The effect of temperature on urea hydrolysis rates in soil was studied at different temperatures Results showed that the maximum enzymatic activity of urease was observed as with increasing the temperature the enzyme activity also increased towards the hydrolysis of urea. Overall, it is concluded that 37-40 ℃ is the optimum temperature for the maximum enzymatic inhibition of urease. It was also noted that at 50 ℃, the enzymatic activity suddenly decreased for all three districts. As temperature range increase enzyme was deactivated and did not stabilize their 3D conformation. High temperatures for long periods led to a decrease in crop yield . Sha and coworkers demonstrated that NBPT urease inhibitor effectively reduced the ammonia loss of 31.6% at 30 ℃ that was better than lower temperature (20 ℃) . Ding et al. reported that temporal variations of surface soil moisture (WFPS) during maize growing season changed from 75.41 to 20.21% with increasing the temperature ranges from 15 to 30 ℃ for urease and nitrification inhibitor working (NBPT + DCD).