Instead, the general trend is that interactions to carboxyl or acetyl groups get longer overall as acetylation increases. Our interpretation is that the reduced diffusion with increasing acetylation slows the jostling that might dislodge an ion interaction, increasing the dwell time for an ion on the biopolymer. Ion interactions with hydroxyl groups appear to be less impacted by either hydration levels or acetylation, as might be expected given the stronger interactions to carboxyl and acetyl groups.