Longitudinal measurements of test line intensity over 10 minutes were performed and indicated that maximal cleavage of complexes formed using FabHER-PD-PEG3-SS-PEG4biotin occurred in under 5 minutes (Figure ). More specifically, 82.3% (± 11.8%) test line cleavage was observed within the 1.5 minutes of test strips being interfaced with cleavage reagent, showcasing the rapidity of the cleavage chemistry for triggered release within LFA relevant timeframes.