The separation of tryptic digested peptides was performed on a BEH C18 UPLC column (Waters, 1.7 µm, 2.1×50 mm) using 0.1% FA in water as mobile phase A (MPA) and 0.1% FA in ACN as mobile phase B (MPB). The ZenoTOF 7600 mass spectrometer was operated in fullscan MS (m/z 100 to 1500) with collision energy (CE) set as 10 V. For MS/MS, CE = 40 V was applied to CID, whereas the kinetic energy (KE) for EAD was set as 11 eV. The LC gradient and other parameters are described in the supporting information in detail (Section S2).