For instance, a salt bridge was formed between a SNAC molecule and R36 in semaglutide, which (Table and Fig. ) indicate that the viscosity-corrected hydrodynamic radii for SNAC and semaglutide increase for both compounds when mixed together. This result is consistent with the computationally observed co-aggregation of the two compounds. Notably, the 1 H DOSY NMR signals for SNAC exhibit (Fig. and Table ) two diffusion coefficients, suggesting that SNAC aggregates of at least two different sizes are formed. Overall, these combined experimental and computational observations provide a direct indication for aggregation between SNAC and semaglutide in the aqueous layer to (1) help monomerize the semaglutide in aqueous solution 6 and (2) improve the affinity of the semaglutide-SNAC aggregates to membranebound SNAC aggregates (which we hypothesize helps recruit the semaglutide to the membrane surface as discussed in detail below). See Supplementary Movie 7 for a visual depiction of this process.