To preliminarily evaluate of the risks associated with the use of ILs and DSILs with surfaceactive amphiphilic cations, an acute toxicity analysis was performed using a model organism from the freshwater algae group, C. vulgaris. The study was performed for both parent ILs, A and B, as well as DSIL AB0.2. On the basis of the analysis of the obtained dose-response curves, EC50 values were determined for each of the tested systems (Fig. and Table .15 in the Supplementary Data). significant effect on ecotoxicity. Compounds from the auxin group are also produced by other groups of organisms, including bacteria, fungi or algae . It is postulated that auxins in algae play a similar role to those in higher plants, stimulating the cell division and growth of algal colonies . Consequently, herbicidal substances from synthetic auxins (including MCPA and dicamba) are characterized by low toxicity to algae . Cationic surfactants are known for their high toxicity to algae , primarily because of their ability to adsorb onto their cell membranes .