In this work, we present Raman spectra of both viable and neutralized C. parvum oocysts, captured by a Laser Tweezers Raman Spectroscopy instrument (LTRS). We also perform Raman mapping to illustrate the spatial distribution of individual components in the oocyst. Finally, we compare the Raman spectral signature with those of other common gastrointestinal pathogens to assess the possibilities to discriminate between pathogens.