Overall, the first wave of the COVID-19 pandemic and the related shut down had a significant influence on the chemical fingerprint of primary sludge in New Haven, CT. We found upwards trends in hydroxychloroquine and disinfectant concentrations in sludge, reflecting increased use during the initial wave of the COVID-19 pandemic. We also saw increases in drugs of abuse and antidepressants, and seasonal changes for chemicals such as UV-filters that are used in sunscreens. Importantly, we found that benzotriazole concentrations showed different trends during and after the local stay at home order, a key indication that benzotriazole can be used as a marker for the influence of traffic on wastewater and sludge in combined sewer systems. Overall, our findings relate strongly to trends in public and environmental health worldwide and show specific trends that may not have been picked up in other types of analysis.