Enhanced sampling methods can thus be used to overcome this limitation. Broadly speaking, enhanced sampling methods used to investigate nucleation from multicomponent liquid phases can be classified depending on whether they introduce a bias potential as a perturbation of the system's Hamiltonian -such as metadynamics and umbrella sampling -or are based on efficiently sampling the space of reactive paths using techniques such as Transition Path, Transition Interface, and Forward Flux Sampling, or the construction of Markov State Models from unbiased trajectories. Biased enhanced sampling methods typically aim to estimate the free energy barrier associated with the nucleation and to discover nucleation pathways. Nucleation rates are usually obtained by complementing the free energy barrier information with estimates of the rate prefactor, often carried out using approaches similar to those adopted in the seeding method. A direct calculation of nucleation rates is only practical in simple cases .