<prompt> To distinguish between primary bacterial peritonitis from secondary peritonitis the following biochemical parameters in ascitic fluid has been used, EXCEPT:Total protein 1 gdlLDH greater than the upper limit of the normal of the serumGlucose 50 mgdlPMN count 250 cellsmm3  <response>: Secondary bacterial peritonitis is the ascitic fluid infection caused by a surgically treatable intraabdominal source of infection. It can be divided into two groups 1. Those with free perforation of a viscus eg : duodenal ulcer perforation 2. Those with loculated abscess eg : perinephric abscess The characteristic ascitic fluid findings in the setting of secondary bacterial peritonitis are Ascitic fluid PMN 250 cellsmm 3 with at least two of the following criteria 1. Ascitic fluid total protein 1 gdl 2. LDH greater than the upper limit of the normal of the serum 3. Glucose 50 mgdl If Ascitic fluid carcinoembryonic antigen CEA is 5 ngmL and alkaline phosphatase 240 UL indicates gut perforation. Ref: AASLD practice guidelines: Hepatology, Vol.49, No.6 , 2009. 