a -5 μL is the minimal working volume for sample injection, < 250 nl of sample is used for the analysis; bbased on the method used here which includes washing steps; c -asymmetric peak = not diffusive or slowly diffusive aggregates; spikes = large, non-diffusive aggregates; absence of spikes and asymmetric peak = large and macroscopic aggregates that do not enter the capillary; d -removal of most aggregates by centrifugation followed by FIDA allows more reliable sizing of the residual soluble species; e -using Type 42.2 Ti Fixed-Angle Titanium Rotor (Beckman); f -e.g. pipetting in and out of the centrifugation tubes and absorbance measurement off-line; g -using 384-well plates and 30 min read time/plate; h -ThT signal is not always linearly dependent on fibril concentration; i -using DSF capillary-based platforms such as Prometheus Panta (NanoTemper).