The results of the automatic library generation applied to all seven compound libraries are summarized in Extended Data Table and . This demonstrates the great potential of our newly developed method to increase the coverage of the chemical space within spectral databases. The chemical space coverage is illustrated in Extended Data Fig. as a TMAP projection. This projection includes all chemical structures from MS n Lib, the three open spectral libraries combined, mzCloud, and NIST23, representing the entire chemical space covered by spectral libraries. Each spectral library encompasses a distinct area, with NIST23 demonstrating the most extensive coverage due to its high number of unique compounds. The chemical space TMAP projections reflect the uniqueness of each spectral library and highlight in grey branches how complementary their coverage is. By integrating the newly created MS n Lib with the three largest open spectral libraries, we achieve a comparable chemical space coverage. To facilitate public access to the library, we uploaded the data to open libraries, including GNPS, and Zenodo (see Data Availability section).