For the Gx-psDiMan binding studies, the G5-psDiMan (100% and 50%) buffer was exchanged 3 times with the post-dialysis binding buffer using a 10 kDa cut-off centrifugal concentrator to obtain a final concentration of 300 nM. The G13-psDiMan buffer was similarly exchanged with the post-dialysis buffer using a 30 kDa cut-off centrifugal concentrator to obtain a final concentration of 100 nM. The DC-SIGN concentration used here was 30 µM. The DC-SIGN solution was loaded into the syringe, and Gx-psDiMan solution was loaded into the calorimeter cell. A titration of DC-SIGN into buffer was performed as a control titration. Enthalpy changes of Gx-psDiMan binding to DC-SIGN were obtained by subtracting the average of the last 4-8 data points of the control titration which have similar heat changes to correct the effect of heat dilution. The titration curve was fitted with the same method described above to obtain ∆H o values.