The temperature was controlled by a dry bath for samples, and the cuvette temperature was maintained by using a temperature-controlled water pump system. The resulting fluorescence spectra and the (QE x C) versus C plots were given in SI, Figs. S30-S34. The apparent Kds and fitting parameters were summarized in SI, Table . The calculated Kds at different temperatures were then combined with Van't Hoff analysis to derive the binding thermodynamic data. By integrating the two Gibbs free energy equations (Eqs. 6 and 7), the resulting changes in the MLGI enthalpy (ΔH) and entropy (ΔS) terms can be obtained from the linear fits of the In(Kd) vs. (1/T) plots using Eq. 8. ∆𝐺 = -𝑅𝑇𝑙𝑛(𝐾 𝑎 ) = 𝑅𝑇𝑙𝑛(𝐾 𝑑 ) (Eq. 6)