A limitation of UmetaFlow is the requirements for basic programming skills in order to implement and adapt the Snakemake workflow, as well as access to a server, cluster or cloud environment for processing very large datasets, due to storage requirements that exceed specifications of a common PC. However, the user can delete all the interim files at the end of a run. The most computationally demanding process in the workflow is SIRIUS step, which is optional. Nevertheless, for small(er) datasets, the user can run the workflow successfully using a common PC, such like the one used for benchmarking, either through the Snakemake workflow, the Jupyter Notebooks, or the webbased GUI.