<prompt> In alpha-thalassemia trait, electrophoresis shows:Increased HbF normal HbA2Normal HbF normal HbA2Normal HbF decreased HbA2Decreased HbF normal HbA2  <response>: Electrophoresis is a screening test for hemoglobinopathies Normal individual Sickle cell anaemia a thalassemia b thalassemia HbA 95 Absent Less HbF 2 Present Normal Increased HbA2 3 Present Normal Increased Note: In sickle cell anemia absence of HbA means the disease severity is very high. But in sickle cell trait HBA is present. Note: Screening test for both a b thalassemia is NESTROF IOC for both a b thalassemia is HPLC 