discovery. We also report robust, ensemble-based observation of conformational changes that occur at the main binding site of 3CLPro due to the presence of another ligand at an allosteric binding site explaining the underlying cascade of events responsible for its inhibitory effect. Using our simulations, we have discovered a novel allosteric mechanism of inhibition for a ligand known to bind only at the substrate binding site. Due to the chaotic nature of molecular dynamics trajectories, individual trajectories do not allow for accurate or reproducible elucidation of macroscopic expectation values. Unprecedentedly at this timescale, we compare the statistical distribution of protein-ligand contact frequencies for these ten/twelve 10-microsecond trajectories and find that over 90% of trajectories have significantly different contact frequency distributions. Furthermore, using a direct binding free energy calculation protocol, we determine the ligand binding free energies for each of the identified sites using long-timescale simulations. The free energies differ by 0.77 to 7.26 kcal/mol across individual trajectories depending on the binding site and the system. We show that although this is the standard way such quantities are currently reported at long-timescale, individual simulations do not yield reliable free energies. Ensembles of independent trajectories are necessary to overcome the aleatoric uncertainty in order to obtain statistically meaningful and reproducible results. Our findings here are generally applicable to all molecular dynamics based applications and not confined to the free energy methods used in this study. Finally, we compare the application of different free energy methods to these systems and discuss their advantages and disadvantages.