Desolvation method is used for the preparation of Curcumin nanoparticle. In this Chitosan and non-ionized surfactant 0.1% Tween 20 is dissolved in deionized water to create the base solution which is placed on a magnetic stirrer for uniform mixing. After which aqueous ethanol is added dropwise to the Chitosan solution under mixing at 70ºC. Nanoparticles are formed. The formed nanoparticles and suspension are centrifuged at 10000 rpm for 2 minutes. The collected centrifuge is again centrifuged at 15000 rpm for 15 minutes to remove excess of free Curcumin. The Curcumin nanoparticle precipitate is washed with 1 ml of desolving agent to remove free Curcumin and ethanol residual. The collected nanoparticles are resuspended in deionized water and freeze dried to obtain the Curcumin nanoparticle. The method involves straight forward steps like stirring, centrifugation and freeze drying making it reproducible. It is well suited for producing nanoparticles with controlled size. Washing and freeze drying ensures that the final nanoparticles are impurity free. However, the method could include a step to calculate the yield of nanoparticles which will help in ensuring minimal loss during centrifugation . Schematic diagram for preparation of curcumin nanoparticle by Desolvation Method is shown in figure . Among the various preparation methods, the combination of Freeze-dried Anti-solvent Crystallization (CRS-FD) followed by High Pressure Homogenization Method presents a novel and efficient technique for producing Curcumin nanoparticles. The method initiates with the formation of Curcumin nanoparticles through Anti-solvent Crystallization in which 1 gram of Curcumin is dissolved in 20 ml if Acetone, which serves as solvent. This solution is added dropwise to a larger volume (200 ml) of an aqueous anti-solvent that contains different concentrations of stabilizers such as Polyvinyl pyrrolidone (PVP) and Hydroxypropyl Methylcellulose (HPMC). This is an important step as the choice and the concentration of the stabilizer can significantly influence the size and stability of the nanoparticles formed. Thereafter, this resulted Curcumin-stabilizer suspension is subjected to stirring at 600 rpm at 25ºC before it is rapidly and instantly freeze dried at -70ºC for 48 hours. This step is crucial as it is a step vital for preserving the newly formed nanoparticles. The Freeze-drying process makes sure that the moisture content is reduced which enhances the stability and the shelf life of nanoparticles. Following the Freeze-drying process, the prepared nanoparticle suspension is subjected to High-Pressure Homogenization. This method consists of multiple stages of pressure adjustments. The initial pressure is set at 500 bar for the first five passes after which the suspension further processed at a final pressure of 1000 bar for an additional ten passes. The High-Pressure Homogenization facilitates the reduction of the size of Curcumin nanoparticles and enhances the overall stability of the nanoparticle.