Cooling at cryogenic temperature improves the critical dose of {431} and {110} Bragg plane by a factor of 1.5 and 1.6 as compared to room temperature measurement at 300 kV. The improvement at 200 kV is 1.1 and 1.4 times for {431} and {110} Bragg planes. Lowering the temperature effectively enhances dose tolerance as it mitigates the impact of sample heating. By reducing the diffusion of secondary radicals generated during electron beam interaction, cooling helps minimize the underlying damage mechanism . The formation of thick ice crystals can induce sample drift and reduce signal-to-noise during imaging . On the other hand, increasing the temperature to 125°C decreased the critical dose of {431} and {110} Bragg plane by a factor of 0.64 and 0.73, respectively, compared to RT measurement at 300 kV. The contribution from the higher lattice vibration at elevated temperatures promotes bond breaking and leads to a reduction in the critical dose . No significant effect of sample drying in a vacuum was observed.