We analyzed for active site and interaction between proteinligand, after 20ns Molecular Dynamic simulation. Using Ligplot+ , we found that active site is at ASN138 (Fig. ). We analyzed further for Hydrogen Bond interactions between Protein and Ligand, Results are summarized in Table . Amino acids LYS47, ASN138, ASN781, THR141, THR710, SER709, SER778, SER784, TYR129 which are forming Hydrogen bonds with Ligand. We analyzed further, for conformation changes during simulation on above amino acids at active sites. From these amino acids, we observed, ASN138 and SER709 only changed during this simulation. This indicates ASN138, SER709 will actively play and change in conformation during Protein-ligand bond interactions. protein RNA-dependent RNA polymerase CHEMBL3120791 which is in clinical trial for HCV can be potentially used for SARS Coronavirus 2. This work can be extended for Free Energy Perturbations (FEP) with 100ns and above for further analysis.