<prompt> The capsule of cryptococcus neoformans in a CSF sample is best seen by -Gram stainIndia ink preparationGiemsa stainMethenamine-silver stain  <response>: Cryptococcus is the capsulated yeast.Among the given staining techniques, India ink preparation is the best staining technique used for demonstration of capsule negative staining - sensitivity of the technique: 6075.Other capsular staining techniques are:10 Nigrosin stainingModified India ink preparation with 2 chromium mercuryAlcian blue stainingMethanamine silver and Periodic acid- Schiff used for tissue sample.Sensitivity of various diagnostic tests- Harrison 18e p1652Cryptococcal antigen detection in CSF90Blood culture: 1030 in non-HIV patients and 60 in HIV patientsSputum culture: 10Sputum antigen detection: 30 