<prompt> The peripheral blood smear of a patient shows features of thalassemia, also presented with anemia. Family history is also ve. The investigation was done to establish the diagnosis is -ESR estimationBlood spherocyte estimationBone marrow aspirationHb-electrophoresis  <response>: Hemoglobin electrophoresis should always be the first investigation to includeexclude the diagnosis of thalassemia.The level of normal adult hemoglobin HbA is markedly decreased with proportionate increase in HbA2 and HbF.X-ray findings in thalassemia1. Crew cut appearance2. Hair on end appearance 