Once the high content of lipids in the cul vated microalgae was demonstrated and analyzed, a protocol for their extrac on was op mized. In this work we target the extrac on of preferably triglycerides, which can further be func onalized with photopolymerizable groups. Importantly, the extrac on method as well as the solvent choice (i.e. solvent polarity) are key to selec vely extract the lipids from microalgae-based biomass. While polar solvent mixtures, such as a 2:1 chloroform/methanol mixture, offer poor selec vity, non-polar solvents such as hexane are more suitable for the selec ve extrac on of triglycerides. Furthermore, cell wall disrup on is needed in most cases to extract lipids effec vely. For this purpose, sonica on and a high-performance dispersing instrument were employed. The presence of triglycerides in the extracts was confirmed using 1 H-NMR spectroscopy and thin layer chromatography (TLC) (Figure , Figure and Figure ). The O. aurita-based extracts contain triglycerides with 3.3 double bonds on average, while the double bond content is higher for the T. striata-based ones, with approximately 4.6 double bonds per molecule. These results are in accordance with the lipid profiles presented previously. It should be noted that the extracted triglycerides exhibit a dark green color due to the chlorophyll deriva ves from the microalgae.