These adducts would then be detectable by denaturing polyacrylamide gel electrophoresis (PAGE) as an upward shift in mobility of the fluorescent staple 1. To minimize the ambiguity in identifying crosslinking products, we also created a minimal three-way construct (1HPjunction) (Fig ). 1HP-junction contains the same hairpin formed by staples 0 and 1 but uses a short "pseudo-scaffold" to mimic the scaffold in the 1HP origami. If the mechanisms of pso-TFO-driven crosslinking within the minimal 1HP junction and the full 1HP origami were the same, we would expect to observe identical patterns of Cy5-labeled adducts in PAGE analysis of both irradiated constructs.