In contrast to the ladder molecules, the conjugated non-ladder molecules N1-PF6 and N2-PF6 showed significantly smaller average conductance values at 10 -3.8 G0 and 10 -4.8 G0, respectively. Moreover, the attenuation factor for conjugated non-ladder molecules N1-PF6 and N2-PF6 was observed to be 0.23 Å -1 , which is significantly larger than the conjugated ladder molecules L1-PF6 and L2-PF6. These results highlight the role of the ladder structure in molecular junctions for achieving relatively high junction conductance levels and minimal conductance decays as a function of molecular contour length. In addition to the primary conductance peak at 10 -4.8 G0, N2-PF6 also exhibits a small shoulder feature in the 1D conductance histogram around 10 -3.2 G0, which is attributed to an intermediate junction conformation before the molecule is fully extended as suggested by 2D correlation analysis (Supplementary Fig. ). The average conductance values of non-conjugated ladder molecules L3-PF6 and L4-PF6 were approximately 10 -4.9 G0. The lack of conjugation within these molecules significantly lowers their average conductance values by nearly two orders of magnitude compared to L2-PF6, which shows that continuous conjugation across the entire ladder structure significantly enhances molecular conductance. On the other hand, the non-conjugated, non-ladder molecule N3-PF6 exhibited a broad 1D conductance distribution showing a peak value around 10 -4.7 G0. Although 1D conductance histograms for N3-PF6 and L3-PF6 show similar peak conductance values, N3-PF6 only showed conductance signals at extremely small junction displacements around 0.2 nm (vide infra).