Fractionation of the LNPs by centrifugation was done according to Chen et al. (2024). The large particles size fraction (LNP2-large) was obtained by centrifuging 5 mL of the LNP-2 dispersion (0.5 % w/v) for 1 min at 5000 rpm. The sedimented particles were separated from the supernatant by decanting and redispersed in 5 mL of H2O. Centrifugation and decanting were repeated 5 times. For the small particle size fraction (LNP2-small), the supernatant from the first centrifugation step was subjected to centrifugation for 10 min at 5000 rpm. Again, centrifugation and decanting were repeated six times. In the last step, the sedimented particles LNP2-small and LNP2-large were redispersed in 1 mL and 3 mL of H2O, respectively. Moreover, a mixture of both fractions (LNP2-mix) was prepared by combing equal parts of LNP2-small and LNP2-large.