Due to a limited signal to noise (S/N) ratio of resistive-pulse sensing using a 111 nm radius pipette, only liposomes with radii greater than ~50 nm produced quantifiable resistive pulses (Figure ). Liposomes with radii <50 nm produced resistive pulses too small to detect over the signal noise. For liposomes that were larger than 50 nm in radius, the cryoTEM images indicated that 72% are unilamellar, 27% are MV1, and 1% are MV2 (Ntotal =406). There is a slight increase in the degree of lamellarity in larger liposomes.