We rescreened our 10K set of primary hits under these revised ADP-Glo TM conditions and observed a marked reduction in confirmed hits (Figure ) from 7.4K for the original analysis to 1.6K under the new assay format (5σ threshold, 12.5% median inhibition). Again, applying cheminformatics approaches to select compounds for concentration-response, we identified a diverse set of 477 compounds for profiling from freshly purchased or retrieved powder stocks.