Figure shows the components of the cartridge design with the multilayer configuration using alternating plexiglass plates, spacers, and Teflon based gaskets to create the hermetic seal in between the plates. All the layers were held together and compressed using nuts and bolts through the holes around the outer perimeter of the cartridge. The cartridge before any sample has been loaded. 2. The samples are loaded onto the TLC plate using a needle to penetrate through the rubber septum maintaining the hermetic seal before and after loading. There are 6 rubber septa allowing 6 samples to be loaded which include the calibration curve samples and the patient sample.