Reinforcement of the GelMA precursor with PEC self-assemblies enabled 3D printing and biomanufacturing at a physiological temperature. The protective PEC hydrogel scaffolding which formed within milliseconds provided advanced structural robustness to the ink over hours prior to secondary crosslinking, making immediate photocuring upon deposition obsolete. Moreover, electrostatic reinforcement overcame current shortcomings of temperature-sensitive bioinks (e.g., GelMA) used for extrusion-based printing, particularly the remarkably deteriorating shape fidelity and resolution with increasing layer stacking due to the poor temperature transfer from the print bed to the top layers. In a proof-of-principle experiment, we printed a hollow square structure consisting of eleven layers and without photocuring between individual layers. For the bPE-free GelMA construct, we observed insufficient cooling of the top layers promoting inward bending and eventual collapse of the structure due to slow viscosity recovery and underlining the need of in situ photocrosslinking for GelMA after each deposited layer (Figure ). In contrast, GelMA inks supplemented with appropriate amounts of bPEs no longer relied on thermally induced pregelation by a cooled cartridge (22 °C) and print bed (17 °C) but on the instantaneous selfassembly of the bPEs. Hence, using PEC hydrogel reinforced bioinks enabled continuous fabrication at 37 °C of 3D structures with improved inter-layer adhesion and only a single photocrosslinking step in the end, thus leading to reduced manufacturing time and UV exposure to encapsulated cells. Increasing the level of complexity by printing a grid with a mesh size of 1.2 mm revealed similar improvements in printing performance -printing of 18 uncrosslinked layers without inducing fusion of adjacent segments (Figure ) was accomplished.