We first used a rapid 1 H- N HMQC experiment to observe hydrogen bonding in [2]-rotaxane 1, which contains two unique bipyridine nitrogen environments, prepared via active template CuAAC chemistry (see ESI, Fig. ). Rotaxanes are mechanically interlocked structures in which an axle is threaded through a macrocycle. Dissociation is blocked by sterically large "stoppering" units. The steric crowding imposed by the mechanically interlocked thread and ring, enforces non-covalent interactions between the two regardless of solvent polarity, making interlocked structures useful platforms for probing throughspace interactions. The elementary approach utilized an HMQC pulse sequence from the Bruker® preset sequence (Fig. ) with 1/2J delay set to 0.0625s (J = 8 Hz) to utilize nJ couplings in the polarization transfer between nuclei. The combined relaxation delay and acquisition time was set at 650 ms to obtain a fast repetition rate without detrimental loss of signal to noise. SOFAST sequences with repetition rates of 0.3-0.4 s did not detect long-range couplings over +10 hours of acquisition in our systems. Measurements were acquired using a standard TBO or a TXI probe operating at 263 K to minimize molecular motion between the axle and macrocycle components. The elementary nature of this approach derives from the optimization of an experiment on many Bruker® spectrometers. This approach can easily be transferred to spectrometers from other manufacturers.