Aspergiolide B (12) was isolated from A. glaucus as a new analogue for aspergiolide A (11) . Aspergiolides A-B (11-12) exhibited potent cytotoxic activities against both adenocarcinoma human alveolar basal epithelial cell line A-549 with IC50 values of 0.13 and 0.24 μM and human leukemia cell line HL-60 with IC50 values of 0.28 and 0.51 μM, respectively indicating that methylation of one hydroxyl group in aspergiolide A to be a methoxy group in aspergiolide B slightly affected the cytotoxicity of aspergiolide A.