Finally, compound 4a (chlorhydrate salt) was evaluated using the in vivo bioluminescent mouse model . It was found to display no significant activity. As a positive control, a cohort of mice were treated in parralell with a curative regimen of benznidazole. (Figure ). The IC50 value represents the concentration that inhibits cell growth in 50%, the EC50 value represents the concentration that lyses 50% of the parasites, and CC50 value represents the cytotoxic concentration in 50% of mammaliam cells.