In order to identify the alkane phases during the melting and crystallization process, we acquired 2 H NMR spectra of C30d in bulk and in DMPC membranes in the relevant temperature range. Selected results are shown in figure . For bulk C30d, we can clearly distinguish the crystal, rotator and liquid phase. The crystalline signal features two sets of horns/shoulders, separated by approx. 34 and 120 kHz, as explained in the first part of this manuscript. The rotator phase resembles spectra of gel-phase lipids , while the liquid n-alkane results in a single peak. We obtain transition temperatures of about 60.0-60.5 • C and 62.5-64.0 • C for the crystal-to-rotator and rotator-to-liquid transition of bulk C30d upon heating, respectively. Upon cooling, the rotator-to-crystal transition temperature was between 60 and 58 • C. These results are in line with previous works and the DSC results that are described below (Figure ). In contrast to the bulk C30d case, it is more difficult to identify exact phase transition temperatures for the C30d/phospholipid mixture. Still, the observations match the 1 H NMR results shown in Figure : The intensity of the 2 H NMR spectra in the region between ±4kHz gradually increases from about 45 to 60 • C. In this temperature range, the spectral shape of the signal reveals the presence of alkane molecules with anisotropic motion in the membrane environment. Additionally, at 65 • C and above, a central, narrow peak becomes visible in the 2 H NMR spectra. Since the temperature range in which this peak is observed roughly matches the temperature interval in which bulk C30d is liquid, it seems reasonable to assign this narrow signal to phase-separated bulk alkane.