IMAC fractions were resolved on 10% SDS-PAGE gels and stained with Simplyblue SafeStain (Invitrogen) or Coomassie Brilliant Blue. For western blots, gels were transferred to PVDF membranes (20 V, overnight). Membranes were then blocked (TBS-T w/ 5% BSA; 1 h) and probed with primary antibody (4°C overnight) on an orbital rotator. Blots were then washed (3 x 5 min. with TBS-T) incubated with secondary antibody (1h; room temp.). Blots were then washed (3 x 5 min. with TBS-T) and rinsed briefly with distilled, deionized water prior to addition of chemiluminescent substrate. Chemiluminescent images were captured using an Azure c300 imaging system.