Method parameters were as follows: a scan range of 2,000-8,000 m/z, an AGC target of 3 x 10 6 , a max. injecNon Nme of 200 ms, 10 microscans and a CID of 100 eV. SCX analysis was carried out using a 2.0 × 50 mm, 3 μm ProPac 3R SCX column (Thermo ScienNfic, Sunnyvale, CA, USA). Mobile phase A was 25 mM ammonium bicarbonate and 30 mM aceNc acid in water (pH 5.3) and B 10 mM ammonium hydroxide in water (pH 10.9). The LC gradient was as follows: 5% B to 75% B in 15 min, 75% B to 100 % B in 0.1 minutes followed by a hold from 15.1 to 20.0 min, 100% B to 5% B in 0.1 min and equilibraNon for 14.9 minutes. The column temperature and flow rate were maintained at 25 °C and 200 μL/min, respecNvely, throughout the chromatographic run. Analysis was performed in HMR mode using a resoluNon se}ng of 35,000 (at m/z 200). The tune parameters were set to the following: a sheath gas flow rate of 20 au, an auxiliary gas flow rate of 10 au, a spray voltage of 3.8 kV, a transfer capillary temperature of 320 °C, a vaporizer temperature of 225 °C and a RF lens se}ng of 200%. Method parameters were as follows: a scan range of 2,000-7,500 m/z, an AGC target of 3 x 10 6 , a max. injecNon Nme of 100 ms, 10 microscans and in source CID of 100 eV.