In order to test this hypothesis, we modelled these experimental conditions in our digital simulations by using 585 and 58500 laser pulses to take into account the experimental exposure times (50 ms and 5 s, respectively) and laser repetition rate (11.7 kHz). Fig. shows the results of these simulations for the 100× NA 1.40 objective PSF with write spot spacings of 0.6 µm (left) and 1.8 µm (right), the approximation in the choice of the simulated distances being imposed by the characteristics of our sampling grid (see experimental section).