By adding BCNTPP or BCNHalo to cells incubated with MBTPA-Tz, strong turn-on emission was observed at the mitochondria and lipid droplets, respectively. Here, excellent colocalization signals alongside commercially available organelle-staining dyes (MitoTracker™ Deep Red FM and BODIPY 493/503) were observed, with Pearson correlation coefficients up to 0.95 and 0.84 (Figure ), respectively. However, when BCNDSPE was added, no turn-on emission was observed at the cell membrane. This could likely be attributed to BCNDSPE being initially internalized into the cell and taking some time to be expressed on the cell surface. To address this problem, BCNDSPE was added first and allowed to incubate for 24 hours, followed by a thorough washing of the cells and the subsequent addition of MBTPA-Tz. As a result, strong turn-on emission was observed at the cellular membrane (Figure ), which was further confirmed by colocalized signals using a commercially available membrane tracker (CellMask™ Plasma Membrane Stains) with a Pearson correlation coefficient of up to 0.93.