SEC-MALS was completed on two separate systems (organic and aqueous) to quantify polymer backbone molecular weight and dispersity (Đ) depending on solubility. A 1200 series Agilent GPC was used to run all SEC-MALS. An organic system running on DMF with 50 mM LiBr mobile phase and an aqueous system running on 0.5x PBS (BioShop) with 0.02% (w/v) sodium azide were utilized depending on polymer solubility. The organic system separated copolymers by passing through two Agilent PLgel 5 µm columns in series (10 3 and 10 4 Å, 300 x 7.5 mm). The aqueous system utilized a Superose 12 10/300 GL 11 µm column (300 x 10 mm). Both systems contained a pre-column stainless steel filter with a 2 µm pore size (VWR). Following the columns are an Agilent UV detector, Wyatt Technology miniDAWN TREOS MALS detector, and Agilent 1260 Series differential refractive index (RI) detector. SEC calibration and MALS detector normalization was carried out with polyethylene oxide standards (Agilent). All copolymers were prepared at approximately 2 mg/mL and filtered with a 0.45 µm PTFE filter for organic SEC-MALS and a 0.45 µm nylon filter for aqueous SEC-MALS.