 - MGMT Promoter Methylation testing will be performed by BWHs CLIA-approved Molecular Diagnostics and Cytogenetics Laboratory in the Center for Advanced Molecular Diagnostics CAMD; there they will utilize their standard clinical two-step methylation specific polymerase chain reaction PCR assay to detect DNA methylation within the promoter region of the MGMT gene. Fully methylated and unmethylated control DNAs are run concurrently with each assay to ensure quality control 