Previous research has focused on the use of flexible conductive polymers to wire the enzymes onto the electrodes, with common substrates including poly (3,4-ethylenedioxythiophene/polystyrene sulfonic acid) (PEDOT:PSS). Setti et al fabricated electrodes with PEDOT:PSS/GOx matrixes through thermal inkjet printing, which while promising, suffered from stability issues (e.g. PEDOT:PSS dissolves in electrolyte) and involved the redox mediator in solution, which cannot be practical in real applications. Yu et al recently realized a PEDOT:PSS hydrogel system that attaches glucose oxidase (GOx) within the network onto the carbon nanotube fiber modified electrodes. It demonstrated novel glucose sensing ability in the absence of additional redox mediators. However, the mechanistic details remains many conflicts and requires further studies. Generally speaking, conductive polymers offer several merits including flexibility, low-cost and adjustable properties. Meanwhile, confinement of the polymer at the electrode surface can be challenging, in addition to temperature and long-term stability issues. Biosourced lyotropic liquid crystals that are meta-stable over a range of temperatures in excess aqueous conditions show distinctive merits in enzyme. We recently reported the use of a redox-active lyotropic liquid-crystal structure for the effective immobilization of glucose oxidase (GOx). The system is based on a three-dimensional nanomaterial known as a lipid cubic phase (LCP), as shown in Figure . The lipid cubic phase is formed by type II lipids, organized into a bilayer with a unique triply periodic minimal surface (TPMS). This structure adopts a continuous labyrinth network structure, periodic in three spatial dimensions, with two non-intersecting aqueous channels, located on either side of the lipid membrane, as illustrated in Figure . The structure is naturally occurring and has been observed in various types of biological systems. 1-monoolein (MO) is one of the typical lipids that undergoes self-assembly transformation into cubic phase structures in excess aqueous conditions. Owing to its biocompatibility and ready availability, MO has been extensively used in protein crystallization , drug delivery and biosensing .