A considerable challenge when deciphering the exact morphology of copolymer assemblies is the structural ambiguity that is provided by certain routine characterisation techniques such as conventional dynamic light scattering (DLS) and negative stain transmission electron microscopy (TEM). However, accurate information on morphology is of great importance since it directly affects choice of suitable disease targets, drug cargo, delivery route, stability and release mechanism. Thus, the use of additional complementary characterisation techniques is needed to characterise the morphology of soft self-assembled nanostructures.