 In vitro data have demonstrated that a combination of steroids and tacrolimus has synergistic effects on in vitro human lymphocyte proliferation. In addition, it has previously been reported, in a pilot investigation in two subjects, that a combination regimen of pimecrolimus 1 twice a day and fluticasone propionate cream 0.05 once daily was superior to fluticasone propionate cream 0.05 once daily in the acute treatment of atopic dermatitis AD. 