Primarily, gold nanoparticles were functionalised by linking thiolated nucleic acid to gold nanoparticle surfaces and aggregated through hybridisation of a sensory aptamer strand. The sensory aptamer strand was hybridised by two different complementary nucleic acid strands, such that SH-Sequence-A and SH-Sequence-B-Biotin were complementary to the 3' and 5' of the aptamer, respectively. To prepare gold-aptamer-nanoconstructs that utilise the signal transduction mechanism of a lateral flow assay a mixture of SH-Sequence-A and SH-Sequence-B-Biotin were used in a 1:1 ratio. Biotin functionalised nucleic acid gold constructs bind membranes doped with streptavidin at predefined locations.