We set up lipid bilayers of 200 lipids (100 in each leaflet) in a cubic periodic box of dimensions ≈ 8 nm x 8 nm x 10 nm using insane Parameters/tree/main/tools/resources). For each system, the simulation temperature matches the experimental temperature using the v-rescale thermostat (τT = 1 ps) and we maintain 1 bar pressure using the c-rescale barostat (τp = 4 ps) using semi-isotropic pressure coupling for both equilibration and production simulations 191 . Each system was minimized and equilibrated for 50 ns, followed by a 100 ns production simulation.