<prompt> Diagnosis of beta Thalassemia is established byNESTROFT TestHb A1c estimationHb electrophoresisTarget cells in peripheral smear  <response>: Diagnostic test for thalassemia is Hb electrophoresis. NESTROFT Test - Naked Eye Single Tube Red Cell Osmotic Fragility Test - used for screening for Thalassemia HbA1c - Used to assess long term glycemic control in diabetics blood sugar control over past 3 months Target cells - A feature of thalassemia, but not diagnostic Reference : Ghai essential of pediatrics, eighth edition, p.no:342 