The biocorrosion is a great challenge and posed detrimental effects on the mechanical integrity of the metallic structures. In this work, the corrosion behavior of SS304 has been investigated in the presence of two bacteria strains i.e., PA-ZK and BS-S1X strains. Both bacterial strains were found to be hydrophobic as evaluated from the BATH and SAT. The degree of hydrophobicity is linked with the stability of the biofilm and proliferation of the bacteria on the surface of SS304. In other words, the presence of biofilm and bioactivity of the bacteria could affect the surface energy of the metal and may alter its hydrophobic character. The macromolecules i.e., lipids, polysaccharides, nucleic acids etc present in the extracellular polymeric substance (EPS) could interact with the dissolved metal ions and control the MIC. However, the stability of a biofilm and its interaction with the surface strongly depends on the nature of the EPS, and chemical composition, topography, and microstructural features of the alloy . After exposure to bacterial strains, the improved degree of hydrophobicity (~35%) corresponded to the barrier characteristics of the biofilms, which could be beneficial to reduce aqueous corrosion. For instance, the hydrophobic character is related to the barrier characteristics of the biofilm, which is validated by the limited ingress of aggressive ionic species at the biofilm/substrate interface. The barrier characteristics of the biofilm were evident in the impedance spectra (Figure ). The results reported in Table highlighted the relatively large resistance of the biofilm (Rbf) compared to the resistance of the MSM layer that could form on the surface of SS304 in the controlled medium. Similarly, improved corrosion resistance (as indicated by Rct values) was directly associated with the limited transport of the ionic species through the biofilm and interaction with the substrate . These Rbf and Rct values were obtained after fitting the impedance spectra by iterating the input values of each parameter in the EEC model with the help of Echem Analyst software (Gamry®). The results corroborated the interfacial electrochemical processes at the surface of the SS304 sample and barrier properties of the biofilm. For instance, in the S1 medium, the species i.e. glucose, amino acids and salts in the MSM could precipitate and deposit on the SS304 surface. These deposited species could form a defective surface layer through which electrolytes could penetrate and reach the substrate causing corrosion. Comparatively small values of Rbf (3.22 kΩ-cm 2 ) and Rct (4.85 kΩ-cm 2 ) exhibited by the SS304 sample in the S1 medium validated its poor corrosion resistance. On the other hand, in the inoculated media (i.e., S2 and S3), the relatively large values of Rbf and Rct were registered by the SS304 samples. For instance, the SS304 sample in the S2 medium registered relatively large values of Rbf (6.04 kΩ.cm 2 ) and Rct (11.14 kΩ.cm 2 ) compared to the low Rbf and Rct values of the SS304 sample in S3 medium (4.00 and 6.53 kΩ.cm 2 , respectively) as given in Table . These small values were attributed to the defective structure of the biofilm that was formed on the surface of SS304 by the BS-S1X bacteria strain. In simple words, the porous or defective structure of the biofilm could facilitate the ingress of ionic species to the biofilm/substrate interface. On the other hand, the formation of relatively compact and adherent PA-ZK bacteria containing biofilm (in S2 medium) hindered the penetration of electrolyte and restricted the progress of corrosion reactions at the biofilm/substrate interface as evident from the large Rbf and Rct values . The ingress of ionic species at the biofilm/substrate interface and defective structure of biofilm was further confirmed from the increased charge relaxation in the double layer that could form within the porous structure of the biofilm. This was evident from the relatively large Ydl (53.39 mS n2 /cm 2 )