Herein, kinetics parameters, Km and Vmax, of oxidase-like Ce/Fe-bimetallic nanozymes toward 3,3′,5,5′-tetramethylbenzidine oxidation were determined. The Michaelis-Menten kinetic model was used for the evaluation of the kinetic of the nanozyme-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine. To provide the quantitative and accurate values of kinetics parameters, Km and Vmax, the linear plot of Lineweaver-Burk was constructed. The results exhibited a Vmax as high as 67.56 nM min -1 for the oxidase-like Ce/Fe-bimetallic nanozymes.