<prompt> Itos test is used in the diagnosis of the following infection:GonorrhoeaHerpesChancroidGranuloma venereum  <response>: The Ito Reenstierna test was developed for detecting Haemophilus ducreyi using a commercial antigen by intradermal testing and proved positive in 90 of cases. Gram staining shows school of fish or railroad track appearance which is characteristic. Nucleic acid amplification tests are now the optimal method of diagnosing H. ducreyi. Ref: Oxford Textbook of Medicine: Infection edited by David Warrell, Timothy M. Cox, John Fih, Estee Torok, 2012, 375-376. 