<prompt> The rate limiting enzyme in glycolysis isGlucose 6- dehydrogenasePhosphofructokinaseGlucokinasePyruvate kinase  <response>: Phosphofroctokinase 152- Harper 27th 108- LippincottsPhosphofroctokinase - is the pace- setting rate- limiting enzyme of glycolysis. It is inhibited by ATP and citrate uses fructose 6- phosphate as substrate, and catalyzes a reaction that is for from equilibrium. The reaction is activated by fructose 2- 6 biphosphate 