Sterol modelling followed the same parameterization strategies as those employed to model cholesterol for Martini 3 . Most sterol molecules parameterized in this work share their sterol core with cholesterol, only differing on their aliphatic chains (𝛽-sitosterol, stigmasterol and campesterol). To model each of these a building block approach was used, where the cholesterol sterol core was attached to the corresponding aliphatic chain. Aliphatic chain mapping and bead typing followed standard Martini 3 parameterization guidelines . Chain dynamics and bonded parameters were modeled using reference CHARMM36 simulations of the corresponding sterols [124], both inserted in POPC membranes and in water. To match atomistic reference dynamics, 1 to 2 dihedral potentials and 2 to 4 angle potentials were employed. This is not unlike the strategy used to model the Martini 3 cholesterol model , albeit complicated by the fact that the sterols parameterized in this work have aliphatic chains made up of a larger number of beads.