All cryo-EM experiments were performed on a Thermo Fisher Titan Krios G3i TEM operated at 300 kV and equipped with an autoloading mechanism located at Stanford SLAC cryo-EM center. Cryo-EM imaging data were acquired by SerialEM with a Gatan K3 direct-detection camera in the electron-counting mode with the Dose-Fractionation function. Electron diffraction patterns were acquired by EPU-D with a CetaD camera.