Along with the R->TS path, when the R->P interactions are compared, it is seen that the active site of the product is stabilized more in the system with Mn 2+ (𝐸 𝑁𝐵 𝑃𝑟𝑜𝑑𝑢𝑐𝑡-𝑅𝑒𝑎𝑐𝑡𝑎𝑛𝑡 = -33.7 𝑘𝑐𝑎𝑙/𝑚𝑜𝑙) ions when compared to the system with Mg 2+ (𝐸 𝑁𝐵 𝑃𝑟𝑜𝑑𝑢𝑐𝑡-𝑅𝑒𝑎𝑐𝑡𝑎𝑛𝑡 = -19.3 𝑘𝑐𝑎𝑙/𝑚𝑜𝑙 ). T1053 (22.4 kcal/mol) shows strong favorable interactions to the active site of the reactant in the system with Mg2+ ions whereas L1218 (18.0 kcal/mol) shows strong favorable interactions to the active site of the reactant in the system with Mn 2+ ions. When looking at favorable interactions with the active site of the product, residues R852 (17.6 kcal/mol), R924 (22.7 kcal/mol) and T1160 (24.9 kcal/mol) show strong interactions in the system with Mg 2+ ions, whereas residues A767(21.6 kcal/mol), R924(19.5 kcal/mol), T1160 (27.6 kcal/mol) and R1190 (33.9 kcal/mol) show strong interactions in the system with Mn 2+ ions. It is interesting to note that most residues with favorable interactions to the product seem to be positively charged, that might indicate stabilization of the charge formed in the active site due PPi. Other residues with considerable interactions (E = |10 kcal/mol|) are shown in table 2. Most of the residues that have considerable interactions in the reaction path are highly conserved evolutionarily, and most are frequent sites for mutagenesis that can cause various diseases.