GUVs were electroformed on indium-tin oxide (ITO) conductive electrodes in HEPES buffer (pH 7.4) containing sucrose and a small amount of MOF sample, and resuspended them in a glucose containing buffer following a literature procedure. This facilitates vesicle imaging, as the encapsulated sucrose solution in the GUV is more dense than the glucose solution surrounding the vesicle causing them to settle near the bottom of the imaging wells. Yet, the electroformed GUVs sedimented this way are still mobile.