The β2AR is a class A GPCR associated with the downstream regulation of L-type calcium channels and subsequent modulation of physiological responses such as relaxation of smooth muscle, bronchodilation, glycogenolysis, and gluconeogenesis. It is also involved in the regulation of the cell metabolism in skeletal muscle in response to noradrenaline and adrenaline . X-Ray crystallographic data from multiple sources show two clear cholesterol interaction sites between helices 1 and 4 as well as between helices 1 and 7 130,131 (Figure ). For convenience, we labeled this interaction site as binding site 1. Cholesterol interaction in this site is also observed in atomistic MD studies of β2AR . Our Martini 3 cholesterol model recovers binding at this interaction site with high residence time, in agreement with the existing references (Figure a, b, c).