Lipids and amphiphilic block co-polymers are common materials for fabrication of synthetic membranes, in the form of lipid vesicles and polymersomes, respectively. Although lipids are biocompatible, the major drawback of liposomes is their rapid aging caused by the formation of transitory pores resulting in high permeability and their labile structure can lead to low mechanical stability and, eventually, aggregation. Polymer membranes are more colloidally stable and robust and, in general, can be designed to have low permeability. While pure lipid or polymer systems have successfully been used to demonstrate biotechnological applications of vesicles, blending of these constituents into hybrid structures offers the potential for greater tuneability with the ambition of synergistically combining their best features. Therefore, hybrid lipid-polymer vesicles aim to take advantage of the robustness and chemical versatility of a polymer membrane as well as the biocompatibility and softness provided by a lipid bilayer. While a variety of block copolymer chemical structures have been blended with phospholipids, polybutadiene-poly(ethylene oxide) (PBd-PEO) block copolymers are one of the most commonly studied examples. PBd-PEO containing hybrid vesicles may have potential applications in nanomedicine and the augmentation of membrane proteins for biotechnology applications such as targeted drug delivery, sensors or artificial cells. Focusing on membrane protein biotechnology, PBd22-PEO14 has been blended with POPC lipids for the reconstitution of the membrane enzyme cytochrome bo3; all hybrid compositions studied were found to maintain > 40% of their initial reconstituted protein activity after 41 days storage at 4 °C, a significant improvement over purely lipid vesicles. Further study revealed that 50/50 mol% PBd22-PEO14/POPC hybrid vesicles retain ~20% of their initial activity after 500 days storage, where the rate of decay in enzyme activity is approximately 5 times slower than for proteoliposomes. Other membrane proteins have been successfully reconstituted using PBd-PEO hybrid vesicles. PBd22-PEO14 blended with E.coli extracted lipids has been used to successfully reconstitute two ATP binding cassette membrane proteins, P-gp or NaAtm1. Combinations of membrane proteins have also been successfully reconstituted into PBd-PEO hybrid vesicles: F0-F1 ATP synthase and bacteriorhodopsin in 50 mol% PBd22-PEO14/POPC hybrid vesicles retained > 50% of their activity after 42 days. Besides enhanced durability of protein function, block copolymers have been shown to enhance membrane protein folding into hybrid vesicles: up to 25 mol% of PBd22-PEO14 or PBd12-PEO9 in DOPC hybrid vesicles enhanced MScL protein folding during cell-free expression. Therefore improved understanding of the physicochemical properties of these hybrid membranes should further enhance their optimisation for membrane protein biotechnology.