The difference in the maximum P(active) value between one position and another reveals that trisynthons are more sensitive to the building block present in certain positions. We posit that in this DEL where trisynthons are synthesized linearly (Figure ), position 3, being the furthest from the DNA barcode and therefore the most exposed, has the greatest effect on overall activity. Position 1 has the smallest effect on overall compound activity as the position closest to the DNA tag. Since DELs are typically screened with DNA tags still attached, we believe the presence of the DNA tag may partially obstruct interactions with the target.