To further understand the process of milestoning employed in the SEEKR2 framework, let us consider a system exhibiting a simple Muller potential, where an energy barrier exists between the two equilibrium states, A and B (Figure ). Let us define a CV as a distance variable that approximately describes the transition from state A to state B (Figure ). Anchor points are designated on the distance variable, constituting a one-dimensional Voronoi tesselation (Figure ).