We assessed the impact of EBEC data collection on the overall quality of MicroED data collected with settings typically used for small molecule ab initio structure determination (Supplementary Figures ). To assess the quality of EBEC data with respect to conventional datasets, we directly compared diffraction from salen ligand crystals, measured using either the scintillator-based CMOS-based camera (CETA-D) or the Apollo detector. Diffraction movies were recorded with both the CETA-D and the Apollo from individual salen ligand crystals with an incident electron beam flux of 0.01 and 0.045 e -/Å 2 /s. EBEC data showed improved contrast and signal-to-noise (Supplementary Figures ), but, under these conditions, a large fraction of measured pixels in EBEC patterns had counts in the range of 10-80 e-/pix/s, where some CL might be expected (Supplementary Figure ).