Collectively, the data package forms a strong foundation for the development of more potent FabF inhibitors. In addition to the fragment libraries, we have tested over 80 analogues of the hit compounds for binding and could determine crystal structures with 8 of those bound. As discussed, growing some of the fragments into the direction where the fused ring system of platensimycin and platencin is placed appears to be a viable strategy. Further, adding a covalent warhead to the fragments located in the fatty acid channel to enable a covalent attack of the active side Cys is attractive. Finally, it remains to be seen if inhibition can be achieved by binding into the dimer interface close to the fatty acid binding site. We are currently exploring some of these options with the goal to deliver more potent FabF inhibitors with antibiotic activity.