Electrophoresis DNA agarose gels were used to quantify the degradation of in situ self-assembled DNA origamis, in the presence of HEK cells. We identified DNA bands corresponding to well-formed origamis and free staples in cell media, and assumed that the DNA detected between these two bands corresponded to degraded origamis (Fig. ). Using Image J, we defined and plotted the lanes integrating the band of well-formed origami and the one attributed to degraded origamis, for each incubation time (Fig. ). The percentage of full DNA origami in the cell medium was calculated from the amounts of well-formed origamis and degraded origamis determined by peak area integration of fluorescence intensity.