 Participants who satisfy the inclusionexclusion criteria will be enrolled into the study and assigned to the healthy cohort. The sputum samples will be collected at the study site or at the Participants home after explanation of the collection procedure by study staff. Samples will be identified with an identification number blinding the sample identity. Each subjects sputum specimen will be processed onto 13 microscope slides at the University of Texas Health Science Center of San Antonio UTHSCSA Laboratory. The UTHSCSA laboratory will process each sputum sample onto slides using the ThinPrep T2000 processor for use in the CyPath Early Lung Cancer Detection Assay. 