<prompt> Long acting insulin:LisproAspartGlargineZn suspension of insulin  <response>: Ans. C. GlargineRef: Essentials of Medical Pharmacology by KD Tripathi 7thled, p263ExplanationLente insulin is a combination of 30 semilente amorphous and 70 ultralente crystalline insulin zinc suspension. One of the problems with regular insulin is it peaks 2 to 3 hours after administration and sustains over 6 to 8 hours, which does not mimic the physiological insulin response. This leads to postprandial hyperglycemia and intermittent hypoglycemia. To overcome this, long-acting modified or retard preparations of insulin were developed. Retard preparations, are obtained by making insulin insoluble either by: Complexing it with protamine isophane or By precipitating it with excess zinc and increasing the particle size ultralente, semilente, lente.Insulin-zinc suspensionIsophane Neutral Protamine Hagedorn or NPH insulinTwo types of insulin-zinc suspensions have been produced Protamine is added in a quantity just sufficient to complex all insulin molecules Neither of the two is present in free form On S.C. injection, the complex dissociates slowly to yield an intermediate duration of action Mostly used in combination with regular insulin 70:30 or 50:501. Ultralente Large particles Crystalline Long-acting Insoluble in water2. Semilente Smaller particles Amorphous Short acting Lente ultralente semilente in the ratio of 7:3 It can be remembered like this: 70 bigger particle and 30 smaller particle Therefore lente insulin which is 70 ultralente 30 semilente can be written in different ways as follows: 70 large particle 30 small particle 70 crystalline 30 amorphous Answer 70 long acting 30 short acting Apart from the above mentioned retard preparations, recently other insulin analogs like rapidly acting lispro, aspart, glulisine peakless and long-acting glargine and detemir have become available.TypeExampleRapid actingLisproAspartGlulisineShort actingRegular soluble insulinIntermediate actingLenteNPH or isophaneLong actingInsulin glargineInsulin detemir 