According to the probes predicted by PU-EPP, we selected 10 potential ZEA hydrolases (ZH 1-10) and 10 potential OTA hydrolases (OH 1-10) for downstream experiments. The candidate genes were synthesized and then expressed with a CFPE system that only required a few hours to synthesize one protein. Then, the obtained enzymes were incubated with ZEA and OTA for 3 h. Overall, 75% of predicted enzymes exhibited expected activities. Nine enzymes exhibited prominent catalytic activities toward ZEA, of which ZH4 eliminated >90% and ZH9 eliminated ~100% of the ZEA content . Six enzymes exhibited obvious catalytic activity toward OTA. Among them, OH1, OH4, and OH6 eliminated >90% and OH8 eliminated nearly 100% of the OTA content in the reaction system . To confirm that the enzymes did catalyze the expected degradation reactions, the metabolic products of ZEA and OTA degradation in the reaction system were measured using high-resolution mass spectrometry. A catalytic product of ZEA with an m/z [M-H]-291.16 was detected in samples of ZH1-ZH9 (Fig ), while the catalytic product of OTA, ochratoxin α, with an m/z [M-H]-255.0067, was detected in samples of OH1, OH2, OH4, OH6, OH8, and OH9 , verifying the predicted degradation reactions.