Calculations such as absolute hydration free energy calculations may be a ected by di erent partial charge sets for a molecule when all other simulation conditions are held constant. We performed absolute hydration free energy calculations on 15 molecules, 5 "bad" molecules, 5 "good" molecules, and 5 "average" molecules. "Bad" molecules are those that had large maximal partial charge ranges. "Good" molecules are those that had small maximal partial charge ranges. For each molecule, we generated 5 partial charge sets from 5 random conformers using AmberTools AM1-BCC charges. We ran 5 AHFE calculations for each molecule where each calculation only di ered in their assigned partial charges; all other elements such as starting conformation and coordinates remained constant across repeats.