Cyanamide-driven dry-state condensation of phosphatidic acids with ethanolamine and choline, or diacylglycerols with phosphoethanolamine, have been shown to provide phosphatidylethanolamines and phosphatidylcholines (Fig. ). It has also been demonstrated that solution-phase transacylation of Nacyl imidazoles and glycero-3-phosphocholine, or acyl thioesters and lysophosphatidylcholines, result in the formation of phosphatidylcholines (Fig. ). Nonetheless, these processes rely on substrates not yet shown to have been prebiotically available (choline, glycerol-3-phosphocholine, and lysophosphatidylcholine), and require activating agents and conditions that inevitably lead to byproduct formation. Moreover, to date, no bottomup prebiotic study has addressed the predominance of 2-amino alcohol motifs, nor the universal occurrence of sn-glycerol-3phosphodiesters (or their enantiomers, sn-glycerol-1-phosphodiesters), in complex (glycero)phospholipids.