<prompt> Insulin causes decrease in activity of which enzyme-PFK-1GlucokinasePyruvate CarboxylaseAcetyl CoA Carboxylase  <response>: Ans. is c i.e., Pyruvate carboxylase Effect of insulin on major metabolic pathway effects of carbohydrate feedingPathwayEffectRegulated enzymeCarbohydrate metabolism o Glycolysis Phosphofructokinase-1, Pyruvate kinase Pyruvate dehydrogenase Gluconeogenesis Pyruvate carboxylase, PEP carboxykinase, fructose-1,6-bisphophatase glucose-6-phosphotaseo Glycogenesis glucokinase, glycogen synthaseo Glycogenolysis glycogen phosphorylaseLipid metabolism o Lipogenesis Acetyl-Co carboxylase, Fatty acid synthaseo Lipolysis Hormone sensitive lipaseo Cholesterol Synthesis HMG-CoA reductaseo Triacylglycerol Synthesis Acyl-CoA-glycerol-3-phosphate transferase, glycerol kinaseo Lipoprotein degradation Protein metabolism Lipoprotein lipaseo Protein synthesis RNA polymerase and ribusome assembelyNote:- Pyruvate dehydrogenase is neither a glycolytic nor a TCA cycle enzyme, it acts as a bridge between the two. 