The dECM electrospinning solution was made by combining two solutions: Solution 1 consisted of 20% w/v polycaprolactone (PCL) with an average molecular weight 80,000 (440744, MilliporeSigma, St. Louis, MO, USA) in 2,2,2-trifluoroethanol (TFE) (A10788, Thermo Fisher, Pittsburgh, PA, USA). Solution 2 consisted of 20% w/v pepsinated dECM powder in TFE + 5% acetic acid (AC42322, Fisher Scientific, Pittsburgh, PA, USA). 1.5 mL of Solution 2 was pipetted into 1.5mL of Solution 1 to produce 3.0 mL of the dECM electrospinning solution (10% w/v PCL in TFE, 10% w/v dECM in TFE + 2.5% acetic acid). Electrospinning was performed on the TL-Pro-BM electrospinning machine (Tong Li, Shenzhen, China). As shown in Figure in the Supplementary Information (SI), a 50 mm wide metal mandrel was covered by a polystyrene (PS) sheet with 14.5 mm diameter circular holes cut with a Hobby 5S laser cutter (Full Spectrum Laser, Las Vegas, NV, USA). The circular holes defined the nanofiber deposition locations. A 5 mL Leur-Lok syringe (30964, BD, MA, USA) was used to pump the electrospinning solution through a 24 G metal blunt needle placed 17 cm from the surface of the mandrel. The syringe pump was set to a flow rate of 0.25 mL/hr.