Water, acetonitrile and isopropanol (Optima LC/MS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Ammonium acetate (LiChropur) and DMSO were provided by Merck (Darmstadt, Germany). LC/MS-grade acetic acid was obtained from Biosolve (Dieuze, France). The mix of 69 deuterated lipid standards was purchased from Avanti Polar Lipids (UltimateSPLASH ONE, Alabaster, AL, USA). Mass spectrometer calibration solution was supplied by Thermo Fisher Scientific.