Due to the challenges involved in the chemical synthesis of the entire UL49.5 protein in one synthetic procedure, we decided to initially divide the protein into three parts based on their location in the lipid membrane. Based on literature data and bioinformatic predictions using the PRED-TMR program (PREdict TransMembRane helix fragments TM) we designed three peptide sequences encompassing the UL49.5 protein: the extracellular fragment D1-G35 (N.HSV), the transmembrane fragment A36-I58 (TM.HSV) and the intracellular fragment C59-A68 (C.HSV) (Table ). Out of the synthesized peptides, only N.HSV and C.HSV were soluble while the TM.HSV peptide was insoluble in any of the applied solutions (acetic acid, dimethyl sulfoxide, 6 M urea, water, and methanol).