For peptide mapping, non-deuterated HDMS E files were processed via ProteinLynx Global Server (PLGS) ver. 3.0.2 (Waters Corporation, Wilmslow, UK) with low energy and elevated energy count thresholds of 250 and 100, respectively. The primary digest reagent was set to non-specific with 0 missed cleavages and no false discovery rate filter. Peak lists were database searched against the target protein(s) and pepsin sequences. For manual drift FWHM trendline input, the Apex3D/Peptide3D executables were operated via windows command prompt. Identical parameters were used but with the addition of manually inputted driftFWHM-start and -end arguments. Peak lists were then database searched against target protein(s) and pepsin sequences by importing to PLGS.