To validate the Martini 3 plasma membrane model, we built and simulated a smaller version of the membrane presented in Figure ,A and simulated it for 20 µs with the Martini 3 force field, followed by a conversion to an all-atomistic representation compatible with the CHARMM36m force field (Figure ,B) using ezAlign , which was then simulated for 100 ns. The structural properties of the CHARMM36m plasma membrane model are comparable to the Martini 2 and 3 models (Figure ). The membrane thickness is 4.19±0.002 nm and is closer to that of the Martini 3 model. The area per lipid values leaflets of the CHARMM36 model are found to be 0.514±0.005 nm 2 in the extracellular and 0.585±0.006 nm 2 in the cytoplasmic leaflet. Similar to our Martini simulations, using CHARMM36 results in an extracellular leaflet rich in cholesterol and lipids with saturated tails having a lower area per lipid than the PUFArich cytoplasmic leaflet.