Similarly to MIBiG, MITE entries will be based exclusively on experimentally verified information, excluding predictions solely based on bioinformatics algorithms. At the same time, MITE will be flexible with regard to the type of experimental evidence and accept data from knockout studies, site-directed mutagenesis, heterologous expression studies, or various in vitro assays on isolated enzymes. Also, inference of the catalytic activity of an enzyme based on a carefully elucidated natural product structure in concert with sequence information would be accepted, e.g. when a NP has one methylation and its BGC encodes a single methyltransferase. All parameters would be annotated using ontologies of evidence attributions that specify the level of confidence of the data, and the corresponding literature references. This will allow researchers to subset the database for entries of a given type of evidence, and exclude entries with evidence that is considered less reliable.