AA-atom MD simulations are performed with GROMACS package. The already equilibrated NM membrane system, obtained from literature is directly simulated at 310K for 230 ns. After short energy minimization using the steepest-descent algorithm, the MMS and MMU systems are equilibrated at 310 K at least for 100 ns followed by production run of 100 ns. We gradually cooled the three membrane systems (NM, MMS, and MMU) from 310K to 250K with the last configuration obtained after production run at 310 K. Cooling is done via annealing with 1 K/ns rate. Then we equilibrated each system not less than 250 ns, as molecules takes longer time to diffuse at lower temperature, hence longer time is required to reach the equilibrium. The temperature is increased gradually at 20 K interval.