The PASADENA signals from malate-D2 (see Fig. ) are antiphase which precludes most 1 H imaging techniques, because the application of imaging gradients to spatially encode the spins would cause signal cancellation. A pulse sequence is required to convert the two-spin order into observable, in-phase magnetization, and additionally suppress signals from background magnetization arising, for instance, from water or fat molecules present in significantly higher concentration. For this purpose, we utilize two versions of the out-of-phase echo (OPE) pulse sequence . In one version, OPE-45, a hard 45° pulse nonselectively excites the signals, and background magnetization is removed at the end with pulsed field gradients. In the other version, OPE-s90, a selective 90° pulse is applied to the malate-D2 proton resonance at 2.5 ppm, which does not excite background magnetization. The theory of how these two pulse sequences work is given in the Supporting Information. Figure illustrates the core principle of the experiment and how the pulse sequences operate.