It is worth noting that, due to the absence of heteronuclear spin-spin coupling, water does not give rise to ZULF NMR peaks at a truly zero magnetic field. This can be seen as an advantage of zero-field NMR compared to high-field 1 H NMR, where water protons give rise to a very intense and broad signal that can overlap with other signals . The feature makes zero-field NMR a promising modality for the analysis of biological samples with a high concentration of water (e.g., blood, urine, or cell cultures) as there is no need for solvent suppression.