Environmental conditions such as high pH and high temperature may destabilize AgNPs and ionize phenol into phenolate anions which may decrease the removal efficiency of phenol in waste water . AgNPs used in the removal of phenol and its derivatives when released into the environment have toxic effects on mammals, non-mammals and plants. The toxic effects on mammals were studied in an experiment in which Sprague-Dawley rats were exposed to AgNPs of size 18 nm of dose concentration 2.9X10 6 particles cm -3 . This experiment resulted in lung inflammation and damage to respiratory system . In another similar study conducted by Kim et al. , Sprague-Dawley rats were ingested with AgNPs of size 60 nm which are mixed with diet for 28 weeks, which resulted in accumulation of AgNPs in kidney. In an experiment demonstrating the toxic effects of AgNPs on non-mammals, Zebrafish embryos were exposed to AgNPs of size 11.6 nm ± 3.5 nm for 120 hours. This resulted in 100 percent mortality and malformation of embryonic morphology . A study on Lolium Multiflorum was conducted to evaluate the effects of AgNPs on plants in which L. multiflorum was exposed to AgNPs of size 6nm with concentration 1-4 mgL -1 for 7-21 days. This study reported the decrease in root and shoot length .