A "bird's-eye" view of the physicochemical properties of proteoforms identified in the five different tissue types, including hydrophobicity, monoisotopic mass, and pI value, can be found in Figure and. While kidney, lung, and spleen tissue proteoforms show similar distributions in their violin plots regarding all three investigated characteristics, distinct differences for heart and especially small intestine tissue were detected. For example, in the case of the small intestine, a high number of proteoforms in the pI range of 10.5 to 12.0 was observed, which can be explained by a relative increase in histone proteoforms compared to the other analyzed tissue types. This is also supported by the negative GRAVY score, showing a large distribution at around -0.6. On the other hand, proteoforms observed in heart tissue exhibit a relatively broad distribution of pI values.