We validate the applicability of the AOx-based scavenger by constructing the first glucose oxidase-based electrochemical biosensors that is insensitive to oxygen within the glucose concentration range relevant to diabetes management. Previously, oxygen interference could only be mitigated by using alternatives biorecognition elements, such as glucose dehydrogenases and glucose oxidase mutants with reduced reactivity to oxygen , or by specifically designing the immobilization matrix for the glucose oxidase to limit oxygen reduction reaction. However, these methods often entail compromises in selectivity, linear range or complexity of the biosensor. Glucose oxidase remains the most robust, highly specific, and easily to produce biorecognition element for glucose detection. Its combination with the AOx scavenger effectively overcomes its primary limitation-its reaction with oxygen-thus making it an ideal biocatalyst for glucose sensing. Additionally, we demonstrate that the AOx-based scavenger is compatible with other oxidase-based biosensors, such as those for lactate and creatinine. This finding suggests that the AOx-based biosensor could enable a broad range of oxidase-based biosensors that were previously impractical due to excessive oxygen sensitivity, especially those targeting low analyte concentrations.