In Figure we can see the linear response of peak areas to concentrations. With the characterization of the ESI and detection in our hand, we finally move to our main interest, the integration of areas in the mobilogram. The mixture was prepared at a constant concentration, but run with three different injection volumes (1%, 2% and 3% of the capillary length) to induce peaks of different width. Each was analysed at two pressures (10 and 50 mbar), again in triplicate. This gives a total of six replicates per combination of compound and injection volume.