Recently, researchers have demonstrated controlled transport of multiple fluid flows for sequential delivery of fluids in two-dimensional paper networks as a means to extend the capabilities and performance of lateral flow technologies while minimizing manufacturing costs. These advancements in paper-based diagnostics hold great potential for point-of-care testing and reducing the cost and complexity of diagnostic tests. The transition from traditional microfluidic systems to a paper-based system would have numerous advantages, including reduced costs, simplified fabrication, elimination of pumps and power requirements, and the removal of issues caused by bubbles that often occur in microfluidic systems. In recent research, several conventional microfluidic devices that utilize adjacent flow streams were translated onto 2D platforms, demonstrating the feasibility of paperbased systems to perform hydrodynamic focusing, analyte extraction from a complex mixture using diffusion, micromixing, and rapid dilutions. These Microfluidics has emerged as a rapidly growing field in recent years, offering numerous advantages over traditional methods for various applications such as biomedical analysis, environmental monitoring, and chemical synthesis. Among the many areas of microfluidics research, the manipulation of fluids plays a crucial role.