Figure shows the main effects plot for the four factors, where paired RP (blue) and HILIC (orange) data for positive mode is presented. Main effects plots for positive mode TIC and negative mode TIC/ number of features can be found in Supplemental Figure -S7 with consistent trends. The main effects plot visualizes the magnitudes of the factors for each level in relation to the average value. The points show the change in response at the various levels of each factor, with a dashed reference line drawn as the grand mean of the calculated S/N response. As the influence of a factor increases, the displacement of the observed S/N from the dashed reference line will increase, with larger divergence from the mean indicating a larger contribution of that factor/level. Consistent with the results from PCA analysis (Figure ), the extraction solvent followed by volume had the greatest magnitudes in the main effect plot for RP(+) mode. (66.5 and 65.6, respectively for RP+).