Panels G -I featured a pushes-per-bin value of 4, while panels A -F used a value of 3. The yellow star on panel I indicates the final tune settings we used for the SA, CRP, and TRAP experiments discussed below, except a pusher-per-bin value 0f 3 was used. Similar results could be obtained with the tune settings present in F -H. While it is important to consider different instrument configurations and detection strategies when comparing data acquired on different instruments, the Wysocki group has routinely shown that SID results are instrument agnostic, and that standard proteins should be used for benchmarking new SID installations. It is apparent when comparing the conditions in Figures and that what is optimal for ubiquitin (Figure ) is not ideal for CRP (Figure ). Because CRP is larger and features more non-covalent interactions than Ubq, the conditions that elongate Ubq do not have the same effect on CRP. This contrast highlights the importance of tuning an instrument for the analytes of interest and that no single tuning condition will preserve native-like structures for all proteins. Figure . A -I) SID bar chart for 765 eV SID of CRP 17+ pentamer. The letter acronyms are the same as in Figure , with the input value listed directly after the acronym. In the case of the helium cell (He) the entrance, cell bias, and exit are listed from left to right. The yellow star on panel I indicates the tuning we used for our experiments, though similar results could be obtained with conditions listed in panels F-H as well. Panel E also appears to give optimal SID results, but the high m/z portion was absent from spectra and the mobiligrams, which is not desirable. Panels F and G have the same tuning parameters, but the pushes-per-bin was increased from 3 for panels A -F, to 4 for panels G -I. The terms 1mer, 2mer, etc. were used in place of monomer, dimer, etc. due to space constraints within the figure panels.