To explore how the rotational flexibility of ferrocene could impact the dynamic conformational changes of those compounds, we performed proton nuclear magnetic resonance (¹H-NMR) studies to gain more structural insights into FerroTACs in solution. For NMR studies of highly flexible molecules like PROTACs, linkers` chemical properties and flexibility are crucial in shaping their behaviour in solution. Such molecules generally exist as conformational ensembles, featuring diverse arrays of distinct conformers stabilised by weak intramolecular interactions. The dynamic flexibility of the linker allows for rapid interconversion of these conformers on the NMR experiments` timescale, leading to the observation of only an averaged NMR spectrum. With this premise, we performed NMR studies to quantify the HBD solvent exposure of the amide protons using the HBD Acidity NMR (ANMR) score and variable temperature studies (vt-NMR) . Both techniques are well-established methods for studying the intramolecular hydrogen bonding (IMHB) patterns in PROTACs as well as in ferrocene dipeptides. ¹H-NMR spectroscopy experiments were conducted to investigate the intramolecular dynamics of FerroTAC AS4 and its closest Fc-free linear analogue, 14a. AS4 was chosen for the conformational study because i) it bears two different substitutes (CRBN and VHL ligands) and ii) its two amide groups (N(3)-H and N(4)-H) are positioned near the ferrocene moiety, which facilitates monitoring of conformational changes compared to other FerroTACs in our library containing a 1,2,3-triazole group or the symmetric homo-FerrTACs.