While cooperativity measures changed only marginally for the different θ B,o and φ B,o considered, stability of the designed helix varied significantly with θ B,o . The effect of the bond-bending angle on stability dominates the effect of torsion angle for the parameter ranges considered here. Figure shows the heat capacity and native contact fraction curves for varying equilibrium bond-bending angle values, with a backbone torsion equilibrium angle of 16 • , and Figure shows the melting points and characteristic widths from both heat capacity and native contacts curves for the complete 2D scan of θ B,o and φ B,o . As may be anticipated, the melting point is highest for the foldamer with angle parameters closest to that in the designed helix (see Table ). and native contacts curve (bottom left) reveal that the (i to i + 5) helix is less stable than the (i to i + 4) helix. Heat capacity full-width half-maximum (top right) and sigmoid width parameter (bottom right) indicate that cooperativity is not significantly different for the (i to i + 4) and (i to i + 5) helices. In addition, the effect of perturbing the backbone torsion angle on melting point and characteristic width of the transition region is negligible over the parameter range tested. For the native contact sigmoid parameters, the data point for φ B,o = 12 • , θ B,o = 120 • , has been omitted due to the non-sigmoidal behavior of the native contacts curve. All uncertainties are estimated as the standard deviation from bootstrapping.