Firstly, the device needs to offer high conductivity to achieve maximum sensitivity. This required the optimization of the printing layers. As shown in Fig. , the more layers of printing, the dense the film was. An obvious decrease was seen with increasing layers of printing. Arguably, the decrease in the channel resistance began to brake at 3 layers and curbed at 5 layers. As a result, 5 layers of printing was the optimal to achieve adequate conductance, which also endures decent amount of rGO surfaces for Anti-L conjugation that conditioned the high sensitivity, as described in the section for analytical performances. The next optimization to make is the optimal density of Anti-L on the surface of the sensor. As shown in Fig. , during the immobilization step, as the concentration of Resistance (kΩ)