Pressure sensitive double-sided adhesives such as acrylic and silicone-based tapes have frequently been used for microfluidic device bonding . The adhesive tapes can simply be cut via a laser cutter or a vinyl cutting machine to form the geometry of the microchannels within the tape. Afterwards, the tapes are sandwiched between two flat thermoplastic layers under mild pressures to create an enclosed microfluidic device. Adhesive tapes can greatly expedite manufacturing process of multilayered microfluidic devices and do not deteriorate the transparency of the device 60 . Moreover, as adhesive taps are gas permeable, the intermediate adhesion layer can compensate the impermeable nature of thermoplastic layers, which is a critical factor for cell studies 121 . Another advantage of adhesive tapes is that the substrate can be functionalized and coated with the biomolecule of interest prior to bonding. It is important to consider the cytotoxicity and adsorption properties of the adhesive tapes when they are applied for cell culture and biosensing applications . Sathish et al. used this strategy to bond a major outer membrane protein (MOMP)-functionalized PMMA substrate to another clear PMMA substrate 124 . In their design, the geometry of the microchannel was cut out in a double-sided tape via a CO2 laser cutter and subsequently the tape was sandwiched between the two flat PMMA substrates. Chai et al. incorporated a hydraulic thermal press to enhance the bonding strength of a POM microfluidic device bonded via a pressure sensitive adhesive layer . Mylar tapes have also been laser cut to form the channels and then used for bonding layers made of PS . Bonding properties of dry adhesive tapes for thermoplastic microfluidics are fully investigated in a work done by Tsao et al. .