For each fragment, 2 wells of an MRC 3 well crystallisation plate -PS (Jena Bioscience, DE) were prepared with a nominal amount of 100 mM fragment in 0.4 µL and dried at 40 °C for 18 hours. 0.4 µL soaking solution (2.05 M sodium malonate, 9.5 % (v/v) glycerol, 5 % (v/v) DMSO, pH 6.5) was added and incubated at RT for 5 minutes. Crystals were soaked by transferring at least three crystals into each drop and incubating for 3 hours at 20 °C. Two crystals per condition were picked and flash-frozen in liquid nitrogen. An additional number of 24 crystals were soaked in the soaking solution without presence of a fragment for data collection of the apo structures for subsequent analysis using PanDDA.