Clofazimine (CFZ) quantification was performed by UV-Vis absorption on a BioTek Microplate spectrophotometer, Thermo Fischer Scientific (MA, USA) using a calibration curve with the known amounts of CFZ (Figure ). Samples were prepared Rotilabo F-Type 96 well plates, Carl Roth GmbH and Co. KG (Karlsruhe, Germany) with the constant volume of 200 µl. Spectra were recorded from 300 to 600 nm at 298 k. CFZ absorption was detected at 450 nm. Prior to UV-Vis absorption measurements, the aqueous formulations were diluted with ethanol.