A new glucose biosensor was developed in this study by immobilizing GOx on platinum nanoparticles (Pt NPs) decorated reduced graphene oxide (rGO)/Zn-MOF-74 hybrid nanomaterial. This biosensor utilized the advantages of rGO, porous Zn-MOF, and conductive Pt NPs to improve GOx immobilization and mass transport, as well as enhance electron transfer and current density. The GOx-rGO/Pt NPs@Zn-MOF-74 coated electrode demonstrated a linear range of 0.006 to 6 mM for glucose measurement, with a detection limit of 1.8 μM and sensitivity of 64.51 μA mM -1 cm -2 . The biosensor displayed Michaelis-Menten kinetics and allowed fast and accurate glucose measurement in cherry juice. The use of Zn-MOF-74 as a supporting material for biosensor construction allowed for higher enzyme loading capacity and improved stability and reusability. The combination of rGO, Pt NPs, and Zn-MOF-74 demonstrated promising potential for more efficient materials in biosensor applications.