To conclude, this data confirms the anti-leukemic effect of 5b without inducing HSR. heat map, followed by unsupervised hierarchical clustering. The vertical axis of the dendrogram exemplifies the dissimilarity between clusters, whereas the color of the individual cell is related to its position along a log IC 50 (µM) gradient. b) Treatment of K562 cells with 5b and respective controls (AUY922 and NB) for 48 h resulted in the downregulation of BCR-ABL1+ and subsequently downstream signaling pathways including phosphorylated and unphosphorylated Stat5a, Crkl, Akt, S6 (mTOR), and cMyc. c) K562 cells were treated with the indicated (cytotoxic) concentration of 5b, NB, and AUY922 for 48 h, and later protein lysates were subjected to immunoblot analysis. As expected, 5b and NB did not induce expression of Hsp70, Hsp40, and Hsp27, whereas AUY922 leads to HSR induction. Hsp60 (primarily present in mitochondria) and PDI (endoplasmic reticulum) served as a control.