The plant was identified by the botanist Retta Regassa, Department of Biology, Hawassa College of Teacher Education, and sent to the National Herbarium of Ethiopia and given voucher specimen number (Voucher no-MM364) for conformation and indeed it was Rubia cordifolia Linn. The fresh collected roots of Rubia cordifolia were thoroughly washed using distilled water to remove dirtiness, air dried in the shade and stored. The dried plant root bark were cut into small pieces, air-dried, and grounded into a fine powder using an electric grinder.