<prompt> Diagnosis of polio -Detection of polio virus in stoolSerologyLimb wastingAFP  <response>: Ans. is a i.e., Detection of polio virus in stool Lab diagnosis of polioo Virus isolation in tissue culture is the best method for specific diagnosis.o Specimens - Blood, CSF, Throat swab, Feceso Primary monkey kidney cultures are used,o Serodiagnosis is less often employed. 