The dietary requirements of insects and humans for essential amino acids are similar, highlighting the nutritional significance of amino acid composition in arthropod hemolymph . Given the potential applications of hemolymph in human and animal nutrition, as well as in medical and biotechnological research, this study aims to analyze the amino acid profile of hemolymph from various arthropod species. By identifying the composition and concentration of amino acids in different arthropods, this study provides insight into their nutritional potential and potential applications in culture media for microbial propagation.