To ensure that students had a firm grasp of the background theory and experimental procedures, these were thoroughly explained and reviewed prior to conducting the assays. This approach proved effective in providing students with direction and confidence as they performed the assays. The class was divided into five groups, each investigating the effects of different regulators or components of the reaction. This encouraged meaningful discussions among students about the numerous factors affecting the signaling pathway and how to activate or inhibit certain effects. The students presented their findings and faced questions from their instructors and peers. Communication between and amongst groups was critical to their teamwork development and successful experimentation. The students learned how to operate instruments, collect raw data, and process it through Excel to create figures for their final presentation.