<prompt> Please answer with one of the option in the bracket. Write reasoning in between . Write answer in between .Q:DNA replication is a highly complex process where replication occurs on both strands of DNA. On the leading strand of DNA, replication occurs uninteruppted, but on the lagging strand, replication is interrupted and occurs in fragments called Okazaki fragments. These fragments need to be joined, which of the following enzymes is involved in the penultimate step before ligation can occur?? A: DNA ligase, B: DNA gyrase, C: DNA helicase, D: DNA polymerase I, E: DNA polymerase III,  <response>: This is a question about DNA replication, specifically about the process of joining Okazaki fragments on the lagging strand. The key point is that Okazaki fragments need to be joined before final ligation by DNA ligase can occur. The question asks which enzyme is involved in the penultimate next to last step before ligation. Based on knowledge of DNA replication, the enzyme that joins Okazaki fragments is DNA polymerase I. It fills in the gaps between fragments by removing the RNA primers and replacing them with DNA nucleotides. This prepares the DNA for the final step of ligation by DNA ligase.So the enzyme involved in the penultimate step before ligation of Okazaki fragments is DNA polymerase I.D: DNA polymerase I 