In experimental studies, the number of molecules, or the number of channels, involved in charge transport is generally very hard to estimate. In single molecular junction like STM-BJ or MCBJ, the single molecule conductance was identified by statistical conductance peak. While in selfassembled monolayer (SAM) based junction, the active molecules under top electrode in the junction measurement can only be approximately estimated, which varied from 10 2 to 10 5 depending on the nature and contact geometry of the top electrode. In model fitting, the number of active molecules in the junction (or the channels) in the transport, was generally treated as a linear multiplication coefficient in front of the I-V response function. This is, a & b Eq 1 and Eq 3 respectively used for fitting in original literature work apparently, a very rough approximation. The multimolecule effect in the junction were explored and it was found the conductance is not a simple linear function of the number of molecules and the interaction between molecules can make significant difference .Nevertheless, in model fitting, it is quite common to put the guesstimated number of the molecules in front of the model as multiplication coefficient, which are