Triplicate fed-batch bioreactor cultures were performed over a 14-day period in 2 L singleuse Univessel'(R) (Sartorius). Culture medium for 2 L bioreactor fed-batch cultures was JM-05B (FUJIFILM Irvine Scientific), supplemented with 8mM L-glutamine. The bioreactors were sampled immediately after inoculation and daily for viable cell count (VCC), viability, extracellular metabolite concentrations and samples for proteomic analysis. Bioreactor sampling preceded daily bioreactor feeding, with cultures fed daily from day 2 onwards using a proprietary feeding regime.