10 mg/mL solutions were prepared as described in Section 1.3, using either NaOH or TBAOH. Gel noodles were formed using the injection of 10 μL of 2NapFF-Na or 2NapFF-TBA solution into trigger medium (50 mM CaCl2 adjusted to pH 10.5 with the corresponding hydroxide (NaOH or TBAOH)). A 2-20 μL pipette was used to perform a static injection as has been detailed in previously published work. For heat/cool noodle experiments, noodles were prepared using the syringe-pump and spin-coater set-up as previously described. The flow rate was set to 100 mL/hr with the spin-coater rotating at 100 rpm. The needle connected to the syringe pump was held vertically into the spinning CaCl2 solution (0.5 M). For the heat/cool noodles. the CaCl2 concentration was optimised to allow formation of mechanically robust noodles. When 50 mM CaCl2 was used, gelation was too slow and continuous noodles did not form. Noodles were formed from solutions of 2NapFF (10 mg/mL, pH 10.5) prepared using either NaOH or TBAOH as the base. The base used determines the counterion present within the solutions. The 2NapFF-Na noodles were strong enough to be lifted out of the petri dish while the 2NapFF-TBA noodles broke down when we attempted to remove them from the petri dish. We therefore prepared two sets of noodles for each counterion, a control sample and a heat/cool sample. The gel noodles were prepared for 3 second for each sample. This gave noodles of a reasonable size to allow imaging along the entire length of the noodle in each condition. For each sample, the noodles were first imaged under a light microscope. Then the Petri dish in which the noodles had been prepared was transferred directly to a water bath heated at 60 °C (or kept at room temperature for the control samples) and left for 1 hour. After heating, the samples were left to cool for 1 hour before being imaged again under the microscope to obtain the after images.