The obstacles of the application enzymes in industry are stability, long term storage, reusability, and recovery. Immobilization of enzymes removes these disadvantages and enables the application. Taguchi method to optimize the immobilization of lipase A from Candida antartica(CALA) onto halloysite nanotubes (Hal) for p-nitrophenyl butyrate hydrolysis. In this study, CALA-Hal displays better stability at 50-90 °C and higher catalytic activity at pH 9 compared to original lipase A.