<prompt> A 5 year old girl came with history of progressively increasing pallor since bih and hepatosplenomegaly. Which of the following is the most relevant test for achieving diagnosis -Hb electrophoresisPeripheral smear examinationOsmotic fragility testBone marrow examination  <response>: Hb electrophoresis helps to confirm beta thalassemia Hb F increased ,HbA2 increased . Reference :Harsh mohab textbook of pathology sixth edition pg no 322. 