Consideration of Protocol-2 shows an increase in the tetrahedrality of the water molecules as the central water molecule has more partners ( Fig 5). But this increased in tetrahedrality in Protocol-2 compared to Protocol-1 is non-monotonic (Table ). As discussed earlier the value of 𝑞 𝑡𝑒𝑡 is dependent on the subtended angle 𝜃 𝑗𝑘 and we find a stabilization value of ~1kcal/mol by converting this to a free energy landscape that looks like a double well. This signifies structural disorder for bulk water, but this difference decreases gradually as the amount of reline increases in the system (Fig S7, supplementary information). However, this stabilization is negligible as it is comparable to the thermal fluctuations.