 Participants will be excluded from the study or analysis if any of the following occur during the study: 1 Changes in the patient medical status or medications that met the exclusion criteria; 2 Use of antimicrobial rinses; 3 Use of irrigating devices; 4 Subject noncompliance with stent wearing during the experimental mucositisgingivitis phase andor Vitamin C use; 5 Use of anti-inflammatory drugs or antibiotics; 6 allergic history to common toothpaste or mouth rinse ingredients. 