After treatment, the main internal organs and tumor tissues were removed for hematoxylin and eosin (H&E) staining to observe cell morphology, Terminal dUTP Nick End Labeling (TUNEL) staining to evaluate cell apoptosis and collagen deposition, CD8 + T staining to determine whether inhibiting immunosuppressive cells leads to enhanced T cell response and interleukin-2 (IL-2) staining to check the activities of the tumor-associated immune microenvironment, interferon-γ (IFN-γ) staining to verify anti-tumor and immunoregulatory effects, calreticulin (CRT) staining to evaluate the degree of immunogenic death of tumor cells.