Despite these limitations, we can still compare the available findings. In the previous study, 5 of the 150 ML-nominated compounds were confirmed as hits in dose-response titrations via SPR, resulting in a hit rate of approximately 3%, with KD values ranging from 6 to 250 μM. When comparing the ML-nominated compounds from that study with those from our current work, none had Tanimoto similarity scores above 0.4. Notably, 5 compounds from that study contained both a cyano and an amide group, but none were identified as binders in SPR screening at a single compound concentration of 45 µM. In contrast, the current study yielded a 15% hit rate, with KD values ranging from 2.7 to 20 µM.