NOE studies. Upon obtaining the three macrocycles, their difference in preorganization and conformational flexibility suggested by modelling was investigated in DMSO-d6 through 1 H NMR 1D-ROESY experiments (Figure ). When inverting the signal of the amide NH proton (Hc) of macrocycle 1, a Nuclear Overhauser Effect (NOE) was only observed with phenanthrene proton Hd, consistent with a conformation where all the amide NHs (Hc) are pointing towards the center of the cavity (Figure ).