To test the reproducibility of the method in terms of lipid depletion and preservation of LMWM features, 288 replicate aliquots of the pooled human EDTA plasma sample, from three 96-well plates, were prepared using the dSPE protocol and analyzed with the C18 RPC+/-assay. This LC-MS data was processed and %RSD calculated for the detected features. The median %RSD values for C18 RPC+/were 4.5% and 4.2%, respectively (Figure ), showing no significant variation across all detected features passing the dilution series filter. Additionally, PCA was used to examine whether the main sources of variability could be associated with sample preparation parameters. PCA scores plots (Figure ) demonstrated no trends in variation between the samples when colored by addition of suspension (added column-wise using an 8-channel electronic pipette) or addition of the resuspension water (added row-wise using a 12-channel electronic pipette).