The equilibrated CG-NM membrane (193 lipids per leaflet) at 310 K is obtained from the website: . coli-PLE. After that it is multiplied four times using GROMACS. We have considered equal number of lipids in each leaflet. Henceforth, each leaflet contains 772 lipids. We have solvated the membrane with 38600 CG-water beads which is equivalent to 100 water molecules per lipid to keep the membrane fully hydrated. We have also added 480 Na ions (counter ions) for charge neutralisation. This is a reasonably larger system size to study the structure and phase behaviour of a complex multicomponent membrane system. To prevent the freezing of Martini CG-water at lower temperatures, 10% CG-water beads are replaced with antifreeze particles . These antifreeze -particles does not interfere with the structure, fluidity, or phase behaviour of the lipid bilayer. The initial structure for MMS and MMU systems are taken from NM system and the forcefield for lipids are modified accordingly for the former two membranes. The martini bead containing cyclopropane ring is parameterised with the same bead type (C1) as for the saturated bead consistent with the literature . To maintain the compatibility and universality, the bond lengths are kept unchanged: 0.47 nm for bonds within the hydrophobic tail and 0.51 nm for the bonds connecting glycerol and tail. But the angle containing cyclopropane ring in the middle is reparametrized to 125° with a harmonic constant of 15 kJ/mol/nm 2 . For the MMS and MMU systems we have replaced the forcefield for CP containing lipids with its analogues already available in martini website. For those which are not available, we have changed the angle from 125° to 180° and 125° to 120° for the single bond and double bond containing lipids in the middle respectively. In addition, the angle force constant is set to 35 kJ/mol/nm 2 for the corresponding single-or double bonds in MMS and MMU systems. C4 bead type is chosen for double bond containing beads.