LNP dispersions were diluted with H2O to 0.01 % w/v before one droplet of approximately 10 µL was applied on a clean mica surface and dried overnight under ambient conditions. The AFM measurements were carried out using a MultiMode 8 atomic force microscope equipped with a NanoScope V controller (Bruker Corporation, MA, USA) in tapping mode in ambient air using HQ:NSC15/Al BS tapping mode probes (MikroMasch, CA, USA). Image processing and calculation of radii based on width of the particles was done in Image J.