Our incorporation of super-localization microscopy principles into the pseudo-infection model will likely expand the utility of this model to address many general problems in membrane biophysics which deal with the efficiency of transfer of vesicular contents. For example, our method will be useful to interrogate the mechanistic details of agents which are thought to inhibit expansion of fusion pores induced by viral fusion proteins . It will also be possible to interrogate whether viral proteins alone are sufficient for fusion pore stabilization and expansion or whether host cellular factors are also responsible . In this regard, an improvement in photophysical properties of the RNA-binding dye will allow sequential imaging, and it may be possible to study the evolution of content distribution pattern as a function of time. Finally, we foresee that our method of content distribution assay based on super-localization microscopy can be further adopted for estimating the efficiency of endosomal escape of lipid nanoparticles (LNPs) which is still largely an empirical subject . A reliable and straightforward measure of the content distribution between LNPs and target vesicles (mimicking endosome) will likely reflect its endosomal escape behavior and thus facilitate rational design of such nanocarriers.