Before moving to the second building block container, the 360 ° rotAXYS arm moved to a clean solvent container using the 'Move-To-Container' function to clean the needle. The same aspiration procedure was used for the second syringe. The volume contained in the syringes was then injected into the reaction chip using the Dose-Volume function at a flow rate calculated from the desired residence time. Once the syringes were empty, they were refilled with 2.5 ml of clean solvent using the Dose-Volume function and solvent was injected until the remaining part of the reaction plug had passed the reaction coil with the correct residence time. Once the reaction plug had passed the analysis unit and reached the end of the tubing, the Move-To-Container function was used to move the second rotAXYS arm to an empty container, where it was allowed to remain until the reaction plug was collected. The arm then moved back to the waste container position using the Move-XY function. HPLC-MS was used as an online monitoring tool. A stream splitting device coupled the ambient-pressure reactor system in an isolated fashion to the high-pressure side required by HPLC-MS.