Production of 4-VG, 4-VP, 4-EG, and 4-EP was also both strain-and media-dependent . These compounds were only detected after fermentation in red wine and strong golden pale ale, demonstrating the dependence on media, with only the strains that could grow in red wine media producing these phenolic compounds, highlighting both strain-dependence and the connections between growth specificities and flavour production . Surprisingly, even though these B. bruxellensis strains had distinct differences in flavour profiles and growth preferences, sequencing of their DbPAD gene, encoding a phenolic acid decarboxylase, and DbVPR/SOD gene, encoding an enzyme with both vinyl phenol reductase and superoxide dismutase activities, found minimal coding differences between the strains . This highlights the importance of phenotypic analyses, including metabolomics, for strain comparison and selection.