The data collected from the single lamella yielded a processed dataset that was 98.9% complete and direct methods were used to solve the structure of the FIB milled ZIF-8 sample. After several rounds of structure refinement, the final structure closely resembles previous ZIF-8 structures determined by X-ray crystallography and electron diffraction . The crystal was in space group I-43m with each MeIM molecule interacting with two zinc atoms through the ligand's nitrogen atoms. Each zinc is coordinated by a total of 4 different MeIM ligands to form the zeolite structure (Fig ). In the structure, volume of the solvent accessible voids is 2,323 Å 3 (48.7% of unit cell volume). This highresolution MicroED structure of ZIF-8 further demonstrates that cryo-FIB milling can preserve high resolution features even when working with sensitive samples. Previous cryo-FIB milling work on MOFs using high-resolution TEM showed that images in the range of 1.2 to 2.0 Å could still be obtained . This study demonstrates that quality structural information to at least 0.59 Å is still preserved after cryo-FIB milling of beamsensitive MOF crystals.