Isolation of EVs from IM-HCEpiC. IM-HCEpiC were cultured in collagen coated flasks until a large number of cells were achieved (passage 4-7) to be able to obtain a volume of 50-500 mL collected media with EVs. Opti-MEM (0.1 mL/cm 2 ) was added to the IM-HCEpiC when they reached ~80% confluency. The cells were incubated with Opti-MEM for 48h and then the supernatant was collected and centrifuged (Eppendorf Centrifuge 5810 R) at 700 RCF for 5 min at 11°C. The new supernatant was collected and centrifuged at 1450-2000 RCF for 10-15 min (Eppendorf Centrifuge 5810 R). The supernatant was then filtered through a 0.22 µm syringe filter, the final volume was noted down and a sample for NTA (1000µL) was collected and stored in the fridge.