The Thr residues are supposed to be absent in all the BE-experiments (i.e., the BE-code of [000]) and the signals for T16, T18 and T25 were indeed missing in all the BE spectra. This null code [000] is unique to Ser and Thr residues, thus can provide a good starting point for the sequential assignment. Otherwise, Val and Pro are only residues in Group B (for [010]) whose average Cα chemical shifts in BMRB is >60 ppm. Since in the 1 Hamide-detection experiments Pro residues will not be detected, the downfield-shifted Cα with the BE-code of [010] can be identified as Val with high possibility. Similarly, Ile is the only residue whose average Cα chemical shift is >60 ppm in Group A (for [101]), and Ala is unique with the code of [011]. All these residues (Ser, Thr, Val, Ile and Ala) can serve as a good starting point for the sequential assignments.