A MALLS (DAWN Heleos II, λ= 785 nm, fused silica narrow bore flow cell,), coupled with the WyattQELS Module of the DLS detector (Dynapro™ NanoStar), and a differential RI detector (Optilab™, λ= 660 nm) all from Waters | Wyatt Technology Corporation (CA, USA) were used for detection. The separation was performed in a long separation channel (275 mm) equipped with a 250-µm spacer and a PES membrane (cutoff 5 kDa). The sample concentration was adjusted to approximately 0.1 % w/v and injected without prior filtration. The AF4 system used the following conditions: mobile phase, 50 mM NaNO3; detector flow, 0.5 mL min -1 ; focusing flow, 2 mL min - 1 ; inject flow, 0.2 mL min -1 ; injection volume, 50 µL; cross flow (Vx), 0.7 mL min -1 . For the detailed description of the applied cross flows during separation see Table . Data evaluation to obtain the number density, the particle size values (i.e., Rg,n, Rg,w, Rg,z Rg,avg, from MALLS; Rh,n, Rh,w, Rh,z, Rh,avg, from DLS), the shape factor , and the particle size distributions was done in Astra v7.3.0.