As phase transitions can be challenging to quantify (especially in systems with significant hysteresis) , we also explored seeding simulations (Figure ,B,D) as an alternative approach to determine the Tm of bilayers. To create a seeding setup, we construct a starting configuration consisting of a lipid gel phase surrounded by a liquid bilayer utilizing our newly developed system builder COBY 116 (available on GitHub ), and initialize a series of constant temperature simulations, allowing the system to equilibrate at each temperature (see Methods