Among reported methods for analysis of triterpenoid content in C. asiatica, the use of HPLC with the addition of β-cyclodextrin, which will interact with the polycyclic carbon skeleton, in the mobile phase, appears to be most effective one, especially for the separation of the two isomeric triterpenes, madecassic acid and terminolic acid or the madecassoside/terminoloside isomers. The resolution of these isomers increases in line with β-cyclodextrin concentration . Other methods include use of a macroporous HPD 100 resin for the adsorption and desorption of madecassoside, and asiaticoside, and their separation from C. asiatica extract . The two isomers and madecassoside can be separated with high resolution on an Atlantis T3 C18 column with water-acetonitrile-methyl tert-butyl ether (80:18:2 containing 0.1% acetic acid v/v) as mobile phase. This HPLC method is specific for the simultaneous analysis of asiaticoside, terminoloside and madecassoside in C. asiatica products with a good sensitivity, precision and repeatability ). An HPLC method for the qualitative and quantitative determination of six triterpenoids: asiaticoside, madecassoside, asiatic acid, madecassic acid, terminolic acid and terminoloside in C. asiatica raw material or in its products was reported using a Phenomenex Aqua 5 μm C 18 (200Å ) column and water (0.1% TFA) : acetonitrile (0.1% TFA) :