First, this topical antibiotic suffers from low oral bioavailability (30-40% at a single dose of 200-400 mg), so its LPN formulations could mitigate this complication. Second, the LPN formulation would allow prolonged administration of this topical antibiotic while minimizing its use in an infected area. Thus, the patient does not face the inconvenience of frequent application of the drug. To optimize the formulations, the researchers created some quadratic equations using software that related the per cent entrapment efficiency (% EE), particle size (PS), and per cent cumulative drug release (% CDR) to the response. From this model, the ideal ratio of lipids to polymers was easily derived, which also facilitated the interpretation of the overall results. In conclusion, the results show that the nanocarriers exhibit significant activity against both Staphylococcus aureus and Pseudomonas aeruginosa. Thus, it can be deduced from this study that optimization is one way to improve the manufacturing process of LPN formulations and enhance their performance .