The binding to lipid II inhibits cell wall synthesis, and serves as "landing terrain" for the subsequent formation of pores in the bacterial membrane . This two-step mechanism of lantibiotics is confirmed by the induction of membrane depo-larization exerted by lantibiotics like Nisin . The dual mechanism enables Nisin to target a broad range of Gram-positive bacteria, although it is weakly active against Gram-negative species due to the impermeability of the outer membrane .