Figure shows a brief description of experiment steps of sample preparation for analyzing thiosuccinimide hydrolysis isomers. 20 mg/mL ADC1 stock solution was diluted to 1 mg/mL working solution using DI water. 2250 µL of human or CD1 mouse plasma was added into a 5 mL Protein LoBind tube containing 250 µL 1 mg/mL ADC1 working solution. The solution was briefly vortexed, before being split into 300 µL aliquots. The final concentration of ADC1 was 0.1 mg/mL. Three aliquots were incubated at 37°C for 168 h using digital HeatBlock (VWR) then stored at -80°C until use, whereas three other aliquots were immediately stored at -80°C after solution preparation (denoted as 0 h).