<prompt> Which of the following agents are used to check sufficiency of a. Autoclaving b. Hydroclaving c. Microwavinga - Bacillus atrophaeus , b - Bacillus subtilis , c - Geobacillus stearothermophilusa - Bacillus subtilis , b - Bacillus atrophaeus , c - Geobacillus stearothermophilusa - Geobacillus stearothermophilus , b - Bacillus subtilis , c - Bacillus atrophaeusa - Bacillus atrophaeus , b - Geobacillus stearothermophilus , c - Bacillus subtilis  <response>: Thermal methods Principle Agent used to check its sufficiency 1. Autoclaving - Steam under high temperature - Temperature used in India 121oC, 45 min ; 15 psi Geobacillus stearothermophilus 2. Hydroclaving - Steam under pressure - Temperature used 121oC or 132oC Bacillus subtilis 3. Microwaving - Generation of convention current in heated water molecules - Frequency of 2450 MHz wave length of 12.24 nm is used. Bacillus atrophaeus 