 Amakhemikhali Ahlanganisiwe eLipid Nanoparticles nge-Ultrasonics - Hielscher Ultrasound TechnologyIzithwali zezidakamizwa ezinobukhulu obukhulu be-Nano zisetshenziselwa kabanzi ukuletha amakhompiyutha asebenzayo emithini kumaseli ahlosiwe. Ukuze ubethele izinto ezisebenzayo kwisithwali somuthi esine-bioavailability ephezulu, kusetshenziswa ama-nanoparticles aqinile we-lipid. I-Ultrasonic nano-emulsization kanye ne-encapsulation iyindlela ethembekile yokukhiqiza inani elikhulu lezinto ezisezingeni eliphakeme ze-nano ezifana ne-lipid nanoparticles, i-nano-ehleliwe ye-lipid carriers kanye ne-liposomes.Phakathi kwalesi sifo esiwubhubhane se-COVID-19 Hielscher Ultrasonics sibeka yonke imiyalo yomkhakha wezemithi. Sicela ubuze ukucutshungulwa kwe-oda kusengaphambili kanye nokuhanjiswa kwempahla okusheshayo!I-Ultrasonic Nano-Emulsification ne-EncapsulationI-Sonication iyakwazi ukuphazamisa izigaba zamafutha nezamanzi futhi ixube amaconsi amancane e-oyili emanzini. Njengoba inqubo ye-emulsification ye-ultrasonic ingalawulwa ngokuqondile, i-emulsization ye-ultrasonic kanye nenqubo elandelayo yokwakha i-nanoparticles eqinile-yolid iyakwazi ukukhiqiza i-sonoparticles sonication ekhiqizayo ingakhiqiza ubukhulu obukhulu be-Droplet ngokuqhathaniswa nezindlela ezijwayelekile ze-emulsion.Njengoba i-nano-emulsization ye-ultrasonic n encapsulation ingalawulwa ngokuqondile, inqubo ye-sonication ivumela ukulawulwa okuqondile kwesayizi eqinile ye-lipid nanoparticle nokulayishwa kwayo.I-Ultrasonic nano-emulsation isetshenziselwa ukukhiqiza ama-nano-emulsions, ama-liposomes nama-nanoparticles aqinile. I-Sonication ikhiqiza ukusatshalaliswa kwe-droplet emincane kanye nezinhlayiya ezihlelwe ngendlela ethile.I-Hielscher Ultrasonics homogenizer ikunikeza ukulawula okugcwele kwamapharamitha enqubo anjenge-amplitude, izinga lokushisa, ingcindezi, isikhathi se-sonication nokufakwa kwamandla. Lokhu kukuvumela ukuthi ukhulise iresiphi eyenziwe ngezifiso yokwakheka kokuqina kwe-lipid nanoparticle. Ukulungiswa kwe-lipid nanoparticles kuhambelana nemithombo eminingi ye-lipid nama-emulsifiers.I-Sonication iphumela ekuhanjisweni ngosayizi omncane wezinhlayiyana, ngakho-ke kuholela ekuzinzeni okuphezulu ngesikhathi sokulondolozwa.I-UIP1000hdT ngomphumela wokugeleza kwengilazi ukukhiqizwa okuqinile kwe-lipid nanoparticleUkulungiswa kwe-Ultrasonic kweSolid-Lipid NanoparticlesIzinyathelo zokukhiqizwa kwe-ultrasonic zeSolid-Lipid NanoparticlesAyini ama-Solid-Lipid Nanoparticles?Ama-solid lipid nanoparticles SLNs ayindlela ejwayelekile yokuthwala izidakamizwa e-nano, engakwazi ukufaka amakhompiyutha we-bioactive noma wemithi. Bangakwazi ukuthuthukisa ukulethwa kwezidakamizwa kuma-lymphatics wamathumbu futhi bathuthukise ukungena ngaphakathi kwezicubu ngezinga eliphakeme lokulethwa kwama-Therapeutics. Ama-SNL angafaka izidakamizwa ze-lipophilic