Therefore, because of the robustness of the biomethanation process, it has progressively gained interest and has currently been reported capable of operating well with highly concentrated CO2 sources of both biogas from anaerobic digestion and syngas from thermochemical gasification . The methanogens can utilize both CO2 and HCO3 -for methanogenesis and furthermore use it for biosynthesis of new biomass. The distribution of CO2 and HCO3 -for the biosynthetic reactions in methanogenesis is controlled via enzymatic activity of carbonic anhydrases expressed by the methanogens, which catalyzes the reversible hydration and dehydration between CO2 and HCO3 -16 . The inherent carbonic anhydrases from the methanogens are therefore utilized in the proposed system for the regeneration of the amines and ensures the stripping of HCO3 -(The absorbed CO2) from aqueous tertiary amines. Although the methanogens can utilize CO2, the biomethanation technology has until now been inapplicable