In this work, a novel soft gold ultramicroelectrode was developed on the widely used soft carbon stylus microelectrode (Ametek, USA). The carbon paste part was replaced with 25 µm gold wire to enhance the stability of the probe. The problems of swelling and exfoliation of the carbon paste were solved. The 100 µm polyethylene terephthalate (PET) and Parylene C insulating layer were substituted with 5 µm PI film.