Histamine was selected as the target allergic molecule. Histamine powder was dissolved in deionized water and serially diluted manually to create various concentrations of histamine solution. Histamine detection was conducted through the modification of a previously demonstrated colorimetric assay (0.1M sodium acetate buffer at pH 4 + 1mM H2O2), as it partially replaced the portion that was originally occupied by NaAc buffer volume. Thus, histamine solution (10% of the total volume) was added to the microplate column and thoroughly harmonized with a micropipette. ABTS (6mM final) was incorporated as a target coloring substrate like the previous colorimetric assay . The 96-well plate-based UV-VIS-NIR spectroscopy absorbance measurements method (through Biotek 5 microplate reader) is utilized for signal measurement.