The results confirm the role of tether length to have an independent role in determining the outcome of adsorption events. Furthermore, the qualitatively similar trends observed for the adsorption of AuNP and NAv onto APT and BPT respectively indicates the impact of the tether length on the adsorption events to be generic and independent of the nature of interactions that drive the binding events. However, the impact of tether length on the enhancement of adsorption of AuNPs onto NH2-(EG)n-SH was found to be more pronounced as compared to NAv adsorption to biotin-(EG)n-SH. This difference could potentially arise due to the limitation of 4 biotin binding sites per NAv as compared to the abundance of binding sites on surface of