The diblock copolymer PEGMA17-b-DEAEMA27 was targeted at 10000 g/mole with 70:30 wt% composition of DEAEMA to PEGMA. It was synthesised via sequential GTP. The exothermic polymerization reaction was monitored using a digital thermometer attached to the bottom of the flask. The exothermic polymerization reaction meant that the temperature increased while the monomer was polymerizing, and it started dropping when all the monomer had converted to the polymer, thus allowing the monitoring of the sequential polymerization. First, 10 mg of TBABB was added into a 250 mL round bottom flask filled with Argon, then 62.88 mL anhydrous THF was injected into the flask. Followed by injection of 0.5 mL freshly distilled MTS. Then the first monomer was added, 23.44 mL PEGMA (23.44mL in 50v/v% in THF, 12.31g, 0.041 mol). The temperature started to increase from 21.5 to 31.5°C. When the polymerization of the first monomer was completed, two samples were taken out from the reaction flask for GPC and 1 H NMR. Then DEAEMA was added (13.35 mL, 12.31g, 0.066), whereupon the temperature increased again from 23.8 to 34.9°C; two 0.1 ml samples were then taken for GPC and 1 H NMR. As GTP is highly moisturesensitive, the flask was kept sealed by a rubber septum and purged with inert argon gas each time after materials were added or taken. After the polymerisation was complete, polymer was transferred to hexane drop by drop for precipitation, to remove any undesired low MM molecules and catalyst. Solvents were then removed, and the precipitated polymers left in a vacuum oven to dry at room temperature for at least a week. The polymers were then collected into vials; liquid nitrogen cooling was used to aid this transfer.