Bare SPE, GOx-coated SPE and SANs/GOx-coated SPE were analyzed in a 2.5 mM glucose solution using CV with the CHI660E electrochemical workstation. The SANs-coated SPE was measured in 0.01 M PBS using the same method. The amperometry method was applied for glucose detection at -0.22 V via CHI660E electrochemical workstation. D-glucose was dissolved in 0.01 M PBS (pH 7.4) to prepare glucose solutions with a concentration range of 0.1 μM to 40 mM for the calibration curve. 70 uL of glucose solutions were dropped onto the three electrodes of SANs/GOx-coated SPE and GOx-coated SPE respectively to obtain the response currents to varying glucose concentrations at -0.22 V. Then, 5 mM uric acid, L-alanine, lactate, and ascorbic acid were prepared for interference test. 70 uL of interference solutions were added onto the electrodes of SANs/GOx-coated SPE to analyze the response current using amperometry at -0.22 V. Seven identical SAN/GOx coated SPEs were made using the same method and then tested with 2 mM glucose solution using amperometry. Meanwhile, the SAN/GOx coated SPEs were stored at 4 ℃ and room temperature for one month and tested with 1 mM glucose solution using amperometry every week.