Primary sludge samples were collected daily from March 19 to June 30, 2020 between 8 and 10 am at the East Shore Water Pollution Abatement Facility, New Haven, CT USA, as described in . This treatment plant serves an estimated population of 200,000 in New Haven, Hamden, East Haven, and Woodbridge, CT, USA, and part of the service area contains combined sewers. Samples included both liquid and solid fractions (2 to 5% solids wt/wt) of sludge and were stored at -80°C until analysis. We analyzed daily samples from March 19 to April 15, and weekly composite samples from March 19 to June 30. Weekly sample extracts were further combined into 5-week composite samples, which were used for compound identification analysis only. Figure shows the sampling timeline relative to key dates for the pandemic and related shut down.