3D printing techniques have been extensively applied to various fields. Herein, we focus on the recent biomedical applications using 3D printing. 3D printing in the biomedical field can include biofabrication via bioprinting or 3D printing non-biological materials used for medical purposes. Biofabrication is an automated process that generates a product with biological functions from biomaterials, living cells, or other bioactive molecules or constructs using bioprinting or bioassembly, followed by tissue maturation.