Biofilm Disruption Assay: In the biofilm disruption assay, P. aeruginosa and S. aureus biofilms were first established overnight and bacterial growth was measured at OD600 to ensure uniformity in growth across the plate. The spent supernatant media and planktonic bacteria were aspirated, and the plates were gently rinsed with fresh media three times. Fresh media (108 μL) was again added to the plates and 2 μL of each of the library fractions in DMSO were added in quadruplicate. The plates were incubated under static conditions for 16 to 20 h at 37 °C. After incubation, OD600 was recorded to measure bacterial growth, spent supernatant media was discarded and the plates were rinsed (x 3) with DI water and left for 30 -60 min to air dry at rt. The adhered biofilms were stained with 0.1% (wt/vol) crystal violet (aq) and placed on an orbital shaker at low speed for 30 min. Planktonic cells were washed, the plates were dried (30 -60 min) at rt and then the crystal violet bound biofilms were solubilized in 30% acetic acid (aq). The solubilized material was transferred to new 96-well plates prior to recording the absorbances (595 nm). Active fractions were described as those that did not negatively impact bacterial growth but eradicated >50% of biofilm as measured by spectrophotometric measurements.