We have previously reported a clear localization of phosphatidylinositols (PI) and (lyso-) phosphatidylinositols (LPI), including the LPI(18:0) and PI (38:4) to the periphery of Aβ deposits . Here, the ratio of the signal localization to the core compared to periphery, is lower in 23mo as compared to 18mo old animals (d,f). This appears to be associated with increased peripheral localization over time, that is not prominent for LPI (18:0) (g,h), but is significant for both PI Interestingly, similarly to previously reported species, the here observed PI(18:0/22:6) exhibits similar fatty acid configuration the secondary messenger, phosphoinositolbiphosphate (PIP2) which reduction has been at the expense of increase in PIs have been observed in the context Aβ oligomerization . Whether the preferential localization of some lipid species might be associated with age dependent plaque maturation, with a gradual increase in localization of lipid species, such as PI(18:0/22:6) to specific plaque sub-regions, or whether this is an analytical artifact is unclear. In this context, we have previously shown in in the context of Aβ plaques in tgAPPSwe mice, that rather than age dependent, there are morphology dependent differences in Aβ peptide ratio (Aβ1-40/ Aβ1-42) . Aβ plaques that were diffuse in nature (respectively cored plaques) did display similar Aβ peptide ratio regardless of the age of the animal. It is therefore likely that morphological difference of Aβ plaques rather than age difference among animals might underlie any differences observed among plaques.