To perform Raman mapping, we placed 5 l of the oocyst suspension in water between two 0.25 mm thick quartz coverslips (Alfa Aesar), separated by a 1 mm PDMS ring. We then find a settled oocyst and take sequential measurements of the Raman spectrum starting from the top left and moving in 1 m steps using the piezo stage (PI-P5613CD, Physik Instruments), for a final mapping area of 5 m by 4 m (30 pixels per oocyst). We performed two accumulations at each location to cover the same spectral range of 600 -1780 cm -1 as in other measurements. We measured on five individual oocysts.