 Forty five patients will include were allocated by random or by chance to receive several clinical interventions and one of these interventions was control Group A where the patients did not receive any intracanal medication, Group B where the patients receive intracanal medication calcium hydroxide CALCIPAST Paste, Poland and chlorhexidine 2 gel. Gluco-Chex 2, Cerkamed, Poland, Group C calcium hydroxide paste only CALCIPAST Paste Poland. 