These findings contrast with those from the ASR of the thermophilic myo-inositol-3-phosphate synthase family proposed to have descended from a thermophilic archaeal ancestor, where the resurrected ancestral forms were more stable than the extant enzymes . However, in both cases, the trends in thermostability in the inferred ancestors vs. their extant counterparts reflect the expected changes based on the hypothesised niche of the ancestral organism; here, the ancestor is presumed to have been a mesophile since the majority of the extant forms containing a CYP116B are mesophilic, whereas the ancestral myo-inositol-3phosphate synthase is proposed to have existed in an organism that was more thermophilic than its descendants.