<prompt> SYBR Green Dye is used for:HPLCImmuno-fluorescencePCRELISA  <response>: Dyes used in real time PCR SYBR based detection TaqMan based detection Uses SYBR Green dye ds-DNA binding dye Not sequence specific Uses a fluorogenic probe specific to target gene It accumulates during PCR. Real Time PCR Quantitative Fluorescence PCR QF-PCR q PCR: Products are detected by fluorescence during NOT after the PCR reaction in real time, not end time PCR. 