They assumed that it is possible that the negatively charged dsRNA restricted the insertion of LRR-NT into the membrane surface. During the simulations, the dsRNA kept its structural integrity while bound to TLR3. The observed distortions in the TLR3-TM domain were distinct from the previously reported TLR4-TM. Thus, the authors concluded that the orientation and conformational changes of each TLR type may vary, depending on their location in the cell or the lipid composition in the membrane. Based on the MD simulations analysis, Patra et al.