The inversion profiles for the Cβ spins were designed based on the chemical shifts statistics found in Biological Magnetic Resonance Data Bank (BMRB) , illustrated in Figure . The amino acid residues except for Cys, Ser and Thr residue are divided into three groups: Group A= amino acids at around 40 ppm; Group B = those at around 32 ppm; and Ala whose Cβ has the unique chemical shifts at ~19 ppm, being isolated from the other residues. There are gaps at around 35 ppm between the Group A and B, and at 25 ppm between Group B and Ala, which we chosen as the borderlines for the BE inversion profiles. Accordingly, we have developed following three BE pulses with specific Cβ inversion window: BE-1 that inverts Cβz below 35 ppm to identify Group A, BE-2 that inverts Cβz above 35 ppm to identify Group B and Ala, and BE-3 that inverts Cβz between 25 and 35 ppm to exclude Group B.