<prompt> Normal saline is used as diluent in which vaccine-MeaslesRubellaBCGHAV  <response>: Ans. is c i.e., BCG BCG vaccineo BCG vaccine is a live attenuated vaccine produced by Bacille Calmette Guerin an avirulent strain produced by 230 subcultures over a period of 13 years,o Types of vaccine - It is a live attenuated vaccine. There are two types of vaccineLiquid fresh vaccineFreeze dried lyophilized - more stable, currently in use.o Diluent - Normal saline is recommended as a diluent for reconstituting vaccine, as distilled water may cause irritation. The reconstituted vaccine should be used within 3 hours.o Route - Intradermalo Site - Just above the insertion of deltoid usually lefto Dosage - Usual strength is 0.1 mg in 0.1 ml For newborn 4 weeks, 0.05 ml.o Age of administraton - Either at birth or at 6 weeks of age simultaneous with DPT and polio,o Storage - BCG vaccines are stable for several weeks at ambient temprature in tropical climate, and for upto 1 year. If kept away from direct light and stored in a cool environment. The vaccine must be protected from exposure to light during storage wrapped up in double layer of red or black cloth,o Duration of protection - 15 to 20 years,o Booster - Not advised under EPL 