As expected, the deformation of the vesicles is much more pronounced when the experiment is performed at 37°C, as can be seen from the highly irregular scattering curves in the top right plot of figure . It is also noteworthy that, despite its severity, the deformation occurs before the minimum time of 7.8 ms and no kinetics are discernible after this. Furthermore, repeating the experiment at both half and double the initial vesicle concentration showed that the deformation kinetics are not dependent on the vesicle concentration (Figure in the SI). When considered alongside the DLS results, which show that the hydrodynamic diameter of the vesicle remains unchanged, the results confirm that the observed deformation is not due to vesicle fusion or other interparticle interactions.