AF2 assesses its confidence in each amino acid prediction by the "predicted local-distance difference test" (pLDDT). The pLDDT metric ranges from 0 to 100, with higher values reflecting greater confidence in predicted structures. Typically, pLDDT values above 90 indicate a reliable prediction. Correctness of domain alignments for GRLMR and SeR were also assessed with the "predicted aligned error" (PAE) metric, which is standard practice for multimeric proteins. Low PAE values, indicating relatively high prediction correctness among alignment positions, are ideally within the 0-10 Å range (blue in Figure ). On the other hand, higher values above 15 Å (red in Figure ) would indicate low inter-domain sequence quality.