We assessed the ice nucleation activity of the polymers using the droplet freezing technique in the immersion freezing mode. First, we placed 20 mg of each polymer sample into a clean glass vial, followed by the addition of 10 The cold stage gradually cooled from 20°C to -30°C at a rate of 3°C/min. A camera located on the top of the chamber allowed for the real-time monitoring of the freezing process. A schematic image of the droplet freezing technique used in this study is shown in the Supplement Information, Figure . Employing a MATLAB script , we determined the freezing temperature of each droplet from the recorded video, which we then used to calculate the fraction of frozen droplets (𝑓 𝑖𝑐𝑒 (𝑇), Equation ):