To generate aqueous suspensions of degradable nanoparticles, while avoiding early hydrolysis of CKA monomers in water, we carried out the rROPISA process in DMF followed by transfer of the obtained nanoparticles to water via dialysis. This strategy preserves the integrity of CKAs, while maintaining the colloidal properties of the nanoparticles as they transfer to water. This process also makes it possible to obtain nanoparticles that can be degraded by hydrolysis and have a high cytocompatibility towards various healthy cell lines.