Targeted multi-analyte methods are commonly used for human biomonitoring (HBM) of xenobiotics, although most approaches assess only a relatively limited number of different exposure markers or chemical classes ). However, recent initiatives aim to expand the coverage of such multi-analyte and multi-class HBM methods to a larger range of xenobiotics,e.g. , who simultaneously assessed more than eighty chemicals with known affinity to the estrogen receptor in relevant biological specimens (blood, urine and breast milk).