Using the first 100 PDE2a synthetic trajectories, we verified that the maximum effective sample size heuristic implemented in PyMBAR's timeseries module gave identical results to the implementation in RED. We then compared the truncation points selected using this method and the equivalent minimum marginal standard error heuristic (see Equations 18 and 10), where we did not employ the adaptive integration scheme described by Chodera et al.. As expected, the truncation points selected were generally very similar: identical truncation times were selected in 81 % of cases and the difference was less than 0.1 % (of the total time series length) for 95 % of the trajectories. The minimum standard error method showed a slight bias for later truncation times over the maximum effective sample size method -the mean (ESS -MSE) difference was -0.34 % of the total time series length. However, we found that using the marginal standard error method fixed several problem cases where time series