After 24 hours, the cells were pelleted via centrifugation (~3800 RPM, 4 °C) for downstream processing. Cell pellets were lysed with 10 mL B-PER (ThermoFisher) containing 1 mM PMSF protease inhibitor. Lysates were then clarified by centrifugation (14,000 x g, 20 min, 4 °C) and the supernatant collected for purification via IMAC. The supernatant from clarified cell lysate was applied to a gravity flow column containing HisPur Cobalt Resin (Thermo Scientific), washed with PBS, and then eluted with increasing concentrations of imidazole in PBS.