Modeling the decay of radiotracer concentration, however, revealed that imaging could only be performed up to 65 minutes after EOS, limiting 1h dynamic imaging acquisitions to 1 or 2 mice (Fig. ). Further improvement to radiotracer concentration by alteration of the Hypercarb cartridge (76 MBq/mL; Table , Entry 3) substantially increased preclinical output. In combination with improvements to the reaction RCY, the radiotracer concentration was increased to 102 MBq/mL. Imaging could now be performed up to 255 min after EOS (Fig. ), facilitating imaging of up to five mice using a standard small animal imaging bed, or 20 mice using the 4-bed mouse 'hotel' (Table , Entry 4).