We therefore suggest that resolving the disorganization will improve folding yields and shape consistency. The primary optimizable parameter in this regard is the 12 bt per triplex turn twist assumption, because the global twisting deformations observed in the polyR five-fold bundle suggests that this number is close enough to allow scaffold compaction, but not entirely correct. Indeed, some structural models of parallel tsDNA estimate that its overall twist is closer to that of B-form dsDNA . In addition to design parameters related to structure and sequence, the folding may also be optimized through secondary assembly conditions including ionic strength and temperature. It would for instance be interesting to test triplex-stabilizing buffer agents that are also common in cell nuclei, such as polyamines .