In order to test the antimicrobial effectiveness of films, after 48 h of incubation, films were removed, and meat samples were soaked in 225 ml peptone water (Acumedia) for 3 min. An aliquot of 1 ml was added to 9 mL of peptone water to obtain the initial dilution (10-1). Subsequently, 1 ml of this dilution was placed into tubes containing 9 ml of peptone water to ensure 10-2 dilution by repeating the procedures for the other dilutions to 10-7 dilution.