The crystal structure file for S protein (PDBID: 6xlu) was downloaded from the PDB database. The domains such as Plasmodium antigen were then annotated. TryThrA_C A-C is located in the virus's extracellular region, as illustrated in Figure . TryThrA_C D is found both across the viral membrane and within the viral intracellular. The residue corresponding to the TryThrA_C A part is "ESEFRVYS" due to the truncation of 6xlu. "ESEFR" is the overlapping residue between TryThrA_C A and C1q A. 4.1m A overlaps residue "ESE" in the TryThrA_C A segment. The TryThrA_C A region aids the virus in locating and binding to the membrane of the red blood cell. S first binds to the complement receptor 1 on the red membrane before invading and anchoring the 4.1 protein. The TryThrA_C B-C region aids the virus in its continued invasion of red blood cells. The TryThrA_C D region assists S in inserting into the red blood cell membrane, binding to the 4.1 protein, and forming a membrane fusion channel.