Myc2345, d(5'-TGAGGGTGGGGAGGGTGGGGAA-3'), was purchased from Eurogentec and used without further purification. Myc2345 was dissolved in a Bis-Tris buffer solution in Nanopure water (10 mM, pH 7.1) to a concentration of 1 mM. KCl was added to the sample at concentrations of 0, 5, 10 and 100 mM. The sample solution was vortexed for 10 s, annealed at 95 o C for 2 m and cooled to room temperature. The sample solution was formed into gel spots by depositing 1 μL on a glass coverslip and allowing it to dry in a sealed sample cell of 85% relative humidity, created by depositing 6 x 2 μL spots of a saturated aqueous solution of KCl. The sample cell comprised of the glass coverslip, a ¼" thick spacer and a 2 mm thick CaF2 disc as the front window. As the spots shrink when they form the gel phase, the final potassium concentration is higher than that of the initial solution.