Metabolomics has been instrumental in elucidating the metabolic mechanisms underlying the establishment and functioning of plant-symbiont associations . By comparing the metabolic profiles of symbiotic and non-symbiotic plants, researchers have gained valuable insights into the metabolic adaptations and exchanges that occur during these interactions . For example, a study by used GC-MS and LC-MS to investigate the metabolic changes in barrel medic (Medicago truncatula) roots during the establishment of symbiosis with the arbuscular mycorrhizal fungus Glomus intraradices . The authors identified several metabolites, such as amino acids and organic acids, that were involved in the nutrient exchange between the plant and the fungus and were essential for the successful functioning of the symbiosis. Similarly, a study by used LC-MS to investigate the metabolic changes in the roots of the legume Robinia pseudoacacia during its symbiosis with the nitrogen-fixing bacterium Mesorhizobium amorphae . The authors identified several metabolites, including flavonoids and amino acids, that were differentially accumulated in the roots during the symbiotic interaction and played important roles in the nodulation and nitrogen fixation processes.