<qa>  What will be used to determine changes to the microbiome?Blood, urine and stool samples will be collected at the above time points. Changes to the microbiome will be assessed using 16S rRNA sequencing and qPCR. Selected responders and non-responders will undergo full shotgun metagenomic and metatranscriptomic analysis. Global metabonomic profiles of serum, urine and faecal water will be generated using 1H-NMR. Further targeted analyses of bile acid and short-chain fatty acid profiles will be performed using LC-MS GC-MS. 