Since it could be possible that removal of SP do not change the 24C conformer affinities, the effect of SP removal on the 24C affinites were also explored. For that, residues 1-27 (SP) were computationally deleted from CSP (CSP-SP) and the resulting ADV affinities compared to CSP+SP. The corresponding results predicted ~ 15-fold reduction of rank profiles for CSP-SP (Figure , red open small + large circles and red solid small + large circles).