. To confirm, the "blobs" we were seeing in the confocal imaging are the cargo conjugate surrounding the cells, we performed super-resolution imaging (Figure ). Here, we prepared a new cargo conjugated with Alexa Fluor 647 (hereafter, referred to as AF647) for three-dimensional single-molecule super-resolution microscopy using a biplane configuration. With the inferred axial position of the localized single molecules encoded in color, we observe the three-dimensional distribution of AF647 labeled cargo on the cell surface confirming our hypothesis that the polymer conjugate binds preferentially towards the cell surface membrane.