 Method: We analyzed 46 patients, both sexes, who filled out a questionnaire about their age and habits sport activity and smoking. They were then divided into two groups. Group 1 consisted of patients with normal BMI range from 18,5 to 24.9 n27 and Group 2 had overweight patients with BMI index 25 to 29.9 n19. There were no underweight or obese patients. All 46 patients had the ADRCS extracted for therapeutical use, but with different indications. The mixture of 180 mL saline solution, 1mL Epinephrine 1:10,000 and 20 mL of 2 lidocaine is injected in the subcutaneous layer of the abdominal wall to allow the tumescence and to decrease the blood loss. The manual liposuction procedure has been carried out through two or three small incisions using harvester Tulip medical products, San Diego, USA. All of them had liposuction done by the author and in the same manner tumescent technique, same cannula to obtain 120-200ml of fat. The harvest site is then covered with a pressure dressing for 10 days to reduce swelling and hematoma. The closed system PureGraft 250mL PureGraft, Solana Beach, California, USA has been used used to collect the specimen. The specimen is transferred within 15 min to the BelPrime Clinic laboratory where the Celution 800CRS system Cytori Therapeutics, Inc. San Diego, California, USA is applied to process the lipoaspirate in order to obtain ADRCs. The Celution 800CRS system is a closed automated medical device that helps to separate blood and fat. The system will then digests fat using the proprietary enzyme reagent Celase 835CRS Cytori Therapeutics, Inc. San Diego, California, USA to release the stromal vascular fraction SVF. SVF is subsequently concentrated by a short centrifugation and then automated wash cycles to obtain the ADRC fraction. This 90 to 120-minute process provides approximately 5mL of pellet. A few drops of the obtained pellet were immediately used for cell counting ChemoMetec AS DK-3450, Allerod, Denmark. 