<prompt> A 45 years female complains of lower abdominal pain and vaginal discharge. On examination there is cervicitis along with a mucopurulent cervical discharge. The gram smear of the discharge shows presence of abundant pus cells but no bacteria. The best approach to isolates the possible causative agent would be:Culture on chocolate agar supplemented with HaeminCulture on McCoy cellsCulture on a bilayer human blood agarCulture on vero cell lines  <response>: Ans. is b i.e., Culture on McCoy cellsRef: Harrison, 19the, p. 1165 and Ananthanarayan, 9the, p. 415Chlamydiae are usually grown on McCoy cell line and HeLa cell line.Genital chlamydiasis is suspected if gram stained smear of urogenital exudates shows significant number of neutrophils. 4oil immersion field in urethritis, 30 in cervicitis in the absence of gonococcal bacteria. 