Full MS was carried out at a resoluNon se}ng of 120,000 (at m/z 200), with a normalised automaNc gain control (AGC) target of 3 × 10 6 for a scan range of 200-2,000 m/z and a maximum injecNon Nme of 100 ms. Data-dependent MS/MS scans were performed at a resoluNon se}ng of 15,000 (at m/z 200). An isolaNon window of 1.2 Th, maximum injecNon Nme of 250 ms, normalised AGC target of 100% and normalized collision energy of 27% was used. Ions selected for MS/MS fragmentaNon had to have an intensity threshold of 5.0e3 and charge states of between 2 and 7, while monoisotopic peak determinaNon was set to 'PepNde' mode. AddiNonally, MS/MS dynamic exclusion of 7 s was applied, with a mass tolerance of 10 ppm and excluding species aper MS/MS event. MS acquisiNon was set to occur only between 0 and 70 min of the chromatographic gradient to prevent contaminaNon on the MS system.