<prompt> Shigellosis is best diagnosed by:-Stool examination cultureSerologySigmoidoscopyEnzyme  <response>: Shigellosis is best diagnosed by Stool examination culture. Option 1 1. Stool is the specimen of choice. Diagnosis of shigellosis is made by isolating Shigella spp. from feces. Fresh feces are inoculated without delay or transpoed in a suitable medium, such as Sachs buffered glycerol saline. Also, rectal swabs may be taken from the site of ulcer by sigmoidoscopy. Option 3 - Sigmoidoscopy: It can reveal the extent of the disease. Option 4 - For diagnosis of Shigellosis: we dont detect any enzyme. 