To further extend the scope of SCMS, a novel platform integrating a commercially available cell manipulation system with the Single-probe technique was developed, allowing for the analysis of suspended cells such as leukemia cells . This Integrated Cell Manipulation Platform (ICMP) coupled with a high-resolution mass spectrometer was further used for quantitative analysis of intracellular metabolites from patient-derived suspension cells such as those in urine from bladder cancer patients (as detailed in Section 4.2.2) 34 . This system not only expanded the range of cell types that could be analyzed with minimal sample preparation but also enhanced specificity and sensitivity in distinguishing cellular features. The versatility of this approach highlighted its potential for personalized medicine, offering a rapid, real-time method to analyze live patient cells and tailor therapeutic strategies.