Clinical samples were pooled to conduct screening in Round 5 (Table ). A 1:100 titration was confirmed to produce visibly weak signal intensity at the test line and was therefore used as the positive control antibody pair SiB-MM08 / SiB-R004 (capture antibody / detection antibody). Aliquots of 1:100 clinical pooled samples were prepared and stored at -80°C until thawed for a single experimental use, then discarded.