Most structures with up to two neighbors per folded segment assembled with good to near-perfect yields without any optimization beyond a standard magnesium screen. Importantly, triplex origami retains many of the methodological benefits associated with scaffolded self-assembly; it allows the use of unpurified and nonequimolar oligonucleotides and follows a simple experimental protocol consisting of a single-mixture reaction. The resulting structures are also straightforwardly addressable via DNA hybridization. Additionally, the increased burden of sequence design associated with a two-base recognition motif has already been carried, and the scaffold sequences presented in this work ensure that experimentalists can design new structures without concern for sequence optimization. Our plasmids are archived at a global commercial supplier and can be shared for purchase upon request. Notably, the cost of such a plasmid is substantially lower than of commercial ssDNA scaffolds.