<prompt> The effect of chilling in refrigeration analgesia includesInterference with conduction of nerve impulseReduction of metabolic rate and oxygen requirementInhibition of bacterial growth and infectionAll of the above  <response>: For chilling analgesia cryoanalgesia CO2 or N2O is used. At such low temperatures there is inhibition of nerve conduction producing analgesia. Hypothermia decreases metabolic rate and oxygen consumption of tissue. Hypothermia inhibits the bacterial growth. Healing is also retarded at low temperatures. 