-The solvent used for post-insertion of the anionophores can also have an impact on the integrity of the liposomal membrane or on the fluorescence of lucigenin. Therefore, the amount of organic solvent added should be rather small: preferably 5 µL is added to 3 mL of liposomes, while 10-20 µL can be added if necessary. While DMSO is commonly used in other transport assays, it cannot be used in the lucigenin assay as it substantially quenches the fluorescence of lucigenin. DMF also gives some quenching, while other solvents (MeOH, THF, acetone, and MeCN) do not cause any significant effect (Figure ). None of the mentioned solvents did cause a higher permeability of the membrane towards chloride ions when used at 0.3% (v/v), see Figure .