The luminescent assay was performed as previously described . Briefly, the luminescence of 90 µL sample and 10 µL Kinase-Glo Max reagent (Promega, Madison/Wisconsin, USA) was measured by a Tecan infinite M1000 plate reader. The product formation was calculated from the remaining ATP in the reaction according to formula , where Lum is the average luminescence signal of the reaction sample and Lum is the average luminescence signal of the negative control.