<prompt> A male infant presented with distension of abdomen shortly after birth with the passing of less meconium. Subsequently, a fullthickness biopsy of the rectum was performed. The rectal biopsy is likely to showFibrosis of submucosaLack of ganglion cellsThickened muscularis propriaHyalinisation of the muscular coat  <response>: Whenever neonate presents with delayed passage of stool, Hirschsprungs disease should be suspected.Diagnosis of Hirschsprungs diseaseThe diagnosis of Hirschsprungs disease is confirmed by rectal suction biopsiesRectal suction biopsies demonstrate Hypertrophied nerve bundlesThe absence of ganglion cellsOther diagnostic procedures in Hirschsprungs diseaseRectal manometryAnorectal manometry measures the pressure of internal anal sphincter while a balloon is distended in the rectum. The results are interpreted as Normal individual Reflex decline in internal sphincter pressure.Hirschsprungs disease Pressure fails to drop, the Paradoxical rise in pressure with rectal distention.Radiography Barium enemaIt demonstrates the presence of the transition zone between the normal dilated proximal colon and a smaller calibre obstructed distal colon caused by non-relaxation of aganglionic bowel.Delayed passage of stool meconium and abdominal distension suggests low intestinal obstruction. 