In order to characterize (presence of phases, size, and strain) the Gold (Au) and sugar of milk mixture during mechanical grinding, XRD analysis has been performed. Figure shows the XRD pattern of the powder sample with different concentrations of Au and sugar of milk. The diffraction peaks confirm the presence of Au NPs with lactose (sugar of milk) peaks. The peak at 38° corresponds to the (111) plane of Au nanoparticles and matches with the standard JCPDS card . Along with the Au NPs peak, we also observe other peaks corresponding to the lactose. We do not observe any other impurity peaks.