In traditional cell electroporation experiments, we often use aluminum electrodes, but which can lead to leaking of Al 3+ ions, causing cells damage. Taking this into account, we try to employ tweezers NPDM in detecting the Al 3+ formed during the electric shock process. As shown in Fig. , before the electric shock， as the cells were treated with NPDM, there were faint fluorescence signals in the green channel, while the obvious green fluorescences were observed after the cells were subjected to an electric shock. Intriguingly, the green fluorescence signal in the group of low NPDM concentration is stronger than that in the group of high NPDM concentration (Fig. ), which can be attributed to the bistructure of tweezers NPDM. Therefore, it is better to use conductive polymer electrodes than that of the aluminum electrodes to maintain cellular homeostasis and promote cell survival. Noteworthily, it is the first reported smart tweezers capable of verifying the leaking behavior of Al 3+ ions during electroporation, which is beneficial to select suitable electroporation electrode materials in future electroporation researches.