It is well known that a real biological samples of EVs feature high degree of heterogeneity. As a consequence, both m/z and the mass correspondence may not be unique due to broad charge and density distributions of the bio-particles in question. Nevertheless, quadrupole pre-separation proposed here will select relatively narrow mass fraction and, if needed, a deflection stage synchronized with the charge detector can be added to finally single out particles with a desired mass or charge as it is done in dust particle accelerators. In the current contribution, we have shown that vesicles may be separated and detected in vacuum employing a mass analyzer setup, based upon two quadrupoles. In comparison to established techniques the obvious advantage of the current approach may be its selectivity as the mass and charge of each individual particle are measured. However, we consider the short separation and measurement time being the main benefit, for it enables high-throughput sampling of vesicles which may be subjected to further analyses. Particularly, in an electrodynamic trap placed downstream the charge detector selected vesicles could be accumulated and irradiated repeatedly by a mid-IR laser pulse. This will result in desorption of proteins from the vesicle membrane and these proteins could in turn be analyzed in an additional mass spectrometer. Thus, our future development of the current concept is going to be directed toward the separation of individual EVs from real samples with the aim to obtain not only EV's signatures (e.g., mass and charge distributions) but also the mass spectra of the cargo proteins carried by them.