Targeted analysis is a top-down approach where all the necessary information for the unequivocal identification and potentially quantification of a chemical in a sample is available to the analyst prior to the analysis. Targeted analysis is the main strategy for routine monitoring of chemicals in environmental and biological samples. On the other hand, suspect and non-target analysis are less certain and also tend to be only qualitative, even though there have been several new developments in semi-quantification of known and unknown chemicals. For suspect analysis/screening, a list of suspect analytes with as much information as possible (e.g. predicted retention behavior, fragmentation spectra) is compiled, implying that suspect analysis, similar to the target analysis, is a top-down approach.