Furthermore, Lewis acids such as transition metals and lanthanoid salts were included as well. A qualitative overview of the observed hydrolysis performances can be gained from the depicted heatmaps and the respective color code (Figure Consequently, instead of considering the absolute fluorescence of a catalyst system at a particular point of time, we rather analyzed the relative fluorescent decay and determined the corresponding half-life through exponential curve fitting (Table ). This approach was highly successful and reliable (see below) for gaining a rough indication of particularly effective catalysts (see champion table and associated kinetic traces in Figure and). From the compounds tested, N-alkylated imidazoles and pyridines with electron donating substituents in meta-or para-position catalyzed the hydrolysis reaction most efficiently. In particular, DMAP was found to be the catalyst giving rise to the fastest fluorescence decay (t1/2 = 1.5 min, Table ). Besides DMAP, 27 other compounds were identified that outcompete pyridine as hydrolysis catalysts (Table ). To ensure that the observed decrease in fluorescence intensity was indeed indicative of enhanced hydrolysis of acylphosphates, the most promising catalysts of Figure were tested as additives in our NaphPO4 reference system under HPLC monitoring as sown in Figure -64. As shown in Figure and S64, the relatively low concentration of 10 mM DMAP was sufficient to decrease the hydrolysis half-life by a factor of 22.