An atlas over the entire grid was collected as a low-magnification montage of 8×8 tiles. In the next step, typically more than 150 grid squares of interest were selected, and points were saved using "Add Points" in the SerialEM navigator window. After aligning the atlas with the magnification used in the medium magnification montages, one medium magnification montage of 3×3 pieces was collected at all saved points using the View mode. The "Rough eucentricity" and "Fine eucentricity" functions in SerialEM were used to automatically assign the eucentric height to each grid square that was stored with the corresponding maps. A point was added for each crystal of interest in the medium montage maps and saved in the navigator window. All points in the generated list were set up for data collection using the command "Acquire at items" function in the Navigator menu of SerialEM. Crystals ranging from 0.2 to 1.5 µm (size of the parallel beam) were picked for the data collection. Diffraction data using continuous rotation of the stage at 2°/s covering a total rotation range from -25° to +25° was automatically collected for each crystal using a SerialEM macro script. The script also used image mode to save an image of the content in the beam using the Search preset and was used to estimate crystal area and to visualize the crystal position during the MicroED data acquisition (Figure ). During the rotation, the camera integrated frames continuously at a rate of ~0.5s per frame, a total of 24.95s exposure time for 50 frames.