The largest determinant of success for this workflow appears to be the RAMD sampling quality which impacts the quality of path CVs and iMetaD sampling. We have found that the overall accuracy can be improved by considering the RAMD simulation time as well as the standard error of the RAMD times. The RAMD literature provides guidelines for tuning the force constant and number of replicas using these statistics. Nevertheless, it is likely that using RAMD for CV discovery is more sensitive to these parameters than the mean simulation time used for ranking residence times with RAMD. We therefore leave precise recommendations on how to tune our workflow parameters for future studies.