Prior to the use in an experiment, RTILs, and ethylene glycol were subjected to drying at 60℃ in a vacuum oven for 12 hrs. The water content of ILs and EG was determined by Metrohm Karl Fisher Titrator (EcoKF Titrator). It was ensured that the water content was less than 0.1% before the experiment. DragonFly liquid handling system was used to create binary mixtures of RTIL and EG in each well across the microtiter plate. After the liquid dispensing step, the 96-well plate was placed in a vibrator (MTI Corporation Ltd.) for 15 min with an evaporation shield to minimize contact with the air. A schematic illustration of the automated mixing of RTIL and EG is shown in Figure .