Since this site is the exit spot for the zinc ion, blocking it can inhibit zinc efflux. The ZnT-ligand interactions were computed using the Protein Ligands Interactions Profiler (PLIP) server, and it the Protein Ligands Interactions Profiler (PLIP) server, and it was found that Ligand IV showed the strongest interactions. Ligand 1 formed three hydrophobic interactions with His137, Leu141, and Leu142 at a distance of 2.35 Å, 3.62 Å, and 3.82 Å, respectively. In addition, it also formed three hydrogen bonds with His137, Arg140, and Leu141 at a distance of 2.28 Å, Ligand IV forms seven hydrophobic interactions with Asn60, Leu64, Arg129, Pro134, His137, Arg140, and Leu141 at a distance of 3.61, 3.90, 3.77, 3.88, 3.38, 3.70, and 3.33 Å, respectively. Ligand V forms only three hydrophobic interactions with Leu62, His137, and Leu141 at a distance of 3.92, 3.74, and 3.68 Å, respectively. : ZnT-ligand interactions were calculated using the PLIP web server.