Rule of Three (RO3) has been useful in ensuring that fragment libraries really do consist of compounds with active fragment-like properties . The parameters of 'Rule of Three' are such that a molecule with molecular weight ≤300, the number of hydrogen bond donors is ≤3, the number of hydrogen bond acceptors is ≤3, ClogP is ≤3, NROT (≤3) and PSA (≤60) would be an active fragment. Any fragment that passes these rules on the average could be useful when constructing fragment libraries for efficient lead discovery . The RO3 was applied to the fragment library to assess whether our derived active fragments possess the parameters to exhibit active fragment-like properties. All the fragments passed the RO3 as indicated in