Given this result, we asked under what conditions any pH-dependent effects could be recovered. We observed that in the presence of 1.5 M NaCl, KI8 was coacervated at 4 °C (Figure ). This result is expected, as high concentrations of salt dramatically depress the transition temperature of ELPs. With increasing temperature, the hydrodynamic diameter of the phaseseparated particles at pH = 11.2 continued to grow, while the size of the particles at pH = 7.4 remained constant. This is consistent with the hypothesis that at pH = 7.4, KI8 coacervate particles form in a manner that creates a positively charged surface area, resulting in mutual particle-polymer repulsion and thus an opposing force to assembly beyond the observed size plateau. At pH 11.2, a smaller proportion of Lys residues are positively charged, resulting in a lower repulsive force and a greater tendency to associate until an equilibrium is reached at a greater surface area.