Nucleic acid (NA) nanotechnology is a bottom-up nanofabrication approach that harnesses the self-assembly properties and well-understood structure of nucleic acids to create molecular objects or patterns ranging from a few nanometres to a few microns in characteristic size. DNA origami is a subfield of NA nanotechnology in which a long single-stranded DNA "scaffold" strand is woven in two or three dimensions using a few hundred shorter DNA "staple" strands. DNA origami are straightforward to design, relatively cost-effective to produce, and offer potential applications in a wide range of disciplines. Examples of applications of DNA origami in biomedicine include vaccines, enzymatic cascades, biological nanosensors, nanorobotics, drug delivery, super-resolution microscopy, structural biology, basic singlemolecule research, and many others.