Determination of the sensitivity of the biosensor to a conserved target enteroviral nucleic acid sequence was recorded using both changes in absorbance intensity and wavelength shift using a Tecan (Spark 10M) plate reading spectrophotometer. The sensitivity was determined by producing log10 dilutions of conserved target enteroviral nucleic acid sequence, from 200 μM to 2 pM followed by addition of equal volumes of gold-aptamer-nanoconstructs to produce a final concentration range from 1x10 -3 M to 1x10 -14 M. All measurements were conducted in triplicate.