In summary, we have discovered a primitive reaction cascade of phosphonodithiolates formation and following hydrolysis. This reaction was constructed by using phosphate-based T3P as the chemical fuel and 4-methoxybenzenethiol as the precursor molecule. This formation of the P-S bond might imply of a possible chemistry and pathway for phosphate entering prebiotic metabolism. Moreover, this reaction cascade can further drive the formation of transient droplets. This type of non-equilibrium assemblies is distinct with the reported chemically fueled assemblies which are usually driven by chemically fueled activation/deactivation reaction cycles.