Many antifouling strategies have been presented above. The objective of this study was to identify a surface modification that would not have an impact on the electrochemical properties of various sensors to be utilized in the future. The selected method must not damage the catalyst, affect the reaction mechanism, or alter the reaction environment, such as through localized pH changes. Instead of external redox probe added to solution as in case of (ruthenium II/III hexaammine) redox couple our work is based on internal redox mediator adsorbed on the electrode surface. Syringaldazine was utilized as a model catalyst due to its easy adsorption onto carbon surfaces and its simple response to pH changes, as described by . Although the modification is very stable in buffer solutions, it rapidly deteriorates in more complex media, making it an excellent tool for measuring the advantageous effects of antifouling layers. This approach was used to screen more than 10 different antifouling layers, and evaluate both the protective abilities as well as the impact on the catalyst, which was not possible with an external mediator.