Global MS data parameters utilized on the Orbitrap Exploris 240 were as follows: Intact protein was selected for application mode, low pressure was selected for pressure mode, liquid chromatography was selected for infusion mode, the expected peak width was 10 s, advanced peak determination was selected, the default charge state was 35 and internal mass calibration was off. The ion source properties registered the CE source as an ESI ion source with a static spray voltage and a positive ion capillary voltage of 0 V was used. A static gas mode was utilized with the sheath gas at 2 arbitrary units (au), auxiliary gas at 0 au, and a sweep gas at 0 au. The ion transfer tube temperature was set at 200°C. The MS scan parameters used are also as follows: Full scan MS1 analysis was performed in positive ion mode with a scan range of m/z 740 -2,000. Samples were analysed with an Orbitrap resolution of 15,000 (15K) at m/z 200, the RF lens was set at 125%, the normalized AGC target was 50%, the maximum injection time was 200 ms, and the number of microscans was set to 2. Data was collected in profile mode. To assist in desolvation, the source fragmentation parameter was set to 35 V.