To determine which class of proteases are responsible for the degradation of our gelatingenipin hydrogels, further tests were performed adding specific protease inhibitors to the supernatant of the P. aeruginosa PAO1 strain. 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) was used as a serine protease inhibitor and ethylenediaminetetraacetic acid (EDTA), as an inhibitor of zinc-metalloproteases. The inhibiting action is summarized in Figure .