<prompt> Which of the following can be given as a single weight-based intravenous bolus over 10 seconds?Tissue plasminogen activator tPATenecteplase TNKReteplase rPAAny of the above  <response>: .Tenecteplase is an enzyme used as a thrombolytic drug. Tenecteplase is a tissue plasminogen activator produced by recombinant DNA technology using an established mammalian cell line. Tenecteplase is a tissue plasminogen activator tPA produced by recombinant DNA technology using an established mammalian cell line Chinese hamster ovary cells. Tenecteplase is a 527 amino acid glycoprotein developed by introducing the following modifications to the complementary DNA cDNA for natural human tPA: a substitution of threonine 103 with asparagine, and a substitution of asparagine 117 with glutamine, both within the kringle 1 domain, and a tetra-alanine substitution at amino acids 296-299 in the protease domain. Tenecteplase is a recombinant fibrin-specific plasminogen activator that is derived from native t-PA by modifications at three sites of the protein structure. It binds to the fibrin component of the thrombus blood clot and selectively conves thrombus-bound plasminogen to plasmin, which degrades the fibrin matrix of the thrombus. Tenecteplase has a higher fibrin specificity and greater resistance to inactivation by its endogenous inhibitor PAI-1 compared to native t-PA. pharmacokinetics: Distribution: approximates plasma volume Metabolism: Primarily hepatic Half-life elimination: Biphasic: Initial: 20-24 minutes; Terminal: 90-130 minutes Excretion: Clearance: Plasma: 99-119 mLminute Ref Robbins 9e pg 234 