<prompt> In SDS-PAGE Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis proteins are separated on basis of: PGI June 2009MassChargeDensityMolecular weight  <response>: Ans: A Mass D Molecular weight The most widely used method for determining purity of of a protein is SDS-PAGEfi-sodium dodecyl sulphateSDS.ln this polypeptide separates based on their relative molecular mass Mr- Harper 27th24Methods Used for Protein Separation AnalysisTechniqueProtein Property ExploitedElectrophoresisQDenaturing gel SDS-PAGE QMassQ Motecular weightNative gelMassCharge2 D gelsMolecular weight plIEFIsoelctric focussingplflsoelctric pointChromatography MethodsrIon- ExchangeChargeQGel FiltrationSizeQ or Molecular weightQAffinityBiological activityReversed phaseHydrophobicityHydrophobic InteractionHvdrophobicityChromatofocussingPlBulk MethodsQCentrifugationMassQDensityFiltrationSizeQAmmonium sulphateSolubility 