Confocal imaging of cells was performed on a Leica SPE or Leica TCS SP8 confocal microscope with HCX PL APO 63×/ 1.40 OIL CS2 objective and two 12-bit photomultipliers. The excitation light was provided by lasers of 488 nm, 552 nm, and 638 nm. Epifluorescence imaging was performed with a Nikon Ti-E inverted epifluorescence microscope, equipped with CFI Plan Apo × 60 oil (NA = 1.4) objective and a Hamamatsu Orca Flash 4 sCMOS camera. The images were recorded using NIS Elements. All the images were processed using ImageJ.