Calculate the amount of reagents required for complete synthesis. The MerMade 12 calculates the amount of reagents needed by the injection volumes set in the protocol and the manual recommends adding at least an additional 20% to that estimate. We have found that using a modified ABI calculation of 50 couplings per gram (1g diluted to 12 mL) of phosphoramidites provides a better estimate to avoid running out of reagent. The recipes in the reagents section outline the procedure for producing approximately 10 mL of each reagent, adjust as necessary for your synthesis. Prepare reagents 5 through 8 as outlined in the reagents section. Add reagents to the synthesizer in the appropriate positions and prime all relevant lines. Upon completion of the synthesis, either proceed immediately to the deprotection steps, or store column in a dry place such as a desiccator cabinet.