A control experiment involving titration of psDiMan into the binding buffer (Figure (B)) was also recorded to measure the heat of dilution, which was then subtracted from the psDiMan-DC-SIGN binding titration to obtain the binding enthalpy change between psDiMan and DC-SIGN as shown in Figure (A). The standard MicroCal one set of sites model was used for fitting the plot of enthalpy changes, during which N (number of binding sites) was fixed at 4 as there are 4 CRDs on each DC-SIGN. The following binding thermodynamic parameters were obtained: Kd = 1.1 ± 0.3 mM, ∆H o = -23.4 ± 2.7 kJ mol -1 , ∆G o = -17.0 kJ mol -1 and ∆S o = -21.5 J K -1 mol -1 .