The linearity of the analytical method for quantifying Moxifloxacin was assessed through the preparation of five different concentrations of standards as shown in table . These concentrations included 80%, 90%, 100%, 110%, and 120% of the target Moxifloxacin concentration. To establish the linearity, two replicates of each of the five dilutions, all of equal volume, were injected into the analytical system. The instrument response, measured in terms of average area, was utilized for graphical representation of the concentration versus instrument This comprehensive analysis aimed to provide insights into the accuracy, precision, and reliability of the analytical method for Moxifloxacin quantification across a range of concentrations. The outcomes of these calculations are essential for ensuring the robustness and suitability of the method for subsequent quantitative analyses in pharmaceutical or clinical settings. All range and linearity HPLC chromatograms are included in Figures 6.1 to 6.10 of the SI. The determination of the limit of detection (LOD) and limit of quantification (LOQ) for moxifloxacin through serial dilution (1%, 2%, 3%, 4%, 5%, 10%, 100%) and duplicate injections has been carried out, in a correlation coefficient of 0.999 and a concluded LOQ of 1.00 μg/mL as shown in table . The high correlation coefficient (0.999) indicates a strong linear relationship between the concentration of moxifloxacin and the response signal from the analytical method. This suggests that the method used for analysis is reliable and provides consistent results across the concentration range tested. The LOD and LOQ were found to be at 0.029 μg/mL ,0.095 μg/mL respectively. This indicates that the analytical method is sensitive enough to accurately measure moxifloxacin at relatively low concentrations. All LOD and LOQ HPLC chromatograms are included in