The initial screening of MIPE4 library was performed in a duplicated single-agent dose-response manner (11 serial dilutions 1:3 in the range of [46uM -0.78nM]. For this assay, PANC-1 cells were grown in DMEM media with 10% FBS and 1% antibiotics mix. The day of the experiment, they were harvested with 0.25% Trypsin for 5min, spun down and seeded as a cell suspension in growth media 500 cells/5ul/well. The cell density was experimentally selected based on efficient proliferation rate of cells for the period of 72 hours. Cells were plated onto white tissue culturetreated 1536-well Aurora plates (Brooks Automation, Chelmsford, MA) with MultiDrop Combi dispenser (Thermo Scientific, Logan, UT), and allowed to attach overnight at 37°C, 5% CO2. Next, 23nl/well of compound solutions in DMSO were added to cells with a pintool transfer (Kalypsis, San Diego, CA). As the positive control, we used "no cells" column which would be equal to 100% killing capacity of a compound (IC100), and DMSO was used as a vehicle neutral control (IC0).