Simple western immunoassay: Fluorescent (5X) master mix, DTT and biotinylated ladder were prepared as per manufacturer's instruction (BioTechne). Lysates were diluted with 0.1x sample buffer and mixed 5:1 with fluorescent 5x master mix to obtain a target sample concentration of 0.40 µg/µl per well. Samples were then denatured for 5 min at 95°C in a PCR cycler (GeneAMP PCR System2700, Applied Biosystems). The assay plate was loaded as per manufacture's instruction and centrifuged for 5 min at 1000 x g at RT. The immunoassay was performed using a 12-230 kDA separation module with 25 cartridges (SM-W004, BioTechne). Lysates were separated 25 min at 375 volt, blocked for 5 min with antibody diluent 2, 30 min incubated with primary antibody and 30 min incubated with secondary antibody, subsequently. Primary antibody multiplex mix consisted of 1:100 anti-Hsp90 (#4877S, Cell Signaling Technology, Danvers, MA, USA) and 1:50 anti-β-actin (#MAB8929, R&D) diluted in Antibody Diluent 2. Signals were detected using an anti-rabbit detection module of JESS (DM-001, BioTechne), multiplexed with an anti-mouse secondary NIR antibody (#043-821, BioTechne).