The context provided by the mass spectrum and the molecular structure helps in understanding that the m/z = 391.2387 peak corresponds to the protonated molecular ion of 4a, affirming its molecular formula and structure. [See Supplementary for Full Response] What substitute reaction should be used in minimum number of steps to improve the conversion from S11 to EBL-3025? Identify the image and context that assisted your conclusion. ScholarGPT: Improving the Conversion: 1. **Direct Amination**: -Instead of using a two-step process, a direct nucleophilic substitution (amination) could be employed if the functional groups are suitable for such a reaction. For example, using a stronger base or a catalyst to directly convert the amide to the desired amine in one step. The Set Nebulizer parameter is set to 0.4 Bar. Adjusting this parameter could potentially improve the quality of the total ion chromatogram results. Increasing or decreasing the nebulizer pressure can affect the ionization efficiency and spray stability, which in turn can impact the quality of the mass spectrometry data.