 We used confidence intervals CIs with a prespecified non-inferiority margin of 10 for the non-inferiority analysis. We concluded non-inferiority of PC25 to PC22 if the lower limit of the 95 CI for the difference PC25-PC22 was not lower than -10. It was assumed that the diagnostic accuracy of one pass was 75 and that of three cumulative passes was estimated to be 98.4 in PC22. Based on these assumptions, the calculated sample size was 216 samples with power of 80 and significance level of 5. 