Generally, researchers have focused on the difference between droplet and non-droplet phases but have overlooked the difference in the droplet characteristics depending on the component ratio. Our results suggest that the characteristics of the droplets formed via LLPS vary with the conditions. This data showed that droplets formed in a cell have various characteristics depending on the local concentration of macromolecules and droplets even from the same components could behave various way to outward stimulus like stress and/or accumulation of metabolites Careful obtaining of phase diagram of LLPS would provide such information Next, according to the phase diagram, we established an experimental system to observe the nonequilibrium process of droplet formation and disappearance, which occurs in real cells (Figure , S1, Supplemental movies 1 and 2). We used an ATP and PLL concentration gradient in a chamber to mimic all phase boundaries and inner and outer areas of the twophase diagram shown in Figure to model the nonequilibrium process of the droplet life (Figure ).