An important factor when assessing screening methods is the diversity of the retrieved compounds. As SpaceHASTEN is based on the idea of chemical similarity, there could be a risk that retrieved compounds converge to a small set of scaffolds defined by the seeds and initial cycles. To investigate this, the virtual hits were analyzed based on their scaffolds (ScaffoldTree Level 1) and on compound level by clustering (see Materials and Methods for details). Table shows the number of ScaffoldTree Level 1 scaffolds and the obtained compound clusters: both are indicating that in all three cases diverse sets of compounds were retrieved from Enamine REALSpace.