Nevertheless, in view of the results presented in this study, targeting the parasite metabolic pathways using nucleobase conjugates of the trithiolato diruthenium derivatives does not appear as a very promising approach. In the applied in vitro biological activity evaluation, the hybrid molecules presenting nucleic base units did not necessarily performed better compared to conjugates presenting other types of substituents. However, the antiparasitic activity and the toxicity on the host cells were influenced not only by the nature of the molecule appended to the diruthenium scaffold but also by the linker between the two moieties.