Additionally, 75 Na + and 42 Cl -ions were added to neutralize the system and provide the ionic concentration of about 0.15M. The obtained system was energy minimized with restraints placed on all atoms, except of water and with restraints placed only on the protein backbone (3000 and 2000 steps of steepest descent and conjugate gradient algorithms in each case). Next, the system heated up and equilibrated by running 100 ns in npt ensemble. This way a system of approximately 78.1 x 78.2 x 114.1 Å was obtained, which was used to perform further run of 6000ns in nvt ensemble to fully stabilize the system and obtain equilibration between protein and lipid bilayer. At the last step, three 1000ns trajectories of production runs were performed for two variants of UL49.5 protein: with uncharged and charged H67. For sake of comparison, MD simulation in the same lipid bilayer model was run for UL49.5 protein from BoHV-1 virus in the same scheme .