Staphylococcus aureus (S. aureus), and Pseudomonas aeruginosa (P. aeruginosa). It offered minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 3.125-12.5 and 3.125 μg/mL, respectively. There was no detrimental effect (P ˃ 0.05) on the percentage of sperm motility, plasma membrane integrity, acrosome integrity, and normal sperm morphology at concentrations of 15-30 μg/mL. Ag@C NPs is a promising antibiotic agent for bull semen extender during cold storage. It can be used in applications such as the field of artificial insemination . The antibacterial activity of silver ferrite (AgFeO2) was investigated. AgFeO2 was modified with polyethylene glycols (PEGs) to render their dispersion high . The antibacterial activity against pathogenic bacteria was quantified using plate counting, and the turbidity using optical density at wavelength 600 nm (OD600).