To further demonstrate the robustness of Cstar self-assembly to topology-preserving design changes, we produced a C-star variant featuring a responsive motif. Relying on toeholdmediated strand displacement, we can induce the isothermal melting of these modified C-star frameworks upon exposure to a trigger oligonucleotide. The potential of strand displacement reactions as a means of controlling structural rearrangements in materials has been demonstrated in arrays of DNA-functionalized gold nanoparticles, 50 colloids, and liposomes. However, previous attempts to program a triggered response in all-DNA crystals have been limited to the grafting/displacement of fluorescent DNA oligos, producing color changes but no structural response. Figure shows a schematic of the implemented strand displacement mechanism, leading to dissolution of the C-star network. This C-star variant is designed such that the core strands (blue in Fig. ) are not bound directly to the cholesterol-functionalized strands (orange in Fig. ). Instead, a bridge strand (green in Fig. ) connects the nanostar core to the cholesterolized strands, while presenting a dangling 6 nucleotide toe-hold. Upon the addition of the trigger strand (red in Fig. ), which is fully complementary to the bridge strand, the core and cholesterolized strands are displaced leaving cholesterol-DNA micelles, free nanostars, and a double stranded waste product composed of the fully hybridized trigger and bridge strands (bottom-left panel, Fig. ). The correct functionality of the triggered disassembly mechanism was demonstrated in freely diffusing nanostars using DLS and a fluorescence quenching assay (see SI text and Fig. ).