A representative example of a phytantriol cubosome is shown in Fig. A. These particles differed morphologically in that lamellar exteriors were less bleb-like compared to monoolein cubosomes, and presented as woven patterns close to the highly ordered particle centre . In general, particles were more defined at their edges, and their overall shape seemed to show a partial size dependency, with smaller particles adopting a spherical shape and larger particles more faceted/crystalline. It was presumed that morphological heterogeneity reflected various stages of particle growth. In contrast to monoolein cubosomes, F-127 does not incorporate into the lattice core, acting only as a steric stabiliser. Occasionally, rod-like morphologies were observed. Fast fourier transform analysis of a section of the particle shown in Fig. A (marked in blue) gave a characteristic cubic pattern to which miller indices were assigned. From iFFT analysis (Fig. ), d-spacing was estimated to be 50 Å, with a lattice parameter (a) ~ 70 Å. Both values were consistent with literature examples of a pluronic/F-127 system. It was noted that phytantriol-based cubosomes could not withstand the same electron beam intensity without loss of resolution compared to monoolein cubosomes, most likely due to their lower lattice parameter. Fig. E Bar chart showing % of Im3m cubosomes and % of vesicles for a total of 40 images (>150 particles).