One of the earliest qualitative applications was demonstrated by Sun et al. (2018), who employed the Single-probe SCMS to investigate intracellular metabolite changes in Scrippsiella trochoidea, a marine dinoflagellate, under various environmental conditions . Bulk filtration techniques are predominantly used to assess the physiological responses of microbial populations to environmental changes. The Single-probe SCMS technique provided profiles of intracellular metabolites in these single marine algae cells altered by different conditions such as light variation and nitrogen limitation. This work is a showcase of the potential applications of single-cell metabolomics studies of marine algae cells' responses to environmental stressors without extensive sample manipulation.