Here DS and DN are positioned in the 3' terminus of the DNA to study the dynamical properties of the UBPincorporated DNA in solution employing AMBER force fields for 1 µs for three replicates. It has been observed that the UBP in a terminal position is more flexible in nature as observed from the RMSF values (Figure ). This flexibility leads to form frequent mis-paired and frayed structures of the UBP (Figure ). RMSD values also suggest fluctuating characteristics of UUD structures. Notably, in this case nWCF structures are found to be predominant when the UBP is in the terminal position. Here also conformational change has been observed through both intra and interstrand flipping. From the EDA analysis, it has been noticed that the Coulomb interaction between adjacent cBPs are smaller compared with the internal UBP systems, indicating flexibility of the UBP also perturb the neighboring cBPs. It has been observed that DS and DN are stabilized through vdW interactions with associated EvdW=-6.5 kcal/mol indicating the interaction is significantly reduced than what is observed in nWCFP1 structures.