As shown in Fig. , plotting the affinities of the substrates for the NL6 amyloid (IC50) versus the reaction yields (%) confirmed the correlation of the two factors, except that the substrates underwent a directional change of the amine extension from the amyloid-biding site (2a and 2b). Again, the correlation of the binding affinity and yield is consistent with the mechanism of CASL, in which the amyloid-substrate complexation is key to promoting the reaction.