<prompt> Which of the following enzyme does not catalyse reversible step in glycolysis?PFKEnolasePhosphoglyceromutaseGlyceraldehyde-3-phosphate dehydrogenase  <response>: Enzymes of glycolysis - a process which converts 6 carbon glucose to 3 carbon unit pyruvate lactate - are present in cytoplasm. Whereas, complete oxidation of glucose to CO2 H2O requires mitochondrial enzymes of TCA cycle.Out of total 9 enzymes used in glycolysis,3 enzymes - hexokinase, phosphofructokinase PFK-1 and pyruvate kinase are used in irreversible steps.6 enzymes - phosphohexose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase, 1, 3-bisphosphoglycerate kinase, phosphoglyceromutase and enolase are used in reversible steps.2 enzymes used in energy utilizing steps are - hexokinase using 1 ATP and phosphofructokinase PFK-1 using 1 ATP. This energy consumption is for 1 molecule of glucose.3 enzymes used in energy producing steps are - glyceraldehyde-3-phosphate dehydrogenase producing 2 NADH 5 ATP, 1, 3-bisphosphoglycerate kinase producing 2 ATP and pyruvate kinase producing 2 ATP. This energy production is for 1 molecule of glucose or 2 molecules of glyceraldehyde-3-phosphate. 