The measurement of dynamic volume by NMR. The segmental dynamics of ligand can be assessed with a different approach based on the motional averaging of 1 H- C dipolar coupling on CH2 units. This is normally achieved by the dipolar chemical shift correlation (DIPSHIFT) NMR experiment which can provide more quantitative measures of the motional amplitude . The DIPSHIFT relies on the C chemical shift to resolve different segments and provides evolution curves resulting from 1 H-13 C dipolar coupling for each segment. The larger curvature of the curve indicates the stronger residual dipolar interaction and the smaller motional amplitude.