<prompt> Poliomyelitis is diagnosed by: September 2010 March 2013 a, hClinical presentationAntibody titer measurement in bloodIsolation of virus from bloodIsolation of virus from stool  <response>: Ans. D: Isolation of virus from stool Poliomyelitis diagnosis may be confirmed with a laboratory diagnosis. Viral isolation Poliovirus may be recovered from the stool or pharynx of a person with presumed poliomyelitis. Isolation of virus from the cerebrospinal fluid CSF is diagnostic, but is rarely accomplished. If poliovirus is isolated from a person with acute flaccid paralysis, it must be tested fuher, using oligonucleotide mapping fingerprinting or genomic sequencing, to determine if the virus is wild-like or vaccine-like. Serology Neutralizing antibodies appear early and may be at high levels by the time the patient is hospitalized and, therefore, a 4-fold rise may not be demonstrated. Cerebrospinal fluid CSF The CSF in poliovirus infection usually contains an increased number of white blood cells 10 to 200 cellsmm 3, primarily lymphocytes and a mildly elevated protein from 40 to 50 mg100 ml. 