 Statistical plan: the activity of the test product at T20min, T2 and T4 will be expressed in absolute values versus baseline T0 for the entire population and for each evaluated group 35-44 yeas, 45-54 years, 55-65 years. Moreover a comparison between groups will be carried out at each considered time. Clinical data The statistical analysis of clinical data is carried out with not parametric test, while the analysis of all instrumental data are carried out with parametric test. 