Two P. aeruginosa bacterial strains were used in this study: the PAO1 reference strain is a clinical isolate from a human wound, and the PPF-1 strain is a DUWL isolate, which is the focus of this study. From a single colony of bacteria grown overnight on tryptic soy agar (TSA, Wisent, St-Bruno, Canada), a bacteria pre-culture (room temperature, 200 revolutions per minutes, 25 ±5 h) was prepared in 3 mL of 1/10 diluted lysogeny broth (LB 1/10) (EMD, Mississauga, Canada) and used to inoculate the system.