Similarly, under our experimental conditions, neither enzymatic nor spontaneous hydrolysis was observed for the lactone moiety. While the approach was less efficient with acetic acid compared to vinyl acetate as the acyl donor, it could still be optimized for the synthesis of other DHLc esters with carboxylic acids in the future. This might be achieved by increasing the quantity of molecular sieves or employing alternative water removal systems, such as a Dean-Stark apparatus, which would be compatible with the solvent mixture used and its boiling point.