In order to demonstrate how an automated analysis can shed light onto the presence, formation and evolution of defects in supramolecular polymers, as a case study here we focus on systems based on the BTA architecture, for which our fine CG models recently suggested that the presence of local defects is of key importance. In particular, we start by considering two variants of BTA supramolecular polymers that are soluble in organic solvent and in water (Figure ), and we will refer to these two systems respectively as ( ) and ( ). The (1) monomers differ from the (2) monomers only in the side chains, that are lipophilic in (1) and amphiphilic in (2). We consider BTA supramolecular fibers composed of 80 ( ) or (2) monomers modelled using the CG models of Ref. polymers in an aqueous medium reveals a complicated, non-uniform structure: these fibers contain branches in their backbone (or defects in the stacking of cores) as well as free monomers that are adsorbed on the fiber surface (Figure ).