We applied to the C profiles non-negativity and closure to make the sum of the component fractions for each dataset (at each pH), and we forced the CCS distribution of components (S T profiles) to obey a Gaussian shape. The number of components used to perform the MCR model was first suggested by the SVD analysis and was most often two or three. However, in all cases, MCR models with 2 components, 3 components, and, sometimes, 4 components, were tested. The final number of components was adopted as that providing a good model fit and meaningful resolved profiles. MCR models with a larger number of components were discarded when they provided meaningless profiles and were not improving significantly the fit. The difference in collisional activation necessary for unfolding is larger for the IC (no hairpin) than IH (with hairpin). For IH, the difference with CH becomes higher at high z. The fact that IC appears more kinetically stable in the gas phase than IH is a surprising result, that seems to contradict the memory of the solution phase structure. However, in the presence of the hairpin, the unfolding path is more complicated: for example, it has 4 components at low charges and the components blend at higher charge. Thus, adding the hairpin secondary structure to the i-motif may change the unfolding pathways. When different pathways are characterized by different activation entropies, one can not necessarily expect that V50 or Vinfl values will reflect the degree of hydrogen bonding, which would translate into a change in activation enthalpy. This is one of the caveats in interpreting CIU data in terms of hydrogen bonding content.