Among the mill types evaluated, SU mills, especially at lower operational speeds (2,000 rpm), were found to provide an optimal balance for extracting higher levels of cannabinoids. This suggests the importance of selecting appropriate mill types and operational settings for the extraction and analytical evaluation of cannabis. The findings regarding the 1.4L container highlight that certain mill types or operational parameters might be less effective in preserving cannabinoids than others. It is postulated that the lower cannabinoid recoveries for the 1.4L container is due to trichomes sticking to the side of the large volume container and resulting insufficient transfer to the sample vials. Additionally, the study reveals no significant differences in decarboxylation and oxidation across various milling instruments and protocols, indicating minimal concerns about oxidation and the formation of cannabinol (CBN) within these milling experiments.