Here following a similar strategy in designing surface chemical contrast nanopatterns for selective binding, a good passivation background against streptavidin is achieved with a PP1 brush monolayer that has minimal non-specific binding of streptavidin, and surface regions for specific binding of streptavidin is modified by biotin-PP1 brushes. This strategy successfully yields surface chemical contrast nanopatterns (biotin-PP1-PP1) with excellent selective immobilization of streptavidin on circular patches at length scales defined by electron-beam lithography (Figure ).