 Flow cytometry Surface expression of MSCs using anti- CD271, CD34, CD73, CD90, CD105, CD29 monoclonal antibodies mAbs will be analyzed using flow cytometry. MSCs 2X105 cells will be suspended in PBS containing 1 BSA and will be stained with flurochrome -conjugated mAbs for 20 minutes on ice anti-mouse mAanti-CD 271, CD34 CD73, CD90, CD105,CD29; BD Bioscience, MN, USA.flow cytometric analysis will be performed using a FACSCaliber BD bioscienceequipped with cell Quest software.10000 cells will be passed in front of the laser for each sample. Each sample will be analyzed in duplicate. A cut off value at 20 will be set to categorize samples as positive. 