Using the phosphorus density values observed in the engineered PST+ strain, we updated our process model and techno-economic analysis to evaluate the impact of our cyanobacteria strain optimization (see Figure ). Using the PST+ strain improves process performance across all considered metrics when compared to wild-type. The increase in P density resulted in a reduced need for cyanobacteria biomass, which in turn reduced the process throughput and the TAC by 53%, as the back-end (cultivation and recovery) is much smaller when compared to the baseline process. The PRC decreased by 59% to 48 USD/kg P, which is 35% lower than the socioeconomic cost of P runoff (75 USD/kg P). The energy demand decreased by approximately 68%. Makeup water is no longer required, as the manure feed contains enough water to make up for the losses in the product recovery section. Additionally, the land required for the b-PBRs was reduced by 88% (to 3 acres), similar to the footprint required for manure storage on a 1000 cow farm. Increasing phosphorus density dramatically reduces the amount of cyanobacteria biomass required to capture P by a factor of 8.5. This results in major changes to process design and operation. First, the AD system now produces more than enough CO2 to meet the carbon needs of the cyanobacteria, making combustion of the CH4 for CO2 generation unnecessary. As a result, all the generated methane can be exported off-site, as is the norm at existing AD facilities. This allows us to reduce the capital cost by removing the electricity generator from the process (shown in Supp Fig ) and increase the revenue generated from the biogas. Second, the reduction in water demand requires that the percentage of material purged be increased from 5% to 18% to close the material balance. However, this still represents a reduction in the purge flowrate of 57%.