<prompt> Protein separation based on moleular size -HPLCGel filtration chromatographySalting outAffinity chromatography  <response>: Ans. is b i.e., Gel filtration chromatographyProperty of protein usedMethodProtein solubilitySalting out or salt extraction precipitation of protein by ammonium sulfateMolecular sizeo Gel filtration chromatography molecular-sieve chromatography or size exclusion chromatographyo Ultracentrifugationo SDS-PAGE Sodium dodecyl sulphate-polyacrylamide gel electrophoresiso DialysisMolecular charge Ionic chargeo Ion exchange chromatographyo High performance liquid chromatography HPLCo Electrophoresis simple on cellulose acetate starch gelo Isoelectric focusing a variant of electrophoresisMolecular charge and molecular weightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity chromatographyHydrophobicityHydrophobic interaction chromatography 