To provide an assessment of the dynamics of the peptides within the NP, we consider the time-evolution of each peptide's local environment (defining the instantaneous local environment as the polymer beads found within a cutoff distance of the peptide at a given time). We calculate the autocorrelation function (ACF) of the local environment (see Figure ). Both the peptides in the core and those at the core-corona interface readily exchange between different polymer beads, indicating that they are not static within the NP; the local environment of the two peptides in the core of the NP changes more slowly than those peptides at the core-corona interface, showing they are less dynamic, as might be expected. To investigate the specific conformations that the polymers adopt within the NP, we applied a two-step unsupervised machine learning protocol consisting of dimensionality reduction with UMAP and clustering in the subsequent embedding using HDBSCAN (for the full methodology see the SI section 'Dimensionality reduction and clustering'). Figure (a) shows the four clusters that were identified via this procedure (0.41% of molecular conformations were not clustered). Each cluster groups together polymers of similar conformation. The polymer conformation is defined by the end-to-end distances of both the PLGA and PEG blocks. Cluster 1, the most frequently identified conformation, has a collapsed PEG block and an extended PLGA block. Cluster 2 possesses an extended PEG block and collapsed PLGA block, while in cluster 3 both blocks are collapsed. Cluster 4, the least frequently observed conformation, has a particularly extended EO block , as well as an extended PLGA block. Each cluster therefore represents different polymer conformations, which can be readily understood in a physical sense. The embedded space and the average block distances of each cluster are shown in Figure . We then calculated the intrinsic density of each conformational cluster (Figure (b)) to reveal the spatial distribution of each conformation within the NP, with respect to the core-corona interface. We note that cluster 1, with its extended PLGA block, exhibits high density in the center of the NP core.