We used the Martinize2 tool in the vermouth v0.9.1 4 Python 3 package for coarse-graining of the atomistic protein structures (for the ClC homodimer we used vermouth v0.9.3 4 ) and controled the DSSP restraints with the -ss flag. The DSSP sequence was found using the DSSP v2.2.0 7 implementation in MDtraj v1.9.7. Additionally, we used the -scfix flag to restrain the side chain dihedrals. OLIVES systems were set up based on the coarse-grained protein output of Martinize2