In terms of material selection for vascular scaffold fabrication, it is essential to prioritize ideal biological properties, including favorable cell-material and tissue-material interactions, alongside considerations of biodegradability and mechanical strength , . Biodegradable natural and synthetic polymers, such as collagen, elastin, poly(caprolactone) (PCL), chitosan, polyurethane (PU), and poly(lactic acid) (PLA), among others, have been extensively explored for their ability to be tailored to achieve desired properties , , . In our group, new series of poly(butylene succinate)(PBS)-based copolymers have been developed using dimer linoleic acid (DLA-OH), which has enabled the achievement of a wide range of mechanical properties of poly(butylene succinate-dilinoleic succinate)(PBS-DLS) materials from stiff to elastic . Additionally, polyethylene glycol (PEG) modification to PBS-DLS copolymers, have been tailoring their biodegradation rate by improving surface wettability to facilitate hydrolysis, also showing excellent cytotoxicity, thus making them suitable for biomedical applications . These copolymers exhibited excellent melt and solution processibility, making them highly suitable for electrospinning and extrusion 3D printing techniques, allowing for the transformation into nanofibers with diameters ranging from 400 to 600 nm and demonstrating good printability, enabling the fabrication of structures with up to 400 layers stacked atop each other . Furthermore, endothelialization of the lumen is vital for vascular graft success, but achieving it remains a significant challenge in vascular tissue engineering . Endothelial cells are anchored to the basement membrane, which includes collagen, laminin, and proteoglycan, playing a crucial role in regulating permeability, shear stress transduction, and angiogenesis. Coated materials like collagen and gelatin can promote endothelialization, enhancing the integration and function of engineered vascular tissues . Considering all the advantageous copolymer's characteristic and demands for multi-layer scaffolds for vascular prosthesis, we used PBS-DLS-PEG copolymers for integrating 3D printing and template-assisted electrospinning to produce a complex structure mimicking artery (5mm diameter). Customized copolymers were employed, to fabricate tubular 3D printed porous structure serving as a template for electrospinning within a bespoke collector. This facilitated the creation of tunica media and externa layers, while gelatin coating simulated the tunica intima. Notably, electrospun fibers comprised nanocomposites of copolymers and cellulose nanocrystals, enhancing mechanical properties. Morphological and mechanical evaluations, including tensile strength, burst pressure, liquid leakage, and compliance, were conducted to provide insights into scaffold performance for potential tissue engineering applications.