<prompt> Which of the following is required to bring about gluconeogenesis from pyruvate?Pyruvate dehydrogenaseBiotinAlpha ketoglutarate dehydrogenaseFructose-6 phosphate  <response>: Pyruvate in the cytoplasm enters the mitochondria. Then, carboxylation of pyruvate to oxaloacetate is catalysed by a mitochondrial enzyme, pyruvate carboxylase Fig. 9.24. It needs the co-enzymes biotin and ATP.Ref: DM Vasudevan, 7th edition, page no: 118 