Proteins were extracted from the cell culture and quantified by microBCA assay. Briefly, media was removed, and cells were washed with cold DPBS. After removal of DPBS, 100 μL of lysis buffer (0.1% Triton X in TBS, containing complete Protease and Phosphatase inhibitors, Roche) was added to each well. Cells were detached by thoroughly scraping the bottom of the well plate for 5 min. The lysate was transferred to pre-chilled Eppendorfs pipetting up and down 15-times. Each sample was ultrasonicated for 5s prior to centrifugation at 12,500 rpm for 15 min at 4 °C. The supernatant collected for protein quantification using a microBCA assay (supplier) with a bovine serum albumin (BSA) standard curve (12.5 -100 μg/mL).