In our previous work with DDR1, 14 pairwise distances were hand-picked based on prior knowledge of the system. In contrast, in this work, we employed a semi-automated approach to select these CVs. Since DFG flipping was one of the conformational changes we aimed to observe, we included a few sidechain atoms known to be associated with DFG flipping, along with all the C α atoms from the activation loop and nearby regions (See Supplementary Information). This selection resulted in 11 pairwise distances, two of which involved the terminal ζ-carbon of the DFG-phenylalanine. These two CVs are similar, but not identical to the Dunbrack distance, which is a common CV used to monitor DFG flipping. This set of CVs successfully captured both the A-loop movement and the DFG flipping. However, it should be noted that while our protocol automatically selected these 11 pairwise distances, the scope of atom selection was still tailored to our specific goal of identifying alternative A-loop and DFG states.