The addition of ZrO 2 -coated beads post deuterium labeling offers a sophisticated strategy for depriving the protein samples of lipid components under HDX quench conditions . Yet, beads need to be removed through filtration before sample injection into the mass spectrometer, which is laborious and time consuming, thus, may affect reproducibility among technical replicates and inevitably increases the D-to-H back-exchange . This process has recently been automated for HDX-MS applications: Anderson et al developed a robot-assisted workflow with nanofilter vials, where the labeled protein is transferred to the base of a filter system containing ZrO 2 beads, and after binding of phospholipids, the sample is filtered through a nanofilter cartridge by a LEAP X-Press module harvesting the protein for subsequent injection.