The RNA concentration comparator had non-zero offset (𝜎 ≠ 0) (Figure ), and the simulation results suggested that 𝜎 changed depending on hybridization rates or strand displacement rates of linker DNAs (Figure and Supplemental Figures ). Because the hybridization and strand displacement rates of DNAs depend on their sequence and length , these results suggest that the non-zero offset was probably due to the sequences of the linker DNA nanostructure. Previously, Saleh et al. and Sato et al. have shown that differences in the sequences of DNA nanostructures changed the kinetic and thermodynamic properties of DNA droplets. To further control the DNA droplet dynamics, the influence of DNA sequences on the kinetic properties of DNA nanostructures must be clarified.