<prompt> A 16-year old girl presents with primary amenorrhea, on examination has a blind vaginal pouch with absence of uterus. Investigation to be done isProlactin levelsKaryotypingIVPFSH levels  <response>: The patient with primary amenorrhea, here is phenotypic female with absent vagina and uterus 3 possibilities here Mullerian agenesis : 46 XX Testicular feminization : 46 XY Swyer syndrome : 46 XY A Karyotyping will be the best to differentiate between the three. MRKH Androgen insensitivity syndrome Genotype XX XY Gonad Ovaries Testes Breast development Normal Normal Pubic and axillary hair Normal Absent insensitivity to testosterone Vagina Shallow and blind Shallow and blind Uterus Absent Absent Vulva Normal Normal Sr Androgen levels 20-80 ngdl 200-800 ngdl 