The structure of Cu(II)-bound SCP_Q111BpyAla was determined to 2.51 Å resolution (Figure and) and revealed a different orientation of the bipyridine moiety compared to that seen in the Cu(II)-bound SCP_Q111CBpy structure. Despite the lower resolution, the electron density for the BpyAla bipyridine rings, -helix 5 and the additional C-terminal amino acids (120-128) was very clear, indicative of a more rigid conformation for these parts of the protein.