We find that single-conformer partial charge generation methods, specifically AmberTools AM1-BCC and single-conformer OpenEye AM1-BCC, exhibit more partial charge variability (as judged by either metric) on average than OpenEye AM1-BCC ELF10 charges. The AM1-BCC ELF10 charge is designed to be relatively conformer-independent, but it requires at least 10 conformers and recommends a set of at least 500 conformers as input. For our AM1-BCC ELF10 charge generation, we use the recommended 500 conformers. (Figure ). Again using both metrics, single-conformer OpenEye AM1-BCC is the most variable method, followed by AmberTools AM1-BCC and OpenEye AM1-BCC ELF10. OpenFF's NAGL has 0 variability across all molecules using both metrics as expected, since NAGL is a conformer-independent method, and thus produces the same partial charges irrespective of the input conformer.