Liver tissue containing spontaneous metastases was collected, sectioned, embedded in OCT media and transferred to storage at -80C. Experiments were approved by and conducted in accordance with the National Health and Medical Research Council Statement on Animal Experimentation, the requirements of New South Wales State Government legislation, and with the rules for animal experimentation of the Biological Testing Facility of the Garvan Institute and the Victor Chang Cardiac Research Institute (protocol #18/12).