Adult wild-type AB strain zebrafish (Danio rerio) were housed in 10 L tanks in a fish-rearing system (Iwaki Aquatic Systems, Holliston, MA, USA). The fish were held in approximately 2:1 female to male cohorts at a density of 3-4 fish/L in buffered freshwater (475.5 mg/L Instant Ocean, 79.3 mg/L NaHCO3, and 53.8 mg/L CaSO4, pH 7.2.-7.5). The photoperiod was set to a 14:10 h light:dark cycle, and the water temperature was kept at 28.5 °C. The fish were fed twice daily, consisting of live brine shrimp (Artemia salina) in the morning and GEMMA Micro 300 micropellets (Skretting) in the afternoon. Freshly fertilized eggs were obtained by breeding multiple tanks. Viable embryos were collected, pooled, and maintained at 28-28.5 °C with a 14:10 lightdark cycle in egg water (60 µg/mL Instant Ocean) with a drop of methylene blue. The Woods Hole Oceanographic Institution Animal Care and Use Committee (Assurance D16-00381 from the NIH Office of Laboratory Animal Welfare) approved all experiments.