The column was filled with the stationary phase of the gradient elution system and equilibrated with the initial mobile phase at 10 ml/min and 1600 rpm. The crude extract (3.81 g) was dissolved in 3 ml of lower phase + 3 ml of upper phase and injected into the CPC column through a 20 ml loop. As described in Table, the mobile phase was pumped in descending mode for 110 minutes. 3. The column was extruded by switching the mode selection valve for 15 minutes at 20 ml/min. Fractions of 20 ml were collected over the whole experiment (elution and extrusion) and combined according to their thin layer chromatography profiles (TLC).