FMOPhore scan yet appeared in the Hotspot-category on the Dy-FMOPhore mode. In both modes we highlight the percentage of interactions divided into bond types between the probes and each residue, e.g., Tyr-A-264 can form hydrophobic, pi-pi stacking and hydrogen bond, (Figure ). Also, including the Dy-FMOPhore mode can significantly improve the 3D-FMOPhore model generation, this is observed in the difference of the 3Dcloud of vectors between the closed and open states of the P-loop.