The protein AVO28_00330 was predicted to contain 389 amino acids where Leu (51) and Trp (5) are most abundant and least abundant, respectively (Table ). The molecular weight was calculated as 44336.69 Da and theoretical pI was 5.94, indicating the protein to be acidic and negatively charged. Total number of positively charged residues (Arg + Lys) and total number of negatively charged residues (Asp + Glu) were found 46 and 54, respectively. The instability index was 41.32, indicating the unstable nature of the protein . Aliphatic index was 88.12 which gives an indication of proteins stability over a wide temperature range. The GRAVY was -0.366, which indicates the protein is nonpolar and hydrophilic. This also indicates better interaction possibility with water . High extinction coefficient value (47370) indicates the presence of Cys, Trp and Tyr residues . The N-terminal of the sequence was considered M (Met). Protein half-life is an estimation of the period of time which is required for the radiolabeled focus protein density to be decreased by 50 percent compared to the amount at the onset of the chase . Estimated half-life was found to be 30 h in mammalian reticulocytes (in vitro), >20 h in yeast (in vivo), >10 h in Escherichia coli (in vivo). Total number of atoms and molecular formula were 6189 and C1942H3082N562O579S24 , respectively.