The analysis of enzymatically released oligosaccharides was carried out on a LCMS-2020 system (Shimadzu, Japan) equipped with an ACQUITY UPLC BEH amide column (2.1 mm x 150 mm, particle size 1.7 µm, Waters, USA). 20/80 (v/v) acetonitrile/water (A) and 80/20 (v/v) acetonitrile/water (B) were used as eluents. Both eluents contained 10 mM ammonium formate and 0.2 % formic acid and the following gradient program was applied: 5 min at 100 % B, in 26 min linear to 20 % B, in 1 min back to 100 % B and finally 8 min at 100 % B. The analysis was carried out at a flow rate of 0.4 mL/min and a column temperature of 35 °C was used. MS detection with electrospray ionization was carried out in negative full scan mode (m/z 250-1000). For sample preparation, 50 µL of the hydrolysate were mixed with 150 µL acetonitrile to precipitate undigested high molecular weight material and to adjust the solvent composition. The samples were centrifuged for 4 min at 16.100 x g and the supernatant was used for HPLC-MS analysis.