The acoustoinjection of microfluidic droplets is quantified using ImageJ analysis of high-speed camera videos from triplicate experiments. To investigate the impact of surfactant concentration on acoustoinjection, droplets are injected with actuation at 10 kHz, a low and high amplitude within the linear range of power, and 1, 3 and 5 wt% surfactant concentrations. The aspect ratios of droplets (n = 36) are measured before and after passing the injection region of the device to determine the percentage change in the aspect ratio of the injected populations. The percent change in droplet aspect ratios as a function of surfactant concentration are plotted using a frequency distribution plot with the coefficient of variation represented by error bars in MATLAB (Fig. ). This same measurement approach is repeated to investigate the impact of the inlet pressure rate for the injection fluid onto the pre-drop formation and therefore the injected volume into each passing droplet. The experiments were performed at 10 kHz frequency, input amplitudes of 0.5 Vp-p , 1.0 Vp-p and 1.5 Vp-p with 120 mbar and 135 mbar pressure at the fluid injection nozzle. The aspect ratios of droplets (n = 36) are measured before and after passing the injection region of the device to determine the percentage change in the aspect ratio of the injected population. The data are plotted using bubble chart in MATLAB to depict the injection fidelity of each device with the diameter of each point representing the coefficient of variation (Fig. ). For the determination of the injection efficiency, three highspeed-camera videos of 8 to 50 sequential passing droplets each are analyzed by counting the number of injected droplets.