The film thickness increases from 0 to 36 nm upon application of F1 and decreases to 6.7 nm upon rinsing of the film, M1, indicating irreversible polymer grafting to the Si substrate, despite the lack of an observable nanostructure. The film thickness again increases to 36 nm for F2 and decreases to 7.6 nm for M2 indicating a second round of polymer grafting from F2. The increase in monolayer thickness from M1 to M2 indicates that the BCP molecules are able to interpenetrate the existing brush and graft to the surface. The incremental amount observed at M2, 0.9 nm, is less than that observed at M1, 6.7 nm, possibly due to the polymer chains needing to diffuse through the previously grafted monolayer, M1, to bind to the substrate, which could hinder the grafting process. The WCA data follows a cyclical trend between the BCP films and monolayer. The WCAs of the BCP films are approximately 75°. The quantitative mismatch between the WCAs of F1 and F2 is likely due to the non-uniform surface of the island features in F1 and F2, which could change the relative PS/PG(B-r-C) composition of the surface for the two samples. The WCAs of M1 and M2 on the Si substrates are close to 90°, which matches that of pure PS and indicates that PS wets the free interface in the monolayer and the PG(B-r-C) block wets the Si substrate. This is expected due to the (1) higher hydrophilicity of the PG(B-r-C) block compared to the PS block and ( ) grafting sites being located on the PG(B-r-C) block. Therefore, despite the lack of observable nanostructure in both the films and monolayers, we still observe irreversible grafting of the BCP to the substrate via the self-brushing mechanism.