To evaluate human BBB-like characteristic, transendothelial electrical resistances (TEER) were determined with a Millicell ERS-2 voltohmmeter (Millipore, USA) equipped with a STX3 electrode (World Precision Instruments, Germany) prior transport assay. TEER measurements were performed 40 min after medium change. Each model was measured three times, monitoring and excluding a possible data drift due to temperature fluctuations and duplicates were used for each experiment in three independent biological replicates. To yield the TEER values [Ω*cm 2 ] resulted from BBB hiPS-ECs, average TEER of empty inserts coated with collagen IV/fibronectin were subtracted and values were multiplied by insert surface. Results are presented as means ± SEM.