 Blood, urine and stool samples will be collected at the above time points. Changes to the microbiome will be assessed using 16S rRNA sequencing and qPCR. Selected responders and non-responders will undergo full shotgun metagenomic and metatranscriptomic analysis. Global metabonomic profiles of serum, urine and faecal water will be generated using 1H-NMR. Further targeted analyses of bile acid and short-chain fatty acid profiles will be performed using LC-MS GC-MS. 