We initiated the synthesis of selenium oxide nanoparticles by following a meticulous procedure. Two 500 ml beakers (labeled as A and B) were prepared, ensuring they were free from any contaminants by washing with a mixture of ethanol and distilled water. In beaker A, 10g of CH3COOH was accurately weighed and dissolved in distilled water to reach a volume of 100 ml. Meanwhile, 10g of Selenium crushed powder was introduced to beaker A, leading to the formation of a dark suspension. Beaker B was employed to prepare a solution of 10g Sodium Hydroxide in distilled water. The next steps involved subjecting the solution in beaker A to sonication at 80°C for 10 minutes, followed by magnetic stirring at 30.6°C and 1160 RPM for 20 minutes. During this stirring process, the solution from beaker B was slowly introduced into beaker A. The resulting mixture was stored in the shade overnight, wrapped in aluminum foil. The next day, it appeared as a microscopic suspension of blackish particles in a liquid dispersion medium.