The setup consisted of a 4 mW HeNe laser with a wavelength of 633 nm and an Avalanche photodiode located at a fixed scattering angle of 90 • relative to the incident laser beam. We note that performing scattering at this relatively large wave-vector may introduce some wave-vector dependence on the hydrodynamic radius; however, our results indicate that these measurements are consistent with intrinsic viscosity and computer simulations and thus we do not pursue studying this wave-vector dependence. Bottlebrush samples were diluted in chlorobenzene to concentrations ranging from 4 mg/mL to 12 mg/mL and loaded into glass cuvettes. Prior to testing, the samples were heated to a temperature of 30 • C at which point they were allowed to reach thermal equilibrium over a period of 5 min. During each test, the intensity of light as a function of time was recorded and compared with itself to generate an autocorrelation function. Using the Zetasizer software package provided by Malvern, a set of exponential decay functions were fit to the autocorrelation function according to the cumulants method. From the center-of-mass diffusivity was determined from the exponential decay coefficient, which was in turn used to calculate the hydrodynamic radius according to the Stokes-Einstein equation. A more detailed description of this process is provided as Supporting Information.