<prompt> What does the term nick translation refer to?Translation of RNA into polypeptideRegulation of gene expressionTechnique to label DNATechnique to digest DNA  <response>: A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5 to 3 direction. This propey is used to incorporate radio labeled nucleotides in DNA. 