CSHNs exhibited the highest transfection efficiency and tumour suppression in tumour-bearing BALB/c nude mouse models. The results proved that the cationic surface charge of CSHNs was retained by the negatively charged cell surface due to the high affinity of the lipid envelope of the hybrid systems to the lipid-structured cell surface, and consequently, more drugs and genes entered the tumour cell via the fusion of CSHNs with the cell membrane. This research has shown that CSHNs can prolong the release of cancer molecules and genes more than other vectors and deliver them to tumour cells more efficiently in cancer treatment.