Interestingly, there is a clear recall ceiling for IsoDec, likely because the charge for these clusters is never correctly assigned (see Figure ), no matter how loose the filtering parameters are. In the case of the cluster in Figure , the distribution to the left is selected first, and multiple shared isotope peaks are knocked down. The knockdown of these shared isotope peaks results in incorrect charge assignment for the distribution highlighted in Figure . Similar recall ceilings were previously observed for THRASH-based algorithms. To achieve higher recall, new strategies to handle cases where the charge is incorrectly assigned are required. Additional details on limitations and future directions are provided below.