Metallic nanoparticles e.g., silver (Ag), gold (Au), palladium (Pd) , and platinum (Pt), advanced several applications. Silver nanoparticles have been used for many applications such as catalysis , energy , biosensing , laser desorption/ionization mass spectrometry (LDI-MS) and mass spectrometry imaging (MSI) , and others . In our lab, we investigated Ag NPs' antimicrobial activity against bacterial flora of bull semen .