 Study Protocol Cell Culture Bone marrow and tissue samples will be obtained from the patients after they will give their written informed consent. Material will be maintained in RPMI culture medium Sigma, R0883, Germany. Peripheral blood mononuclear cells PBMCs from healthy donors will be used as control. For detection of cancer cells in our samples we perform flow cytometry using EpCAM magnetic beads 39-EPC-50; Gentaur, and the negative selection cells non-cancerous are isolated and then cultured in a 25-cm2 flask 5520100; Orange Scientific with RPMI-1640 medium R6504; Sigma. 