In the context of cardiovascular conditions, oxidative stress plays a central role in the development of numerous conditions, such as atherosclerosis, myocardial infarction, and heart failure . The accumulation of ROS not only damages cardiac cells directly but also triggers a cascade of inflammatory responses . In cardiovascular disease conditions, ROS can be induced by various external and internal factors such as environmental toxins, radiation, inflammation, and certain medications like chemotherapeutic drugs . Among these, chemotherapy agents like doxorubicin (DOX) are well-known inducers of oxidative stress, especially in cardiac tissues . Although, DOX is a highly effective antitumor agent used for treating various malignancies; however, its clinical application is limited by its dose-dependent and cumulative cardiotoxicity, often called the "Red Demon" . DOX induces oxidative stress within cardiac cells by generating excessive ROS. This oxidative stress damages cellular components, leading to mitochondrial dysfunction and activation of apoptotic (programmed cell death) pathways. The resultant cell death contributes to the deterioration of cardiac function, which can manifest as acute heart failure . Consequently, the mortality rate following DOX therapy ranges from 10 to 38% after a single or cumulative dose of 5-25 mg/kg, and increases significantly to 50% within two years after chemotherapy . To overcome DOX-induced cardiotoxicity, researchers are exploring strategies to counter DOX-induced cardiotoxicity, including using antioxidants , nanotechnology , gene therapies , mitochondrial protection strategies , and adjusting chemotherapy regimens to reduce cardiac risk, thereby enhancing cardiac defenses. While these techniques offer valuable approaches to reducing cardiotoxicity, the detection of ROS in DOX-induced cardiotoxicity stands out as a particularly effective strategy. Detecting elevated ROS levels is an early warning system for oxidative stress, allowing clinicians to intervene before significant cardiac damage occurs . This proactive approach allows for timely adjustments in treatment, such as modifying dosages or initiating therapies. Detecting ROS levels also helps monitor the effectiveness of interventions, risk stratification, and the development of targeted therapies.