Figure and b demonstrate how, at temperatures over 50ºC, the average micelle diameter decreased by more than 5 times. It ensures a rise in the amount of free DT in the extract as a result of micelle disintegration . Furthermore, it was noticed that applying a high temperature reduces the micelle structures irreversibly and decreasing the temperature to room temperature i.e. 25°C again thereafter did not change the shape of the DT-loaded micelle. As a result, it was discovered that temperature-induced harm to DT-loaded micelles was permanent.