Dopamine (DA) is a neurotransmitter that plays an important role in the cardiovascular and central nervous systems. Therefore, high DA levels indicate cardiotoxicity leading to rapid heartbeat, hypertension and heart failure. On the contrary, low DA levels in the central nervous system are seen as the main cause of various neurological diseases such as Parkinson's disease, schizophrenia, Alzheimer's disease, stress and depression . Therefore, it is clear that DA measurements are necessary to understand its biological functions and related biological processes/mechanisms. Currently, there is a growing interest in developing specific and low-cost biosensors that take advantage of the ease with which DA is oxidized on an electrode surface. In addition, the biosensors should be able to provide a sensitive response in the appropriate concentration range (0.01-1 μM for a healthy individual and in the nanomolar range for patients with Parkinson's disease) . In this study, sensitive detection of DA was performed with a dual ITO microchip that can amplify the signal based on redox cycling. The 3×3 mm working areas of two ITO microchips were designed to overlap with the help of double-sided tape, and a dual ITO microchip was created with a microchannel. The applicability of the dual ITO microchip as a biosensor was analyzed using different concentrations of DA solution.