Here, we fold dsDNA scaffolds of up to 18000 nt (9 kbp). While other DNA-based methodologies have yielded even larger non-periodic structures , triplex origami is the first to require fewer unique starting materials and thus substantially decreases the cost-of-entry to this assembly size. The work presented here ensures that each staple recognition domain is sequentially unique by a margin of at least two mismatches, in addition to a number of other assumed restrictions on sequence design. There is room for improvement for the sequence design methodology, as it currently has not involved any refined optimization steps. Furthermore, there is mounting evidence that scaffolded self-assembly permits a surprising extent of sequence redundancy, without it affecting the yield. These boundaries must be explored before we can quantify the size limit of triplex origami structures. Assuming that one mismatch is sufficient to retain specificity, patterned structures from scaffolds of >100 kbp may be formed with the presented methodology.