While the model describes thermodynamics, kinetics and mass transport phenomena, slight deviations between experimental and simulated CPE traces can be attributed to H2 bubble growth and break-off (Fig. ), contributing to film loss through enzyme desorption, inactivation and reorientation . While DvH [NiFeSe]-H2ase is less susceptible to H2 inhibition than standard [NiFe]-H2ase , it could lose catalytic activity in the presence of high concentrations within the confined space of the macropores. COMSOL simulations revealed a local H2 concentration of 35 mM sufficient to inhibit H2ase (Fig. ) .