<prompt> RFLP is used forAnalysis of chromosomal structuresDNA estimationSynthesis of nucleic acidDetecting proteins in a cell  <response>: Reastiction fragment length polymophism RFLP : A polymorphic gene is one ,in which the variant alleles are commonin more than 1 of the total population. The existence of two or more types of restriction fragment patterns is called restiction fragment length polymorphism RFLP. This can be used as a genetic marker. DNA is treated with restriction enzymes, which cleave DNA into fragments of lengths. The electrophoresis is done in agarose gels, when the fragments are seperated. Finally, the DNA from the agarose gel is transferred on to nitrocellulose paper southern blottingand hybridized with labeled probe sequences. Genotypic changescan be recognized by the altered restriction fragments. REF : DM VASUDEVAN TEXTBOOK ,7th EDITION , Page no no :637. 