The production and use of micropore device configuration for studying biomolecular electron transport via protein junctions is demonstrated. Its use allows control over contact geometry, reduces contact resistance, and is suitable for permanently contacted devices. A requirement for high yields of such configuration is that protein coverage is dense. If that condition is fulfilled, the MpD approach provides a robust platform for fabricating and analyzing protein-based MpD junctions, offering significant potential for electron transport studies of biomolecular systems.