Li et al. used cryogenic electron microscopy (cryo-EM) to stabilize the structure, resolve the atomic surface of ZIF-8, and investigate its interaction with guest CO2 molecules . They reported that imaging ZIF-8 under cryo temperature at 300 kV improves the damage tolerance by a factor of two compared to imaging at room temperature (RT). The effect of cryo temperature at lower accelerating voltage, such as 200 kV, on the damage tolerance and the critical dose is still unexplored.