Template was removed from MIP film by immersing MIP-coated electrode for 60 minutes in 0.1 M carbonate buffer of pH = 9.1 under gentle stirring, and then the residuals of the buffer were washed out with Milli-Q water for 30 minutes. As a control, non-imprinted polymer (NIP) film was deposited the same way as MIP but without adding MTZ (Figure and).