Spectrophotometer, Thermo Fisher Scientific (Waltham, MA) using a calibration curve obtained with known amounts of CUR, dissolved in ethanol. Samples were prepared in Rotilabo F-Type 96 well plates, Carl Roth GmbH & Co. KG (Karlsruhe, Germany) at a constant volume of 200 µL. Spectra were recorded from 300 to 700 nm at 25 °C. Curcumin absorption was detected at 430 nm. Prior to UV-Vis absorption measurements, the aqueous formulations were appropriately diluted with ethanol to give a final absorbance between 0.3 and 2.5 (diluted at least 1/20 (v/v)).