Compartmentalization is a crucial step in the formation of life. Many studies over the past decades have highlighted the role of non-biological amphiphilic compounds in forming vesicles as compartments for the origins of life. This self-assembly of simple amphiphiles into membrane structures (protocells) could have compartmentalized early biological processes on prebiotic Earth and allowed for the selection of more fit individuals. However, it is unlikely that only a single amphiphile type would be present in such an environment, as prebiotic synthesis produces mixtures of compounds. Therefore, we assume that the first cells would have self-assembled from a mixture of available compounds in the environment. Furthermore, this process would need to occur at low amphiphile concentrations that would have been present on early Earth. Despite progress on understanding the prebiotic synthesis of diverse amphiphiles, studies of protocell self-assembly have generally considered relatively simple (1-3 component) mixtures.