 : The autologous adipose derived mesenchymal stem cell as the investigational product 2 x 107 cellsmL vial or 4 x 107 cellsmL vial and Tissuefill 1mLsyringe are supplied to the operating room. The investigational product and the Tissuefill hyaluronic acid derivatives are mixed and placed in a 22 G syringe. A single dosage of 2x107 autologous adipose derived mesenchymal stem cells per person is administered on 5 subjects, and a single dosage of 4x107 cells per person is administered on 5 subjects, injected into the center of the nucleus pulposus. 