As shown in Figure , MW growth was not possible at 10V for any frequency, similarly, MW growth was not possible at 1 kHz. The exact reason for this is unclear and requires further investigation. Figure insets I-VI show varying frequencies producing observably different MWs. At frequencies >100Hz, broken MWs form that are 0.5-1 mm; these MWs form when using 100V, or by applying 100V for 2 seconds before lowering to 50 V. Higher Frequencies (10 kHz) initially produce MWs with a wider diameter, with large amounts of amorphous growth. Wire thickness gradually decreases becoming less amorphous to ~1 mm at 1 MHz. This proportionality of decreasing diameter at higher frequencies is comparable to AC MW growth using other materials, although wire thickness is magnitudes larger. Ten and 100 kHz allowed for MWs to be grown at 50 V when applying 2 seconds of 100 V to initiate MW growth. Furthermore, 1 MHz induced growing MWs at 50 V, 100 kHz growing MWs between 60 and 70 V, and 10 kHz growing MWs between 80 and 90 V. One of the critical advancements ultimately needed to facilitate in vivo MW growth is a method to non-invasively determine if growth has occurred. Visualising wire growth within 3D cell cultures is challenging, and these difficulties will likely be amplified in tissue samples and animal models. Therefore, a wireless monitoring technique for wire growth would be highly valuable.