Overall, this model was developed to mitigate this issue as much as possible, while still maintaining correct interaction balances. Further ongoing development focused on the lipid models, will continue to mitigate this issue by tackling some of these factors, in particular interactions with the PO4/GL layer. These changes should lead to a slight decrease in the center plane cholesterol population through an increase in the flip-flop barrier, when coupled with future iterations of the Martini 3 lipidome.