An interesting study was performed by Borges et al. . The authors investigated the effect of the natural limonoid gedunin on different TLRs (2, 3, and 4) activation. They performed in vitro, in vivo and in silico studies. The experimental results confirmed that gedunin is able to impair inflammasome activation, and cytokine production and induce anti-inflammatory factors in macrophages. The in silico studies revealed that the investigated compound can efficiently bind to the TLR2, TLR3, MD2 protein of TLR4 and also to the caspase-1, making gedunin considered a multitarget compound. For both TLR2 and TLR4, gedunin bound within the known ligand binding site, while for TLR3 two distinct binding sites were predicted. The authors pointed out that one of the predicted regions for TLR3 is involved in the dimerisation of TLR3 and is considered the dsRNA binding site, thus it might be the most prominent. Still, as pointed out by the authors, further biochemical assays are required to confirm gedunin binding.