The 2D-FES has been computed along the difference between the distance between the center of geometry of the macrocycle and the center of geometry of one stopper and the distance between the center of geometry of the macrocycle and the second stopper (d ). The rates for the decomplexation of [10]CPP-Fullerene Pseudorotaxane 21 have been calculated running 20 infrequent WT-MetaD using the distance between the center of geometry of [10]CPP and the center of geometry of the fullerene as CV, depositing Gaussians every 40 ps, 0.5 kJ/mol of initial height, 0.1 nm width and a bias factor of 10.