<prompt> The enzyme used to flush the sticky ends of DNA isKlenow fragmentPolynucleotide kinaseAlkaline phosphatasePrimase  <response>: Flushing of sticky ends means to remove the sticky ends 3 overhangs for producing blunt-ended DNA no overhangs. The sticky ends has 3 overhangs which can be digested with Klenow fragment has 3-5 exonuclease activity to produce blunt-end DNA. Tool Role Type II restriction enzymes Cleaves DNA at specific sites DNA ligase Phosphodiester bond formation between 2 fragments Klenow fragments DNA polymerase I without 5 to 3 exonuclease activity Used previously for polymerization in PCR reactions Polynucleotide kinase Catalyse the transfer of g phosphate from ATP Alkaline phosphatase Removes the phosphate group from 5 end to prevent self annealing of sticky ends of DNA Reverse transcriptase RNA dependent DNA polymerase for cDNA synthesis RNAase H Removes RNA from DNA-RNA hybrid Exonuclease III Removes nucleotide from 3 end Phage g exonuclease Removes nucleotide from 5 end Terminal nucleotidyl transferase In homopolymer tailing to conve blind end to sticky end 