Before analyzing the simulation results, it is instructive to first look at the expected assembly behavior of NPs based on their free energy landscape 𝑈 )* , which has a simple 3D form for a system of three particles, calculated as the sum of three pairwise two-body interactions and a single three-body interaction (see Eq. 1). This energy landscape is plotted in Fig. with respect to 𝑑 4! and 𝑑 4% = 𝑑 !% and in Fig. with respect to 𝑑 4% and 𝑑 !% (𝑑 4! is fixed at 6.12𝜎).