Standardisation of the chromatographic methods employed among laboratories for LC-MSbased global profiling is a practical solution to this problem. While methodological and broader platform diversity is helpful for the field in that it promotes healthy evolution and supports independent validation of findings (e.g., by independent cohorts analysed by differing platforms arriving at the same results), tension exists between the desire to develop cuttingedge methodology and its undermining effect on the characterization and consolidation of knowledge from stable (standardized) assays. Across laboratories, siloed development of method-specific in-house libraries is pursued at great expense in parallel efforts to break a field-wide bottleneck. Despite efforts toward standardisation in the metabolomics field , greater incentives appear to be required for success in standardising data-generating methods. The open availability of such methods, disclosure of details and motivations driving key method-defining decisions, availability of quality documentation, demonstrated track record of performance and reproducibility, and accompanying open-source tools to facilitate interrogation of the resulting data are important factors for practitioners looking to adopt common methodology. However, gaining immediate knowledge of an extensive set of methodspecific metabolite annotations and the potential for further development of that knowledge as a part of a larger collective of cooperative laboratories is arguably the most attractive incentive to adopt and use established methodology. Such an approach has been previously implemented for the substantial advancement of gas chromatography mass spectrometry (GC-MS) establishing a precedent for successful standardisation. Sets of annotated metabolites are now being made publicly available for LC-MS methodology as well. Here we summarise a conscientiously developed and extensively used set of LC-MS methodologies for human biofluid analysis (urine and blood products), complete with the sample handling and preparation protocols required to make those biofluids amenable to LC-MS analysis, a description of reference materials used for quality control (QC) assessment, and the open-source software used to extract and prepare the data generated for subsequent analysis. Furthermore, the m/z and retention time measurements for more than 700 biologically relevant metabolites detectable in biofluids are provided, bringing immediate value to researchers wishing to adopt these approaches to reversed-phase chromatography (RPC) and hydrophilic interaction chromatography (HILIC) separation for LC-MS analysis. Together, these materials (summarised in Figure and Table ) constitute the National Phenome