Considering the findings of Kumada et al. that RADA-16 hydrogels can support the growth of human ligament fibroblasts, we decided to evaluate the effect of the RADA derivatives tested in our work on the viability of human dermal fibroblasts. In Kumada's work, two short biologically active peptide motifs, namely a 2-unit RGD-binding sequence PRG (PRGDSGYRGDS) and a laminin cell adhesion motif PDS (PDSGR) were coupled with RADA16-I. They showed that the designed scaffolds could enhance proliferation and migration of human periodontal ligament fibroblasts and the production of collagen type I and III in cell culture. This indicates that they might be used to reconstruct periodontal tissue. The experiment was demanding to perform due to the cells sinking into the gel and being difficult to observe under a microscope. The most challenging part was to visualize cells on the RADA-IM scaffold. Our results showed that after 3 days of incubation on the scaffold, human fibroblasts remained viable and showed a characteristic spindle-shaped morphology. These results indicate that the designed hydrogels support the growth of human fibroblasts and may serve as scaffolds for these cells in wound healing.