Picosynechococcus sp. PCC 7002 growth was performed in Media A+. Liquid cultures were grown in a custom Kuhner shaking incubator at 37˚C, 250µE/m 2 /s light intensity, and a constant 1% CO2 atmosphere. Plate cultures were grown on media A with 1.5g/L Bacto agar, and incubated at 37˚C, 200µE/m2/s light intensity, and a constant 1% CO2 atmosphere. Genes of interest were cloned from Synechococcus elongatus UTEX into E. coli shuttle vectors along with a gentamycin resistance cassette and 750bp of homology to the 7002 genome up-and down-stream. Constructs were assembled using isothermal assembly, propagated in E. coli, then purified for transformation into 7002. 7002 was grown in media A to OD730 of 0.5-1.0, then centrifuged and resuspended in 1/10 th volume. To this concentrated stock, 0.5-1µg of DNA was added to 1mL of concentrated cells, then incubated for 3 hours at 37˚C with 200µE light intensity without shaking or CO2 addition. After transformation, cells were plated on media A+ agar plates with 10µg/mL gentamicin. After 1-3 days, colonies were pooled into liquid media A+ with 30µg/mL gentamicin, grown overnight, and plated on media A agar plates with 30µg/L gentamycin. Individual colonies were screened via PCR to ensure they were homozygous and patched if needed onto subsequent media A plates with 30µg/mL gentamicin.