Here we test a new NanoSIMS-HR instrument for lateral resolution, depth resolution, limits of detection, stage reproducibility, and isotopic ratio reproducibility. Lateral resolution measurements were conducted on an aluminum sample containing silicon crystals, and representative images were collected for (1) microalgae and (2) plant roots colonized with arbuscular mycorrhizal fungi. Depth resolution measurements were conducted on a delta layer sample at 16, 8, and 4 keV impact energy. Limit of detection measurements were made on a phosphorus implant. Isotopic ratio data were collected on a Si wafer and a quartz standard.