Optimal alignment of pig and human GABA-T was obtained by a shift of +28 from the pig GABA-T amino acid sequence numbering. The classical RMSD was 0.25Å and the maximum entropy RMSD was 0.045Å, indicating that the two structures were very similar. The receptor was prepared for docking by using the MGLTools utilities . A grid of size 20Å x 20Å x 20Å was centered on the active site residues.