By considering that all streptavidin-ALP conjugates enter the nanogels and each NG consists of one DNA scaffold molecule, in our conditions (15 nM of strep-ALP for 1 μM of bt-DNA in phosphates) each nanogel can contain up to 100 loaded enzyme molecules. By assuming the nanogels to have spherical morphology and 130 nm diameter, the local concentration of the loaded protein can be evaluated to around 130 µM. High number of potentially loadable proteins can be useful for delivery into cells of multifunctional nanogels containing a large number of different proteins, and the high local concentration of the loaded proteins is promising for biomedical applications.