The antibacterial activity of Zn-PMA was assessed using the agar well diffusion method at three different concentrations (30 mg/mL, 20 mg/mL, and 10 mg/mL). The results showed a dose-dependent inhibition, with larger inhibition zones observed at higher concentrations (Table ). At 30 mg/mL, Zn-PMA exhibited the highest antibacterial activity, with inhibition zones ranging from 4.5±1.0 mm (Escherichia coli) to 6.0±1.0 mm (Listeria monocytogenes).