IREDs were selected and screened against two 1,4-diketone substrates (21 and 22) and four amine partners (i, iii, v, vi) (see Supporting Information for complete set of IREDs and screening results). As a result, we were pleased to obtain all eight N-alkylated 2,5-dissubstituted pyrrolidines, from the combination of the two diketones and four amines evaluated, in good to high conversions within 24 hours (Table ). Scheme 7. Proposed enzymatic cascade for the synthesis of N-alkylated 2,5-disubstituted pyrrolidines and previous reported TA-IRED cascade for the synthesis of 2,5-disubstituted pyrrolidines