The resulting extracts were dissolved in DMSO and subjected to UHPLC-PDA-CAD-ESI-HRMS 2 metabolite profiling. DMSO effectively dissolves compounds with various polarities, maintains solution stability, prevents evaporation, and it is compatible with biological assays, making it an ideal solvent for NEs dissolution, even at higher concentrations. Additionally, during UHPLC metabolite profiling, DMSO exhibited no specific issues, suggesting its suitability for analytical purposes given the small injection volume (1-2 μL). This enables using the same solution for metabolite profiling and biological screening, enhancing correlation between HRMS 2 data and screening results. The extract solutions in DMSO were kept at -20 ℃ afterwards for preservation.