First, the transition from lamellar to inverse hexagonal phase was simulated, secondly, the inverse hexagonal phase was pre-build and tested for stability, measuring the lattice distances to compare to experimental data. The chosen PE lipid for the tests is DOPE which is experimentally well-characterized . The phase diagram of DOPE ranging from 273 K to 319 K, and hydration levels from ~ 2 to 20 water molecules per lipid, displays a small region where the lamellar phase is present, but otherwise, the inverse hexagonal phase is observed, or possibly co-existing with a cubic phase .