Experimental techniques used in chemical depolymerization experiments can be categorized into two groups: (i) separation-based, and (ii) bulk methods. The first group relies on physical separation of the soluble protein from the fibrils, and their subsequent quantification. Methods including (ultra) centrifugation or chromatography (HPLC) are often used, although they are time and sample consuming. Techniques from the second group rely on monitoring changes in specific features of fibrils or monomers as a function of increasing denaturant concentration, and include, for example, circular dichroism, light scattering, intrinsic fluorescence, or Thioflavin-T fluorescence . Spectroscopic techniques have the advantage of analyzing mixtures without the need for separation, which enables fast and high-throughput analysis. However, these methods provide relative measurements rather than absolute concentrations of the species involved which introduces some degree of uncertainty during analysis. Furthermore, they might require presence of specific fluorophores (intrinsic fluorescence) and can be limited by strong scattering (circular dichroism).