The same pattern is observed using the angle of the highest density from Kernel Density Estimation (KDE) of the data (Table ). Figure shows the distribution of angles and confirms the trend towards populations with a higher angle on phosphorylation of wild-type and the opposite trend for G159D. The distribution of angles, as quantified by the Full Width at Half Maximum (FWHM) values, is also phosphorylation-dependent (Table ). Unphosphorylated WT has a very broad distribution of accessible angles, which upon phosphorylation is reduced by ~25% whilst in contrast G159D, in both states, has a much reduced range which is ~25% less than phosphorylated WT; there is also an opposite effect observed upon phosphorylation with an increase of ~7%.