Performance-wise, the efficiency of the af2rave protocol primarily depends on the efficiency of the MD simulations. Some programming is also needed to assemble the parts together. For proteins with 200 to 300 residues, the folding module requires approximately 3-10 seconds to generate one structure, depending on the MSA depth. The feature selection module does not involve computationally intensive steps. The protocol then requires short MD simulations from several cluster centers. These simulations require the most GPU hours but are highly parallelizable, depending on available resources, and typically take from a few hours to a day. Afterward, the timeseries data are processed using the AMINO algorithm.