In the ZULF spectrum of the hyperpolarized sample HP2, peaks can be clearly identified at 1 J CH = 194.7 Hz for formate and there is one peak at 3/2 × 2 J CH = 8.0 Hz for glycine (see Fig. ). The expected signals of pyruvate at 3 J CH = 1.4 Hz and 2 × 3 J CH = 2.8 Hz are not visible in the spectrum. The formate concentration in the dissolved sample was determined to be 38 mM. Compared to prepolarization at 2 T, the sodium formate peak shows a spin-polarization enhancement of ε = 11000 (concentration ratio of 137 and signal enhancement of ε = 83).