 Design dual-center Second Hospital of Shandong University and Shandong Cancer Hospital Affiliated to Shandong University prospective clinical contrast trials by selecting the preoperative patients with esophageal squamous cell carcinoma in clinical stage II, III. Complete the work of patients screening, informed consent, preoperative preparation, surgery, neoadjuvant chemotherapy and follow-up visits under the guide of Standardized treatment program. Detect the CTCs by extract the peripheral venous blood at the time of the day before initial treatment neoadjuvant chemotherapy or surgery, one week after surgery and the follow-up visits of 3 months, 6 months, 1 year, 2years after surgery. The main evaluation index of the study is the change of CTCs in peripheral blood and the survival period of disease-free of patients, with secondary evaluation index including the surgery effect, pathology, recurrence rate of the patients, long-term survival as well as other prognostic indicators. Assess the guidance of CTCs detection towards neoadjuvant chemotherapy in the treatment of esophageal squamous carcinoma in stage II, III. 