or through 2D Voronoi tessellation (APL vor ). Both APL xy and APL vor results indicate that the MMG-6 molecules tend to occupy larger areas in all of the investigated systems (Table , Figure ). Moreover, the effect of interdigitation on the membrane area is clearly demonstrated by the APL xy results: the lipids in the interdigitated double bilayer systems take up approximately 1.7 (MMG-1) to 1.6 (MMG-6) times larger areas in comparison to the corresponding non-interdigitated double bilayer (Table ). Similarly, the APL vor distributions show a shift to higher APL values for individual lipids in the interdigitated systems (Figure ). The APL xy versus time plots can be found in Figure . Only MMG-1 has been reported to assemble into fully interdigitated membranes below 25°C, whereas MMG-6 has been proposed to form non-interdigitated structures at the temperature used for the simulations. In contrast to these experimental results, no apparent difference was observed from the mass density profiles (Figure .3). Both visualizations and the number density profile histograms show that the tails of MMG-1 and MMG-6 extend into the opposing leaflet, forming partially interdigitated regions (Figures S2.4, S2.5). The degree of partial interdigitation is comparable between the analogs, and MMG-6 displays even slightly larger histogram intersections in some cases. However, partial interdigitation is a phenomenon encountered with lipids both in the gel and the disordered phases and does not necessarily predict the formation of a fully interdigitated phase. MMG analogs display differences in tail order, tilt angles, and conformations