For the paired agent imaging experiments, the same procedure was used to induce inflammation with N=2 mice. At 1.5 hours after the LPS and saline treatments, each mouse was injected with a 100 μL dose containing a binary mixture of ICG and ICB (200 nmol/mouse, 100 nmol of each dye). Each mouse was imaged using the ICG and ICB filter settings described above with a 1 sec interval between the image capture at 0, 1, 2, 3, 4 hour(s). At 4 hours the mice were anesthetized and sacrificed by cervical dislocation and imaging was used to ascertain biodistribution and to quantify dye accumulation in the inflamed tissue.