ROS and RNS are regarded as biomarkers in DILI, which has been detected by various fluorescent probes. Previous studies confirm ATP production is decreased via APAP. APAP-induced hepatoxicity was chosen as the model for ATP-LW to investigate whether the two species can be regarded as early diagnostic biomarkers. Treatment of HL-7702 cells with APAP produced a marked increase in fluorescence in the green channel and significant decrease in fluorescence in the red channel (Fig. ), indicating upregulation of intracellular ONOO -and depletion of ATP after administration of APAP, and illustrating the ability of our probe to detect concentration changes of the two biomarkers by one-photon confocal imaging in DILI. This was further confirmed using NAC, which can increase cellular concentrations of GSH. GSH is capable of eliminating ONOO -and as such has been used to help treat an APAP overdose. Therefore, as expected upon addition of NAC, the fluorescence intensity in the green channel decreased and red channel increased (Fig. ).