Single objective optimization of both t 2 and VAMP2 eq (2) increased t 2 for Chignolin and BBA. For Chignolin the increase was modest, between 2.4 %-5.5 % for all four objective functions. The single objective optimization of t 2 had the smallest increase (Figure , panel (a) red squares) while the multi-objective optimization of the VAMP2 eq (2) and VAMP2 eq (2)/VAMP2 eq (3) gave the largest increase in t 2 (Figure , panel (c) blue squares), although the increase in the gap was modest (see Figure ).