 No human trials have used doses of glibenclamide under 5mg previously in the measurement of insulin and glucagon secretion. Our sample size calculations are based on experimental data from isolated human islets from T2DM donors, which were performed in Professor Patrik Rorsmans lab. These suggest that 15 participants allowing for a 15 dropout would give the study 80 power to detect a 57 reduction in baseline glucagon levels with an alpha error of 5. 