ART in animals has been applied (i) in agriculture and livestock production, to improve genetic traits, such as milk yield, growth rate, and disease resistance, enhancing productivity and sustainability in livestock industries; (ii) in conservation biology, to address challenges in endangered species conservation by preserving genetic material, supporting captive breeding, and combating inbreeding depression; (iii) cross-species surrogacy has emerged as a strategy to sustain populations of species with critically low numbers. It has been applied also in companion animal breeding, to supports breeding programs for pets, particularly for rare or exotic species, ensuring genetic health and diversity.