This paper marks a critical advancement in the understanding of paclitaxel biosynthesis, providing a robust platform for further discovery and optimization. The successful reduction of unwanted byproducts through the modulation of T5αH expression, combined with the structural characterization of novel intermediates, opens up new possibilities for producing paclitaxel and related compounds in microbial or plant-based systems. shows Prompt B1, which provided emerging information regarding paclitaxel retrobiosynthesis by utilizing the 10 Paper Summary, while Prompt B2 provided a historical perspective regarding paclitaxel retrobiosynthesis by relying on models' trained parameters in this area of study. Using a similar approach, Prompt B3 provided information regarding an emerging perspective to engineering microorganisms for large scale paclitaxel production, while Prompt B4 provided the historical perspective in this research area. All 24 generations and 24 judged generations are available in supplementary S4 and S5 accompanying the manuscript.