Concerning the APL measurements (Figure ), the values recovered compare well with those obtained with the CHARMM36 AA force field for both DPPC and POPC bilayers at 323 K 110 . A decrease of ~16 and ~15 Å 2 was observed in AA simulations upon the addition of 30% cholesterol to DPPC and DOPC membranes, respectively 110 . This compares well with the ~14 and ~12 Å 2 decrease observed with our Martini 3 model. Martini 2 shows a slightly stronger condensation effect in unsaturated membranes, yielding a ~14 Å 2 decrease in APL for both DPPC and POPC membranes. This small difference is likely due to the increase in the center plane cholesterol population in Martini 3, which will be decreased when coupled with future lipid models. Still, these results show similar behavior from all three models.