Such post-treatment strategy was found to (i) boost the enzyme loading (by securing the fixation of otherwise loosely attached TAs to the membrane surface), (ii) enhance the specific activity, and (iii) drastically curb the extent of enzyme leaching (Table , compare entry HeWT_HPAN3b and TsRTA_HPAN3b to HeWT_HPAN1b and TsRTA_HPAN1b, respectively). The surge in specific activity after treating with GA seems surprising, for crosslinking is known to rigidify the enzymes structure, and it is often argued to be the cause of partial deactivation (e.g. in cross-linked enzyme aggregates) . Yet the measurements were repeated (immobilization, activity assays, and Bradford tests to determine the loading) and the improvement was verified to be statistically significant (see the standard deviations in Table ).