We compared the results of canSARchem and OpencanSARchem pipelines for ChEMBL 32 for three relevant reprsentations: 1) SC, 2) UCR, and 3) AR. The comparison is based on three key criteria: 'Total Difference (%)' which reflects the percentage of cases where the Tanimoto similarity between SMILES from the two pipeline is less than not be the same in another, leading to different tautomeric equilibria and Gibbs free energies , . Our modeling utilized an implicit water solvent a reasonable representation of biologically relevant conditions. The mean difference (dashed line) for centroids is 1.4 kcal/mol and for rigids is 2.4 kcal/mol. The median is shown as a solid line. To determine if the observed mean or median difference in Gibbs free energy is statistically significant, we applied appropriate statistical tests.