The first example patient run on the TLC cartridge was compared to the HPLC-derived and was found to have an error of 12% for DOX and 4% error for DOL. The second patient sample had a 5% error of DOX and a 62% error for DOL. This shows that the TLC cartridge design can operate at the same level as the traditional TLC plate analysis and allows for successful TLC analysis and DOX quantification. : Successful TLC analysis using the hermetically sealed TLC cartridge prototype. This cartridge uses the layered design shown schematically in Figure . A. The sample has been loaded onto the TLC plate and the cartridge tipped up to let the mobile phase touch the bottom of the TLC plate. The mobile phase naturally wicks up the plate and as can be seen as the darker region of the plate after 13 minutes showing the mobile phase has made it over 75% of the way up the plate. This demonstrates that there is nothing inherent about the hermetically sealed environment that prevents normal TLC development from occurring. B. A fluorescent image of the developed TLC plate inside the cartridge. The plate is still wet with the mobile phase even though it has been laid down flat and the bulk mobile phase has drained away from the plate. This demonstrates normal development of the TLC plate with well separated lanes and bands. There is a full calibration curve and a patient plasma extract sample. The DOX, DOL, and EPI are clearly visible in all lanes. This demonstrates that the hermetically sealed environment does not interfere with the normal development of the TLC plate, nor does it interfere with the fluorescence imaging of the plate. It was found to have 5% error for DOX and a 62% error for DOL compared the gold standard HPLC-derived concentration value. C. A second example of a successfully developed TLC plate in the hermetically sealed cartridge. Here the DOX in the patient plasma extract was quantified using the analysis software. It was found to have 12% error for DOX and a 4% error for DOL compared the gold standard HPLC-derived concentration value.