Similar experiments with (HA-g-Le x )-B and (HA-g-Gb3)-B, and their non-biotinylated precursors, demonstrated specific, saturable and stable anchorage of all these mucinlike structures (Figures ). In addition, (Ni 2+ -NTA)3presenting lipids were incorporated into a bilayer (Figure ) to capture the hexa-histidine anchor tag at the reducing end of (HA-g-Gb3)-H6. The his-tagged glycopolymer could be anchored specifically and stably via its H6 tag to the (Ni 2+ -NTA)3-presenting SLBs (Figure ). This illustrates the versatility of our approach to make model glycocalyces.