Unlike most oxidase, alcohol oxidase is highly specific for its reaction with O2 serving as oxidizing substrate. This distinctive feature is critical for preventing AOx from donating electrons to the classical electron mediators, such as ferrocendimethanol and Os-complex modified polymers, used in electrochemical biosensing. Therefore, the AOx-based oxygen scavenging system does not interfere with the electron transfer pathway responsible for signal detection. This makes the AOx-based oxygen scavenger generally deployable in electrochemical oxidase-based biosensors.