We used a similar procedure and final concentrations of polymer and DNA to prepare polyplex solutions for binding affinity experiments involving UV-Vis absorption spectroscopy. By varying the volumes of polymer stock solutions added to DNA stock, we created polyplex formulations with N/P ratio ranging from 0.1-10. Samples became slightly turbid upon adding polymer stock solutions to DNA stocks, indicating polyplex formation. Additionally, we prepared solutions of only polymer, without DNA, with similar final polymer concentrations as the polyplex samples. All samples were then incubated at room temperature for 24 hours to equilibrate prior to performing binding affinity experiments.