To image the hyperpolarized malate that formed from the metabolism, we applied OPE-s90 to selectively excite the malate protons, immediately followed by a 90° rf pulse to return the magnetization back to the z-axis, and then applied a fast low-angle shot (FLASH) sequence with centric reordering to acquire a 64x64 pixel image. The complete imaging sequence is shown in Fig. . The sequence was repeated every 12 s to acquire a train of images as the metabolism progressed. For comparison, we also show nonselective 1 H images acquired using the same FLASH sequence after the hyperpolarized signals had fully relaxed. The metabolites in the 10 mm tube are at such low concentration they are not visible in the thermal equilibrium images, but a large water background signal is present. The PHIP experiment was performed twice to acquire axial and sagittal images, and the first image from each acquisition train is shown in Fig. alongside the nonselective thermal equilibrium images. The train of images acquired in the axial-orientation experiment are shown in Fig. . The receiver gain was set to 101 for hyperpolarized experiments, and 1 for thermal equilibrium experiments, and this translates to a factor of 2 difference in SNR.