Peptide aggregation presents both challenges and opportunities in AMP design. Modulating aggregation properties may enhance activity against target membranes or mitigate toxicity to human cells. Moreover, aggregation affects pharmacokinetic profiles, such as half-life and organ distribution , underscoring its importance in overall peptide performance. Molecular aggregation can well be studied with different experimental tools, also for smaller peptide accumulates, e.g. using fluorescence tools such as fluorescence correlation spectroscopy or advances in scattering microscopy techniques such as the weighing of proteins and their aggregates using interferometric Scattering (iSCAT) microscopy [417].