In the NanoBRET-based ternary complex formation assay, SD75, although a less potent degrader than XL01134, induced higher ternary complex than XL01134. However, it should be noted that this assay was carried out in the permeabilized HEK293 cells, and SD75 is likely to induce less intracellular ternary complex formation given its relatively lower permeability comparing to XL01134 (Figure ). The relative permeability (intracellular availability) of each compound was obtained by querying VHL engagement or LRRK2 engagement under live-cell and permeabilized-cell conditions (Figure ).