The general insertion of the Martini 3 cholesterol model roughly follows the behavior of the previous Martini 2 model, with the center cholesterol population in unsaturated bilayers being slightly more prominent in the new model (Figure ). For the polyunsaturated DAPC membrane, the Martini 3 model performs slightly better than in Martini 2, where the center population is expected to be dominant 103 ; however, it is difficult to say whether it is overestimated in the remaining unsaturated bilayers with the existing experimental data. This effect can also be measured via flip-flop rates, which increase with decreasing saturation (Figure ). The Martini 3 flip-flop rates observed were slightly increased in unsaturated bilayers when compared to Martini 2, hinting at a lower energy barrier for translocation. In saturated membrane systems, Martini 3 cholesterol flip-flop rates are in agreement with both Martini 2 and reported rates calculated from transmembrane free-energy profiles of cholesterol 105 .