The screening of multiple reagents and assay formats resulted in many assay plates with a large volume of data to be processed to enable the selection of the optimal reagents for the PK assay. The scoring function was designed to enable the assay developer to weight parameters differently based on assay requirements. Manually processing this data using a spreadsheet is time-consuming and potentially can introduce errors. In order to streamline this process, the scoring tool was programmed in Python with a graphical user interface (GUI) to accelerate data processing and provide consistency in using the tool (Figure , Supplementary Figures ).