For characterization and rapid assessment of transfection efficiency of synthesized OM-pBAEs, a reporter plasmid encoding the green fluorescent protein (pGFP) was selected and condensed with OM-pBAEs to form polyplexes. The hydrodynamic diameter of the resulting polyplexes ranged from 175 to 260 nm, similar to the original formulation, with a polydispersity index (PDI) of 0.2 to 0.6 (Figure ) as measured by dynamic light scattering (DLS). All polyplexes had a dimension and size distribution compatible with known cell entry mechanisms and in vivo delivery standards .