Automated image analysis was performed using Matlab (version 2021a, MathWorks), adapting the method detailed in Baumuratov et al. . The analysis was performed on the HPC platform from the University of Luxembourg in collaboration with the LCSB Bioimaging platform. Features such as mitochondrial mass (sum of mitochondrial pixels/sum of nuclei pixels), mitochondrial size (sum of mitochondrial pixels/mitochondrial counts), mitochondrial membrane potential (mean intensity of TMRE inside the mitochondrial mask), and normalized mitophagy (sum of pixels positive for Mitotracker Green FM and Lysotracker Deep Red/sum of mitochondrial pixels) were extracted.