Compounds 23-26 did not affect HFF viability at 0.1 µM. However, uracil and adenine derivatives 23 and 26 presented a higher toxicity to the host cells at 1 µM (76% for both). Compared to its congeners, thymine functionalized compound 24 had a stronger antiparasitic effect at 0.1 µM, while cytosine derivative 25 had a minimal impact on parasite proliferation even at 1 µM. Nevertheless, compounds 23, 24 and 26, bearing uracil, thymine and adenine, almost abolished parasite proliferation when applied at 1 µM. Compared to the diruthenium azide precursor 10, nucleobase click conjugates 23-26 applied at 1 µM affected HFF to a lower degree, but were not active against the parasite at 0.1 µM.