Four impact categories are quantified with the Product Environmental Footprint 2.0 (PEF 2.0) method as it is recommended by the European Commission ; Manfredi et al. reported that PEF 2.0 provides a good consistency, comparability and reproducibility of results . Within our LCA model we chose to quantify climate change (CC), acidification potential (AP), land use (LU), and water use (WU), as these impact categories represent the largest environmental burdens from CNC production due to high energy and water requirements, use of renewable resource and acidic nature of the process. Table summarizes the information of the LCA method used in this study.