<qa>  What was the technique of microneurography used to record the muscle sympathetic nerve activityMuscle Sympathetic Nerve Activity. Muscle sympathetic nerve activity MSNA was directly recorded from the peroneal nerve using the technique of microneurography Roveda et al., 2003; Ueno et al., 2009. Muscle sympathetic bursts were identified by visual inspection by a single investigator, blinded to the study protocol, and were expressed as burst frequency bursts per min and burst per 100 heart beats. 