Analytical scale biotransformations were performed using the specified amine (5 mM) and the relevant enzyme (20 µM) in 100 mM KPi buffer pH 7 with 20% DMSO containing 1 mM pyridoxal phosphate and 200 mM pyruvate. The reaction mixture was incubated for up to 24 h with shaking at 800 r.p.m. The reaction was quenched with 1 volume acetonitrile with precipitated protein removed by centrifugation (14,000g for 10 minutes) with the supernatant used for UPLC analysis. For chiral analysis, reactions were quenched by the addition of 100 μL of 5 M NaOH and extracted with EtOAc (250 μL x 3). The organic phase was dried over MgSO4 and analysed by normal-phase HPLC.