Consequently, the reaction time was extended to 3 days, yet even after this period, the reaction remained incomplete. (results are presented in Fig. SI-17). This result suggests that the enzymes exhibit greater selectivity for aliphatic polyesters, as the aliphatic polyester was hydrolyzed in a much shorter time frame. (in less than 1 for both enzymes). SEC-UV analysis revealed a higher decrease of polymer area for PES 4 with respect to PES3 (PES3 24% vs 16%, PES4 20% vs 33% in 3 days with LCC WCCG and Tc_Cut2NV WCCG respectively). We suggest that the lower hydrolysis of PES3 is associated with the higher molecular weight of the polymer, which increases the Tg and reduces its molecular accessibility.