where both Γ and K r are positive quantities (for derivation, see SI section V B4), the latter denoting the ratcheting constant (recently applied in the context of dissipative self-assembly ) which quantifies the kinetic asymmetry of the motor (see Box I). Under the experimental conditions in which the motor was originally operated, the rotary motor is driven purely by information flow as the macrocycle binds with equal affinity to fumaramide stations adjacent to a hydroxy group and an Fmoc group (within the detection limits of 1 H nuclear magnetic resonance (NMR) measurements), so there is no energy flow. However, when the standard chemical potentials of the distal and proximal co-conformers differ, energy flow arises according to equation ( ). An example of this could arise if there was, say, a stabilizing interaction between the macrocycle and the Fmoc group, which would lead to an increase in energy of the macrocycle upon removal of the Fmoc-barrier close to it. As a consequence, the standard chemical potential would decrease, leading to the release of heat, each time net mechanical displacement occurs in the forward direction. This fits the definition of a "power stroke" (see Box I). Therefore, according to equation (2), the energy flow accounts for the part of the fueling free energy that contributes to destabilizing the macrocycle during chemical transitions and which is subsequently dissipated in a power stroke.