Herein, a fully integrated novel array-like device is presented in this work which combines the advantageous architectures of both microneedle-like devices and SiNPs ultra-efficient biomarkers collecting elements as the sensing area. The direct fabrication process of the device results in a fully embedded SiNPs array on the surface of the needles, while each sensing sub-area of the array can be further functionalized with specific antibodies, targeting specific proteins and biomarkers. The needleembeeded nanopillars array allows direct, rapid and minimally invasive capillary whole-blood sampling, while the sensory area of the functionalized embedded SiNPs serves as an excellent bed for immediate analysis of the sample via fluorescence microscopy. The device's diagnostic clinical capability for highly sensitive, selective multiplex detection of protein biomarkers, such as Prostate specific antigen (PSA), which was selected as a preliminary proof-of-concept candidate, was demonstrated in this study. These findings pave the way for near future applications in the detection and diagnosis of additional biomarkers related various diseases of interest.