Biodiesel has less global warming potential comparing to diesel. However, impact is seen to be increased in other environmental impact categories. Biodiesel (B100) would achieve reduction of 64.6% in GWP impact; however, AP, EP, and HTP impacts would increase by 31.6%, 61%, and 54.6%, correspondingly. GWP impacts reduction from B100 (~64.6%) calculated in this study are like as reported by Whitaker and Heath (2009) ~62% and Achten et al. (2010) ~55+16%. Except for GWP, it is observed that use of biodiesel will increase the life cycle environmental impacts. Though biodiesel is cleaner fuel while considering combustion phase only; but use of chemicals, fertilizers, fossil fuels, electricity in its early stages of life cycle results in increased AP, EP, and HTP. Also, biodiesel has higher NOx emissions during combustion phase.