This workflow can be fully automated. Here, we combine information from the BE-CANH and BE-CAcoNH data with an assignment algorithm FLYA . The BE-experiment results were treated as results from amino-acid selective labeling samples. The detailed protocol is given in the supporting information. Figure compares the assignment result obtained in this way (Figure ) with that obtained when the amino acids type assignment is determined only by the Cα chemical shift statistics (Figure ). In the former case, 162 out of 166 chemical shifts were correctly assigned, while without using the BE-code information, only 91 chemical shifts were correctly assigned (Figure ). The four wrong assignments given in the BE-experiments were attributed to the encoding failure of K4, K13 and K28, as these errors were cleared by manually correcting their BE-codes. Notably, in the former case, all correctly assigned chemical shifts were categorized to "consolidated" which means 80 % of the results for the repeated runs are consistent. While in the latter case, only 27 correct chemical shifts were categorized to "consolidated". Thus, the residue type information derived from the BE-experiments is critical for the reliable signal assignments.