In the second set of mixed compounds, a total of 9 different saccharides, along with a similar scaffold (L-Ascorbic acid), were weighed in with relative amounts ranging from 3.0 to 27.5% (Mixture B). The components of this mixture were: , Supplementary Information). Separating some of the saccharides using size or affinity chromatography based on hydrophobicity or charge is challenging due to their chemical similarities. For instance, four of the saccharides, L-Arabinose, D-Xylose, D-Galactose, and D-Glucose, are pairwise chemically identical and differ only in the position of a hydroxyl group (diastereomers), which requires stereochemical separation. Trehalose and Maltose are also chemically identical, consisting of two glucose entities and differing only at the glycosidic linkage sites of the two pyranose rings. Similarly, sucrose is only one methylene group larger than maltose, which is similar in size. However, the crystallographic unit cells for all of these compounds are distinct from each other and easily distinguished in MicroED, making crystallographic analysis of chemically identical but stereochemically different compounds possible.