Moreover, based on the scaling relations, the CO adsorption energy can also be considered as an indicator for the adsorption strength of other chemisorbed species. Thus, the possibility of tuning the CO adsorption energy and its comprehensive understanding are highly relevant also for applications. These aspects are addressed in the present work. Building on the previous calculations and experiments we explore the effect of moving an O-vacancy laterally underneath the metal-oxide interface or slightly away from the interface, either laterally or vertically, on the vacancy formation energy (section 3.1), on the charge transfer to the Ru nanorod and to the different Ru adsorption sites as well as on the CO adsorption energy (sections 3.2.1 -3.2.3). Section 3.2 also contains a brief evaluation and discussion of the influence of adsorbed CO on the O-vacancy formation energy (section 3.2.4). In both parts, sections 3.1 and 3.2, we are particularly interested in deriving general principles and correlations such as the empirically established correlation between the position of the center of the d-band on the adsorption site and the adsorption energy on this site, as well as in deviations from these correlations that seem to be specific for supported metal nanostructures as compared to smooth metal surfaces. Focusing on CO adsorption, these are discussed in section 3.2.5. Finally, we will test whether the trends in adsorption energy fit to the trends in the calculated vibrational properties of the adsorbed CO (C-O stretch frequency) (section 3.3).