The number of PSNPs, 1000, was sufficient to observe the stationary distribution of thermophoretic depletion after a sufficient time had elapsed since the start of thermophoresis. By t = 30 s, as shown in Fig. , the contribution of thermophoresis becomes pronounced, with PSNPs rapidly moving away from the laser spot. At t = 60 s, as shown in Fig. , while the changes become more gradual compared to the earlier stage, it is evident that the region depleted of PSNPs due to thermophoresis has expanded compared to the t = 30 s condition. On the other hand, the range of PSNP depletion observed in Figs. 2d to -e remains almost unchanged from that in Fig. .