The nh-PHIP is an NMR technique that detects pH2-derived hydrides of short-lived iridium complexes (Scheme 1). Each analyte forms a distinct transient complex with iridium which hydrides resonate at specific frequencies around -22 ppm, a normally signal-free region of the ¹H spectrum (Figure ). Unlike other pH2 hyperpolarization methods, no polarization transfer to the analytes is required and the experiments are not limited to single-scan. Instead, nh-PHIP allows scan repetition modes similar to conventional NMR spectroscopy. The method has been shown to provide signal enhancements of 3 orders of magnitude above conventional NMR. Our previous work has been focused on achieving nh-PHIP compatibility with urine 6,7 (Figure ), where we have successfully detected several small molecule metabolites related to smoking. However, as urine is a waste product of the body, many of the diagnostically valuable higher energy metabolites such as ATP, ADP and AMP are not renally excreted. In order to detect these analytes in blood sample pretreatment methods need to be developed.