Then the Rotatory evaporator apparatus is employed to carry out the evaporation of the solvent mixture under reduced pressure applied at 45•C for the duration of 15 minutes. This evaporates the organic solvent mixture which leaves behind a thin lipid film on the flask surface. Rotary evaporation is quite efficient for removing organic solvents with the help of vacuum pump forming uniform thin film. It also ensures minimal degradation of heat-sensitive Curcumin. However, it does require precise control to avoid overheating that could degrade Curcumin. Lastly the previously obtained thin film of Curcumin loaded lipid nanoparticle is hydrated for an hour using pH 7.4 Phosphate buffer solution to form the nanoparticles. The phosphate buffer provides a biocompatible medium and hydration allows the lipid layers to reorganize into nanoparticles. However, prolonged hydration may also lead to aggregation of nanoparticles. The hydrated lipid film is subjected to sonication and is filtered by 0.45𝜇m membrane filter. Then the well appropriated drug loaded Lipid nanoparticles are formed and stored at 4•C. The sonication reduces particle size and improves uniformity, it also increases the encapsulation efficiency of Curcumin within the lipid matrix. However, prolonged sonication can cause heat generation which can potentially degrade the Curcumin. It also requires optimization to archive the desired size of nanoparticles without aggregation . Schematic diagram for preparation of curcumin nanoparticle by Thin Film Hydration Method is shown in figure .