CBF was dissolved at 50 mM in a HEPES/KCl (10/150 mM) buffer at pH 9.5. LogD values of CBF at this pH were estimated to be between -4 and -6 (using ChemDraw 18.2), thus ensuring the dye's retention in the aqueous compartments of each LLC. CBF was dissolved in the ethanol stream at the same concentration. LLC particles were then prepared as before, choosing an FRR value of 10 which gave particles of 206 nm, 207 nm, and 264 nm for phytantriol, monoolein, and phytantriol/tocopherol acetate particles respectively, with PDI values below 0.1 for each (DLS results shown in Fig. ).