The anionophore activity can be analysed by calculating the initial transport rate and the half-life (or rate constant). When the data from transport experiments are plotted as F0/F, the curves correspond to the chloride concentration inside the liposomes according to the Stern-Volmer relationship. This is in contrast with plotting the curves as F/F0, which allows a visual comparison, but where the y-axis is not linearly correlated to the chloride concentration. Thus, the inverse of the final averaged curves is taken and all data after 500 s are removed (Figure ). The data from 0 to 500 s of the F0/F curve are fitted with a single exponential function: