A 1.00 mg/ml stock solution of betamethasone 17-valerate standard was prepared by dissolving 0.100 g of standard in absolute ethanol and diluted to 100 ml with the same solvent. A 1.0 ml of 1.00 mg/ml was pipetted in a glass beaker and diluted to 10 ml with absolute ethanol to make a 0.1 mg/ml solution which were serially diluted to make concentration of 0.006 mg/ml, 0.008 mg/ml, 0.010 mg/ml, 0.012 mg/ml and 0.014 mg/ml solutions. The standards were prepared using BV secondary standard after discovery that both primary and secondary standards have similar absorption spectrums.