Samples were left to equilibrate overnight as is described in detail by Chang et. al., to allow the insoluble unencapsulated curcumin to aggregate and precipitate. Samples were then centrifuged at 13k r.c.f. for 10 minutes each, the supernatant (containing LLC particles) removed carefully, and the curcumin pellet redissolved in ethanol. The supernatant was analysed via DLS. Each particle population was determined to have a hydrodynamic diameter of around 250 nm. Redissolved curcumin pellets were measured for absorbance and compared to a standard curve (SI Fig. ). % Curcumin encapsulation (given as % loading efficiency) was then calculated using the difference in concentration before and after LLC formation. % loading efficiencies for monoolein based cubosomes and phytantriol based hexosomes were around 50 %, with phytantriol cubosomes slightly lower at around 40 % (Fig. ). No appreciable difference in DLS-derived size distribution was seen for MHF samples before and after equilibration, with PDI values below 0.2 (Fig. )