<prompt> Which of the following enzymes are elevated in cholestasis?Alkaline phosphatase5 nucleotidaseGamma glutamyl transpeptidaseAll the above  <response>: Ans. is d i.e., All the above Enzymes in cholestasis The activities of three enzymes-- alkaline phosphatase, 5-nucleotidase, and y-glutamyl transpeptidase GGT--are usually elevated in cholestasis. Alkaline phosphatase and 5-nucleotidase are found in or near the bile canalicular membrane of hepatocytes, while GGT is located in the endoplasmic reticulum and in bile duct epithelial cells. Reflecting its more diffuse localization in the liver, GGT elevation in serum is less specific for cholestasis than are elevations of alkaline phosphatase or 5-nucleotidase. 