Several aspects of the current research could be further expanded on. First, the application of enzyme-based methods to remove mycotoxin contaminants from food remains limited by high economic costs. Although degradation pathways comprising multi-step reactions can achieve complete toxin degradation, the present study used an optimal single-step reaction to degrade mycotoxins into products with low toxicity, considering economic factors. However, the screening framework can also be combined with pathway design tools, such as novePathFinder 37 , to develop more comprehensive degradation pathways for pollutants or synthetic pathways for high-value chemicals. Second, similar to lead identification in drug discovery, the discovery of lead enzymes (natural enzymes with moderate activity) is the initial and rate-limiting step of the research and development cycle in enzyme engineering and, in most cases, a prerequisite for the application of directed evolution and rational design. This study focused on the screening of lead enzymes rather than the improvement of enzyme activities via structural modification. Third, although we successfully synthesized all the tested enzymes, the potential for protein expression failure remains because the correct folding of some proteins may not be observed in the CFPE system; researchers will need to consider the applicability of CFPE systems when using the screening framework. Nevertheless, we believe that the present study provides an indispensable tool for the efficient identification of new enzymes with expected catalytic activity.