The hydrogen bonds between the guide and target RNA base pairs remain stable in all tested force fields (Table and Figure ). From these force fields, OPLS4 and ff19SB+OL3 match the crystal structure hydrogen bond count. In all the nonpolarizable force fields, there are on average more observed hydrogen bonds between the protein and the RNA than in the crystal structure. In the polarizable simulations, there are less hydrogen bonds than in the crystal structure. The complexes in polarizable conditions are not as compact as in the nonpolarizable simulations. The complexes open more in these simulations, which increases the distances of the hydrogen bonds beyond our cutoff distance and lowers the number of hydrogen bonds.