From the 315 proteins that had simultaneously less than 20 variants and less than 10,000 bioactivity data, only 100 displayed a variant bioactivity percentage equal to or greater than 10% (Figure ), and only 10 of these had more than 1,000 bioactivity data points. For reference, we consider 1,000 data points as an arbitrary threshold to enable bioactivity modelling. Save for the exceptions mentioned above, generally higher variant bioactivity percentages correlated with lower total bioactivity data, regardless of the number of variants annotated (Supplementary Figure ). From the total of 335 proteins in the dataset, only 32 showed as much or more bioactivity data for variants than for WT (i.e. 50% variant bioactivity percentage or higher), and out of these, only three had more than 1,000 bioactivity data points, namely IDHC (seven variants apart from WT), BRAF (one variant), and RPKS6B1 (two variants), and variant bioactivity percentages of 86.29%, 60.27%, and 55.21%. Labelled are proteins with a variant bioactivity percentage higher than 10% and more than 500 data.