The liquid leakage test is an important evaluation method used to assess the leakage characteristics of the multilayered graft. The test setup consists of a homemade apparatus, including a peristaltic pump, a pressure gauge, and a collection plate to collect any leaked solution. To conduct the test, a 2.5-cm long multilayered graft is prepared and placed in the setup. The graft is then flushed with PBS buffer at a physiological temperature of 37 degrees Celsius. A pressure of 80-120 mmHg, representing typical physiological conditions, is applied to the graft, and the peristaltic pump is set to cycle at a rate of 70 times per minute. The test duration is set to 3 minutes. During the test, any PBS buffer that leaks through the graft is collected using the collection plate. Figure shows the home-made setup for the liquid leakage test. The quantity of leaked solution is measured and expressed as mL/mincm 2 , which represents the leakage rate per unit area. The test is performed on graft samples with and without gelatin coating to compare the effect of the coating. Three samples are tested for each group to provide reliable data. By comparing the leakage rates between the coated and non-coated groups, the impact of the gelatin coating on reducing leakage can be assessed. To evaluate the potential toxic effects of the multilayered tubular scaffold components (extract of the materials) on cells, the indirect cytotoxicity study was carried out according to ISO10993-5 with slight modification, using extract materials and L929 fibroblasts cell line. Briefly, 96-well plate were used to culture 10 × 10 3 of L929 cells in each well using complete growth medium (Dulbecco's Modified Eagle Medium (DMEM), containing 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin). The cells were kept in CO2 incubator over 24 hours at 37 °C. In parallel, tubular scaffolds were prepared with 3 cm 2 of surface area, sterilized using UV light and placed in 24-wel plate. They were incubated in CO2 incubator over 24 hours at 37 °C in 1ml of complete growth medium.