The solubility of compounds was estimated by DLS that directly measures compound aggregates and laser power in solution. Compounds were serially diluted directly from DMSO stocks, then diluted 50x into filtered 10 mM Hepes pH7.4, 150 mM NaCl, 5 mM MgCl2, 0.03% Tween20 (2% DMSO final). The resulting samples were then distributed into 384-well plates (black with a clear bottom, Corning 3540), with 20 μL in each well. The sample plate was centrifuged at 3500 rpm for 5 min before loading into DynaPro DLS Plate Reader III (Wyatt Technology).