<prompt> Please answer with one of the option in the bracket. Write reasoning in between . Write answer in between .Q:A 16-year-old female presents to the physician for delayed onset of menstruation. She reports that all of her friends have experienced their first menses, and she wonders whether something is wrong with me. The patient is a sophomore in high school and doing well in school. Her past medical history is significant for an episode of streptococcal pharyngitis six months ago, for which she was treated with oral amoxicillin. The patient is in the 35th percentile for weight and 5th percentile for height. On physical exam, her temperature is 98.7F 37.1C, blood pressure is 11267 mmHg, pulse is 71min, and respirations are 12min. The patient has a short neck and wide torso. She has Tanner stage I beast development and pubic hair with normal external female genitalia. On bimanual exam, the vagina is of normal length and the cervix is palpable.Which of the following is the most accurate test to diagnose this condition?? A: Buccal smear for Barr bodies, B: Karyotype analysis, C: Serum FSH and LH levels, D: Serum testosterone level, E: Serum 17-hydroxyprogesterone level,  <response>: This is a question about a 16-year-old female with delayed onset of menstruation. The key findings in the stem are:- Delayed menarche compared to peers- Short stature and wide torso - Tanner stage I breast development- Normal external genitalia and vaginal lengthThis clinical presentation is most consistent with Turner syndrome, which is characterized by short stature, webbed neck, wide chest, ovarian dysgenesis and primary amenorrhea in phenotypic females. The most accurate test to diagnose Turner syndrome is karyotype analysis, which would reveal 45,XO genotype.Buccal smear detects Barr bodies, which are inactivated X chromosomes and not useful here. FSH, LH and testosterone levels help evaluate ovarian function but do not diagnose Turner syndrome. 17-OHP is elevated in congenital adrenal hyperplasia, which does not fit the clinical picture here.Therefore, the best answer is karyotype analysis.B: Karyotype analysis 