Fetal Bovine Serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin) for 24 hours at 37 °C, in a 24-well plate. At the same time, 10×10 3 L929 cells (passages 10-25) were plated per well in a 96-well plate in complete growth medium and incubated for 24 hours in a cell culture incubator. After 24 hours, the medium was aspirated and replaced with 100 µL of extract, followed by an additional 24 hours of incubation (5 technical replicates). Cell viability was then assessed using light microscopy (Delta Optical IB-100, Mińsk Mazowiecki, Poland) and the resazurin viability assay .