6.0 mg of Nitrobenzene were dissolved in 5.0 mL THF (9.75 mM). 102 µL of the stock solution were diluted into 2.0 ml THF to make a 0.5 mM solution, from which 200 µL were further dissolved in 1.8 mL of THF to achieve a final concentration of 5x10 -5 M. This solution was measured to determine the absorption spectrum of neutral nitrobenzene, showing a 261 nm band with a molar absorption coefficient of ɛ = 8.9 • 10 3 M -1 cm -1 . Addition of NaO t Bu base equimolar solution (19.2 µL of 5.20 mM) inside the glovebox turned the solution into a pale brown colour. The UV-Vis absorption of this sample presents an extra band at 354 nm with a molar absorption coefficient ɛ = 1.0 • 10 3 M -1 cm -1 , along with the main band at 261 nm ɛ = 9.2 • 10 3 M -1 cm -1 (Figure ).