State-of-the-art all-atom force fields are used for Molecular dynamics (MD) simulations of protein-lipid systems: Amber ff19sb for polypeptide chains, lipid21 for lipids, four-point OPC water model , and ionic models optimized for OPC water model with Amber20 and AmberTools21 software . Ccptraj, which is a crucial part of AmberTools package, was used to perform most of the analysis, such as reimaging, root-mean-squaredeviation (RMSD), root-mean-square-fluctuations (RMSF), radius-of-gyration (Rg), maximum distance from the center of mass to the any heavy atom of the protein (Rg-max), phi and psi dihedral angles, number of contacts between lipid-heads and amino-acid (contact was present if the αC atom of the amino-acid residue was not more than 7.0Å from the phosphorus atom in the lipid head) secondary structure content with DSSP algorithm , angles between helices and helix and Z-axis of the periodic box and solvent accessible surface area (SASA). The distance of αC atoms from the lipid bilayer center in the Z-axis was calculated using in-house script.