The MS images obtained from the Single-probe MSI experiments can be integrated with fluorescence microscopy images through image fusion (Figure ). In Alzheimer's disease (AD) research, the Single-probe was used to investigate the spatial distribution of metabolites around amyloid-beta (Aβ) plaques in an AD mouse brain . Image fusion allowed researchers to correlate histological markers (detected through fluorescence microscopy) with metabolomic features (observed through MSI). This combined approach improved spatial resolution (~5 μm) and provided insights into abnormal metabolite expressions, such as lysophospholipids, malic acid, and glutamine, that are linked to the progression of AD.