Results for the trypsin-benzamidine system when using HMR were fairly close to non-HMR and experimental values, yielding a koff of 310 ± 30 s -1 , a kon of (8.6 ± 0.7) ⨉ 10 6 M -1 s -1 , and a ΔGbind of -6.06 ± 0.08 kcal/mol. These results suggest that HMR does not negatively affect binding kinetics, at least for the trypsin-benzamidine system.