This study has provided valuable insights for both collecting additional patient information as well as designing future sample collection procedures that will enable better consideration of relevant lifestyle factors in the next investigations. While improving dust sample collection is a subject under discussion, it is logistically much easier for cohorts to obtain samples from household vacuum cleaners (with all the additional variability that this collection method brings due to different brands and bags) rather than deploy more advanced collection systems, which require training of both nursing staff and participants, and make consistent application more challenging. Alternative collection procedures are currently being tested with this cohort in other studies. While this current effort involved samples primarily from Germany, it also included a limited number of samples from Turkey to ensure sufficient sample numbers. As the cohort develops and more samples become available, it will be easier to form more geographically consistent sample sets (in addition to better age matching between groups), which will also ease the data interpretation. Nevertheless, this work has provided a vital basis upon which to design future experiments. Additional insights are being gained through the participation in relevant collaborative trials run by the NORMAN Network, including a second dust trial, which is in the concluding phases, as well as the recently initiated NORMAN indoor air trial.