The following names were used to facilitate the interpretation of the poly(Nisopropylacrylamide) (PNIPAM) and nanoparticles' conformation: 1) extended/opendescribing hydrated short, not bent chain; below LCST; 2) closedbent short polymer chains or short chains connected via hydrogen bonds at the nanoparticle surface; above LCST or in the nonaqueous environment. HT (high or higher temperature refers to the temperature of 40 °C -42 °C, and LT (low or lower temperature) refers to 18 °C -20 °C of the subphase. The definitions of "open" and "closed" conformations are grounded in the behavior of the PNIPAM capping layer, which undergoes temperature-dependent changes. Below LCST, PNIPAM chains adopt an extended "open" conformation due to their hydration and hydrogen bonding with water. Above the LCST, PNIPAM transitions into a compact, "closed" globule-like state as these hydrogen bonds break, causing the chains to collapse. These conformational changes directly impacted the assembly of nanoparticles at interfaces and were critical in designing the PSiFe system.