Fluorescent emissions were read every 1 to 5 mins over 2 hours. Wells containing substrate only (no inhibitor or enzyme) were treated as no enzyme controls (NEC). Wells containing enzyme and substrate (no inhibitor) were treated as positive controls. Average velocities were calculated for all wells (for points between 20 minutes and 1 hour) using Gen5 software. Percent activity for all wells were calculated and plotted in GraphPad Prism.