The stability of the system was assured by conducting potentiostatic reading at a fixed potential against open circuit potential (OCP). The first 30 minutes duration was measured with only DMEM in the well, which was then spiked with HeLa cells and their attachment on to the electrodes were observed for 60 minutes. Later, trypsin was introduced to the well in order to track the detachment separation of these cells from the electrodes and studies were continued for an additional 30 minutes. Any current or voltage input to the setup in the absence of cells with only the medium as an electrolyte is merely interfacial impedance in series with ionic solution conductivity. As anticipated, our results showed lower resistance values and a more relaxed growth pattern during the first 30 minutes of recording compared to growth while the cells were making close contact with the electrode surface. This attachment of the cells results in a barrier that the impedance signals must cross before achieving the solution resistance. The resultant output is a combination of resistive and While the insulating cell membranes are responsible for the capacitive effects, the resistive effect is due to the ionic properties of cytoplasm . Although inspection of cell responses for 30 minutes after the addition of trypsin did not completely remove them from the substrate contact, reducing the cell layer resistance to the point where the final signal resembled those throughout the absence of cells, it was evident that within this short period of time a considerable portion of the cells was successfully detached from the electrode contact. The responses of HepG2 cells were also studied through recording impedance alteration over time. It was noted that the resistance component Z' does not change significantly because there are still noticeable intercellular voids. HepG2 form spheroids on the surface of culture vessels rather than confluent layers as HeLa cells. What is more, with this cell population, the adherence interaction did not appear to be strong enough to give considerable change in Z'. However, when it comes to the capacitance component, Z'', accountable changes can be seen within the measurement conditions. Thus, the Z'' element validates to be more dependable variable for us. The figure illustrates the real-time monitoring of the impedance evaluations for 2D HepG2 cell culture. Comparable signals were obtained from all 8 electrodes.