The reversibility of SOµC-Bdpy2 was also evaluated by incubating released microchips with 10 mM GSH solution (Figure ) and then incubating them in a 10 mM NEM solution (Figure ) while using the cell culture medium. Figure shows that after incubation in the NEM solution, the fluorescence of SOµC-Bdpy2 is recovered, indicating that, even though Bdpy2 has been immobilized on the surface of microchips, this molecule maintains its reversibility nature to detect GSH changes on the microchip surface even at different concentration of GSH (Figure ).