Additionally, compound 26 displayed promising antibacterial activity against different strains of the Gram-positive bacteria Streptococcus pyogenes (Str. pyogenes) and S. aureus with MIC values of equal to or less than 3.13 μg/mL, while its 1'-O-methylated derivative showed weaker antibacterial activity as it was only active against one strain of Str. pyogenes with MIC values of 6.25 μg/mL with no activity against the other strain of Str. pyogenes or any strain of S. aureus up to a concentration of 12.5 μg/mL , indicating that O-methylation at position 1 greatly affected the antibacterial activity of averantin .