The three methods (2x LC-vDMS-SIM/MS and 1x LC-MRM/MS) were applied to the quantification of 13 drugs of abuse in urine samples were collected from subjects who tested positive for THC and/or cocaine during roadside drug testing. Samples were spiked with internal standards (Cocaine-D3, Benzoylecgonine-D3, Tradamol-D3 and THC-D3 at 400 ng/ml) and centrifuged. Ten urine samples were first analysed by 1 min LC-vDMS-SIM/MS method for quantification of eight drugs of abuse/metabolites and qualitative identification performed for five cannabinoids/metabolites. Next the second longer method of four minutes was performed to confirm and quantify the cannabinoids THC, CBD, and metabolites. Samples were re-analysed by the reference LC-MRM/MS method.