In the experiment, 100 g of toasted Ilex paraguariensis leaves obtained in a local market was infused in 150 ml freshly boiled distilled water and 150 ml of ethanol for 20 min. The infusion was filtered through quantitative filter paper, and the volume was reduced to 50 ml under low pressure. After reducing the volume, the solution was subjected to freeze drying and lyophilized to obtain a dry extract.