To explore enzymatic degradation at mesophilic and thermophilic temperatures, two PET hydrolases, Dura and LCC , were opted for the hydrolysis of PCL and PET (6% crystallinity) films at 37 °C and 65 °C respectively. The hydrolysis activity was quantified by high-performance liquid chromatography with ultraviolet absorption (HPLC-UV) measurements of their degradation products: 6-hydroxyhexanoic acid (HA) and terephthalic acid (TPA; produced in equimolar amounts with the oxidisable monomer ethylene glycol (EG) from PET; Figure and Movie S1).