<prompt> Temperature and timing in autoclaving -121degC for 15 minutes160degC for 45 minutes190degC for 1.5 minutes134degC for 15 minutes  <response>: Ans. is a i.e., 121degC for 15 minutes MethodTemperature degCHolding time in minutesAutoclave12112613415103Hot air oven16017018019045187-5T5 