<prompt> A 45-year-old female complains of lower abdominal pain and vaginal discharge. On examination, there is cervicitis along with a mucopurulent cervical discharge. The Gram smear of the discharge shows presence of abundant pus cells, but no bacteria.The best approach to isolate the possible causative agent would be :Culture on chocholate agar supplemented with heminCulture on McCoy cellsCulture on a bilayer human blood agarCulture on Vero cell lines  <response>: Chlamydial infection Presenting symptoms: dysuria,dysparenuia,postcoital bleeding,intermenstrual bleeding Findings: mucopurulent cervical discharge,cervical edema,cervical ectopy,cervical friability McCoy cell monolayers are used for tissue culture due to its 100 specificity. It takes 3-7 days to obtain result. D.C.DUTTAS TEXTBOOK OF GYNAECOLOGY,Pg no;150,6th edition 