 Statical Analysis Statistical analysis was applied using SPSS 19.0 statistical software.our sample size calculation method is dixon and massey up and down method. According to this account, n 2 SD SEM 2; n: sample size, SD standard deviation: The standard deviation, SEM standard error of mean is calculated as the standard error of the mean SD 5 mL, SEM 1.0 mL n were found in 50 patients. For the aplicational errors we add 5 patient 10 . A total of 55 patients were included in the study. 