Thymoquinone analysis is to be done at a wavelength of 254nm. A clear peak of thymoquinone was obtained at a retention time of 29mins and the resultant extract was collected in a sealed wrapped contained and stored immediately at 4 0 Celsius. The resultant graph has been depicted in Fig . A standard graph for thymoquinone was used to compare the obtained peaks. On comparing it was observed that the retention time for thymoquinone is at 29mins, thus we can conclude the extraction of thymoquinone from HPLC, a detailed FTIR analysis has been performed to confirm the presence of thymoquinone.