The image of the gel for electrophoresis analysis of the PCR product of the CBP purified 1,728-mer is shown in Figure . As can be seen, an expected band can be clearly observed. Colony PCR was first conducted on 16 colonies using primers targeting only a portion of the 1,728-mer (see supporting information for details). All colonies were found to have the gene (Figure ). Plasmids of the 16 colonies were subjected to Sanger sequencing. Sequencing data are provided in the supporting information, and the results were summarized in Table . Among the 16 colonies sequenced, 7 contained the correct sequence, which corresponds to a success rate of 44%. The errors in the incorrect sequences include five substitutions, three single nucleotide deletion, two 2nt deletion and 2 single nucleotide insertion. The sum of the error rates was 0.0434% (Entry 13). Later, we also performed gel electrophoresis analysis on colony PCR products of additional colonies using primers covering the entire 1,728-mer. Among 32 colonies, only one did not show the expected band (Figures ).