The highest urease activity of about 21.09, 23.94, and 18.21 IU/g soil was noted for Gujranwala soil as compared to Faisalabad and Sheikhupura cultivated soil samples with A1, A2, and A3 combinations at 30 min incubation time and 0.50% concentration. Results reported that with increasing the incubation time the urease activity also increased. However, the minimum hydrolysis of urea was observed at 5 min incubation time. The activity in control (untreated) samples was recorded high as compared to inhibitor-treated samples, because of the production of more urease from plants and micro-organism due to substrate stimulation. Sha et al. stated that 336 h incubation of urease inhibitor (NBPT) significantly reduced the cumulative HN3 loss from soil as compared to control (alone urea). They also performed an experiment with mixing the urea with DAP fertilizer and amended with inhibitor, they found that the coating of urea with DAP reduced the overall efficiency of inhibitor after 84 h incubation time.