Cubosome nanoparticles are of interest for nanomedicine drug delivery systems. Their high internal surface area gives a high potential drug loading capacity, but the higher curved nature of cubic phase lipid assemblies is also thought to be relevant in inducing topological changes in the cell that facilitate cell entry and endosomal escape. Fundamental studies of LCNP-GUV interactions may assist with understanding different mechanisms of nanoparticle delivery in cells and also unwanted side effects of cell toxicity that needs to be minimised to facilitate translation to clinical applications