In summary, we developed a method based on the pseudo-infection model to visualize the transfer and distribution of viral macromolecular contents to target vesicles. Until now, this model has been utilized to characterize the earliest stages of viral infection, such as, binding of virus particles to membrane-bound receptors, and mixing of viral and vesicular lipid compartments and contents. Moreover, from previous content mixing assays based on dequenching of water-soluble dyes, it is not possible to infer whether the viral contents themselves completely mixed within the fused volume. Also, in a previous genome exposure assay , the issue of distribution of viral contents within the fused volume was not addressed. Here we show that the utility of pseudoinfection model can be expanded to probe the distribution of viral contents within the fused volume. Whereas in the past the question of viral content distribution has been addressed using advanced structural techniques like cryo-ET, we showed that relatively simpler fluorescence microscopy-based measurements can be used to derive such spatial information.