A similar synthetic strategy was applied to Gem to demonstrate the versatility and robustness of our degradable polymer prodrug nanoparticle design. Functionalization of Gem was carried out by amidation of mono-2-methacryloyloxy ethyl succinate methacrylate using carbodiimide chemistry to produce GemMA. Its successful preparation, with an overall yield of 40 %, was confirmed by the appearance of vinylic (5.61 ppm) and methyl (1.8 ppm) protons, along those from the ethyl succinate moiety (2.5-2.7 and 4.3 ppm) on the 1 H-NMR spectrum (Figure ). In addition, the amide carbon bond was observed by rROPISA was carried out from the POEGMA 28 macro-CTA at 20 wt % solids with LMA (targeted DP n,PLMA = 150) and GemMA (f GemMA,0 = 0.1) in DMF at 90°C for 24 h (G1, Table ).