<prompt> Result of storage of packed RBCsOxy-hemoglobin curve shift to rightDecrease in 2-3DPGIncrease in pHDecrease in lactate  <response>: Answer: b Decrease in 2-3DPG. 2, 3-BPG is essential for the normal oxygen transport function of hemoglobin. However, storing blood in the currently available media results in a decrease in 2, 3-PBG. Stored blood displays an abnormally high oxygen affinity, and fails to unload its bound oxygen properly in the tissues. Hemoglobin deficient in 2,3-BPG thus acts as an oxygen trap rather than as an oxygen transport system. Transfused RBCs are able to restore their depleted supplies of 2,3-BPG in 6-24 hours. However, severely ill patients may be compromised if transfused with large quantities of such 2, 3-BPG-stripped blood. Note: The maximum storage time for red cells has been doubled 21 to 42 days, with median time of 15 days by changes in H, phosphate and hexose sugar concentration, and by the addition of adenine. Although the content of 2,3-BPG was not greatly affected by these changes, ATP production was increased and improved RBC survival. Transfusion of large volumes of stored blood, which has a low level of 2,3-BPG can lead to sudden hypoxia, since it can cause a left-shifted ODC. 