Lateral flow assays (LFAs) have limitations in their sensitivity and detection limits, which restrict their applicability for a broader range of analytes. To address this issue, a team of researchers proposed a simple and easy-to-implement approach for improving the sensitivity of LFAs by introducing delay hydrophobic barriers fabricated by wax printing. The wax-printed patterns, consisting of several wax pillars, were deposited on the nitrocellulose membrane to create delays and pseudoturbulence in the microcapillary flow. Mathematical simulations were carried out to corroborate the experimental results obtained for the different patterns tested for the detection of HIgG, a model protein, in a gold nanoparticlebased LFA. The results showed that the proposed wax pillar-modified devices significantly improved sensitivity, with a nearly 3-fold increase compared to conventional free-barrier LFAs. The barriers delayed the regular flow on the strip, thereby increasing the binding time between the analyte and the labeled antibody and facilitating effective formation of the immunocomplex. The optimized designs exhibited improved sensitivity, as demonstrated by mathematical simulations. This approach is simpler than other previously reported strategies, as it is low-cost, not time-consuming, and does not require additional reagents for signal amplification or changes in the configuration of the LF strip. As such, it can be easily extended to any type of LFA design and could expand the scope for the use of LF designs with patterning techniques, facilitating their use in point-of-care applications. Overall, this proposed strategy has the potential to broaden the application of LFAs in detecting various analytes with high sensitivity and low detection limits.