In our vision of de novo synthesis of life, self-division is critical. Nevertheless, equally important is that the offspring droplets can become the next generation of droplets upon refueling (Fig. ). In this context, a speckle liberated in the form of an offspring droplet should serve as a nucleus for the growth when fuel is available again while suppressing the nucleation of new droplets. We refer to such a droplet as a second-generation droplet. Thus, experimentally, the exact timing of the refueling is critical. Too early in the cycle leads to a regrowth of all mother droplets, and too late results in the complete dissolution of all droplets and a reset of the system (see Fig. ). We tested the refueling experiments using 12.3 mM peptide, 20 mM PSS with 75% short PSS, and 25% long PSS fueled with 50 mM EDC. The first coacervate droplets could be observed 30 seconds after the first fuel addition (Fig. ). The droplets grew and fused for several minutes. After roughly eleven minutes, the vacuolization started, followed by the speckle formation and the liberation into offspring. After 11 minutes, we added a second batch of 50 mM fuel. Because of the flow induced by adding a second batch of fuel, it could be hard to track the offspring droplets. We tracked a few droplets and found that their size increased quickly after adding fuel, e.g., from 1 to 1* in Fig. , and more examples in Fig. . 30 seconds after the second fuel addition, we observed a bigger and smaller population of droplets in the sample. We assume that the bigger ones originated from the offspring droplets and thus are second-generation droplets. The smaller droplets were freshly nucleated, the new first generation. We found the resulting second-generation droplet to be more viscous in the first few minutes than the first-generation. The fusion with other droplets is slower, resulting in non-spherical droplets. Over time, the second-generation droplet takes up more short PSS, resulting in a less viscous droplet that fuses more easily. Towards the end, the vacuole formation, followed by the speckles formation, is observed. Finally, the coacervate droplets' division occurs (Fig. , supporting movie 2). We conclude that we can rescue the offspring by adding a second batch of fuel. In addition, the division mechanism is not affected by the second fuel addition nor by the different morphology of the second-generation droplets due to the initially higher long PSS content. Rescuing the offspring droplets with another batch of fuel is exciting, as it means that the identity of the droplets is not wiped out with every refueling experiment. Ideally, information-containing molecules (like a synthetic genotype) are passed on from the mother to the offspring. As our system does not contain any such genotype, we tested whether such experiments are positive with labeled, positively charged peptide (Cy5-R30) as a model. The R30 is homogenously distributed in the coacervate during the reaction cycle. Towards the end of the cycle, the R30 remains in the offspring droplets until they eventually dissolve (Fig. ). In other words, positively charged molecules like R30 are passed from the mother droplet to the offspring.