In terms of coverage, a significantly richer lipid map featuring 1.7-fold more total annotations and 2.1-fold more lipids with CVQC < 10% was produced by μF/mnESI setup from 15x less sample amount. Our observations were in line with the results reported for nLC/NSI measurements of yeast cultures 7 and human plasma 9 in terms of expanded lipid coverage and increased analytical sensitivity. The gain in annotation rates was, however, smaller than for nLC/NSI. It may be first attributed to decreased ion sampling at the MS inlet due to the placement of M3 emitter further away from the transfer capillary compared to nanoflow setups. The increased voltage requirements of the silicon chip and the higher conductivity of the buffered mobile phases typical for lipidomics made the mnESI setup indeed more susceptible to discharge, as observed during method development. The difference in annotation rates may be also due to the transfer tube diameter in higher end MS models (e.g. Thermo Fusion Lumos) enabling better ion sampling . Finally, the slower collection of MS 2 scans in our Top4-capable instrument, compared to more performant orbitraps with Top10 to Top40 options, might have played an important role. Further increase in annotation rates on our setup could however be achieved with iterative strategies .