<prompt> In Anaerobic glycolysis, there is gain of -2 ATP 2 NAD2 ATP2 ATP 2 NADH4 ATP 2 FADH2  <response>: Net gain in anaerobic glycolysis is only - 2 ATP. - 2 NADH are produced also Glyceraldehyde-3-P dehydrogenase step - But 2 NADH are used also Lactate dehydrogenase step. Similarly - 2 NAD are produced also Lactate dehydrogenase step - But 2 NAD are used also Glyceraldehyde-3-P dehydrogenase step. So there is no net gain of NADH or NAD. 