<prompt> Klinefelter syndrome is diagnosed by:USG abdomenEchocardiographyTriple testKaryotyping  <response>: Klinefelters syndrom XXY can be detected by karyotyping Karyotyping Study of chromosome Best technique for MonosomyTrisomy But can be only done during Metaphase Sample used: Blood, Bone marrow, Amniotic fluid, Placental tissue Fixative : Carnoys fixative Methanol and Glacial Acetic acid in ratio 3:1 Most common stain: GeimsaG-banding Limitations Cant be done in any phase of cell cycle Cant detect amplifications, microdeletions and complex translocations Low resolution i.e. subchromosomal alterations cant be detected 