Preliminary experiments to find the best excitation frequencies via attraction forces were performed with 10.29 ± 1.01 µm fluorescence polystyrene particles (FSEG008, Bangs Laboratories). When strong resonances were found, yeast cells, i.e., Saccharomyces cerevisiae, bought from a local supermarket, were submerged in de-ionised water and injected into the microfluidic channel to be used as tracer particles for the acoustic streaming. Given the limited visual accessibility of the out-of-plane vortices, experimental evaluation relied on secondary in-plane streaming visualized by the yeast cell circulation near the embedded microbubbles.