The algebraic formalism discussed in this report assumes throughout that in the absence of inhibitors the time course of the enzyme assay is strictly linear, in the sense that the reaction rate effectively does not change over time. In the simplified case of zero inhibitor depletion, i.e., in the absence of tightbinding, Cha has shown that the time course of an inhibition assay is described by Eqn , where F is some observable experimental signal such as fluorescence; F 0 is the baseline instrument offset; v 0 is the initial reaction rate; v s is the reaction rate at steady state; t is the reaction time; and k obs is the apparent first-order rate constants. Note that unlike the closely related Eqn. (34) in Cha's original report , the reaction rates v 0 and v s appearing in Eqn (1) are expressed in instrument units (e.g., fluorescence units per second) as opposed to in concentration units (e.g., micromoles of product formed per minute).