<prompt> Major bacterial enzyme responsible for putrefaction?HyaluronidaseLecithinaseMetalloproteinaseCollagenase  <response>: Ans. is b i.e., Lecithinase Putrefaction Produced mainly by the action of bacterial enzymes lecithinase -- Cl. welchii Anaerobic organism from bowels Causing marked hemolysis and liquefaction in blood vessels and tissue spaces, thus producing following effects Color changes : greenish discoloration in right iliac fossa abdomen -- genitalia -4 chest -- neck -- face - arms -4 legs. Superficial veins over the root of the limbs, thighs, sides of abdomen, shoulder, chest and neck are stained greenish-brown or purplish-red, marbled appearance in 36 to 48 hours marbling. Foul-smelling and breakdown of proteins and carbohydrates. Gases produced : CO2 NH3 CH4 mercaptans mercaptans noninflammable H2S inflammable. Gas bubbles cause crepitation and give spongelike feeling. Blood-stained froth from mouth and nostrils is called postmoem purge. Distension of body 36 to 48 hours Anus and uterus prolapse : 2 to 3 days PM blisters, face distroted : more than 3 days Nail, skull sutures, and hair loss : more than 5 days Skin shows slippage, comes off in glove and stocking fashion. Postmoem luminescence due to : Photobacterium fischeri bacteria and Armillaria mellea fungi. 