The POC application of this assay may, however, be limited due to its relative complexity. has reported a study of real-time detection of eaeA gene from STEC during the DNA amplification using RPA. The authors fabricated a gold nanogap electrode on silicon wafers, which was able to measure a change in impedance during the DNA amplification. The assay took only 30 minutes, including DNA extraction to achieve an LOD of 7 CFU/mL. These results demonstrate the highly promising step forward success and improvements in the field, however the real-world application of this study requires an easy-to-use approach such as microfluidics.