 - Application of E test strips Ensure that the inoculated agar surface is completely dry before applying E test strips. Use a Etest applicator or use a forceps to grip the handle of the strip are labeled E and place them on to the inoculated agar surface, ensuring that the MIC scale is facing upwards and that the concentration maximum is nearest to the rim of the plate. Make sure the whole strip is in complete contact with the agar surface. Once applied, the strip cannot be moved because of the instantaneous release of antibiotic into the agar. It is recommended to apply 4-6 different E test strips onto a 150 m agar plate. In this study, the E test strips of six antimicrobial agents, including cefepime, cefepime plus clavulanic acid for the identification of ESBL producing isolates, ciprofloxacin, cefmetazole, amikacin, tigecycline will be applied in addition to ertapenem. 