<prompt> Which of the following is used for isolation fo chlamydia -MicroimmunofluoroscenceYolk sac ioculationELISAImmunofluroscence  <response>: Ans. is b i.e., Yolk sac Isolation of chlammydia . Chlamydiae are obligate intracellular parsite, therefore cannot be cultured on nonliving aificial media cell free media. . Isolation of chalamydia can be done by inoculation into :? Embryonated eggs paicularly in yolk. Experimental animals Tissue culture cell culture, e.g., McCoy cells, HeLa cells. . Amongst these, cell culture is the preferred method of isolation. 