Life cycle impact assessment of biodiesel production for Indian conditions from Jatropha curcas as feedstock shows that the biodiesel (with considering allocation) can reduce global warming potential by as much as 64.6% in GWP impact category; However, AP, EP, and HTP impacts can increase by 36.5%, 61.3%, and 52.5% respectively. Environmental impacts could be further minimized if the environmental impact intensity of Indian power grid is reduced in near future as electricity is a major contributor in life cycle impacts of biodiesel. The impacts from the biodiesel are majorly from oil extraction and transesterification phase in the life cycle, which are due to the use of natural gas for steam and electricity use in these stages of life cycle.