PepNde mapping analysis was carried out using a Hypersil Gold 2.1 × 150 mm, 1.9 μm C18 reversedphase chromatography column (Thermo ScienNfic, Sunnyvale, CA, USA). Mobile phase A was 0.1% formic acid in water and B was 0.1% formic acid in acetonitrile. Column temperature and flow rate were maintained at 25 °C and 250 µL/min, respecNvely. The chromatographic gradient commenced at 1% B for the first 5 min before increasing to 10% B from 5 min to 6 min. The gradient was increased from 10% B to 35% B from 6 min to 70 min and then held at 90% B from 72 to 77 min. The gradient was lowered to 1% B from 79 to 81 min, increased to 10% B from 81 min to 83.5 min before increasing again to 45% B at 91.5 min to minimise the risk of any potenNal carryover. A hold at 90% B was repeated from 93 to 99 min before column re-equilibraNon at 1% B from 101 to 115 min. These parameters were maintained constant for both the discovery and monitoring phase of the study.