 The image observed through the microscope lenses will be captured by the video camera and displayed on the chosen monitor. The Neubauer chamber will be then placed under the optical microscope, using an objective which magnifies the original size of the object by 400x or by any other magnification elected by the researcher. Finally, the area under the high magnification field 400x magnification is estimated in mm2 by counting the total number of individual small 0.0025 mm2 squares of the chamber within the magnification area chosen. The total area under high magnification corresponded to 0.094 mm2. 