<prompt> Screening test for vitamin D deficiency is7-dehydrocholestrol1,25 dihydroxy vitamin D25 hydroxy vitamin DSerum calcium levels  <response>: Total serum 25-hydroxyvitamin D the major form of vitamin D that circulates in the body is used for screening.Recently USPSTF US preventive services task force concluded that the evidence on screening for vitamin D deficiency in asymptomatic adults to improve health outcomes is insufficient 