Today, plant metabolomes can be efficiently characterized through untargeted Ultra-High-Performance Liquid Chromatography-High-Resolution data-dependent tandem Mass Spectrometry (UHPLC-dd-HRMS ). This provides HRMS and corresponding MS 2 spectra on most detected ions . The aligned MS feature tables from UHPLC-HRMS 2 facilitate the detection of variations in the metabolite composition between samples, while annotation/dereplication strategies provide an in-depth overview of the chemistry linked to each organism .