Let us now examine the entire range of pre-IMS activation voltages. Next, we determined the V50 values, i.e., the voltage at which half of the DNA structure is unfolded assuming a two-step conformation, for each condition and charge state using CIUsuite2. A typical result is shown in Figure , and the trends in V50 values are shown in Figure . We see the expected trend that IH and IC are more stable at pH 5.6 than 7.1, and more stable than the CH and CC controls. However,