The employment of redox proteins, such as Cyt c, frequently necessitates immobilisation onto a material substrate. Nanomaterials, due to their extensive potential for applications across diverse fields, several of which intersect with those of Cyt c, are increasingly drawing the attention of the scientific community. Thus, the incidence of Cyt c immobilisation on nanomaterials is not an infrequent activity . However, while immobilisation is often reported, there is a discernible lack of discussion surrounding the potential implications that this environment may have on the redox capabilities of Cyt c. It is crucial to investigate this domain, as alterations in redox functionality could critically affect the protein's applicability in various use cases.