Finally, the adsorption of 20 bp dsDNA oligonucleotides and pDNA into PDMAEMA brushes was investigated via neutron reflectometry. Comparable changes in the reflectivity traces were observed, compared to those observed for RNA infiltration (compare Figures and). However, the shifts in the RQ 4 vs Q minima were not as pronounced and the structure of the profiles in the mid-Q range following DNA adsorption differed compared to that of RNA molecules. Associated with these changes, the parameters used to fit these profiles reflected changes primarily within the deeper layers of the brush (Table ), with overall little impact on the total swollen brush thickness, in good agreement with ellipsometry data. Analysis of the SLD profiles indicated relatively deep infiltration of DNA oligos, in particular after injection of 10 µg/mL solutions, whereas pDNA was found to be distributed also in upper layers of the corresponding complexes (Figures and).