We also obtained a slope of 0.91 ± 0.04 in a log-log plot of the current vs. scan rate (Figure ). Theory predicts that a slope of 1 is indicative of an entirely adsorption-driven process while a slope of 0.5 indicates a solely diffusion-driven process . Our slope indicates that the signal we obtained is primarily adsorption-driven with some contribution from diffusion and this is in agreement with those reported by others . As summarized in Figure and 6b, the original MSW did yield significantly lower current density than the dopamine waveform at all concentrations (****p < 0.0001). The optimized MSW, however, yielded significantly higher sensitivity for Trp detection than the original MSW at all four concentrations that were evaluated (****p<0.0001). The optimized MSW yielded higher current density for Trp than the dopamine waveform at all concentrations evaluated (****p<0.0001). These conclusions were further validated by the calculated limit of detection (LOD) and quantitation (LOQ) (Table ). LOD and LOQ were calculated from standard deviation of the baseline noise in the IT curves and the corresponding calibration curve for each waveform (n = 5 for each category). There is no statistical difference between those reported for the dopamine and original MSW (p = 0.0588). Measurements using the optimized MSW, however, did show significantly lower LOD and LOQ than those for the dopamine (p < 0.01) and original MSW (p < 0.05). We conclude, therefore, that the optimized MSW provided conditions for the most sensitive detection of Trp of the three waveforms. In fact, based on these data, we can report a 4-fold improvement in the limit of detection using the optimized waveform compared to the dopamine waveform and a 20-fold better sensitivity compared to the original MSW.