<prompt> Steps of PCR in sequence are ?Extend DNA, Anneal Primers, Denature DNAAnneal Primers, Extend DNA, Denature DNADenature DNA, Anneal Primers, Extend DNADenature DNA, Extend DNA, Anneal Primers  <response>: PCR - Method of amplifying a target sequence of DNA developed by Karry Mullis in 1984 Essential requirements of a PCR reaction: Targeted DNA Two primers Four deoxyribonucleotide Thermostable DNA polymerase Steps of PCR: 1. Denaturation: Two DNA strands separated 94o C 2. Annealing: Addition of primers one for each strand 60oC 3. ExtensionElongation: Polymerization or synthesis of DNA. 72oC These cycles of Denaturation, Annealing, and Extension are repeated again and again to synthesize large amounts of DNA. 