Betulin was Soxhlet extracted with heptane as a solvent. 12.35 g of DOB yielded 4.8 g (40 wt. %) of betulin after approximately 40 hours of extraction. The resulting betulin-free bark (BFB) was air-dried before further processing. An accelerated solvent extractor device (Dionex ASE 200, USA) was used to extract the polysaccharide fraction from the bark, following the protocol developed by Le Normand et al. Briefly, 7.53 g of BFB was subjected to three extraction cycles, each using water at 140 °C for 20 minutes. The extracts were freeze-dried but not used further in this study.