Based on the many protein targets of diseases, multiple approaches including biological or in silico had been designed to screen new drugs towards the diseases treatment and these approaches are, very expensive and demand a great deal of background knowledge. where in pharmacology, many chemical libraries of synthetic small molecules, natural products or extracts were screened in vitro or in vivo , such as intact cells, whole organisms or cell-free systems to identify substances that have a desirable therapeutic effect and the recent experimental process to approach drug discovery includes several well defined biochemical examinations to screen those compounds that can interact or bind with certain binding partners, such as receptor/ ligand binding analysis(Hulme and Trevethick, 2010)enzyme-activity evaluation. For protein binding studies different technique can be used such as X-ray crystallography structural analysis NMR, , calorimetric, affinity chromatography, and protein mass spectrometry which are common strategic tools in drug discovery and these techniques aim to detect the separation of compounds from the studied proteins and monitor changes in intrinsic parameters of the targets upon forming a complex with tested drugs.