Analysis by 1 H NMR can produce metabolomic fingerprints for chemometric evaluation and detection of adulteration of C. longa powders, and MS has been utilized to confirm the presence of compounds following structure elucidation by NMR. NMR offers a meaningful advantage due to its simple sample preparation, high reproducibility, and ability to detect diverse plant metabolites simultaneously. Counter-current separation (CCS) has been utilized for fractionation and isolation of 1 and its analogs from turmeric extracts. Notably, CCS can recover essentially 100% of loaded sample, because the stationary phase is liquid and, thus, all retained compounds can be readily recovered during an extrusion step or by washing. The present study employed a combination of CCS for sample preparation and quantitative The results demonstrate that (U)HPLC-UV and 1 H NMR are complementary methods for creating chemical profiles. (U)HPLC-UV detected compounds with high UV absorption at 263 nm as chosen wavelength, but was blind to the presence of fatty and saccharide components. On the other hand, 1 H NMR not only observed the resonances belonging to the compounds detected via (U)HPLC-UV, but provided additional characteristic signals attributed to unknown components that were undetectable and/or unidentifiable by (U)HPLC-UV without the use of identical reference standards.