<prompt> A 16 yr old female presents with Primary Amenorrhea. Examination shows a Sho Blind Vagina, with absent Uterus. The Next Investigation of choice is :KaryotypingIVPGonadotrophin levelsSerum Prolactin  <response>: Karyotyping A female phenotype presenting with absent uterus and sho blind vagina : Friend what are the DDs which come to your mind. I can think of only 2 : Mullerian dysgenesis and Testicular feminizing syndrome or male pseudo hermaphroditism. So, the best way to differentiate between the two is to do karyotyping or chromatinbarr body test. Which comes positive barr body present in 46XX and negative barr body absent in case of 46XY Genotype. 