As part of our ongoing effort to identify natural products with antimicrobial properties from the microbiota of marine egg masses, we have assembled over 800 bacterial strains from various marine egg masses. These strains, isolated primarily from moon snail egg collars collected in Florida and Puerto Rico, also contains bacterial strains from egg masses of various gastropods (Table ). A subset of these bacterial strains (140) was grown under three media conditions (1 L per condition) with hydrophobic resins that captured excreted bacterial secondary metabolites. Extracting the resins and subsequent fractionation by using flash chromatography (C8 resin) yielded eight chemically distinct fractions per bacterial strain. Individual fractions (810) were resuspended at 10 mg/mL in DMSO and arrayed into 96-well plates.