Plant metabolism encompasses a complex network of biochemical reactions that are essential for growth, development, and adaptation to environmental conditions . Primary metabolic pathways, such as photosynthesis, respiration, and nitrogen assimilation, provide the basic building blocks and energy for cellular processes. Secondary metabolic pathways produce specialized compounds that play roles in plant defense, signaling, and adaptation . Understanding the regulation and flux through these pathways is crucial for successful metabolic engineering. Recent advances in metabolomics and flux analysis techniques have provided unprecedented insights into the dynamics of plant metabolism .