Lyotropic lipid cubic phases formed by monoolein offer promise as host media for enzymes in biomedical device applications. LCPs deposition onto an electrode and its doping with an amphiphilic redox shuttle imparts electrochemical properties to the material. When a redox enzyme is entrapped within the lipid/redox shuttle structure, effective enzyme-based bioelectrodes are generated, for direct application onto transducer surfaces in novel biosensing applications. This protocol is efficient and cost-effective, simplifying electrode fabrication processes, and enabling the storage of bioelectrodes at room temperature.