<prompt> Which of the following is a cause of intravascular hemolysisCold agglutinin diseaseWarm type autoimmune hemolytic anaemiaPNHBoth bc  <response>: PNH Paroxysmal nocturnal hemoglobinura PNH is an acquired intrinsic defect in RBC membrane Its due to mutation in PIGA gene phosphatidyl inositol glycan complementation group A PNH red cell are deficient in :- CD55 or decay accelerating factor CD 59 or membrane inhibitor of reactive lysis C8 binding protein Characterised by pancytopenia, hemolysis and thrombosis Diagnosed by FLOWCYTOMETRIC ANALYSIS Thrombosis is the leading cause of disease related death Ref:- Robbins Basic Pathology 10th Edition; Pg num:- 642,643 