<qa>  What was the sample size estimated at 72 individuals?To demonstrate the non-inferiority of the strategy of sequential nephron blockade compared to dual blockade of the renin-angiotensin-aldosterone system plus bisoprolol assuming an absolute difference of 5 mmHg for systolic BP, the sample size was estimated at 72 individuals for an expected zero difference with 1 SD of 12 mmHg. 