The results presented above demonstrated that TBPBB effectively measured ROS level and their spatial distribution within germinated pea seeds. Experiments conducted in soil culture validated the usefulness of TBPBB in selecting pea seeds under carious stress conditions. Follow-up planting trials with two pea varieties reinforced these findings. This AIEgen based methodology was anticipated to be effective for screening various pea seed varieties. To validate this, other 10 pea varieties were cultivated in the growth chamber under both water and alkaline conditions (Table -S27, Fig. ). The typical photos and fluorescence intensity under two different growth trends in natural water and alkaline conditions were shown in Fig. .