However, studies focusing on polyphenols are scarce. For example, untargeted analysis of shiitake mushrooms revealed 17 metabolites that included phenolic acids, e.g. gallic acid or syringic acid, flavones, e.g. apigenin, and catechins . Some of the annotated polyphenols were also detected in our study, e.g. gallic acid, but others, e.g. catechin, were not detected even with the targeted method, which can be attributed to the limited identification confidence of previous work or to differences that arise from various growing conditions. For example, the fungal samples included here were cultivated, therefore their polyphenol profile may differ to those of wild mushrooms. 11