showing a value of 63.5, followed by AAV6 with 56.8 and lastly AAV8 with 31.6, which was expected given the visibly broader peaks. Run-to-run variability was evaluated and was found negligible for reten9on 9me with rela9ve calculated standard devia9ons of below 0.1% for full and empty capsid peaks of all three AAVs tested (Table ). Rela9ve standard devia9ons of peak areas were larger, in a range of 1 to 5%. The largest devia9on was observed for AAV6 (4.79%). It should be noted that AAVs are prone to non-specific adsorp9on to solid surfaces which can occur throughout all stages of produc9on and characteriza9on . Materials that were shown to retain AAV par9cles include plas9cs, stainless steel and importantly also glass, the material here used to contain AAV samples in the LC autosampler.