The former endpoint can be used to analyze how a perturbagen, here: a small molecule, might perturb the regulatory pathways. Investigators can adjust the bioactivity cutoff to only take into account compound-target interactions that are more potent than the set value. This feature of the SmartGraph API has direct relevance to studies aimed at elucidating mechanism-of-action in the context of phenotypic screening, as well as designing multi-target therapies.