Sample preparation is an important step in LC-MS analysis of juices and wine due to significant matrix effects and the presence of isobaric interferences . Various approaches have been described, including , the quick easy cheap effective rugged and safe method (QuEChERS), liquid-liquid extraction (LLE), solid phase extraction (SPE), and solid phase microextraction (SPME). Well-established procedures such as QuEChERS are time-consuming, errorprone, tedious to automate, and generate significant waste. Column-switching or online SPE , which was primarily developed for bioanalysis offers an attractive alternative for the automated analysis of liquid samples and has been successfully applied for the determination of pesticides in surface waters , drinking and ground waters , wine and juices . J.D. describe the analysis of pesticides in wine samples, including neonicotinoids and pesticide metabolites, using direct large volume injection (DI-LVI). Sample dilution of the wine with water was mandatory to avoid analyte loss and maintain good chromatographic peak shape.