As observed in panel 6b on the left, the main interactions are the SC2 beads in SMACs maleic acid with NC3 beads in POPC lipids (blue solid line), with more than two times the amount of SMAC:lipid contacts compared to the rest. This dominant SC2:NC3 interaction accounting for the highest amount of steady state contacts is not unexpected as maleic acid SC2 beads (as parametrised by Orekhov et al. ) are negatively charged (-1) while NC3 beads in POPC molecules are positively charged (+1), as defined for MARTINI, being NC3 beads in POPC the only lipid bead positively charged for this membrane (POPC:POPS:POP2).