The oligonucleotides used in this study (listed in Table ) were purchased from Eurofins Genomics after the high purity salt-free (HPSF) purification method and used without further purification. T4 polynucleotide kinase (PNK), T4 DNA ligase, 29 DNA polymerase, dNTP, BSA, and 29 buffer were purchased from New England Biolabs, USA. Tris buffers, NaCl, spermidine, and DTT were purchased from Sisco Research Laboratories Pvt. Ltd. (SRL) -India. SYBR™ Green I Nucleic Acid Gel Stain 10,000X was purchased from Invitrogen. All solutions were prepared using ultrapure water obtained from a Millipore Milli-Q Type II water purification system.