 The first 20 patients will receive 4 mlkg normal clinical practice between the base line and the second measurement of Pmsf-arm. The subsequent 20 patients will receive 3mlkg between the two measurements of Pmsf-arm, and then 1mlkg will be added to complete the normal fluid challenge. The following 20 patients will receive 2 mlKg between the two measurement of Pmsf-arm, and then the remaining 2 mlkg will be given to complete the normal fluid challenge. And finally, the last 20 patients will receive 1 mlkg between the two measurements of Pmsf-arm, and 3 mlkg will be added to complete clinical practice. 