The conventional 1 H NMR spectrum and DNN-PS 1D 1 H NMR spectra of kanamycin (K) and glucose (G) mixtures recorded at different ratios. Because both of these compounds are saccharide-based, the spectral complexity due to severe overlapping of scalar coupling multiplets is substantial in the conventional spectrum (blue). On the one hand, the DNN-PS spectrum eliminates such overlaps and resolves all chemical shifts. Resonances marked with a cross and filled circle, respectively, are used to calculate the concentrations of glucose and kanamycin. (b) The peak intensities obtained from the conventional (blue), DNN-PS (red and the error bars are the predicted uncertainties), and PSYCHE (green), are integrated and presented against sample concentrations (kanamycin (filled circles) and glucose (cross)). Pearson correlation coefficients, r, for PSYCHE and DNN-PS range from 0.987 to 1.000, which is ideal for quantitative measurements; on the other hand, for glucose resonance, from conventional, the measured 'r' value is found to be negative due to the severe signal overlapping with kanamycin resonance. Therefore, when the resonances are severely overlapped, the conventional experiments cannot be used for quantitation. Another measure of the performance is the normalized RMSD, 𝜌 " = % 𝑡 !"# /𝑡 !"#,"%&' RMSD/slope, which normalizes the RMSD both with respect to raw intensities and with respect to the time required for the experiment, tacq, relative to the conventional 1D, tacq,conv. (c) The applicability of the DNN-PS scheme is demonstrated along the direct data acquisition dimensions of a 2D -1 H-1 H TOCSY acquired on this mixture. The conventional 2D-TOCSY has significant cross-peak overlaps; however, all peaks are resolved in the DNN-PS processed spectrum. Projections also show improved spectral resolution and sensitivity for the DNN-PS TOCSY when compared with the conventional TOCSY. The threshold for the conventional TOCSY is set 4 times lower than the DNN-PS TOCSY.