The accuracy of the assay was analyzed with colored-solutions. Therefore, the kinase reaction PCR plate and the assay plate were prepared independently by the liquid-handling station. Then, the absorbance between 300 and 700 nm were recorded and the differences in the spectra were evaluated. For the kinase reaction plate, the dyes blue (water), pink (buffer), green (substrate) and black (enzyme) were used. From the PCR plate, 100 µL were manually transferred to an assay plate for analysis. For the assay plate, blue (water), pink (kinase reaction) and black (Kinase-Glo reagent) were applied.