 121 obese or overweight patients were enrolled in the study and 23 patients were dropped out 43 male, 55 female, aged18 years, BMI24 kgm2 from the outpatient clinic of endocrinology and metabolism department of Southern Medical University Affiliated ZhuJiang Hospital. All the subjects were randomly assigned to experiment group and control group. The experimental group was given individual instructions on how to follow the VLCD very low carbohydrate diet. Energy intake was restricted to less than 800kcalday 3349kJd carbohydrate intake 20gd. All daily meals were replaced as follows: a cup of soybean milk 200 mL and a boiled egg at breakfast; a diet nutrition bar 106 Kcal: 2.8 g carbohydrate, 11.2 g protein and 5.6 g fat; Nutriease Health Technology Co., Ltd., Hangzhou, China, nonstarchy vegetables 200 kcal, and 50 g protein from meat i.e., beef, lean pork, skinned chicken, fish at lunch and dinner. Supplementation of multivitamins and minerals was provided per day. The energy-restricted diet ER diet was designed in the traditional Chinese style with an initial target for a total energy intake of 1200 kcald 5021 kJd. Subjects were also encouraged to drink at least 1.8 litres of water per day, and asked to maintain their habitual level of physical activity. Compliance with the diet and physical activity level was checked at weekly visits. 