The results of this work underscore the potential of microflow-based approaches in discovery lipidomics. In our opinion, the presented configuration provided a robust alternative to more fragile nLC systems. Not only was the initial disparity in the injected material compared to conventional high-flow methods fully addressed, but there was also a clear net positive gain in the quantifiable signal. Significant improvement in the analytical coverage was also obtained, which is of special interest to researchers working with limited amount of biological material. Additionally, the µF/mnESI method generated a consistent dataset with higher information content and quality metrics comparable to established lipidomic pipelines.