<prompt> The following methods can be used to detect the point mutation in the beta b-globin gene that causes sickle cell anemia, exceptPolymerase chain reaction with allele-specific oligonucleotide hybridizationSouthern blot analysisDNA sequencingNohern blot analysis  <response>: The nohern blot is a technique used in molecular biology research to study gene expression by detection of RNAor isolated mRNA in a sample.The technique has been used to show overexpression of oncogenes and downregulation of tumor-suppressor genes in cancerous cells when compared to ;normal; tissue, as well as the gene expression in the rejection of transplanted organs. 