Cytochrome P450 is nature's ultimate enzyme which can catalyse a plethora of chemical reactions. It is biological machinery that works in a well-organized catalytic cycle by using molecular oxygen and water molecules as fuel for the efficient oxidation of several compounds of commercial and medicinal importance. Due to the catalytic versatility of P450 enzymes it is no surprise that Cytochrome P450 is the most widely used scaffold for bioengineering of new catalytic functions. Recently, three members of the CYP450 superfamily; a P450OleT from peroxygenase family , a P450GcoA from CYP255A2 and AgcA from CYP255A1 family were found to catalyse reactions of potential biofuel importance. P450GcoA, in particular, gained special attention as it catalyses the downstream lignin degradation which can potentially be used to produce a drop-in biofuel. Lignin is ubiquitous in the biosphere and it alone constitutes 15-30% of Lignocellulosic biomass (LB). Moreover, the pulp and paper industry alone produces around 50-60 million tons of lignin each year which further adds to this enormous renewable carbon feedstock. Lignin biomass, therefore, is an unexploited treasure that provides an excellent source of sustainable aromatic carbon. Hence, understanding the mechanism of downstream lignin breakdown for potential biofuel production could be pivotal for the bioengineering of this natural enzyme to enhance biofuel production.