 Methods: Thirty cases untreated chronic periodontitis; 15 generalized moderate and 15 generalized severe and 30 controls probing pocket depths 3 mm, vertical probing attachment level 2 mm at 30 of sites were examined periodontally. Further, the activated matrix metalloproteinase-8 test was performed. The test kit becomes positive with 25 ngml activated matrix metalloproteinase-8 in the sample. 