Most of the eutrophication potential impacts are from combustion phase majorly because of NOX emissions. If 1-or 2-year life cycle span of the biodiesel plant is considered than environmental impacts will be much higher than the reference fuel diesel, but if the life cycle span of more than 8 years is considered than significant reduction in global warming impacts can be achieved. However still eutrophication, acidification potential, and human toxicity potential impacts are expected to remain on higher side than the reference fuel diesel. It is observed that even low yield of Jatropha i.e. 450 kg/ha can reduce the global warming potential (~27.7%) but it will increase the impact in other impact categories. Findings of the study shows that biodiesel has the potential of reducing the GWP impact as reported by earlier studies, however it will increase the impact in other impact categories. Study provides the other side of biodiesel life cycle impacts to the decision makers. Study gives a different direction that while making the decision about the Jatropha plantation the regional environment should be assessed carefully to ensure that Acidification, and Eutrophication will not be a problem in future.