<prompt> Please answer with one of the option in the bracket. Write reasoning in between . Write answer in between .Q:An investigator is studying the effects of zinc deprivation on cancer cell proliferation. It is hypothesized that because zinc is known to be a component of transcription factor motifs, zinc deprivation will result in slower tumor growth. To test this hypothesis, tumor cells are cultured on media containing low and high concentrations of zinc. During the experiment, a labeled oligonucleotide probe is used to identify the presence of a known transcription factor. The investigator most likely used which of the following laboratory techniques?? A: Western blot, B: Northern blot, C: PCR, D: ELISA, E: Southwestern blot,  <response>: This is a question asking about an experimental technique used to identify the presence of a transcription factor. The investigator is studying the effects of zinc deprivation on cancer cell proliferation by culturing tumor cells in media with low and high zinc concentrations. The hypothesis is that zinc deprivation will slow tumor growth by affecting transcription factor activity. To test this, the investigator used a labeled oligonucleotide probe to identify a known transcription factor. Of the techniques listed, Southwestern blotting answer E is used to detect specific DNA-binding proteins like transcription factors using a labeled DNA probe. PCR amplifies DNA, Northern blot detects RNA, Western blot detects proteins, and ELISA detects antigens. Therefore, Southwestern blot is the best answer.E: Southwestern blot 