Before the experiment, it was diluted with THF in a volumetric flask to get the final concentration of 1 mg•ml -1 . This was important to create stable films at the air/water interface of particles, which create relatively stable suspensions in water, i.e., subphase. PNIPAM molecules in pure water or pure THF are stable in an open (coil/extended) form due to solvent-polymer hydrogen bonds or van der Waals interactions with the solvent, respectively. Nonetheless, it was proven that at the specific range of molar fractions of water/THF mixtures and the temperature (0.05<𝑥 𝑇𝐻𝐹 <0.47, T=24°C) PNIPAM becomes insoluble (transition to globular form) due to composition fluctuation of the mixed solvent system. A similar phenomenon is known for other good solvent mixtures, e.g., PNIPAM in methanol-water co-solvent. The subphase was heated to 40 °C (± 2 °C) before the deposition. Both temperature and co-solvent caused the PSiFe to be in "closed" conformation, i.e., more hydrophobic and thus more prone to remain at the water surface.