Calibration was conducted on 12 polystyrene standards with masses (Mp) in the range of 474-1800,000 g/mol. Measurements were performed at 35 °C. Chloroform (CHCl3) HPLC grade with a flow rate of 0.7 ml/min was used as the mobile phase. The specimens with a concentration of 3 mg/ml were filtered through a PTFE membrane with a 0.2 µm pore size before measurement. Data were recorded using the "ChemStation for LC" program and analyzed using the "ChemStation SEC Data Analysis Software".