 There are no studies specifically addressing the altered GCF profile concurrent with the onset of myocardial infarction acutely i.e. in the first 24h and no data are currently available about GCF Neopterin and VCAM-1 levels in AMI patients with chronic periodontitis. The present study aimed to assess whether GCF levels of CP patients with AMI have an alteration in GCF levels of Neopterin and VCAM-1, also assessed whether these alterations might be related to treatment of existing periodontitis in AMI patients. A total of 60 subjects 20 CP patients with AMI, 20 systemically healthy CP patients and 20 healthy controls were included.GCF samples were analyzed baseline and after 3 and 6 months, probing pocket depthPD, clinical attachment level CAL,bleeding on probing BOP , gingival and plaque PI indices were recorded. Neopterin and VCAM-1 levels concentration and total amount were determined by enzyme linked immunosorbent assay ELISA. Baseline periodontal examination of AMI patients and 24-48h GCF collection was carried out in their hospital bed under sufficient illumination using artificial light. Within a time period of 2 months after the proceeding infarction, none of the patients had received periodontal treatment. AMI patients underwent periodontal therapy after the stabilization of their condition with the consent from same cardiologist. Periodontal disease was diagnosed based on the 1999 classification system developed by Armitage, and a preoperative periapical radiograph was taken that provided baseline data in Faculty of Dentistry. Clinical index scores and GCF sampling were recorded before, 3 and 6 months after the periodontal treatment. All selected patients underwent a 2- to 4-week initial therapy, which included comprehensive proper plaque control program, scaling, subgingival curettage and root planning in Department of Periodontology. In all patients a periodontal reevaluation was performed 4 weeks after phase I therapy, to confirm the suitability of the sites for periodontal surgery. Mucoperiosteal flap operation was performed in cases where needed. The blood samples for serum were centrifuged for 10 min at 11.00RPM separating the serum from the cells. The serum samples were then immediately divided into 0.2-0.5ml aliquots and stored at -80C until required for analysis. Samples were assayed for N and VCAM-1 using quantitative enzyme immunoassays. 