The symmetric nature of the peaks suggests the equivalency of the two dihedrals. In contrast, the peaks for 𝜑 9 and 𝜑 11 are not symmetrical for the cyp chains indicating that although these two dihedrals look similar, they are non-equivalent and we find peaks near 90 o and 180 o . With lowering of temperature peak height increases, confirming a more rigidity of the acyl chains against twisting. The dihedral distribution for the sat. chains show a peak-maxima at 180 o and two symmetric minor peaks at 60 o . We have observed that the anti-geometry (180 o ) is more favorable than gauche ) and the later follows a reverse trend in peak distribution with reducing temperature. Hence, we can infer, the bending of the sat. chains are more probable at higher temperatures indicating the fluid phase of the membrane bilayer. Nonetheless, the temperature dependence of the peak height is more visible for the sat. chains compare to cyp.