Gold-aptamer-nanoconstructs sensitive for to a target enteroviral nucleic acid sequence, conserved amongst 96% of all known enteroviruses were fabricated. Aptamers were designed in silico to fully complement the target enteroviral nucleic acid and partially hybridise with gold nanoparticles functionalised with complementary nucleic acid. In silico design provides a flexible platform that can be readily optimised to produce multiplexed devices capable of simultaneously detecting an array of analytes. For example, gold-aptamernanoconstructs could be multiplexed to simultaneously stratify patients presenting with symptoms of viral, bacterial and mixed viral/ bacterial infections. In the presence of the conserved target enteroviral nucleic acid sequence, a signal transduction mechanism was initiated and gold-aptamer-nanoconstructs disaggregated. Colourimetric (purple to red), spectroscopic (544nm to 524nm) and lateral flow assays (binding of biotin functionalised gold nanoparticles to streptavidin doped membranes) were shown to be sensitive towards the target nucleic acid sequence ≥ 1x10 -7 M (≥1.4×10 -14 g/mL). Lateral flow assays were unaffected by contaminating human DNA and produced a result in less than 60 s. We anticipate our assay, that is economical, simple, rapid assay could be translated into a desktop detector device through optimisation of sample preparation, improvements in detection limit and clinical evaluation, for viruses and related organisms, that is accessible at the point-of-care, could pave the way forward in the detection of enteroviruses and complement existing strategies aimed at overcoming antimicrobial resistance.