DNA denaturation plays an essential role in many biological processes. The study of forces maintaining the integrity of the double-stranded DNA helical structure is essential because of its central role in biotechnology and medicine. Analyzing DNA denaturation is vital to understanding fundamental genetic processes such as replication and transcription. Other applications of DNA denaturation are bioanalytical methods and molecular diagnostics. One such method is PCR (polymerase chain reaction), where controlled denaturation/renaturation of DNA is a critical step. DNA denaturation plays a central role in applications such as gel electrophoresis, denaturing gradient gel electrophoresis, and sequencing by denaturation. There are applications where the use of partially or completely denatured DNA is used, such as nanotechnology, the creation of nanodevices, new or improved medical applications such as new drug delivery systems, study drug-DNA interactions, analyzing forensic evidence, designing molecular memories, and building functional DNA sensors including metal sensing, where this process involves the denaturation and subsequent renaturation steps. Thus, we are motivated to study DNA denaturation because of its essential role in improving our knowledge of biological processes, advancing bio-analytical techniques, and improving molecular diagnostic methods, as well as in other medical applications, forensic science, DNA-based sensors, and nanodevice-related applications. Analysis of the literature (see section 3) shows that the intermolecular forces maintaining the integrity of dsDNA have not been sufficiently studied.