<prompt> Which of the following enzymic activities would you expect to be decreased in thiamine deficiency?Pyruvate carboxylaseIsocitrate dehydrogenaseFumarasea-Ketoglutarate dehydrogenase  <response>: In the reaction catalyzed by a-ketoglutarate dehydrogenase complex, thiamine pyrophosphate, the cofactor form of thiamine, functions as a transient carrier of the five-carbon intermediate produced on decarboxylation of a-ketoglutrate. Biotin is a coenzyme of pyruvate carboxylase. Isocitrate dehydrogenase use NAD as coenzymes, whereas fumarase has no coenzyme. Ref: Brooks G.F. 2013. Chapter 6. Microbial Metabolism. In G.F. Brooks Ed,Jawetz, Melnick, Adelbergs Medical Microbiology, 26e. 