The thermodynamic profile of the interaction between COSAN and DNA was assessed by ITC. For ITC, COSAN (5.0 mM) was titrated into 1.0 mM (pb) ctDNA solution. The resulting ITC profile was corrected by subtracting the corresponding dilution heats derived from COSAN injections into the buffer (Figure ). The plot of the measured enthalpy values as a function of the [COSAN]/[ctDNA(pb)] ratio shows a random distribution of enthalpy values around zero kJ/mol (Figure ). The mean enthalpy per injection was -0.11315 ± 0.18784 kJ/mol, which shows that COSAN titration into ctDNA shows no heat effect, thus indicating that COSAN does not interact with DNA.