For this concentration range, the data from both methods can be readily described with our adsorption model and a linear equation (Figure ). To compare the both approaches, it is convenient to plot the response by normalizing the fluorescence to 0 and 1 and normalizing the absorption to 1. When plotting the response of both methods (Figure ), one observes a clear signal amplification in the nanosensor read-outs. Dividing the NIR read-out by the absorption read-out yields an enhancement factor of ≈ 2.5. The maximal signal enhancement is centered around the enzyme concentration causing 50 % of the response (4.35 ± 0.49 pM HRP), which illustrates that SENSAT depends on the affinity for the analyte. This amplification is smaller than the one observed for PPD but we underline that the basic concept of SENSAT remains valid and optimization of reaction conditions could further improve it.