One of the main objectives of this study was to develop and validate a rapid and selective method to determine oleuropein in dried olive leaves without internal standardisation. The key elements presented here are the production of an oleuropein-free (blank) olive leaf matrix, a validated HPLC method with fluorescence detection (FLD) using spiked placebos (oleuropein-leaves) and cross validation of the HPLC-FLD method with UPCV-UV-MS methods on a different instrument.