INTRODUCTION: A carcinogen is any chemical, physical or biological compound that has the potential to induce cancer in living tissues . Exposure to carcinogens can be through inhalation, ingestion, or absorption into the bodies of multicellular organisms . Alkylating agents are members of a structurally diverse group of DNA damaging compounds which form adducts at ring Nitrogen and extra-cyclic Oxygen atoms of DNA bases N-Nitroso-N-ethyl urea (NEU), an ethylating agent has been described as a very potent transplacental teratogen and carcinogen in rodents Human carcinogens could be genotoxic, which means to directly alter the genetic material of target cells . Other carcinogens are classified as non-genotoxic, in which case are capable of producing cancer by some secondary mechanism and not by direct gene damage . These secondary mechanisms include the abilities of an agent to alter DNA repair or cause genomic instability, epigenetic alterations, oxidative stress, chronic inflammation, immunosuppression, immortalization or alter cell proliferation, cell death, or nutrient supply . The effect of carcinogen exposure has also been established to alter biochemical and hematological profiles in humans Secondary metabolites from plant sources like flavonoids, alkaloids, saponins, and others have been found to be potent anticancer agents . The anticancer effect of these natural products is mediated by different mechanisms, including apoptosis induction, immune system modulation, and angiogenesis inhibition . Senna alata is highly significant due to the abundant bioactive chemical compounds which have been reported to display numerous biological activities These biological activities include antimicrobial, antioxidant, anti-cancerous, anti-mutagenic and anti-diabetic activities. Leaves of S.alata plant have been used in treatment of eczema, ringworm, asthma, bronchitis and in poisonous insect bites .