The PDMS stamps also showed durability and reusability, making them effective for up to 10 consecutive prints before needing to be replaced, referred to as 'aged stamps' (Figure ) To increase the efficiency of μCP, we tested different stamp treatment methods to prevent transferring PDMS residue and optimize the surface energy of the stamp. After evaluating fluorosilane (FS) treatment using chemical vapor deposition (CVD) , and (3-Aminopropyl) triethoxysilane (APTES) treatment, the untreated stamps proved to have better functionality and significantly higher SNRs (Figure ). The associated fluorescence images for untreated stamps compared to aged, FS treated, and APTES treated stamps are shown in Figure .