The lipid mixture was prepared by dissolving the lipid at the desired amount in chloroform. To facil-113 itate visualization, 0.1 mol% Liss Rhod PE was added to the mixture. The mixture was evaporated 114 by passing a gentle stream of nitrogen for at least 5 minutes. After the chloroform was completely 115 evaporated, 1-octanol was added to achieve the desired concentration. To ensure proper disper-116 sion of the lipids in the 1-octanol, the mixture was placed in an ultrasonic bath machine (MCS-2, 117 AS ONE Corporation, Japan) for a minimum of 10 minutes at room temperature. This ultrasonic 118 treatment aided in the dispersion and homogeneity of the lipids.