The αSyn fibrils were prepared using a single monomer batch which was transferred to different buffer conditions (200 μM final monomer concentration) followed by incubation in the benchtop thermoshaker with constant agitation (1,200 rpm) at 37 °C for 7 days. Fibril samples were frozen and stored at -20 °C. Prior to the experiment, fibrils were thawed and sonicated using probe ultrasonic (Heilscher UP200St). Sonication was carried out in repeating intervals of 3-second pulse with 20 % amplitude and 12-second pause for 5 minutes (one minute total sonication time) to prevent sample overheating.