There are two distinct ways to obtain an MFEP. The first is to guess an initial string of coordinates, and then the structures that correspond to each of the points on the string are generated to perform MD runs, where the trajectories can provide useful information on how to subsequently modify the string. Examples that fall into this category include the fly string method, Finite Temperature String (FTS), swarm of trajectories and so on. The second way is to first obtain a converged FEL given that the simulation time is long enough so most of the configurations are sampled, and then the free energy F(q) that corresponds to each of the combinations of the CVs q can be expressed as