Original dataset. We initially gathered 348 peptide sequences from DBAASP (Database of Antimicrobial Activity and Structure of Peptide, ) using the Ranking Search option and the following selection criteria: the sequences could be of any sequence length, of natural or synthetic origin, and labelled as monomers. Their chemical structure must contain only proteinogenic residues (no unusual amino acids and interchain bonds) without any modification in the N-terminus. We kept the sequences that could be amidated on their C-terminus. All available human cancer cell lines were specified in the database, regardless of the culture medium. The activity measurements were sought after to be reported as IC50, defined as the peptide concentration at which cell viability is reduced by 50% and where a low value denotes greater effectiveness . All peptides demonstrated anticancer activity against at least three of 77 listed cancer cell lines. Only the peptides that targeted the lipid bilayer(s) were chosen since they would represent membranolytic peptides. Finally, we also selected the sequences that were evaluated against more than two cancer cell lines leading to a final set of 138 peptide sequences.