 3. Skeletal muscle assessment: the parameters of skeletal muscle will be assessed before and after dietary intervention for 12 months. Body weight, height, Body mass index, skeletal muscle mass will be measured by Body composition analyzer. Quadriceps strength will be assessed with a seated leg press exercise instrument Keiser Sport, Fresno, CA, USA using the five repetition maximum technique. Knee and hip extension power rate of generating force will be assessed with a validated leg power instrument University ofNottingham Medical College, Nottingham, UK. Fatigability will be assessed with the same bilateral leg press exercise used in strength testing. 