Quantitative approach to multicomponent di-Ub mixtures. Based on the success of deconvoluting di-Ub pairs, we wanted to extend our mathematical model to more complex mixtures. Whether it is a ternary or higher order mixture, the relative weight of a specific isomer ( 𝑞𝑞 𝑖𝑖 ) should depend on the response factor (𝑘𝑘) of all isomers (Equation ):