This observation is in good accordance with literature data where a high specificity of luciferases towards ATP was described. However, we also observed the ). The impact of adenosine 5'monophosphate (AMP) was studied in more detail as inhibitory effects on luciferases were described before. To mimic the conditions of the activity assay, mixtures consisting of different AMP to ATP ratios were analyzed with a total concentration of 400 µM (Figure ). For all combinations, no significant influence on the luminescence signal was observed (Table , Figure ). Hence, autoluminescence and luciferase inhibition was excluded for all tested (deoxy)nucleosides and (deoxy)NMPs.