<prompt> Which of the following is the primary cell line -Chick embryo fibroblastHela cellsVero cellsWI-38  <response>: Human diploid fibroblasts HDF cell line is used for cultivation of coxsackie A7,9,16; CMV; Echovirus;enteroviruses; HSV-1,2; Poliovirus: parainfluenza virus, rhinovirus and VZV.Cultivation of virusesAs viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium. Threemethods are employed for cultivation of viruses :1. Animal Inoculation Earliest method Mice are most widely employed animals Other animals used are guinea pigs, rabbits and ferrets.2. Embryonated eggs Sites of inoculation are:a Chorioallantoic Membrane --- visible pocks are produced HSV, Vaccinia, Smallpoxb Amniotic Sacc Allantoic cavity Influenza Some paramyxovirusesd Yolk Sac Some viruses Chlamydia Rickettsia3.Tissue culture Most important method for virus isolation. Three types of tissue culture are availableI. Organ CultureII Explant CultureIII. Cell Culture - routinely employedCell culture may be4. Primary cell culture Rhesus monkey kidney, human amnion, chick embryo fibroblast, human embryonic kidney.5. Diploid cell strains:- Human diploid fibroblast HL-8, WI-38.6. Continuous cell line:- Hela, HEP-2, McCoy, Vero. 