<prompt> A person with prolonged usage of contact lenses presented with irritation of left eye. After examination a diagnosis of keratitis was made corneal scrapings revealed growth of pseudomonas aeroginosa. The bacteria were observed to be multidrug resistant. Which of the following best explains the mechanism of antimicrobial resistance in these isolated pseudomonas aeroginosa strainsAbility to transfer resistance genes from adjacent commensal floraImproper contact lens hygieneFrequent and injudicious use of topical antibioticsAbility of Pseudomonas to produce biofilms  <response>: D i.e. Ability of Pseudomonas to produce biofilms Contact lens wear paicularly soft lenses worn over night is the most impoant risk factor for bacterial keratitis. Pseudomonas aeruginosa is responsible for 60 of cases. Infection is more likely if there is poor lens hygiene but it can also occur even with daily disposable lenses. Bacteria may multiply in the contact lens case box where they are protacted from disinfection by bacterial biofilmQ. A corneal epithelium compromised by trauma hypoxia dt lens is also susceptible to infection. Antibiotic resistance to Pseudomonas Aeroginosa strains from contact lens wearer largely results from the ability of these strains to form slime enclosed microcolonies or biofilms. Biofilm grown cells show increased resistance to biocides and antibiotics through multiple resistance mechanism. 