Peptides are an important class of biomolecules that have been rapidly developed as therapeutical agents that cover areas such as metabolic disorders, skin infections, and oncology. Among many challenges in developing acceptable commercial peptide drug product is to demonstrate acceptable chemical and physical stabilities. This is accomplished through systematic stability studies, in which peptides as active pharmaceutical ingredient (API) or in the formulation matrix are stored at certain conditions, their chemical and physical stabilities are monitored over time to understand the effect of environmental parameters such as temperature, moisture and light. There are reported computer programs such as ASAPprime 5 utilizing humidity corrected Arrhenius equation, which predicts the stability of APIs using storage conditions such as temperature and humidity. Potency, individual degradation product and total degradation product are key quality attributes of peptide drug products and studied in chemical stability. High performance liquid chromatography (HPLC) is one of the most common analytical techniques for measurement of potency and degradation product. Peptides, especially in solution during storage or manufacture, can undergo conformational change from α helical structure to β sheets, then aggregate or form fibrils. These pathways of physical instability are undesirable due to their potential risk of immunogenic responses and impacting bioavailability. Thus, aggregation is a key physical stability parameter of formulated peptide product and need to be evaluated. One method for measuring the aggregation of peptides is through the use of Thioflavin T (ThioT). The knowledge gained from stability studies will be used to guide the design of formulation, establishment of shelf life and determination of proper storage and packaging conditions. Depending on the stage of drug development, the duration of stability study ranges from a couple of months to several years, which requires tremendous number of resources, and the process is lengthy.