This study investigated AA usage in two different catchment areas, and results have demonstrated similarities in trends of AA usage in both Bath and Keynsham across the sampling period. They have also indicated, even with their close proximity, that more AA is consumed per person in Bath than in Keynsham. A similar observation has been identified by previous work in the group done within the catchment area, identifying human population size as a key driver of AAs in the environment . This study aimed to further build upon knowledge in the catchment area and results have demonstrated some key findings: