On for PKB there is an electronegative binding pocket formed between the Glu-A-127, Glu-A170, and Asn-A-171 that is be categorized as key Anchor-category residues in the ), stated that upon targeting the electronegative binding pocket (between the Glu-A-127, Glu-A170, and Asn-A-171) was found to gain 30-fold affinity and selectivity for lead compound. We include the analysis of the holo-complexes using FMOPhore on the holo-complex structures suggest that the lead compounds 2UW4, 2UW5, 2UW6, 2UW7, and 2UW9, kept the starting scaffold of the primary hit fragment (2UW3), growing the scaffold to reach to this critical binding site residues that was reflected with a better binding interaction energy, (Figure . A and B) and (Supplementary files 5.).