The FT-IR spectrum (Figure ) contains the main expected signals for a long chain 𝜔𝜔-hydroxy fatty acid (Table shows the band assignments). The two strong bands at 2923 cm -1 and 2851 cm -1 are associated with C-H symmetric and asymmetric stretching vibrations respectively, deriving from the hydrocarbon backbone. The wide band from 3100 -3600 cm -1 is attributed to the O-H vibration of hydroxy and carboxylic acid functionalities. The band at 1706 cm -1 is assigned to the vibration of an acid carbonyl bond (C = O) with a shoulder at 1697 cm -1 . They refer, respectively, to COOH involved in weak and strong hydrogen bonds. This has been observed in the cuticle layer of growing fruits, which resembles the system in this study being a mixture of unreacted hydroxy fatty acids. As confirmed by the 1 H NMR spectrum, the presence of the epoxide group is noticeable with a signal at 846 cm -1 , confirming the EFA extraction.