The µF/mnESI setup demonstrated stable operation across the 31 h of acquisition of the 89-injection sequence. Pressure profiles were not altered across the sequence with an average backpressure of 860 bar. Retention times shifts did not exceed ±0.3 min from the average value (Fig. ). PCA score plot on raw data showed similar drift patterns between pooled QCs and re-conditioning QCs injected after diluted QCs, suggesting minimal of injection-to-injection changes in the matrix (Fig. ). In terms of repeatability, the μF/mnESI method displayed excellent performance compared to conventional AF/ESI methods, as exemplified with our previous AF/ESI acquisition of the same samples. Shares of lipids with CVQC < 10% were slightly higher for the µF/mnESI than for the AF/ESI setup: 60.0% (n = 762) vs. 48.0% (n = 361) for μF/mnESI and AF/ESI respectively (Fig. ). In addition, CVs were similarly spread across the elution gradient with both setups (Fig. ) and distributions of the measured analytical signals for both methods overlapped as bell-shaped curves with a slight shift towards higher abundances for the new setup (Fig. ). PCA score plots obtained from the conventional and the µF/mnESI datasets showed similar data structures before and after LOESS and PQN corrections (Fig. ), while the miniaturized setup displayed an increased drift across the sequence prior to correction (Fig. ). Outlier samples in the AF/ESI method were also observed among the most outlier samples in the µF/mnESI dataset (Fig. ).