This result was also consistent with the literature that glycoconjugates of larger sizes and higher glycan valency generally offered higher viral inhibition potencies. (4) G27-DiMan (100%) exhibited the strongest DC-SIGN affinity among all Gx-DiMan conjugates studied here, with an apparent Kd of ~0.2 nM, its affinity was ~29 and ~5 folds stronger than its G5-and G13-DiMan(100%) counterparts.