<prompt> A 45-year-old female complains of lower abdominal pain and vaginal discharge. On examination, there is cervicitis along with a mucopurulent cervical discharge. The best approach to isolate the possible causative agent would beCulture chocolate agar supplemented with HaeminCulture on McCoy cellsCulture on a bilayer human blood agarCulture on vero cell lines  <response>: Sexually transmitted diseases may be present in the following ways: Vaginal discharge, Cervicalurethral discharge due to Neisseria gonorrhoea or Chlamydia trachomatis Genital ulcers etc. The culture for sexually transmitted diseases is done by Irradiated McCoy cells medium Ref: Ananthanarayan and Paniker;s Textbook of Microbiology, 9th edition; Pg no 675 