<prompt> Nitroblue tetrazolium test is used forPhagocytesComplementT cellB cell  <response>: Ref, Harrison pg 76 In immunohistochemistry the alkaline phosphatase is often used as a marker, conjugated to an antibody. The colored product can either be of the NBTBCIP reaction reveals where the antibody is bound, or can be used in immunofluorescence. The NBTBCIP reaction is also used for colorimetricspectrophotometric activity assays of oxidoreductases. One application is in activity stainsin gel electrophoresis, such as with the mitochondrial electron transpo chain complexes. Nitro blue tetrazolium is used in a diagnostic test,paicularly for chronic granulomatous disease and other diseases of phagocyte function. When there is an NADPH oxidase defect, the phagocyte is unable to make reactive oxygen species or radicals required for bacterial killing. As a result, bacteria may thrive within the phagocyte. The higher the blue score, the better the cell is at producing reactive oxygen species. 