Furthermore, the LC solvent effect on resolution of the isomeric pairs was investigated using methanol (20% and 80%), acetonitrile (20% and 80%) and 5% isopropanol. The compounds were analysed by flow injection analysis (FIA) at flow rate 50 µL/min. With methanol, acetonitrile and isopropanol, CV values shift to more negatives values as SV increased (several compounds reach -29 V at SV=900V) typical for type A behaviour. Increasingly negative CV values with increasing SV is associated with strong interactions between the analyte ions and the carrier gas (solvent vapor) under low-field conditions. Regarding the organic content of methanol in the mobile phase, CV values shift to more negative using 80% methanol compared to 20% methanol (7 V difference for THC/CBD), but this is insufficient to baseline resolve these isomers. When using acetonitrile, the best resolution (ΔCV=8.3)