Other parameters which could be investigated in this context are different entropic approximations, ligand charge models and the solute or interior dielectric constant . Lastly, another avenue worth exploring from a cost-cutting point of view is to use single-point minimized structures for the MM-GBSA calculations, similar to single-point docking score calculations. There are studies present in the literature which have shown similar correlations between MM-GBSA scores obtained using single-point structures and those using ensembles of MD generated structures. However, recently published literature suggests that most studies still use multiple conformational snapshots from MD for the MM-GBSA calculations .