Mangiferin was subjected to acetylation; a solution of mangiferin in pyridine was reacted with acetic anhydride with continuous stirring at 40 °C for 36 hours. Then the resulting reaction mixture was washed with water and extracted with chloroform soluble fraction then the resulting reaction mixture was kept for evaporation, which gave a residue that was further purified with the help of column chromatography, and the fraction was concentrated to provide the desired compound, acetyl mangiferin (3).