 Aim 1: Determine and characterize the microbiota in the region surrounding the skinimplant interface. Sampling will take place over all stages of wound healing and stomal maturation and will begin with Stage 1 and Stage 2 surgeries, as well as at defined time points, and collection sites i.e., the stoma, ipsilateral and contralateral thigh skin for up to one year post surgery. This will be carried-out by using a specific swabbing technique to collect bacterial and fungal DNA and to amplify and sequence bacterial 16S rRNA and fungal 18S rRNA genes. 