<prompt> A method of sterilization that involves exposure to 100degC for 20 minutes on 3 successive days is known as ___AutoclavingTyndallizationPasteurisationInspissation  <response>: 1 Inspissation fractional sterilization 80 to 85degC for 30 min for 3 days by Inspissator For media containing egg or serum - LJ media, Dorsets egg medium, Loefflers serum slope 2. Tyndallisation Intermittent sterilization:100degC, 20 min, 3 consecutive days. Nutrient media and media containing sugars or gelatin 1st day all vegetative bacteria are killed. On II and III day spores that germinate are killed. 3. Autoclave -121 oC for 15 min with 15 lbs. pressure This type of sterilization is done by steam heat under pressure. Use: Glass ware, surgical dressing, culture media, rubber, gloves, surgical instrument are sterilized by this method Autoclaved Sterilization control: Spores of Geobacillusstearothermophilus Bacillus stearothermophilus, Thermocouples, Brownes tube, Autoclave tapes. 4 Pasturization-used for disinfection of milk holder method: moist heat oC for 30 minutes Flash method :moist heat oC for 15 -20 seconds followed by rapid cooling to the temperature of 13oC UHT method: moist heat 149 oC for 0.5 sec 