To ensure a thorough characterization of the purity of rAAV particles, we have meticulously designed a comprehensive two-pronged protocol. This protocol serves two primary purposes: first, it identifies and characterizes a range of contaminants that may affect the quality of the rAAV preparations, and second, it analyzes the empty-to-full capsid ratio. By evaluating these critical factors, we aim to provide an in-depth assessment of particle integrity, which is essential for the success of any downstream applications.