Extrac on of microalgae triglycerides. The lipid extrac on procedure performed in this work is based on the method developed by Folch et al. and is executed under yellow light condi ons. Prior to extrac on, 1.5 g of lyophilized biomass were placed in 30 mL hexane with 0.01 % BHT (1:20 m/v) for 60 h and sonicated every 24 h for 60 min to disrupt the cell membranes and maximize lipid extrac on yield. Next, the biomass was homogenized using a high-performance dispersing instrument (IKA T25 easy clean control ULTRA-TURRAX) at 12000 rpm for 10 min in an ice bath. The homogeniza on step was repeated three mes, and the extracts were then filtered. Then, 6 mL of a 0.88 % KCl solu on (5:1 v/v) were added to remove non-lipid contaminants. The aqueous phase was discarded, and the organic solvent was removed under reduced pressure. The desired extract was obtained as a viscous, dark green oil. Note that the hexane used for extrac on can be collected and used for further extrac ons to avoid unnecessary organic solvent waste. The microalgae extracts were dissolved in chloroform and stored under nitrogen at -20°C to avoid any hydrolysis and oxida on processes.