After confirming hydrogel degradation at a microscopic level by analyzing fluorescent bead movements, the overall hydrogel degradation was evaluated by measuring the remained gel weight after stress application. To remove the degraded portion from a hydrogel after compression, the gel was rinsed on a wetted hydrophilic nylon filter membrane with a vacuum apparatus and the residual hydrogel weight was measured (Figure and Supporting Information). As expected, the hydrogels that contained EGTA-loaded vesicles showed significant weight reduction (90%) after the compression was applied (Figure ). The difference is clearly shown in the photographs in Figure . A lower stress of 33 kPa was also found to trigger hydrogel degradation at a slightly lower degree compared to 55 kPa (Figure ).