We chose to construct the device around a vertical flow configuration (Fig and). Although vertical flow assays are less common than their lateral flow counterpart, they have had considerable recent commercial success due to the faster assay time resulting from enlarged flow cross sections . Our design sandwiches a nitrocellulose capture layer between a receiving sample pad and the LIG-E, thus avoiding tedious chemical functionalization on the electrode. The working principle relies on the capture of ssDNA reporters on the antibody-functionalized nitrocellulose pad (Fig. ). Specifically, in the presence of HPV16 amplicons, reporters are cleaved and labels flow through the LIG-E without generating signal. Conversely, for a negative sample, reporters remain uncleaved, thus ensuring ample binding sites for labelling. Labelling and signal generation then take place through amplification of ALP, a standard enzymatic label for linked immunoassays (Fig. ). We designed the device to saturate after the addition of enzymatic substrate and quantified the amount of captured label by square wave voltammetry (Figs. and). For the direct detection of CRISPR reporter, we observe a lower detection limit of 67 pM and upper limit of quantification of 60 nM of reporter (Figs. and). Combined with DETECTR, we observed strong quenching of the voltammetry signal in the presence of HPV16 (Fig. ) and achieved a lower limit of detection of 1 copy μL -1 of HPV16, the WHO reference standard for HPV16 (Fig. and). Remarkably, we found that these results were comparable to benchtop fluorescence data using a plate reader (Fig. ). This assay demonstrates the usefulness of the LIG-E platform to perform capture assays for high-performance molecular detection, without sacrificing the operational simplicity of colorimetric devices.