Here, we report the caged subtype selective serotonin receptor agonist and antagonist for serotonin receptor 5HT2C. The agonist, WAY161503, is caged with a green light responsive BODIPY photocage, and the antagonist, desmethylclozapine, is caged with a red light responsive BODIPY photocage. We show that we can use a green laser to provide agonist and red laser to unveil the antagonist and demonstrate this in 293T cells expressing 5HT2C. This spectral multiplexing also permits us to use both probes in the same experiment, using a green light to deliver an activating bolus of agonist and a red light to deliver a deactivating bolus of antagonist. We show that this may be used to target single 5HT2C transfected 293T cells such that can observe a rise in a stimulationinduced intracellular calcium flux which is then quenched with the uncaging of the antagonist.