<prompt> Which of the following is best to confirm diagnosis of thalassemia trait in a lady with positive family history of thalassemia?Elevated HbA2ReticulocytosisNESTROFTPeripheral smear  <response>: To Confirm diagnosis of Thalassemia trait, following can be seen HPLC of Hb Increased HbA2 levels 3.5 NESTROFT is useful in screening this disease. 