In all nine samples, 401 features were annotated with the described workflow (Table .8). As polyphenols are a molecular class rich in isomers, establishing structural annotation confidence is not straightforward. Therefore, the annotation of the relevant features was split into different confidence levels adapted from Schymanski et al. and described in detail by Oesterle et al. . Of the 401 features, 39 were identified with reference standards (level 1) and 81 were annotated by spectral libraries (level 2a). An additional 163 features had a spectral library match, but it was unknown which isomer was the correct structure (level 3a). Utilizing in silico fragmentation, an additional six features with a known structure were annotated (level 3b) and 112 features with the isomeric structure unknown (level 3c). A heatmap was generated to show all the features following log10 normalization (Fig. A.1). Moreover, 170 (42%) of the features were conjugated with one or multiple sugar moieties contributing to the difficulty in identifying the correct isomeric structure (Table .8). Following feature annotation, the polyphenol class of each feature (Fig. ) was determined resulting in 221 (55%) flavonoids and 180 (45%) non-flavonoids overall.