<prompt> PCR is used to :Detect target plasmidsAmplify small amount of DNASeal the cut ends of DNACleave the bacterial plasmid  <response>: Polymerase chain reaction PCR - used to amplify small amount of DNA In 1983, Kary Mullis developed a new technique that made it possible to synthesize large quantities of a DNA fragment without cloning it. Basic Steps involved in PCR are: a DNA extraction from microorganism b Amplification of extracted DNA- 3 steps -Denaturation 95degC, Primer annealing 55degC, Extension 72degC c Post amplification analysis by different methods like Gel electrophoresis. 