On the other hand, fragmentation may improve the sensitivity of particular analytes. For example, when 1,3,5-trinitroperhydro-1,3,5-triazine (RDX) was fragmented to ([M-NO2-HNO2] -, m/z 129), the limit of detection (LOD) was found to be 10.33±0.45 pg, whereas the LOD for its adduct ion, ([RDX+NO3] -, m/z 284) was 30.75±0.95 pg . According to , RDX's sensitivity has increased as it has fragmented to ([RDX-NO2] -, m/z 176) . The better sensitivity for RDX was also found as RDX fragmented to [RDX-NO2] -that appeared at m/z 176 under 0.8 Torr of air pressure using glow discharge ion source . They also reported that fragmentation of the parent ion, ([RDX-NO2] -, m/z 176), to ([CH2NCH2NNO2] -, m/z 102) in MS/MS mode can detect only 0.5 pg of RDX . The results revealed that under the negative mode of operation, some of the fragmented molecules have a stronger electron affinity than the parent molecules. The ESI, nano-ESI, PESI, DESI, MALDI etc systems serve as both an ion source and a desorption process for semi-volatile and nonvolatile compounds. The essential step in the MS analysis is gasification of the analytes of interest. We recently developed an ultrasonic cutter-based desorption method for gasification of highly nonvolatile compounds such as explosives, drugs of abuse, herbicides, steroids, ionic liquids, rhodamine B, sugars etc, which were ionized using a homemade He-DBDI and analyzed using an ion trap MS .