Measurements were performed using the method based on Jamnik et al. . Additional MS/MS transitions based on the method of were added to expand the analyte spectrum to a broad range of mycotoxins. Detailed information on the corresponding MRM transitions is provided in the Supporting Information (Table ). A 1290 Infinity II LC system (Agilent) was coupled to a QTrap 6500 + mass spectrometer equipped with an electrospray ionization (ESI) source (Sciex). Fast polarity switching in multiple reaction monitoring (MRM) mode was utilized. An established LC method ) was used for the chromatographic separation of the analytes. Briefly, an Acquity HSS T3 reversed-phase column (1.8 μm, 2.1 mm x 100 mm) (Waters) and a VanGuard pre-column (1.8 µm) (Waters) were used. Eluent A was 0.3 mM of ammonium fluoride in LC-MS grade water and eluent B was 100% acetonitrile. A volume of 5 µL of sample was injected onto the system at a 0.4 mL/min flow rate. The column compartment and autosampler were maintained at constant temperatures of 40°C and 7°C, respectively. Elution of the analytes was achieved with the following gradient: 0-1 min, 5% B; 1-1.8 min, increased to 18% B; 1.8-4.2 min, increased to 35% B; 4.2-13 min, further increased to 48%; 13-15.8 min, constant to 90% B; 15.8-17.6 min, flushed with 98% B and 17.6-20 min re-equilibration with 5% B.