Deposition cycles the antibody increases, the sensor resistance increased accordingly and hit a plateau at 200 mM. Therefore, the sensor was fabricated with the optimal 200 mM Anti-L. Then, the incubation time of the lactate samples were optimized. The sensor response relied on the binding of the antigen and antibody. Sufficient time was required the binding to approach the equilibrium and output the maximum electrical responses. As shown in Fig. , the incubation time was increased to improve the electrical signal. Evidently, the sensors showed a maximum response at 60 min with nearly 45% increase in the channel resistance. Further increase in the incubation time would not help enhance the signals as the sensor surface was possibly saturated with the lactate binding. As a result, the sensor was operated with a 60min incubation of the samples.