 -Procedure: The patient is placed in the lateral decubitus position, with the top leg flexed and the lower leg straight. The iliac crest is palpated, and the preferred sampling site is marked with a pen. Aseptic technique is employed, including sterile gloves and gown. The site is prepared with an antiseptic povidone-iodine, scrubbed, and draped, exposing only the site to be sampled. The skin and the underlying tissue to the periosteum are infiltrated with a local anaesthetic approximately 10 mL of 1 Xylocaine lidocaine. A 10-mL syringe with a 25-gauge needle is used to inject an initial 0.5 mL directly under the skin, raising a wheal. A 22-gauge needle is used to penetrate deeper into the subcutaneous tissue and the underlying periosteum, an area roughly 1 cm in diameter. A skin incision is made with a small surgical blade, through which the bone marrow aspiration needle, with a stylet locked in place, is inserted. Once the needle contacts the bone, it is advanced by slowly rotating clockwise and counter clockwise until the cortical bone is penetrated and the marrow cavity is entered. The depth of the penetration should not extend beyond an initial 1cm. Once within the marrow cavity, the stylet is removed. Using a 20 mL syringe, approximately 5 mL of bone marrow are aspirated. Subsequent specimens are obtained by attaching a separate syringe, collecting 5 mL at a time. The samples are then transferred into an ethylenediaminetetraacetic acid EDTA -containing tube. The marrow needle is removed, and pressure is applied to the aspiration site with gauze until any bleeding stops. 