Using the same method as the control scenario, the Wild type RBD was placed in front of the ACE2-DHF complex, but separated by 6 Ångströms, and then the simulation ran for 100 nsec. The objective was to study the interaction of RBD with ACE2 when DHF acts as a ligand in ACE2. The objective of this scenario is to bound answer the question: Can RBD approach ACE2 to form a stable RBD-ACE2 complex when ACE2 has DHF as a ligand? and if affirmative, study the interactions between RBD and ACE2.