High throughput X-ray crystallography requires that protein crystals must diffract at a good resolution and resist fracture during soaking. Previous efforts demonstrated that crude reaction mixtures can be prepared and soaked onto protein crystals and that reaction products can be resolved. Here, crude reaction mixture preparation was also included in our robotic framework where the products were concentrated in the organic phase and solvent exchanged. Due to the intrinsic affinity of bromodomains for DMSO (dimethyl sulfoxide) and/ or DMA these solvents were replaced with ethylene glycol.