At the core of the CACHE initiative will be an experimental hub (or hubs) that will provide rapid, highquality testing of the predictions. Predicted compounds will be submitted to the experimental hub, which will procure the compounds and assay them using a binding assay selected to be most appropriate for the protein target. Each compound will be assayed at a single concentration in duplicate, and each positive re-tested in dose-response mode, as well as in an orthogonal biophysical assay, which is critical. Feedback will be given first to the predictor(s), who will have the opportunity to submit a new set of predictions.