Naturally, urea is hydrolyzed by urease enzyme (urea aminohydrolase E.C.3.5.1.5) that is frequently found in several organisms including bacteria, plant, algae, fungi, and invertebrates, but a higher amount occurs in soil microorganisms . Urease is a multi-subunit nickle containing metalloenzyme that is generally homo hexamers and each subunit contains an active site with two Ni 2+ ions . Urease has a vital role in nitrogen cycling in plants and improved the urea conversion rate about 10 14 times in soil . The emerging evidence revealed that the surface application of urea leads to its massive loss through several ways including leaching, immobilization, volatilization, and denitrification. However, after the surface application of urea into soil, urea is rapidly hydrolyzed within 24-48 h by naturally occurring urease into ammonium (NH4 + ), carbonate (CO3), and hydroxyl ions (OH-) . NH4 + further hydrolyzed into nitrite (NO2) by Nitrosomonas species and nitrite oxidized into nitrate (NO3) by soil living organism such as nitrobacter bacteria (see Eqs. :