We have shown that pBAE-CRHR-Zw displays good packaging capacity and low transfection. To conditionally recover their transfection potential with spatiotemporal resolution, we engineered a linker peptide that could be cleaved enabling the release the zwitterionic moiety upon light irradiation. Accordingly, we synthesized a peptide containing a nitrobenzyl (NB) group and incorporated it in between the CRHR and the zwitterionic peptide (pBAE-CRHR-NB-Zw, Figure ), similarly as we have recently reported to generate light-sensitive antibody-polymer conjugates . The nitrobenzyl linker peptide can be cleaved under UVA light irradiation at 365 nm . Despite its relatively low tissue penetration, UVA light is used in clinical settings, thus the photo-cleavable NB linker could enable therapeutic application.