From the time-course data, compound 14a was able to induce rapid degradation, with >50% CRBN levels relative to control depleted already after 1 h; maximal degradation > 80% was attained after 8 h (Figure ). The 14a-induced degradation of CRBN was found to be even faster in HEK293, with >80% protein already depleted after 1 h, and 98% degradation achieved after 8 h (Figure ). Once again the compound displayed selectivity for CRBN, as there was no appreciable VHL degradation at 1 µM over the time points tested in either cell line (Figure and Figure ).