The present work represents the first example of a polymeric deep eutectic solvent based on alginate, compatible with stablished spherification processes (crosslinking with calcium) and supporting their application towards the development of biosensors. The formed beads displayed a narrow size distribution (2.9 ± 0.1 mm) and a permeable structure with large internal cavities (25-100 µm). These ALGPODES beads were able to outperform the control beads in terms of their ability to both retain the redox dyes (small molecules) and stabilize the enzymatic reporters, aspect that was attributed to the presence of the NADES phase stabilized by hydrogen bonding. The applicability of these beads towards analytical applications (lab-on-a-bead) was demonstrated by integrating three sensing platforms (GlutOx/HRP-TMB, GOx/HRP-ODI, and LOx/HRP-AAP/DHBS) and by their application in real food samples. We believe the simplicity of this approach, coupled with the convenience of smartphone-base sensing, are competitive advantages with respect to other point-of-care platforms.