Analytes were evaluated for purity and size using SDS-PAGE. Concentration was measured for all proteins using BCA assay (Thermo Pierce cat. 23225). Samples were premixed 1× NuPAGE LDS Sample Buffer (4×, Thermo Pierce cat. NP0007) and heated at 70 o C for 10 minutes. Gels with a 4-12% Bis-Tris gradient were used to achieve separation. Coomassie Imperial Protein Stain (Thermo Pierce cat. 24615) was used to visualize bands. Novex Sharp Pre-stained protein standard (Thermo Fisher scientific) was used as a molecular weight marker.