The microscopic observations and the flow cytometry analysis were performed using previously developed protocols with small modifications. Briefly, cells were seeded into 24well cell culture plates. After 24-48 hours, the culture medium was replaced with CO2independent medium (ThermoFisher, Cat. #18045088) containing 10% FBS and 5 mM Lglutamine (Sigma, Cat. #G7513). Microtubule inhibitors were added, and cells were incubated for 48 hours at 37 °C in a heating incubator chamber mounted on the Zeiss Cell Observer microscope (Carl Zeiss GmbH). Control cells received an equivalent amount of DMSO without inhibitors.