UV spectrophotometer was used to analyze the samples. About 3 mL of blank sample was taken and analyzed using UV visible spectrophotometer and was confirmed to be BPA free. This means that if BPA ensue later in the analysis, it would be ascribed to migration of BPA from the PC bottles examined in this study. Also 3 mL of aliquots was taken from each control sample daily and analyzed using UV-visible spectrophotometer for ten consecutive days. For treated samples, 3 mL of the aliquots was taken from each sample and analyzed using UV visible spectrophotometer after been heated in the ovens for four hours daily and cooled in the refrigerator. The samples were kept in the refrigerator until the next day after analysis. This procedure was repeated for ten successive days and the concentrations of the analyte in the various samples were determined from the absorbance data by using the concentration equations generated from the calibration curves.