We reliably and reproducibly detected complete perfusion of the injected D 2 O in the water-soaked 1 % CMC cryogel scaffolds, again performing manual injections and varying controlled flow rates with a syringe pump. Compared to the experiments performed with manual injections in the PEGDA hydrogel, when using a 1 % CMC cryogel, all the D 2 O injected perfused, removing all the water from the scaffold and placing this at the top of the well. We can observe this process in the proton images from Fig. and confirm the absence of proton signal after injection by looking at the horizontal pixel sum of each well shown in Fig. . Despite having four replicates per experiment (four independent wells in the chip), we repeated this experiment three times to ensure the perfusion efficiency and repdroducibility of the cryogel tested. As we indicate in Fig. , the results were consistent at each trial, forcing all the water proton signal to get out of the scaffold into the top of the well after the injection of D 2 O.