The total synthesis was completed by first coupling fragments A and B, and fragments C and D. The resulting two larger peptide fragments were then joined, eventually yielding the complete peptide backbone. A notable step was the facile saponification of the methyl ester in peptide fragment AB, as it was accompanied by the elimination of the nitro groups and the formation of the two dehydrovalines. Once the complete peptide chain was available, the synthesis was completed by mild removal of the Boc group, amidination with reagent 7, and global removal of all allyl-bearing protecting groups. This synthetic route was also used to prepare the originally reported structure of MyxA, which is described in the SI. NMR and LC-MS analyses of both synthetic products, as well as comparison with a natural sample of MyxA, confirmed that the structure proposed here is the correct one and that the published one must be revised.