Mirror-image life, which was first proposed by Louis Pasteur in 1860 refers to the creation of an artificial biosystem with all macromolecules presented in their opposite enantiomeric forms. In these self-replicating systems, L-nucleic acids serve to store genetic information creating D-protein workforce for biological function following the mirror-image central dogma. The de novo design of living entities has gained significant attention because of our fundamental interest in understanding the origin of life. Figure . Recent advances in realizing mirror-image synthetic biology using Dprotein enantiomers. Representative structures for illustrative purposes as D-ASFV pol X (PDB: 1JQE), D-Dpo4 (PDB: 3PR4), D-Pfu (PDB: 2JGU), D-LigA (PDB:2Q2T), L5, L18 and L25 (PDB: 4YBB) constructed in PYMOL.