Of the cyclic peptides analyzed during physiochemical screening, the lowest and highest recorded retention times were 0.22 and 1.21 min respectively. These retention times formed a non-normal distribution that could be explained by two overlapping Gaussian distributions as seen in Figure . The retention times for the cyclic peptides analyzed during 8 min standard gradient method runs were found to range from 3.37 to 5.29 min. Additionally, the distribution of these measured retention times was sparse across their distribution, with notable gaps displayed in Figure .