 The level of master transcription factors are responsible for the shift of Cluster Differentiation CD 4 T cells into different phenotypes: T-bet for Th1 cells, Trans-acting T-cell-specific transcription factor GATA-3 for Th2, Foxp3 for T-regs, RORγt retinoic acid receptor-related orphan nuclear receptor gamma for Th17, and DX5 as a marker of Natural Killer cells. Isolated lymphocytes are saved and resuspended in 500 μL Trizol Invitrogen reagent for RNA extraction and stored in -80 C until use. The Bartholin Institute, Rigshospitalet will be responsible for the study of diet induced proportional changes in the above mentioned T cell populations. 