The role of elemental iron in living organisms is implicated in critical metabolic processes such as hemoglobin and myoglobin synthesis, cell proliferation and regulation, and DNA synthesis. About one-fifth of iron in the body is stored in an intracellular protein called ferritin, with seventy percent stored in hemoglobin. Chronically low iron levels either through blood loss, pregnancy, or inadequate iron intake can cause iron depletion over time, resulting in iron deficiency anemia. Iron intake through food fortification or medicinal supplementation has been shown to be effective in preventing and treating iron deficiency anemia. Excess iron intake, on the other hand, has been linked to the etiology of a variety of chronic diseases, including cancer, diabetes, and cardiovascular disease. Ferrous sulfate supplements are often prescribed to prevent or treat iron deficiency anemia. It is administered orally as a liquid, tablet, or capsule. In the context of possible low iron intake or overload, the validation of the actual amount of iron contained in ferrous sulfate supplements is imperative. Trace element analysis of metals including iron has been an integral part of most undergraduate chemistry laboratory experiments. These experiments exploit a myriad of analytical techniques and instrumentation, also providing students the opportunity to work with real-life samples. Cyclic voltammetry offers a versatile electroanalytical technique for both qualitative and quantitative studies of redox active specie. It can be used to determine the diffusional coefficient and reduction potentials of an analyte for qualitative purposes. The concentration of a redox active specie in a Nernstian system is proportional to peak current (Equation ) allowing for the quantitative determination of analyte concentration. 𝒊 𝒑 = 𝟐. 𝟔𝟗 𝒙 𝟏𝟎 𝟓 𝒏 𝟑/𝟐 𝑨𝑫 𝟏/𝟐 𝑪𝑽 𝟏/𝟐 (1) where 𝒊 𝒑 is the maximum or peak current, n is the number of electrons transferred in the redox reaction, A is the area of the working electrode in cm . C is concentration in mol/cm 3 of the electroactive species at the surface of the electrode. V is the scan rate in V/s, and D is the diffusion coefficient of the electroactive species.