A fraction of frozen brain and liver samples from control (CNT) and compound KH1 (24) treated animals were-transferred into Precellys 2 mL ceramic bead tubes containing 1 mL SDS lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EGTA, 270 mM sucrose, 1% (v/v) Triton-X100) supplemented with complete EDTA-free protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor tablets (Roche) and placed immediately on ice. Then samples were placed in Precellys Evolution tissue homogeniser (Bertin Technologies) and tissues were lysed at 0 ºC for 3×30 seconds of cycles at 6800 rpm, with 30 seconds pause in between cycles. Samples were centrifugated at 4 ºC, 2000×g for 60 seconds to get rid of bubbles and supernatant transferred into new tubes. Lysates were cleared via centrifugation at 16000×g, 4ºC for 20 minutes. Protein concentration was determined by BCA assay (Thermo Scientific) and immunoblotting performed as previously described above. Immunoblots were quantified using ImageLab (BioRad) and band intensities were normalised to loading controls; GAPDH or Ponceau S staining and fold changes for protein of interest were plotted.