A dose response curve was generated for the half-strip LFA using irradiated virus in pooled nasal matrix. The limit of detection was determined to be 23 TCID50/mL (95% CI of 9.1 to 37 TCID50/mL), equivalent to 1.4x10 5 copies/mL (95% CI of 5.5x10 4 to 2.3x10 5 copies/mL) for the lot of irradiated virus used, and was calculated in R using the drc package. For point of care test use cases, the WHO has proposed a minimal LOD of 10 6 genomic copies/mL and an optimal of 10 4 genomic copies/mL. The LOD for this assay, as tested, falls between these guidelines, and is superior to the analytical sensitivity of the commercially available antigen tests available as of early August, 2020.