Eight to ten-week-old BALB/c female mice were used for these experiments. All experimental procedures were approved by the local ethics committee in Bydgoszcz (Approval No. 49/2016). Before experiments, mice were randomized and divided into groups comprising six individuals. The mice were anaesthetized with inhaled 2-5% isoflurane. The skin on the dorsum was shaved and disinfected. After the skin was folded and raised cranially and caudally along the spine, the mouse was placed in a lateral position, and the folded skin was pierced with a 6.0 mm biopsy punch to make two through-andthrough symmetrical wounds. Next, 10 µL of 1% peptide hybrid hydrogel was applied onto each wound. Control mice were treated with saline. The wounds were covered with a transparent Tegaderm TM dressing that was fastened with an adhesive plaster wrapped around the animal's torso. The peptide hybrids were applied daily to the wounds for five consecutive days. The dressings were replaced daily in the first five days of the experiment and every second day until the end of the second week of the experiment. To measure the wound areas the wounds were photographed with a ruler placed next to the injury site. Wound areas were calculated using the computer-assisted image analysis program ImageJ .