For analyzing the experimental raw data, we first removed the constant fluorescence background using a temporal median filter based on a rewritten ImageJ plugin () . Then, we obtained the positions of the fluorophores with sub-pixel localization precision using a phasor-based localization algorithm implemented in ThunderSTORM available for ImageJ/Fiji . The 2D cross-correlation drift correction was applied (ThunderSTORM settings: 10 bins and 5x magnification). The localizations were visualized using the average shifted histogram option with the magnification set to 5. For the lookup table, we chose magenta and green for the labeled proteins phosvitin and apoB, respectively.