Sample handling and pooled quality control (QC) sample preparation were done in accordance with guidelines previously reported . Samples were analyzed by the two complementary C18+/-and C8 RPC+/-methods. The pooled QC sample was used for data quality monitoring and feature filtering. To assess the metabolome coverage, targeted extraction, and integration of annotated metabolites in the generated datasets was done using the R package peakPantheR . Further details of data preprocessing and analysis are described in Experimental S2.