When collecting blood sample animals are humanely restrained in a cattle chute or head-gate suitable for the blood collection procedure. When blood is collected from the jugular vein, a suitable halter is used to hold the head to one side allowing access to the right or left jugular vein. When blood is collected from the tail vein the restraining equipment will be appropriate to allow access to the tail in an elevated position exposing the tail vein collection site. Appropriate cleanliness and blood collection site preparation will be done per the veterinarian's directive. 10 ml Vacutainer™ EDTA (anti-coagulant) blood collection tubes were used to collect blood from each animal either via Vacutainer™ technique or separate sterile syringe and needle with blood transfer to the Vacutainer™ tube. Blood tubes with anti-coagulant were gently rotated at least eight times for thorough mixing. All blood tubes were placed on ice, or suitable refrigeration, after collection. Processing of the blood sample to collect plasma was done within 4 hours of collection. Samples were centrifuged at 1600 g, 4 0 C for 15 minutes and plasma was transferred to 1.8 ml cryo-vials and placed in storage at -80 0 C until assayed.