<prompt> Which of the following is true for phosphofructokinase?It uses Fructose 1,6 bisphosphate as substrate.It generates ATPIt catalyses an irreversible reaction in glycolysisIt is activated by increased levels of ATP and citrate.  <response>: Phosphofructokinase catalyses the first committed step of glycolysis. It is the rate limiting enzyme of glycolysis. The reaction is irreversible. 