For phospholipid binding measurements, mobile phase B was HPLC grade acetonitrile (Chempur, Piekary Śląskie, Poland) with a linear gradient from 0 to 85% B in 5.25 min and then held at 85% ACN for 0.5 min. The flow rate of the mobile phase was 1.5 mL/min, and the IAM.PC.DD2 column was maintained at 30 °C.