 Immunohistochemical detection of Bcl-2 protein will be performed by two pathologists. After antigen retrieval and blocking of non-specific sites with normal serum using the AvidinBiotin Blocking kit Vector Laboratories, Inc, California, USA, the sections will be incubated overnight at 4o Celsius with anti-Bcl-2 mouse monoclonal antibody 1:100, Clone 124, DAKO, Denmark. The tissue-bound antibody will be incubated for 30 min at room temperature with the Envision System. Staining will be visualized by use of Envision Kit N1587, DAKO, Denmark. The cellular staining pattern for this antibody is cytoplasmic. The presence of ER and PR will be detected as described previously. The cellular staining patterns for these antibodies are nuclear. A breast cancer specimen will be used as a positive control for the steroid receptors. 