Pooled liver microsomes from male mice were purchased from Xenotech. Compound incubations were performed in the presence of an NADPH-regenerating system (5 mM glucose-6-phosphate, 5 U/mL glucose-6-phosphate dehydrogenase, and 1 mM NADP + ). The mouse liver microsomes (1 mg/mL), the NADPH-regenerating system, and 4 mM MgCl2 × 6 H2O in 0.1 M Tris buffer (pH 7.4) were preincubated for 5 min at 37 °C and 750 rpm on a shaker. The reaction was started by adding the preheated compound. The reaction was at selected time points (0, 10, 20, 30, 60, and 120 min) by pipetting 50 µL of reaction mix into 100 μL ice cold internal standard (50 µM) in acetonitrile. The samples were mixed and centrifuged (1000 relative centrifugal force/4 °C/15 min). The supernatant was directly used for LC-MS analysis. All incubations were conducted in triplicate, and a limit of 1% organic solvent was not exceeded.