Capture antibodies at 1 mg/mL in 1× PBS, pH 7.4 and 2.5% (w/v) sucrose were striped (ZX1010, BioDot, Irvine, CA, USA) on nitrocellulose CN95 and dried at 25°C for 30 min. The control line was striped at 0.75 mg/mL donkey anti-chicken IgY (Cat. No. 703-005-155, Jackson ImmunoResearch, West Grove, PA, USA). For antibody screening, the nitrocellulose was unblocked. The test and control lines were located at 8 mm and 13 mm from the upstream edge of the nitrocellulose membrane.