Based on the two-channel set up, an on-sight defect reaction was performed. Around 0.25 nM purified (6,5)-SWCNT solution (1% SDBS) was incubated on a glass bottom petri dish for 2 hours to yield single SWCNTs on glass. Washing was performed as previously described. 200 µL 1% SDBS was added ontop of the immobilized SWCNTs, two camera recording was started with the laser on and 2 µL diazonium salt was added to monitor the intensity change of single SWCNTs over time in both channels.