Isolation from E. coli E. coli overnight culture (30 mL) was prepared as previously outlined and used to inoculate 3 L of LB media. This new culture was incubated at 37 °C with 180 rpm shaking until reaching an OD of 1.777. Cells were pelleted by centrifuging at 4200 rpm for 45 min, the supernatant decanted, and cells redispersed in 50 mL PB using a vortex mixer (Classic vortex mixer, VELP, Italy). Cells were extracted by centrifugation (15000 × g, 5 min, 4 °C) and washed twice with PB.