Elemental analysis of nitrogen for the prepared poly1 showed the introduction of 21 μmol g -1 of 1 into the resin architecture, and the successful linkage between 1 and EG40 via the phenoxy unit of the Tyr residue was confirmed by the absence of Tyr in the hydrolyzed solution (Table ). The nitrogen adsorption isotherm at 77 K showed retention of mesoporosity in poly1 after functionalization (Fig. and Table ). The affinity of OTA as an adsorbent for poly1 was investigated by determining the adsorption isotherms in the liquid phase using acetonitrile as the initial solvent. The temperature of the solution severely affected the thermodynamic parameters; therefore, the temperature of the system was carefully controlled during the investigation. When poly1 was dispersed in an acetonitrile solution of OTA, the emission from OTA decreased to a level close to zero within 2 h (Fig. ) following an intraparticle diffusion model (vide infra). Fig shows a sharp slope at extremely low concentrations (< 5 nM) of OTA for the poly1/OTA pair. In contrast, when a polymer without functionalization of 1 was used instead of poly1, almost no change was observed in the emission spectra. This accounts for spontaneous complexation between poly1 and OTA at the interfaces being thermodynamically favorable. The adsorption isotherms of poly1 at 273 K showed bi-Langmuir behavior in acetonitrile (Figs. and), and the analysis gave the association constants of 2.2 × 10 8 M -1 for the "stronger" mode (as K1) and 32 M -1 for the "weaker" mode (as K2) (Fig. ). The former value is one-order of magnitude larger than previously reported wellsophisticated systems using aptamer with K of 1.6 × 10 7 M -1 and polyaniline Langmuir-Blodgett film with K of 1.2 × 10 7 M - 1 . Thus, functionalized 1 (poly1) showed excellent binding to OTA in acetonitrile, but the details at the molecular level remain unclear. To further analyze the results, a van't Hoff plot was obtained using the binding constants for the homogeneous and heterogeneous adsorbent/adsorbate pairs in a narrow range of temperatures (Figs. and). Figs. 4ab shows the relationship between the logarithm of K and the reciprocal of temperature, which follows Eq. 2: