3.0 (Waters Corporation, Wilmslow, UK). To minimise inclusion of false positive and/or non-quantifiable peptides, peptide threshold parameters based on previously optimised values were applied . This included minimum intensity of 1000, minimum sequence length of 5, maximum sequence length of 30, minimum products of 2, minimum average products per amino acid of 0.11, minimum consecutive products of 1, minimum sum intensity for products of 472, minimum PLGS score of 6.62, and minimum precursor MH+ error of 5 ppm. Furthermore, peptides were only retained if identified in 3 out of 4 LC-MS replicates with an RT RSD of ≤4%. For HDX-MS, all peptides were manually validated on DynamX using default settings. For combined 1-pass/multi-pass data, DynamX output files were concatenated, and redundancies removed to create "combined" cluster files containing both reference and deuterated peptide data. DynamX cluster data and heat maps were then exported to Deuteros ver. 2.0 and the HDXviewer webserver for data interpretation and visualisation.