Generally, all the Wssue slices were prepared in a similar way. Before the secWoning the Wssues were removed from the liquid nitrogen and were kept at -15°C in a cryomicrotome chamber (Cryotome FSE, Thermo Fisher ScienWfic, Chesire, UK) for 1 hour before cuvng. Thin Wssue slices (12 µm) were placed on indium-Wn-oxide (ITO) InteliSlide glasses (Bruker Daltonics, Bremen, Germany) and immediately thaw-mounted. Awer cuvng, the Wssue secWons were vacuum dried for 45 min. Awer preparaWon, the glasses were held in a laboratory freezer at -80°C (NU9483, NUAire Inc. Plymouth, MN, USA) in hermeWc boxes. Before each analysis glasses were moved to a vacuum desiccator and kept there for 45 min to prevent moisture condensaWon. Awer drying, the opWcal image (600 dpi) of the InteliSlide glasses with dried Wssues were taken. Awer analysis, Wssue secWons form the nervous Wssue were stained with cresyl violet.