After elucidating the different reactivity of Tc_Cut2NV WCCG and LCC WCCG for the hydrolysis of model small molecules and model substrates, we applied the enzymes to the depolymerization of polyesters polymers. The enzymes developed were used to analyze an aliphatic, PES1 (DDA-EG, 2-1, Mw= 17 kDa), as well as the aromatic PES3 (TPA-EG, 3-1, Mw=132 kDa) and PES4 (IPA-NG, 3-6, Mw=14 kDa), homo-polyester synthetic models (Fig. ). PES3 and PES4, which exhibit significant differences in molecular weight, were used to assess the influence of this parameter on depolymerization. The polymer samples used in this study were solid materials in different forms such as powder/ granules (PES 1,2,4), or blocks (PES3). The polymers were hydrolyzed as solid dispersions in water. However, for most of the polymers, the reaction temperature of 71 °C exceeded their glass transition temperature (Tg), except for PES3 (see Table ).