The cured particles with a smaller hydrodynamic radius (HL85NP, 60 nm) showed a faster color change compared to the ones achieved with larger particles. This color change was an indication of reduction of silver ions. Regardless of the particle size of cured HLNPs, there was a clear and time-dependent increase in the absorbance caused by the SPR of silver at 407 nm for HL85NP and 420 nm for HL60NP (Figure ). To further elucidate the effect of HLNP particle size on the size of final self-assembled silver nanoparticles, we studied silver-lignin hybrid particles by DLS. The particle size distributions showed a clear increase in hydrodynamic diameter upon silver reduction in HL60NP and HL85NP dispersions (Figure ). Unlike in the case of the smaller particles (HL85NP, d = 60 nm), there was only a minor broadening in the particle size distribution of larger particles (HL60NP, d = 112 nm). This difference indicates differences in reaction kinetics and surface coverage of the particles by metallic silver. nanoparticles are assembled on the particle surface. c) DLS particle size analysis of HLNP before and after of silver nanoparticles self-assembly on the surface. d) UV-Vis spectra of silver nanoparticles formed on HL85NP surface with 60 nm diameter (data shown for every 10 min here). e) UV-Vis spectra of silver nanoparticles form on HL85NP surface with 110 nm diameter (data shown for every 10 min here). f) UV-Vis kinetics plots of silver nanoparticles self assembly on HL85NP with different particle sizes. g) TEM image of HLNPs reacted with silver ammonia solution. h) Silver nanoparticles formed on the surface of HLNPs with yellow arrows pointing on them, i) Calibration curve for detection of hydrogen peroxide (H2O2) using HLNPsilver hybrid nanoparticles.