emgodleni wazo we-lipid, kanti ukugoqa okwenziwe ngenhla kwenza i-nanoparticle inganyibiliki emanzini futhi ibanikeze i-bioavailability ephezulu. Ama-SNL anesimo esiyindilinga futhi aqukethe umsuka oqinile we-lipid oqiniswe i-ejenti yokuqinisa i-survivant, co-survivants.Imithombo ejwayelekile ye-lipid ne-triglyceride yi-tricaprin, trilaurin, trimyristin, i-tripalmitin, i-tristearin, i-glyceryl monstearate, i-glyceryl behenate, i-glyceryl Palmitstearate, i-cetyl palmitate, i-acid ye-stearic, i-palmitic acid, i-decanoic acid, i-behenic acid, i-glycol estes.Njengoba i-survivant kuma-lipid nanoparticles aqinile ama-lecithin isib. I-soy lecithin, i-lecithin yeqanda, i-lecithin yamaqanda, i-phospholipids, i-phosphatidylcholine, i-sphingomyelins, i-bile salts i-sodium taurocholate, ama-sterols cholesterol, i-poloxamer 188, 182, 18olo, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182, 182. , i-polysorbate 20, 60, ne-80, i-sodium cholate, i-sodium glycocholate, i-taurocholic acid sodium usawoti, i-taurodeoxycholic acid sodium usawoti, butanol, butyric acid, dioctyl sodium sulfosuccinate, monooctylphosphoric acid sodium phakathi kwabanye isetshenzisiwe.Njengoba ama-nanoparticles e-lipid aqinile aqukethe ama-lipids afanayo ngokomzimba atholakala emzimbeni womuntu, abhekwa njengabekezeleleke kahle. Zingahanjiswa ngomlomo, ngaphakathi nangaphakathi.Ama-Ultrasonicators aphezulu enza umsebenzi we-Solid Lipid NanoparticlesIzinhlelo ze-Hielscher Ultrasonics zisetshenziselwa kabanzi ekwenziweni kwemithi nokwengezekisa ukwakha ama-nanoparticles aphakeme aphezulu we-lipid nama-liposomes afakwe izinto zemithi, amavithamini, ama-antioxidants, ama-peptides nezinye izinto ezihlanganisiwe. Ukuhlangabezana nezidingo zamakhasimende ayo, abakwaHielscher bahlinzeka ngama-ultrasonicators avela ku-compact, nokho enamandla aphethwe ngesandla ama-lab homogeniser nama-bench-top ultrasonicators ezinhlelweni ze-ultrasonic zezimboni ngokugcwele zokukhiqizwa kwamanani aphezulu wezinqubo zokwenziwa kwemithi. Uhla olubanzi lwama-sonotrodes we-ultrasonic kanye nama-reactors ayatholakala ukuqinisekisa ukusethwa okuphezulu komkhiqizo wakho we-liposome. Ukuba namandla kwemishini yokusebenza kwe-Hielscher sika-Hielscher kuvumela ukusebenza kwe-247 emsebenzini osindayo nasezindaweni ezifunwayo.Basarkar A., Devineni D., Palaniappan R., Singh J. 2007: Ukulungiselela, ukubekwa uphawu, ukusebenza kwe-cytotoxicity kanye nokudluliselwa kokusebenza kahle kwe-poly D, L-lactide- co-glycolide ne-poly D, L-lactic acid i-cationic nanoparticles yokulethwa okulawulwayo kwe-DNA ye-plasmid. Int J Pharm 343: 247-2525.I-Dai Ch., Jin Z., Zhao Y., Cui H., Wang Y. 2013: Ukulungiselela kanye nokusebenza kwe-Newcastle Disease Virus DNA Vaccine Kuhlanganiswe ku-PLGA Nanoparticles. I-PLoS ONE 8 12, 2013.I-Solid Lipid Nanoparticles njenge-Drug CarriersImijovo ye-RNA 