The optical trapping and single particle tracking (SPT) experiments were conducted using a conventional widefield setup. A schematic of the optical setup is illustrated in Figure . A 488 nm laser (100 mW, Spectra-Physics) was selected as the widefield excitation source. The widefield excitation laser was focused on the back aperture of an objective lens (NA = 0.90, Olympus UPLFLN 60X Objective) via a widefield lens. The emission light passed through a set of lenses in a 4f configuration and a proprietary prism enabling multiplane imaging. The different imaging planes were collected by two sCMOS cameras, with four image planes each. For the optical trapping experiment, the NIR laser 1064 nm (Laser Quantum Opus 1064, UK) was used. The 1064 nm laser was focused at the same back aperture of the objective lens by passing through a beam expander and mirrors.