So far, we have applied PCVA for QM region construction to protein systems. In the following, we will assess whether this approach can also be used for a DNA system to quantify and evaluate the effect of single bases on properties of the QM region. Instead of amino acids here the sensitivities of single (nucleo)bases are target of the analysis, which is why we will refer to single base PCVA (sbPCVA) in this specific case.