The problem is that the Wssue is a highly complex matrix, and the method that works well using a dried droplet approach with a single substance and the stainless-steel underneath might not be as straigh}orward when we have glucose and a complex organic matrix around. It seems possible that something in the Wssue possesses exactly the same m/z value as glucose adduct, ionize in NEDC matric and is responsible for different ion map creaWon, or is responsible for different pa]ern of crystallizaWon.