In our analysis we were trying to find the best soluWon for glucose analysis. As it was described in the introducWon, NEDC matrix is indicated as suitable one for glucose analysis in the negaWve ion mode and should resulted in a detecWon of a peak of m/z = 215.03. In our previous work we have tested the influence of the number of matrix layers on the glucose and lipid analysis using NEDC matrix and considering the peak intensity and signal to noise raWo, we indicated that for glucose analysis SunCollect nozzle at a height of z=5 (high posiWon above the Wssue), and 10 matrix layers is opWmal.