Note that water-immiscible solvents such as chloroform cannot be used for the post-insertion of anionophores. -Strangely shaped transport curves can be obtained if the mixing of the NaCl solution with the liposomes is not optimal, because the stirring bar is absent, the stirring bar is spinning too slow or too fast, or if the added NaCl solution has ended up on the edge of the cuvette rather than in the liposome suspension. An alternative cause for strangely shaped curves can be the slow incorporation of a transporter. In this case, it is recommended to wait at least 3-5 minutes between the addition of the transporter and the addition of NaCl.