-**Outcome:** T9αH1 was confirmed to perform the C9 hydroxylation, another previously unidentified step in the pathway. -**Outcome:** Although the yield was low (approximately 50 ng per gram of dry weight), this achievement demonstrates the feasibility of producing paclitaxel precursors in a heterologous system. The advancements achieved in this paper represent a significant milestone in the field of natural product biosynthesis and metabolic engineering. By identifying previously unknown enzymes and successfully reconstructing the pathway in a heterologous host, the study opens new avenues for the sustainable and cost-effective production of paclitaxel. This work not only has implications for industrial biotechnology but also enhances our understanding of complex plant secondary metabolism. The paper titled "Optimization of the fermentation media, mathematical modeling, and enhancement of paclitaxel production by *Alternaria alternata* after elicitation with pectin" details several advancements in paclitaxel biosynthesis through the following high-level technical innovations: