show the liposome size distributions, as determined via dynamic light scattering (DLS). The sizes of the liposomes prepared using the microfluidic and hydration methods are 20 and 160 nm, respectively. The liposomes prepared using the microfluidic device are theoretically smaller and should display unilamellar structures. Conversely, the large liposomes prepared via hydration form multilamellar structures rather than single unilamellar structures. To confirm the liposome structures, we performed TEM, and Figures ) show the TEM images of the liposomes. The results of TEM correspond to those of DLS, and the liposomes form different structures, depending on their sizes and the preparation method. The liposomes prepared using the microfluidic device exhibit 20 nm-sized unilamellar structures, and the 160 nm-sized liposomes prepared via hydration display multilamellar structures. We used these liposome sizes to validate the microfluidic SAXS measurement system.