At the current stage of methodology, the extraction of accurate chemical shifts and all relevant spin-spin coupling constants is possible for quite large molecules. Possibly due to the limitations of historic tools such as LAOCN3/LAOCOON (Least-squares Adjustment of Calculated on Observed NMR Spectra, see and references therein), there is a general belief that this type of analysis is limited to 8-12 spins and not useful as a general application. Today, as we have much more powerful computers, several cases demonstrating the feasibility of analyzing molecules with a molecular weight in the range of 1,000 amu and above have been reported, as follows.