Sequence search was carried out in the Database of Essential Genes (DEG) . Out of 453 records, 44 were found to be unique hypothetical proteins encoded by these essential genes. These 44 hypothetical proteins were considered essential hypothetical proteins (EHPs) and retrieved from the UniProt () database in the FASTA format along with their UniProt ID for further analysis. Physicochemical properties such as molecular weight (Mw), theoretical isoelectric point (pI), grand average of hydropathicity (GRAVY), aliphatic, and instability index were computed for the EHPs using ExPASy's ProtParam server () .