The global analysis gives well defined confidence intervals of the fitted parameters, albeit sometimes at the expense of quality of the individual fits. Finally, we verified the results using deconvolution of the curves by numerical analysis in COMSOL. The depolymerization curves obtained by the four methods are almost identical and yield similar energy parameters (within error) when analyzed within the isodesmic depolymerization model framework (SI figure ). We chose the global fitting approach for analysis of further experiments owing to its reliability, speed, and automation.