The seeding setup for evaluation of the bilayer transition temperature was prepared in the following way. Two membranes consisting of 200 lipids were created using COBY 116 (Coarse-grained System Builder). The two systems were then minimized and equilibrated for 50 ns at 30 K above or below the known experimental transition of the given lipid type, to obtain the APL of the liquid and gel phases, respectively. Utilizing the found APL, COBY was then used to create a membrane consisting of 200 lipids in a liquid phase surrounding a gel patch also consisting of 200 lipids (the gel patch was inserted into the liquid bilayer). The system was minimized and equilibrated for 10 ns with separate temperature baths for the gel patch, simulated at 30 K below the experimental transition, while the surrounding liquid membrane was simulated at 30 K above the experimental transition temperature. Three independent repeats of the equilibration were performed with resampled velocities, resulting in three independent starting structures for the seeds simulations. The seed systems were then each simulated for 500 ns using a constant temperature bath for the entire simulation box, at temperatures ranging from 15 K below the experimental transition temperature to 15 K above, with steps of 2.5 K. The c-rescale barostat (τp = 4 ps) was used for equilibrations of the initial membranes where APLs and the gel patch were obtained as well as the final temperature range simulations. The Berendsen 187 barostat (τp = 4 ps) was used for equilibrations of the phase-separated systems since c-rescale cannot be used with separate temperature baths. The v-rescale 198 thermostat (τt = 1 ps) was used for all simulations. Simulations were performed using GROMACS v2024.0. The Lindemann Index 112 was calculated using a block of the last 100 ns of each simulation and averaged across the three repeats.