The lipids were modeled with the CHARMM General Force Field (CGenFF) with the CHARMM compatible TIP3P water model. The topologies and CGenFF parameters were obtained using the CHARMM-GUI ligand reader and modeler. Generally, CGenFF is optimized to model small molecular drugs, and CHARMM36 31 is used in lipid membrane simulations. Therefore, the atom types, partial charges and dihedral potential functions were compared to the corresponding parameters found in the analogous lipid structures in CHARMM36. The CGenFF parameters were not changed, because these parameters deviated only slightly from those produced by CHARMM36, and since the CGenFF penalties were low.