The SCX study idenNfied the M5_A2G0F glycan variant ranging from 2.65% to 3.68% for the four DP batches and DS. The next glycan pair observed at consistent levels for the four DP batches and DS was the A2G1F_A2G2F proteoform ranging between and 2.63% to 2.95%. The glycosylaNon data for SEC and SCX analysis is visualised in Figure and Figure respecNvely. During the initial MAM study, peptide clipping was observed for the HGEGTFTSDVSSLEEQAAK peptide.