The establishment of generic EME conditions primarily concerns the development of a suitable liquid membrane, and operational parameters such as sample composition and pH, acceptor pH, extraction voltage and time. The impact on extraction performance of these parameters was assessed using 31 acidic model analytes covering a wide range of hydrophobicity (-4.5 ≤ log P ≤ 6.1). Initially, the liquid membrane composition was considered and assessed using neat standards and human plasma samples. The final set of generic conditions was assessed using seven acceptance criteria, as defined below: