High resolution mass spectrometry analysis yielded > 3000 peaks (Table ). In terms of distribution between CHO, CHON and CHOS containing formulas, the sample was somewhere between the IHSS reference materials SRFA and PLFA. TRM-0522 contained less N and S containing peaks than PLFA (which are actually the most numerous in that sample), but was much more N and S rich than SRFA (Figure ). However, it should be noted that multiple N and S containing peaks, as well as CHONS peaks, could not be confidently assigned following Orbitrap analysis , and so the true diversity of the TRM-0522 sample could not be completely assessed .