Proteins with brackets of this size and difficulty have been achieved by leading labs in the field , but currently require intensive time and resources to produce. Yield losses at far-downstream steps can be devastating, so proper planning is vital. Thus, syntheses in this category could benefit greatly from computational assistance as well as ligation-and solubilityenhancing tools described previously (although these chemical tools have yet to be demonstrated in context of a large protein ≥300 aa).