CVC measurements were performed on the four mixtures from AL 6 with the largest number of vesicles to better characterize the lower limits of formation. The critical point for vesicle formation was around 1 mM with the lowest value tested at 0.85 mM. These values are exceedingly low for single-chain amphiphile membranes. For example, the most well characterized prebiotic vesicles, formed from a mixture of decanoic acid/decanoate have the CVC between 10 and 40 mM. While these membranes were still orders of magnitude greater than that of stable phospholipid membranes (e.g., dipalmitoylphosphatidylcholine or DPPC is estimated to have CVCs in the sub picomolar range), this work demonstrates how active learning can find much lower CVCs for prebiotic amphiphile mixtures than previously known.