Collection Sites for Bacterial Isolation: Bacterial strains were collected from three sites in the SW Florida (permit number: SAL-19-2113-SR; GPS coordinates: 26.591972, -82.068167; 26.681361, -82.089000; and 26.665222, -82.105278) and one site in W. Puerto Rico (GPS coordinates: 17.978961, -67.213472). On the same day of collection, small portions of each egg mass were surface sterilized by submerging in 70% ethanol (aq) for 30 s, rinsed with sterile water (× 3), and then ground up in sterile water by using a sterilized glass rod. Aliquots of this liquid were plated on various solid agar plates for isolation. Isolation media included marine agar, R2A in seawater, chitin in seawater, and RAM in seawater, all containing 50 mg/L of nystatin and cylcoheximide (see Table ). Isolation plates were incubated at room temp (rt) and monitored for bacterial growth. Upon formation of colonies, colonies were subinnoculated on yeast extract malt extract (YEME) and these processes repeated until pure cultures were obtained.