Cell viability was assayed by live/dead staining with calcein green and ethidium homodimer (EtHD), respectively. Hydrogels were washed in PBS and incubated in live/dead staining solution (4 µM EtHD, 2 µM calcein) for 1 hour prior to confocal imaging. Live/dead cell count was quantified from max intensity projections spanning 50 µm of hydrogel using CellProfiler and normalized to cell viability in TEG-crosslinked hydrogels.