The KcsA 1k4c homotetramer model was chosen to apply DW-BEL and subsequent ADV, because it codes for the open-state that favors K + flow and also have shown to be an inhibitor target. To begin our explorations, two previously proposed binding sites were chosen on the 1k4c model, i) the central cavity below the ion-sensitivity filter, targeted by alkyl-qac inhibitors (Figure , central blue sphere) and ii) the four lipid shallow-grooves of monomer interfaces formed by the external surface of the homotetramer (Figure , red spheres).