The experimentation for substrate scope screening and reaction kinetic study steps was conducted using the Opentrons OT-2 liquid handling workstation. In the OT-2, modules including the pipette module (P300 GEN2, 20-300 μL) for liquid transferring, heater-shaker module (200-3000 RPM, 37-95 °C) for enhancing mixing of reaction mixture, and storage module for storing reaction stock solutions. Operation codes, generated by the Hardware Executor, were uploaded to the OT-2 via its desktop application or a Jupyter notebook to initiate automated reaction execution.