Independent systems were constructed, each comprised several copies of only one of the following disaccharides: 1GA_0GA, 1GB_0GB, 2MA_0MA, 3GB_0GB, 4MA_0MA, 6GB_0GB, 6MA_0MB and 6MB_0MB (following the naming scheme provided in Figure ). Of these, two disaccharides were chosen for which the bonded term distributions fit well with atomistic data (1GA_0GA, 1GB_0GB), two for which the distributions deviated to an intermediate extent (2MA_0MA, 4MA_0MA) and two for which the distribution deviated to a slightly larger extent (6GB_0GB, 6MB_0MB). The Martini 3 parameters for all of these six disaccharides were generated using the fully automated procedure. A further two disaccharides were chosen for which the parameters were generated using the semi-automated procedure; of these, one disaccharide (3GB_0GB) fit well with atomistic distributions, and the other (6MA_0MB) deviated to a slightly larger extent. Each system was simulated in triplicate at both all-atom and coarse-grain resolutions.