Protein G simulations were prepared and run in a manner as similar as possible to a previously published work . A crystal structure for Protein G was obtained from the Protein Data Bank indexed as structure 1PGB. Using Gromacs software, the protein was solvated with a cubic box of TIP4PEW waters with a margin of at least 1 nm in the X, Y, and Z directions. Sodium ions were added to neutralize the system charge. The periodic system was modeled using Particle Mesh Ewald with a nonbonded cutoff of 1 nm, and all bonds were constrained to their equilibrium lengths for simulation. Using OpenMM, the system was minimized, and then simulated at a constant temperature of 300 K, and a constant pressure of 1 ATM with a Langevin integrator with a damping constant of 0.1/ps and a timestep of 2 fs. Equilibration was performed for 5 ns, followed by production simulations of 195 ns. During the production phase, trajectory frames were saved at a frequency to generate 19500 frames. Following the local alignment procedure described above, with a cutoff of 0.75 nm defining all adjacent residues, The MI of all pairs of alpha carbons was then performed using Netsci with a k-nearest-neighbors k value of 6.