A second "recovery" experiment using 2NapFF-Na at a concentration of 40 mg/mL was also created with an aim to establish the effects of combined stirring and resting on viscosity. Four lots of 9 mL of 2NapFF solution at a concentration of 40 mg/mL were prepared using the quantities in Section 1.3. Solutions were made in 50 mL Falcon tubes, stirred with the same (25 x 8 mm) stirrer bars, and wrapped in Parafilm. Solutions were either stirred at 400 rpm for one day; stirred at 400 rpm for seven days; stirred at 1000 rpm for 1 day; or stirred at 1000 rpm for seven days. After these initial different stir periods, viscosity was measured. Solutions were then left undisturbed for a seven-day period. Viscosity measurements were then taken after this seven-day rest period. The pH was adjusted to pH 10.5 ± 0.1 using 0.1M, 1 M and 2M NaOH; or 1 M and 2 M HCl as required for each solution before each viscosity measurement was performed.