Dynamic light scattering and zeta potential measurements were performed on either a Malvern Zetasizer Nano ZS or a Malvern Zetasizer Ultra Red Label. Measurements were made at a scattering angle of 173 ° using a He-Ne laser with l = 633 nm. Size-only measurements involved adding 100 µL of sample into single-use low-volume cuvettes, while zeta measurements were performed with Malvern folded capillary zeta cuvettes, where cuvettes were filled to the desired volume specified on the cell. For sample preparation, lyophilized protein was dissolved in a prechilled solvent on ice for a duration of 10 minutes, accompanied by intermittent vortexing to ensure thorough dissolution. To establish the absence of any potential contamination, solvent scattering measurements were conducted prior to the ELP measurement. Solvents were pre-filtered before ELP dissolution using a 0.4 µm syringe filter. ELP DLS measurements were conducted in triplicate for each specified temperature point. Samples equilibrated for 120 s between the incremental temperature shifts of 2 °C before size measurements; there was no equilibration time between the zeta potential and size measurements at a given temperature.