We substituted the hardly reproducible manual injection with a controlled one, leveraging an automatic syringe pump to perfuse the deuterium peroxide. We selected a range of flow rates to represent common ones used for continuous media recirculation in microfluidic devices, going up to the maximum allowed by our pump. As previously done, we placed a single scaffold in each well of the microfluidic chip used and injected D 2 O at different flow rates for each experiment. As represented in Fig. , in all cases, the water proton signal was removed from the scaffold region and pushed to the top of the well. Also, the low variability in the box plots for the post-injection indicates that the water proton signal was displaced to the top of the well (T ROI) for all the wells in every experiment. Hence, based on these results, we again validated the efficacy of MRI for studying perfusion and also demonstrated the advantage of the cryogel used for fast, efficient and consistent perfusion of media at a wide range of flow rates.