On the other hand, the coarse isotope pattern generator calculates the unit mass isotopic patterns, using the summed probability for each isotopologue peak, ignoring the hyperfine structures. For this, a maximum isotopic tree depth was required that corresponds to one plus the maximum number of isotopes that could be present in a single molecule. Considering the fact that an increasing number of isotopes within a molecule results in a lower occurrence probability (i.e., intensity), a maximum isotopic tree depth of 6 was chosen.