An HME deadstop experiment was performed and samples were collected at different positions inside the cooling die, to capture anisotropic structure development. Using high-resolution confocal microscopy (Airyscan & RCM) we took various images of the deadstop sample taken at 21cm into the cooling die. The sample was imaged in an area of 0.6 mm by 6 mm (sagittal orientation) ranging from close to the edge of the cooling die to the centre. Regions with sub-micron protein fibrils (white, high signal intensity) are interleaved with water and cellulose domains (black, low signal intensity). The skin region (Fig. ) contains protein fibrils aligned with the flow direction (Fig. ). The core region does not show any apparent alignment (Fig. ). The mean WOP of the skin and core is 0.26 ± 0.33 and 0.08 ± 0.24 respectively, confirming an overall higher ordering in the skin region similar to what we showed in