tide/MCL-1 Complexes. AlphaFold2 (AF2) has emerged as a powerful tool to predict threedimensional structures of proteins and protein/petide complexes. AF2 was used to generate models of the individual coiled-coil scaffolds and their complexes with MCL-1 (Fig. ). For each scaffold and scaffold/protein complex the average of relative predicted aligned error (pAE) and average per-residue model confidence score (pLDDT) were used to evaluate the confidence of assembly formation. Models were first generated with the peptide scaffold sequences alone, varying the total number of copies of peptide from one to four as appropriate to assess the most stable oligomerization state of each sequence.