In conclusion, we have developed PUB, an operationally simple module for the detection of inorganic (pyro-)phosphate in biochemical assays. This method is cheap, versatile, and readily compatible with standard spectroscopic equipment and high-throughput experimentation on various scales. Ammonia vapor pressure Potential carbonate binding to phosphorylases Pyrophosphate hydrolysis thermodynamics GstIPP Solubility of magnesium pyrophosphates Differential scanning fluorimetry Protparam MMRT model Transition state theory Akaike's Information Criterion Enzymes with non-zero ∆ * Inflection point hypothesis Sugar phosphate stability Arsenolysis