It is important to note that phosphoenolpyruvate (PEP) is typically present in the cytoplasm, as glycolysis takes place in the cell's cytoplasmic matrix. Pyruvate kinase is responsible for the primary production of ATP in the cytoplasmic matrix. Due to the low amount of PEP in the nucleus, tau protein can't carry out the Aging Device function as a high-frequency physiological event, like glycolysis. Tau protein translocates to the cytoplasm, where it obstructs the glycolysis pathway and generates ATP through pyruvate kinase activity. It is highly likely that the spatial capacity constraints of nuclear translocation and mitochondrial transport will cause the tau protein to become obstructed and congested in the cytoplasm. Indeed, aberrant phosphorylation can disrupt the pyruvate kinase activity of tau protein, leading to its inability to synthesize ATP and its accumulation in the cytoplasm.