Stock standard placebo solution was prepared. Further, secondary stock solution was prepared from the stock. Moreover, three different concentrations (90μg/mL, 100μg/mL, 110μg/mL) were prepared from the secondary stock solution. While the Dilution is carried out with 0.1 N HCl. The placebo is essential to account for any interference with excipient and it helps to establish a baseline for accuracy. The chosen concentration levels (90%, 100%, and 110%