<prompt> Indirect Coombs test detects:Antibodies attached to RBC SurfaceAntibodies in the serumAntigens attached to RBC SurfaceAntigens in the serum  <response>: Answer is B Antibodies in the serum Indirect Coombs test detects IgG antibodies in the serum e.g. Anti-D Antibodies. Direct Coombs test detects IgG Antibodies or complements attached to the surface of RBCs. Direct Antihuman Globulin Test Detects RBCs sensitized with IgG or ComplementsC3B or C3d DAT; Coombs; Direct Coombs Detects IgG Antibodies or complements attached to the surface of RBCs. Indirect Antihuman Globulin Test Direct Coombs Test In the Direct Coombs test, red blood cells RBCs sensitized with IgG antibodies or C3b, C3d are agglutinated when Coombs reagent Rabbit Anti-IgG antibody is added to the test tube Detects Antibodies in the Serum Indirect Coombs Test In the Indirect Coombs test IgG antibodies e.g. Anti-D in the serum must first bind to blood group Type 0 Test RBCs added to the test tube. Addition of Coombs Reagent, then causes the sensitized Type 0 Test RBCs to agglutinate, indicating that IgG antibodies are present in the serum. 