Freshly transformed clones were used to inoculate 150 μL of LB media supplemented with 50 μg mL -1 kanamycin in Corning®Costar®96-well microtitre flat bottom plates, covered with a BreathEasy film and incubated overnight at 30 °C, 80% humidity at 850 rpm. 20 μL of overnight culture was used to inoculate 480 μL TB autoinduction media supplemented with 50 μg mL -1 kanamycin and 0.5 mM delta-aminolevulinic acid in 96deep well plates and further incubated under the same conditions overnight. Cells were harvested by centrifugation at 4000 rpm for 20 min. The supernatant was discarded, and the pelleted cells were stored at -80 °C. The pelleted cells were resuspended in 200 μL lysis buffer (100 mM KPi pH 7.0, 1 mg mL -1 lysozyme, 0.5 mg mL -1 polymixin B and 10 μg mL -1 DNase I) incubated for 2h at 30 °C, 80% humidity at 850 rpm. Cell debris was removed by centrifugation at 4000 rpm for 30 min. 50 μL clarified lysate was transferred to SureSTART WebSeal Plate+ (Round U-Bottom) 96-well microtiter plates, containing 50 μL of reaction mix (100 mM KPi buffer pH 7, 100 mM glucose, 1 mM NADPH and 2 mg mL -1 GDH with 30% MeOH). Reactions were incubated for 2 h at 30 °C, 80% humidity, 850 rpm and quenched by the addition of 100 μL MeCN. Samples were further incubated for 10 min and precipitated proteins were removed by centrifugation at 4000 rpm for 30 min with the supernatants analysed by UPLC analysis.