Samples (150 µL) were taken for processing at 2, 5 and 10 minutes. Blood samples were centrifuged (2,000 × g for 5 min, 4 °C), the plasma was removed and transferred to another protein low-bind Eppendorf. Ice-cold MeOH (500 µL) was added to the plasma and the sample briefly mixed on a Vortex. Samples were then centrifuged (12,000 × g for 5 min, 4 °C), and the supernatant transferred to a glass vial through a Millex 0.