Here we studied the effect of a protein corona on the interplay between SNPs of two different sizes and DOPC giant unilamellar vesicles (GUVs). To mimic the protein coverage experienced by NPs in biological media, we incubated the SNPs with bovine serum albumin (BSA) proteins, a major constituent of blood plasma and cell culture media. This leads to a simplified protein corona made exclusively of BSA, which provides information about changes in non-specific interactions between both types of SNPs and the lipid membranes induced by the surface coating of the NPs with proteins. We prepared initial stocks of 10 mg/ml SNPs suspended in a solution of 100 mg/ml BSA in the experimental buffer (20 mM HEPES, 150 mM NaCl, pH 7.4). The concentration of BSA (100 mg/ml) used to prepare the BSA-coated SNPs (SNPs@BSA) is comparable to the total concentration of proteins in blood serum. The stock solutions were incubated at 4 ⁰C for 24 h (unless otherwise specified) and then diluted into experimental samples at the desired concentrations.