asiatica, which will be used for further investigations in drug discovery. To aid this process, the accumulation rates of these triterpenoids, and whether they exist as free acidic (aglycones) or as glycosidic (glycosides) forms as well as their relative ratios during the time in the year were also the subjects of our study. For these purposes, the raw ethanol-water extract was refluxed with aqueous NaOH solution for the hydrolysis of the triterpene glycosides. After hydrolysis, the solution was acidified, causing the free acidic triterpenes to precipitate or to enable extraction with ethyl acetate . The HPLC analysis of the raw ethanol extract before hydrolysis and the obtained solid or ethyl acetate extract after hydrolysis will provide information on the accumulation ratios and glycosylation states of the triterpenoids during the year, which will aid those who require particular examples for biomedical studies.