Active film displays intermediate mechanical effectiveness that is hampered by a high swelling index. The results obtained in the study indicate that the alcoholic extract of mate tea exhibits antimicrobial activity relative to pathogenic bacteria of importance in food (S. aureus). Additionally, the antimicrobial activity is not reduced when the extract is incorporated into the polymeric matrix. Furthermore, combining Ilex paraguariensis extract with biopolymers can correspond to an alternative while developing antimicrobial active food packaging.