demonstrated that these particles aggregate at high temperatures, leading to an apparent increase in the hydrodynamic radius. It should be noted that there were other reports where similar particles (both gold and silica particles) did not aggregate in water, and their size decreased or remained almost the same with increased temperature. In our case, PNIPAM ligands were relatively small compared to the size of particles, and changes upon crossing LCST had a minor influence on hydrodynamic radius compared to other effects. In the previous report, we showed that PSiFe behaved like rigid spheres, and there was no interdigitation of PNIPAM ligands anchored to neighboring particles. Thus, the aggregation was limited, and the shift of the hydrodynamic radius upon temperature increase was relatively small.