Thermo Scientific coupled with an Orbitrap Fusion Lumos Mass Spectrometer (Thermo Scientific). The peptide mixture was resuspended in formic acid 0.1% and quantified by absorbance in nanodrop (Thermo Scientific). Then, 0.5 µg was loaded on a 15 cm length, 75 µm I.D. emitter, packed in-house with ReproSil-Pur C18-AQ 3 µm resin (Dr Maisch GmbH HPLC) for peptide separation. The column was equilibrated with 250 nL/min of 95% of mobile phase A (0.1% formic acid in water) and the mobile phase B (0.1% formic acid, 5% water in acetonitrile) was increased linearly up to 40% during 60-minutes gradient. The instrument was set to data-dependent acquisition mode with time between master scans set to 2 s. Only the potential type 2 peptides derived from Bm86-Ac-Cys1pP0 included in a list (Table ) were targeted for MS/MS analysis by using a normalized collision energy of 30 in collision induced dissociation (CID) and higherenergy collisional dissociation (HCD) fragmentation modes. Survey scans (300-2000 m/z) were acquired in the Orbitrap system with a resolution of 60,000 at m/z 200. The fragment ions were analyzed with a resolution of 15,000 at 200 m/z. The general mass spectrometric conditions were as follows: 2.30 kV spray voltage, capillary temperature of 175 ºC, predictive automatic gain control (AGC) set to 250% on MS1 and 220% for MS2.