The CG model implemented in cg_pyrosetta consists of a series of connected CG beads, which can represent whole or parts of the side chain and backbone moieties. This representation was chosen as a starting point as it preserves the notion of individual residues and distinguishes between backbone and side chain interactions. This construction also allows us to build more complex models by adding more interaction sites or interaction potentials to either the backbone or the side chain sites. Many commonly-used protein CG models reduce full-atom representation of a protein to a similar representation while maintaining sufficient information to study protein folding, protein docking and large-scale protein dynamics. Our CG model implemented in cg_pyrosetta uses the standard Rosetta score functions to implement Lennard-Jones pairwise potentials and the Rosetta molecular mechanics score functions to implement harmonic bond angle potentials, periodic dihedral angle potentials. Electrostatic potentials were not used in this work, but can be explored as needed, using either new or existing Rosetta score terms. Potentials used in cg_pyrosetta and their corresponding score terms can be found in Table . In this work, we assume bond lengths are rigid at their equilibrium value. This assumption was made in part to reduce the dimensionality of the configuration-space we are sampling and in part due to the minimal contribution bond stretching contributes to overall protein motion. Table : Energetic terms, relevant parameters and potential equations and their corresponding score terms in Rosetta. Pairwise mixing in Rosetta is handled such that, i,j is the geometric mean of i and j and R min i,j is the sum of R min