Output skyline transition lists were directly imported into Skyline with minimum m/z 50 and maximum m/z 10,000. High resolution raw files were analysed with the following fullscan settings: 3 peaks for centroided mass analyser at 50 ppm mass accuracy. Low resolution raw files were analysed with the following full scan settings: 3 peaks for TOF mass analyser at 5000 resolution. Glycan precursors were filtered for quality, only including those with a minimum precursor isotopic dot product (idotp) of 0.9. The idotp value of 0.9 was empirically selected to remove poor quality MS1-matches (caused by monoisotopic peak misassignment, incorrect charge assignment, and poor signal to noise ratios) while preserving high-quality matches .