To investigate the influence of low temperature storage on cell metabolites' profiles, we performed the same comparison (Group 1 vs. Group 3 and Group 2 vs. Group 4). The comparison between Group 1 and Group 3 revealed 86 increased and 141 decreased metabolites (Figure ). Pathway analysis demonstrated that multiple pathways were significantly affected (Figure , Table ): arachidonic acid metabolism, arginine and proline metabolism, linoleic acid metabolism, D-Amino acid metabolism, valine, leucine and isoleucine biosynthesis, pantothenate and CoA biosynthesis, alanine, aspartate and glutamate metabolism, and Galactose metabolism. In the comparison between Group 2 and Group 4, 87 metabolites were increased and 187 were decreased (Figure ). Three metabolic pathways were significantly impacted (Figure , Table ): arachidonic acid metabolism, valine, leucine and isoleucine biosynthesis, and galactose metabolism.