<prompt> The rate-limiting enzyme in glycolysis isGlucose 6-dehydrogenasePhosphofructokinaseGlucokinasePyruvate kinase  <response>: Phosphofructokinase PFKstep3 is the most impoant rate-limiting enzyme for glycolysis pathway. Step 3The enzyme phosphofructokinase uses another ATP molecule to transfer a phosphate group to fructose 6-phosphate to form fructose 1, 6-bisphosphate.Fructose 6-phosphate C6H11O6P1 phosphofructokinase ATP - ADP Fructose 1, 6-bisphosphate C6H10O6P2 Ref: DM Vasudevan, 7th edition, page no: 113 