For the analysis of the cocaine, tramadol, and their metabolites the analytical column was an Acquity UPLC BEH-C18 column (3.0 x 100 mm, 1.7μm. Waters). The column temperature was set at 50°C. Mobile phase A was aqueous 0.1% ammonium hydroxide and mobile phase B methanol. The gradient started at 10% B and a linear profile curve was used for the change in mobile phase composition followed by re-equilibration time of 2 min (10% B). The total LC run time was 7 min. The flow rate was set at 0.6 ml/min and injection volume to 10 μL. Data were acquired and processed using LabSolutions (version 5.99 SP2, Shimadzu Corporation, Kyoto, Japan). Table summarizes the LC, MS optimized parameters for the analysis of eight drugs of abuse and five cannabinoids.