<prompt> Protein purification and separation can be done by all exceptChromatographyCentrifugationElectrophoresisNone  <response>: Techniques for protein separation:ChromatographyColumn ChromatographyPaition ChromatographySize Exclusion ChromatographyAdsorption ChromatographyIon Exchange ChromatographyAffinity ChromatographyHigh-Pressure Liquid ChromatographyGas ChromatographyPAGEIEFSalting out with ammonia sulfateRef: Harper 28th Ed. Page-21, Harper 27th Ed. Page: 21 