We provide details on the free energy calculations in Figures S1-S16 and Tables S1-S5. GROMACS topology and coordinate files and parameter files (MDP files) are provided as a compressed archive file along with example scripts used in the analysis. We also provide additional data for completeness whenever we showed analysis only for a specific lambda window/replicate in the main body of this paper. More detailed explanations on the SI content can be found in README.md files.