Following identification of ligands for both SmBRD3 (1) of miracidia to transform into sporocysts; this was likely due to high expression of smbrd3 in these lifecycle states (Figure ). At concentrations as low as 2.00 μM, the majority of the miracidia were prevented from transforming into sporocysts. It is notable that most SmBRD3(1) ligands did not show effects against schistosomula, miracidia, or adult worms. Given that both compound 22 and OXFBD03 (2) can be delivered to worms, these results suggest that SmBRD3(1) and SmBRD3 (2) have different functions, and consequently their selective inhibition results in different phenotypes.