Nanodiamonds are excellent candidates as nanofillers for biomedical applications, in light of their excellent biocompatibility, mechanical properties and tunable surface chemistry. They also have potential for quantum biosensing, as fluorescent nanocarriers for the intracellular delivery of biomolecular cargo, , as nanotherapeutics, and they were embedded in contact lenses for the enzyme-triggered sustained delivery of bioactives. However, their low colloidal stability in biological and/or ionic environment has limited their use in hydrogels, despite their remarkable properties. Considering that self-assembling peptides are amphiphilic in nature and demonstrated to significantly improve the dispersibility of CNOs in aqueous buffers, we thus sought to investigate whether they could exert a positive effect also on the dispersibility of nanodiamonds to attain their embedding into nanocomposite hydrogels formed in physiologically compatible conditions.