Experimentally, the pore size is estimated to be 0.96 ± 0.05 nm from rejection data using the Ferry model. However, this is a single fit to a pore flow model, which is not entirely applicable in a flexible, sub-nanoscale pore. The size of a sub-nanoscale pore depends on how it is defined and measured. For example, the geometric radius of a circular nanoscale pore Insets show snapshots of the simulation (green beads for glycerol molecules and white surface for gyroid minimal surface) at the start (same for all three temperatures) and the end of the equilibration. At 0 ns, glycerol molecules are mostly along the gyroid minimal surface. For T = 300 K, glycerol molecules are still mostly around the gyroid minimal surface even after 1000 ns. However, for T = 343 K and T = 273, the structure is no longer gyroid after 200 ns and 950 ns respectively. may not represent the actual area available for particles to translocate through the pore due to surface interactions, such as steric hindrance closer to the pore edge. Conversely, atoms making up the pore boundary may fluctuate to open up the pore for larger particles and thus increase the effective pore radius.