In region (1300-500 cm -1 ) , are observed the characteristic patterns of most the samples at about a peak at 1281 cm -1 . The 1200-500 cm -1 range is the so-called fingerprint region, where a strong peak (1281-900 cm -1 ) with shoulders at 1105, 1080, 1055, 1030, 1010 and 980 cm -1 is observed for all samples, corresponding to the C-H bending, C-O and C-C stretching vibrations of sugar . In addition, in this region, all the samples show a peak at between 1170 cm -1 and1155 cm -1 .Bands at 920, 865, 820, 780, and 700 cm -1 are characteristic for all pollen samples and can be assigned to saccharides. Again, P13 samples show a typical peak at 617 cm -1 -720 cm -1 . ATR-FTIR spectra analysis points out that the composition of saccharides, proteins, lipids, and polyphenols is different for dissimilar bee pollens. With this perspective, the spectral region 1800-650 cm -1 can be used to perform multivariate analysis, as already reported .