Moreover, these designs showcase sophisticated physical manipulation of the dsDNA scaffold by inducing global curvature. The bent dsDNA backbones contain no nicks that could promote local flexibility and base fraying shown thus far, the length of dsDNA spacers in the arch varies per folded segment: respectively two, three, and four duplex turns for the inner, middle, and outer segments. This incremental insertion of 42 additional base pairs per segment bends the bundle 180° over an approximate 15 ± 3 nm inner radius. Notably, the good yield of the arch (64%, n = 81) indicates that five TFO staples can bear mechanical loads quite well. An even steeper global curvature of r = 7 ± 2 nm is induced by eight TFO staples in the horseshoe and tilde designs (Figs. and). Herein, the lengths of both tsDNA as well as dsDNA domains are varied. The horseshoe continuously inserts 32 additional bt and 49 additional bp from inner to middle to outer segment, thereby incrementally increasing segment length by 26.7 nm and bending the bundle roughly 300°. The tilde adds mostly the same insertions, but switches the domain lengths of the inner and outer segments halfway through. All three folded segments thus have the same total length and the tilde inverses bending angle at its midpoint. While the yields of some of the curved structures, such as the tilde and horseshoe, are poor, it demonstrates how the curvature of the patterned ts/ds DNA structures can be quantitatively controlled.