Table Finally, we scaled-up the model reaction on butanol (1a), and we conducted the process at 20 mL scale. To reduce the carbon cost of the reaction, we decided to use only 1.1 AMFC equivalent, compared to the 5 equivalents used in small-scale reactions (Figure ). We observed a slightly lower butanol conversion, with 86 % yield in butanoic acid in 24h. Not surprisingly with just 1.1 equivalent AMFC in the reaction media, only 36 % amide formation was achieved after 24 h in the 2 nd step (Table ). While this result may seem modest, it is very promising. There are several possibilities for improvement, while maintaining a low carbon cost, such as implementing an ATP regeneration system, as recently highlighted by the authors of this article.