In this study, we used BODIPY functionalized silicon oxide microchips as a device for intracellular GSH sensing (Figure ). First, two types of BODIPY-based probes as the GSH sensors, called Bdpy1 and the reversible probe Bdpy2, were synthesized and immobilized onto the surface of cuboid SOµC previously microfabricated using the photolithographic techniques. The capability of the prepared SOµC-Bdpy1 and SOµC-Bdpy2 for long-term tracking of GSH was evaluated first in GSH aqueous solution during different time intervals and at various intracellular range of GSH levels. Then, the sensitivity of functionalized microchips was investigated through in vitro experiments in HeLa cells for the quantification and long-term tracking of intracellular GSH.