In light of the strong connections between DOX-induced cardiotoxicity and oxidative stress, H2O2 in body fluids could be used to measure oxidative stress status during physiological and pathological conditions . Clinical studies have not documented H2O2 amounts in DOXinduced cardiotoxicity conditions. Efforts to document H2O2 levels in tumor tissues are limited by its low concentration, short half-life, and high chemical activity . Regular monitoring of H₂O₂ will allow for early detection of cardiac injury before irreversible damage occurs. Centralized laboratories techniques like spectrophotometry , chromatography , Surfaceenhanced Raman scattering (SERS) , chemiluminescence and fluorimetry have historically been the gold standard for chemical and biomolecular analyses. However, the healthcare landscape is rapidly evolving due to social, economic, and technological transformations, with a focus on making diagnostics accessible, improving health standards, and ensuring timely interventions. Despite these advancements, many diagnostic technologies remain inaccessible in low-resource settings, prompting the WHO to establish the ASSURED criteria for global diagnostic platform development . In response to these challenges, technological developments in recent years have witnessed a paradigm shift towards lab-onchip (LOC) or point-of-care (POC) devices for various diagnostic applications. Among these innovations, fluorometric and chemiluminescence techniques have been explored for detecting H2O2 levels in vitro. However, the low sensitivity of these techniques, coupled with the complexity of sample preparation, makes real-time and rapid measurements . Given these limitations, an electrochemical biosensor offers a superior alternative, providing higher sensitivity, simpler sample preparation, and faster, real-time detection of H2O2 . Electrochemical biosensors are cost-effective and portable, aligning with WHO's ASSURED criteria for low-resource LOC or POC diagnostics. However, electrode performance limits sensitivity and detection. Researchers are enhancing electrodes with nanomaterials, metal nanoparticles, and conductive polymers to improve catalytic efficiency and electron-transfer capabilities, offering a viable alternative for improved detection sensitivity and selectivity. Modifying electrodes with appropriate modifiers has proven a viable alternative for improving detection sensitivity and selectivity . Remarkable examples of nanomaterials based electrochemical detection of H2O2 have been reported in the literature. For instance, Wang et al. detected H2O2 by modifying the electrode by AuNPs/ZIF-8@ZIF-67/GCE with high catalytic activity, with a wide detection range of 0.05-120 μM and low limit of detection (LOD) 0.016 μM . Another study, Li and colleagues developed an electrochemical sensor using rROGO-S-Au HS/GCE for H2O2 detection. The sensor has a wide range, low detection limit, and high sensitivity, making it promising for cancer diagnosis and real-time monitoring of tumor progression, with a LOD of 5 μM . These reports demonstrate remarkable nanomaterials-based electrochemical detection capabilities of H2O2 detection capabilities that reduce the fabrication complexity, thereby decreasing overall cost and enhancing scalability.