<prompt> Neostigmine antagonizes nondepolarizing blockade by all of the following mechanisms, except ?Decreasing the breakdown of Acetylcholine at the motor end platePreventing K efflux from the cellIncreasing the release of Acetylcholine at the motor end plateDepolarization of the motor end plate  <response>: Ans. is c i.e., Increasing the release of Acetylcholine at the motor end palate o Anticholinestrases act by inhibiting the action of acetylcholinesterase an enzyme that degrades acetylcholine by causing its hydrolysis. o Anticholinesterases thus increase the level of acetylcholine at the neuromuscular junction. o Neostigmine also has some additional direct action on cholinergic receptors i.e., it depolarizes motor end plate. o It does not increase the release of ACH. Accumulated ACH acts on prejunctional muscarinic autoreceptors and inhibits the release of ACH. 