<qa>  What is the ratio of 1 part anticoagulant to 9 parts whole blood?Blood and plasma Each patient will have 15 ml blood collected at enrollment. Specimens will be obtained through antecubital venipuncture. Free flow or minimal suction will be employed; vacuum containers will be avoided. Specimens for platelet-poor plasma PPP and whole blood analysis will be collected into siliconized glass tubes with 0.105M tri-sodium citrate in the ratio of 1 part anticoagulant to 9 parts whole blood. PPP will be separated within 60 minutes and analyzed immediately. Platelet-rich plasma PRP specimens will be separated from the upper 34 volume of plasma supernatant after centrifugation at 265g for 10 minutes at room temperature. The platelets will be counted on a Beckman Coulter counter and adjusted to 150 X 109 plateletsl with autologous PPP. PRP will be used within 60 minutes. Cbc, PTPTT, Fibrinogen, d-dimer, Protein C activity, Protein S activity, ATIII activity, Factor V Leiden mutation, Prothrombin G20210A mutation analysis will be performed in Memorial Herman Hospital clinical laboratories. 