In this review, we presented several electrochemical DNA-based sensors for the detection of general E. coli and STEC were developed since 2015. This review highlights the impact of the electrode material, electrode modification, and the detection technique in the process of the DNA based biosensor development. Sensitive and selective biosensors have been developed by many researchers . Many of these studies have used gold, ITO or carbon electrodes or magnetic nanoparticles. Among them the gold electrodes are the most common used substrates since gold surfaces provide high conductivity, and easy modification with thiol modified probe DNA. The gold electrode surfaces were either directly modified with thiolmodified probe DNA or firstly modified with a mix of polymers and nanomaterials allowing a covalent attachment of probe DNA. These modifications allow high sensitivity of developed biosensors due to increased conductivity, however, they also increase the complexity and preparation time of the biosensors due to additional fabrication steps. Such modifications are difficult to achieve at manufacturing scale with high reproducibility for commercialisation.