To probe the effect of capture affinity on complex distribution, capture strips -printed with 6 test lines of varying PSA concentrations (0.1, 0.5, 1 mg/mL) -were used to detect a high concentration of HER2 antigen (500 ng/mL). When examining the signal intensities for each test line, it was observed that strips printed with low receptor densities (0.1 mg/mL PSA test lines) resulted in similar levels of signal across all test lines. This highly uniform distribution of signal represents the ideal conditions for our 'capture-and-release' approach where a large receptor area circumvents poor test line affinity. In this scenario, a significant proportion of analyte target, which would otherwise go undetected in traditional LFA formats, can be realised through the AmpliFold 'capture-and-release' approach, whereby triggered release enables the distributed immunocomplexes to be rebound during detection strip in a concentrated manner. This is in contrast to assays under 'high sensitivity' regimes (1 mg/mL PSA test lines) where the majority of signal tended sharply towards the front of the capture area. Under these conditions, the benefits of a large capture area were diminished with signal distribution more closely resembling a typical LFA test line. In this scenario, the amount of additional target analyte recovered by employing a large capture area is less impactful, reducing the scope for signal amplification by 'capture-and-release', when compared to a traditional LFA format.