Combined with their shear-thinning and self-healing properties, these results demonstrated the capacity of developed collagen furan hydrogels to act as a cargo for cell delivery. Moreover, cell morphology (adhesion and spreading) was assessed by fixing the cell-laden hydrogels (hfcMSCs) and staining the actin cytoskeleton with fluorescent phalloidin and nuclei with DAPI. Cell spreading in collagen furan hydrogels was evaluated by measuring the cell area under a microscope. As shown in Figure , hfcMSCs adhered effectively to the three-dimensional hydrogel structure even one day after encapsulation. After 7 days, we observed a noticeable increase in cell adhesion and localized remodeling of the hydrogel, as evidenced by the hfcMSCs significantly spreading into the surrounding material (Figure ). Consequently, the quantified data in Figure substantiates that the cell area had significantly increased.