Cryogenic ion vibrational predissociation (CIVP) spectra were collected using a home built photofragmentation mass spectrometer described previously. Ammonium-adipic acid clusters were generated by ESI of a 2.5 mM solution of adipic acid in 20:80 water:acetonitrile with 0.1% formic acid. These ions were then introduced into the vacuum system and accumulated in a room temperature octopole trap. The RF amplitude and bias of this trap can be tuned to either promote or suppress CID. Ions are then routed from this trap to a variable-temperature octopole ion trap capable of achieving temperatures between 3 -310 K. Messenger tagging with N 2 was achieved by storing the clusters of interest in the ion trap held between 20 -50 K under a pulsed helium buffer gas. Here N 2 is likely sourced from background gas in the vacuum chamber (base pressure 10 -8 torr). The tagged ions are extracted from the ion trap and orthogonally accelerated into a tandem time-of-flight mass spectrometer consisting of both a linear and reflectron stage. As the ions of interest cross the time focus of the linear stage, they are intersected by an infrared laser pulse from an Nd:YAG pumped infrared OPO/OPA (LaserVision) laser system. The reflectron stage separates fragments from undetached parent ions, which are then guided into a microchannel plate detector. Spectra are obtained by dividing the integrated intensity of the fragment by the sum of the integrated intensities of both the parent and the fragment. A laser power correction is then applied.