Concentration dependence was also studied for SOµC-Bdpy1 and SOµC-Bdpy2 microchips incubating them overnight and 24 h at 37 °C with different concentrations of GSH (1, 3, 5, 7, and 9 mM in PBS solution), and, then, were analyzed by the fluorescence microscopy (Figure and Figure in the Supporting Information). Figure shows that incrementing of GSH concentration from 1 mM to 9 mM, the fluorescence intensity in the green emission for SOµC-Bdpy1 is gradually reduced followed by the increase in red emission which is also seen by the fluorescence intensity profile plots. Figure also illustrates the ratiometric signal calculations of fluorescence intensity in each concentration of GSH after overnight incubation time. Figure shows a linear trend for the average of ratiometric calculations resulting from the microchips in each concentration of GSH. On the other hand, the experiments with 24 h of incubation (Figure ) revealed increasing in ratiometric signal compared to overnight incubation time at the same concentrations of GSH for SOµC-Bdpy1.