Probing the absorbance of the colored product via spectrophotometric assay or analyzing the color intensity of the product via color analyzers can be utilized for estimation of enzyme-like activity of nanozymes as well as can be used as analytical response for quantification of several analytes . The wide application of nanozymes compared of the natural enzymes is maybe due to the characteristic limitations of native enzymes for instance pH and temperature instability, short storage time, no reusability, and highly expensive production methods. Notably, to fix drawbacks of native enzymes, there is two protocols; (i) immobilization of enzymes and (ii) developing nanozymes.