<prompt> Fried Egg colony is seen in culture of -MycoplasmaLegionellaTrachomaHemophilus  <response>: Ans. is a i.e., Mycoplasma Culture of Mycoplasma May be cultivated in fluid or solid media. Generally facultativeanaerobes,growthbeingbetteraerobically. But,sinceorganismsfromprimarytissuespecimens frequently grow under anaerobic conditions, an atmosphere of 95 N7 and 5 CO2 is preferred. Mycoplasma and ureoplasma do not produce turbidity in liquid medium. Grow in heart infusion peptone broth with 2 agar pH 7.8 P.P.L.O. broth with fresh yeast extract and horse serum. For enrichement, 30 human ascitic fluid or animal serum horse, rabbit are added to the medium. Serum acts as a source of cholesterol. Most mycoplasma colonies are hemolytic - hemolysis due to production of hydrogen peroxide. The colony gives fried eggf appearance. Colonies are best studied after staining by Dienes method.Remember Dienes phenomenon is used to detect swarming of proteus. 