This was mainly due to the challenge of producing false-color images of several cells by using manually supervised map analysis (i.e., univariate or cluster analysis at single cell level). Similarly, Leszczenko et al. recently reported Raman imaging of ALL cells from patients, but the imaging approach was mainly used to extract 2/3 main clusters used as an input for further chemometric analysis on averaged spectra . In both cases, the analytical strategies only partially exploited the spatial information provided by Raman imaging.