The authors measured the degree of denaturation of T4 bacteriophage with a constant concentration in solutions of different denaturants at constant temperature T = 73 • C. Each of these solutions has four components: a denaturant (1), 0.05 M Tris buffer (2), 0.001M EDTA (3), and water (w). The expression 35 can be written accordingly as L 1 +L 2 +L 3 +L w .