Subcellular localization prediction helps to characterize a protein as a potential drug or vaccine candidate. Cytoplasmic matrix proteins are capable to be selected as potential drug targets and both inner and outer membrane proteins can act as potential vaccine targets . CELLO 2.5 predicted the protein to be localized into the cytoplasm and the result was validated by PSORTb, PSLpred and SOSUIGramN (Table ). The protein was predicted as soluble protein by Protein-Sol. Prediction of signal peptide is important to understand the transport system and cleavage sites of the hypothetical protein. Signal peptide was detected by both PrediSi and SignalP-5.0. However, no transmembrane helices were detected using HMMTOP, TMHMM and SABLE which further emphasizes the protein to be cytoplasmic.