The overlap matrices for the vanishing stages were very similar regardless of the restraint scheme, and the number of windows required for the vanishing stages could not have been reduced below approximately 30 in any case (see Figure as a representative example). In contrast, for both M-Hand-R and M-Rig-R, it appears that 10 windows would be sufficient, or even fewer in the case of M-Hand-R. For M-Hand-R. Convergence appears to be achieved after around 1 ns sampling per window (not including the 1 ns equilibration), which seems broadly similar to the bound vanish stage results (not including the 3 ns equilibration).