Effect sizes were divided into porous and non-porous substrates (Table ). 71% of time-series experiments were conducted using porous substrates (Fig. ) and the effect sizes differed from nonporous substrates (F = 22.37, p-value <0.001), with the latter demonstrating a greater mean effect size (Fig. ). Blood sources were divided into six subgroups; 84% of statistics were computed from studies using human blood (Table , Fig. ) and no difference was found between subgroups (F = 1.15, p-value = 0.34). Temperature was divided into 4 subgroups (Table , Fig ) and no differences were observed (F = 0.45, p-value = 0.72). Nearly 90% of experiments were conducted at room temperature (18 -25°C). Timepoints sampled in the retained studies varied, with most studies analyzing blood after one and two weeks of deposition (Fig ). Studies that analyzed genetic material typically had longer time series than protein-based approaches (Fig S1 ).