The authors also emphasize the importance of optimizing algorithms for peptide identification, such as spectral library searches and the prediction of spectral features, to better suit the unique characteristics of single-cell proteomics data. Hence, it is reasonable to assume that the number of identifications following post-processing with MS 2 Rescore can be further increased with more specialized models designed for single-cell data or by including single cell-specific features in the rescoring process. Additionally, there may be less initial high quality target PSMs available to the rescoring engine to train its scoring model.