While the intensity appears to correlate with the concentration of the adduct, for this method to be applicable for quantification we must calibrate the measurement, i.e. establish a correlation between intensity and concentration under a known condition. The most rigorous approach is either to inject a known concentration of the isolated solution of adduct into the cell and measure the emission intensity or to upgrade the setup with a downstream CO2 sensor and flow meter and a reference electrode. This allows for measuring the potential of the negative half-cell against a reference under a known condition, creating a standard concentration-intensity calibration plot. In this proof of concept, we make an assumption that operating the microfluidic cell under a pure stream of CO2 (1 bar) from the beginning of the charging process facilitates adduct formation and results in the full dominance of BTMAPAQ(CO2)2 2-adduct, consistent with our thermodynamic view. Under such an assumption, the fully charged capacity of 37 C measured by the potentiostat is attributed to the production of the adduct, which can be correlated with the 6000 units of intensity measured by the microscope in channel 2. Utilizing this established calibration, we then conducted an experiment involving a charging phase under a pure nitrogen stream, followed by purging the system with a pure stream of CO2 to capture carbon dioxide for only a short wait time afterwards. It is anticipated that the resulting intensity of the adduct, and hence the concentration, would be lower than observed in the first case (Figure ). Indeed, the concentration of the adduct formed under these conditions, extracted from the intensity of channel 2, is measured at 0.035 M, representing approximately 70% of the total capacity. The method is equally applicable for the detection of adducts under dilute streams of CO2. Figure illustrates the data extracted from the operation of the microfluidic cell under dilute streams of 1% and 400 ppm (100% included for comparison). We observe the formation of 0.025 M of the adduct under the dilute condition of 0.01 bar CO2 (1% CO2, 99% N2) within 50 minutes, detected in real-time during the formation. This short timeframe (50 minutes) appears insufficient for any adduct to form under the 400 ppm condition. Moreover, the method is robust enough to be employed for the extraction of kinetic rates of adduct formation chemical reactions. To achieve this, we increased the imaging rate to sub-second intervals (125 ms) and conducted experiments using 1,5BTMAPAQ and 1,8BTMAPAQ isomers. Through CV analysis, we demonstrated a kinetic rate constant for the 1,5BTMAPAQ isomer almost three times that of the 1,8BTMAPAQ isomer (Table ). The in situ fast imaging of the adduct formation for the two isomers similarly reveals a threefold difference in kinetic rates.