The program gmx solvate was used to create cubic solvent boxes with a side length of 7 nm. Water coordinates were taken from the spc216.gro file contained in GROMACS. Chloroform and DMSO coordinates were created from a single copy of the molecule, while adjusting the box size to create roughly the correct density (0.52 nm in each direction for chloroform and 0.58 nm × 0.45 nm × 0.45 nm for DMSO).