The tested subjects were: Subject A a healthy 25-year-old female, Subject B a healthy 30-year-old male, Subject C a healthy 25-year-old male, Subject D a healthy 28-year-old male and Subject E a healthy 30-year-old male. The results show that the subjects' PSA levels are within the normal healthy range for all male subjects. Very low PSA levels were measured for the female Subject A, lower than the lowest limit of the detection for the device, showed as the horizontal line, since normal PSA levels in healthy females are about 0.002 ng/ml For the in-vivo measurement, the device was connected to a handle support printed in a 3D printer to facilitate its precise, easy, and stable insertion. Figures and visually depict the skin pricking process before and during the insertion, respectively. Each microneedle on the device serves a crucial function in rupturing the capillary network creating individual blood pools. These blood pools subsequently fill the meticulously designed sensing area within the silica-protected window. Figure presents the distinct blood pools generated by each microneedle as a consequence of the skin pricking procedure, each pool corresponds to a specific microneedle on the device chip. The blood extraction-free microneedle sensing platform presented in this study suggests a groundbreaking advancement in medical diagnosis, especially in POC testing. The current medical diagnostics heavily rely on invasive, time-consuming, and expensive procedures involving extensive blood extraction and manipulation, performed by trained professionals. The proposed diagnostic platform presented here utilizes chemically-modified SiNPs-embedded microneedles, enabling intradermal penetration and quantitative sampling and detection of the desired biomarkers directly from capillary blood. This innovative POC paradigm offers minimally invasive, manipulation-free whole blood detection, while exhibiting high sensitivity, specificity, and fast detection turnover, with multiplex capabilities without the need for external power use.