Generation of several conformers with different orientations suggests flexibility of the UBP inside the DNA duplex. Higher RMSF for DS and DN further confirms the flexible nature of the UBP (Figure ). RMSD values of the entire DNA are found to be synchronized with conformational orientations of the UBP; analogous to what is observed with the AMOEBA force field. Here also elevated RMSD values correlate with distorted structures whereas nWCFP1 And WCF structures exhibit comparatively lower RMSD values (Figure ). It is noticed that conformational change of the UBP is reflected on the associated geometrical parameters related to the UBP depicted in Figure and S4.