Biomarkers that serve as indicators for disease detection are often present at a low concentration (fM-pM) and therefore, require preconcentration prior to analysis. Electrokinetic methods of analyte preconcentration are advantageous for integration into lab-on-chip (LOC) devices because they provide efficient transport of charged species in small sample volumes. Over the past two decades, electrokinetic methods of focusing that employ ion concentration polarization (ICP) and faradaic ICP (fICP) have been developed for enrichment and separation of a wide range of disease biomarkers, including nucleic acids, 2-4 proteins, enzymes, exosomes, and biological cell and for separations in complex biofluids. There have been reports of concentration enrichment ranging from 10 2 -fold for simple devices to even 10 9 -fold for multistage hierarchical preconcentrators that sweep a large fluid volume. Despite the success of these preconcentration methods, some aspects remain challenging. Many existing ICP-based preconcentrators operate at > 100 V, which hinders integration into portable devices. Further, improvement of volumetric throughput, integration with downstream analysis, and the development of strategies to decrease fluidic instability are active areas of research. Fluidic instability (evidenced by size and magnitudes of vortex flow near the electrode), which is caused by steep gradients in electrolyte concentration and electric field strength and exacerbated by increased device dimensions, leads to unwanted mixing, ultimately limiting sensitivity.