Transient-category Lys-A-202, (Figure . D). This should the best starting point fragment to grow with LE 0.51, across fragments. This fragment was grown into the compound (PDB-ID; 2W71) and maintained the same interaction with Anchor-category residues except the Glu-A-288. This was a result of substituting the amine group with a methyl and hence lost a critical interaction. The compound gained other hydrophobic interactions with Tyr-A-203, Leu-A-278, and Ile-A-437, and weaker interactions with Non-hotspot-category residues, Ile-A-287 and Gln-A-233. Although, the lead compound is found with better IC50 (0.33μM) than the fragment (IC50 12μM). However, the starting fragment shows a weakened LE over the lead compound, 0.51 and 0.40 respectively. This suggests that keeping the amine group in the vicinity of the Glu-A-288, may have delivered a better ligand efficiency, for the developed inhibitor, (Figure ).