, which is consistent with the numerical simulation results. Thus, we concluded that the timingcontrolled division of DNA droplets was achieved using a time-delay circuit. Next, we applied the time-delay circuit to control the pathway of DNA droplet division (Figure ). We used a ternary-mixed C•A•B-droplet, comprising three types of Y-shaped branched DNA nanostructures (YC, YA, and YB) connected with two types of linkers (L † AC and L † AB) (Figure ). YA, YB, and L † AB are the same as those used in the previously described experiment; L † AC was designed to crosslink YC and YA. From the viewpoint of the reaction landscape shown in Figure ). This indicates that Pathway 1 was selected via the inhibition of T † ABi due to the presence of R † ABi (miR-6875-5p and miR-4634). Next, to achieve Pathway 2, we added active triggers T † ABi (for cleaving L † AB earlier); inhibited triggers iT † ACi, excess inhibitors R † ACi, and RNase H (for cleaving L † AC later). For R † ACi, miRNA sequences, miR-1246 and miR-1307-3p, were used (Supplemental Table ). Supplemental Movie S7 shows that the order of the division of B-and C-droplets was also controlled well. The C•A•B-droplets were first divided into B-droplets and C•Adroplets approximately 30 min after the addition (Figure ). After another 20 min, the C•A-droplets were divided into A-and C-droplets (Figure (ii)). This indicates that Pathway 2 was selected because of the presence of R † ACi (miR-1246 and miR-1307-3p). Thus, the pathway-controlled division was achieved using time-delay circuits.