We next explored glycosylation capability of our donor panel (compounds 4, 6, 9 and 10) with D-GalN acceptors 17 and 18. Glycosylation of thioglycoside donor 4 using N-Troc acceptor 17 and an NIS/TfOH promotor system afforded disaccharide 19 in 75% yield (Scheme 3). The reaction proceeded smoothly, and isolated yields were consistent on >2.0 g scale. The desired b-(1,3) glycosidic linkage was confirmed through a large JH1'-H2' coupling constant of 8.0 Hz ( 1 H d = 4.83 ppm). A H1'-C3 HMBC correlation further confirmed the desired linkage had formed. The glycosylation conditions were successfully replicated with C4-O-Lev donor 6, generating disaccharide 20 in 60% yield. Switching to N-TCA acceptor 18 and donor 4 furnished disaccharide 21 in 68% yield and glycosylation of 18 with donor 9 delivered disaccharide 22 in 74% yield. Finally, use of C4-O-Lev protected donor 10 afforded disaccharide 23 in a slightly lower 63% yield. From these results the reactivity of an N-Troc D-GalN acceptor was established as comparable to an N-TCA derivative.