The resulting annotated assay-target pairs from the first round of annotation were introduced in an annotation feedback loop where they were compared to the original ChEMBL-annotated variants (Supplementary Figure ). Annotations missed by ChEMBL were manually checked to assess their validity and classified accordingly into different categories of true and false positives. True positives included likely correct new annotations and likely correct rescue instances of "UNDEFINED MUTATION" labels in ChEMBL. New annotations and rescues with deletions were also categorized as true positives given the scope of this work. ChEMBL-only annotations were parsed and categorized into different categories of true and false negatives.