The most time-consuming and labour-intensive aspect of this assay was the pipetting, prompting us to optimise this further. Our fully automated method allowed us to collect 24 Hill plots in a single day. Each 384-well plate required approximately two hours for preparation in a fully automated process that does not require researcher intervention after the method started, followed by 30 minutes for assessment on the plate reader to generate 12 Hill plots. Future improvements may be achievable by incorporating a liquid-handling robot equipped with multi-channel pipettes. This would facilitate the batch-wise addition of pre-prepared DMSO solutions to the vesicle-containing plates. This would not only expedite the addition of transporter solutions but also increase the number of plates prepared daily while minimising lipid degradation, given that the vesicles should be used within 2-3 hours. However, we did not explore this on the Opentrons OT-2 due to concerns about instrumental error for low-volume transfers, as the reported accuracy of the 8-channel GEN2 pipettes at 1 µL is ±20%. Maintaining the DMSO concentration at a precise 1% v/v is crucial, as any variation could interfere with results due to DMSO's quenching properties.