The column was filled with the stationary phase (upper phase) of the isocratic system and equilibrated with the lower phase at 10 ml/min and 1800 rpm. Then the CPC1 first fraction (252 mg) was dissolved in 3 ml of lower phase + 3 ml of upper phase and injected into the CPC column by a 20 ml loop. The mobile phase was pumped in ascending mode for 90 minutes. The column was extruded by switching the mode selection valve for 20 minutes at 20 ml/min. Fractions of 20 ml were collected over the elution step and combined according to their TLC profiles.