ATV-loaded Polymer Micelles ATV-loaded polymer micelles were prepared by thin film method. Methanolic polymer (20 g/L) and ATV (20 g/L) stock solutions were mixed in desired ratio. After complete removal of the solvent at 40 °C under a mild stream of argon, the films were dried in vacuo (≤ 0.2 mbar) for 20 min. Subsequently, H 2 O was added. Complete solubilization was facilitated by shaking the solutions at 1250 rpm at 35 °C for 12 min with a Thermomixer comfort, Eppendorf AG (Hamburg, Germany). Non-solubilized drug (if any) was removed by centrifugation for 5 min at 9.000 rpm with a MIKRO 185 (Hettich, Tuttlingen, Germany). Solubilization experiments were performed with 3 individually prepared samples and results are presented as mean ± standard deviation (SD).