<prompt> What is involved in formation of d-TMP from d-UMP?N5, N10-methylene tetra hydrofolateForm imino folateN5 formyl folateDihydro folate  <response>: A i.e. N5 - N10 methylene tetrahydrofolated-UMP deoxy-uridine monophosphate is conveed to d-TMP deoxy-thymidine monophosphate which uses N5, N10 -methylene tetra hydrofolate as a source of methyl group 1 carbon 2 hydrogenQ.. This is an unusual reaction in that THF tetrahydrofolate contributes not only 1 carbon unit but also 2 hydrogen atoms from pteridine ring , which causes oxidation of THF to DHF. Disorders of folate vitamin B12 metabolism cause deficiency of TMP.Thymine analog, antitumor drug 5-flurouracil 5Fu is conveed to 5-F dUMP, which becomes permanently bound to the inactive thymidylate synthaseQ. So 5FU is a suicide inhibitor. Methotrexate Mtx and antibacterial trimethoprim inhibits dihydrofolate reductase, an enzyme which reduces DHF to THFQ. 