Freeze dried biomass (2.0 g) was used for lipid extraction using homogenization in a chloroform: methanol mixture (2:1, v/v). The homogenization was repeated three times and the extracts were combined and filtered through a 0.22 µm filter. The solvent was then removed using a rotary evaporator (bath temperature = 50 °C) and lipid weight was determined gravimetrically. Conversion of triglycerides to fatty acid methyl esters (FAMEs) and GC analysis Lipids were transesterified and fatty acid methyl esters (FAMEs) were analysed using a previously described method. FAMEs analysis was performed on a Shimadzu Gas chromatography (GC, 2090N) equipped with flame ionisation detector (FID) and connected to a BID 2030 unit using FAMEWAX column (30 m x 0.32 mm ID (inner diameter)). The inlet was held at 25 ºC with a constant column flow rate at 5 mL/min with split injection (1/150). The oven program was held at 150 °C (5 min. hold), ramped to 250 °C at a rate of 10 °C per min. and held at 250 °C for 1 min. Fatty acid esters were quantified by comparison of peak areas of authentic FAMEs standards (Sigma Aldrich CRM47885).