While MD simulations have shown that the energy barrier for inserting a single Daptomycin molecule into the membrane is relatively high , cooperative effects and supra-molecular complexes, involving both multiple Daptomycin molecules and calcium ions, can lower this energy barrier and facilitate membrane insertion. Daptomycin's ability to form oligomers or supra-molecular complexes is widely supported by experimental evidence and by MD simulation studies . The improved activity of the Daptomycin derivative Kynomycin , which exhibits enhanced oligomerization, suggests that favoring oligomer formation may be an effective strategy for increasing Daptomycin's antibacterial potency.