<prompt> RFLP, true isare:Detects mutationRecognizes triuncleotide repeatesDetects deletionAll of the above  <response>: A i.e. Detects Mutation B i.e., Recognizes trinucleotide repeats C i.e., Detects deletion- RFLP is a DNA sequence variation observed by cleaving the DNA into fragments by restriction endonuclease RE enzymeQ. These restriction fragments are separated by agarose gel electrophoresis on the basis of size and detected by southern blotQ.- RFLP results from single nucleotide polymorphism SNPs, single base changes mutation, deletions, inseions copy number variations CNVs, length polymorphism variable number of tandem repeats VNTR, like mini satellite repeats and microsatellite or sho tandem repeats STR. All of these result in gain loss of a restriction site; thereby producing an extrafewer fragments.- RE cuts both strands of double stranded ds DNA at specific restriction sites with palindromic inverse repeat arrangement; thus producing smaller, manageable fragments with sho sequenes and sticky cohesive, overlapping, staggered blunt endsQ. These restriction fragments can be isolated by agarose gel polyacrylamide - electrophoresisQ or HPLC.RFLP is used for identification of gene for genomic mapingQ, establishing linkage thus analyzing chromosomal structure and measurement identification of DNA fragments or molecules of different size, which by process of chromosomal walking will eventually define disease locus and isolate any gene in unknown location of large DNA. 