The interest in lipid nanoparticles (LNPs) has increased tremendously because of their large potential in vaccines as a delivery platform. However, the understanding of this technology is still substantially lacking and remains a multivariate problem, making it difficult to investigate and decouple specific variables. An LNP typically consists of five major components, an ionizable lipid (IL), a sterol, a helper phospholipid, polyethylene glycol conjugated (or PEGylated) lipids, and cargo. In addition to optimizing each component, the relative ratio between them can also be optimized depending on the cargo, target cell specificity or stability, not to mention the assembly process . Although experimental studies can explore certain variations under certain conditions, extensive screening of ratios and components is neither easy nor cheap, especially when non-commercially available lipids need to be synthesized and tested .