A mixture chemical solution was prepared using those biological, agricultural, and food chemical powders, each with an initial concentration of at least 1mgmL -1 . A control mixture solution, with or without histamine, was utilized to demonstrate its capability of histamine detection in a mixed chemical solution. These solutions were added to the column, which already contained OA nanozyme/NaAc Buffer/H2O2, and the absorbance peak at 417nm was measured 3 minutes after the addition similar to previous steps. Each measurement was replicated three times (N=3).