MNPs internalization was studied by fluorescence microscopy (using MNPs modified with TAMRA fluorophore) and ICP-OES. Fluorescence microscopy. To perform fluorescence microscopy analysis, 5 × 10 4 cells were seeded on a glass 4-well chamber slide. After 24 h of culturing, cells were treated with 100 μg Fe/mL of MNPs functionalized with glucose and incubated at 37ºC (5% CO2) for 24 h.