Simultaneously, the sensitivity strongly decreases after the usual peak for region 2' and converges at a low level from region 5' onwards (see Fig. ). Overall, the construction of QM regions using the systematic saaPCVA approach leads to a slightly better convergence and lower gPVCA sensitivity behavior compared to the distance-based inclusion of amino acids. This confirms the suitability of saaPCVA for the systematic construction of QM regions.