A final experiment evaluated the effects of our inhibitors on goblet cells on small intestines isolated from mice treated with the inhibitors. Previous work by Faivre et al. demonstrated much less goblet cell toxicity from pan-D2 inhibitor ABBV-744 versus pan-BET inhibitor ABBV-075. We tested the effects of our inhibitors with different selectivity profiles on goblet cell toxicity based on Alcian Blue staining. In this short-term exposure experiment, mice were exposed to compound one hour prior to stimulation with LPS.