Both IDT types require high input frequencies in the MHz domain which can lead to the development of heat in the device. In particular, the thermal contribution in operating microfluidic SAW devices has been shown to evaporate fluids in digital microfluidics and has negative impacts on the rheological properties of suspension media, in vitro protein stability, and cell viability . For example, many systems that operate in the MHz frequency domain restrict exposure time of biological samples to the acoustic field or implement complicated feedback temperature control strategies including Peltier stages to mitigate thermal contributions. Other works attempt to exploit device heating by using the phenomena in applications such as continuous flow polymerase chain reactions or to deliberately kill cells . A simple, integrated droplet-based acoustofluidics device that is mechanistically characterized and acoustically manipulates droplets with physiologically relevant conditions has yet to be shown.