After completion, experimental data were analyzed utilizing FortèBio data analysis9 software, reference data (protein, inhibitor and buffer) was subtracted from the raw data and according to baseline aligned. Global fitting of 1:1 kinetic model was applied to determine binding kinetics and statistical errors. The same experiment repeated for three mutant-versions of PLY. Later, raw data of association-dissociation steps of each experiment, at 4 µM of PB-2, were highlighted and transferred from FortèBio data analysis9 software to Graphpad Prism 6. Finally, data of mutant versions and PLY-WT were combined in a graph and protein-interaction with PB-2 (4 µM) was highlighted as association and dissociation steps.