<prompt> Screening method for medullary carcinoma thyroid isS. alkaline phosphateS. calciumSerum calcitoninS. acid phosphatase  <response>: MCT can be of two types - sporadic and familial occurring in MEN syn. 2A 2B and Familial cases without MEN syn. The familial MTC are more aggressive, multicentric bilateral and present at a younger age, therefore high risk cases should be screened for MTC by - detecting RET mutation. The genetic screening tests are supplemented by estimating serum calcitonin levels in the basal state and after stimulation by either calcium or pentagastrin. Prophylactic total thyroidectomy is indicated in RET mutation carriers once the mutation is confirmed. Ref : Bailey love 25e p797 