As will be shown in results, two specific residues, W238 and F241, were predicted to be important for membrane association for DHDDS to the membrane. To determine the role played by these residues in protein-membrane interactions, the W238AF241A double mutant was developed in psfgen. From the same starting pose as the ligandpresent simulations (the end of replica 3 from the initial apo simulations), 5 simulation replicates were run to 1 µs to assess the impact on binding and dynamics for h-cisPT.