 Blood 5 mlvisit: Blood will be collected using a sterile needle and syringe in trace element free vacutainer tubes. Blood samples will be sent to Dhaka Lab on the same day in cool boxes and separated into plasma and cells, aliquot of plasma will be stored at -80C. Buffycoat cells will be stored in RNALater in -80C freezer. Whole blood 100 ul will be stored in -80C. 