The pyramid of stress tolerance genes has emerged as a powerful strategy for creating crops with broad environmental adaptability. By combining multiple stress resistance mechanisms, researchers can develop plants capable of withstanding various abiotic and biotic stresses simultaneously . In the realm of nutritional enhancement, stacking genes for various nutritional traits has led to the creation of more comprehensive biofortified crops, addressing multiple nutrient deficiencies in a single plant variety . Moreover, metabolic balancing through the fine-tuning of multiple pathway components simultaneously has proven crucial in optimizing flux and avoiding metabolic bottlenecks in engineered pathways . The development of advanced cloning techniques, such as Golden Gate assembly and Gibson Assembly, has greatly facilitated the creation of multigene constructs for trait stacking . As our understanding of plant metabolism and gene interactions deepens, multi-gene trait stacking is expected to play an increasingly important role in developing the next generation of improved crops.