Histology, Immunochemistry, and Goblet Cell Staining. Livers were fixed in formalin and embedded in paraffin. Samples were then sectioned and stained with hematoxylin-eosin to evaluate liver damage further. The extent of necrosis was determined by measuring the necrotic area relative to the area of the entire histological section utilizing the NIH ImageJ software and reported as a percent necrotic area. For IHC, antigen retrieval was performed by heating the sections in 10 mM sodium citrate buffer. Sections were blocked with 10% FBS and incubated with MPO primary antibody (R&D System, Catalog No: AF3667) overnight at 4°C. After incubation with biotinylated anti-goat IgG secondary antibody, sections were incubated with ABC reagent (Vector #PK-7100) followed by incubation with 3,3-diaminobenzidine tetrahydrochloride (DAB, Vector #SK-4100). The slides were then counterstained with hematoxylin and imaged with an optical microscope. Intestinal Goblet cells were stained by Alcian blue and counterstained by Nuclear Fast Red using Alcian Blue (pH 2.5) Stain Kit (Vector #H-3501) according to the manufacturer's protocol.