In summary, a highly bright NIR-II luminescent nano-contrast agent based on an AIEgen DIPT-ICF has been rationally designed and prepared. Compared to ICG, DIPT-ICF nano contrast agent holds the merits of high brightness, superior photostability, long-circulating time, and excellent biocompatibility, which could fulfill the requirement of an ideal contrast agent in complex physiological environment for organ transplantation surgery. The results indicate that a single dose of DIPT-ICF NPs contrast agent could monitor the entire surgical process very clearly via luminescence due to its high brightness and long-circulating character. This is much better than traditional luminescent contrast agent of ICG, whose luminescence brightness and circulating time are not sufficient to support the completion of a whole organ transplantation surgery. In livingdonor nephrectomy, NIR-II angiography of renal vasculature based on DIPT-ICF NPs helps surgeons visualize the renal vascular branches and their anatomical location to avoid iatrogenic donor kidney injury. Meanwhile, the surgical complications in vascular and ureterovesical anastomosis can be observed in real-time by NIR-II angiography and ureterography using DIPT-ICF NPs as a contrast agent. Furthermore, GFB injury of the donor's kidney following warm ischemia and renal graft reperfusion could be evaluated through DIPT-ICF NPs based NIR-II imaging. The present study demonstrates a superior NIR-II nano contrast agent and its application in monitoring the whole process of organ transplantation surgery, which provides reliable and effective technical support for monitoring and evaluating renal transplantation in the clinic.