Here, we prepared anionic NBD-liposomes with average diameter of 122 nm, whose lipid composition mimics the plasma membrane of mammalian cells (Figure ). AAO membrane used in this study was characterized by scanning electron microscopy (SEM). Average pore size was determined as 212 ± 37 nm (Figure and Table ). Other parameters such as porosity, surface area and pore volume were shown in the Supporting information. AAO membranes were incubated in NBD-liposome-containing PBS buffer solution for 18h with gentle agitation at room temperature. Figure shows the fluorescence intensity of the supernatant after incubation. The fluorescence from NBD-liposomes decreased by 18% compared to that of the liposomes in the buffer without incubation with AAO membranes. This indicates that liposomes were successfully incorporated into AAO membranes. It is noted that this immobilization was saturated under the present condition from the examination of the time course (Figure ).