Mechanocatalytic partial depolymerization is proven to be a robust and reliable method for the depolymerization of cellulose and different lignocellulosic feedstocks towards glycans. Testing different acid contents of sulfuric acid as well as different milling durations lead to a feasible partial depolymerization with an acid content of 6 %. Milling durations were best at 85 min and 170 min depending on the composition of the feedstock. It could be shown that pretreatment by extraction increased the yield of the total soluble fraction up to 40 % and additionally lead to a molecular weight distribution of the desired range 1-10 kDa. In comparison to glycans from hot water extraction process (pilot plant) the molecular weight of precipitated glycans was reduced to 1/3 and the polydispersity narrowed down from 2.9 to 1.4. With this, a molecular weight of 13.0 kDa respectively 12.1 kDa was achieved increasing the yield from 5 % to nearly 10 %. This makes the approach by mechnocatalytic partial depolymerization very promising for increasing the yield and reaching the molecular weight range in one step. To further assess and improve the energy demand/product-yield-ratio a scale-up of the process is planned. It could be shown that mechanocatalytic partial depolymerization is a robust and reproducible method to obtain lignocellulose-based glycans as sustainable biobased chemicals.