parameters, distance values are the most sensitive to trns/rep. With the same amount of enhancement in any one of the parameters, trns/rep leads to the highest reduction in distances. Most striking from Figure is the fact that there may be cases where distances do not diminish to zero even on increasing trns/rep, ensemble size and transition length to 100, 100 and 2 ns respectively. This is a major limitation of the NEQ approach, not to mention the high computational costs associated with such parameter adjustments.