Moreover, a scanning electron microscopy (SEM) image using the backscattered electron detector (BSD) is depicted in Figure shows the shadowing effect surrounding the sensing window, due to the added 20µm of silica. This design was implemented to ensure accessibility to the sensing area while maintaining the integrity of the microneedle region, the silica pool is an important step in the design of a functional microneedle area. The protective layer serves numerous functions, starting with addressing the potential contamination encountered during the penetration of the skin . Additionally, the insertion of the sensing elements into the intradermal layers can result in mechanical abrasion and subsequent detachment of the covalently attached molecular biorecognition layer . By etching the silicon wafer, the pillars are formed, with their tips positioned at the surface level while being shielded by the bulk of the wafer. Furthermore, the incorporation of several microns of silica provides supplementary safeguarding for the pillars during the insertion process. Hence, the elevation of the surface height via To recive the most effective sensing devices, the exact length of the microneedle element should be determined. Previous work of the group, as well as additional research on that topic, showed that 1000µm microneedle is the optimal needle length to reach and rupture intradermal blood capillaries networks, while maintaining minimal discomfort for patients . The monitoring and quantification of numerous protein biomarkers is critical for the early identification of a wide spectrum of diseases. Typically, this entails an invasive and unpleasant operation that involves the extraction of a few milliliters of venous blood for diagnostic purposes. Nonetheless, immense progress has paved the way for a potential decrease in the necessity for this procedure. Rapid antibody- antigen binding followed by fluorometric intensity measurements can be applied with the use of minimal capillary blood samples. This method minimizes invasiveness and discomfort while allowing for quick diagnostic analysis.