The extraction process of apple pomace in pilot plant scale to yield about 5 % glycans (purity ≥ 90 %) has been established at Fraunhofer IWKS using subcrictical water. After extraction approx. 70 % of unsoluble apple pomace residue is recovered after removing the excess water by oven drying (80 °C). Apple pomace residue has been prepared as described and processed in a planetary ball mill. The objective of adding mechanocatalytic partial depolymerization to the process chain was to increase the yield of glycans. Therefore, the milled apple pomace was extracted under atmospheric pressure in either hot or cold water. The data for the fractions of solid, soluble and precipitated glycans shown in Figure demonstrate a good reproducibility of the process. The solid fraction was determined gravimetrically while the soluble fraction was the remaining amount left after subtracting the solid and glycan fraction. In both approaches, cold and hot water extraction, a total soluble fraction of 30 ± 1.5 % and a precipitable glycan fraction of about 5 ± 0.5 % could be achieved. To increase the precipitable amount of glycans two changes in precipitation process can be considered. The first is increasing the ethanol concentration in combination with decreasing the precipitation temperature. It has been reported that these parameters influence the precipitation behavior. Short-chain and small branched polysaccharides will be precipitated as well. The second approach would be the intermediate ultrafiltration step as described recently. With this process a concentrated raw glycan solution is achieved which favours polysaccharide precipitation. Figure shows the precipitated glycans from both extractions. While the glycans from cold water extraction are almost pure white, the hot water glycans show a brownish colour. Also a different morphology could be observed.