For comparison of the composition of surface-adsorbed proteins across the particle chemistry and incubation parameters, SDS-PAGE analysis was performed following the incubation of nanoparticle samples for pre-defined times (2 and 24 hours) and shear flow conditions (0.85 and 8.5 cm/s). Nanoparticles were recovered from incubation media using the centrifugationresuspension process, and the surface-adsorbed proteins eluted from the nanoparticles prior to loading onto SDS-PAGE gels (Figure ). Multiple protein bands were detected for each sample type, predominantly occurring at 60 which was likely bovine serum albumin . Bands corresponding to lower molecular weight species were also observed at 12 and 25 kDa. In the case of amine-modified latex nanoparticles, a larger number of high-intensity bands were observed, which is in agreement with the observed higher total protein content of surface-adsorbed protein.