As a higher melting temperature has been observed for HheG D114C CLECs cross-linked with BMOE, we further investigated the temperature and pH profile of such CLECs. In line with the observed higher Tm, HheG D114C CLECs displayed their highest relative activity at 40°C in the azidolysis of 1, while highest relative activity of soluble enzyme was obtained at 25°C (Figure ). Moreover, also the determined T50 value of HheG D114C CLECs was 12 K higher compared to soluble enzyme (Figure ), further confirming an increased thermal stability upon CLEC formation.