aureus and E. coli were reactivated in brain BHI media at 37 °C for 48 h. After this period, samples of each activated microorganism were spread uniformly on the surface of plates containing Miller-Hinton Agar (Kasvi). Subsequently, the extract was placed on the surface of the plate with the aid of sterile forceps at equidistant positions. The same procedure was conducted with a control disk, Chloramphenicol (CLO-SENSIDISC 30). The plates were incubated at 36 °C for 24 h. Antimicrobial activity was determined by the formation of an inhibition halo as measured in millimeters.