The differences in fluorescence imaging during early germination stage in both water and alkaline environments can act as an early marker for assessing the growth potential of pea seeds under stress. Combining these findings with the previous results, the semi-empirical model achieved an accuracy of 100% for predicating harvest, 93.8% for germination percentage and 87.5% for vigour index. These semi-empirical findings are essential for selecting appropriate varieties for cultivation across various soil environments and for determining optimal planting conditions for new varieties.