<prompt> Triple iron sugar medium contains all, except ?LactoseSucroseGlucoseMaltose  <response>: Ans. is d i.e., Maltose Triple sugar iron agar is used for the differentiation of microorganisms on the basis of :? Fermentation of dextrose glucose, lactose and sucrose. Production of H2S. It is recommended for differentiation of enteric gram negative bacilli from clinical specimens, dairy samples and food products. Contents of the medium are :? Enzymatic digest of casein and animal tissue. Yeast enriched peptone. Three sugars : Dextrose glucose, lactose and sucrose. Ferric ammonium citrate. Sodium chloride. Sodium thiosulfate. Phenol red. Agar. When carbohydrates are fermented, acid production is detected by the phenol red indicator, which is yellow in acid and red in alkaline conditions. Sodium thiosulfate is reduced to hydrogen sulfide H2S and H2S reacts with an iron salt yielding typical black iron sulfide. Ferric ammonium citrate is hydrogen sulfide indicator. Results Two areas of tube are examined : i Butt of the tube, and ii Slant of the tube. An alkaline slant red- acid butt yellow indicates fermentation of dextrose glucose only - red yellow. Examples are Proteus mirabilis, salmonella thyphimurium and shigella flexneri. An acid slant yellow - acid butt yellow indicates fermentations of dextrose glucose, lactose andor sucrose - yellowyellow. Example is E coli. An alkaline slant red - alkaline butt red indicates no fermentation non-fermenter - redred. Example is Pseudomonas aeruginosa. An alkaline precipitate in butt indicates H2S production. It is produced by Proteus mirabilis and Salmonella typhimurium. 