Finally, to investigate how the constraint might affect the secondary structure of the peptide in the presence of Aurora-A, we employed MD analysis (Fig. , Fig. ). We based our analyses on the reported X-ray structure of the N-Myc/Aurora-A complex (PDB:5G1X), where the sequence of N-Myc was extended at the C-terminus to include either the natural residues 89-94 or the maleimide constrained G85C/N89C fragment.