Cell Culture and Immunoblotting: HEK293T cells were maintained in DMEM supplemented with glutamine, penicillin, streptomycin, and fetal bovine serum (Seradigm). Flag DNAJB8 H31Q plasmid has been reported previously . Flag GAPDH in the pCMV3 vector was purchased from Sino Biological. Immunoblots were transferred to nitrocellulose from SDS-PAGE gels using a Bio-Rad Trans-Blot Turbo, stained with ponceau S to image total protein, blocked with 5% dried milk/TBST (10 mM Tris pH 7.4, 150 mM NaCl, 0.1% Tween), washed with TBST, incubated with primary antibody in 5% BSA/TBS with 0.1% NaN3, washed with TBST, incubated with near-IR conjugated secondary antibody in 5% dried milk/TBST, washed with TBST, washed with TBS, and imaged on a Li-COR Fc.