The development of purely organic phosphorescent materials has garnered significant attention in scientific research due to their ability to exhibit high internal quantum efficiency, long exciton lifetime, and multiple stimulus response. Despite its merits, molecular thermal motion can quench phosphorescence from the triplet states, limiting conventional purely organic compounds to only exhibit phosphorescence at cryogenic temperatures. To overcome this limitation, several different approaches have been explored to enhance intersystem crossing (ISC) to realize room temperature phosphorescence (RTP) emission in pure organic materials, such as molecular aggregation, incorporation of heavy atoms, utilization of sensitizers, or suppression nonradiative decay through crystallization, matrix rigidification, and supramolecular self-assembly. In contrast, phosphorescence of isolated RTP molecules in dissolved solution (particularly in aerated solution) is unlikely to be observed, because of the significant molecular free volume with strong intramolecular motion, and great probability of diffusional collisions with quenchers (primarily oxygen). This can be attributed to the following reasons (Fig. ): 1) isolated RTP molecules with weak intermolecular interactions, large singlet and triplet gap (ΔEST), and inefficient ISC and radiative decay hinder phosphorescence emission; 2) conventional RTP molecules typically exhibit a low triplet level, which undergoes strong non-radiative processes in accordance with the energy gap law; 3) most of them lack the ability to shield the chromophores from external quenchers, contributing to easy quenching of phosphorescence. Therefore, these non-emissive RTP properties in solution make RTP materials difficult to meet the needs of optoelectronic devices, information anti-counterfeiting, biomedical imaging, phosphorescence probes, biosensing and other fields.