Additionally, we used CrustalW, a bioinformatics computer tool for multiple sequence alignment, to carry out the sequence alignment . The sequences were organized using sequence alignment to identify common portions across aptamer sequences that might suggest structural and functional similarity. A phylogenetic tree is called a branching diagram or "tree" that illustrates the connections between the various aptamer sequences. Aptamer sequences are aligned during this process to arrange similar or identical nucleotides in the same column. From SELEX, 39 aptamers were selected for further evaluation and sequence alignment was divided into a set of two. Therefore, we have chosen only two of these two sets' aptamers. A phylogenetic tree analysis was used to confirm this further, as shown in Figure .