<prompt> Effective red cell diameterDifferent in males and femalesDiameter of 500 micronsThalassemia minor anemia may present with normal RBC diameterMixed iron and folic deficiency anemia produce microcytic  <response>: Answer: C Thalassemia minor anemia may present with normal RBC diameter 265-68-Hrshmohon 7th 630 Rabbins- basis diseaseRed blood cell distribution width RDW or RCDW is a measure of the variation of red blood cell RBC volume that is reported as part of a standard complete blood count. Usually red blood cells are a standard size of about 6-8 pm in diameter. Certain disorders, however, cause a significant variation in cell size. Higher RDW values indicate greater variation in size. Normal reference range in human red blood cells is 11.5-14.5. If anemia is observed, RDW test results are often used together with mean corpuscular volume MCV results to determine the possible causes of the anemia. It is mainly used to differentiate an anemia of mixed causes from an anemia of a single cause. Deficiencies of Vitamin Bl2 orfolate produce a macrocytic anemia large cell anemia in which the RDW is elevated in roughly two- thirds of all cases; however, a varied size distribution of red blood cells is a hallmark of iron deficiency anemia, and as such shows an increased RDW in virtually all cases. In the case of a mixed iron and B!2 deficiency, there will normally be a mix of both large cells and small cells, causing the RDW to be elevated. An elevated RDW red blood cells of unequal sizes is known as anisocytosis. An elevation in the RDW is not characteristic of all anemias; anemia of chronic disease,hereditary spherocytosis, acute blood loss, aplastic anemia anemia resulting from an inability of the bone marrow to produce red blood cells, and certain hereditary hemoglobinopathies including some cases of thalassemia minor all may present with a normal RDW The human erythrocyte is a biconcave disc, 7-2 pm in diameter, and has a thickness of 2-4 pm at the periphery and 1 mm in the centre More than 90 of the weight of erythrocytes consists of haemoglobin. The life span of red cell is 120 - 30 day. Range of normal red cell count in health in 5.5 - 1.0 x1012L in men and 4.8 - l.0x 1012l in women Packed cell volume PCV or haematocrit- 0.47 0.07LL 40-54 in men- 0.42 - 0.05 LL 37-47 in women Reticulocyte count 0.5-l .5 Red cell distribution width-11.5-14.51. Mean corpuscular volume MCV PCV in LLRBC CountL Normal value is 85 - 8fl 77-93 fl2. Mean corpuscular haemoglobin MCH HbLRBC CountL Normal range is 29.5 2.5pg 27-32 Pg3. Mean corpuscular haemoglobin concentration MCHC Hb dlPCV in LLNormal value is 32.5 2.5 gdl 30-35 gdl The size of the RBC is measured by MCV. MCHC is independent of red cell count and size Monocyte is the largest mature leucocyte in peripheral blood Flow cytometry is done on - Lymphocytes The anticoagulant used in Wintrobe ESR- Double oxalate1. Alkaline phosphatase is specific to Neutrophils cells2. Acid phasphatase is found in all hematopoitic cells but the highest levels are found in macrophages and osteoclasts Monocytes Anticoagalant of choice for coagulation test is 3.2 sodium citrate Potassium oxalate sodium fluoride is anticoagulant used in collection of blood sugar.Increases Leucocyte Alkaline phosphateDecreased Leucocyte Alkaline phosphate Infections Leukamoid reactions Polycythemia- vera Paroxysmal nocturnal hemoglobinuria PNH CMLBlood group A is associated with1. Carcinoma stomach, cervix salivary gland pancreas2. Pernicious anemia3. Thrombosis in OCP users 