<qa>  What is the iScript cDNA synthesis kit?Molecular analysis RNA is extracted from cell cultures using RNeasy Mini Kit 74105; Qiagen, Hilden; Germany. iScript cDNA synthesis kit 1708891; Bio-Rad, CA; USA, is used for cDNA synthesis and Real-time polymerase chain reaction PCR, is performed using the iTaq Universal SYBR Green Supermix 1725124; Bio-Rad. Specific primers for each marker and for an endogenous control gene 18S rRNA is designed using Genamic Expression 1.1 software. A universal Reference RNA consisting of 10 human cancer cell lines 740000-41; Agilent as well as human genomic DNA G304A; Promega will be used in quantitative PCR qPCR reactions Statistical analysis The qPCR results will be tested according to the Kolmogorov-Smirnov test; All the reactions molecular assays, flow cytometry are performed in triplicates. A p value 0.05 is considered significant. 