Enlighten2 provides non-expert users easy access to efficient, automated MD simulations of proteinligand complexes, through a familiar interface and without assuming knowledge of setting up and running biomolecular simulations. The usage of Docker guarantees that the code runs and behaves identically on any machine and, apart from PyMOL and Docker, no user installation is required. It is therefore easy to use, also for classroom teaching or online workshops. In addition, by using Enlighten2 from the command-line or as a Python package, one can systematically run MD simulations for a set of ligands or enzyme variants as part of in silico workflows.