A key application of AgNPs is their role in Surface-enhanced Raman scattering (SERS). AgNPs allows the detection and identification of single molecules by enhancing the efficiency of SERS by as much as 10 14 to 10 15 folds . Due to these unique properties AgNPs have found its application in various fields, particularly sensing and imaging technologies. They are utilized in detecting DNA, where their plasmonic properties enable high sensitivity . Additionally, AgNPs are employed in selective colorimetric sensing for biomolecules such as Cysteine , monitoring Purine nucleosides phosphorylase activity , and detecting environmental contaminants like Mercury .