We found that the use of TANa buffer caused a decrease in the nanotube melting temperature relative to when they were grown in TAEMg. This suggests a lower thermodynamic stability of the nanotubes formed in TANa buffer: this is an advantage in terms of dynamic reconfigurability of the structures, but could pose a challenge for growing nanotubes at physiological temperatures in biological applications . However, stability could be easily improved by increasing the length of the tile sticky ends. Another approach could be to optimize the buffer composition to include limited amounts of MgCl 2 to enhance thermal stability. Inclusion of MgCl 2 is often also required for proper enzyme activity, for example in the case of in vitro RNA transcription that has been used to generate adaptive responses in nanotube systems . Buffers with a mixture of monovalent and divalent cations may enable isothermal assembly as well as stability in the presence of enzymatic reactions. The conditions used here to assemble DNA nanotubes also enable the isothermal assembly of more complex DNA structures including various types of DNA origami . For this reason, our work indicates that it may become possible to create DNA systems that take advantage of both origami methods , known for achieving arbitrary nanometer scale patterning, as well as of tiling methods , which easily produce micrometer scale assemblies. Strand-and tile-based assemblies also constitute convenient ingredients for algorithmic self-assembly, allowing to program higher-order morphologies through computation. The combination of all these traits may lead to the development of multiscale DNA materials rivaling the complexity and adaptability of biological assemblies. These materials may in turn be made responsive to strand displacement networks or to physical inputs to achieve an even greater level of adaptation.