Another promising solution for replacing PCR amplification is Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas. CRISPR/CAS is a complex immune system in archaea and bacteria protecting them against foreign virus nucleic acids, which is considered to be the most significant discovery in the recent year in biology . In brief, it is based on the ability of the bacteria to store fragments of foreign DNA in the CRISPR loci as a memory, that together with surrounding repeats, are later used by the Cas enzymes to recognise the invader and inactivate it. CRISPR/Cas system can be classified based on the specific Cas enzymes and the interference mechanism. There are two main classes according to the interference mechanisms and class 2 comprising three different enzyme types (Cas9, Cas12 and Cas13) is most often applied in nucleic acid-based biosensors . For instance, CRISPR/Cas system was recently applied for the detection of whole bacterial cells by and .