On the other hand, for (EA)4, we observed some deprotonation of D even in the D-B control (Fig. ), indicating that the bridge itself, composed of titratable amino acids, can serve as the PMF initiating the PT process. In CHCl3, the fluorescence measurements revealed the formation of deprotonated D only in the D-B-A configuration for the (EA)4 peptide (Fig. ), whereas the (A)8 peptide did not show it (Fig. ). This finding already highlights the role of the solvent (polar MeOH vs. non-polar CHCl3) and of the peptide's structure in these solvents (see discussion below) the PT process. Since the steady-state fluorescence spectra provide only the stationary averaged state of the system, we conducted time-resolved fluorescence measurements. In water, the time-resolved measurements revealed the source for the different emission spectrum of the D in the D-B-A (Figure ) configuration compared to just the D in water (Figure ). They show (Figure