The first fragment growing strategy for the PDB-ID 2W6M, was developed to grow to PDB-ID 2W6N, (Figure . A). The main difference was in gaining interaction with the Leu-A-278 (Accessible-category) residue that was identified as highly accessible with moderate interaction energy in the apo-structure FMOPhore scanning and Val-A-171 (non-hotspot category). Although this growing strategy led to a better interaction energy and IC50 value, however the experimental LE shows little change for the lead compound, which suggests that this fragment growing strategy could have been improved if targeted to other hotspot categories than the non-hotspot residues.