<prompt> All of the following are used in Grams staining except?IodineAlcoholCongo redCrystal violet  <response>: GRAM STAINING 1Primary staining with a pararosaniline dye such as crystal violet, methyl violet, or gentian violet 2Application of a dilute solution of iodine 3Decolourisation with an inorganic solvent such as ethanol, acetone, or aniline 4Counterstaining with a dye of contrasting color such as carbon fuchsin, safranine or neutral red Congo Red: It is an organic dye, an azo dye mainly used in the diagnosis of amyloidosis REF:Ananthanarayan Panickers Textbook of Microbiology 9th Edition pg no: 13 