 According to diamond concept of bone healing, there are four main factors that influence the bone healing. These factors are osteogenic factor mesenchymal stem cell, osteconductive factor HA-CaSO4, osteoinductive factor bone morphogenetic protein-2 and mechanical component implant. The potency of each component in fracture healing without bone defect has been proved in many studies. The combined effect of these components is often studied as well, but not in critical-sized bone defect. 