<prompt> Hybridoma used in:In situ hybridizationSequencing DNAProduction of monoclonal antibodiesProduction of continuous cell lines  <response>: ln 1975, George Kohler and Cesar Milstein created hybrid cells that will make unlimited quantities of antibodies with defined specificities, which are termed as monoclonal antibodies . This discovery know as hybridoma technology Principle - fusion between myeloma cell malignant plasma cell and spleen cell from a immunized animal. Hybridoma technology can make available highly specific antibodies in abundant amounts. use of monoclonal antibodies Example of monoclonal ab trastuzumab - EGRFHER2 INHIBITORS BEVACIZUMAB - ANGIOGENESIS INHIBITORS 