<prompt> Substrate level phosphorylation in Glycolysis is seen in which step?Glyceraldehyde 3 Phosphate dehydrogenasePyruvate kinasePhosphofructokinaseEnolase  <response>: ANSWER: B Pyruvate kinaseREF: Murray pp 130-135, Scriver, pp 1521-1552, phosphorylation is a type of metabolism that results in the formation and creation of adenosine triphosphate ATP or guanosine triphosphate GTP by the direct transfer and donation of a phosphoryi POa group to adenosine diphosphate ADP or guanosine diphosphate GDP from a phosphorylated reactive intermediate. Note that the phosphate group does not have to directly come from the substrate. By convention, the phosphoryl group that is transferred is referred to as a phosphate groupWe use the term substrate level phosphorylation because ATP formation in glycolysis is powered by the addition removal of phosphate groups phosphorylation to molecules of glucose the substrate. Hence the term substrate-level phosphorylation.Examples of substrate level phosphorylation that occurs in glycolysis is:The production of 1st ATP when 1,3 bisphophoglycerate is converted into 3-phosphoglycerate by the enzyme phosphoglycerate kinaseProduction of 2nd ATP when phosphoenolpyruvate is converted to pyruvate by the enzyme pyruvate kinase. 