For acquiring SMLM data, we used the same modalities reported previously . In short, the laser beam entered the excitation path of the microscope via a fiber-coupled laser engine (Omicron, Germany). After the fiber, the laser beam was collimated using a 60 mm achromatic lens (AC254-060-A-ML, Thorlabs) and reflected by a kinematic mirror (BBE1-E02, Thorlabs) into a top hat beam shaper (Asphericon GmbH). Then the beam was focused by a 150 mm lens (AC508-150-A-ML, Thorlabs) into the back focal plane of a 100x oil immersion objective (NA = 1.45, Nikon) through a polychroic mirror (ZT405/488/561/640rpcv2, Chroma). The emitted signal from a sample is collected with the same objective and passes an emission filter (ZET405/488/561/640m-TRF, Chroma). The signal was further reflected by a kinematic mirror and focused through a tube lens (MXA20696, Nikon) into a focal plane of the first lens (AC508-100-A-ML, Thorlabs) of a 4f system. Then the emitted light was reflected by another mirror before arriving at a deformable mirror (DMP40/M -P01, Thorlabs) positioned in the Fourier plane of the 4f imaging system. The light was then focused via a second lens (AC508-100-A-ML, Thorlabs) on a sCMOS camera (Prime 95B, Photometerics) with an effective pixel size of 112 nm.