<prompt> Direct Coombs test is positive for:IgAIgEIgMIgG  <response>: Autoimmune hemolytic anaemia in which the red cell injury is brought about by auto-antibodies reacting with antigens present on red cell membrane. Antiglobulin test direct Coombs test is employed to detect the antibody on red cell surface.Key Point:DAT Direct Antiglobulin Test testing typically involves the use of a polyspecific reagent consisting of IgG and complement C3.Mohan H. Textbook of pathology. Jaypee Brothers Medical Publishers; 2015. Edition 7. Page 60 