To evaluate the effect of milling on cannabinoid measurement accuracy, we measured the cannabinoids content of extracts from cannabis milled under various condi`ons. Using two established milling tools, a coffee grinder and a food processor, as well as a single-use milling container, THCA and CBDA cannabis flower was milled to different protocols. This study aims to fill the gap in knowledge regarding how different milling methods affect analyte recovery in cannabis or related plant extracts. under varied milling conditions (milling speed and time). The resulting particles were sized and extracted using HPLC grade methanol using sonication assistance. The filtered and diluted extracts were analyzed via HPLC-VWD to quantify cannabinoids.