shows the set-up for the various sample lengths of 2.4, 1.8, and 1.3 cm respectively corresponding to sample volumes of approximately 0.33, 0.25, and 0.18 ml in a regular Norell NMR tube (0.42 and 0.50 inner and outer diameter). These reduced-volume samples are called SEN (Solvent Encoded) samples henceforth. The standard sample of 4 cm length (0.55 ml) is also shown. While in each of these samples, the total length of the sample was 4 cm, it is the middle layer lengths that were adjusted to 2.4, 1.8, and 1.3 cm respectively by adjusting the top and bottom layers of immiscible solvents accordingly. In each sample, the middle layer containing the analyte is chosen to be symmetric about the center of the total length-4 cm as displayed. These reduced sample volumes were evaluated for their signal-to-noise ratio (S:N) and other aspects of spectral quality with respect to the standard volume sample of 0.55 ml that fills a 4 cm sample length. It is to be noted that solvent encoding using 2.4, 1.8, and 1.3 cm sample lengths lead to 1.66, 2.22, and 3.07 -fold reduction in deuterated solvent quantity respectively relative to that of the standard 4 cm sample length.