To normalize measurements, background fluorescence signals of the assay solution without vector was subtracted from each emission scan. Inhibition is reported relative to the control reaction with vector and DMSO. Two compounds, 11a and 11b, showed some inhibition in an earlier version of the cell-free transcription/translation assay where DHFR production was monitored by PAGE (data not shown). In the fluorescence assay, compound 11c demonstrated weak inhibition (Fig. ), reducing the EmGFP signal by about one-third (35%). Erythromycin, included as a positive control, reduced fluorescence by 99% at 1 μg/mL (Fig. ), suggesting almost complete inhibition of EmGFP synthesis.