After receiving the 1,028 compounds, we conducted two replicate screens following the same protocol described above for the MLPCN library. We excluded four compounds because they could not be dissolved and plated. We analyzed the % inhibition distributions for the 1,024 screened compounds for each replicate individually to determine the hit criteria (Figure ). For a large random screen, we could set the % inhibition cutoff some standard deviations above the mean. Thus, we could use the same MLPCN threshold of 35% inhibition.