<prompt> An organism with sterols in their cell wall isMycoplasmaBacillusStreptococcusSpirillum  <response>: Mycoplasma lacks a rigid cell wall and they are bounded by a triple-layered unit membrane that contains sterols. Mycoplasma PPLO - by Nocard Roux Smallest free-living organism Gram-negative.highly pleomorphic organism,stained by Giemsa Found in the form of elementary bodies filaments of various size Facultative anaerobe, require a source of cholesterol for growth Media hayflick media, Eatons media Colonies biphasic with a fried egg appearance CF: Primary atypical pneumonia. Bullous myringitis.Otitis Lab diagnosis: 1. Isolation of organism - from throat swab, respiratory secretion 2. serological diagnosis -1.Specific - HI, IF. metabolic inhibition 2. Nonspecific- streptococcus MG 3.Cold agglutination Treatment: DOC- Erythromycin Presence of 70 S ribosome makes it susceptible to antibiotics that inhibit protein synthesis;but due to the lack of cell wall they are resistant to penicillin Remember Ureaplasma ureolyticum belongs to the family mycoplasmatacea; which can hydrolyse urea is a cause for nongonococcal urethritis L forms of bacteria - are due to the loss of bacteria but they retain the biochemical antigenic properties of present bacteria. 