LC-MS experiments were performed using a Waters Acquity iClass/Synapt G2S mass spectrometer in positive electrospray (ESI) ionization mode and with ion mobility spectrometry (IMS) enabled. The scan time was 0.1 s over an m/z-range of 50-1200. An MS E method in resolution mode was set up that uses a transfer collision energy ramp from 15 to 40 eV for the high energy MS E data. The following ESI settings were used: capillary voltage = 0.5 kV, sampling cone = 30 V, source offset = 80 V, source temperature = 130 °C and desolvation temperature = 550 °C. Source gas flows were: cone = 50 L/h, desolvation = 950 L/h and nebulizer = 6 bar. For the IMS, the velocity and height of the step wave were set at 300 m/s and 10 V, respectively. IMS wave velocity and height were set at 650 m/s and 40 V, respectively, and IMS gas flow was set at 90 ml/min. Separation was performed using a Waters Acquity BEH phenyl column (100 × 2.1 mm i.d., 1.7 μm particles, 130 Å pore size) and the mobile phase consisted of 10 mM ammonium acetate in H20/ACN 95:5 [v/v] (mobile phase A) and ACN/MeOH 80:20 [v/v] (mobile phase B). The column was held at 40 C° and a flow-rate of 0.4 ml/min and a sample injection volume of 2 µl were used. After an initial time of 0.75 minute at 95% A, a gradient of 6.25 min was run to 95% B, followed by a hold at 95% B for 2.0 min. The column was equilibrated for 1 min between injections.