Many analytical techniques such as spectroscopy (UV -Vis, FTIR, NMR, and MS) and chromatography (TLC, HPLC, and GC) have been applied in the development of methods for natural products (as bee pollen) identification and discrimination. FTIR spectroscopy may be an attractive choice among these techniques because it can meet the requirements of efficient analysis (i.e., simple to use, effective, and inexpensive) . While natural products can contain a wide variety of chemical components, it is often found that their FTIR spectra vary even for the same species . FTIR has already been commonly used and is a wellestablished method in various industries, including the herbal industry, for quality assurance . ATR-FTIR spectra include complex data describing the total chemical signal in a sample. Changes in band location and strength in the FTIR spectra can be related to changes in a sample's chemical composition. FTIR spectra may, therefore, be used to differentiate between closely related species although the composition of a chemical compound is not understood . Obvious benefits of applying FTIR to differentiate between different medicinal plants are not only successful and precise but also simple and non-separate.