Surface pressure measurements are conducted using complementary techniques: the Langmuir-Wilhelmy balance method, which provides direct force measurements and enables simultaneous microscopy, and the pendant drop technique, which offers advantages for high-temperature studies despite increased sensitivity to vibration and evaporation effects. Inherent strengths and limitations of each method were carefully considered in data analysis and interpretation. The pendant drop apparatus determines surface tension by analyzing the droplet's shape using the Young-Laplace equation. The accuracy of surface tension measurements directly hinges on the precise fitting of the droplet shape. Particularly at low surface tensions, irregularities in droplet shape can result in inaccurate measurements. Pendent drop measurements are highly sensitive to environmental conditions, making experiments challenging at high temperatures due to droplet evaporation. On the other hand, the Langmuir trough measures surface tension by gauging forces with a Wilhelmy plate. Temperature regulation with precision of ±0.5°C throughout the experimental measurements is essential for obtaining reproducible isotherms, as even small thermal fluctuations can significantly impact phase transitions and molecular reorganization in the monolayer. However, the Langmuir trough apparatus is prone to leakage issues at low surface tensions, potentially causing premature monolayer collapse. Nevertheless, using the Langmuir trough offers the advantage of simultaneous in-situ fluorescent imaging, providing additional insights into lipid configuration. Compression rate significantly influences isotherm characteristics through its impact on molecular reorganization kinetics. Our systematic investigation of compression speeds (5-20 mm/min) revealed that rates exceeding 15 mm/min led to artificial smoothing of phase transitions and premature collapse, while rates below 8 mm/min increased susceptibility to environmental perturbations and film loss. The optimal rate of 10 mm/min was selected based on reproducibility of phase transition plateaus (±0.5 mN/m) and collapse pressures (±1 mN/m). Quasi-equilibrium compression conditions, achieved at rates ≤10 mm/min, allow sufficient time for local molecular reorganization and domain formation, as verified through real-time Brewster angle microscopy showing stable domain structures. This equilibration is particularly critical during the LE-LC phase transition, where domain growth and coalescence processes require timescales on the order of minutes. This results in more accurate and reproducible isotherms, reflecting the true phase transitions and properties of the monolayer. Rapid compression rates artificially smooth the isotherm features by preventing the monolayer from achieving local equilibrium, thereby masking important phase transitions and potentially leading to premature collapse through non-uniform stress distribution. For both the tensiometers and Langmuir-troughs, the method of depositing the DPPC molecules at the interface can have an impact on the isotherm. The initial monolayer structure is highly sensitive to multiple experimental parameters: the choice of spreading solvent (which affects molecule-substrate interactions), the volume and distribution of deposited droplets (which influence local concentration gradients), and the solvent evaporation period (which determines molecular reorganization during film formation). For instance, insufficient waiting time for the solvent to dry before beginning the compression can cause the initial lift off pressure to vary between experimentalists.