<qa>  What is the routine gene testing of EGFR, ROS1 and ALK?Some gene mutations can direct individualized treatment. The routine gene testing of EGFR, ROS1 and ALK is direct sequencing, Reverse transcription quantitative real-time polymerase chain reaction RT-QPCR and fluorescent in situ hybridization FISH or immunohistochemistry IHC. Next-generation sequencing NGS is a new technique, which is more sensitive than routine techniques. So we decided to compare the value of gene testing between routine method and NGS in EBUS-TBNA specimens and get the knowledge of how many EBUS-TBNA samples were adequate for NGS. 