The second scheme uses the total reaction energy instead of the QM reactant energy (see Fig. ). In contrast to the first scheme, in this case T13 and T15 show the highest sensitivities compared to the other bases on the 3'-5' strand. This is a result of additionally accounting for the product structure when evaluating reaction energies, since the structure of the two target bases change significantly after the proton transfer. Nevertheless, the bases on the 5'-3' strand again show a higher overall sensitivity, especially base A6.