<prompt> Speckled pattern is seen in -Anti Sm antibodyAnti ds DNA antibodyAnti histone antibodyAnti chromatin antibody  <response>: Ans. is a i.e., Anti Sm antibody o The most commonly used method to detect antinuclear antibodies is indirect immunofluorescence. o The pattern of nuclear fluorescence suggests the type of antibody present in patients serum. o Four basic patterns are recognized :? 1. Homogenous or diffuse nuclear chromatin staining --- Reflects antihistone and antichromatin antibodies. 2. Rim or peripheral staining Anti ds DNA antibody. 3. Speckled pattern -- Most common pattern on immunofluorescence. Reflects the presence of non DNA nuclear contents -- anti Sm, anti - Ro, anti-La, anti SS-A, and anti SS-B antibodies. 4. Nucleolar pattern -- Nucleolar RNA. 