However, the precision in determining the relative size of individual liposomes by multipassing is excellent. Figure demonstrates the advantage of making multiple measurements of the same liposome to achieve sub-nanometer sizing precision. In this experiment, a single liposome was multipassed 149 times through the nanopipette orifice. The ±3 SE (~99.7% confidence interval) from the moving mean of rL shows the dramatic decrease in measurement error with increasing number of measurements. At 5 translocations, the error of the reported liposome radius is ±0.5 nm, while at 149 translocations, the radius error decreased to ±0.1 nm. A 4-fold increase in the number of translocations (n) results in a 2-fold decrease in the error of the reported size due to the √n relationship of SE. In addition to obtaining sub-nanometer size resolution with multipassing, Figure also indicates that the liposomes do not change size as they are multipassed through the nanopipette orifice. An example of multipassing a fluorescent PS nanoparticle ~5500 times, resulting in a SE of ±0.01 nm, is shown in the Supporting Information (Figure ). ). The liposomes were also sized with dynamic light scattering (DLS) (Figure ). A comparison of the sizing methods is shown in Figure and show agreement between the two methods, rcryoTEM = 39 ± 15 nm and rDLS = 45 ± 15 nm (here, the reported error is the standard deviation of the distribution of the radii). As expected, the average radii determined with DLS is slightly larger than the radii determined with cryoTEM imaging due to the weak weighting of smaller particles in the DLS measurements.