Using the same configuration detailed above, multiple subsequent sweeps of data were collected on individual crystals of biotin and Zn(II)-methionine at 100K and Zn(II)-histidine and Co(II)porphyrin at 293K, where the same angular wedge of rotation was spanned for each crystal, to assess the response of data reduction statistics from these series to increasing fluence. This was done at incident flux settings of either 0.01 or 0.03 e-/Å 2 /s (spot size 11 or 10).