Psilocybin production is now possible on a significant scale. The synthesis of psilocybin first described by Hoffman was recently refined for a larger scale by Shirota et al. . New methods of biosynthetic production from tryptophan, for example, have been described recently . Bioengineered production was also determined to be feasible using E. coli, S. cerevisiae, and systems that combine chemical synthesis with enzymatic catalysis within a cell-free hybrid reactor . Methods of larger-scale production are available to meet newfound interest in psilocybin and rapid techniques for the assessment of purity on modern instrumentation are desirable. New psilocybin products currently being developed must be evaluated for purity to ensure consistency and quality of research.