Yang et al. reported a rapid paper-based detection system for determination of human serum albumin concentration. This study introduces a new analytical platform for detecting human serum albumin (HSA) concentration, composed of a paper-based chip (µPB-Chip) and a small analytic box with a heating system and cell phone camera. The concentration of HSA is determined by adding a Bromocresol Green (BCG) reagent to the reaction area of the µPB-Chip and measuring the intensity of the resulting albumin-BCG complex after a 12-minute reaction at 37°C, using the R (red) value. The platform is tested with albumin concentrations ranging from 0 to 5 g/dL in HSA control samples and is found to be consistent with conventional assay techniques (R2 = 0.9912) when tested with 36 volunteer patient samples. Compared to traditional methods, this platform offers simpler experimental procedures, shorter detection time, and the ability to perform the entire reaction/detection process on a single integrated platform. Additionally, the results can be wirelessly transferred to a remote data center, making this platform a convenient, compact, reliable, and economical method for measuring HSA concentration.