In this study, we have demonstrated a new approach to the external control of transmembrane Cl - transport using dynamic covalent chemistry with azines. Firstly, we have successfully demonstrated that dynamic azine metathesis can be performed inside lipid membranes. This chemistry was used to induce transmembrane Cl -transport upon the addi7on of azines as an external s7mulus. An ac7ve Cl - transporter was synthesised in situ inside the liposomal membrane from an inac7ve pre-transporter. We demonstrated that it is possible to use more complicated mixtures of building blocks or to tune the rate of anion transport by the structure of the azine added. We also showed that pH can be used as an addi7onal s7mulus to ac7vate the dynamic covalent chemistry in the membrane. We have thus shown that, in addi7on to pH, redox and light, dynamic covalent chemistry can be used as a strategy to control transmembrane transport processes. In the future, this feature may be used in drug delivery systems and smart therapeu7cs.