Total loads of AAs and metabolites in wastewater were generally higher in winter when compared to summer (figure ). Statistical significance was gauged using T-tests to compare the three seasons for which most data was available; winter 2018/19, spring 2019 and summer 2019 (table ). SPY had one of the lowest temporal variabilities across the three seasons in both Bath and Keynsham (average loads, winter: 47.1 ± 17.6 and 6.6 ± 2.9 g/day, spring: 39.6 ± 15.8 and 5.0 ± 3.8 g/day, summer: 43.5 ± 16.9 and 6.7 ± 2.9 g/day for Bath and Keynsham respectively, all p>0.05). In Bath, SMX also had low temporal variability across the three seasons, with 14.7 ± 5.0 g/day, 14.3 ± 5.7 g/day and 15.5 ± 4.8 g/day in winter, spring, and summer respectively (on average a 5 % change between seasons, p > 0.05).