<prompt> Enzyme use in ELISA -Alkaline phosphataseAcid phosphataseGlucosidaseGlycosyl transferase  <response>: A Alkaline Phosphate 64- Harper 29th 730 U. Satyanarayana 4thELlSAs use antibodies covalently linked to a reporter enzyme such as alkaline phosphatase or horseradish peroxidase whose products are readily detected, generally by the absorbance of light or by flourescence 64- Harper 24th ELISA is based on the immunochemical principles of antigen-antibody reactionApplications:1. Determination of small quantities of proteins hormones antigen, antibodies and other biological substances.2. Most commonly used pregnancy test for the defection of hCG in urine is based on ELISA, by this test pregnancy can be detected within few days after conception.3. Diagnosis of AIDS. 