Figure shows a plot of the total membrane surface area of the liposomes (including the membrane surface areas of the internal liposomes of multi-vesicle liposomes) vs. the outer surface area of liposomes with radii >50 nm. The total membrane surface areas were determined from the cryoTEM images, which include the surface areas of both sides of the phospholipid membrane assuming a membrane thickness of 4.1 ± 0.3 nm. As expected, the unilamellar liposomes exhibit a linear relationship in the plot of total membrane surface area vs. the outer surface area of the liposome, while the multi-vesicle liposomes exhibit variations in this relationship due to a distribution of sizes of the internal liposome(s) (Figure ). The distribution of liposome lamellarity shown in Figure can be used to predict the lamellarity of a liposome characterized with resistive-pulse sensing.