The significant protonation of the polymer's tertiary residues was responsible for the observed abrupt enhancement of the DT-loaded micelles' zeta potential toward positive values (-7.4 mV and -5.4 mV for Brij 58 and Brij S20, respectively) when the pH was lowered . Because the zeta potential values are below the threshold (around -20 mV) for being colloidal, the micelle assembly was compromised . On the other hand, increasing the system temperature causes the micelle to enlarge as a result of water penetration and leaking of the entrapped chemical . As a result, when temperature rises, the amount of DT that is contained inside the micelle significantly drops.