Beyond the Martini models themselves, there are important considerations regarding the protocols we suggest. The degree of protonation of ILs at different pH levels was not explored in this work. We approximated IL lipids with pH 7 or higher as fully neutral and lower pH (5-6) as fully protonated. Better consideration of the expected degree of protonation based on apparent pKas, the influence of the environment (especially nucleic acid cargo), or the use of constant pH approaches could improve the assignment of protonation states. Further development of Martini constant pH approaches, such as the Titratable variant , would also be beneficial.