An in-house algae strain (Schizochytrium sp. MASA#4) was grown in a 6 L bioreactor (Infors HT, Invitrogen Technologies) to produce the biomass required for this study. The inoculum was prepared in medium containing glycerol (10 g/L), yeast extract (1 g/L), peptone (1 g/L) and incubated for 48 h at 25 °C with agitation at 150 rpm. Inoculum (10%) was used in the production medium containing glycerol 120 (g/L), yeast extract (10 g/L), peptone (1 g/L), MgSO4 (10 g/L), and sodium acetate (4 g/L) in a bioreactor and cultivated at 25 °C for 5 days. The pH of the medium was maintained at 6.5 using potassium hydroxide solution. The culture medium was harvested after 5 days and subjected to freeze drying to obtain dried biomass.