Since the rHS triplex origami structures assemble at neutral pH and show increased stability under physiological conditions and towards enzymatic degradation, they may have cellular or in vivo applications. Triplex origami structures could, for example, act upon and interact with these environments via the docking of protein-TFO conjugates onto dsDNA scaffolds. Interestingly, many DNA-modifying proteins target the major groove of dsDNA, but this accessibility is blocked in densely triplexed structures, thereby altering native enzymatic activity towards these structures. Transcription can also be regulated by triplex formation and recently it was demonstrated how engineered transcription units are regulated by hybrid RNA-DNA triplex structures formed at the promoter .