 500 study population I are randomly divided into two different dose group on average, according to ESAT6-CFP10 dose5μgml or 10μgml. Do specific gamma-interferonγ-IFN detection before the skin test,then inject intradermally with 5μgml ESAT6-CFP10 or 10μgml ESAT6-CFP10 and TB-PPD at different arm of the same subject.Evaluate respectively the positive rate of three detection methods the correlation of three detection methods and the security of study population I after application for ESAT6-CFP10 . 