Cholesterol-protein interactions are fundamental both for the correct organization of biological membranes, as well as for cellular homeostasis. With this in mind, our model must correctly replicate known interaction behavior. To test this, we probed the recognition and binding of our cholesterol model by 3 cholesterol-binding proteins: the human β2-adrenergic receptor (β2AR), the transmembrane domain of the hedgehog signal transducer and class F G protein-coupled receptor (GPCR) smoothened (SMO), and the voltage-dependent anion channel 1 (VDAC1). These 3 selected proteins showcase well the typical cholesterol-protein interactions which are usually explored using Martini and include extensive reference data for validation .