were then co-incubated with preformed PFKDOPA fibrils solutions at various concentrations (0, 0.45 mM, 1 mM, 2 mM, 3 mM, 4 mM, and 5 mM), prepared in Hepes buffer (50 mM, pH 7.4) supplemented with 0.6M KCl. The samples were incubated for 2h at room temperature. 100 µL of the preformed fibrils solutions were co-incubated with 50 µg beads. Following this, 50 µL of the supernatant solution was collected and transferred to clear UV-Star 96-well plates (Greiner flatbottom), and the absorbance was at λ=258 nm using Biotek Synergy H1 plate reader (Biotek, Winooski, VT, USA).