In addition to the experiments with DPPC or higher cholesterol concentrations, the lucigenin assay can also be used with DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine) and analogous lipids of different lengths. This allows to study the impact of the diffusion on the transport process. Furthermore, anionic lipids can be used, such as POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol)) or POPS