The cIM 1-pass data also exhibited a marked increase in precursor-product matching across all assessed targets (Figure ). As precursor-product matching attributes are applied in DynamX as peptide threshold parameters, their increase results in greater proportions of peptides passing into the final peptide map. Owing to the DT-aligned nature of precursor-product matching in HDMS E , the observed increase is likely a consequence of higher mobility resolution in combination with the observed increase in sensitivity and chromatographic resolution. However, whilst the Cyclic IMS and Synapt G2-Si instruments both utilise collision induced dissociation (CID) for precursor fragmentation, it is important to note that the Cyclic IMS instrument also contains an updated collision cell design which could also contribute to this increase. Nevertheless, these results demonstrate the potential for Cyclic IMS with 1pass cIM routines to provide considerable improvements in data quality under identical HDX-MS conditions.