All analysis was performed on a Shimadzu LCMS9030 liquid chromatography-quadrupoletime-of-flight mass spectrometer (LC-QTOF-MS) equipped with a Nexera XR LC system and a standard ESI source. Detailed chromatographic conditions and acquisition settings are reported in Table . For chromatography, a Waters Atlantis Premier BEH C18 AX (2.5 μm, 2.1x100mm) column with pre-column (VanGuard FIT Cartridge, 2.5 µm, 2.1x5mm, containing the same stationary phase) for LC analysis was acquired from Waters (Tampa, USA). Mobile phase A was a 5 mM ammonium bicarbonate in water (pH 6.9, adjusted with acetic acid) and mobile phase B was a 5 mM ammonium bicarbonate in acetonitrile:ultrapure water 9:1 (pH 8.9, adjusted with diethylamine). The flow rate and injection volume were 0.4 mL/min and 5 µL respectively.