Once the impact of UVO treatment on the surface properties was understood, we sought to investigate how the enhancement in CP wettability translated to supporting and guiding interactions of living matter. We therefore seeded rat Schwann cells onto CP films treated with UVO and studied their growth over 48 hours. We utilised live cell phase contrast imaging to determine the number of cells at regular timepoints and assess cell morphology, in particular eccentricity. Statistical analysis of the cell count was carried out by fitting a mixed effects model followed by Dunnett's post-hoc test. Differences in cell eccentricity over time or between groups could not accurately be determined, owing to the tendency of the cells to cluster together (Supplementary Figure ). As in vitro studies routinely employ tissue culture plastic (TCP) for cell culture, owing to its high surface energy and wettability, it was used as a positive control in this work. Phase contrast micrographs displaying morphology and distribution of the cells on the treated films at 48 hours, shown in Figure , and Figure , show how cell count changes on the different materials over the duration of the study. For the pristine and 5 s exposed films a slow decline in the cell population number was observed after seeding. The lowest number of cells per field of view at 48 hours (66 ± 13) was observed in the absence of UV-Ozone treatment on pDPP3T, which incurred minor improvement after 5 s of UVO exposure (204 ± 62). However, we observed a substantial increase in the cell number compared to pristine film with UVO treatment t ≥ 30 seconds, as shown in Figure . Indeed, the number of cells per field was significantly greater than this control at all imaging timepoints from 6 hours onwards, amounting to cell counts of 974 ± 60 (30 s), 1051 ± 167 (60 s) and 1240 ± 104 (180 s) at 48 hours. This suggests that the observed changes in surface roughness and surface energy contributes to the growth in the cell population on the treated polymer surfaces. Interestingly, the cell growth kinetics appear to improve at the same point where changes in the surface energy and UV-Vis characteristics of the pDPP3T films became apparent, following 30 seconds of UVO exposure.