Therefore, the maximum velocity, Vmax, is the asymptotic kind because it is ≫ the mixed order maximum velocity (𝑉 max prss ), where the [S0] ranges between values that are ≪ the Michaelis-Menten constant, KM, and values <KM. Let it be made clear that while M2 is definitely the molar mass of the enzyme if known ab initio, the molar mass of the substrate cannot be definitely represented by MS for reasons that are germane in the light of total Preprint submitted to ChemRxiv, Jun 05, 2023