Then, 9 µm slices were cut and placed on a poly-L-lysine coated microscopy slide. The sections were then washed with PBS and permeabilized with Triton X. After washing, blocking was performed with 1:20 diluted goat serum (ThermoFisher) in PBS for 30 minutes at room temperature (RT). Next, the primary antibody (Abcam, ab47943) was added for 1 h. After washing with PBS, the secondary antibody was applied for 1 h and after further washing steps, slides were dried and mounted with 4,6-diamidino-2-phenylindole (DAPI)-containing fluoroshield medium (Sigma). Overnight incubation was performed at 4°C before imaging on a fluorescence microscope (Keyence BZ-X810).