 Half of the M. perstans infected individuals randomly selected will be treated immediately with 200mg daily doxycycline for six weeks. All recruited individuals will be asked to donate 20ml; 10 ml heparinized blood, 5ml Ethylenediaminetetraacetic acid blood and 5ml blood for serum analysis at baseline day 0, after 4 months day 112, after 12 months day 336 and after 24 months day 672. Six months after doxycycline treatment for M. perstans infected individuals the other 100 patients who are not treated at time point 0 delayed treatment group will be treated as described for the early treatment group. After four months day 112 individuals from both treatment groups will be asked to donate blood for quantitative Polymerase Chain Reaction to confirm Wolbachia depletion and immunological analyses. 