The cell attachment assay has also been performed to assess the attachment of living cells onto the surface of the copolymers films, using L292 cell line. Formaldehyde-based protocol was applied to fix the attached cells onto the surface of the copolymers . The copolymer films were prepared and cut into 5x5 mm squares, which were then placed into a polystyrene plate. Cells with a concentration of 2x10 4 cells/ml were seeded onto the samples and incubated in a CO2 incubator for 24 hours at 37°C. The samples were then gently washed with PBS and fixed using 4% formaldehyde in PBS buffer (pH 7.4). Due to the high autofluorescence of the polymer samples, it was not feasible to use fluorescence microscopy to visualize adherent cells. Therefore, scanning electron microscopy (SEM) was chosen as the imaging technique to assess cell morphology and attachment on polymer surface. After addition of 4% formaldehyde in PBS buffer, the samples were first left for 2 hours at room temperature, followed by another wash with PBS buffer, and then dehydrated using a graded alcohol series from 50 to 99.9%. The samples were then removed from the alcohol, dried in the air, and the SEM images of the surfaces of the samples were captured using SEM TM4000+, Hitachi High-Technology Corporation, Tokyo, Japan.