The rate and degree of the denaturation process depends on the following interrelated parameters: temperature, structure and size of DNA, the composition of surrounding media (solvents , buffers , pH, salts , 17 , 18 ) . Many theoretical and experimental works have been published to describe factors related to DNA denaturation. However, the precise mechanism of DNA denaturation is still poorly understood and has continued to attract the attention of researchers. In this review, we call the reviewed works where significant attention has been related to the creation of the theory the "theoretical works." We call the studies where the primary emphasis was placed on the experiment the "experimental works."