EDA analysis has also been employed to predict the interactions between DS and DN as well as complimentary natural base pairs for both WCF and nWCF (nWCFP1 and nWCFO) conformers. vdW energy between DS-DN is found to be the highest for nWCFP1 followed by nWCFO and lowest for WCF. Interestingly, here the interactions between complementary base pairs are similar irrespective of their orientations (Table ). This suggests that as the length of the DNA increases, flexibility and different conformational orientations do not influence the interactions between the adjacent cBPs. Consequently, unlike MUD, the average population of the conformers for MUDL is almost equal. It further suggests that instead of UBP stability, interaction with adjacent base pairs is a major factor for the conformational distribution and population of the conformations (Figure ).