In conclusion, we presented a new DNA-protein nanogel platform for the transport of active loaded proteins into living cells. The loaded proteins have been efficiently internalized by the cells while keeping their specific properties, which underlines potential therapeutic interest of lipoproplexes for delivery and co-delivery into the cells of active enzymes. We anticipate that DNA-protein nanogels could be employed as nanoscaffold for the transport of other biological and chemical entities, not only by incorporating them into the nanogel by streptavidin-biotin binding, but also by orthogonal binding into DNA double helix (i.e. intercalative drugs, such as doxorubicin). Moreover, the commercial availability of biotinylated, streptavidinated or strep-tagged recombinant proteins, nanoparticles, aptameric sequences or antibodies for the specific recognition of target molecules, opens up the possibility of using nanogels as universal scaffolds with a high degree of functionalizability that not only could encapsulate a number of desired features in a single nanosized material, but also could protect the incorporated entities from biological microenvironment. All these results underline great potential of using DNA transfection agents for soft DNA nanomaterials delivery.