Based on criteria of overall antifungal activity, cytotoxicity and haemolysis as well as compound availability, we selected compounds Co1, Ag2, Eu1, Ir1, Pt1, Pt2, Pt4, Pt5, Au1, Au2 and Au4 to conduct a preliminary in vivo toxicity assay. For these initial in vivo assessments, we have recently used the greater wax moth G. mellonella instead of a rodent model. Firstly, it is significantly less resource and cost-intensive, allowing us to screen far more compounds than would otherwise be possible. Secondly, results obtained in G. mellonella have been shown to be robust, reproducible and correlate well with toxicity study results obtained in rodent models. The compounds were dissolved in DMSO and diluted to the highest possible final concentration (Table ).