The amino acid sequences corresponding to the F13L from the 2022 multinational outbreak of MPXV infection corresponding to isolate clade IIb lineage B.1 (containing the E353K mutation) , was downloaded from GenBank URK20480. The F13L amino acid sequence was alphafold modeled (F13L0) (), obtaining a Root Mean Square Deviation (RMSD) of 9.6 Å with the crystallographic model of phospholipase D of Streptomyces sp (ID 1v0y) at ) . The F13L coding for pools of the most abundant Tecovirimat-resistant mutations (Table and Figure red solid circles) were introduced into their amino acid sequences before being uploaded to Alphafold. The five alphafold models obtained in each case predicted minimal RMSD of ~ 0.2 Å differences among them. Therefore, the corresponding number 1 models were selected for the computational studies. The new mutants contained a pool of the common E353K and the most abundant Tecovirimat-resistant mutations as follows, mutant F13L1 (mutations E353K+N267D+ A288P+ A290V+ D294V+ A295E+ I372N) and mutant F13L2 (mutations E353K+ N267deleted+ A288P+ A290V+ D294V+ A295E+ I372N). The F13L2 alphafold model required one additional "clean geometry" step by Discovery Studio, to relax their predicted 3D structure as required for DWBEL, most probably due to the inclusion of the N267 deletion.