The selection strategy for the initial seeds, by selecting a random subset from the diverse subset of enumerated compounds from the Enamine REALSpace, to be screened worked fine for all three targets investigated. However, such prepared diverse subsets are not available for all chemical spaces. For example, the largest commercially available non- enumerated chemical space available is eXplore which has over 5 trillion compounds and no readily available diverse subset to sample from. The optimal picking of seeds for such chemical spaces is beyond the scope of this study, but warrants further consideration.