To demonstrate the effectiveness of the current approach, we have tested the DeepWEST method on three systems, namely alanine dipeptide with explicit solvation (Figure ), chignolin with implicit solvation (Figure ), and the NTL9 protein with implicit solvation (Fig- ). Kinetics and thermodynamics obtained from these systems using our DeepWEST approach are compared against the WE and our previously developed hybrid GaMD-WE approach. For the systems mentioned above, we demonstrate that the DeepWEST approach surpasses the WE approach in estimating the rate constants between the metastable states of interest and even outperforms the already established hybrid GaMD-WE approach in many occurrences. We also demonstrate that DeepWEST samples the free energy landscape equivalently compared to the already established approaches. Error bars representing 95% confidence intervals are calculated for all WE, GaMD-WE, and DeepWEST runs. Simula-tions are run until there is no significant change in the average rate constant or "convergence" is reached. Rate constants are plotted with an approximate interval of 50 iterations for each run.