<prompt> Staining technique for cryptosporodium: September 2012Indian inkModified acid fastGiemsaMethylene blue  <response>: Ans. B i.e. Modified acid fast Staining of cryptosporidium Traditional parasitology stains e.g., Giemsa are of limited value. They do not differentiate between oocysts and similarly-sized fecal yeasts the main differential diagnosis of Cryptosporidium in microscopy and other debris. Modified acid-fast staining technique is a simple and effective method: the oocysts stain bright red against a background of blue-green fecal debris and yeasts. The acid-fast staining technique has been modified and improved, including: hot and cold modified acid-fast stains; incorporation of dimethyl sulfoxide DMSO; and incorporation of the detergent tergitol. 