Immobilization of QACs, Anti-CRP mAb, and BSA Blocking. NR3 + PS beads (5.0 mg/mL) were mixed with anti-CRP mAb (7.6 μM) (or anti-NP mAb (6.6 μM)) in a 1:1 volume ratio. Anti-CRP mAb was bound to the beads in the prepared solution. The solution also contained unbound anti-CRP mAb. As the GFET array was divided into two regions by silicone rubber, the two regions were individually functionalized. The mixed solution was dropped onto Region 1 and incubated for 0.5 h at room temperature. Through this process, QACs and mAb were decorated on the GFETs. Region 2 was incubated with 0.01×PBS, which was not functionalized. After incubation, both regions were washed with 0.01×PBS and subsequently treated with 1% BSA in 0.01×PBS for 0.5 h to block non-specific adsorption. After washing, the chip was ready for biosensing. The GFETs in Region 1, where the mAb was immobilized, are referred to as GFETssig, and those in Region 2, where no mAb was immobilized, are referred to as GFETsref. These can be flipped if the mAb is immobilized in Region 2.