Gold-aptamer-nanoconstructs are gold nanoparticles aggregated by aptamers sensitive to a target nucleic acid sequence. When aptamers hybridise with target nucleic acid sequences, gold nanoparticles disaggregate initiating a signal transduction pathway (Figure ), which can be characterised via colourimetric, spectroscopic and lateral flow assays. Gold-aptamer- nanoconstructs sensitive to enteroviral nucleic acid were fabricated via a systematic methodology, which included identification of target nucleic acid and in silico aptamer design, synthesis of gold nanoparticles and functionalisation of their surfaces, and characterisation of the signal transduction pathway.