<prompt> True about the Sickle cell disease are all, Except -Single nucleotide change results in change of Glutamate to ValineSticky patch is generated as a result of replacement of a non polar residue with a polar residueHbS confers resistance against malaria in heterozygotesRFLP results from a single base change  <response>: Sticky patch in sickle cell disease results from replacement of a polar residue Glutamate with a nonpolar residue Valine at the 6th position of the beta chain.Sickle cell anemia is caused by a point mutation, i.e. mutation of just one single nucleotide of DNA. The altered codon specifies a different amino acid glutamic acid, and this causes a structural abnormality of the β-globin molecule.Heterozygotes for the sickle cell mutation are more resistant to malarial infection because the erythrocytes of heterozygotes provides less favorable environment for plasmodium parasite.RFLPs result from single-base changes e.g. sickle cell disease or from deletion or insertions of DNA into a restriction fragment e.g. thalassemias. 