The plasma membrane bilayer's lipid composition is well-established as asymmetrically distributed ). This asymmetry is critical in maintaining cellular health, regulating membrane-dependent processes, and tuning integral membrane protein activity . Specifically, lipids such as phosphatidylcholine (PC) and sphingomyelin (SM) are predominantly localized to the outer leaflet, while phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS) are concentrated in the inner leaflet . Disruption of this transbilayer asymmetry, particularly through the exposure of cytosolic lipids like PS to the exoplasmic leaflet, profoundly affects cell fate. For instance, the loss of PS asymmetry has been linked to various blood disorders, thrombosis, and metabolic syndromes . To preserve this essential asymmetry, cells invest significant energy through ATP-dependent flippase and floppase enzymes that actively maintain the lipid distribution within the plasma membrane.