A 1.0 mL/min flow rate was used, and the column compartment was set to maintain 50.0°C throughout the experiment. Little difference in chromatographic quality was observed at lower flow rates (0.800 mL/min), though the high flow rate facilitates an increased sampling rate. The variable wavelength detector (VWD) used is a high sensitivity detector that sacrifices observation of the entire UV-Vis spectrum of an analyte for sensitivity and is intentionally set to monitor a characteristic wavelength for both target analytes.