To study the capability of SOµC-Bdpy1 and SOµC-Bdpy2, as well as calculating the ratiometric signals, SOµC-Bdpy1 and SOµC-Bdpy2 were incubated in GSH solution (PBS, pH = 7.4, 37 °C) during different time intervals and GSH concentration. The selectivity experiments toward Cys and Hcy were also performed using Cys and Hcy solutions (1mM, PBS, pH = 7.4, 37 °C). Moreover, SOµC-Bdpy2 were incubated with 5 or 10mM of GSH during overnight and after remove the medium and SOµC-Bdpy2 were incubated for 4h with 5 or 10 mM of NEM, respectively.