Including hydrogen bonds in the MFCC approach enters step 2 of the general workflow shown in Figure . Figure illustrates the additional fragmentation needed. Given two hydrogen-bonded amino acids AB, MFCC(HB) results in two capped fragments C and D, and concap E, where the hydrogen bond is preserved, while the standard MFCC approach produces capped fragments A and B, breaking the hydrogen-bond interaction. It is worth noting that MFCC(HB) does not only offer a potential improvement for large biomolecules such as proteins but also for hydrogen-bonding solvents such as water.