Beyond their biological roles, synthetic conjugates of fatty acids have attracted increasing attention in materials science and drug delivery systems. Fatty acids conjugated with polymers or peptides have been explored for creating hydrogels , liposomes , and targeted drug carriers , enhancing their stability and functionality. Fatty acids, as simple amphiphiles, always contain two types of amphiphiles, the nonionized, neutral form and the ionized form (the negatively charged soap). They can selfassembly to oil droplets, micelles, or vesicles which depends on their deprotonation state . These self-assembly properties make fatty acids excellent model systems for studying fundamental amphiphilic behavior and for applications in encapsulation and drug delivery. Here we propose modifying fatty acids with different amino acids to explore how the simplest form of conjugation affects their properties and self-assembly behaviors. Amino acid conjugation introduces an amide bond and different side chain that might alter the self-assembly behaviors and structures.