<prompt> The peripheral blood smear of a patient shows features of thalasemia, also presented with anaemia. Family history is also ve. The investigation done to establish the diagnosis is -ESR estimationBlood spherocyte estimationBone marrow aspirationHb-electrophoresis  <response>: Ans. is d i.e., Hb - Electrophoresis o Hemoglobin electrophoresis should always be the first investigation to includeexclude the diagnosis of thalasemia. o The level of normal adult hemoglobin HbA is markedly decreased with propoionate increase in HbA2 and HbF. X-ray findings in thalassemia Crew cut appearance Hair on end appearance 