GLY146(A)-PHE109(B) and LEU32(A)-LEU32(B). OLIVES correctly identifies these backbonebackbone hydrogen bond partners and places them in the quaternary network. Additionally, an essential hydrogen bond is located in the middle of the transmembrane region between GLN94(A)-GLN94(B), which is also correctly identified by OLIVES. The native contacts of the OMPLA monomers are reproduced using both OLIVES and GoMARTINI (Figure ,C) and we do not observe any distortion of the β-barrel, however, a more in-depth study of β-barrels of various sizes is needed to rule out the distortion effects reported by Desikan et al. Interestingly, the interface native contacts (Figure ,D) are reproduced very well without a quaternary network using either OLIVES and GoMARTINI,