One such method to assess both structural and dynamic allostery from MD simulations is the CARDS (Correlation of all Rotameric and Dynamical States) methodology. CARDS is based on our observation that a single degree of freedom (e.g. a dihedral angle) can transition between "ordered" regimes, wherein it undergoes small fluctuations within a single structural state, and "disordered" regimes wherein it undergoes bursts of transitions between different structural states (Fig. ). Similar "dynamic heterogeneity" has been observed in the physics of glasses, where it has been shown that a single degree of freedom's local environment can either facilitate dynamics by flattening out the effective free energy surface that degree of freedom experiences or freeze out dynamics . CARDS identifies ordered and disordered regimes based on two kinetic signatures: the average time a degree of freedom persists within a structural state and the typical timescale for transitions between structural states. For many dihedrals, we observe that the typical time that elapses between structural transitions, which is dominated by segments of a trajectory in disordered regimes, is orders of magnitude smaller than the typical persistence time in a state. Based on these kinetic signatures, CARDS assigns segments of trajectories to dynamical states (i.e. ordered and disordered regimes). CARDS then quantifies correlations between the Chapter structural and dynamical states of different dihedrals.