Cell imaging. B16-F10 cells were seeded in a confocal dish at an approximate concentration of 1×10 5 cells/mL and incubated in a humidified environment with 5% CO2 at 37 °C for 24 h. Subsequently, the culture medium was discarded, and fresh culture medium containing TPE-Py NPs, TPE-Pyα-CD NPs, and TPE-Pyα-CD+PTX-NB NPs (1.25 µM) was introduced and maintained at 37 °C with 5% CO2 for an additional 4 h. The AIEgens were excited using a wavelength of 561 nm, and their emission spectra were recorded within the range of 600~700 nm under confocal fluorescence microscopy.