<prompt> Mechanism action of Botulinum toxin is byIncreased cAMPIncreased cGMPInhibition of acetylcholine releaseInhibition of noradrenaline release  <response>: C Inhibition of acetylcholine release Botulinum toxin BTX is a neurotoxic protein produced by Clostridium botulinums. It prevents release of the neurotransmitter acetylcholine from axon endings at the neuromuscular junction thus causes flaccid paralysis. Infection with the bacterium causes the disease botulism. Toxin is also used commercially in medicine, cosmetics and research. Botulinum is the most acutely lethal toxin known, with an estimated human median lethal dose LD50 of 1.3-2.1 ng kg intravenously or intramuscularly and 10-13 ngkg when inhaled. Botulinum toxin is used to treat Overactive muscle movement, including post-stroke spasticity, post-spinal cord injury spasticity, spasms of the head and neck, eyelid, vagina, limbs, jaw, and vocal cords. To relax clenching of muscles, including those of the oesophagus, jaw, lower urinary tract and bladder, or clenching of the anus which can exacerbate anal fissure. Used for improper eye alignment In the treatment correction of Strabismus Prophylactic treatment of Chronic migraine headache. Effective for reduction of facial wrinkles Used to treat disorders of hyperactive nerves including excessive sweating, neuropathic pain, and some allergy symptoms Evaluated for use in treating chronic pain 