Metabolomics has enabled the identification of specific metabolites involved in signaling and communication during plant-beneficial microbe interactions . These metabolites play crucial roles in the recognition and establishment of symbiotic associations, as well as in the regulation of plant responses . For instance, strigolactones, a group of plant hormones, have been shown to act as signaling molecules that facilitate the recognition and colonization of plant roots by AMF . Gargallo-Garriga et al. ( ) used LC-MS to identify changes in root exudate metabolites, particularly flavonoids and their derivatives, during the symbiosis between the legume Medicago truncatula and the arbuscular mycorrhizal fungus Rhizophagus irregularis to study signaling metabolites in the soybean-Bradyrhizobium symbiosis. . The authors identified several flavonoids, such as genistein and daidzein, that were secreted by soybean roots and acted as chemoattractants and inducers of nodulation genes in the bacterial symbiont. Similarly, a study by used LC-MS to identify the signaling metabolites involved in the mycorrhizal symbiosis between the legume Medicago truncatula and the arbuscular mycorrhizal fungus Glomus intraradices . The authors identified several strigolactones, a class of plant hormones, that were exuded by the roots and stimulated the germination and branching of fungal hyphae, facilitating the establishment of the symbiotic association.