For confocal imaging experiments, CHO cells were seeded in 35 mm culture dishes and incubated for 24 hours. Then the cells were washed with the Dulbecco´s modified eagle medium (DMEM) and incubated with Nuc-DAOTA + (4 μM) in PBS (10 mM, pH 7.4) at 37 o C for 15 minutes. After three consecutive washings with the medium, the cells were ready for confocal imaging by using confocal laser scanning microscope (LSM 980 with airyscan 2, Carl Zeiss, Germany) and Zeiss Zen lite software was used for images collection, processing and overlay analysis. In particular, the real-time dynamic imaging of mitosis in live cells for longer time were acquired first by seeding the CHO cells on a 35 mm dish, stained with Nuc-DAOTA + (4 μM) in PBS (10 mM, pH 7.4) at 37 o C for 15 minutes and then, washings. The cell images were captured after every 10 minutes for more than 10 hours at 37 o C with 5% CO2 exposure and then the best ones were selected for the manuscript.