To investigate the performance of all three activation methods and to build specific HR-MS/MS libraries, multiple product ion spectra, with precursor isolation at unit mass, were recorded in positive mode for 168 pesticide standards by LC-MS (Table ). CID and EAD spectra with fragments could be obtained for all 168 pesticides, while for UVPD, 158 spectra showed fragments. All three techniques showed similar and different odd and even electron fragments, and a detailed data analysis is on-going which, will be reported elsewhere. Doubly charged radical cations were observed for 18 analytes and only for EAD activation (Table and Figures ).