It is known that the ROS expression during seed germination is strongly associated with genes involved in ROS metabolism . In Vicia faba L grown under alkaline conditions, the total expression level of the ROS metabolism-related genes (GO: 0072593) [35] was 88.5% of the level that in water (Fig. ). Conversely, Sweet crispy showed higher expression level of these genes compared to Vicia faba L., which also displayed approximately 2 times higher of these genes in alkaline environments compared to natural water.