We manually searched the PubChem BioAssays database using terms such as 'high-throughput screening', 'primary', 'confirmatory', 'single dose', 'dose response', etc. The manual search step is necessary due to the nature of reporting HTS results. Primary and confirmatory screening results might be reported in the same bioassay, e.g. AID 1445 (AID, assay identifier), or as separate bioassays that are part of the same project, e.g. primary AID 602261 and confirmatory AID 624326. Furthermore, different primary and confirmatory projects use different formats and conventions when reporting measurements. Among the selected bioassays, primary screens might be reported at different concentrations and with multiple replicates. Similarly, confirmatory screens can be reported in different formats (usually IC50, AC50, or EC50), but the units (e.g. uM or ug/mL) are not always consistent. The smaller collection of 23 datasets previously discussed by Buterez et al. focused on a diverse, but restricted number of assays to allow for an in-depth analysis of both PubChem and proprietary data, while also emphasising certain dataset attributes such as the correlation between the SD and DR values. The updated collection presented in this work further increases the diversity of the assays and the number of curated datasets, allowing a more comprehensive view of HTS modelling strategies and challenging current methods.