SAXS appears more sensitive to the electron density at the centre of the PEO hydrophilic corona and can collect data from the entire sample, approximately ranging from 10 10 -10 13 vesicles at once. However, the scattering pattern can be problematic to model without a priori structural information. Cryo-ET and SPA images provide high contrast images with a large gradient in electron intensity at the hydrophilic-hydrophobic interface of the membranes, however the results are always specific with respect to the analysed vesicles rather than representative of the whole sample.