All oxPUFA. As described above, the molecular networking analysis of individual oxidized PUFA is capable of isolating cluster of family of NEO-PUFAs, with the potential of further annotating remaining series. We have established a feature-based molecular network incorporating all the analyzed oxidized PUFAs through the alignment of the four previously used feature tables (mimicking real samples), allowing us to establish new connections between series of different PUFA precursors from the same families. For instance, (Figure , right corner), automatic dereplication using the NEO-MSMS library highlighted the cluster comprising the E-or D-type NEO-EPA (thanks to the identification of PGE3, or its likely isoprostanoid diastereoisomer at m/z 349.2021, orange nodes), 9-E1-PhytoP derivatives (from ALA of m/z 325.202, violet nodes), as well as E-or D-type DHA derivatives (m/z 375.2177, blue nodes) and A-or J-type EPA metabolites (m/z 331.1915, orange nodes). It is then possible to annotate some of the detected E4-and A3-IsoPs based on their MS/MS fragmentations, as 4-E4-NeuroP and 17-E4-NeuroP (Supplementary Information Figure and). Furthermore, 15-A3-IsoP was easily identified as directly connected to PGE3 (almost identical fragmentation pattern, Supplementary Information Figure ) .