The two sets of fabricated ZrNT were subjected to drug loading and release studies, by monitoring the absorbance of Diclofenac sodium at 224 nm via UV-Visible spectrometry. Diclofenac sodium is a nonsteroidal anti-inflammatory drug and is used in this study as a model drug . For ease of understanding, the following simplification is made; homogeneously thick unmodulated ZrNT (13 µm tube length and 80 nm ± 20 nm diameter) will be identified as H -ZrNT, while the two variations of the modulated thick structures will be referred to as, M 1 -ZrNT : (1 st layer = 2 µm; 30 nm ± 5 nm diameter, 2 nd layer = 9 µm; 80 nm ± 20 nm diameter) and M 2 -ZrNT: (1 st layer = 9 µm 30 nm ± 5 nm diameter, 2 nd layer = 2 µm 80 nm ± 20 nm diameter. To confirm that the drugs were loaded into the entire length of the tube chemical imaging along the depth of the nanotube was done using a combined technique of FIB and ToF -SIMS. The FIB was used to create an incline revealing the nanostruc- interpore distance is higher in H -ZrNT; this can possibly translate to more drug molecules adhering at the surface rather than into the tubes, hence can be easily washed off during the washing step.