The radiosynthesis of [ 18 F]FSPG was conducted according to the methodology reported for the Scintomics HBIII automated synthesis module . The method proceeds via a two-step synthesis (S N 2 labelling and subsequent deprotection) followed by automated cartridge-based purification. Initial adaptation of the synthesis for the FASTlab was conducted using the same reaction conditions as previously reported (Table , Entry 1), using the cassette layout shown in Fig. . Production of [ 18 F]FSPG using these conditions was confirmed by radioHPLC analysis of the crude reaction mixture, although radiochemical conversion (RCC) was relatively low (23%). Unfortunately, elution of [ 18 F]FSPG from the MCX cartridges with PBS (10 mL) was unsuccessful and a manual elution of the radiotracer was required providing [ 18 F]FSPG of low radiochemical purity (87%). Furthermore, [ 18 F]FSPG was observed in the waste collection bottle, suggesting issues with the loading of the radiotracer on to the MCX cartridges or with subsequent washing steps.