Note that while vesicle size can be tuned, having a size variation within a hydrogel might provide an advantage of enabling gradual compound release in response to a designed range of stress intensity, as the amount of stress required to reach the lytic membrane tension for different sizes will vary. Additionally, the functionality of the system can be further expanded when vesicles are used as nanoreactors or cell-like assemblies by encapsulating protein expression systems. We also envision incorporating stem cells into the mechano-responsive hydrogel so that differentiation behavior can be tuned in response to changes in hydrogel crosslink density or from the released chemicals when external compressive stress is applied. This simple approach of introducing mechano-responsiveness in hydrogels will find numerous applications in mechanobiology, tissue engineering, and regenerative medicine.