<prompt> Steps of Gram stain sequence arrangement. a. Mordant- iodine treatment b. Safranin c. Crystal violet d. Alcohol washabcdbcadcadbbadc  <response>: Steps of Gram stain sequence arrangement. Step Name Feature 1 Primary stain Stain with crystal violet for 1 minute and rinse with water 2 Mordant Stain with grams iodine for 1 min 3 Decolorization Acetone for 2-3 s or 95 ethanol for 20-30s 4 Counter stain Safranin for 30-60s 