We therefore produced BRD4(1) L94C which was obtained in high yields and purified to homogeneity using immobilized metal affinity chromatography (IMAC) and size exclusion chromatography (SEC) (Figure ). Both BRD4(1) L94C and BRD4(1) WT had similar melting temperatures of 49.0 °C and 49.4 °C, respectively, determined using differential scanning fluorimetry (DSF), suggesting that protein stability had not been compromised by the mutation (Figure ). Furthermore, BRD4(1) L94C