The data have been analyzed with Dfind software (Biolin scientific) by using the Broadfit model, to fit the data and extract the mass adsorption values.. Dermal fibroblast culture and seeding. Human dermal fibroblast cells (HCA2; hTERT)immortalized human dermal fibroblast cell line ) were cultured from passage 22 in T75 flasks with Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% 2 mM L-glutamine and 1% penicillin-streptomycin (PS) in an incubator (37°C and 5% CO2). The medium was aspirated and replaced every 2-3 days. When 70-90% confluency was reached, fibroblasts were harvested with trypsin (0.25%), and Versene TM solutions (EDTA Na4, 0.2 g/L) in phosphate buffered saline in a ratio of 1/9, centrifuged, counted, and resuspended in DMEM in a T75 flask at the desired density. For cell seeding onto substrates, fully confluent fibroblast cells (HCA2) cultured at 37 o C at 5% CO2 were seeded at a density of 60,000 cells per well (24 well plates). Cells were left to adhere for 24 h in the incubator and resulting cell cultures were examined via bright field microscopy on days 1, 3, 5, and 7. The culture medium was exchanged every other day, using ascorbic acid (L-ascorbic acid-2 phosphate; 50 µg/mL) supplemented DMEM.