Overall, the recognition and binding behavior observed with these representative proteins show that the Martini 3 cholesterol model is correctly interacting with proteins and replicating both experimental and theoretical references. For users comparing the results obtained with the previous Martini 2 model with the current Martini 3 cholesterol, it is important to account for the overall stickiness of the Martini 2 model 43 . Molecules in Martini 2 were found to be too hydrophobic or self-interact too strongly, particularly when mappings finer than 4-to-1 were employed. This impacted both proteins and possibly cholesterol, and as such, it is to be expected that Martini 3 protein-cholesterol interactions are more transient in nature than the Martini 2 counterparts. Indeed the cholesterol residence times calculated for our test cases were in the 80-100 nanosecond timescale, while in Martini 2 the typical cholesterol residence time would likely be in the microsecond timescale.