Cupric ions (Cu 2+ ) is the pivotal trace metal present in the human body and is indispensable for various physiological and pathological processes including facilitation of enzymatic processes, DNA replication, cell differentiation and protection against oxidative stresses. Anomalous imbalance of cellular Cu 2+ levels in human body resulting in number of pathological conditions related to renal diseases, cancer and neurodegenerative conditions like Alzheimer´s, Parkinson´s and Wilson´s diseases. Moreover, Cu 2+ is also a crucial metal pollutant in water and soil environment which could lead to the gradual accumulation in the plants and animals and ultimately to the humans. Thus, taking into consideration the adverse effects of aberrant concentrations of Cu 2+ ions, number of analytical methods have been used for its identification and estimation including electrochemical processes , atomic absorption spectroscopy , inorganic mass spectrometry , inductively coupled plasma spectroscopy , surface-enhanced Raman spectroscopy , and immune-chromatographic assay , but mostly these methods demand highly qualified professionals and instrumentation thus becomes very expensive and time-consuming.