We have performed an exhaustive analysis of KstD sequences based on multiple sequence alignment. We selected 83 sequences from a diverse set of organisms from over 5000 retrieved sequences that exhibited >90% query cover and >30% identity with respect to KstD1 (WP_020909157) from R. erythropolis SQ1, AcmB (WP_154715887), and AcmB2 (WP_067169324) from S. denitrificans (see Supplementary Information). The phylogenetic analysis showed that the analysed sequences can be divided into five general clades, which we named after representative KstDs with characterized substrate specificity such as ReKstD1-like from R. erythropolis, PsKstD3-like, PsKstD4-like from Pimelobacter simplex, and finally AcmB-like and AcmB2-like from S. denitrificans (Figure , Table ).