Once the plants were exposed to AgNPs, remarkable changes were noted in the morphology of plants, commonly used parameters for assessing the phytotoxicity of AgNPs in plants are growth potential, seed germination, root growth and reduced biomass leaf area. Many plant species were been tested to study to Phytochemical nature of AgNPs. One of which is Arabidopsis thaliana also known as Mouse Ear Cress. In a literature Arabidopsis thaliana was exposed to AgNPs of size 41 nm at a concentration of 100-500 mg/L, this resulted in reduced root length, leaf expansion and photosynthesis efficiency and disruption of plasma membrane's K + efflux and Ca 2+ influx, it also resulted in induced ROS accumulation . In another experiment conducted Arabidopsis thaliana was exposed to 0.2 µgL concentration of AgNPs of size 10,20,40 and 80 nm which caused inhibition of development of root hair and repressed the transcriptional responses to microbial pathogen which results in increased bacterial colonization . Arabidopsis thalian when exposed to concentration of 75-300 µgL -1 showed prolonged vegetative and shortened reproducible growth. It also decreased germination rate of offspring . In a study of Lolium multiflorum plant, the plant was exposed to the concentration of 1-40 mgL -1 of AgNPs of size 6nm, root biomass decreased from 18.6±1.3 mg to 4.7±0.7 mg also the root length decreased from 7±0.6 cm to 0.7±0.08 cm [53].