In summary, we have studied the binding kinetics of YOYO-1 dye to the immobilized DNA molecules and measured the adsorption cross-sectional of the probe-target interaction based on a recently developed mathematical model. Our equation is indeed useful for predicting the binding kinetics of probe-target interaction from the adsorption cross-section of the binding, the concentration of the probes, and the diffusion coefficient of the probes. The adsorption cross-section is remarkably consistent with the physical binding area with a concentration dependence for the example case of YOYO-1 DNA binding. Even if we don't know the concentration dependence of the biding and assume a random adsorption cross-section between boundaries, our model yields the binding rate that is remarkably accurate within a few folds of magnitude, compared to the several orders of magnitude discrepancies in typical calculations using previous models . With measured adsorption/absorption cross-section for YOYO-1 DNA binding, we can now predict the binding rate at >90% accuracy. If this method is generalized for typical bindings from calculating the adsorption cross-sections and combining with the dissociation rate constant that can be measured or calculated, we can now predict the key parameter IC50 in drug discovery, which is the concentration of the probes at which the adsorption rate equals the desorption rate and about 50% of the target molecules are occupied by the probes.