The membrane dimensions were investigated through APL and membrane thickness estimations. The APL was derived either from the box size in x-and y-directions Only MMG-1 has been reported to assemble into fully interdigitated membranes below 25°C, whereas MMG-6 has been proposed to form non-interdigitated structures at the temperature used for the simulations. 11 In contrast to these experimental results, no apparent difference was observed from the mass density profiles (Figure ). Both visualizations and the number density profile histograms show that the tails of MMG-1 and MMG-6 extend into the opposing leaflet, forming partially interdigitated regions (Figures ). The degree of partial interdigitation is slightly greater for MMG-6, which is evident from the larger leaflet overlap. However, partial interdigitation is a phenomenon encountered with lipids both in the gel and the disordered phases and does not necessarily predict the formation of a fully interdigitated phase. MMG analogs display differences in tail order, tilt angles, and conformations