<prompt> Please answer with one of the option in the bracket. Write reasoning in between . Write answer in between .Q:A 61-year-old male is given acetazolamide to treat open-angle glaucoma. Upon diuresis, his urine is found to be highly alkaline. Which of the following accounts for the alkaline nature of this patients urine?? A: Inhibition of chlorine reabsorption in the thick ascending loop of Henle, B: Inhibition of chlorine reabsorption in the distal convoluted tubule, C: Inhibition of acid secretion in alpha-intercalated cells, D: Inhibition of bicarbonate reabsorption in the proximal tubule, E: Inhibition of bicarbonate reabsorption in beta-intercalated cells,  <response>: This is a question about the mechanism of action of acetazolamide and how it affects urine pH. Acetazolamide is a carbonic anhydrase inhibitor that inhibits the enzyme carbonic anhydrase in the proximal convoluted tubule of the nephron. This decreases the reabsorption of bicarbonate, causing more bicarbonate to be excreted in the urine. Bicarbonate is a base, so increased excretion of bicarbonate makes the urine more alkaline. D: Inhibition of bicarbonate reabsorption in the proximal tubule 