<prompt> During unwinding of DNA, relief of supercoil is made possible byTopoisomeraseGyraseHelicasePolymerase  <response>: During unwinding of DNA, relief of supercoil is made possible by Topoisomerase DNA helicase unwinds the DNA without cutting but using energy from hydrolysis of ATP. Gyrase is a bacterial type II topoisomerase which acts on closed circular DNA bacteria have circular DNA. 