The CBP purified 800-mer was also subjected to the PCR, cloning and Sanger sequencing process. The image of the gel for electrophoresis analysis of the PCR product is shown in Figure . Even though the quantity of oligos was much lower, the band corresponding to the 800-mer is clear. The image of the gel for analysis of colony PCR products is shown in Figures . As can be seen, all colonies selected for the analysis had the 800-mer sequence. Plasmids of 47 colonies were subjected to Sanger sequencing. The data is provided in the supporting information. The results are summarized in Table . Among the 47 colonies sequenced, 45 contained the correct sequence, which was 96%. The errors in the incorrect sequences only include one deletion and one 2-nt deletion. The rates for both errors were 0.0027% . The sum of the error rates was 0.0054% (Entry 13).