According to Table , structural proteins (S, E, and N) and non-structural proteins (ORF3a, ORF6, ORF7a, ORF7b, ORF8, ORF10, and nsp3) contain thiol-activated cytolysin domains (Thiol_cytolys_C, IPR035390). This cytolysin domain is absent from the M protein. As shown in Table , the Thiol_cytolys_C motifs of S, N, ORF6, ORF10, and nsp3 lack cysteine (C), indicating that they lack thiol-activated cytolysin activity. "CAYCC" refers to the E protein's non-transmembrane heme-binding domain. E's Thiol_cytolys_C motif contains the heme-binding domain. E is capable of forming pores in the membranes of viruses and infected cells (multi-inclusion bodies). Then E cannot puncture the membranes of uninfected red blood cells directly. ORF7a and ORF7b have cysteine-containing Thiol_cytolys_C motifs, with ORF7a having an unusually short motif. There is no evidence that these two proteins can form pores. Therefore, these two proteins cannot function as cytolysins.