In this study, a novel amino-acid-based, sustainable organic compound-based nanozyme (OA nanozyme) has been introduced that exhibits a peroxidase-like catalytic activity. The advanced fabrication methodology provides enhanced feasibility of nanozyme in terms of its smaller physical dimensions with a designated surface charge to support sensing applications. The OA nanozyme exhibits decent enzyme-like catalytic performance (peroxidase-like activity) with nontoxicity within biological perspectives, suggesting not only its decent catalytic profile which is compatible with conventional nanozymes but also a better sustainable perspective with biocompatibility. The OA nanozyme-based customized molecule sensing system enables the detection of histamine, a target allergic biomolecule, selectively and within a short time with decent sensing performance (LOD: 21.37pgmL -1 ). It is also capable of detecting pseudo-real