Methylamine hydrochloride (13.5 mg, 0.2 mmol) was dissolved in H 2 O (2.0 mL) and the pH of the solution was adjusted to 10.0. Sodium cyanide (9.8 mg, 0.2 mmol) was added followed by EA (1.2 μL, 0.02 mmol) and the pH of the mixture was readjusted to 10.0. Sodium nitroprusside dihydrate (59.6 mg, 0.2 mmol) was then added and the mixture was stirred vigorously at room temperature while bubbles of nitrogen gas evolved. After 24 hours, 450 μL of the mixture was removed for analysis by H-NMR spectroscopy. To this aliquot, 50 μL of 1.0 M NaH 2 PO 4 in D 2 O was added and the pH of the mixture was adjusted to 7.4 before the NMR spectrum was acquired.