 Microsatellite instability is more common in colorectal cancer CRC young patient which is associated with good prognosis and is considered as a predictor for good response to preoperative chemoradiotherapy. Counting of cluster of differentiation CD 133 ve cells ,as a marker for enrichment with colorectal cancer stem cells ,is considered as a prognostic marker for poor survival and predictor for radio-resistance. Correlation between microsatellite status MS and CD133 count has not yet studied especially in young patients with rectosigmoid cancer. So the investigators hypothesize that there is correlation between microsatellite status, CD133ve cells count , occurrence of CRC in young patients and resistance to standard treatment regimen. Improvement of response to treatment and choice of the best regime to avoid non beneficial treatment modality are the goal of this study. 