<prompt> Viruses can be isolated from clinical samples by cultivation in the following except -Tissue cultureEmbryonated eggsAnimalsChemically defined media  <response>: Cultivation of viruses As viruses are obligate intracellular parasites they cannot be grown on any inanimate culture medium. Three methods are employed for cultivation of viruses :1. Animal InoculationEarliest methodMice are most widely employed animalsOther animals used are guinea pigs, rabbits and ferrets.2. Embryonated eggsSites of inoculation are :Chorioallantoic Membrane --- visible pocks are produced HSV, Vaccinia, SmallpoxAmniotic SacAllantoic Cavity InfluenzaSome paramyxovirusesYolk SacSome virusesChlamydiaRickettsia3. Tissue cultureMost important method for virus isolation. Three types of tissue culture are availableOrgan CultureExplant CultureCell Culture - routinely employed 