<prompt> Eosin-5-Maleamide flow cytometry is used for diagnosis of:G6PDHereditary spherocytosisSickle cell anemiaAlpha thalassemia  <response>: Ans: b Hereditary spherocytosisRef: Wintrobes Hematology, compiledHereditary SpherocytosisAD inheritanceMost common mutation - AnkyrinMost severe mutation SpectrinThe spherocytes of HS have 2 defects:Reduced deformabilityReduced membrane plasticityPeripheral smearSpherocytesHowell jolly bodies - post splenectomyHemolysis - ExtravascularDiagnosisPeripheral smearOsmotic fragility test - ScreeningGold standard - Flow cytometry - EMA Eosin-5-Maleamide Flowcytometry EMA FlowcytometryA flowcytometric test based on eosin-5-maleamide EMA which binds to band 3 of red cell membrane is a sensitive and specific test for diagnosis of HS.The mean fluorescence intensity of EMA tagged red cells is lower in HS MCF0.80.EMA fluorescence is normal or increased in AIHA DD for spherocytosis. 