We have used the Arpeggio package to quantify phosphorylation and mutationrelated differences. Arpeggio identifies and classifies interactions between atoms based on proximity and angles. We then summarised the counts for H-bond, ionic, aromatic, and van der Waals interactions between all residue pairs. Full details of this analysis are given in the methods section. Applying this analysis upon all 37,500 frames of a dataset facilitates quantitative measurement of significant changes in interactions between the different systems due to phosphorylation and mutations.