Table lists the number of mapped reactions that were obtained via direct mapping of the original entry via a single reaction rule application (step 4A in Fig. ), via multiple rule applications (step 4B in Fig. ), as well as via suggesting products based on the reactants of unmapped or unbalanced reactions (step 5 in Fig. ). In total, 63,137 reactions with a unique mapped reaction within an EC class were obtained, not taking into account information on natural/non-natural substrates, organisms and proteins. Disregarding the EC class, 47,974 unique reactions were obtained. When also discerning between natural/non-natural substrates, organisms and proteins, a total of 234,845 non-duplicate entries is obtained. The majority of the reactions in EnzymeMap (71%) stem from single step reactions from the original BRENDA entries. 17% of non-duplicate entries (21% taking into account organisms .