Subsequently, when BDO monomer is consumed, DLD-DA sequences are formed in second order, and in the end, the copolyester microstructure is more blocky. On the other hand, when the synthesis is carried out in bulk, it restricts enzyme mobility, causing BDO-DA and DLD-DA sequences to be catalyzed at similar rates, resulting in the formation of copolyesters with a random microstructure. Similar results were provided by Ilarduya et al., where enzymatic polycondensation of copolyester materials in bulk led to products with a random microstructure.