In conclusion, we have synthesised and validated the first photoaffinity pull down probe based on PI5P (9). The probe was furnished using a concise synthesis that involved a key enzyma,c desymmetriza,on step. The ability of the probe to bind and under-go photo-crosslinking to hUHRF1 and other known PI5P binders was confirmed using gel electrophoresis. The covalently cross-linked probe was subject to a 'copper click' CuAAC reac,on with a TAMRA dye, demonstra,ng that the probe can be func,onalized to visualize bound proteins.