Finally, we wanted to test the applicability of the workflow on loaded nanodiscs containing AcrB, a ~115 kDa transmembrane protein. We measured AcrB solubilized in POPC nanodiscs under optimized quench conditions and after data processing and peptide filtering, we could obtain 301 peptides and 82.7% sequence coverage (Figure ), which is higher than previously reported for AcrB in DDM proving the utility of the established online delipidation setup.