Plotting sample composition by AA class for samples collected in August 2019 (figure ) demonstrated a high percentage of macrolides in hospital effluent (43%) compared to community wastewater influent (12%). Interestingly a higher percentage composition of sulphonamides was observed for community wastewater (37%) versus hospital effluent (15%). A similar trend was noted for antiretrovirals, with AAs of this class making up 7% of community wastewater, compared to <1% of hospital effluent. By comparing percentage catchment prescription versus hospital prescriptions in August 2019 (figure ), the percentage of sulphonamides prescribed in hospitals was significantly higher than in the wider catchment (57% versus 33% respectively). Prescriptions of macrolides however were lower in hospital prescribing (2%) in comparison to community prescribing (7%). Why the same composition patterns were not reflected in wastewater is likely due to various reasons, including metabolism and degradation of AAs in wastewater, as well as patient compliance.