Our experiments show that the new reaction cycle we introduced can be used to create active droplets. In future works, we envision functionalizing the droplets with catalysts and reagents so that they can control their fate. For example, a catalyst could inhibit or accelerate dephosphorylation. To demonstrate that such an approach is viable, we tested whether other components, besides the two peptides, could partition into the droplets. We monitored the partitioning by confocal microscopy of Cy3-labeled single and double-stranded DNA, singlestranded RNA, and polystyrene sulfonate. Both the single-stranded DNA and RNA partitioned well and homogeneously throughout the droplets. Excitingly, the double-stranded DNA and polystyrene sulfonate partitioned well and seemed to form microdomains of high concentration within the droplets.