For preclinical studies the currently preferred method to hyperpolarize fumarate is dDNP, but polarization build-up times in the order of an hour are typical, and the expense and high technical demands of this method strongly limit its widespread application . We recently showed that hyperpolarized fumarate can be formed via PHIP , which is advantageous since it is much easier to implement, and 1-2 orders of magnitude less expensive than dDNP . A molecular precursor is chemically reacted with hydrogen gas enriched in the para nuclear spin isomer, and this is followed by a magnetic field cycle to transfer the para spin order from the hydrogen nuclei to the C nucleus in fumarate. It is necessary to polarize the C nucleus for in vivo imaging because the hyperpolarized C nuclei relax back to thermal equilibrium slower than H nuclei, and have a large chemical shift dispersion which allows chemical selectivity. The drawback to producing hyperpolarized fumarate via PHIP is that, in addition to the desired fumarate product, the reaction solutions contain a plethora of additional chemicals; most notably the ruthenium catalyst, unreacted reagents, and reaction side product molecules.