Another validation we carry out is the ability of lipids to self-assemble. This was already investigated in a previous paper, but it is an important test to replicate here, since the optimized parameters are strikingly different compared to the ones used previously. In Finally, we test the phase separation of TG molecules in a DOPC bilayer, that was previously investigated by molecular dynamics simulations and experiments. We use TG here because the mapping does not introduce any new bead type, so we can also verify the transferability of the optimization on molecules that were not included in the training dataset. In our simulation, we use a concentration of TG that is above the reported critical aggregation concentration of 4% 68 , therefore we expect to observe phase separation from DOPC. In Fig. we report snapshots from the initial configuration and after 30 ns. We are indeed able to observe the formation of a TG blister, with a diameter of ∼18 nm and a height of ∼5.7 nm, inside the DOPC membrane. In reality, in our simulation the process of aggregation of the TG molecules starts much earlier, around the 10 ns mark, which is anyway consistent with the previously reported blister formation time of ∼25 ns. 58 Overall, the parameters optimized for the whole set of lipids (Table in the Supporting Information) perform just slightly worse than the parameters optimized for each lipid individually (Tables to S14 in the Supporting Information). The data for the losses, density profiles, area per lipid, and lateral pressure profiles for the individual lipids optimization are also shown in Fig. to S8 in the Supporting Information. Even though the APL and density profiles for the individually optimized lipids are closer to their experimental references, the parameters were also more prone to overfitting, leading to unstable simulations in some cases. In many cases, the parameters were also very different, so obtaining a single set of transferable parameters from this data is not trivial. Training a common set of parameters for all the lipids with the protocol presented in this work, provided transferable parameters which still lead to properties in very good agreement with the target values.