Kirchmajer et al. synthesized and characterized genipin crosslinked gelatin hydrogels and found that they could be used as vascularisable soft tissue scaffolds having pro-angiogenic effects when used in combination with PMA (phorbol-12-myristate-13-acetate), a stimulator of angiogenesis. Gattazzo et al. found that gelatin-genipin hydrogel could be used in skeletal muscle tissue engineering. Gelatin-genipin hydrogels have also been used in combination with other materials to generate composite hydrogels. It is the case in Xiao et al. where gelatin, known for its bioactivity, was combined with silk fibroin which has higher mechanical integrity and slower degradation. This composite was shown to be a potential candidate for cartilage, non-load-bearing bone tissue engineering and regeneration. Cui et al. mixed gelatin with chitosan before crosslinking to obtain interpenetrating polymer networks. It was found that this hydrogel was pH-sensitive and could then be used in drug delivery or sorbent applications. Wound dressing and healing applications have also been investigated. For instance, Ilkar Erdagi et al. synthesized genipin-crosslinked gelatin interpenetrated diosgenin-modified nanocellulose hydrogels and loaded neomycin drug. The hydrogel then showed good antibacterial activity against Gram-positive and Gram-negative bacteria suggesting it might have utility in wound dressing applications. Other gelatin-genipin hydrogel systems have been loaded with drugs and exhibited anti-bacterial activity. Here we show that genipin-crosslinked gelatin hydrogels can be used to detect the presence of Pseudomonas strains releasing active metalloproteases. Addition of a therapeutic layer also demonstrated the potential use of this hydrogel for the treatment of these bacteria.