<prompt> Various biomedical tools are used for DNA technology. Which test uses oligomer with single base pair substitution?PCRRFLPError coded mutation analysisFISH  <response>: An inherited difference in the pattern of restriction enzyme digestion eg, a DNA variation occurring in more than 1 of the general population is known as a restriction fragment length polymorphism RFLP. RFLPs result from single-base changes eg, sickle-cell disease or from deletions or inseions CNVs of DNA into a restriction fragment eg, the thalassemias and have proved to be useful diagnostic tools. Ref: Weil P. 2011. Chapter 39. Molecular Genetics, Recombinant DNA, Genomic Technology. In D.A. Bender, K.M. Botham, P.A. Weil, P.J. Kennelly, R.K. Murray, V.W. Rodwell Eds, Harpers Illustrated Biochemistry, 29e. 