One example is the work performed by Gupta et al. . They used the known ligand-based pharmacophore modelling approach to find novel human TLR7 modulators based on the set of TLR7 agonists with confirmed experimental activity. They created a pharmacophore model and screened the natural hit compounds from the InterBioScreen Natural product database. They filtered the screened compounds and based on molecular docking and interaction analyses, they selected the most interesting compound -STOCK1N-65837 (an indoline derivative natural alkaloid). The compound was further validated with MD simulation. Authors underlined that further experimental validation is necessary to confirm the activity of the compound, however, their results already provided a basis for further designing of natural modulators targeting TLRs.