Positive ion mode results. We investigated cell metabolites changed due to the omission of LN2 quenching by comparing the SCMS data obtained from cells in Group 1 vs. Group 2 as well as Groups 3 vs. Group 4. For the comparison of Group 1 and Group 2, abundances of 60 metabolites were significantly changed with 22 increased and 38 decreased metabolites (Figure ) (p-value < 0.05, FC > 1.5) . Pathway analysis did not identify any significantly impacted pathways (i.e., FDR > 0.05) (Table ). For the comparison of Group 3 and Group 4, abundances of 378