Subsequent results (figure ) validated this assumption by revealing a rise in TO fluorescence upon the incorporation of artificial saliva into the TO-cortisol aptamer complex. Despite the relatively notable standard deviations observed in the data points, signifying considerable variability among the five replicates, an R 2 value of 0.651 denotes a discernible correlation between the increase in fluorescence intensity and elevated hydrocortisone concentrations. Notably, the maximum percentage increase recorded was 31.77% for the highest TO concentration (5 µM).