<prompt> Which of the following enzymes, requires Biotin as the coenzymePyruvate DehydrogenasePyruvate KinasePyruvate CarboxylasePEP Carboxy Kinase  <response>: Pyruvate enters the mitochondrion and is carboxylated to oxaloacetate by pyruvate carboxylase, an ATP-dependent reaction in which the coenzyme is the Vitamin biotin.Ref: Harper;s Biochemistry; 30th edition; Chapter 16; The Citric Acid Cycle: The Central Pathway of Carbohydrate, Lipid Amino Acid Metabolism 