<qa>  What is the final goal of the cytapheresis?To overcome the limitations described above, we plan to use cytapheresis to collect large number of PBMCs in a group of healthy individuals dispersed over a wide age-range. The collection of large number of PBMCs is essential to obtain a sufficient number of cells for each cell type to support measurements of the biomarkers of interest. The information collected will be used to identify biomarkers that change with aging in individuals who are initially healthy, independent of changes in specific PBMCs cell types. We will also develop a statistical model that can be used by other studies of biomarkers to adjust their analysis for PBMCs cell type composition without having to perform complex and expensive measures, such as flow cytometry. The data collected in PBMCs will be compared to similar biomarker data obtained from muscle and skin biopsies to understand to what extent biomarkers measured in the blood recapitulate similar changes that occur in different human tissues. Finally, once methodological limitations of measuring biomarkers in the blood have been addressed, we plan to assess the relationship of biomarkers assessed in specific circulating cell types, in the whole blood and in muscle and skin biopsies to physiological measures that typically change with aging, including measures of body composition anthropometrics, CT scan and MRI, energetics spirometry at rest and during different degrees of exercise intensity, homeostatic equilibrium hormones and inflammatory markers, neurological function neurocognitive testing, brain MRI, nerve conduction studies. At the first follow-up, Year 2 and every four years after it we will focus on in depth characterization of phenotypes that are relevant for aging. This strategy reduces the burden to participants but still allows delineating trajectories of essential variables and relate them longitudinally.The final goal is to develop new hypotheses about the biological nature of the aging process and how aging is associated with decline of physical and cognitive function. 