Rice plants were sowed in pots (27 m 2 area; 4.5 × 6 m) with soil samples of all three districts including Faisalabad (FSD), Gujranwala (GUJ), and Sheikhupura (SHK). Each district has 10 pots in number and each pot contains 6 kg soil. Plants were irrigated at different intervals of time in a week and placed in sunlight to grow better.