Hormones can be measured in different matrices like blood, saliva and urine. A good understanding of a person's hormone levels can give an indication of health. Blood has been the gold standard for measuring various hormones . However, saliva is a better matrix and can be collected with non-invasive methods . Currently, HPLC (High Pressure Liquid Chromatography) coupled with mass spectromtery and ELISA (enzyme linked immuno-sorbant assay) are the most common ways for measuring the quantity of these metabolites . HPLC can be multiplexed for determination of steroid hormones and it is easy to interpret the data. One drawback of HPLC is that it is centralised and can have relatively high startup costs . ELISA plates have less startup costs and only a simple absorbance plate reader is needed. However, the running costs of these plates can be relatively expensive as each plate can potentially cost hundreds of dollars. One potentially cheaper alternative to antibodies is to use chemical antibodies or aptamers to bind to the small molecules on a 96-well plate .