Despite this challenge, a potential avenue for future investigations lies in the adoption of a Pure-Sal saliva collection kit. This approach could mitigate the interference caused by mucins and other substantial proteins that might compromise the integrity of the FID assay signal . The study by Dalirirad et al. underscore that the utilisation of the Pure-Sal kit, coupled with Ultraviolet (UV) absorbance measurements, led to a significant reduction in protein content. This kit presents a promising prospect for enhancing the success of the TO-aptamer based FID assay .