Nuc-DAOTA + also explored for the real-time dynamic visualization of mitosis in live CHO cells and confocal images are recorded after a regular interval and identified qualitatively by focusing on a single cell differentiation. The Nuc-DAOTA + stained live cells were monitored by capturing images every 10 th minute to analyze the cell division process for a time period of >10 hours. Thus, the high photostability of Nuc-DAOTA + enabled it to perform well under a prolonged period of excitation which was essential for the dynamic monitoring of mitosis in live cells, photostability that is lacking in the most popular nucleus staining probes like Hoechst. From the following set of images, we can qualitatively track the whole mitosis process of a single cell and identified different phases of the cell division (Figure ).