In Figures 5E and 5F we wanted to highlight the importance of conducting further research on the dynamics and conformational changes of TLRs. As we mentioned, studies presented to date have mainly focused on TLR3 and TLR4. Very little is known about other receptors, e.g. how the conformational changes occur in individual subunits or how full-length receptors behave in relation to the membrane in which they are immersed. In particular, we would like to know whether the location of the receptor (cell membrane or intracellular compartments) determines the TLRs' dynamics and the subsequent ability to bind the adaptor proteins. Figure illustrates the ultimate goal of studying the Toll-like receptors with the use of computational methods which is to get deep insight into each stage of the receptor functioning. Thus, the challenge is to combine all the information, starting from the recognition of the ligand by the receptor, through the triggering of the signalling cascade, to the immune response.