0.05 M of acetate buffer (pH 3.8) was prepared with sodium acetate (0.6 g) and acetic acid (2.6 g) in 1 L of ultrapure water. For the preparation of the continuous phase, egg yolk was mixed in the buffer with a concentration of 1 wt.%. Stripped soybean oil was prepared using alumina powder to remove impurities and lipid-soluble antioxidants . Briefly, the oil was mixed with the powder at a volume ratio of 2:1 in Falcon tubes and shaken for 24 hrs. The suspension was then centrifuged at 2000×g for 20 min. The oil was collected, and the same centrifugation procedure was repeated to ensure the complete removal of the alumina powder. For providing a marker of lipid oxidation, 50 µM of BODIPY 665/676 was pre-mixed in the stripped soybean oil before making emulsions. A coarse emulsion was first prepared by adding 10 wt.% of stripped soybean oil to the continuous phase, and high-speed stirring was applied at 11,000 rpm for 1 min with a rotor-stator homogenizer (Ultra-turrax IKA T18 basic, Germany). Next, a fine emulsion was prepared by passing the coarse emulsion through either, (i), a lab-scale colloid mill with a gap width of 0.32 mm (IKA Magic Lab, Staufen, Germany), operating for 1 min at 26,000 rpm or, (ii), a microfluidizer (Microfluidics, Massachusetts, USA), equipped with a Y-shaped interaction chamber (F12Y; minimum internal dimension: 75 μm), operating at 600 bars during three passes. During the operation, the colloid mill was cooled with water at 4 °C, and for the microfluidizer, the sample chamber was placed in an ice bath. Oxidization was induced by incubating 1 mL of emulsions at 40 °C with 5 mM of the radical initiator (AAPH) in the dark over two days.