<prompt> Which of the following is non cultiviable invivoMycobacterium aviumHIVMycoplasma pneumoniaeHepatitis-C virus  <response>: i.e. Hepatitis C- Virus : 432-35-Ananthanarayan 8th Explanation : Some viruses are not culturable in vitro due to ack of susceptible cell lines, e.g. certain blood- borne e.g. Hepatitis C virus, HCV and water -borne e.g. Norwalk virus viruses.MYCOPLASMAMycoplasma - smallest free living organism known are prokaryotes that are bounded only by a plasma membrane Their lack of a cell wall is associated with cellular pleomorphism and resistance to cell wall active antimicrobial agents such as penicilline and cephalosporins beta lactams, vancomycin, Bacitracin The organism small genome limits biosynthesis and explains the difficulties encountered with in vitro cultivation 1068-H17th Myeo, pneumoniae can be grown on artificial media, but the process is exacting, requires special media and takes more than 2 weeks. Thus mycoplasma cultures do not provide any timely information Mycoplasma are bounded by a triple layered unit membrane that contains s sterol therefore when mycoplasma are grown in culture media, the media required for the growth of the organsm should be rich in sterol This is provided by adding 20 horse or human serum 