Cells were observed and CLSM images were taken under a LEICA TCS SP8 STED 3X (Leica Microsystems GmbH). The conditions of acquisition for SOµC-Bdpy1 were set in λex = 488 nm, λem = nm and for SOµC-Bdpy1-GSH were set in λex = 560 nm, λem = nm. For SOµC-Bdpy2, the conditions were also set in (λ ex = 528 nm, λem = nm) and for SOµC-Bdpy2-GSH were set in λ ex = 590 nm, λem = nm. All the images were acquired with a z-step set at 1 µm. Laser intensity was adjusted to obtain all images in the fluorescence linear range and photomultiplier tube (PMT) gain and offset were optimized so the images never showed saturated pixels. CLSM images of maximal projections were processed using the ImageJ 31 software.