The error on fitting Dʜʜ experimental data remains the largest for DPPC and DSPC at 333K and for PDPC and SDPC at 303K (Fig. : blue curves reaching a plateau away from reference black line). These four lipids have in common saturated tails including 16 and 18 carbons (PDPC and DPPC both include 16:0 tails and SDPC and DSPC both include 18:0 tails), indicating compromises had to be made during the optimization of the bonded parameters of these tails for matching experimental data set as target in different phase states and for the highly flexible PUFA-containing lipids. In the context of this rich training set, representative of the variety of PC lipids and including transversal experimental data, this result indicates there exists no set of bonded parameters allowing to further improve the matching of Dʜʜ thicknesses for these lipid models using Representation 2 (in the context also of the other optimization and modeling constraints: ranges defined for the exploration of equilibrium values and force constants, bonds and angles defined in the CG representation, non-bonded parameters and other simulation parameters used).