As reflected by this sequence, upon rehydration, the carboxylate and amide PS residues have a greater relative contribution at low PS concentration at the diamond surface. Rehydration of an organo-mineral association enhances hydrophilic interactions by increasing water bridges within the organic layer , ultimately leading to the desorption of hydrophilic components. However, the relatively more hydrophobic methyl ester residues remain at the diamond surface due to stronger hydrophobic interactions through their aliphatic carbons, minimizing exposure to polar water molecules. We can infer that the hydrophilic components within the PS assembly are more prone to water retention when a thinner PS layer is adsorbed on the diamond. The thinner PS layer also has fewer hydrophobic zones to counterbalance the increased molecular disorder from hydration. Conversely, a thicker PS layer stabilizes the organic-organic association with a more extensive network of hydrophobic zones, thus minimizing conformational disruptions due to the variety of inter-and intramolecular van der Waals and hydrophobic interactions. In general, desorption seems to increase the fluidity of organic-organic interactions that promotes conformational changes and self-assembly of PS molecular fragments within the organic layer.