Cyclo-reversion of furan-maleimide cycloadduct under mechanical force has so far been only observed in case of single polymer chains. The mechanical stretching was provided using ultrasound. Proximal cycloadducts, such as we have in the system, had been demonstrated to undergo cyclo-reversion under mechanical stretching. In a recent study, researchers have demonstrated that such cyclo-reversion under tension may follow a pericyclic or a non-pericyclic pathway depending on the amount of applied force. Nonetheless, such cyclo-reversion induced by shear-stress has not been observed so far in ECM-polymer derived hydrogel. Plausible chemical structures involved in this process are depicted in Figure . Although, it appears to be intuitive that having mechanophores within crosslinks will result in a hydrogel to be self-healing, this is vastly oversimplification. Several studies in this field debate and discuss the difficulties in activating a mechanophore in a crosslinked hydrogel system where the applied macroscopic stress was never distributed homogeneously over the complete crosslinked network and then funneled to the mechanophores. In fact, only a tiny fraction of the mechanophores are usually activated through applied macroscopic stress. To our surprise, the shear-thinning and autonomous selfhealing behaviors of the hydrogels developed here indicate towards mechanophore activation using macroscopic stress. To the best of our knowledge this is the only example of hydrogel of this kind. Although intriguing, it should be noted that rheology is a macroscopic evaluation technique, and apparent complete macroscopic autonomous healing in terms of rate of gelation, moduli, or linear viscoelastic limit does not necessarily imply complete healing at a nanoscale. More studies, especially those which can reveal nanoscale structural information under stress, could be great tools to explore this further which would be instrumental in rationally designing such hydrogel systems. Nevertheless, ECM-derived injectable hydrogels with shear-thinning and autonomous self-healing, shape-fidelity, and non-swelling over long term is a major leap forward in the field of injectable biomaterials. In vitro biological characterization of hydrogels. To investigate the impact of crosslinking chemistry on cell viability, we conducted an in vitro assessment of cytocompatibility for r2 hydrogels. Human fetal cardiac mesenchymal stromal cells (hfcMSCs) and Human Umbilical Vein Endothelial Cell line (HUVECs) were employed as model cells, encapsulated within these hydrogels. We evaluated cell viability at both 1 day and extended it to 7 days post-encapsulation using the Calcein-AM/ethidium homodimer Live/Dead assay, examining the fate of both hfcMSCs (Figure ) and HUVECs (Figure ).