Reversed-phase preparative HPLC was carried out using Shimadzu SIL-10 AP and an LC-20AP pump system with a UV detector at "The Bangalore Bio Innovation Centre". The separation was performed on a Sunfire C18 column. UV detection was carried out at 254nm with an injection volume of 58.07 ml. The mobile phase was consisting of methanol as solvent A and water containing 0.1% TFA as solvent B. The gradient program set in the software is shown in Table .