A similar procedure was performed to build a larger LNP with a diameter of approximately 40 nm. In this case, after step 1, the relaxed inverse hexagonal system was replicated in step 2 to generate an LNP with 44 channels in total, followed by the same procedures described in steps 3 and 4. For the 'bleb' structure, steps 1 and 2 were replaced by the construction of two vesicle-like compartments using TS2CG and Polyply. One compartment contained water, ions, and a long 1,000 ssRNA chain, while the other contained neutral ionizable lipids and cholesterol. After construction, both compartments were relaxed and the outer coating was generated, as described in Steps 3 and 4. After construction, the resulting LNP particles are then solvated and the desired ions are added using insane and GROMACS, followed by 5 steps of relaxation, as detailed in the Jupyter notebooks.