In recent years, multi-analyte fluorescent probes have garnered attention owing to their enhanced precision for the study in question. These systems overcome the problems of using several independent fluorescent probes when they are used for understanding the relationship between more than one biological species. Since, the use of separate individual probes can result in erroneous analytical data due to differential permeability, distribution and reduced biocompatibility. Efforts from our group and others have led to the report of multianalyte systems. For example we have reported on dual-analyte fluorescence scaffolds for the detection of ONOO -and a second analyte. However, in the study of cellular processes, we note that for systems able to detect more than one analyte independently using different emission channels is particularly advantageous and in some cases superior to AND-logic gate systems. Since, dual-channel emission enables the evaluation of each individual species while AND logic systems only provide information on the synergy of the two species.