<prompt> DNA Gyrase is:Eukaryotic DNA Topoisomerase IProkaryotic DNA Topoisomerase IIIProkaryotic DNA Topoisomerase IProkaryotic DNA Topoisomerase II  <response>: Topoisomerases Relieves supercoils. Cut and reseal DNA and relieve positive supercoils. Dont use ATP. Gain energy from the cleavage of the phosphodiester bond. DNA Gyrase: Counterpa of Topoisomerase II in prokaryotes. Introduces negative supercoils underwinding is known as negative supercoiling and Uses ATP Topoisomerase I Topoisomerase II Makes cut on single strand Makes cut on both strands Formation of a covalent DNA-enzyme complex preserves the free energy. ATP is not needed. ATP is needed. Relaxes negative supercoils in E. coli; Both negative and positive in Eukaryotes. Relaxes both negative positive supercoils. ve supercoil is relieved by introducing -ve supercoils. 