The EDX analysis confirmed the presence of CuO NPs, giving a ratio of 34.42% oxygen and 65.58% copper, corroborating that the main components of the biosynthesized sample are copper and oxygen. The absence of additional elements in the EDX spectrum suggests a high purity of the nanoparticles. Overall, the EDX results validated the chemical composition of the CuO NPs and the effectiveness of the synthesis method employed .