Step 1 uses Ligand GA. Then by using a cutoff of 79 for each of the non-isomeric molecules, 76 molecules are used in step 2. In step 2, these 76 molecules are processed with a more extensive set of parameters, -30 repeated docking calculations per ligand -pop_size=75 -n_islands=10 -max_ops=300000 -early_termination=0 (off)