Κwith Cr 3+ ion adsorbed were undergone antibacterial activity through "Agar Well diffusion Method". According to this method, the components of the dehydrated medium were weighed and dissolved in one liter of distilled water and heated up to boiling to dissolve the components completely. The medium was autoclaved at 121 °C keeping the pressure of 15 Ibs/in 2 for 15 minutes. After sterilization, the medium was then transferred to petri dishes and left for a few minutes so the components in the media should be solidified. It was then moved to incubator whose temperature was maintained at 37 °C and incubated for a day in order to look for any contamination occurred during the process. Those plates were selected which had no contaminations in it. This solution was prepared by concentrating the samples up to 1 mg/ml in di-methyl sulphoxide (DMSO) and other dilutions made from DMSO were 50 and 100 µg/ml respectively. Media used for this purpose was Mueller-Hinton is summarized in Table . The compounds were used in two concentrations i.e. at 50 µg/100 µl and 100 µg/100 µl. One loop full of 24 hours of old bacterial culture containing approximately 104-106 CFU, was spread on the surface of agar plates. Autoclaved metallic borer was used to construct wells in the medium. The marked area was filled with diluted solutions of the test samples, metal salts, and solvent di-methyl sulphoxide. These plates were incubated at 37 °C for 24 hours. After this period, a clear zone of inhibition was seen which indicates anti-bacterial activity which was then used to measure in-vitro activity shown by the test compound. In our study, chromium sulfate and κ alone did not show any noticeable antibacterial activity. Figure and 3 summarize all the results of the activity.