Here, we present a versatile and simple method for the sequence-directed folding of double-stranded DNA into desired shapes by triplex-forming oligonucleotides. We demonstrate two distinct approaches for dsDNA plasmid sequence design; one geared towards scalability of 2D and 3D lattice-based structures, and the other prioritizing freeform physical manipulation of dsDNA. Both of these rulesets work nearly interchangeably for both the normal Hoogsteen (nHS) and the reverse Hoogsteen (rHS) binding motifs.