In order to dock the aptamer and cortisol different folding and docking models were utilised and computed in python. Firstly, the secondary structure was computed which produced the dot-bracket notation of the aptamer. The minimum free energy of ∆G = -6.6 kcal mol×K was determined computationally based on this secondary structure . Secondly, the aptamer was folded as a three-dimensional structure based on the secondary structure . Thirdly, the aptamer and cortisol were docked using molecular simulations with Haddock . Final rendering was performed in PyMOL and calculations and data analysis were conducted with python.