A significant limitation of this study is the quality and accuracy involved, and they heavily rely on the algorithms and models used. Inaccurate models may create false positives. To mitigate and prevent this, we validated our docking by checking the experimentally determined and docked structures. For further validation, SPR (surface plasmon resonance) or ITC (isothermal calorimetry) should be performed. These studies will further confirm the binding of the ligands to the protein. Also, a binding analysis requires site-directed mutagenesis and cocrystalline structure to show the amino acids involved in binding. A possible extension of this investigation is to automate the entire pipeline so that we can design and evaluate more peptides in less time. In this research, we have done computational modeling of the CD28-TYRP1 receptor complexes. This research will help in developing CAR T-cells against melanoma.