For 4549 macrocyclic peptides analyzed during physiochemical screening, reported in a previous study , received 10 mM stock solutions were diluted with MeOH:ACN:DMSO (80:10:10) to yield 100 µM solutions. This dilution was conducted using a Tecan Freedom EVO® Liquid Handler or with a Rainin E4 TM XLS Single Channel 200 µL Electronic Pipet. Crude reaction mixtures of the 104 macrocyclic peptides from purification studies were analyzed using the 8 min method without any additional preparation.