Overall, we demonstrate that the mpLFIA platform provides a highly sensitive and quantitative approach for infectious disease detection, offering a cost-effective solution for large-scale screening. Notably, it requires only a magnet to concentrate the nanoparticle system and a visual colorimetric probe: mpGNS-3, making it a practical and accessible tool for point-of-care diagnostics in low-resource settings. Its simplicity and efficiency make it particularly suited for early disease detection before hospital admission, helping to curb the spread of infections in densely populated areas.