The reline and aqueous reline solution have important roles to modulate the solvation and stabilization of large macromolecules like protein, nucleic acid, polymer, etc. For example, (i) aqueous reline solution can prevent the unfolding of HP-36 by urea, (ii) it can stabilize the quartet DNA conformation 107 and (iii) it also helps in the solvation of PEG 400 105 . The understanding of the role of hydrogen bond acceptor and donor in deep eutectic solvent (DES) from this study can motivate the design of more efficient DES for specific purposes by modulating the chemical nature of the constituents and their compositions.