A steady-state kinetic assay was performed to understand the kinetic characteristics of the OA nanozyme (Figure , 3G),. To enable accurate kinetic analysis using a coloring substrate (e.g., ABTS), the initial substrate concentration was lowered. In this case, the H2O2 concentration was reduced to one-tenth of that used in a previous colorimetric assay to prevent the fast oxidation of the coloring substrates from reaching the saturated point. By obtaining catalytic information, the kinetic profiles and their parameters were calculated using the conventional Michaelis-Menten equation and Lineweaver Burk plot for further analysis of the kinetics behavior of the OA nanozyme.