Group 2. To elucidate changes of cellular metabolites due to the omission of quenching, Group 2 represents freshly dried cells. Cells were dried in a vacuum at room temperature and subjected to SCMS analysis without low temperature storage. Group 3. To determine if storage at low temperature can preserve cell metabolites, cells in Group 3 underwent quenching, drying, and storage (at -80 °C for 48h). This group of cells were prepared.