Next, we prepared a ternary model emulsion using SDS, phospholipids and apoB. Since apoB is poorly water soluble, we used LDL as a vehicle introduce this protein to the interface. During homogenization, the LDL particles disintegrate, and the contained apoBs and phospholipids adsorb at the interface of droplets . We labeled the apoB using a secondary apoB antibody conjugated with Alexa Fluor 555. We note that we used an antibody against human apoB, which this however strongly resembling apoB present in egg yolk . Figure indeed shows that this antibody is specific for egg yolk apoB present at droplet interfaces. In the SDS, phospholipid and apoB ternary emulsion a slightly more heterogeneous distribution could be observed compared to the ternary emulsion with phosvitin/phospholipids/SDS at the interface (Figure ). We again applied segmentation to obtain localizations for each droplet in the field of view (Figure ). The analysis of the peaks gave a Arel value of 0.47, indicative of a heterogeneous apoB distribution. In this methodological work, the use of the Arel value to classify model emulsions as homogeneous, partially heterogeneous and heterogeneous is based on the visual assessment of protein distributions at the droplet interfaces. We foresee the use of the Arel value for quantitative modelling of the effect of protein interfacial heterogeneity on physical and chemical stability of food emulsions. This will then also allow further refinement and validation of our proposed classification scheme.