Gnanasekaran et al. investigated the effect of solvent (water vs. dimethylformamide), electron flux or electron dose rates, and imaging mode such as (parallel vs convergent probe) on the radiolytic behavior of ZIF-8 nanocrystals during LC-TEM experiments . They noticed radiolysis damage is more significant when imaging in TEM mode, particularly when water is used as the solvent. They found that ZIF-8 nanocrystals can lose their crystallinity before any visible morphological change is seen during in-situ imaging; hence, maintaining morphology doesn't always indicate an intact crystalline structure under electron beam irradiation. By measuring changes in the {110} Bragg plane, they reported that ZIF-8 nanocrystals lose their crystallinity at a total dose of around 80 e -Å -2 .