Across all systems studied, the measured dissociation constants range between 18 µM (H26DM-βCD with TDZ and 820 µM (βCD with PMT). On average, the binding free energies are 1.3 standard deviations below the mean (higher affinity) of 1,257 previously compiled CD-guest binding free energies , and TDZ/H26DM-βCD is 2.1 standard deviations below the mean. In fact, TDZ binds each three hosts more tightly than any of the other guest molecules. Overall, methylation at the 2 and 6 positions has only modest effects on affinity, as the maximum changes in Kd from native βCD are only about four-fold across both CD derivatives and all guests. In contrast, as noted above, 3-methylation destroys binding in all cases. Going from native βCD to H6M-βCD produces small, mixed effects on affinity; the greatest drop in Kd, which occurs for TDZ, is only two-fold.