<prompt> The teiary structure of protein is detected by:X- ray diffraction crystallographs.SpectrophotometryElectrophoresis.Chromatography.  <response>: High performance pressure liquid chromatography HPLC is a modern refinement of column chromatography and is used for protein separation, purification and identification. It can not determine protein structure.Electrophoresis separates purifies proteins based on migration of charged proteins in an electric field. It can not determine protein structure.Higher level 2deg, 3deg 4deg of protein structure is studied by X-ray crystallography, NMR sepectroscopy, infrared and ultraviolet spectroscopy. 