The analytical method of moxifloxacin was validated in accordance with the International Conference on Harmonization (ICH) guidelines. A simple, specific, and isocratic reversed phase- high performance liquid chromatography (RP-HPLC) method with UV detection at 293 nm and column Agela Technology, Venosil XBP C18, 10µm, 4.6mm×250mm was developed and validated for analysis of moxifloxacin hydrochloride (MOXI) in presence of its degradation products. The composition of the mobile phase was phosphate buffer and methanol (18:7 v/v) at flow rate of 1.3mL/min, temperature 50°C maintained by using column oven was employed throughout the study. While 0.1N HCl was used as diluent.