Concurrently, take tetrabutylammonium fluoride (TBAF, 1.0M in THF, Millipore Sigma, 216143-50ML) out of the fridge and place in the fume hood to warm it to room temperature. Once the oligonucleotide has completely dried, place TBAF under argon using a sterile needle through the septum, and flash each syringe 2x with argon before use. Add 500 ul TBAF quickly to each tube and mix briefly. Put tubes in a jar containing dessicant (Drierite, Fisher Scientific, 07-578-3A), flash bottle with argon and parafilm shut. Place jar on shaker platform at room temperature for 36 hours.