While in our room temperature experiments the tetramer is found alongside the single cluster, the elevated-temperature data suggests that once the temperature is raised, higher nuclearity clusters are favoured (as expected ) over the tetramer. As a complementary analysis, we also performed refinements in the same manner as for the room temperature reactions, using the five-cluster model for refinements of each time step in the datasets. These initial refinements [Figure ] showed no significant contribution from the nonamer and 11-mer throughout the reactions, meaning that only the tetramer, single cluster and double cluster are present in measurable concentrations. A second analysis was then performed with only these three clusters (i.e. omitting the nonamer and 11-mer) [Figure ]. In these refinements of the heated in situ reactions, we always observe the single cluster first, with the double cluster emerging at later stages; this agrees with our analysis from the peak-tracking data.