In 2024, Wu et al. advanced the Single-probe for ambient liquid extraction MSI studies aimed at enhancing the detection of poorly ionizable lipids in brain tissue using a Lewis acidic metalorganic framework (MOF) . In this study, the sample was placed on a triaxial platform, with the Single-probe affixed in a perpendicular orientation relative to the sample surface. The team employed 1% FA-MeOH as the extraction solvent at a flow rate of 5 μL•min⁻¹, delivered via a syringe pump. A vacuum pump was used to draw the solvent into the probe, creating a stable liquid junction with a precise 10 μm distance between the probe tip and sample surface. This approach effectively mitigated ion suppression by phospholipids in MSI, significantly improving the detection coverage of low-abundance, poorly ionizable lipids.