<prompt> Which of the following fungi is difficult to isolate on REGULAR SDA mediaCandidaDermatophytesCryptococcusMalassezia furfur  <response>: M. furfur requires fatty acids for growth and does not grow on regular Sabourauds medium Hence is cultured on the Sabourauds dextrose agar SDA overlayed with a layer of olive oil. On incubation at 37degC, the fungus produces creamy colonies or Fried egg colonies within 5-7 days. Candida, Dermatophytes, Cryptococcus can be isolated easily on SDA. 