Glyphosate is used to control many weeds. Due to its extensive use in agriculture, horticulture, forestry, and urban environments, glyphosate is frequently detected in soils, water bodies, food, and human fluids. Additionally, transformation of laundry additives in wastewater (e.g., aminopolyphosphonates used as antiscalants) have been identified as a new source of glyphosate in European rivers. Detection of glyphosate and its byproducts, specifically aminomethylphosphonic acid (AMPA), occurs despite their immobilization by microorganisms, abiotic degradation, or retention on metal oxides in soils. Increased public concern regarding its potential toxicity and presence in the environment underscores the importance of research into its persistence and interactions within ecosystems.