Before performing flicker noise analysis, we used the K-mean++ clustering algorithm to classify single-molecule traces corresponding to high and low conductance features into two distinct molecular sub-populations . We then analyzed each molecular sub-population using flicker analysis; the high-conductance feature resulted in a scaling exponent n  1.37, consistent with a through-bond conductance pathway. In contrast, the low-conductance feature yielded a scaling exponent n  2.05, consistent with a through-space conductance pathway (Fig. ). Based on these results, we hypothesized that the low conductance feature arises from molecular junctions formed by electrostatic interactions between the gold tip and the positively charged backbone at one terminus and by the -SMe anchor and electrode surface at the other terminus (Fig. ). To validate this hypothesis, we synthesized a ladder molecule L1'-PF6 with only one terminal -SMe anchor group as a control and determined its conductance using STM-BJ. Indeed, the entire 2D conductance histogram of L1'-PF6 was dominated by a low-conductance feature closely matching the low-conductance behavior of L2-PF6 (Fig. ), which is consistent with the hypothesis of electrostatic anchor formation governing the low-conductance peak of L2-PF6. We further studied the conductance of L2-PF6 as a function of applied bias between 200 mV to 400 mV (Fig. ).