<prompt> Diagnosis of thalassaemia major is based on the detection of which of following hemoglobins?HbAHbA2HbFHbS  <response>: The major red cell hemoglobin in thalassemia major is HbF a2 g2, which is markedly elevated. Thalassemia minor trait is associated with increased levels of HbA2 a2 d2 HbA a2 b2 accounts 95 to 98 of the total hemoglobin in normal adults. HbS is seen in sickle cell anemia. These hemoglobins can be detected by Hb high performance liquid chromatography HPLCcapillary electrophoresis. 