In a follow-up study, investigated acid-induced phytoconstituents of Calotropis procera extract combined with ampicillin to combat clinical resistant isolates of Staphylococcus aureus and Salmonella spp. The aqueous extract of fresh leaves and flowers of Calotropis procera reacted with a 1 mg/mL ampicillin solution, resulting in synergy and enhanced susceptibility against resistant strains of Staphylococcus aureus and Salmonella spp., increasing their zones of inhibition from 0 mm to 16.8 mm and from 5.3 mm to 21.4 mm, respectively.