Additionally, comparing the WT and G159D, unphosphorylated A/B angles we see that the WT angle is higher at 101.8° compared with G159D at 97.1°. This is further reinforced when considering the percentage of the area under the curve for the A/B angle above 110° which is the angle assigned to be the "open" conformation threshold by Kekenes-Huskey et al. For unphosphorylated WT this is 18% and for G159D it is 1%; the corresponding values for the phosphorylated states are 1%