 In the first part of the proposal, we will determine the cellular responses to various microenvironmental factors such as lactosis, acidosis, hypoxia, glucose deprivation in cultured epithelial cells commercially obtained making use of DNA microarray analysis. From the analysis of these microarray assays, we will obtain the gene signatures reflecting how cells respond to these environments stresses. These gene signatures will be used to analyze and annotate the gene expression patterns in the tumor samples and whether hyperthermia will affect the physiological parameters and the corresponding gene signatures. 