Tzs were synthesized via a Pinner-like synthesis. Briefly, required nitriles were either commercially available or synthesized. Corresponding Tzs were obtained using a sulfur catalyzed synthesis strategy reported by Qu et al.. Rate constants were determined with TCO by pseudo-first-order measurements in DPBS at 37 °C by stopped-flow spectrophotometry. All tested Tzs displayed rate constant (k2) values above 70,000 M -1 s -1 (SI).