The roots of Euclea natalensis were previously chopped, washed, and triturated. The extract was obtained in the Thermodynamics and Supercritical Technology Laboratory of the Federal University of Santa Catarina using Soxhlet extraction, modifying a previously reported procedure . Eight grams of Euclea natalensis powder were placed with 180 mL of ethanol in Soxhlet extraction equipment for 6 hours. The mixture was then rotoevaporated and stored with a small amount of ethanol until it was used to synthesize green nanohydroxyapatite (see Figure ).