<prompt> Free cholesterol is mostly found in?Outer layer of membraneCytosolBoth sides of plasma membraneMitochondria  <response>: Cholesterol is amphipathic in nature Cholesterol is an impoant component of membranes present on both sides of the plasma membrane Cholesterol intercalates among the phospholipids of the membrane, with its hydrophilic hydroxyl group at the aqueous interface and the remainder of the molecule buried within the lipid bilayer Cholesterol can also be combined with long-chain fatty acids to form cholesteryl ester .. Reference Harpers Illustrated Biochemistry 30th Edition Pg 267 