The efficiency of fluorescence quenching is influenced by the concentration of the NS@DA and the incubation temperature because the concentration and temperature affect the adsorption of the FAM-ssDNA probe on the NS@DA. Fig. shows that the QE increased with increasing nanoparticle content. When 10 µL of DFNS@DA was used, the QE reached to more than 90%. For the PDA-coated nonporous nanosilica, the QE was only 85% with 16 µL of SNS@DA. This result confirms that the PDA-coated DFNS is a more effective quencher than the coated nonporous silica. In Fig. , the QE of DFNS@DA was measured at three different temperatures. The QE at 18 °C and 30 °C were almost the same and were slightly higher than at 4 °C. For SNS@DA, the QE increased with the incubation temperature, suggesting that on the nonporous particles the ssDNA adsorption is more entropy driven. types of DFNS@DA, DFNS-20@DA exhibited higher recovery efficiency (RE) than DFNS-4@DA, although both had similar quenching kinetics. In contrast to the PDA-coated mesoporous nanoparticles, the PDA-coated nonporous particle SNS@DA exhibited poorer fluorescence recovery. cDNA concentration changed from 1 to 1000 nM. For SNS@DA-based sensor, the fluorescence signal started to increase at cDNA concentration of 100 nM (Fig. ). Therefore, the DFNS@DA-based sensors have a wider detection range.