In the chemically fueled assemblies design through the charge abolishment strategy, we reasoned that the negatively charged precursors repel each other to overcome the attractive forces that lead to their disassembly. Based on the above data, this is not the case. We hypothesized that the precursor in the assembly might not be as anionic as we initially suspected. Upon deactivation of the product in a fiber of Fmoc-AVD, the negative charges on the precursor's carboxylate are not reinstated. This mechanism is induced by a drastic shift in the pKa in the microenvironment of the fiber. In other words, the precursor in solution is deprotonated, which prevents its assembly. However, it remains (partially) protonated in the fiber, and thus charge neutral and unable to disassemble (Fig. ). We measured the pKa of the Fmoc-AVD, and Fmoc-GGD and compared Ac-FAVD, a peptide that does not assemble and has pKa's of 2.2 and 4.2 (Fig. , Fig. ). For Fmoc-GGD, we found only one pKa at 4.4 by titration of HCl. Interestingly, when the pH was close to the pKa, the solution turned turbid. In other words, the measured pKa was of the precursor in an assembled state, which was likely different than the pKa of the precursor in solution. In contrast, for Fmoc-AVD, we found a pKa of 5.8, i.e., far higher than the non-assembling Ac-FAVD (Fig. , Fig. ).