However, it can also be seen that above +0.6 V vs. Ag/AgCl the fluorescent signal sharply drops, whilst at the same potential there is a rise in current. Clearly, some process above this potential is quenching the fluorescence, most likely further oxidation of the resorufin compound to resazurin. The quenching process needs to be avoided when the reporting electrode is held under galvanostatic control or applied in the bipolar setup for conversion of faradaic current to fluorescence. Therefore, the current passed in the detection cell must not exceed the limiting current of AR oxidation in the reporting cell. This could be achieved by either using a larger electrode in the reporting cell or an increased AR concentration to make sure that its oxidation is not the limiting process. In reality, these measures are limited by competing reactions at AR concentrations higher than 50 µM and by excessive charge consumption during the double layer charging of a large reporting electrode. Hence, to avoid the loss of faradaic charge towards charging of the electrical double layer, it is practical to use electrodes which are similar in size to compensate for charging currents and to ensure that the currents measured in the detection cell do not breach an upper threshold.