In proteomics, although sequence database searching is still the method of choice in datadependent acquisition (DDA) experiments, spectral library searching offers notable advantages. First, they enable faster identification due to a smaller search space of observable peptides. Second, these libraries can increase the specificity and sensitivity of peptide identification tools by using known fragment ion intensities rather than solely relying on the calculated mass-to-charge ratios of the expected fragment ions. On the other hand, in data-independent acquisition (DIA) experiments, where the fragmentation of multiple precursors simultaneously leads to convoluted and hard-to-interpret spectra, spectral libraries play a crucial role in data analysis, in particular in peptide-centric approaches that seek to monitor anticipated fragment ions co-eluting over time .