Unlike to the molar mass characterization of lignin by SEC-MALLS , the laser wavelength of the MALLS detector did not influence the determination of the Rg of LNPs (Table ). The high scattering intensity of the particles outweighs the influence of fluorescence and absorption of the lignin molecules-a major bottleneck in the analysis of lignins by MALLS. However, we would like to mention that LNPs tend to stick to various surfaces (e.g., membrane, flows cells, capillaries), and therefore, may require a more frequent change of the membrane and cleaning of the AF4 system to ensure long term stability and quality of the measurements. and does not allow to fit multiple peaks manually. Since the automatic fit applied by the software may not always be accurate, additional verification is required by the user. Figure shows the difference between the automated MALLS data fitting and the manual fitting for a sample containing a mix of two standards (PS-mix) and individual injections of the respective standards (PS-15 and PS-50). As shown in Figure and Table , there were only minor differences in the slopes and Rg values between the different fit approaches (17 and 48 nm for the automatic fit-1 and fit-2, and 17 and 50 nm for the manual fit of PS-15 and PS-50, respectively). However, it is strongly recommended that the users validate the performance of the automatic fitting to ensure accuracy.