The DNA origami methodology published by Paul Rothemund in 2006 greatly expanded the possibilities of bottomup fabrication at the nanoscale . While the production pipeline of ssDNA scaffolds has improved since then, methodology for the design, synthesis, and amplification of long dsDNA (e.g. plasmids) is still much more advanced and widely studied. The utilization of dsDNA scaffolds has been starkly limited, because they are incompatible with Watson-Crick-mediated self-assembly. Here, we have demonstrated a general method for the programmable spatial organization of dsDNA by non-canonical DNA interactions. The success of triplex origami evidences that comparatively weak binding motifs, such as normal and especially reverse Hoogsteen interactions, can mediate the steep and frequent bending of a stiff polymer. Furthermore, TFOs that bind too weakly to form an isolated triplex (i.e. because they are too short or too low in G%) can function successfully as staples in a scaffolded system. We suggest that the success of HS-mediated scaffolded self-assembly is founded in a zipper-like folding cooperativity accumulated by multiple weak binders.