As outlined above, cSeNPs can react with transition metals such as Zn, Cd, Ag, and Cu to form highly fluorescent metal selenide nanoparticles (e.g., Quantum Dots). We assessed the cSeNPs converted to fluorescent metal selenides in live cells, followed by their imaging in both fluorescence and electron microscopy. E. coli expressing FtsZ-cSeNP were grown with 10 mM SeO3 2-supplementation for 1 hour. The cells were collected by centrifugation, washed and resuspended with Luria Broth (LB), and allowed to recover for 20 minutes. Zinc Acetate was added to the liquid culture to a concentration of 1mM, and cells were grown for an additional 2 hours. Cells were then collected by centrifugation, fixed with glutaraldehyde, and imaged. The higher magnification electron microscope image in panel F shows E. coli cells with higher contrast densities at the midpoint of 3 dividing cells and at the poles of 3 other cells. These densities correspond to known distributions of FtsZ, and we attribute these densities to SeNPs co-localized to FtsZ. The fluorescence images of panel E show a relatively uniform fluorescence background across the entire cells, with bright emission at the midpoints of 2 of the dividing cells and at the pole of one of the other cells. The overlay image of panel F confirms that all the high florescence regions correspond uniformly to the locations of SeNP density observed in electron microscopy. The control electron image shows cells with a few regions of slightly higher contrast, which we attribute to an excess of protein or inclusion bodies in those locations as an artifact of overexpression.