We performed alamarBlue® cytotoxicity assays to investigate whether compounds design to be an antifouling layer have toxic effect for HeLa cells. This standard cellular assay allow for cell proliferation and cytotoxicity effects study. In the living cells, after intracellular uptake, due to the response of cellular metabolic reduction, resazurin is reduced to the fluorescent resorufin, allowing for read based on adsorbance or fluorescent.