NMR spectra were obtained on a Varian VNMRS 500 MHz 54mm AR Spectrometer (UoG) operating under VnmrJ software, JEOL 400 MHz NMR ECX-400 Spectrometer (UoG), and Varian VnmrS 400 MHz spectrometer (UCD). Chemical shifts (δ in ppm, J in Hz) were referenced to residual solvent resonances and are reported downfield from SiMe4. Deuterated solvents CDCl3, D2O, DMSO-d6 were obtained commercially and used without further purification. Spectra were visualised and analysed using MestReNova software. Infrared spectra were obtained on a Perkin Elmer Spectrum 400 FTIR/FT-FIR spectrometer equipped with a universal ATR accessory. High resolution mass spectroscopy was performed on an Agilent 6530 Accurate Mass Q-TOF LC/MS coupled to an Agilent 1290 Infinity UPLC system (UoG), Agilent 6546 Quadrupole Time-Of-Flight MS System coupled with an Agilent 1260 Infinity Prime II LC system (UCD) and on a Bruker 'autoflex maX' MALDI TOF/TOF system equipped with a HTX Technologies TM-Sprayer (UoG). TLC experiments were performed using aluminium sheets precoated with silica gel 60 (HF254, E. Merck, Darmstadt, Germany). Chromatography was performed with silica gel 60 mesh (Sigma Aldrich, Wicklow, Ireland). Reactions performed under microwave irradiation were carried out in a CEM Discover-SP Microwave Reactor, 2012 model, in the appropriate bespoke vessels (UoG). All starting materials were obtained from commercial sources and used without further purification, unless otherwise stated. Dialkyne 2, glycopyranose azides, and dpa-based compounds (1Gal OAc , 1Gal and 5) were prepared as previously reported. Expression and purification of recombinant LecA were performed as previously described and the protein was lyophylized. ITC experiments were performed on an MicroCal ITC200 instrument by Malvern Panalytical and all data analysis was performed using MicroCal PEAQ-ITC analysis software (Malvern Instruments). SPR measurements were performed on a Biacore™ X100 instrument using Biacore CM5 gold chips and data analysed with the analysis software (Biacore). All stock solutions were made from lyophilised material prior to use.