Further, to account for the usage of many t-tests (282 t-tests, 1 for each residue), we applied the Benjamini-Hochberg correction , using a false discovery rate of 5%. This effectively means 5% of the features identified as significant will be false positives, whereas without the correction, this number would be notably higher. We note that the regions with the largest ΔRMSF values (~residues 220-230) in Figure , were not determined to be significantly different. This makes sense due to this region of the protein being highly flexible, and sampling large RMSF values in both enzymes (Figure ). This also showcases the importance in applying some form of statistical testing prior to interpreting MD simulation results such as these.