Thermoresponsive polymers exhibit reversible phase transition in solution upon temperature changes. For thermoresponsive polymers exhibiting lower critical solution temperature (LCST) behavior, phase separation and aggregate formation in solution are triggered above a critical temperature. The temperatures at which phase separation occurs are defined as cloud points (Tcp) because phase-separated solutions show a turbid appearance. In aqueous polymer solutions, the LCST phase transition is primarily driven by entropy loss related to hydrogen bonding interactions. Entropy loss becomes dominant upon heating, results in the dehydration of the polymer and phase separation LCST behavior can be tuned by modifying the hydrophilic character of the polymers through copolymerization with hydrophilic or hydrophobic co-monomers or through end-group modifications. Poly(2-oxazoline)s (POx) are polymers with tertiary amide repeating units, synthesized by cationic ring opening polymerization. This synthetic polymer family has recently received increased attention, as they have been found to have a wide scope of potential applications, including surface coatings, indirect food additives, drug delivery systems, and hydrogels. Recently, a POx-based polymer-drug conjugate successfully completed phase I clinical trials, and a POx-based hemostatic patch was approved for clinical use. The water solubility of POx homopolymers mainly depends on the length of the alkyl groups of the sidechain. While poly(2methyl-2-oxazoline) is completely soluble in water, poly(2-ethyl-2-oxazoline), poly(2-n-propyl-2oxazoline), poly(2-iso-propyl-2-oxazoline) and poly(2-cyclo-propyl-2-oxazoline) show thermoresponsive behavior with LCST. One of the most studied homopolymers belonging to the POx family with an LCST is poly(2-ethyl-2-oxazoline) (PEtOx) with a Tcp above 60 °C in water.