Hence, due to their high stability and intrinsic enzyme-like properties, the nanozymes were used for different applications, especially for constructing sensing assays for a wide variety of analytes, e.g., amino acids, glutathione (GSH), tetracycline, metal cations, glucose, H2O2, explosives, malathion , and new SARS-CoV-2 as after the first report of COVID-19 . However, the researches focusing on the inhibitory effect of inhibitors on nanozymes activity are limited to a few reports. Hence, in this continuation, the inhibitory effect of captopril molecules on the nanozymatic activity of peroxidase-like nitrogen-doped carbon dots was studied. In this regard, the carbon dots were synthesized via a simple solvent-free method and then characterized for their FL properties and enzyme-like activity. Thereafter, the effect of captopril molecules on their nanozymatic activity was evaluated by calculating their nanozymatic activity in the presence and the absence of captopril. Besides, the nanozymatic reaction rate was also determined by quantification of produced oxidation product of TMB in the presence and the absence of captopril to explore more precise on the inhibitory effect of captopril molecules on the activity of peroxidase-like nitrogen-doped carbon dots.