It is becoming increasingly clear that modeling of realistic membrane environments is important in the study of transmembrane proteins . Previously, Ingólfsson et al. have used the Martini 2 force field to build a standardized and simplified mammalian plasma membrane model that contains eight different types of lipids asymmetrically distributed across the leaflets . Here, we adapt this plasma membrane model (Figure ,A) to the Martini 3 force field by following a protocol recently presented by Ozturk and coworkers . This typical mammalian plasma membrane model has an extracellular leaflet enriched in SM lipids with saturated tails (di-C18:0) and cholesterol, whereas its cytoplasmic leaflet mostly contains unsaturated lipids, some of which are composed of negatively charged headgroups, such as PAPS and SAP6. The number of unsaturated bonds per tail is 1.8 times higher in the cytoplasmic leaflet than in the extracellular leaflet, consistent with a recent experimental study on the plasma membranes of human erythrocytes .