We next sought to establish and optimised the MW growth procedure for later cellular studies. We have previously demonstrated that AC stimulation may improve cell viability in a bipolar electrode system. With the introduction of a frequency component, we hypothesised that this could allow for alternative electrical inputs that are less harmful to cells. Tissue damage is often a result of water electrolysis and noxious by-product from chemical reactions; utilising AC stimulation may reduce this due to less charge building up. AC stimulation has been used previously to grow MWs, although these also required the use of harmful agents that would not be suitable for use with biology. Small single drop BPEs (30-50 µm) were incorporated between two FEs, replicating previous DC studies, using varying potential ranges (1-100V) and frequency ranges (1Hz to 1MHz), and stimulation was applied to attempt to elicit MW growth.