<prompt> Diagnosis of Dengue fever can be made earliest by-Viral cultureNS - 1 antigen detectionIgG antibody detectionNucleic acid test  <response>: Ans. is b i.e., NS-1 antigen detection Dengue diagnostics and sample characteristics Clinical sampleDiagnostic methodMethodologTime to result Viral isolationMosquito or mosquito cell culture inoculationOne week or moreVirusDetectionand itscomponentsAcute Serum1-5 days of feverandnecropsy tissues Nucleic acid detectionRT-PCR and real time RT-PCR1 or 2 days NS1 Ag rapid testsMinutes Antigen detectionNS I Ag ELISA1 day I mmuno-histochemistry2-5 days Paired sera acute serum from 1-5 days and second serum 15-21 days afterIgM or IgG seroconversionELISAHIA1-2 daysSerologicalresponseNeutralization testMinimum 7 days IgM detectionrecent infectionELBA1 or 2 daysSerum after day 5 of feverRapid testsMinutesIgG detectionIgG ELISAHIA1 or 2 days 