<prompt> Culture medium used for leptospira for laboratory diagnosis -Skirrows mediumEMJH mediumBYCEagarPikes medium  <response>: Ans. is b i.e., EMJH medium LeptospirosisIsolation of the organismSerological diagnosiso Leptospires can be isolated from blood or CSF during first 10 days of illnesso From urine, leptospires can be isolated for several weeks beginning at around 1 weeko Cultures most often become positive after 2 to 4 weeks range 1 week to 4 monthso Media used for isolation1 Ellinghausen - Mecullough - Johnson - Harris EMJH medium2 Fletcher medium3 Kortkofmedium4 Stuarts mediumo Leptospires grow best under aerobic conditionso Leptospires remain viable in anticoagulated blood heparin, EDTA or citrate for up to 11 days.o A fourfold or greater rise in antibody titre is diagnostico Antibodies reach detectable level in second week.o Tests used are1 Microscopic agglutination Test MAT2 ELISA3 Indirect hemagglutination test4 Macroscopic slide agglutination test 