We performed multiple repeats of non-equilibrium pulling followed by umbrella sampling to obtain PMF profiles for the dissociation of three protein-protein, one protein-peptide, and one protein-ligand complexes. To show that the results are independent of MD software, we performed simulations with GROMACS 2021.5 and 2022.4 versions and OpenMM. To demonstrate that the results do not depend on the force field used, we ran simulations with CHARMM36 and Amber ff99SB-ILDN force fields. To sample multiple pathways within one simulation, we performed multiple simulations with the accelerated weight histogram (AWH) method with GROMACS and the CHARMM36 force field. Here, we provide the details of the simulation systems, simulation parameters, and procedures for pulling, US, and analysis.