Using simulation systems with clear, easily identifiable phases present (such as Lb and Ld, Figure ), thresholds were identified to determine the phase of each individual lipid. If the peak height was below the lower threshold, a lipid was assigned in the Ld phase. Conversely, if the peak height was above the upper threshold, a lipid was assigned in the Lb phase. If the peak height was between the two thresholds, a lipid was assigned in the Lo phase. Using the information from this analysis, the properties and phase assignments for each lipid were plotted on the final positions of the lipid tails and inspected to determine which phases were present. An example of this calculation is illustrated in Figure for the system 60% DPPC, 40% DOPC, 0% CHOL.