20 Typha and 10 Pontederia crassipes were obtained from a local plant shop. The Typha was placed in a 1.5 L water tank filled with 1.2 L of water as their habitat to grow and live in. A 20 cm x 30 cm styrofoam lid with 20 holes of a diameter of 1.5 cm was made in order to put the Typha in the 1.5 L water tank while maintaining upright posture and not collapsing, ensuring the roots are sufficiently hydrated and are able to grow. The Pontederia crassipes was placed individually in a 5.0 L tank filled with enough water to cover the roots while maintaining the macrophyte in an upright position. Both containers received lighting from a LED lightbulb during the day for 8 hours to simulate sunlight so the macrophytes can perform photosynthesis and grow. The contaminated water is taken from a natural wastewater sample taken from the Drunken Moon Lake located in the National Taiwan University, which contains the contaminants Dissolved Oxygen (DO), pH, Ammonia nitrogen (NH₃), and Nitrate (NO₃). 5 L of the contaminated water is collected to be used in the experiment for each trial.