With the optimized conditions in hand, we first evaluated the scope of the reaction with a broad range of alkynes (Scheme 2A). A series of aryl alkynes bearing electron-donating groups and electron withdrawing groups at different positions (ortho, meta, or para) of phenyl rings were well tolerated, delivering the alkynylated products 3ab-3ao in moderate yield (32% to 66%) with good enantioselectivities (82% to 94% ee). Many valuable functional groups, such as N,N-dimethyl (3af), halogen (3ai-3al), cyano (3am), methyl ester (3an), and acetyl (3ao) were compatible. Naphthyl-substituted alkyne could also be employed, giving the desired product 3ap in 59% yield with 92% ee. Thiphene acetylene 2q was also found to be suitable, albeit with lower yield and enantioselectivity. Notably, ethynylferrocene was investigated to give the diferrocenyl alkynylation product 3ar in 58% yield with 82% ee and the absolute configuration was confirmed by an X-ray crystallographic analysis. Several bioactive derivatives, such as estrone, cholesterol, and tocopherol, were well applicable to this transformation, delivering the alkynylation products 3as, 3at and 3au in good yields and enantioselectivities (40%-77% yield, 86-93% ee).