 The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines LIF 5ngml, HB-EGF 5ngml and GM-CSF 2ngml as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results. 