Multiple structural properties are accessible via MD simulation through direct observation, such as membrane thickness, surface area per lipid, and the total membrane volume. Naturally, these three properties are closely related, as the total number of atoms is fixed upon isomerization. However, the specific membrane geometry, quantified by membrane thickness and surface area per lipid, is influenced by membrane saturation and fluidity. We quantify the membrane thickness by tracking the distance from phosphate to phosphate between the two equivalent membrane leaflets. The all-cis membrane showed a lower thickness compared to the all-trans membrane (Fig. ), and the all-cis membrane has a larger area per lipid (Fig. ). The tradeoff between thickness and area is a known phenomenon from prior X-ray scattering experiments. Indeed, the area per lipid decrease of approximately 5-8 Å2 for most trans phospholipids compared with their cis counterparts lines up with prior simulations of less complicated membranes. The exception to this is cardiolipin, which has a much smaller change in surface area than the other lipids both in absolute terms and as a percentage basis, potentially as a result of better packing between the four fatty acid tails in native cardiolipin.