Herein, we present a novel biocompatible wearable electrochemical sensor for glucose monitoring in ISF. Compared with previous glucose sensors, our study introduces an innovative combination of 3D-printed HMNs and SANs to address the key limitations in existing technologies. HMNs are fabricated by biocompatible resin, allowing minimally invasive sampling for ISF and ensuring safe interaction with the skin without irritation and toxicity concerns. The incorporation of SANs significantly boosts the sensitivity and selectivity of electrochemical sensing, offering a wide linear range of 0.1 μM to 40 mM for glucose. The integration of 3D-printed HMNs and a finger-activated pump demonstrates the ISF extraction capability in a biocompatible and minimally invasive approach, which is validated through the sampling of artificial ISF from skin-mimicking phantom gel "in vivo". Furthermore, the sensor is coupled with a potentiostat, enabling real-time and wireless data transmission to a smartphone app to provide immediate results of the glucose levels.