From family 4, the azide uracil derivative 27 was neither active against the parasite nor toxic to host cells at both applied concentrations, presenting an activity profile like that of the uracil propargyl compound 15. Uracil conjugates 28-31 were non-toxic to HFF on both tested concentrations. Amide analogues 28, 29 and 31 presented reduced to no efficacy against T. gondii β-gal even at 1 µM.