The DNA was transformed into BL21-DE3 cells and grown overnight at 37°C on an LB culture plate containing 100 µg/mL ampicillin. One colony was selected and placed into 10 mL SOC media containing 100 µg/mL ampicillin and grown overnight. The following morning, 1 L LB media containing 100 µg/mL ampicillin was inoculated with the 10 mL overnight growth and shaken at 170 rpm at 37°C until the OD600 reached 0.6-0.8 Abs. After reaching the optimal OD, the 1 L growth was induced by 0.1 mM isopropyl β-D-thiogalactoside (IPTG) and shaken at 170 rpm at room temperature overnight. The cells were harvested by centrifugation at 12000g for 30 minutes at 4°C and stored at -80 °C.