This means that the properties in the DNA are equal to the properties of the solution at the melting temperature, allowing us to uncover the intermolecular forces what is in the DNA using chemical denaturation. When we use the solubility parameters, they are only applicable to small, uncharged molecules, but by knowing that at the equilibrium point of 50% denaturation, the properties of the solution are identical to the properties of DNA, we can apply these measurable solubility parameters in the solution to deduce forces inside DNA.