<prompt> Steps of PCR in sequence are?Extend DNA, anneal primers, Denture DNAAnneal primers, extend DNA, Denature DNADenature DNA, anneal primers, Extends DNADenature DNA, Extend DNA, Anneal primers  <response>: PCR - method of amplifying a target sequence of DNA Developed by Karry Mullis in 1984 Essential requirement - 1 targeted DNA 2 Two primers synthetic oligonucleotides of 17-30 nucleotides length that are complementary to target DNA.3 Four deoxyribonucleotide4 THERMOSTABLE DNA polymerase Each cycle has three step 1 Denaturation - The DNA sample is first heat denatured 90degC to separate the two strands of the template DNA containing the target sequence2 Renaturation or annealing -the primers, added in excess, are allowed to anneal to the DNA typically at 50-75degC in order to generate the required template-primer complex.3 Synthesis extension -he initiation of DNA synthesis occurs at 3-hydroxyl end of each primer. The primers are extended by joining the bases complementary to DNA strands Each cycle of PCR takes about 3-5 minutes Three stage of each cycle in relation to temperature and time 