The α-TP-loaded PBA-DLA NPs were prepared via the nanoprecipitation method as shown in Figure . Briefly, the PBA-DLA (10 mg/ml) and α-TP with increasing concentrations (2.5, 5, and 10 mg/ml) were dissolved in 1 ml of acetone, which was dropped slowly into the aqueous solution of the PLUR stabilizer (3 ml, 0.05 mg/ml) under room temperature using magnetic stirring with 700 rpm. Next, the dispersion was centrifugated at 15000 rcf (t = 15 min, T = 4 °C). After the supernatant was removed, the NPs were redispersed in deionized water and the washing methods were repeated three times. The obtained NPs samples were frozen in -20 °C and lyophilized (Christ Alpha 1-2 LDplus apparatus).