We analyzed the influence of the mother droplet size, the fraction of long PSS, and its length on the division mechanism. First, we varied the size of the mother droplet by analyzing different microreactor sizes. An increased microreactor volume increases the droplet material and, thus, a larger coacervate droplet after fusion (Fig. ). We found that droplets smaller than roughly 145 μm 3 , i.e., those with a 3.3. µm radius showed no division. From there on, the number of offspring fragments increased with the size of the initial coacervate droplet until it leveled off at, on average, 23 offspring (Fig. ). Increasing the coacervate droplet size also increased the variability in the offspring number. We hypothesize that critical droplet size is needed to accumulate sufficient long PSS to produce at least two speckles. Besides, larger droplets lead to more speckles.