In conclusion, we have developed a novel method using a cold argon plasma for digestion of proteins and peptides. The key advantage of this approach over comparable methods is a substantial reduction in sample preparation time and effort and the elimination of reagents, including any use of enzymes. The use of cold plasmas in this way also maintains the spatial integrity of the macromolecules without the delocalisation caused by liquid-phase digestions. Initially, this method can be used to develop new databases of reporter ions for targeted or untargeted analysis -however, we intend to continue this research by elucidating the mechanisms of action, enabling rapid prediction of digestion products and, consequently, enabling structural elucidation of proteins from their plasma-exposed fragments, as well as the high-throughput imaging of proteins in condensed phase samples.