Regarding the protein-RNA complex refinement we adopted the MD input files prepared in our previous work . In summary, AMBER14SB FF with parmbsc1 63 parameters and the TIP3P water model were used to build the topology. To preserve the protein-RNA interface, we introduce harmonic biases on the distances between the phenylalanine residues and bases involved in non-bonded interactions; furthermore, we also added a restraining potential on the secondary structures of the protein, following the same procedure described by Kooshapur et al. . The metainference simulations were performed for 4.5 ns with and without the solvation layer correction activated, using 35 selected SAXS intensities with 𝑞 values between 0.008 Å -1 and 0.3 Å -1 as restraints.