<prompt> True about denaturation of proteins is all exceptDisruption of secondary structure occursSequence of amino acids remain the sameBiological activity is retainedCrystallization  <response>: Denaturation is always accompanied by a loss of biological function, e.g. enzymes are inactivated.This is because biological functions of proteins depend on their higher order structures.Denaturation disrupts higher order secondary, tertiary and quaternary structure, but the primary structure amino acid sequence remains intact. 