In Kembatta district, southern Ethiopia crushed roots are boiled with water and taken orally for the treatment of -Mitch‖, abdominal complains, and wounds . In Bale district, southern Ethiopia, fresh/dried root powder is decocted orally for the treatment of Diarrhea and sex problem in males. In Gubalafto district, northern Ethiopia, the roots are crushed and tie with butter for the treatment of broken bones . In Bati, eastern Ethiopia, the root is chopped, mixed with water and taken orally for the treatment of abdominal pain, weight loss and diarrhea . Therefore, form the above discussion; it is evident that many Ethiopian communities have been traditionally utilizing different part of R. cordifolia for the management of various ailments. However, we have not come across published reports on phytochemical investigation of the root bark part of R. cordifolia in the literatures. Therefore, here, we report the phytochemical tests antimicrobial guided isolation of compounds from the root bark of R. cordifolia.