Recognizing that Cy3 range fluorophores appear best suited for in situ sensing applications targeting the selected food products, we aimed to investigate the visibility of fNAPs conjugated with such labels. To this end, an aminated 5-Carboxytetramethylrhodamine (TAMRA)-labelled fNAP construct was employed. While fluorescently labelled, single-stranded oligonucleotides can be visualized with relative ease given the abundance of fluorophores, fNAP visualization is complicated by the presence of associated quenchers. Confirming that such functional molecules can be visualized when exposed to food backgrounds is key in oligonucleotide-mediated sensing.