<prompt> Single base pair substitution is related to which of the following?RFLPPCRFISHSouthern blot  <response>: The term RFLP Restriction Fragment Length Polymorphisms refers to single base pair substitutions in germ line DNA of different individuals that either destroys or creates new recognition site for a given restriction enzyme. PCR is used to amplify a specific region of a DNA strand the DNA target. FISH is atechnique used to identify the presence of specific chromosomes or chromosomal regions through hybridization attachment of fluorescently-labeled DNA probes to denatured chromosomal DNA. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization. 