Pesticide analysis was conducted on 21 white and 16 red wines (Table ). Wine samples required online dilution for efficient trapping of analytes on the C18-AQ trap column. 27 pesticides were detected (Figure ), and the concentrations were estimated using single-point calibration (Table ). About 70 % of the pesticides found were fungicides, and 30% were insecticides, including neonicotinoids like acetamiprid and imidacloprid. In many cases for wines, scheduled MRM HR experiments were necessary to confirm identification, as the quality of the SWATH acquisition spectrum, even with Zeno pulsing, was limited. MRM HR with CID, as well as EAD further confirms that the analyte detected was the correct one. Representatives MS/MS spectra for the detected pesticides in wines can be found in Figure to S41.