However, even with peptide-level splitting, some additional information can potentially leak through if the training and test sets both contain spectra generated from the same experiment. In principle, a machine learning algorithm may be capable of identifying distinct properties of spectra from a given run, for example, related to calibration of the m/z axis. If the model can effectively say "This spectrum resembles these other spectra that I saw during training," then it can again achieve an unfair advantage, because in practice true "test" spectra will never come from the experiments used during training. Hence, to avoid batch effects and peptide-level leakage, a proper train/test setup should ensure that the training set and test set do not overlap in either sense. The revised nine-species benchmark dataset addresses this problem by splitting at the species level and eliminating overlaps at the peptide level .