The perfusion technique involved injecting the gelatin solution into the vascular scaffold while simultaneously withdrawing it. This dynamic process allowed the gelatin to flow through the tubular structure, resulting in a thin and uniform layer adhering to the scaffold's lumen. To optimize the coating process, each injection of the gelatin solution lasted for 10 seconds. The process was then repeated three, five, and ten times, respectively, to evaluate the effect of multiple injections on the thickness and attachment of the gelatin layer to the scaffold's lumen. By systematically varying the number of injections, the ideal conditions for achieving the desired gelatin coating thickness and uniformity were determined. The hierarchical structure was assessed through SEM to examine layer morphology and attachments.