Initially we focused our attention on the reduction of α-fluoroenones, which could be readily prepared via the Horner-Wadsworth-Emmons olefination of aldehydes using a fluorinated phosphonate under aqueous conditions. 21 Authentic samples of the racemic products were synthesized via the Pd-catalyzed decarboxylation of fluorinated β-ketoesters. Several EREDs, heterologously expressed in E. coli BL21 (DE3) were selected, including NCR from the bacterium Zymomonas mobilis which has been used successfully with a range of linear substrates, and our previously reported EREDs pQR1445 and pQR1907 from a drain metagenome which exhibited good organic solvent tolerance. The EREDs were co-expressed with glucose-6-phosphate dehydrogenase (G6PDH) to recycle the NADH co-factor in situ, utilizing D-glucose-6-phosphate sodium salt (D-G6PNa) as co-substrate. Enzyme lysates were employed in all reactions as these are typically used in industry, negating the need for costly enzyme purification.