<prompt> All the following can be used to detect the mutation, exceptSingle strand conformational polymorphismLigase chain reactionPolymerase chain reactionDNA Sequencing  <response>: Methods used for detecting mutations Cytogenetics analysis FISH PCR, RFLP DNA sequencing, Southern blotting Microarrays Single-stranded conformational polymorphism SSCPRNAase cleavageDenaturating gradient gel electrophoresis DGGEProtein truncation test 