In order to detect the impact of elevated temperature on the exfoliation process, the reaction mixture was incubated at 50° C for 1 week. The increased temperature was expected to increase the reaction rate without impacting the yeast cells' growth. The obtained exfoliated structure was examined using spectroscopic techniques to determine its impact on the exfoliation process. The UV-Vis absorption spectrum and the Raman spectrum indicated no significant changes in the exfoliation yield and lattice properties of the obtained exfoliated structure for incubation at higher temperature.