Activation of MEA at constant voltage 0.6 V repeated in exact MEA structure and operation condition but, after a treatment procedure. In first treatment method, electrodes were ultrasonicated in 10% Isopropyl solution for 60 min at 60 o C. In Second treatment method, ultrasonication of electrodes was in water for 60 minutes at 60 o C but, this treatment method has some differences in MEA structure, anode composition is A16: CP (TGP-0120), Pt/C 20%, 30% Nafion, and 1.98 mg DL.cm -2 with 0.396 mg .cm -2 catalyst loading. Also, cathode composition is A16: CP (TGP-0120), Pt/C 20%, 30% Nafion, and 1.98 mg DL.cm -2 with 0.4 DL.cm -2 catalyst loading.