Nevertheless, the upper and lower stems neighboring the internal loop were helical, as shown by a complete NOESY-walk (Figure and Table ), interrupted only by the 2×2 GG/GG internal loop. As expected, initial results display that multiple conformational states are preferred by the 2×2 GG/GG internal loop in this NMR construct, which were observed at multiple NOESY mixing times (Figure ). In order to discover the specifics of the preferred states of 2×2 GG/GG internal loop in this construct, we performed site-specific m 1 G mutation studies (infra vide).