Overnight grown bacterial culture of MRSA (ATCC BAA-1556) and E. coli (ATCC BAA-2452) were suspended at 10 7 CFU/mL in 10 mM PBS (pH 7.4). Aliquots of 250 µL of bacterial suspension were mixed with 30 µL test peptides (15c and 16c) at final concentrations of MIC and 4×MIC and incubated at 37 °C for 30 min. Controls were run without the peptides.