Telsa Solarix 2XR mass spectrometer (Bruker Daltonics) equipped with a nanomate infusion robot (Advion Biosciences). The resulting mass spectra were then processed using the SNAP algorithm in Data Analysis (Bruker Daltonics) to produce monoisotopic mass lists. The mass lists were then searched against the primary amino acid sequence of PfCLK3 kinase domain343-699 using MS-Fit in Protein Prospector (University of California, San Francisco) and ProSight Lite v1.4 (Northwestern University). For all analyses, error tolerances of 10 ppm were used. This analysis resulted in identification of 523 peptides, representing 59% sequence coverage.