To validate the integration of mRNA with ZIF-8, a comprehensive analysis involving zeta potential measurements, energy dispersive spectroscopy (EDS), and a molecular probe assay was conducted. Zeta potential assessments were carried out for individual components, as well as the composite mRPZ and mRNA-PEI nanoparticles (Figure and). The binding of PEI with mRNA to form mRNA-PEI (27.3 mV) resulted in a discernible reduction in zeta potential compared to PEI alone (43.1 mV). Subsequent coating with ZIF-8 led to a significant increase in the zeta potential of the particle composite (mRPZ), reaching 59.5 mV, which is greater than ZIF-8 alone (45.63 mV). This observation provides compelling evidence of the incorporation of mRNA-PEI within a ZIF-8 shell.