Cardiovascular diseases continue to be the global leading cause of death overall and have a significant contribution to health loss and economic burden . Therefore, innovative detection tools are required for accurate and timely disease detection. Recently, Lee et al. have demonstrated that aptamers can be used for the detection of cardiac disease . In the current research, we have performed a computational analysis of how these aptamers can be used as a cardiac disease biomarker. We hypothesize that the high specificity of these aptamers towards the TNF-α protein is due to a specific binding site on the surface of this soluble protein.