Stem cell therapies are widely used in the regenerative medicine thanks to their intrinsic biological characteristics, such as plasticity, self-renewal, and multiway differentiation ability. Stem cell treatment includes human autograft or allograft cultured stem cells locally injected into specific parts of the body or administered by intravenous infusion. Thus, bringing active stem cells into the body can rejuvenate existing cells and allow the body to age more gently and even reverse some impacts of the aging. Currently, neural stem cells, bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells, adipose stem cells, embryonic stem cells, and human induced pluripotent stem cells are the most closely related anti-aging agents and exhibit direct (cellular replacement) and indirect (paracrine) effects. Pluripotent stem cells naturally differentiate when culture conditions no longer support their pluripotency. Thus, pluripotent stem cells can be directed towards preferred cell identities when specific stimuli are supplemented, such as those available during embryonic differentiation. There are many examples of pluripotent stem cells differentiation. The differentiation of pluripotent stem cells into renal podocytes, hematopoietic progenitor cells, neurons, endothelial cells, cardiac muscle cells, retinal progenitor cells, pancreatic β islet cells or ciliated epithelial cells, infers no limits to human tissue modeling in vitro. The reported progresses in organoids development also demonstrates the advanced knowledge in cell manipulations. Three-dimensional cultures of pluripotent cells let them organize and differentiate into spheroid structures, with several cohabitant cell types. The most sophisticated current organoid models relate to brain, intestine, kidney, heart, or retina. With the emergence of cell 3D-printing technologies using pluripotent stem cells or differentiated cells as inks, impressive progress has been successful in the formation of heterogeneous tissues. The development of ear cartilage regeneration utilizes this technology. Thus, stem cells may be capable to defer aging in several ways: