To further investigate the organelle targeting ability and location of Nuc-DAOTA + (λex = 560 nm, λem = 600 nm) in live CHO cells, co-staining experiments were done with the commercially available Mito-Tracker green (λex = 488 nm, λem = 512 nm) and nuclear stain Hoechst 33342 (λex = 405 nm, λem = 455 nm). It was clearly demonstrated that the red fluorescence of Nuc-DAOTA + (Figure ) and blue fluorescence of Hoechst (Figure ) selectively located in the nucleus and the strong Pearson correlation coefficient (R) of 89% obtained from the overlay image confirmed their extensive co-staining (Figure ). Further, confirmation was done from the well separated red fluorescence of Nuc-DAOTA + from nucleus (Figure ) and green fluorescence of the Mito-Tracker green from the mitochondria (Figure ) and poor co-localization with R = 23% was shown in the overlay image (Figure ). This was also evident from the intensity profiles obtained along the nuclei of CHO cells as region of interest (ROI) indicated as white dotted-lines in the overlay images (Figure