For successive tilt series acquired on the same crystals, overall R merge and I/sigma were plotted and compared over the lifetime of each crystal. For individual datasets from biotin and Zn(II)-histidine, which routinely were sufficiently complete to yield a structure solution, phases were retrieved by direct methods using either SHELXT or SHELXD , and refined in SHELXLE with addition of riding H-atoms and isotropic treatment of atomic displacement parameters (ADPs). The R1, wR2, and goodness of fit statistics after this treatment were recorded.