In the final experimental study we further investigated the possibilities in reaction miniaturization enabled by the use of NMP as non-volatile solvent. We found that with as little as one microliter of total reaction volume conversion to the anticipated products with reasonable reproducibility was obtained throughout the selected substrates (Figure ). To the best of our knowledge, this presents the smallest scale C-H activation reported to date, and presents an exciting opportunity in terms of improved sustainability of reaction screening by decreased material consumption. We were even able to further scale down the reaction by using acoustic dispensing for setting up the reaction plates. With this technique, we were able to detect product formations in reaction with a total volume as little as 5 nL (1 nmol scale). Taking Sulfaphenazole as an example, this means that from one gram of material 3181 reactions can be performed. Chemistry at this small scale brings its own set of challenges, and at this point we were not able to quantify conversions with reasonable accuracy and throughput. We are currently working on an AMI-MS analytical method to tackle this issue.