In order to extent iMME sampler capability, a handful of ARGs were investigated in the river Avon, UK. ARGs that were quantifiable were 16S rRNA, ermB, sul1, intI1, and blaTem (figure ). ARGs that were below the limits of quantification, via dPCR, were qnrS and blaCTX. The extraction efficiency of the kit was also investigated, via spiking of TaqMan™ Universal DNA Spike in Control into the lysis step of the DNA extraction kit. Results gave a consistent extraction (58 ± 2%) across duplicate runs, over triplicate biological replicates (figure ). The highest prevalence ARG in river water reported at 4.00E+06 ± 2.20E+06 copies/L was blaTem, which confers resistance to β-lactam antibiotics. This gene has been found in rivers worldwide (R. and has been identified as a potential indicator of anthropogenic AMR contamination (Narciso- . This has been attributed to reports of increases of blaTem after wastewater treatment processes . This is in comparison to reports of decreased amounts of ARGs, including ermB and tetW, after wastewater treatment processes . Another potential indicator of anthropogenic pollution has previously been identified as intI1, due to its association to a number of genes that confer resistance to AAs . It is common for intI1 to occur alongside sulphonamide resistance , hence correlations can be observed between the two in environmental samples. In this study, intI1 and sul1 were found in similar levels of 2.82E+05 ± 1.36E+05 copies/L and 1.46E+05 ± 7.87E+04 copies/L, respectively.