To benchmark the modified parametrization approach, the following analyses were conducted. First, the vibrational power spectra for the fragments were calculated with the GROMACS tool gmx velacc. These spectra were calculated using both parameterization approaches: the previous one described above , and the current modified approach with restricted force constants. Moreover, the influence of the reparametrization on the agreement between the atomistic and coarse-grained distributions was assessed using a mismatch score derived from the Swarm-CG tool. Finally, the optimal number of conformers needed to achieve sufficient convergence of equilibrium distances and angles was determined.