This work describes an iterative approach to the design and assay of an immobilized protein kinase. While a prior report has shown that it is possible to immobilize PKA onto solid support, this study did not demonstrate activity of the immobilized preparation towards either recombinant or endogenous PKA substrates . Furthermore, by developing a scalable heterologous expression method, this iteration of a PKA-Halo fusion is likely to be substantially more cost effective.