 The primary outcome measure was change in 21-item Hamilton Depression Rating Scale HAMD, pain with Brief Pain Inventory- Long form BPI. Secondary outcome measures included changes in Beck Depression Inventory-II BDI-II, Neurotoxicity Rating Scale NTRS, WHO Quality of Life WHOQoL, Clinical Global Impression CGI, biomarkers such as neurotransmitter, kinase, protein, cytokine, DNA, mRNA related to comorbidity of chronic pain and major depression. 