As expected from the theoretical analysis presented above, the best-fit model curve depicted in Figure is neither linear nor hyperbolic, as could be expected in the absence of tightbinding, but rather it has a characteristic "field-hockey stick" shape, with a distinct minimum appearing near [I] 0 ≈ [E] 0 = 0.01 µM. The slope of the nearly linear upward branch of the best-fit curve is equal to approximately 0.1 µM -1 s -1 , which is identical to the "true" value of k on . The intercept on the k obs axis is near k obs = 0.0017 s -1 , which is approximately equal to the best-fit value of k off + [E] 0 k on = 0.00011 + 0.0125 × 0.125 in appropriate units. to Eqn .