Yang et al. reported a 3D microfluidic paper-based chip and a smart detection module have been proposed as a method for determining the concentration of albumin (ALB) in human whole blood samples. The paper-based chip is fabricated with a reaction area implanted with bromocresol green (BCG) reagent and stored in a nitrogen (N2) environment at a temperature of -15 °C until needed for use. To detect the ALB concentration, 10 μl of whole blood is placed on the entrance area of the chip, and the plasma within the sample diffuses through a separation channel into the reaction zone. A reaction between the ALB in the plasma and the BCG reagent is induced by heating the chip at 37 °C for 6 min, after which the ALB concentration is detected using a colorimetry technique implemented on a cell phone in the form of a self-written app. Experimental results obtained using control samples with ALB concentrations in the range of 3.68 ∼ 81.0 g/L have shown that the G + B intensity (Y) of the reaction image is related to the ALB concentration (X) as Y = -0.995 X + 327.26 with a high correlation coefficient of R2 = 0.9958. The proposed detection system has been shown to provide reliable results for practical ALB determination purposes, as demonstrated by the consistent results obtained for 40 pure ALB samples and 30 whole blood samples using a traditional spectrophotometry method (R2 = 0.9837 and R2 = 0.9968, respectively). In summary, the proposed system offers a simple, inexpensive, and reliable alternative to traditional bench top methods for performing the ALB concentration mensuration of clinical blood samples, making it a valuable tool for clinical applications.