The smallest C2 aperture of 50 µm was selected, along with a spot size of 11, and a beam diameter of 30 μm to reduce crystal exposure and enable data collection without a beamstop. The diffraction data were gathered from a small region of the crystal (≈ 2 µm in diameter) using a selected area aperture. Any inelastic signal was removed using a Selectris energy filter with a 5 eV slit width. The data was recorded in MRC format, generating 840 4k x 4k frames in total, where each frame represented approximately 0.075° of data.