We compared platelet parameters extracted from iSCAT imaging with those obtained from identical samples using AFM, TEM, and CLSM. Assembly of fluorescently-labeled platelets was initiated by adding 10 µL of 1 mM (10 mg/mL) aminochloromaleimide (ACM) -coupled homopolymer and block copolymer unimer mixtures (ACM-PCL 45 :ACM-PCL 45 -b-PDMA 348 , 1:1, w:w) into 1 mL 0.25 µM (10 µg/mL) PCL 45 -b-PDMA 348 seed solution in ethanol, followed by mixing via shaking. At predetermined time points, identical aliquots were taken, quenched by water addition, and analyzed using each technique. Further details for AFM TEM, and CLSM measurement are provided in the Experimental Methods section. Fig. shows that platelet morphology and area information extracted from AFM, TEM, CLSM, and iSCAT are comparable (Fig. ). Under these reaction conditions, platelet formation is rapid, with the assembly completed within two minutes of our first time point; essentially faster than the time resolution of AFM, TEM, and CLSM measurements.