 This work also highlights the presence of an unknown virus double-stranded DNA. In this project we propose 1 incorporating a bank of 150 samples from patients with diabetic foot infection in grades 2-4; 2 to study the microbial flora of a selection of 50 diabetic feet previously untreated with antibiotics for bacterial metagenomics, viral metagenomics and a minimum of 10 per culturomique; 3 to evaluate the use of targeted quantitative PCR on the most frequent new species frequency in the disease and incidentally get a sense of their role in the evolution and prognosis of the disease, including failures of and targeted antibiotic therapy on all 150 samples. 