An increase in the size of a denaturant molecule can decrease the rate constant for the denaturation process (K 1 for DS → SS) and/or the rate constant (K 2 ) for the opposite, renaturation process (SS → DS). The influence of the size of the denaturant's molecules on K 1 and on K 2 can be different, and the total rate of denaturation K = (K 1 /K 2 ) can increase or decrease with the denaturant's size. From this, we can conclude that the selected geometric mean approximation lnK = f (V r ) has two opposite scenarios dlnK dVr < 0 or dlnK dVr > 0. The first one can be described as lnK = (Z/V r ). Here, Z is the coefficient explained in expression 30. The second scenario has a form: