 HFpEF subjects NYHA class II defined by modified Framingham criteria4 2 major or 1 major 2 minor: Major criteria: paroxysmal nocturnal dyspnea or orthopnea, jugular distension or venous pressure16 mmHg, rales or pulmonary edema, cardiomegaly, hepatojugular reflex, weight loss4.5 kg in response to diuretics, BNP400; Minor criteria: ankle edema, nocturnal cough, exertional dyspnea, pleural effusion, HR120, hepatomegaly, vital capacity23 normal, BNP200, LA volume index40ccm2. 