Rhodamine B. The FACSJazz lasers were calibrated before the samples were run with the BD Rainbow Calibration Particles (8 peaks). Non-labelled TG-beads were dispersed in 5 mL sheath fluid and put through the machine to check the calibration. Then samples TGRhodB100, TGRhodB73, TGRhodB36, TGRhodB17 and TGRhodB1.7 were run through the FACS dispersed in 5 mL sheath fluid, being observed on laser 488 nm with filter 585/29 nm.