The thiol-ene photopolymerization has also been applied to photocrosslink HA hydrogels and bioinks. In recent work, Dhand et al. combined 0.6% wt MA-functionalized HA (HA-MA) with 4.5% wt NB-functionalized HA (HA-NB) and a dithiol crosslinker to form interpenetrated hydrogel networks in a one-pot crosslinking step involving simultaneous and independent polymerization of the two functionalities. In combination with 17 mM LAP as photoinitiator and 1 M tartrazine as photoabsorber, they printed 20 layer scaffolds with a layer thickness of 100 µm and a xy resolution of approx. 300 µm at exposure times of 2.5 s per layer by DLP. Galarraga et al. used a 5% wt HA-NB formulation containing 40% NB functionality and DTT as crosslinker at thiol:ene ratio of 1, 1.7 mM LAP as photoinitiator and 1 mM tartrazine as photoabsorber. 20-layer scaffolds with 100 µm layer thickness and a xy resolution of 500 µm were obtained using 6 s exposure time per layer. When mixed with 2 mM thiolated RGD peptide and bovine bone marrowderived mesenchymal stromal cells, DLP printed scaffolds showed a >80% cell viability after 3 days, demonstrating the cytocompatibility of HA-NB hydrogel. While the above studies provide insight on thiol-ene HA hydrogels, none of them quantifies their advantages for DLP printing vs. HA-MA beyond the reduction in the required light dose. Here, we compare the precursor stability and reaction kinetics of thiol-ene photopolymerizable HA-NB and AE-functionalized HA (HA-AE) and radical photopolymerizable HA-MA precursors with similar functionalization degree Fene. We quantify the advantages that can be expected from thiol-ene functionalized HA precursors and corresponding thiol-ene HA based photocurable hydrogels and show the resolution that can be achieved in DLP with HA-NB based systems.