The Hsp40 affinity profile for each chloroacetanilide exposure provides a distinct fingerprint (Tables ). Acetochlor treatment increases DNAJB8 affinity for most (82%) of DNAJB8 clients identified across the mass spectrometry runs (See Figure and Figure ), with 2% of clients demonstrating a greater than 2 fold increase in affinity. We further compared to a previously reported liver ABPP of mice exposed to acetochlor . Out of 338 common quantified proteins, there is no correlation (Figure , R 2 < 0.01) between the extent to which proteins are destabilized according to the Hsp40 assay and the extent to which they lose iodoacetamide reactivity following acetochlor exposure, indicating that our assay is distinct from measuring conjugation. Of the 28 mouse liver proteins that lost iodoacetamide reactivity or alkynylated acetochlor reactivity following mouse acetochlor treatment, 20 were identified in our Hsp40 affinity runs, with 6 demonstrating significantly (q-value < 0.05) increased Hsp40 affinity (SCP2, ACAT1, PDIA3, NNT, HSPD1, and DLD). Given the differences in model system (intraperitoneal injection of mice followed by ex vivo liver excision and alkynylated iodoacetamide labeling vs. human tissue culture) and in the assays, it is encouraging that several of the same targets are found in both studies.