Single-site glycan mutant and WT SARS-CoV-2 pseudoviruses were treated with serial dilutions of CV-N and evaluated for infectivity as previously described. A subset of glycans in the S1 subunit showed a reduced sensitivity to CV-N activity, indicating a potential role in CV-Nmediated pseudovirus inhibition: N61, N122, N331, N343, N603, N657 (Table ). This experiment was also performed with His-tagged GRFT against the same panel of single glycan attachment-site mutant pseudoviruses, identifying several glycan-bearing residues overlapping with the CV-N subset, although not identical: N61, N165, N331, N343, N603, N616, N657 (shared required residues underlined).