The HEK293-derived AAV2 samples separated at 25°C and analysed with a resolving power of 45K were used for the development of the PTM monitoring method. This method was created following the same methodology as the development of the rapid identity testing method but optimized for the characterization of the selected serotype (Table ). Here the component target list contained VP PTM modifications specific to the serotype being studied rather than a list of VPs from different serotypes.