Figure depicts the size of the coacervates obtained with the four peptides at different concentrations and pH values in the process of coacervation through evaporation (Figure ). The basic assumption in these diagrams is that the more a peptide is prone to coacervate, the more coalescence will occur, resulting in larger coacervates. In this process we can observe the opposite phenomenon, as lower pH seems to promote coacervation and results in larger particles. At lower pH values the peptides obtain higher net charges, increasing their solubility. This could expedite the transport of the free peptides and the smaller coacervates to the droplet's perimeter due to the outward capillary flow. For example, the peptide with the highest net charge is pep2 at pH 4.8 and it also produced the largest coacervates (50 ± 10 µm). Peptides with lower solubility due to their lower net charges could precipitate faster and would not take part in the coacervation. The connection between the peptides' net charge and their ability to coacervate is clear from Figure , which shows that the coacervation capabilities are stronger when the peptides have higher net charges. Additionally, both control peptides AHHA and WAAW do not form coacervates while the peptides Phe-(His)2-Phe (FHHF) and Tyr-(His)2-Tyr (YHHY) do (Figure ). This can be explained by the need of a sticker-andspacer motif that is found only in FHHF and YHHY, while AHHA lacks the hydrophobic stickers and WAAW lacks the hydrophilic spacer.