<prompt> Which organism cannot be cultured in cell free media -Klebsiella rhinoscleromatisKlebsiella ozaenaeTreponema pallidumAll  <response>: Ans. is c i.e., Treponema pallidum . Treponema pallidum is an obligate intracellular organism. Thus it cannot be cultivated in cell free medium. . Pneumocystis Jiroveci and Rhinosporidium seeberi can not be cultivated. 