We have developed a new method to reveal forces that maintain the integrity of the dsDNA helix under the assumption that the distribution of the fractional cohesion parameters inside DNA at its melting temperature are identical to that of the fractional cohesion parameters for the components in the solution. This method is important because it allows for the estimation of the degree of influence of repulsion and different attraction forces in DNA during its chemical denaturation. This method can be suitable for selecting DNA (or other systems with controllable denaturation) targeted for new applications as well as selecting denaturants suitable for effective denaturation.