h-cisPT is thought to assemble into a tetrameric complex of two heterodimers(PDB: 7PB1), combining DHDDS together with NgBR. As each heterodimer are identical, we are focusing our simulation efforts on a single heterodimer, represented here in a darker shade of blue and green. Recent structures have also had substrate present, represented here with spheres for the resolved heavy atoms (gray for carbon, red for oxygen, yellow for sulfur, and brown for phosphorus), which can inform how readily substrate partitions into the membrane. The h-cisPT heterodimer (cartoon) is placed above a bilayer (van der Waals) with a similar composition to the plasma membrane, and solvated in water (clear surface). Since we want the bound pose to be as unbiased as possible, the proteins in each structure have a different face towards the membrane surface initially.