Figure show the XICs of non-hydrolyzed, 1 and 2 hydrolyzed thio-succinimide on THT peptide, respectively. The species profile suggests greater extent of hydrolysis occurred with longer incubation. No obvious non-hydrolyzed product is observable starting from 72 hr incubation. However, the concentration of product containing 1 hydrolysis on THT increased at first, then decreased. The concentration of product containing 2 hydrolysis products increases over time. This suggests both linker-payloads attached to THT peptide are subject to hydrolysis simultaneously. Multiple peaks observed in Figure and 3C were further investigated and discussed in later section. Figure shows the representative mass spectrum of THT + 0, 1 and 2 Hydrolysis products over 0, 6 and 168 hr incubation. The mass spectra confirmed the hydrolysis trends.