 The morphometric technique for immunohistochemistry quantification will be done with the aid of a Neubauer graduated chamber. This consists of a glass slide, thicker than a regular slide, each having a grid design dividing it into squares with known dimensions, visualized only through an optical microscope. The Neubauer counting chamber is divided into 9 large squares, each with 1 mm sides, by means of triple lines thus area of a large square 1 mm2. Each large square contains 16 4 x 4 medium-size squares. Each medium size square is further divided by triple lines into 25 5 x 5 smaller squares, each with a side length of 15 of a mm. Thus, the area of an individual small square is 0.05 x 0.05 mm 0.0025 mm2 and 0.1 mm deep . 