<prompt> Ion exchange chromatography is based on -SizeChargeSolubilityPolarity  <response>: Ans. is b i.e.. Charge Property of protein usedMethodProtein solubilitySalting out or salt extraction precipitation of protein by ammonium sulfateMolecular sizeo Gel filtration chromatography molecular-sieve chromatography or size exclusion chromatographyo Ultracentrifugationo SDS-PAGE Sodium dodecvl sulphate-polyacry lamide gel electrophoresiso DialysisMolecular charge Ionic chargeo Ion exchange chromatographyo High performance liquid chromatography HPLCo Electrophoresis simple on cellulose acetate starch gelo Isoelectric focusing a variant of electrophoresisMolecular charge and molecular w eightPAGE : polyacrylamide gel electrophoresisAffinity bindingAffinity chromatographyHvdnophobidtyHydrophobic interaction chromatography 