Quaternary ammonium compounds like benzalkonium chloride, 2-poly(ethyl alcohol), chlorobutanol, and parabens are typical preservatives that meet most of the antimicrobial required criteria. As stated Gupta, Mishra in their study that preservatives in ocular formulations harm the epithelium, yet they are important in multi-dose containers. Since BAC causes ocular cytotoxicity, Polyquaternium-1, sodium perborate, and stabilized oxy chloro-complex are being investigated. For individuals with severe allergies or surgical circumstances, a preservative-free single-dose bottle was employed. Preservative-free containers must be sanitized and stored properly to prevent microbial growth.