Thiamine phosphate metabolism also differ between VI and LL muscles during postmortem aging of pork . Thiamine triphosphate (ThTP), an abundant thiamine derivative specifically in porcine muscle, is converted to thiamine through thiamine diphosphate (ThDP) accumulation 24 h postmortem, with a difference between the two muscle types. In addition to the coenzymatic role of ThDP, thiamine and its derivatives are also known to participate in biological responses to oxidative stress, both as ROS scavengers and regulators of gene expression. Furthermore, ThTP and adenylate ThTP activate bovine liver glutamate dehydrogenase, which generates 2-oxaloacetate from glutamate to provide citrate cycle substrates . However, the roles of thiamine and its derivatives in postmortem muscle metabolism require further investigation. Intermuscular differences in postmortem metabolite distributions have also been observed in bovine studies focusing on early postmortem energy metabolism and lipid oxidation . In addition, the content of glycerophospholipid, an essential compound of the mitochondrial membrane, differed between the longissimus dorsi (LD) and psoas major (PM) muscles in early postmortem . This could be explained by varying contents of mitochondria between the two muscle types accelerated the oxidation of glycerophospholipids, and thereby, damaged the mitochondria. Since mitochondria play a crucial role in energy generation through β-oxidation and the citrate cycle, in connection with glycolysis and AA metabolism, all metabolisms related to energy substrates and sources likely differ between slow and fast muscle types.