Among the low total concentration region (< 3 mM), those samples that had high probabilities of forming vesicles were from 88-96% total decyl trimethylamine and decyl sulfate compared to those samples having low probabilities of finding vesicles (Figure , top half samples). The ratio of decyl trimethylamine to decylsulfate ranged from about 1:1 to 1:4 in vesicle-forming samples. Moreover, finding vesicles in the region (< 3 mM) is very difficult.