In case of LY3537982, exceptionally low IC 50 values of 95.6 ± 30.0 and 121 ± 5 pM were observed with KRAS(G12C) and (G12C/H95L), respectively. The values against KRAS(G12C/Y96D) were also in the low nanomolar range (IC 50 = 2.8 ± 0.8 nM) (Figure ). IC 50 value is protein concentration dependent, and here limited by the KRAS concentration; thus, the theoretical minimum value for all potent inhibitors in the assay is half of the KRAS concentration i.e., 5 nM, assuming that all the KRAS protein is fully functional. Here, the IC 50 value with GDC-6036 and KRAS(G12C) is close to the theoretical minimum, but the values with LY3537982 are significantly lower than the theoretical minimum.