<prompt> Activity of which of the following enzyme is NOT affected by insulin:Pyruvate kinaseGlycogen synthaseHexokinaseGlucokinase  <response>: HexokinaseTransfer Phosphate group from ATP to Glucose.Has high affinity for glucose Or Lower Km and not affected by insulin.Mg2 is the cofactor.Irreversible step.ATP is utilized.GlucokinasePresent in Liver and Pancreatic β cells.Has low affinity for Glucose. High Km.Hence acts only when blood glucose is very high 100 mgdl.Induced by Insulin following a meal.Play an important role in regulation of blood glucose.In the liver, function of Glucokinase is to remove glucose from portal vein following a mealIn the beta cells of Pancreas, function of Glucokinase is to release Insulin. Glycogen SynthaseInsulin favor Glycogenesis by dephosphorylating, Glycogen Synthase.Key concept: 