Consequently, the number of Bdpy1-GSH conjugates at the surface of microchips increases with time resulting in higher fluorescence intensity. The time-dependent ratiometric fluorescence is also shown in Figure (f), which results from the medium fluorescence intensity of about 200 microchips in different time intervals incubations. For SOµC-Bdpy2, the excitation and emission wavelengths were set at λex = 590 nm, λem = 595-630 nm and λex = 528 nm, λem = 530-580 nm for Bdpy2 and Bdpy2-GSH, respectively. As it can be seen in Figure , it is clear that there is an increase in fluorescent intensity at λem = 530-580 nm, which is associated with Bdpy2-GSH, from 1 h to 6 h. However, between 6 h to 12 h, there is no considerable change in fluorescent intensity in either for Bdpy2 or the Bdpy2-GSH. The ratiometric calculations (Figure (f)) also confirms the fluorescent images, leading to the conclusion that after 6 hours, the Bdpy2 molecules on the surface of microchips have reached an equilibrium state with GSH solution. By comparing the result of time dependent experiments for SOµC-Bdpy1 and SOµC-Bdpy2, as well as the ratiometric signal calculations, a comparable difference between the ratiometric values in the same time intervals is obvious which can be related to the irreversibility and reversibility nature of two different functionalized microchips which make reversible microchips more suitable for long-term detection of GSH.