 4. Clinical phase The participant will be informed about the study and after its acceptance, heshe will initiate the study. Initially will be clinical examination in order to report the presence of any of the exclusion criteria already listed. After verifying the absence of these criteria, there will be a dental prophylaxis pumice for removal of this biofilm and in some cases may be made supragingival scraping. The patient will be advised not to make use of any fluoride product during the experiment. At first, volunteers are instructed to use a toothpaste without fluoride wash out the first week, in order to equate the study participants and eliminate any potential carryover effects of toothpaste used previously. 