The CRISPR/Cas system can open a new window of possibilities for genetic diagnostics that integrates the ease of use and cost efficiency of isothermal amplification with the diagnostic accuracy of PCR for genotyping, detecting cancer mutations and mutations that confer resistance to antibiotics, antiviral medicines or cancer drugs. Additionally, CRISPR/Cas system can fulfill the ASSURED criteria (affordable, sensitive, specific, user-friendly, rapid, equipment-free, delivered) set by the World Health Organization 294 for infectious disease diagnostics.