Finally, not all in silico tools or platforms use the same method of substrate prediction, and devastatingly, many of them have not been maintained over the years. It is our hope that the natural product research community will endeavor to uphold a wide variety of bioinformatic platforms, which will allow for more accurate predictions in the future, and the subsequent isolation of new bioactive molecules. Two somewhat recent comprehensive reviews of various platforms 135 and alignment-free methods 136 are available, and we present in the sections below an updated and abbreviated NRPS-focused comparison.