Organic solvents used in the study were 99% chloroform (Sisco Research Laboratories Pvt.Ltd) and 99.8% methanol (CH 3 OH) (Loba Chem Pvt. Ltd., India) were used for extraction, 99.9% nhexane (C 6 H 12 ) (Pentokey organy Ltd, India) and 99.5% ethyl acetate (C 4 H 8 O 2 ) (ATICO Medical Pvt. Ltd., India) were used for column packing as eluent and for detection of compounds on TLC plates, silica gel was used for column chromatography used as a stationary phase, pure acetone (CH 3 COCH 3 ) and distilled water for washing materials, sulphuric acid (H 2 SO 4 ), sodium hydroxide (NaOH), hydrochloric acid (HCl), ammonia (NH 3 ), distilled water (H2O), ferric chloride (FeCl 3 ), acetic acid (CH 3 COOH), sodium carbonate (Na 2 CO 3 ) were used to test the phytochemical constituents, and DMSO is used to evaluate the anti-bacterial activities. All the chemicals used are analytical grade from Sam Chem General Trading PLC, Addis Ababa, Ethiopia