Moreover, For building amber topologies, the recommended charm_radii were used to represent the PBRadii (PBRadii=7). No Entropic estimation was performed. As a result, the calculated binding energy value is not the exact real free energy value because entropy is not considered, but it is still a good representation of the relative binding energies. In the dielectric interface implementation, A level-set-based algebraic method is used. A single term proportional to the solvent's accessible surface area (SASA) is used to model the total non-polar solvation-free energy. Decomposition analysis was performed on residues within 6 angstroms of the ligand.