The LC-MS/MS data of the microalgae and serum samples was processed as well as the oxPUFA samples with MZmine3 using the same parameters. The features were aligned for each type of sample resulting in two pairs of feature tables and the MS/MS files for the serum and microalgae samples, respectively. Then, a classical molecular network was constructed employing the extracted MS/MS files from the serum, microalgae, and the 'all oxPUFA' alignment described above. The networking parameters remained consistent with those used for the feature-based molecular network, with the addition of the 'MS cluster OFF' option and library matching against both GNPS and our NEO-MSMS libraries.