The 2D structure of 6-gingerol (CID 442793) was obtained from the PubChem database() in SDF format. To ensure its molecular geometry was accurate for further analysis, the structure was optimized using Chem3D. This step was crucial to reduce steric clashes, refine bond angles, and achieve a stable, energetically favorable conformation, enhancing the reliability of the computational predictions. After optimization, 3D SDF format of the structure was converted to PDB format using Open Babel, a well-known software tool commonly used in computational chemistry for converting and preparing molecular structures.