Further, we tested the changing LNP morphology dependent on the protonation level of Lipid 2. We used the initial structure of fully protonated LNP with Lipid 2 and deprotonated a fraction of Lipid 2 (Figure ), keeping the system net-neutral (replacing the required amount of Cl -ions by water beads) and simulated them for 10 𝜇s with annealing. The protonation or deprotonation of 10% of ILs did not make a significant difference in the LNP structure. Deprotonation of more