We calculated the melting temperature 𝑇 ! , a quantitative indicator of the thermostability, in the SE binding for DNA (dSE) and RNA (rSE) and the RNA KL interaction for KL_Ori, KL_Mut1, and KL_Mut2 (Figure 2b). The SE binding was treated by MELTING5, free software aimed at predicting the melting temperature of nucleic-acid duplexes, and the KL interaction was calculated by oxDNA, another free software suite using coarse-grained models developed for capturing thermodynamics and conformational properties of nucleic acids. The buffer and calculation conditions prescribed in the simulations were invariant (See Supporting Information for the input conditions). We observed that in the SE binding, the SE cohesion of RNA showed a higher 𝑇 ! than that of DNA. This higher stability of rSEs relative to dSEs reiterates the widely reported observations that RNA is typically more stable than DNA, which has been associated with enhanced base-stacking interaction. In the KL interaction, not surprisingly, the oxDNA simulation yielded a decrease in 𝑇 ! in the order of KL_Ori > KL_Mu1 > KL_Mut2.