Extraction solvent (50%, 75%, and 100% ethanol in water, and 50%, 75%, and 100% methanol in water), extraction time (10, 30, 60, 90, and 120 min), and extraction method (shaking, refluxing, and ultrasonication) were evaluated by measuring the amount of bioactive components obtained under each set of conditions. The mobile phase consisted of a gradient elution with solvent A (0.02% formic acid and 0.225% β-cyclodextrin in water) and solvent B (methanol). The proportion of solvent B was 70% for 15 min, then maintained at 100% solvent B for another 10 min. The mobile phase flow rate was 0.5 mL/min.