<prompt> The gold standard for the diagnosis of Wilson disease is:Liver biopsy with quantitative copper assaySerum ceruloplasmin along with free copper estimation24 hour urine copper quantificationLiver biopsy and copper staining  <response>: The gold standard for diagnosis is liver biopsy with quantitative copper assays. Affected patients have values 200 mgg dry weight of liver tissue. Copper stains are not reliable. Reference: Harrisons Principles of Internal Medicine, 18th Edition, Page 3188 