Four replicates of 2D-cell cultures of astrocytes were grown in the presence of different natural neuro-inflammatory triggers at different concentrations, namely interleukin 1β (IL-1β) at 30 ng/mL, tumoral necrosis factor α (TNFα) at 30 ng/mL, and lipopolysaccharide (LPS) at 10 µg/mL. The control cell culture was grown in parallel in absence of any inflammatory trigger. After two weeks of growth, cell cultures were snap-frozen in liquid nitrogen. Protein precipitation was achieved by adding 1 mL of a cold solution (-20 • C) of MeOH:H 2 O (80:20 v/v), scraping, and by vortexing during 1 minute. Samples were centrifuged at 14000 g during 15 minutes at 4 • C, the supernatant was collected and then evaporated to dryness before resuspension in 100 µL of a solution made of MeCN:H 2 O (50:50 v/v). Quality control (QC) samples were prepared by pooling the same volume from each sample after reconstitution. Volumes of 10 µL of individual cell culture extracts, QCs and diluted QCs were evaporated to dryness using a SpeedVac (ThermoFisher, Langenselbold, Germany). Before injection, samples were reconstituted with 10 µL of an aqueous solution containing paracetamol, procaine and ethyl-sulfate at a concentration of 50, 5 and 5 µg/mL respectively.