<prompt> Opacity around colonies of Clostridium perfringens is due to:Theta toxinLecithinaseDesmolaseCytokinin  <response>: Ans. is b i.e., LecithinaseRef: Ananthanarayan, 9the, p. 251-255 and 8the, p. 252-253Naeglers reaction: This reaction is produced by C. perfringens as opalescence in 20 human serum or egg yolk media due to Lecithinase phospholipase activity of alpha toxin, it splits lecithin into phosphorylcholine and diacylgly- cerol in the presence of Caand Mg ions. 