To further investigate the spatial inter-and intra-droplet heterogeneity of lipid and protein oxidation, we used CAMPO-AFDye 647 to localize protein radical formation and BODIPY 581/591 C11 for mapping lipid oxidation. This blue-shifted alternative BODIPY dye does not overlap with the emission of CAMPO-AFDye 647, which enabled us to co-localize protein and lipid oxidation. BODIPY 581/591 C11 has the same core structure as BODIPY 665/676 but contains only one phenylbutadiene moiety, which makes it less sensitive to lipid oxidation. Co-localization of lipid and protein oxidation in polydisperse emulsions is shown in accumulation at the interface, suggesting that proteins at the interface oxidize heterogeneously at both the interand intra-droplet level (Figure &). Heterogeneous protein distribution induced by colloid mill might make droplets more susceptible to lipid oxidation compared to the emulsions which have homogenous distribution of proteins at the interface. This finding could be explained by the heterogeneous interface of droplets featuring areas without proteins that could increase the chance of the lipids to react with AAPH in the aqueous phase.