From the click products with other types of substituents (family 5), compounds 34, and 39 were highly toxic against HFF at 2.5 µM. The way in which the substituents are linked to the diruthenium scaffold appears to be important as the triazole-phenyl derivative 34 was significantly more toxic the host cells compared to its analogue 37 (3 vs 17%). The triazole-hydroxymethylene compounds 36 and 38 exhibited similar IC50 values on T. gondii β-gal (0.111 vs 0.128 µM) for medium toxicity against HFF (77 vs 59%).