The previous promising experience with Semantic Web and the involvement in the NanoSolveIT EU project set the aim for this work to focus on establishing a semantic interoperability framework for nanosafety data that goes beyond a single project or a dataset, covering different bioassay types, namely, cytotoxicity, transcriptomics, and physicochemical, and enriching it with provenance metadata. Morover, applying the framework to readily available datasets to maximize their FAIRness, promote their reuse, and enable their integration for deeper insights and better understanding of nanomaterials safety. More concretely, this work aimed to address the following points: 5. Conducting targeted queries to generate insights from the interlinked datasets in the knowledge graph, followed by demonstrating integration with external sources.