The mammalian brain has a very limited capability of regeneration of lost neurons and regaining function after injury. Promoting the migration of young neurons derived from endogenous neural stem cells (neuroblasts) using biomaterials is a promising new approach to help the brain recover after injury. However, delivering sufficient neuroblasts to distant injury sites has been a critical challenge due to the limited number of scaffold cells to induce neuroblast migration. It has been shown that hydrogels consisting of A16G and A16G-tagged N-cadherin (A16G-Ncad) can address the above issue. 17 Ncadherin plays an important role in the migration of neuroblasts along radial glial fibres in the injured neonatal neocortex. 32 Time-lapse imaging of ventricular-subventricular zone neuroblasts on culture dishes coated with A16G or a mixture of A16G and A16G-Ncad hydrogels showed that the migration rate of neuroblasts was significantly higher on the hydrogel containing A16G-Ncad than on A16G alone (Fig. ). When the neonatal neocortex is injured at low temperature, and, subsequently, hydrogels composed of A16G, a mixture of A16G and A16G-Ncad or a mixture of A16G and Ncad are injected at the site of injury, Dcx+ (a marker for neuroblasts) is abundantly observed in the hydrogel of the mixture of A16G and A16G-Ncad. In contrast, neuroblasts are seen sparsely in the hydrogels made of A16G alone and a mixture of A16G and Ncad (Fig. ), indicating that A16G hydrogel containing A16G-Ncad efficiently facilitates the migration of neuroblasts into the injured neocortex in vivo. This study demonstrates that fibrous selfassembly of A16G peptide and A16G-Ncad mimics both the structure and dynamic function of intrinsic scaffold cells, providing a novel strategy for neuro-regenerative therapy.