Using the same workflow described above, the FTIR calibration was performed by the standard addition approach, prior to performing dynamic experiments. The auto-generated PLS model had a RMSECV of 43 mM, 42 mM, 18 mM, and 46 mM for 4, 5, TEA, and 6, respectively. This PLS model was applied to the dynamic run, then the process parameters and FTIR results were fed into the Julia software. The kinetic parameters were fitted as a trimolecular single step reaction, with fixed reaction orders of 1 for 4, 5 and TEA. The fitted pre-exponential factor (A) was 1.986 × 10 5 L 2 /mol 2 with an activation energy (Ea) of 49.5 kJ/mol. The activation energy in this case was higher than that of all previously-examined amidation reactions, meaning that the rate of the alkylation has a comparatively higher temperature-dependence.