For the resolution of isomers THC and CBD (m/z 315) DMS conditions were optimized regarding LC solvent type, concentration (MeOH, ACN; IPA) and flow rate (8-100 µL/min). It was found that a short gradient (4 min) from 77-95% acetonitrile at 100 µL/min gave the optimum results. Figure , shows the overlaid CV values at SV 600 V for the cannabinoids, under these conditions the isomers THC/CBD overlap at a CV 8.3 V. In contrast, in the LC dimension the isomers are completely resolved at retention times 3.24 min for THC and 2.51 min for CBD as show in Figure .