[125] This last strategy can be exploited not only with complete drugs but also with known active compounds against our target of interest.To follow the strategy of using bioactivity data the selected program has to admit this type of focused fragments, examples of softwares with this characteristic are LUDI (available with Discovery Studio) [126] and LigBuilder. It is possible that the software incorporates the fragmentation step from complete molecules entered by the user, like alvaBuilder (Alvascience, alvaBuilder (software for de novo molecular design) version 1.0.6, 2021, ). In case the virtual fragmentation has to be made before entering the information to the program it is necessary to construct a database of active modulators. The threshold value to separate actives and inactives can be established in 10 𝛍M as suggested by previous studies.