Using interaction networks to predict engineering hotspots. Having established that the NMR determined hotspots can be optimized through computational stability design to obtain an exceptionally proficient Kemp eliminase, a logical question to ask is whether the process could have been performed fully computationally. That is: given the expansion of approaches to predict allosteric communication and residue interaction networks computationally, would a computational approach be able to predict dynamically important hotspots that could be targeted for further engineering effort?