Recombinant hBChE was produced in Chinese hamster ovary cells in a form devoid of its Cterminal end and where four residues were engineered to reduce N-glycosylation, as described previously. Protein was purified with an initial BChE-specific affinity chromatography (Hupresin, CHEMFORASE, Rouen, France), followed by polishing and desalting with size exclusion (Superdex 200, GE Healthcare) chromatography. hBChE crystals were obtained using the hanging drop method at 293 K using a 10 mg/mL protein solution and 0.