Glycolipids play a crucial role in AMP interactions due to their large, polar headgroups. Bacterial glycolipids include mono-, di-, tri-, and tetra-glucosyl-diglycerides, as well as glycero-phospho-diglucosyl-diacylglycerols (GPDGDAG) . Glycolipids are essential for cell morphology and biofilm formation . Their abundance varies, with species such as Streptococcus pneumoniae and Enterococcus faecalis exhibiting high levels of glycolipids (> 50%) like di-glucosyl diacylglycerols and GPDGDAG . GPDGDAG also cooperates with lysyl-PG in the development of Daptomycin resistance in Enterococcus faecium strains by forming interactions between the phosphate of GPDGDAG and lysyl-PG amino groups. Moreover, GPDGDAG is an important precursor of wall teichoic acids , highlighting the role of glycolipids as an important linking element between the Gram-positive membrane and the cell wall. Phosphatidylcholine (PC), a zwitterionic phospholipid, comprises a choline moiety (positively charged), a phosphate group, the glycerol moiety, and two acyl chains. Lyso-Phosphatidylcholines (lyso-PCs) derive from PC lipids by removal of an acyl chain . Although PC and lyso-PCs are rare in bacteria, some Gram-positive species like S. pneumoniae, S. pyogenes and S. agalactiae can scavenge PC-derived metabolites, to feed the glycerophosphocholine (GPC) pathway and synthesize PC lipids . Joyce et al. hypothesized that PC lipids may help bacteria evade immune recognition, thus modulating host-pathogen interactions . Furthermore, plasmalogens can function as antioxidants, and thanks to their highly unsaturated tails, they can potentially aid bacterial survival in oxidative environments such as during phagocytosis .