From 4, we see that the cumulative contacts increase rapidly in multiple regions, indicating that multiple residues make frequent contact with the membrane. Crucially, the residues making persistent contact varies across replicas (Figure ). The most consistent interaction is the DHDDS N-terminus, which makes substantial interactions with the membrane. In several cases, the N-terminus of NgBR (residues 330-350 in 4) also makes strong contact. This indicates that, while h-cisPT can associate with the plasma membrane prior to prenyl-chain synthesis, multiple potential bound conformations are possible without other restraints.