Reagent generation was performed by ChemPartner using their in-house procedures. Briefly, the therapeutic antibody antigen-binding fragment (Fab) in Freund's Adjuvant was used to immunize 5 BALB/c and 5 SJL mice with 3 immunizations per mouse done once every two weeks. Complete Freund's Adjuvant was used for the initial injection, followed by boosting with incomplete Freund's Adjuvant. Serum antibody titers were determined for each mouse by ELISA with the Fab of the therapeutic being bound to the plate as a capture and a goat anti-mouse IgG-HRP (Sigma, catalog #A0170) for detection. The mice with the highest titers were chosen to generate hybridomas as described previously . Hybridomas were grown for 14 days and supernatant was collected for screening.