An illustration of the use of these methods is the work of Lopes and coworkers on halcinonide. As a topical glucocorticoid, halcinonide is commonly used to treat dermatoses because of its potent anti-inflammatory and antiproliferative effects. However, it is reported that this drug and other drugs of the same type can cause adverse side effects (such as epidermis, dermis, etc.). To minimize these dose-dependent toxic effects, Lopes and coworkers encapsulated halcinonide in lipid nanoparticles (e.g., polymeric lipid core nanoparticles, solid lipid nanoparticles). Then, the encapsulation efficiency of the drug delivery system was validated by HPLC with UV detection at 239 nm. Using this method, the researchers confirmed that the encapsulation efficiency of halcinonide in nanoparticles was nearly quantitative, with the drug permeation assay confirming a reservoir effect. In the context of halcinonide, this analytical test proves that the LPN formulation can significantly reduce side effects while maximizing the therapeutic effect of the drug carrier .