Incorporating polar side-chain functionalization into the CPP ring may enhance the macrocycles' utility in biosensing and bioimaging applications. Cell imaging and in vitro antitumor activity studies were performed to evaluate the biological potential of the bismacrocycle 5. Fluorescence imaging with the HeLa cervical cancer cell line was conducted to assess the bismacrocycle's cellular uptake. After 90 minutes of incubation, weak green fluorescence was observed within the cells, indicating successful penetration of the cell membrane (Figure ).