To explore for possible new ligands to F13L-mutants by DWBEL coevolution, the most abundant representative F13L mutants were included into the amino acid F13L sequence before being Alphafold modelled. The 2003 MPXV reference strain (F13L-1), the E353K clade IIb mutation of the 2022 outbreak (F13L0) and the representative pooled mutants coding for the most abundant and highest EC50 Tecovirimat-resistant mutations (F13L1 and F13L2) were all Alphafold modelled (Figure , labelled red solid circles).