To demonstrate the utility of the purine nucleoside salvage pathway enzymes for synthesizing nucleosides functionalized at the 2′-position the EcDERAF76A variant was combined with EcPPMWT and EcPNPWT. This allowed the synthesis of the ribonucleosides adenosine and guanine as well as 2′-Me and 2′-F adenosine analogues. These nucleoside analogues can be generated in a one pot reaction starting from relatively simple starting materials. By changing the aldolase used from EcDERAF76A to EcFSAWT, stereochemical control can be imparted at the 2′-position allowing for the synthesis of the nucleoside analogue vidarabine.