Initial coordinates for wild-type (H/F) BurPL, LCC, and TfCut were obtained from the crystal structures deposited in the Protein Data Bank 28 under PDB IDs: 7CWQ, 12 4EB0, and 5ZOA, respectively. For IsPETase, the initial structure corresponded to the recently solved structure of the single-point mutant S214H, PDB ID: 7CY0, where the serine residue in S/I was replaced by the histidine that is residue strictly conserved in other PETases. The S/I versions of BurPL, TfCut, and LCC, as well as the S/I and H/F variants of IsPETase, were generated using the Mutagenesis tool in PyMOL v. 2.4.2. Both IsPETase and BurPL harbor an active site (C203-C239 for IsPETase, C333-C370 for BurPL) and a C-terminal (C273-C289 for IsPETase, C404-C424 for BurPL) disulfide bond.