Glucose oxidase remains the most robust, highly specific, and easily produced biorecognition element for glucose detection . Its combination with the AOx scavenger effectively overcomes its primary limitation-its reaction with oxygen-thus making glucose oxidase an ideal sensing enzyme for electrochemical glucose biosensors. Additionally, we demonstrate that the AOx-based scavenger, using either paraformaldehyde or ethanol as the reducing agent, is compatible with other oxidase-based biosensors, such as those for lactate and creatinine. These results demonstrate that the AOx-based biosensor can enable a broad range of oxidase-based biosensors that were previously impractical due to excessive oxygen sensitivity, especially those targeting low analyte concentrations.