The diffusion coefficients were obtained by fitting a rectangular FRAP pure diffusion implemented into the software. To track the EV and SiNP motion time series measurement was carried out using the previously mentioned ZEISS microscope with a 20X magnification objective. In each measurement, 50 images were taken. To acquire the particle tracking data ImageJ was used. The first brightness and contrast adjustment and background subtraction were applied by setting the rolling ball radius to 2 pixels. Afterward, particle tracking was carried out by using MTrackJ plugins. The snap feature was set to maximum intensity and a 5x5 pixel snap range was used. The maximum distance to the start point was exported and plotted as a histogram by using GraphPad Prism 8.