Moreover, it was possible to separate broad particle size distributions that contain agglomerates and samples containing multiple particle populations-a feature superior to common characterization methods such as batch DLS, AFM or SEM. Microscopic methods (i.e., AFM and SEM) are still very valuable to further elucidate the shape and morphology of particles. However, for multimodal particle size distributions, attention must be given to data fitting when processing MALS signals-as the automatic fitting performed by the software may not always be accurate and requires verification by the user. The bore diameter of the installed flow cell of the MALLS785nm plays an important role for determining Rh from DLS. Using a flow cell with a wide bore diameter allowed to accurately determine the Rh and shape factor  of LNPs. Moreover, laser wavelengths of 660 nm or 785 nm are both suitable to determine the Rg.