<prompt> Pneumocystis carinii is diagnosed bySputum examination for trophozoites and cysts under microscopeCulturePositive serologyGrowth on aificial media  <response>: Pneumocystis infection - specimen is induced sputum, bronchoalveolar lavage or lung biopsy. On microscopy - trophozoites can be demonstrated by Giemsa , toludine blue, methenamine silver and calcofluor white stain. Ref: Textbook of Microbiology, Ananthanarayan and Paniker; 9th edition 