The definition of exclusion correction raises an important question, that is how to determine ε 𝑖𝑗 𝑒𝑥𝑐 for all CG-CG and CG-AA interactions by a physical-based and consistent approach. The appending the exclusion term shifts the distance zero point 𝑟 𝑖𝑗 0 of the potential, from 2 -1 6 σ 𝑖𝑗 to 2 -1 6 𝜎 𝑖𝑗 𝛾 . It is not difficult to understand that the probability of finding 𝑟 𝑖𝑗 < 𝑟 𝑖𝑗 0 in an MD simulation is very low because the LJ potential is sharply raised in this region. Thus, the region encircled by LJ zero-point potential surface can be understood as a type of "volume". Appending the exclusion term yields the changing (usually extension) of this "volume". Therefore, we define a property called effective volume to measure the strength of LJ exclusion, which is defined as the volume encircled by the zero-point potential surface. It should be noted that the effective volume is related to the LJ parameters of the observer (probe). e.g.