The PTEN/PI3K pathway is involved in forming normal blood vessels and tumor angiogenesis . The PI3K-PKB/Akt-Pten pathway in endothelial cells is required for cardiovascular development, and loss of Pten-mediated regulation of this system can promote tumor angiogenesis . The PtdIns(3,4,5)P3 in cytoplasm is the optimal substrate for PTEN . PTEN dephosphorylates PIP3 via its lipid phosphatase activity. PTEN acts as a negative regulator of the phosphoinositide 3-kinase (PI3K)-PKB/Akt pathway, exerting a tumor suppressor effect . By decreasing PTEN activity in glomerular vascular endothelial cells (EC), TIMAP promotes PI3K-dependent Akt phosphorylation . Inhibition of PKC inhibits ANDV-directed vascular endothelial cell (EC) permeability and alleviates edema in HPS patients . PtdIns P3 is a phospholipid found on the plasma membrane that rapidly increases the concentration in response to the physiological stimulus. Increased PI P3 results in an increased fraction of short-lived clathrin-coated tiny pits (CCPs), a phenomenon that PTEN deficiency . So phosphorylation of PI P3 by the E inhibited the PI3K signaling pathway In summary, the hantavirus proteins exhibited PTEN activity, which was required for the virus entry pathway. PtdIns(3,4,5)P3 dephosphorylation by hantavirus protein facilitated the virus protein's binding to the cell membrane independent of Ca 2+ . However, it would impair lipid metabolism and endothelial cell remodeling in the vascular endothelium cells. It increased vascular permeability and resulted in kidney and cardiac syndrome. We employed the domain search method to investigate the PTEN active domain of the Hantavirus protein in this present study. The findings revealed that Hantavirus membrane glycoprotein E contained intact lipid phosphatase domains and a C2-like domains. Hantaan and Andes had slightly different lipid phosphatase domain characteristic motifs HCXXGXXR. It may be a significant factor in the development of kidney and cardiopulmonary syndromes. 2. Tensin Related sequences. The Tensin related sequence was downloaded from UniProt data set. Keywords is "Tensin".