To obtain α-synuclein for the experiments, BL21 (DE3) E. Coli cultures, carrying the pT-7 plasmid encoding the WT human αsynuclein gene, were grown overnight. The culture was used to inoculate 1 L of LB-Amp media in a 3 L flask and was grown at 37 • C at 180 rpm shaking. When OD 600 = 0.8 was reached protein expression was induced by adding IPTG to a final concentration of 1 mM. The induced cells were incubated for 4 h at 37 • C at 180 rpm shaking. The cells were harvested by centrifugation at 4 • C, 7000 x g for 20 min and stored at -20 • C until further use. Bacterial pellets corresponding to 1 L of culture were resuspended in 20 mL of 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 with 1 mM PMSF. The suspension is sonicated with a probe sonicator for 2 min at 10 s intervals with 30 s pause at 40% amplitude. 1 µL Benzonase was added to the cell lysate and centrifuged at 4 • C, 20000 x g for 30 min. The supernatant is collected and the solution is boiled for 20 min. The solution was subsequently centrifuged at 4 • C, 20000 x g for 20 min to precipitate the heat-sensitive proteins, α-synuclein remaining in the supernatant. Next, 4 mL saturated (NH 4 ) 2 SO 4 was added for 1 ml supernatant to salt out α-synuclein. The solution was stirred at 4 • C for 15 min and centrifuged at 4 • C, 20000 x g for 20 min to pellet down the protein. The pellet is dissolved in 7 ml of 25 mM Tris-HCl pH 7.7 and 7 µL DTT is added to the final concentration of 1 mM. Next, the protein solution was dialyzed against the same buffer for 16-18 h at 4 • C with a change of the buffer after 12 h of dialysis. The dialyzed protein solution was then subjected to an anion exchange coloumn (AEC) (Hi-Trap Q Hp 5 ml, GE Healthcare) followed by size exclusion chromotagraphy (SEC) (HiLoad 16/600 Superdex 200 pg. column) and eluted in 10 mM of sodium phosphate buffer (pH 7.4). Protein concentrations were assessed by measurement of absorption spectroscopy at 280 nm (using a ProbeDrum instrument, Proba-tionLabs, Lund, Sweden) and the concentrations calculated using theoretical molar extinction coefficients predicted by ProtParam (Expasy, Switzerland).