The integrity of the GUV membrane, specifically in relation to their MOF anchoring capability, was investigated using POPC:POPG:Chol lipid mixture. This stability of immobilized GUV@MOF was assessed at 2 and 6 hours when stored at room temperature, and over a 12 hour period when stored at 4°C. Copper and zirconium based MOFs, with dicarboxylate ligands demonstrated remarkable GUV immobilization capacities, with anchored vesicles remaining intact when imaged after 2 hours (Figure , 4h), 6 hours (Figure , S18a) and 12 hours (Figure , S18b). Similar results were observed with tricarboxylate ligand MOFs HKUST-1 and MOF-808 after 2 hours (Figure ) and up to 12 hours (Figure ). This extended stability is attributed to the excellent water stability of these MOFs, which prevents structural dissociation and ensures prolonged anchoring. The smaller particle size of the MIL-53 MOFs resulted in impotent GUV immobilization. While some GUVs anchored to MIL-53(Al) particles were stable for up to 2 hours (Figure ), MIL-53(Fe) particles showed variable behaviour. We observed individual MIL-53(Fe) particles and clusters, with GUVs preferentially immobilized by the MOF clusters. The GUVs were anchored when imaged after 2 hours (Figure ) and remained attached for up to 6 hours (Figure ), while those associated with smaller MIL-53(Fe) particles displayed some mobility.