In this study, full-thickness, excisional wounds were made on the dorsum of BALB/c mice with a biopsy punch. The peptide hybrids (at a concentration of 1%) were applied topically. The control group was treated with RADA16-I hydrogel containing no modifications. In addition, four mice were euthanized on day four to examine the early stage of wound healing. The progress of wound healing was evaluated based on photographic documentation collected during the experiment (Fig. ). Accelerated wound healing compared to RADA16-I was observed following application of the RADA-GHK and RADA-KGHK peptide hybrids. In mice treated with these hybrids wound closure began faster and was significantly and consistently accelerated from day 2 up to day 14, compared to the groups treated with RADA16-I or RADA-IM (Fig. ). n day 7 of the experiment, the groups treated with the RADA-GHK and the RADA-KGHK showed more than 40% wound closure, specifically 41.8 ± 16.1% and 44.4 ± 4.9% respectively. In contrast, at that time, the groups treated with RADA16-I and RADA-IM showed minimal improvement to wound closure, specifically 11.1 ± 21%, and 16.2 ± 21.1% respectively. On day 18, complete skin wound closure was achieved for all the tested peptides.