With this in mind, a supersystem HF-3c correction has been applied to all MBE(n) calculations for the large inhibitors, with results listed in Table . These calculations use the full enzyme although MBE(n) calculations are applied with R cut = 8 Å rather than the systemspecific cutoffs used in Table . The number of subsystems generally doubles for every 2 Å that is added to R cut ; for example, in the case of 1BOZ at the MBE(3) level we obtain 22,001 subsystems for R cut = 8 Å, 45,433 subsystems for R cut = 10 Å, and 88,811 subsystems for R cut = 12 Å. To perform MBE(3) at the ωB97X-V/def2ma-QZVP level requires ∼ 1.1 × 10 6 h of computer time to complete for the largest of these systems, 1BOZ.