In this article, we show the development of gold-aptamer-nanoconstruct based biosensor, composed of gold nanoparticles and in silico designed aptamer sequence, which can determine the presence of conserved target nucleic acid sequence amongst all known enteroviruses, through demonstration of colourimetric change that can be detected spectroscopically or through use of lateral flow. Gold nanoparticles were synthesised by sodium citrate reduction. The conserved enteroviral sequence was identified through alignment of archetypal enteroviral RNA sequences, from which a specific and complementary aptamer sequence was engineered. Gold-aptamer-nanoconstructs were characterised using dynamic light scattering (DLS), transmission electron microscopy (TEM) and ultra-violet spectrophotometric analyses. Lateral flow assays were fabricated through deposition of a streptavidin band on a membrane that would capture dissociated gold nanoparticles, functionalized with biotinylated nucleic acid, in the presence of conserved target enteroviral nucleic acid sequence.