For the F13L1 consensus, top-children were selected predicting both DWBEL docking-scores < -85 and ADV affinities < -11 kcal/mol to yield 32 topchildren consensus. Representative 32 top-children targeted similar F13L1 docking-cavities (Figure The identification of the F13L1 and F13L2 amino acids surrounding their docked complexes with the DWBEL+ADV consensus top-children, confirmed the visualized differences among the representative top-children scaffolds (Table ). Previously unreported, the docking to the F13L1 and F13L2 mutants containing the pooled mutations isolated from Tecovirimat-resistant patients, identified some new possible targeted amino acids, compared to previous studies. Nevertheless, there were many coincidences with some of the amino acids previously reported to be targeted by Tecovirimat docked to the MPXV F13L isolated in 2007 . For instance, the new top-children targeted amino acids 312 N, and 334 H 338 H located at the near canonical phospholipase-like motif and their Histidine putative requirements, some of them predicting Hydrogen bonds (Table , red H). Additionally, one of the top-children (35702), predicted Hydrogen bonds to 283 D, one of the amino acids mutated in resistant MPXV and previously isolated by in vitro resistance to both Tecovirimat and IMCBH .