PNIPAM folded above LCST and should decrease the overall size of PSiFe. This was visible in our experiments on thin films, where isotherms were shifted towards larger surface pressures at LT (Figure ) and upon transfer onto solid substrates, where distances between particles were higher at LT compared to HT (Figure ). However, we observed the opposite effect, i.e., increased hydrodynamic radii of PSiFe with increased temperature. The changes were relatively small but consistent. Our recent paper showed that for other batches of PSiFe, the change was from around 90 nm (LT) to 100 nm (HT). Similar results were reported for PNIPAM-decorated silica particles of comparable size in pure water. Mansfield et al.