Comparison of dSPE and LLE performed using Folch, BD, and Matyash methods demonstrated similarity in the recovery distributions as shown in Figure . However, a greater number of LMWMs detected in the LLE methods had lower recoveries (<100%) compared to the dSPE method. Notably, the Matyash protocol demonstrated the most comparable performance to dSPE amongst the LLE methods tested. However, LLE methods typically require a larger investment of time and resources for sample preparation, and the observed lower recoveries of LMWMs render them less favorable for lipid removal, especially when downstream analysis of the hydrophilic fraction by C18 RPC is desired.