All enzymes were synthesized and cloned into pCK900 vector and transformed into W3110 E. coli electro-competent cell by electroporation and plated on LB agar with 30 µg mL -1 chloramphenicol. A single bacterial colony was picked and grown in LB media (Teknova) supplemented with 1 % glucose and 30 µg.mL -1 chloramphenicol at 37°C, 200 rpm, 85 % humidity overnight. Following overnight growth, the culture was used to inoculate a shake flask containing TB media (Teknova) supplemented with 30 µg.mL -1 chloramphenicol and grown at 30°C, 200 rpm, 85 % humidity until the optical density (OD600) of the culture reached 0.7. Enzyme expression was induced by the addition of IPTG to a final concentration of 1 mM and the cultures further grown at 30°C, 200 rpm, 85 % humidity for 16 h. Cells were harvested by centrifugation at 4000 g at 4°C for 15 min and the media discarded. Cell pellets were frozen at -80°C prior to lysis.