All LC-MS analyses with cIM technology were performed on a SELECT SERIES Cyclic IM QTOF instrument coupled to an M-class nanoACQUITY™ UPLC™ system and HDX manager (Waters Corporation, Wilmslow, UK). After proteolytic digestion, peptides underwent trapping/desalting using a BEH C18 VanGuard pre-column (Waters Corporation, Wilmslow, UK) at 100 μL/min for 3 min in mobile phase A (0.2% formic acid in HPLC grade H2O), followed by an 8 min UPLC separation at 40 μL/min using a 1 mm  100 mm BEH C18 analytical column (Waters Corporation, Wilmslow, UK) with an 8-55% gradient of mobile phase B (0.2% formic acid in HPLC grade acetonitrile). All trapping and chromatography were performed at 1°C to minimise deuterium/hydrogen back-exchange.