The burst pressure test is a critical method used to evaluate the mechanical strength and integrity of vascular scaffolds. It determines the maximum pressure the scaffold can withstand before rupturing, providing crucial information on its performance under physiological conditions. To conduct the burst pressure test, a specially designed home-made setup was utilized (figure ). The setup consisted of several key components, including a Syringe Pump (Ascor Single-syringe pump AP14), a syringe, and a pressure gauge. The vascular scaffold was connected to a closed tubular system at one end, while the other end was attached to the pressure gauge. To simulate physiological conditions, the syringe was filled with phosphate buffered saline (PBS) at a temperature of 37°C. The burst pressure test was initiated by starting the injection, and the flow pressure inside the vascular scaffold was carefully observed. During the test, a constant flow rate of 50 mL/min was applied to fill the engineered vascular scaffold with the PBS buffer. The flow rate was precisely controlled using the syringe pump, ensuring consistent conditions for all tested scaffold samples. As the scaffold was filled with the PBS buffer, the pressure inside the tubular scaffold gradually increased until it reached a peak value just before bursting. Figure represents the picture of the homemade setup for burst test including the picture of the scaffold before and after bursting. This peak pressure was accurately recorded using the pressure gauge and considered as the burst pressure for the scaffold. To assess the effect of gelatin coating on the burst pressure, the test was conducted on graft samples with and without gelatin coating. This comparison aimed to understand how the coating may influence the mechanical strength and performance of the vascular grafts. For each group, three samples were tested to provide reliable and statistically significant data.