This study investigated two wastewater treatment (WWTP) sites in the South West of the UK over a sampling period between 2018-2019 (figure ). The WWTPs serve the city of Bath, with a population equivalent of 120,113 and the town of Keynsham with a population equivalent of 21,247. Both sites are surrounded by agricultural areas and have limited contribution from industrial input (<1%). WWTP Bath does have input from a major hospital in the catchment area. 24-hour composite samplers, set up for flow proportional sampling every 15 minutes, were used to sample screened, but untreated, influent wastewater. Samples were transported on ice to the laboratory for processing.