The PLS model for the FTIR measurements to follow the dynamic runs was developed with the previously described methodology. The RMSECV for the compounds 6, 2a, TBD, and 7 were 24 mM, 33 mM, 11 mM, and 28mM, respectively. The kinetics for the amidation step were fitted with the same reaction mechanism as described above (Figure ). The following kinetic parameters for the first step (intermediate formation) were fitted: A1 = 7.524 L/mol and Ea1 = 14.19 kJ/mol. In the second step (formation of product), the best-fitting parameters were: A2 = 6.659 L/mol and Ea2 = 11.5 kJ/mol.