Liposomes, self-assembled spherical structures composed of a lipid bilayer, have attracted consid-24 erable attention in science, medicine, and industry due to their structural similarity to cells and 25 natural vesicles. Recently, cell-sized liposomes have emerged as versatile tools in various fields 26 from membrane science and Kawano, 2023) to cell mimicry . Their good bio-28 compatibility also positions them as the prime choice for being scaffold in creating synthetic cells 29 ) and as candidates for implanting vitro transcription-translation 30 (IVTT) systems in synthetic biology studies . With such a 31 wide range of valuable liposomes-related applications, various methods have been developed for 32 the bulk preparation of liposomes, such as thin-film hydration (Reeves, n.d.), electroformation 33 , and inverted emulsion transfer 34 . Though there are low requirements and easy implementation for liposome 35 preparation, they have limitations in achieving precise control over liposome size.