Despite the described benefits, there are areas for improvement. These include the development of custom-made software solutions to identify corresponding fragments ions, increase sodiated lipid ion intensities for RPLC-MS n , and improvement of E/Z separation for assignment of DB geometries in lipids with multiple DBs. These shortcomings will be addressed in future studies aiming to overcome these challenges. With the presented methodology and potential future improvements, we envision to study the intricate effects of heart diseases on structural features of lipids to link these structural changes to altered metabolic processes after myocardial infarction or heart transplantation.