 - If participation of acceptance signed informed consent form: 3 bacteriological samples: Sputum usually done, oral wash with water before washing made specifically for this study. No change in the patients medical care treatment, monitoring, etc .... Clinical Data Collection: criteria for COPD, pulmonary function, dyspnea and sputum symptoms, assessed by the MMRC scale, quality of life assessed by St Georges respiratory questionnaire, antibiotic therapy and hospitalization in the previous 12 months . Revaluation 12 months after sampling as part of the regular monitoring of patients idem as previously. All the samples will be sent to the clinical laboratory of bacteriology CHU Reims as usually done for samples: separation of sample into 2 parts: 1 part stored at -80 C and 1 part commonly analyzed. Then, patients will be classified as PA or PA non colonized based on sputum analysis. Mutation in oprD will be characterized in a second step to classify patients as PA or PA OprD mutant carriers. Samples of all patients colonized with PA and on non PA colonized patients randomly selected which have been stored at -80C will be sent to the PEGASE plateform of Institut Pasteur de Lille. Characterization of pulmonary microbiota will be performed by high-throughput sequencing on Illumina MiSeq allowing to reach 24 million reads in 2x300 bp paired-end. 