Lipopolysaccharide (LPS), a well-known inflammatory stimulus, induces reactive oxygen species (ROS) production in cells, increasing cytoplasmic viscosity and promoting inflammation. When cells were pretreated with LPS (1 μg/mL) for 30 minutes before exposure to the bismacrocycle 5, a marked increase in fluorescence intensity was observed (Figure and). This enhancement was significantly greater than that in the control group (Figure ), suggesting the bismacrocycle's potential for identifying and distinguishing inflammatory cells. The observed increase in fluorescence after LPS pretreatment may be attributed to changes in cellular viscosity, which could facilitate the radiative transition of the compound in inflammatory cells. This finding is important, as it highlights the bismacrocycle's ability to detect and monitor inflammatory responses within cells.