It is noted that the above calculations and analyses cannot be accurate. The ions are treated as point charges, the Pauling repulsion force is not considered, the distribution of energy in different degrees of freedom of motion is ignored, the interaction of species shown in an equation with those not in the equation is not considered, and the ion pairs are assumed completely stable during desorption and ionization. All these can cause inaccuracy. In addition, the distance between the ions at which the attraction and repulsion forces cancel each other may be significantly different from 1 nm and vary widely with different ion pairs, and the temperature of the plume is difficult to determine, and these two parameters affect the results of ion yield calculation exponentially. The intend of the calculation and more importantly the analysis is to give a rough picture of the ion extraction process in MALDI MS. Such a picture can be helpful for designing systems to improve MALDI MS sensitivity and resolution.