If a cosolvent prefers to interact with a solute, it will replace the water molecules from the surroundings of the solute. So, a positive value of г indicates a pronounced accumulation of cosolvent around the solute whereas a negative γ value indicates that a preferential exclusion of cosolvent from the surroundings of the solute. However, the effect of cosolvent is negligible when |γ | < 1. In our current discussion the solute is neopentane and the cosolvent is either urea or TMAO. Around the neopentane molecule, the preferential binding coefficient for urea and for TMAO in water, waterurea, water-TMAO and water-urea-TMAO mixtures are computed using Eq. 2 and the data is shown in Table . It can be seen that the γ values for urea are always negative. Using this finding, it can be concluded that urea is preferentially excluded to neopentane in water-urea mixture or in water-urea-TMAO mixture. From this data, we can see |γ | < 1 for TMAO in water-TMAO or in water-urea-TMAO mixture, no matter which force fields are used for TMAO. Hence, TMAO does not provide any significant change in water binding to neopentane. A positive (>1) value of γ for TMAO around hydrophobic polymer is always observed by Mondal et al. both in collapsed and extended form of the polymer. The number of interacting sites are more for the polymer molecule in comparison to that in small molecule like neopentane that may be one possible reason. The interaction parameters (σ,ε) on each site of the hydrophobe is also equally important.