The hotspot analysis showed that, except for process step VI, each process step exhibits large impact contributions in at least one category. However, from the heatmap in Figure it is evident that steps I and III incur the overall greatest impact contributions to paracetamol production at the current state of process development, followed by process step IV. This suggests that further process optimisation should focus on process steps I, III and IV, as these hold the highest potential for significant impact reductions upon process improvement.