In this study, we focused on porous anodized aluminum oxide (AAO) as a platform for the integration with anionic liposomes. AAO is a well-characterized porous material with highly uniform, ordered and liner pore produced by anodization of aluminum in aqueous acid electrolytes. Their pore size can be easily regulated in the range of 10 -400 nm by tuning anodization conditions such as electrolyte species, pH and applying time. These structural features and ease of production render AAO useful for immobilization of drugs and biomolecules into its pore and controlling the release from the pore. We sought that AAO should be a useful platform as their pore size well matches commonly used lipid vesicles for liposome drugs and model of extracellular vesicles. Besides, it was reported that synthetic liposomes could intactly adsorption on solid surface of some metal oxide substrates including Al2O3 in proper conditions. Here, we used a commercially available AAO membrane with approximately 200 nm pore and examined its interactions with synthetic anionic liposomes that were labelled with fluorophores based on spectrometric and microscopic techniques. We demonstrated that liposomes were immobilized into AAO pores with no leakage for a week. We also found that the liposomes were rapidly and on-demand released from AAO pores by ultrasonic irradiation with not only keeping the size distribution and the morphology of the liposomes but also cargo remaining (Figure ). We also discussed the molecular interactions between the liposomes and AAO membranes for the development of the AAO-based materials integrated with liposomes. (Alabaster, AL, USA). Antonia Red-dextrans (4 kDa or 40 kDa) were purchased from Sigma-Aldrich (Darmstadt, Germany). Porous anodized aluminum oxide (AAO) membrane was purchased from Cytiva (Tokyo, Japan). The alumina membrane was rinsed with MilliQ water, methanol, acetone, and MilliQ water in that order before use, followed by ultrasonication in MilliQ water. After rinsed again with MilliQ water, the membrane was finally dried in an oven at 50°C. Other reagents were commercially available and used without further purification.