After the first stage of equilibration, all simulations were continued unconstrained for 1.5 to 2 µs (Table ). The simulation temperature was kept at 288 K, which is below the experimental T m of both MMG-1 (332.15 K) and MMG-6 (291.15-295.15 K). 11 Lipids and water were coupled separately using the v-rescale thermostat 36 with 0.1 ps time constant.