For the DPPC self-assembly validation, we started from the big simulation box system and dispersed the lipids by running a simulation at high temperature, 500 K. Then, to obtain the self-assembled membrane, we ran an NVT simulation at 323K. Finally, we investigated the phase separation of triolein, a triglyceride (TG), in a DOPC bilayer. The CG mapping of triolein was taken from Ref . We used one of the setups provided in Ref. , where the membrane is composed of 3200 DOPC molecules and 320 TGs. In this case, the box is approximately 33 × 33 × 17 nm 3 big, and contains ∼113 000 water beads.