As the new parameters differentiate between lipid tails that vary by two carbons, there must be a meaningful difference in properties between lipids with increasing tail lengths. One of the major experimental differences between saturated lipids is their main gel-liquid transition temperatures (Tm). To quantify Tm from Martini 3 simulations, we conduct simulated annealing simulations (Figure ) of four saturated phospholipids; DPPC (16:0/16:0), DSPC (18:0/18:0), PSM (d18:1/16:0) and SSM (d18:1/18:0), which have experimental Tm of 314 K, 328 K, 314 K, 318 K, respectively . The temperature of the annealing simulations ranges between 273 K and 373 K (Figure ). The simulations start from an equilibrated bilayer at 373 K, quenching the temperature at a rate of 1 K/10 ns, until reaching 273 K, followed by heating returning to 373 K at the same rate. The Lindemann Index 112 was calculated for apolar chain beads in blocks of 10 ns, such that each block corresponds to a change of approximately 1 K: