 Using this approach, each section prepared by the immunohistochemical method will be evaluated under the light microscope. The number of cells in the study will be counted at 400x magnification. A 10-field count will be performed in the different situations evaluated. The mean number of cells present in this 10-field count represented an area of 0.094 mm2. By means of a simple rule of three, the number of cells will be converted into 1 mm2 counts. The cell count will be expressed as numerical density, i.e. number of positive cells per square millimeter of epithelium. 