<prompt> Please answer with one of the option in the bracket. Write reasoning in between . Write answer in between .Q:A 45-year-old woman with β-thalassemia major comes to the physician with a 1-week history of fatigue. She receives approximately 8 blood transfusions per year; her last transfusion was 1 month ago. Examination shows conjunctival pallor. Her hemoglobin level is 6.5 mgdL. Microscopic evaluation of a liver biopsy specimen in this patient would most likely show which of the following?? A: Macrophages with yellow-brown, lipid-containing granules, B: Macrophages with cytoplasmic granules that stain golden-yellow with hematoxylin, C: Extracellular deposition of pink-staining proteins, D: Cytoplasmic brown-pigmented granules that stain positive for S-100, E: Cytoplasmic pink-staining granules that stain positive with PAS,  <response>: This is a question about a patient with beta-thalassemia major, which is a genetic disorder that results in reduced production of hemoglobin. The patient has a low hemoglobin level indicating anemia. The question asks what would most likely be seen on microscopic evaluation of a liver biopsy in this patient. The choices describe different types of pigmented or stained deposits that could be seen in liver cells or macrophages. To determine which one is most likely in this patient, we need to consider the pathophysiology of beta-thalassemia and how it affects the liver. In beta-thalassemia, the chronic anemia leads to increased iron absorption from the diet. Over time this causes iron overload, with deposition of excess iron in tissues like the liver. The iron builds up in liver macrophages, causing them to appear brown on biopsy.So the correct answer should describe brown pigmented deposits in liver macrophages, indicative of iron overload.B: Macrophages with cytoplasmic granules that stain golden-yellow with hematoxylin 