Previous studies utilizing targeted methods focusing on phenolic acids have been applied to some of the tested species . In the work at hand, phenolic acids were also prevalent, and in similar concentrations as described previously. Besides phenolic acids, the other polyphenol classes have not been investigated as thoroughly by LC-MS. One study investigated catechins in king trumpet mushrooms , but these polyphenols were not detected here, which could be due to the non-optimal nature of the workflow for this polyphenol class. Untargeted methods were previously applied to mushrooms to study the general composition or changes, e.g. during browning .