With the above information, chemical reactions and their fractional conversions ( The amount of quench water is linked with concentrations of acid and CNC. The quenching step is carried out before ultrafiltration, where the CNC concentration is set at 1 wt.% in the pilot-scale process . When CNC concentration is 1 wt.%, the acid concentration is calculated as ~ 10 wt.%, which is not sufficient to advance the hydrolysis reaction . In our design specification for quenching, we set CNC concentration at 1 wt.% by varying the added amount of water. For CNC61, achieving 1wt.% CNC requires a rate of water addition of 80 ton/day. For CNC64, the design specification with 1 wt.% contains not only CNCs but also the sugar layer; therefore, CNC64 requires more water, which is calculated as 114 ton/day.