After collection, all samples were transferred to sterile test tubes and stored at -80 °C. The frozen Alpha and beta diversity matrixes were used to compare the microbial diversity in the samples. OTU count and the Shannon Index were used as the alpha diversity measures to see the diversity within a sample. Bray-Curtis dissimilarity was used as a beta diversity measure to compare the microbial diversity between the two samples. A value of 0 in the Bray-Curtis dissimilarity indicated complete microbial similarity, while a value of 1 indicated complete dissimilarity between the samples.