The herb Centella asiatica (L.) Urban (Rau má) belongs to the plant family Apiaceae. It is a wellknown and widespread herb, especially in south and south-east Asian countries such as India, China, Malaysia, Australia, Madagascar, Vietnam, and Sri Lanka, and further afield, for example in South Africa. It is used as food and in traditional medicines. In Vietnam, a drink made from the leaves of Centella asiatica is very popular, in part for its refreshing properties in the summer when the temperature is very high , but also as a liver tonic and for liver detoxification. The leaves are also used as nutritional vegetables . C. asiatica contains flavonoids: kaempferol, quercetin and their glucosides, which are the anti-oxidants. It also contains other nutrients such as the glycerides of the fatty acids like oleic acid, linoleic acid, linolenic acid, stearic acid, palmitic acid and elaidic acid, as well as amino acids, carotenoids, and vitamin C. The Vietnamese use C. asiatica for the treatment of a number of conditions including cirrhosis, icterus, skin diseases, tuberculosis, leprosy, dysentery and cholera. Other cultures use the herb medicinally in diverse ways. In China C. asiatica has been used for treatment of headache, toothache, the common cold, and viral hepatitis ; Tan S. Ch. et al., 2021), while Indian traditional medicine uses C. asiatica as diuretics and tonics . These traditional uses have prompted scientific investigation of C. asiatica, and it has been shown to exhibit antimicrobial , anti-inflammatory , anti-oxidant , neuroprotective , wound-healing , anti-depression , and even memory enhancement properties. The most important biologically active constituents of C. asiatica have been identified as the triterpenoids asiatic acid (AA), madecassic acid (MA), terminolic acid (TA) (Fig. ) and their glycosides . Trends in potential applications of Centella asiatica phytochemicals in the food industry and medicine have recently been reviewed . Biosynthesis is far more economical than total synthesis for the production of the pharmaceutical products from C. asiatica triterpenoids, so is important to be able to control the accumulation of these triterpenoids during cultivation of the herb. Furthermore, knowledge of the occurrence of the main bioactive triterpenoids as their aglycones or as their glycosides is important before harvest and processing of the herb. It is known that different C. asiatica accessions have different yields and ratios of triterpenoids, 1-3 with "elite" lines yielding triterpenoid content above 4% of the dry weight. 3 Unshaded fields have been shown to give higher levels of these compounds in comparison to 50% shade, 4 with a second study validating the benefits of higher intensity light. 5 The colour of light used has also been assessed, though this may not be a variable open to many farmers. 6 Seasonal factors have been assessed, with one study reporting that hot and humid conditions are optimal for triterpenoid production 7 and another indicating that 25 °C was optimal, while bioactive compound content decreased significantly at 15 °C. 8 The biosynthetic pathways for these triterpenoids are known, and regulation of them has been tried in plant culture. 9 To the best of our knowledge, there are no studies which have compared agrochemical and organic growth methods.