In addition to identifying hits, the platform enables determination of potency and site of binding, providing actionable information before optimisation of secondary assays and crystallography. Furthermore, hit PhABits can immediately be used as reporters in displacement assays to screen libraries of compounds to identify more potent binders in a site-specific manner. These tools may also prove valuable in qualifying hits from complementary approaches, such as DELs and ASMS. We have also demonstrated their utility for rapid access to PROTAC ligands.