Microtubule dynamics were imaged using DIC microscopy on the Nikon Ti Eclipse microscope equipped with the Andor iXon3 EMCCD camera and the 1.49NA TIRF 100× objective. Imaging was performed for 30 minutes at 32 °C, controlled with an objective heater (Bioptechs). Solutions were pumped through the custom-designed chamber using a syringe pump. A motorized microscope XY-stage allowed cyclic data acquisition from 8 to 10 fields of view, collecting images of each field every 10 seconds. This enabled us to gather data on 20-40 microtubules per experimental condition each day.