Figure : Sample workflow, from bioreactor sampling to MRM assay. Samples are taken from bioreactors, and prepared using S-traps. Peptides from control cell pellets are fractionated to produce a spectral library. Global untargeted discovery data are collected on both a ZenoTOF 7600 and a Cyclic IMS QToF. Data are searched using Spectronaut, with a combined spectral library, DIA+ search to increase depth and confidence of identifications. Diagnostic peptides from protein targets are selected for translation into a targeted quantification study on a Xevo TQS. Relative quantities of proteins inform on the status of cells within the bioreactor.