Colloidal solution of metals has been utilized for energy, water treatment, catalysis, sensor, biomedical, and many other applications. In most of these cases, a large amount of emphasis has been given to processes to increase composition/concentration of metallic nanoparticles in the colloidal. There is limited study on the process of dilution (1bpm or ultra-dilution beyond and its effect on different physical and chemical properties. Since 1796, a highly diluted colloidal solution is used for medication in Homeopathy. The various literature of Homeopathy medicines suggest that colloidal solutions are more effective with higher potentization/dilution (composed of serial dilutions and vigorous triturations/successions beyond Avogadro no-10 23 ). Multiple studies about homeopathic potencies and nanoparticles (NPs) have been published . The study published by Chikramane et al. , showing the presence of starting material in the form of nanoparticles, has been the most pivotal. Following this study, multiple advancements have been made further to understand the complex interactions of homeopathic medicine. Basu et al. reported the physico-chemical changes in nanoparticles due to Homeopathic trituration and succussions. Wassenhoven et al. performed nanoparticle characterization of homeopathic medicines and showed the nanoparticle-induced hormetic activation . Bhattacharyya et al. demonstrated the nanoparticles enhance cellular uptake and increase bioactivity. Many groups are still working to explain the origin of nanoparticles while processing. The change in size and morphology of the nanoparticles during different potencies is not explained. Also, the increase in the effectiveness of the medicine in higher potency is not clear.