<prompt> Proteins are separated on the basis of charge in -SDS-PAGEUltracentrifugationAffinity chromatographyHPLC  <response>: Ans. is d i.e., HPLC Property of protein usedMethodProtein solubilitySalting out or salt extraction precipitation of protein by ammonium sulfateMolecular sizeo Gel filtration chromatography molecular-sieve chromatography or size exclusion chromatographyo Ultracentrifugationo SDS-PAGE Sodium dodecyi sulphate-polyacrylamide gel electrophoresiso DialysisMolecular charge Ionic chargeo Ion exchange chromatographyo High performance liquid chromatography HPLCo Electrophoresis simple on cellulose acetate starch gelo Isoelectric focusing a variant of electrophoresisMolecular charge and molecular weightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity chromatographyHydrophobicityHydrophobic interaction chromatography 