The 3D confocal microscopy measurements were performed using a Leica SP8 confocal microscopy setup (TCS SP8 multiphoton system). The 3D Z-stack imaging was conducted using a 100X oil objective (HC PL APO 100x/1.40-0.70 OIL) and hybrid detectors. The white light laser (WLL) was selected as the excitation source with an excitation wavelength of 488 nm. The Z-stack scan was performed with a line average of 3, an image acquisition speed of 100, and a Z-depth of 100 nm. The electron microscopy experiment was performed with a JSM-7200F field scanning electron microscope.