This study shows how aptamer-assisted tumor localization can potentially improve the precision of aptamer-assisted bacteriotherapy. By crafting tumor-specific aptamers and by simulations of molecules, aptamer binding to the bacterial protein's process has been simulated. These findings show one of the significant drawbacks to bacterial-based therapies-nonspecific distribution-can be overcome by aptamer targeting, thus providing a pathway for better and more precise treatment approaches. Improving aptamer-bacteria interactions and judging the therapeutic results in various tumor models to test this potentially helpful approach further should be done in the future.