When replacing DLiPC with the more saturated DOPC, phase separation becomes substantially less clear, with more mixing between DOPC and DPPC as well as a decrease in the enrichment of cholesterol-DPPC contacts (Figure ). No clear phase separation is observed at either 290 or 300 K. Experimental data points out that a single uniform phase should be obtained for DPPC:DOPC:CHOL at 300 K 116,123 , but coexistence of Lo and Ld phases should be observed at 290 K 116,123 . It is not clear how much our cholesterol model is impacting phase separation in this ternary model and whether there is much room for improvement, especially since the underlying DOPC:DPPC and POPC:DPPC binary mixtures also fail to show correct phase separation with the current Martini 3 lipid model 124 . If we compare the specific contact fractions calculated from the both Martini 3 and 2 systems (Figure i,j), we already observe a small improvement in the enrichment of DPPC-DPPC and DPPC-CHOL contacts with the newer model. These results hint at a slightly improved propensity to phase separate. Further developments focused on the lipid models will likely fix these ternary models, by improving the underlying DPPC:DOPC binary mixtures 124 . Moreover, it is worth noting that these results were obtained without the temperature differences arising from LINCS convergence and influencing the degree of phase separation . Indeed, the Martini 2 model phase separation was in part due to these issues, while our model can achieve similar levels of separation without this artifact.