Jolliff, 2002), showed low variance. In Figure , several noteworthy regions emerged: small clusters of potent diaryl-, pyridone-, and indole-core-based inhibitors, as well as a larger cluster of AFN-1252 analogues-all of which exhibited high potency. In the PMI plot shown in Figure , most inhibitors displayed a rod-like tendency, aligning with the fact that the most potent inhibitors shared this shape, although a few displayed a planar, disc-like form. This outcome is unsurprising, given that the FabI substrate is an aliphatic chain that remains elongated in the FAS-II system.