<prompt> The peripheral blood smear of a patient shows features of thalasemia, also presented with anaemia. Family history is also ve. The investigation done to establish the diagnosis is :ESR estimationBlood spherocyte estimationBone marrow aspirationHb-electrophoresis  <response>: Ans. is d i.e. Hb- electrophoresisElectrophoretic techniques are used for routine hemoglobin analysis. It helps in quantification and identification of various hemoglobins.As we all are aware that thalassemia is a quantitative defect in Hb characterized by lack of certain types of hemoglobin and compensatory increase in other hemoglobin, therefore electrophoresis proves to be a reliable method for screening in thalassemia. 