To simplify the occlusion analysis, we calculated the proportion of simulation frames that contain a glycan-dimer atom neighbor pair out of the total simulation frames, and ignored the absolute number of atom neighbor pairs in each frame so as to treat any number of interactions as possible occlusion. Since larger glycans will have more possible neighbors than smaller glycans, this also served to normalize the data to prevent a bias for the larger glycans. The 95% confidence intervals for these binomial proportions were estimated using the independent occlusion samples and the Wilson score method, which produces bounded asymmetric intervals and does not require normal approximations for its use . The proportion of frames with occlusion for each glycoform were only compared within its respective set, because no wild-type control could be included as the wild type is not glycosylated.