Mass spectrometry analysis: SCREEN samples were separated via online nano LC on a Dionex UltiMate Tandem 3000 UHPLC instrument. All samples were loaded for 5-mins at 5-µl per minute to PhamaFluidics trapping column with 100% of buffer A (0.1% formic acid in water). A constant flow rate of 200nl/min was used during separation gradient of 90 min. After sample loading and port valve switch to elute samples into PharmaFluidics 50 cm analytical columns on HPLC active gradient ramped from 0-5% B buffer (0.1 % formic acid in acetonitrile) in 5 min followed by linear part of the gradient from 5 to 30 % B buffer over 60 mins gradient then ramped to 98% B buffer over 10 mins and stayed at that level for 15 mins of the active gradient. During injection of single cell sample on analytical column 1, second trapping column and second analytical column on tandem UltiMate 3000 pump underwent wash gradient for the first 3-50 mins followed by the next step with the last 20 minute of equilibration procedure with 98 % A as a preparation step for next injection cycle. All samples were analyzed by a Thermo Scientific Q-Exactive HFX mass spectrometer from 20 to 90 minutes of the nano HPLC gradient. Trial samples that contained only carrier channel were run on Tribrid Lumos Orbitrap instrument to compare identification rates with and without help of ions trap MS2 scans.