The coacervation was performed in two different processes. In general, when performed in solution, a peptide solution at was added to achieve the desired concentration into a buffer solution (25 mM) with the desired ionic strength using NaCl. The buffers used were acetate buffer (pH 4.8), phosphate (pH 7.4), and carbonate (pH 10.5). For the coacervation during evaporation, the peptide solution was added to the desired buffer solution (with 0.3 M NaCl). Triply distilled water was added when necessary to achieve the same concentrations between samples. Droplets (2 µL) were deposited on clean glass slides (76 mm × 26 mm × 1 mm, Paul Marienfeld GmbH & CO. KG, Lauda-Königshofen, Germany) and viewed using light microscopy (Axio Scope A1 fluorescence microscope, Zeiss, Oberkochen, Germany). The glass slides were cleaned before use by treating with UV/ozone (Jelight, Irvine, California, United States) for 10 min, submerging in SDS (2%, 30 min), and treating with O2 plasma (Atto, Diener Electronic, Ebhausen, Germany) for 1 min. All measurements for the phase diagrams were performed in triplicate.