The simulation output was calibrated against experimental data from monomer-only runs. As the total protein concentration was constant (20 µM), and only the leading edge of the peak was used as reference data (avoiding confounding effects from adsorption of fibrils to the capillary walls), a separate calibration factor for fibrils was computed. The hydrodynamic radius of the monomer was experimentally determined using the FIDA 1 instrument, and its diffusion coefficient used as derived Eq. 9.: 𝑦 = 𝑦 0 + 𝐴 (1 + 𝑒𝑥𝑝(-𝑘(𝑡 -𝑡 0.5 ))) ⁄