Our study demonstrates the chemical fuel-driven active transport of small molecules from a sender to a receiver across a centimetersized chloroform or dichloromethane barrier. Notably, the activated molecule acts as the transporter, eliminating the need for external pumps, unlike biological examples such as the Ca 2+ -pump. By adjusting the amount of chemical fuel, we control the yield of transporter molecules through repetitive fuel addition. Varying the interface-toaqueous ratio-achieved by shortening the sender length-allows us to regulate transport efficiency. Moreover, we showed that we can selectively transport molecules, which allowed us to sort a mixture of two different types of molecules. In the future, we aim to apply these design principles to transport molecules across membranes, mimicking biological processes. Our findings of directionally transporting molecules against concentration gradients across barriers have implications for designing new types of cargo transport in medical and material applications.