compares the number of unique glycoforms identified for the three MS/MS methods, with UVPD yielding 75 compared to 30 and 31 for HCD and EThcD, respectively. The lower number of glycoforms for all three methods relative to the number identified for TIM1 is due in part to the lower frequency of O-glycosylation of the SEA domain of MUC-16. UVPD identified a higher number of large glycans than that of HCD and EThcD combined (Figure ).