Fluorescence Spectroscopy-Critical Micelle Concentration (CMC). CMC of triblock copolymers was determined using fluorescence probe pyrene. Pyrene solutions (24 µM, 5.0 mg/L in acetone) were added to glass vials and followed by acetone removal by a gentle stream of argon. Afterwards, various amounts of aqueous polymer stock solutions were added, and the solutions were diluted with water (Millipore) to yield a final pyrene concentration of 5×10 -7 M. The samples were shaken gently for 30 min and stored overnight at ambient temperature (≈20 °C) under the exclusion of light. Fluorescence measurements were performed in a FP-8300, Jasco from 360 nm to 400 nm (λex = 333 nm) at 25 °C with 10x10 mm fluorescence cuvettes.