The cartridge was then rotated to a vertical position and allowed to run for 13 min 50 sec (Figure ). This allowed the EPI, DOX and DOL to sufficiently separate from one another. The cartridge was then rotated back down to a vertical position allowing the mobile phase to drain out of the TLC chamber stopping the TLC plate development and allowing the plate to be imaged using the PXI system for quantification (Figure and). Successful separation between the EPI, DOX and DOL in the patient samples as well as separation in the calibration curve sample was observed.