These considerations agree with our experimental findings. The melting temperature of DNA does not change in the presence of COSAN, showing that COSAN has no significant effect on the stability of DNA strands. Furthermore, ITC, equilibrium dialysis and NMR measurements showed no binding between COSAN and DNA. In equilibrium dialysis, only at a high [ctDNA(pb)]/[COSAN] ratio of 20 did a small fraction of COSAN bind to DNA, which might have even been an artifact of interactions between COSAN and residual proteins present in the DNA sample. And intercalation or minor groove binding would have placed COSAN in the chiral environment of DNA and align this molecule with DNA in the Couette flow, which we did not observe in CD and LD measurements, respectively.