After the urease inhibition study, the effect of different kinetics parameters including pH, temperature, incubation time, and substrate concentration were analyzed for a better understanding of ammonia reduction. Effect of all parameters was evaluated on urease activity in the presence of urease and nitrification inhibitors like 2-Chloro-6-(trichloromethyl)pyridine ammonium thiosulphate in three different combinations as A1 (1:1), A2 (0.25:0.75), and A3 (0.75:0.25). Based on urease inhibition activity, only one concentration (0.50%) was selected for further analysis of kinetic assessment due to its better urea hydrolysis results.