According to the generated PLIFs, the median number of protein-ligand interactions observable among the 2501 measured protein-ligand complexes was 11, with substantial variation across the ligands and targets (Figure ). In comparison, the median number of proteinligand interactions identified among the docked poses of the active compounds of DUDE-Z was 8 for Vina and 7 for DiffDock-L (Figure ). The lower number of protein-ligand interactions observed for the protein-ligand complexes obtained from docking may indicate technological limitations. It is plausible that detecting some of the protein-ligand interactions would require considering the conformational changes induced upon ligand binding to the target protein. We then investigated whether the generated docking poses resemble the protein-ligand interaction patterns of measured protein-ligand complexes. To obtain informative results from this experiment, we prefiltered the 2501 measured protein-ligand complexes for those forming at least three interactions according to their PLIFs. This filtering step reduced the number of measured protein-ligand complexes to 2425.