The selectivity of the proposed glucose biosensor (Au/Pt-B/o-PD/β-CD/GOx) was evaluated by investigating the impact of potential interfering substances. The substances that could potentially interfere with the sensor's IDEs response, either through direct electrode oxidation or reduction, or direct enzymatic interactions, were examined. Common interfering compounds found in saliva, such as AA, UA, D-Fruc, and Ace , were considered due to their potential to affect the accurate detection of glucose concentrations in saliva. Per-and polyfluoroalkyl substances (PFAS) molecules, such as Nafion, are used in glucose sensors due to their ability to improve the selectivity and stability of the sensor. However, these molecules can influence the performance and reproducibility of the sensor because they are sensitive to changes in humidity and temperature. Additionally, they can decrease the sensor's sensitivity and response time by obstructing the glucose diffusion to the electrode surface. Moreover, they can present environmental and health hazards as these forever chemical persist and bioaccumulate in the environment and living beings (Teymourian et al., 2021). Therefore, alternative material, such as β-CD was used in GOx modified electrodes because of the cone-shaped β-CD molecule has a hydrophobic (water-repelling) inner surface and a hydrophilic (water-attracting) outer surface due to the presence of hydroxyl groups. This allows the β-CD to encapsulate a "guest" molecule, such as glucose, within its hydrophobic interior. Meanwhile, the hydrophilic exterior of the β-CD molecule makes it soluble in the aqueous phase, which is particularly useful when testing saliva. This structure effectively blocks interference from other substances present in saliva, thereby enhancing the selectivity and accuracy of glucose detection . Analyses were conducted on 10 mM buffered solutions containing 2 mM glucose (5 mg.ml⁻¹ GOx) with the addition of known interferents and other sugar at concentrations higher than their normal physiological levels. Fig. displays CA measurements for a 2 mM glucose concentration, along with a 2 mM glucose concentration containing 0.5 mM each of AA, UA, D-Fruc, and Ace. The CA responses were recorded at -0.25 V for 800 s for all four interfering species. The initial 400 s were dedicated to the 2 mM glucose concentration without any interferents added to the system. The spikes at 400 s arises from opening the faraday cage and represent the addition of the interfering substances into the 2 mM glucose concentration, and the responses were recorded until 800 s. The figure indicates minimal current differences between the glucose current and the current of the interfering species. The measured current for each interfering substance (Io), in the presence of the measured glucose current (I), is expressed for clarity as a percentage of (Io/I × 100). The results displayed in Table demonstrate interfering effects without and with different concentrations of β-CD (2.5 to 15 mM). It can be observed that the interferences from 0.5 mM AA, UA, Dfruc, and Ace in 2 mM and 5 mM glucose concentrations with 15 mM β-CD are almost negligible, whereas the absence of β-CD has shown the highest interference effects. The CA responses of with and without β-CD were also depicted in Fig. . Thus, Au/Pt-B/o-PD/β-CD/GOx modified electrodes demonstrate anti-interference ability to glucose detection against common interferent species that normally coexist with glucose in biological matrices, such as saliva. Additionally, the stability of the Au/Pt-B/o-PD/β-CD/GOx modified enzymatic based glucose sensor was investigated by CA method. Subsequent to fabrication, a sensor was stored at 4 ºC for a duration of 85 days in a 10 mM PB, and the change in current response toward 4 mM glucose concentration was measured as shown in Fig. . Initial observations indicated a lower signal from the proposed sensor during the early days, possibly attributed to stabilization or enzyme activation, which might necessitate a brief period for optimal responsiveness. Nevertheless, the sensor provided a RSD of less than 4% for this study, indicating its robust stability. These results affirm the applicability of the developed sensor for glucose detection in real samples. To extend the shelf life of the electrode, considerations for proper storage conditions such as an inert atmosphere, low temperature, low humidity, low-pressure atmosphere, or vacuum may be necessary.