The parasite Trypanosoma brucei brucei was cultured and propagated by infecting four healthy donor rats, ensuring an adequate supply of parasites for the experiment. Eighty experimental mice were divided into 20 groups, each consisting of four mice. Two concentrations of arthropod haemolymph (100% and 50%) were administered intraperitoneally in 0.3 µL doses using a 2 mL syringe with a 25-gauge needle. Haemolymph administration occurred four days before infection with T. b. brucei-infected blood (containing 2-3 parasites per microscopic field).