Centrifuge the solution at 2000 rpm for 2 minutes. Followed by the washing step where we discard the supernatant and add 10 ml of distilled water. Perform the washing step 5-6 times until the pH of the solution is neutral (pH 6.5). Make sure the sides of the centrifuge tubes are sealed with Aluminium foil to prevent light exposure as Thymoquinone is lightsensitive. Finally, discard the supernatant and transfer the pellet to a Petri plate and place it in a hot air oven set at 60 degrees Celsius for strictly 8 hours to avoid crystallization. Scrape out the particles using a sterilized spatula and store it in a 1.5ml sealed tube away from sunlight.