 In each phase, following an overnight fast, an intravenous catheter will be inserted into a superficial vein in each forearm for blood sampling. After an overnight fast, subjects were admitted in the research unit for a 24-h period and received un isocaloric 900 kcal, mixed meals 75 g carbohydrates, 50 g fat 60 saturated, 35 g protein, at time points 7.00 a.m. breakfast. Blood samples will drawn before and 2, 4, 6, 8, after the meal. The area under curve AUC and incremental AUC IAUC for postprandial variables will calculate in each phase. 