We have demonstrated that LUVs with a diameter of 100-200 nm can be charged in nano-electrospray from the water solution, transferred into the vacuum, and thermalized to a defined electric potential in the SELINA instrument. The combination of two wide m/z-range frequency scanned quadrupoles with the second working in the resolving regime facilitates the mass filtering of vesicles based on their m/z and resulted in the separation of a broad initial size distribution (with FWHM of 200 nm according to DLS) into multitude of LUVs distributions having only 20-30 nm FWHM. The charged LUVs were mass characterized by a single-pass charge detector operated in the current study already near its detection limit. In future investigations we will apply a new charge detector based on electrostatic trap with a significantly lower detection limit. It will enhance the precision of the mass measurement -and will allow us for instance to extend our study to smaller vesicles.