Fed Batch Bioreactor: One sample per day was taken from triplicate fed batch bioreactors, over a 14-day production from inoculation (day 0) to harvest (day 14), run under standard platform process conditions. Each sample was analyzed independently by two mass spectrometers, a cyclic IMS QToF (Waters Corp, UK) and a ZenoTOF 7600 (SCIEX, UK). 3599 proteins were identified on the Cyclic IMS QToF, 1234 proteins of which were quantified across the 14 days. On the ZenoTOF 7600, 4960 proteins were identified, with 2958 quantified (see Figure ! Reference source not found.A), an increase of approximately x2.4. Of the quantified proteins identified as having differential abundance, 834 proteins were found to be in common which is ~2/3 of the total identified with the Cyclic. Identified proteins may include some variants but are grouped to removed redundancies.