As the device works by drawing the sample across the RPS orifice and through the exit tube, akin to the reverse of a flow focusing chamber in flow cytometry, it was hypothesised that alongside the enhanced particle counting rate, the additional flow may help wash/clean and unblock the pore mouth if larger aggregates were present in solution. To test this hypothesis, a GNM was placed into the fluidic chamber, the solution filled with 300 nm particles, and the pulse frequencies were measured (figure ). Into this solution, 10 m particles were also placed at a concentration of 1 x 10 7 particles/mL. The presence of the larger particles had no effect on ease, speed and consistency of particle translocations (figure ). The presence of the larger particle did not inhibit or change the measurement of the smaller ones. The larger 10 um particles could still be counted in the same device simultaneously using sensor 1. This ability to measure a wide range of particle sizes, or the properties of smaller ones without the need for sample preparation and filtration, may have many applications in biological and physical sciences. In theory, the addition of a syringe to the back of sensor 2 allows the user to increase the fluid flow through the GNM, by pulling the syringe out, or prevent the convection through the pore by pushing the syringe down. The flow rate through the centre of the pipette was difficult to measure, given its small volume, and the flow rate ratios are not known here. It may be possible to control this further in more advanced systems and is the focus of future work. However as the ratio of flow was changed to allow more liquid through the GMN i.e. the syringe was pulled to draw liquid through the sensor, the pulse frequency also increased (figure ). Conversely, when the liquid flow was reversed back from the GNM into the main channel, no translocation events were observed (no data available to plot in figure ) as a comparison the blockade size, frequency and translocation speed of the particles in the absence of any additional flow from the syringe is also shown in figure .