The OA nanozyme was synthesized using a customized methodology involving a 'single chelation/polymer entanglement' procedure as illustrated in Figure . Compared to the previous 'double chelation' based nanozyme fabrication described in earlier organic compound-based nanozyme studies (9,10), the particle stabilizing polymer was replaced with the non-functional group contained polymer (polyethylene glycol, PEG), which does not possess chelation activity with the metallic ion. This replacement fosters better inner entanglement compared to the previous polymer used (polyvinyl alcohol, PVA), resulting in smaller physical dimensions due to reduced surface tension. This reduction enhances the interaction between metal ions and chelator-like organic materials, providing possible steric stabilization . Ultimately, this one-pot particle generation process takes up to 3 hours to obtain the final nanozyme. Before the morphological analysis, the transparency of OA nanozyme in the solution phase was verified through the UV-VIS-NIR spectra scanning, along with its constituent materials (Figure ). Morphological analyses were conducted using electron microscopy, including TEM and SEM with EDS (Figure ) to validate the nanozyme generation through the 'single chelation/polymer entanglement' procedure. SEM images showed the OA nanozyme's spherical structure and uniform size, demonstrating the successful generation of homogenous nanoparticles (Figure , Figure ). Based on SEM images, the high yield of OA nanozyme was confirmed, with a physical diameter around 100 nm or below (Figure ), which fulfilled the required criteria. TEM analysis further corroborated the spherical structure of OA nanozyme as shown in SEM and crossconfirmed an approximate scale of nanozyme within 100 nm (Figure , Figure ). For the indirect confirmation of the presence? of all designated organic compounds/amino acids within the corresponding framework, an EDS mapping image was conducted to target elements such as carbon, nitrogen, oxygen, and iron. The mapping images verified the localization of all elements, with nitrogen sourced from the amine group of alanine and iron dispersed within the matrix.