values of c † calculated from the short-chain simulations are well below this overlap concentration (≈25 g/L for 30mers, assuming the size is the radius of gyration in a good solvent, and higher for shorter chains), though are approaching the estimated overlap concentration for 180mers (≈6 g/L, calculated using the scaling of R g with N obtained from the shorter chains in good solvent). This calculated overlap concentration is, however, a lower bound on the value, which will be higher in poor solvents where single chains are more collapsed, so the estimated values of c † for 180mers are still expected to be reasonable.