At higher concentrations of MO-LCNPs, GUVs can become rapidly destabilised and destroyed. Here, the kinetics of MO incorporation into the GUV membrane will increase. We propose that under these conditions the increasing MO concentration in the membrane may pass the lamellar-cubic phase boundary (~75 mol% MO in the MO/DOPC membrane), where the faster kinetics of the lamellar to cubic phase transition occur before the slower dynamics of membrane topological and morphological transitions can occur.