To produce a solution of 2NapFF requires a predetermined mass of 2NapFF, 1 molar equivalent of a hydroxide (either sodium or tetrabutylammonium hydroxide) with respect to 2NapFF and deionised water to make up the final volume (Table ). Based on calculations to have a 1:1 molar ratio of 2NapFF to hydroxide, solutions of concentration 50 mg/mL or greater of 2NapFF required 1 M hydroxide. All others were prepared using 0.1 M hydroxide. All solutions were formed at room temperature (normally between 22 and 25 °C).