion pore. Two cysteines can be found in PhoLip_ATPase_C C. Fusion loop formation and lipid turnover in viral and cellular membranes are both regulated by the PhoLip_ATPase domain, which is responsible for membrane destabilization.