JigSAP capable of the efficient release of full-length proteins can be used for neuronal regeneration (Fig. ). When phosphate-buffered saline (PBS) (Fig. and), 1.0 wt% JigSAP (Fig. and), VEGF-bound JigSAP (Fig. and), 1.0 wt% JigSAP and 1.6 ng of non-tagged VEGF (VEGF + JigSAP; Fig. and), or 1.0 wt% JigSAP incorporating 1.6 ng of VEGF-bound JigSAP (VEGF-JigSAP + JigSAP; Fig. and) are injected into the injured cortex of a mouse model of ischaemic stroke produced by distal middle cerebral artery occlusion, the number of double-positive cells with laminin, an endothelial cell marker, and a thymidine analogue (EdU), increases in the injured area when VEGF-JigSAP and JigSAP peptides are used (Fig. and). These results imply the promotion of angiogenesis in the injured area by incorporation and release of VEGF. The incorporation and releasing property of JigSAP demonstrates wide applicability to controlled sustained release of various full-length growth factors, which should be useful as artificial scaffolds for tissue regeneration.