Also between different central structures, the variability among CD spectra is very large (Fig. ), reflecting differences in their secondary structure. Most spectra are characterized by a pronounced negative band at 190-200 nm and scattered positive band(s) between 205 and 220 nm. The helical cluster 11 also shows the typical negative shoulder between 210 and 230 nm (Fig. and Fig. in SI). Remarkably, if we average the CD spectra of these twenty structures weighted by their REST population, we obtain a very similar result to the average of the entire ensemble of 2000 conformers, although the negative intensity of the main minimum at around 200 nm is slightly overestimated (thick blue line in Fig. ).