Imaging was then performed on a Leica Stellaris 5 live-cell imaging microscope in single-photon excitation (λ exc = 405 nm for LysoTracker Blue, λ exc = 561 nm for PhDPA-DiKTa g-Odots). For LysoTracker Blue, emission was captured in the range of 425-485 nm, while for PhDPA-DiKTa g-Odots, the range was 580-700 nm. Previous studies have demonstrated strong fluorescence signals in confocal microscopy at g-Odot concentrations ranging from 7.5 ´ 10 7 particles mL -1 to 1.7 ´ 10 9 particles mL -1 . Therefore, g-Odot concentrations for imaging were selected based on these values.