This study describes the encapsulation synthesis development, integration, and delivery of mRNA with ZIF-8, which is a Metal-Organic Framework (MOF) that is widely utilized in biomedical applications due to its low in vitro and in vivo toxicity. An innovative one-pot coprecipitation method is employed for this purpose. Given the inherent fragility of mRNA, this investigation commences by delving into synthetic strategies and handling techniques that preserve the mRNA's expressibility. Specifically, the stability of the mRNA and its expression in vitro is systematically evaluated, considering the impact of ZIF-8 synthesis conditions, ZIF-8 incubation, and encapsulation within ZIF-8.