We obtained the crystal structures of ORF3a (PDBID: 6xdc) proteins from the Protein Data Bank. The domains such as malaria parasite antigens were then annotated (Figure ). The TryThrA_C A of ORF3a is shown in Figure .A in the transmembrane alpha-helix and beta folding region. TryThrA_C B denotes the beta fold region on the membrane's surface. EBA-175 VI A overlaps with TryThrA_C A, near the transmembrane's inner side. Thiol_cytolys C of ORF3a is in the transmembrane region and overlaps with EBA-175 VI A, as illustrated in Figure .B. The Thiol_cytolys_C region contains domains Band_3_cyto and 4.1m. Via the TryThrA_C A and TryThrA_C B domains, ORF3a invades the erythrocyte. ORF3a binds to erythrocyte membrane glycoprotein A via the transmembrane EBA-175 antigen, as illustrated in Figure . Simultaneously, the Band_3_cyto and 4.1m domains are anchored to the 4.1 protein of red blood cell membrane. Finally, Thiol_cytolys_C establishes calcium ion efflux channels on the membrane of red blood cells.