Separation of AAV VPs is crucial for full-length VP characterization. However, for certain serotypes such as AAV2, complete separation of VP2 and VP1 proteins can be difficult to achieve on LC-MS platforms. found success in AAV VP separation utilizing DFA as a mobile phase additive for RP chromatography, though without achieving separation of AAV2 VP1 and VP2 proteins . Given that DFA has a similar effect on separation as TFA but without its negative ion suppressing effects during MS analysis , pairing it with HILIC has the potential to provide separation of AAV2 VP2 and VP1 proteins using MS compatible mobile phases.