It is most attractive feature if ruthenium connected to through a non-conjugate bridge biomolecules having oxidative quencher, such as Viologens . because ruthenium and oxidative quencher viologens have their spectroscopic versatility. Both the donor (Ru(bpy)3 2+*) and acceptor groups can be probed by a variety of different spectroscopic methods. Cholic acid is one of the primary steroids, it has three hydroxyls groups oriented at positions of C3, C7 and C12 on the polar concave side of the molecule, which is defined as hydrophilic face. Its hydrophobic steroidal backbone is defined as -face. The facial amphiphiles of cholic acid can lead to self-aggregation in solution or aggregation with other specific supramolecular arrangement. Therefore, cholic acid has been widely used as the building block in supramolecular chemistry to transport ions and polar molecules through various membranes. In addition, cholic acid can be potentially used as an adjuvant of liver-specific drugs and absorption enhancers. Similarly, viologens are a well-studied species exhibiting three reversible redox states, possessing valuable electrochromic and electron-accepting properties. because of its properties, viologens have become of great interest as functional materials in a wide array of applications; a few to name include electrochromic devices, molecular machines, and organic batteries . In the combined molecules, if reduction of the biomolecule is desired, a directly photoinduced electron transfer from ruthenium polypyridyl complexes through the bridging ligand, Mebpy-COOH, can be performed. If oxidation of the biomolecule is desired, a flashquench method can be employed . In the flash-quench method, the excited ruthenium polypyridyl complexes have to be quenched from the non-bridged ligands first, followed by the ET through a bridging ligand .