PBS/M-PL, PBS/M-L and PBS/M, was not observed after 8 hours incubation, however, a slight OD fall was observed for PBS/M-L after 20 h cultivation. These results confirmed that PBS/M, PBS/M-L, and PBS/M-PL composites were found to be very effective against Φ6 phage. However, considering that PBS/M-PL was slightly more active than PBS/M-L film and that PBS/M-P was inactive towards bacteriophage, it may be concluded that synergistic effects between limonene and pinene were noted. Similar results were demonstrated in the previous study , which confirmed that polyethylene (PE) films containing the mixture of scCO2 extracts of rosemary, raspberry, and pomegranate fruit in the PE matrix exhibited high activity against Φ6 phage. However, the other work determined that biopolymer resin based on PHB/PLA blend modified with Hypericum L., Urtica L., and Chelidonium L. extracts mixture and zinc oxide nanoparticles hybridized with the extracts' mixture showed moderate activity against Φ6 phage. As emphasized in the graph (Fig. ), the PBS/M composite impacted the bacteriophage effectiveness. It can suggest that the antiviral activity might be related to the increasing adhesion of phage on the surface with adhesive properties that could be added into a polymer matrix instead of active/antiviral substances. It corresponds to SEM analysis, which revealed a slightly more developed surface of PBS/M films than the other series (Fig. ).