To investigate the effect of the fuzzy coat, we utilized a fluid scheme of a single standardised growth phase prior to a shaving step followed by two subsequent growth phases. In all growth phases we inject full length WT α-synuclein. During fibril shaving we observe a fast release of mass, which decays as the bulk of PK-degradable material, the fuzzy coat, becomes increasingly unavailable. The frequency response during monomer incubation, post shaving, is approximately 80% accelerated by frequency and 35% by dissipation and demonstrates the same slow decrease in frequency response as the regular growth experiments. This behavior occurs in both post shaving growth phases, however in the last growth phase the dissipation response is accelerated by 80%. It is not immediately obvious whether the accelerated deposition of mass to the sensor surface originates from monomer binding to the exposed fibril core or accelerated fibril elongation. However, the fibril growth, as measured by the dissipation response, is constant throughout the majority of the measurement. This suggest that the signal is dominated by fibril growth rather than monomer binding, which would be expected to saturate as the shaved fibril surface becomes increasingly covered. In addition, the ratio of dissipation to frequency response during the growth phases is greater than what we find during the shaving phase (approximately -0.3 10 ( -6)/Hz and -0.15 10 ( -6)/Hz respectively), indicating that the material properties of the removed mass by Proteinase K treatment are different from the predominant mass contribution during fibril growth post-shaving. It is reasonable to expect that the material properties of bound monomer would be comparable to that of the fuzzy coat rather than the full fibril, suggesting that monomer binding is not contributing significantly to the response post shaving.