We first sought to develop abiotic TFs based on the DNA binding domain of Max TF. Max plays a key role in regulating gene expression by forming transcriptionally active (Myc/Max) and inactive (Mad/Max) protein dimers through its partner selection mechanisms. In addition, Max can self-dimerize to form a homodimeric TF complex (Max/Max) that binds to the canonical E-Box DNA sequence (Figure ).