By analyzing three averaged fractions from the Halfdan sample, we observed separations of compounds from low to high DBE and C number distribution by increasing retention time (Figure ). The number of compounds in each fraction increased from 22 compounds (Fraction 1) to 30 (Fraction 2) and 133 (Fraction 3). The molecular size and degree of unsaturation of detected acids were also increased in the latter fractions, i.e., the early fractions contained carboxylic-BNMA derivatives with small aliphatic, monocyclic, and monoaromatic carboxylic acid structures. In contrast, the later fractions contained acid derivatives with bigger aliphatic, multiple cyclic, and aromatic structures. The AIF LC chromatogram presented an excellent separation of m/z 169/171 peaks produced by corresponding carboxylic-BNMA derivatives.