Over the past thirty years, molecular simulations have become an integral and routine part of understanding biological processes at the molecular level. Advances in both CPU and GPU hardware, and more reliable and sustainable software packages, have led to both wider uptake and greater numbers of applications across multiple fields. Microsecond-length simulations are now routine, allowing exploration of biomolecular dynamics on timescales relevant for a variety of biological processes, whether through conventional simulation, enhanced sampling, or kinetic modeling of the underlying process.