Polyanions' effect on TSLP-mediated TH2-polarization of CD4 + T cells TSLP is known to prime CD4 + T cells towards type 2 T helper cells (TH2) characterized by increased IL-4 and IL-13 release. After interaction of TSLP with its receptor (TSLPR), the resulting complex recruits the IL-7 receptor chain α. This recruitment leads in turn to phosphorylation of transcription factors, mainly STAT6 in CD4 + lymphocytes, which in turn promotes the expression of IL-4 and IL-13 (Figure ). We therefore used a CD4 + T cell assay to investigate the IL-13 release and thereby determine whether the strong electrostatic interactions influence the TSLP-mediated type 2 polarization of CD4 + T cells. (Before doing so, we used a cell viability assay (CCK8) to confirm that the investigated polyanions have no toxic effect on the T cells.)