<prompt> Which is the best temperature for putrefaction?- 10 degrees C0 degrees C30 degrees C50 degrees C  <response>: Ans. is c i.e., 30 degrees C Putrefaction is the last stage in resolution of body from the inorganic to organic state and is a certain sign of death. The putrefaction is due to:i Autolysis: The enzymes of the body, break down the dead body causing proteolysis, lipolysis and glycolysis.ii Bacterial enzymes: Aerobic and anaerobic bacteria present in small intestine e.g. C. welchii, staphylococcus, E.coli etc. release enzymes especilly lipase and lecithinase, which act on body to cause breakdown. Three conditions are necessory for putrefaction : i warmth 10-45deg, ii moisture humidity and iii air. If air is absent, adipocere formation saponification occurs, and in the absence of moisture, mummification occurs. 