 All the subjects received oral hygiene instructions and full-mouth scaling were performed 1 month before surgery. They were instructed to perform a non-traumatic brushing technique Roll using an ultra soft toothbrush. In twenty subjects mean age 37.10 1.03, 20-45years, 13 females, 7 males, one side of the jaw received CAF control sites 59 defects, the opposite site received CAFCGF test sites 60 defects. 