Previous research has documented amino acids in grasshoppers Research on scorpions has also confirmed the presence of amino acids . found lysine and glutamic acid to be the most abundant in Scorpaena porcus, Linnaeus. Other studies have documented amino acids in scorpion venom, while identified aspartic acid, histidine, alanine, tyrosine, and cystine in scorpion hemolymph through hot water extraction compared to ethanol-based methods. Glutamic acid is particularly crucial in arthropods. Among the arthropod hemolymph samples analyzed, the highest glutamic acid concentration was observed in scorpions (class Arachnida), followed by spiders within the same class. These results are consistent with findings by , who reported glutamine as the most abundant amino acid in spiders. Additionally, studies by , , and Kuhn-Nentwig and Nentwig (2012) identified high proline levels in spider hemolymph. reported tyrosine and phenylalanine as predominant in Eurypelma californicum spiders.