The development and optimization of the assisted reproductive technologies rely heavily on animal models, which offer valuable insights into reproductive biology and the effects of various ART interventions. Animal models are indispensable in ART research due to their biological and physiological similarities to humans and their role in studying species-specific reproductive processes. Moreover, animal models provide a controlled environment to study the mechanisms of reproduction, test new technologies, and assess the safety and efficacy of ART interventions. Thus, animals enable repeated experiments, ensuring consistent data collection; animal research minimizes direct experimentation on humans in the initial stages of ART development; certain animal species closely resemble human reproductive physiology, making them ideal for translational research; models help optimize ART for wildlife and livestock with unique reproductive traits. Furthermore, animal models allow for iterative refinement of techniques, provide insights into developmental biology and long-term effects, and enable high throughput testing of interventions. The development and application of ART in humans and model animals follow parallel tracks, with most techniques undergoing extensive testing in animals before being adapted for human use.