Herein, we propose a non-invasive, highly sensitive electrochemical detection of salivary pH and enzymatic glucose sensors by introducing in-situ pH control to enhance the sensitivity and stability, by producing molecular oxygen in the vicinity of the interdigitated microelectrodes (IDE). Leveraging the multiplexing capability of our chip, we were able to develop these sensors simultaneously on a single platform. Figure provides schematic illustrations of our system, detailing the electro-polymerization surface modification process for glucose oxidase (GOx) immobilization on the IDEs, and depicting the sensing mechanism of the glucose sensor. The fabrication procedure is thoroughly described in the materials and methods section. We conducted materials and sensor characterization to verify electrode modifications and comprehend the surface morphologies at each step. Additionally, finite element analysis (FEA) simulations were employed in both buffer and artificial saliva mediums to optimize local pH for enhanced sensitivity of the glucose sensor. Furthermore, we utilized the developed glucose and pH sensors to assess glucose concentration and pH levels in calf saliva, aiding in the determination of BRD and diarrhoea by benchmarking the results against saliva samples harnessed from both sick and healthy calves.