where class membership is supplied in a discriminator matrix (Y-matrix) to decompose the systematic variation in the dataset (X-matrix) into Y-correlated (predictive) and Y-uncorrelated (orthogonal) variation . The separation between the predictive and orthogonal components facilitates the interpretation of the predictive loading vector and provides a direct measure of the influence each of the variables has in the model. The number of relevant components for the models was estimated through seven-block cross-validation (CV). Orthogonal components based on CV were reduced to further prevent overfitting of the OPLS-DA models.