<prompt> In the given chromatography, the negative charged compound used is:SephadexCMC carboxy methyl celluloseDEAE cellulose Di Ethyl Amino Ethyl celluloseMethyl cellulose media  <response>: This is ion exchange chromatography shown. In ion exchange chromatography: Either positive charge can be used on the column or negative charge. Carboxy group always gives negative charge because carboxy -COOH ionize to give COO-, E.g.CMC i.e. carboxy methyl cellulose is negatively charged. Amino group NH2 always gives positive charge as NH2 ionize to give NH3. E.g. DEAE cellulose Di Ethyl Amino Ethyl cellulose positively charged. NOTE: The best method to measure HBA1C is ion-exchange chromatography. 