Blastp result against non-redundant and SwissProt database showed homology with lipopolysaccharide assembly proteins (Table ). Multiple sequence alignment between the hypothetical protein and other homologous proteins generated NEXUS file in MEGA. To strengthen homology assessment between proteins, down to complex and subunit level, phylogenetic analysis was performed. The phylogenetic tree showed distances between branches and reveals close similarity of the hypothetical protein with WP_046596310.1 Y. pestis homolog while distantly related with NLU15143.1 Serratia liquefaciens (Fig. ). Fig. . Phylogenetic tree showing relationship of AVO28_00330 hypothetical protein with other similar proteins with true genetic distance.