For B100 fuel life cycle, total 23 parameters were identified and value of each was varied ±10%. It is observed that the parameters: electricity use, life cycle span, seed yield, methanol use, urea fertilizer use, NOx emission factor, and steam use are highly sensitive. While the parameters like diesel use in irrigation and cultivation, hexane use in solvent extraction, P2O5 and K2O fertilizer use in cultivation, NaOH used in transesterification, transport distance, CO emission factor, CO2 emission factor, PM emission factor, and hydrocarbon emission factor are significantly less sensitive to the life cycle environmental impacts of B100 (Table ).