<prompt> Edmans reagent is2-4 dinitrophenol1-fluoro-2, 4-dinitrobenzenePhenyl-isocyanateCynogen bromide  <response>: Ans. is c i.e., Phenyl-isocyanateDetermination of amino acid sequence sequence analysis is performed by Edman degradation method on automatic mechines, called cyclic sequentors. Edmans reagent is phenyl-isothiocyanate, and forms a covalent bond to N-terminal of amino-acid. This can be identified.Sangers reagent 1-fluoro-2,4-dinitrobenzene can be used in place of Edmans reagent. 