 This prospective observation study will include 90 NCWS patients with irritable bowel syndrome IBS-like symptoms, 90 IBS and 90 celiac controls. Patients will be recruited at the Internal Medicine and at the Gastroenterology Units of the University of Palermo. Elimination diet and double-blind placebo controlled DBPC wheat challenge proved the NCWS diagnosis. All subjects underwent BMD assessment by Dual Energy X-Ray Absorptiometry DXA, duodenal histology, Human Leukocyte Antigen HLA DQ typing, body mass index BMI evaluation and assessment for daily calcium intake. 