Although the additional fragmentation of P+71 and C+98 linker fragment ions could affect their detection in the MS/MS spectra, their derived fragment ions contain very valuable information on the conjugation site, decrease the number of non-assigned signals in the MS/MS spectra and increase the sequence coverage, an important aspect in the data validation. This phenomenon was more noticeable in HCD (Figure ) than CID (Figure ), because HCD is more efficient CID fragmentation method.