Particle sizes were determined using the Mastersizer 3000 laser diffraction particle size analyzer (Malvern, Worcestershire, UK). The samples were added to the water tank of the Mastersizer while maintaining constant stirring. To ensure accurate measurements, sonication was employed for 60 s to break up any large particles present in the samples. Each sample was subjected to three measurements, with each measurement repeated five times to obtain reliable and representative results. The Mastersizer 3000 software was configured with specific settings for the analysis. In the software settings, the refractive index of gelatin was selected to account for the optical properties of the sample. Additionally, the software was configured to consider the non-spherical nature of the particles being analyzed. This data is then analyzed to determine the particle size distribution of the sample.