Figure shows mean conformer free energy difference ∆F N (defined in the Methods section) obtained from clustering datasets of increasing N and a reference dataset at N =50,000 configurations. Figure shows a similar plot displaying the mean separation of the cluster centers, ∆d. Figure shows the number of minima identified by DPA for each reduced-size dataset. The plot shows that all reduced datasets agreed that there were 3 conformers, with the exception of the 10,000 configuration dataset. In all other datasets, there is very good agreement on the position and free energies of the local minima, with energy differences well within 1 kJ mol -1 and mean separations hovering around 0.1 rad.