All samples were randomly injected with the method 3 in two batches. Analysis of the first batch consisted of the 40 oxPUFA samples, a pooled quality control sample (QC), which was prepared mixing 5 µL of each oxPUFA sample, and a blank sample both injected each 10 th samples. Different DDA inclusion lists were made for each PUFA oxidation calculating the theoretical molecular formulae after adding different levels of oxidation following the known mechanisms of PUFA oxidation. The inclusion lists are available in Supplementary Table . The second batch contains the microscaled oxidized 15-HETE, and DMSO-rearranged PGH1, and PGH2. Analysis of blood serum and microalgae samples by LC-MS/MS DDA. The processed blood serum and microalgae samples were injected into the LC-MS/MS system following the method 3. A combine inclusion list of the four different inclusion lists (per PUFA) was used.