<prompt> Which step in translation is inhibited by Tetracycline in prokaryotes?InitiationBinding of aminoacyl-tRNA to the A site on the ribosomePeptide-bond formationTranslocation  <response>: Binding of aminoacyl-tRNA to the A sitea. The mRNA codon at the A site determines which aminoacyl-tRNA will bind.1. The codon and the anticodon bind by base-pairing that is antiparallel see Figure below.2. Internal methionine residues in the polypeptide chain are added in response to AUG codons. They are carried by tRNAmMet , a second tRNA specific for methionine, which is not used for initiating protein synthesis.b. An elongation factor EF-Tu in prokaryotes and eEF-1 in eukaryotes and hydrolysis of GTP are required for binding.Tetracycline prevents aminoacyl-tRNA from binding to the A site on the ribosome. Streptomycin prevents the formation of the initiation complex, and erythromycin blocks translocation. Chloramphenicol will block peptide-bond formation in prokaryotic cells.Antiparallel binding of aminoacyl-tRNA to mRNA. 