The data is recorded for at least 10 min after the addition of the NaCl pulse. After that, a pulse of detergent (Triton X-100, 5% w/w in water, 50 µL) is added to lyse the liposomes. The recording of data is continued until the emission intensity reaches a stable level, which may take 2 to 5 minutes (making a total run last ~12-15 minutes). For experimental consistency and to make the data treatment easier, the NaCl pulse should be added exactly at 30 s, and the detergent at 630 s after the start of the experiment.