A notable study by Liu et. al illustrates the application of the Single-probe SCMS experiment combined with SinCHet-MS (Single Cell Heterogeneity for Mass Spectrometry) software to investigate tumor cell heterogeneity and cellular subpopulations . They analyzed the metabolomic profiles of drug-sensitive and drug-resistant melanoma cells (WM115 and WM266-4) treated with vemurafenib. The data was subjected to batch effect correction, subpopulation analysis, and biomarker prioritization. Notably, the findings showed that drug-sensitive cells developed a new subpopulation after treatment, while drug-resistant cells only showed changes in existing subpopulation proportions. There are a few highlights of this work.