5.6g of Ferric chloride hexahydrate and 6.7g of ferrous sulphate were dissolved in 50ml of 0.5M HCl. 10mL of the prepared Hexane extract was added. 90 ml of 1.5M Sodium Hydroxide was added dropwise to the solution which is under vigorous mixing on a hot plate stirrer set at 80degree Celsius. The pH of the solution was observed to be highly basic (pH 12), on obtaining a viscous and jet-black colouration, transfer the solution to 50 ml centrifuge tubes. Around 83mL of Sodium Hydroxide was needed to obtain the viscous jet-black coloured precipitate. Centrifuge the solution at 2000 rpm for 2 minutes. Followed by the washing step where we discard the supernatant and add 10 ml of distilled water. Perform the washing step 5-6 times until the pH of the solution is neutral (pH 6.5). Make sure the sides of the centrifuge tubes are sealed with Aluminium foil to prevent light absorption as Thymoquinone is light sensitive. Finally, discard the supernatant and transfer the pellet to a Petri plate and place it in a hot air oven set at 60 degrees Celsius for strictly 8 hours to avoid crystallization. Scrape out the particles using a sterilized spatula and store it in a 1.5ml sealed tube away from sunlight. A strong odour of Nigella sativa seeds was felt during scrapping.