The use of MnO2 in the development of electrodes intended for biosensors for the enzymatic determination of glucose is very promising. This material exhibits a high electrocatalytic activity of hydrogen peroxide decomposition, which is released during enzymatic oxidation of glucose. In this paper we show that manganese dioxide film properties depends on the composition and surface area of the material. The precipitate, electroplated under suitable conditions, shows electrocatalytic activity comparable with electrodes based on graphene and gold nanoparticles. The electrochemical formation of MnO2 films is easy, fast, reproducible and effective method. The film of manganese dioxide with better characteristics is formed electrochemically on the surface of the ITO in electrolyte with pH 1 by CV method and after this dried at 60 0 C. The maximum efficiency of electrode GOx/MnO2/ITO appears at modification with a solution of GOx enzymes with a concentration 2 mg/ml. The sensitivity of such an electrode is 117,8 μA/mmol cm 2 . The developed electrode can be used in glucose biosensors or similar systems.