 4. ESAT6-CFP10 10μgmlin right arm; Inject intradermally ESAT6-CFP10 and TB-PPDtuberculin purified protein derivative in different arms of the same subject.For each of the participants in this clinical research, this study uniform that left arm inject a drugESAT6-CFP10 or TB - PPD first, observe 30 min and no no obvious adverse reaction ,then another drugESAT6-CFP10 or TB - PPD inject in right arm. We need draw blood to detect specific γ- IFN before the injection.Observe and record the vital signs breathing, heart rate, blood pressure and temperature,the skin reaction diameter of injection site flush and induration , local reactions rash, pain, itching and skin mucous membrane and a variety of adverse events. 