The explosive development of nuclear magnetic resonance (NMR) during the last several decades has rapidly pinpointed the sensitivity bottleneck of this extremely powerful and versatile technique and accelerated the search for ways to raise the sensitivity. The technological approach of increasing the magnetic field of commercial NMR spectrometers and MRI scanners had soon reached the point of diminishing returns, since moderate gains in sensitivity are offset by increasing cost and technical sophistication. The quest for higher sensitivity was continued along a completely different line, by creating a nonequilibrium polarization of nuclear spins, referred to as hyperpolarization . Progress in this field has led to a plethora of hyperpolarization techniques based on various physical principles and applicable under different conditions . Currently two methods stand out in the context of biomedical research, invivo studies, and advanced MRI: dissolution-DNP (Dynamic Nuclear Polarization) and PHIP (Parahydrogen Induced Polarization) . The excellent efficiency of d-DNP is partly offset by high investment and operating costs and by the slow build-up of the hyperpolarization, hindering wide applications in routine biomedical and preclinical research. The much faster and affordable PHIP method is a better candidate as a broadly used tool. The development of methods for polarization of biomolecules using parahydrogen-induced polarization is therefore of great interest. For the majority of practical applications the preferred option is PHIP transfer from protons to low-gamma nuclei ( 13 C, N, and others) since they are less prone to polarization losses since their relaxation is significantly slower compared to protons, and since one can record background-free NMR and MRI signals.