Trypanosoma brucei gambiense is prevalent in 24 countries across West and Central Africa, whereas Trypanosoma brucei rhodesiense is found in 13 countries within East and Southern Africa. The former accounts for approximately 92% of HAT cases, while the latter is responsible for the remaining 8% . An estimated 65 million people are at risk of contracting this disease, with between 100,000 and 300,000 new cases reported annually, primarily in Central Africa .