 Anti-CD3-FITC fluorescein isothiocyanate, anti-CD8-PE phycoerythrin, anti-CD4-PE, anti-IFN-γ and anti-IL-4, anti-CD4, anti-CD8 and controls IgG-FITC and -PE will be used for labeling cells. Fluorescein isothiocyanate FITC a reactive derivative of fluorescein, has been one of the most common fluorophores chemically attached to other, non-fluorescent molecules to create new fluorescent molecules for a variety of applications. DAKO system will be used. For staining, immunochemistry will be used after plating the cells on Superfrost Plus slides by cytocentrifugation with the kit of DAKO according to instructions of the manufacturer. 