In addition, the PM 2 demonstrated reasonable performance in matching microalgae biomass growth (Figure ) and the accumulation of carbohydrates (Figure ) and lipids (Figure ) triggered by the nutrient depletion, with RMSEVSS of 27.82 g-VSS•m -3 , RMSECH of 6.02 g-COD•m -3 , and RMSELI of 4.82 g-COD•m -3 , respectively. In this experiment, as orthophosphate was depleted, we observed increases in carbohydrate content (from 13% to 23%), lipid content (from 12% to 14%), and the carbon:nitrogen ratio of the biomass (from 5.33 to 5.93). Thus, it was demonstrated that the PM 2 can reasonably match experimental observations of biomass growth in lit conditions (457-990 µmol•m -2 •s -1 ) and biopolymer storage under phosphorus limitation.