Figure shows the unsaturated oleoyl (sn-2) tail of POPC. Note that whereas the DMPC carbon segments 3/3', 12/12', and 13/13' allowed Dvinskikh et al. to see the two myristoyl tails separately in APM-CP and R-PDLF, this was not the case for the corresponding POPC carbon segments in our R-PDLF data: The unsaturated/saturated inter-tail carbon pairs 3/3', 16/14', and 17/15' cannot be separated (Fig. ) even though the S CH can be unambiguously assigned as coming from these pairs. The reason for the lack of resolution in our data is the use of a lower number of points in the indirect dimension in comparison to the experiments performed by Dvinskikh. The carbon segments 8-11 around the double bond can, however, be clearly identified in the C spectrum: Their S CH have lower magnitudes than the corresponding segments in the saturated tail (compare Figs. and), and upon dehydration they change less (mean full-to-lowesthydration magnitude change 0.05) than the S CH in the flanking carbon segments 4-7 and 12-15 (0.08).