To increase the PF from 0 to 1, SSA the strength of membrane association events are shown to decrease. However, for an SSA to demonstrate membrane permeation properties (PF ≥ 1), and for those properties to be further enhanced, a decrease in MAF is required which correlates to enhanced molecular membrane adhesion properties. We hypothesise that to achieve PF ≥ 1, the SSAs may have to form extended self-associated structures similar to the membrane pores or channels reported by Hou and et al.. The increased strength of SSA membrane adhesion events leading to enhanced SSA membrane permeation properties supports the formation of stable membrane bound structures, that facilitate the flow of SSAs through the phospholipid bilayer, increasing the PF.