To prepare artificial ISF, 2.5 mM CaCl2, 5.5 mM glucose, 10 mM HEPES, 3.5 mM KCl, 0.7 mM MgSO4, 123 mM NaCl, 1.5 mM NaH2PO4, and 7.4 mM saccharose were dissolved in Milli-Q water and the pH was adjusted to 7.0 using HCl solution . All chemicals were used without further modification and purification. Glucose detection in artificial ISF was conducted by ECWP110plus wireless potentiostat (Zensor R&D co., Ltd). The potentiostat device consists of a remoter and a connector. SANs/GOx-coated SPE was inserted into the connector, while the remoter was connected to the USB port of a computer. The connector collected the electric signal from SPE and transmitted the signal to computer via the remoter. Amperometric method was applied to analyze the signal from connector in EIWP110 software (Zensor R&D co., Ltd) on computer. The operation interface is displayed in Figure . As shown in Figure and B, the potential was set to -0.22V to obtain the response currents which were obtained to create the standard curve correlating current with glucose concentration in ISF. In Figure , the standard curve equation was entered into software for subsequent detection. The above method was also applied to continuous glucose detection in ISF. To perform continuous detection, the glucose ISF solution was added to the ISF solution to achieve a continuous increase in the ISF solution. During the continuous measurement, concentrations of glucose were 0.98, 5.98, 10.60 and 20.05 mM, respectively. After adding glucose solution in artificial ISF, the magnetic bead was constantly stirred in the ISF solution to quickly reach the target glucose concentration. After analysis by EIWP110 software, the amperometry curve was shown. The concentration would be calculated using the formula that has been entered and displayed on the software interface. Meanwhile, the results were sent wirelessly and displayed on the smartphone app, ECWP (Zensor R&D co., Ltd).