Although the parameters to be set before conducting the assays are common to most instruments, it is advisable to consult the manufacturer's manual in advance since depending on the spectrofluorometer, they may vary. With some equipment, it is possible to obtain the I460/I403 ratio value after each measurement, but it might be necessary to calculate it manually in other cases. This value comes from the emission intensity detected by the instrument at 510 nm when the spectrometer rapidly sweeps (within 300 ms) between the two excitation wavelengths, 460 and 403 nm (Figure ).