Interestingly, 100% methodology is ubiquitous in hyphenated LC-based quantitation. Thus, there is no reason why the well-established and easy-to-use 100% "mass balance" quantitative evaluation method (i.e., integration of all visible peaks and determination of their ratio on a 100% basis) can be the gold standard in (U)HPLC and its many derivative methods, but could not be used equally widely in qHNMR. In fact, the 100% qHNMR method should be considered more seriously as a very practical method that can be readily applied to almost any routine qualitative H NMR data set. Practicability of 100% qHNMR Fingerprint Analysis. NMR addresses the major limitation of HPLC quantitation with its need for calibration curves, which require the availability of identical reference standards for each target analyte. As an orthogonal method, qNMR methods offer both absolute (EC and IC qHNMR methods; abs-qNMR) and relative (100% qHNMR method; rel-qNMR) quantification for purity and integrity analysis. The advantage of the applied 100% qHNMR method is that it is both untargeted and independent of identical reference standards. Notably, it does not necessarily require knowledge of the particular mass of a detected molecular species as it measures the relative molarity. Moreover, it does so with a unity response factor, independently of knowing the mass and/or identity of the impurities. In the present study, data processing was performed using a previously optimized approach for qHNMR spectra, with the following parameters: a Lorentzian-Gaussian resolution enhancement (LG) with a Gaussian factor of 0.8 Hz and a line broadening factor of -1.5 Hz was applied; all 64k-sized time domain spectra were zero-filled to 512k data points to increase the digital resolution; a 5th order polynomial fit was applied for the baseline correction.