Dispense plotting (3D printing) of hydrogel scaffolds was conducted at room temperature using a compact bench-top 3D bioprinter (Incredible, Cellink, Sweden) working on the principle of an extrusion-based printer following the preparation described in Scheme S2. The printing speed was set to 600 mm/min and different pressures were applied for different concentrations of hydrogel using a conical nozzle with 0.25 mm inner diameter. In general 24 orthogonal stacks (10x10 mm, 0.25 mm layer height and 1 mm strand-center to strand-center distance) were printed and analyzed with a stereomicroscope SteREO Discovery.V20 (Carl Zeiss Microscopy, Jena, Germany) equipped with a Zeiss icc 5 color camera (5 MP, 12 bit), two lenses (0.63x and 1.5x Plan Apo) and a zoom range up to 20:1. For alginate-A-PPheOzi 15 -A hybrid ink system 30 wt.% of A-PPheOzi 15 -A solution was mixed at 37 °C with a 4 wt.% alginate solution and stored for several hours at 5 °C. The crosslinking of the 24-layered construct was conducted using 10 mM CaCl 2 solution. The crosslinked hybrid scaffold was then incubated in water (Scheme S3) and analyzed via stereomicroscope and rheology.