First, endpoint analyses were conducted within 1 minute of assembling all components in the 96well plate. Next, absorbance spectra scanning took place after a 5-minute interval. For the limit of detection assessment, the 0.5x serial diluted concentration was employed to provide accurate detectable range determination monitored by absorbance endpoint scanning mode for up to 3 minutes following the addition of all chemicals to the 96-well plate. Each measurement was replicated at least three times. LOD within analytic perspectives can be calculated via the formula: LOD concentration = 3 * S/ M, S of the standard deviation of blank signal, and M as a slope of the calibration. Practical LOD can be obtained via a distinguishable limit point due to the colorimetric assay, which is generally above the calculated LOD value.