In eukaryotes, biomolecular condensates--membrane-less compartments that concentrate specific sets of biomolecules--spatiotemporally regulate a broad spectrum of cellular functions . Liquid-liquid phase separation (LLPS) or biomolecular assembly via weak multivalent interaction underlies the formation of these membrane-less structures with dynamic liquid-like properties . Consequently, techniques for the engineering and intracellular construction of protein condensates from sequence-level design are of substantial interest in bottom-up approach research to boost understanding at the molecular level and serve as pivotal tools for synthetic biology .