First, the 1 H NMR analysis was performed to verify the chemical structure of synthesized polymers and calculate the real weight percentage of respective building blocks in comparison to theoretical calculations (based on monomers feed). The changes in chemical structure after degradation were also characterized using 1 H NMR and all results are shown in Figure . Additionally, detailed information on the formulas and calculations derived from the 1 H NMR data, including the real weight percentage of respective building blocks and the changes in chemical structure after degradation, can be found in the Supplementary Information (Table ). The composition determined from 1 H NMR (Table ) indicated a final wt% of hard and soft segments differing from the initial feed ratio revealing that some losses of monomers occurred during the stepwise decrease of the pressure during the reaction. The analysis of intensity and integrals of the signals allowed also the calculation of molecular weight (Mn) of copolymers along with the segmental composition before and after the degradation (Table ). The weight percentage composition calculated for PBS-DLS of theoretical composition 70-30 wt.% significantly decreased to 63-37 wt.% after the degradation while for samples with theoretical 60-40 wt.% PBS-DLS segments, the change was almost negligible, i.e., 61-39 wt.%. This is an indication that shorter PBS sequences are much easily removed at their higher (70 wt.%) content, while increasing content of plant-derived dilinoleic diol hinders an easy penetration of degradation medium to remove butylene succinate segments. Comparison of segmental composition of copolymers containing PEG indicated that hydrolytic degradation assisted by the enzyme lead to decrease of PEG content by a half (from 7 to 3 wt% for the copolymers with 70% hard segment and from 10 to 4 wt% for the copolymers with 60% hard segment). The analysis of macromolecular structures from 1 H NMR revealed significant differences in Mn, as presented in Table . The Mn of PBS-DLS copolymers (as calculated by NMR) decreased in both segmental copolymers, e.g. from 38.6 kDa to 31.3 kDa (for 70-30 wt.% hard-soft segments) and from 31.1 kDa to 29.2 kDa (for 60-40 wt.% hard-soft segments). The slight differences in molecular weight changes is characteristic for surface erosion, which typically occur in polyesters, like PLA or PCL . Notably, the addition of PEG resulted in much higher decrease in molecular weight of the PBS-DLS-PEG copolymers, with a decrease from 27.9 kDa to 19.1 kDa (for 70-25-5 wt.% PBS-DLS-PEG segments) and from 28 kDa to 17 kDa (for 60-35-5 wt.% PBS-DLS-PEG segments) what is an indication for acceleration of the degradation process in the presence of hydrophilic building block. It is important to note that enzymatic degradation often occurs through a mechanism involving hydrolases. Hydrolases are enzymes that catalyze the breakdown of polymers through hydrolysis, a process where water molecules are used to cleave the polymer chains into smaller fragments and as the results the molecular weight are reduced . It is evident that incorporation of hydrophilic PEG is boosting hydrophobic polymer degradation. The balance between hydrophobicity and hydrophilicity in the polymer structure is crucial for facilitating enzymatic degradation using lipases . Lipases, distinguished by a hydrophobic binding domain and a catalytic active site, engage in heterogeneous enzymatic reactions which involve two pivotal steps: initial adsorption, where hydrophobic enzyme domains adhere to the polymer's solid substrates through hydrophobic interactions, followed by hydrolysis, where ester groups are enzymatically hydrolyzed at the catalytic domains . When exposed to surfaces, the more hydrophobic surface (such as in PBS-DLS copolymer) hinders enzyme adhesion to the polymer film, decelerating the biodegradation process compared to the more hydrophilic surface (PBS-DLS-PEG).