The Discovery Hybrid Rheometer is employed to analyze the dynamic rheological properties of hydrogels, utilizing parallel plate geometry with an 8 mm diameter. Frequency sweep measurements are conducted within the linear viscoelastic region, at a fixed strain and a temperature of 37 °C, ranging from 0.1 to 100 rad s -1 . The NMR spectra confirmed the structure of GMI. Figure shows the 1 H spectrum with peaks at approximately 13 ppm and 7.1 ppm, which are attributed to the -O-H bond of the carboxylic acid and the -CH=CH-bond of the maleimide ring. In Figure (b), the 13 C spectrum displays peaks at 170.8 ppm and 135.3 ppm, corresponding to the carbonyl groups and double bond of the maleimide ring, respectively. Additionally, the carbonyl group peak of the carboxylic acid was detected at 169.3 ppm. Based on the 1H NMR spectrum shown in Figure , it has been confirmed that the 4arm-PEG-Mal structure is present. Specifically, the peak observed at 6.9 ppm has been identified as protons in the maleimide, while the peaks at 3.8-3.4 ppm are attributed to protons in the 4-arm PEG backbone. By comparing the integrals of the resonance signals at 6.9 ppm and 3.8-3.4 ppm, it is estimated that the degree of functionality of the 4-arm-PEG-Mal is approximately 90%. The chitosan-SH structure was confirmed using 1H NMR analysis, which revealed the presence of cysteamine in the molecule through the observation of peaks at δ = 1.95 ppm. The modification of chitosan was determined by using the resonance of the acetamido group of chitosan as an internal standard at δ = 1.15 ppm. Additionally, the peaks observed at 4.0-3.0 ppm were identified as the protons in the chitosan backbone. By comparing the integrals of the resonance signals at 1.95 ppm and 4.0-3.0 ppm, it was estimated that the degree of functionality of the chitosan-SH was around 70%.