Comparator experiments were performed using several RNA concentrations. Here, we define ∆𝑐̃ = 𝑐̃+ , -𝑐̃+ . and 𝑐̃& = 𝑐̃+ , + 𝑐̃+ . . We investigated five types of conditions of the initial RNA concentrations shown in Figure the C-droplet divided first, whereas under conditions (iv) and (v), the B-droplet divided first (Supplemental Figure ). Figure shows the time courses of the division ratios of B-(rdiv_B) and C-(rdiv_C) droplets quantified using the image processing method shown in Supplementary Note S3. These results showed that with higher ∆𝑐, C-droplet division was faster. Note that an increase in 𝑐& caused a delay in the overall reaction, probably because more RNA molecules induced competition in the degradation of RNA by RNase H in the condition of the same RNase H concentration.