However studies in cell-sized unsupported model membranes are missing that would provide greater fundamental insight into the dynamic processes that might occur in unsupported membranes seeded with topologically-active MO-LCNPs. Here we bridge this gap by applying time-resolved confocal fluorescence microscopy to study the interaction between MO-LCNPs and DOPC-GUVs and the dynamic processes during re-equilibration. We reveal a rich diversity and interplay of membrane topological and shape changes that mimic many of the processes of living cellular membranes. These findings shed new light on the potential cellular interaction mechanisms of LCNPs in drug delivery systems as well as underpin the potential of LCNPs as topologically active triggers for membrane remodelling events in artificial cell technologies.