 To understand 295 gene mutation mutation status include EGFR, HER2, KRAS, BRAF, PIK3CA,ect by deep sequencing in Chinese patients with pulmonary adenocarcinoma and their relationships with the patients clinical features including sex, age, smoking history and adenocarcinoma subtype, specify the predictive significance of the genes mutations for new targeted therapies in NSCLC patients, and better understand the molecular mechanism drug resistance to EGFR-TKIs. 