Lateral heterogeneities in lipid organization such as the aforementioned formation of lateral domains or "lipid rafts" have for long been proposed to play a role in bacterial functioning and especially extracellular communication . For bacteria, one here especially has to consider the inner and outer membranes and also the very strong asymmetric distributions of lipids and proteins across the membrane leaflets as well as along different regions of the usually polarized bacteria such as between polar and septal regions. This all is inter-connected to highly curved membrane regions, all in all influencing also action of AMPs . Whether changes in membrane curvature or lipid ordering of fluidity, all these properties can in principle be studied with microscopic techniques such as fluorescence microscopy (using for example environment-sensitive dyes reporting on local lipid membrane order or techniques such as fluorescence correlation spectroscopy measuring local molecular mobility and thus fluidity) or AFM (observing local membrane topology or stifness) . Here, bacterial membranes are difficult to investigate with these tools due to their more complex membrane structure compared to mammalian cells as well as increased difficulty to introduce membrane labels. However, recent data has highlighted progress in this respect . Another issue is that experiments on live bacteria usually entail laboratory environments with high biosafety levels, which are less equipped with high-end observation tools such as advanced microscopes. Here, bacterial model membrane systems such as supported lipid membranes or giant unilamellar vesicles mimicking bacterial membranes through certain instituted lipid and protein mixtures come into play, which also allow to investigate membrane lateral organization and curvature in a more controlled way (e.g. by controlling lipid compositions). While these systems are good models for understanding basic physicochemical properties and functions, they are quite far away from the real complexity of bacterial membranes. For this, research has started to employ membranes or vesicles isolated from bacteria and e.g. examine the influence of external stressors on their organization . Also, Outer Membrane Vesicles (OMVs) extracted from the outer membrane of bacteria have shown reactions to antibacterial agents, depicting the living cells in a good manner . For eukaryotic membranes, insights into the structural complexity and the formation of raft domains are essential for understanding mechanisms underlying AMP selectivity. The lateral heterogeneity of mammalian cell membranes is indispensable for the hemolytic activity of certain peptides , emphasizing that simple homogeneous membrane models cannot adequately capture the nuanced mechanisms underlying AMP toxicity. Therefore, incorporating more realistic and complex membrane models in experiment and simulation, reflective of both bacterial and eukaryotic systems, is critical to advancing our understanding of AMP activity and selectivity.