Water-in-oil emulsions are inherently unstable and tend to separate into water and oil over time. Amphiphiles stabilize these emulsions by forming micelles around water droplets, increasing surface heterogeneity, and breaking the droplets into smaller clusters. Although the effects of amphiphiles on emulsions are well understood, their microscopic mechanisms remain unclear. Emulsions are crucial in various fields, including chemical separations, oil recovery, pharmaceuticals, and food chemistry. Understanding the structure and dynamics of water in the confinements of the nanodroplets is necessary to improve emulsion properties and controlled separations. At elevated concentrations, amphiphiles adsorb at the nanodroplet surface and lead to the fragmentation of droplets into smaller clusters. This modulation of droplet behavior is due to amphiphile effects on surface heterogeneity and the reduction of surface tension. For example, Smit et al. performed simulations to find oscillating water density on the adsorption of micelle on the water/oil interface causing a spontaneous formation of surfactant monolayer, which was related to an unexpected increase in the neutron reflectivity above critical micelle concentration. However, the perturbations in the interfacial water structure and the formation of water protrusions have not been explored. Recently, it has been shown that increasing the concentration of amphiphiles at the water/air interface leads to enhanced surface fluctuations and increased surface curvature. The presence of air, rather than oil, does not facilitate water transport and suppresses the formation of protrusiontype structures, which are considered in this study. It is also demonstrated that the surfactants at the water/oil interface enhance heterogeneity within the interfacial water structure and drive water transport into organic solvents through the surfactant-water dimer. A similar mechanism was observed for the transport of ion pairs into the oil phase. In this study, we aim to understand whether a similar mechanism might be relevant for the amphiphile driven transport of water from the water nanodroplet, leading to the formation of daughter droplets. Enhanced surface heterogeneity enables amphiphiles to break nanodroplets into tiny water clusters of various shapes and sizes. Importantly, the water droplets in water-in-oil emulsions range from nanometers to micrometers. For larger droplets, amphiphile self-assembly and droplet disintegration can take tens of seconds, while for nanometer-sized droplets, these processes occur much faster and can be simulated computationally. Additionally, enhancing thermal fluctuations through heating can further accelerate the amphiphile-assisted droplet disintegration process. Therefore, in this work, we explored the microscopic mechanisms of amphiphileinduced disintegration of water-in-oil nanodroplet under different temperature conditions, with a primary focus on amphiphile self-assembly, its effects on nanodroplet curvature and surface area, surface heterogeneity, and the transport mechanism of water between the nanodroplet and oil phase.