The explanation of specific and unspecific binding is also consistent with temporal evolutions of the amide bands. When IAV can bind specifically to GD1a, we observe biexponential kinetics with time constants of 3.2 and 55 min (Figure ), which could represent quick initial binding and previously observed mobility of IAV particles, respectively, until efficient multivalent IAV-GD1a interactions are accomplished. In the case when specific binding does not occur, the SEIRA signals increase monotonically with a much slower time constant of approximately 152 min (Figure ). Therefore, combining the data from Figure , we conclude that SEIRA successfully monitored IAV particles as they bind specifically to GD1a molecules on the free-floating bilayer lipid patches, but not on the ChEO3SH-supported parts of the tBLM.