Interestingly, glycerol monodecanoate became negatively related to PC1 in AL 4, which is consistent with Figure (a) and Figure where those samples that have a larger percentage of glycerol monodecanoate did not to form vesicles. This is in contrast to previous studies that used glycerol monodecanoate to stabilize membranes when mixed with decanoic acid/decanoate in compositional amounts from 10 to 50%. Glycerol monodecanoate is the glycerol ester of decanoic acid, and thus has been suggested as an "evolutionary step" in membrane composition between the simplest amphiphiles and phosphoglycerolipids. However, our results show that an abundance of this molecule, at about 10-20% of the total amphiphile concentration, reduced the probability of finding vesicles. This result highlights the complexities of predicting vesicle-forming compositions for the origins of life and suggests that glycerol monodecanoate may be less prebiotically relevant than previously thought.