In a quest to find the relevance of lactate as an extracellular signaling molecule, we found that acetyl-CoA synthetase is known to promote pro-tumor phenotype in macrophages. Also, acetyl-CoA synthetase is known for the metabolic activities in bacterial cells that could be linked with the microbiotas in the niche of the tumor microenvironment. By employing molecular interaction studies, data indicated that lactate (Figure ) occupies the same substrate site as in the case of acetate (Figure ). The binding affinity and interactions at the active sites of acetyl-CoA synthetase are projected to be almost similar from lactate (Figure ) over acetate (figure ) in terms of residues such as TRP413, TRP414, GLN415, ARG515, ASN512, and ARG526.