The interaction of copper MOFs with phospholipids is not widely studied. Yet, Lee et al. reported a copper MOF immersed in phosphate-buffered saline formed a flower-like deposition on the surface of MOF due to the reaction of copper cations with phosphate anions. This copper-phosphate coating prevented the release of Cu 2+ ions to a great extent making these MOFs a stable candidate for aqueous physiological applications. Our results showed many GUV@MOFs in the imaging wells with both CuBDC and HKUST-1 when tested with each lipid mixture, suggesting the affinity of copper for the phosphates of lipid molecules (Figure , S4, S5). Supplementary videos SV5 and SV6 provides a visual representation of POPC:POPG:Chol GUV@MOF with CuBDC and HKUST-1. The mean vesicle diameter with CuBDC was within 10 µm range for all lipid mixtures and relatively close (18 -23 µm range) with HKUST-1 (Figure ). The POPC:Chol lipid mix yielded a vesicle size similar to POPC:POPG:Chol lipid mix while largest diameters observed in GUVs formed from DOPC:DPPC:Chol lipid mix (Figure ). The number of GUVs formed with negatively charged lipid composition in presence of CuBDC was significantly higher than all other MOFs with this lipid composition.