Square-lattice arrangement is an alternative approach to building three-dimensional structures made by using a 90° offset between crossovers. Fig. shows a 236 ± 25 nm long three-fold bundle with up to two neighbours per folded segment, assembled with a 3246 bp scaffold and 40 staples. The staples have both 15 nt and 30 nt recognition domains and retain a crossover density of two crossovers in each 60R21N section, of which it repeats ten across its length.