<prompt> Which of the following statement is true regarding Acanthamoeba keratitis?For the isolation of the causative agent, corneal scraping should be cultured on a nutrient agar plateThe causative agent, Acanthamoeba is a helminth whose normal habitat is soilKeratitis due to Acanthamoeba is not seen in the immunocompromised hostAcanthamoeba does not depend upon a human host for the completion of its life-cycle  <response>: For the isolation of causative agent, corneal scraping should be cultured on non-nutrient agar not nutrient agar. Culture on non-nutrient agar E. coli enriched may show trophozoites within 48 hrs : which gradually turn into yeasts. - khuranaNormal habitat is soil, water and dust but it is an amoeba not a helminthKeratitis due to Acanthamoeba may be seen both in immunocompetent and immunocompromised hosts.In contrast to many another pathogenic amoeba, this organism is not naturally parasitic and does not require a host. 