Depending upon solubility of drugs in organic solvents, polymer and drug stock solutions were mixed in desired ratios. After complete removal of the solvent at 50°C under a mild stream of argon, the films were further dried in vacuo (≤ 0.2 mbar) for at least 30 minutes to remove the traces of organic solvent (if any). Subsequently, preheated (37°C) DI water was added to obtain desired final polymer (10 g/L) and drug concentrations. To ensure complete solubilisation, the solutions were shaken at 55°C for 15 to 30 min at 1250 rpm with a Thermomixer comfort (Eppendorf AG, Hamburg, Germany). Non-solubilized drug was removed by centrifugation for 5 min at 10.000 rpm with a 3-Speed micro centrifuge, (neoLab, Heidelberg, Germany). Solubilisation experiments were performed in 3 individually prepared samples and results are presented as mean ± standard deviation (SD).