Heat-Cool Measurements. 3 mL of all concentrations of 2NapFF-Na (5-100 mg/mL) and 2NapFF-TBA were formed as described above. 2 mL were then pipetted into 14 mL glass vials and then placed into an oven set to 60 °C for 1 hour. After an hour, solutions were allowed to cool undisturbed on the bench at room temperature over two hours. These were then inverted and monitored for instability every day for a 14 day period or used for analysis.