We have studied the chiral arginine dependent foldingunfolding landscape of GAAA tetraloop using multiple collective variables (CV). The CVs (discussed later) were defined in such a way that to understand the conformational flexibility of RNA in folded, misfolded, and unfolded states. We have used a combination of umbrella sampling and parallel bias metadynamics for our further simulations for controlled exploration of phase space related to RNA dynamics. Our results indicate that the misfolded states of RNA have equal free energy stabilities in presence of for both the stereoisomers, while the native folded state get much more stability in the presence of Darginine. Further our single molecule binding studies targeting the native folded state of RNA revealed that the origin of extra folded stability difference in these stereoisomers are originated due to different binding modes and orientational effects caused by these molecules towards RNA motif.