Researchers increasingly favour this method due to its high sensitivity, specificity, real-time monitoring capability, and ease of use. Additionally, this method is versatile and can be used across a variety of tissues and cellular levels, covering different components of plant seeds. However, it does have inherent limitations that must be carefully considered and addressed. Traditional coplanar dyes mostly experience the concentration quenching effect which means the fluorescence will be reduced when the dye concentration increases to a certain extent. Therefore, these dyes may result false-negative signal of ROS concentration continuously increases to a certain extent.