<prompt> Not a component of Gram stain -Methylene blueEthanolIodineGentian violet  <response>: GRAM STAINING 1 Primary staining with a pararosaniline dye such as crystal violet, Methyl violet,or Gentian violet 2 Application of a dilute solution of iodine . 3 Decolourisation with an inorganic solvent such as ethanol,acetone,or aniline . 4 Counterstaining with a dye of contrasting colour such as carbol fuchsin,safranine or neutral red . REF:Ananthanarayan Panickers Textbook of Microbiology 8th Edition pg no :15 