In summary, we employed the VIEC technique to monitor the differential dynamics of chemical cargo release from liposomes loaded with two different electroactive neurotransmitters (DA and 5-HT). Liposomes were used to simulate bio-vesicles without a dense core or proteins. A finite element simulation with Monte Carlo optimization was adopted to estimate the kinetic parameters of chemical desorption and suggests that the rate constant of DA desorbing from the lipids inside the vesicle Is smaller than that for 5-HT. The slower desorption alters the release rate as recognized by a mode of best fit (SigExp or DblExp) for spike signal decay. Our models suggest this might be induced by a stronger interaction between cargo and vesicle membrane lipids, as more hydrogen bonding between DA and membrane lipid molecules can occur than for 5-HT. Hence, the existence of adsorption-desorption behaviour for transmitter molecules and the lipid membrane could be a basic component of the vesicular release process. Although the dense core might also play a crucial role in regulating chemical release from the cells having vesicles containing protein dense cores, regulation via adsorption-desorption from the vesicle wall can occur in all biological vesicles with or without a dense core. A better understanding of the interaction between transmitters and the vesicle membrane might provide strategies to regulate neurotransmitter, hormone, protein or drug release related to cellular communication, intracellular vesicular transport, and controlled delivery of liposomal drugs. EXPERIMENTAL DETAILS Chemicals 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, >99%), 1,2-dioleoyl-sn-glycero-3phosphethanolamine (DOPE, >99%) and cholesterol (ovine wool, >98%) were purchased from Avanti Polar Lipids, USA. Serotonin hydrochloride (≥98.0%), dopamine hydrochloride (98.0%), chloroform (≥99.9%), methanol (≥99.9%) were obtained from Sigma-Aldrich. All aqueous solutions were prepared using 18 MΩ•cm -1 water from Purelab Classic purification system (ELGA, Sweden).