Additionally, the hydrolysis of the PEO resulted in the formation of an aldehyde at the end of the PEO fragments. The triplet peak at 5.2 ppm (purple dot) corresponded to the acetal structure, which experienced a favorable shift to acetal of aliphatic aldehyde in water. A tiny singlet signal at 9.6 ppm (yellow dot) presented a minor equilibrium product, the aldehyde. Furthermore, the release of benzoic acid and the formation of PEO fragments led to an imminent reduction in molecular weight, which we monitored over time. The steady liberation of benzoic acid was observed from 39% at 24 h to 87% at 96 h, accompanied by a reduction in molecular weight from 13.4 kg/mol to 3.5 kg/mol at 24 h and 2.2 kg/mol at 96 h. We investigated the potential of modified polyethylene oxide (PEO) as a drug carrier for biologically active molecules such as ibuprofen and glycyrretinic acid. The cytoprotective activity of PEO-ibuprofen (2n-6k) and PEO-glycyrretinic acid (2o-6k) was evaluated using RAW264.7 cells that were stimulated with lipopolysaccharide (LPS) (Figure ). Our results