<prompt> DNA fragments formed by the action of Restriction Endonucleases, are separated by:Gel electrophoresisAgarose gel eletrophoresisPaper ChromatographyHigh pressure liquid chromatography  <response>: B i.e. Agarose gel electrophoresis - Restriction endonuclease restriction enzyme makes 2 incisions through sugar phosphate backbone phospho diester bond of each strand of ds DNAQ at specific sequence ka recognition sequences or restriction site. So RE restricts viral bacteriophage - DNA replicationQ and protects bacteria prokaryote from infection by virusQ. - RE cuts both strands of double stranded ds DNA at specific restriction sites with palindromic inverse repeat arrangement; thus producing smaller, manageable fragments with sho sequenes and sticky blunt endsQ. These restriction fragments can be isolated by agarose gel polyacrylamide - electrophoresisQ or HPLC. After digestion by restriction endonucleases the DNA ends can be ligated joined annealed by DNA-ligaseQ 