As shown in Table , compound 5 with two methoxy groups displayed a good binding affinity to NSD2 PWWP1 domain, with an IC 50 value of 4.44 μM，which is much better than MR837 (4). This encouraged us to synthesize analogs 6 -12. According to the binding assay, removal of one or two methoxy groups led to compounds 6 and 7