All experiments were performed on an ultraperformance liquid chromatography (UPLC) system (nanoACQUITY, Waters, Wilmslow, UK) coupled to an electrospray ionization quadrupole time-of-flight (ESI-Q-ToF) mass spectrometer (Xevo G2-XS, Waters, Wilmslow, UK). The standard nanoACQUITY system contains a refrigerated HDX manager with a two-valve configuration, i.e. injection and trapping valve, supplies solvent flow via Auxiliary (ASM) and Binary (BSM) Solvent Managers. The HDX manager was kept at 0 C during all measurements.