For synthesis of acceptor 6 (Scheme 4), compound 8 upon triflation using Tf2O, Py in CH2Cl2, followed by C3 inversion gave axial C3-OH compound 16. Further triflation of 16 and displacement using NaN3 as a nucleophile furnished compound 17, in 68% yield over two steps. Next, the removal of benzylidene ring gave desired diol 18 in 85% yield. Regioselective oxidation reaction of 18 of primary alcohol with TEMPO, BAIB and concomitant benzyl ester formation gave the desired oxidized ester acceptor 6 in 79% yield over two steps. the reaction for longer period of time (16 h). We tested several reaction conditions for the coupling between the so formed trisaccharide amine and Cbz protected L-alanine derivative, which was finally achieved best using EDC . HCl and HOBt in CH3CN/DMF to afford Cbz alanine appended trisaccharide 19 in 62% yield over two steps. Finally, Pd(OH)2/C-catalyzed hydrogenolysis and purification using Sephadex G-25 gel furnished target molecule 1 in 98% yield.