Hence, for HRTEM image acquisition, an electron dose rate of about 1-5 e -Å -2 s -1 over a short exposure time should be used. Increasing the accelerating voltage to 300 kV enhances the critical dose of the Bragg planes in the ZIF-8 crystal. No significant effect of graphene as a conducting substrate was observed at any accelerating voltage. Cryo temperatures seem to mitigate the electron beam-induced damage and considerably improve the critical dose compared to room temperature measurement at 300 kV. No significant effect of sample drying in a vacuum has been observed.