The photoprotection observed for OTPh and P3HT in the self-assembly with NaDC is relevant when developing materials that include conjugated polymers in an aqueous solution or in viscoelastic media, such as hydrogels. The photodegradation of conjugated polymers and oligomers results in the loss of fluorescence due to the reaction of these molecules with singlet oxygen that disrupts the π-conjugation of conjugated oligomers and polymers. Singlet oxygen is formed after the energy transfer from the tripled excited states of the polymers or oligomers to ground-state oxygen. The protection afforded by the NaDC aggregates compared to the reaction in chloroform is a combination of the lower oxygen concentration in water and the lower oxygen quenching rate constant when molecules are bound to bile salt aggregates compared to the quenching in water. The latter process is related to the incorporation of the oligomer or polymer in the self-assembly with NaDC. This self-assembly led to a better protection from photodegradation for OTPh than for P3HT, suggesting that some sites of the polymer are exposed to the aqueous phase. In addition, the protection from photodegradation was less efficient in the gel than in solution. This result suggests that for the gel the accessibility of oxygen to the excited states of OTPh and P3HT, or the lifetimes of these excited states are different from the excited state behavior of OTPh and P3HT in the NaDC aggregates in solution. One possibility is that the dynamics of the NaDC primary and secondary aggregates is slower when the self-assembly is immobilized in the gel. Faster dynamics of the NaDC aggregates could increase the average coverage of OTPh and P3HT in solution which would decrease the overall accessibility of oxygen to the reactive sites of OTPh and P3HT.