Figure Combinatorial glycan composition assignment is aided by including all observed charge states, adducts, and MS misassignments using Dataset 3 as an example. A Utilisation of all expected adducts improves accuracy of glycan composition assignment. B The off-by-one error caused by MS misassignment of the monoisotopic peak can impact accurate-mass precursor searching. C MS2 scan assignment rate across GlyCombo features including multiply charged adducts, inclusion of more than one adduct type, and allowing for off-by-one monoisotopic mass assignment errors.