The APL was calculated using two methods: 1) by multiplying the box dimensions in the xand y-directions with each other and dividing the result by the number of lipids in one leaflet (APL xy ), and 2) through 2D Voronoi tessellation (APL vor ). Using the latter method allows the determination of individual APL values for each lipid, whereas the former gives only the average APL for the whole membrane with the assumption that the membrane is flat.