To promote a potential synergic effect based on ECM digestion enhancing drug penetration and on the other hand, pH-dependent drug release, we physically bonded two kinds of cargo-loaded Psomes to the surface of microcarriers in a heterogeneous manner pursuing a surface with possible dual therapeutic effect (CU(βCD)2-Psomes/HYAL-Psomes or CU(βCD)2-Psomes/MMPsomes, respectively). We used SEM imaging to determine if the Psomes with dual cargo loads were effectively immobilized on the surface of the microcarriers (Figure ). SEM images demonstrate an effective homogeneous coating of dual cargo-loaded Psomes on the surface of the microcarriers. To further investigate the cargo-loaded Psomes and the distribution of the various nanocontainers immobilized, an additional characterization technique, confocal scanning laser microscopy (CLSM) was utilized (Figure ). For this purpose, both Psomes were prepared labeled with different dyes that allow us to see the presence of both potential nanoreservoirs. The preparation was carried out in the same manner as for SEM studies, but in this case using Empty-Psomes, FITC-Empty-Psomes A and RhB-Empty-Psomes D.