The obtained results, illustrated in Figure , show that the assay setup tolerates even high DMSO concentrations up to 10% (v/v). Subsequently, we tested several fragments of intracellular CCR2 ligands (e.g. SD-24 (1) and 27) for their ability to compete with 14 for intracellular CCR2 binding (Figure ). As the four fragments that showed the highest CCR2 binding affinity harbour a biaryl sulfonamide substructure, our results highlight the importance of this substructure for intracellular CCR2 binding from a fragmentbased perspective.