A total of 49 compounds were tested in this study. Three ligands that altered the fluorescence level of the tubulin and coumarin-30 mixture by more than 20% compared to the control in the initial fluorescence scan were excluded from the screening. The C-MST assay outputs of the remaining ligands were analyzed using the one-sample Z-test. The true standard deviation was estimated based on the entire set of measurements for 46 remaining compounds. Signals that were statistically different from the control (two-tailed p-value < 0.05) were considered positive hits.