<prompt> Which test uses oligomer with single base pair substitution as primerPCRRFLPError coded mutation analysis with PCRSite directed mutagenesis  <response>: D i.e. Site directed mutagenesis Site directed mutagenesis and oligonucleotide directed mutagenesis uses oligomer with single base pair substitution as primerQ to create a specific DNA sequence change in gene, thus producing modified proteins with changes in primary structure of protein. This helps researchers to examine the effect of these changes on 3-D structure, folding activity of protein. 