Similarly, the loss of PE asymmetry has been observed during cytokinesis . Although the mechanisms behind this loss are not yet fully understood, they are crucial for effective cell division . During cytokinesis, the two membrane leaflets are coupled, with PE enriched in the outer leaflet of the cleavage furrow, alongside sphingomyelin and cholesterol. In contrast, phosphatidylinositol PI(4,5)P 2 is enriched in the inner leaflet. Disrupting the distribution of any of these lipids impairs cell division . The strong coupling of lipids, such as SM, cholesterol, and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P 2 ) between the two leaflets during cytokinesis shows intriguing similarities to the mechanism of FGF2 membrane translocation during unconventional secretion. These same lipids have been shown to play a critical role in FGF2 membrane binding and the formation of toroidal pores .