<prompt> Which of the following is used for sequencing a long DNA fragment :Sangers techniqueChain termination methodChromosome WalkingRFLP  <response>: Chromosome walking Sequencing method of choice for sequencing long DNA fragments between 1.3 and 7 kb Applications of chromosome walking: Positional cloning Find, isolate, and clone a paicular allele Isolate a locus of interest for which no probe is available Analysis of genetically transmitted diseases Detect mutations Study SNPs Single Nucleotide Polymorphisms Disadvantage : Speed of this technique cant be enhanced 