Gramicidin A is an ion-channel-forming peptide, selective for small ions, such as protons. Under ambient aerated conditions, the initial quantum yield of gramicidin A was reduced and performed better in an inert atmosphere compared to the same system without gramicidin A. It was observed that gramicidin A played a role as an ion balancer, leading to an increase in initial quantum yield and an improvement in reaction rate. Gramicidin A accelerated the electron transfer rate constant by 11 % under Ar and 27 % under air. The quantum yield in the first 10 min is increased by 20 % under Ar and by 50 % under air. Another transmembrane ion transfer reagent 18-crown-6 ether was incorporated into the membrane. This transporter is selective only to K + cations. Adding 18-crown-6 to the phosphate buffer in the experiment enables the transport of K + cations to compensate for the charge imbalance in both compartments. It was observed that the addition of 18-crown-6 did not result in a better rate or quantum yield of transmembrane transfer compared to the addition of gramicidin A.