This study successfully demonstrated a biocompatible, SANs-functionalized, 3D-printed hollow microneedle-based electrochemical sensor for wireless, continuous and real-time glucose monitoring in interstitial fluid. The integration of biocompatible HMNs and a finger-activated pump exhibits the potential for minimally invasive sampling of ISF. The SANs-boosted electrochemical sensor highlighted high sensitivity and remarkable selectivity, while the wireless potentiostat enabled user-friendly, real-time and continuous monitoring of glucose levels. In the proof-of-concept, a skin-mimicking phantom gel was used to validate the electrochemical sensing capability and sensitivity "in vivo". Our integrated wearable sensor provides a feasible solution for continuous and precise glucose monitoring, representing the potential for health monitoring. Future studies will focus on refining the fabrication techniques to develop miniaturized devices and optimizing material properties for enhanced sensing performance. Additionally, we aim to expand our device's capabilities to monitor multiple biomarkers for comprehensive health management. With these advancements, we believe our proposed work is promising to notably contribute to the development of the next-generation wearable devices, paving the way for personalized healthcare.