Among all the tested peptides, 8a and 8c (composed of all D-amino acids) demonstrated high hemolytic activity comparable to parent peptide p1 and showed the highest flexibility (RMSD values of 1.2 and 1.4 Å, respectively). In contrast, the least toxic lead peptides, 15c and 16c, showed slightly lower conformational mobility during MD compared to their parent analog, p3 (Table , Supporting Information). Overall, these findings point towards a complex relation between conformational mobility and hemolytic activity in the tested cyclic peptides, and therefore, a more detailed conformational analysis is required.