In summary, we performed CF-SMD simulations for the NaCl system and analyzed the dependency of dissociation time on a constant force using Bell's and DHS models. The analysis allowed us to extract the dissociation time, location of the activation barrier, and activation barrier height. To scrutinize the ability of the CF-SMD simulation to further calculate the dissociation time, subsequent sections deal with the protein-ligand systems. a Error factor was defined by 𝑘 (--/𝑘 (-- , where 𝑘 (-- is the dissociation time of MD1(eq) or Md2(eq).