Considering the effect of crowding with time, the number of fragments left after the duration of the assay has the cognate equation derived as follows: being ≈ [ST] as well as [ST] being not ≫[ET] are relevant in line with the notion that the reactant stationary assumption (RSA) , standard quasi-steady-state assumption (sQSSA), and reverse QSSA (rQSSA) may be valid in such a scenario. As noted elsewhere, the literature review gave the impression, probably in error or due to the absence of self-evaluation, that the total mass of the product may be larger than the total mass of the reactant, in violation of mass conservation law. This motivated this study to derive alternate equations whose application should not violate the mass conservation law. The Preprint submitted to ChemRxiv, Jun 05, 2023