We explored the effect of different solvents and vitrifying methods to mitigate the MQCO dampening effects seen with MQ-SC of ATP. We first explored utilizing DMSO instead of glycerol as a glassing solvent in a 77:17:6 DMSO d 6 :D 2 O:H 2 O ratio , which prolonged the long T 2 component from 12.95 ms (in glycerol) to 13.56 ms (in DMSO) (Figure ). We observed a minimum of 3 MQCOs across the alpha, beta, and gamma chemical shift environments for ATP in DMSO when we applied cosine frequencies of 1 through 3 (Figure . All experiments were taken with a relaxation delay of 6 s. Monophosphate breakdown can be viewed in Figure . Conventional SC analysis, along with additional solvent comparisons, can be viewed in Figure and S20.