<prompt> In estimation of bilirubin, the solution is examined at540 nm450 nm480 nm580 nm  <response>: Ans: b 450nm Ref: Vasudevan 4th edp.480The characteristic absorption maximums of some importantsubstances are:- Protein, peptide linkage- 220 nmProtein, tryptophan units- 280 nmNucleic acids- 260 nmDeoxy haemoglobin- 565 nmBilirubin- 450 nmNADH- 340 nmFAD- 450 nmFADH2- 570 nmPorphyrin- 400 nm 