In summary, we present profiles of destabilized proteomes in response to cellular exposure to three chloroacetanilide herbicides. While some proteins are destabilized by each treatment, the overall profiles from each herbicide exposure are unique. About 70% of targeted proteins are known to be subject to haloacetamide conjugation at cysteine, consistent with adducts being the primary mechanism of destabilization, but the extent of destabilization does not correlate with haloacetamides reactivity, reflecting the distinction between conjugation and stability. Hsp40 affinity profiling is an effective assay for determining the effect of environmental toxicants on the cellular proteome, both distinct from and complementary to existing technologies.