In summary, we have successfully constructed release systems based on a self-assembling RADA16-I peptide with three signal sequences that can be used to improve wound healing processes. We demonstrated that the hybrids form hydrogels, a β-structure, and fibres, can reassemble, can withstand shear stress, and their gels form a highly ordered structure. They are cleaved at the designated site, releasing the active motif, and can easily be cleared from plasma by hydrolysis. They are not cytotoxic, they stimulate skin cells to grow and they can serve as scaffolds for cells. In tests, the improvement of skin wound healing was most prominent following the application of the RADA-GHK and RADA-KGHK peptide hybrids, which is easily seen both from the wound closure kinetics and histology samples. Interestingly, RADA-IM, despite not stimulating wound closure as extensively as RADA-GHK and RADA-KGHK, stimulates the growth of hair follicles. These newly synthesized peptide hybrids showed great potential in wound healing. However, further research is needed.