To assess the underlying aetiology of enhanced cyclic 1-pass performance and multi-pass underperformance, MsbA data was manually inspected to assess key instrument performance attributes (Figure ). When examining raw mass spectra, ions exhibiting detector saturation were observed in SYNAPT G2-Si data (Figure ). This limits the accessible sample dynamic range and can interfere with algorithmic identification of the relevant peptide(s). Despite having some ion intensities up to an order of magnitude higher, reduced detector saturation was observed in raw data generated on the Cyclic IMS instrument (Figure ); likely owing to its upgraded dual gain analogueto-digital converter (ADC) detector with improved dynamic range and linear response at high ion currents. Thus, decreased detector saturation in cIM data likely contributed to the observed increase in peptide identification by producing data with a wider dynamic range. While dynamic range enhancement (DRE) can be enabled in SYNAPT G2-Si instrumentation to alleviate detector saturation issues , this has the downside of doubling scan times. In contrast, use of the Cyclic IMS appears to allow reduction of detector saturation without reducing chromatographic resolution.