Considering the relevant bioactivities of related decalin structures, these enzymes have the potential to be used for the biocombinatorial decoration of related scaffolds to create novel derivatives. Additionally, carboxyl methyltransferases such as BueF are of biotechnological interest. Heterologous expression of bue genes in the chassis A. nidulans proved a more favourable strategy for the production and isolation of 1 than in the native host, obtaining a ~45-fold increase in the yield of 1 per kg of substrate compared to A. burnettii. Interestingly, we also observed that the strains of A. nidulans harbouring AMA1-based episomal vectors, which require auxotrophy selection for maintenance, can sustain production in grain substrates such as jasmine rice with yields as high as 468 mg/kg in the case of 2. Additionally, we showed that TF overexpression can be effective to upregulate AMA1encoded heterologous BGCs in A. nidulans, a strategy also used by Hu et al. Overall, this work highlights the benefits of heterologous expression in an engineered A. nidulans chassis even for BGCs that are active on their native host. Finally, this work contributed to the understanding of the widely spread burnettiene and fusarielin BGC family. As uncharacterised clusters in other Aspergilli, for example A. fumigatus cluster 4, 2,27 present a higher identity and synteny with bue gene cluster than to the FSL gene cluster, they are likely to encode compounds structurally more similar to burnettienes than fusarielins (Fig. ). Thus, the results from this work can help interpret this extensive and elusive cluster family in those fungi.