For B100 fuel, the oil extraction phase is the largest contributor in AP, GWP, and HTP impact categories, while combustion phase is the largest contributor in EP impact category. The cultivation phase shows low contribution in overall life cycle which is contradictory to the results obtained in previous studies . This is because most of the earlier studies has evaluated the initial 1-5 years but in this study life span of 20 years is considered so the impact from the cultivation phase has been normalized. In oil extraction phase, majority of the impact is due to electricity use as Indian electricity grid mix is GHG intensive.