Blood platelets are the foundation of hemostasis and also contribute to a variety of other normal and pathological processes, including thrombosis, inflammation, and tumor development . The hemostatic function of platelets is closely related to their ability to change physical properties in response to vessel wall injury . The first step of this process is platelet activation, which comprises a series of prothrombotic events, triggered by the increase of intracellular calcium . Abnormal platelet activation is considered as a cornerstone in pathogenesis of atherosclerotic cardiovascular diseases , which are the principal cause of mortality globally. Therefore, it is highly anticipated that in-depth study of platelet activation and development of advanced methods for its assessment could contribute to further progress in cardiovascular medicine.