Next, the application of the NanoSafety RDF was explored with a specific focus on one of the MIEs, DNA damage, which is a widely studied adverse effect of ENMs. By utilizing the NanoSafety RDF framework, we aim to get insights into the grouping of ENMs based on their ontological annotations, followed by exploring the effects that can be related to specified physicochemical properties, and finally extend our knowledge of relevant AOPs that involve DNA damage.