In vivo wound healing and toxicity studies: In all in vivo studies, the rats were kept in their houses for one week to acclimate them to the new conditions. During the studies, the cycle of 12 hours of light and 12 hours of darkness was maintained in the house, as well as the ambient temperature of 21-23 ºC and relative humidity of 50-60%. All principles of working with laboratory rats and methods were in accordance with the ethical standards and instructions of the Committee for Working with Laboratory Animals of Lorestan University of Medical Sciences (Ethical code: IR.LUMS.REC.1402.213). S. aureus-infected full thickness skin was employed to investigate the wound healing potential of hydrogels in a rat model. For this purpose, rats (male Sprague-Dawley rats, 180-220 g) were randomly divided into 4 groups (n =6) including wound alone without any treatment (Wound), the commercially-available dressing as a positive control (Tegaderm), Hydrogel (Gel) and drug loaded hydrogel (Gel-D).