 8. Sample size: Sample size for this study was estimated using results of a previous study which reported complete recovery of encephalopathy in 79 patients in LOLA group and 55.6 patients in the placebo group 25. At 80 power and a 5 significance level two-sided, 71 patients in each group would be required to detect a 22 difference in proportions. Assuming a dropout rate of 15 approximately 28, we will need to enroll 164 patients, i.e. 82 in each treatment arm. 