The introduction of pectin, a biotic elicitor, further enhanced paclitaxel production and growth. Pectin, a polysaccharide found in plant cell walls, stimulated a dramatic increase in paclitaxel yield. The total taxane content increased by up to 116 3. **Mathematical Modeling**: A Gaussian function was applied to model the relationships between independent variables (pH, sucrose, and ammonium phosphate concentrations) and dependent variables (growth rate and paclitaxel yield). This enabled precise prediction of the optimal conditions for maximizing paclitaxel production. For instance, the model indicated that the highest growth rate and paclitaxel yield occur at a sucrose concentration of 4.6 4. **Enhanced Taxane Profiling**: Using HPLC and LC-MS, the study confirmed the presence of various taxanes, including paclitaxel, baccatin III, 10-deacetyl paclitaxel, and cephalomannine, produced by *A. alternata* under the optimized conditions. Notably, pectin enhanced the production of specific taxanes, particularly 10-deacetyl baccatin III and paclitaxel, revealing a targeted enhancement of the taxane biosynthesis pathway through pectin elicitation.