The cytotoxicity study was carried out according to ISO10993-5 using extract materials and the L292 cell line. Briefly, a 96-well plate was used to culture 10 x 10 3 of L292 cells in each well using complete growth medium (Dulbecco's Modified Eagle Medium (DMEM), containing 10% Fetal Bovine Serum (FBS), 2 mM Lglutamine, 100 U/mL penicillin and 100 µg/mL streptomycin). The cells were incubated for 24 hours at 37 °C in a CO2 incubator. At the same time, samples films (0.5 mm thick) with a surface area of 3 cm 2 were prepared, cut into small pieces and placed in a 24-well plate. They were incubated in CO2 incubator over 24 hours at 37 °C in 1ml of complete growth medium. Commercial, non-toxic poly(ε-caprolactone) (PCL, Capa™ 6430) was used as a negative control, nitrile glove (Mercator Nitrylex Classic, Kraków, Poland) were used as a positive control, no material as a control and media in empty well as a sham. After 24 h, the cell culture medium was aspirated from the plate and replaced with 100 μL of extract medium from a given sample (5 technical replicates; 3 samples per material). The plates were then returned to the cell culture incubator for a further 24 hours of culture.