Several E. coli colonies were streaked to inoculate 2 ml of starting media (2xLB supplemented with 0.5% glucose and 100 μg/ml ampicillin) in a 24-deep-well plate (GE Healthcare, UK). The plate was covered with an air-pore membrane and incubated at 37°C for 4 hours, 200 rpm. After incubation, 2 ml of induction media (2xLB supplemented with 0.6% lactose, 50 mM HEPES (pH 7.4), 0.5 mM IPTG, and 100 μg/ml ampicillin) was added. The plate was covered with an air-pore membrane and incubated at 22°C for 16 hours, 200 rpm. Cells were harvested by centrifugation using Sigma 6K-15 centrifuge (SciQuip, UK) for 10 min, 1519 g, and 4°C, and resuspended in 1.3 ml of a purification buffer (16.4 mM K2HPO4, 3.6 mM KH2PO4, 400 mM NaCl, 10 mM imidazole, pH 7.5). After cell disruption (Sonic Dismembrator Model Q700S, FisherBrand, USA) the whole soluble fraction was clarified by centrifugation for 20 min, 3572 g, and 4°C. Soluble fraction was added to TALON SuperFlow Metal Affinity Resin (Takara) pre-equilibrated with sterile water and incubated for 2 hours on a roller (40 rounds/min) at 4°C. Unbound proteins were washed twice by and centrifuged at 94 x g for 2 min followed by resuspending in a purification buffer. After the second wash, 40 µl of SDS-PAGE loading buffer (2x Laemmli Sample buffer containing DTT) was added to each protein/resin sample. Samples and a marker (Color Prestained Protein Standard, Broad Range 10-250 kDa, New England Biolabs, USA) were loaded on SDS-PAGE gel with run conditions: 400 mA, 200 V, 40 min. After staining with InstantBlue™ (Missouri, USA) for 20 min, the gel was washed with water for 40 min.