Second, the MITE repository could act as a collection of tailoring enzymes/maturases for biosynthetic engineering and optimization of BGC pathways. By searching for specific reactions or substrate specificities, researchers would have access to an evidence-based parts catalog that could be used for rational biochemical engineering. This information could be used in mix and match approaches for the creation of new-to-nature molecules, and even for de novo design of new pathways. Third, the reactions encoded in the MITE repository could be used for in silico biosynthesis of NPs. MITE reaction SMARTS can be applied in parallel or consecutively, and associated reaction metadata allow to fine-tune in silico biosynthesis. Therefore, if all tailoring enzymes/maturases of a BGC are characterized, this allows to reconstruct the full biosynthesis of a NP, resulting in a single or multiple final structure(s). However, even for BGCs with only singular tailoring enzymes characterized as MITE entries, an approximation of the putative product structure can still be reached. Such a structure could be then used in further analysis, such as prediction of MS/MS spectra for matching against metabolomics data, for search in structural databases, for the prediction of biological function or activity, or for metabolic modeling.