One of the goals of the Human Proteome Project is to secure confident detections via mass spectrometry of all predicted human proteins and has relied on PeptideAtlas and TPP to provide this compendium since its inception in 2012. In order to ensure the highest quality and minimal false positives, a set of guidelines, known as the HPP Mass Spectrometry Data Interpretation Guidelines 3.0 were developed to guide the publication of new detections as well as the PeptideAtlas and TPP process for reanalysis of datasets.