To further confirm the effectiveness of DIPT-ICF NPs in long-circulating angiography for big laboratory animals, circulating retention assessment of angiography were carried out in the rabbit models. The DIPT-ICF NPs was injected via the left ear marginal vein into the rabbits (0.3 mg/mL, 1.5 mg/kg), the serum sample was then collected at various time points to record the luminescence signal (Fig. and). The circulating half-life time of DIPT-ICF NPs similarly is much longer than that of ICG in the rabbit blood circulation (4068 min vs 3.8 min, Fig. ). In vivo angiography experiment shows that stable and strong luminescence of DIPT-ICF NPs in ear vasculatures was observed clearly even over 2 days (Fig. ). While the signal of ICG rapidly disappear within 12 min (Fig. and). Moreover, the SBR (defined as the vessel-signal-to-skin ratio) of DIPT-ICF NPs was significantly higher than that of ICG (Fig. ). The excellent performance of DIPT-ICF NPs inspire us to further explore its real application for intraoperative renal angiography and assessment of renal graft reperfusion in rabbit models.