We aimed to calculate the reference BHs and REs for this set using DLPNO-CCSD(T) with the TightPNO thresholds, which is regularly used in benchmarking as a cost effective alternative to "gold standard" CCSD(T) results. In our previous benchmark study on enzymatically catalysed reactions, we did an analysis of various extrapolation and estimation schemes to determine a protocol for calculating reference values.