We further seek to calibrate the ligand-ligand interactions on mixed-ligand NCs with the dynamic volume analysis. Because the C signals of long and short ligands overlap, the investigation is first carried out on selectively deuterated samples to separate the individual contributions. Here, 1 H- C DIPSHIFT is applied to the protonated ligands on the mixed-ligand NCs with either deuterated C22 or deuterated C14(see Figure & S4). As shown in Figure , for the short ligand (C14), the interaction energy (𝐸 ) is slightly decreased on the mixed-ligand NCs as compared to that on the pure-ligand NCs. For the long ligand (C22), the interaction energy (𝐸 ) decreases more dramatically. This is consistent with the general picture obtained with 2 H NMR as seen in Figure .