Initially, the mouse was given a retro-orbital injection of tumor targeting s775cRGD (10 nmol) and 3 hours later, an injection containing ICB (100 nmol) was delivered retro-orbitally. The living mouse was imaged using filter settings that separately visualized each fluorescent probe (i.e., 700 nm fluorescence channel for ICB and 800 nm fluorescence channel for s775cRGD). Shown in data that confirms these conclusions). In contrast, the s775cRGD cleared much more slowly from the mouse and significant amounts remained in the tumor after 24 hours. At that time point, the mouse was sacrificed, and the organs imaged to determine a final biodistribution (Figure ).