Finally, the study concludes with the analysis of additional blank, SR and dilution series (SRD) samples. The dilution series is repeated here in a manner similar to best practice for targeted bioanalytical methods whereby calibration curves are analysed at the start and end of an analysis to assess changes in instrument response over the course of the study. The final region of the sequence is reserved for MS/MS data generation from repeat analysis of the pooled undiluted study reference sample. Data dependent analysis (DDA) is routinely used in our laboratory; however, data independent analysis (DIA) methods are equally applicable.