We have developed a general, stable, and cytocompatible method of chemical conjugation on the live cell membranes and confirmed the conjugation by super-resolution imaging. One side chain of our cargo molecule is a positively charged ammonium moiety, which provides electrostatic binding resulting in non-covalent conjugation with the cell surface; while the other side contains a phosphate group bearing ADP moiety that forms phospho-ester covalent conjugation with the cell surface phosphates. This dual conjugated modification strategy resulted in long-time stability with the cell membrane and was non-toxic to live cells. While such an approach of chemical conjugation has many applications, we have shown an application of this method for live cell membrane imaging with long-time stability, recovery of the reagent after imaging, and reusability of the reagent for membrane imaging. Also, we have exploited this method for modulating cell-cell interaction leading to an increase in T cells in culture. Our conjugation method can be directly used to modify the live-cell surface functionalities without using additional cell-surface docking steps allowing for an easy-to-use reagent that can be simply adopted in any protocol. Also, no toxic effect was observed as revealed in the metabolic activity of the surface conjugated T cells. Therefore, our simple but powerful cell-functionalization strategy could be useful for cellbased therapeutics with wide-ranging applications. Future work of this technology includes enhancing the function of primary cells for future therapeutic applications. Given the general conjugation chemistry exploited by our method, we expect several other applications of this dualconjugation chemistry to functionalize membrane viruses,