<prompt> A patient presents with signs of pneumonia. The bacterium obtained from sputum was a gram-positive cocci which showed alpha hemolysis on sheep agar. Which of the following test will help to confirm the diagnosis?Bile solubilityCoagulase testBacitracin testCAMP test  <response>: S. pneumoniae, however, differs from streptococci in its morphology by having a specific polysaccharide capsule, bile solubility, and optochin sensitivity.S. pneumoniae produces an autolytic enzyme amidase, which solubilizes the peptidoglycan of the cell wall; hence in old cultures, typical draughtsman colonies are formed. This autolytic activity can be augmented by surface active agents, such as bile and bile salts.Bile solubility is a constant feature of pneumococci, and is positive in all the capsulated and some noncapsulated variants.Pneumococci are catalase and oxidase negative.Parija SC. Textbook of Microbiology Immunology. Elsevier Health Sciences; 2014. Page:194-95 