LC-MS analysis was performed by the FingerPrints Proteomics Facility (University of Dundee). Analysis of peptide readout was performed on a Q Exactive™ plus, Mass Spectrometer (Thermo Scienti8ic) coupled to a Dionex Ultimate 3000 RS (Thermo Scienti8ic). LC buffers used are the following: buffer A (0.1% formic acid in Milli-Q water (v/v)) and buffer B (80% acetonitrile and 0.1% formic acid in Milli-Q water (v/v). 15 µl of each sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 A| , Thermo Scienti8ic) equilibrated in 0.1% TFA. The trap column was washed for 3 min at the same 8low rate with 0.1% TFA and then switched in-line with a Thermo Scienti8ic, resolving C18 column (75 μm × 50 cm, PepMap RSLC C18 column, 2 μm, 100 A| ). The peptides were eluted from the column at a constant 8low rate of 300 nl/min with a linear gradient from 2% buffer to 5% in 5 min, from 5% B to 35% buffer B in 125 min, and then to 98% buffer B within 2 min. The column was then washed with 98% buffer B for 20 min. Two blanks were run between each sample to reduce carry-over. The column was kept at a constant temperature of 50 °C. The Q-exactive plus was operated in data dependent positive ionization mode. The source voltage was set to 2.30 kV and the capillary temperature was 250 °C.