 100 patients aged 21-40 years old, diagnosed as primary or secondary infertility due to the following causes of infertility; Pelvic Inflammatory disease, dysovulation , tubal infertility, unexplained infertility, combined infertility or due to male factor . These patients are then assigned to receive fertility treatment ICSI They undergo a 3D vaginal ultrasound before treatment to measure the junctional zone thickness and followed up after treatment to determine the rate of implantation. 