 The aim of the study and the procedures to be undertaken will be explained to all potential subjects. Subjects will then be requested to read the subject information sheet and read and sign the informed consent form, and will receive a signed copy. The following assessments will be performed at Visit 1 Informed consent Demographics Medical and medication history Rome III questionnaire Evaluation of eligibility criteria Vital signs Physical examination, body weight, height and BMI assessment Food frequency questionnaire IBS symptom assessment scores Hospital Anxiety and Depression Scale International Physical activity Index Blood samples Stool samples 