Predicting 3D model for target Approximately 6% of the protein correlations in the human interactome, predicted to be researched experimentally, have been examined ( ). The scarcity of three-dimensional target models poses a significant obstacle in structural-based drug discovery and development. As a result, various techniques have been created to anticipate three-dimensional target models, one of which is Templatebased modelling (TBM). TBM predicts a protein model structure by using the known structures (Figure ). Several TBMs exist in the literature, including MODELLER ( ), SWISS-MODEL ( ), and FoldX ( ). The process entails aligning the sequence with homologous proteins with a known structure, selecting templates depending on the alignment quality, constructing a model using comparative modelling approaches, and refining the model to enhance its structural accuracy. Homology modelling allows for anticipating protein structures in three dimensions, helping develop structure-based drug design and other molecular investigations.