The application of Zeno trapping shows an approximate gain in sensitivity of five times, and about 100 analytes can be monitored in a single LC-MS run. Besides providing better signals for the product ion spectra, the information gained with EAD and UVPD can be used to confirm the general screening results or to provide additional structural information. All three modes are also suitable for quantitative analysis. The benefit of the additional activation methods is a different fragmentation selectivity towards co-eluting isobaric compounds or background signals for a given analyte. The MS response is analyte dependent, and the limit of detection (LOD) based on standard solutions was estimated to be in the range pg/ml to ng/ml based on an injection volume of 80 µL (S/N > 3). A more detailed evaluation of LOD for detected analysis presented in the section on wine samples analysis.