<prompt> Investigations in a clinically suspected case of tuberculosisMantoux in childrenSputum AFBPCRAll  <response>: The diagnostic procedures in a clinically suspected case of TB include AFB microscopy of sputum using Ziehl-Neelsen basic fuschsin stain;Mycobacterium culture using Lowenstein-Jensen or Middlebrook medium;nucleic acid amplifications using PCR,BACTEC;radiographic procedures like chest X ray with classic picture of upper-lobe disease with infiltrates cavities,CT;Tuberculin skin testing with purified protein derivative which is a screening for latent M.tuberculosis infection Reference:Harrisons Medicine-18th edition,page no:1350,1351. 