Phalloidin-FITC (Thermo Fisher, USA) staining of actin filaments followed by Hoechst 33258 (1 mg/mL; Merck, USA) staining of cell nuclei was performed in SH-SY5Y cells. Cells were cultured in 200 μL of cell culture medium on cell culture chamber slides at a density of 2×10 4 cells per well. After seeding, the culture medium was replaced by 200 μL of tested solutions, and the cells were treated for 6-48 h. The cells were fixed with 3.7% formaldehyde for 5 min at 37 °C. The cells were permeabilized with 0.1% Triton X-100 for 15 min at 37 °C. Then, phalloidin-FITC (1 μM) was incubated with the cells for 30 min at 37 °C to visualize actin filaments. Hoechst 33258 was used to visualize cell nuclei at a final solution of 2 μg/mL. After 10 min of incubation, the cells were washed two times with Dulbecco's phosphate buffer (DPBS; pH 7; 1 mM). The actin filaments (FITC filter, 480/30 nm) and cell nuclei (DAPI filter, 375/28 nm) were visualized with an Eclipse 80i fluorescence microscope (Nikon, Japan).