<prompt> Two plants grown in garden soil, one to express Growth Fluoro Protein GFP and other firefly luciferase. In dark, which of the following will be seen?Plant expressing GPF will glowPlant expressing firefly luciferase will glowBoth the plants will glowBoth the plants will not glow  <response>: Firefly luciferase is by far the most commonly used bioluminescent repoer. Firefly luciferase is slightly different from GFP, a photoprotein. Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. The green fluorescent protein GFP is a protein composed of 238 amino acid residues 26.9kDa that exhibits bright green fluorescence when exposed to blue light. Photoproteins GPF dont require any special pigments, enzymes or chemicals to set it off. Once expressed, it simply needs mineral substrates to emit fluorescence. GFP was originally recruited from jellyfish. It has the same purpose as the luciferase enzyme in molecular biology, to mark the expression of select genes. At least in the lab, it GFP also requires to shine blue light or sometimes UV for it to emit the green fluorescence. Its such a standard tool now, in mice, plants, flies, fish studies. 