Including PARK7 and GAPDH, 78 proteins have substantially increased (fold change > 2 and q-value < 0.1) affinity for DNAJB8 following propachlor treatment (Figure ). This is more than the twice the combined number of proteins destabilized under the same criteria after alachlor and acetochlor treatments. The higher susceptibility of the proteome to propachlor could be based on substitution reaction reactivity. Kinetic studies between propachlor and alachlor reactivity found a 2-fold increase in the substitution of propachlor against several nucleophiles and a lower Gibbs free energy required for substitution reactions of propachlor with nucleophilic thiols . All three treatments cause marked destabilization of TYMS and ACAT1, and apparent stabilization (decreased DNAJB8 affinity) of CEPT1 and MGST3 (Figure ).