Subsequently, the total number of bioactivity data points as well as the number of variant bioactivity data points in the VEBD were calculated across families for each level. From these, the variant bioactivity percentage per family was calculated by dividing the amount of variant data by the amount of total data and multiplying the result by 100. Similarly, the novel variant bioactivity annotation percentage was calculated exclusively in ChEMBL data by dividing the number of bioactivity data points in potentially novel annotated variants (i.e. not previously defined in the ChEMBL 'mutatation' variable) by the total number of variant bioactivity data and multiplying the result by 100.