Using this MLP, we also computed the temperature dependence for both activation and reaction energy in order to compare the MLP-US method to the static methods (Fig. and). For this DA reaction, MLP-US gives activation and reaction free energies comparable to the static methods, with a linear dependence on temperature. While no experimental data is available for the free energy of activation of this reaction in the gas phase, the comparison of the different static approaches to our MLP-US values suggest an estimate error of ≈ 3 kcal mol -1 for the free energy contributions. When comparing free energies of reaction (Fig. ), a larger deviation and larger error bars are observed at low temperature. This is likely due to the insufficient sampling; thus this point is excluded from the linear fit.