 The investigators will enroll 80 participants, both male and female with the goal of having 20 paired data of responders and 20 non-responders each. Forty age-matched healthy controls will also be recruited. The investigators will recruit 100 subjects anticipating drop outs or inability to participate fully with microbiome sample collection and or at least paired biological specimens before and after treatment. The Mayo Clinic Jacksonville patients n10 will be matched with Mayo Clinic Jacksonville controls n10 and the Mayo Clinic Rochester patients n30 will be matched with the Mayo Clinic Rochester controls n30. 