<prompt> Lyophilised Freeze dried vaccine do not include:-BCGOPVMeaslesJE Live vaccine  <response>: Lyophilized freezed dried vaccine:- Available in Powder form Examples:- BCG Yellow Fever Japanese Encephalitis HiB Require Saline Measles MMR Distilled water saline water - Reconstructed vaccines should be used before 4hrs other wise they may case Toxic shock syndrome. 