To compare the e ect of Open Force Field NAGL charges to OpenEye AM1-BCC ELF10 charges on calculated G, we calculate AHFEs in 5-peats for our 6 molecules chosen in Section 3.4 using Openforce field NAGL and compare these to the calculated AHFEs with OpenEye AM1-BCC ELF10 charges from Section 3.4. Because NAGL charges are conformer independent, these 5-peats have identical starting parameters and thus serve as a sanity check that our AHFE calculations are converging in our 10 ns simulations. If the 5-peat AHFEs run with NAGL charges converge to similar values, we can assume that 10 ns is satisfactory for converging our AHFE simulations.