Interest in the estimation of turnover number (TON) and its frequency is known . A not-too-clear application of the quotient of the catalyst turnover number and the rate constant of spent catalyst replacement and the Lumry-Eyring model for the estimation of TTN are known. In this study, an alternative approach has been derived (Eq. ( )). The equation shows that, for a given concentration of the enzyme, the turnover number, №, is comparatively quantified either by the relation № ≤ 1 or № ≥1 (Table ) for polysaccharide. It follows that № is not necessarily a large figure where polysaccharide with a characteristically large degree of polymerization is the case for any mass Preprint submitted to ChemRxiv, Jun 05, 2023 concentration of the enzyme, but its magnitude is inversely proportional to [E0]. However, № per second gives values that are ≪ 1/s. It is crystal clear that TOF is not the same as the catalytic rate constant, which is often reported as the ratio of the maximum molar concentration of one of the products to the molar concentration of the enzyme.