<prompt> Answer this question truthfullyWhat laboratory results indicate delayed puberty?  <response>: <reason> First line tests in the diagnosis of delayed puberty are complete blood count, erythrocyte sedimentation rate, creatinine, electrolytes, bicarbonate, alkaline phosphatase, albumin, thyrotropin, free thyroxine, luteinizing hormone LH, follicle stimulating hormone FSH, insulin-like growth factor IGF-1, and testosterone. For specific familial disorders some special laboratory tests may be needed, such as anti- gliadin antibody and anti- tissue transglutaminase antibody i.e., Celiac disease diagnosis or anti-neutrophil cytoplasmic antibodies i.e., inflammatory bowel disease diagnosis. Second line tests are gonadotropin-releasing hormone GnRH, human chorionic gonadotropin hCG test, inhibin B, prolactin, and growth hormone GH test. 