S. haematobium eggs were obtained from the gut tissue of infected hamsters in accordance with the project license that the Dutch Central Authority approved for Scientific Procedures on Animals (CCD) (animal license number AVD116002017106). Syrian gold hamsters (RjHan:AURA) were obtained from Janvier Labs. Infection was performed percutaneously by placing the animals in a bucket containing a thin layer of water infested with ~1200 S. haematobium cercariae. After 45 min, the animals were taken out and allowed to dry inside their cages. At 16-20 weeks post infection, the hamsters were sacrificed by IP injection of an overdose of Euthasol (pentobarbitalnatrium) after sedating them first using isoflurane. Eggs were obtained by collagenase B digestion of the tissue, followed by extensive washing, after which the eggs were stored in a 4% formalin solution at 4°C for approximately eight years before being used. The morphology and size of these gut-derived eggs represent those normally seen in the urine of infected humans.