Prediction of the subcellular localization of proteins is crucial for understanding not only the function of the proteins but also the organization of the cell, especially when experimental methods become unable to provide the full coverage of localization. Cytoplasmic matrix proteins are often considered as potential drug targets, whereas inner and outer membrane proteins are regarded as potential vaccine targets. Subcellular localization was confidently predicted for 33 EHPs out of 44, after comparing the results of 3 different tools (Table ). Among them, 66.66% (22) proteins were found to be present in the cytoplasm, whereas 21.21% (7) were for the inner membrane, 6.1% (2) were for extracellular and 6.1% (2) were for the periplasm. Signal peptides play a significant role in the transport of proteins to their target locations and provide information regarding cleavage sites. Four EHPs were predicted to have signal peptides, and eight secretomes were found among all the EHPs. Cell secretomes are proteins secreted outside of the cells that help to regulate cell proliferation, cell-to-cell communications, and pathogenesis . Both HMMTOP and TMHMM servers predicted the presence of transmembrane helices in 11 EHPs for each. These transmembrane helices are important for membrane proteins which play crucial parts in the regulation of energy transduction, signaling, and transmembrane transport in cells as well as for drug development as nearly half of the targets used for developing new drugs are membrane proteins .