Looking towards the future, when the integration of reference electrodes into microfluidic MFCs is more straightforward, we anticipate new studies that include EIS and voltammetry to more deeply investigate overshoot. Based on our observations, we believe that it is not the MFC health state that causes the power overshoot but rather the related slower electron transfer kinetics that result in the polarization tests being conducted before EAB was adequately stable. This is proven by the ability to eliminate overshoot and even improve on MFC outputs over the initial (healthy) measurements using a long-hold technique on a health-compromised MFC. The reader is directed to the Supplementary Materials (Section S9) where we have summarized the terminology, conclusions and guidelines on proper conduction of polarization tests.