 Venous blood samples are collected by venipuncture into two 4.5-mL draw BD vacutainer tubes containing 0.105M buffered sodium citrate 3.2 at 08.00 hours in the morning after taking the medicine. Platelet-rich plasma PRP from participants is mixed with increasing proportions of that extracted from fresh platelet sample reserved in blood bank for one day as the control for each treated subject. The proportion of control platelets mixed with inhibited platelets is calculated based on platelet numbers, starting at 10 and increasing by 10 increments. So as the PRP mixed with fresh platelet sample preserved for four days. 