As a first test, we evaluated the average temperature difference between DLiPC and DPPC lipids, ∆T = T DLiPC -T DPPC . For the original cholesterol model, the insufficient convergence of the LINCS algorithm led to the development of significant temperature differences, as shown in Figure . With the least strict LINCS settings (lincs order=4) and largest time step (30 fs), the differences can reach ∆T = 56 K between the two lipid types (Figure ).