<prompt> Test used to diagnosis Pneumocystis Jirovecci -Methenamine silverPCRImmunofluorescenceAll of the above  <response>: Ans. is d i.e., All of the above Diagnosis of Pneumocystis Tirovecci Best specimen is bronchoscopy with BAL bronchoalveolar lavage fluid. Sputum can also be used. Following are methods for diagnosisi Histopathologic staining for cysts and other stages: It is most commonly used method for diagnosis. Methenamine silver, toluidine blue and crystal violet stain the cyst wall. Wright Geimsa stains all development stages.ii Immunofluorescence with monoclonal antibodies.iii PCR: It is most sensitive. 