<qa>  What is the amount of tissue removed during surgery?The surgeon will remove three pieces of fat tissue measuring 2 inches x 2 inches x 2 inches from the fat under the skin during the gastric restrictive surgery. In the surgical field, it is appropriate to remove this large of a piece of tissue in order to be able to extrapolate and define how much tissue will be required to be removed by punch biopsy in Phase 1 and 2 of the secondary companion study In the secondary companion study, it is expected that 100 mg subcutaneous tissue will yield 3 micrograms of total RNA. The amount of tissue removed during surgery will be greater than that amount to ensure the projected yield. The surgeon will also remove two pieces of fat tissue from the abdomen during surgery. In total, one piece will be taken from the fat tissue covering the stomach omental, one piece from just under the skin subcutaneous, and the other piece from the fat tissue covering the bowels or intestines visceral. These are fat tissues that are in the area that the surgery will be performed. The biopsy will not interfere in any way with the surgery. The surgeon will also draw a sample of blood to be used for hematology, serum chemistry, hemoglobin A1C, serum lipids, thyroid function test, serum insulin, serum hydroxybutyrate possibly elevated in MCT-fed subjects, serum leptin, and serum free fatty acid concentration. The surgeon will explain all of this to the subject prior to surgery. 