The soil was collected from three different districts of Pakistan including Faisalabad, Sheikhupura, and Gujranwala. Soil samples were taken during the crop growth period to assess the nutrient status of the soil in which plants are actively taking up nutrients. Soils were collected at depth of about 20 cm. However, in some cases, especially those areas in which irrigation continuously effect, the sample was collected to a depth of 60-100 cm for monitoring nitrate (NO3-N) leaching. Soil samples were poured into pots (6 cm) for each district. The average temperature of soil was 20.5 ℃ with an annual rainfall of 1470 mm. The mean pH, bulk density, soil total nitrogen, and soil organic matter were measured as 7.10, 1.25 g/cm 3 , 1.2 g/kg, and 28.9 g/kg, respectively. While the mean ammonium and nitrate content was recorded as 3.90 and 1.37 mg N/kg, respectively .