 Diabetes is a common chronic disease with significant morbidity and mortality. One devastating complication of diabetes is peripheral arterial disease PAD, which may result in limb loss.In our study,patients were divided into 3 dose treatment groups 6.29 × 107,9.43 × 107 and 14.15 × 107 cellsm2After 4 and 8 weeks, patients were injected the same number of cells. Follow-up index include: efficacy pain,cold, numbness, Intermittent claudication distance, skin temperature,limb cyanosis area, area and depth of ulcers, gangrene range,ankle-brachial index, Transcutaneous partial pressure of oxygen ,lower limb amputation rate and , blood perfusion, Digital subtraction angiography and safety infection of the injection site, and tumour generation. 