On the other hand, the population of neo-neo solvent mediated pair shows different trends: urea-water ~ urea-water-TMAO> water> water-TMAO. Lee and van der Vegt showed that urea sits in between neopentane molecules hence the population of solvent mediated pair is enhanced in urea-water compared to water. For the same reason the populations in water-urea-TMAO and in water-urea are comparable. For both force field of TMAO, it is observed that the population of solvent mediate pair is lowest in water-TMAO mixture.