Immobilization of protein on a microcolumn and affinity tests were performed as described previously by Spodzieja et al. . Peptides were added onto a prepared albumin-Sepharose column equilibrated in ammonium hydrogen carbonate, pH 7.4, and incubated for 2h at 25°C. After 2 h, the column was washed with ammonium hydrogen carbonate, collecting the first and the last milliliter of the solution (supernatant and last wash fraction). The peptide complexes with albumin were dissociated by 15 min. incubation in 0.1% TFA, with gentle shaking. The procedure was repeated twice, and the elution fractions were collected. The collected fractions were lyophilized and analyzed using mass spectrometry (MALDI, Brucker).