To investigate the energy transfer process between the B-fiber and RhB, the photophysical properties of the donor (B-fiber) and acceptor (RhB) were measured. With an increased concentration of RhB (0 to 200 μg/mL), the emission spectra of the B-fiber/RhB system (λex = 270 nm) exhibited a gradual reduction of phosphorescence emission, centered at 490 nm.