2b. Electric Field Implementation and Selection. At the beginning of production runs, a static electric field was applied in the x direction with no component in the y and z direction. A static field was chosen as the interaction between the molecule and the electric field would be mainly due to its classically defined permanent dipole such that higher order dipole terms, which are quantum mechanical in nature, and hence not captured in the GAFF /CNDO 33 + SPC/E molecular force field used in this work, can be neglected , allowing classical MD to be performed. Furthermore, the large permanent dipole moment of the glycine zwitterion (9.98 D with CNDO charges) and SPC/E water molecule (2.35 D), suggests that the effect of the electric field is accurately determined by classical mechanics where the electric field interaction with the permanent dipole is far greater than its induced dipole and other higher-order polarization counterparts. In the case of water, recent molecular simulation investigating into the polarizability of water under static fields have demonstrated that overall dipole moment of liquid water will only vary by 1% when subjected to a static field intensity of 5 V/nm . In this work, an exhaustive electric field screening was performed by simulating static electric field strengths of 0 V/nm (no field), 0.1 V/nm, 0.2 V/nm, 0.3 V/nm, 0.4 V/nm, 0.5 V/nm, 0.6