The chemistry experimental part, with full description of experimental procedures and characterization data for all compounds are presented in the Supporting information. In a primary screening, each compound was evaluated at two concentrations 0.1 and 1 µM and added to the media prior to the infection as previously described . Control non-infected non treated HFFs cultures or T. gondii β-gal infected but not-treated cultures were cultivated in complete medium containing 0.01 or 0.1 % DMSO. 96-well plates were incubated for 72 hours at 37˚C/5% CO2 as previously described .