The approximate hydrodynamic size distributions and dispersity indices of nano assemblies formed by MHF were determined through (multi-angle) dynamic light scattering measurements. Collected samples were placed directly into a plastic cuvette and diluted 10:1 in 1 x PBS at pH 7.4, and a size distribution obtained using Malvern Zetasizer Ultra software, using refractive index values for neat monoolein and phytantriol (1.47). Software-generated correlation graphs and laser auto-adjustment values were used to measure the reliability of gaussian distributions obtained (correlogram intercepts should > 0.9).