This might cause lower peak intensities in the higher frequency regions with respect to the lower frequency peaks. In the region from 1400 cm -1 to 1800 cm -1 , a series of peaks appear with two maxima centered around 1460 cm -1 and 1580 cm -1 (figure ). Bands in this region are associated with nucleobase vibrations, such as ring deformations and other stretching ), but cannot unequivocally be assigned