The peak current and potential position for both redox peaks were calculated in Origin Pro. Firstly, the data was split into two sections; the oxidation peak data, which ranged from -0.2 to 0.4 V, and the reduction peak data from 0.4 to -0.2 V (the second part of the potential cycle). Each section of the cyclic voltammogram was then plotted and the background was subtracted. To background subtract, a 4 th order polynomial fit was selected, and nodes were placed along the cyclic voltammogram to act as anchors. The region of interest for Cyt c, between -0.1 V and 0.2 V, was left clear of nodes in both the oxidation and reduction data sets, allowing a background to be created in this region. The interpolation method was changed from line to Spline and the background was then subtracted. The resulting data was plotted in GraphPad Prism 9, and the area under the graph function was used to obtain the peak height and position. This was repeated for the oxidation and reduction peaks for the scan rate of each sample.