Next, we tested the methods on the "Standard" synthetic ensembles generated from the bound vanish stages. The fitted parameters used to generate the synthetic ensembles were fairly diverse, with the variance of the mean estimates, max lag index (for the initial convex sequence estimate of the autocovariance series), and pre-exponential factors for the "slow" exponential fits varying over an order of magnitude (Table ). The half-lives of the "slow" exponential fits varied from 0.3 to 1.6 ns. Further details of the parameter fitting and synthetic data generation are given in Section S2. a Total variance refers to the total variance of the mean, obtained by summing the autocovariance series from -max lag index to + max lag index, where the series and maximum lag indices were estimated according to Geyer's initial convex sequence rules.