For compounds constituting family 5, toxicity differences were observed between click compounds 34-36 obtained from the diruthenium azide derivative 9. Compound 35 showed important HFF toxicity at 1 µM, while derivative 36 was highly active against the parasite at 0.1 µM while remaining non-toxic to the host cells even at 1 µM. Interestingly, phenyl derivative 37 presented a similar toxicity profile to its analogue 34 with a different mode of connection to the diruthenium moiety. For compounds presenting the methylene-hydroxy group as substituent, 38 exhibited slightly increased HFF toxicity and a reduced antiparasitic efficacy when applied at 0.1 µM compared to 36. From family 5, compared to precursor diruthenium azides 9 and 10, only click products 36 and 39 exhibited an improved toxicity profile to the host cells, while still being active on the parasite.