All primers and probes, purified 2019-nCoV_N control plasmid, and Hs_RPP30 human control plasmid were purchased from IDT (Coralville, IA, USA). The Research Use Only (RUO) QIAamp Viral Mini Kit for RNA extraction was purchased from Qiagen (Hilden, Germany). The qScript XLT 1-Step RT-qPCR ToughMix was purchased from QuantaBio (Beverly, MA, USA). Molecular biology grade water was purchased from Fisher Scientific (Waltham, MA, USA).