<prompt> In pregnancy with increased MSAFP which of the following should be done?Repeat measurement of MSAFP at later dateUSGAmniocentesisTermination of pregnancy  <response>: Maternal Serum Alpha Fetoprotein MSAFP estimation at 16 weeks was used as a screening method for open neural tube defects but today,has been almost completely replaced by ultrasonography. Refer page no 486 of Text book of obstetrics,sheila balakrishnan,2 nd edition. 