Further, we tested the effect of cell matrix on peak tailing by spiking four representative acyl-CoA standards in HepG2 cells after extraction. The ammonium acetate concentration of the mobile phase was kept at 5 mM, and peak asymmetry factor was compared between acyl-CoA standards spiked in the cell samples and the standards that were spiked in samples not containing cell matrix. It was observed that peak tailing and subsequently asymmetry factor has been reduced due to the presence of cell matrix (Figure , Table ). One possible explanation behind the reduction of peak tailing in the presence of cell matrix is that the components within the cell samples can act as masking agents, thus occupying the tailing sites on the column surface. As a result, the interaction between the acyl-CoA molecules and the column is reduced, leading to a decrease in peak tailing during chromatographic analysis. However, additional investigations are required to confirm this hypothesis. This can also be valuable as understanding the role of the cell matrix in reducing peak tailing can provide valuable insights for optimizing analytical methods and can be helpful in developing strategies to minimize peak tailing and improve the overall performance of chromatographic analyses.