Male C57Bl/6NCrl mice, Inbred, SPF-Quality, Charles River, Germany between 8 and 10 weeks of age, ranging from 20 to 40 grams were used to study the pharmacokinetics of test compounds. On arrival and following randomization animals were housed individually in polycarbonate cages equipped with water bottles, unless contraindicated by study procedures (such as pharmacokinetic blood sampling) or clinical signs. Pelleted rodent diet (SM R/M-Z from SSNIFF® Spezialdiäten GmbH, Soest, Germany) was provided ad libitum throughout the study, except during designated procedures. The compounds were administered to the mice via a single intravenous (slow bolus) injection to the tail vein using a vehicle comprising DMSO, PEG200 and Water . Terminal blood samples were collected via aorta puncture following inhalation anaesthesia into K2EDTA tubes and stored on wet ice. Oral cohorts were dosed by gavage using a vehicle of 0.5% (w/v) methylcellulose and 0.1% (v/v) Tween80 in water with bloods collected using a similar procedure. Whole blood was processed to plasma by centrifugation (3000g for 10 minutes at 5°C) within 30 minutes of collection. Plasma samples were transferred into 96 well plates (matrix tubes) and stored at < -75°C. Following termination, brains were collected from the animals and the cerebella separated. Both tissues were rinsed with saline, weighed and stored at ≤ -75 oC prior to analysis using LC-MS-MS.