The thermo-and pH-responsive characteristics of PNA, P-TN, P-TN-Dox, and P-TN-Dox@CM nanogels were evaluated. As presented in Figure , the hydrodynamic diameter of PNA nanogels decreased with the elevation of temperature, indicating a temperature-dependent behavior. Such a phenomenon was assigned to the impaired hydrogen bonds between hydrophilic groups of PNA nanogels and water molecules at elevated temperature, which induced a domination of hydrophobic interactions in the hydrophobic groups of PNA nanogels, thereupon then leading to a phase separation and shrinkage of PNA nanogels. Meanwhile, the hydrodynamic diameter of PNA nanogels increased when the surrounding PBS buffer pH value elevated from 5.0 to 7.4. The ionization degree of carboxyl groups of PNA nanogels in pH 7.4 was higher than that in pH 5.0, resulting in the improvement of electrostatic repulsive force within the nanogel networks. Accordingly, the hydration of nanogels enhanced and gave rise to the swelling and stability of PNA nanogels. In addition, the hydrodynamic diameters of P-TN, P-TN-Dox, and P-TN-Dox@CM nanogels in different pH and at different temperatures exhibited the similar trend as the PNA nanogels (Figure ), displaying that the encapsulation of photothermic agent of TN and chemotherapeutic drug of Dox as well as tumor cell membrane cloaking did not affect the thermo-and pH-responsiveness of composite nanogels.