The operation of the cartridge is simple, lending itself to automated fluid handling systems. These automated systems could easily be integrated into a bedside design allowing nurses and medical staff to perform a blood draw on patients at different time points after DOX administration and receiving accurate DOX measurements quickly. The speed of applying the DOX quantification analysis directly after blood draw, without needing to physically move the blood sample to the clinical laboratory for processing followed by offsite HPLC analysis, increases the accuracy of the DOX quantification by avoiding DOX degradation that occurs in the unprocessed blood sample after 15 min. In the future, injection molding techniques can be used to create cartridges that have fewer layers and use less material making them smaller and easier to manufacture. This cartridge technique can be applied to additional drugs beyond DOX. The only changes that need to be made to the system are to determine the proper internal standard (as would be required for HPLC analysis) and adjusting the single mobile phase. This process can also be adjusted to accommodate drugs that are not inherently fluorescent. The use of TLC plates coated with F254 allows photon absorption of the drugs to be optically monitored using the same TLC process by looking for blocked fluorescence from the TLC plate. The only difference in the analysis is that the greyscale image of the TLC plate would need to be inverted so the bands would show up as varying degrees of grey against a black background to allow the analysis software to quantify the band fluorescence intensity. Future work will explore the sensitivity of this approach and whether it can be applied to drugs in the physiologically relevant concentration range.