For COC substrates, it is known that polar organic solvents cannot dissolve the polymer while nonpolar organic solvents such as hydrocarbons can dissolve it well 106 . As an example, in order to bond COC channels to a COC flat layer, the flat layer could be exposed to saturated methylcyclohexane vapor for 15 s at 30 ℃ followed by immediate contact to the COC channels at 85 ℃ for 15 min under 1 MPa pressure . In another study, a COC lid was immersed in 15% decalin diluted in ethanol for 5 min to plasticize the COC. The layer was brought into contact with another COC layer with 1.7 MPa pressure at a temperature below the glass transition temperature . Bruijns et al. exposed the COC substrates to a solution containing 40 vol% cyclohexane and 60 vol% acetone for 2 min . Afterwards, the substrates were bonded together at room temperature under a weight of 2 kg. PC-PC bonding has also been studied via solvent bonding using an acetone and n-pentane mixture (Figure b) . The process initially involved immersing two PC substrates, one with patterned microfluidic channels and one without, into a solvent containing acetone, n-pentane, and 1H,1H,2H,2H-perfluorooctyl trichlorosilane (FOTS) . The n-pentane solution acts as a sacrificial solvent and evaporates faster leaving temporary high concentrations of the acetone for dissolving the substrate. The substrates were inserted into a heat roller with a pressure of 0.1 MPa, which results in the mobilized polymeric chains being entangled. The use of FOTS provides hydrophobicity after the bonding, which is not normally achievable as the surface energy of the substrates are usually increased in the bonding process to obtain better adhesion. Mirgissa et al.