Ideally, the sign of Δτ is expected to switch when ∆𝑐̃= 0. However, the results imply that the sign switches between ∆𝑐̃= -0.5 and -1.0 (i.e., ∆𝑐̃≠ 0). Here, we define an offset concentration of this molecular comparator, 𝜎, at which the sign of Δτ switches, where the output of the comparator switches. Regular electrical comparators generally have a non-zero offset voltage because of non-ideal circuit properties; similarly, our molecular comparator has a non-zero offset (𝜎 ≠ 0). 𝜎 ≠ 0 would be observed probably because B-droplet division took longer than that of the C-droplet for some reasons; for example, the DNA sequence difference induced the slower cleavage of L † AB than L † AC, or more linker cleavage is required for B-droplet division than C-droplet division. In future studies, 𝜎 may be tuned by sequence designing of DNAs.