It should be noted that the plasma treatment temporarily renders the microfluidic device chan-107 nels hydrophilic. Therefore, it is recommended to allow the device to rest overnight, enabling the 108 channels to return to a hydrophobic state before conducting experiments (Deshpande, Birnie, and 109 Dekker, 2017). This resting period ensures optimal performance and prevents any unintended 110 effects due to temporary hydrophilicity.