We performed extensive molecular dynamics simulations of the full troponin core (419 amino acids) in the presence of Ca 2+ in the bis-phosphorylated (SEP) and unphosphorylated (uP) state for both wild-type (WT) troponin and the cTnC G159D mutant (G159D). We show results for the doubly-negative charged phosphoserine since this is the species likely to be present at physiological pH. Five 1.5 µs runs were performed for each condition and structural and dynamic properties were analysed.