Characterisation and quantitation of glycan signal are common steps in the glycoanalytical data analysis workflow, yielding information about the abundance and identity of the glycan signals detected. GlycoWorkbench is a characterisation-oriented software tool, useful for matching MS1 and MS2 signals to drawn structures, or those found in databases. Like GlycoMod, it remains widely used demonstrating its enduring value for composition and structure characterisation . Orthogonal to the characterisation approach of GlycoWorkbench, Skyline is a quantitation tool that uses predefined molecule transitions to generate EICs, measuring glycan signal in LC-MS data and providing scores based on signal relationships. A useful score used in this work is the isotopic dot product (idotp) which describes how closely the isotopic distribution of an observed precursor isotopic envelope matches the theoretical abundances of a given chemical formula .