Flow Cytometry analysis of tagged TentaGel® M NH₂ Monosized Amino TentaGel Microspheres. 6-Carboxyfluorescein. Flow cytometry was performed on the tagged TentaGel® beads (TG-beads) using a BD FACSJazz TM Cell Sorter. The FACSJazz lasers were calibrated before the samples were run with the Rainbow Calibration Particles, 8 peaks (3.0-3.4 µm). Non-labelled TG-beads were dispersed in 5 mL sheath fluid and put through the machine to check the calibration. Then samples TGCFluor100, TGCFluor73 and TGCFluor36 were run through the FACS dispersed in 5 mL sheath fluid, being observed on laser 488 nm with filter 513/17 nm. All flow cytometry data was processed using BD FACS™ Sortware program.