Five hundred gram (500 g) of watermelon peel was measured using a weighing balance. The biomass were blended and mixed with water based on the experimental design values as stated. The essence of mixing the biomass with water is to allow the bacteria to move freely inside the fermenter (airtight container). The blended feed-stock was fed into the fermenter (airtight container) and closed after charging, so as to ensure airtight condition of the fermentation process. And it were allowed to ferment for different retention days at mesophilic temperature(with an optimum growth range from 20 to 45 °C ). After the retention period of each of the sample, the substrate were collected and filtered. Then a sample of the soluble product were taken for elemental analysis.