PFBT Pdots were prepared using the nano-precipitation method, where a 5:1 mixture of PFBT (40 µg mL -1 ) and PS-PEG-COOH (8 µg mL -1 ) was dissolved in THF. The THF phase was then quickly stirred into H 2 O with a 1:2 ratio (V/V) while in an ultrasonication bath and then left in the bath for 10 minutes. Afterwards, the THF was left to evaporate in ambient conditions for two days. Pdots with different samples are compared by UV-Vis absorption spectroscopy (Figure ) and dynamic light scattering (DLS) to get similar absorption intensity and particle size, respectively.