Staring from the nWCF conformation with SYN orientation, the simulated outcomes indicate a predominant population of nWCF conformers, with occasional occurrence of WCF structures (see below). Delving into the nWCF structures, it becomes evident that their orientations are not static; instead, the system explores various conformers. Interestingly, dynamical conformational characteristics of UBP predicted by the AMBER forcefield align closely with the results derived from the AMOBA based simulations discussed above.