<prompt> Chlamydia are isolated by :Enzyme immune assayYolk sac inoculationDirect immunofluorescenceMicro immunofluorescence  <response>: Isolation of chlamydiae can done by :Inoculationinto yolk sacembryonated eggs of 6-8 day old chick embryo which may be pretreated by streptomycin or polymyxinInculation into experimental animals miceTissuecell culture :Preferred modeCommonly used cell lines are McCoy and HeLa cells.Cell cultures are pretreated by irradiation or chemicals such as 5-iodo 2 deoxyuridic or cycloheximide to enhance replication and detection of inclusion bodies.Pretreatment with DEAE dextrax or centrifugation after inoculation, promotes contact between chlamydiae particles and cell monolayer. 