By iterating through all pairs we calculate the distance of the pair and count the frequency of the distances. When considering all the possible isomers, the histogram of FG-FG distance frequency will have larger y-values but the same distribution, with the scale of: 1 ∶ 0.0625 ⋅ 4𝑛 linker . The reason for the same distribution is because each FG position has the same occurrence in the isomer enumeration. In one isomer, the probability of occurrence of every FG position on one linker is 0.25. Therefore, the occurrence of an FG-FG pair is 0.25 × 0.25 which equals 0.0625.