In this article, we report the results of a study of the electrocatalytic activity of decomposition of hydrogen peroxide on electrodes formed by MnO2 electroplating at various pH from sulphate electrolytes on the surface of indium tin oxide coated glass (ITO) using several electrochemical techniques. The composition, morphology, and conductivity of the formed films were studied. Subsequently, the electrodes were modified with the enzyme glucose oxidase (GOx) and tested for their performance in determining glucose at a given concentration. The study was carried out with the aim of forming sensitive electrodes in biosensors for the determination of glucose and, by extension, other analytes based on the determination of H2O2 from oxidase-catalysed reactions.