WT-metaD simulations were run with the metadynamics class of OpenMM 7.5. The simulations were run at 298.15 K employing a Langevin thermostat with a damping coefficient of 1 ps -1 . Hydrogen mass repartitioning was used, with the mass of each hydrogen atom set to 1.5 amu. The integration timestep was 4 fs. It was also deemed necessary to introduce additional restraints on the ϕ, ψ torsions of coiled-coil monomers to further preserve their helicity. Three protocols were considered in this study. Additional details about the simulation protocols are given in the SI (SI Sections 4 and 5).