Commercial, non-toxic PCL (Capa™ 6430) were used as negative control, nitrile glove (Mercator Nitrylex Classic, Kraków, Poland) was used as positive control, and media in empty well as sham. After 24 h, the cell culture medium was aspirated from the plate and replaced with 100 μL of extract medium from a given sample (5 technical repetition: 3 samples per material). The plates were then returned to the cell culture incubator for another 24 h of culture. 20 μL of resazurin with a concentration of 0.15 milligrams per milliliter in phosphate-buffered saline were added to each well, including the control wells containing just the media, and the samples were incubated for an additional 4 hours at 37 degrees Celsius. The fluorescence intensity was then determined using a Biotek Synergy HTX multi-functional plate reader (Biotek, Winooski, Vermont, USA), with a 540 nm excitation wavelength and a 590 nm emission wavelength.