The antioxidant activity of the peptides was assessed using DPPH. Peptide solutions (0.1 or 8 mM) in phosphate buffer (pH 7.4, 25 mM) were mixed with DPPH (160 mg/L) Ascorbic acid was used as a control. The mixtures were left for 30 min in a dark place before measuring the absorbance of the DPPH radical at 517 nm using a Biotek Synergy H1 plate reader (Lumitron). The experiment was conducted in triplicate and the results were compared using one-way ANOVA test with Tukey-Kramer post hoc analysis.