Lastly, we demonstrated that this highly fluorogenic aggregate can be applied for bioorthogonal fluorogenic imaging in living cells: by controlling the BCN location, we can observe turn-on signals in specific cellular environments such as mitochondria, cellular membranes, and lipid droplets. Considering recent advances in bioorthogonal chemistry to non-genetically label biological systems with clickable handles, this fluorogenic aggregate will become a key tool to visualize various molecular events inside living systems.