The degree of methacrylation, representing the proportion of gelatin functional groups substituted with methacrylate moieties, significantly influences hydrogel properties. It affects crosslinking density, mechanical properties, swelling behavior, degradation kinetics, and biocompatibility. Characterizing this parameter is crucial for tailoring hydrogel properties to specific applications and understanding structure-property relationships. Analysis shows clear differences in methacrylate substitution between low-bloom (gel strength ~175g) and high-bloom (gel strength ~300g) porcine skin gelatins (Figure ). Lowbloom gelatin exhibits a steady increase in substitution across methacrylation categories, whereas high-bloom gelatin shows a lower substitution, comparatively. This difference likely arises from variations in molecular weight distribution and amino acid (AA) composition, particularly the availability of lysine, hydroxylysine, and arginine residues, which methacrylate groups primarily target. Higher bloom-strength gelatins, with denser molecular networks and more crosslinking, may limit accessibility to these reactive AAs, reducing methacrylation efficiency. These findings emphasize that gelatin bloom strength significantly impacts methacrylation potential, which is crucial for designing UV-curable hydrogel systems. Tailoring methacrylation based on specific gelatin properties can enhance hydrogel mechanical performance and biocompatibility for biomedical applications.