Finally, we emphasize that our simulations focused on the location of the imidazole bridged dinucleotide intermediate near the 5 ′ -end of the template. In fact, the more labile and flexible activated dinucleotides could exhibit somewhat higher binding specificity when placed in the middle of the template and accompanied by a downstream helper oligonucleotide . This selection of the model was largely dictated by the initial usage of the crystal structure for our parent GpAIpG system, which was originally acquired by Zhang et al. . However, despite this possible limitation of the model, it complements the previous results of Mittal et al. by showing the molecular intricacies of the process at the end of the template, which were not addressed up to now. Importantly, it demonstrates the fragility of the processes, emphasizing that some oligonucleotide sequences cannot be easily copied in full, particularly if the templating strand does not contain two C or G nucleotides at its termini. This could result in the possible prebiotic pre-selection of sequences which posses a higher potential to be copied in full, to prevent fractionation into a diverse pool of too short and non-functional oligomers.