Here we tested MAP4C with the reference benchmarking dataset of Riniker and Landrum for small molecule drugs, which considers 118 active and decoy datasets taken from DUD, MUV, and ChEMBL. For larger molecules, we used our previously reported set of 60 different randomly chosen 10-, 15-and 20-mer peptides mixed with either random single point mutants (30 sets), or sequence scrambled analog (30 sets), for which we challenge the fingerprint to retrieve BLAST search analogs. Both of these benchmarks tested the ability of the fingerprints to retrieve bioactive analogs without consideration of stereochemistry. Here we compared the performance of MAP2C, MAP4C, and MAP6C with their respective achiral counterparts, as well as with reference binary fingerprints ECFP4, ECFP6, and AP, and their corresponding chiral versions (ECFP4C, ECFP6C, and APC).