<prompt> Which of the following enzymes is used in Recombinant DNA research for Homopolymer tailing?Reverse transcriptaseS1 TransferasePolynucleotide kinaseTerminal transferase.  <response>: Ans. D. Terminal transferaseSome of the Enzymes Used in Recombinant DNA ResearchEnzymeReactionPrimary UseAlkaline phosphataseDephosphorylates 5 ends of RNA and DNARemoval of 5-PO4 groups prior to kinase labeling; also used to prevent self-ligationBAL 31 nucleaseDegrades both the 3 and 5 ends of DNAProgressive shortening of DNA moleculesDNA ligaseCatalyses bonds between DNA moleculesJoining of DNA moleculesDNA polymerase ISynthesizes double-stranded DNA from single- stranded DNASynthesis of double-stranded cDNA; nick translation; generation of blunt ends from sticky endsDNase IUnder appropriate conditions, produces single- stranded nicks in DNANick translation; mapping of hypersensitive sites; mapping protein-DNA interactionsExonuclease IIIRemoves nucleotides from 3 ends of DNADNA sequencing; mapping of DNA-protein interactionsExonucleaseRemoves nucleotides from 5 ends of DNADNA sequencingPolynucleotide kinaseTransfers terminal phosphate position from ATP to 5-OH groups of DNA or RNA32P end-labeling of DNA or RNAReverse transcriptaseSynthesizes DNA from RNA templateSynthesis of cDNA from mRNA; RNA 5 end mapping studiesS1 nucleaseDegrades single-stranded DNARemoval of hairpin in synthesis of cDNA; RNA mapping studies both 5 and 3 endsTerminal transferaseAdds nucleotides to the 3 ends of DNAHomopolymer tailing 