The purpose of this study was to characterize the interaction properties of neutral PFAS and to enable the prediction of their partition coefficients by determining the PP-LFER solute descriptors. While the most recent definition of PFAS includes even compounds with only one perfluorinated C atom, the focus of this study is on PFAS with (CF2)≥4, as these tend to show characteristic partition properties compared to non-PFAS. Isothermal gas chromatographic (GC) retention times were measured on capillary columns of differing polarity. GC retention data are useful because they are relatively easy and fast to measure and are equivalent to partition coefficients, reflecting the intermolecular interactions between the compound and the stationary phase. The measured GC retention data were combined with partition coefficient data from this study and the literature and used to calibrate the PFAS solute descriptors. The resulting descriptors were used to predict partition coefficients, which were compared to predictions by the quantum chemically based COSMOterm model for validation. Finally, the PP-LFERpredicted partition coefficients were used to create a chemical space plot to graphically compare the environmental partition characteristics of various PFAS and non-PFAS.