For cryoEM imaging, a FEI GII Titan Krios microscope with a Bioquantum K2 (Gatan) was used. Tilt series were collected using Tomo software with defocus set to 3 µm. The grids were exposed to an electron dose of 18 e -.Å -2 .s -1 and 61 images were taken at 2° increments and ×25,000