However, these AMS screens were targeted towards likely active compounds. Therefore, we applied a more stringent cutoff of 50%, which is the smaller % inhibition distribution mean from the two replicates (Figure ). In addition to requiring at least 50% inhibition in both replicates, we also required active compounds to not match a PAINS filter. 4.9 Enamine REAL Computational Scalability, Compound Selection, and Screening