We tested the hypothesis that moderate (sub-erosion) increases to flow and the related reductions in diffusion barriers could reduce or even eliminate ΔĪOS because overshoot is situated in the concentration-limited regime of the polarization curve. We limited the maximum flow rate to QT=10 mL h -1 because beyond this value, the returns on the MFC output are diminishing. In addition to an expected increase in Pmax and Īmax (Figure ), the increased flow rate significantly reduced the absolute overshoot to ΔĪOS=40 mA m -2 (with a corresponding overshoot factor of ΘOS=5%). Inspection of the corresponding polarization curve indicated a small reduction (5 kΩ) in the internal resistance over the value obtained at QT=1 mL h -1 (36 kΩ) (Supplementary Materials, Section S5). This was corroborated by calculation of the internal resistance (not shown). Previously, in a two-chamber G. sulfurreducens MFC with continuous anolyte replacement, higher internal resistances and lower power were measured at high flow rates. This may reflect amplified stresses, e.g., those due to flow-enhanced transport of low concentration O2 in solution to the EAB, as other literature examples typically show an increase to power with flow.