 - Urine protein must be screened by urine analysis for Urine Protein Creatinine UPC ratio. For UPC ratio 0.5, 24-hour urine protein must be obtained and the level must be 1,000mg for patient enrollment. The urine protein used to calculate the UPC ratio must be obtained within 28 days prior to registration. NOTE: UPC ratio of spot urine is an estimation of the 24-hour urine protein excretion - a UPC ratio of 1 is roughly equivalent to a 24-hour urine protein of 1gm. UPC ratio is calculated using one of the following formulas: urine proteinurine creatinine - if both protein and creatinine are reported in mgdL 