MV/cm, respectively. The stronger electric field for histone MTases may alternatively be due to the protonation state of the terminal nitrogen. In biological pH ranges, it will be in an NH3 + meaning the methyl-accepting lone pair on the nitrogen is occupied. The stronger EF strength may partially work to deprotonate the nitrogen and allow methylation to occur. This notion is supported by the differences in EF strength for sp 2 and sp 3 -targeting RNA/DNA MTases which have averages of -65.6 MV/cm and 52.9 MV/cm, respectively. This result indicates MTases have directly evolved their EF strengths to adjust to hybridization state of target atom.