A modified analytical section was later employed to enable chip-based separations as a fast but low-consumption alternative to the commercially used C18-column (the general chipHPLC-setup was based on a recent joint publication ). For that purpose, the fluidic connections of the setup were modified according to Fig. , and the custom metal stage was connected directly to the MS source. The chip was mounted on an xyz-linear translation stage (T12XYZ, Thorlabs GmbH, Dachau, Germany), and the chip emitter was precisely positioned in front of the ESI-inlet. Furthermore, the flow rate of the binary eluent pump was lowered accordingly. Initially, the injection principle was evaluated and visualised by a sampling of fluorescent dyes. Then, the duty cycle of the injection principle was similarly automated as before (more details on the setup can be found in the ESI in section S2.2 and for the injection principle in S5.3).