<prompt> Indirect Coomb;s test detects:Antibodies attached to RBC SurfaceAntibodies in the serumAntigens attached to RBC SurfaceAntigens in the serum  <response>: Indirect Coomb;s test detects IgG antibodies in the serum e.g. Anti-D Antibodies. Direct Coomb;s test detects IgG Antibodies or complements attached to the surface of RBCs. Direct Antihuman Globulin Test Detects RBCs sensitized with IgG or ComplementsC3B or C3d DAT; Coomb;s; Direct Coomb;s Detects IgG Antibodies or complements attached to the surface of RBCs. Indirect Antihuman Globulin Test Direct Coomb;s Test In the Direct Coomb;s test, red blood cells RBCs sensitized with IgG antibodies or C3b, C3d are agglutinated when Coomb;s reagent Rabbit Anti-IgG antibody is added to the test tube Detects Antibodies in the Serum Indirect Coomb;s Test In the Indirect Coomb;s test IgG antibodies e.g. Anti-D in the serum must first bind to blood group Type 0 Test RBCs added to the test tube. Addition of Coombs Reagent, then causes the sensitized Type 0 Test RBCs to agglutinate, indicating that IgG antibodies are present in the serum. REF : ananthanarayana 10th ed 