Importantly, these two strategies allowed to efficiently immobilize two different TAs (the Sselective HeWT, and the R-selective TsRTA), resulting in stereo-divergent biocatalytic membranes. Additionally, co-immobilization of TA and PLP was also achieved on functionalized PP membranes by adapting the immobilization protocol, which resulted in highly reusable membrane-immobilized biocatalysts capable of catalyzing transaminations in absence of externally added PLP. Such self-sufficient ability should be attractive from an industrial point of view, since it should help increasing the cost-efficiency and reducing the Efactor of the transamination process. Thus, all in all, both studied routes to immobilize TAs on membranes led to functional biocatalytic materials exhibiting perfect enantioselectivity, high catalytic performance, minor leaching and excellent reusability. This paves the way to a future use in flow mode hybrid processes.