The results suggest a change in particle-protein interactions when incubated under shear flow conditions with overall trends in size analysis showing significant changes occurring in particle size across nanoparticle surface chemistries in response to different shear flow rates. This is further supported by the shift in zeta potential from (-25 ± 2) at 0.85 cm/s to (-7.6 ± 0.3) at 8.5 cm/s for amine-modified nanoparticles which indicates a change in the protein corona composition (Figure ). The observed shifts in zeta potential for amine-modified nanoparticles were consistent with changes in measured adsorbed protein content, where the most significant increase in protein concentration was observed between static and 0.85 cm/s flow conditions (8-fold increase), with no significant change in measured protein content observed between 0.85 and 8.5 cm/s flow conditions. In the case of unmodified and carboxylate-modified nanoparticles only small changes were observed between static and 0.85 cm/s flow conditions, with a reduction in protein concentration observed at higher shear flow rate conditions (i.e., 8.5 cm/s).