To gain a comprehensive overview and understanding of the chemical class annotation results for the Celastraceae set, a visual comparison was made with the collective set of molecules present in LOTUS and DNP for the Celastraceae family. For this, a sunburst plot of the reported chemical space was generated in the same way done for the CANOPUS chemical classes (Figure ). This showed that the main chemical superclasses included: diterpenes, sesquiterpenes, triterpenes, triterpenoids quinone methides, and maytansinoids. Within each of these superclasses, the main classes correspond to dihydro-β-agarofurans, macrolide sesquiterpene alkaloids (macrolactones formed from a dihydro-β-agarofuran and a pyridinic dicarboxylic acid), abietanes, friedo-oleanes, celastroloids, and macrolides, respectively. The most represented scaffold corresponds to terpene-like structures, which agrees with the chemical classes proposed by CANOPUS directly from the MS 2 spectra. This is, as well, consistent with the diverse phytochemical studies that have depicted the general profile of specialized metabolites within the Celastraceae family The distribution of the most represented chemical classes in this botanical family was visualized in Figure . Since the PI mode data exhibited superior annotation yields, these annotations were employed to determine the coverage proportion for these chemical classes, utilizing structural annotations from the dataset (as shown in Figure ). The most reported compounds are agarofurans sesquiterpenoids, followed by friedelane and lupane triterpenoids. Besides the excellent coverage in terms of chemical classes, the coverage within the most representative individual classes is high.