Bubble induction, endocytosis, and infection of macropinocytosis rely heavily on the presence of exposed phosphatidylserine in the viral membrane . This protein kinase 3 is a serine/threonine kinase that phosphorylates and oligomerizes mixed lineage kinase domain (MLKL) proteins upon activation . Oligomeric MLKL creates membrane pores that permit the release of hazard-related molecules . Multiple phosphatidylinositol 3-kinases regulate vaccinia virus morphogenesis and mediate poxvirus entry . Integrin 1 facilitates the entry of the vaccinia virus by activating PI3K/Akt signaling . Early activation of the PI3K/Akt pathway is required for host survival and viral replication during vaccinia and vaccinia virus infection . Evolutionarily similar to class I PI3K, type III phosphatidylinositol 4-kinase beta isoform (PI4KB) has been identified as a key host factor mediating the replication of numerous destructive pathogenic virus . For example, the role of endocytosis and phosphatidylinositol 4-kinase III alpha in the replication of the hepatitis C virus .