To evaluate the potential toxic effects of a copolymer's components (extract of the materials) on cells, the indirect cytotoxicity study was carried out according to ISO10993-5, using extract materials and L292 cell line . Briefly, 96-well plate were used to culture 10 x 103 of L292 cells in each well using complete growth medium (Dulbecco's Modified Eagle Medium (DMEM), containing 10% Fetal Bovine Serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin). The cells were kept in CO2 incubator over 24 hours at 37 °C. In parallel, the samples films (0.5 mm of thickness) were prepared with 3 cm2 of surface area, cut into small pieces, sterilized using ultraviolet (UV) light (each side 30 min) and placed in 24-wel plate. They were incubated in CO2 incubator over 24 hours at 37 °C in 1ml of complete growth medium. Commercial, non-toxic poly(ε-caprolactone) (PCL, Capa™ 6430) were used as negative control, nitrile glove (Mercator Nitrylex Classic, Kraków, Poland) was used as positive control, and media in empty well as sham. After 24 h, the cell culture medium was aspirated from the plate and replaced with 100 μL of extract medium from a given sample (5 technical repetition: 3 samples per material). The plates were then returned to the cell culture incubator for another 24 h of culture. 20 μL of resazurin with a concentration of 0.15 milligrams per milliliter in phosphate-buffered saline were added to each well, including the control wells containing just the media, and the samples were incubated for an additional 4 hours at 37 degrees Celsius. The fluorescence intensity was then determined using a Biotek Synergy HTX multi-functional plate reader (Biotek and is located in Winooski, Vermont, USA), with a 540 nm excitation wavelength and a 590 nm emission wavelength.