Finally, the high tunability of the lipid polar heads was exploited to functionalize the LipoGold tags surface with specific antibodies to impart targeting properties to the adducts. Validated through Fluorescence Correlation Spectroscopy (FCS) and a tailored sandwich assay, this approach's efficacy is confirmed in an in vitro setting, where the SERS signal and specificity are retained within cell cultures. Overall, this work proposes a very straightforward and powerful method based on simple and rapid self-assembly to produce AuNPs-vesicle hybrid materials for diagnostic and imaging purposes, addressing current challenges in the fabrication of metallic nanoarrays for SERS applications. with a narrow and reproducible size distribution. The extrusion was performed with the Extruder (Lipex Biomembranes, Vancouver, Canada) through Nuclepore membranes.