To understand the effect of change in life cycle span, seed yield, and electricity emission intensity on the life cycle environmental impacts from biodiesel over reference fuel diesel, scenario analysis was done. If we evaluate the environmental impacts of biodiesel over the oneyear life cycle span system boundary, GWP impact would reduce by only 2.2% while AP, EP, and HTP impacts would increase by 115%, 215.6%, and 101.8% respectively (Table ). From 20 years life cycle span scenario to 8 years life cycle span scenario, there is a gradual increase in environmental impacts, however beyond decreasing life cycle span from 8 years significant increase in environmental impacts can be seen. This is mainly due to the domination of impacts coming from cultivation phase. So, if a biodiesel plant is in operation for at least 8-12 years then only significant reduction in global warming potential can be achieved, however significant increase in other environmental impact category will still occur. Similar results were reported by Achten et al. that 49% increase in AP, and 430% increase in EP is expected, which confirms the findings that environmental impacts in other impact category will increase from using biodiesel produced from Jatropha.