Three of the anomeric protons (from D-Manp moieties A-C) show correlations in the C-HMBC spectrum to the p-nitrophenyl group, identifying substituted D-Manp residues. Information on the connectivity between the different D-Manp residues were then obtained from elevated C chemical shift of the substituted carbon , direct observation of heteronuclear three-bond correlations over the glycosidic linkage, and observation of NOE between protons close in space. The pNP-linked D-Manp units A and C, together with the remaining five D-Manp units (labelled D-G & I) could be shown to belong to D-Manp units of four reaction products, i.e. two dimannosides and two trimannosides,