As before, panel 4a shows the amount of C4 beads between the bilayers to measure the time of pore closure, now in the presence of extra-organelle SMACs exclusively. Analogously, figure shows SMAC:lipid contacts (<0.5nm). It can be observed that in all cases (simulations #7 to #12) the fusion pore collapses before 1.5µs while the number of SMAC:lipid contacts keeps increasing (#9 to #12) until saturation (#7 and #8). Interestingly, the steady state regime in panel 4c correlates to the fusion pore closure time. Simulations #7 and #8 (magenta and orange curves) show the fastest increase of SMAC:lipid contacts and are also the first ones where the pore collapses. On the contrary, simulations