To gain deeper insights into the temperature gradients of the phase separating systems, we computed the lateral distribution of temperature in the membrane and its difference between DLiPC and DPPC lipids (∆T ) with an in-house Python script using the MDAnalysis library. The kinetic energy of the lipids was calculated through E k = mv 2 /2, where v is the velocity and m is the mass of the particle. According to the equipartition theorem, the temperature was then obtained as