The gene for TA25 (3FCR-QM-W59L) from Ruegeria sp. TM 1040 was codon optimised for expression in E. coli and synthesized and ligated into the pET28b vector by Twist Bioscience, between NdeI and XhoI restriction sites. Plasmid constructs were transformed into E. coli NEB5α and sequenced (MWG Eurofins) using T7 and T7term primers to verify the insert and then transformed into E. coli BL21 (DE3) for expression.