Patterson et al. used liquid cell TEM (LC-TEM) to understand the self-assembly and growth of ZIF-8 under different synthetic conditions and its effects on particle size . They established a relationship between the dynamics of particle growth with synthetic conditions at different electron dose rates and total accumulated doses, as well as at different accelerating voltages. Therefore, it is also essential to investigate the accelerating voltage's effect on the measured critical dose and the electron dose rate. Jain et al. explored the influence of graphene as the TEM substrate material. They studied the substrate's effect on prolonging the lifetime of the PbPc crystal structure during the TEM investigation . Since ZIF-8 is non-conducting, a conducting substrate like graphene could reduce the sample charging effect during TEM imaging. The effect of conducting substrate on the critical dose for ZIF-8 has not been studied so far.