First, the required amounts of polymer, DTT, LAP and phosphate-buffered saline (PBS) to obtain a 4% w/v HA-X solution were calculated using the functionality Fene and the resulting MW per alkene unit of the specific batch to ensure that the final thiol:ene ratio is 1.00. HA-AE, HA-MA or HA-NB were dissolved in PBS, pH = 7.3 on a shaker at 350 rpm for 60 min, excluded from light.