This data was reprocessed using the new approach. Because this data did not have linker-payload cleavage, we skipped the first library building step and directly processed raw data using BE. We added maleimide hydrolysis as a potential modification to linker-payload. Cysteine adduct was added as modification to any unpaired cysteine. The software performed automated deconvolution, peak picking and peak annotation using the sequence information and modifications we provided. Once parameters were setup, this workflow took less than 30 min to finish.