Although high entrapment efficiencies (>70%) were obtained for both charged and hydrophobic ion paired liposomes, the size distribution and polydispersity between the two formulations differed. Upon rehydration of the charged liposomes their initial size distribution was not recovered, but a disperse formulation (PDI = 0.439 ± 0.056) with larger (potentially aggregated) liposomes was obtained (Figure ). Conversely, hydrophobic ion pairing permits direct incorporation of the peptide within the lipidic thin-film and extrusion of the final formulation, resulting in small and monodispersed liposomes (PDI = 0.236 ± 0.006, Figure ).