Organs-on-chip (OOC) are microfluidic platforms that integrate human microtissues in a controlled microphysiological environment. OOC systems narrow the gap between in vitro and in vivo testing by recapitulating key aspects of human (patho)physiology as continuous perfusion of nutrients and mechanical stress, providing a promising tool for drug development and personalized medicine . Although each type of OOC is different, many of the OOC platforms developed so far consist of an 'organ 'containing cells of the organ of interest and a perfusion compartment that can include blood vessel endothelial cells and/or immune cells. Generally, a porous polymeric membrane is applied between the two compartments to provide structural support for cell layers and to ensure a separation of transport processes by confining convective fluid flow to the perfused channels while allowing for diffusive transport of nutrients, secreted factors, and metabolites between the compartments. Flexible membranes also enable the mechanical actuation and stretching of cells and have been used for emulating peristalsislike motions in gut-on-chip or breathing in lung-on-chip. Similarly, non-porous membranes deflected by gas pressure have been employed to compress or stretch cells in cartilage , heart , and bone-on-chip 34 models. It is known that cells react to environmental cues, thus all the physical properties of the membrane such as roughness , surface microstructure , mechanical strength , hydrophilicity , and porosity have a significant effect on cellular adhesion and membrane biocompatibility. Despite their importance, these aspects are often neglected in OOC in which membranes are typically obtained from commercial Transwell inserts or filter membranes .