Fluorescence images were acquired on an upright confocal microscope (Nikon A1R HD25, Nikon Canada, Mississauga, ON, Canada) using a 10X/0.45NA objective for quantification images and a 25X/1.1NA objective (water immersion) for high-resolution images. The channels were optimized independently based on the most intense samples to avoid pixel saturation. All the images from experimental and control samples were acquired using the same conditions on the microscope. Z-stack images of the center area of the dog-bone shape constructs were taken and the mean intensity per pixel of the cells was obtained using Image J software