In human blood, albumin constitutes the largest proportion of proteins, amounting to 50-60% of total blood protein. Synthesized by the liver, albumin is distributed throughout the body via the bloodstream, and in healthy individuals, only a minute quantity may pass through the kidneys into the urine. Human serum albumin (HSA) is a crucial protein in plasma, contributing to the plasma oncotic pressure and regulating fluid distribution in the body. HSA has a remarkable ability to bind to various endogenous and exogenous compounds, acting as a carrier and depot for these molecules. Additionally, HSA is responsible for transporting fatty acids, modifying the pharmacokinetics of drugs, rendering toxins harmless, and providing most of the anti-oxidant capacity in human plasma. HSA is a valuable biomarker for several diseases, including cancer, rheumatoid arthritis, ischemia, and glycemic control. Clinically, HSA is widely used to treat numerous conditions such as hypovolemia, shock, burns, trauma, hemorrhage, and hypoalbuminemia, among others. An albumin test measures the amount of albumin, a protein made by the liver that helps keep fluid in the bloodstream and circulate vitamins and hormones, in the blood plasma. The test is ordered by healthcare providers to check for liver or kidney issues. The purpose of detecting albumin levels is to diagnose various conditions related to kidney or liver disease, malnutrition, and dehydration.