Incubation of Nanoparticles in the absence of shear flow for protein corona incubation experiments, polystyrene latex nanoparticles were dispersed in either PBS (control), or PBS containing FBS (10 %vol-mimicking concentrations used in cell culture experiments) and incubated for 2 hour and 24 hour periods in a temperature-controlled room (37 °C) using gentle end-over-end rotation to avoid particle sedimentation effects.