 For each study participant we recorded their age, sex, state they lived in, tumor type, tumor location, recent sun exposure during the two weeks prior to the surgery, whether or not a follicle was present on the slide, and if so the follicular extension mm from the granular layer, presence of atypia, nesting, pagetoid spread. Two slides were obtained; one stained with Mart-1; the other with HE. For both, melanocyte counts per high power field 400 X HPF magnification were obtained in three locations of the slide. The mean of the 3 counts was used for analysis, though the individual counts were considered in some data summaries see below. Classification of photo-aging according to the Glogau scale was noted by the surgeon as type1: no wrinkles; type II wrinkles in motion; type III wrinkles at rest and type IV only wrinkles. Confluence was categorized by the number of adjacent melanocytes as none, mild 2 side-by-side, moderate 3-6, or severe 7 or more. 