The parameter se}ngs used for the NPD analysis based on SIEVE algorithm, were set as follows: a m/z range of 200-2,000, a RT range of 5.0-70.0 min, a frame Nme width of 0.50 min, a number of maximum frames of 5,000 and a peak intensity threshold of 1.0% TIC. Several filter se}ngs were applied aper data processing with the aim of reducing false negaNves/posiNves, including pacern recogniNon (PR) element = 0 (only features with monoisotopic mass will be included); PR size > 1, which means at least one addiNonal isotope must be detected; charge state range between 2 and 5, and MS peak area raNo (allows for an x-fold change, either more or less, in terms of average intensity) not between 0.1 and 10. The four commercial DP batches were analysed using the DS as the reference material.