Countercurrent Fractionation of "Curcumin"-containing Dietary Supplements. In order to understand the chemical constituents more fully, chromatography was employed to distribute overlapping NMR signals into separate fractions. In a previous study, curcumin knock-out methodology was established using countercurrent separation (CCS) methodology. Specifically, centrifugal partition chromatography (CPC) was employed to fractionate the non-polar extract, using the two-phase solvent system composed of HDiMWat (3:7:7:3, hexanes-CH2Cl2-MeOHwater). The separation was performed in normal phase (ascending) mode, employing both elution and extrusion steps. The weight distribution of the resulting fractions (Figure ) was as follows: