At MIC, lead cyclic peptides 15c and 16c showed good anti-biofilm activity against MRSA (Figure ) and E. coli (Figure ) with around 41-49% decrease in biofilm mass compared to non-treated cells. For both 15c and 16c, at 2× the MIC, a higher anti-biofilm activity was observed with a 63-69% decrease in biofilm mass. Noticeably, compared to 15c and 16c, slightly less antibiofilm activity was observed for daptomycin, with around 33% and 49% decrease in biofilm mass of MRSA at MIC and 2× the MIC, respectively (Figure ).