Polymers are promising non-viral delivery vehicles for gene editing and therapy applications, offering a safer and more versatile alternative to viral vectors, which face challenges like immunogenicity, limited capacity, and high production costs 1,2 . Polymers bind and compact DNA or RNA cargo into nanoparticle complexes, called polyplexes, for delivery into cells . Among the most popular polymer systems is polyethyleneimine (PEI), sold commercially as jetPEI ® , which is widely used as a basic scaffold for delivery systems employed in routine transfection and gene editing applications .