The optimal design, 𝒢 * , provides information on what transformations to perform but there is also additional Fisher information embedded in the design. It is this Fisher information that we used to perform many of the analyses presented here (Results). Indeed, 𝒢 * is the solution that a priori maximizes information about the ligands true binding free energies, 𝚫𝐆 𝐓𝐫𝐮𝐞 .