For the quantification of cannabinoids, the assay linearity response was from 1-1000 ng/ml. The QC levels, LLOQ (1 ng/ml), LQC (3 ng/ml), MQC (375 ng/ml) and HQC (750 ng/ml) were evaluated for short gradient LC-vDMS-SIM/MS (See Table ) and LC-MRM/MS (See Table ). For all methods, inter-assay accuracy was in the range of 93.9-114% for all analytes (98.3-115% at LLOQ) and interassay precision was lower than 12.2%. These findings imply that when compared to LC-MRM/MS, the short LC-vDMS-SIM/MS technique performs similarly in terms of linearity, accuracy, and precision.