In the first 10 µs of the MD simulation completed by the OL3 force field, no major structural distortions were observed, and the helicity of the RNA construct was conserved (Figure ). adopt anti-syn, syn-anti, and anti-anti orientations, respectively, at 55%, 16%, and 26% (Figure and Table ). In contrast, the configurational preferences of 2×2 GG/GG internal loops have not been studied greatly as evidenced by the lack of structures available in the literature.