The thraustochytrid strain was first cultured in a 6 L bioreactor with glycerol as the primary carbon source. After cultivation for 5 days, the biomass was harvested and lyophilized to obtain dry biomass. The oils from a portion of this biomass (2.0 g) were then extracted by homogenization in a mixture of chloroform and methanol (2:1 by volume), based on a previously published protocol. Three extractions were combined, filtered, and concentrated under reduced pressure to provide approximately 1.0 g of oil (50% of the dry biomass). This oil was then subjected to a transesterification process to convert the triglycerides to their corresponding fatty acid methyl esters (FAMEs). The FAMEs were then analyzed by gas chromatography and identified by comparison to known standards. The most prominent fatty acids detected were the methyl esters of palmitic acid (C16:0) at 41% abundance relative to the total fatty acid content, docosahexaenoic acid (DHA, C22:6n3) at 29.0%, and docosapentaenoic acid (DPA, 22:5n6) at 11.7%. The relative abundance of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids (PUFAs) in the microalgal oil triglycerides were 54%, 1%, and 45%, respectively (Figure ).