Chemical methods like the Sanger and Edman degradations appeared decades before DNA sequencing 1,2 . However, the supremacy of current Next-Generation DNA Sequencing techniques outperformed proteomics in terms of throughput, costs, and the minimum amount of sample needed for analysis giving genomics the lead of innovation and downgrading ex-novo sequencing to the research of general properties of proteins. Mass spectrometry (MS), the gold standard for protein sequencing, cannot fulfill the requirements to translate to day-to-day applications covering the irrupting biological discoveries that require protein diagnosis . In the last decade, the research of new methodologies for protein sequencing has intensified to provide wider access to high throughput analysis of smaller quantities of samples . Very significant contributions include combinations of chemical degradations with fluorescence labels and the transduction of selective enzymatic reactions by the detection of light using massively multiplexed metal-oxidesemiconductor circuits . In general, the new alternatives are still in the early stages of development facing serious challenges related to the pivotal task of AA identification due to the overlap of the chemical and physical properties of AAs. Although AA identification has progressed , new methods to identify the AAs are still a necessity to provide an alternative to MS.