Non-invasive analyses were carried out to probe porosity insights of the cryogels from the macroscale to the nanoscale. Image analysis using Local Thickness was performed on SEM images of the cryogel scaffolds to obtain accurate estimates of minimum macropore sizes. "Local Thickness" is an algorithm for characterizing the minimum pore sizes of 2D or 3D pore structures by measuring the maximum diameter of a sphere that would fit the minor axis of visible pore spaces, while disregarding pore shapes . Dynamic vapor sorption (DVS) was performed to analyze mesopore data through equilibrium moisture (EMC) measurements. In DVS, a full cycle sorption desorption was conducted on every cryogel sample, and precise vapor pressure and gravimetric measurements were obtained. Vapor pressure and gravimetric measurements allow for the determination of EMC and the estimation of the volume distribution of mesopores.