 The fat biopsies will be performed by a surgical skilled physician. After removal the biopsies are immediately transferred to ice-cold physiological buffer and transported to the laboratory at Glostrup Research Institute. At the laboratory small arterioles in the biopsies are dissected free from fat and connective tissue using a microscope. The dissected arterioles are sectioned into 1-2 mm length cylindrical segments which are either immediately used for vasomotor response studies or stored at -80C for molecular studies. For vasomotor response studies, the isolated arteriole segments are mounted in a Mulvany-Halpern wire-myograph, whereby it is possible to test the physiological response of the vessel after adding different hormones e.g. Ang II. The molecular studies consist of quantitative real-time PCR to quantify the mRNA expression of the relevant receptor genes, western blot to analyse receptor protein expression and immunohistochemistry to analyse receptor localization. 