<prompt> Virus can be isolated from clinical samples by cultivation in the following except:Tissue cultureEmbryonated eggsAnimalsChemically defined media  <response>: Ans. d Chemically defined media Ref Ananthanarayan 8e 450, 9e, p 451 Isolation of virus Method of isolation consists of: Inoculation into animals, eggs or tissue culture after the processed to remove bacterial contaminants. Ananthanarayan 8e, p 450, 9e, p 451 As many viruses adenoviruses, enteroviruses are frequently found in normal individuals, so only recovery of viral agent from patient doesnt prove that it is the causative agent of the patient illness. Organism not grown in aificial cultural media are: Chlamydia Rickettsia Rhinosporidium Pathogenic treponemes M. leprae Pneumocystis Viruses. 