In parallel to the zebrafish embryo bioassays, we prepared methanolic extracts of the consumergrade PCL to screen the bioactivity and composition of plastic-associated chemicals using highthroughput in vitro bioassays and non-targeted GC×GC analyses, respectively. Methanolic extracts from consumer plastics have proved instructive for assessing the toxic potential of leachable plastic-associated chemicals. Additionally, analyses of solvent extractable material are routine parts of food-contact and medical device regulatory frameworks. Blank-corrected methanolic extractable mass for the consumer-grade PCL was 6.59  4.16 mg/g PCL (n=3). The variability in extractable content (coefficient of variation = ~63%) provides a possible explanation for some of the variability observed in the zebrafish bioassays. Due to pellet variability, replicates with lower mortality could have been exposed to pellets with less leachable content and viceversa.