<prompt> In ;Hotair oven; for holding period of one hour, temperature required is -100o C120o C140o C160 o C  <response>: Temperature and time: 160 o C for 2 hours holding time is required for sterilization However alternative temperatures and holding time include 170degC for 1 hour and 180degC for 30 minutes Ref: a textbook of Microbiology Dr.C.P.Baveja 4th Edition pg no:30 