Triblock copolymer aqueous solutions, CUR or IBU loaded formulations were prepared with PBS (pH 7.4) and measured on Zetasizer Nano ZSP from Malvern, (Malvern Instruments, Worcestershire, UK) in disposable cuvettes (UV cuvettes semi micro, BRAND GmbH, Wertheim, Germany) at ambient temperatures (≈25 °C). Data was analysed by using Zetasizer software 7.11. All the samples were measured after filtration using 0.45 µm PVDF syringe filter (Rotilabo, Karlsruhe).