<prompt> Sterilization of culture media containing serum is by :AutoclavingMicropore filterGamma radiationCentrifugation  <response>: Most of the culture media are sterilized by Autoclaving at 121C for 15 min at 15 lb pressure inch2Constituents like serum, trypsin, growth factors, proteins, amino acids, vitamins, hormones, carbohydrates and plant extracts are thermolabile and may decompose during autoclaving. Therefore, these must be sterilised by filtration. 