SAMPL has encouraged participants to submit additional submissions as non-ranked, as this provides additional methods for cross comparison and opportunity for benchmarking. Additionally, this can allow for sensitivity analysis between variants of a specific method, and facilitate tuning or improvement of the method(s), force field, and/or protocol, while also highlighting successes and limitations. For these reasons, reference calculations conducted for this challenge include several variants of the method utilized, varying a single parameter in the approach (i.e force field, restraints used, guest orientation, etc.) to probe the impact on predictive accuracy.