Studying the effects of TGP-377 on VEGFA protein levels and stimulation of a pro-angiogenic phenotype. Because proteomics and miR-ome data show that TGP-377 is selective at the RNA and protein levels, we studied the effect of TGP-377 on phenotype, i.e., tubule branching density. Indeed, treatment of HUVECs with TGP-377 (500 nM) increased tubule branching density by ~50% (Fig. ). Interestingly, the effect of TGP-377 was similar to that observed when recombinant VEGFA protein (10 μg/mL) was added to the cell culture medium or an LNA antagomir against miR-377 (LNA-377) (Fig. ). In contrast, a scrambled LNA control antagomir (50 nM) had no effect on tubule branching (Fig. ).