We proved in the previous section that compound 38 could bind to NSD2-PWWP1 domain. To further detect whether compound 38 can affect HK36me2, we adopted AlphaLisa which was designed for screening NSD2 enzymatic inhibitors. It was shown that compound 38 did not display inhibition effect on H3K36me2 in vitro (Figure ). Consistent with Alphalisa results, compound 38 caused little effect on general H3K36me2 in cellular level (Figure ). These results are consistent with the findings from the study of Dilworth et al., as they pointed out that PWWP1 antagonism by UNC6934 did not have a significant effect on ribosome transcription or global levels of H3K36me2. In conclusion, compound 38 had no impact on SET domain or enzyme activity catalytic function, which further validated that compound 38 could specifically binds to PWWP domain.