We previously established that the SF RT approach detailed here leads to Au NPs as or more active than protected NPs, for instance obtained using the widely used Borowskaja-Turkevich-Frens synthesis or PVP . The results above show that the proposed synthesis of SF Au NPs is suitable to produce NMs relevant for size studies at the nanoscale. The trends observed confirm that smaller NPs display a larger ECSA and lead to higher MAs. While this conclusion is maybe not surprisingly, it is important to validate that SF Au NPs are suitable for size effect(s) studies.