Cholesterol is a key component of human cell membranes and plays a crucial role in differentiating bacterial and human cell membranes. Its presence, particularly alongside polyunsaturated fatty acids (PUFAs), increases membrane mobility, softens the membrane, and induces lipid condensing and thickening . Noteworthy, RBC membranes, the most studied for membrane composition and AMP toxicity, are indeed rich in PUFAs and cholesterol .