Finally, we evaluated the total computational time for the streptavidin-biotin system. The computational time was approximately 13 µs (Table ). We recalled the experimental value of the dissociation rate, 1.05 × 10 )3 s )* , which corresponds to approximately 1,000 s as the dissociation time. We should emphasize that our CF-SMD simulations could predict the extensive dissociation time, which is far from the time that a conventional MD simulation can reach.