MB is excreted via the kidneys, which endues it another function of intraoperative identification of ureters with NIR fluorescence imaging. Alexander et al. have undertaken a prospective feasibility study in 2013 which is the first-in-human experience of using low-dose MB to intraoperately identify the ureters in NIR fluorescence imaging. In all 12 patients, after a simple peripheral infusion both ureters were clearly identified using NIR fluorescence imaging and the MB injection dose they recommend was 0.25mg/kg .