 Analysis of Glucose, C-peptide, and Insulin: Concentrations of glucose, insulin, and C-peptide will be analyzed in the UAB Diabetes Research Center Core Director: B. Gower, Primary Mentor. Glucose will be measured in 3 μL sera using the Glucose oxidase method on a Stanbio Sirrus analyzer Stanbio Laboratory, Boerne, TX. Insulin will be assayed in 50-μL aliquots using immunofluorescence on a TOSOH AIA-II analyzer TOSOH Corp, S. San Francisco, CA. C-peptide will be assayed in 20-μL aliquots using the TOSOH analyzer. 