 Continuous variables were described as the mean and standard deviation SD or median and quartiles and categorical variables as counts and percentages. The prevalence of EEG beta activity was compared with the Fisher exact test. To test the effects of AAT on vital signs and on endocrinological parameters over time in the two groups, regression models for repeated measures were used, including a main effect for group and time, as well as their interaction. Distribution of pain scores at the end of the session in the two groups was compared with the Fisher exact test. A 2-sided p-value0.05 was considered statistically significant. All statistical analyses were performed using Stata 13.1 StataCorp, College Station, Texas, USA. 