Solution data established excellent water solubility, sensitivity, and high selectivity for ONOO - and ATP using their respective detection emission profiles. As a preliminary proof-of-concept study for cellular applications, changes in ATP and ONOO -associated with acetaminophen (APAP) were evaluated. This model was chosen due to the importance of pre-clinical tools for the screening of drug-induced liver injury (DILI) associated with drugs like APAP are crucial for drug development. Moreover, the model was selected because APAP can result in a depletion of ATP and an increase in the levels of ONOO -(Scheme S1). It is important to note, we first disclosed our probe ATP-LW as a ChemRxiv preprint, however, we felt that the paper could be substantially improved with the addition of biological experiments and so submission for publication of that paper was delayed. In order, to carry out those biological studies, we needed to resynthesize ATP-LW to fully evaluate the biological properties of our probe, however, the Covid-19 pandemic severely hindered our research progress. During this delay the group of Tian et al. reported a somewhat similar structure for the simultaneous detection of ATP and H2O2. Pointedly, Merck recognized the potential of ATP-LW and have made it commercially available. As such, this publication serves to fully highlight the utility of our now commercial ONOO -and ATP fluorescent probe ATP-LW. Scheme 1. Chemical Structure of ATP-LW and its fluorescence 'turn on' mechanism in the presence of ONOO -, ATP and ONOO -/ATP. The addition of ONOO -leads to formation of compound NA-OH, with maximum emission at 562 nm excited at 450 nm and maximum at 568 nm excited at 488 nm in PBS buffer solution (10 mM, V/V, EtOH/H2O = 1/99, pH = 7.40). The presence of ATP affords the product Rh-Bpin, with maximum emission at 587 nm when excited at 520 nm in PBS buffer solution (10 mM, V/V, EtOH/H2O = 1/99, pH = 7.40).