Molecular docking was performed to using the Autodock vina program. During docking first AutoDock Vina, PyRx the target macromolecule is selected and it made in automatically pdbqt format when the protein made macromolecule. Then, the ligand is imported one by one, and then selected pdbqt protein and then click on start, the ligand automatically showed and selected it, also selected protein molecule. Then the docking is running and the result is produced. The negative values for binding affinity (or binding free energy) indicate that the ligand is predicted to bind to a target macromolecule; the more negative the numerical values for the binding affinity, the better is the predicted binding between a ligand and a macromolecule.