 Urine biomarkers of exposure and effect were assessed from 24-hour urine collections from the morning of Day -1 to the morning of Day 1 and from the morning of Day 5 to the morning of Day 6. Urine biomarkers of exposure included NNAL, nicotine equivalents nic 5, 3-HPMA, CEMA, 1-OHP, NNN, MHBMA, and S-PMA. The urine biomarker of effect included F2-isoprostane 8-iso-PGF2 Type III. Biomarker concentrations were adjusted for urine creatinine concentrations. 