The whole study plan was alienated into two phases. In the 1 st phase, the effect of combined inhibitors (2-Chloro-6-(trichloromethyl)pyridine + ammonium thiosulphate) was evaluated on urease activity in rice crops of selected three districts (Faisalabad, Gujranwala, and Sheikhupura). While, in the 2 nd phase kinetics parameters, including the effect of pH, temperature, and incubation time on urease activity were investigated. Different concentrations of inhibitors such as 0.10, 0.25, and 0.50% were blended with urea granules and were applied to rice crops. In addition, three different combinations such as A1, A2, and A3 of both inhibitors including 2-Chloro-6-trichloromethyl pyridine (nitrapyrin) and ammonium thiosulphate (ATS)