Recent studies have highlighted the potential of natural products in drug development . Haemolymphs, in particular, have been explored for their anti-parasitic properties . In this study, the trypanocidal activity observed in mice pretreated with the haemolymph of crab and cockroach before infection with T. b. brucei aligns with the findings of , who identified Sarcophaga argyrostoma larval haemolymph as a natural alternative to berenil for treating Trypanosoma evansi in vivo. Despite the observed trypanocidal effects of these arthropod haemolymphs in mice, they did not meet key criteria for an ideal vaccine, such as inducing a 60% weight gain, even though zero mortality was recorded in these groups. According to , an effective vaccine should result in a 60% weight gain in experimental animals. The failure of the haemolymphs to meet this criterion could be attributed to the fact that only a single dose of arthropod haemolymph was administered a few days prior to infection, which may not have been sufficient to generate immunity against the parasite. Furthermore, the route of administration plays a crucial role in the quality and quantity of vaccine-induced immunity . suggested that intraperitoneal immunization yields a superior antitumor immune response and tumor suppression compared to other routes (subcutaneous, intratumoral). For full protection, a vaccine should be administered at intervals prior to infection, as full protection is generally not achieved until two weeks after the final dose.