Vaccinia virus is a prototypical poxvirus that enters cells in a pH-dependent manner and can induce transient macropinocytosis, endocytosis, and membrane fusion-mediated cytoplasmic penetration . Large VACV MV particle phagocytosis is dependent on actin dynamics and cell signaling . Through macropinocytosis, filamentous influenza virus enter cells as intact filaments and are transported to the acidic late endosomal compartment . Ebola virus is internalized into host cells via macropinocytosis in a manner dependent on viral glycoproteins . Macropinocytosis is the nonspecific, large-scale uptake of fluid by cells . Before the viral core is released into the cytoplasm, late endosome and lysosome markers are present .