We also tested the phase separation of ternary systems composed of DPPC, an unsaturated PC lipid (DLiPC, DOPC) and cholesterol at 42:28:30 mol% ratios and at both 300 and 290 K (Figure ). The DPPC:DLiPC:CHOL system phase separated, inducing the formation of a more ordered DPPCrich phase containing the large majority of cholesterol incorporated within it, and a more fluid DLiPC phase (Figure ). This phase separation occurs at both 290 and 300 K, and is in good agreement with both Martini 2 and experimental results .