The full EnzymeMap database is freely available via Zenodo at doi.org/10.5281/zenodo.7841780 as a CSV file, with columns as displayed in Table . The benchmarks described in this study use version 2.0 of EnzymeMap. The columns natural, organism, protein_refs and protein_db contain information as obtained from BRENDA without any curation or verification. We note that many reactions occur both as natural and non-natural reactions, often in different organisms, but sometimes also in the same organism. Since this information together with the original text of the reaction can be used to link an entry back to BRENDA, we chose to keep this information. Depending on the intended usage of the database, we recommend to first filter for the reactions of interest, and then remove duplicates stemming from information not needed for a specific application.