The ICFR configuration makes use of the high temperature and pressure conditions in the cellulosic pretreatment of bagasse to disrupt the cells and release microbial oil, removing the need for energy and capital-intensive drying and extrusion of the cell mass (which are required in the ICF configuration). Although integrating oil recovery constitutes a process intensification by reducing the number of unit operations, the pretreatment reactor itself is energy and capitalintensive and results in comparable costs under the same assumptions for oil recovery (Figure ). The MBSP from all configurations were sensitive to microbial oil yield and microbial oil recovery, the latter of which was dampened (i.e., made less sensitive) through the burning of unrecovered oil for heat and power cogeneration. The cellulosic configurations (i.e., ICF and ICFR) produce larger quantities of microbial oil and exhibit greater sensitivity to oil recovery than the DC configuration. In addition to oil recovery and microbial yield, the productivity and the maximum achievable titer are also key technological drivers of sustainability (Figures and; SI). At the baseline case, which represented conservative assumptions for fermentation performance which have been achieved at lab-scale using oilcane hydrolysate, Although biodiesel production from microbial oil may not be financially lucrative under these assumptions, it results in greater yields and a lower carbon intensity than biodiesel from soybean oil. Even under uncertainty in potential operating days, microbial oil yields, and oil recoveries, this result holds true for 100 % of evaluated scenarios (Figure ). Specifically, the DC and ICF yields were 1780 [1310, 2360] and 2750 [2050, 3640] L•ha -1 •yr -1 , respectively, which is 2 to 6 times greater than the yield of biodiesel from soybean oil (617 L•ha -1 •yr -1 ). soybean oil, points above the horizontal grey line achieved higher biodiesel yields and points to the left of the vertical grey line had lower carbon intensities.