As demonstrated in our study, there is a concrete need for such a purification method that is more effective than conventional centrifugation. While centrifugation is suitable to roughly separate monomeric AuNP and C/S particles, a non-negligible amount of AuNP remains in the We demonstrate that the SERS intensity increases by 25% with the purity of C/S particles, which motivates the application of AFFF for this separation task.