As indicated by the green bars in Figure , residues such as His, Ser, Thr, Lys, and Tyr are observed in proximity to CO2 during its stay in the hydrophilic zone, up to approximately 28 ns. Nevertheless, as CO2 moves from the hydrophilic to the hydrophobic zone, the distribution percentage of potential proton acceptor residues significantly decreases (see the orange bar in Figure ), except for Tyr, which maintains a consistent distribution throughout the simulation.