Next, we performed the similar process in presence of different concentration of trypsin (0-2 M), but at fixed concentration of histone (120 M) where no phoresis effect was observed. Importantly, as observed in Figure , introduction of trypsin results transiently formed condensates and the transiency time can be dictated by the amount of trypsin. Interestingly, upon applying 0.1 M trypsin, we observed a continuous band, alongwith free DNA (Figure -g, Figure , SI). With increasing trypsin concentration, band trailing decreases and accumulation of free DNA increased. The most important fact here that band appearance is more continuous than observed in absence of trypsin by comparing data of Figure and. It implied application of electric field can capture many more states at spatially different location when the system is under non-equilibrium condition. It is important to note that we observe only negatively charged states which actually have low histone composition and arguably, the dimension of those complex will also be smaller to move through gel.