<prompt> Transport medium for cholera -LJ mediumCary Blair mediumMYPA mediumStewart medium  <response>: Ans. is b i.e., Cary Blair medium V cholerae is not nutritionally fastidious and grows well on ordinary media. Optimum temperature for growth is 37degC range 16-40degC. They grow better in alkaline media with optimum pH 8-2. 0?5-1 NaCl is required for optimal growth 6 or above NaCl inhibits the growth.Vibrios are susceptible to heat drying but resist high alkalinity MaConkeys agar - Initial colourless colonies, later become reddish due to late lactose fermentation. Blood agar - Initially sorrounded by a zone of greening, which later become clear due to hemodigestion. In gelatin stab culture, infundibuliform funnel shaped or napiform turnip shaped liquefaction occur. Important media used for V cholerae are:-V. cholerae Transport or holding mediaEnrichment mediaPlating selective media Venkatraman - Ramakrishnan VR medium Cary-Blair medium Autoclaved sea water Alkaline petone water Monsurs taurocholatepeptone water Alkaline bile salt agar BSA Monsurs gelatin taurocholate trypticase tellurite agar GTTA TCBS medium Thiosulfate, citrate, bile salts, sucrose 