After the incubation period, the diameter of the resulting inhibition zone in the bacterial growth was measured. Controls without films and control films with free carvacrol were also tested. Finally, the inoculated Petri dishes were employed to count the colony forming units (CFU) and log reduction value (LRV). For that, agar medium was aseptically removed from the Petri dishes and homogenized in a sterile BagPage with 100 mL of peptone water for 2 min with a Stomacher. Serial dilutions were made with peptone water and plated in Petri dishes with 15 mL of selective agar medium Brilliant Green Agar from Scharlab (Barcelona, Spain). Plates were incubated at 37 °C for 24h. Results were expressed as log CFU/mL. LRV was calculated by comparison between control sample and samples with film (LRV= (Control log CFU/mL)-(Film log CFU/mL). The experiment was carried out in triplicate.