This model was also used to screen the Maybridge library of compounds to identify a small and diverse selection of molecules to evaluate in biochemical assays. The results were filtered manually to give a final selection of 18 compounds that were subsequently evaluated for their effects on the parasite's internal Na + concentration (at 1 M) and pH (at 5 M). None of the compounds were found to increase the parasite's Na + concentration or pH, which confirmed that the model required further optimisation and led to the start of a crowdsourced attempt to solve this challenge.