The benefit of using whole proteomes as QC materials is that the dynamic range of proteins in a proteome inherently covers many orders of magnitude, and the unattainable complete proteome coverage means that the performance scale should remain beyond instrument performance (that is until vendors begin promising complete proteome coverage), allowing future comparisons of performance across system upgrades or when acquiring new mass spectrometers. Whole proteomes are also useful since they can track the complete experiment from digestion to data analysis, and can be used with any protease. Having a material that includes extraction is valuable as extraction can account for the majority of technical variability . One notable proteome is the NIST RM 8461 (Human Liver for Proteomics), which is a relatively low-cost room temperature stable powder, still requiring lysis and digestion . There are other NIST materials containing proteins that may be similarly fit for purpose and universally available as well as commercially available lysates such as yeast (RM 8323 ), or not specifically fit for proteomics like NIST SRM 1950 (Metabolites in Frozen Human Plasma), which are ready to digest. It is important to highlight that Promega provides paired digested and undigested proteomes, thus an additional opportunity for QC (Table ).