Though quantitative decomposition rates were not available, we achieved qualitative accuracy in ranking the stabilities of B1a, B1b, D1a, F1a, and F1b from Tan et al. with 100 mM NCB concentrations. Due to high correlation between ∆G and ∆G ‡ , as well as qualitative agreement 121.2 kcal/mol (-591.0 to -507.2 kJ/mol). The majority of our library shows increased stability when compared with a common mononucleotide NCB, nicotinamide mononucleotide (NMNH -), which has a stability of -134.1 kcal/mol (-560.9 kJ/mol). These data suggest that we will be able to design NCBs with higher stability than those currently used in CFB.