On the other hand, the spectra of horizontally (east/west) oriented fibers on regions 1 and 5 (Figure , white circles) displayed higher intensity at 480 nm in the blue spectral region, suggesting the most prominent blue-polarized emission from (R)-1NDI fibers. The slightly tilted fibers at sites 2, 5, 6, and 8 (Figure , white circles) showed an intermediate ratio of I540/I480 compared to the east/west oriented fibers. Hence, the different orientations of the fibers with respect to the excitation beam have a direct influence on their polarized emission properties. Practically, the deposited samples of (R)-1NDI + (S)-2PDI mixture on glass substrate show supramolecular nanofibers with a large diversity of orientations () and result in many directions of transition moments of (R)-1NDI and (S)-2PDI chromophores with respect to the sample reference axes, hence a wide variety of polarization values (P). In well-ordered regions, the (S)-2PDI fluorophores are expected to be incorporated on the (R)-1NDI nanofibers and can be excited either directly or by energy transfer from the (R)-1NDI molecules. However, in regions where disorder is dominant, the (S)-2PDI molecules have limited interactions and proximity with the fibers, and energy transfer from (R)-1NDI nanofibers to (S)-2PDI molecules is not favored.