Though Jatropha can survive in dry lands too, but proper irrigation and application of fertilizer is required in initial years to obtain good yield . Cultivation of Jatropha provides the advantage of using wastelands and simultaneously producing useful biodiesel which can be used in today's vehicle fleet easily. 10-63 million hectare of such wasteland is reported to be available in India which can be used for Jatropha cultivation Many LCA studies published on Jatropha has not considered the combustion phase. However, from the life cycle perspective it is important to consider the complete life cycle of a product for doing an apple to apple comparison between Jatropha and the fossil fuel diesel. Only few studies are there where a complete Cradle to Grave comparison is done, and in these studies mostly GWP has been used as a single parameter to compare the biodiesel and diesel. Choosing GWP/CO2 emissions a single parameter for comparison give bias towards the biofuel as they are carbon neutral, therefore it is important to consider the other environmental impact categories as well. Study by Achten et al. has considered GWP, AP, EP, and Net Energy Ratio as the parameter to assess the biodiesel, however the system considered had less fertilizer inputs. Pandey et al. calculated the life cycle impacts of biodiesel production by taking average yield of 5 years as 4.52 ton of seeds per hectare, which included consideration of very high yields in fourth (7500 kg seeds/ha) and fifth (11250 kg seeds/ha) year. It is experienced that after fertilizer application and irrigation the average yields of Jatropha seeds over its life cycle are about 1.4-2 ton/ha .Montobibo et al. and Axelsson et al. reported the average yield even below 1000 kg/ha after interviewing the farmers. Study by Kumar et al. has also considered a very high value of seed yield which was 5.9-ton seeds/ha under irrigation condition and considered only GWP and net energy ratio. Study by also considered only the GWP and net energy ratio. This study shows the cradle to grave environmental impacts of Jatropha biodiesel in four major environmental impact categories and compare it with the reference fuel diesel: Global Warming Potential, Acidification Potential, Eutrophication Potential, and Human Toxicity Potential.