In the past, saaPCVA has been applied exclusively for a minimal QM region containing substrates only without covalent bonds crossing the QM-MM border. In this work, we additionally apply it to a QM region containing the ligands and additional catalytic active side residues, as well as to a QM region without ligands only consisting of catalytic amino acids. Furthermore, we will inspect whether one gets reasonable results performing saaPCVA for an enzyme structure without solvation shell, and even directly starting from the available crystal structures without pre-processing to save computational effort and time. These simplifications and variants will be further discussed below.