Another variation of PCR is real-time PCR, also known as quantitative PCR or qPCR for short. This variation records the quantitation of the target DNA sequences with fluorescence while the PCR machine amplifies them. These machines include a standard thermocycler platform with an excitation source, a fluorescent-detecting camera, and external computer and software to process the collected fluorescent data . One of the most used fluorescence detection methods is the TaqMan PCR. TaqMan PCR uses a dye-labeled nucleic acid probe, which is labeled with two fluorescent moieties . These include a reporter and a quencher. When the two moieties are close to each other, the quencher absorbs the fluorescence released by the reporter; however, during the extending stage of PCR, the Taq polymerase cleaves the probe and separates the reporter and the quencher, thereby allowing the reporter to release the fluorescence. The computer software detects and records the increased fluorescence in the data. Overall, the ability to quantitate the amount of targeted DNA sequence makes real-time PCR highly accurate and useful for the coronavirus diagnosis.