To demonstrate this approach experimentally, we have acquired 1 H, C methyl-SOFAST-HMQC spectra of uniformly C-labelled ubiquitin (277 K, 600 MHz). A sampling schedule was prepared based on a 35 Hz 1 J CC coupling constant, with a 40 ms acquisition time (Fig. ). 7 complex points were omitted either side of the zero crossing at 14.3 ms, resulting in 12% sparsity and a mean sampling density n = 2.8. The number of skipped points was chosen empirically: when too many points were omitted the quality of NUS reconstruction was reduced, while when fewer points were omitted small differences in intensity due to variations in J couplings were amplified by the large number of scans required near the zero crossing, again resulting in artefacts. In practical terms, an increased number of skipped points also resulted in a reduced acquisition time (lower n). Uniformly sampled and NUWS experiments were acquired using the sampling, apodization and NUS scheme in Fig. , and representative FIDs are shown in Fig. . The phase inversion following the zero crossing can be clearly observed in the NUWS signal, resulting from application of a negative apodization function (Fig. ). NUS reconstruction was performed using SMILE (Fig. , red) and the final 2D spectra are plotted in Fig. , together with a constant-time HSQC for reference (constant-time period 2T = 53.2 ms).