Transcytosis across a bEnd.3 monolayer. Mouse brain endothelial cells (bEnd.3) were seeded at 1x10 5 cells/well in the apical compartment of a 6.5mm, 0.4m pore, polyester membrane transwell plate (Corning, 3470). Media was replaced daily and transendothelial electrical resistance was monitored using the EVOM 2 every other day. where C is the initial concentration at 0 min for the treated compartment. Pno monolayer is calculated using the Papp equation for a cell-free system. .