 2.2. Objective 2 Quality of Life evidence: To establish the cost per recurrence prevented for this approach and assess quality of life issues. Hypothesis: FV-guided surgery can be delivered in a cost effective manner and improve the quality of life of patients 2.3 Objective 3 ScientificMolecular evidence: To assess the presence of previously validated molecular markers microsatellite analysis, LOH and histological change quantitative pathology in surgical margins in a nested case-control study involving a tumor bank created within this project. Hypothesis: FV-guided surgery will spare normal tissue at the same time improving capture of high-risk tissue. 