The substantial disparities in environments between bacterial and mammalian cells 54 might present an obstacle to the design and construction of artificial droplets. Meanwhile, we have developed amphiphilic peptides that self-assemble into amyloid-like fibrils in cells; sfGFP fusion with Yn peptides consisting of YEYK repeats resulted in dynamically arrested gel-like aggregates . In this study, by changing the sequence from YEYK repeats to YKYK repeats to weaken the peptide interaction, we succeeded in fabricating droplets intracellularly.