<prompt> Periplasmic space is seen in ?Gram positive bacteriaGram negative bacteriaAcid fast bacteriaAll  <response>: Ans. is b i.e., Gram negative bacteria Periplasmic space is a narrow space between cytoplasmic membrane plasma membrane and cell wall. Periplasmic space has been more frequently and better recorded in gram-negative bacteria as compared to gram-negative. At present most gram positive bacteria are thought to have only periplasm, but not periplasmic space. 