Since the polymer is known to form a nanometric film on the surface of silica, we have utilized gold nanoparticles (GNPs) encapsulated within a silica shell through a sol-gel process. The silica shell provides stability, biocompatibility, and a versatile platform for further functionalization with copolymer of dimethylacrylamide. In this work, we demonstrate that dimethylacrylamide polymers can also be used to coat AuNP lacking a silica shell. Uncoated gold nanoparticles present a unique opportunity for modification with thiol-bearing reagents. However, the inherent challenge lies in the limited colloidal stability of AuNPs during the conjugation process, which may lead to aggregation. Aggregation propensity was observed also during the polymer coating stage. Indeed, the polymers employed in this investigation offer a significant advantage, thanks to their exceptional versatility. This adaptability allows for modifications to their composition, either during synthesis or through post-polymerization processes. To coat AuNPs without inducing aggregation, we introduced an ionizable monomer into the polymer backbone. This strategic modification not only ensures the stable coating of AuNPs but also creates opportunities for diverse functionalization, thereby enhancing their applicability across various fields. The polymer film bearing PEG-azide functionalities allows covalent binding of proteins and DNA modified with dibenzocyclooctyne (DBCO), a group characterized by its high reactivity towards azides via a copper-free strain-promoted alkyne-azide cycloaddition (SPAAC) reaction . This reaction proceeds rapidly and efficiently without the need for a copper catalyst, making it particularly attractive for bioconjugation and labeling applications in biological systems where copper could be cytotoxic.