Similarly, for 6, the native hBChE in the control sample featured a mass of 60073 ± 1 Da (4 Da difference to the ProtParam 61 calculated mass) and the 6-incubated sample produced a species with mass of 60144 ± 1 Da (Figures ). The mass difference of 71 ± 1 Da, is in good accordance with the expected mass difference for the dimethylcarbamoylated enzyme (72.1 Da).