Inverted fluorescent microscopy image for 24 hours incubation reveal that on the PLLA and PLL-PEG layer HeLa cells were not properly attached. Therefore very low number of cells viability let assume that part of cells, which was not attached to the well bottom was washed out during medium change. However, it does not indicate toxic effect of the layers per se. It could be confirm by microscopy image the dishes with attached electrodes, despite that electrode does not have directly contact with a dish bottom. For TMOS layer the survival of HeLa cells incubated was almost 50%, which was much higher than in other cases (Figure , Figure ). Considering the above, it can be assumed that neither of tested layers are nontoxic to HeLa cells, and can be use as a antifouling material in cell culture medium.