CRISPR technology, originally harnessed for gene editing, has rapidly evolved into a powerful tool for disease diagnosis. Its ability to detect specific genetic sequences is invaluable in identifying infectious diseases, genetic disorders, and even cancers. Although quantitative polymerase chain reaction (qPCR)-based nucleic acid detection is a gold standard method in routine clinical practice, it relies on optimizing numerous processes, such as DNA or RNA extraction, (RNA integrity control and cDNA synthesis in the case of RNA), primer design, amplicon detection, and data normalization. Isothermal amplification and next-generation sequencing (NGS) are also used in routine clinical diagnostics. For comparisons between the three most prevalent molecular diagnostic methods please see Table in supporting information.