<prompt> Which of the following is true for phosphofructokinaseIt uses fructose 1, 6 bisphosphate as substrateIt generates ATPIt catalyses an irreversible reaction in glycolysisIt is activated by increased levels of ATP and citrate  <response>: Phosphofructokinase catalyses the first committed step of glycolysis. It is the rate limiting enzyme of glycolysis. The reaction is irreversible. 