DLS was used to record the mean centred hydrodynamic diameters for unfunctionalised gold nanoparticles (13 nm diameter, 0.50 PDI), Sequence-A (17 nm diameter, 0.12 PDI) and Sequence-B (12 nm diameter, 0.14 PDI) functionalised gold nanoparticles and gold-aptamernanoconstructs (1154 nm diameter, 0.36 PDI), Figure . Unfunctionalised, Sequence-A and Sequence-B functionalized gold nanoparticles exhibited comparable particle size distributions. The subtle changes in mean hydrodynamic diameter observed by DLS could be attributed to differences in gold nanoparticle bound nucleic acid chain composition, length and corresponding ionisation (Figure , Sequence-A 8442.7 Mw, Sequence-B 4241.9 Mw and Sequence-B-Biotin 4811.5 Mw). However, when Sequence-A and Sequence-B functionalised gold nanoparticles were aggregated with the aptamer the mean centred hydrodynamic diameter, as measured with DLS, was considerably larger (50x), which alongside colourimetric and spectroscopic characterisation, confirmed gold-aptamernanoconstructs were fabricated. The relatively large particle size distribution associated with gold-aptamer-nanoconstructs suggests there is heterogeneity during aggregate production, such that their hydrodynamic architecture can span greater than 1 µm in diameter.