The size distribution of LUVs suspension was measured using dynamic light scattering (DLS) setup Zetasizer Nano ZS (Malvern Instruments, Worcestershire, UK) consisting of a He-Ne laser (633 nm) and an avalanche photodiode detector (APD). To get the optimal scattering intensity, the sample was diluted to the 0.2 mM final lipid concentration, transferred to a plastic disposable cuvette (Brand, Wertheim, Germany), and measured after 2 minutes of equilibration time at 298 K. Scattered light was collected at the angle of 173° and intensity-weighted size distribution was obtained using Zetasizer Software 7.11.