Epoxidation. In a 500 mL round-bottom flask placed on ice, 100 g of microalgal oil, 14 g of formic acid, and 0.5 mL of sulfuric acid were added while stirring at 200 rpm . 112 g of hydrogen peroxide was added dropwise over 1 hour and the mixture was left stirring on ice for one additional hour, followed by a 30 min rest at room temperature. Afterward, the reaction was placed in a water bath for 2.5 hours at 35 °C. Subsequently, the mixture was placed at room temperature for 30 min, and it separated into two phases. Using a separatory funnel, the mixture was diluted with ethyl acetate and then washed with sodium bicarbonate solution (5% w/v) until it reached pH 7. The organic phase was washed with brine and dried with anhydrous sodium sulfate at room temperature. The solvent was removed by vacuum, to obtain the epoxidized microalgal oil (EMO).