Overall, this work investigates the deliverability and cation-transport behaviour of SSAs 1-3. They posed a challenge to study due to their fluorescent nature, propensity to aggregate, and the need for a TBA counter ion to modulate activity. We therefore used a variety of techniques such as the fluorescent HPTS (8-hydroxypyrene-1,3,6-trisulfonic acid trisodium salt) assay, ion-selective electrode studies with both potassium and chloride selective electrodes, carefully designed novel co-transport assays and patch-clamping. Furthermore, to gain a better understanding of the deliverability of the active ionophore, the EC50 (the effective ionophore concentration required to mediate 50% ion efflux after 330 s) as a measure of transport activity was compared in a range of delivery vehicles: DMSO, 95:5% water:ethanol and 100% water.