PBA-DLA copolymer was enzymatically synthesized using CALB as a biocatalyst and its chemical structure and potential usage in the biomedical field were evaluated. Performed work indicated on successful encapsulation of hydrophobic α-tocopherol -one of the determinative forms of vitamin E, using novel biocompatible PBA-DLA copolyester. The NPs were prepared by nanoprecipitation method and DLS measurements showed that by using neat PBA-DLA (without α-TP and stabilizer) it is possible to produce narrowly distributed particles with a particle size of ~202 nm, however, NPs tend to agglomerate during purification (centrifugation) step and therefore PLUR stabilizer was used to eliminate this drawback. Optimization of the experimental conditions, mainly the concentration of PLUR stabilizer resulted in the formation of α-TP-loaded NPs within the hydrodynamic diameter range of ~146-176 nm. NPs size was increasing with higher α-TP concentration which according to our speculations is related to the higher amount of active ingredient located on the surface of the polymer shell. Furthermore, based on the calibration curve prepared using UV-VIS spectroscopy, the α-TP encapsulation efficiency (EE%) was calculated. Performed studies indicated that the highest EE% values were reached by using α-TP concentration of 5 mg/ml (74 %) whereas in case of 2.5 and 10 mg/ml EE% was calculated to be ~50 %.