Consequently, MBTPA-Tz aggregates were proven to selectively target and visualize biological targets labeled with a strained alkyne group. It is noteworthy that it shows better photo-bleaching stability than any commercially available bio probes used in this study (Figure , Supporting Information). These compelling findings provide substantial evidence that the MBTPA-Tz nanoprobe can effectively serve as a versatile biological imaging tool, as demonstrated by its exceptional multi-organelle targeting capabilities.