The extent of the irreversible inactivation of SARS-CoV-2 by CV-N after washout was determined using the previously described infection inhibition assay. HEK-293T/ACE2*cl.13 cells were seeded at 10,000 cells per well in a 96 well plate and incubated overnight. "CV-Ntreated" SARS-CoV-2 pseudovirus samples were created by mixing produced SARS-CoV pseudoviruses (50 ng of p24 worth of pseudovirus) and CV-N at various concentrations, incubating at 37°C for 2 hours, then washing and resuspending the virus 10 times in fresh media 100kDa spin concentrators to remove unbound CV-N. "CV-N-treated" SARS-CoV-2 pseudovirus samples were then added to the HEK-293T/ACE2*cl.13 cells for 24 hour incubation, followed by a media change, and then another 24 hour incubation. Infection was then measured via the NanoLuc reporter as described in the Infection Inhibition Assay method. Wash flowthroughs and recovered viruses (both SARS-CoV-2 pseudoviruses and Spikenegative virus-like particles) were tested for removed and virus-retained CV-N by gel filtration and Western Blot.