IgG could be produced by HEK or CHO cell lines. However, CHO cell lines only produce α2,3-linked sialic acids while HEK cell lines could produce both types of linkage . As such, identification of the sialic acid linkage is crucial for quality control in the pharmaceutical industry. However, conventional LC-MS struggles to distinguish between α2,3 and α2,6 linkages since they share the same molecular weight and result in identical m/z values . Having the ability to distinguish linkages with our platform will significantly benefit the pharmaceutical industry and the glycobiology community.