Besides a missing link on the suitable enzyme that performs the lactylation process, a debate is pertinent on the existence of an enzyme that converts lactate to lactyl-CoA. By using molecular docking studies, we have screened various potential enzymes as potential players that may catalyze the formation of lactyl-CoA from the lactate (data not shown). During the screening, a key enzyme is predicated as acetyl-CoA synthetase that may potentially prefer the lactate as a substrate during the formation of lactyl-CoA that is similar to the acetyl-CoA formation from acetate (Figure ). Data obtained from DSV3 predicted the almost similar binding pockets with hydrogen bonds between acetate (GLN415, ARG515, ARG526) and lactate TRP413, TRP414, GLN415, ARG515, ASN521) for acetyl-CoA synthetase (Figure and Table ).