Nanomaterials are defined as materials with at least one dimension that is between 1-100 nm in size. Their tensile strength, shape, size, surface area, charge, and easy functionalization make these nanomaterials suitable for various technological and biological applications. Nanomaterials, including single and multiple walled carbon nanotubes, DNA nanostructures, silica nanoparticles, and layered double hydroxide clay nanosheets, have been employed for delivery of nucleic acids (DNA and RNA) and other bioreagents into plant cells for diverse applications. Similarly, carbon dots were applied to deliver DNA into rice root cells and callus and small RNA molecules to induce RNA interference (RNAi) in mature leaves of model plant Nicotiana benthamiana (N. benthamiana) and tomato. Very recently, multiple reports have described the non-biolistic delivery of DNA into plant cells of model and non-model plant species without transgene integration using single-walled carbon nanotubes (SWCNTs) for transgene expression in mature leaf cell cytoplasm or inside chloroplast and pollen grains. SWCNTs have been widely adopted for the delivery of genetic cargoes. SWCNTs average 1-3 nm in diameter, and their high aspect ratios generate a large surface area to volume ratio for surface chemical functionalization and DNA binding. SWCNTs can be surface-functionalized with different molecules such as arginine or polymers, including polyethyleneimine (PEI) or chitosan for efficient DNA loading. In addition to their role in delivery, SWCNTs may also protect DNA from enzymatic degradation in the cellular environment. PEI-functionalized SWCNTs were recently shown to deliver DNA and express a green fluorescent protein (GFP) from a plasmid in intact leaf cells of different plant species. In these proof of concept studies, PEI-SWCNT conjugates were used to deliver a 4.2-kb plasmid carrying an enhanced GFP (mutant GFP, mGFP) cassette into the plant cell nucleus, where expression of mGFP was detected only in cells treated PEI-SWCNT-pDNA compared to cell treated with just plasmid DNA. Similarly, chitosan-SWCNT conjugates were used to deliver a GFP encoding plasmid into chloroplasts, where the release of DNA is favored by the basic pH of chloroplasts (pH 8), resulting in the detection of GFP by fluorescence microscopy in chloroplasts only. Thus, SWCNTs are considered promising carriers for the delivery of DNA plasmids and transient expression of transgenes, including CRISPR-Cas9 genome engineering machinery, into plant cells. However, most plant genome editing applications require efficient delivery and high expression of multi-functional large DNA constructs into the plant cell, often several times larger than reporter plasmids used for initial proof of concept studies.