<prompt> A large dose of EDTA is used in carbonic anhydrase enzyme inactivation. The mechanism by which EDTA acts is -It chelates with the metal ion of the enzymeIt combines with the substrate and doesnt react with the enzymeCombines with the substrate and reacts with the enzymeEnzyme-EDTA complex can not be attached to substrate  <response>: Ans. is a i.e., It chelates with ions of the enzyme EDTA is a chelating agent. It acts by causing chelation.Chelation is a chemical reaction that results in bond being formed between a metal ion and an organic Le., carbon based molecule.The resulting complex metal bound to molecule is called a chelate and contains one or more rings of atoms in which the metal ion is so firmly bound it cannot escape: The complex is stable nontoxic and is easily excreted.EDTA is a chelating agent. It is used to inactivate carbonic anhydrase enzyme.It does so by binding with the metal zinc ion of the enzyme carbonic anhydrase. 