Cholesterol is an important and crucial biomolecule, predominantly localized at animal cell membranes where it serves as an essential regulator of lipid bilayer fluidity, rigidity, and permeability . Cholesterol also plays an important role in modulating lipid lateral organization by promoting the formation of liquid-ordered phases . As such, it has been implicated as one of the main components in the lipid raft hypothesis . The impact of cholesterol extends beyond the lipid membrane, as it can interact with a variety of proteins, acting as a necessary cofactor for the correct functioning of several processes . Metabolically, cholesterol is also a direct precursor of steroid hormones 2 , such as progesterone, testosterone, cortisol, and estrogens. While cholesterol is predominant in animal cells, other related sterol compounds play similar relevant roles in other kingdoms , such as phytosterols in plants, ergosterol in fungi, and hopanoids in bacteria. Additionally, cholesterol and other cholesterol-based molecules have recently piqued biotechnological interest, as they have been shown to be crucial components in the liposome-nucleic acid complexes deployed as delivery systems in gene therapy and mRNA-based vaccines . Molecular dynamics (MD) simulations provide molecular-level structural detail into the dynamics of cellular processes and systems which may not be fully accessible with experimental techniques. MD has allowed researchers to probe specific interactions between cholesterol and other membrane partners such as lipids or proteins; particular biophysical properties such as flip-flop, interdigitation or membrane permeability; study phase-separation and the formation of lipid domains; cholesterolmediated protein sorting; and even the biophysical properties of curved lipid membranes. Overall, MD studies have offered a more detailed view and better understanding of cholesterol biophysics . Coarse-grained (CG) MD simulation methods, such as Martini , can take these studies one step further, allowing for simulations of systems at micrometer and millisecond scales while maintaining near-atomic level detail.