A flow battery utilizing DHAQ exhibits a temporal fade rate of ∼4.3%/day (measured in this study) under normal cycling conditions. When the electrochemical regeneration strategy is implemented, a fade rate of 0.38%/day is reported, which is over an order of magnitude improvement. However, this fade rate is still very fast, preventing DHAQ from being an attractive negolyte species for commercialization purposes despite its low predicted cost of manufacture. This study aims to explore the possibility of further reducing the fade rate. Thus, the effects of various factors that may affect the effectiveness of the electrochemical regeneration technique and the fade rate are investigated. These factors are the concentration of OH -, the cutoff voltage of the discharge step in normal cycling, the cutoff voltage of the regeneration step, and the period of the periodic regeneration events. Properly controlling these factors combined with restricting the negolyte SOC to 0-85% leads to a fade rate as low as 0.02%/day, which is over 200 times lower than that under standard cycling conditions. The findings and mechanistic insights obtained in this study are anticipated to improve the effectiveness of in-situ electrochemical regeneration for other electroactive species with finite lifetimes.