Step 1 involves constructing a periodic system of the inverse hexagonal phase of a given lipid composition using TS2CG in combination with MDAnalysis and fatslim. A single cylinder is constructed with TS2CG, with the principal axis aligned with the z-axis. The cylinder is then filled with possible cargo, water and ions using Packmol. The cylinder is then replicated 3 times, such that there is 4 cylinders in a hexagonal periodic box. This system is then relaxed for 10 ns.