Also shown is the structure of the amino acids in the binding site and their polar interactions with surrounding residues. The critical interactions between the binding site and the ligand in this pocket combined with the pocket's remodeling upon binding imply that inhibitors here would significantly impact InvC function . A shift from generation 0 to generation 3 is also present, though less obvious. Proportionally, there is a higher amount of ligands with binding affinities from -4.5 to -6.5 in generation 3 compared to generation 0. The peak in the generation 3 graph occurs at -5.5 to -6.0 kcal/mol, while the peak in the generation 0 graph occurs at -4.5 to -5.0 kcal/mol. From generation 3 to generation 7, the spread of the graph decreases, and the graph shows an increased proportion of compounds with binding affinities from -6.0 to -7.0. The peak of the graph remains at the same binding affinity. From generation 7 to generation 10, the proportion of compounds with binding affinities better than -5.0kcal/mol appears to decrease slightly, indicative of the previously discussed stagnation in later generations. The mean binding affinity of each generation was compared to the mean binding affinity of generation 0. We hypothesized that the mean binding affinity of generations 1-10 would be significantly smaller than the mean binding affinity of generation 0, setting a standard alpha-value of 0.05. The results of the significance test are shown below. mutations produced by AutoGrow4. The structure, highest binding affinity, and the first six digits of the compound code are provided in Figure .