Lardeux et al. showed that in HILIC low temperatures are favorable for achieving diastereomer separation, whilst higher temperatures could disrupt ON conformations leading to singular, sharp peaks. To assess the effect of column temperature on the separation of PS ONs in IP-HILIC, column temperatures of 5, 20, 45, 60, and 80 °C were tested (Fig. ; chromatograms in Fig. ). The TEA concentration and pH were kept at 25 mM and 7, respectively.