In this paper, we presented novel BODIPY dyes containing N-Nitroso moiety and showed the photoinduced generation of NO. We showed that for photodonors containing the phenyl ring (BODIPY-NOD-2,3,4,5) the maximal NO yield is observed for unsubstituted BODIPY, whereas boron alkylation and the introduction of the iodine atoms reduces the efficiency, although influence the singlet oxygen generation. For the isopropyl-containing photodonor BODIPY-NOD-1 the photorelease of NO was not detected. Using the developed NO photodonors, we demonstrated efficient light-dependent inhibition of platelet activation in vitro. We also show that some compounds could additionally generate singlet oxygen, which is promising for the photodynamic therapy. The presented compounds could be used in biological research and serve as the basis for the development of novel hybrid therapeutic methods.