Moreover, the results in show a lateral flow assay where cortisol and the aptamer were allowed to incubate and react for 15 min. at 37°C yielding a sensitivity of 0.37 ng/mL (1.0 nM). However, for their study it is unknown whether the binding of hydrocortisone to the cortisol aptamer required a longer reaction time. Potentially, the sensitivity of the assay could have been increased by increasing the incubation time. To avoid dilution of the saliva sample in the TO-aptamer solution, a coated ELISA plate can be used. Contrary to the chosen procedure in the present study, the concentration of the TO-aptamer complex remains constant throughout the analysis preventing dilution of the TO-aptamer solution. Even though the 0.5 µM dilution of the TO-aptamer solution is small in this study, it will most likely have an effect on the final concentration determination as fluorescence is a very sensitive analysis method.