Nanozymes are a huge group of nanomaterials such as carbon nanomaterials, metalbased nanoparticles, metal oxides, metal-organic frameworks, and nanoclusters that exhibit intrinsic enzyme-like activity . Among different nanozymes, most of them reveal significant peroxidase-like activity and cleavage of the peroxide bonds to produce active oxygen species such as hydroxyl radicals . The produced radicals can then react with chromogenic substrates and oxidize them to their corresponding colored products. The spectrophotometric assay and recording of the absorbance of these products can be used as an index for calculating the nanozymatic activity of the nanoscale peroxidase-like materials . Moreover, it is proved by several researches that the enzyme-like activity of the nanozymes can be inhibited by some inhibitors as same as the native enzymes. It is inhibitory effect can be used for several aims especially for sensing and detection toward developing both clinical and analytical protocols . It is well known that among different identified enzymes, peroxidase enzymes, especially horseradish peroxidase (HRP), are attractive enzymes from both industrial and clinical points of view . Regarding the peroxidase enzymes, hydrogen peroxide is the initiator of the peroxidase-mediated reactions and the oxidation of a wide range of organic compounds (substrates) including aromatic amines, phenols, and their mixtures can be initiated in the presence of hydrogen peroxide and peroxidase enzyme . However the peroxidase as same as other natural enzymes shows some of the following serious disadvantages such as pH and temperature instability, difficult recovery protocol, short storage time, no reusability, and highly expensive production methods. Hence, to fix these drawbacks, the immobilization of enzymes was proposed . However, during most immobilization protocols, the enzyme's initial activity is reduced and some of them are expensive. Hence, a better solution is needed to overcome these difficulties, the new field of nanozymes is the right solution . In fact, the fast development of nanoscience and material chemistry has -increased interest in researching new and innovative synthesis methods to produce new nanomaterials with unique catalytic activity , unique optical properties , high active area , antibacterial properties , and high biocompatibility . Among different nanomaterials, nanozymes as nanomaterials with high enzyme-like activity can be used to simulate enzymatic reactions in harsh environmental conditions (for example, higher temperature or wider pH range) .