Here, we reported the preparation of carbon-supported non-noble Ni catalyst starting from biomass lignin raw material. The starting biomass materials and pretreatment of lignin had a great effect on the catalytic physical properties and catalytic reactivity in the selective hydrogenation of alkyne. Under the optimized mild reaction conditions (2 Mpa H2, 80 °C, 5 h), the optimized catalyst Ni/CLW prepared from washed lignin in situ calcination without any addition H2 reducing atmosphere showed excellent conversion of 97% and 87% selectivity in the selective hydrogenation of terminal alkynes. Moreover, the catalyst had good stability and can be reused 5 times without losing significant reactivity and selectivity. It also opens the potential application of biomass carbon materials from lignin directly for heterogeneous catalysis in industry, which is a cost-effective and green environmentally friendly process.