Suppl. Fig. ). This analysis allowed for extraction of key features separating the respective groups for all animals within a given age group. A limitation of this approach is lack of possibility to visualize the unique features responsible for separation of the fine plaque subcomponents in the context of a single animal. Therefore, we performed a second round of analysis that was based on an Y3 matrix. This approach allowed for extraction of unique features that underlie the separation of background from plaque cores and periphery within individual animals. Here, for the features that allows classification into the respective region of interest (ROI; i.e. plaque, core, periphery) no major difference was observed between the different age groups and between the different animals., confirming the validity of the first analysis based on averaged ROI data.