Triplex origami cannot assemble shapes as arbitrarily as conventional DNA origami, because the inherent stiffness of dsDNA introduces an energy barrier that must be overcome to enable self-assembly. The height of this energy barrier is directly related to the number, spacing, and sharpness of dsDNA scaffold loops. The force that overcomes it is some non-trivial combination of the staple binding affinity, crossover density, and the overall cooperativity involved in the folding. The energetically demanding scaffold loops in triplex origami are also structurally interesting, because they are under a high amount of mechanical strain and can engage in end-to-end oligomerization. We suspect that the base stacking geometry of dsDNA breaks in small loops, but have not investigated the threshold of this phenomenon. Generally, we find that minimizing scaffold bending typically improves the product yield; this is why we designed the triplex sheet with a zigzag scaffold routing, instead of a seamed routing as in the Rothemund sheet.