A 5 µL aliquot of sample was placed on freshly glow discharged carbon-coated grid and incubated for 2 min. Following incubation, the grid was then washed 5× with water and wicked dry with filter paper. The samples were then stained using 3 % (w/v) uranyl acetate for 2 min. Excess stain was then removed from the sample grid by wicking with filter paper. The grids were air dried in a dust free environment. TEM imaging was then performed using a JEOL 1400 electron microscope operating at 120 kV, equipped with a AMT XR611 CCD camera.