The domain search method was employed to investigate the protein function of EBOV in this work. The results indicate that L, N, S, VP24, and VP35 have LCN type CS-α/β domains. S LCN type CS-α/β is a neurotoxin with a lengthy chain that can activate Na + channels. VP24, VP35, and N LCN type CS-α/β are short-chain toxins that inhibit voltage-dependent or Ca +2 -activated K + channels and partially activate sodium channels. This present study demonstrates that by utilizing an LCN type CS-α/β domain, S protein can activate Na+ channels and Na + /K + pumps in extracellular areas, promoting a high rate of Na + injection into the cell. N, L, VP24, and VP35 can regulate intracellular K + channels and Na + /K + pumps via LCN type CS-α/β domains, inhibiting K+ ion output. In Ebola patients, abnormal Na + channel and Na + /K + pump activity can result in hyponatremia; abnormal Na-Ca pumps can result in hypocalcemia; and abnormal K + channel inactivation might result in severe hypokalemia symptoms.