We created an osmotic shock by encapsulating 1000 mM glucose into liposomes and introduc- The budding strategy is suitable for rapid on-chip liposome formation and observation, while we've 262 been struggling with how to collect liposomes in the device and how to produce them in large quan-263 tities. We have tried triggering on-chip budding during the production while collecting liposomes 264 through a bifurcated outlet. However, the flow rate is excessively inhomogeneous at the interface 265 between the oil and aqueous phase due to the viscosity difference, which results in the rupture of 266 liposomes (Movie S11). Thus, we adopted an off-chip budding strategy for mass liposome prepa-267 ration (Figure ).