Recent studies using liposome nanoparticles functionalised with an outer polyethylene glycol (PEG) molecular layer have shown that protein corona formation is governed by additional parameters beyond nanoparticle surface charge . In addition, the conditions used for nanoparticle incubation with protein containing media, their recovery procedure and the analytical method need to be considered for the comprehensive assessment of novel nanoparticle prototypes for biomedical applications and to enable comparability across multiple studies.