With the evolved variant ACCOCHF in hand, we next examined the substrate scope of this biocatalytic enantioselective C(sp 3 )-H fluorination. Substituents at the 3, 4, and 5 positions of the aromatic ring were compatible, providing the corresponding fluorinated products in good to excellent enantioselectivity (2b, 2c, and 2d). Electron-withdrawing fluorine (2e) and chlorine (2f) substituents as well as electron-donating methoxy (2g) were compatible with this transformation. Tert-Amyl amides (2h) could also be transformed with good enantioselectivity. Furthermore, extended aliphatic substituents, including a propyl (2i), a homoallyl (2j) and a methoxyethyl (2k) group, could be efficiently fluorinated with excellent enantiocontrol. A tetrahydronaphthalene core (2l) also underwent biotransformation to furnish the corresponding enantioenriched fluorinated product. Additionally, tertiary C(sp 3 )-H bonds could also be efficiently and chemoselectively fluorinated to provide the desired fluorination product (2i) in excellent yields. A thiophene containing substrate 1n could also undergo C-H fluorination with excellent enantioselectivity and chemoselectivity (90:10 e.r., 2n:3n = 88:12, see the SI for details). Additional examples of unsuccessful substrates are provided in the SI. For example, primary C(sp 3 )-H bonds (1o) could not be efficiently fluorinated with evolved ACCOCHF. N-fluoroacrylamide 1p was transformed with low activity but excellent enantio-and chemoselectivity.