<prompt> Under National Poliomyelitis Elimination Programme, Poliomyelitis is diagnosed by:-Antibodies titre rise in bloodViral isolation in stoolViral microscopy in stoolClinical examination  <response>: Polio virus diagnosis in India: Is done by Viral culture and isolation from Stool Samples collected from cases of Acute Flaccid Paralysis AFP Previously it was done through Antibody titre rise. 2 stool samples bw 24-48hrs each weighing 8gm is taken sent it to lab through reverse cold chain. 