We note that the residues between the simulated data and the fit show some systematic deviations, especially around the peak locations. This deviation is attributed to the fact that we have normalized over different maximum distances of the droplets in the field of view, which affects the characteristics of the peak position in the pattern and lead to a shift of the local maxima in the averaged droplet pattern. As the diameter size of the droplets is varied, the number of proteins, localizations per droplet, and maximum distance are affected, resulting in slightly different RPD broadening and peak locations. We neither observe systematic deviations in the residuals of single droplets and fits nor in a field of view with an identical radius of droplets (Supplementary Figure , Figure ). Therefore, we consider the averaging effects as not being critical for the conclusions drawn in our study.