All staples encode three 20 nt recognition domains, thereby creating a -120° offset between crossovers. Similar to the rectangle, the crossover density is two crossovers every 60R21N (7 turns). The scaffold repeats eight 60R21N domains before looping, giving an approximate length of 207 nm. This matched well with experimental observations (200 ± 14 nm) and the yield of well-formed structures was again high (78%, n = 77). Many well-formed bundles exhibited some degree of bending, which occurred without discernible consistency in bending angle or placement.