<prompt> Conditions required for autoclave is?121degC temperature for 20min121degC temperature for 15 min100degC temperature for 60 min100degC temperature for 90 min  <response>: ANSWER: B 12TC temperature for 15 minREF: Textbook of Microbiology by Ananthanarayan Paniker 7th edition page 27, Microbiology: A Laboratory Manual, 7th ed by Cappuccin page 283Working temperature and time for different techniques of sterilization:InstrumentPrincipleControlTempPressureTimeHot air ovenDry heatClostridiumtetani160deg C 1 hourPasteurizationMoist heatNA63deg C or0 free flowing steam30 min holders method followed by rapid cooling to 13deg C or below72deg C0 free flowing steam15-20 sec flash processfollowed by rapid cooling to 13deg C or belowTyndallizationMoist heatNA100degC0 free flowing steam20 min for 3 successive days first exposure for vegetative forms subsequest exposures for sporesKoch steam sterilizerMoist heatNA100degC0 free flowing steam90 minAutoclaveMoist heat under pressureBacillusstearother-mophillus121degC15 pounds 1 inch215 min for bacteria spores1 hour for prions 