 Routine laboratory determinations Routine laboratory determinations will be carried out by automated chemical analysis in the laboratory of the University Hospital of Ioannina using an Olympus AU 600 analyzer Olympus Diagnostica GmbH, Hamburg, Germany. Glucose will be measured by the hexokinase method and serum insulin levels by the AxSYM Insulin assay, which is based on the Microparticle Enzyme Immunoassay technology Abbott Laboratories, Diagnostic Division, Abbott Park, IL, USA. 