QCM-D was used to test AB5 toxin binding to model glycocalyces. Representative data for STxB are shown in Figure , and demonstrate that STxB binds selectively, reversibly and in a dose dependent manner to Gb3 containing glycocalyx models (Figure ; 110 to 190 min; lines with square symbols). Comparatively little binding was seen on SAv-on-SLB coated surfaces lacking (HA-g-Gb3)-B (Figure ; lines with triangle symbols), and there was no response at all for glycocalyx models presenting Lac or Le x instead of Gb3 (Figure ). STxB also bound reversibly to (HA-g-Gb3)-H6 (Figure ), whereas there was no direct binding to the (Ni 2+ -NTA)3 SLB. These results demonstrated that biotin-anchored and H6-anchored mucin-like structures are suitable for interaction studies with this protein.