Based on the results presented in Figures and, we would argue 10 replicas (5 pairs of replicas from each state) to be enough to obtain quite reliable and reproducible results for both systems and decided to use this number of replicas for an additional bootstrapping/subsampling investigation. In this investigation, we tested altering the sampling rates for the KIF calculations. For example, in the case of PTP1B, frames were originally analyzed with PyContact 1 and then KIF using a time spacing of 0.2 ns.