<prompt> Volume of distribution is NOT affected by all EXCEPTBinding to plasma proteinDrug clearanceLipid insolubilityAbsence of blood brain barrier  <response>: Binding to plasma protein 31-33-KDT 6th The extent of distribution of drug depends on Lipid solubility Ionization at physiological pH dependent on pKa The extent of binding to plasma and tissue proteins Differences in regional bloodflow 