Given the above information, in this research, we are focusing on a comprehensive exploration of furan-based copolymers, investigating their potential as scaffolds in the dynamic field of biomedical engineering, emphasizing their ecological advantages and the integration of enzymatic catalysis for improved sustainability. Our focus extends to the electrospinning technique applied to poly(decamethylene furanoate-dilinoleic furanoate) (PDF-DLF) with 70 wt% PDF segments, introducing a groundbreaking avenue for the creation of sustainable biomaterials. This innovative approach aims to not only assess the biocompatibility and biodegradation profile of the manufactured material but also to evaluate its suitability for processing into nanofibers.