The micellization behavior of the three conjugates was studied using pyrene as a fluorescent probe. The samples OA-Gly-OH, OA-Thr-OH, and OA-Phe-OH were selected for this investigation. A small volume of 5 mol/L solution of pyrene in acetone is prepared. Then around 5 uL solution is added to 0.6 M pH 10 Na2CO3 buffer to make the finale concentration of pyrene in the buffer around 5 × 10⁻⁷ M. Thena known amount of OA-Gly-OH, OA-Thr-OH, and OA-Phe-OH solid was dissolved in this buffer solution respectively to prepare the stock solution. The stock solution is diluted to different concentration with the buffer solution containing 5 × 10⁻⁷ M pyrene for fluorescence spectrum.. The fluorescence spectra of the solution were obtained at room temperature using a PerkinElmer LS-50B Luminescence Spectrophotometer. The fluorescence spectra were recorded between 350-500nm with excitation wavelength 334 nm. The slit width for both excitation and emission is set at 2.5 nm.