The robotic plate handler underwent validation of its core grab-and-release functionalities via automated protocols within the Opentrons platform (opentrons app and OT-2 Jupyter notebook). The plate handler's design accommodates a diverse suite of standard and custom labware, including well plates, reservoirs, tip racks, and a custom LCMS vial holder, due to their lateral surface and dimensions. Geometric limitations currently preclude the use of tube racks. A comprehensive evaluation of the plate handler was performed, testing its ability to transfer labware between deck positions 1 and 9 under varied loading conditions (detailed labware weight data available in the repository). This included both empty labware and labware filled with weights ranging from 42 to 286 g see repository for full details (Figure ). The plate handler 's vertical (z-axis) travel, from deck to bottom of labware reached either 142 mm or 133 mm, depending on labware, both exceeding the 126 mm height of a standard falcon tube in a tube rack, thereby ensuring ample vertical clearance and compatibility. To simulate a realistic laboratory workflow, the plate handler was used to reposition labware across the deck to designated locations amidst other labware, replicating a working laboratory environment (Figure ). Successful completion of the protocol was confirmed via visual inspection.