To quantitatively describe the degree of heterogeneity, we suggest defining a relative peak amplitude below 0.32 as homogeneous, 0.32 -0.4 as partially heterogeneous, and the relative peak larger than 0.4 as heterogeneous. To further analyze the shape of the normalized histograms, we simulated 100 homogeneous droplets with a radius of 1000 nm and three different localization precisions σlp of 250 nm, 100 nm, and 40 nm. For this simulation, we set the localization accuracy σac to 30 nm to see the contribution of the σlp to the RPD histogram's first peak. For σlp of 250 nm, we obtained two broad peaks in the RPD histogram (Figure ). Decreasing σlp to 100 nm and 40 nm, respectively, led to an increasingly clear separation between the local maxima and the narrowing of both peak widths (Figure ). To validate the simulation results, we performed fitting on the data. The fitted values are normalized against the maximum distance and resulted in σlp values (LP) of 42 ± 3 nm, 103 ± 5 nm, and 257 ± 5 nm for the small, medium, and large σlp, respectively.