<prompt> Sulphur granules are seen in -Sulphur poisoningActinomycosisH2SO4 poisoningStaphylococcal infection  <response>: Ans. is b i.e., Actinomycosis Laboratory diagnosis of actinomycosis The diagnosis is most commonly made by microscopic identification of sulphur granules an in-vivo matrix of bacteria calcium phosphate, and host material. The sulfur granules are bacterial colonies and will be found to consist of a dense network of thin Gram positive filaments, surrounded by a peripheral zone of swollen radiating club shaped structures, presenting a sun ray appearance. The clubs are believed to be antigen - antibody complexes. Colonies resemble a molor tooth. Granules of actinomyces are yellow colored. Diagnosis can also be made by isolation of actinomyces in culture. The culture media used are : Thioglycollate liquid medium Brain heart infusion agar In thioglycollate: A. bovis produces general turbidity A. israelic grows as fluffy balls at the bottom of the tube. On solid media : A. isralii produces small spidery colonies. A. isralii produces colonies which give a bread crumb appearance. 