None of our synthesised block copolymers self-assembled into vesicular morphologies, which would have been observed as membranous structures in the cryo-TEM micrographs and distinct SANS curves that could be fitted with a Hollow sphere model as shown previously. This is in disagreement with previous studies on similar PEG-b-PAA-b-PNIPAM block copolymer assemblies, which highlights the challenge of using theoretical rules to predict the final structure and encourages the use of various complementary techniques to precisely evaluate morphology. A combination of techniques such as DLS, cryo-TEM and scattering techniques like SANS, small-angle X-ray scattering (SAXS) and combining dynamic and static light scattering (DLS/SLS), is necessary to obtain a clear picture of the size and morphology of self-assembled nanostructures, which allows identification of the type of nanostructure formed.