Alongside the EcDERA WT and variants we also screened the DERA from A. chlorophenolicus (AcDERAWT), which was recently shown to have the broadest donor substrate scope of a panel of DERAs , and fructose-6-phosphate aldolase (EcFSAWT). The panel of aldehyde donors included halogen (Cl)-substituted, two functional groups found in therapeutic oligonucleotides (OMe and MOE) and two typical protecting groups used in nucleoside chemistry (OBn and OTBDMS). These aldehydes were all screened with DL-glyceraldehyde-3-phosphate (DL-G3P, 1) as the acceptor.