Inspection of the multidimensional MSI data showed plaque ROI associated mass peak localizations with overlapping isobaric lipid species as resolved by TIMS. Here, we made use of the additional separation dimension, trapped ion mobility, that allows to visualize isobaric or isomeric compounds (Figure and). Peak separation of isobaric can in theory also be achieved with high resolving power instrumentations such as FTMS. However, acquisition at these mass resolutions require long scan times of about 2-3s per mass spectrum. The TIMS MSI setup allows fully resolve those peaks at much shorter scan times allowing for MSI experiments to be conducted at 10% of the time.