ViennaRNA 2.0 was used to model a 2D predicted secondary structure of the MinE07 aptamers, using the thermodynamic structure prediction and the RNAfold web server. The dot and bracket sequence was used to generate a PDB file to create 3D structure using RNA composer. The PDB file was opened in text editor and all the 2'OH atoms are deleted from uridine and cystine. This PDB can be visualised using the cross platform molecular builder and editor program, Avogadro (avogadro.cc). The geometry was then optimised using the optimise geometry option in Avogadro with force field MMFF94, which is in a vacuum. EGFR PBD file (1IVO) was taken from the Protein Data Bank. For docking studies the following programs were used Autodock Tools (MGLTools 1.5.7) available from: mgltools.scripps.edu/downloads, Autodock Vina 1.1.2 run via MPI Vina on a multi-node cluster and PyMOL 2.5.3 Schrödinger L, DeLano W. PyMOL 2020, available from: pymol.org/pymol. Blind docking was first undertaken, parameters: box position (X Y Z, Å) 83.72, 56.20, 50.18, size (Å) 126x126x126. Docking on binding site were then undertaken with the parameters: box position (X Y Z, Å) 93.00, 82.00, 72.00, size (Å) 61.24x45.68x44.70.