For agar diffusion assay, sterile filter paper disks (d = 9 mm) were impregnated with 50 μg of the samples using methanol as the carrier solvent. The impregnated disks were then placed on agar plates previously inoculated with Staphylococcus aureus 8325, Bacillus subtilis or Pseudomonas aeruginosa PAO1. The test sample was considered active when the zone of inhibition was greater than 9 mm. The minimal inhibition concentration values were recorded after incubation at 37°C for 12 hours and 24 hours, for bacteria and fungi, respectively. Protocols for agar diffusion and minimal inhibition concentration were followed by The MIC assay using test strains Candida albicans IBT 656 was done by the broth dilution method according to the NCCLS. The test sample was considered active when the MIC values was less than 50 µg/mL. Cell growth and cytotoxicity assay Cell growth and cytotoxicity were assessed using the human epithelial prostate cancer cell line LNCaP (RRID:CVCL_0395) and its hormone-refractory derivative C4-2B (RRID:CVCL_4784), as well as two derivative drug-resistant sublines (C4-2B R and LNCaP R , respectively) (ref is PMID: 33799432), all of which carry wild-type RAS alleles. All cell lines were cultured and maintained in RPMI-1640 medium containing glutaMAX™-I (Gibco, Invitrogen, Carlsbad, CA, United States), supplemented with 10% fetal bovine serum (FBS). Cells were seeded into 6-well plates at 3x10 5 cells/well. After cells attached, the medium in each well was replaced with 2mL of fresh warm medium containing 10µM of the different compounds or vehicle. Cell proliferation dynamics were monitored in real-time using a lens-free Cellwatcher microscopy device (PHIO, Germany). The cell growth curves were generated with the analysis module available from PHIO to determine the total area covered by cells. Cytotoxicity was measured as an endpoint assay of drug-exposure using the CellTox Green Cytotoxicity Assay kit according to