After purification, the loaded liposomes were diluted to obtain a target peptide concentration between 50 -175 μg/mL. The diluted liposome formulation (1.0 -1.5 mL) was filled into 100 kDa Float-A-Lysers and were dialysed at different temperatures (room temperature, 34 °C, and 45 °C). At pre-defined time points 50 μL of each liposome formulation was withdrawn and quantified for remaining peptide concentration. The percentage of peptide released at every time point was calculated as described in Equation 1: