Inhibitor Mini Tablet (EDTA-free, Life Technologies, Thermo Fisher Scientific), and then subjected to ultrasound sonification on ice for 7 min (cycle: 10 s pulse, 20 s pause). After sonification, cell free extracts were generated by collecting the supernatant after centrifugation (45 min, 18000 g, 4 °C) and subsequent filtration through a syringe filter (0.45 µm pore diameter).