The primary method to observe GUVs is by using microscopy due to the large size of vesicles (1-100 μm). The protocols for imaging liposomes, however, are limited as the vesicles freely move after harvesting in the solution, hindering the analysis and imaging particularly confocal z-stacking. Typically, a sucrose/glucose density gradient is used for the gravity-based sedimentation of liposomes in the imaging well, while maintaining freedom of lateral movement. Other work in the area extends the approaches by using biotin-streptavidin interaction, DNA immobilization, magnetic, electric, and acoustic field trapping. Strategies involving the covalent anchoring allows for detailed observation of GUVs with an increased risk of membrane modification / deformation and are costly. GUVs have been held in hydrogels and agarose gel to investigate single liposomal membrane properties without any adverse effect on vesicle stability. This limits the use of inverted microscopes, and the hydrogel constituents can incorporate the membrane or lumen of the GUVs which may not be desired for the study. Alternatively, GUVs can be physically trapped in microfluidic channels or chambers/wells for imaging, holding a random population of GUVs. The vesicles compress as they make their way into the channels, hence microfluidic setups can influence membrane elasticity, potentially altering their mechanical properties and making them poor comparisons to physiological cell models. Although these methods offer imaging possibilities, most of them are impractical for implementation.