Compared with vacuum-based techniques, ambient SCMS methods offer greater flexibility, enabling in situ analysis of cells within their native or near-native environments. This capability makes ambient SCMS more suitable for live cell studies. However, the sensitivity of ambient techniques is typically lower than that of vacuum-based methods, due to ionization efficiency caused by interference from matrix molecules . Additionally, the throughput of most ambientbased methods tends to be lower, limiting their applicability for studies requiring a large number of cells. Despite these challenges, significant advancements have been made to improve the throughput of ambient SCMS techniques, making them a valuable tool for minimally invasive live-cell analysis . Many ambient SCMS techniques typically employ physical probes, lasers, or charged solvent droplets to facilitate analyte sampling and ionization.