Proteomics. Isolated EV samples and cells (cleaned from FBS) were freeze-dried and sent to Proteome Center Tuebingen where the proteins were degraded with SDS, reduced by DTT, cleaned with S-trap cleaning and digested with trypsin before loading approximately 500 ng of sample onto the Thermo Scientific Q Exactive HF Orbitrap LC-MS/MS System and measured with a 60minute gradient, top7, sensitive method. Analysis was done using Max Quant version 2.2.0.0 against human database from UniProt (). Proteins were then categorized according to their cellular locations which can be seen in the supporting information Table .