In addition, Belsare and Coté reported a novel paper-based colorimetric assays for monitoring gestational diabetes at the point-of-care. This system utilizes an aptamer-based assay with gold nanoparticles to produce colorimetric measurements on a paper fluidic test. The sandwich aptamer format was used to construct the assays, and the colorimetric intensity was measured by scanning the capture layer of the paper fluidic device using a standard flatbed scanner followed by RGB analysis. The system is able to determine the concentration of glycated and serum albumin in their physiologically relevant ranges of 50-300μM and 500-750μM, respectively. The placement of gold nanoparticle probes in the paper fluidic system enables automation and minimizes user intervention. The use of aptamers as recognition elements and colorimetric transduction with gold nanoparticles ensures that the system has the required sensitivity, selectivity, and dynamic range for physiologic monitoring. In summary, this work has developed two aptamer-based colorimetric assays for measuring the percentage of glycated albumin, which have been immobilized on a paper fluidic platform for sensitive and low-cost point-of-care use. The system can measure glycated albumin and serum albumin in the physiologically relevant ranges of 50 to 300μM (GA) and 500-750μM (serum albumin), respectively.