It is also acknowledged that the experimental design shown in Fig. is certainly not the overall best design, but it should suggest strategies for future work, especially when planning high-throughput LC measurements. Specifically, attention should be paid to developing a strategy that improves the valid range for using the model from 27.3 % organic composition to something closer to the 41 % valid range of organic composition observed for the isocratic data. In addition, we did not optimize the selection of the reference organic composition (ref), electing to use a value of 0.3 for all solute/column combinations. It is clear that further optimization of this value would yield further improvements in the accuracy of the parameter and retention factor predictions. Further consideration of the thresholds t1 and t2 that determine the initial classification of the solutes as being weakly, moderately or strongly retained should also be carried out.