In recent years, single-cell analysis has emerged as a transformative approach in analytical chemistry, offering unprecedented insights into cellular heterogeneity and enabling the study of intricate biological processes at the cellular level. Cellular heterogeneity is a common feature in almost all biological systems. Beyond genetic differences, it can also arise from non-genetic mechanisms, where cells with similar genotypes exhibit distinct morphological and phenotypical traits . This heterogeneity may stem from diverse factors such as the cell cycle, stochastic variations in gene expression, and interactions with the surrounding microenvironment . In addition to studies of cell heterogeneity, single-cell analysis is needed for other applications, including research of rare cells (e.g., cancer stem cells) , development biology (e.g., cell changes during embryonic development) , and personalized medicine (e.g., analyzing individual cells from a patient for personalized treatment) .