New entirely bio-based series of copolymers containing poly(alkyl furanoate) as a hard segment and poly(dilinoleic furanoate) (DLF) as a soft segment with a 70-30 wt% ratio, respectively, were As can be concluded from the literature, enzyme catalysts reach the highest catalytic efficiency in temperatures below 100 °C, however, due to the high melting temperatures of furan-based copolyesters and poor solubility of the final products, it was decided to perform the reaction at higher temperature (140 °C). The immobilized form of CAL-B can still maintain its catalytic reactivity in elevated temperatures even up to 150 °C, which is above the denaturation point of CAL-B protein which was established experimentally to be 62 °C. On the one hand, this is an advantage because the syntheses can be carried out at higher temperatures, which allows for catalyzing a wide range of reactions, but on the other hand, it should be borne in mind that most probably the depletion of enzyme activity, in this case, is faster which directly translates into the limited reusability. Nevertheless, performed synthesis resulted in four different furan-based copolymers with high reaction yields (> 89 %). The aliphatic diols used to create hard segments were α,ω-aliphatic linear diols (α,ω-ALD) possessing two primary hydroxyl groups: 1,6-HDO, 1,8-ODO, 1,10-DDO, 1,12-DDDO where the number of carbon atoms located between the two hydroxyl groups is 6, 8, 10, and 12, respectively.