S. mansoni adult worms were recovered by hepatic portal vein perfusion from TO mice (Mus musculus HsdOLa:TO -Tuck Ordinary; Envigo, UK) that were percutaneously infected seven weeks earlier with 180 cercariae. Following perfusion, schistosomes were washed and processed as previously described. Subsequently, schistosome pairs were seeded into 48 well tissue culture plates (1 worm pair/well, in duplicate) and dosed with the compounds (20 μM in 0.2% DMSO).