Synthesis of fourth sugar ester-based mangiferin derivative, using raw mangiferin with (E)-3-(4-hydroxy-3-methoxyphenyl)acrylic acid and DMSO in the presence of immobilized lipase lipozyme in a water bath with thermostat control vibration shaking table at 60 °C for 24 hours, the reaction mixture was further concentrated with the help of a rotary evaporator that provided a residue that was subjected to purification with column chromatography using chloroform : methanol as eluent to provide the desired compound 4.