Bronchial epithelial tissue models were generated as described before. Briefly, 6.4 x 10 4 human bronchial fibroblasts were mixed with FBS and bovine collagen I (PureCol, Cedarlane), adjusted to neutral pH and added onto a 12 well cell culture insert. After 2 h of CO2-free incubation at 37 °C, expansion medium (PneumacultExPlus, STEMCELL) was added into the well and onto the insert. After additional 2 h of incubation at 37 °C, 5% CO2, the media on the insert was removed and 0.9 x 10 6 human bronchial epithelial cells were added. After 24 h, the media in the well was replaced by differentiation medium (Pneumacult ALI media, STEMCELL) and the media in the insert was removed. Then, every second day, media was changed. At day 16, 18 and 20, 1 µg/mL human plasma derived fibronectin (ThermoFisher) was added to trigger an inflammation. At day 18 and 20, dPGS was added to the treated wells. The models were harvested at day 21. For western blot analyses, the models were lyzed in a Tissuelyser II (Qiagen) with beads at 25 Hz for 5 minutes.