In this paper, we used similarly sized liposomes but containing different monoamines, dopamine (DA) and serotonin (5-HT) to simulate vesicular release. The use of liposomes avoids the influence of the vesicular dense core. We observed statistically different dynamics of release for the different monoamines. A finite element method, combined with Monte Carlo optimization, was adopted to estimate the dynamic parameters for release of monoamines from the liposomes during VIEC. We provide evidence that these amines differentially adsorb to the membrane lipids in the liposomes or vesicles changing the dynamics by which they exit the vesicle. By fitting to a desorption model, we calculated the desorption rate constants of these two monoamines from the vesicular membrane lipids. DA has a lower desorption rate constant from the liposome membrane, which leads to slower DA release than that observed for 5-HT, while there is little difference in pore size. Although this work describes the desorption-release process for transmitters exiting vesicles during VIEC, the fundamental aspects can be applied to many aspects of chemical or biological vesicle transport. The results experimentally indicate that the process of vesicular release is complicated by interaction between the chemical cargo and the vesicular membrane, thereby altering exocytotic release dynamics.