The OMPLA and ClC homodimer systems were simulated using the CHARMM36m force field, 45 a well-tested force field for phospholipids and transmembrane proteins. The OMPLA and ClC homodimer systems were set up in a cubic periodic box using CHARMM-GUI. The membrane of the OMPLA homodimer system was created as a symmetric 1:1 with a real-space cutoff of 1.2 nm. The systems were first minimized and equilibrated in a series of small equilibrations, with gradually weaker restraints on protein and lipids (generic CHARMM-GUI settings). The system was then equilibrated for 10 ns with only restraints on the protein backbone heavy atoms. Production runs were 1 µs with 3 repeats for each system. Frames were saved every 100 ps.