The practicality of conducting dSPE is of paramount importance for its implementation in the laboratory as a one-step high-throughput solution for lipid depletion. To ensure applicability in largescale studies, the preparation performance of the dSPE material was evaluated in a 96-well platebased format. The reproducible addition of loose C18 particle material from a single large-scale suspension was demonstrated by aliquoting the suspension using a standard 8-channel electronic pipette, resulting in an 7.7% RSD of sorbent weight measured across the 96 wells in plate.