To confirm that the observed CO2 release was indeed due to the photoacid effect, control experiments were conducted. In the first control experiment, the photoacid was omitted while keeping all other conditions the same. Even in the absence of the photoacid, a CO2 release of 0.039 mmol was obtained within 30 minutes. This result was attributed to an increase in temperature caused by the irradiation, leading to reduced CO2 solubility at higher temperatures. However, the temperature increase (35 °C) was lower than under the standard conditions due to the absence of a photoactive molecule. The temperature reached approximately 60 °C within 30 minutes under the standard conditions due to the photothermal effect of pyranine. In the second control experiment, thermal heating was applied to induce CO2 release at 60 °C. A standard solution containing 100 mM of pyranine and 100 mM of bicarbonate, saturated with 15% CO2, was heated using a hot plate within an oil bath. The CO2 output measured was 0.072 mmol within 15 minutes. These results indicated that a combination of the photoacid effect and the photothermal effect contributed to the CO2 release. Specifically, the photoacid effect accounted for the release of 0.058 mmol of CO2 under the standard conditions, while the remaining 0.072 mmol was attributed to the photothermal effect.