<prompt> Which of the following is used in gene therapy?ElectroporationElectrofocusingSelectively targeted recombinationIntracytoplasmic injection  <response>: Electroporation is used in gene therapy. Gene therapy is a technique that uses genes to treat or prevent disease. Delivery of DNA into patient;s cells by using recombinant virusesnaked DNADNA complexes. Methods used include intranuclear injection, electroporation, gene-gun, sonoporation, and magnetofection Gene therapy was first used for severe combined immunodeficiency disorder SCID, which is due to adenosine deaminase enzyme deficiency. FDA-approved gene therapy is used for SCID, Leber;s congenital amaurosis, multiple myeloma and adrenoleukodystrophy Ref: Nelson textbook of pediatrics 21st edition Pgno: 584-586 