Similarly, we find for ACP even and odd coherence orders up to 10 and 9 at room temperature, and 8 and 7, respectively when dissolved into DNP juice, vitrified and measured at 100 K for the same excitation time. While excitation efficiency decreases with the increasing order of MQ transitions and is further limited by pulse imperfections (Figure ), CSA relaxation (Figure ), and total spin concentration, the loss of coherence orders at 100 K is attributed to a shorter T 2 relaxation time of the vitrified sample compared to room temperature. Shorter T 2 will result in shorter recoupling times and the inability to excite higher order MQCOs of species with partially averaged out dipolar couplings, thus limiting the total intensity achievable for each individual MQCO. This higher MQCO truncation is further exacerbated by individual n-quantum transition T 2 relaxation, which has been shown with EPR MQ-SC to decrease with higher orders .