Critical quality attributes (CQAs) in the bioprocessing industry refer to specific parameters that have an impact on the quality, safety, and efficacy of the biotherapeutics products. These attributes are required to be closely monitored and controlled to ensure the integrity of the manufacturing process and that regulatory requirements are met . These CQAs are typically monitored in quality control (QC) laboratories using analytical assays that are designed to specifically evaluate and monitor these attributes. The analytical assays must be executed in a current Good Manufacturing Practice (cGMP) environment and the methods must be robust, reproducible, and fit for purpose. The challenge, however, of evaluating CQAs of biotherapeutics is due to the inherent complexity of these products.