Fast protein liquid chromatography (FPLC) was carried out on an Äktaavant (GE Healthcare, Chalfont St. Giles, Great Britain) to analyze resulting PSar conjugates. A GL 10/300 Column (300 mm, Ø 10 mm, GE Healthcare) was packed with Superdex 75 resin under constant pressure. The purification was conducted in 10mM TRIS buffer with 300 mM of NaCl (pH = 8.0) with a linear flow of 0.5 mL/min.