Using florescence microscopy, we also examined the photobleaching of fluorescently labelled microchips in suspension. The data in Figure illustrate that fluorescence intensity remains almost unvaried for a period of at least 14 days, and only after 21 days a moderate loose of fluorescence intensity is observed. Figure Finally, to gain quantitative surface coverage of SOµC-Bdpy1, a 1 cm 2 silicon oxide piece of wafer (SOµC anchored to the surface) was functionalized as previously described, and the fluorescence intensity of the initial and final Bdpy1 solutions used for conjugation were measured (at emission wavelength of λem = 525 nm). Figures and (Supporting Information) display the fluorescence spectra before and after surface functionalization and calibration curve, respectively, and the data acquired from spectrofluorometric measurements allowed to determine the monolayer density to be ca. 9.7×10 15 molecules/cm 2 , a value consistent with previous studies .