<prompt> Suckling mice is used for isolation ofCoxsackie virusPoxHerpesAdenovirus  <response>: It is necessary to employ suckling mice for the isolation of coxsackieviruses. Inoculation is usually made by intracerebral, subcutaneous and intraperitoneal route. Adult mice are not susceptible. Human diploid fibroblasts HDF cell line is used for cultivation of coxsackie A7,9,16; CMV; Echovirus; enteroviruses; HSV-1,2; Poliovirus: parainfluenzavirus, rhinovirus and VZV.Cultivation of virusesAs viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium. Threemethods are employed for cultivation of viruses :1. Animal Inoculation Earliest method Mice are most widely employed animals Other animals used are guinea pigs, rabbits and ferrets.2. Embryonated eggs Sites of inoculation are:a Chorioallantoic Membrane --- visible pocks are produced HSV, Vaccinia, Smallpoxb Amniotic Sacc Allantoic cavity Influenza o Some paramyxovirusesd Yolk Sac o Some viruses o Chlamydia o Rickettsia3.Tissue culture Most important method for virus isolation. Three types of tissue culture are availableI. Organ CultureII Explant CultureIII. Cell Culture - routinely employed Cell culture may be1. Primary cell culture: Rhesus monkey kidney, human amnion, chick embryo fibroblast, human embryonic kidney.2. Diploid cell strains:- Human diploid fibroblast HL-8, WI-38.3. Continuous cell line:- Hela, HEP-2, McCoy, Vero 