Figures and show the FTIR spectrum of hydroxyapatite nanoparticles (calcined and uncalcined samples), Euclea natalensis extracts root extract, and hydroxyapatite nanoparticles with artemisinin. The absorption peaks in the range between 560 to 606 cm -1 and 1045 cm -1 are ascribed to the ʋ4 bending mode and ʋ3 bending mode of PO4 3-, respectively . For the uncalcined samples, the peak at 1045 cm -1 shows greater length, while the calcined samples show greater clarity of peak formation. The characteristic double IR peaks around 1427 to 1475 cm -1 and the peak at 873 cm -1 are attributed to CO3 2-. The first one is the ʋ3 asymmetric stretching mode , with a shape not clearly defined for the uncalcined samples containing the template. The second one is the vibrational frequency of carbonate ions substituted at the phosphate site in the apatite , confirming the formation of B-type apatite . The absorption peak at 1647 cm -1 is due to OH -ions, indicating the presence of hydroxyapatite . The band at 3440 cm -1 represents adsorbed water, which decreases in intensity when the samples are calcined, showing less intensity for H5-C, H6-C-EN, H9-C-EN, and H10-C-EN .