S-892216 anhydrous crystal capsules or solid dispersion tablets were orally administered to dogs (n=3) at 3 mg/kg under the non-fasted condition. After administration, 25 mL of water for injection was orally administered. Blood samples were serially collected from the forelimb vein up to 48 h after dosing and centrifuged to obtain plasma samples. The plasma concentration of S-892216 was determined using LC/MS/MS (SCIEX Triple Quad 6500 and SCIEX Triple Quad 6500+). After extraction with acetonitrile, samples were injected into a YMC-Triart C18 column (3 µm, 2.1 × 50 mm) and eluted from the column using a gradient program (representative), with mobile phase A consisting of 0.1% formic acid in water and mobile phase B consisting of acetonitrile, as summarized in Tables and. The flow rate was 0.75 mL/min. The multiple reaction monitoring precursor/product ion transition was m/z 522/368.