Lipid wash Washing of lipids from tissues was carried out as described by Schober et al . Tissues were submerged in 25 mL 70:30 ethanol:water for 30 seconds twice, then once for 15 seconds in 25 mL 100% ethanol, then once for 10 seconds in 25 mL 90:9:1 ethanol:glacial acetic acid:water, and finally twice for 2 seconds in 25 mL water. They were then dried under a gentle stream of nitrogen.