tized by k (Table ). Examination of this data revealed that, except for 226 the first and the ninth entries that were, respectively, an isotopologue We next shifted from this tabulated metabolite level view of the data to the classical graph-based organization of the MS/MS data. During the examination of this expert knowledge-informed MN, our attention was attracted by molecular families (MFs) A and B (Fig. ). In MF A, the node at m/z 631.3986, ranked by k as 525/754 (i.e., the least unknown in this MF) showed a t g,i (Tanimoto between GNPS and ISDB-LOTUS structural annotations) value of 1 and was annotated as pleiomutine, a well-known compound reported from P. mutica . This first case highlights the interest of performing a GNPS library search with a low threshold score to catch an annotation that can be further compared to an in silico annotation using a strict library search such as usually performed with ISDB-LOTUS. Beyond the aggregation value proposed by MS2DECIDE, practitioners have access to important indicators that can assist them in their decision process.