Full instrument and method validation have been previously detailed . Linearity has been established by triplicate injections of a 17-point calibration curve, ranging in concentrations from 0.01-1000 ng mL -1 . Inter-and intra-day accuracy and precision was performed via triplicate injections of varying concentration within a 24-hour period and across three consecutive days. The instrument limit of detection (IDL) was determined at the concentration that gave a signal to noise (S/N) ≥ 3, with the instrument limit of quantification (IQL) determined at the concentration that gave a S/N ≥ 10 (table ).