We analyzed simulated and experimental data. Simulations are advantageous because we know the true values and can repeat calculations many times. For experimental data, even without true values and as many repetitions, it is helpful to compare estimated ASE for different statistical analyses. We analyzed experimental data from the the COVID Moonshot project. ? The COVID Moonshot is worldwide collaboration to develop an open-source antiviral that treats COVID-19 by targeting the SARS-CoV-2 main protease. We analyzed CRC in which protease activity has been measured by fluorescence (1668 curves) and mass spectrometry (238 curves). These datasets can be found in an Excel Spreadsheet as Supporting Information 2.