After 15 min, the rabbits were anesthetized with continuous inhaling the mixture of isoflurane (RWD Life Science Co., Ltd.) and oxygen. During anesthesia, normal saline (NS) and glucose solution (5%) were used to maintain blood pressure and replenish energy of rabbit models. The cardiorespiratory monitor (RM500, RWD Life Science Co., Ltd.) was used to measure the heart rates, respiratory rates, and oxyhemoglobin saturation of rabbits for real-time adjustment of isoflurane inhalation volume. Using this method, rabbits could safely be held in the anesthetic state for 6-8 hours without mechanical ventilation. Before nephrectomy the model rabbits were injected intravenously with 500 units heparin for systemic heparinization to avoid thrombosis.