ZIF-8 emerges as a promising choice for mRNA delivery, given its extensive exploration in biomedical and drug delivery studies. The unique ability of ZIF-8 to mineralize under gentle aqueous conditions, a process known as biomimetic mineralization which has been utilized to encapsulate biomolecules and cells, and degrade at acidic pH makes it an ideal candidate for mRNA delivery, compared to other MOFs. Recognizing the delicate nature of mRNA, our investigation focused on identifying synthetic conditions for ZIF-8 encapsulation that preserve mRNA stability, considering mRNA length and expressibility in vitro. Initially, we assessed mRNA length using a native agarose gel, and expressibility was evaluated with treated mRNA and Lipofectamine Messenger Max (Lipo MM) in HEK293t cells.