<prompt> Which one of the following statements about Km value is true?Km is substrate concentration at maximal velocityNumerical value of km is propoional to affinity of enzyme for substrateKm is independent of enzyme concentrationKm denotes that 100 of enzyme molecules are bound with substrat molecules at that concentration  <response>: Substrate Concentration, A small Km indicates that the enzyme requires only a small amount of substrate to become saturated. Hence, the maximum velocity is reached at relatively low substrate concentrations. A large Km indicates the need for high substrate concentrations to achieve maximum reaction velocity. 