Follow General Procedure A, using 1.0 eq of alcohol 42, 1.5 eq. of NaH and 1.5 eq. of mesylate . . . . . . . . . . (keeping DMSO constant in all concentrations). 10uL of this dilution series was added to the plate after luminescence equilibration, and kinetic measurements of donor emission at 460 nm and acceptor emission at 618nm were collected every 3 min using a ClarioStar plate reader. milliBRET ratios were calculated as (Emission at 618nm/Emission at 460 nm) × 1000. Donor-contributed background or bleedthrough was corrected for by subtracting milliBRET ratios from no ligand control cells from treated cells. Corrected milliBRET ratios were plotted as a function of time in GraphPad Prism.