<prompt> Selective medium for Naegleria fowleri -Nutrient agar rich with E. coliNNN mediaNon Nutrient agar with E. coliDiamond media  <response>: Option 2 NNN Novy-MacNeal-Nicolle Media is used for culture of Leishmania and Trypanosoma. Option 3 CSF sample can be cultivated on nonnutrient agar Pages saline and 1.5 agar, lawn cultured with E. coli. Naegleria feeds on bacteria and crawls over the lawn culture of E. coli to produce trails. Option 4 Diamond media Axenic culture: it is used for isolation of Trichomonas and Entamoeba histolytica. 