of the types caused by injury or specific diseases, wounds should be covered by an effective dressing material, with features such as high biocompatibility, ability to retrain moisture, and mechanical properties well-suited to injured tissue and antibacterial action. Conventional wound dressings, such as gauze, must be changed frequently, and the risks of infection, secondary damage during removal, and their lack of biological properties for wound healing hampers their usage. Hydrogels have gained high interest for wound dressing and healing applications due to several outstanding properties, such as wound adhesion, moisture retention, skin like softness, resemblance to biological tissues, and biocompatibility, all of which provide favorable conditions for wound healing. Additionally, the three-dimensional porous structure of hydrogels provides a suitable scaffold for loading antibacterial drugs, which can then be released and delivered in a controlled process. The drug release is achieved through either diffusion or the passive degradation of hydrogel. Eventually, such degradation enables the dressing material to be removed from the wound without secondary damage. Degradable hydrogels are typically prepared by introducing a stimuli-responsive moiety into the backbone of a gel macromonomer, allowing degradation in response to stimuli such as pH, temperature, enzyme, redox state, etc. This stimuli-responsive hydrogel degradation serves in turn as a stimulus to release therapeutic agents in a controlled manner, or to ensure the hydrogel's ultimate removal in cases when it is used as a bandage for infected wounds. Among different approaches for "on-demand" degradable hydrogels, the redox-responsive approach has directed much interest toward polymer-based hydrogels, which are typically synthesized by introducing a disulfide bond in a polymer backbone. Disulfide bonds can be cleaved to two thiol groups in response to variation in environmental redox state. In particular, redox-responsive hydrogels show enhanced degradation in response to the overexpression of glutathione (GSH) or reactive oxygen species (ROS) in tumor tissues, wounds, or bacterial infection sites. The redox imbalance that prevails in the infected wound environment can stimulate either therapeutic agent release or hydrogel degradation. Recently, multiple redox-responsive hydrogels have been reported for improving wound healing, with applications including the release of therapeutic agents, mainly proteins. GSH is a tripeptide (γ-Glu-Cys-Gly), sulfhydrylcontaining compound that is abundant in different types of organisms; in fact, it is the most dominant intracellular thiol-containing tripeptide. It is a biochemical workhorse: it participates in the regulation of gene expression, cell proliferation and apoptosis, signal transduction, enzyme and protein functions, and oxidative stress in biological systems. Szabó et al. showed clear differences between molecular patterns of wound fluids in acute and chronic wounds through redox profiling. The study has shown that, similarly to radical scavenging activity, GSH levels were elevated in chronic wound fluids in comparison to acute wounds. In another study, Shukla and Rasik showed that in the analysis of 7 day wound tissue, higher levels of GSH were shown in athymic mice in comparison to the wound tissue of normal mice. In this work, we present a one-step straightforward synthesis of a disulfide bondcontaining hyperbranched polyglycerol macromonomer (SS-hPG). The synthesis is catalystfree and solvent-free, and we carried it out on a 30 g scale. The synthesized SS-hPG is employed as a building block of a redox-responsive hydrogel that is chemically crosslinked to 4-arm-PEG-SH via thiol-Michael addition click reaction. An antibacterial peptide, vancomycin, was loaded in the redox-responsive hydrogel to be released either gradually or on demand. Crucially, the viscoelastic properties, mechanical strength, and degradation of SS-hPG, and the release time of the loaded antibiotic, can be tuned by varying the concentration of the polymeric components in the hydrogel.