<prompt> Naeglers reaction is due to which toxin of Cl perfringens -Alpha toxinEpsilon toxinKappa toxinDelta toxin  <response>: Ans. is a i.e., Alpha toxin o Naeglers reaction detects alpha toxin phospholipase or lecithinase C. When Cl. perfringens is grown on a medium with the antitoxin spread on one half of the plate, colonies on the other half without the antitoxin will be surrounded by a zone of opacity. There will be no opacity around the colonies on the half of the plate with the antitoxin, due to the specific neutralisation of the alpha-toxin. 