Comparison with chromatography methods and NMR-DOSY. An estimate of molecular sizes can also be obtained with chromatographic methods. The chromatographic method that seems most similar to UV/Vis-DOSY is hydrodynamic chromatography, which is somewhat more difficult to operate than UV/Vis-DOSY, generally requires calibrants, and cannot so easily handle higher sample dimensionality (i.e. chemicals from different families). Taylor-dispersion analysis (TDA) is also commonly used for estimating the size of particles and molecules in solution, but mixed samples are not so easy to analyze with this method, and to obtain information on the electronic structure of the molecules coupling to a dedicated spectrometer is necessary. Hence, we think UV/Vis-DOSY can be a useful complement to these chromatographic methods.