The recyclability of BNNS-Arg@Lac has been evaluated through continuous separation and dispersion across multiple reaction cycles (Figure ). The secure peptide binding of laccase to BNNS-Arg minimizes potential leakage during separation, enhancing the reusability of BNNS-Arg@Lac, which was supported by previous research that reported a similar immobilization strategy. This high reusability could also potentially reduce the cost of our catalyst, BNNS-Arg@Lac, further showing the outperformance of this immobilization approach. 40 Enhanced Degradation of Atrazine by BNNS-Arg@Lac. The ATR degradation reaction system was first established under laboratory conditions in a 0.1 M Na-PO4 buffer at pH 3 and 30 °C to achieve better performance of catalysts. Within 24 h, BNNS-Arg@Lac showed ~69% of ATR degradation while nlaccase only showcased ~35% of ATR concentration reduction (Figure ). In abiotic control and inactive laccase control (Figure ), ATR did not exhibit significant change. In the BNNS-Arg group, ATR concentration showed a slight reduction (~12%). This decrease might be ascribed to the partial ATR adsorption on the BNNS-Arg backbone. ATR has an electron lone pair on the N atom, which can be considered as a Lewis base. B on BNNS has a positive charge which can be leveraged as Lewis acid. The Lewis acidbase interaction that occurred between ATR and BNNS-Arg backbone caused adsorption. The adsorbed ATR is likely to have increased access to the active centers of laccases on BNNSs, as the diffusion resistance between the bulk solution and the laccases can be mitigated by the synergistic effect of substrate adsorption. In addition, the enhanced stability of laccase after immobilization may further enhance the ATR catalyzation by retaining the microenvironment of the active sites. Altogether, the overall ATR degradation was raised compared to nlaccase. Correspondingly, two predominant intermediates have been tentatively identified: