Prior to the product purification using flash column chromatography, the optimal eluent composition is typically determined with manual TLC. TLC fine-tunes the eluent polarity to ensure that the retention factor value (Rf value) of the target compound falls within 0.2-0.3, and, at the same time, impurities are separated from the target compound. A recent publication has applied machine learning model to predict the Rf value of a given compound structure in different eluent compositions .