whereas BONCAT is based on the incorporation of labeled amino acids tags such as lhomopropargylglycine (HPG) and l-azidohomoalanine (AHA) . Although BONCAT is often combined with high-resolution microscopy and spectroscopy , the combination with mass spectrometric analyses was recently shown in studying the replication of phages during microbephage interactions , and the identification of effectors on bacterial pathogen infection . Thus, response mechanisms on various microbial effectors and the resulting physiological mechanisms can be identified with both methods. Bottlenecks such as restricted use of single labeled substrates in Protein-SIP and possible growth inhibitions by reactive substrate analogs must be considered and tested beforehand.