followed by eluant application onto a Strep-Tactin column (capturing the h-cisPT heterocomplexes). Tags and TRX fusions were removed using TEV protease, and the protein mixture was concentrated and applied onto a Superdex-200 16/60 gel filtration column (Cytiva), pre-equilibrated with 20 mM HEPES (pH 7.5), 150 mM NaCl, and 1 mM TCEP. The purified proteins achieved a purity of over 95%, as determined by SDS-PAGE analysis, and were flash-frozen in liquid nitrogen and stored at -80°C until further use.