The Application layer is composed of repositories and tools that read information from the Databank layer for further analyses. Because the Application layer does not interfere with the Databank layer, it can be freely extended by anyone for a wide range of purposes. This is demonstrated here with two examples: the NMRlipids Databank graphical user interface (NMRlipids Databank-GUI) at databank.nmrlipids.fi and a repository exemplifying novel analyses utilizing NMRlipids Databank as discussed below (github.com/NMRlipids/DataBankManuscript). A more detailed description of the NMRlipids Databank structure is available in the supplementary information.