Recognizing their potential as bioimaging reagents, we next prepared water-dispersible nanoparticles (glassy organic dots, g-Odots) by encapsulating these two red MR-TADF compounds in combination with mCP, surrounded by the amphiphilic polymer DSPE-PEG2k. The g-Odots were formed by heating an oil-in-water emulsion of mCP with DSPE-PEG2k (5:1 molar ratio of mCP:DSP-EPEG2k) and 5 wt% TADF emitters at 180 ℃ in a microwave reactor (see ESI for experimental details). After centrifugation, each fraction of g-Odots was analyzed by nanoparticle tracking analysis (NTA), which revealed that the supernatant after centrifugation at 10,000 relative centrifugal force (RCF) contain particles of mean diameter of ~100 nm (Figure and). Additionally, the polydispersity indexes (PDI) values for the g-Odots were reasonably narrow at 0.117 and 0.176 for PhDPA-DiKTa and MeODPA-DiKTa, respectively. Such narrow PDI values allow the g-Odots to be taken up by cells more uniformly. The size of the g-Odots confirmed that the emitters are encapsulated by the DSPE-PEG2k. The spherical morphology of the g-Odots was verified by scanning electron microscopy (Figure ). The PL spectra and decays of PhDPA-DiKTa g-Odots and