In conclusion, ZnO NPs were successfully synthesized with the use of Camellia Sinensis var. Assamica is found in commercial tea dust, available in commercial "Lipton® black tea decaffeinated" tea bags. The FE-SEM analysis showcased the clusters of ZnO NPs, which were recorded in the presence of other organic impurities formed during the synthesis. This is reflected in the EDX elemental analysis, revealing the primary presence of Zinc and Oxygen, along with Carbon, likely originating from residual phytochemicals post-synthesis, indicating the need for thorough rinsing before preparing the nanofluid for antibacterial analysis. Furthermore, TEM revealed that the biosynthesized ZnO nanoparticles, mainly 40-60 nm in size, exhibited an agglomerated, sponge-like asymmetrical shape. The UV-VIS spectrophotometer analysis showed an absorption peak for the biosynthesized ZnO nanoparticles at 380 nm, shifted from the bulk ZnO absorption edge at 400 nm, indicating a nanosize effect. Peaks for carbon impurities, detected between 180-260 nm, exhibited lower absorbance compared to the ZnO nanoparticles. The antibacterial activity of for the ZnO nanofluid was tested against three gram-positive (Enterococcus faecalis, Methicillin-resistant Staphylococcus aureus, Staphylococcus saprophyticus) and two gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), after 24 hours of incubation at 37°C, which is the average internal body temperature of a healthy human. Staphylococcus saprophyticus and Pseudomonas aeruginosa showed the largest zones of inhibition (ZOI) at 8mm, while the lowest ZOI (6mm) was recorded for Methicillin-resistant Staphylococcus aureus and Escherichia coli. The results were compared to general antibiotics such as Ciprofloxacin, Vancomycin, and Meropenem, and seemed to perform relatively decently.