Mice (N = 6) were placed under anesthesia (1.5 L O2/minute, 2.0% isoflurane) in a prone position to facilitate intraplantar administration. To induce inflammation, 50 μL of 100 ng LPS/μL stock solution was injected using a 30-gauge needle into the right posterior paw, while 50 μL of sterile saline was administered to the left posterior paw as a control. Following the injections, mice were monitored in their cages for 1.5 hours with an adequate supply of water and food. At 1.5 hours post-injection, swelling of the inflamed foot was apparent, and the single agent imaging experiments administered intravenously through a tail-vein injection under anesthesia 100 μL of ICG or ICB (100 nmol/mouse, each dye N= 3). Each mouse was imaged using a commercial in vivo imaging station (Ami HT Spectral Imaging). ICB fluorescence images were acquired using 700 nm filter, ex: 640/20 nm, em: 710/20 nm, exposure: 3 s, percent power: 50%, F-stop: 2, FOV:20, binning: medium; ICG fluorescence images were acquired using 800 nm filter, ex: 810/20 nm, em: 870/20 nm, exposure: 3 s, percent power: 50%, F-stop: 2, FOV:20, binning: medium at 0, 1, 2, 3, 4, 5, and 6 hour time points. Dye fluorescence in the living mouse paws was calculated at each time point by analyzing the MPI of a region of interest (ROI) drawn around the right posterior paw or the left posterior paw in equal size. At 6 hours, the mice were anesthetized and sacrificed by cervical dislocation followed by the immediate collection of blood from the heart. The exposed mouse cavity was revealed and imaged. The liver, lungs, heart, spleen, intestine, stomach, pancreas, kidneys, skin, muscles, and both paws were harvested and imaged on a transparent plastic tray. For image processing, the mouse body images were colored "fire" using ImageJ2 software. The images of excised organs were imported to ImageJ2, and a manually drawn region of interest was created around each organ. Mean Pixel Intensity (MPI) of each organ was divided by the MPI of the thigh muscle from the same mouse to give a normalized MPI for each dye.