The rate and degree of the denaturation process depends on the following interrelated parameters 21 , 22 temperature, structure and size of DNA, the composition of surrounding media (solvents , buffers , pH, salts 26 , 27 , 28 ) . Many theoretical and experimental works have been published to describe factors related to DNA denaturation. However, the precise mechanism of DNA denaturation is still poorly understood and has continued to attract the attention of researchers. 3.1 The theoretical works related to the thermal denaturation of