Next, we explored the potential versatility of our DNA enzyme nanoswimmers by using another enzyme as a source of self-propulsion (Figure ). For this purpose, we chose catalase, the most commonly used enzymatic drive for self-propelled micro-and nanomotors. Catalase is capable of converting hydrogen peroxide (H2O2) into water and oxygen (O2), which can lead to self-propulsion by i) the generation of bubbles or ii) a diffusiophoretic motion mechanism. We conjugated catalase to ssDNA strands using a reaction similar to that used to prepare urease-DNA conjugates (see the experimental section in the supporting information and Figure ). We then functionalized the DNA nanostructures using 30% of the tiles with anchor strand (as in the case of DNA urease nanoswimmers) (Figure ). We detected the presence of catalase (Cy3) on the surface of the DNA nanostructures (Cy5) using fluorescence microscopy images (Figure ). Comparable values for average length (Cy5 DNA structure: 2.8 ± 0.3; Cy3 catalase: 2.3 ± 0.2 µm) and count (Cy5 DNA structure: 4.4 ± 0.2; Cy3 catalase: 4.4 ± 0.4