The TLC plates used in this study were made of silica gel F254 precoated with aluminum plates and were of 0.25 mm thickness procured from Merk, Germany. The developed plates were first visualized in a U.V lamp chamber at 254 and 365 nm. The TLC of crude extract was analyzed in various solvent systems to identify the appropriate solvent for separation of pure compounds in column chromatography. TLC profile of chloroform/methanol (1:1) and methanol crude extract were conducted in n-hexane/ethyl acetate, dichloromethane/chloroform, ethyl acetate/ethanol, and n-hexane/dichloromethane/ethyl acetate combination by varying the combining ratio. The nhexane/ethyl acetate combination showed relatively good separation on chloroform/methanol (1:1) crude extract, but methanol extract didn't show separate spot. Thus n-hexane/ethyl acetate was a suitable solvent system and chloroform/methanol (1:1) crude extract have good TLC profile were selected for column chromatography.