<prompt> Virus growth in cell cultures can be detected by the following methods EXCEPTCytopathic effectTransformationInterferenceGram staining  <response>: D. i.e. Gram staining 440 -41 - Ananthanarayan 7th Detection of virus growth in cell culture bv following methods1. Cytopathic effect2. Metabolic inhibition3. Hemadsorption Influenza, and Parainfluenza4. Interference5. Transformation6. Immufluorescence Three methods are employed for the cultivation of virus - 1 inoculation into animals, embryonated eggs or tissue culture. Three types of tissue cultures are - organ culture, Explant culture, cell culture Primary cell cultures,Diploid cell strains, continuous cell linesPlaque formation in a virus occurs because of - Infectivity of the virusIn reverse transcriptase polymerase chain reaction diagnosis is formed by - Retrovirus Syncytium formation is characteristic feature of cytopathic effect Von-magnus-phenomenon - virus yield has high haemagglotination titre but low infectivity 