In order to obtain biochemical evidence for the involvement of mic genes in the biosynthesis of microginins, we sought to heterologously express the mic BGC from M. aeruginosa LEGE 91341 in E. coli. A few NRPS/PKS pathways from cyanobacteria have been successfully expressed heterologously (using cyanobacterial or E. coli hosts). Using a Sequence-and Ligation-Independent Cloning (SLIC) DiPaC strategy, we assembled the roughly 25 kb mic gene cluster from M. aeruginosa LEGE 91341 into a modified pET-28 vector backbone which includes a PtetO promoter and generates a C-terminal gfp fusion for the last gene in the BGC (pET28-ptetO::gfpv2, Fig. ). The cloning strategy was achieved through SLIC ligation of two PCR-derived amplicons (Fig. ). The resulting construct was cloned into E. coli BAP1 strain which has a chromosomal copy of the sfp gene, encoding a promiscuous Ppant transferase. This enables the post-translational phosphopantheteinylation of the several thiolation domains encoded in the mic BGC.