Data-dependent acquisition (DDA)-MS analysis was performed in positive ion mode. Full scans were acquired at a resolution of 70,000 between a mass range of m/z 200-2000. The AGC target was set to 1.0 × 10 6 with a maximum injection time of 100 ms and 1 microscan. MS/MS (MS 2 ) fragment scans were acquired using a resolution setting of 17,500 with an AGC target of 1.0 × 10 5 , a maximum injection time of 200 ms, an isolation window of m/z 2.0, and a signal intensity threshold of 1.0 × 10 4 .