EGFR and PTEN are abundant in clathrin-coated pits (CCP) when coupled to EGF . PTEN regulates clathrin-coated pits (CCP) dynamically . The formation of cytoplasmic-coated vesicles by clathrin-coated pits (CCPs) in the plasma membrane may take 20 seconds to 1 minute . Viruses exploit both clathrin-mediated endocytosis and macropinocytosis as cellular routes to induce productive infection . Hantavirus enters the host cell via clathrin-dependent endocytosis after it comes into contact with the cell surface . Hantavirus particle is internalized when it attaches to cell receptors by clathrin-mediated, dynein-independent, or macropinocytosis-like pathways . After that, the particle is sent to the endosomal compartment . There is a possibility that the virus and endosomal membrane fuse there . Hantaviruses from the old world (HTNV, SEOV) are absorbed by clathrin-mediated endocytosis . Dynein, clathrin heavy chain, and adaptor protein AP2 (clathrin's three principal components) are the primary target of ANDV internalization and the dependent endocytic pathway . Multiple genes involved in cholesterol sensing, regulation, and biosynthesis are required for Hantaviruses from the old and new worlds . ANDV has been demonstrated to be dependent on host components unrelated to dyneins, such as cholesterol and the Rho GTPase Rac11 . Following membrane fusion, which requires cholesterol and an acidic pH, the viral nucleocapsid escapes into the cytoplasm and commences genome replication . It implies that the hantavirus membrane glycoprotein protein possesses PTEN activity, participating in endocytosis mediated by clathrin.