<prompt> Diganosis of polio -Detection of polio virus in stoolSerologyLimb wastingAFP  <response>: As fecal excretion may be intermittent ,the best results are obtained by testing fecal samples collected on two seperate days as early in the illness as possible. REF:ANANTHANARAYAN AND PANIKERS TEXTBOOK OF MICROBIOLOGY 9TH EDITION PAGE NO:487 