Amyloid pathology was further delineated using double staining with luminescent conjugated oligothiophenes and hyperspectral confocal imaging. The LCOs quadro-formyl thiophene acetic acid (qFTAA) and heptamer-formyl thiophene acetic acid (hFTAA) were used for staining polymorphic plaques . Following MALDI analysis, tissues were rinsed in absolute EtOH for 60 s, fixed in absolute EtOH (Cat # V002075; Sigma Aldrich) at -20°C for 8 min, 70% EtOH at -20°C for 30 s, 70% EtOH at RT for 30 s, and rinsed for 5 min in PBS (Cat.#: P9416, Sigma Aldrich) both prior and after staining. For fluorescent amyloid staining, tissues were incubated with qFTAA (3 μM in PBS) and hFTAA (1.5 μM in PBS) in a humidification chamber for 30 min at RT. Tissues were mounted with Prolong Gold antifade reagent (Thermo Fisher Scientific) and dried for 2h at RT. Hyperspectral imaging was performed on ELYRA PS.1 / LSM 780 laser-scanning microscopy equipped with a 32-Channel GaAsP spectral detector (Zeiss). The objective used was Plan-Apochromat 20x/0.8 (WD= 0.55 mm), ∞/0.17. The excitation wavelength for LCOs was 457 nm. The emission spectra were acquired in the range of 405 to 750 nm as described before .