The study investigated the effect of age on the degradation of silymarin by dividing the donors into two groups: healthy young (aged 12-45 years, n = 10) and healthy elders (aged 70-80 years, n = 10). PERMANOVA showed marginally significant values for the effect of age on silymarin catabolism [R 2 = 0.117, Pr (> F) = 0.055] when analysing the LC-MS data. Univariate paired comparison based on AUC revealed that the unidentified metabolite 399.105@10.4 was higher in healthy elders (p = 0.0030, q = 0.0670). A few additional metabolites showed differences prior to the FDR correction, all of which were more abundant in healthy elder subjects (Fig. ). The abundance of catabolite 399.105@10.4 was 2.5 times higher in the group of healthy elders compared to the healthy young group.