The use of graphene-based nanomaterials as electrodes for glucose sensing has shown promising due to their high conductivity and charge mobility. However, challenges remain in addressing the electron transfer between the current collector and the catalytic active sites, which can be improved through the use of conductive materials that lower interfacial charge resistance and overpotential. Despite this, graphene-based electrochemical glucose sensors offer high stability, little residual current, high capacitance, vast flexibility, and substantial robustness, making them a promising option for glucose sensing applications. Further research is needed to address the remaining challenges in utilizing graphene materials for glucose sensing, including further enhancement on the conductivity, stability, real-sample matrix effects, and their integrations with smart and wearable device.