To investigate how Man competes with the QR-DiMan-DC-SIGNR binding, S/TEM images were performed on a sample of pre-incubated QR-DiMan•DC-SIGNR complex with 2 mM Man (Figure ). Here, a ~40% reduction of clusters containing > 4 QRs was observed (Figure ), consistent with the significantly reduced mean 𝐷 h observed by DLS (Figure ). In parallel to the decrease in crosslinking, a ~10% increase in the proportion of individual complexes and a ~50% increase in the proportion of nonspecific interactions was observed (Figure ). This result may explain why the 𝐷 h of QR-DiMan did not completely return to its original size after dissociation of protein observed at high Man concentrations.