To conclude, we demonstrate the importance of thermodynamic stability measurements in probing the rugged amyloid landscape and provide useful novel methodology for its analysis. We believe that future studies, that link thermodynamic stability of fibril polymorphs with high resolution structural information can provide a missing link between the observed structural polymorphism of amyloid fibrils and the properties relevant for disease. αSyn was precipitated by addition of saturated (NH 4 ) 2 SO 4 (4 mL per 1 mL of supernatant). The solution was incubated on a rocking platform at 4 °C for 15 min and then centrifuged (20,000 × g, 20 min, 4°C)