The maximum current 𝑖 depends now on the second substrate concentration owing to the presence of the term 𝛽 ≥ 1 in its denominator albeit the whole sequence remains kinetically controlled by the first irreversible step in Scheme 2 as evidenced by comparing Figs. and. Nonetheless, the second step may act by lowering the electrochemical current intensity which is now divided by the factor 𝛽 unless [𝑆 ] ≫ 𝐾 (i.e., when 𝛽 ≈ 1) as evidenced by the above 𝑖 expression in Eq.( ). This indirectly justifies the general use of analyses based on MM characteristics of the first step although PP mechanisms involve a sequence of two enzymatic steps. Yet it is clear that using this MM pseudo equivalence leads to the determination of apparently smaller 𝑉 values.