Figure shows the structure of representative activity cliffs in DS, for all TF's, it is evident the relation of agonist and antagonist molecules, supported by previous analysis. Regarding LasR agonist, it is possible to see a change of EC 50 value by a variation in one-carbon of a lateral chain, BO1 has eleven carbons, while CH202 has ten carbons, but the potency decreases in ten orders of magnitude; furthermore, natural autoinducer possesses a lateral chain with twelve carbons; all of these allow us to understand the importance of size for the agonist activity. The molecular pair found for LasR antagonist, MA3 and CH184, differ between them in the final halogen, MA3 has chlorine while CH184 has bromine; but antagonist activity for CH184 is a ten percent respect to MA3; relating the size of the atoms in a chain with the activity; a smaller atomic size generates more antagonist activity.