Chemicals of emerging concerns (CECs) including pharmaceuticals, personal care products and pesticides have caused increasing concern for the freshwater environment over the past decades. They are found ubiquitously due to widespread usage in society and can have undesirable effects such as persistence and activity at low concentrations . The presence of CECs in surface waters can result in adverse effects in aquatic ecosystems, due to endocrine disrupting properties and other negative ecotoxicological effects . Further concerns arise from low level exposure of antimicrobial agents (AA) which can contribute to antimicrobial resistance (AMR) . Whilst CEC levels are often low and sublethal for tested aquatic organisms (pg L -1 -ng L -1 ), chronic exposure to complex mixtures of CECs are challenging to assess . Furthermore, pharmaceuticals can either be excreted unchanged (as the parent compound) or as human metabolites (derivative structures). Some metabolites are also produced via degradation or transformation of parent compounds in wastewater treatment plants or in water environments. Metabolites can have equal or higher toxicity than the parent compound and many metabolites are understudied in the environment, potentially due to costs or availability of analytical grade standards or they simply may not be known .