Fig. ZipChip CE-MS Limit of Detection testing for AAV capsid analysis. A) Total ion electropherograms of AAV8E VPs analysed at decreasing concentrations. The peaks representing the VP proteins are encased in the grey box. The other peaks detected are those of host cell contaminants. The VP peak area shown is an average of the total VP peak area of the 5 injections run at each concentration of capsids injected. B) MS spectra of the total area of the VP peaks in the electropherograms described in A). Spectral intensity value shown is the average spectral intensity of area for the 5 injections run at each concentration of capsids injected. Injection 3 of each sample concentration analysed is used as a representative injection for the electropherograms and the MS spectra shown in A) and B), respectively.