Gentle Hydration. Films were dried down in 2 mL glass vials from Fisher. First, 200mL of chloroform was added to a glass vial. 50nmol of lipid in chloroform was taken from a master stock and added beneath the 200mL of chloroform in the vial such that the lipid mixture remained dissolved. The glass vial was then vortexed, bath sonicated and vortexed again for 30 seconds each. The film was then dried down under a stream of argon. The vial was then placed in a desiccator overnight to remove any residual solvent. Films were then rehydrated in 0.5 mL of submicron filtered 500mM sucrose and heated to 65 ˚C, above the melting temperature of DSPC (54.4 ˚C), for 15 hours.