<prompt> In glycogen metabolism, amylo 1-6-glycosidase acts on glycogen to produce?Glucose-l-phosphateGlucose-6-phosphateMaltoseGlucose  <response>: Ans. d Glucose; Ref Harper, Biochemistry, 26th ed., 145; Satyanaryan, Biochemistry, 2nd ed., 273GLYCOGEN Glycogen contains alpha-1-4 and alpha-1-6 links. Degradation of glycogen to glucose involves 4 enzyme reactions:- Phosphorylase, which hydrolyses alpha-1-4 bond- Amylo 1-6-glycosidase, a debranching enzyme that removes alpha-1-6 bond to produce free glucose in muscle.- Phosphoglucomutase, which converts glucose-1-phosphate to glucose-6-phosphate.- Glucose-1-phosphatase, which converts glucose-6-phosphate to glucose. Thus, amylo 1-6-glycosidase debranching enzyme breaks alpha-1-6 bond at branch with single glucose residue and releases free glucose. 