The antibacterial activity of the extracts of R. cordifolia and isolated compound was examined at the concentration of (100, 75, and 50) µg/ml against four pathogenic bacterial strains: two Gram positive S. aureus, S. pyogenes and two Gram negative E. coli, P. auruginosa (Table ). The result revealed that chloroform/methanol (1:1), and methanol (100%) crude extract showed the zone of inhibition ranging from 8 to 23. The chloroform/ methanol (1:1), methanol extracts, and isolated compound were active against all tested pathogenic bacterial strains (S. aureusa, S. pyogenes, P. auruginosa, and E. coli) at concentration of (100, 75, 50) µg/ml. As described in the literature (57,58) R. cordifolia is traditionally used to treat skin tissues damaged by injury or infection, abdominal complains, hemorrhagic, wound caused by bacteria. Hence, based on the relationship between traditional use and result of biological activities of R.cordifolia, one can decided that root extracts of this plant may be recommended as a remedy against bacteria caused skin infection, abdominal complains, hemorrhagic and wound. Photo of zone of inhibition measurement results are depicted below in Figure .