After MMP-7 incubation, the corresponding autocorrelation curves shifted to faster diffusion times indicating cleavage of the peptide bonds (Figure ). In the presence of MMP-7, a twocomponent fit of the autocorrelation curves revealed complete disappearance of any structures with original nanogel sizes. We only detected the presence of free Cy5-polymer chains (54 % of total diffusing species) and some bigger, possibly entangled degradation products (DH value of 35 ± 6 nm, 46%, shown in Figure ), demonstrating MMP-7-dependent peptide cleavage and complete nanogel disassembly below the LCST. By comparing the nanogel signal intensity (counts per particle, CPP in kHz) to the dye intensity, the number of dye molecules per single nanogel could be determined. Upon enzyme-mediated nanogel degradation, the calculated number of dyes per particle changed from about 54 dyes per particle before degradation, to about 2 dyes per particle for the degradation products. This is a further indication of nanogel disassembly. Together with the DLS analysis (Figure ,b), enzyme-responsiveness of our nanogel system was successfully demonstrated.