Collectively, these insights support the framework discussed for Figure on degradation mechanisms for electrochemical aptamer sensors and for devices based on self-assembled alkylthiolate monolayers in general. Furthermore, for the first time it appears promising that aptamer sensors will be able to operate for at least one week in-vivo at body temperature. The results reported herein also challenge conventional wisdom regarding self-assembled monolayer stability on gold surfaces and shed important light on several previous conclusions on monolayer stability that were only accurate over shorter term operation of just a few hours. Our final statement is that these results are unoptimized and given further optimization of the surface chemistry, morphology of gold surfaces, incubation, scanning waveforms, and fouling protection, we expect that two week longevity mirroring commercial continuous glucose meters is a fully capturable reality for the field of electrochemical aptamer biosensors.