<prompt> Not used in grams staining?Methylene blueCrystla violetIodineSafranin  <response>: GRAM STAINING 1primary staining with a pararosaniline dye such as crystal violet, methyl violet, or gentian violet 2application of a dilute solution of iodine 3 decolorization with an inorganic solvent such as ethanol, acetone, or aniline 4counterstaining with a dye of contrasting color such as carbon fuchsin, safranine or neutral red REF:Ananthanarayan Panickers Textbook of Microbiology 8th Edition pg no: 15 