We demonstrated the timing-controlled division dynamics of DNA droplets using a timedelay circuit. We developed the reaction-diffusion model and numerically investigated the strategy to control the division timing by controlling the cleavage rate of L † AB. Using this strategy, we experimentally demonstrated timing control of the division of an A:Bdroplet by tuning the time-delay circuit parameters. We realized the pathway control of the C•A•B-droplet division by changing the order of two types of linker DNA cleavage based on the time-delay circuit. Finally, we employed the pathway control of the C•A•Bdroplet division for molecular computation and achieved a comparator of miRNA concentrations, which may be applied to a diagnosis based on the concentration difference of expressed miRNAs.