The virulence nature of the EHPs was predicted using MP3, VICMpred and the VFDB server. The MP3 server uses an integrated SVM-HMM approach to provide improved efficiency and accuracy to analyze proteins of pathogenic sources for both metagenomic and genomic datasets . VICMpred server with an overall accuracy of 70.75%, employs SVM-based methods having patterns, amino acid and dipeptide composition of bacterial protein sequences . VFDB server performs an iterative and exhaustive sequence similarity searches among the hierarchical prebuilt datasets using VFanalyzer pipeline to accurately identify potential pathogenic strains .