For the bases it can be seen that in general the binding free energy scales approximately with base strength i.e. the binding free energy goes as follows: EDA < TMA < DMA < MA < AM. For the acids, a trend is less clear. It can be seen that IsA and IT exhibits lower binding free energies with the acids (-11.7 to -3.2 kcal/mol) compared to IA and IP (-9.6 to -1.0 kcal/mol). This can be attributed to the binding pattern observed earlier, where IsA and IT favoured hydrogen bonding while IA and IP favoured halogen bonding.