Moreover, we have developed a microscale physical understanding of the EV-movements within the hydrogel network and their subsequent release from the hydrogel. This is accomplished by initially fitting the experimental EV-release data to a mathematical model of one-dimensional diffusion to determine the empirical self-diffusion coefficient of EVs within the hydrogel. Subsequently, the hydrogel network is probed with model compounds and nanoparticles, and the resulting experimental data are fitted to two distinct established microscale physical models of diffusion. Such physical understanding will enable the future design of hydrogels suitable for various EV-release profiles as required in different medical applications. To our knowledge, this is the first report on sustained EV delivery spanning from weeks to months or even longer. Such an EV-delivery system holds tremendous potential for significantly advancing the treatments involving EVs in general, but especially for repairing corneal injury.