The ion mobility data as a function of fragmentor voltage were extracted using CIUSuite2. The arrival time and signal intensity were extracted for the m/z range of interest using batch files. For our CIU data processing, the intensities in each IMS segment were normalized by peak area for each charge state in a given segment, converted to CCS distributions, and then analyzed by using MCR-ALS to extract the pure components information (pure concentration profiles and pure signal species). MCR-ALS is an iterative self-modelling approach that optimizes C (concentration profiles) and S T (here CCS distributions) under constraints, which has been successfully applied in numerous fields. MCR-ALS has been widely applied for process analysis since it provides pure component spectra and process profiles by using the information coming from the mixture spectra recorded during the evolution of the chemical system. The main advantages of this method are that no prior or little information is needed about the nature and composition of the species involved in the process of interest, the possibility to include complementary and physically meaningful information (such as non-negativity of ion abundances) as a mathematical The MCR-ALS modus operandi can be summarized in the following steps: