for each TRIM protein domain (Table ). Three peptides were used in this assay, H31-27K18Ac that detects binding to the BRD, H31-27K9Me3 that detects binding to the PHD, and H31- This compound showed no affinity for the TRIM24 or TRIM33 PHDs when using the H31-27K9Me3 peptide, consistent with the hypothesis that this compound binds to the BRD. Given its similarity to previously reported TRIM24 BRD ligands, we propose that compound 8 occupies the KAc binding pocket of the TRIM33 BRD. Preliminary docking studies, using either MOE or AD4, reveal at least two plausible binding poses for compound 8 (Figure A and B).