Overall, this combination of C2 and C3 beads provides a better hydrophobicity landscape than the Martini 2 model, performing better in both octanol/water (within 15% of experimental reference, Figure ) and hexadecane/water (within 10% of the GROMOS 54a7 theoretical reference, Figure ) partitions. As seen in the following sections, this improved description of cholesterol hydrophobicity did not negatively affect the cholesterol-induced phase separation in ternary lipid bilayer systems, thus showing a good compromise between the two properties.