For the synthesis of crude protease enzyme, we followed a systematic procedure, presented in Figure . The washed mortar and pestle were used to grind a 5g piece of chicken intestine into a thick paste. This paste was mixed with distilled water, sonicated at 4°C for 10 minutes, and then stirred using a magnetic stirrer for an additional 10 minutes. The resulting mixture was placed in Teflon tubes, centrifuged, and left to settle. The supernatant collected from this process was designated as Crude Protease Enzyme Extract (CPE).