LLC particles loaded with 5(6) carboxyfluorescein were prepared using the standard MHF set-up as previously described, with both the ethanol (lipid at 14 mM) and aqueous (Pluronic F-127 in HEPES/KCl (10/150 mM) at pH 9.5) streams containing 50 mM CBF. Samples were loaded directly onto a size exclusion column (250 µl loading volume; Sephadex G-50 hydrated by HEPES/KCl buffer) and eluted as 300 µl fractions. DLS was used to determine which fractions contained LLCs (found consistently to be column fraction 6 ie. after 1800 µl eluent buffer had been added to the loaded column), along with a release assay, where Triton X-100 (5 wt%; 2.5 µl) was added to 200 µl of each column fraction. The characteristic fluorescence intensity increase as free CBF is released from the LLCs was compared. Fluorescence was analysed using a 96-well plate in a CARY Eclipse UV-vis/Flu. Spectrometer (Agilent), with λex= 492 nm; λem = 517 nm and PMT value = 600 V. Normalised end fluorescence values were compared to a standard curve for carboxyfluorescein to determine % CBF encapsulation.