To further investigate the Nuc-DAOTA + for the visualization of different phases of mitotic cell division, the live CHO cells were stained with Nuc-DAOTA + (4 μM) in PBS and incubated at 37 o C for 15 minutes, then washed with Dulbecco´s modified eagle medium (1x). The fluorescence images of Nuc-DAOTA + stained CHO cells clearly identified different mitotic phases, along with a well-identified interphase (Figure ).