Interestingly, the propensity of α-helices along the N-terminal Asn6-to-Asp16 is comparatively low compared with those of the other two regions, which becomes negligible in the case of CHARMM22* PTMetaD-WTE (Fig. ) and unbiased simulation (Fig. ), suggesting that DHH1N simulated under CHARMM22* demonstrates negligible probability to form α-helices in the Asn-rich N-terminal domain.