Next, the flow rates were applied to the pumps by the algorithm, as assigned by the input file for the specific experiment and continued for a pre-calculated 'sleep' time such that the volume between micromixer and flow cuvette in DLS is filled with the synthesized nanoaggregate solution. DLS measurements were then performed under stopped-flow conditions to avoid laser alignment issues arising from flow. A true transient screening might be faster than stop flow conditions, yet the volume of commercially available cuvettes is too large to facilitate this.