Distinctive glycofluoroform fingerprint binding profiles for several protein binding partners were obtained by screening of a NGL-based microarray using a selection of analogues prepared in multi-milligram scale. A gold nanoparticle-based assay was successfully used to validate a subset of the microarray results. Many examples of increased binding affinity of Lewis x glycofluoroforms have been identified, notably between monoclonal Lewis x antibodies and bacterial toxins investigated, with different preferred fluorination patterns for different proteins.