To validate and explore the potential of MolDarting, we look at three different system with different requirements needed to sample binding modes. The first system explored is an alanine-valine dipeptide. While not typically considered a ligand, this peptide is a simple model system which exhibits three different stable conformations that vary by an internal torsion and can be slow to sample through plain MD. It also is a good test system for the darting approach we develop here, as MolDarting can be applied to any selected object in our system, not just a ligand. Here, since sidechains play an important role in ligand binding it is also important to be able to sample the rotamers in a binding site. The second system we look at with MolDarting is T4 lysozyme L99A with toluene bound, where the binding modes varies by rotation and translation. The final system we look at is HIV integrase with a variety of ligands bound. HIV integrase is an interesting test system because it has multiple binding sites where ligands can bind, and has proven difficult for binding mode predictions in a previous blind challenge, and we would like to test whether MolDarting can directly sample the binding modes in each binding site. In order to keep the ligand in the binding site while the ligand's interactions are off, an orientational restraint is used which corresponds to the dart that the ligand is in at the beginning of an NCMC move proposal. At the middle of the NCMC protocol, a MolDarting move is performed, and the restraint switches to a new orientational restraint corresponding to the new dart, which is subsequently turned off throughout the rest of the protocol.