A mass analysis experiment was created in BPF to perform identification of full-length AAV viral proteins and their isoforms, using the parameters summarized in Table . Multiconsensus analysis was performed to analyse the triplicate sample injections together. The resultant list of identified protein features was filtered to include only identifications found in all three triplicate samples, with a score ≥ 60. This high score was selected to ensure only the highest quality identifications in runs performed at all column temperatures, as components with scores below 60 were found to have poor deconvoluted spectra or could not be identified based on the PTMs found during peptide mapping.