From the morphological viewpoint, when exposed to IONFs-C1, PLTs generally showed a more elongated shape when compared to the control and IONFs-C3 samples from the same blood sample and for the same incubation time. As shown in Figure , at higher magnification the typical flower-shape IONFs could be observed and were abundant in the extracellular environment. After 30 minutes incubation with IONFs-C3, no major IONF aggregation was detectable in extracellular or intracellular environments, where an abundance of particulate matter (50-60 nm in size) related to glycogen was found. Only a few IONFs could be identified (as indicated by the turquoise arrow). Finally, vesicular formation referable to ectosomes could be visualized in the case of IONFs-C1, and in lower amount, of IONFs-C3 and control (indicated by pink circles). To conclude, TEM images confirmed the biological data as well as the hypothesis that IONFs-C1 concentration can induce notable alterations to PLTs, while undergoing clustering, membrane interaction and internalization.