As we can see the application of Eq 3 is quite limited by coupling strength and energy offset, we strongly suggest high attention to be taken in analyzing experimental results based on it particular at low barrier. In addition, considering that Eq 3 is closely related to several other mathematical models, like transition voltage Spectroscopy (TVS) and law of corresponding states (LCS) , it is very important to be aware of the condition and scope at which TVS and LCS can work effectively.