The altered expression and activity of system x c -in cancer has enabled the use of [ 18 F]FSPG for tumor detection in various cancer types . To facilitate the use of [ 18 F]FSPG in preclinical and clinical studies, reliable automated synthesis of the radiotracer is necessary. Our aim was to develop an automated procedure for the synthesis and purification of [ 18 F]FSPG on a single synthesis module that would yield [ 18 F]FSPG in sufficient radiotracer concentration for preclinical imaging, even when starting with low amounts of radioactivity were used. To facilitate rapid translation of this methodology and distribution to multiple sites, we selected the GE FASTlab for automated radiosynthesis. As one of the most widely used synthesis modules for clinical production, the FASTlab is well-equipped to enable rapid cartridge based purifications suitable for GMP synthesis and additionally has been used for the automated synthesis of a variety of 18 F-labeled radiotracers .