It is important to note that, as per previous observations, NPs are found in higher dilution medicine samples, but the origin of nanoparticle formation is not reported so far. Based on the current observation, we can clearly see that the nanocrystalline Au NPs are formed during the trituration process itself. This highlights a unique observation that the initial preparation (starting material) for the high homeopathic dilution is very much different from the other conventional medicinal preparation, which was never noticed so far, leading to erroneous conclusions.