This degrader, 3B-1, consisted of ONC201 linked to a POLRMT inhibitor. However, 3B-1 required 16 hours at a concentration of 40 μM to induce degradation of POLRMT, whereas our degraders worked within only 2-4 hours, and the DC50 values were 4.8 μM (HeLa) and 0.96 μM (MCF7), respectively. This may be attributed to the weaker ClpP-activation potency of ONC201 as compared with TR79 31 , though a difference in stability between POLRMT and mSA could also have contributed to the outcome. Interestingly, Wang et al. found that increasing the length of the ethylene glycol linker in 3B-1 resulted in loss of activity, whereas we found that a longer linker increased the degradation activity. Two possible explanations can be considered: 1) the potency of ClpP activators affects the potency of ClpPbased degraders, and 2) the optimal linker length varies depending on the target protein. The technology reported by Wang et al. and us differs from PROTACs in using degraders that directly recruit the protease complex. Therefore, various features, such as characteristics arising from ternary complex formation and degradation time course, are expected to differ from those of PROTACs. Further work will be needed to fully elucidate the characteristics of this technology.