Given the broad pharmacological properties of these TA scaffolds and failure to detect REV-ERB protein enrichment, the gene expression pattern of protein hits from ECAL targeted labeling method across various cell types was studied using a single-cell RNA sequencing (scRNAseq) BioTuring Database comprising 255 gene studies. Through the analysis of ranked expression, we observed that majority of these genes are most prominently expressed within lymphoid, T and B cell populations (Figure ). In addition, a subset of 19 genes were detected that shared similar expression patterns across cell subsets to RORγ and REV-ERBα (NR1D1) suggesting shared function or biology (Figure ). The reported role of SR9009 in modulating Th17 cell differentiation 41 led us to next examine the gene expression profile of enriched protein hits within naïve CD4+ T cells and differentiated Th17 cells, as annotated in the BioTuring resource. From this analysis we identified genes that are abundantly and commonly expressed in cells representing Th17 and naïve CD4 T cells (>70%), including FAU, YBX1, SUB1 and APRT (Figure ). We also observed some genes that are more abundantly found in Th17 cells as compared to naïve CD4 T cells (e.g. TPI1, PPIB, LYAR, ELOVL5 and GYG1). Collectively, these results suggest that the Th17 differentiation effects of these compounds potentially occur in a REV-ERB binding independent manner and further highlight their possible pleiotropic nature.