NMR spectroscopy is a powerful tool being able to analyze protein structures, dynamics and interactions in near-native conditions such as in buffer solutions (liquid-state NMR) , and in lipid bilayers 2 , supramolecular assemblies 3 and phase-separated droplets (solid-state magic-angle spinning NMR or MAS NMR). To access such information site-specifically, one generally cannot avoid the signal assignment process. While it is labor-intensive and time-consuming, once the chemical shift value is determined for each atom, it already reveals a part of the structural and dynamics information, and provides an important basis for all the subsequent analyses.