<prompt> Gluconeogenesis is mainly seen in:KidneyLiverSpleenHeart  <response>: Ans. b LiverRef: Vasudevan Biochemistry 8e. P 136SITE OF GLUCONEOGENESIS Major site of gluconeogenesis: Liver 90 Secondary site: Kidney cortex and in small intestine under some conditions. 10 It takes place in the mitochondria and cytoplasm. The production of glucose is necessary for use as a fuel source by the brain, testes, erythrocytes, kidney medulla, lens and cornea of the eye and exercising muscles. 