Samples were collected at two sites in the Pine Island Sound, Pine Island, FL (26.681361, -82.089000; 26.665222, -82.105278). Egg collars were collected and preserved in denatured alcohol. Ten egg collars were collected at both sites. Samples were stored at 4 °C until ready for use. A small section (500 -800 mg) of the egg collar was weighed, and DNA was extracted with the NucleoSpin Soil DNA Isolation Kit (Takara; Kusatsu, Shiga, Japan) as instructed. Extracted DNA (eDNA) concentration and quality was measured on a NanoDrop 2000 (ThermoFisher; Waltham, MA). DNA was stored at -20 °C until it was used.