A Q Exactive HF quadrupole-Orbitrap mass spectrometer coupled to a Vanquish UHPLC system (both Thermo Fisher Scientific) via heated electrospray ionization was used for the high-resolution measurements. To ensure maximal comparability, the column, eluents, gradient, injection volume and other LC parameters were identical to the low-resolution (QQQ) measurements. The settings of the ESI interface were as follows: sheath gas, 45 au; auxiliary gas, 15 au; sweep gas flow, 2 au; capillary voltage, 3500 kV (positive), 2800 kV (negative); capillary temperature, 300 °C; auxiliary gas heater, 350 °C.An MS full scan from 60-900 m/z was acquired, and, consistent with the QQQ measurements, polarity-switching mode was activated. Automatic gain control (AGC) was 1 x 10 6 and the resolution was 60,000 (@ m/z 200). The maximum injection time was 100 ms. For both approaches, each concentration in pure solvent and urine was injected at least twice within the sample sequence. Selected concentrations (n=4; levels 300/30/3/0.3 ng/mL; see Table ) were injected 10 and 11 times for the solvent and urine, respectively. MS2 fragmentation was not performed. The identical sequence order was measured on both instruments to maximize the alignment of experiments.