Electrochemical Impedance Spectroscopy (EIS) has demonstrated its effectiveness in probing nano-bipolar electrodes (nano-BPEs), making it a promising candidate for this application. Initial studies, conducted in the absence of cells, involved measuring the impedance BES before and after wire growth. A potentiostat was connected to two BPEs (illustrated in Figure ), and EIS measurements were performed in galvanostatic mode during MW growth, resulting in Bode plots (Figure ). EIS was used to assess the overall BES impedance before and after MW growth. Results (Figure ) consistently showed that MW growth led to a reduction in the BES's impedance. This confirms the ability of EIS to effectively signal MW growth, suggesting its potential for adaptation in systems with biological components.