We used LPS stimulation and a drug-induced acute liver toxicity model using an N-acetyl-paminophenol (APAP), which is also the most common etiology of drug-induced liver injury. Given the limited efficacy of iBETBD2 in cellular studies, we initially focused our analysis on I-BET151, 1, 2, and 3. In the first experiment, mice were given 30 mg/kg inhibitor orally (PO) or vehicle before injection of LPS to induce inflammation (Figure ). Liver samples were isolated to assess chemokine expression. In the LPS-induced model, only I-BET151 and 3 induced a significant reduction in expression of the inflammatory chemokines Cxcl1, Cxcl2, and Ccl2. A reduction in chemokine expression from 1 and 2 was observed but was not significant.