Dependency updates. Since we published the first ten talktorials in 2019, webservices and tools have December 8, 2021 changed or easier solutions have become available. This led to many updates in the existing notebooks, of which two are highlighted here. First, we are now using a combination of biotite and PyPDB to select structures and fetch structure files from the PDB to offer the same functionalities after the major update of the PDB webservices API . Second, in the initial release, PyMol was used for molecular visualization in 3D and structural alignment. To facilitate and expand operations in 3D, we now implemented a combination of NGLView to visualize molecules in 3D and OpenCADD to align structures in 3D.