In addition to the number of protons, the pKa values are also important as they indicate the location of the inflection point that will likely influence the system response of the binary mixture. Systems containing two poly-protic buffers (e.g. citrate and phosphate) tend to require fewer iterations compared to systems containing monoprotic buffers such as ammonium or acetate. Given the variety of the tested buffer chemicals, we believe that a wide variety of buffer systems can be represented using the dataset, i.e. the strategy should be applicable to samples containing other pH-sensitive chemicals which are not directly represented in this study. for indexed buffer system positions.