Gum Arabic was selected as it is a branched hetero-polysaccharide containing protein and carbohydrate groups. It has been used to act as a biopolymer for removal of pesticides in polluted water bodies. Gum Arabic has been used for green chemical synthesis of silver nano particles. During this process, Gum Arabic acts as a reducing agent to reduce silver ions, then Gum Arabic forms a complex with Ag atoms, subsequently forming Ag-GA NPs. In this work, an electrochemical deposition method was used to fabricate Ag-GA SERS substrates. The size and morphology of the silver nanoparticles was tailored by controlling electrochemical deposition parameters such as deposition time, deposition voltage, and the precursor concentration. During the electrochemical deposition process, silver ions are electrochemically reduced, then combine with Gum Arabic monomers to form a Ag-GA nanoclusters complex, which deposit on the chip surface in the vicinity of the electrodes. The electrochemical approach greatly accelerated Ag +  Ag process compared to the natural reducing process by the Gum Arabic alone. In addition, tailoring deposition parameters, enables not only the size and morphology of the Ag-GA nanoclusters, but also the regular distributed gap pattern in between the nanoclusters, to be engineered.