• dUTpase playing an essential roles in cellular nucleotide metabolic process, it catalyze the electrolyze of dUTP to dUMP and pyrophosphate (PPi). • Firstly, the enzyme supplies dUMP to synthesize dTTP ,a DNA building block, and secondly, the enzyme lowers the dUTP/dTTP ratio in the cell, so preventing the misincorporation into genome DNA . • And deficiency of the enzyme would result into too high uracil content in DNA,The thymine-less cell death due to overdosed DNA repair activity • Because of their essential role, it has been suggested that as potential drug targets for the therapy of cancer, and infectious diseases.