<prompt> On blood agar target appearance of Cl. perfringens is due to which toxin -LecithinasePhospholipasea-toxinAll of the above  <response>: Ans. is d i.e., All of the above Two important characteristic feature of Cl. perfringens are1 Target hemolysis double zone hemolysis on blood agar. It is a narrow zone of complete hemolysis by theta toxin which is surrounded by a wider incomplete hemolysis by alpha-toxin.2 Naeglers reaction detects alpha toxin phospholipase or lecithinase C. When Cl. perfringens is grown on a medium with the antitoxin spread on one half of the plate, colonies on the other half without the antitoxin will be surrounded by a zone of opacity. There will be no opacity around the colonies on the half of the plate with the antitoxin, due to the specific neutralisation of the alpha-toxin. Alpha toxin is phospholipase lecithinase-C. 