The retardance of the PII-2T polymer solution was measured as it flowed through the microfluidic device. The retardance was measured using white light in an optical microscope (Nikon Ci-POL) equipped with two opposite handed circular polarizers facing each other and oriented orthogonally to each other with the microfluidic device placed in between them, a configuration also known as a circular polariscope. The retardance is proportional to the birefringence through where is the sample thickness and is the wavelength of light. Birefringence occurs due to the anisotropy of a material's refractive index as it becomes aligned. The measured retardance is an average over the spectral composition of the light source.