Next, in order to investigate the important amino-acids, we estimated the amino-acids of MATE1 transporter which interact with inhibitors through SIFts. The result of this analysis is shown in Figure where it can be seen that several amino-acids emerged frequently as contacted aminoacids, out of a total set of 570 amino acids present. The number of amino-acids that interact with inhibitors was 58, and those with non-inhibitors was 55. Interestingly 50 amino-acids were overlapped (Figure ). When we focused on amino-acids that interact with more than 10 inhibitors, we can find that Gln49, Ph53, Asn82, Trp274, Tyr277, Glu278, Tyr299, Ile303, and Tyr306 are involved in forming interactions (Figure ). Most of these amino-acids are related to the contact with non-inhibitors. Although only Glu278 had the larger number of contact in inhibitors than non-inhibitors, the difference of it was not so large (63 inhibitors and 41 noninhibitors). Hence, we can conclude that the path of MATE1 for the inhibitors and non-inhibitors are mostly the same.