3). To isolate PLY-induced pores, in the mixture of liposome (2 mM), PLY (1mg/ml) and Cymal-6 (0.56 mM) after their respective incubation time, amphipol was added in a ratio (w/w) 5:1 to PLY. This mixture was incubated for 30 min at room temperature. Before applying the sample to Cryo-TEM grid, detergents were exchanged with PBS by dialysis cassette (Slide-A-Lyzer® G2, Thermo scientific, USA). Buffer was exchanged for 2 h and again 2h, then overnight at 4 ºC.