The proof-of-concept experiments presented here show the viability of this TLC cartridge technique which can be adapted to develop a system that can completely automate this cartridge-based analysis, opening the possibility of using this cheap and simple technique on an individual patient at different time points to determine elimination and metabolization rates for the drug of interest. Future efforts will develop an automated system to manage cartridge manipulation and fluid handling. This will lend itself well to the bedside clinical environment reducing the physical distance and time required to move the blood sample from the patient to the analysis device. This reduction is critical to prevent degradation of the drug in whole blood samples which can compromise the accuracy of quantification measurements. This would also minimize the amount of effort from the medical staff to perform the analysis making it more likely to be successful in clinical translation.