Herein we report on key advances in the field of supramolecular protection using this macrocycle. The first new concept that we explored was the possibility of regenerating the molecular container in situ after a supramolecular protection/monofunctionalization sequence. This would considerably increase the synthetic viability of the supramolecular approach as a functionalized product could then be accumulated through a one-pot cyclic process involving a small amount of molecular container. Another aim was to evaluate the synthesis of products, which are difficult to obtain via classical synthetic routes, through the one-pot reaction of mixtures of difunctional substrates monoprotected by a molecular container. Finally, we also explored the supramolecular approach with complex polyfunctional substrates for the development of regio-and/or iteroselective reactions.