The presence of highly charged phosphate groups is the reason for electrostatic repulsion between two strands in dsDNA compensated by the attraction forces described above. All known fractional cohesion parameters are related to different attraction forces, and none define the contribution of repulsive (electrostatic) forces. So, until now, it has been unknown how to directly measure or even estimate the part of electrostatic repulsive forces responsible for maintaining the dsDNA helix. However, the stability of the dsDNA helix at equilibrium results from a balance between electrostatic repulsive forces and the sum of attractive forces defined by measurable or known fractional cohesion parameters described and evaluated earlier in the present article. Based on this balance, we can conclude that in the case of chemical denaturation, the total cohesion parameter for attraction forces is equal to and quantitatively describes electrostatic repulsion forces inside the dsDNA helix. This conclusion is valid for 50% DNA denaturation when, according to expressions 22, 25 and 31, cohesion parameters in solution correspond to similar parameters in DNA.