To build both types of bilayers, we used Packmol to place the monomers in a bilayer consisting of 128 monomers (64 monomers packed into each monolayer) and 1000 glycerol molecules. The initial box dimensions are 6 nm × 6 nm × 10 nm, where the bilayer occupies the middle 5 nm and 500 glycerol molecules occupy each side of the bilayer. This composition, cell size, and cell aspect ratio is expected to favor a single bilayer. We then energy minimized and equilibrated the system using MD. During the equilibration, we maintained a semi-isotropic periodic boundary condition where the box lengths were equal in the x and y directions. We found that both the atomistic and CG models easily stabilized as bilayers in less than 10 ns of simulation. We compared the structural properties, such as density distributions as functions of distance from the bilayer symmetry plane (aligned in the z-direction) and angle and distance distributions between sets of particles of the CG bilayer and the all-atom bilayer to test the accuracy of the CG model.