<prompt> The capsule of Cryptococcus neoformans in a CSF sample is seen byGrams stainIndia ink preparationGiemsa stainMethanamine - Silver stain  <response>: B India ink preparation In unstained, wet preparation of CSF with a drop of Indian ink or nigrosine, the capsule can be seen as a clear halo around the yeast cell. This is an example of negative staining. Diagnosis of cryptococcus is established by demonstration of capsulated, budding yeast cells in the lesions and by culture. Capsules stand out in India ink preparations. Fungus grows readily on Sabourauds agar forming smooth, mucoid, cream coloured colonies. 