The lipid film was obtained by drying under nitrogen gas and then hydrated overnight at 4°C in PBS buffer (pH 7.4) for NBD-Liposomes and PBS buffer with 0.5 mg / mL of 4 kDa or 40 kDa Antonia Red-Dextrans for Dextran-containing liposomes, respectively. The vesicles were then prepared by manual extrusion through polycarbonate membranes (Avanti Polar lipids) with pore diameters of 30, 100 or 400 nm using Avanti mini-extruder (Avanti Polar lipids), where for the 30 nm pore membrane, the lipid suspension was subjected to five freeze-thaw cycles before extrusion. For Dextran-containing liposomes, extruded liposome suspension was additional centrifugated with Nanosep Omega 100K filtration tube (Pall, Port Washinton, NY, USA) at 3000 ×g at 4°C and washed with PBS for three times to remove unencapsulated dextran.