 Significant controversy surrounds the possibility that consumption of artificial sweeteners AFS leads to weight gain. Some studies have found correlations between AFS use and weight gain andor diabetes 1-4 while others have indicated that AFSs may aid in weight loss 5 or have no effects on body mass index BMI 6. In rats, exposure to AFS leads to reduced chow intake following a sweet preload 7, 8, higher body weight 9, 10 and increased glucose responses and decreased GLP1 release following an oral glucose tolerance test 11 compared to exposure to caloric sweeteners. Given that the five FDA approved AFSs are found in thousands of foods 12 this marks a clear and significant gap in knowledge. Our preliminary data demonstrate a 3-fold decrease in sweet taste sensitivity following consumption of a beverage sweetened with two packets of Splenda for just 10 days. These data provide strong evidence that repeated exposure to sucralose reduces perception of sweet taste intensity, most likely by down-regulation of the sweet taste receptor. Therefore, it is imperative that we gain a greater understanding of the consequences of AFS use, since alterations in sweet taste perception that occur in response to AFS exposure may promote weight gain. 