We note that when comparing local (microscopy) and bulk ( 1 H-NMR) assessments of lipid oxidation, one should consider the spatial resolution limit of the imaging technique used. In our CLSM experiments, the smallest droplets in which BODIPY 665/676 fluorescence could be quantified were ~ 1 µm in diameter. In our polydisperse WPIstabilized emulsion, ~ 14 vol.% of the oil was present in droplets smaller than 1 µm and ~ 1 vol.% in droplets smaller than 0.2 µm, which represents the conventional resolution limit of CLSM. It was shown that more lipid oxidation products were present in the smallest droplets, which implies that lipid oxidation is underestimated if only droplets larger than 1 µm are analyzed by CLSM. The oxidation in tiny droplets could be further studied with super-resolution techniques which allow resolving features smaller than 200 nm. We further note that a decrease in native fluorescence over time can also be caused by the dissolution of the dye into the continuous aqueous phase. Such an effect can occur when an emulsifier such as Tween 20 is present in a concentration high enough to dissolve the dye (Figure ). Additionally, further attention is needed for using BODIPY 665/676 in protein stabilized food emulsions as many proteins such as bovine serum albumin (BSA) in WPI have some minor binding ability of lipids which can partly participate in transporting lipids.