In the initial screening, plates were precoated with troponin T or BSA (negative control) by incubating 100 μl of 1 μg/ml appropriate protein solution. Then plates were blocked with 0.1% BSA for 2 hours at 4℃ and washed 4 times with 0.1% PBST. Every isolated phage clone solution (phages concentration 10 10 pfu/ml, 100 μl) was incubated with TnT and BSA for 1 hour at 350 rpm at room temperature. Then wells were washed 4 times using 0.1% PBST, 100 μl of HRP-antibody conjugate was added, and plates were incubated as previously. After 1 hour, plates were washed as before, and 50 μl of TMB was added. After 5 minutes, the reaction was stopped by adding 50 μl of 2 M sulfuric acid. Absorbance at 450 nm was read using Synergy HTX Multi-Mode Reader (BioTek) plate reader.