For HPSEC analysis, 100 µL of the hydrolysate were mixed with 150 µL of 0.1 M lithium chloride solution (eluent). The analysis was carried out on a Hitachi system (Merck, Germany) equipped with a TSKgel G6000PWXL column (Tosoh Bioscience, Japan). 0.1 M lithium chloride was used as the eluent with an isocratic flow rate of 0.5 mL/min. The analysis was performed at a temperature of 60 °C and a refractive index detector (L-7490, Hitachi, Merck, Germany) was used for detection.