In the low-resolution QQQ experiments, the LOQ in the urine of all 105 analytes was calculated with a median of 0.2 ng/mL. The median LOQ was slightly lower in solvent at 0.1 ng/mL (Table , Figure ). T2-Toxin had the worst performance with high LOQ values of 1,200 ng/mL and 890 ng/mL in solvent and urine, respectively. Excluding mycotoxins, the sunscreen ingredient octyl methoxycinnamate (OMC) (59 ng/mL) had the highest LOQ in solvent and in the matrix, the barely retained acrylamide was challenging to detect at low levels (LOQ = 150 ng/mL). The lowest LOQs were obtained for benzyl paraben at 0.00031 ng/mL (solvent) and scopolamine at 0.00045 ng/mL (urine). The median LOQ was approximately eleven times lower in pure solvent and the difference in the median LOQ between LC-MS configurations was a factor of 6 in urine whereby high-resolution measurements had higher LOQs than the QqQ configuration. When analyzing polar analytes such as acrylamide, the column material and chosen analytical instrumentation (LC-MS) were not ideal in this study. The broad structural spectrum of the analytes included in the multianalyte method, however, clearly required a compromise. Independent of the instrumentation used and the sample analyzed, the substance classes with the highest median LOQ values in solvent were disinfection by-products, foodprocessing by-products, and certain mycotoxin categories (Figure ). In particular, the first two classes were not detected at low concentrations and a high background was apparent. All of the disinfection by-products included and one food processing by-product (acrylamide) were not retained on the reverse-phase column and eluted close to the dead volume. This lack of retention resulted in the interference of highlyabundant ions that subsequently explained the poor detection of the analytes and thus the low median LOQ for these classes. This was, however, not surprising given the fact that LC-MS is typically not the ideal instrument platform for these classes. Personal care product ingredients and phytoestrogens were amongst the three categories with the lowest LOQ values for all sample/instrument combinations (<0.2 ng/mL and <0.05 ng/mL for HRMS and QQQ, respectively). In general, the median LOQs for the different categories were one order of magnitude higher in urine than in solvent; albeit, the overall order remained similar (Figure ). For each category, the median LOQ was largely increased in the high-resolution measurements. Only disinfectant by-products and food processing by-products, that were too polar to be adequately retained on the selected C18column, benefited from the higher mass resolution and had a lower median LOQ when analyzed by HRMS. Notably, the results per category were highly-dependent on the chosen representatives and must not be generalized.