The remaining two proteoforms idenNfied differed between SCX and SEC analysis. From SEC-MS analysis the A2G0F_A2G0 glycan pair was observed with consistent levels for the four DP batches and DS, ranging from 4.34% to 6.18%. Through SEC-MS analysis, it was also possible to detect a single A2G0F glycan observed at consistent levels ranging from and 2.08% to 2.63% for the four DP batches and DS. The levels observed for the A2G0F proteoform at the intact level correlates with the levels of unglycoslyated pepNdes observed via pepNde mapping, ~1 %. The levels observed via the pepNde mapping are slightly lower compared to that observed at the intact level, possibly due to overesNmaNng the % using the iMAM approach as only the major glycoforms are observed and reported.