Cluster sub-profile analysis of the CPA data recorded for the active Enamine revealed that at 30 µM, 106 out of 143 active compounds could not be assigned to any cluster (74%; 28 out of 44 active compounds at 10 µM (64%)). This finding suggests bioactivity that differs from the reference compounds employed for the cluster assignment and it was confirmed by means of UMAP analysis with the full morphological CPA profiles of these unassigned compounds.