For the elastic network, the four chains of the tetramer were merged, and we used a force constant of 700 kJ mol -1 nm -2 with an elastic bond upper cutoff of 0.8 nm. The termini were set neutral and side chain corrections (scFix option) were applied. Using insane , the protein was embedded in a lipid bilayer consisting of 100% POPC lipids in the upper leaflet, and 95% POPC and 5% PI(4,5)P2 (POP6) lipids in the lower leaflet; water and 0.15M NaCl were also added.