<prompt> In glycolysis the first commited step is catalysed by:2,3 DPGGlucokinaseHexokinasePFK  <response>: Fructose 6 Phosphate is phosphorylated to Fructose 1, 6 Bisphosphate by Phosphofructokinase I PFK-ISecond irreversible stepMajor regulatory step of GlycolysisCommitted step of GIycolysis because once Fructose1, 6 Bisphosphate is formed it should undergo glycolysisOtherwise called bottle neck of Glycolysis1 ATP is utilized.Key Concept:First Committed step is catalyzed by PhosphofructokinaseThis is otherwise called the bottle neck of Glycolysis. 