The commercial standards that were used for the workflow validation were germicidins A and B, kanamycin, tetracycline hydrochloride, thiostrepton, globomycin, ampicillin and apramycin. The strain extracts that were used for benchmarking were derived from Streptomyces collinus Tü 365 (DSMZ 40733) that produces kirromycin and desferrioxamine B , Kutzneria sp. CA-103260 that produces epemicins A and B and Streptomyces sp. NBC 00162 that produces pyracrimycin A . 100% of all expected features were detected in the samples and SIRIUS accurately predicted 76 % of all formulas. CSI:FingerID accurately predicted approximately 62%