Effect of the noise, batch size, strategy and the acquisition function Lastly, the effect of multiple BO parameters were tested on the BO performance using the following procedure: The homolog scanning strategy was applied to a randomly generated lead peptide with a predicted IC50 ranging from 1 to 20 µM. During BO, modifications were made to one of the following parameters: noise level, batch size, batch strategy, or the acquisition function, while the rest of the BO parameters remained constant (default parameters). During BO, peptide lengths remained fixed.