The implemented exponentially decreasing crossflow profile provides similarly high initial crossflow rates combined with a decreased averaged flow rate. These averaged flow rates are too low to accomplish sufficient separation since the particle positions in the separation channel are not sufficiently different, resulting in co-elution of AuNP and C/S particles and resolutions of 0.9 (for 0.4 mL/min) to 1.1 (for 0.6 mL/min). While recovery suffered from the change in the crossflow profile, the resolution did not improve significantly. The decision to use a specific separation method is based on the needs originating from the desired application of C/S particles after purification. While the highest possible resolution is chosen for ultrapure particles, high recoveries are necessary if high particle concentrations are needed. Since, in our case, both criteria are relevant, we decided to find a compromise between resolution and recovery. With a linearly decreasing crossflow profile and an initial flow rate of 0.5 mL/min, we obtain a sufficient resolution of 1.2 and a good recovery of 79%.