importance of acknowledging the unique chemical characteristics of each polyelectrolyte (PE) chain when functionalizing a nanochannel, rather than simply categorizing them as anionic or cationic chains. This forms the foundation that emphasizes the need for a more nuanced atomisticlevel understanding (beyond the coarse-graining based simplifications) of the PE chain behavior for optimizing the nanochannel performance. Simulation procedure for observing electrophoresis of ungrafted PMETAC chain in nanochannel;