The last snapshot of the 20 µs-long trajectory of the smaller plasma membrane patch simulated from an earlier study was converted to an all-atomistic representation using ezAlign with its default options. The resulting atomistic systems were composed of ~27.9K particles. Using these initial coordinates, three independent simulation systems were built, energy-minimized for 500 steps, and equilibrated following the six-step equilibration protocol suggested by CHARMM GUI's membrane builder with the modification that the number of steps in each step was doubled.