Laccase was immobilized on arginine-functionalized BNNSs (BNNS-Arg). We assessed the performance of the resulting catalyst, BNNS-Arg@Lac, in degrading ATR across a range of simulated water chemistries and compared it with natural laccase (nlaccase). The comprehensive degradation pathways have also been proposed. This work demonstrated that BNNS-Arg@Lac holds significant potential to be applied in natural and engineered environments for contamination treatment.