The threshold for each of the genes was set at 300 RFU (relative fluorescence units) for S, ORF, and IC (RNase P) genes and 400 RFU for the N gene. For the test to be valid, the Ct (cycle threshold) value of the PC had to be less than 27 for each of the genes, while the Ct value of the NC had to be greater than 37 or N/A for each of the genes. If this case was not true, the test had to be redone due to possible contamination or machine error during the experiment. Each individual Ct value for the target genes of all the samples are illustrated in figure 2. Based on the Ct results, it is apparent that this test is valid because the values for PC and NC are within the range of the threshold. The values of the test sample demonstrate that it is negative from the virus because the RFUs for S-gene, ORF8, and N-gene did not exceed the threshold within the determined Ct cycles. Likewise for future tests, the sample can be categorized as either positive, negative, or inconclusive based on the values. The threshold and the determining Ct cycles may differ depending on the device and the manufacturing company.