The wild-type SaPI-PLC was tested with both POPG and POPC membranes as positive and negative controls, respectively, as this protein binds to POPG and has no affinity for POPC membrane . 1 μs-long simulations reproduce the expected behavior: Stable binding was retrieved in the presence of POPG lipids, while in the presence of POPC lipids bound states are short-lived, and binding/unbinding events are frequent (Figure ). . However, with the less negatively charged bilayer, which includes only 5% PIP2 lipids, the protein does not form a stable complex with the membrane even for 1 μs timescale (Figures and).