<prompt> True regarding electrophoresis are all exceptIsoelectric focusing use ampholytes pIAdversely affect structure functionDepends on size not on shapeCommonest method of purifying protein  <response>: Electrophoresis depends on size as well as on shapeElectrophoresis is the process that uses an electric field to drive the movement of charged particles. Electrophoresis depends on the net charge of the particle, molecular weight, pH of the medium, the strength of the electric field and temperature.About other optionsAmpholyte is used in isoelectric focusing to create a pH gradient across the supporting media. Molecules stop migrating as soon as they reach their respective isoelectric point pI.Electrophoresis can adversely affect the structure and function of proteins.Electrophoresis is the most common method for protein separation.Electrophoresis is the most common method of protein separation in the clinical laboratory. PAGE and SDS-PAGE are extensively used for characterization and purification of tissue proteins, enzyme, receptors, and surface antigens.Electrophoresis uses an electric field 