The Single-probe SCMS technique has significantly advanced semi-quantitative single-cell metabolomics by enabling precise, real-time analysis of individual cells across diverse biological systems. Its applications cover multiple areas such as marine microorganisms, human diseases, and cell-cell communication, offering unprecedented insight into cellular heterogeneity and metabolic dynamics. As the technique continues to evolve, it holds immense potential for furthering our understanding of complex biological processes and driving innovations in personalized medicine.