We further use low-voltage TEM to observe the self-assembly morphology of three compounds when the concentration set at 100 mM which is high above the CAC of the three conjugates. The images confirmed the formation of higher order self-assembly structures. For all the three compounds, both particles and fibers are formed. The fiber structures which usually not present in fatty acid assemblies indicate the important role that amide bond playing in the formation of the disctince fibrous structures. Notably, OA-Phe-OH showed a stronger propensity for forming more fibers but less particles. This might be due to π-π interactions also contribute to the formation of fiberous structures. These interactions may enhance molecular stacking and contribute to the observed fiber formation. Despite this distinction, all three compounds OA-Gly-OH, OA-Thr-OH, and OA-Phe-OH exhibited the ability to form both particle and fiber structures under similar conditions.