We first prepared a binary phosvitin/SDS model emulsion with a high-pressure homogenizer and captured a brightfield image showing the distribution of oil droplets (Figure ). We then determined the distribution of the fluorescently labeled phosvitin antibody using STORM microscopy and overlayed the bright field and super-resolved images (Figures and). The data show that phosvitin does not cover all droplets, indicating a competition between phosvitin and the SDS emulsifier at the interface. Interestingly, most phosvitin localizations at the interface visually appear to be homogeneously distributed (Figure ). To perform further quantitative analysis, we require all localizations to be attributed to individual droplets. To this end, we used the trained model from StarDist to obtain a segmentation mask of droplets that we overlayed with the super-resolved image (Figure ). StarDist attributes the localizations from overlapping droplet regions twice, once for each droplet. We note that this behavior does not affect the extracted RPD histogram from the localizations (Supplementary Figure ). We then calculated the experimental RPD for each droplet and plotted the sum of all distance-normalized distributions (Figure ). We calculated a Arel of 0.36 for the binary phosvitin/SDS model emulsion. Following our previous classification, the value indicates a partially heterogeneous distribution of phosvitin around the oil droplets. Furthermore, fitting the distribution gives the amplitude of the localization precision of 2.77, which follows the results with the relative peak values indicating partial heterogeneity.