 The bone marrow aspirate will be diluted at a ratio of 6:1 with phosphate buffer saline PBS with 2 mM EDTA 30 ml BM aspirate 5 ml PBSEDTA buffer. The MNCs will be separated under aseptic conditions using a Ficoll. Hypaque desity gradient density 1.077, GibcoBRL, Grand Islan, NY, USA by centrifugation at 1800 rpm for 20 min then the MNCs will be plated in 40 ml alpha-modi-field Eagles medium αMEM, serum free media; mesencultMesenchymal stem cell culture,penicillin 100 Uml,streptomycin10 mgml,0.5 ml amphotericin Ball from Gibco BRL and 10 ngml basic fibroblast growth factor b-FGF RD system, Minneapolis, MN and will be incubated at 370 c in a humidified atmosphere containing 5 CO2 Digirolamo et al.1999.after one day ,non adherent cells will be cultured in the presence of Mesenchymal media for 3 weeks changed every 1 week cambrex Bioscience ,Nottingham, uk. After reaching 80 confluence the MSCs will be placed in 10 ml saline and will be infused intravenously 