Several case studies have demonstrated the successful application of metabolomics in investigating plant-endophyte interactions. For example, a study by used LC-MS to investigate the metabolic changes in tomato plants inoculated with the endophytic fungus Trichoderma harzianum . The authors identified several metabolites, such as flavonoids and steroidal glycoalkaloids, that were differentially accumulated in the inoculated plants and could potentially contribute to their enhanced growth and stress tolerance. Similarly, used LC-MS and GC-MS to investigate the metabolic changes in tomato plants inoculated with the endophytic bacterium Pseudomonas putida KT2440. The authors identified several metabolites, including amino acids, organic acids, and fatty acids, that were significantly altered in the inoculated plants and could contribute to their improved growth, stress tolerance, and disease resistance . These case studies highlight the power of metabolomics in unraveling the complex metabolic interactions between plants and their endophytic partners and provide valuable insights for harnessing these associations for sustainable agriculture and biotechnology.