To see how these changes affected the model, the different CG models' bonded parameter ϵ did not affect the bonded parameter distribution significantly. Therefore, to be consistent with the MARTINI DNA bead types, σ was chosen to be 0.34 nm. The lowest possible ϵ value that resulted in a nanotube structure by the end of a 2 ns simmulation was tested and the value was 0.75 kJ. The bonded parameter distributions for this combination is shown in Figure , with and without explicit harmonic hydrogen bonds. For the average bond lengths, to account for differences between bond lengths in AA simulations and that in CG simulations, the CG average bond lengths were divided in half. This resulted in a more equitable comparison between the two simulation scales. The average angles and average improper dihedrals for the CG simulations were compared directly to those of the AA simulation. Since the explicit harmonic hydrogen bonds did not change the distributions in a significant manner, the final CG model does not include them. For clarity, Figure only shows the results for the σ = 0.34 nm and ϵ = 0.75 kJ systems with and without explicit This CG model with σ = 0.34 nm and ϵ = 0.75 kJ was further analyzed. The various characterizations of this nanotube were explored via parameters such as curvature, torsion, axial rise, etc. Similarly, this code can be found on the author's GitHub. The parameters of interest were the inner and outer radii, the axial rise, curvature, and torsion. To be as accurate as possible, the inner and outer radii took into account the size of the MARTINI beads. The axial rise was the distance between the two rosette rings. The curvature is how much the outer surface of the RNT deviates from being a plane. Mathematically, it is shown by Figure : The average bonded parameter distributions for the bond lengths (top), angles (middle), and improper dihedrals (bottom). The histograms are shown in the thinner lines for each system and the average distribution is shown in the thicker line. Each CG system was compared to the AA system (in black), and only the results of the σ = 0.34 nm and ϵ = 0.75 kJ are shown here. The darker green line is the system without explicit hydrogen bonds and the lighter green is the system with explicit hydrogen bonds during the simulation.