Liu et al. used MD simulations to study Daptomycin's interaction with DMPC/DMPG (a bacterial membrane mimic) and pure DMPC (a mammalian membrane mimic). They found that Daptomycin oligomers were more stable in DMPC/DMPG mixtures than in pure DMPC, consistent with Daptomycin's selective activity against bacterial membranes. The simulations also revealed the formation of a narrow, water-conducting channel within the Daptomycin tetramer, allowing ions to pass through and disrupt the membrane's potential in bacterial cells . This channel was stabilized by interactions between the carboxyl groups of Daptomycin and Ca 2+ ions, which shielded the hydrophilic moieties of the peptide from the membrane's hydrophobic core.