Assembly of gold-aptamer-nanoconstructs via this partial nucleic acid complementation method consigns 7 nucleotide bases (5' G GAC ACC 3') at the centre of the aptamer as unbound. This ensures the aptamer will preferentially hybridise with the conserved target enteroviral nucleic acid sequence, thus allowing gold nanoparticles to disaggregate and initiate the signal transduction pathway (Figure & 1Aiii).