We have seen that on cooling the LCFAs neither undergoes phase transition themselves nor do they allow other lipids to have phase transition. Even the fully saturated LSFAs, present in NM, also behave very similar to the LCFAs. This is intriguing and needs further investigation. Separation of phases is a prevalent phenomenon, highly being observed in the multicomponent membrane systems. It arises due to the sufficiently large structural differences of the lipids, inducing unfavorable interactions among them. Therefore, one can expect the LCFAs, with highly kinked fatty acid chains, to phase separate from the remaining lipids. We have analyzed the distribution of different kinds of lipid inside the membrane at the lowest temperature (250 K) and how their phase behavior is impacted. We have shown the lateral density profile (LDP) of the bilayer for different membrane systems at 250 K averaged over two time frames (Figure ). We have also picturized the distribution of different sorts of lipids based on saturation level and presence of cyclopropane rings in acyl chains at the same temperature (Figure ). We observe that for the NM membrane, the distinct types of lipids are homogeneously distributed in the membrane systems. Some transient domain formation is observed but complete phase separation of the lipids is still absent. Therefore, the LCFAs are fully mixed with other lipids and thereby inhibits phase transition of the latter. Side-by-side comparison between the 2D-densmap (Figure ) and a simulation snapshot (Figure ) reveals that in NM the LCFA-rich regions are more fluidic compared to the regions having the monounsaturated lipids. The LSFA-rich regions in MMS undergoes gel phase, while some fluidity is observed near the LUFA-rich regions. Interestingly, the monounsaturated lipids in MMU undergoes fluid-to-gel phase transition, while the di-and tri-unsaturated lipids are fluidic and a fluid-gel phase separation is clearly observed. Similar phase separation is not observed in NM.