Secondly, another path of T = 200 segments is repeated where the electrostatic interaction is turned on, in the same way as for the growth of the molecules. The water solvation work is summed during the process of growth and charging in the same way as equation 5. (or -6.334 kcal/mol), which is consistent with previous studies and confirms that the NCMC method is versatile as it can be used to performed both alchemical transformation and particle insertion.