Figure shows the simultaneous resistive-pulse sensing and fluorescence imaging of a single fluorescent E. coli lipid liposome multipassed 5 times through the orifice of a 111 ± 8 nm radius nanopipette. The applied potential waveform (Figure ) drives the liposome transport in and out of the nanopipette, with the liposome entering at -1.0 V and leaving at 1.0 V. The red shading in Figure indicates when the liposome is outside of the nanopipette and the blue shading is when the liposome is inside of the nanopipette. The resistive pulses associated with liposome multipassing are shown in the i-t trace of Figure .