A system comprising ds-DNA with 21 base pairs with the placement of the UBP at the middle of the DNA duplex has also been considered to investigate the impact of a larger strand on UBP conformational stability (Scheme 1). Our results suggest the random generation of both WCF and ) predicts a majority of the time the system is in the SYN form during the simulation, whereas occasional distorted structure has been generated with larger dO-S. It is found that RMSD values are comparatively higher than that of short DNA, which indicates that flexibility of the UBP transfers to the entire DNA duplex systems, which is further reflected in high RMSF values of all the nucleotides (Figure ). RMSF value of the DS and DN are also found to be higher for MUDL than other MUD systems which implies that larger DNA duplex is able to give more freedom to the UBP to maintain and amplify its flexible nature. Interestingly like short DNA, here also the RMSF shows similar patterns indicating despite high flexibility, the characteristic of the UBP-incorporated DNA remains similar. High fluctuations of the UBP are also reflected in the high range of DS-DN distance as observed in Figure .